COMMENT: 0003e68d3973e436 12 Vkc9Jfr/G/sGcr7+/8Kjbb3j9u8 ------- COMMENT: 005334747ce0c70e 7 L8nLq6HOIWMZffRR00UNMlOxDY4 Metal and acid-labile sulfide analysis of anaerobically purified Fep1-DBD from three inde- pendent samples indicated 0.76 ± 0.12 Zn, 0.69 ± 0.08 Fe, and 0.85 ± 0.10 S2- bound per monomer. ------- COMMENT: 005334747ce0c70e 10 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 005334747ce0c70e 11 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 005334747ce0c70e 12 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 005334747ce0c70e 13 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 005334747ce0c70e 14 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 005334747ce0c70e 15 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 005334747ce0c70e 16 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 005334747ce0c70e 17 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 005334747ce0c70e 18 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 005334747ce0c70e 19 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 005334747ce0c70e 20 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 005334747ce0c70e 21 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 00548851c7ec9684 1 qUSfTQzHhw5i82myNlpABLjgfu4 (0.08% Glu) that promote respiratory metabolism (Fig. 4A). We observed that the short-lived mutants lon1Δ and yta12Δ showed a severe growth defect in respiratory-prone media, comparable to that detected in cox6Δ lacking a subunit of the ETC complex IV ------- COMMENT: 00548851c7ec9684 2 M2YqNfdgHNLMDFxhbf7Sozq8JwE Lack of the mitochondrial protease Lon1 reduced longevity whereas the absence of Mgr3, adaptor protein of the protease Yme1 [13], led to increased lifespan. ------- COMMENT: 00548851c7ec9684 4 qUSfTQzHhw5i82myNlpABLjgfu4 (0.08% Glu) that promote respiratory metabolism (Fig. 4A). We observed that the short-lived mutants lon1Δ and yta12Δ showed a severe growth defect in respiratory-prone media, comparable to that detected in cox6Δ lacking a subunit of the ETC complex IV ------- COMMENT: 00548851c7ec9684 5 pQdI+7fMpyhxcBiLK8R3c6KO9cI Moreover, cells lacking the protease Yme1 displayed increased lifes- pan in a similar manner to mgr3Δ mutant, whereas the loss of the protease Yta12 resulted in a significant reduc- tion of longevity (Fig. 3B). ------- COMMENT: 00548851c7ec9684 8 az7DHgZ/GlvPoRymKvag2NkpewQ In contrast, long-lived mutants mgr3Δ and yme1Δ displayed a normal growth in low glucose conditions (Fig. 4A). ------- COMMENT: 00548851c7ec9684 9 NyJx+5uD5IgMf6UsdLYq/y2da0w Moreover, cells lacking the protease Yme1 displayed increased lifes- pan in a similar manner to mgr3Δ mutant, whereas the loss of the protease Yta12 resulted in a significant reduc- tion of longevity (Fig. 3B). This increase in the respiratory activity was more evident for yme1Δ mutant in high glucose medium (Fig. 4B, left panel). ------- COMMENT: 00548851c7ec9684 11 M2YqNfdgHNLMDFxhbf7Sozq8JwE Lack of the mitochondrial protease Lon1 reduced longevity whereas the absence of Mgr3, adaptor protein of the protease Yme1 [13], led to increased lifespan. ------- COMMENT: 00548851c7ec9684 12 az7DHgZ/GlvPoRymKvag2NkpewQ In contrast, long-lived mutants mgr3Δ and yme1Δ displayed a normal growth in low glucose conditions (Fig. 4A). ------- COMMENT: 00548851c7ec9684 15 kS3915Q6kxBsT/i8URlW2GMlYmo Interest- ingly, the short-lived mutant yta12Δ displayed aggregated mitochondria at the cell poles similar to msp1Δ cells, suggesting that this matrix protease may participate in the regulation of mitochondrial fusion (Fig. 5D, E). ------- COMMENT: 00548851c7ec9684 18 s6HtZt4lg3g2gwkygkdcFnHdbg0 cells lacking Lon1 or Yta12 displayed decreased respiratory capacity in high and low glucose (Fig. 4B) ------- COMMENT: 00548851c7ec9684 19 s6HtZt4lg3g2gwkygkdcFnHdbg0 cells lacking Lon1 or Yta12 displayed decreased respiratory capacity in high and low glucose (Fig. 4B) ------- COMMENT: 00548851c7ec9684 20 OMNRQjoX99caeewHY6xIEg99vow we detected a significant loss of ΔΨ in yta12Δ mutant and increased ΔΨ in yme1Δ cells, both results in conso- nance with our previous findings (Fig. 4C, Additional file 4: Fig. S1). ------- COMMENT: 00548851c7ec9684 21 OMNRQjoX99caeewHY6xIEg99vow we detected a significant loss of ΔΨ in yta12Δ mutant and increased ΔΨ in yme1Δ cells, both results in conso- nance with our previous findings (Fig. 4C, Additional file 4: Fig. S1). ------- COMMENT: 00548851c7ec9684 22 Do+OzTTKO6lpXqohb533U+F34Mk Fig. 4E show that the levels of Cox1, Cox2, Cox3, and Atp6 proteins are similar in wild- type and the long-lived mutants mgr3Δ and yme1Δ. However, the blots demonstrate highly reduced levels of these proteins in lon1Δ and yta12Δ mutants which might explain the respi ------- COMMENT: 00548851c7ec9684 23 Do+OzTTKO6lpXqohb533U+F34Mk Fig. 4E show that the levels of Cox1, Cox2, Cox3, and Atp6 proteins are similar in wild- type and the long-lived mutants mgr3Δ and yme1Δ. However, the blots demonstrate highly reduced levels of these proteins in lon1Δ and yta12Δ mutants which might explain the respi ------- COMMENT: 00548851c7ec9684 24 Do+OzTTKO6lpXqohb533U+F34Mk Fig. 4E show that the levels of Cox1, Cox2, Cox3, and Atp6 proteins are similar in wild- type and the long-lived mutants mgr3Δ and yme1Δ. However, the blots demonstrate highly reduced levels of these proteins in lon1Δ and yta12Δ mutants which might explain the respi ------- COMMENT: 00548851c7ec9684 25 Do+OzTTKO6lpXqohb533U+F34Mk Fig. 4E show that the levels of Cox1, Cox2, Cox3, and Atp6 proteins are similar in wild- type and the long-lived mutants mgr3Δ and yme1Δ. However, the blots demonstrate highly reduced levels of these proteins in lon1Δ and yta12Δ mutants which might explain the respi ------- COMMENT: 00548851c7ec9684 26 Do+OzTTKO6lpXqohb533U+F34Mk Fig. 4E show that the levels of Cox1, Cox2, Cox3, and Atp6 proteins are similar in wild- type and the long-lived mutants mgr3Δ and yme1Δ. However, the blots demonstrate highly reduced levels of these proteins in lon1Δ and yta12Δ mutants which might explain the respi ------- COMMENT: 00548851c7ec9684 27 Do+OzTTKO6lpXqohb533U+F34Mk Fig. 4E show that the levels of Cox1, Cox2, Cox3, and Atp6 proteins are similar in wild- type and the long-lived mutants mgr3Δ and yme1Δ. However, the blots demonstrate highly reduced levels of these proteins in lon1Δ and yta12Δ mutants which might explain the respi ------- COMMENT: 00548851c7ec9684 28 Do+OzTTKO6lpXqohb533U+F34Mk Fig. 4E show that the levels of Cox1, Cox2, Cox3, and Atp6 proteins are similar in wild- type and the long-lived mutants mgr3Δ and yme1Δ. However, the blots demonstrate highly reduced levels of these proteins in lon1Δ and yta12Δ mutants which might explain the respi ------- COMMENT: 00548851c7ec9684 29 Do+OzTTKO6lpXqohb533U+F34Mk Fig. 4E show that the levels of Cox1, Cox2, Cox3, and Atp6 proteins are similar in wild- type and the long-lived mutants mgr3Δ and yme1Δ. However, the blots demonstrate highly reduced levels of these proteins in lon1Δ and yta12Δ mutants which might explain the respi ------- COMMENT: 00548851c7ec9684 31 Meo2DN5Oc8fypBuzScsYmoIsVRg (vw: increased hydrogen peroxide formation) In contrast, the yta12Δ strain dis- played enhanced levels of basal probe oxidation (OxD0 of 0.46) at the mitochondrial matrix compared to the wild- type strain (OxD0 of 0.4), indicative of higher steady-state levels of H2O2 (Additional file 5: Fig. S2). Cells lacking Lon1 displayed a slight increase in basal oxidation of MTS-Hyper7, suggesting that both short-lived mutants show enhanced production of mitochondrial ROS (Addi- tional file 5: Fig. S2). ------- COMMENT: 00548851c7ec9684 32 ofVfNMyiH6AHVyHRpQlWFBGOLnY In contrast, the yta12Δ strain dis- played enhanced levels of basal probe oxidation (OxD0 of 0.46) at the mitochondrial matrix compared to the wild- type strain (OxD0 of 0.4), indicative of higher steady-state levels of H2O2 (Additional file 5: Fig. S2). Cells lacking Lon1 displayed a slight increase in basal oxidation of MTS-Hyper7, suggesting that both short-lived mutants show enhanced production of mitochondrial ROS (Addi- tional file 5: Fig. S2). ------- COMMENT: 00548851c7ec9684 33 xAP2+BxZm/COuJLb7eLrIBOZ/AI we have found that dele- tion of the fission genes dnm1 or fis1 caused a signifi- cant increase of longevity whereas the loss of the fusion GTPase Msp1 had no effect on lifespan (Fig. 5C). ------- COMMENT: 00548851c7ec9684 34 xAP2+BxZm/COuJLb7eLrIBOZ/AI we have found that dele- tion of the fission genes dnm1 or fis1 caused a signifi- cant increase of longevity whereas the loss of the fusion GTPase Msp1 had no effect on lifespan (Fig. 5C). ------- COMMENT: 00548851c7ec9684 35 kS3915Q6kxBsT/i8URlW2GMlYmo Interest- ingly, the short-lived mutant yta12Δ displayed aggregated mitochondria at the cell poles similar to msp1Δ cells, suggesting that this matrix protease may participate in the regulation of mitochondrial fusion (Fig. 5D, E). ------- COMMENT: 0083e32788245bc8 7 +aW2GqKFbaUi0iwJwaHIX9Ly0qg (comment: Closer to ring) ------- COMMENT: 0083e32788245bc8 8 keW0x2vEUx1I+8rB3zRaGpxlXNE (comment: Closer to ring) ------- COMMENT: 0083e32788245bc8 9 keW0x2vEUx1I+8rB3zRaGpxlXNE (comment: Closer to ring) ------- COMMENT: 009ec61b646df625 10 4o4TTdVGZAhmesV76ESp5Awyelc (comment: absent beta 1,3 gal) ------- COMMENT: 00c35706b38fef88 11 p1dwGCgy1kC6Zbre449bWyZsKSc ------- COMMENT: 00c35706b38fef88 15 JUKiF37j64aMaKpSH0OBgfVgnXQ Fig. 8A ------- COMMENT: 00c35706b38fef88 16 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: 00c35706b38fef88 17 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: 00d3dfd9f3b6d94e 9 V4QzqGelvk3/hgUdp6V4WrDOcio ------- COMMENT: 00d3dfd9f3b6d94e 10 7tl0McWCdVUttSSbLvT5JSqsCHA (comment: not shown that it is ser/thr kinase activity, just that it is kinase activity) ------- COMMENT: 00d3dfd9f3b6d94e 21 V4QzqGelvk3/hgUdp6V4WrDOcio ------- COMMENT: 00d5ca171998803c 1 FajmAL+wAiGGuTbHpnZa8Q8Me8Q (comment: mat1Msmto REIIdelta mat2::ura4 gave dark staining with iodine, metastable and switch to the opposite state at a low rate) ------- COMMENT: 00d5ca171998803c 2 LQFt1cmw9+aFEWIO2fhJsc74h3Y (comment: mat1Msmto REIIdelta mat2::ura4) ------- COMMENT: 00d5ca171998803c 3 LQFt1cmw9+aFEWIO2fhJsc74h3Y (comment: mat1Msmto REIIdelta mat2::ura4) ------- COMMENT: 00d5ca171998803c 4 sMD2HFxeMRBAuWT8HxjeZIjmyAo (comment: gave dark staining with iodine,switch to the opposite state at a low rate) ------- COMMENT: 00d5ca171998803c 5 sMD2HFxeMRBAuWT8HxjeZIjmyAo (comment: gave dark staining with iodine,switch to the opposite state at a low rate) ------- COMMENT: 00d5ca171998803c 6 LQFt1cmw9+aFEWIO2fhJsc74h3Y (comment: mat1Msmto REIIdelta mat2::ura4) ------- COMMENT: 00d5ca171998803c 10 LQFt1cmw9+aFEWIO2fhJsc74h3Y (comment: mat1Msmto REIIdelta mat2::ura4) ------- COMMENT: 00d5ca171998803c 13 LQFt1cmw9+aFEWIO2fhJsc74h3Y (comment: mat1Msmto REIIdelta mat2::ura4) ------- COMMENT: 00d5ca171998803c 20 iUHXfIrPv9XhdwzTtgjlT4ciBvU ------- COMMENT: 00d5ca171998803c 34 LQFt1cmw9+aFEWIO2fhJsc74h3Y mat1Msmto REIIdelta mat2::ura4 ------- COMMENT: 00d5ca171998803c 37 LQFt1cmw9+aFEWIO2fhJsc74h3Y (comment: mat1Msmto REIIdelta mat2::ura4) ------- COMMENT: 00e0a3ede15887bf 17 DvQxlPBfQyIsfvQGQDVqB9kfY8I (comment: can't disambiguate salt from specific calcium sensitivity in these experiments) ------- COMMENT: 00e0a3ede15887bf 18 DvQxlPBfQyIsfvQGQDVqB9kfY8I (comment: can't disambiguate salt from specific calcium sensitivity in these experiments) ------- COMMENT: 00e0a3ede15887bf 19 DvQxlPBfQyIsfvQGQDVqB9kfY8I (comment: can't disambiguate salt from specific calcium sensitivity in these experiments) ------- COMMENT: 00e0a3ede15887bf 20 DvQxlPBfQyIsfvQGQDVqB9kfY8I (comment: can't disambiguate salt from specific calcium sensitivity in these experiments) ------- COMMENT: 014aa031f039268b 1 aYdcMKp0yoRSoT0Y+mIvfu8VnjU (comment: Spindle pole body) (figure 1 a-c) ------- COMMENT: 014aa031f039268b 2 FbyhILmVMNpCokrpwO9XvRt3Pgw (comment: G2) Figure 1e, f ------- COMMENT: 0163a412b84512b3 1 rAJCr11ZwRCwftkOHl5wgCM5MKA (comment: low CoQ10 level) ------- COMMENT: 0163a412b84512b3 2 Cs4W4tYgxBqblnNIJ7gmcxPu2zU (comment: LC-MS) ------- COMMENT: 0163a412b84512b3 3 lZ+gwJHT6LX+oViv3oGCiw8g+7Y (Figs. 2A, S1A) Like other mutants lacking CoQ, the ∆coq11 strain showed better growth on cysteine-containing medium. ------- COMMENT: 0163a412b84512b3 5 3FDx3xfmNDGy18ckqgz5iQ+i2qk Figs. 3, S1C. Similar to the Δcoq2 (ppt1) strain, the Δcoq11 and Δcoq12 strains grew more slowly in the presence of 1 and 2 mM hydrogen peroxide or 0.5 mM CuSO4 than in its absence ------- COMMENT: 0163a412b84512b3 6 734uNhHm7BfktGo9t5NXggcxBy8 (Fig. 4) The results revealed higher sulfide levels in both Δcoq11 and Δcoq12 strains ------- COMMENT: 0163a412b84512b3 7 3Tgq2W5HIyya6zxcoFJp3gf/R38 The amount of Coq4 was significantly reduced in ∆coq11 and ∆coq12 single mutants ------- COMMENT: 0163a412b84512b3 8 rAJCr11ZwRCwftkOHl5wgCM5MKA (comment: low CoQ10 level) ------- COMMENT: 0163a412b84512b3 9 2apE6GRGerh42+qT9xk6PsVL/kY Coq12-GFP fusion (Fig. 8A). The GFP fluorescence pattern was similar to that of Mitotracker Red, a mitochondria stain. Mitochondrial localization of Coq12 was therefore confirmed ------- COMMENT: 0163a412b84512b3 10 KfV0by3L0APkYTLaQNP3eVqRoH4 (comment: LC-MS) ------- COMMENT: 0163a412b84512b3 11 DmupgSpDNjo1jzo5hTI5PfaOikA (Fig. 2A) By contrast, the ∆coq12 strain showed almost no growth on PMLU medium containing cysteine. ------- COMMENT: 0163a412b84512b3 15 3FDx3xfmNDGy18ckqgz5iQ+i2qk Figs. 3, S1C. Similar to the Δcoq2 (ppt1) strain, the Δcoq11 and Δcoq12 strains grew more slowly in the presence of 1 and 2 mM hydrogen peroxide or 0.5 mM CuSO4 than in its absence ------- COMMENT: 0163a412b84512b3 18 734uNhHm7BfktGo9t5NXggcxBy8 (Fig. 4) The results revealed higher sulfide levels in both Δcoq11 and Δcoq12 strains ------- COMMENT: 0163a412b84512b3 27 3Tgq2W5HIyya6zxcoFJp3gf/R38 The amount of Coq4 was significantly reduced in ∆coq11 and ∆coq12 single mutants ------- COMMENT: 0163a412b84512b3 29 IrGbeRzmN8CIi7VrDPVgQzHbjGc Interestingly, NAD+ reduction activity was clearly detected in purified Coq12-8xHis from S. pombe (Fig. 9A) ------- COMMENT: 0163a412b84512b3 33 LdssxeX+PekT5Y2ghHRzJ52IxG4 As a result, we obtained 42 strains in which the CoQ10 content was lower than half that of the wild-type (WT) strain. The 10 mutants with the lowest CoQ10 levels are listed in Table 1 ------- COMMENT: 0163a412b84512b3 34 LdssxeX+PekT5Y2ghHRzJ52IxG4 As a result, we obtained 42 strains in which the CoQ10 content was lower than half that of the wild-type (WT) strain. The 10 mutants with the lowest CoQ10 levels are listed in Table 1 ------- COMMENT: 0163a412b84512b3 35 LdssxeX+PekT5Y2ghHRzJ52IxG4 As a result, we obtained 42 strains in which the CoQ10 content was lower than half that of the wild-type (WT) strain. The 10 mutants with the lowest CoQ10 levels are listed in Table 1 ------- COMMENT: 0163a412b84512b3 36 LdssxeX+PekT5Y2ghHRzJ52IxG4 As a result, we obtained 42 strains in which the CoQ10 content was lower than half that of the wild-type (WT) strain. The 10 mutants with the lowest CoQ10 levels are listed in Table 1 ------- COMMENT: 0163a412b84512b3 37 LdssxeX+PekT5Y2ghHRzJ52IxG4 As a result, we obtained 42 strains in which the CoQ10 content was lower than half that of the wild-type (WT) strain. The 10 mutants with the lowest CoQ10 levels are listed in Table 1 ------- COMMENT: 0163a412b84512b3 38 LdssxeX+PekT5Y2ghHRzJ52IxG4 As a result, we obtained 42 strains in which the CoQ10 content was lower than half that of the wild-type (WT) strain. The 10 mutants with the lowest CoQ10 levels are listed in Table 1 ------- COMMENT: 0163a412b84512b3 39 LdssxeX+PekT5Y2ghHRzJ52IxG4 As a result, we obtained 42 strains in which the CoQ10 content was lower than half that of the wild-type (WT) strain. The 10 mutants with the lowest CoQ10 levels are listed in Table 1 ------- COMMENT: 0163a412b84512b3 40 LdssxeX+PekT5Y2ghHRzJ52IxG4 As a result, we obtained 42 strains in which the CoQ10 content was lower than half that of the wild-type (WT) strain. The 10 mutants with the lowest CoQ10 levels are listed in Table 1 ------- COMMENT: 0163a412b84512b3 41 upL/rNInOstPs1KpUToRJ/OplEU We first noticed that ∆coq11 and ∆coq12 strains did not grow well on minimal medium, as was observed for CoQ-deficient S. pombe (Fig. 2A, S1A) ------- COMMENT: 0163a412b84512b3 42 bs05rY0uxI7o146Ecmz+xCAOXno Figs. 2B, S1B ------- COMMENT: 0163a412b84512b3 43 bs05rY0uxI7o146Ecmz+xCAOXno Figs. 2B, S1B ------- COMMENT: 0163a412b84512b3 44 bs05rY0uxI7o146Ecmz+xCAOXno Figs. 2B, S1B ------- COMMENT: 0163a412b84512b3 45 bs05rY0uxI7o146Ecmz+xCAOXno Figs. 2B, S1B ------- COMMENT: 0163a412b84512b3 46 bs05rY0uxI7o146Ecmz+xCAOXno Figs. 2B, S1B ------- COMMENT: 0163a412b84512b3 47 bs05rY0uxI7o146Ecmz+xCAOXno Figs. 2B, S1B ------- COMMENT: 0163a412b84512b3 48 bs05rY0uxI7o146Ecmz+xCAOXno Figs. 2B, S1B ------- COMMENT: 0163a412b84512b3 49 bs05rY0uxI7o146Ecmz+xCAOXno Figs. 2B, S1B ------- COMMENT: 0163a412b84512b3 50 bs05rY0uxI7o146Ecmz+xCAOXno Figs. 2B, S1B ------- COMMENT: 0163a412b84512b3 51 bs05rY0uxI7o146Ecmz+xCAOXno Figs. 2B, S1B ------- COMMENT: 0163a412b84512b3 52 bs05rY0uxI7o146Ecmz+xCAOXno Figs. 2B, S1B ------- COMMENT: 0163a412b84512b3 53 3FDx3xfmNDGy18ckqgz5iQ+i2qk Figs. 3, S1C. Similar to the Δcoq2 (ppt1) strain, the Δcoq11 and Δcoq12 strains grew more slowly in the presence of 1 and 2 mM hydrogen peroxide or 0.5 mM CuSO4 than in its absence ------- COMMENT: 0163a412b84512b3 54 3FDx3xfmNDGy18ckqgz5iQ+i2qk Figs. 3, S1C. Similar to the Δcoq2 (ppt1) strain, the Δcoq11 and Δcoq12 strains grew more slowly in the presence of 1 and 2 mM hydrogen peroxide or 0.5 mM CuSO4 than in its absence ------- COMMENT: 01c913b208de3dfb 6 oNvxf8ffDjINnIEnv36lQra6R+s ------- COMMENT: 0205e2ea9f154b73 1 Rqzj0hTIQmyOH3b8nPt0zyYccPU ------- COMMENT: 020b7fc6a3fa0724 1 Gqeq4bgokS4vto+Wii//nX7w79k Figure 3 B ------- COMMENT: 020b7fc6a3fa0724 2 wwaWMaUA1zXT+X4x/CXA4D8UxxM Fig 1B Cdr1 overexpression induced hyperphosphorylation of Wee1 and loss of Cdk1-pY15, indicating inhibition of Wee1 kinase activity, ------- COMMENT: 020b7fc6a3fa0724 3 39rnVA2sFJ3sILnB8W2FdgY+rU8 Fig1B Cdr1 overexpression induced hyperphosphorylation of Wee1 and loss of Cdk1-pY15, indicating inhibition of Wee1 kinase activity, ------- COMMENT: 020b7fc6a3fa0724 4 AVZR2HVsIMux/rFSBH0fkTjGknI Fig1B. In contrast, Cdr2 overexpression induced hyperphosphoryla- tion of Wee1 but no change in Cdk1-pY15 ------- COMMENT: 020b7fc6a3fa0724 5 39rnVA2sFJ3sILnB8W2FdgY+rU8 Fig1B Cdr1 overexpression induced hyperphosphorylation of Wee1 and loss of Cdk1-pY15, indicating inhibition of Wee1 kinase activity, ------- COMMENT: 020b7fc6a3fa0724 6 W/SeexR6XIUAhtY76zPCjZKeFJU Fig 1C overexpression of Cdr1 but not of Cdr2 re- sulted in reduced cell size in cdr1∆cdr2∆ cells (Figure 1C ------- COMMENT: 020b7fc6a3fa0724 7 aBBHDQronotP9OUzjuctf0IF+zo Fig1 In contrast, Cdr2 overexpression induced hyperphosphoryla- tion of Wee1 but no change in Cdk1-pY15 ------- COMMENT: 020b7fc6a3fa0724 8 DgCTK1VwAHnLCL8w+tPe8sc8hoQ Figure 1 D Phosphorylation of Wee1 in fission yeast cells was reduced in the catalytically inactive mutant cdr1(K41A) ------- COMMENT: 020b7fc6a3fa0724 9 jB4qo/h6DQYNJ3PIoZyuL6Mzi0M Cdr1 directly phosphory- lated Wee1, but Cdr1(K41A) did not (Figure 1H). ------- COMMENT: 020b7fc6a3fa0724 10 Xh/RKhdr8ay0+Vp7QcLtSNYGjAo Figure 1H (in vitro) Cdr1 directly phosphory- lated Wee1, but Cdr1(K41A) did not (). ------- COMMENT: 020b7fc6a3fa0724 11 lbiTVFWPZzT1snCPuUbAvc5GbwM Figure 3 A onsistent with this model, wee1(4A) phosphorylation was reduced when compared with wild type, and its phosphorylation was not altered by cdr1∆ or cdr2∆ ( ------- COMMENT: 020b7fc6a3fa0724 12 NcVkiRGcJwRHIyEv1jmxzkk/S5Q deed, cdr1∆ wee1(4A) cells divided at the same size as cdr1∆ cells ------- COMMENT: 020b7fc6a3fa0724 13 EtWHIsrOSGpqk7TkvyovYacnh0g (Figure 3C) Both wee1(4A) and cdr1∆ were synthetically lethal with cdc25-dD ------- COMMENT: 020b7fc6a3fa0724 14 EtWHIsrOSGpqk7TkvyovYacnh0g (Figure 3C) Both wee1(4A) and cdr1∆ were synthetically lethal with cdc25-dD ------- COMMENT: 020b7fc6a3fa0724 15 JJlZWBBRv/V+RFinGDjg0raprKo (Figure 3, A and D). We confirmed that wee1(4A) protein level does not increase and still localizes to cortical nodes ------- COMMENT: 020b7fc6a3fa0724 16 30n81Ouada7EmhhycgjRhF+AgUI Accordingly, the size of wee1(4A) cells was largely (but not entirely) insensitive to Cdr1 overexpression (Figure 3G). ------- COMMENT: 020b7fc6a3fa0724 17 ZIx7K7Kpd9RwvjpMyCjEo7DWUfM (Figure 4A). We confirmed that S. pombe Cdk1- asM17 directly thiophosphorylates Wee1 and Wee1(K596L) ------- COMMENT: 020b7fc6a3fa0724 18 6wMllOyWgdxnwBwvrOQ+HaQ1454 Figure 5B) We tested the effects of artificially recruiting mEGFP-cdr1(∆460-482) back to nodes using cdr2-GFP- binding peptide (GBP)-mCherry, which contains the GBP. In this system, mEGFP-cdr1(∆460-482) colocalized with cdr2-GBP-mCherry at nodes. ------- COMMENT: 020b7fc6a3fa0724 19 eJaWnKYv5eAyE4Yfo+l9pq/kx7Y Along with enhanced Wee1 hyperphosphorylation, these cells di- vided at a smaller size than wild-type cells. These results show that Cdr1 localization to nodes is a limiting factor for phosphorylation of Wee1 and cell size at division ------- COMMENT: 021dd77100791f4f 1 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 021dd77100791f4f 2 YnI3sHg7ttDSlmqEiY2AgChnjTc figure3 ------- COMMENT: 021dd77100791f4f 4 8jTjtkNnNoFhiY57qk4SkW6skr0 figure 1A ------- COMMENT: 021dd77100791f4f 5 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 021dd77100791f4f 6 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 021dd77100791f4f 7 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 021dd77100791f4f 16 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 021dd77100791f4f 17 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 021dd77100791f4f 18 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 021dd77100791f4f 19 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 021dd77100791f4f 26 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 021dd77100791f4f 27 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 021dd77100791f4f 28 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 021dd77100791f4f 29 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 021dd77100791f4f 31 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 02289de0acddb1f0 1 yi11h0A4IkCjkvKD4oAf0nyanKU Supplemental Figure 4, Table 1 ------- COMMENT: 02289de0acddb1f0 2 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 3 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 4 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 5 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 6 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 7 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 8 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 9 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 10 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 11 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 12 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 13 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 14 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 15 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 16 yi11h0A4IkCjkvKD4oAf0nyanKU Supplemental Figure 4, Table 1 ------- COMMENT: 02289de0acddb1f0 17 yi11h0A4IkCjkvKD4oAf0nyanKU Supplemental Figure 4, Table 1 ------- COMMENT: 02289de0acddb1f0 18 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 19 yi11h0A4IkCjkvKD4oAf0nyanKU Supplemental Figure 4, Table 1 ------- COMMENT: 02289de0acddb1f0 20 yi11h0A4IkCjkvKD4oAf0nyanKU Supplemental Figure 4, Table 1 ------- COMMENT: 02289de0acddb1f0 21 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 22 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 23 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 24 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 25 yi11h0A4IkCjkvKD4oAf0nyanKU Supplemental Figure 4, Table 1 ------- COMMENT: 02289de0acddb1f0 26 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 27 yi11h0A4IkCjkvKD4oAf0nyanKU Supplemental Figure 4, Table 1 ------- COMMENT: 02289de0acddb1f0 28 yi11h0A4IkCjkvKD4oAf0nyanKU Supplemental Figure 4, Table 1 ------- COMMENT: 02289de0acddb1f0 29 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 30 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 31 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 32 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 33 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 34 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 35 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 36 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 37 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 38 yi11h0A4IkCjkvKD4oAf0nyanKU Supplemental Figure 4, Table 1 ------- COMMENT: 02289de0acddb1f0 39 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 40 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 41 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 42 yi11h0A4IkCjkvKD4oAf0nyanKU Supplemental Figure 4, Table 1 ------- COMMENT: 02289de0acddb1f0 43 yi11h0A4IkCjkvKD4oAf0nyanKU Supplemental Figure 4, Table 1 ------- COMMENT: 02289de0acddb1f0 44 yi11h0A4IkCjkvKD4oAf0nyanKU Supplemental Figure 4, Table 1 ------- COMMENT: 02289de0acddb1f0 45 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 46 w2N8Ubk2PksKx07iCTxDwKQpSIQ Supplemental Figure 4 ------- COMMENT: 02289de0acddb1f0 47 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 48 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 49 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 50 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 51 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 52 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 53 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 54 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 55 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 56 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 57 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 58 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 59 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 60 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 61 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 62 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 63 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 64 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 65 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 66 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 67 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 68 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 69 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 70 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 71 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 72 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 73 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 74 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 75 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 76 cb6CbrSdu9iLUAJgROFWOruGg/8 Supplemental Figure 6 ------- COMMENT: 02289de0acddb1f0 77 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 02289de0acddb1f0 78 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 02289de0acddb1f0 79 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 02289de0acddb1f0 80 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 02289de0acddb1f0 81 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 02289de0acddb1f0 82 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 02289de0acddb1f0 83 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 02289de0acddb1f0 84 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 02289de0acddb1f0 85 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 02289de0acddb1f0 86 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 02289de0acddb1f0 87 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 02289de0acddb1f0 88 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 02289de0acddb1f0 89 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 02289de0acddb1f0 90 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 02289de0acddb1f0 91 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 02289de0acddb1f0 92 yi11h0A4IkCjkvKD4oAf0nyanKU Supplemental Figure 4, Table 1 ------- COMMENT: 02289de0acddb1f0 93 yi11h0A4IkCjkvKD4oAf0nyanKU Supplemental Figure 4, Table 1 ------- COMMENT: 02289de0acddb1f0 95 uHV5w/JEFy7JMlaAqXjFO48FvPA (comment: through degradation by ubiquitination) ------- COMMENT: 02289de0acddb1f0 96 uHV5w/JEFy7JMlaAqXjFO48FvPA (comment: through degradation by ubiquitination) ------- COMMENT: 02289de0acddb1f0 97 odgO36avmvD5iqoeuoJd4APlMB0 (comment: through nuclear exclusion) ------- COMMENT: 02289de0acddb1f0 98 odgO36avmvD5iqoeuoJd4APlMB0 (comment: through nuclear exclusion) ------- COMMENT: 02289de0acddb1f0 99 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 02289de0acddb1f0 100 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 02289de0acddb1f0 101 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 025da8633ca22521 49 I2kkWZxkVIdYgx+kfxW7CID+vGY ------- COMMENT: 025da8633ca22521 50 I2kkWZxkVIdYgx+kfxW7CID+vGY ------- COMMENT: 02ba9b750a49128f 13 spnslHOpk5ouDLZypJtn0c505IY ------- COMMENT: 02e5918aa9716b93 32 mOt2IGXGza/SqxgkdDUKXSpwrYA (comment: same as hsk1-1312 alone) ------- COMMENT: 02e5918aa9716b93 34 ZmuYAD3mqjnLLzXGhVEl6AYMITU (comment: temp. restrictive for hsk1-1312 alone; fudged a bit because assayed at 32) ------- COMMENT: 02e7585390af07ef 3 fpjc4JqMAUiLBo90oS+6n8H8C7E (comment: In metaphase the difference kinds of microtubules cannot be distinguished, but they can be distinguished during anaphase B) ------- COMMENT: 02e7585390af07ef 5 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 02e7585390af07ef 6 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 02e7585390af07ef 7 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 02e7585390af07ef 8 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 02e7585390af07ef 9 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 02e7585390af07ef 10 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 02e7585390af07ef 11 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 02e7585390af07ef 12 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 02e7585390af07ef 13 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 02e7585390af07ef 14 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 02e7585390af07ef 15 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 02ecd4cbe772e63c 20 VssHis4+cBtry1WfQlmQdR3N65U (comment: vw: after attachment) ------- COMMENT: 02fb3179843288fa 5 x5qyicixhuNz37sBuL2m+bJCPEI (comment: inhibits hhf4 binding) ------- COMMENT: 02fb3179843288fa 8 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: 02fb3179843288fa 9 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: 02fb3179843288fa 10 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: 02fb3179843288fa 11 fYqQOpzikopc+3AWAEPEUkYII7w figure 4 ------- COMMENT: 02fb3179843288fa 12 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 02fb3179843288fa 13 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 0335630039399ab3 8 Am7oOM0IJUDWmEnctckThkLuBx8 ------- COMMENT: 033d6975c01e682b 1 Z5TBQOkPBvk73sXUfmd6PHDRbJ4 The enzyme is strongly inhibited by AMP and GMP. Whereas GMP seems to act by a direct competition for the GTP binding site, AMP appears as an allosteric effector, showing at non-saturating substrate concentrations a homotropic effect as well as a heterotropic effect upon the GTP and aspartate binding ------- COMMENT: 033d6975c01e682b 2 Z5TBQOkPBvk73sXUfmd6PHDRbJ4 The enzyme is strongly inhibited by AMP and GMP. Whereas GMP seems to act by a direct competition for the GTP binding site, AMP appears as an allosteric effector, showing at non-saturating substrate concentrations a homotropic effect as well as a heterotropic effect upon the GTP and aspartate binding ------- COMMENT: 033d6975c01e682b 3 Z5TBQOkPBvk73sXUfmd6PHDRbJ4 The enzyme is strongly inhibited by AMP and GMP. Whereas GMP seems to act by a direct competition for the GTP binding site, AMP appears as an allosteric effector, showing at non-saturating substrate concentrations a homotropic effect as well as a heterotropic effect upon the GTP and aspartate binding ------- COMMENT: 037e100417a6b899 1 LqF85+TfUwLxPaJIIqBcnvO2+FM Suppression of both increased fbp1 transcription and short cell length that is dependent on Gpa2 activity. ------- COMMENT: 037e100417a6b899 15 +gjo9Qy1JfMWJHkkSli7crwZZk8 (comment: Two hybrid interaction using Gpa2K270E activated protein with Sck1.) ------- COMMENT: 037e100417a6b899 29 Qn8VwG++Nh+igGAOgoonyOcNiBU Effect of sck1 deletion to increase cell length in git3 delete cells depends on Gpa2 activity. Otherwise, Sck1 acts in parallel with Pka1 to increase cell length. ------- COMMENT: 037e100417a6b899 31 +gjo9Qy1JfMWJHkkSli7crwZZk8 (comment: Two hybrid interaction using Gpa2K270E activated protein with Sck1.) ------- COMMENT: 037e100417a6b899 32 ywK6vLq4b1lrhvH41esMWDAC6ME (comment: it's a bit indirect, but they show this via consensus site mutations....I think it is borderline ok) ------- COMMENT: 037f5fa29955cd85 1 u5z44lUtT/jWtpZREhs1sL58iuE htb1-G52D, htb1-D67N and htb1-P102L can reduce the global and local abundance of H2BK119ub in cis ------- COMMENT: 037f5fa29955cd85 2 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 3 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 4 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 5 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 6 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 7 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 8 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 9 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 10 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 11 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 12 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 13 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 14 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 15 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 16 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 17 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 18 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 19 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 20 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 21 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 22 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 23 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 24 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 25 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 26 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 27 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 28 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 29 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 30 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 31 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 32 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 33 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 34 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 35 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 36 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 37 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 38 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 39 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 40 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 41 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 42 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 43 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 44 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 45 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 46 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 47 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 48 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 49 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 50 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 51 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 52 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 53 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 54 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 55 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 56 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 57 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 58 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 59 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 60 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 61 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 62 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 63 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 64 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 65 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 66 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 67 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 68 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 69 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 037f5fa29955cd85 70 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 71 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 72 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 73 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 74 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 75 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 76 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 77 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 78 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 79 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 80 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 81 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 82 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 83 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 84 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 85 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 86 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 87 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 88 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 89 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 90 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 91 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 92 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 93 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 94 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 95 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 96 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 97 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 98 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 99 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 100 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 101 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 102 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 103 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 104 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 105 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 106 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 107 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 108 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 109 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 110 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 111 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 112 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 113 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 114 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 115 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 116 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 117 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 118 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 119 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 120 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 121 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 122 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 123 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 124 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 125 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 126 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 127 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 128 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 129 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 130 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 131 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 132 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 133 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 134 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 135 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 136 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 137 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 138 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 139 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 140 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 141 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 142 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 143 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 144 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 145 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 146 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 147 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 148 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 149 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 150 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 037f5fa29955cd85 151 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037f5fa29955cd85 177 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 037f5fa29955cd85 178 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 037f5fa29955cd85 180 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: 037f5fa29955cd85 181 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: 037f5fa29955cd85 182 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: 037f5fa29955cd85 183 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 037f5fa29955cd85 184 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 037f5fa29955cd85 185 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 037f5fa29955cd85 186 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 037f5fa29955cd85 187 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 037f5fa29955cd85 188 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 037f5fa29955cd85 189 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 037f5fa29955cd85 190 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 037f5fa29955cd85 191 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 037f5fa29955cd85 192 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: 037f5fa29955cd85 193 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: 037f5fa29955cd85 194 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: 037f5fa29955cd85 195 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 037f5fa29955cd85 196 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 037f5fa29955cd85 197 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 037f5fa29955cd85 198 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: 037f5fa29955cd85 199 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: 037f5fa29955cd85 200 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: 037f5fa29955cd85 201 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 037f5fa29955cd85 202 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 037f5fa29955cd85 203 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 037f5fa29955cd85 204 gmqogAnY3BQANKEbZmiyaveRfrg (Fig. 3E) ------- COMMENT: 037f5fa29955cd85 205 gmqogAnY3BQANKEbZmiyaveRfrg (Fig. 3E) ------- COMMENT: 037f5fa29955cd85 206 gmqogAnY3BQANKEbZmiyaveRfrg (Fig. 3E) ------- COMMENT: 037f5fa29955cd85 207 DnQNbBCyjr9h1gZ1Ef6ybZH1kkk (Fig. 3F) ------- COMMENT: 037f5fa29955cd85 208 DnQNbBCyjr9h1gZ1Ef6ybZH1kkk (Fig. 3F) ------- COMMENT: 037f5fa29955cd85 209 DnQNbBCyjr9h1gZ1Ef6ybZH1kkk (Fig. 3F) ------- COMMENT: 037f5fa29955cd85 210 dJB6QAubOSayNATA9sbJUiUH0oE (Fig. 3H) ------- COMMENT: 037f5fa29955cd85 211 dJB6QAubOSayNATA9sbJUiUH0oE (Fig. 3H) ------- COMMENT: 037f5fa29955cd85 212 dJB6QAubOSayNATA9sbJUiUH0oE (Fig. 3H) ------- COMMENT: 037f5fa29955cd85 213 RAQ9iCj6ucjGCQHQQosHYrKMC00 (Fig. 3I) ------- COMMENT: 037f5fa29955cd85 214 RAQ9iCj6ucjGCQHQQosHYrKMC00 (Fig. 3I) ------- COMMENT: 037f5fa29955cd85 215 RAQ9iCj6ucjGCQHQQosHYrKMC00 (Fig. 3I) ------- COMMENT: 037f5fa29955cd85 216 vihbG/Tep2MdKhsrX63AbzSeGGw (Fig. 3J) ------- COMMENT: 037f5fa29955cd85 217 vihbG/Tep2MdKhsrX63AbzSeGGw (Fig. 3J) ------- COMMENT: 037f5fa29955cd85 218 vihbG/Tep2MdKhsrX63AbzSeGGw (Fig. 3J) ------- COMMENT: 037f5fa29955cd85 219 gmqogAnY3BQANKEbZmiyaveRfrg (Fig. 3E) ------- COMMENT: 037f5fa29955cd85 220 gmqogAnY3BQANKEbZmiyaveRfrg (Fig. 3E) ------- COMMENT: 037f5fa29955cd85 221 gmqogAnY3BQANKEbZmiyaveRfrg (Fig. 3E) ------- COMMENT: 037f5fa29955cd85 222 DnQNbBCyjr9h1gZ1Ef6ybZH1kkk (Fig. 3F) ------- COMMENT: 037f5fa29955cd85 223 DnQNbBCyjr9h1gZ1Ef6ybZH1kkk (Fig. 3F) ------- COMMENT: 037f5fa29955cd85 224 DnQNbBCyjr9h1gZ1Ef6ybZH1kkk (Fig. 3F) ------- COMMENT: 037f5fa29955cd85 225 dJB6QAubOSayNATA9sbJUiUH0oE (Fig. 3H) ------- COMMENT: 037f5fa29955cd85 226 dJB6QAubOSayNATA9sbJUiUH0oE (Fig. 3H) ------- COMMENT: 037f5fa29955cd85 227 dJB6QAubOSayNATA9sbJUiUH0oE (Fig. 3H) ------- COMMENT: 037f5fa29955cd85 228 RAQ9iCj6ucjGCQHQQosHYrKMC00 (Fig. 3I) ------- COMMENT: 037f5fa29955cd85 229 RAQ9iCj6ucjGCQHQQosHYrKMC00 (Fig. 3I) ------- COMMENT: 037f5fa29955cd85 230 RAQ9iCj6ucjGCQHQQosHYrKMC00 (Fig. 3I) ------- COMMENT: 037f5fa29955cd85 231 vihbG/Tep2MdKhsrX63AbzSeGGw (Fig. 3J) ------- COMMENT: 037f5fa29955cd85 232 vihbG/Tep2MdKhsrX63AbzSeGGw (Fig. 3J) ------- COMMENT: 037f5fa29955cd85 233 vihbG/Tep2MdKhsrX63AbzSeGGw (Fig. 3J) ------- COMMENT: 037f5fa29955cd85 234 gmqogAnY3BQANKEbZmiyaveRfrg (Fig. 3E) ------- COMMENT: 037f5fa29955cd85 235 gmqogAnY3BQANKEbZmiyaveRfrg (Fig. 3E) ------- COMMENT: 037f5fa29955cd85 236 gmqogAnY3BQANKEbZmiyaveRfrg (Fig. 3E) ------- COMMENT: 037f5fa29955cd85 237 dJB6QAubOSayNATA9sbJUiUH0oE (Fig. 3H) ------- COMMENT: 037f5fa29955cd85 238 dJB6QAubOSayNATA9sbJUiUH0oE (Fig. 3H) ------- COMMENT: 037f5fa29955cd85 239 dJB6QAubOSayNATA9sbJUiUH0oE (Fig. 3H) ------- COMMENT: 037f5fa29955cd85 240 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 037f5fa29955cd85 241 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 037f5fa29955cd85 242 MfhXrratyk9HEpPvYNKyPqJLEoI (Fig. 4E) ------- COMMENT: 037f5fa29955cd85 243 MfhXrratyk9HEpPvYNKyPqJLEoI (Fig. 4E) ------- COMMENT: 037f5fa29955cd85 244 MfhXrratyk9HEpPvYNKyPqJLEoI (Fig. 4E) ------- COMMENT: 037f5fa29955cd85 245 MfhXrratyk9HEpPvYNKyPqJLEoI (Fig. 4E) ------- COMMENT: 037f5fa29955cd85 246 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: 037f5fa29955cd85 247 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: 037f5fa29955cd85 248 kuRdbpnFrU9sjksgW9GQwEQ1424 (Fig. 5) ------- COMMENT: 037f5fa29955cd85 249 kuRdbpnFrU9sjksgW9GQwEQ1424 (Fig. 5) ------- COMMENT: 037f5fa29955cd85 250 lpY7AQKyqNozfDR1LvmNhtkakO4 (Fig. 6A-D) ------- COMMENT: 037f5fa29955cd85 251 Y8pqWYfUPfaXzWXbnDd55sL+ZA8 (Fig. 7) ------- COMMENT: 037f5fa29955cd85 252 Y8pqWYfUPfaXzWXbnDd55sL+ZA8 (Fig. 7) ------- COMMENT: 037f5fa29955cd85 253 Oe2njZK6TNdZY+IjnEHUZZWJXuw (Fig. 7B and G) ------- COMMENT: 037f5fa29955cd85 254 Oe2njZK6TNdZY+IjnEHUZZWJXuw (Fig. 7B and G) ------- COMMENT: 037f5fa29955cd85 255 Oe2njZK6TNdZY+IjnEHUZZWJXuw (Fig. 7B and G) ------- COMMENT: 037f5fa29955cd85 256 R0sjqLRQUfkUJeZM61SnbJdwnVs (Fig. 7C and H) ------- COMMENT: 037f5fa29955cd85 257 R0sjqLRQUfkUJeZM61SnbJdwnVs (Fig. 7C and H) ------- COMMENT: 037f5fa29955cd85 258 R0sjqLRQUfkUJeZM61SnbJdwnVs (Fig. 7C and H) ------- COMMENT: 037f5fa29955cd85 259 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 037f5fa29955cd85 260 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 037f5fa29955cd85 261 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 037f5fa29955cd85 262 /MgdeBHaT9ABix4/xBezuYbm/z8 (Fig. 7E) ------- COMMENT: 037f5fa29955cd85 263 /MgdeBHaT9ABix4/xBezuYbm/z8 (Fig. 7E) ------- COMMENT: 037f5fa29955cd85 264 /MgdeBHaT9ABix4/xBezuYbm/z8 (Fig. 7E) ------- COMMENT: 037f5fa29955cd85 265 p9PqWaxu79GLWhKfMyOCtz3437I (Fig. 7F) ------- COMMENT: 037f5fa29955cd85 266 p9PqWaxu79GLWhKfMyOCtz3437I (Fig. 7F) ------- COMMENT: 037f5fa29955cd85 267 p9PqWaxu79GLWhKfMyOCtz3437I (Fig. 7F) ------- COMMENT: 037f5fa29955cd85 268 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 037f5fa29955cd85 269 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 037f5fa29955cd85 270 bKUVPFcRiS0e2ZeZ4ffj9xKhmMg (Fig. 7H) ------- COMMENT: 037f5fa29955cd85 271 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 037f5fa29955cd85 272 bKUVPFcRiS0e2ZeZ4ffj9xKhmMg (Fig. 7H) ------- COMMENT: 037f5fa29955cd85 273 Oe2njZK6TNdZY+IjnEHUZZWJXuw (Fig. 7B and G) ------- COMMENT: 037f5fa29955cd85 274 Oe2njZK6TNdZY+IjnEHUZZWJXuw (Fig. 7B and G) ------- COMMENT: 037f5fa29955cd85 275 Oe2njZK6TNdZY+IjnEHUZZWJXuw (Fig. 7B and G) ------- COMMENT: 037f5fa29955cd85 276 R0sjqLRQUfkUJeZM61SnbJdwnVs (Fig. 7C and H) ------- COMMENT: 037f5fa29955cd85 277 R0sjqLRQUfkUJeZM61SnbJdwnVs (Fig. 7C and H) ------- COMMENT: 037f5fa29955cd85 278 R0sjqLRQUfkUJeZM61SnbJdwnVs (Fig. 7C and H) ------- COMMENT: 037f5fa29955cd85 279 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 037f5fa29955cd85 280 /MgdeBHaT9ABix4/xBezuYbm/z8 (Fig. 7E) ------- COMMENT: 037f5fa29955cd85 281 p9PqWaxu79GLWhKfMyOCtz3437I (Fig. 7F) ------- COMMENT: 037f5fa29955cd85 282 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 037f5fa29955cd85 283 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 037f5fa29955cd85 284 /MgdeBHaT9ABix4/xBezuYbm/z8 (Fig. 7E) ------- COMMENT: 037f5fa29955cd85 285 /MgdeBHaT9ABix4/xBezuYbm/z8 (Fig. 7E) ------- COMMENT: 037f5fa29955cd85 286 p9PqWaxu79GLWhKfMyOCtz3437I (Fig. 7F) ------- COMMENT: 037f5fa29955cd85 287 p9PqWaxu79GLWhKfMyOCtz3437I (Fig. 7F) ------- COMMENT: 037f5fa29955cd85 288 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 037f5fa29955cd85 289 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 037f5fa29955cd85 290 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 037f5fa29955cd85 291 bKUVPFcRiS0e2ZeZ4ffj9xKhmMg (Fig. 7H) ------- COMMENT: 037f5fa29955cd85 292 bKUVPFcRiS0e2ZeZ4ffj9xKhmMg (Fig. 7H) ------- COMMENT: 037f5fa29955cd85 293 Oe2njZK6TNdZY+IjnEHUZZWJXuw (Fig. 7B and G) ------- COMMENT: 037f5fa29955cd85 294 Oe2njZK6TNdZY+IjnEHUZZWJXuw (Fig. 7B and G) ------- COMMENT: 037f5fa29955cd85 295 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 037f5fa29955cd85 296 Oe2njZK6TNdZY+IjnEHUZZWJXuw (Fig. 7B and G) ------- COMMENT: 037f5fa29955cd85 297 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 037f5fa29955cd85 298 R0sjqLRQUfkUJeZM61SnbJdwnVs (Fig. 7C and H) ------- COMMENT: 037f5fa29955cd85 299 R0sjqLRQUfkUJeZM61SnbJdwnVs (Fig. 7C and H) ------- COMMENT: 037f5fa29955cd85 300 R0sjqLRQUfkUJeZM61SnbJdwnVs (Fig. 7C and H) ------- COMMENT: 037f5fa29955cd85 301 /MgdeBHaT9ABix4/xBezuYbm/z8 (Fig. 7E) ------- COMMENT: 037f5fa29955cd85 302 p9PqWaxu79GLWhKfMyOCtz3437I (Fig. 7F) ------- COMMENT: 037f5fa29955cd85 303 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 037f5fa29955cd85 304 /MgdeBHaT9ABix4/xBezuYbm/z8 (Fig. 7E) ------- COMMENT: 037f5fa29955cd85 305 /MgdeBHaT9ABix4/xBezuYbm/z8 (Fig. 7E) ------- COMMENT: 037f5fa29955cd85 306 p9PqWaxu79GLWhKfMyOCtz3437I (Fig. 7F) ------- COMMENT: 037f5fa29955cd85 307 p9PqWaxu79GLWhKfMyOCtz3437I (Fig. 7F) ------- COMMENT: 037f5fa29955cd85 308 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 037f5fa29955cd85 309 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 037f5fa29955cd85 310 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 037f5fa29955cd85 311 bKUVPFcRiS0e2ZeZ4ffj9xKhmMg (Fig. 7H) ------- COMMENT: 037f5fa29955cd85 312 bKUVPFcRiS0e2ZeZ4ffj9xKhmMg (Fig. 7H) ------- COMMENT: 037f5fa29955cd85 313 R0sjqLRQUfkUJeZM61SnbJdwnVs (Fig. 7C and H) ------- COMMENT: 037f5fa29955cd85 314 R0sjqLRQUfkUJeZM61SnbJdwnVs (Fig. 7C and H) ------- COMMENT: 037f5fa29955cd85 315 R0sjqLRQUfkUJeZM61SnbJdwnVs (Fig. 7C and H) ------- COMMENT: 037f5fa29955cd85 316 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 037f5fa29955cd85 317 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 037f5fa29955cd85 318 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 037f5fa29955cd85 319 /MgdeBHaT9ABix4/xBezuYbm/z8 (Fig. 7E) ------- COMMENT: 037f5fa29955cd85 320 /MgdeBHaT9ABix4/xBezuYbm/z8 (Fig. 7E) ------- COMMENT: 037f5fa29955cd85 321 p9PqWaxu79GLWhKfMyOCtz3437I (Fig. 7F) ------- COMMENT: 037f5fa29955cd85 322 p9PqWaxu79GLWhKfMyOCtz3437I (Fig. 7F) ------- COMMENT: 037f5fa29955cd85 323 Oe2njZK6TNdZY+IjnEHUZZWJXuw (Fig. 7B and G) ------- COMMENT: 037f5fa29955cd85 324 /MgdeBHaT9ABix4/xBezuYbm/z8 (Fig. 7E) ------- COMMENT: 037f5fa29955cd85 325 p9PqWaxu79GLWhKfMyOCtz3437I (Fig. 7F) ------- COMMENT: 037f5fa29955cd85 326 Oe2njZK6TNdZY+IjnEHUZZWJXuw (Fig. 7B and G) ------- COMMENT: 037f5fa29955cd85 327 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 037f5fa29955cd85 328 bKUVPFcRiS0e2ZeZ4ffj9xKhmMg (Fig. 7H) ------- COMMENT: 037f5fa29955cd85 329 bKUVPFcRiS0e2ZeZ4ffj9xKhmMg (Fig. 7H) ------- COMMENT: 037f5fa29955cd85 330 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 037f5fa29955cd85 331 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 037f5fa29955cd85 332 Oe2njZK6TNdZY+IjnEHUZZWJXuw (Fig. 7B and G) ------- COMMENT: 037f5fa29955cd85 333 Oe2njZK6TNdZY+IjnEHUZZWJXuw (Fig. 7B and G) ------- COMMENT: 037f5fa29955cd85 334 Oe2njZK6TNdZY+IjnEHUZZWJXuw (Fig. 7B and G) ------- COMMENT: 037f5fa29955cd85 335 R0sjqLRQUfkUJeZM61SnbJdwnVs (Fig. 7C and H) ------- COMMENT: 037f5fa29955cd85 336 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 037f5fa29955cd85 337 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 037f5fa29955cd85 338 /MgdeBHaT9ABix4/xBezuYbm/z8 (Fig. 7E) ------- COMMENT: 037f5fa29955cd85 339 p9PqWaxu79GLWhKfMyOCtz3437I (Fig. 7F) ------- COMMENT: 037f5fa29955cd85 340 p9PqWaxu79GLWhKfMyOCtz3437I (Fig. 7F) ------- COMMENT: 037f5fa29955cd85 341 p9PqWaxu79GLWhKfMyOCtz3437I (Fig. 7F) ------- COMMENT: 037f5fa29955cd85 342 Oe2njZK6TNdZY+IjnEHUZZWJXuw (Fig. 7B and G) ------- COMMENT: 037f5fa29955cd85 343 /MgdeBHaT9ABix4/xBezuYbm/z8 (Fig. 7E) ------- COMMENT: 037f5fa29955cd85 344 /MgdeBHaT9ABix4/xBezuYbm/z8 (Fig. 7E) ------- COMMENT: 037f5fa29955cd85 345 R0sjqLRQUfkUJeZM61SnbJdwnVs (Fig. 7C and H) ------- COMMENT: 037f5fa29955cd85 346 R0sjqLRQUfkUJeZM61SnbJdwnVs (Fig. 7C and H) ------- COMMENT: 037f5fa29955cd85 347 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 037f5fa29955cd85 348 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 037f5fa29955cd85 349 bKUVPFcRiS0e2ZeZ4ffj9xKhmMg (Fig. 7H) ------- COMMENT: 037f5fa29955cd85 350 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 037f5fa29955cd85 351 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 037f5fa29955cd85 352 bKUVPFcRiS0e2ZeZ4ffj9xKhmMg (Fig. 7H) ------- COMMENT: 037f5fa29955cd85 353 Oe2njZK6TNdZY+IjnEHUZZWJXuw (Fig. 7B and G) ------- COMMENT: 037f5fa29955cd85 354 Oe2njZK6TNdZY+IjnEHUZZWJXuw (Fig. 7B and G) ------- COMMENT: 037f5fa29955cd85 355 bKUVPFcRiS0e2ZeZ4ffj9xKhmMg (Fig. 7H) ------- COMMENT: 037f5fa29955cd85 356 R0sjqLRQUfkUJeZM61SnbJdwnVs (Fig. 7C and H) ------- COMMENT: 037f5fa29955cd85 357 R0sjqLRQUfkUJeZM61SnbJdwnVs (Fig. 7C and H) ------- COMMENT: 037f5fa29955cd85 358 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 037f5fa29955cd85 359 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 037f5fa29955cd85 360 /MgdeBHaT9ABix4/xBezuYbm/z8 (Fig. 7E) ------- COMMENT: 037f5fa29955cd85 361 /MgdeBHaT9ABix4/xBezuYbm/z8 (Fig. 7E) ------- COMMENT: 037f5fa29955cd85 362 p9PqWaxu79GLWhKfMyOCtz3437I (Fig. 7F) ------- COMMENT: 037f5fa29955cd85 363 p9PqWaxu79GLWhKfMyOCtz3437I (Fig. 7F) ------- COMMENT: 037f5fa29955cd85 364 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 037f5fa29955cd85 365 Oe2njZK6TNdZY+IjnEHUZZWJXuw (Fig. 7B and G) ------- COMMENT: 037f5fa29955cd85 366 R0sjqLRQUfkUJeZM61SnbJdwnVs (Fig. 7C and H) ------- COMMENT: 037f5fa29955cd85 367 p9PqWaxu79GLWhKfMyOCtz3437I (Fig. 7F) ------- COMMENT: 037f5fa29955cd85 368 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 037f5fa29955cd85 369 /MgdeBHaT9ABix4/xBezuYbm/z8 (Fig. 7E) ------- COMMENT: 037f5fa29955cd85 370 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 037f5fa29955cd85 371 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 037f5fa29955cd85 372 bKUVPFcRiS0e2ZeZ4ffj9xKhmMg (Fig. 7H) ------- COMMENT: 03a090dd69543b04 4 flmH2ykjdG63HPJk64RbSDH3O6A (comment: severity is variable, and segregates over successive generations (but not 2:2)) ------- COMMENT: 03a090dd69543b04 10 MGZwSTEPJwvW8jMJBfBFvkObiQg (comment: incomplete penetrance due to translational frameshifting) ------- COMMENT: 03a090dd69543b04 12 MGZwSTEPJwvW8jMJBfBFvkObiQg (comment: incomplete penetrance due to translational frameshifting) ------- COMMENT: 03a090dd69543b04 14 MGZwSTEPJwvW8jMJBfBFvkObiQg (comment: incomplete penetrance due to translational frameshifting) ------- COMMENT: 03a090dd69543b04 16 MGZwSTEPJwvW8jMJBfBFvkObiQg (comment: incomplete penetrance due to translational frameshifting) ------- COMMENT: 03a090dd69543b04 18 MGZwSTEPJwvW8jMJBfBFvkObiQg (comment: incomplete penetrance due to translational frameshifting) ------- COMMENT: 03a090dd69543b04 20 MGZwSTEPJwvW8jMJBfBFvkObiQg (comment: incomplete penetrance due to translational frameshifting) ------- COMMENT: 0401b77ec79d27fb 16 r739ksNf9pg5XSdgQ6l0BPTx0Bs (comment: inferred from decreased nda3 mRNA level) ------- COMMENT: 0401b77ec79d27fb 17 r739ksNf9pg5XSdgQ6l0BPTx0Bs (comment: inferred from decreased nda3 mRNA level) ------- COMMENT: 041a348f5a9e0ac6 1 qE8HgQvj+tUCYQ4pheG/xfxSGkc data not shown ------- COMMENT: 041a348f5a9e0ac6 2 m+RfalJxpr+UknmLEQXORwh3gNs Fig1b top R panel bottom L cdc25 is constituitively OP behind ADH promoter and nim1 is behind thiamine repressible promotor ------- COMMENT: 041a348f5a9e0ac6 3 YhIO1rSGlnN7vSjEtNik4Bm3SHI Fig1b top R panel, top cdc25 is constituitively OP behind ADH promoter and nim1 is behind thiamine repressible promotor ------- COMMENT: 041a348f5a9e0ac6 4 FkF3b+PY1Qvq4Dhud9bie0C5n6A Fig1b top R panel bottom R K41A is predicted to be catalytically inactive. cdc25 is constituitively OP behind ADH promoter and nim1 is behind thiamine repressible promotor ------- COMMENT: 041a348f5a9e0ac6 5 UzC6xMwr6xSS3iRNoOtPmeedriY Fig1b top R panel bottom R. K41A is predicted to be catalytically inactive ref 18. cdc25 is constituitively OP behind ADH promoter and nim1 is behind thiamine repressible promotor ------- COMMENT: 041a348f5a9e0ac6 6 77CQIvgRUjknXv9dPWWKipgLkkU Fig1b bottom R panel, top ------- COMMENT: 041a348f5a9e0ac6 7 m+RfalJxpr+UknmLEQXORwh3gNs Fig1b top R panel bottom L cdc25 is constituitively OP behind ADH promoter and nim1 is behind thiamine repressible promotor ------- COMMENT: 041a348f5a9e0ac6 8 YhIO1rSGlnN7vSjEtNik4Bm3SHI Fig1b top R panel, top cdc25 is constituitively OP behind ADH promoter and nim1 is behind thiamine repressible promotor ------- COMMENT: 041a348f5a9e0ac6 9 77CQIvgRUjknXv9dPWWKipgLkkU Fig1b bottom R panel, top ------- COMMENT: 041a348f5a9e0ac6 10 KIL+frS2SIRO5mLYCTa3/dWUeuE Fig1b bottom R panel, bottom L ------- COMMENT: 041a348f5a9e0ac6 11 z25cm5zMhCueRXRQV7duQl4/vG0 Fig1b bottom R panel, bottom L ------- COMMENT: 041a348f5a9e0ac6 12 XeILuuY6x6aUxYbdaX9dOmYZw74 Fig1b bottom R panel , bottom R K41A is predicted to be catalytically inactive. cdc25 is constituitively OP behind ADH promoter and nim1 is behind thiamine repressible promotor ------- COMMENT: 041a348f5a9e0ac6 13 RV5fDFm4yCtan7uofr+Ed5eBiss Fig1b bottom R panel , bottom R K41A is predicted to be catalytically inactive. cdc25 is constituitively OP behind ADH promoter and nim1 is behind thiamine repressible promotor ------- COMMENT: 041a348f5a9e0ac6 15 7Ak71IfU1hQEIpeObUm0Ry1icJE Fig2a lane 1 ------- COMMENT: 041a348f5a9e0ac6 16 Jq2MCyOaNedGdOmetX9VYfvDtWM Fig2a lane 3 ------- COMMENT: 041a348f5a9e0ac6 17 52udkA3VDjJBP64zqHWgMYYC/to Fig2b ------- COMMENT: 041a348f5a9e0ac6 18 KJJP7Uk0HIRxdtfoaw3bla+wWKA Fig2b ------- COMMENT: 041a348f5a9e0ac6 19 Fau9ghRrPQ8Q+OIguQNykF2wodw Fig 2b ------- COMMENT: 041a348f5a9e0ac6 20 tW5HLRlXXVcgUS/I/RsnrWL4gEA Fig2b 2x ------- COMMENT: 041a348f5a9e0ac6 23 M0DLWITojNp1A9ej3GMG4U0eZ48 (comment: proteins co produced in Sf9 cells causes mobility shift of wee1.) ------- COMMENT: 041a348f5a9e0ac6 24 ZgizLs6qA9JpF16d9sqPMznAkBs (comment: wee1 has reduced kinase activity) ------- COMMENT: 041a348f5a9e0ac6 25 4V8fx1N9wgZHvHVpsdc5q4qdTvM Fig4a,b together with data from fig 2,3 ------- COMMENT: 0431a5bb28c48745 2 /jr6ZXFrLr8X5xMRwoWMg9Zlrqg Figures 1A, S1A, S1B, 1D, S1E ------- COMMENT: 0431a5bb28c48745 3 icp1s7CrpUIulwG24amsXn/ZTOc Figures 1A, S1A, S1B, 1D, S1E ------- COMMENT: 0431a5bb28c48745 4 a6DsSWAMqX3yCbPJGqwJRTWIbIY Figures 2 ------- COMMENT: 0431a5bb28c48745 5 Rc+4l6UKh0jfxJ+oWhgymO0aN8w Figures 2C ------- COMMENT: 0431a5bb28c48745 6 TrppYAgvVrJbHPm+6MdjunkQ3iQ Figure 2D ------- COMMENT: 0431a5bb28c48745 8 oqwWHHadv/1vBv6LyHN6Sz/LQd4 Figure 2D (comment: CHECK abnormal cable clustering) ------- COMMENT: 0431a5bb28c48745 9 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: 0431a5bb28c48745 10 TrppYAgvVrJbHPm+6MdjunkQ3iQ Figure 2D ------- COMMENT: 0431a5bb28c48745 11 Ch9xPh53TqlaFcATkC9maKh5FS4 Figure Figure S4C ------- COMMENT: 0455bb4f542045e2 3 ffUr6QSjcgk+USSyOrSKcgjYqcY (comment: secretion of acid phosphatase) ------- COMMENT: 0455bb4f542045e2 5 ffUr6QSjcgk+USSyOrSKcgjYqcY (comment: secretion of acid phosphatase) ------- COMMENT: 04bb144c0a472009 1 jMve9yDU7FfzoHWGo/O8iKPeNfQ fig 1,7 ------- COMMENT: 04bb144c0a472009 2 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: 04bb144c0a472009 3 l9LgHl3O0MPocXNWH44GLxpFVKM fig 2,4 ------- COMMENT: 04bb144c0a472009 4 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 04bb144c0a472009 5 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 04bb144c0a472009 6 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: 04bb144c0a472009 8 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: 04bb144c0a472009 9 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: 04bb144c0a472009 11 1qhJWdmRrSZbATfIoK4vt3nRWE8 fig 8 (comment: CHECK increased calcinurin activity) ------- COMMENT: 04bb144c0a472009 16 4me+ag1mekppr4syEzguqoVICuM in agreement, the corresponding fractions 15–22 iso- lated from the fission yeast strain expressing GFP-tagged Cta4p were immuno-reactive with anti-GFP antibodies ------- COMMENT: 04c76a23c253368a 2 6yArwgWJCL7gY7ALm8kKkd1kG2k (comment: CHECK inhibited by methionine) ------- COMMENT: 04fc263cb7454d53 1 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 2 03ienHN103hzbhokIapL7vIAbk8 ------- COMMENT: 04fc263cb7454d53 3 03ienHN103hzbhokIapL7vIAbk8 ------- COMMENT: 04fc263cb7454d53 4 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 5 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 6 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 7 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 8 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 9 GZWIH6cT8cHNAZmxj3AbutRBhV4 ------- COMMENT: 04fc263cb7454d53 10 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 11 GZWIH6cT8cHNAZmxj3AbutRBhV4 ------- COMMENT: 04fc263cb7454d53 12 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 13 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 14 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 15 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 16 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 17 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 18 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 19 GZWIH6cT8cHNAZmxj3AbutRBhV4 ------- COMMENT: 04fc263cb7454d53 20 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 21 GZWIH6cT8cHNAZmxj3AbutRBhV4 ------- COMMENT: 04fc263cb7454d53 22 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 23 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 24 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 25 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 26 03ienHN103hzbhokIapL7vIAbk8 ------- COMMENT: 04fc263cb7454d53 27 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 28 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 29 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 30 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 31 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 32 03ienHN103hzbhokIapL7vIAbk8 ------- COMMENT: 04fc263cb7454d53 33 03ienHN103hzbhokIapL7vIAbk8 ------- COMMENT: 04fc263cb7454d53 34 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 35 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 36 GZWIH6cT8cHNAZmxj3AbutRBhV4 ------- COMMENT: 04fc263cb7454d53 38 03ienHN103hzbhokIapL7vIAbk8 ------- COMMENT: 04fc263cb7454d53 39 GZWIH6cT8cHNAZmxj3AbutRBhV4 ------- COMMENT: 04fc263cb7454d53 40 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 41 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 42 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 43 03ienHN103hzbhokIapL7vIAbk8 ------- COMMENT: 04fc263cb7454d53 44 GZWIH6cT8cHNAZmxj3AbutRBhV4 ------- COMMENT: 04fc263cb7454d53 45 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 46 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 47 JGud3m+N5nmGXFijhWJUL9lpGwE ------- COMMENT: 04fc263cb7454d53 48 6O/oiWz1p/PpNyaj8yAmhshJHRU ------- COMMENT: 04fc263cb7454d53 49 03ienHN103hzbhokIapL7vIAbk8 ------- COMMENT: 04fc263cb7454d53 50 03ienHN103hzbhokIapL7vIAbk8 ------- COMMENT: 04fc263cb7454d53 51 03ienHN103hzbhokIapL7vIAbk8 ------- COMMENT: 054ab9697f2d7ae0 2 +hJPSys+GXR8UbgFwGrYwnxayIg Yta4 competes with Dnm1 for binding Mdv1 and decreases the affinity of Dnm1 for GTP, and the overexpression of Yta4 delocalizes Dnm1 from mitochondria ------- COMMENT: 054ab9697f2d7ae0 3 M1rmB8G8hnHq9JRhV4+XY6ZSpIo Overexpression of Yta4 delocalizes Fis1 from mitochondria ------- COMMENT: 054ab9697f2d7ae0 4 jT04Up/Ei4jlkMowurwbH6YuVAE Overexpression of Yta4 delocalizes Mdv1 from mitochondria ------- COMMENT: 054ab9697f2d7ae0 5 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 054ab9697f2d7ae0 6 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 054ab9697f2d7ae0 7 lqoiTdHaKqTNqiP7tUbuexTRzYs Figure 1F and 1G Analysis by live-cell microscopy further showed that both fission and fusion frequencies increased significantly in cells lacking Yta4 ------- COMMENT: 054ab9697f2d7ae0 8 lqoiTdHaKqTNqiP7tUbuexTRzYs Figure 1F and 1G Analysis by live-cell microscopy further showed that both fission and fusion frequencies increased significantly in cells lacking Yta4 ------- COMMENT: 054ab9697f2d7ae0 9 lqoiTdHaKqTNqiP7tUbuexTRzYs Figure 1F and 1G Analysis by live-cell microscopy further showed that both fission and fusion frequencies increased significantly in cells lacking Yta4 ------- COMMENT: 054ab9697f2d7ae0 11 Nr+XgLoQR5V+FiGPzeXXqaAIgFI Quantification showed that the expression levels of endogenous Dnm1 were comparable in WT and yta4Δ cells (Fig 3B) ------- COMMENT: 054ab9697f2d7ae0 13 Hx1O/n1BrwR5+wvAPOTvLrmaVYU Yta4(EQ) impaired the for- mation of Dnm1 foci on mitochondria but unexpectedly caused mitochondria to aggregate (Figs 4D and S1C) ------- COMMENT: 054ab9697f2d7ae0 14 k8XIDS0vj8NzDGMozfVoWRMkWKY As shown in Figs 4D and S1C, overexpression of WT Yta4 impaired the formation of Dnm1 foci on mitochondria but did not change the tubular mito- chondrial morphology. ------- COMMENT: 054ab9697f2d7ae0 17 B90w51XmmJhcmvvdeUa+wAECMOc showed that the delocalized GFP-Fis1 appeared to be present in the cytoplasm of Yta4-overexpressing cells, while GFP-Fis1 in Yta4(AA)-overexpressing or Yta4 (EQ)-overexpressing cells was still present on mitochondria, which were clearly separated from the ER (S1D and S2 Figs). ------- COMMENT: 054ab9697f2d7ae0 18 B90w51XmmJhcmvvdeUa+wAECMOc showed that the delocalized GFP-Fis1 appeared to be present in the cytoplasm of Yta4-overexpressing cells, while GFP-Fis1 in Yta4(AA)-overexpressing or Yta4 (EQ)-overexpressing cells was still present on mitochondria, which were clearly separated from the ER (S1D and S2 Figs). ------- COMMENT: 054ab9697f2d7ae0 21 R6ROuCZjKQcv3f7BjKm5OytuFQU the expression of Yta4(WT), Yta4(AA), or Yta4(EQ) from the yta4 promoter restored Dnm1-associated mitochondrial fission to the WT level in yta4∆ cells (S3D Fig) ------- COMMENT: 054ab9697f2d7ae0 22 R6ROuCZjKQcv3f7BjKm5OytuFQU the expression of Yta4(WT), Yta4(AA), or Yta4(EQ) from the yta4 promoter restored Dnm1-associated mitochondrial fission to the WT level in yta4∆ cells (S3D Fig) ------- COMMENT: 054ab9697f2d7ae0 23 IfLSHnozXQi//Evg3H5yPH2mylI To reduce the complexity in analyzing Dnm1 GTPase activity, we performed colorimetric assays by using the assembly-defective version of Dnm1 (i.e., Dnm1(G380D), see S6A Fig). Interestingly, Dnm1(G380D) still exhibited a GTPase activ- ity but has a very low rate of GTP hydrolysis (Km = 85.00 μM, Vmax = 1.02 μM/min, versus the control Dnm1(WT): Km = 126.59 μM, Vmax = 4.82 μM/min) (S8 Fig). ------- COMMENT: 054ab9697f2d7ae0 24 IQTLAEFXKgwoWpgUcS9lVzIBZRs Hence, these results show the character- istic property of Yta4 in reducing the affinity of Dnm1 for GTP and in inhibiting Dnm1 assembly. ------- COMMENT: 0572af570458b258 1 8vmh9nO5IkIbaVHrwsihMRscwM8 Suppression either by missense mutation or deletion of pmr1 gene ------- COMMENT: 0572af570458b258 2 zW176gCdx7e5DLInXSBWmE0j3LE Morphological abnormality, manganese hyper-sensitivity, glucan accumulation, low-glucose sensitivity, loss of viability under nitrogen-starvation, and lipid accumulation are suppressed. ------- COMMENT: 0572af570458b258 3 MnJ0/uQ40kl4yXiaVMCCDTJVeEU Suppression by missense mutations of pga3 gene ------- COMMENT: 0572af570458b258 4 eILGo3dvg2dex3T0f4nVcc48smQ Morphological abnormality, manganese hyper-sensitivity, and glucan accumulation are suppressed. ------- COMMENT: 0572af570458b258 5 4GXqt+3wR29NKI7wJ4IEj9v5CpE Partial suppression of ts at 36℃ ------- COMMENT: 0572af570458b258 6 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 0572af570458b258 7 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 0572af570458b258 8 85KJuc3qZJV+obUU2zx1uJhAq18 Figure 5, ------- COMMENT: 0572af570458b258 9 wUJncOU82x0OG4BKvHrFU18mhEo Figure 5 (vw: changed to pear, descendent of spherical) ------- COMMENT: 0572af570458b258 10 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 0572af570458b258 23 hup5PgFzW5GksB0NE/mUSbxrOLs (comment: CHECK small viable) ------- COMMENT: 0572af570458b258 32 ny0fl4sRbLpnBiU3wPWyKdIC7Dc Fig. 2BC ------- COMMENT: 0572af570458b258 33 ny0fl4sRbLpnBiU3wPWyKdIC7Dc Fig. 2BC ------- COMMENT: 0572af570458b258 34 ny0fl4sRbLpnBiU3wPWyKdIC7Dc Fig. 2BC ------- COMMENT: 0572af570458b258 35 ny0fl4sRbLpnBiU3wPWyKdIC7Dc Fig. 2BC ------- COMMENT: 0572af570458b258 36 ny0fl4sRbLpnBiU3wPWyKdIC7Dc Fig. 2BC ------- COMMENT: 0572af570458b258 37 BPIrlYzNtwX2QPFlJb7spS+aWDY The resulting cwh43 pdt1Δ 201 double mutant partly recovered colony formation capacity at 36°C, compared to that of the 202 cwh43 single mutant (Fig. 2D). ------- COMMENT: 0572af570458b258 38 1J+96Oug87kKQCS6e0KeaZ0skGs Fig.3 ------- COMMENT: 0572af570458b258 39 1J+96Oug87kKQCS6e0KeaZ0skGs Fig.3 ------- COMMENT: 0572af570458b258 40 1J+96Oug87kKQCS6e0KeaZ0skGs Fig.3 ------- COMMENT: 0572af570458b258 41 1J+96Oug87kKQCS6e0KeaZ0skGs Fig.3 ------- COMMENT: 0572af570458b258 42 1J+96Oug87kKQCS6e0KeaZ0skGs Fig.3 ------- COMMENT: 0572af570458b258 43 1J+96Oug87kKQCS6e0KeaZ0skGs Fig.3 ------- COMMENT: 0572af570458b258 44 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 0572af570458b258 45 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 0572af570458b258 46 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 0572af570458b258 47 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 0572af570458b258 48 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 0572af570458b258 49 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 0572af570458b258 50 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 0572af570458b258 51 PAlSCsNJoWMQnn/zm8kYnyn7T7s figure 7 ------- COMMENT: 0572af570458b258 52 +bU6RNYWNIr7TU3nwnXP1yEziaA figure 7B small viable ------- COMMENT: 0572af570458b258 53 DvehpA0eRfPaPtCx2wU0rob3qnc figure 7B small, viable ------- COMMENT: 0572af570458b258 54 QL4xLcCxm6uRgsW87Av5uehYmAA figure 7B ------- COMMENT: 0572af570458b258 55 QL4xLcCxm6uRgsW87Av5uehYmAA figure 7B ------- COMMENT: 059994796bee610b 12 goEO9S3UHQnaYolcF2Vye6cN6UI (comment: dependent on F-actin, assayed using Latrunculin A) ------- COMMENT: 059994796bee610b 13 goEO9S3UHQnaYolcF2Vye6cN6UI (comment: dependent on F-actin, assayed using Latrunculin A) ------- COMMENT: 05cfc921ac6e7ee8 8 Zyte5xC5nislW9hs5+umgscCJmA (comment: Later stage of meiotic prophase, observed by co-localisation with Taz1) ------- COMMENT: 05cfc921ac6e7ee8 9 Zyte5xC5nislW9hs5+umgscCJmA (comment: Later stage of meiotic prophase, observed by co-localisation with Taz1) ------- COMMENT: 05cfc921ac6e7ee8 23 zYHy/xvALE1rSd4dZkkid6ts9Ds S10 E and F ------- COMMENT: 05cfc921ac6e7ee8 28 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 05cfc921ac6e7ee8 29 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 05cfc921ac6e7ee8 34 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: 05ebdc773cdd0a9d 8 uFRDd6VjQJNcJwEctw06EGDpK8s fig 4e In order to test whether the Gcn2 kinase induces autophagy through phosphorylation of eIF2a, we constructed a strain that expresses eIF2a with its phosphorylation site Ser52 substituted by ala- nine (eIF2a-S52A). ------- COMMENT: 05ebdc773cdd0a9d 12 z4AZ2cY/TDWWMm7JQj3O9Rv2Epc Moreover, S. pombe cells lacking Gcn3 (Figure 4D) or Fil1 (Figure 4F) displayed autophagy defects during leucine starvation. ------- COMMENT: 05ebdc773cdd0a9d 13 5qLLT0xG9AVhGw7x36WymS1tPNA Indeed, we observed that, after leucine starvation, the Fil1 protein increased, which was dependent on Gcn2 and the phosphorylation of eIF2a (Fig- ure 4—figure supplement 1H). ------- COMMENT: 05ebdc773cdd0a9d 16 4KPnWNatUrNnAHdXz93PiYfGRXw In the gcn2D tsc2D double-mutant cells starved of nitrogen, autophagy was severely reduced and delayed, in comparison to those in the wild-type and individual single-mutant cells (Figure 6A). ------- COMMENT: 05ebdc773cdd0a9d 19 43tUXZi8iQIUdQ25cey9pPr1MrM The autophagy defect in the gcn2D iml1D double mutant was even more severe than that in the gcn2D tsc2D double mutant (Figure 6B,C), implying that GATOR1 and Gcn2 are two main contributors to the nitrogen starvation-induced autophagy. ------- COMMENT: 05ebdc773cdd0a9d 39 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 05ebdc773cdd0a9d 40 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 05ebdc773cdd0a9d 41 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 05ebdc773cdd0a9d 42 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 05ebdc773cdd0a9d 43 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 05ebdc773cdd0a9d 44 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 05ebdc773cdd0a9d 45 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 05ebdc773cdd0a9d 46 dEIItLnXB4wS0eAszxbqoDvc7e4 Figure 1, Supp 1 ------- COMMENT: 05ebdc773cdd0a9d 47 dEIItLnXB4wS0eAszxbqoDvc7e4 Figure 1, Supp 1 ------- COMMENT: 05ebdc773cdd0a9d 48 dEIItLnXB4wS0eAszxbqoDvc7e4 Figure 1, Supp 1 ------- COMMENT: 05ebdc773cdd0a9d 49 dEIItLnXB4wS0eAszxbqoDvc7e4 Figure 1, Supp 1 ------- COMMENT: 05ebdc773cdd0a9d 50 dEIItLnXB4wS0eAszxbqoDvc7e4 Figure 1, Supp 1 ------- COMMENT: 05ebdc773cdd0a9d 51 dEIItLnXB4wS0eAszxbqoDvc7e4 Figure 1, Supp 1 ------- COMMENT: 05ebdc773cdd0a9d 53 dEIItLnXB4wS0eAszxbqoDvc7e4 Figure 1, Supp 1 ------- COMMENT: 05ebdc773cdd0a9d 54 15DJ/AK4e1AX1ZFN6j6WeBfeW4o (comment: vw I edited to make the response the extension to GCN2 mediated signalling, but I hope to improve these GO terms.) ------- COMMENT: 05ebdc773cdd0a9d 56 G9qHrUPmPLhw+81txUpvS1b3sII Gcn2-dependent induction of autoph- agy was also observed in cells treated by 3-amino-1,2,4-triazole (3-AT) or methionine sulfoximine (MSX), inhibitors of histidine and glutamine biosynthesis, respectively (Figure 4—figure supplement 1D,E). ------- COMMENT: 05ebdc773cdd0a9d 57 48iWxcuvVKy3KchuTRHm/zanIsk Autophagy in response to leucine starvation was abrogated by the gcn1D, but not gcn20D, mutation (Figure 4D), ------- COMMENT: 05ebdc773cdd0a9d 59 kXHSpkKfPlIKY6Yt0BP1dJQ2iW8 We found that autophagy after leucine starvation was severely impaired in cells lacking Cpc2 and in those overexpressing a fission yeast ortholog of Yih1/IMPACT (Figure 4—figure supplement 1G), confirming the essential role of the Gcn2 activity in autophagy induction upon amino acid starvation. ------- COMMENT: 05ebdc773cdd0a9d 64 SLJ/0KkhPRmqKTHEMBTA/34HlSI fig 4 a Though much less than that in wild-type cells, autophagy was still detectable in the tsc2D iml1D double mutant, indicating that, in addition to the GATOR1 and TSC complexes, there must be an additional mechanism to attenuate TORC1 upon nitrogen starvation for autophagy induction. ------- COMMENT: 05ebdc773cdd0a9d 66 zVx51YJWYe/8scxB60/8JcHsb0I fig4a ------- COMMENT: 05ebdc773cdd0a9d 69 tN/dyDxxphb/QcUkcoQZMj5eZUA Figure 2a ------- COMMENT: 05ebdc773cdd0a9d 70 tN/dyDxxphb/QcUkcoQZMj5eZUA Figure 2a ------- COMMENT: 05ebdc773cdd0a9d 71 tN/dyDxxphb/QcUkcoQZMj5eZUA Figure 2a ------- COMMENT: 05ebdc773cdd0a9d 72 tN/dyDxxphb/QcUkcoQZMj5eZUA Figure 2a ------- COMMENT: 05ebdc773cdd0a9d 73 tN/dyDxxphb/QcUkcoQZMj5eZUA Figure 2a ------- COMMENT: 05ebdc773cdd0a9d 74 tN/dyDxxphb/QcUkcoQZMj5eZUA Figure 2a ------- COMMENT: 05ebdc773cdd0a9d 75 tN/dyDxxphb/QcUkcoQZMj5eZUA Figure 2a ------- COMMENT: 05ebdc773cdd0a9d 76 tN/dyDxxphb/QcUkcoQZMj5eZUA Figure 2a ------- COMMENT: 05ebdc773cdd0a9d 77 tN/dyDxxphb/QcUkcoQZMj5eZUA Figure 2a ------- COMMENT: 05ebdc773cdd0a9d 78 cIb3Za6y+h8N9WcDRGQnYaHA5XQ Figure 2b ------- COMMENT: 05ebdc773cdd0a9d 79 O3lO+RAFVhVoveV7ci5226/X/TQ Figure 2b (comment: any1 rescues) ------- COMMENT: 05ebdc773cdd0a9d 80 4nWsXwz4uxdxdLjcQlFcrlBvwAg Figure 2b (comment: any1 rescues) ------- COMMENT: 05ebdc773cdd0a9d 81 YCUZjIiNm8h6fLOLGO5TmFiopLQ Figure 2e (comment: sea3 rescued by gtr1 GDP-locked) ------- COMMENT: 05ebdc773cdd0a9d 82 CTK2JYFQcw6WQ3euKF034wzJ8do Figure 2f ------- COMMENT: 05ebdc773cdd0a9d 83 YCUZjIiNm8h6fLOLGO5TmFiopLQ Figure 2e (comment:sea3 rescued by gtr1 GDP-locked) ------- COMMENT: 05ebdc773cdd0a9d 84 PrJo5nMYg0Ri219V0X7/UiSFQm8 Figure 2g ------- COMMENT: 05ebdc773cdd0a9d 85 WUwCLE6ExuA8DGOTqNz6bgVAnLc On the other hand, Iml1 and Sea3 were co-immunoprecipitated even in the absence of Seh1, Sea2, and Sea4 (Figure 3E), implying that Sea3 directly binds to GATOR1 and anchors the other GATOR2 components to GATOR1. ------- COMMENT: 05ebdc773cdd0a9d 86 MtvcVXXN/5v3D2pdKbKj491iC7s The binding of Sea3 to GATOR1 is dependent on the integrity of the GATOR1 complex, and the absence of any one of the GATOR1 subunits disrupted the Sea3–GATOR1 association (Figure 3F,G and H). ------- COMMENT: 05ebdc773cdd0a9d 87 MtvcVXXN/5v3D2pdKbKj491iC7s The binding of Sea3 to GATOR1 is dependent on the integrity of the GATOR1 complex, and the absence of any one of the GATOR1 subunits disrupted the Sea3–GATOR1 association (Figure 3F,G and H). ------- COMMENT: 05ebdc773cdd0a9d 88 MtvcVXXN/5v3D2pdKbKj491iC7s The binding of Sea3 to GATOR1 is dependent on the integrity of the GATOR1 complex, and the absence of any one of the GATOR1 subunits disrupted the Sea3–GATOR1 association (Figure 3F,G and H). ------- COMMENT: 05ebdc773cdd0a9d 89 Q+dxBZN4xDJ4DoQCJhvk6QuJVks Consis- tently, in the absence of intact GATOR1, the vacuolar localization of Sea3 (Figure 1E) was lost and the protein diffused throughout the cytosol (Figure 3I, Figure 3—figure supplement 1A), ------- COMMENT: 05ebdc773cdd0a9d 90 0vpulz0V5v4fE5CC5lS/WTEKV34 Furthermore, consi tent with the essential role of Sea3 in the interaction between GATOR1 and the other GATOR2 sub- units, the vacuolar localization of Sea2, Sea4, and Seh1 ( was abrogated in the sea3D background (Figure 3—figure supplement 1C–E). ------- COMMENT: 05ebdc773cdd0a9d 91 5FoX1b7pbXiy4pk8xNvp+ivNN/o (Figure 3 figure supplement 1C–E). ------- COMMENT: 05ebdc773cdd0a9d 92 5FoX1b7pbXiy4pk8xNvp+ivNN/o (Figure 3 figure supplement 1C–E). ------- COMMENT: 05ebdc773cdd0a9d 93 ccc1Zz5IqtLyay4pqez+gMQFeYI In the case of the sea3D mutant, Iml1, Npr2, and Npr3 were all detectable on vacuoles (Figure 3—figure supplement 2E,F and G), ------- COMMENT: 05ebdc773cdd0a9d 94 wBjtUnJEnE8QG01hzKlqQ9QekMc (Figure 3 figure supplement 2E,F and G), ------- COMMENT: 05ebdc773cdd0a9d 95 wBjtUnJEnE8QG01hzKlqQ9QekMc (Figure 3 figure supplement 2E,F and G), ------- COMMENT: 05ebdc773cdd0a9d 96 BsgnU3gt43qDpMCf+1x0CxzgiWI On the other hand, immuno- precipitation of the GATOR1 subunit Iml1 found that the physical interaction between GATOR1 and the Gtr1 GTPase was reduced in the sea3D mutant (Figure 3M). ------- COMMENT: 05ebdc773cdd0a9d 97 r8hPxNAv220a8UdA2ewYKhzSN28 (Figure 3—figure supplement 3A), indicating that Arg854 of S. pombe Iml1 is not essential for the GATOR1 function. ------- COMMENT: 05ebdc773cdd0a9d 98 dOYaa20Z0bYRdyKmJ7DD2SC4eyw Recently, another conserved arginine residue in mammalian GATOR1, Arg78 of the Nprl2 subunit,was proposed to serve as an arginine finger that promotes GTP hydrolysis by RagA/B (Shen et al.,2019, Figure 3—figure supplement 3B). To assess the role of the equivalent residue in the S.pombe GATOR1, Arg98 in Npr2 was substituted with alanine to construct an npr2R98A mutantstrain. The mutant cells exhibited a compromised growth phenotype that was rescued by rapamycinor the gtr1SN mutation, an indicative of compromised GAP activity of GATOR1 (Figure 3—figuresupplement 3C). Though the npr2R98A phenotype was not as severe as that of the npr2 nullmutant, these observations are in line with the model that the conserved Arg residue in Npr2, butnot the one in Iml1, acts as an arginine finger of GATOR1 also in fission yeast. ------- COMMENT: 05ebdc773cdd0a9d 99 dOYaa20Z0bYRdyKmJ7DD2SC4eyw Recently, another conserved arginine residue in mammalian GATOR1, Arg78 of the Nprl2 subunit,was proposed to serve as an arginine finger that promotes GTP hydrolysis by RagA/B (Shen et al.,2019, Figure 3—figure supplement 3B). To assess the role of the equivalent residue in the S.pombe GATOR1, Arg98 in Npr2 was substituted with alanine to construct an npr2R98A mutantstrain. The mutant cells exhibited a compromised growth phenotype that was rescued by rapamycinor the gtr1SN mutation, an indicative of compromised GAP activity of GATOR1 (Figure 3—figuresupplement 3C). Though the npr2R98A phenotype was not as severe as that of the npr2 nullmutant, these observations are in line with the model that the conserved Arg residue in Npr2, butnot the one in Iml1, acts as an arginine finger of GATOR1 also in fission yeast. ------- COMMENT: 05ebdc773cdd0a9d 100 MPycZGnO4Y5xFBPDNunh1ltN7+o However, no GFP accumulation was detected in gcn2D cells under leucine starvation (Figure 4C), demonstrating that the autophagy induced by leucine starvation is dependent on the Gcn2 kinase. ------- COMMENT: 05ebdc773cdd0a9d 101 71KzD+kwS2J/YY0WqJfCllfGbLA (comment: CONDITION Arginine starvation) Similarly, in cells of arginine auxotrophy, Gcn2-dependent autophagy was detectable after incubation in the growth medium without arginine (Figure 4—figure supplement 1C). ------- COMMENT: 05ebdc773cdd0a9d 106 dW4ysSs9ysuXETVsN1ZxffXh9dE In contrast, Psk1 remained phosphorylated even after the starvation in the gcn2D, eIF2a-S52A, and fil1D mutant strains (Figure 5A), suggesting that TORC1 inactivation in leucine-starved cells is medi- ated by the Gcn2-eIF2a-Fil1 pathway. ------- COMMENT: 05ebdc773cdd0a9d 109 kxFVrxH/Sm+mXO10U8UErbimHts Moreover, the autophagy defect of the gcn2D mutant was complemented by TORC1 inactivation by the TORC1 inhibitors, rapamycin and caffeine (Figure 5B). ------- COMMENT: 05ebdc773cdd0a9d 110 CZizmjf/PMJhA3aYldChg/fAnTg In the gnc2D iml1D tsc2D triple mutant, a trace of released GFP was detected only after 14 hr of nitrogen starvation (Figure 6C), while autophagy takes place within 2 hr in wild-type cells after the starvation (Figure 4B). ------- COMMENT: 05fb9cabc575e97e 1 89sFO05cOQptNdCDlfqZfDFRPm4 Table S1, Supplementary Data ------- COMMENT: 05fb9cabc575e97e 2 89sFO05cOQptNdCDlfqZfDFRPm4 Table S1, Supplementary Data ------- COMMENT: 05fb9cabc575e97e 3 89sFO05cOQptNdCDlfqZfDFRPm4 Table S1, Supplementary Data ------- COMMENT: 0639ddd6e2750840 1 UyD4EFSHOur8vhbdQxyn+FTsGQM Based on these results we speculate a protective role of Bsd1 in response to tunicamycin-induced ER stress. ------- COMMENT: 0639ddd6e2750840 2 wKfL+TBhqY1ElpQ776hGTy7OPGM The confocal microscopic analysis using GFP tagged Bsd1 suggests a typical endoplasmic reticulum mem- branous localization of Bsd1-GFP (Fig. 2b, left panel) [32]. Interestingly, in the presence of tunicamycin, the Bsd1-GFP localization was more pronounced (Fig. 2b, right pane ------- COMMENT: 0639ddd6e2750840 3 DkGgk68qXcSLvo27qq2B5xWeSvE The spotting assay analysis revealed that the bsd1Δ cells exhibit sensitivity against tunicamycin (Fig. 1c) ------- COMMENT: 0639ddd6e2750840 4 4gjHcajHxJ83R1GZcLOEEUiHvJQ The epistatic interaction analysis between bsd1Δ and ire1Δ cells revealed a relative increase in tunicamycin sensitivity in bsd1Δ ire1Δ double mutant as compared to each single mutant (Fig. 5a). ------- COMMENT: 0639ddd6e2750840 6 HJlzuCAGcfNGrFuU30KwBXzM8v4 ------- COMMENT: 0639ddd6e2750840 7 2wxoJTbRopwp0QV6YMdBqu+GWLM ------- COMMENT: 0639ddd6e2750840 8 qUEe2ZREsHqHbSgVn7cOholwL94 Furthermore, we checked the growth of S. pombe cells in the presence of 2-deoxy-glucose and observed the concen- tration-dependent sensitivity of bsd1delta ------- COMMENT: 0639ddd6e2750840 9 1RcIwocX7XAz8hEeGAXOOUGwnr0 Fig. 3 Tunicamycin exposure leads to apoptotic cell death and aberrant nuclear morphology. ------- COMMENT: 0639ddd6e2750840 10 ODyNypYHnr77QW3t3dowfvgRJwI Interestingly, unlike wild type cells, the bsd1Δ cells showed punctate FM4-64 staining after tunicamycin expo- sure (Fig. 4), reminiscent of endosome like intermediate compartment previously has been reported in vps28 mutant cells that exhibit a defect in protein sorting process in bud- ding yeast [34]. ------- COMMENT: 067e21ef8aa97ffd 1 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 067e21ef8aa97ffd 2 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: 067e21ef8aa97ffd 3 N3IrWkN6FUtHfTt6JCaaWP4L+8c fig 2 A ------- COMMENT: 067e21ef8aa97ffd 5 zKxgyRAsYgWL7f58cuMZpRjtV+I fig 2 B ------- COMMENT: 067e21ef8aa97ffd 6 zKxgyRAsYgWL7f58cuMZpRjtV+I fig 2 B ------- COMMENT: 067e21ef8aa97ffd 7 zKxgyRAsYgWL7f58cuMZpRjtV+I fig 2 B ------- COMMENT: 067e21ef8aa97ffd 8 cWU1gxrBhSdpW25jqWFTdTZ9qBA fig 3A ------- COMMENT: 067e21ef8aa97ffd 9 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: 067e21ef8aa97ffd 10 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: 067e21ef8aa97ffd 11 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: 067e21ef8aa97ffd 12 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: 067e21ef8aa97ffd 13 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: 067e21ef8aa97ffd 14 yn5/s6S+P83NU/sZm1yJpcjeWbk fig 3D ------- COMMENT: 067e21ef8aa97ffd 15 pwcwASlWQtRBBoormzj5gHYObdE fig 4A ------- COMMENT: 067e21ef8aa97ffd 16 //QleQcD3kkWGdAJo2oN7LSuYAA fig 4B ------- COMMENT: 067e21ef8aa97ffd 17 JPOuJ4EqAJ/Z46aEtjJ0cjqNLec fig 4B,C ------- COMMENT: 067e21ef8aa97ffd 18 BprhfysZecSHnDRVGx+GjRUIYco fig 4 D ------- COMMENT: 067e21ef8aa97ffd 19 BprhfysZecSHnDRVGx+GjRUIYco fig 4 D ------- COMMENT: 067e21ef8aa97ffd 22 JPOuJ4EqAJ/Z46aEtjJ0cjqNLec fig 4B,C ------- COMMENT: 067e21ef8aa97ffd 23 lVvMgkFNoEp4aURnzoIpcy7J//0 fig 5A ------- COMMENT: 067e21ef8aa97ffd 24 5Pc+GJjJVhHm1h5Cm22Rhx2qUks fig 5B ------- COMMENT: 067e21ef8aa97ffd 25 sJQr2wKA4JZdSpWnZ3qr4VqJ9Hw fig 7B ------- COMMENT: 067e21ef8aa97ffd 26 Mf87d+JaK4YguHC6nZu1eLfH3Ck fig 7C ------- COMMENT: 067e21ef8aa97ffd 27 q7HgqESKFwjxqQAGO1gMZ7R0MGw fig 8A ------- COMMENT: 067e21ef8aa97ffd 28 lfvonS/vLq2B4Dj7Zhz2RIn7cFQ fig 8B ------- COMMENT: 067e21ef8aa97ffd 29 lfvonS/vLq2B4Dj7Zhz2RIn7cFQ fig 8B ------- COMMENT: 067e21ef8aa97ffd 30 o1g9guFzuQCQwf86Xp3GwbkRtfc fig 8C ------- COMMENT: 067e21ef8aa97ffd 31 o1g9guFzuQCQwf86Xp3GwbkRtfc fig 8C ------- COMMENT: 067e21ef8aa97ffd 33 yn5/s6S+P83NU/sZm1yJpcjeWbk fig 3D ------- COMMENT: 06a0bcdb548ec92c 1 XXyNRw+KKzK8g24Jl3vAF5Fsrwg Fig. 8 and text ------- COMMENT: 06a0bcdb548ec92c 22 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 06a0bcdb548ec92c 23 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 06a0bcdb548ec92c 24 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 06a0bcdb548ec92c 25 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 06a0bcdb548ec92c 26 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 06a0bcdb548ec92c 27 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 06a0bcdb548ec92c 28 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 06a0bcdb548ec92c 29 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 06a0bcdb548ec92c 30 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 06a0bcdb548ec92c 31 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 06a0bcdb548ec92c 32 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 06a0bcdb548ec92c 33 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 06a0bcdb548ec92c 36 XXN1yCM4x/0Ph65KVvRJIRc75es Fig. 12 (Note how the levels are the same as when the pyrophosphatase is inactivated in the full-length protein) ------- COMMENT: 06a0bcdb548ec92c 37 QF8Bu1uK2rNFGvQ+GaN0DNatJ3g Fig. 8 & 12 ------- COMMENT: 06a0bcdb548ec92c 38 24OZrOgdlqsMzgBAIO26tu76ih0 Fig. 12 ------- COMMENT: 06a0bcdb548ec92c 39 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: 06a0bcdb548ec92c 40 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: 06a0bcdb548ec92c 41 24OZrOgdlqsMzgBAIO26tu76ih0 Fig. 12 ------- COMMENT: 06a0bcdb548ec92c 42 24OZrOgdlqsMzgBAIO26tu76ih0 Fig. 12 ------- COMMENT: 06a0bcdb548ec92c 43 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: 06a0bcdb548ec92c 44 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: 06a0bcdb548ec92c 45 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 06a0bcdb548ec92c 46 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 06c34b4476712d4c 1 Uuy6CVDUskOG05hl8dIJ2s4hvqk (comment: just to get the allele details of -P in the database) ------- COMMENT: 06c4d8895a9200b4 3 Ii2h2hHmat1lfmCXWbLYa+/lyIg (comment: activated_by CHEBI:29108 | activated_by CHEBI:18420 | inhibited_by CHEBI:16761) ------- COMMENT: 06d77d7ac3f77bbc 2 PFFLsiLpIobkOPEzKy4ep0RqQR8 (comment: "poison" product of shorter alternative transcript;assayed by expressing S.k. ortholog in S.p.) ------- COMMENT: 06d77d7ac3f77bbc 3 +cXTN2fi4CjoaipbAGHEvrxoH0I (comment: "antidote" product of longer alternative transcript; assayed by expressing S.k. ortholog in S.p.) ------- COMMENT: 06e82eaa6f509bdb 38 7ZTDa32Z3cK7Yyq2bqs6cs8ovCk Fig. 2b ------- COMMENT: 06e82eaa6f509bdb 39 7ZTDa32Z3cK7Yyq2bqs6cs8ovCk Fig. 2b ------- COMMENT: 06e82eaa6f509bdb 40 7ZTDa32Z3cK7Yyq2bqs6cs8ovCk Fig. 2b ------- COMMENT: 06e82eaa6f509bdb 41 lBe2xqZDFhZWW5J9ZH5+r6cEsTI Fig. 2b (comment: control) ------- COMMENT: 06e82eaa6f509bdb 42 FN7sNjOyW9dVqPZhH0csPmInUDA Main text Table S1 ------- COMMENT: 06e82eaa6f509bdb 43 FN7sNjOyW9dVqPZhH0csPmInUDA Main text Table S1 ------- COMMENT: 06e82eaa6f509bdb 44 FN7sNjOyW9dVqPZhH0csPmInUDA Main text Table S1 ------- COMMENT: 06e82eaa6f509bdb 45 FN7sNjOyW9dVqPZhH0csPmInUDA Main text Table S1 ------- COMMENT: 06e82eaa6f509bdb 46 FN7sNjOyW9dVqPZhH0csPmInUDA Main text Table S1 ------- COMMENT: 06e82eaa6f509bdb 47 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 06e82eaa6f509bdb 48 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 06e82eaa6f509bdb 49 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 073a79f341c124df 1 lWOWlkLu7c7B+KroQvHoy2ljXBw RNA level expressed from the ribosomal protein-coding gene, rpl30-2, was increased by 4.5-fold in the absence of Pab2 (Lemay et al., 2010). To independently validate this result, we compared rpl30-2 mRNA levels between wild-type and pab2D strains by northern analysis and confirmed a 3-fold increase of rpl30-2 mRNA in pab2 null cells (Figure 1A, lanes 1 and 2). The use of an intron-specific probe confirmed that the slower- migrating rpl30-2 transcript is the unspliced pre-mRNA (Fig- ure 1A, lane 4). ------- COMMENT: 073a79f341c124df 2 jpeWaHygVJo1+Sy1gqM6yDiFmeY specific to one of the two rpl30 paralogs, as the expression of rpl30-1 was unchanged in pab2D cells (Figure 1A). ------- COMMENT: 073a79f341c124df 3 p49LgvnQ3C+HH1O3Xbqv9Ph1IXg Interestingly, the intronless rpl30-2 construct resulted in increased levels of mRNA relative to the intron-containing construct (Figure 1B, lanes 1 and 4); yet, mRNA levels were not increased in the pab2D strain when rpl30-2 was expressed from the intronless construct (Figure 1B, lanes 4 and 5, and Figure 1C). ------- COMMENT: 073a79f341c124df 4 zx5UqCAp+R3id17iuyJBwt3nS5o Northern blot analysis of RNA prepared from the rrp6D strain revealed robust upregu- lation of rpl30-2 mRNA and pre-mRNA, 5- and 18-fold, respec- tively (Figure 2A, lane 7, and Figures 2B and 2C), ------- COMMENT: 073a79f341c124df 5 WM4eqKIdaygh6BX8NcqiX2yql44 whereas rpl30-2 mRNA and pre-mRNA were upregulated 1.5- and 3.5- fold, respectively, using RNA from the dis3 mutant (Figure 2A, lane 5, and Figures 2B and 2C). ------- COMMENT: 073a79f341c124df 6 okl0fXw6tKEXLijIqpKWtBd0YFw rpl30-2 mRNA and pre-mRNA were higher in the pab2D dis3-54 double-mutant strain compared to the single dis3-54 mutant (Figure 2A, compare lanes 5 and 6, and Figures 2B and 2C). ------- COMMENT: 073a79f341c124df 7 aTnGm8p5yFi8NnYQC6cUKt/9mhY In contrast, no cumulative increase in the levels of rpl30-2 tran- scripts was observed in the pab2D rrp6D double-mutant strain relative to the single rrp6D strain (Figure 2A, lanes 7 and 8, and Figures 2B and 2C). ------- COMMENT: 073a79f341c124df 8 g6LiO8YV3bWP9i0U2MBaXThiCsg primarily mediated in the nucleus, as the deletion of ski7, which encodes a cytosolic-specific exosome cofactor, did not perturb rpl30-2 expression (Figure 2A, lane 9, and Figures 2B and 2C). ------- COMMENT: 073a79f341c124df 9 +rwQWIQSuCn49gaBpJ87U9Q7XWY Analysis of RNA from cid14D cells showed normal levels of rpl30-2 pre-mRNA and mRNA (Figure 2A, lane 3, and Figures 2B and 2C) ------- COMMENT: 073a79f341c124df 10 6iWCQIzzhQs5cdJ6uCRGNVafSeM However, the deletion of cid14 in the pab2D strain increased the levels of rpl30-2 mRNA and pre-mRNA compared to the single pab2D strain (Figure 2A, compare lanes 2 and 4). ------- COMMENT: 073a79f341c124df 11 xNBuswJg5YQqJmKOEqDpJGDC1i4 Similar results were obtained with a conditional strain in which the genomic copy of mtr4 is expressed from the thiamine-sensi- tive nmt1+ promoter: depletion of Mtr4 did not affect rpl30-2 pre- mRNA and mRNA levels (Figure 2D, compare lanes 3 and 7) ------- COMMENT: 073a79f341c124df 12 5f2wgEJalYmg0660qxWAVE4zMsc however, depletion of Mtr4 in the pab2D strain increased the levels of rpl30-2 mRNA and pre-mRNA compared to the single pab2D strain (Figure 2D, compare lanes 4 and 8). ------- COMMENT: 073a79f341c124df 13 x6MepN/IqfzznLWy6+yNEjidcdI Consistent with this, rpl30-2 expression levels were unaffected in the rrp6D strain when rpl30-2 was ex- pressed from the intronless construct (Figure 1B, lanes 4 and 6), similar to results using the pab2D mutant (Figure 1B, lane 5). ------- COMMENT: 073a79f341c124df 14 Sr7jtks8JbqBhccIcgUfAQpV3e0 In contrast, the expression of nonpolyadenylated rpl30-2 from the ribozyme construct was largely insensitive to Pab2- and Rrp6-dependent degradation (Figure 3C, lanes 4–6). ------- COMMENT: 073a79f341c124df 15 JeFDPZrWM6m5IA39L9DumR38DM4 the ability of Pab2 to bind a poly(A) substrate was completely lost after the substitution of a conserved phenylalanine (F75R) residue within the RNA recognition motif of Pab2 (Figures S3C and S3D). ------- COMMENT: 073a79f341c124df 16 PhXZxX4sd9SKL86BJenw/gN4rPM Similarly, a Pab2 variant in which the 11 arginine resi- dues within the arginine/glycine-rich domain were substituted to alanine (R-to-A) showed no poly(A) binding (Figure S3) ------- COMMENT: 073a79f341c124df 17 Ho73z4ndxpYwgQo7a3lPFNWH8QU Impor- tantly, Pab2 variants F75R and R-to-A did not rescue the increased levels of rpl30-2 transcripts observed in the pab2D strain (Figure 3E, lanes 2–4), although the two Pab2 variants defective in poly(A) binding were expressed at levels similar to wild-type Pab2 (Figure 3F). ------- COMMENT: 073a79f341c124df 18 Ho73z4ndxpYwgQo7a3lPFNWH8QU Impor- tantly, Pab2 variants F75R and R-to-A did not rescue the increased levels of rpl30-2 transcripts observed in the pab2D strain (Figure 3E, lanes 2–4), although the two Pab2 variants defective in poly(A) binding were expressed at levels similar to wild-type Pab2 (Figure 3F). ------- COMMENT: 073a79f341c124df 19 wDLWVFTWowE4OhiBkdlR/RWwbSQ we found a 2-fold increase in rpl30-2 mRNA levels after shifting cells growing at 25C to 37C (Figure 4A, lanes 1 and 2, and Figure 4B). ------- COMMENT: 073a79f341c124df 20 7m4b/n84jPiFiF2XGvnpa5kpJeA RNA level expressed from the ribosomal protein-coding gene, rpl30-2, was increased by 4.5-fold in the absence of Pab2 (Lemay et al., 2010). To independently validate this result, we compared rpl30-2 mRNA levels between wild-type and pab2D strains by northern analysis and confirmed a 3-fold incemperature-dependent upregulation of rpl30-2 required Pab2, as it was not observed in a pab2D strain that is defective in pre-mRNA decay (Figure 4A, lanes 3 and 4, and Figure 4B). ------- COMMENT: 073a79f341c124df 21 x4i44JUMMcNR+aY9NNXxq6D7FJA If negative control of pre-mRNA decay is mainly responsible for the upregulation of rpl30-2 after heat shock, we predicted that rpl30-2 expression from the intronless construct, which is insensitive to Pab2/Rrp6-mediated pre-mRNA decay (Figures 1B and 1C), would not respond to heat stress. Accord- ingly, a wild-type strain that expressed the intronless version of rpl30-2 showed no increase in mRNA levels after heat shock (Figure 4C, lanes 1 and 2, and Figure 4D). ------- COMMENT: 073a79f341c124df 27 k3m1YuDOTr4eUZmoKUUktKGsaKo Strikingly, we noted a 6-fold decrease in the percentage of unspliced rpl30-2 transcript in the absence of Rpl30-1 relative to the wild-type (Figure S5A) ------- COMMENT: 073a79f341c124df 29 X7+xcm7vNaoC1/LrCTKqhENHfMo In agreement with the direct role of Rpl30-1 in the control of rpl30-2 expression, excess Rpl30-1 re- sulted in decreased levels of rpl30-2 mRNA (Figure 6D, lane 4). ------- COMMENT: 077db45134eec113 1 AZ7ZAKRN3RlMm4R6Jo48gzOXfL0 (comment: SMC5 AA 1-225+837-1065) ------- COMMENT: 077db45134eec113 2 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 077db45134eec113 3 SMJ+dJ0jvSMhIyIBGFXZrYZkDm0 fig 1 b ------- COMMENT: 077db45134eec113 4 SMJ+dJ0jvSMhIyIBGFXZrYZkDm0 fig 1 b ------- COMMENT: 077db45134eec113 5 XPnM32shLX68Eo2rR66Yrm1uKgQ fig 1 c ------- COMMENT: 077db45134eec113 6 RdPxKy6TA/oocuhfrRm1ufsavvc fig 1 d ------- COMMENT: 077db45134eec113 7 iRc67pN5dJpwz7PrdHUbbP1yjfo fig 1 F ------- COMMENT: 077db45134eec113 8 clCNCEkZF+H3EMt4ISWux784FJU fig 1 f ------- COMMENT: 077db45134eec113 9 clCNCEkZF+H3EMt4ISWux784FJU fig 1 f ------- COMMENT: 077db45134eec113 10 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 077db45134eec113 11 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 077db45134eec113 12 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 077db45134eec113 13 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 077db45134eec113 14 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 077db45134eec113 15 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 077db45134eec113 16 cPraZhmoDEUsT8bzKtHH46w/yWE Fig 1c lane 9 ------- COMMENT: 077db45134eec113 17 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 077db45134eec113 18 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 077db45134eec113 19 BlASlsc3jjDacGfaT4iZTB9S9YI fig 5 A ------- COMMENT: 077db45134eec113 20 e+l0InXr8+vVi3R3h3XdYa5HYgY fig 5 C ------- COMMENT: 077db45134eec113 21 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 077db45134eec113 22 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 077db45134eec113 23 iWOfUtKG5yBOgHJg1VEiQiRUfmo fig 6 A, lane 9 ------- COMMENT: 077db45134eec113 24 TR+/dbjwvh9kmnxQtDpxGvT4jdo fig 6 B, lane 3 ------- COMMENT: 077db45134eec113 25 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 077db45134eec113 26 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 077db45134eec113 27 FYECrm9X44ZLFe/aSTin4KQchvM fig 7 A ------- COMMENT: 077db45134eec113 28 FYECrm9X44ZLFe/aSTin4KQchvM fig 7 A ------- COMMENT: 077db45134eec113 29 v5AEUd6YKKd+CXxhtdOFUdayL0k fig 6 B, lane 9 ------- COMMENT: 07bc527001c082d8 20 WkqZpRe1lFx/Y5CHZE8qDsAFAmA (comment: same as rid1-1 alone) ------- COMMENT: 07bc527001c082d8 21 YPv7cKDBy6SkfVDDQw3tWz5BQ0Y (comment: same as rid2-1 alone) ------- COMMENT: 081f29c16c8596ad 6 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 081f29c16c8596ad 7 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 081f29c16c8596ad 8 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 081f29c16c8596ad 9 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 081f29c16c8596ad 10 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 081f29c16c8596ad 11 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 081f29c16c8596ad 12 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 081f29c16c8596ad 13 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 081f29c16c8596ad 14 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 081f29c16c8596ad 15 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 081f29c16c8596ad 16 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 081f29c16c8596ad 17 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 081f29c16c8596ad 18 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 081f29c16c8596ad 19 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 081f29c16c8596ad 20 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 081f29c16c8596ad 21 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 081f29c16c8596ad 22 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 081f29c16c8596ad 23 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 081f29c16c8596ad 24 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 081f29c16c8596ad 25 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 083a547f8abebb96 1 WIQobKVmOSwF4pnw3zyHiiYk2Lk fig1a ------- COMMENT: 083a547f8abebb96 3 mqb7asrVw9BGMWNezfrbNuewGPs fig1 a ------- COMMENT: 083a547f8abebb96 4 dEbRWmdPG9Szv+BeCtj0l6DfExk fig1 2 ------- COMMENT: 083a547f8abebb96 5 dEbRWmdPG9Szv+BeCtj0l6DfExk fig1 2 ------- COMMENT: 083a547f8abebb96 6 d441T9J3TIJYRB/C5eXCmFmi7l4 fig1 B ------- COMMENT: 083a547f8abebb96 7 d441T9J3TIJYRB/C5eXCmFmi7l4 fig1 B ------- COMMENT: 083a547f8abebb96 8 nnuWhrFU7jaQ2CsXT4A21I2oLbM fig 3a ------- COMMENT: 083a547f8abebb96 9 eFuSSdCYiwhcJaxY5DqQ8V4i1cg igure 3, B and D ------- COMMENT: 083a547f8abebb96 10 1T4fXTv82lkmVksJT6tf9vz2YfE figure 3, B and D ------- COMMENT: 083a547f8abebb96 11 mqb7asrVw9BGMWNezfrbNuewGPs fig1 a ------- COMMENT: 083a547f8abebb96 12 mqb7asrVw9BGMWNezfrbNuewGPs fig1 a ------- COMMENT: 083a547f8abebb96 13 9BSDRu77gCcpiKi803iu7V3zM+k table 2 ------- COMMENT: 083a547f8abebb96 14 9BSDRu77gCcpiKi803iu7V3zM+k table 2 ------- COMMENT: 083a547f8abebb96 15 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 083a547f8abebb96 16 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 083a547f8abebb96 17 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 083a547f8abebb96 18 MTyEGP4m3aCw3xo9QYvULYrAg5k fig 6 B ------- COMMENT: 083a547f8abebb96 19 MTyEGP4m3aCw3xo9QYvULYrAg5k fig 6 B ------- COMMENT: 083c3fcd870879b0 1 O/0XZUlhq3Lzoz0d7cYsFw28fws (comment: localization independent of Ago1) ------- COMMENT: 083c3fcd870879b0 4 O/0XZUlhq3Lzoz0d7cYsFw28fws (comment: localization independent of Ago1) ------- COMMENT: 083c3fcd870879b0 20 Ww1ssLhHt0pnQTYFgkSUzKEQahE (comment: localization independent of Ago1) ------- COMMENT: 083c3fcd870879b0 21 O/0XZUlhq3Lzoz0d7cYsFw28fws (comment: localization independent of Ago1) ------- COMMENT: 08562810fce65760 1 ynyHxYBw2XsgrSwmE2TCB2N1dH8 (comment: Rad3 dependent) ------- COMMENT: 08562810fce65760 2 ynyHxYBw2XsgrSwmE2TCB2N1dH8 (comment: Rad3 dependent) ------- COMMENT: 08562810fce65760 5 ynyHxYBw2XsgrSwmE2TCB2N1dH8 (comment: Rad3 dependent) ------- COMMENT: 08562810fce65760 6 ynyHxYBw2XsgrSwmE2TCB2N1dH8 (comment: Rad3 dependent) ------- COMMENT: 08562810fce65760 7 ynyHxYBw2XsgrSwmE2TCB2N1dH8 (comment: Rad3 dependent) ------- COMMENT: 08562810fce65760 8 ynyHxYBw2XsgrSwmE2TCB2N1dH8 (comment: Rad3 dependent) ------- COMMENT: 08562810fce65760 9 HKIoMdIbb8WFb3OE6ZKQ7sC2cIM (comment: Phosphorylated at Tf2-type retrotransposons and wtf elements during S-phase)| (comment: Rad3 dependent) ------- COMMENT: 08562810fce65760 10 eoZZcBU8wHMKeTAh6bkFOaT3+jA (comment: Phosphorylated at Tf2-type retrotransposons and wtf elements during S-phase) ------- COMMENT: 08562810fce65760 33 HnxvlYyg5FOErZ0VhNxmDe19Brg ------- COMMENT: 08562810fce65760 120 MAcAE3U8PgXXtqWG3vwVpp6Sv0M (comment: Phosphorylated at mating type locus during S-phase), (comment: Rad3 dependent) ------- COMMENT: 08562810fce65760 121 2k/AOFTQyW/hqWEVQQVsSIL2vww (comment: Phosphorylated at mating type locus during S-phase) (comment: Rad3 dependent) ------- COMMENT: 08562810fce65760 122 Tk9pY/Fdcz4bmeHg/6v9R4PLPv0 (comment: Phosphorylated at centromeres during S-phase) (comment: Rad3 dependent) ------- COMMENT: 08562810fce65760 123 Tk9pY/Fdcz4bmeHg/6v9R4PLPv0 (comment: Phosphorylated at centromeres during S-phase) (comment: Rad3 dependent) ------- COMMENT: 08562810fce65760 124 h1DUBY4TdDbH9M2xNVybCmHrroM (comment: Phosphorylated at Tf2-type retrotransposons and wtf elements during S-phase) ------- COMMENT: 089bad866432ef28 2 zEWGHo8sc3EDTKHuqKYKlsQSmHs (comment: change to: twin horsetail nucleus) ------- COMMENT: 089bad866432ef28 3 iHcWz0sP+UNtpiA5ePRqq1MSuGg (comment: Change to: Nuclear congression without nuclear fusion) ------- COMMENT: 089bad866432ef28 11 gKZaQSgJkG7rWjreF0MDH7L1pU4 (comment: akr1Δ affecting tht1) ------- COMMENT: 089bad866432ef28 18 V+4CEfwyCgcSe9WpYU1RBsExOjY ------- COMMENT: 089bad866432ef28 20 zEWGHo8sc3EDTKHuqKYKlsQSmHs (comment: change to: twin horsetail nucleus) ------- COMMENT: 089bad866432ef28 21 iHcWz0sP+UNtpiA5ePRqq1MSuGg (comment: Change to: Nuclear congression without nuclear fusion) ------- COMMENT: 089bad866432ef28 24 zEWGHo8sc3EDTKHuqKYKlsQSmHs (comment: change to: twin horsetail nucleus) ------- COMMENT: 089bad866432ef28 25 iHcWz0sP+UNtpiA5ePRqq1MSuGg (comment: Change to: Nuclear congression without nuclear fusion) ------- COMMENT: 089bad866432ef28 28 aDEkakSRWkS/r3AQKVxL28imS4M (comment: Lower levels in the akr1 mutant) ------- COMMENT: 089bad866432ef28 29 aDEkakSRWkS/r3AQKVxL28imS4M (comment: Lower levels in the akr1 mutant) ------- COMMENT: 089bad866432ef28 30 FG+y1sdonaARz7JtnwbOxw3BFN0 (comment: palmitoylation of tht1D is reduced by akr1D) ------- COMMENT: 089bad866432ef28 31 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 08c090c708be03da 20 vkXOeM+oSLBmE7oY8DqzIuTC5r0 (comment: from the catenated plasmid experiment and failure to separate sisters) ------- COMMENT: 08caaf0d4ea1eb4b 2 KqkskUAzTM24d+TbNe2IYimP7AQ (comment: knocked out in diploid. Can't tell if it vegetative or spore?) ------- COMMENT: 08caaf0d4ea1eb4b 4 KqkskUAzTM24d+TbNe2IYimP7AQ (comment: knocked out in diploid. Can't tell if it vegetative or spore?) ------- COMMENT: 08caaf0d4ea1eb4b 17 gOWde/FLRLEjcs5yWACP8Hl9N6c (27% of spores produce viable colonies) ------- COMMENT: 08cc3df57adda022 5 Chuua0tqkfujQK5J6PM8W4jmKfQ (Fig. 3a) ------- COMMENT: 08cc3df57adda022 6 Chuua0tqkfujQK5J6PM8W4jmKfQ (Fig. 3a) ------- COMMENT: 08cc3df57adda022 7 Chuua0tqkfujQK5J6PM8W4jmKfQ (Fig. 3a) ------- COMMENT: 08cc3df57adda022 8 Chuua0tqkfujQK5J6PM8W4jmKfQ (Fig. 3a) ------- COMMENT: 08cc3df57adda022 9 Chuua0tqkfujQK5J6PM8W4jmKfQ (Fig. 3a) ------- COMMENT: 08cc3df57adda022 10 Chuua0tqkfujQK5J6PM8W4jmKfQ (Fig. 3a) ------- COMMENT: 08cc3df57adda022 11 Chuua0tqkfujQK5J6PM8W4jmKfQ (Fig. 3a) ------- COMMENT: 08cc3df57adda022 12 Chuua0tqkfujQK5J6PM8W4jmKfQ (Fig. 3a) ------- COMMENT: 08cc3df57adda022 13 PEijj9MIrNSBSecC4MlXqO1v4Zk (Fig. 3) ------- COMMENT: 08d41b8c55934bfd 2 jdzt20Pg7pYAZQgIkrJhyLFYAkU Fig. 5A,B ------- COMMENT: 08d41b8c55934bfd 3 jdzt20Pg7pYAZQgIkrJhyLFYAkU Fig. 5A,B ------- COMMENT: 08d41b8c55934bfd 4 jdzt20Pg7pYAZQgIkrJhyLFYAkU Fig. 5A,B ------- COMMENT: 08d41b8c55934bfd 5 0sLfXawgU33yZdR+mfKqyO8ixPs (comment: endocytosis restricted to cell end) ------- COMMENT: 08d41b8c55934bfd 6 0sLfXawgU33yZdR+mfKqyO8ixPs (comment: endocytosis restricted to cell end) ------- COMMENT: 08d41b8c55934bfd 7 0sLfXawgU33yZdR+mfKqyO8ixPs (comment: endocytosis restricted to cell end) ------- COMMENT: 08d41b8c55934bfd 8 0sLfXawgU33yZdR+mfKqyO8ixPs (comment: endocytosis restricted to cell end) ------- COMMENT: 08fa7aa06148221f 1 rFARi2n7M1b/hPPue0NBUvytIeg (comment: Erh1 localizes with Mmi1 both during mitotic cell cycle and meiosis) ------- COMMENT: 08fa7aa06148221f 23 w/cyeGVrx4CTdb7MmQFobmUlJa8 (comment: some up some down) ------- COMMENT: 08fa7aa06148221f 24 w/cyeGVrx4CTdb7MmQFobmUlJa8 (comment: some up some down) ------- COMMENT: 08fa7aa06148221f 59 7kqELljH0ZyVw7/HSjNNPIRhi64 (comment: author statement) ------- COMMENT: 0917f87a0b7a8795 1 V06/5VMqx4HRFC47hVSg0YQEx5o A spot assay showed that the cdc14-E2 allele was comparable in its temperature sensitivity to cdc14-118 (Figure 1C). ------- COMMENT: 0917f87a0b7a8795 2 iwArKePZ/vBlNNO79ZlcvDgkf18 While cdc14-118 cells showed the classic sin phenotype of multinucleation and cell elongation at the non-permissive temperature, cdc14-E2 cells arrested uniformly at a very late stage of septation and frequently lysed (Figure 1B). ------- COMMENT: 0917f87a0b7a8795 3 V06/5VMqx4HRFC47hVSg0YQEx5o A spot assay showed that the cdc14-E2 allele was comparable in its temperature sensitivity to cdc14-118 (Figure 1C). ------- COMMENT: 0917f87a0b7a8795 4 iwArKePZ/vBlNNO79ZlcvDgkf18 While cdc14-118 cells showed the classic sin phenotype of multinucleation and cell elongation at the non-permissive temperature, cdc14-E2 cells arrested uniformly at a very late stage of septation and frequently lysed (Figure 1B). ------- COMMENT: 0917f87a0b7a8795 5 yjHOZ6UY1MRsSeQwxphAmrIY/fk All temperature-sensitive alleles grew less than wildtype at 36°C with the cdc16-C1 allele showing the greatest temperature-sensitivity (Figure 1I). ------- COMMENT: 0917f87a0b7a8795 6 mFRr+/9rYjAcl30yk/PKINpxCXs The phenotypes of the three mutants were comparable. At 25 ̊C, the percent of septated cells was 17-20 with none showing more than one septa and at 36 ̊C, all cells arrested with multiple septa and one or two nuclei (Figure 1H). ------- COMMENT: 0917f87a0b7a8795 7 yjHOZ6UY1MRsSeQwxphAmrIY/fk All temperature-sensitive alleles grew less than wildtype at 36°C with the cdc16-C1 allele showing the greatest temperature-sensitivity (Figure 1I). ------- COMMENT: 0917f87a0b7a8795 8 y9iBS6knE6hI9Xihppd6VCpd7F4 (figure 1H) ------- COMMENT: 0917f87a0b7a8795 9 yjHOZ6UY1MRsSeQwxphAmrIY/fk All temperature-sensitive alleles grew less than wildtype at 36°C with the cdc16-C1 allele showing the greatest temperature-sensitivity (Figure 1I). ------- COMMENT: 0917f87a0b7a8795 10 NoCUphpbIVKxBTEAsEKu6mO/iNc (Figure 1H) ------- COMMENT: 0917f87a0b7a8795 11 dBgSp6hbVf5okmwYMz4zC7UvEck A spot assay revealed that sid1-L2 had an intermediate restrictive temperature compared to sid1-125 and sid1-239 (Figure 1F). ------- COMMENT: 0917f87a0b7a8795 12 BhREhUkx6AvEfwoTnUEA1uufM/M Nuclei and septa staining revealed predominantly a boomerang-shape phenotype that was often accompanied by cell lysis at septation (Figure 1E). ------- COMMENT: 0917f87a0b7a8795 14 zKuIeg92X5u1PlQqcPHbwMw1t9Y (Figure 1E) ------- COMMENT: 0927ffeae1602f26 5 920Q9A21F/+7Ic0Fevc8Buw9Caw Second, Dcp1 stabilizes the fold of the Dcp2 RD, especially around the split active site, as revealed by hydrogen deuterium exchange rates (SI Appendix, Fig. S13). Finally, Edc1 enforces the active orientation in Dcp2 (Fig. 1 B–D) through specific interaction between its YAG activation motif and Dcp2. ------ COMMENT: 0927ffeae1602f26 6 920Q9A21F/+7Ic0Fevc8Buw9Caw Second, Dcp1 stabilizes the fold of the Dcp2 RD, especially around the split active site, as revealed by hydrogen deuterium exchange rates (SI Appendix, Fig. S13). Finally, Edc1 enforces the active orientation in Dcp2 (Fig. 1 B–D) through specific interaction between its YAG activation motif and Dcp2. ------- COMMENT: 0985d9c7ca7f7a05 1 BRKFMwPd5C0IcLrX8E6uq+dnySQ Further analysis revealed that the levels of intron-retaining cox1 and cob1 tran- scripts were increased in 􏰀ppr10 cells in the intron-containing background (Fig. 1A–C) ------- COMMENT: 0985d9c7ca7f7a05 7 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 0985d9c7ca7f7a05 9 7nbsvMi98FcFh2zJIclZPAKqWSI The results showed that in the intronless background, the cox1 and cob1 mRNAs were detected at similar levels in WT[􏰀i] and 􏰀ppr10[􏰀i] cells (Fig. 1E–G ------- COMMENT: 09a0a50064982418 4 +L85eHuBsHBjInEG1Vnii/Fruxs (comment: exists during veg growth & glucose starv & HU stress) ------- COMMENT: 09b5fcafa2826d4a 13 BthzppA4H5Q+GoCWwthb67+Ityw (comment: abnormal cleavage furrow disc formation) fig 3 ------- COMMENT: 09b5fcafa2826d4a 14 BthzppA4H5Q+GoCWwthb67+Ityw (comment: abnormal cleavage furrow disc formation) fig 3 ------- COMMENT: 09b5fcafa2826d4a 15 BthzppA4H5Q+GoCWwthb67+Ityw (comment: abnormal cleavage furrow disc formation) fig 3 ------- COMMENT: 09b5fcafa2826d4a 16 BthzppA4H5Q+GoCWwthb67+Ityw (comment: abnormal cleavage furrow disc formation) fig 3 ------- COMMENT: 09b5fcafa2826d4a 17 BthzppA4H5Q+GoCWwthb67+Ityw (comment: abnormal cleavage furrow disc formation) fig 3 ------- COMMENT: 09b96530a975cab0 8 Wb6V+PucAeD9x4MbtzJ1ufT1FlY (comment: heterochromatin silencing defect is enhanced in the double mutant) ------- COMMENT: 09ee8fea7eb8ca88 1 qOyhLUXJ+PuGTrR0DT6JtA5g4Us A direct Ppc89-Sid4 interaction was confirmed in vitro 19 with recombinantly produced GST-Ppc89(545-687) and MBP-Sid4(546-660) (Figure 20 2F). ------- COMMENT: 09ee8fea7eb8ca88 2 0UPz3bu10bZyJG9N1J8CS3O4BfI We found 22 that ppc89-3 was synthetically lethal with sid4-SA1 and synthetically sick with spg1-106, 23 mob1-R4, and plo1-25 (Figure S1B,C). ------- COMMENT: 09ee8fea7eb8ca88 3 o7ji6qkU2skPva/HK1r/WfKiBGc ppc89-4 was also synthetically lethal with sid4- 12 Downloaded from https://academic.oup.com/g3journal/advance-article/doi/10.1093/g3journal/jkae249/7848890 by guest on 01 November 2024 1 SA1 (Figure S1C) ------- COMMENT: 09ee8fea7eb8ca88 4 0UPz3bu10bZyJG9N1J8CS3O4BfI We found 22 that ppc89-3 was synthetically lethal with sid4-SA1 and synthetically sick with spg1-106, 23 mob1-R4, and plo1-25 (Figure S1B,C). ------- COMMENT: 09ee8fea7eb8ca88 5 0UPz3bu10bZyJG9N1J8CS3O4BfI We found 22 that ppc89-3 was synthetically lethal with sid4-SA1 and synthetically sick with spg1-106, 23 mob1-R4, and plo1-25 (Figure S1B,C). ------- COMMENT: 09ee8fea7eb8ca88 6 0UPz3bu10bZyJG9N1J8CS3O4BfI We found 22 that ppc89-3 was synthetically lethal with sid4-SA1 and synthetically sick with spg1-106, 23 mob1-R4, and plo1-25 (Figure S1B,C). ------- COMMENT: 09ee8fea7eb8ca88 7 rhUO9hrA/R+qgVYqH9O1DBouY9M ppc89-2, ppc89-3 and ppc89-4 grow 19 similarly to wildtype at 25°C but do not form colonies at 36°C (Figure 1B ------- COMMENT: 09ee8fea7eb8ca88 8 rhUO9hrA/R+qgVYqH9O1DBouY9M ppc89-2, ppc89-3 and ppc89-4 grow 19 similarly to wildtype at 25°C but do not form colonies at 36°C (Figure 1B ------- COMMENT: 09ee8fea7eb8ca88 9 rhUO9hrA/R+qgVYqH9O1DBouY9M ppc89-2, ppc89-3 and ppc89-4 grow 19 similarly to wildtype at 25°C but do not form colonies at 36°C (Figure 1B ------- COMMENT: 09ee8fea7eb8ca88 10 ubrPXkTlJG7VLHwj0mreh1ZhHAY ppc89(1-707)-mNG cells were 7 viable but temperature-sensitive (Figure 3C). ------- COMMENT: 09ee8fea7eb8ca88 11 UgIEKttFEwD6qDSrPbOg2w02Lws ppc89-L756P,I770V was 3 temperature-sensitive and accumulated cells with one nucleus and a septum (Figure 4 6B-D), reminiscent of what we observed in ppc89-4 cells ------- COMMENT: 09ee8fea7eb8ca88 12 P/eXzcobM7P3V6QXl0P/7/IOH24 At 36°C, ppc89-2 and ppc89-3 displayed an increased proportion of binucleate cells with “kissing” nuclei in which nuclei return to the cell center after a failed cytokiness as well as cell lysis (Figure 1C,D) ------- COMMENT: 09ee8fea7eb8ca88 13 P/eXzcobM7P3V6QXl0P/7/IOH24 At 36°C, ppc89-2 and ppc89-3 displayed an increased proportion of binucleate cells with “kissing” nuclei in which nuclei return to the cell center after a failed cytokiness as well as cell lysis (Figure 1C,D) ------- COMMENT: 09ee8fea7eb8ca88 14 G8LmrKBhydA0BeIAlfw0MpgiUBA . A 2 portion of ppc89-4 cells at 36°C showed an additional abnormal phenotype – cells with 3 a septum but only one nucleus (Figure 1C,D). ------- COMMENT: 09ee8fea7eb8ca88 15 9EFg91mjBGoKpCB/tqAGhwVz7SY Moreover, we found that 5 ppc89(1-707)-mNG cells accumulated mononucleated, septated cells and anucleate cell 6 compartments (Figure 6C,D) as well as multiple Ppc89 foci (Figure 6E and S4B). ------- COMMENT: 09ee8fea7eb8ca88 17 GalbiuuLODQUBSRwtR7gzXM4ZNg ppc89-L756P,I770V was 3 temperature-sensitive and accumulated cells with one nucleus and a septum (Figure 4 6B-D), reminiscent of what we observed in ppc89-4 cells| Moreover, we found that 5 ppc89(1-707)-mNG cells accumulated mononucleated, septated cells and anucleate cell 6 compartments (Figure 6C,D) as well as multiple Ppc89 foci (Figure 6E and S4B). ------- COMMENT: 09ee8fea7eb8ca88 18 P/eXzcobM7P3V6QXl0P/7/IOH24 At 36°C, ppc89-2 and ppc89-3 displayed an increased proportion of binucleate cells with “kissing” nuclei in which nuclei return to the cell center after a failed cytokiness as well as cell lysis (Figure 1C,D) ------- COMMENT: 09ee8fea7eb8ca88 19 8oN9RxPbEn/6a3jS2RD3cZGT9Pc At 36°C, ppc89-2 and ppc89-3 displayed an increased proportion of binucleate cells with “kissing” nuclei in which nuclei return to the cell center after a failed cytokinesis as well as cell lysis (Figure 1C,D) ------- COMMENT: 09ee8fea7eb8ca88 20 oclkMPSZsdyY2LTcGa9E+YDXmbc Ppc89-3-mNG localized to the 10 SPB at both permissive and restrictive temperatures similarly to Ppc89-mNG (Figures 11 1E, F and S1A). ------- COMMENT: 09ee8fea7eb8ca88 21 P+73qGJggBPqsRhd3A2ebmyEL68 In contrast, Ppc89-4-mNG showed a ~50% reduction in SPB 12 fluorescence intensity at both temperatures compared to wildtype (Figures 1E, F and 13 S1A). ------- COMMENT: 09ee8fea7eb8ca88 22 j3y4knZBiU5GF7SLjkcPWbPaBrA In ppc89-3 cells at the restrictive temperature, we noticed an increased proportion 14 of cells displaying 2 or 4 Ppc89-3-mNG and Sad1-mCherry foci, indicative of cytokinesis 15 failure (Figure 1G,H). In contrast, ppc89-4 cells displayed 3, 4, or ≥5 Ppc89-4-mNG and 16 Sad1-mCherry foci (Figure 1G,H), indicating that the integrity of the SPB as a whole is 17 disrupted in ppc89-4 cells whereas it appears to remain intact in ppc89-3 cells. ------- COMMENT: 09ee8fea7eb8ca88 24 /CkjmsRvlMAcsFBLLIlzOgIWENc Interestingly we found that the additional foci of Ppc89-4, 11 observed in 169/280 cells that progressed through mitosis during the movies, always 12 formed during anaphase by splitting off from one of the two SPBs (Figure 7A). By 13 determining the intensity of the two SPBs before and after foci appeared, we confirmed 14 that the fragments originated from one SPB because the fluorescence intensity of that 15 SPB always diminished relative to the second SPB (Figure 7B). ------- COMMENT: 09ee8fea7eb8ca88 25 N7dwNEfLLPU6dKnnxxWxQt0zO+g At 25°C, Sid4-GFP SPB intensity was reduced by 4 approximately 30% in ppc89-3 and 60% in ppc89-4 compared to wildtype (Figures 2A 5 and S2A). At 36°C, Sid4-GFP SPB intensity was further reduced by ~90% in ppc89-3 6 and ppc89-4 compared to wildtype (Figure 2A,B). These results indicate that the Sid4- 7 Ppc89 interaction is disrupted in both ppc89-3 and ppc89-4 cells at restrictive 8 temperatu re. ------- COMMENT: 09ee8fea7eb8ca88 26 N7dwNEfLLPU6dKnnxxWxQt0zO+g At 25°C, Sid4-GFP SPB intensity was reduced by 4 approximately 30% in ppc89-3 and 60% in ppc89-4 compared to wildtype (Figures 2A 5 and S2A). At 36°C, Sid4-GFP SPB intensity was further reduced by ~90% in ppc89-3 6 and ppc89-4 compared to wildtype (Figure 2A,B). These results indicate that the Sid4- 7 Ppc89 interaction is disrupted in both ppc89-3 and ppc89-4 cells at restrictive 8 temperatu re. ------- COMMENT: 09ee8fea7eb8ca88 27 qq9/Lqeaw+1uCj3K4MmzPmoaPu8 Both Ppc89(1-707)-mNG and Sid4-RFP 8 localized to the SPB with comparable intensity to wildtype at both 25 ̊C and 36°C 9 (Figure 3D,E). ------- COMMENT: 09ee8fea7eb8ca88 28 qq9/Lqeaw+1uCj3K4MmzPmoaPu8 Both Ppc89(1-707)-mNG and Sid4-RFP 8 localized to the SPB with comparable intensity to wildtype at both 25 ̊C and 36°C 9 (Figure 3D,E). ------- COMMENT: 09ee8fea7eb8ca88 29 WQXRC/wtCH616htESJJX4yxQT5Q As expected, Dma1- 22 mNG, Cdc11-GFP and Mto1-mNG were lost from SPBs in ppc89-3 and ppc89-4 cells at 23 restrictive temperature, but Mto1-mNG localized normally to SPBs in ppc89(1-707) cells (Figures 4A-G; S2B and C) ------- COMMENT: 09ee8fea7eb8ca88 30 WQXRC/wtCH616htESJJX4yxQT5Q As expected, Dma1- 22 mNG, Cdc11-GFP and Mto1-mNG were lost from SPBs in ppc89-3 and ppc89-4 cells at 23 restrictive temperature, but Mto1-mNG localized normally to SPBs in ppc89(1-707) cells (Figures 4A-G; S2B and C) ------- COMMENT: 09ee8fea7eb8ca88 31 WQXRC/wtCH616htESJJX4yxQT5Q As expected, Dma1- 22 mNG, Cdc11-GFP and Mto1-mNG were lost from SPBs in ppc89-3 and ppc89-4 cells at 23 restrictive temperature, but Mto1-mNG localized normally to SPBs in ppc89(1-707) cells (Figures 4A-G; S2B and C) ------- COMMENT: 09ee8fea7eb8ca88 32 WQXRC/wtCH616htESJJX4yxQT5Q As expected, Dma1- 22 mNG, Cdc11-GFP and Mto1-mNG were lost from SPBs in ppc89-3 and ppc89-4 cells at 23 restrictive temperature, but Mto1-mNG localized normally to SPBs in ppc89(1-707) cells (Figures 4A-G; S2B and C) ------- COMMENT: 09ee8fea7eb8ca88 34 WQXRC/wtCH616htESJJX4yxQT5Q As expected, Dma1- 22 mNG, Cdc11-GFP and Mto1-mNG were lost from SPBs in ppc89-3 and ppc89-4 cells at 23 restrictive temperature, but Mto1-mNG localized normally to SPBs in ppc89(1-707) cells (Figures 4A-G; S2B and C) ------- COMMENT: 09ee8fea7eb8ca88 35 WQXRC/wtCH616htESJJX4yxQT5Q As expected, Dma1- 22 mNG, Cdc11-GFP and Mto1-mNG were lost from SPBs in ppc89-3 and ppc89-4 cells at 23 restrictive temperature, but Mto1-mNG localized normally to SPBs in ppc89(1-707) cells (Figures 4A-G; S2B and C) ------- COMMENT: 09ee8fea7eb8ca88 36 FM8bqSl/xFKnX/v9JRSt4qNxmi8 we also observed a reduction 3 in Mto2-mNG SPB signal at 36 ̊C in ppc89-3 and ppc89-4 compared to wildtype (Figure 4 S2D,E). ------- COMMENT: 09ee8fea7eb8ca88 37 FM8bqSl/xFKnX/v9JRSt4qNxmi8 we also observed a reduction 3 in Mto2-mNG SPB signal at 36 ̊C in ppc89-3 and ppc89-4 compared to wildtype (Figure 4 S2D,E). ------- COMMENT: 09ee8fea7eb8ca88 38 4+q/8yWHiy1d/wEdZ1qISEIb9i0 Neither Sfi1-mCherry nor 7 Sad1-mCherry signal were reduced in ppc89-3 or ppc89-4 cells (Figure S3A,B). ------- COMMENT: 09ee8fea7eb8ca88 39 4+q/8yWHiy1d/wEdZ1qISEIb9i0 Neither Sfi1-mCherry nor 7 Sad1-mCherry signal were reduced in ppc89-3 or ppc89-4 cells (Figure S3A,B). ------- COMMENT: 09ee8fea7eb8ca88 40 VLi2y7B8w6yV2i3B4i7XK4K11Qo Pcp1 showed ~50% 9 reduction in pcp89-4 cells (Figure S3C) ------- COMMENT: 09ee8fea7eb8ca88 41 uZVAm1h5m0WwVvPeH7NeC/Mq6WQ ... so we tested whether tethering Sid4 to 23 Pcp1-GFP would rescue ppc89-3 and if so, ppc89-4 as well. ppc89-3 was rescued when Sid4 was tethered to Pcp1-GFP, as it had been when tethered to Ppc89-3 directly 2 (Figure S3D) ------- COMMENT: 09ee8fea7eb8ca88 42 XLbI8jSmRwX87CDKCIry11U5z8s Can grow at 32°C but not at 36°C | Sid4 tethered to 4 the SPB via Pcp1-GFP did not rescue growth of ppc89-4 cells at 36°C but the ppc89-4 5 pcp1-GFP sid4-mCherry strain grew at 32°C, and imaging confirmed that Sid4-GBP- 6 mCherry but not Sid4-mCherry was present at the SPB at 36 ̊C (Figures 5B and S4A). ------- COMMENT: 09ee8fea7eb8ca88 43 u6EAhz6vmFtwEeXDad/geHLi1Rc The addition of the tagged sid4 and pcp1 alleles 13 exacerbated the cell growth (Figures 5B and S3D) and cell division defects of the ppc89 14 ts alleles (Figures 1C,D and 5C,D) In contrast, ppc89-4 cells with Sid4 tethered to the SPB displayed a significant 17 percentage of uninucleate cells with an off-center septum and also multiple Ppc89-4 foci 18 (Figures 5C,D and S4). ------- COMMENT: 09ee8fea7eb8ca88 45 e/gjcnO/XUMirzoorYBmqmDhiUs We also determined 16 that the rarer off-center septum phenotype (9 of 280 cells) arose because a second 17 cytokinetic ring formed at the new cell end of one daughter cell after its birth (Figure 18 7C). In these cells, the cytokinetic ring appeared not to fully disassemble and one 19 daughter inherited this cortical material. Then, a second cytokinetic ring formed at 20 variable times relative to completion of the previous division where the first cytokinetic 21 ring remnants were located (Figure 7C). ------- COMMENT: 09ee8fea7eb8ca88 48 PAlSCsNJoWMQnn/zm8kYnyn7T7s figure 7 ------- COMMENT: 09f311273c61ffe7 1 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: 0a18d3058ad0058a 1 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 0a18d3058ad0058a 2 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 0a18d3058ad0058a 3 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 0a18d3058ad0058a 4 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 0a18d3058ad0058a 5 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 0a18d3058ad0058a 6 uea/SMia5E64tkzXDlnxMYBY2nA (comment: data not shown) ------- COMMENT: 0a18d3058ad0058a 7 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 0a18d3058ad0058a 8 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 0a18d3058ad0058a 9 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: 0a18d3058ad0058a 10 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: 0a18d3058ad0058a 11 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 0a18d3058ad0058a 12 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 0a18d3058ad0058a 15 5a7fvmcTov3dOR2/Er4Wu3j4EM0 (Fig. 3A and B) ------- COMMENT: 0a18d3058ad0058a 16 5a7fvmcTov3dOR2/Er4Wu3j4EM0 (Fig. 3A and B) ------- COMMENT: 0a18d3058ad0058a 17 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 0a18d3058ad0058a 18 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 0a4aae8859bbb8db 1 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 0a4aae8859bbb8db 2 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 0a4aae8859bbb8db 3 AOYuh7dL/YHR7SDOO0sTQx7XCZ4 Figure S1A ------- COMMENT: 0a4aae8859bbb8db 4 AOYuh7dL/YHR7SDOO0sTQx7XCZ4 Figure S1A ------- COMMENT: 0a4aae8859bbb8db 5 yZGCad3gpKkUKIrYoIreyKpm6v0 results in a significant growth advantage of BFC mutant over wild-type strains when equal number of log phase cells are grown on rapamycin-containing EMM plates supplemented with amino acids (Figures S1A and S1B). Moreover, treating cells with 125 or 150 ng/mL of rapamycin for 90 min revealed that the growth advantage of BFC mutants correlates with an increase in P-Rps6 levels compared to wild-type cells (Figure S5C). Together, these data support a model in which BFC participates in TORC1 repression. ------- COMMENT: 0a4aae8859bbb8db 9 4NMkodkyxKC+RTtPyYLbKqc7CtU Bhd1 and Fnp1 appear diffusely distributed throughout the cytoplasm in amino acid replete conditions, but localize to vacuoles in response to amino acid starvation evidenced by the strong overlap between Bhd1-/Fnp1-GFP and the FM4-64 dye under amino acid starvation conditions (Figures 2A–2F) ------- COMMENT: 0a4aae8859bbb8db 13 fJM6FR5G6IUv3HhcAwCx1ZPMn2s (figure 4d) (comment: vw: assayed vacuolar pH as a surrogate for V-ATPase activity) ------- COMMENT: 0a4aae8859bbb8db 14 fJM6FR5G6IUv3HhcAwCx1ZPMn2s (figure 4d) (comment: vw: assayed vacuolar pH as a surrogate for V-ATPase activity) ------- COMMENT: 0a4aae8859bbb8db 15 k1JPtSnySNQsA6eFrWp7dpvCn7Q (Figure 4F) (comment: CONDITION ph9) ------- COMMENT: 0a4aae8859bbb8db 16 k1JPtSnySNQsA6eFrWp7dpvCn7Q (Figure 4F) (comment: CONDITION ph9) ------- COMMENT: 0a4aae8859bbb8db 21 01JwaKyZtGTyaAVfEo6BcJMakJU (comment: found in all 1- and 2-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 22 01JwaKyZtGTyaAVfEo6BcJMakJU (comment: found in all 1- and 2-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 24 01JwaKyZtGTyaAVfEo6BcJMakJU (comment: found in all 1- and 2-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 25 01JwaKyZtGTyaAVfEo6BcJMakJU (comment: found in all 1- and 2-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 26 01JwaKyZtGTyaAVfEo6BcJMakJU (comment: found in all 1- and 2-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 27 01JwaKyZtGTyaAVfEo6BcJMakJU (comment: found in all 1- and 2-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 28 01JwaKyZtGTyaAVfEo6BcJMakJU (comment: found in all 1- and 2-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 29 YSjmVhST1StGSVEvMWRbNK6tC2k ((comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 30 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 31 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 32 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 33 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 34 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 35 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 36 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 37 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 38 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 39 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 40 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 41 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 42 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 43 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 44 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 45 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 46 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 47 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 48 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 49 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 50 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 51 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 52 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 53 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 54 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 55 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 56 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 57 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 58 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 59 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 60 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 61 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 62 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 63 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 64 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 65 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 66 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 67 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 68 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 69 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 70 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 71 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 72 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 73 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 74 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 75 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 76 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 77 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 78 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 79 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 80 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 81 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 82 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 83 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 84 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 85 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 86 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 87 GRutyginn1KIQhzrGn23G2weBj4 (comment: Common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 88 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 89 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 90 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 91 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 92 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 93 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 94 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 95 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 96 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 97 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 98 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 99 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 100 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 101 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 102 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 103 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 104 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 105 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 106 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 107 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 108 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 109 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 110 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 111 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 112 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 113 YSjmVhST1StGSVEvMWRbNK6tC2k (comment: common to one- and two-step TAP purifications) ------- COMMENT: 0a4aae8859bbb8db 116 d28zaDp/UehEHPFuhSoMbLlUTP4 (Figure S1A) ------- COMMENT: 0a4aae8859bbb8db 117 d28zaDp/UehEHPFuhSoMbLlUTP4 (Figure S1A) ------- COMMENT: 0a4aae8859bbb8db 120 zFUgVQcs9Gp5H0ZVc1AHCcH8sSI (Figure S3) Bhd1 and Fnp1 localize to vacuoles in response to amino acid star- vation and that this localization is largely independent of the presence of the other protein. ------- COMMENT: 0a4aae8859bbb8db 121 zFUgVQcs9Gp5H0ZVc1AHCcH8sSI (Figure S3) Bhd1 and Fnp1 localize to vacuoles in response to amino acid star- vation and that this localization is largely independent of the presence of the other protein. ------- COMMENT: 0a4aae8859bbb8db 122 Cq81U9Hizx6+PlZBkkpLC62aNh0 (comment: CONDITION during amino acid supplementation) (Figures 2G and S4), demonstrating that the BFC, is required for efficient activation of TORC1 following amino acid supplementation. ------- COMMENT: 0a4aae8859bbb8db 123 Cq81U9Hizx6+PlZBkkpLC62aNh0 (comment: CONDITION during amino acid supplementation) (Figures 2G and S4), demonstrating that the BFC, is required for efficient activation of TORC1 following amino acid supplementation. ------- COMMENT: 0a4aae8859bbb8db 124 O+YchmjmCZvKx7r5DXVk1wW50jg BFC augments TORC1 activation in response to amino acid stimulation ------- COMMENT: 0a4aae8859bbb8db 128 f7XkFOvs9XpdANV/ygdS/UR1w8w (comment: CONDITION during amino acid starvation) ------- COMMENT: 0a4aae8859bbb8db 129 f7XkFOvs9XpdANV/ygdS/UR1w8w (comment: CONDITION during amino acid starvation) ------- COMMENT: 0a79c8dca3733456 1 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 0a79c8dca3733456 2 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 0a79c8dca3733456 3 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 0a79c8dca3733456 4 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 0a79c8dca3733456 5 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 0a79c8dca3733456 6 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 0a79c8dca3733456 7 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 0a79c8dca3733456 8 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 0a79c8dca3733456 9 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 0a79c8dca3733456 10 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 0a79c8dca3733456 11 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 0a79c8dca3733456 13 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 0a79c8dca3733456 14 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 0a79c8dca3733456 15 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 0a79c8dca3733456 16 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 0a79c8dca3733456 17 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 0a79c8dca3733456 18 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 0a79c8dca3733456 19 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 0a79c8dca3733456 20 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 0a79c8dca3733456 21 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 0a79c8dca3733456 22 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 0a79c8dca3733456 23 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 0a79c8dca3733456 24 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 0a79c8dca3733456 25 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 0a79c8dca3733456 26 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 0a79c8dca3733456 27 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 0a79c8dca3733456 28 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 0a79c8dca3733456 29 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 0ae5e45bd4264f63 1 nUCcEDoowWdBc8w5z3A4wbzAAHc Figure 1F ------- COMMENT: 0ae5e45bd4264f63 2 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 0ae5e45bd4264f63 3 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 0ae5e45bd4264f63 4 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 0ae5e45bd4264f63 5 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 0ae5e45bd4264f63 6 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 0ae5e45bd4264f63 7 VaXiSZZO7R52KMDhEepRtSmtYgA Fig.1 ------- COMMENT: 0ae5e45bd4264f63 8 VaXiSZZO7R52KMDhEepRtSmtYgA Fig.1 ------- COMMENT: 0ae5e45bd4264f63 9 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 0ae5e45bd4264f63 10 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 0ae5e45bd4264f63 11 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 0ae5e45bd4264f63 12 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 0ae5e45bd4264f63 13 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 0ae5e45bd4264f63 14 iAcbpl1ew+nFwb8AiAqOLZxpvN8 Figure 1D ------- COMMENT: 0ae5e45bd4264f63 15 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 0ae5e45bd4264f63 16 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 0ae5e45bd4264f63 17 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 0ae5e45bd4264f63 18 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 0ae5e45bd4264f63 20 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 0ae5e45bd4264f63 21 gC+OPtCC43GyDCtaG9nh6Kn4Rmc Figure S1C ------- COMMENT: 0ae5e45bd4264f63 22 gC+OPtCC43GyDCtaG9nh6Kn4Rmc Figure S1C ------- COMMENT: 0ae5e45bd4264f63 23 AOYuh7dL/YHR7SDOO0sTQx7XCZ4 Figure S1A ------- COMMENT: 0ae5e45bd4264f63 24 7Qgl6Vc4Wg8+hYCM9rRxuCeWUnI Figure S1B ------- COMMENT: 0ae5e45bd4264f63 25 fRGpCLYUKZw0hQxyGfyxCTNQqGI Fig. 1, Fig. 1B, Fig. 2D ------- COMMENT: 0ae5e45bd4264f63 27 PvjQtxli7DciL0hRjGNDFvMsg78 Figure 1C &F ------- COMMENT: 0ae5e45bd4264f63 28 LQmuKZbL9ND56pzzWctDT58tWRE Figure 3C ------- COMMENT: 0ae5e45bd4264f63 29 qRuiFIUfCEaRK2U9+oSKa18znxI Figure 3E ------- COMMENT: 0ae5e45bd4264f63 30 qRuiFIUfCEaRK2U9+oSKa18znxI Figure 3E ------- COMMENT: 0ae5e45bd4264f63 31 LQmuKZbL9ND56pzzWctDT58tWRE Figure 3C ------- COMMENT: 0ae5e45bd4264f63 32 kaUoUdRn/Ot3vRCEG3xletcSM28 Figure 3B Furthermore, in cells overproducing ank1+, ~10% of Fim1-mCherry patches internalized compared to ~95% of Fim1-mCherry patches internalized in the control cells (Fig. 3B) ------- COMMENT: 0ae5e45bd4264f63 33 zMZv8FTks8B7h7e3RnE12l2jvHU Figure 3A in myo1-mNG fim1-mCherry cells overproducing Ank1, while Fim1-mCherry was at patches, almost all Myo1-mNG was cytoplasmic (Fig. 3A). ------- COMMENT: 0ae5e45bd4264f63 34 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 0ae5e45bd4264f63 35 aOqYL7F2KV5d9E4mVC2uoiGmluk Figure 1E ------- COMMENT: 0ae5e45bd4264f63 36 CPtfNCCJL1ySb3M6MxHXDn+E2Wc Figure S1D ------- COMMENT: 0ae5e45bd4264f63 37 CPtfNCCJL1ySb3M6MxHXDn+E2Wc Figure S1D ------- COMMENT: 0ae5e45bd4264f63 38 RbT95OLcWEedu8kbG/3/+11Yn+8 Figure S1D (comment: vw: same pathway) ------- COMMENT: 0ae5e45bd4264f63 41 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 0ae5e45bd4264f63 42 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 0ae5e45bd4264f63 44 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 0ae5e45bd4264f63 46 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 0ae5e45bd4264f63 47 g3Lp92pznz+yZL4Fc/M0Eq7MTns Given the AF model and the above results, we hypothesized that Ank1 In contrast, in myo1-mNG fim1-mCherry cells overproducing Ank1, while Fim1-mCherry was at patches, almost all Myo1-mNG was cytoplasmic (Fig. 3A). These data support our model that Ank1 blocks Myo1 membrane association and normally sequesters the bulk of Myo1 in the cytoplasm.precludes Myo1 membrane binding and sequesters the myosin-1 complex in the cytoplasm (Fig. 2F).Addition of Ank1 resulted in a reduction in the amount of co-pelleting of the Myo1(1-964)-FLAG-Cam1-Cam2 complex with liposomes (Fig. 2F). We therefore conclude that Ank1 directly binds and inhibits Myo1 membrane binding, supporting our model. ------- COMMENT: 0ae5e45bd4264f63 48 xqiexmfmgmxd1V1aO2Xh9bw1NHY (comment: vw: I think we can conclude this from the EXP, especially as we know that myo1 binds phopshilipids in other species) Addition of Ank1 resulted in a reduction in the amount of co-pelleting of the Myo1(1-964)-FLAG-Cam1-Cam2 complex with liposomes (Fig. 2F). We therefore conclude that Ank1 directly binds and inhibits Myo1 membrane binding, supporting our model. ------- COMMENT: 0afd82c6e0085a3f 12 psAvewk5kqTLdkmd7aBnEdaLIY8 ------- COMMENT: 0afd82c6e0085a3f 13 reS6YxLFiGRbJUdLrJnfTFxlhDE ------- COMMENT: 0b0990bc7e9460b7 48 YMpLYFN/zQocLcpJnVnDwg+1Msc (comment: CONDITION observed after short-duration overexpression) ------- COMMENT: 0b0990bc7e9460b7 64 rof8MLm+gOOhxTK9gBdimxOA5lI (comment: CONDITION temperature restrictive for cdc25-22) ------- COMMENT: 0b0990bc7e9460b7 65 rof8MLm+gOOhxTK9gBdimxOA5lI (comment: CONDITION temperature restrictive for cdc25-22) ------- COMMENT: 0b0990bc7e9460b7 66 rof8MLm+gOOhxTK9gBdimxOA5lI (comment: CONDITION temperature restrictive for cdc25-22) ------- COMMENT: 0b0990bc7e9460b7 67 WtMaRpR1wnU5KiqbTeg5hC7rzGw assayed using N-terminal Rng2-Ns fragment or calponin homology domain (CHD) fragment ------- COMMENT: 0b586801d9eddd09 1 pa5HegjfRUUQOOZWMDsqjrn0iHk The single and merged images indicate that CAA20785 colocalizes with Sid4p to SPBs throughout the cell cycle (Figure 2B). ------- COMMENT: 0b586801d9eddd09 2 pa5HegjfRUUQOOZWMDsqjrn0iHk The single and merged images indicate that CAA20785 colocalizes with Sid4p to SPBs throughout the cell cycle (Figure 2B). ------- COMMENT: 0b586801d9eddd09 3 z+HWcaT1jRGN7E6LsRH8WsGKuew The CAA20785 GFP fusion protein localized normally in cdc16-116, spg1-106, cdc7-24, and sid2-250 temperature-sensitive mutants...(figure 3) ------- COMMENT: 0b586801d9eddd09 4 z+HWcaT1jRGN7E6LsRH8WsGKuew The CAA20785 GFP fusion protein localized normally in cdc16-116, spg1-106, cdc7-24, and sid2-250 temperature-sensitive mutants...(figure 3) ------- COMMENT: 0b586801d9eddd09 5 z+HWcaT1jRGN7E6LsRH8WsGKuew The CAA20785 GFP fusion protein localized normally in cdc16-116, spg1-106, cdc7-24, and sid2-250 temperature-sensitive mutants...(figure 3) ------- COMMENT: 0b586801d9eddd09 6 z+HWcaT1jRGN7E6LsRH8WsGKuew The CAA20785 GFP fusion protein localized normally in cdc16-116, spg1-106, cdc7-24, and sid2-250 temperature-sensitive mutants...(figure 3) ------- COMMENT: 0b586801d9eddd09 7 J1Ix6m750MI/aClOiWf062oGUDY How- ever, its localization to the SPB was lost in the sid4-SA1 mutant at restrictive temperature (Figure 3). ------- COMMENT: 0b586801d9eddd09 8 Nt5YFLuaNGlwqfwR9pmUQjWO0W4 At 36°C, Spg1p-GFP was detected at SPBs in wild-type cells but was absent from SPBs in the cdc11 mutant strains (Figure 4B). ------- COMMENT: 0b586801d9eddd09 9 Nt5YFLuaNGlwqfwR9pmUQjWO0W4 At 36°C, Spg1p-GFP was detected at SPBs in wild-type cells but was absent from SPBs in the cdc11 mutant strains (Figure 4B). ------- COMMENT: 0b586801d9eddd09 10 JX6TSuokKYdFOcJZXUUiCED3klI GFP- Cdc11p(1– 630) was distributed throughout the cytoplasm (our unpublished results), but GFP-Cdc11p(631–1045) local- ized to SPBs (Figure 5A). ------- COMMENT: 0b586801d9eddd09 11 JX6TSuokKYdFOcJZXUUiCED3klI GFP- Cdc11p(1– 630) was distributed throughout the cytoplasm (our unpublished results), but GFP-Cdc11p(631–1045) local- ized to SPBs (Figure 5A). ------- COMMENT: 0b586801d9eddd09 12 4ydwj7iCga/hwTL2OzLPKLveWT8 Interestingly, we found that overproduction of GFP-Cdc11p(631–1045) gener-ated a sid phenotype (Figure 5A). ------- COMMENT: 0b586801d9eddd09 13 Xw2k4Fs/kdYzbVAxhdwRA/m/Su4 Overproduction of Cdc11p(631–1045) had no effect on the localization of Sid4p-GFP but caused the loss of Cdc11p-GFP and Spg1p-GFP from SPBs (Figure 5B). This is consistent with the with the idea that Cdc11p(631–1045) saturates the SPB bind- ing site for Cdc11p, thus eliminating the opportunity for the full-length protein to localize to the SPB. ------- COMMENT: 0b586801d9eddd09 14 bLWEQbkofWt2pu2R65D6qeKHVC8 Overproduction of Cdc11p(631–1045) had no effect on the localization of Sid4p-GFP but caused the loss of Cdc11p-GFP and Spg1p-GFP from SPBs (Figure 5B). ------- COMMENT: 0bca2cbb0e3b6375 2 iV0NogpEU/XZg30M7yTokfHnQzc Consistent with the phe- notypes of deficiency of SKY1 and SRPKs, dsk1Δ also exhibited cisPt resistance.........(Figure 1a and Figure S1c). ------- COMMENT: 0bca2cbb0e3b6375 3 uHA5mnmeNq+xXWdcMY9hfsCZTA8 In addition, dsk1Δ was consistently but mar- ginally sensitive to HU. .........(Figure 1a and Figure S1c). ------- COMMENT: 0bca2cbb0e3b6375 4 kFd5xGn3TPnJGzjm+eSWCoW9nvQ Interestingly, we found that dsk1Δ was highly sensitive to replication-associated DNA break agents like camptothecin (CPT), which is a topoisomerase I inhibitor, and was slightly sensitive to methyl methanesulfonate (MMS), which alkylates and breaks DNA during replication (Figure 1a and Figure S1c). ------- COMMENT: 0bca2cbb0e3b6375 5 kFd5xGn3TPnJGzjm+eSWCoW9nvQ Interestingly, we found that dsk1Δ was highly sensitive to replication-associated DNA break agents like camptothecin (CPT), which is a topoisomerase I inhibitor, and was slightly sensitive to methyl methanesulfonate (MMS), which alkylates and breaks DNA during replication (Figure 1a and Figure S1c). ------- COMMENT: 0bca2cbb0e3b6375 6 RzWbrq61EGmBt0gc9EomPOlUOAI However, dsk1Δ was not sensitive to Bleomycin (Bleo), Phleomycin (Phleo), and UV irradiation, which directly break DNA, as well as 6-Azauracil (6-AU) and Mycophenolic acid (MPA), which impair transcription (Figure S1d). ------- COMMENT: 0bca2cbb0e3b6375 7 RzWbrq61EGmBt0gc9EomPOlUOAI However, dsk1Δ was not sensitive to Bleomycin (Bleo), Phleomycin (Phleo), and UV irradiation, which directly break DNA, as well as 6-Azauracil (6-AU) and Mycophenolic acid (MPA), which impair transcription (Figure S1d). ------- COMMENT: 0bca2cbb0e3b6375 8 RzWbrq61EGmBt0gc9EomPOlUOAI However, dsk1Δ was not sensitive to Bleomycin (Bleo), Phleomycin (Phleo), and UV irradiation, which directly break DNA, as well as 6-Azauracil (6-AU) and Mycophenolic acid (MPA), which impair transcription (Figure S1d). ------- COMMENT: 0bca2cbb0e3b6375 9 RzWbrq61EGmBt0gc9EomPOlUOAI However, dsk1Δ was not sensitive to Bleomycin (Bleo), Phleomycin (Phleo), and UV irradiation, which directly break DNA, as well as 6-Azauracil (6-AU) and Mycophenolic acid (MPA), which impair transcription (Figure S1d). ------- COMMENT: 0bca2cbb0e3b6375 10 RzWbrq61EGmBt0gc9EomPOlUOAI However, dsk1Δ was not sensitive to Bleomycin (Bleo), Phleomycin (Phleo), and UV irradiation, which directly break DNA, as well as 6-Azauracil (6-AU) and Mycophenolic acid (MPA), which impair transcription (Figure S1d). ------- COMMENT: 0bca2cbb0e3b6375 11 swwcEUYY/kvy5CnkUXOCNiVmzLw It showed CPT sensitivity compared with its isogenic dsk1-5FLAG control, but not as severe as dsk1Δ (Figure 1d). To avoid the negative effects of the FLAG tag, we cre- ated a dsk1-K110A mutant without the tag, and it also showed the sensitivity of CPT (Figure S1e) ------- COMMENT: 0bca2cbb0e3b6375 12 92lQ0zOOrF2OzgaJlEBVfcbwNSI In line with this, the spacer truncation mutant was also sensi- tive to CPT (Figure 1e). ------- COMMENT: 0bca2cbb0e3b6375 13 ZWDuG4+hVehRQYhyeubEOnhT9e0 We then overexpressed dsk1+ from the constitutive adh21 pro- moter with medium strength at the lys1 locus of S. pombe ge- nome (Chen et al. 2017). This made the cells highly sensitive to CPT and marginally sensitive to MMS (Figure 1g). ------- COMMENT: 0bca2cbb0e3b6375 14 ZWDuG4+hVehRQYhyeubEOnhT9e0 We then overexpressed dsk1+ from the constitutive adh21 pro- moter with medium strength at the lys1 locus of S. pombe ge- nome (Chen et al. 2017). This made the cells highly sensitive to CPT and marginally sensitive to MMS (Figure 1g). ------- COMMENT: 0bca2cbb0e3b6375 16 kFd5xGn3TPnJGzjm+eSWCoW9nvQ Interestingly, we found that dsk1Δ was highly sensitive to replication-associated DNA break agents like camptothecin (CPT), which is a topoisomerase I inhibitor, and was slightly sensitive to methyl methanesulfonate (MMS), which alkylates and breaks DNA during replication (Figure 1a and Figure S1c). ------- COMMENT: 0bca2cbb0e3b6375 17 kFd5xGn3TPnJGzjm+eSWCoW9nvQ Interestingly, we found that dsk1Δ was highly sensitive to replication-associated DNA break agents like camptothecin (CPT), which is a topoisomerase I inhibitor, and was slightly sensitive to methyl methanesulfonate (MMS), which alkylates and breaks DNA during replication (Figure 1a and Figure S1c). ------- COMMENT: 0bca2cbb0e3b6375 18 yLuOOgoZHYc9dRbH2bHUEmXvWDc However, dsk1Δ combined with muta- tions of DNA damage checkpoint effector chk1Δ and replication checkpoint effector cds1Δ, exhibited synergistic CPT and HU sensitivities (Figure S2b,c). ------- COMMENT: 0bca2cbb0e3b6375 19 yLuOOgoZHYc9dRbH2bHUEmXvWDc However, dsk1Δ combined with muta- tions of DNA damage checkpoint effector chk1Δ and replication checkpoint effector cds1Δ, exhibited synergistic CPT and HU sensitivities (Figure S2b,c). ------- COMMENT: 0bca2cbb0e3b6375 20 uwFkV91cEEsth87eBXVnTAZqFig Additionally, there were few septa when both dsk1+ and dsk1Δ were treated with MMS, and their number of septa was similarly increased after MMS release (Figure S2d). This result indicates that the cell cycle progres- sion of dsk1Δ under MMS is arrested by functional checkpoints. To further support it biochemically, we tagged Chk1 with 3HA as we had no antibody against S. pombe Chk1. We found that the 3HA tag did not influence the genotoxic phenotype of chk1+ (Figure S2e). Immunoblots showed that Chk1-3HA in dsk1Δ was normally phosphorylated after MMS treatment (Figure S2f). Therefore, we conclude that dsk1Δ does not influence check- points activation. ------- COMMENT: 0bca2cbb0e3b6375 21 WaAdvzpNkOgTYjVamwy+ccR197w Moreover, we found that dsk1Δ together with mutations of PRR genes rhp18Δ (translesion synthesis) (Figure S2g) and ubc13Δ (template switching) (Figure S2h) were additively defective in response to CPT and MMS treatments, suggesting that Dsk1 and PRR are in parallel and distinct pathways in response to replica- tion stress. ------- COMMENT: 0bca2cbb0e3b6375 22 WaAdvzpNkOgTYjVamwy+ccR197w Moreover, we found that dsk1Δ together with mutations of PRR genes rhp18Δ (translesion synthesis) (Figure S2g) and ubc13Δ (template switching) (Figure S2h) were additively defective in response to CPT and MMS treatments, suggesting that Dsk1 and PRR are in parallel and distinct pathways in response to replica- tion stress. ------- COMMENT: 0bca2cbb0e3b6375 23 UxNveR7w999mKZrF9lXlWrs8EAc We exploited the YFP-tagged Rad52 strain and found that the percentage (45%) of Rad52-YFP fluorescent foci in dsk1Δ cells was elevated almost 100-fold relative to the percentage (0.5%) of that in WT cells under physiological conditions, suggesting more spontaneous DNA damage induced by dsk1Δ. ------- COMMENT: 0bca2cbb0e3b6375 24 YYypd8huP1QDL6Yquekws/wmoGw After CPT release, the percentage of Rad52 foci in dsk1Δ decreased more slowly than that in dsk1+ (Figures 2a and S3a,b), indicating a compromised HR repair in dsk1Δ. Second, we exploited a transformation-based genetic system (Figure 2b) ------- COMMENT: 0bca2cbb0e3b6375 25 Id3ZjNg30N6cpKOwW8j9UDxwBvk As expected, the HR frequency of leu1 integration in rad51Δ cells was too low to be displayed. We also found that the HR frequency of leu1 integration in dsk1Δ cells was significantly reduced (Figure 2c), suggesting that Dsk1 may regulate the Rad52- and Rad51-dependent gene conversion sub-pathway of HR. ------- COMMENT: 0bca2cbb0e3b6375 26 vfiRS+oB2EtFXGGvKLTahCGi/Us On the contrary, dsk1Δ cells displayed about a fivefold reduction in spontaneous recombination rate, mainly in the aspect of gene conversion rather than gene deletion (Figure 2g). ------- COMMENT: 0bca2cbb0e3b6375 27 zVvvg0sGsAXSHcgv13+9HZlfTnU Consistent with the previous study (Hayashi et al. 2009), Dsk1-GFP local- ized in both the nucleus and cytoplasm. After CPT treatment, the percentage of Dsk1-GFP translocated from cytoplasm to nucleus was increased, and dominantly accumulated in the nu- cleus (Figures 3a and S3c). ------- COMMENT: 0bca2cbb0e3b6375 28 zVvvg0sGsAXSHcgv13+9HZlfTnU Consistent with the previous study (Hayashi et al. 2009), Dsk1-GFP local- ized in both the nucleus and cytoplasm. After CPT treatment, the percentage of Dsk1-GFP translocated from cytoplasm to nucleus was increased, and dominantly accumulated in the nu- cleus (Figures 3a and S3c). ------- COMMENT: 0bca2cbb0e3b6375 29 yXfzTmFr6wP5bM2yvSEJL/+PMSo Figure 4 (comment: Dsk1 phosphorylates Rad52 in vitro and in vivo). ------- COMMENT: 0bca2cbb0e3b6375 30 jGxVc8LwR9d+Rg4E5lNZ0RUqM3k We also purified 6His-Rad52-S365A and 6His-Rad52-S367A proteins and found that the abundance of the phosphorylated peptide of Rad52-S367A was significantly higher than that of Rad52-S365A when incubated with GST- Dsk1 (Figure 4f,g). Together, these in vitro data strongly indi- cate that Dsk1 directly phosphorylates Rad52-Ser365. Together, these results suggest that Dsk1-mediated Rad52-Ser365 phosphorylation is probably one of the mechanisms for Dsk1 regulating HR repair. ------- COMMENT: 0bca2cbb0e3b6375 31 oDwnMMyYoE8eBuOgXKBHxLwLyL8 We found that the lev- els of the indicated phosphorylated peptide of Rad52-YFP were substantially reduced in dsk1Δ, whereas no differences in the levels of unmodified peptides (Figure 4h). The remaining Rad52 phosphorylation in dsk1Δ suggests that there are redundant ki- nases of Rad52 in vivo. Moreover, Ser319 was predicted as the major phosphorylated residue in this indicated Rad52 peptide (Figure 4h). ------- COMMENT: 0bca2cbb0e3b6375 32 DtJzD602AhEe9yDyL2bTj/Exv2M The results of spot as- says revealed that rad52Δ strain as a positive control grew slowly and exhibited pronounced sensitivities to all tested drugs. FIGURE 5 | The defective genotoxic and HR phenotypes of Rad52-Ser365 phosphorylation-defective and -mimicking mutants ------- COMMENT: 0bca2cbb0e3b6375 33 DtJzD602AhEe9yDyL2bTj/Exv2M The results of spot as- says revealed that rad52Δ strain as a positive control grew slowly and exhibited pronounced sensitivities to all tested drugs. FIGURE 5 | The defective genotoxic and HR phenotypes of Rad52-Ser365 phosphorylation-defective and -mimicking mutants ------- COMMENT: 0bca2cbb0e3b6375 34 DtJzD602AhEe9yDyL2bTj/Exv2M The results of spot as- says revealed that rad52Δ strain as a positive control grew slowly and exhibited pronounced sensitivities to all tested drugs. FIGURE 5 | The defective genotoxic and HR phenotypes of Rad52-Ser365 phosphorylation-defective and -mimicking mutants ------- COMMENT: 0bca2cbb0e3b6375 35 DtJzD602AhEe9yDyL2bTj/Exv2M The results of spot as- says revealed that rad52Δ strain as a positive control grew slowly and exhibited pronounced sensitivities to all tested drugs. FIGURE 5 | The defective genotoxic and HR phenotypes of Rad52-Ser365 phosphorylation-defective and -mimicking mutants ------- COMMENT: 0bca2cbb0e3b6375 36 Z7f2P4BRDaVXKojewoJhNikbn0M The rad52-S365A-YFP was slightly sensitive to HU and CPT, but not to MMS and cisPt. ------- COMMENT: 0bca2cbb0e3b6375 37 Z7f2P4BRDaVXKojewoJhNikbn0M The rad52-S365A-YFP was slightly sensitive to HU and CPT, but not to MMS and cisPt. ------- COMMENT: 0bca2cbb0e3b6375 38 Z7f2P4BRDaVXKojewoJhNikbn0M The rad52-S365A-YFP was slightly sensitive to HU and CPT, but not to MMS and cisPt. ------- COMMENT: 0bca2cbb0e3b6375 39 Z7f2P4BRDaVXKojewoJhNikbn0M The rad52-S365A-YFP was slightly sensitive to HU and CPT, but not to MMS and cisPt. ------- COMMENT: 0bca2cbb0e3b6375 40 6yaWShVOwc+lTuL5vCEo8GJGslQ Moreover, the rad52-S365D/E-YFP displayed higher sensitivities to HU, CPT, and MMS rather than cisPt (Figure 5a). ------- COMMENT: 0bca2cbb0e3b6375 41 6yaWShVOwc+lTuL5vCEo8GJGslQ Moreover, the rad52-S365D/E-YFP displayed higher sensitivities to HU, CPT, and MMS rather than cisPt (Figure 5a). ------- COMMENT: 0bca2cbb0e3b6375 42 6yaWShVOwc+lTuL5vCEo8GJGslQ Moreover, the rad52-S365D/E-YFP displayed higher sensitivities to HU, CPT, and MMS rather than cisPt (Figure 5a). ------- COMMENT: 0bca2cbb0e3b6375 43 6yaWShVOwc+lTuL5vCEo8GJGslQ Moreover, the rad52-S365D/E-YFP displayed higher sensitivities to HU, CPT, and MMS rather than cisPt (Figure 5a). ------- COMMENT: 0bca2cbb0e3b6375 44 6yaWShVOwc+lTuL5vCEo8GJGslQ Moreover, the rad52-S365D/E-YFP displayed higher sensitivities to HU, CPT, and MMS rather than cisPt (Figure 5a). ------- COMMENT: 0bca2cbb0e3b6375 45 6yaWShVOwc+lTuL5vCEo8GJGslQ Moreover, the rad52-S365D/E-YFP displayed higher sensitivities to HU, CPT, and MMS rather than cisPt (Figure 5a). ------- COMMENT: 0bca2cbb0e3b6375 46 pDoUEjZZjqC5Ier/I3XYu6gEYuo When combined with dsk1Δ, obviously synthetic effects were observed for CPT treatment, and slightly additive effects were observed for HU and MMS treatment (Figure 5a), ------- COMMENT: 0bca2cbb0e3b6375 47 pDoUEjZZjqC5Ier/I3XYu6gEYuo When combined with dsk1Δ, obviously synthetic effects were observed for CPT treatment, and slightly additive effects were observed for HU and MMS treatment (Figure 5a), ------- COMMENT: 0bca2cbb0e3b6375 48 pDoUEjZZjqC5Ier/I3XYu6gEYuo When combined with dsk1Δ, obviously synthetic effects were observed for CPT treatment, and slightly additive effects were observed for HU and MMS treatment (Figure 5a), ------- COMMENT: 0bca2cbb0e3b6375 49 V5q+4qqurjpQYleaZk7YGQ5b4xo Furthermore, a high percentage of spontaneous Rad52-YFP foci was observed in rad52-S365D-YFP, but not in rad52-YFP and rad52-S365A-YFP, suggesting genomic instability of rad52- S365D-YFP. ------- COMMENT: 0bca2cbb0e3b6375 50 LyBbZfbpDDPiW/8SKVlCehqGl40 We found that the association affinity between Rad52-YFP and Rad51 was marginally affected by dsk1Δ (Figure S7b). ------- COMMENT: 0bca2cbb0e3b6375 51 jfvJQvktfG4qNgg76Wc2SOtSvGA There is an interaction between Dsk1 and Rad52, which can be verified by co-immunoprecipitation ------- COMMENT: 0bcdd20806ae5fa2 7 max4HEZNUxQSXwmzYVBOGtNvtHw (comment: CONDITION 25 degrees) ------- COMMENT: 0bcdd20806ae5fa2 8 max4HEZNUxQSXwmzYVBOGtNvtHw (comment: CONDITION 25 degrees) ------- COMMENT: 0bcdd20806ae5fa2 9 max4HEZNUxQSXwmzYVBOGtNvtHw (comment: CONDITION 25 degrees) ------- COMMENT: 0bcdd20806ae5fa2 10 WQjiN0o5LsKoVwP8h9f97Z55cko (comment: CONDITION 32 degrees) ------- COMMENT: 0bcdd20806ae5fa2 11 WQjiN0o5LsKoVwP8h9f97Z55cko (comment: CONDITION 32 degrees) ------- COMMENT: 0bcdd20806ae5fa2 12 max4HEZNUxQSXwmzYVBOGtNvtHw (comment: CONDITION 25 degrees) ------- COMMENT: 0bcdd20806ae5fa2 13 WQjiN0o5LsKoVwP8h9f97Z55cko (comment: CONDITION 32 degrees) ------- COMMENT: 0bcdd20806ae5fa2 16 R8SpP0a46JRfIbNx+ldmXLOKh44 (comment: CONDITION 25 or 32 degrees; latter semi-permissive for cdc8-27 alone) ------- COMMENT: 0bcdd20806ae5fa2 17 trDy7KYz3SVlNF1XoWefUXpQ/60 (comment: CONDITION 32 degrees; semi-permissive for cdc8-27 alone) ------- COMMENT: 0bf69081e6ca5d78 4 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: 0bf69081e6ca5d78 5 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: 0bf69081e6ca5d78 11 2f7WznNUitH10o0OzgqghXQ5TaI The data are consistent with a model in which its3p, like its mammalian homologue, can convert PI(3)P to PI(3,4)P2 and PI(3,4,5)P3. ------- COMMENT: 0bf69081e6ca5d78 13 w247zUzXXwJH4OFRHgCjSLZwJ3U (comment: vw fixed from GO:0052812 to GO:0016308 based on e-mail from Pgaudet) The data are consistent with a model in which its3p, like its mammalian homologue, can convert PI(3)P to PI(3,4)P2 and PI(3,4,5)P3. ------- COMMENT: 0bf69081e6ca5d78 14 sFWC+HWyCWz2e6/5HezG62WyU2s (comment: mishapen) ------- COMMENT: 0bf9eb6de2606a39 5 W4vhkTgjubmOw/zFOC7htyBGZ4A (comment: mitotic, in meiosis it is only n the kinetochore during meitoic division(metaphase/anaphase) not during prophase) ------- COMMENT: 0c03bd2b6873d1cc 1 KSIaU5tDiifyRx32IRs34VtOWyg Fig 1A appeared at the SPB upon conjugation of haploid cells, persisted until the onset of meiosis I, and disappeared thereafter ------- COMMENT: 0c03bd2b6873d1cc 6 1R+lmvZmtNxu3IIrKbNwVbi4hxs S1D ------- COMMENT: 0c03bd2b6873d1cc 7 SeegQD1aOYLy0oiYQpgqvNAvpC4 Interestingly, however, azygotic asci arising from diploid hrs1D cells did not show an apparent defect in spore formation (Figure S1D ------- COMMENT: 0c03bd2b6873d1cc 9 GDixkOuR6c+ZV/qtCxECytNWp0Y (comment: various exp, and ectoptic mitotic expression) ------- COMMENT: 0c03bd2b6873d1cc 10 GDixkOuR6c+ZV/qtCxECytNWp0Y (comment: various exp, and ectoptic mitotic expression) ------- COMMENT: 0c47374a70735395 1 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 0c47374a70735395 2 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 0c47374a70735395 3 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 0c47374a70735395 4 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 0c47374a70735395 5 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 0c47374a70735395 6 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 0c872033a21c238e 22 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: 0c872033a21c238e 23 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: 0cad091b0886ebbf 22 TORZ7aUtEgVVRNNmNzVVGDG3rQk ------- COMMENT: 0cc7abf864eaa55c 4 pqnTEmbiRPwQcKah135sHkw4ZII (comment: barely above background for vector alone and Sid1C) (Fig. 2B) ------- COMMENT: 0cc7abf864eaa55c 5 LfoJl6Y4BZ6B8RmIAXf9QIl9S64 (comment: a significant reduction in kinase activity, 40% of Sid1) ------- COMMENT: 0cd9347bf6391fc3 1 tHF7BL+GBE6Id+kr+H7w9IEUBqg (comment: cig2-cdc2) ------- COMMENT: 0cfe17c3c16e2b0c 1 NMycf5XJ9+YmhtjzakXf1yyYRF4 The Bub1 TPR domain is sufficient to form a complex with Mad3 ------- COMMENT: 0cfe17c3c16e2b0c 2 QOEClu0Ik8MYtIseAIEwVt8dSSE Bub1 co-immunoprecipitates with Mad3 in a manner that is dependent on their TPR domains, and independent of Bub3 ------- COMMENT: 0cfe17c3c16e2b0c 3 JjMrCxjd0TsuSBt3z5heCucRIz4 Figure 2B (comment: demonstrates robust arrest 3b 80% 12 hours) ------- COMMENT: 0cfe17c3c16e2b0c 4 iyMraj5qmLEJtewo/fSEFxmllqQ figure 2D ------- COMMENT: 0cfe17c3c16e2b0c 5 Er+JRCRz6pA1lzwVyPMjNKjmH9s figure 2d ------- COMMENT: 0cfe17c3c16e2b0c 6 Er+JRCRz6pA1lzwVyPMjNKjmH9s figure 2d ------- COMMENT: 0cfe17c3c16e2b0c 7 g9N6wSZd7VRBJ+13eFEfzt7Gjkc Figure 2B (comment: demonstrates robust arrest) ------- COMMENT: 0cfe17c3c16e2b0c 8 gNrUBVvlUgQ0RUmGTTQzdU1nHww 3b (comment: 80% 12 hours) ------- COMMENT: 0cfe17c3c16e2b0c 19 gDUBjv7rSYhQXJe5tm7V6j59HSc Figures S3B and S3C ------- COMMENT: 0cfe17c3c16e2b0c 20 isfuCy/4d/ajLdqf5/W2BSkhNUc Figures S3B and S3D ------- COMMENT: 0cfe17c3c16e2b0c 22 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 0cfe17c3c16e2b0c 23 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 0d06afc3089ee763 3 w5dp1FgBTAjXpufH8byReycYtTA ------- COMMENT: 0d06afc3089ee763 8 w5dp1FgBTAjXpufH8byReycYtTA ------- COMMENT: 0d06afc3089ee763 9 w5dp1FgBTAjXpufH8byReycYtTA ------- COMMENT: 0d06afc3089ee763 10 w5dp1FgBTAjXpufH8byReycYtTA ------- COMMENT: 0d06afc3089ee763 33 w5dp1FgBTAjXpufH8byReycYtTA ------- COMMENT: 0d06afc3089ee763 34 w5dp1FgBTAjXpufH8byReycYtTA ------- COMMENT: 0d06afc3089ee763 36 w5dp1FgBTAjXpufH8byReycYtTA ------- COMMENT: 0d1bc3ee794002e7 1 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 0d1bc3ee794002e7 3 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 0d1bc3ee794002e7 4 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 0d1bc3ee794002e7 5 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 0d1bc3ee794002e7 6 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 0d1bc3ee794002e7 7 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 0d1bc3ee794002e7 8 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 0d1bc3ee794002e7 9 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 0d1bc3ee794002e7 10 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 0d1bc3ee794002e7 11 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 0d1bc3ee794002e7 12 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 0d1bc3ee794002e7 13 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 0d1bc3ee794002e7 14 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 0d1bc3ee794002e7 15 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 0d1bc3ee794002e7 16 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 0d1bc3ee794002e7 17 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 0d1bc3ee794002e7 18 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 0d1bc3ee794002e7 19 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 0d1bc3ee794002e7 20 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 0d1bc3ee794002e7 21 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 0d1bc3ee794002e7 22 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 0d2e132805615e6d 1 wuRVmxdPVOvtt6sKZ0mM0x5sjt0 polarization, during conjugation, in shmoo fig1. We interpret the data as indicating that F-actin is first cor- rectly localized to the tip in all fus1 mutants, but is then re- distributed after a defective attempt to fuse. Thus, it is likely that Fus1 is required for the correct organization and stabilization of polarized F-actin at the tip, but is no ------- COMMENT: 0d2e132805615e6d 2 wMFhe4n51uZSb6PuLvL0IC4Vqfs (commment: polarization, in shmoo DNS) ------- COMMENT: 0d2e132805615e6d 3 awz0ZVnIb9sO8STJqXXA8AiQaeE (comment: DNS actin distributed in cytoplasm) ------- COMMENT: 0d2e132805615e6d 4 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 0d2e132805615e6d 5 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 0d2e132805615e6d 6 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 0d2e132805615e6d 7 o/4MvJcDP7YC/zKiTafiBp/Hmvw By immunofluorescence, these antibod- ies stained a single dot at the very tip of each cell in wild- type pre-zygotes (Fig. 2, C and D). ------- COMMENT: 0d2e132805615e6d 8 Xdfp5b2AmpjMBCqD37Qf+QhH9lQ Therefore, in a h90 mam2 strain, the P cells will attempt mating, but the M cells will be unable to respond due to the lack of the P-factor receptor, and so the cells will fail to initiate fusion ------- COMMENT: 0d2e132805615e6d 12 r2aiva2YPQqC3NSXCFg6HsV8cWo (slightly increased- In fact, more cells had staining at their tips than wild-type cells, probably indicating a prolonged attempt to conjugate, after which the protein delocalizes) ------- COMMENT: 0d2e132805615e6d 13 r2aiva2YPQqC3NSXCFg6HsV8cWo (slightly increased- In fact, more cells had staining at their tips than wild-type cells, probably indicating a prolonged attempt to conjugate, after which the protein delocalizes) ------- COMMENT: 0d2e132805615e6d 17 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: 0d2e132805615e6d 18 LAIWYkITQVdaCatJq+phtZLetmM In fact, more cells had staining at their tips than wild-type cells, probably indicating a prolonged attempt to conjugate, after which the protein delocalizes ------- COMMENT: 0d41be15f3286108 4 usZ05irR7NISphbENljjfraHOgI Fig1, data not shown cdc2-1w is called wee 2-1 in this paper. Wee cells enter mitosis at a small cell size compared to wild type cells and thus daughter cells are born smaller than wild type cells. Wee cells have similar cell cycle timing (doubling time) to wild type cells ------- COMMENT: 0d41be15f3286108 5 pSIJCAWa+z07GhfBmMXf1Gugxuc Table 1, Fig1 ------- COMMENT: 0d41be15f3286108 6 ANQZPRCxNLLXAxrTtUV4n8KWRpg Table 1, DNA replication initiated at low protein content ------- COMMENT: 0d41be15f3286108 7 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 0d41be15f3286108 8 LZzqEkJ3dw25k902OrbbAuWtvz0 Fig6, Table 2 cell size measure by protein content per cell, cells need to reach ~7pg/cell. This measurement is also the same for wild type cells which are small after nitrogen starvation (Fig5) ------- COMMENT: 0d41be15f3286108 10 JHKPidSqn9XHp9T2fLxrOFM9xHw Fig 2 This suggests that wee1-50 only has an indirect effect on the G1-S transition because when wee1-50 is shifted to the permissive temperature cells are still small, this is not affected by the presence of active wee1 and cells enter S-phase at the same size as they did at the restrictive temperature ------- COMMENT: 0d41be15f3286108 11 Cdnm7DZuiImo2dctHgq80TsxOfw Fig 2 This shows that wee1-50 has a direct effect on the G2-M transition because cells now at the permissive temperature (active wee1) detect that they are too small to enter mitosis and the G2/M transition is inhibited ------- COMMENT: 0d41be15f3286108 13 igLAQ3yRbMJFF80jj948gcpcmng Fig4, Table 2 cell size measure by protein content per cell, cells need to reach ~7pg/cell. This measurement is also the same for wild type cells which are small after spore germination (Fig3) ------- COMMENT: 0d41be15f3286108 14 pSIJCAWa+z07GhfBmMXf1Gugxuc Table 1, Fig1 ------- COMMENT: 0d638c12aef4db07 1 PkD68J8qYWHZud57ZiOF5tbsw4A Active against both cAMP and cGMP based on its ability to confer resistance to exogenous cyclic nucleotides. Fig. 1A, B ------- COMMENT: 0d638c12aef4db07 3 u9PARezQX9JmKSERR+kSmIor7Z4 Deletion of both cyr1/git2 and cgs2 produces cells that are hypersensitive to both exogenous cAMP and cGMP as these can activate PKA at low micromolar concentrations. Fig. 1A, B ------- COMMENT: 0d638c12aef4db07 13 PkD68J8qYWHZud57ZiOF5tbsw4A Active against both cAMP and cGMP based on its ability to confer resistance to exogenous cyclic nucleotides. Fig. 1A, B ------- COMMENT: 0de8787e35f61acb 2 Sqdty7MwbeKY61L7PknrEdi+u2k (comment: changed to decreased from abolished based on "H3-FLAG association with H3-H113D-HA was severely reduced) ------- COMMENT: 0de8787e35f61acb 9 bLBMxTZTp538FTBEYYDQylEcVMY fig. 2 ------- COMMENT: 0de8787e35f61acb 11 fIs74ZhcuzcJ/vCaJ2kEvMV/Bmo (comment: reduced binding of hht2-H113D) ------- COMMENT: 0de8787e35f61acb 12 RL/gFBYExVaXsf8ODCm6QVELjCk (comment: normal binding of hht2-H113D) ------- COMMENT: 0de8787e35f61acb 16 bTr+ovaTYdhejOfXPkbKauRXwL0 "Reciprocally, H3-H113D-HA association with wt H3 and H4 were severely reduced" ------- COMMENT: 0de8787e35f61acb 18 /P/W8l2Qf4H/MZj2tnIVFIfZ6t4 "Consistent with this, we found that the deletion of pcf1 is synthetic lethal with the deletion of hip1, the gene encoding one subunit of the fission yeast HIRA complex (S4A Fig). T" ------- COMMENT: 0de8787e35f61acb 19 YB8P6iR3pUC83ksr+rt77mqbPwM fig. 3 ------- COMMENT: 0de8787e35f61acb 20 Lm9j50rrmjDytcxDyg69epwbDSc fig. 3,4 ------- COMMENT: 0de8787e35f61acb 21 YB8P6iR3pUC83ksr+rt77mqbPwM fig. 3 ------- COMMENT: 0de8787e35f61acb 22 PCxwt9WzX5SP8qVJN7QM3kvuL8g fig. 3c ------- COMMENT: 0de8787e35f61acb 25 +HDTsE6dfRwBTS55KQY4gnjG9Wc ------- COMMENT: 0de8787e35f61acb 26 6z/jeQTi9gyp+wwZUVXIAorV69M fig. 6 ------- COMMENT: 0de8787e35f61acb 27 6z/jeQTi9gyp+wwZUVXIAorV69M fig. 6 ------- COMMENT: 0de8787e35f61acb 28 6z/jeQTi9gyp+wwZUVXIAorV69M fig. 6 ------- COMMENT: 0de8787e35f61acb 29 6z/jeQTi9gyp+wwZUVXIAorV69M fig. 6 ------- COMMENT: 0de8787e35f61acb 30 6z/jeQTi9gyp+wwZUVXIAorV69M fig. 6 ------- COMMENT: 0de8787e35f61acb 31 6z/jeQTi9gyp+wwZUVXIAorV69M fig. 6 ------- COMMENT: 0de8787e35f61acb 32 6z/jeQTi9gyp+wwZUVXIAorV69M fig. 6 ------- COMMENT: 0de8787e35f61acb 33 6z/jeQTi9gyp+wwZUVXIAorV69M fig. 6 ------- COMMENT: 0de8787e35f61acb 34 6z/jeQTi9gyp+wwZUVXIAorV69M fig. 6 ------- COMMENT: 0de8787e35f61acb 35 6z/jeQTi9gyp+wwZUVXIAorV69M fig. 6 ------- COMMENT: 0de8787e35f61acb 36 6z/jeQTi9gyp+wwZUVXIAorV69M fig. 6 ------- COMMENT: 0de8787e35f61acb 37 6z/jeQTi9gyp+wwZUVXIAorV69M fig. 6 ------- COMMENT: 0de8787e35f61acb 40 k+ygOqMv2atOhZayWVuUEg4QQjM fig. 7 ------- COMMENT: 0e16556e33d4d7d9 1 ioiVYLaMFGR7tJoyAIY9pATQipU Figure s2 (c); ------- COMMENT: 0e16556e33d4d7d9 2 RfJM+au3NU9+0toI1Z9K9v6Qr7w Figure 4d, 4e; Figure s3b; ------- COMMENT: 0e16556e33d4d7d9 3 f2FIk5kC+ubmihhmrIKCn65osQ0 ------- COMMENT: 0e16556e33d4d7d9 5 f2FIk5kC+ubmihhmrIKCn65osQ0 ------- COMMENT: 0e16556e33d4d7d9 7 f2FIk5kC+ubmihhmrIKCn65osQ0 ------- COMMENT: 0e16556e33d4d7d9 8 f2FIk5kC+ubmihhmrIKCn65osQ0 ------- COMMENT: 0e16556e33d4d7d9 9 f2FIk5kC+ubmihhmrIKCn65osQ0 ------- COMMENT: 0e38008c9ffaccbe 3 CLIhqL1HWUxUkQcHJ3RfCrLHfTE At high temperature (36C), the mutant protein appeared to bind PA nearly as well as the wild-type enzyme but exhibited a strongly decreased rate of catalysis. ------- COMMENT: 0e38008c9ffaccbe 4 wdZRO2h/7QHYJgpev+n7TfOmAkQ At the restrictive temperature of 36C, cells accumulate very large lipid droplets surrounded by the endoplasmic reticulum. These lipid droplets arise from persistent growth rather than fusion. ------- COMMENT: 0e38008c9ffaccbe 5 0xi0SWnOivIfilIiWmj5l2LHXbI Slow population growth rate can be rescued by supplementing the minimal medium with choline or ethanolamine, the precursors required for phospholipid biosynthesis through the de novo Kennedy pathway ------- COMMENT: 0e38008c9ffaccbe 6 1uksEI2FU2yZxPfNtmVZPMpw1TY (comment: Mutant cells grow normally in liquid rich medium) ------- COMMENT: 0e38008c9ffaccbe 7 j0JcmHKtdMyIFAabV/XkvfCdhQ0 (comment: The endoplasmic reticulum is wrapped around the abnormally large lipid droplets) ------- COMMENT: 0e38008c9ffaccbe 9 FgSj+/rbGRFCkq3auyt58iz9gTU (comment: Determined by thin layer chromatography, TLC) ------- COMMENT: 0e38008c9ffaccbe 11 Un8prddEndhNyinkxpppUOBz8ag (comment: Mutant cells grow normally in liquid minimal medium supplemented with choline.) ------- COMMENT: 0e38008c9ffaccbe 12 sCSe0Z0yxhH8w4WTZXMSYNytAd8 (comment: Mutant cells grow normally in liquid minimal medium supplemented with ethanolamine.) ------- COMMENT: 0e38008c9ffaccbe 13 FgSj+/rbGRFCkq3auyt58iz9gTU (comment: Determined by thin layer chromatography TLC) ------- COMMENT: 0e38008c9ffaccbe 14 FgSj+/rbGRFCkq3auyt58iz9gTU (comment: Determined by thin layer chromatography TLC) ------- COMMENT: 0e38008c9ffaccbe 15 FgSj+/rbGRFCkq3auyt58iz9gTU (comment: Determined by thin layer chromatography TLC) ------- COMMENT: 0e38008c9ffaccbe 16 vINC+fmF3WJPdEBn4C4JaMep7K4 (comment: permissive temperature for bbl1-9) ------- COMMENT: 0e38008c9ffaccbe 17 qbT5oTchTNrq79hbUziee1C1DSc (comment: restrictive temperature for bbl1-9) ------- COMMENT: 0e38008c9ffaccbe 18 qbT5oTchTNrq79hbUziee1C1DSc (comment: restrictive temperature for bbl1-9) ------- COMMENT: 0e38008c9ffaccbe 19 qbT5oTchTNrq79hbUziee1C1DSc (comment: restrictive temperature for bbl1-9) ------- COMMENT: 0e416dbb3b3521ed 5 A+7HJAKHGhdONd66MuwLFibfT/s (comment: legacy GI phenotype increased chronological lifespan) ------- COMMENT: 0e416dbb3b3521ed 6 yNvkU+Oi3zbuyHD35WySk7OHb0E (comment: legacy GI phenotype decreased chronological lifespan) ------- COMMENT: 0e416dbb3b3521ed 7 A+7HJAKHGhdONd66MuwLFibfT/s (comment: legacy GI phenotype increased chronological lifespan) ------- COMMENT: 0e416dbb3b3521ed 23 A+7HJAKHGhdONd66MuwLFibfT/s (comment: legacy GI phenotype increased chronological lifespan) ------- COMMENT: 0e416dbb3b3521ed 28 A+7HJAKHGhdONd66MuwLFibfT/s (comment: legacy GI phenotype increased chronological lifespan) ------- COMMENT: 0e416dbb3b3521ed 33 tBfCM9b65e5CY0tHr67DjUoR/AU (comment: same as maf1delta alone) ------- COMMENT: 0e4eff4ea2905045 1 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 0e4eff4ea2905045 2 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 0e4eff4ea2905045 3 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 0e4eff4ea2905045 4 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 0e4eff4ea2905045 5 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 0e4eff4ea2905045 6 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 0e4eff4ea2905045 7 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 0e4eff4ea2905045 8 80YDf0r5lO0lpWqzdGub4h2X3rI Fig. 3A, B and C ------- COMMENT: 0e4eff4ea2905045 9 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 0e4eff4ea2905045 10 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: 0e4eff4ea2905045 11 rQyWBgVUcT+nrknzIvg53MndLiI NPC clusters in nup132Δ nuclei coalesced into larger clusters that preferentially localized to the SPBs in mitosis. Fig. 3F ------- COMMENT: 0e4eff4ea2905045 12 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 0e4eff4ea2905045 13 cLKKHHathQCT1JZTf0i7RHzTu8A Fig. 5F ------- COMMENT: 0e4eff4ea2905045 14 cLKKHHathQCT1JZTf0i7RHzTu8A Fig. 5F ------- COMMENT: 0e4eff4ea2905045 15 cLKKHHathQCT1JZTf0i7RHzTu8A Fig. 5F ------- COMMENT: 0e4eff4ea2905045 16 cLKKHHathQCT1JZTf0i7RHzTu8A Fig. 5F ------- COMMENT: 0e4eff4ea2905045 17 cLKKHHathQCT1JZTf0i7RHzTu8A Fig. 5F ------- COMMENT: 0e4eff4ea2905045 18 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 0e4eff4ea2905045 19 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 0e4eff4ea2905045 20 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 0e4eff4ea2905045 21 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 0e4eff4ea2905045 22 xLld+U4XtO1Yq5XJ/vA+lPgihjU Fig. 6D ------- COMMENT: 0e4eff4ea2905045 23 xLld+U4XtO1Yq5XJ/vA+lPgihjU Fig. 6D ------- COMMENT: 0e4eff4ea2905045 24 xLld+U4XtO1Yq5XJ/vA+lPgihjU Fig. 6D ------- COMMENT: 0e4eff4ea2905045 25 xLld+U4XtO1Yq5XJ/vA+lPgihjU Fig. 6D ------- COMMENT: 0e4eff4ea2905045 26 /EKJDn1kiFepVVy1Uoj4agCFYLE Fig. 6E ------- COMMENT: 0e4eff4ea2905045 27 40dsOZBX0zP7v7acL+/gUJuhMRQ Continued NPC assembly in cdc25.22 arrested cells that have low Cdk1 activity suggests that unlike in metazoans, Cdk1 (SpCdc2) is not required for NPC assembly in S. pombe. ------- COMMENT: 0e630b9e0c38e790 2 7j8Xx0mzZbQeo3IUpgVLde6nu2g ------- COMMENT: 0e630b9e0c38e790 3 OJrLL1x2+GP4glthOWcdb07mmyo ------- COMMENT: 0e630b9e0c38e790 6 1sMD8sb3e8490cnQI3c/IMJWNa8 ------- COMMENT: 0e65902eb994aa19 1 pDOH0Q5cHAY/HBK6+pLhCJAowjI ------- COMMENT: 0e65902eb994aa19 4 snNLE40Ll0KzZ2oYsf97X0DhXhY ------- COMMENT: 0e65902eb994aa19 5 yvGFAIkAwjcw7ZBw3YHcbi6D0dU ------- COMMENT: 0e65902eb994aa19 6 Y0DfxWos22R/rZqM9PU4bAu1NG8 ------- COMMENT: 0e755de48a2d8865 3 dUCXeIcyBwqy4NW6FBkqZrfELNs When cells were grown in media containing nitro- gen sources, some of the nc1669􏰄 cells underwent mating (followed by meiosis), whereas WT cells never initiated mat- ing (Figure 3B, C) ------- COMMENT: 0e755de48a2d8865 4 dUCXeIcyBwqy4NW6FBkqZrfELNs When cells were grown in media containing nitro- gen sources, some of the nc1669􏰄 cells underwent mating (followed by meiosis), whereas WT cells never initiated mat- ing (Figure 3B, C) ------- COMMENT: 0e85b84df580612d 1 bqWG0fx0b/BfNF6bh3o5/NyCADw ------- COMMENT: 0e85b84df580612d 2 EWUtQwpFZvZUnu63/QPV2akXDMo ------- COMMENT: 0e85b84df580612d 3 T4Lc85AjJ+4olU9b/Wgf0QU7g2M ------- COMMENT: 0ed011499aba521b 12 s23UucwxJtN9W1FQc29v8Z2j/zk ------- COMMENT: 0eeecc7d59bfbbce 1 uYUhsk36maIhJEsXuPVov6begQY ers1 mutant is completely defective in the silencing of ura4 genes placed in the inner or outer repeats of cen1. ------- COMMENT: 0eeecc7d59bfbbce 3 chqO9v8YxBOAJVPn49UgDjiIv2g no defect in silencing of ura4 reporter genes placed at mat3M or tel2R, where RNAi- dependent mechanisms act redundantly with RNAi-independ- ent silencing mechanisms. ------- COMMENT: 0eeecc7d59bfbbce 4 chqO9v8YxBOAJVPn49UgDjiIv2g no defect in silencing of ura4 reporter genes placed at mat3M or tel2R, where RNAi- dependent mechanisms act redundantly with RNAi-independ- ent silencing mechanisms. ------- COMMENT: 0eeecc7d59bfbbce 5 TTq3mUv9UfpsIVr6SgyC4KmzTuo We found that ers1 cells display a defect in both H3 Lys9 dimethylation and trimethylation (both normalized for nucleosome density) at the endogenous dh and dg regions of the centromeric outer repeats (Fig. 1B). ------- COMMENT: 0eeecc7d59bfbbce 6 TTq3mUv9UfpsIVr6SgyC4KmzTuo We found that ers1 cells display a defect in both H3 Lys9 dimethylation and trimethylation (both normalized for nucleosome density) at the endogenous dh and dg regions of the centromeric outer repeats (Fig. 1B). ------- COMMENT: 0eeecc7d59bfbbce 7 TTq3mUv9UfpsIVr6SgyC4KmzTuo We found that ers1 cells display a defect in both H3 Lys9 dimethylation and trimethylation (both normalized for nucleosome density) at the endogenous dh and dg regions of the centromeric outer repeats (Fig. 1B). ------- COMMENT: 0eeecc7d59bfbbce 8 TTq3mUv9UfpsIVr6SgyC4KmzTuo We found that ers1 cells display a defect in both H3 Lys9 dimethylation and trimethylation (both normalized for nucleosome density) at the endogenous dh and dg regions of the centromeric outer repeats (Fig. 1B). ------- COMMENT: 0eeecc7d59bfbbce 9 XYme9Rn/odPE3YDwHDjCol2MiXM As shown in Fig. 1C, recruitment of RITS to both sites was abolished in ers1 cells. ------- COMMENT: 0eeecc7d59bfbbce 10 coBRqzMm6LQ7bYsg7YVyc0d1Q5s nalysis revealed robust accumulation of the centromeric dg transcript in ers1 cells (Fig. 2A). ------- COMMENT: 0eeecc7d59bfbbce 11 ZqeWtMvCsM6n4+fG2exoTjdPdig We observed an apparently complete defect in siRNA production (Fig. 2B). ------- COMMENT: 0f10611fa56323c0 29 4Ndaj1Kz4KN5LnxWGXShC9jqfgw Figure S3A Interestingly, we noticed during imaging that imp2-17A rlc1-mNG sid4-mNG cells displayed CR sliding events where the CR formed in the middle of the cell but then slid towards one cell tip (6/18 cells) (Fig. S3A). ------- COMMENT: 0f10611fa56323c0 30 kxyFjEbIrbJZBCiN6CLU+iyJelY Figure S3B-C ------- COMMENT: 0f10611fa56323c0 31 o61gNwtaxo2IdUA7cIfpLeZrB2k Figure S2A and C ------- COMMENT: 0f10611fa56323c0 32 SFoSQWBIunA2tplN62rJQJHhs0k Figure S2D-E ------- COMMENT: 0f10611fa56323c0 33 jITBoMUXWIqmLXPAln0QvYOdSCA Figure 4A-B ------- COMMENT: 0f10611fa56323c0 34 jITBoMUXWIqmLXPAln0QvYOdSCA Figure 4A-B ------- COMMENT: 0f10611fa56323c0 35 jITBoMUXWIqmLXPAln0QvYOdSCA Figure 4A-B ------- COMMENT: 0f10611fa56323c0 36 1C09U3SGlHvPkvhPIrOlXx1qV8w Figure 2G ------- COMMENT: 0f10611fa56323c0 37 SFoSQWBIunA2tplN62rJQJHhs0k Figure S2D-E ------- COMMENT: 0f10611fa56323c0 38 T/oLvUTKq89lA2NC7hBgwtDyrzE Figure S2F ------- COMMENT: 0f10611fa56323c0 39 5SLq4jjUZx4o2fqO4NJ9786gpN0 Figure S2C ------- COMMENT: 0f10611fa56323c0 40 kxyFjEbIrbJZBCiN6CLU+iyJelY Figure S3B-C ------- COMMENT: 0f10611fa56323c0 41 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 0f10611fa56323c0 42 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 0f10611fa56323c0 43 JpGz+lvWG0vfyEr/jdTh1hZkECw Figure 4. However, imp2-17E cells exhibited slower CR constriction. In fact, CR remnants remained in 62% of imp2-17E cells for the duration of our observation. ------- COMMENT: 0f10611fa56323c0 44 16/L1qPxU5XpakY0aXG37v1y1p0 Figure 3. Imp2-11E-mNG was not recruited to the CR later than Imp2-mNG but its peak accumulation was delayed compared to wildtype (Fig. 3A, B, and C) ------- COMMENT: 0f10611fa56323c0 45 f1ECGO6tG1vGxuDTJd0k+ad4nu8 Figure 2G However, mutation of 3 additional Cdk1 consensus sites abolished Imp2 phosphorylation by Cdk1 (Imp2-11A, Fig. 2C). ------- COMMENT: 0f10611fa56323c0 46 iXXxW1pNOykGnygV9GUUJ5UOpWA Figure 2G, H ------- COMMENT: 0f10611fa56323c0 47 Mc1Dl/8ePjG+W3BQIevC1vaEvqw Figure 2D ------- COMMENT: 0f10611fa56323c0 48 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 0f10611fa56323c0 49 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 0f10611fa56323c0 50 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 0f10611fa56323c0 51 GwgTp9YKZY5J84ue6Ea5M+DFcTE Figure 3. Imp2-11A-mNG was recruited to the CR earlier (ca. 4 minutes) than Imp2-mNG (Fig. 3A, B, and C). ------- COMMENT: 0f10611fa56323c0 52 WfAzzBzFCWJHlmP6VDkT1CKVFrg Figure SA and C ------- COMMENT: 0f10611fa56323c0 53 IKaaGS1fY2Tmcg/YOpMKR636UNg Figure S2D and F ------- COMMENT: 0f10611fa56323c0 54 SFoSQWBIunA2tplN62rJQJHhs0k Figure S2D-E ------- COMMENT: 0f10611fa56323c0 55 kxyFjEbIrbJZBCiN6CLU+iyJelY Figure S3B-C ------- COMMENT: 0f10611fa56323c0 56 SFoSQWBIunA2tplN62rJQJHhs0k Figure S2D-E ------- COMMENT: 0f10611fa56323c0 57 IKaaGS1fY2Tmcg/YOpMKR636UNg Figure S2D and F ------- COMMENT: 0f10611fa56323c0 58 o61gNwtaxo2IdUA7cIfpLeZrB2k Figure S2A and C ------- COMMENT: 0f10611fa56323c0 59 kxyFjEbIrbJZBCiN6CLU+iyJelY Figure S3B-C ------- COMMENT: 0f10611fa56323c0 60 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 0f10611fa56323c0 61 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 0f10611fa56323c0 62 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 0f10611fa56323c0 63 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 0f10611fa56323c0 68 /YjgqxTXk0IGbMcXyFsAA8D/074 Figure 2E ------- COMMENT: 0f10611fa56323c0 69 pwYo4qq3zpJvUEZsuqNj7EY7jzA Figure S4B ------- COMMENT: 0f10611fa56323c0 70 A1vhy0FYK+SoURt6KK+A31i4v2s Figure S4A ------- COMMENT: 0f10611fa56323c0 71 A1vhy0FYK+SoURt6KK+A31i4v2s Figure S4A ------- COMMENT: 0f10611fa56323c0 72 A1vhy0FYK+SoURt6KK+A31i4v2s Figure S4A ------- COMMENT: 0f10611fa56323c0 73 A1vhy0FYK+SoURt6KK+A31i4v2s Figure S4A ------- COMMENT: 0f10611fa56323c0 74 pwYo4qq3zpJvUEZsuqNj7EY7jzA Figure S4B ------- COMMENT: 0f10611fa56323c0 75 pwYo4qq3zpJvUEZsuqNj7EY7jzA Figure S4B ------- COMMENT: 0f10611fa56323c0 76 /yDHlwFNmyObnhljw0LCGyGxbAU Figure S2A-B there was no detectable defect in morphology or cell division in imp2-11A, imp2-11E, imp2-17E, imp2-28A or imp2-28E cells although some imp2-17A cells failed to separate, forming chains (Fig. S2A and B). ------- COMMENT: 0f10611fa56323c0 77 CUuhvl0QV28Id9dVbEOUmTYYRaE Figure S2A-B ------- COMMENT: 0f10611fa56323c0 78 CUuhvl0QV28Id9dVbEOUmTYYRaE Figure S2A-B ------- COMMENT: 0f10611fa56323c0 79 CUuhvl0QV28Id9dVbEOUmTYYRaE Figure S2A-B ------- COMMENT: 0f10611fa56323c0 80 CUuhvl0QV28Id9dVbEOUmTYYRaE Figure S2A-B ------- COMMENT: 0f10611fa56323c0 81 o61gNwtaxo2IdUA7cIfpLeZrB2k Figure S2A and C ------- COMMENT: 0f10611fa56323c0 82 o61gNwtaxo2IdUA7cIfpLeZrB2k Figure S2A and C ------- COMMENT: 0f10611fa56323c0 83 CUuhvl0QV28Id9dVbEOUmTYYRaE Figure S2A-B ------- COMMENT: 0f10611fa56323c0 86 aZ1Nkw7JS0Ts3WneOWmtPaPLhpU Figure S2A-C ------- COMMENT: 0f10611fa56323c0 87 2J9D8Gu1gJ1l1r77W0bxz1Wf8bc Figure 2F ------- COMMENT: 0f10611fa56323c0 96 6XWDaWBnMM2j/FpRqNw/vnk24E0 Figure 1A-C ------- COMMENT: 0f10611fa56323c0 97 6XWDaWBnMM2j/FpRqNw/vnk24E0 Figure 1A-C ------- COMMENT: 0f10611fa56323c0 98 6XWDaWBnMM2j/FpRqNw/vnk24E0 Figure 1A-C ------- COMMENT: 0f10611fa56323c0 99 6XWDaWBnMM2j/FpRqNw/vnk24E0 Figure 1A-C ------- COMMENT: 0f10611fa56323c0 100 6XWDaWBnMM2j/FpRqNw/vnk24E0 Figure 1A-C ------- COMMENT: 0f10611fa56323c0 101 6XWDaWBnMM2j/FpRqNw/vnk24E0 Figure 1A-C ------- COMMENT: 0f10611fa56323c0 102 6XWDaWBnMM2j/FpRqNw/vnk24E0 Figure 1A-C ------- COMMENT: 0f10611fa56323c0 103 6XWDaWBnMM2j/FpRqNw/vnk24E0 Figure 1A-C ------- COMMENT: 0f10611fa56323c0 104 6XWDaWBnMM2j/FpRqNw/vnk24E0 Figure 1A-C ------- COMMENT: 0f10611fa56323c0 105 6XWDaWBnMM2j/FpRqNw/vnk24E0 Figure 1A-C ------- COMMENT: 0f10611fa56323c0 106 6XWDaWBnMM2j/FpRqNw/vnk24E0 Figure 1A-C ------- COMMENT: 0f10611fa56323c0 107 6XWDaWBnMM2j/FpRqNw/vnk24E0 Figure 1A-C ------- COMMENT: 0f10611fa56323c0 108 6XWDaWBnMM2j/FpRqNw/vnk24E0 Figure 1A-C ------- COMMENT: 0f10611fa56323c0 109 6XWDaWBnMM2j/FpRqNw/vnk24E0 Figure 1A-C ------- COMMENT: 0f10611fa56323c0 110 6XWDaWBnMM2j/FpRqNw/vnk24E0 Figure 1A-C ------- COMMENT: 0f10611fa56323c0 111 6XWDaWBnMM2j/FpRqNw/vnk24E0 Figure 1A-C ------- COMMENT: 0f10611fa56323c0 112 6XWDaWBnMM2j/FpRqNw/vnk24E0 Figure 1A-C ------- COMMENT: 0f10611fa56323c0 113 Uamgj4cvvE6bz1iand0IspiDCec (comment: vw added extensions to link MF to BP & phase) ------- COMMENT: 0f10611fa56323c0 114 ce+IGV09aDEo3gUATq2PkJtbNaQ This phenotype suggests that phosphorylation of the 17 CK1 consensus sites in Imp2 promotes the medial anchoring of the CR on the membrane, possibly by stabilizing an unknown interaction involving the Imp2 IDR. ------- COMMENT: 0f10611fa56323c0 116 CUuhvl0QV28Id9dVbEOUmTYYRaE Figure S2A-B ------- COMMENT: 0f10611fa56323c0 117 iKdOBrKK2NQBX3r1EA3aMwaF4Kw Together these findings implicate Cdk1 in modulating the timing of Imp2 localization to the CR, and are consistent with the general theme of Cdk1 inhibiting cytokinesis until chromosome segregation is complete ------- COMMENT: 0f10611fa56323c0 118 E9tJzUnZz2VkyOLr5NoGvBf2NCA Imp2 was phosphorylated by Hhp1 in vitro, and mutation of the 15 identified and two more CK1 consensus sites eliminated this phosphorylation (Fig. 2B) ------- COMMENT: 0f70615b481efcb2 22 1U5SI+8O54HW99qgOskkf5Fknok ------- COMMENT: 0f72d4033c5d842f 1 gTx+YYkoln7gCfLAqBXbo8FJtUA Inter- estingly, Tti2 also pulls down Asa1, a protein previously identified in Rvb1 purifications from both S. cerevisiae and S. pombe (Shevchenko et al., 2008) ------- COMMENT: 0f72d4033c5d842f 2 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 0f72d4033c5d842f 3 QsiaU5jCCTKo1wFmIjMESbN3pYc In contrast, the AAA+ ATPases Rvb1 and Rvb2 were detected with poorer specificity and reproducibility, suggesting weaker or more transient interaction with Tti2. ------- COMMENT: 0f72d4033c5d842f 4 QsiaU5jCCTKo1wFmIjMESbN3pYc In contrast, the AAA+ ATPases Rvb1 and Rvb2 were detected with poorer specificity and reproducibility, suggesting weaker or more transient interaction with Tti2. ------- COMMENT: 0f72d4033c5d842f 5 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 0f72d4033c5d842f 6 7lBVJ+gwlDXwVFRTyjF9slThq9o Tti2 interacts with Tel2 and Tti1 almost stoichiometrically (Figures 1B and S1A; Table S1). ------- COMMENT: 0f72d4033c5d842f 7 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 0f72d4033c5d842f 8 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 0f72d4033c5d842f 9 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 0f72d4033c5d842f 10 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 0f72d4033c5d842f 11 7lBVJ+gwlDXwVFRTyjF9slThq9o Tti2 interacts with Tel2 and Tti1 almost stoichiometrically (Figures 1B and S1A; Table S1). ------- COMMENT: 0f72d4033c5d842f 12 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 0f72d4033c5d842f 37 ihJf9FlN+A/WQC85ddt6Sw63Tpw Western blotting followed by quantification of signal intensities showed that both the steady-state levels and the stability of Tra1, Tra2, and Tor1 decrease following Tti2 depletion (Figures 1C–1F). ------- COMMENT: 0f72d4033c5d842f 38 kVh1wRFOQ+7GDQ+S9tM5HAsu2v0 Depletion of each protein re- duces S. pombe viability and proliferation compared with control strains and culture conditions (Figures S1C and S1D). ------- COMMENT: 0f72d4033c5d842f 39 CJJC9n1y5kWn5fc7zO1YQAEDvZA (Figures S1C and S1D). ------- COMMENT: 0f72d4033c5d842f 40 CJJC9n1y5kWn5fc7zO1YQAEDvZA (Figures S1C and S1D). ------- COMMENT: 0f72d4033c5d842f 41 Nvjr6c54QHpltEwMLy7sh1l/qsw We used qRT-PCR to deter- mine the effect of TTT depletion on the expression of two sexual differentiation genes, ste11+ and mei2+. We observed that the mRNA levels of both genes increase upon depletion of Tel2, Tti1, and Tti2 compared with control strains and conditions (Figures S1E and S1F) ------- COMMENT: 0f72d4033c5d842f 42 CYGyfmq8oDBe5z2BQmI7Qs1NFtg (Figures S1E and S1F) ------- COMMENT: 0f72d4033c5d842f 43 CYGyfmq8oDBe5z2BQmI7Qs1NFtg (Figures S1E and S1F) ------- COMMENT: 0f72d4033c5d842f 44 GztDcVfpDkZAkGAaePOmM2neIGE Western blotting showed decreased phosphorylation of the ribosomal protein S6, a ca- nonical TORC1 substrate, following Tel2, Tti1, and Tti2 depletion (Figure S1G) ------- COMMENT: 0f72d4033c5d842f 45 2r6B7klAV262v/0FMEbaFUFn4bA (Figure S1G) ------- COMMENT: 0f72d4033c5d842f 46 2r6B7klAV262v/0FMEbaFUFn4bA (Figure S1G) ------- COMMENT: 0f72d4033c5d842f 50 C9q+H4ghbn4lvQCiMc6bjw1KPOE (Figure S4C) ------- COMMENT: 0f72d4033c5d842f 51 p3hVqgM62ak+mdo/V+PRCxSzQOw (Figure 3C) ------- COMMENT: 0f72d4033c5d842f 52 48y50V6UFEAxgDEFSaX0QpKxxfU (Figure S5) ------- COMMENT: 0f72d4033c5d842f 53 C9q+H4ghbn4lvQCiMc6bjw1KPOE (Figure S4C) ------- COMMENT: 0f72d4033c5d842f 54 C9q+H4ghbn4lvQCiMc6bjw1KPOE (Figure S4C) ------- COMMENT: 0f72d4033c5d842f 55 lcRgvO8GdGFjyZsZVxPB1rxdz9A (Figure 3D) ------- COMMENT: 0f72d4033c5d842f 56 lcRgvO8GdGFjyZsZVxPB1rxdz9A (Figure 3D) ------- COMMENT: 0f72d4033c5d842f 57 lcRgvO8GdGFjyZsZVxPB1rxdz9A (Figure 3D) ------- COMMENT: 0f72d4033c5d842f 58 p3hVqgM62ak+mdo/V+PRCxSzQOw (Figure 3C) ------- COMMENT: 0f72d4033c5d842f 59 p3hVqgM62ak+mdo/V+PRCxSzQOw (Figure 3C) ------- COMMENT: 0f72d4033c5d842f 60 FWVfBPBTWz+g8fW9SDd4EAW4qFo In contrast, Tti2 and Tti1 interaction with tra1+ does not change in the absence of Tel2 (Figure 2I), ------- COMMENT: 0f72d4033c5d842f 61 0ZGxLUXDVc5AwKdZZ04YoyMiciA (Figure 2H) ------- COMMENT: 0f72d4033c5d842f 89 WghzOSKf3pJyjub+laY2mH/LSzk Surprisingly, despite a strong decrease of Tor2 steady-state levels, its stability appears unaffected, even increasing 6 h after CHX treatment. It is possible that Tor2 is subjected to rapid turnover and compensa- tory mechanisms boosting its synthesis. ------- COMMENT: 0f72d4033c5d842f 94 7584mSlyG4GCDEMox2J5rU6B6A8 (Figure 6B) ------- COMMENT: 0f72d4033c5d842f 95 dLT8cRYlRsj+MbcAwmOxrlTF14g RNA-seq ------- COMMENT: 0f72d4033c5d842f 96 7584mSlyG4GCDEMox2J5rU6B6A8 (Figure 6B) ------- COMMENT: 0f72d4033c5d842f 97 7584mSlyG4GCDEMox2J5rU6B6A8 (Figure 6B) ------- COMMENT: 0f72d4033c5d842f 98 7584mSlyG4GCDEMox2J5rU6B6A8 (Figure 6B) ------- COMMENT: 0f72d4033c5d842f 99 7584mSlyG4GCDEMox2J5rU6B6A8 (Figure 6B) ------- COMMENT: 0f72d4033c5d842f 100 zCJ/1XkyOCsmSHg+1wObraisldo Figure 2C) Abolished interaction between Tti2 protein and tra1 mRNA ------- COMMENT: 0f72d4033c5d842f 107 tfndOe6cHbpgFfGVULhVJ7uZJ9I (Figure 3E) ------- COMMENT: 0f72d4033c5d842f 109 tfndOe6cHbpgFfGVULhVJ7uZJ9I (Figure 3E) ------- COMMENT: 0f72d4033c5d842f 110 tfndOe6cHbpgFfGVULhVJ7uZJ9I (Figure 3E) ------- COMMENT: 0f72d4033c5d842f 111 tfndOe6cHbpgFfGVULhVJ7uZJ9I (Figure 3E) ------- COMMENT: 0f72d4033c5d842f 112 tfndOe6cHbpgFfGVULhVJ7uZJ9I (Figure 3E) ------- COMMENT: 0f72d4033c5d842f 114 7584mSlyG4GCDEMox2J5rU6B6A8 (Figure 6B) ------- COMMENT: 0f72d4033c5d842f 115 7584mSlyG4GCDEMox2J5rU6B6A8 (Figure 6B) ------- COMMENT: 0f72d4033c5d842f 116 DaDH9HZgvHZSZhi1rLPj1bx/xK0 (Figure 6J) ------- COMMENT: 0f72d4033c5d842f 117 dLT8cRYlRsj+MbcAwmOxrlTF14g ------- COMMENT: 0f72d4033c5d842f 118 fVTSWEQaf5zMLt+5ayLyd+XCQeA (Figure 6H) ------- COMMENT: 0f72d4033c5d842f 119 fVTSWEQaf5zMLt+5ayLyd+XCQeA (Figure 6H) ------- COMMENT: 0f72d4033c5d842f 120 lcOl0qbfuXf8Y+2qGsd/IVrvu/s (figure 6F) ------- COMMENT: 0f72d4033c5d842f 121 dLT8cRYlRsj+MbcAwmOxrlTF14g RNA-seq ------- COMMENT: 0f72d4033c5d842f 122 fx/uz71O0c05o06fX38lPpHLjRo figure 6I ------- COMMENT: 0f72d4033c5d842f 123 fx/uz71O0c05o06fX38lPpHLjRo figure 6I ------- COMMENT: 0f72d4033c5d842f 125 LHYPi7f4v765/FZ3eTZ7K3ZwOqA (Figure 7A) ------- COMMENT: 0f72d4033c5d842f 127 LHYPi7f4v765/FZ3eTZ7K3ZwOqA (Figure 7A) ------- COMMENT: 0f72d4033c5d842f 134 fRW9I8oC4bEO8+Apn3rAMoIhiFw (comment: Abolished incorporation of Tra1 into SAGA complex) ------- COMMENT: 0f72d4033c5d842f 135 fRW9I8oC4bEO8+Apn3rAMoIhiFw (comment: Abolished incorporation of Tra1 into SAGA complex) ------- COMMENT: 0f72d4033c5d842f 136 fRW9I8oC4bEO8+Apn3rAMoIhiFw (comment: Abolished incorporation of Tra1 into SAGA complex) ------- COMMENT: 0f72d4033c5d842f 137 fRW9I8oC4bEO8+Apn3rAMoIhiFw (comment: Abolished incorporation of Tra1 into SAGA complex) ------- COMMENT: 0f72d4033c5d842f 138 fRW9I8oC4bEO8+Apn3rAMoIhiFw (comment: Abolished incorporation of Tra1 into SAGA complex) ------- COMMENT: 0f72d4033c5d842f 139 uyu4LAMQwKB4TowqdD+pSWZSpFw Altogether, our quantitative proteomic analyses indicate that Tti2, Tel2, and Tti1, together with Asa1, form a stable multi- meric complex that interacts with most PIKKs in S. pombe (Figure 1A) ------- COMMENT: 0f72d4033c5d842f 140 nBhTLTXAlYGvzIxpOZpltQtPkcI (Figure 1A) ------- COMMENT: 0f72d4033c5d842f 141 nBhTLTXAlYGvzIxpOZpltQtPkcI (Figure 1A) ------- COMMENT: 0f72d4033c5d842f 142 h5Iktf98WvXi5Ey3XIr1A9mVQNc Finally, our published tran- scriptomic analysis of tti2-CKO mutants showed that PIKK mRNA levels remain unaffected following Tti2 depletion (Fig- ureS1H) ------- COMMENT: 0f72d4033c5d842f 143 Ulp46lVT6SF0586woeqLAdQoIzg (Figure S2B). ------- COMMENT: 0f72d4033c5d842f 144 A0ZxhBW1f5dKSPoIchQ+yrLU/aw Tel2 does not localize to the nucleus in standard growth conditions (Figure S2B). ------- COMMENT: 0f72d4033c5d842f 145 xhZmilBhrE+NhF8s65ZxuJxcnk8 Conventional and qRT-PCR analyses revealed a specific enrichment of the tra1+ mRNA in RIPs of all three TTT subunits, Tel2, Tti1, and Tti2, compared with several negative controls (Figures 2A and 2B). To determine whether this interaction occurs cotranslationally, we then repeated Tti1 RIPs in cells treated either with puromycin, an inhibitor of translation elongation, or with EDTA, which disso- ciates ribosomes. Both treatments abolished Tti1 binding to tra1+ (Figure 2C). ------- COMMENT: 0f72d4033c5d842f 146 LyfDWKqoD6mq46Tk6zYuwzuvZgw (Figures 2A and 2B). To determine whether this interaction occurs cotranslationally, we then repeated Tti1 RIPs in cells treated either with puromycin, an inhibitor of translation elongation, or with EDTA, which disso- ciates ribosomes. Both treatments abolished Tti1 binding to tra1+ (Figure 2C). ------- COMMENT: 0f72d4033c5d842f 147 LyfDWKqoD6mq46Tk6zYuwzuvZgw (Figures 2A and 2B). To determine whether this interaction occurs cotranslationally, we then repeated Tti1 RIPs in cells treated either with puromycin, an inhibitor of translation elongation, or with EDTA, which disso- ciates ribosomes. Both treatments abolished Tti1 binding to tra1+ (Figure 2C). ------- COMMENT: 0f72d4033c5d842f 148 8JnVY8vTuD4lY1agrPGCd2nZjm4 Tti1 caused a strong decrease of Tti2 and Tel2 binding (Fig- ure 2J). ------- COMMENT: 0f72d4033c5d842f 149 JppBH+OwQ5tyIKDOmpbLvFukLKs We conclude that Tti1, and to a lesser extent Tti2, re- cruits TTT to nascent Tra1 polypeptides ------- COMMENT: 0f72d4033c5d842f 150 JppBH+OwQ5tyIKDOmpbLvFukLKs We conclude that Tti1, and to a lesser extent Tti2, re- cruits TTT to nascent Tra1 polypeptides ------- COMMENT: 0f79ae49e7ed5890 26 HJSuHLC3XiSnFHaEoZd/ZHxHR/Y (comment: increased unequal sister chromatid recombination) ------- COMMENT: 0f79ae49e7ed5890 32 HJSuHLC3XiSnFHaEoZd/ZHxHR/Y (comment: increased unequal sister chromatid recombination) ------- COMMENT: 0f79ae49e7ed5890 33 HJSuHLC3XiSnFHaEoZd/ZHxHR/Y (comment: increased unequal sister chromatid recombination) ------- COMMENT: 0f79ae49e7ed5890 35 dDduiuoB0ICSisyZ07UQatd0xC8 also ctp1,rec12,rad22,rti1,rad51,dmc1 (comment: increased unequal sister chromatid recombination) ------- COMMENT: 0f86c034b975c3a8 1 0/BQJ8fMGwHcs8VAWVBmcgZV73U (comment: inhibited by α-(hydroxymethyl)serine CHEBI:28187) ------- COMMENT: 0f9e5e33d1984340 1 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: 0f9e5e33d1984340 6 8z8dtzmMaPpqQ/2hfnLjaN14Gkw The phosphorylation of Psk1 and Rps602 was completely abolished in wat1Δ, wat1-17, and tor2-287 mutants after shifting the cells at non-permissive temperature (Fig. 4A, upper and middle panel). ------- COMMENT: 0f9e5e33d1984340 8 b8iNdDGBP3S0TKNJI1K9ZeU02jg cells were unable to grow on the plates containing canavanine (Fig. 4C). Interestingly, wat1Δ, wat1-17, and tor2-287 mutant cells exhibited resistance to canavanine (Fig. 4C) suggesting that disruption of wat1 leads to inactivation of the TORC1 pathway resulting in the defect in amino acid uptake ------- COMMENT: 0f9e5e33d1984340 9 NI8so/+84jW6pl8cADjwA6eTKOU Real-time quantitative PCR analysis revealed the up-regulation of transcripts of per1 and isp5 genes and down regulation of cat1 gene in wat1Δ, wat1-17, and tor2-287 mutant background at the non-permissive temperature (Fig. 4B), ------- COMMENT: 0f9e5e33d1984340 23 UynDirQKtwRawybm0zoBNCoXGhk wat1Δ and wat1-17 mutant cells were a little elongated with an average size of 18.5 μm (Fig. 3A and 3B). ------- COMMENT: 0f9e5e33d1984340 24 UynDirQKtwRawybm0zoBNCoXGhk wat1Δ and wat1-17 mutant cells were a little elongated with an average size of 18.5 μm (Fig. 3A and 3B). ------- COMMENT: 0f9e5e33d1984340 25 zWmutcNQUrSs03C1ughd/gBDbRE In contrast, under the same condition, the wat1Δ and wat1-17 mutant cells became shorter in size with a round morphology having an average cell length of 7.7 and 8.3 μm, respectively (Fig. 3A and 3B) ------- COMMENT: 0f9e5e33d1984340 26 Zg7cSDVjQ6Ji4vqP5AplLZTofLc Interestingly, the average cell size of tor-287 mutant cells also decreases from 18.2 μm at 25 ◦C to 7.0 μm at 36 ◦C (Fig. 3A and 3B ------- COMMENT: 0f9e5e33d1984340 27 UFD0ky0E9I3zy/Bxcl1pnI4iIAo having 1C and 2C DNA peak (Fig. 3C, upper panel) with a majority of cells having 1C DNA peak indicating a G1 arrest while only 2C DNA peak was observed in wild type cells grown under similar conditions ------- COMMENT: 0f9e5e33d1984340 29 IuYGyi7Vaul9zq6tG+fP+Uf/8Vs The tor2-287 mutant cells also showed a similar phenotype after shifting at 36 ◦C (Fig. 3C, upper panel), indicating the G1 arrest. ------- COMMENT: 0f9e5e33d1984340 30 8z8dtzmMaPpqQ/2hfnLjaN14Gkw The phosphorylation of Psk1 and Rps602 was completely abolished in wat1Δ, wat1-17, and tor2-287 mutants after shifting the cells at non-permissive temperature (Fig. 4A, upper and middle panel). ------- COMMENT: 0fa0811aad33bde1 2 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 0fa0811aad33bde1 3 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 0fa0811aad33bde1 4 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 0fa0811aad33bde1 5 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 0fa0811aad33bde1 6 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 0fa0811aad33bde1 7 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 0fa0811aad33bde1 8 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 0fa0811aad33bde1 9 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 0fa0811aad33bde1 10 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 0fa0811aad33bde1 11 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 0fa0811aad33bde1 12 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 0fa0811aad33bde1 13 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 0fa0811aad33bde1 14 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 0fa0811aad33bde1 15 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 0fa0811aad33bde1 16 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 0fa0811aad33bde1 17 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 0fa0811aad33bde1 18 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 0fa0811aad33bde1 19 4dlgqGM15n4dOW3uDiisqozPuLw Figure 8 ------- COMMENT: 0fa0811aad33bde1 20 4dlgqGM15n4dOW3uDiisqozPuLw Figure 8 ------- COMMENT: 0fa0811aad33bde1 21 VWRY+xIXdxFg+BJJ5YcgBRZgJC4 Figure 10 ------- COMMENT: 0fa0811aad33bde1 22 VWRY+xIXdxFg+BJJ5YcgBRZgJC4 Figure 10 ------- COMMENT: 0fa0811aad33bde1 23 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 0fa0811aad33bde1 24 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 0fa0811aad33bde1 25 crdk8+5Su6ZjOoDfvYBv9bmPLs0 Figure 5C ------- COMMENT: 0fa0811aad33bde1 26 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: 0fa0811aad33bde1 27 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: 0fa0811aad33bde1 28 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: 0fa0811aad33bde1 29 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: 0fa0811aad33bde1 30 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: 0fa0811aad33bde1 31 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: 0fa0811aad33bde1 32 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: 0fa0811aad33bde1 33 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: 0fa0811aad33bde1 34 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: 0fa0811aad33bde1 35 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: 0fa0811aad33bde1 36 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: 0fa0811aad33bde1 37 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: 0fa0811aad33bde1 38 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: 0fa0811aad33bde1 39 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: 0fa0811aad33bde1 40 AfC89UAsDp4dGF9yB/eHZAzeVik Figure 10 (comment: Manu: transfer to FYPO:0008075) ------- COMMENT: 0fa0811aad33bde1 41 xGQBTgLu2JVQpxHv2ov+HFx7orQ Figure 10 (comment: Manu: transfer to FYPO:0008075) ------- COMMENT: 0fa0811aad33bde1 42 crdk8+5Su6ZjOoDfvYBv9bmPLs0 Figure 5C ------- COMMENT: 0fa0811aad33bde1 43 crdk8+5Su6ZjOoDfvYBv9bmPLs0 Figure 5C ------- COMMENT: 0fa0811aad33bde1 44 VWRY+xIXdxFg+BJJ5YcgBRZgJC4 Figure 10 ------- COMMENT: 0fa0811aad33bde1 47 VWRY+xIXdxFg+BJJ5YcgBRZgJC4 Figure 10 ------- COMMENT: 0fa0811aad33bde1 48 4dlgqGM15n4dOW3uDiisqozPuLw Figure 8 ------- COMMENT: 0fa0811aad33bde1 49 /6bG8QKF2XfTe2akKFwVzhz2SBI Fig. 5 S2 ------- COMMENT: 0fa0811aad33bde1 50 /6bG8QKF2XfTe2akKFwVzhz2SBI Fig. 5 S2 ------- COMMENT: 0fa0811aad33bde1 51 /6bG8QKF2XfTe2akKFwVzhz2SBI Fig. 5 S2 ------- COMMENT: 0fa0811aad33bde1 52 /6bG8QKF2XfTe2akKFwVzhz2SBI Fig. 5 S2 ------- COMMENT: 0fa0811aad33bde1 53 /6bG8QKF2XfTe2akKFwVzhz2SBI Fig. 5 S2 ------- COMMENT: 0fa0811aad33bde1 54 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: 0fb2e4081135c7c3 3 sOcugTTOeXOSGQibbolFInyvc/w Live cell imaging revealed that GFP-Lac1 and Lag1-GFP remain localized at the ER in the absence of Lag1 or Lac1 respectively, indicating that their ER localizations are not interdependent. ------- COMMENT: 0fb2e4081135c7c3 4 ZvRG94RDI7zRDOQ5r56LiPB9FxI Additionally, the pattern of complex sphingolipids in Lac1-depleted cells shows a strong accumulation of IPC and the appearance of new bands that might correspond to different IPC species ------- COMMENT: 0fb2e4081135c7c3 5 7ZpZ5F7bYGJ8uUU8iCsRJw+2bmw fig 5 b. We detected an accumulation of PHS and sphingoid bases-1-phosphate levels (PHS-1P or DHS-1P) ------- COMMENT: 0fb2e4081135c7c3 14 CybsFDW9ceiXi0mwwSfig6nDd5s Fig 3. Live cell imaging revealed that GFP-Lac1 and Lag1-GFP remain localized at the ER in the absence of Lag1 or Lac1 respectively, indicating that their ER localizations are not interdependent. ------- COMMENT: 0fb2e4081135c7c3 15 CybsFDW9ceiXi0mwwSfig6nDd5s Fig 3. Live cell imaging revealed that GFP-Lac1 and Lag1-GFP remain localized at the ER in the absence of Lag1 or Lac1 respectively, indicating that their ER localizations are not interdependent. ------- COMMENT: 0fd8899c3eff64d1 4 QGsIrdKYh0PwBVE+nvrH2Enokkg figure 1 a ------- COMMENT: 0fd8899c3eff64d1 5 QGsIrdKYh0PwBVE+nvrH2Enokkg figure 1 a ------- COMMENT: 0fd8899c3eff64d1 6 JnPnJ5Rv1ruYqhB23BkYgUwtwlw figure 2 a ------- COMMENT: 0fd8899c3eff64d1 7 JnPnJ5Rv1ruYqhB23BkYgUwtwlw figure 2 a ------- COMMENT: 0fd8899c3eff64d1 8 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 0fd8899c3eff64d1 9 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 0fd8899c3eff64d1 10 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 0fd8899c3eff64d1 11 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 0fd8899c3eff64d1 12 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 0fd8899c3eff64d1 13 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 0fd9142a80a1f4c8 1 fN4H3R+7T2MV+69l366Rfw0TAro (Figure 2A The resulting strain, rgf1􏰃, showed a slow growth pattern at 28°C ------- COMMENT: 0fd9142a80a1f4c8 2 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 0fd9142a80a1f4c8 3 hlT4GluMGLcLqtrjXg5Xzc0eVjQ Figure 2B regardless of the growth temperature 30 –35% of the cells were lysed, ------- COMMENT: 0fd9142a80a1f4c8 4 hlT4GluMGLcLqtrjXg5Xzc0eVjQ Figure 2B regardless of the growth temperature 30 –35% of the cells were lysed, ------- COMMENT: 0fd9142a80a1f4c8 5 hlT4GluMGLcLqtrjXg5Xzc0eVjQ Figure 2B regardless of the growth temperature 30 –35% of the cells were lysed, ------- COMMENT: 0fd9142a80a1f4c8 7 xDZEpzPy+ji8VbgllRY2ZVf7wGQ (comment: positive regulation) ------- COMMENT: 0fd9142a80a1f4c8 8 khjHqrkX4ktRPwPHxNGVVikif58 this being consistent with the idea that rgf1􏰃 could act in the same pathway as rho1􏰃 (Figure 4A). Figure 5. The amount of active Rho1p increased considerably in the strain overexpressing Rgf1p compared with the wild- type strain. Moreover, only a minor amount of GTP-Rho1p was detected in the strain lacking Rgf1p. ------- COMMENT: 0fd9142a80a1f4c8 9 79E9BhMR0fL5bg6HvMMhkTbEgHM Figure 3A, As shown in Figure 3A, the rgf1 mutants showed a defect in actin organization in that they organized actin patches mostly at one end of the cell only ------- COMMENT: 0fd9142a80a1f4c8 10 sdrpkkaXsm0iCJvVrYNVy3JewZM Figure 3A (comment: at cell division site) ------- COMMENT: 0fd9142a80a1f4c8 11 29Jr612c0Oa6eGpB9wbo4mcSp9U (Figure 3B) 55% of cdc10-129 cells displayed bipolar growth, whereas only 4% of cdc10-129 rgf1􏰁 cells were bipolar ------- COMMENT: 0fd9142a80a1f4c8 12 xDZEpzPy+ji8VbgllRY2ZVf7wGQ (comment: positive regulation) ------- COMMENT: 0fd9142a80a1f4c8 13 hlT4GluMGLcLqtrjXg5Xzc0eVjQ Figure 2B regardless of the growth temperature 30 –35% of the cells were lysed, ------- COMMENT: 0fd9142a80a1f4c8 14 xDZEpzPy+ji8VbgllRY2ZVf7wGQ (comment: positive regulation) ------- COMMENT: 0fd9142a80a1f4c8 15 6LJHb/COHtR1U6EwPs5aOSFH24w Figure 4A, the Csp hypersensitivity of the rgf1delta mutant was suppressed by rho1delta in .... None of the other genes was able to suppress the hypersensitivity of rgf1􏰁; ------- COMMENT: 0fd9142a80a1f4c8 16 6LJHb/COHtR1U6EwPs5aOSFH24w Figure 4A, the Csp hypersensitivity of the rgf1delta mutant was suppressed by rho1delta in .... None of the other genes was able to suppress the hypersensitivity of rgf1􏰁; ------- COMMENT: 0fd9142a80a1f4c8 17 6LJHb/COHtR1U6EwPs5aOSFH24w Figure 4A, the Csp hypersensitivity of the rgf1delta mutant was suppressed by rho1delta in .... None of the other genes was able to suppress the hypersensitivity of rgf1􏰁; ------- COMMENT: 0fd9142a80a1f4c8 18 6LJHb/COHtR1U6EwPs5aOSFH24w Figure 4A, the Csp hypersensitivity of the rgf1delta mutant was suppressed by rho1delta in .... None of the other genes was able to suppress the hypersensitivity of rgf1􏰁; ------- COMMENT: 0fd9142a80a1f4c8 19 6LJHb/COHtR1U6EwPs5aOSFH24w Figure 4A, the Csp hypersensitivity of the rgf1delta mutant was suppressed by rho1delta in .... None of the other genes was able to suppress the hypersensitivity of rgf1􏰁; ------- COMMENT: 0fd9142a80a1f4c8 20 6LJHb/COHtR1U6EwPs5aOSFH24w Figure 4A, the Csp hypersensitivity of the rgf1delta mutant was suppressed by rho1delta in .... None of the other genes was able to suppress the hypersensitivity of rgf1􏰁; ------- COMMENT: 0fd9142a80a1f4c8 21 VavapWaleUq6IdKXD37ts7bBvv4 Figure 4c Lack of Rga1p produces small colonies and the cells show a swollen, multiseptated or branched shape; a pheno- type similar to that seen in cells in which Rho1p is excessively activated ------- COMMENT: 0fd9142a80a1f4c8 23 fN4H3R+7T2MV+69l366Rfw0TAro (Figure 2A The resulting strain, rgf1􏰃, showed a slow growth pattern at 28°C ------- COMMENT: 0fd9142a80a1f4c8 24 hKdHDFck0RYbAKt+7FsZvlJxETM rga1􏰁 cells were severely impaired for growth, whereas rgf1􏰁rga1􏰁 exhibited a better growth pat- tern and resembled rgf1􏰁 cells. ------- COMMENT: 0fd9142a80a1f4c8 25 JZOfNDRn6Zd0dc8wd8pdp9lXD1k (comment: rescue of multiseptate, swollen) ------- COMMENT: 0fd9142a80a1f4c8 26 EvXidCOsdyzG2hVFbVHmjl1D4IM (comment: increased gtp-bound gtpase, active) ------- COMMENT: 0fd9142a80a1f4c8 27 AWFurQuvKIFb9BcDdRIOLkblk/I (comment: decreased gtp-bound gtpase, inactive) ------- COMMENT: 0fd9142a80a1f4c8 29 hlT4GluMGLcLqtrjXg5Xzc0eVjQ Figure 2B regardless of the growth temperature 30 –35% of the cells were lysed, ------- COMMENT: 0fd9142a80a1f4c8 30 6jjLWU08l2gDhvDswkOWIu+m8YI (Figure 6B) GS activity increased during rgf1􏰃 overex- pression. This activity was fourfold higher than that ob- served in the wild-type strain ------- COMMENT: 0fd9142a80a1f4c8 31 Qw2dm6LVjZtl67IkSW4NS/z5gOw Figure 7A, only a moderate expres- sion of bgs4􏰃 restored growth of an rgf1􏰁 mutant in the presence of the antifungal agent ------- COMMENT: 0fd9142a80a1f4c8 32 l5qI6LyhATFyk660mveZKDzvf+E Figure 7A ------- COMMENT: 0fd9142a80a1f4c8 33 l5qI6LyhATFyk660mveZKDzvf+E Figure 7A ------- COMMENT: 0fd9142a80a1f4c8 34 l5qI6LyhATFyk660mveZKDzvf+E Figure 7A ------- COMMENT: 0fd9142a80a1f4c8 35 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: 0fd9142a80a1f4c8 36 xDZEpzPy+ji8VbgllRY2ZVf7wGQ (comment: positive regulation) ------- COMMENT: 0fd9142a80a1f4c8 37 grbNxnkHMXhMA0tMYnH18oouX9o Figure 7C ------- COMMENT: 0fd9142a80a1f4c8 38 xCbKZnANhFixEzZd8f2L384Cqpk Figure 8A ------- COMMENT: 0fd9142a80a1f4c8 39 xCbKZnANhFixEzZd8f2L384Cqpk Figure 8A ------- COMMENT: 0fd9142a80a1f4c8 40 xCbKZnANhFixEzZd8f2L384Cqpk Figure 8A ------- COMMENT: 0fd9142a80a1f4c8 41 xCbKZnANhFixEzZd8f2L384Cqpk Figure 8A ------- COMMENT: 0ff28f78db735798 1 yfineiH7bph2L97Jtaif4bklUro Fig. S5. ------- COMMENT: 0ff28f78db735798 2 yfineiH7bph2L97Jtaif4bklUro Fig. S5. ------- COMMENT: 0ff28f78db735798 3 6L5wiKVfLAxEYoX1lOqe+PIUbZs Fig. 5. ------- COMMENT: 0ff28f78db735798 4 yfineiH7bph2L97Jtaif4bklUro Fig. S5. ------- COMMENT: 0ff28f78db735798 7 6L5wiKVfLAxEYoX1lOqe+PIUbZs Fig. 5. ------- COMMENT: 0ff28f78db735798 10 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: 0ff28f78db735798 11 joUktSPUGXl1ezDJB/dL25jTXFA Figure 1 ------- COMMENT: 0ff28f78db735798 26 EK3cJaIpApHaZfDj/il8fbVV8BY fig S2 ------- COMMENT: 0ff28f78db735798 27 EK3cJaIpApHaZfDj/il8fbVV8BY fig S2 ------- COMMENT: 0ff28f78db735798 28 EK3cJaIpApHaZfDj/il8fbVV8BY fig S2 ------- COMMENT: 0ff28f78db735798 29 EK3cJaIpApHaZfDj/il8fbVV8BY fig S2 ------- COMMENT: 0ff28f78db735798 30 EK3cJaIpApHaZfDj/il8fbVV8BY fig S2 ------- COMMENT: 0ff28f78db735798 32 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 0ff28f78db735798 34 6L5wiKVfLAxEYoX1lOqe+PIUbZs Fig. 5. ------- COMMENT: 0ff28f78db735798 35 c+5olb9UnOwyrgNa8ErP3j+48Ys Microscopic examination revealed that Δsty1Δppr10 cells were highly elongated compared to WT, Δppr10, and Δsty1 cells (Fig. 5B), suggesting that progression from G2 into mitosis was impaired in Δsty1Δppr10 cells. ------- COMMENT: 0ff28f78db735798 36 6L5wiKVfLAxEYoX1lOqe+PIUbZs Fig. 5. ------- COMMENT: 0ff28f78db735798 37 T80nv/kU1M+gHcGqjQVunse3UtM Fig. 7b (comment: partial rescue - still loses viabiltiy at 48 hours) ------- COMMENT: 10192b215ce8c3bb 5 zpx4+x1YFUzthDwwl2oNicRd8SM (comment: they say periphery in the text but it has TM domains) ------- COMMENT: 10192b215ce8c3bb 41 vLXm3axEQhMtyQIXG/v62pFaz7A ------- COMMENT: 1021271c94244356 2 QpsfwpBGbozxU7K+VRdQOqBEm/k fig 2 (comment: maximum 3 septa) ------- COMMENT: 1021271c94244356 5 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig 2 ------- COMMENT: 1021271c94244356 6 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: 1021271c94244356 9 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig 2 ------- COMMENT: 1021271c94244356 10 QpsfwpBGbozxU7K+VRdQOqBEm/k fig 2 (comment: maximum 3 septa) ------- COMMENT: 1021271c94244356 11 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 1021271c94244356 12 8ElcFm0iVC9m3lWJL6vWwZK/ykI (Fig. 4, B and C) ------- COMMENT: 1021271c94244356 13 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: 1021271c94244356 14 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: 1021271c94244356 15 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: 1021271c94244356 16 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: 1021271c94244356 17 nl3QucUMr6YCbSbI9ZYy6Db0aWM fig 7a ------- COMMENT: 1021271c94244356 18 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: 1021271c94244356 19 QkVEGeQKr73/DJHB4+w2qQJd/Tk fig 7b ------- COMMENT: 1021271c94244356 22 NX6v/toclXh9222PH8RUlVRwEAI DNS ------- COMMENT: 1021271c94244356 23 NX6v/toclXh9222PH8RUlVRwEAI DNS ------- COMMENT: 1021271c94244356 24 NX6v/toclXh9222PH8RUlVRwEAI DNS ------- COMMENT: 1021271c94244356 25 NX6v/toclXh9222PH8RUlVRwEAI DNS ------- COMMENT: 1021271c94244356 26 NX6v/toclXh9222PH8RUlVRwEAI DNS ------- COMMENT: 1021271c94244356 27 R7MzjqsFmWNn3c8ZqjvX74qUrJg "exhibited well- defined, normal actin rings" ------- COMMENT: 1021271c94244356 29 ecHGfhzmbNUQLYn2SL4T/NXOGSQ fig7c ------- COMMENT: 1021271c94244356 30 jKE0HAk4aLf+MD8iL/svYPS8rHQ fig 8 ------- COMMENT: 1021271c94244356 31 jKE0HAk4aLf+MD8iL/svYPS8rHQ fig 8 ------- COMMENT: 1028f9ea656cb67b 1 zm+yEq0CMIECE+ePCcPEohu7ZcA ------- COMMENT: 1028f9ea656cb67b 2 zm+yEq0CMIECE+ePCcPEohu7ZcA ------- COMMENT: 1028f9ea656cb67b 3 zm+yEq0CMIECE+ePCcPEohu7ZcA ------- COMMENT: 1028f9ea656cb67b 4 zm+yEq0CMIECE+ePCcPEohu7ZcA ------- COMMENT: 1028f9ea656cb67b 5 zm+yEq0CMIECE+ePCcPEohu7ZcA ------- COMMENT: 1028f9ea656cb67b 6 zm+yEq0CMIECE+ePCcPEohu7ZcA ------- COMMENT: 1028f9ea656cb67b 7 zm+yEq0CMIECE+ePCcPEohu7ZcA ------- COMMENT: 1028f9ea656cb67b 8 7i8RJEmpc9z+1Bs6uI7D+hd70uQ ------- COMMENT: 1028f9ea656cb67b 9 7i8RJEmpc9z+1Bs6uI7D+hd70uQ ------- COMMENT: 1028f9ea656cb67b 10 7i8RJEmpc9z+1Bs6uI7D+hd70uQ ------- COMMENT: 1028f9ea656cb67b 11 7i8RJEmpc9z+1Bs6uI7D+hd70uQ ------- COMMENT: 1028f9ea656cb67b 12 7i8RJEmpc9z+1Bs6uI7D+hd70uQ ------- COMMENT: 1028f9ea656cb67b 13 7i8RJEmpc9z+1Bs6uI7D+hd70uQ ------- COMMENT: 1028f9ea656cb67b 14 7i8RJEmpc9z+1Bs6uI7D+hd70uQ ------- COMMENT: 1028f9ea656cb67b 15 1SYF3MXTiPr/Vkncr+5qrB9c+Hk Figure 1–figure supplement 1A ------- COMMENT: 1028f9ea656cb67b 16 1SYF3MXTiPr/Vkncr+5qrB9c+Hk Figure 1–figure supplement 1A ------- COMMENT: 1028f9ea656cb67b 17 U0WM1eS13q4YX1rpmNh7pjnxWWQ Figure 1–figure supplement 1B/Figure 1–figure supplement 1C ------- COMMENT: 1028f9ea656cb67b 18 2dM1sPskFQoyBXDam4kbthlUXAM (comment: PRE element) Figure 1–figure supplement 1B/Figure 1–figure supplement 1C ------- COMMENT: 1028f9ea656cb67b 19 7eoSZs5zDy1frJztcZQN9Qq1x9s Figure 1A and Figure 1–figure supplement 1D- E) ------- COMMENT: 1028f9ea656cb67b 20 7eoSZs5zDy1frJztcZQN9Qq1x9s Figure 1A and Figure 1–figure supplement 1D- E) ------- COMMENT: 1028f9ea656cb67b 26 mbg4b2WsmLcXDvsLdqeBCRUM4EQ Figure 1A and Figure 1–figure supplement 1D- E) (I think it is correct to describe as an enzyme regulator (MF, because it increases processivity.) ------- COMMENT: 1028f9ea656cb67b 27 uwUdXmIWI6gJAcAq95d+3jmqWrk Figure 1B and Figure 1–figure supplement 1D-E (I think it is correct to describe as an enzyme regulator (MF, because it increases processivity.) ------- COMMENT: 1028f9ea656cb67b 45 Dygvz0IEwP6twsKHK/jsDJvx/Qc figure 2 B ------- COMMENT: 1028f9ea656cb67b 46 Dygvz0IEwP6twsKHK/jsDJvx/Qc figure 2 B ------- COMMENT: 1028f9ea656cb67b 47 Dygvz0IEwP6twsKHK/jsDJvx/Qc figure 2 B ------- COMMENT: 1028f9ea656cb67b 48 Dygvz0IEwP6twsKHK/jsDJvx/Qc figure 2 B ------- COMMENT: 1028f9ea656cb67b 49 Dygvz0IEwP6twsKHK/jsDJvx/Qc figure 2 B ------- COMMENT: 1028f9ea656cb67b 50 Dygvz0IEwP6twsKHK/jsDJvx/Qc figure 2 B ------- COMMENT: 1028f9ea656cb67b 51 Dygvz0IEwP6twsKHK/jsDJvx/Qc figure 2 B ------- COMMENT: 1028f9ea656cb67b 52 Dygvz0IEwP6twsKHK/jsDJvx/Qc figure 2 B ------- COMMENT: 1028f9ea656cb67b 53 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: 1028f9ea656cb67b 54 Dygvz0IEwP6twsKHK/jsDJvx/Qc figure 2 B ------- COMMENT: 1028f9ea656cb67b 55 Dygvz0IEwP6twsKHK/jsDJvx/Qc figure 2 B ------- COMMENT: 1028f9ea656cb67b 56 7KuJdm8Bv/ToFcj/uGrst9N3HK0 ADD DOMAIN WHEN SO TERM AVAILABLE Figure 2A and Figure 2–figure supplement 1B-E ------- COMMENT: 1028f9ea656cb67b 58 pa3oNYsR2IluyZ2DzSK42/F3MLw Figure 2A and Figure 2–figure supplement 1B-E ------- COMMENT: 1028f9ea656cb67b 59 pa3oNYsR2IluyZ2DzSK42/F3MLw Figure 2A and Figure 2–figure supplement 1B-E ------- COMMENT: 102974a067180de1 2 vOo+uLzmCgjnGDS7iyUHCjylRbg ------- COMMENT: 102974a067180de1 4 vOo+uLzmCgjnGDS7iyUHCjylRbg ------- COMMENT: 102974a067180de1 5 Bf6OvVGudw+QJoL1qtcttYr5vyk (comment: increased during response to DNA damage by MMS or ionizing radiation; dissociates during response to HU) ------- COMMENT: 104f1a2efcecbd00 1 R1PlorS2Fa3tTcKJDqwPBLKYRcc (comment: they show it is GPI anchored, the specified residue is predicted) ------- COMMENT: 104f1a2efcecbd00 4 wd/ZbuqMwrYnhSdbyjisJhJW4ng Fig. 6 ------- COMMENT: 104f1a2efcecbd00 5 bohDSRdUFOhYmkhMTmcmaQGBR7Y Fig. 4 ------- COMMENT: 104f1a2efcecbd00 7 AkAp7jwyGJsQ4hWXIT7SxY703o4 Fig. 6a ------- COMMENT: 104f1a2efcecbd00 19 bohDSRdUFOhYmkhMTmcmaQGBR7Y Fig. 4 ------- COMMENT: 104f1a2efcecbd00 20 Ko4m6PzXiWMB6cw4zmoAGQCFf60 Fig. 8b ------- COMMENT: 104f1a2efcecbd00 21 Ct58LPl/ddAJl19Ncg0fpsGfSJc Fig. 9 ------- COMMENT: 10569b018029d625 1 oe1ea/0xJsZ2wNT3WFkHDQ2iURo (comment: Loss of cia1+ led to a lethal phenotype) ------- COMMENT: 1068c5e5c8e4b6df 8 DlZMxd5fxIeaE859j5MImm/QyCs Figure 3A ------- COMMENT: 1068c5e5c8e4b6df 9 XW/3Xg1nbqft385xDlcJFGwQoBY Figure 4E ------- COMMENT: 1068c5e5c8e4b6df 10 JyIlPthb667qMqefzq+EUEQJZV4 Figure 4 ------- COMMENT: 1068c5e5c8e4b6df 11 NyIzb6ABkGjkoqwkePzKDMQ4l6g Figure 2C ------- COMMENT: 1068c5e5c8e4b6df 12 yd2vvzgvv6GQhTq1ro85PRIB8I8 Figure 4A ------- COMMENT: 1068c5e5c8e4b6df 13 DlZMxd5fxIeaE859j5MImm/QyCs Figure 3A ------- COMMENT: 1068c5e5c8e4b6df 14 dyLgiShGltUyyGKyhcE9Rtp691w Figure 4C ------- COMMENT: 1068c5e5c8e4b6df 15 YdQWQywuGqkbnv6waTImUZTHSL0 Figure 4E-F ------- COMMENT: 1068c5e5c8e4b6df 16 yd2vvzgvv6GQhTq1ro85PRIB8I8 Figure 4A ------- COMMENT: 1068c5e5c8e4b6df 17 b0IKJ9c3OMCl4uXTm+Uugsun578 Figure 4A ------- COMMENT: 1068c5e5c8e4b6df 18 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 1068c5e5c8e4b6df 19 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 1068c5e5c8e4b6df 20 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 1068c5e5c8e4b6df 21 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 1068c5e5c8e4b6df 22 yd2vvzgvv6GQhTq1ro85PRIB8I8 Figure 4A ------- COMMENT: 1068c5e5c8e4b6df 24 hV8dFE6lthaEQgbtWD0nqRE5JZE Figure 1A-B ------- COMMENT: 1068c5e5c8e4b6df 33 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 1068c5e5c8e4b6df 44 NyIzb6ABkGjkoqwkePzKDMQ4l6g Figure 2C ------- COMMENT: 10ab5798d63d82a1 1 T6YsJHx1v5CLDSYFeluStDm6IX4 we generated a pho1∆ pho4∆ strain that grew as well as wild-type on YES agar medium (Fig. 3A). ------- COMMENT: 10ab5798d63d82a1 2 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 10ab5798d63d82a1 3 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 10ab5798d63d82a1 4 SBoQ7rTKFabBbhkBlIg27ITJvm4 Protein content of supernant medium from wild-type cells that were starved for phosphate for 12 hours was analyzed by UHPLC-MS ------- COMMENT: 10ab5798d63d82a1 5 SBoQ7rTKFabBbhkBlIg27ITJvm4 Protein content of supernant medium from wild-type cells that were starved for phosphate for 12 hours was analyzed by UHPLC-MS ------- COMMENT: 10ab5798d63d82a1 6 SBoQ7rTKFabBbhkBlIg27ITJvm4 Protein content of supernant medium from wild-type cells that were starved for phosphate for 12 hours was analyzed by UHPLC-MS ------- COMMENT: 10ab5798d63d82a1 7 SBoQ7rTKFabBbhkBlIg27ITJvm4 Protein content of supernant medium from wild-type cells that were starved for phosphate for 12 hours was analyzed by UHPLC-MS ------- COMMENT: 10ab5798d63d82a1 8 SBoQ7rTKFabBbhkBlIg27ITJvm4 Protein content of supernant medium from wild-type cells that were starved for phosphate for 12 hours was analyzed by UHPLC-MS ------- COMMENT: 10ab5798d63d82a1 9 SBoQ7rTKFabBbhkBlIg27ITJvm4 Protein content of supernant medium from wild-type cells that were starved for phosphate for 12 hours was analyzed by UHPLC-MS ------- COMMENT: 10ab5798d63d82a1 10 SBoQ7rTKFabBbhkBlIg27ITJvm4 Protein content of supernant medium from wild-type cells that were starved for phosphate for 12 hours was analyzed by UHPLC-MS ------- COMMENT: 10ab5798d63d82a1 11 SBoQ7rTKFabBbhkBlIg27ITJvm4 Protein content of supernant medium from wild-type cells that were starved for phosphate for 12 hours was analyzed by UHPLC-MS ------- COMMENT: 10ab5798d63d82a1 12 SBoQ7rTKFabBbhkBlIg27ITJvm4 Protein content of supernant medium from wild-type cells that were starved for phosphate for 12 hours was analyzed by UHPLC-MS ------- COMMENT: 10ab5798d63d82a1 13 SBoQ7rTKFabBbhkBlIg27ITJvm4 Protein content of supernant medium from wild-type cells that were starved for phosphate for 12 hours was analyzed by UHPLC-MS ------- COMMENT: 10ab5798d63d82a1 14 SBoQ7rTKFabBbhkBlIg27ITJvm4 Protein content of supernant medium from wild-type cells that were starved for phosphate for 12 hours was analyzed by UHPLC-MS ------- COMMENT: 10ab5798d63d82a1 15 SBoQ7rTKFabBbhkBlIg27ITJvm4 Protein content of supernant medium from wild-type cells that were starved for phosphate for 12 hours was analyzed by UHPLC-MS ------- COMMENT: 10ab5798d63d82a1 16 SBoQ7rTKFabBbhkBlIg27ITJvm4 Protein content of supernant medium from wild-type cells that were starved for phosphate for 12 hours was analyzed by UHPLC-MS ------- COMMENT: 10ab5798d63d82a1 17 FaW4s3NfxTdODkzrYCcac6U3uoQ Fig. 3C (comment: Assayed activity using medium supernatant) ------- COMMENT: 10ab5798d63d82a1 22 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 10ab5798d63d82a1 23 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 10ab5798d63d82a1 24 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 10ab5798d63d82a1 25 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 10ab5798d63d82a1 26 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 10ab5798d63d82a1 27 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 10ab5798d63d82a1 28 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 10ab5798d63d82a1 29 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 10ab5798d63d82a1 30 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 10ab5798d63d82a1 31 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 10ab5798d63d82a1 32 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 10ab5798d63d82a1 33 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 10ab5798d63d82a1 34 GuGl3VtIcJtgcmvUObcbZIHfsw4 Fig. 6 We conclude that Pho1 acid phosphatase and extracellular 5'-nucleotidase activities, which are induced during acute phosphate starvation, are not important for survival during chronic phosphate starvation. It is likely that the lifespan-shortening effects of ablating Pho7 reflect its role in promoting the expression of several hundred other fission yeast genes (8). ------- COMMENT: 10ab5798d63d82a1 35 JmeRYu/TINhpIyf/ZHme/yzpAvY A list of the 13 most abundant secreted proteins detected in the medium of phosphate- starved fission yeast cells is compiled in Fig. 2A ------- COMMENT: 10ab5798d63d82a1 36 JmeRYu/TINhpIyf/ZHme/yzpAvY A list of the 13 most abundant secreted proteins detected in the medium of phosphate- starved fission yeast cells is compiled in Fig. 2A ------- COMMENT: 10ab5798d63d82a1 37 JmeRYu/TINhpIyf/ZHme/yzpAvY A list of the 13 most abundant secreted proteins detected in the medium of phosphate- starved fission yeast cells is compiled in Fig. 2A ------- COMMENT: 10ab5798d63d82a1 38 JmeRYu/TINhpIyf/ZHme/yzpAvY A list of the 13 most abundant secreted proteins detected in the medium of phosphate- starved fission yeast cells is compiled in Fig. 2A ------- COMMENT: 10ab5798d63d82a1 39 JmeRYu/TINhpIyf/ZHme/yzpAvY A list of the 13 most abundant secreted proteins detected in the medium of phosphate- starved fission yeast cells is compiled in Fig. 2A ------- COMMENT: 10ab5798d63d82a1 40 JmeRYu/TINhpIyf/ZHme/yzpAvY A list of the 13 most abundant secreted proteins detected in the medium of phosphate- starved fission yeast cells is compiled in Fig. 2A ------- COMMENT: 10ab5798d63d82a1 41 JmeRYu/TINhpIyf/ZHme/yzpAvY A list of the 13 most abundant secreted proteins detected in the medium of phosphate- starved fission yeast cells is compiled in Fig. 2A ------- COMMENT: 10ab5798d63d82a1 42 JmeRYu/TINhpIyf/ZHme/yzpAvY A list of the 13 most abundant secreted proteins detected in the medium of phosphate- starved fission yeast cells is compiled in Fig. 2A ------- COMMENT: 10ab5798d63d82a1 43 JmeRYu/TINhpIyf/ZHme/yzpAvY A list of the 13 most abundant secreted proteins detected in the medium of phosphate- starved fission yeast cells is compiled in Fig. 2A ------- COMMENT: 10ab5798d63d82a1 44 JmeRYu/TINhpIyf/ZHme/yzpAvY A list of the 13 most abundant secreted proteins detected in the medium of phosphate- starved fission yeast cells is compiled in Fig. 2A ------- COMMENT: 10ab5798d63d82a1 45 JmeRYu/TINhpIyf/ZHme/yzpAvY A list of the 13 most abundant secreted proteins detected in the medium of phosphate- starved fission yeast cells is compiled in Fig. 2A ------- COMMENT: 10ab5798d63d82a1 46 JmeRYu/TINhpIyf/ZHme/yzpAvY A list of the 13 most abundant secreted proteins detected in the medium of phosphate- starved fission yeast cells is compiled in Fig. 2A ------- COMMENT: 10ab5798d63d82a1 47 JmeRYu/TINhpIyf/ZHme/yzpAvY A list of the 13 most abundant secreted proteins detected in the medium of phosphate- starved fission yeast cells is compiled in Fig. 2A ------- COMMENT: 10ab5798d63d82a1 51 uoyYmFqVHb317Z+3PXdLKSAUhWk figure 5 It is apparent that Efn1 contributes a greater share of secreted 5'-nucleotidase activity against AMP, GMP, and UMP compared to Efn2. ------- COMMENT: 10ab5798d63d82a1 52 C1vIhzWkTlEyB5+DdGCwejvDxZc figure 5 The Efn1:Efn2 activity ratio is 5.0 for AMP, 6.3 for GMP, and 4.1 for UMP (Fig. 5) However, the Efn1:Efn2 activity ratio for CMP hydrolysis is 1.3, suggesting that Efn2 displays a greater selectivity for CMP than does Efn1. ------- COMMENT: 10e451ad98a96256 1 PxGYNeSxrV5PD+XotSQQNBxfnSo fig 1a ------- COMMENT: 10e451ad98a96256 2 PxGYNeSxrV5PD+XotSQQNBxfnSo fig 1a ------- COMMENT: 10e451ad98a96256 3 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: 10e451ad98a96256 4 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: 10e451ad98a96256 5 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: 10e451ad98a96256 6 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: 10e451ad98a96256 7 RsQLY8G9NdIPJglUlTq9fDb41hI fig 1B As shown in Fig. 1B, when Mph1-KD was expressed from pREP41, it caused a weak growth inhibition, which was partially relieved by deletion of mad2+ or mph1+, indicating that expression of Mph1-KD from pREP41 caused a weak delay in mitotic progression as well as a growth defect for a reason unrelated to the checkpoint ac- tivation. We speculate that partially degraded Mph1-KD pro- teins (Fig. S2B) might be toxic to some extent. ------- COMMENT: 10e451ad98a96256 8 RsQLY8G9NdIPJglUlTq9fDb41hI fig 1B As shown in Fig. 1B, when Mph1-KD was expressed from pREP41, it caused a weak growth inhibition, which was partially relieved by deletion of mad2+ or mph1+, indicating that expression of Mph1-KD from pREP41 caused a weak delay in mitotic progression as well as a growth defect for a reason unrelated to the checkpoint ac- tivation. We speculate that partially degraded Mph1-KD pro- teins (Fig. S2B) might be toxic to some extent. ------- COMMENT: 10e451ad98a96256 9 9s0j1N1WUQwkVqyJ3IW7v3g3rzE We also examined localization of Mad2. Mad2 remained on kinetochores in more than 80% of the cells, indicating that the spindle checkpoint was kept active (Fig. S1 B and C) ------- COMMENT: 10e451ad98a96256 11 RsQLY8G9NdIPJglUlTq9fDb41hI fig 1B As shown in Fig. 1B, when Mph1-KD was expressed from pREP41, it caused a weak growth inhibition, which was partially relieved by deletion of mad2+ or mph1+, indicating that expression of Mph1-KD from pREP41 caused a weak delay in mitotic progression as well as a growth defect for a reason unrelated to the checkpoint ac- tivation. We speculate that partially degraded Mph1-KD pro- teins (Fig. S2B) might be toxic to some extent. ------- COMMENT: 10e451ad98a96256 12 lV++t1pp2Oron9uyELtjyxJLz80 As shown in Fig. 3A, ex- pression of Mph1-Ndc80-GFP from pREP81 caused an arrest in the wild-type background. ------- COMMENT: 10e451ad98a96256 13 lV++t1pp2Oron9uyELtjyxJLz80 As shown in Fig. 3A, ex- pression of Mph1-Ndc80-GFP from pREP81 caused an arrest in the wild-type background. ------- COMMENT: 10e451ad98a96256 14 JWN5tkKS0o0w+Pw2eVqGyUoEhKM It failed to cause an arrest in a strain lacking mad2+, indicating that the arrest was due to activation of the spindle checkpoint. ------- COMMENT: 10e451ad98a96256 18 9/6FIRrSHqIzDRd6udU5MdTb6GI When Mad2 was turned on, the index of the chromosome condensation gradually increased from 0 to more than 50%. Binucleate cells, which passed through anaphase, however, did not increase. These results indicated that when Mad2 was turned on, the cells, which were initially at the boundary of G2/M, were arrested before anaphase (Fig. 5B). ------- COMMENT: 1116101d850ce3e6 1 /dbWsD2/BLp1ZwlFrF6EbafVz+o Figure 1A ------- COMMENT: 1116101d850ce3e6 2 /dbWsD2/BLp1ZwlFrF6EbafVz+o Figure 1A ------- COMMENT: 1116101d850ce3e6 3 u5NstTm9+D7SOLo8XByDovu2OUk figure 1A ------- COMMENT: 1116101d850ce3e6 7 j0sFwPNhhHJ/lr9E3wNigYLUxSM figure 1D ------- COMMENT: 1116101d850ce3e6 8 j0sFwPNhhHJ/lr9E3wNigYLUxSM figure 1D ------- COMMENT: 1116101d850ce3e6 9 j0sFwPNhhHJ/lr9E3wNigYLUxSM figure 1D ------- COMMENT: 1116101d850ce3e6 11 /uY9l8p2BBIQO2aGF/iylgViW30 (comment: minor) ------- COMMENT: 1116101d850ce3e6 13 c/YxC+xXSzP9VK2nPX0DA6ZG8bI Figure 5A ------- COMMENT: 1116101d850ce3e6 14 r7t89y4TbzsI+sIhgJ+ACWxm8mc Figures 3 and 6 ------- COMMENT: 1116101d850ce3e6 15 r7t89y4TbzsI+sIhgJ+ACWxm8mc Figures 3 and 6 ------- COMMENT: 1116101d850ce3e6 16 r7t89y4TbzsI+sIhgJ+ACWxm8mc Figures 3 and 6 ------- COMMENT: 1116101d850ce3e6 17 r7t89y4TbzsI+sIhgJ+ACWxm8mc Figures 3 and 6 ------- COMMENT: 1116101d850ce3e6 21 /dbWsD2/BLp1ZwlFrF6EbafVz+o Figure 1A ------- COMMENT: 118900a57ae7a3da 7 MoRGtV2jdD24ni4JeTZ9ylt523Q (Fig. 2A). We found that Ssp2-GFP mainly localized in the nucleus both in glucose-starved cells and in cells grown in glucose-rich medium ------- COMMENT: 118900a57ae7a3da 12 ngm7oxRE7IEGIxxtMhL4ZUDqhLg (comment: ssp2 inferred from mutant phenotype) ------- COMMENT: 118900a57ae7a3da 19 bbIyAxp3b8a2RNJwAFBUh4yKrfg (comment: same as ssp2delta alone) ------- COMMENT: 118900a57ae7a3da 20 bbIyAxp3b8a2RNJwAFBUh4yKrfg (comment: same as ssp2delta alone) ------- COMMENT: 118900a57ae7a3da 21 bbIyAxp3b8a2RNJwAFBUh4yKrfg (comment: same as ssp2delta alone) ------- COMMENT: 118900a57ae7a3da 27 bbIyAxp3b8a2RNJwAFBUh4yKrfg (comment: same as ssp2delta alone) ------- COMMENT: 118900a57ae7a3da 31 bbIyAxp3b8a2RNJwAFBUh4yKrfg (comment: same as ssp2delta alone) ------- COMMENT: 118900a57ae7a3da 34 bbIyAxp3b8a2RNJwAFBUh4yKrfg (comment: same as ssp2delta alone) ------- COMMENT: 118900a57ae7a3da 64 t7ckOFk14foigW9Y2pM7Kshgvow (comment: OK, this MF is a stretch, but based on everything we know phenotypes, export of phosphorylated -typical TF regulation, ortholog etc, I'm confident these phenotypes can be used with curator knowledge to infer this._ ------- COMMENT: 11b0f6081ab49c11 1 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 11b0f6081ab49c11 5 CdEXpM80T5OrSiO7etYm9wGSc/g Figure 3 ------- COMMENT: 11b0f6081ab49c11 6 Ah7zZ0nP55Gm7YAYa2W9k9udv7M Figure 3b-g ------- COMMENT: 11b0f6081ab49c11 7 crHzDXEK4HH/w0ONC6E3LxL4OMc Figure 3b–g,i ------- COMMENT: 11b0f6081ab49c11 8 W1XzDANZzb8bGxnOu7l9qvqj2fg Figure 3b–g,i ------- COMMENT: 11b0f6081ab49c11 9 y4TxIOsRlwzzWcwUSR0V4S2hfv4 Figure 3fg ------- COMMENT: 11b0f6081ab49c11 10 y4TxIOsRlwzzWcwUSR0V4S2hfv4 Figure 3fg ------- COMMENT: 11b0f6081ab49c11 12 PphzBVSKnmf9a1U1ra9HfTBwIMU Figure 4, table 1 ------- COMMENT: 11b0f6081ab49c11 13 gm1Px94b5UBdjxqoHSq63NZdxOQ table 1 ------- COMMENT: 11b0f6081ab49c11 14 gm1Px94b5UBdjxqoHSq63NZdxOQ table 1 ------- COMMENT: 11b0f6081ab49c11 17 1Q75bToClluyPKBy2asEwqlWAtQ figure 5a ------- COMMENT: 11b0f6081ab49c11 18 AxNMnR0/DOitC/Ip0KKe8h28SvE figure 5bc ------- COMMENT: 11b0f6081ab49c11 19 +8LvJaDE3WzlBy29MD9KgIbBggc figure 5e ------- COMMENT: 11dc4403d7d5569a 6 fbl0fHqq97AxxbzxKdsUYnGYaj8 ------- COMMENT: 11dc4403d7d5569a 9 fbl0fHqq97AxxbzxKdsUYnGYaj8 ------- COMMENT: 11e42e4be40ab42d 1 FfydqVjDnLq8MVTSLVJ7bnJv7WY Analysis of nrm1+ transcript abundance in temperature-sensitive cdc25-22 cells arrested in G2/M and, subsequently, released into the cell cycle revealed that nrm1+, like the well-established G1-specific targetscdc18+ and ste9+, is a G1-specific transcript (Figure 6D). ------- COMMENT: 11e42e4be40ab42d 2 olKoAILBEJg7lICQz8DAFAeJK7E nrm1D mutants exhibited elevated levels of these MBF-dependent transcripts throughout the cell cycle (cdc22+, Figure 6F; cdc18+ and ste9+, data not shown). We conclude that SpNrm1 is essential for repression of MBF-regulated genes outside of the G1 phase and that both Res2 and the SpNrm1 are required for periodic expression of G1-specific transcripts. I ------- COMMENT: 11e42e4be40ab42d 3 olKoAILBEJg7lICQz8DAFAeJK7E nrm1D mutants exhibited elevated levels of these MBF-dependent transcripts throughout the cell cycle (cdc22+ , Figure 6F; cdc18+ and ste9+, data not shown). We conclude that SpNrm1 is essential for repression of MBF-regulated genes outside of the G1 phase and that both Res2 and the SpNrm1 are required for periodic expression of G1-specific transcripts. I ------- COMMENT: 11e42e4be40ab42d 4 olKoAILBEJg7lICQz8DAFAeJK7E nrm1D mutants exhibited elevated levels of these MBF-dependent transcripts throughout the cell cycle (cdc22+, Figure 6F; cdc18+ and ste9+, data not shown). We conclude that SpNrm1 is essential for repression of MBF-regulated genes outside of the G1 phase and that both Res2 and the SpNrm1 are required for periodic expression of G1-specific transcripts. I ------- COMMENT: 11f8c49a46c7964f 35 0EH6Ty3JeGK3c4WWW9ZtDQ1E1cU ------- COMMENT: 1203df6a6f3957f8 2 BJ79CCdIs9uCpO29r+29Uejo1Lo Figure 1A ------- COMMENT: 1203df6a6f3957f8 4 BJ79CCdIs9uCpO29r+29Uejo1Lo Figure 1A ------- COMMENT: 1203df6a6f3957f8 6 0cn96O+PUHoWOuDN0dy+AYGNHtA Figure 2C ------- COMMENT: 1203df6a6f3957f8 8 0cn96O+PUHoWOuDN0dy+AYGNHtA Figure 2C ------- COMMENT: 1203df6a6f3957f8 10 rPkHhGKyh7sT+VewKwHPwIwPKaY ------- COMMENT: 1203df6a6f3957f8 15 wFoDbeBDCUTevS781Vedd+eKOB4 Figure 4A ------- COMMENT: 1203df6a6f3957f8 17 +lGokglJTKvk0vvTDRIpieifdEo Figure 4B ------- COMMENT: 1203df6a6f3957f8 19 E976lZIYm66ZLYYlrNZnQbfj1Yg (comment: Question could be required for, or upstream spindle checkpoint, but it could cause a problem which preceds the point where it is possible to activate the checkpoint?BUT...alp14 and mad2 in same pathway and overexpression of mad2 cannot resuce defect of double/single mutant) ------- COMMENT: 1203df6a6f3957f8 21 vJ0lZoCU+xgbQ5kUDF7MF3PXbT8 (comment: cut2 levels were reduced in alp14 mutant) ------- COMMENT: 1203df6a6f3957f8 25 XXVqUCu6+GbI8AiJX38SElR2cg4 (comment: dependent on mitotic spindle) ------- COMMENT: 1203df6a6f3957f8 27 /dbWsD2/BLp1ZwlFrF6EbafVz+o Figure 1A ------- COMMENT: 1203df6a6f3957f8 28 /dbWsD2/BLp1ZwlFrF6EbafVz+o Figure 1A ------- COMMENT: 1203df6a6f3957f8 29 Z6tEIFMvHePTiPE8ZKnvdnLWmOI Figure 1C ------- COMMENT: 1203df6a6f3957f8 30 Z6tEIFMvHePTiPE8ZKnvdnLWmOI Figure 1C ------- COMMENT: 1203df6a6f3957f8 31 Z6tEIFMvHePTiPE8ZKnvdnLWmOI Figure 1C ------- COMMENT: 1203df6a6f3957f8 33 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 1203df6a6f3957f8 34 dzd/Va8UlboX80pVd3iixpPGq6w Figure 3A, (comment: rapid loss of viability) ------- COMMENT: 1203df6a6f3957f8 35 Vg36Pb3XgZZRHTX+vSWCnG6rMUM Figure 3B ------- COMMENT: 1203df6a6f3957f8 36 bhSWc6l3XUxembpY1KdSchuFnEo Figure 3F ------- COMMENT: 12398ff6230de6a3 4 M8CrM5g3MC2gPTk201YV02S6RTs (comment: Very high levels of diploidization in minimal medium) ------- COMMENT: 12398ff6230de6a3 5 jSFJIQ8mW1iLLw9XHIl0gVdQ39Q (comment: Very prominent in minimal medium due to the lack of the Kennedy pathway precursors) ------- COMMENT: 12398ff6230de6a3 7 jSFJIQ8mW1iLLw9XHIl0gVdQ39Q (comment: Very prominent in minimal medium due to the lack of the Kennedy pathway precursors) ------- COMMENT: 12398ff6230de6a3 8 jSFJIQ8mW1iLLw9XHIl0gVdQ39Q (comment: Very prominent in minimal medium due to the lack of the Kennedy pathway precursors) ------- COMMENT: 12398ff6230de6a3 9 PkPzw1kHa2sGkmiMFTDVnBsoW68 (comment: Sub-lethal phenotype, with only 10% of expected double mutants recovered.) ------- COMMENT: 12398ff6230de6a3 13 KfV0by3L0APkYTLaQNP3eVqRoH4 ------- COMMENT: 12398ff6230de6a3 14 KfV0by3L0APkYTLaQNP3eVqRoH4 ------- COMMENT: 12398ff6230de6a3 15 KfV0by3L0APkYTLaQNP3eVqRoH4 ------- COMMENT: 12398ff6230de6a3 16 KfV0by3L0APkYTLaQNP3eVqRoH4 ------- COMMENT: 12398ff6230de6a3 17 KfV0by3L0APkYTLaQNP3eVqRoH4 ------- COMMENT: 12398ff6230de6a3 18 KfV0by3L0APkYTLaQNP3eVqRoH4 ------- COMMENT: 12398ff6230de6a3 38 pt3KXJ0bXXhd1OAnYI1UP8VuoiU Severe buckling of the mitotic spindle was observed in 31% of cells, resulting in bowshaped nuclear intermediates during anaphase (Fig. 4D). It took longer for these nuclei to divide, and they often formed daughter nuclei of unequal sizes (Fig. S4E) ------- COMMENT: 12398ff6230de6a3 39 pt3KXJ0bXXhd1OAnYI1UP8VuoiU Severe buckling of the mitotic spindle was observed in 31% of cells, resulting in bowshaped nuclear intermediates during anaphase (Fig. 4D). It took longer for these nuclei to divide, and they often formed daughter nuclei of unequal sizes (Fig. S4E) ------- COMMENT: 124f1f372bfd6615 1 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 124f1f372bfd6615 2 abSHEdR60LPHk1u9wsXlum1rnF8 Fig. 5B and 5C ------- COMMENT: 124f1f372bfd6615 3 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 124f1f372bfd6615 4 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 124f1f372bfd6615 5 abSHEdR60LPHk1u9wsXlum1rnF8 Fig. 5B and 5C ------- COMMENT: 124f1f372bfd6615 6 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 124f1f372bfd6615 7 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 124f1f372bfd6615 8 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 124f1f372bfd6615 9 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 124f1f372bfd6615 10 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 124f1f372bfd6615 11 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 124f1f372bfd6615 12 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 124f1f372bfd6615 13 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 124f1f372bfd6615 14 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 124f1f372bfd6615 15 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 124f1f372bfd6615 16 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 124f1f372bfd6615 17 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 124f1f372bfd6615 18 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 124f1f372bfd6615 19 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 124f1f372bfd6615 20 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 124f1f372bfd6615 21 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 124f1f372bfd6615 22 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 124f1f372bfd6615 23 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 124f1f372bfd6615 24 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 124f1f372bfd6615 25 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 124f1f372bfd6615 26 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 124f1f372bfd6615 27 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 124f1f372bfd6615 28 u6D20lgQZ0sAQCCCgdTgjuncfNI we demonstrated that the Swi2-Swi5 complex promotes Rad51-driven strand invasion in vitro ------- COMMENT: 124f1f372bfd6615 29 u6D20lgQZ0sAQCCCgdTgjuncfNI we demonstrated that the Swi2-Swi5 complex promotes Rad51-driven strand invasion in vitro ------- COMMENT: 124f1f372bfd6615 30 s+LWTOwJCxIX7dsvWD79IPkgwn4 Rad54 further stimulates the activation of strand invasion of Rad51 by the Swi2-Swi5 complex. ------- COMMENT: 124f1f372bfd6615 31 wLMvdTmM5+3xwMqORrKM50aotaI the Swi6-binding site was required for the mat2-P donor choice ------- COMMENT: 124f1f372bfd6615 32 xfccGVxrdVY5t2fM5EuZB8fPpro the AT-hook motifs were required for the mat3-M donor choice. ------- COMMENT: 124f1f372bfd6615 33 u6D20lgQZ0sAQCCCgdTgjuncfNI we demonstrated that the Swi2-Swi5 complex promotes Rad51-driven strand invasion in vitro ------- COMMENT: 12517e47bd2ece51 1 gwrkUZyRyc6od4yI24nHkrdrxHU We observed that loss of Bhd1 and Ypt71, but not Ypt7, resulted in increased TORC1 activity, as determined by an increase in Rps6 and p70 S6K phosphorylation levels when cells were deprived of amino acids (Fig. 5a, compare lanes 1, 3, 5 and 7). ------- COMMENT: 12517e47bd2ece51 4 gwrkUZyRyc6od4yI24nHkrdrxHU We observed that loss of Bhd1 and Ypt71, but not Ypt7, resulted in increased TORC1 activity, as determined by an increase in Rps6 and p70 S6K phosphorylation levels when cells were deprived of amino acids (Fig. 5a, compare lanes 1, 3, 5 and 7). ------- COMMENT: 12517e47bd2ece51 5 h+rN06tu68IEh3peN2uiGFj4YMw Strains lacking Ypt7 (ypt7Δ, bhd1Δ ypt7Δ, ypt71Δ ypt7Δ) displayed a significant growth defect in the low amino-acid condition (EMM plates supplemented with low concentration of amino acids) compared to WT strains, or bhd1Δ and ypt71Δ strains (Fig. 5b). ------- COMMENT: 12517e47bd2ece51 6 h+rN06tu68IEh3peN2uiGFj4YMw Strains lacking Ypt7 (ypt7Δ, bhd1Δ ypt7Δ, ypt71Δ ypt7Δ) displayed a significant growth defect in the low amino-acid condition (EMM plates supplemented with low concentration of amino acids) compared to WT strains, or bhd1Δ and ypt71Δ strains (Fig. 5b). ------- COMMENT: 12517e47bd2ece51 7 h+rN06tu68IEh3peN2uiGFj4YMw Strains lacking Ypt7 (ypt7Δ, bhd1Δ ypt7Δ, ypt71Δ ypt7Δ) displayed a significant growth defect in the low amino-acid condition (EMM plates supplemented with low concentration of amino acids) compared to WT strains, or bhd1Δ and ypt71Δ strains (Fig. 5b). ------- COMMENT: 12517e47bd2ece51 8 lxYGg+zyPtX00kXoqE43l62F4HY To determine whether these proteins are related to TORC1 signalling, we treated bhd1Δ, ypt71Δ, ypt7Δ and double deletion strains with 200 ng ml−1 of rapamycin and found that all of the strains grew better than WT cells (Fig. 5b). ------- COMMENT: 12517e47bd2ece51 9 lxYGg+zyPtX00kXoqE43l62F4HY To determine whether these proteins are related to TORC1 signalling, we treated bhd1Δ, ypt71Δ, ypt7Δ and double deletion strains with 200 ng ml−1 of rapamycin and found that all of the strains grew better than WT cells (Fig. 5b). ------- COMMENT: 12517e47bd2ece51 10 lxYGg+zyPtX00kXoqE43l62F4HY To determine whether these proteins are related to TORC1 signalling, we treated bhd1Δ, ypt71Δ, ypt7Δ and double deletion strains with 200 ng ml−1 of rapamycin and found that all of the strains grew better than WT cells (Fig. 5b). ------- COMMENT: 12517e47bd2ece51 11 4D4f6MrYDXff+SRsLM/Q4KOlt+o These data suggest that Bhd1 and Ypt71 (but not Ypt7) functionally interact and, in agreement with the mammalian cell data, negatively regulate TORC1 activity in response to amino-acid deprivation. ------- COMMENT: 12517e47bd2ece51 12 4D4f6MrYDXff+SRsLM/Q4KOlt+o These data suggest that Bhd1 and Ypt71 (but not Ypt7) functionally interact and, in agreement with the mammalian cell data, negatively regulate TORC1 activity in response to amino-acid deprivation. ------- COMMENT: 12517e47bd2ece51 13 LGfpHLn9h4uWmR3sx2qpmMIutWw figure 5b ------- COMMENT: 12517e47bd2ece51 14 LGfpHLn9h4uWmR3sx2qpmMIutWw figure 5b ------- COMMENT: 12517e47bd2ece51 15 LGfpHLn9h4uWmR3sx2qpmMIutWw figure 5b ------- COMMENT: 129db89392d735c1 8 ceyUArxdXYg+KLd8n/F/VO9ARRk Ras activity increases during the mating process and is maximum at the fusion site just before the fusion event. ------- COMMENT: 129db89392d735c1 10 90cqqbO05Db6H339GsB4ezcGtcM Active Ras1 co-localizes with the actin fusion focus during the process of cell-cell fusion ------- COMMENT: 129db89392d735c1 11 9Yk/phcYXtbM/WI3hcG3RZ9qOd8 ras1 mutant cells undergo precocious fusion resulting in cell lysis ------- COMMENT: 129db89392d735c1 12 9Yk/phcYXtbM/WI3hcG3RZ9qOd8 ras1 mutant cells undergo precocious fusion resulting in cell lysis ------- COMMENT: 129db89392d735c1 13 MJdE15URaO998m5FWFPckKp5lvc Active Ras1 is localized to cell poles during mitotic growth ------- COMMENT: 129db89392d735c1 14 zzYlKuNHNs2TBi2gCo/fX6SZU9o Ative Ras1 localizes to septa during mitotic growth ------- COMMENT: 129db89392d735c1 15 gAE62O7pLJIXpd/XvQ0p5SNaYvA fus1 deletion suppresses cell lysis of cells lacking Gap1 ------- COMMENT: 129db89392d735c1 16 NAGLreJWq219DS1v9y82zOcCoWY Ste6 co-localizes with the actin fusion focus during the process of cell-cell fusion ------- COMMENT: 129db89392d735c1 19 MJdE15URaO998m5FWFPckKp5lvc Active Ras1 is localized to cell poles during mitotic growth ------- COMMENT: 129db89392d735c1 20 MJdE15URaO998m5FWFPckKp5lvc Active Ras1 is localized to cell poles during mitotic growth ------- COMMENT: 129db89392d735c1 22 hVpfQNpB1GdGHD97BA7MEMWlvNY In the absence of efc25 Ras1 is not activated at the cell cortex ------- COMMENT: 129db89392d735c1 24 hVpfQNpB1GdGHD97BA7MEMWlvNY In the absence of efc25 Ras1 is not activated at the cell cortex ------- COMMENT: 129db89392d735c1 35 jlQGTGK2w+YIb9jey4nIevi/iPA In the absence of gap1 Ras activity increases and decorates the entire cortex of vegetative growing cells ------- COMMENT: 12c405f9c577461c 1 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 12c405f9c577461c 2 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 12c405f9c577461c 3 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 12c405f9c577461c 4 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 12c405f9c577461c 5 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 12c405f9c577461c 6 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 12c405f9c577461c 7 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 12c405f9c577461c 8 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 12c405f9c577461c 9 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 12c405f9c577461c 10 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 12c405f9c577461c 11 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 12c405f9c577461c 12 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 12c405f9c577461c 13 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 12c405f9c577461c 14 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 12c405f9c577461c 15 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 12c405f9c577461c 16 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 12c405f9c577461c 17 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 12c405f9c577461c 18 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 12c405f9c577461c 19 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 12c405f9c577461c 20 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 12c405f9c577461c 21 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 12c405f9c577461c 22 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 12c405f9c577461c 23 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 12c405f9c577461c 24 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 12c405f9c577461c 25 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 12c405f9c577461c 26 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 12c405f9c577461c 27 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 12c405f9c577461c 28 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 12c405f9c577461c 29 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 12c405f9c577461c 30 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 12c405f9c577461c 31 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 12c405f9c577461c 32 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 12c405f9c577461c 33 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 12c405f9c577461c 34 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 12c405f9c577461c 35 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 12c405f9c577461c 36 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 12c405f9c577461c 37 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 12c405f9c577461c 38 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 12c405f9c577461c 39 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 12c405f9c577461c 40 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 12c405f9c577461c 41 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 12c405f9c577461c 42 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 12c405f9c577461c 43 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 12c405f9c577461c 44 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 12c405f9c577461c 45 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 12c405f9c577461c 46 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 12c405f9c577461c 47 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 12c405f9c577461c 48 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 12c405f9c577461c 49 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 12c405f9c577461c 50 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 12c405f9c577461c 51 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 12c405f9c577461c 52 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 12c405f9c577461c 53 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 12c405f9c577461c 54 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 12c405f9c577461c 55 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 12c405f9c577461c 56 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 12c405f9c577461c 57 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 12c405f9c577461c 58 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 12c405f9c577461c 59 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 12c405f9c577461c 60 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 12c405f9c577461c 61 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 12c405f9c577461c 62 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 12c405f9c577461c 63 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 12c405f9c577461c 64 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 12c405f9c577461c 65 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 12c405f9c577461c 66 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 12c405f9c577461c 67 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 12c405f9c577461c 68 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 12c405f9c577461c 69 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 12c405f9c577461c 70 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 12c405f9c577461c 71 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 12c405f9c577461c 72 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 12c405f9c577461c 73 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 12c405f9c577461c 74 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 12c405f9c577461c 75 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: 12c405f9c577461c 76 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: 12c405f9c577461c 77 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: 12c405f9c577461c 78 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: 12c405f9c577461c 79 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: 12c405f9c577461c 80 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: 12c405f9c577461c 81 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: 12c405f9c577461c 82 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 12c405f9c577461c 83 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 12c405f9c577461c 84 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 12c405f9c577461c 85 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 12c405f9c577461c 86 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 12c405f9c577461c 87 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 12c405f9c577461c 88 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 12c405f9c577461c 89 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 12c405f9c577461c 90 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 12c405f9c577461c 91 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 12c405f9c577461c 92 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 12c405f9c577461c 93 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 12c405f9c577461c 94 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 12c405f9c577461c 95 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 12c405f9c577461c 96 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 12c405f9c577461c 97 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 12c405f9c577461c 98 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 12c405f9c577461c 99 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 12c405f9c577461c 100 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 12c405f9c577461c 101 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 12c405f9c577461c 102 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 12c405f9c577461c 103 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 12c405f9c577461c 104 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 12c405f9c577461c 105 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 12c405f9c577461c 106 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 12c405f9c577461c 107 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 12c405f9c577461c 108 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 12c405f9c577461c 109 KjATrBSC+WYh4Pv4fPJqIXKawb0 at the mat locus and subtelomeric repeats Set1-mediated H3K4me contributes to repression. ------- COMMENT: 12c405f9c577461c 110 KjATrBSC+WYh4Pv4fPJqIXKawb0 at the mat locus and subtelomeric repeats Set1-mediated H3K4me contributes to repression. ------- COMMENT: 12c405f9c577461c 112 wWlYXJUyEgQ1yu+X2XuRie3B12k Thus, H3K4me catalyzed by Set1C could compete with Mst1- mediated H3K4ac at Tf2s to maintain the integrity of Tf bodies. ------- COMMENT: 1305068212b0226d 1 +9SO/iDSmeShjXoS5GJxvzLEQIw data not shown, from text ------- COMMENT: 1305068212b0226d 7 +9SO/iDSmeShjXoS5GJxvzLEQIw data not shown, from text ------- COMMENT: 1305068212b0226d 8 +9SO/iDSmeShjXoS5GJxvzLEQIw data not shown, from text ------- COMMENT: 133b9a4cefba1a78 1 NX6v/toclXh9222PH8RUlVRwEAI data not shown ------- COMMENT: 133b9a4cefba1a78 2 NX6v/toclXh9222PH8RUlVRwEAI data not shown ------- COMMENT: 134ecf3bebd24e1a 1 T7L8A3vGKezVIUWh99YvVOXa6d0 (comment: major) ------- COMMENT: 134ecf3bebd24e1a 2 fuuBhSCZPimmMPcwF+TrNbVI0mY (comment: minor Hsp3106 has a lower in vitro glyoxalase III activity than Hsp3101 and Hsp3102) ------- COMMENT: 134ecf3bebd24e1a 3 T7L8A3vGKezVIUWh99YvVOXa6d0 (comment: major) ------- COMMENT: 1352d0cf7b415a6e 2 H65vdqSwPBWOPzoQb+uPbrRrXLE (comment: magneisum activated_by CHEBI:18420) ------- COMMENT: 13625faa87e9dab3 3 xjSs48HwZBag4nDCtgPgYfddc+I Fig. 3A, Fig. 3B Fig. S2, A and B IN TRANSIENT BURSTS & progressive increase in the number of Wee1 nodes as a function of cell size, ... 20X in Wee1 nodes in large cells versus small cells (Fig. 4 B). ------- COMMENT: 13625faa87e9dab3 6 vOAxq0CyYWIqyRPdkS9OUC/uRQ8 ------- COMMENT: 13625faa87e9dab3 7 IEcTKeke/r17xP39BcDKStIRyvs ------- COMMENT: 13625faa87e9dab3 8 115/+vLfR70/Xs51qvHak06/Fxg fig 3D ------- COMMENT: 13625faa87e9dab3 9 wyOwbN+QMPKreFi/KUJoe2EKHKo Fig. S2, F and G ------- COMMENT: 13625faa87e9dab3 10 zzsZ5+DvfRxE8lryZvqo8HgyGaw figure 2D (commment: Epistatic to cdr2delta) ------- COMMENT: 13625faa87e9dab3 11 3BZbvoQI/4F63kSGrIHs82LIdhk Fig 2C (comment: protein localizes to cytoplasm, nucleus, and spindle-pole body) ------- COMMENT: 13625faa87e9dab3 16 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: 13625faa87e9dab3 17 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: 13625faa87e9dab3 18 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 13625faa87e9dab3 19 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: 13625faa87e9dab3 20 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: 13625faa87e9dab3 21 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 13625faa87e9dab3 22 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: 13625faa87e9dab3 23 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: 13625faa87e9dab3 25 Krt8IEN2thpV+MLPwLEVMzonARU Fig. 2B ------- COMMENT: 13625faa87e9dab3 26 MRv4U0wptZfY8tXQDHMhzMNic0c Fig. 2B ------- COMMENT: 13625faa87e9dab3 27 MRv4U0wptZfY8tXQDHMhzMNic0c Fig. 2B ------- COMMENT: 13625faa87e9dab3 28 MRv4U0wptZfY8tXQDHMhzMNic0c Fig. 2B ------- COMMENT: 13625faa87e9dab3 29 zzsZ5+DvfRxE8lryZvqo8HgyGaw Fig. 2D (comment: Epistatic to cdr2delta) ------- COMMENT: 13b6f989def8efa6 70 vp14BNoSA0zL3HnqjLkxVtI/Oiw ------- COMMENT: 13bf17b19bdebfdb 1 UFPL+Hx5denxfx0rYYNW6dihtEg The cis4-1 mutant cells grew well in rich YPD medium, however, in the presence of 0.15 M MgCl2, the cis4-1 cells failed to grow, whereas wild-type cells grew well (Figure 1A). ------- COMMENT: 13bf17b19bdebfdb 2 OAbueFXwk4N9lCVGduDN/C+/DCU Notably, overex- pression of the ecm33+ gene partially suppressed the MgCl2 sensitivity of cis4-1 mutant, and overexpression of the aah3+ and gaz2+ genes more strongly suppressed the MgCl2 sensitivity of the cis4-1 mutant (Figure 1A). ------- COMMENT: 13bf17b19bdebfdb 3 OAbueFXwk4N9lCVGduDN/C+/DCU Notably, overex- pression of the ecm33+ gene partially suppressed the MgCl2 sensitivity of cis4-1 mutant, and overexpression of the aah3+ and gaz2+ genes more strongly suppressed the MgCl2 sensitivity of the cis4-1 mutant (Figure 1A). ------- COMMENT: 13bf17b19bdebfdb 4 OAbueFXwk4N9lCVGduDN/C+/DCU Notably, overex- pression of the ecm33+ gene partially suppressed the MgCl2 sensitivity of cis4-1 mutant, and overexpression of the aah3+ and gaz2+ genes more strongly suppressed the MgCl2 sensitivity of the cis4-1 mutant (Figure 1A). ------- COMMENT: 13bf17b19bdebfdb 5 zSUR/cbjHLXWq+OXtvVNJq8Ru1A Likewise, these three genes complemented the FK506-sensitive phenotype of the cis4-1 mutant (Figure 1A). ------- COMMENT: 13bf17b19bdebfdb 6 zSUR/cbjHLXWq+OXtvVNJq8Ru1A Likewise, these three genes complemented the FK506-sensitive phenotype of the cis4-1 mutant (Figure 1A). ------- COMMENT: 13bf17b19bdebfdb 7 zSUR/cbjHLXWq+OXtvVNJq8Ru1A Likewise, these three genes complemented the FK506-sensitive phenotype of the cis4-1 mutant (Figure 1A). ------- COMMENT: 13bf17b19bdebfdb 8 zSUR/cbjHLXWq+OXtvVNJq8Ru1A Likewise, these three genes complemented the FK506-sensitive phenotype of the cis4-1 mutant (Figure 1A). ------- COMMENT: 13bf17b19bdebfdb 9 G6eW9Ghiz1Sq7pwpx621ySEns7c We constructed a null mutation in the ecm33+ and gaz2+ genes, respectively (see Materials and Methods) and found that the gaz2 deletion mutant was also viable (Figure 2A, upper panel), indicating that Gaz2 is not essential for cell viability. ------- COMMENT: 13bf17b19bdebfdb 10 G6eW9Ghiz1Sq7pwpx621ySEns7c We constructed a null mutation in the ecm33+ and gaz2+ genes, respectively (see Materials and Methods) and found that the gaz2 deletion mutant was also viable (Figure 2A, upper panel), indicating that Gaz2 is not essential for cell viability. ------- COMMENT: 13bf17b19bdebfdb 11 lpq/bp1uc8Qhf3FWWrXV+GmfMNo (Figure 2) ------- COMMENT: 13bf17b19bdebfdb 12 lpq/bp1uc8Qhf3FWWrXV+GmfMNo (Figure 2) ------- COMMENT: 13bf17b19bdebfdb 13 lpq/bp1uc8Qhf3FWWrXV+GmfMNo (Figure 2) ------- COMMENT: 13bf17b19bdebfdb 14 lpq/bp1uc8Qhf3FWWrXV+GmfMNo (Figure 2) ------- COMMENT: 13bf17b19bdebfdb 15 lpq/bp1uc8Qhf3FWWrXV+GmfMNo (Figure 2) ------- COMMENT: 13bf17b19bdebfdb 16 YgyBxGt0ZaWipPLb3xj7yYfeY5g (Figure 3B) ------- COMMENT: 13bf17b19bdebfdb 17 YgyBxGt0ZaWipPLb3xj7yYfeY5g (Figure 3B) ------- COMMENT: 13bf17b19bdebfdb 18 YgyBxGt0ZaWipPLb3xj7yYfeY5g (Figure 3B) ------- COMMENT: 13bf17b19bdebfdb 19 YgyBxGt0ZaWipPLb3xj7yYfeY5g (Figure 3B) ------- COMMENT: 13bf17b19bdebfdb 20 YgyBxGt0ZaWipPLb3xj7yYfeY5g (Figure 3B) ------- COMMENT: 13bf17b19bdebfdb 21 YgyBxGt0ZaWipPLb3xj7yYfeY5g (Figure 3B) ------- COMMENT: 13bf17b19bdebfdb 22 YgyBxGt0ZaWipPLb3xj7yYfeY5g (Figure 3B) ------- COMMENT: 13bf17b19bdebfdb 23 YgyBxGt0ZaWipPLb3xj7yYfeY5g (Figure 3B) ------- COMMENT: 13bf17b19bdebfdb 24 YgyBxGt0ZaWipPLb3xj7yYfeY5g (Figure 3B) ------- COMMENT: 13bf17b19bdebfdb 25 YgyBxGt0ZaWipPLb3xj7yYfeY5g (Figure 3B) ------- COMMENT: 13bf17b19bdebfdb 26 YgyBxGt0ZaWipPLb3xj7yYfeY5g (Figure 3B) ------- COMMENT: 13bf17b19bdebfdb 27 gM37fp9BP/2r1xj+81IhVAWGXnw (Figure 3C) The immunoblot analysis detected an appreciable amount of Ecm33 fragments A, C, F, G, and I ------- COMMENT: 13bf17b19bdebfdb 28 gM37fp9BP/2r1xj+81IhVAWGXnw (Figure 3C) The immunoblot analysis detected an appreciable amount of Ecm33 fragments A, C, F, G, and I ------- COMMENT: 13bf17b19bdebfdb 29 gM37fp9BP/2r1xj+81IhVAWGXnw (Figure 3C) The immunoblot analysis detected an appreciable amount of Ecm33 fragments A, C, F, G, and I ------- COMMENT: 13bf17b19bdebfdb 30 gM37fp9BP/2r1xj+81IhVAWGXnw (Figure 3C) The immunoblot analysis detected an appreciable amount of Ecm33 fragments A, C, F, G, and I ------- COMMENT: 13bf17b19bdebfdb 31 gM37fp9BP/2r1xj+81IhVAWGXnw (Figure 3C) The immunoblot analysis detected an appreciable amount of Ecm33 fragments A, C, F, G, and I ------- COMMENT: 13bf17b19bdebfdb 32 pFtwd/Pq378j2CXduvkh13Oi+oc (Figure 3C) but failed to detect fragment B, D, E, H, J or K. ------- COMMENT: 13bf17b19bdebfdb 33 pFtwd/Pq378j2CXduvkh13Oi+oc (Figure 3C) but failed to detect fragment B, D, E, H, J or K. ------- COMMENT: 13bf17b19bdebfdb 34 pFtwd/Pq378j2CXduvkh13Oi+oc (Figure 3C) but failed to detect fragment B, D, E, H, J or K. ------- COMMENT: 13bf17b19bdebfdb 35 pFtwd/Pq378j2CXduvkh13Oi+oc (Figure 3C) but failed to detect fragment B, D, E, H, J or K. ------- COMMENT: 13bf17b19bdebfdb 36 pFtwd/Pq378j2CXduvkh13Oi+oc (Figure 3C) but failed to detect fragment B, D, E, H, J or K. ------- COMMENT: 13bf17b19bdebfdb 37 pFtwd/Pq378j2CXduvkh13Oi+oc (Figure 3C) but failed to detect fragment B, D, E, H, J or K. ------- COMMENT: 13bf17b19bdebfdb 38 4su3RfOJYBbcKhnPsJivJ5JVN2Y Ecm33 localized to the cell surface or the medial regions. (figure 4) ------- COMMENT: 13bf17b19bdebfdb 39 Rav3AMS6pCGTtTqcrBdvO26UUkA As expected, GFP-Ecm33 primarily localized to the ER and to the cell surface in the its8-1 mutant cells (Figure 4D, arrows), suggesting that the impairment of GPI anchor synthesis caused the defective attachment of GPI-anchor to the Ecm33 protein thereby resulting in the abnormal GFP-Ecm33 localization in the ER. ------- COMMENT: 13bf17b19bdebfdb 40 AZbqkBO3F+W7e26OfxrFXOdQOqQ The results showed that the addition of Zn2+ to the medium significantly rescued the high temperature-sensitive and FK506-sensitive phenotypes of the its8-1 mutant (Figure 5A) ------- COMMENT: 13bf17b19bdebfdb 41 AZbqkBO3F+W7e26OfxrFXOdQOqQ The results showed that the addition of Zn2+ to the medium significantly rescued the high temperature-sensitive and FK506-sensitive phenotypes of the its8-1 mutant (Figure 5A) ------- COMMENT: 13bf17b19bdebfdb 42 /9GBzYlagbFa/Ly98z5MkembIq0 On the effect of temperature, in the its8-1Dcis4 double mutants, these cells exhibited more marked temperature sensitivity than that of the its8-1 single mutants (Figure 5B), suggesting that there is a genetic interaction between Its8 and Cis4. ------- COMMENT: 13bf17b19bdebfdb 43 /9GBzYlagbFa/Ly98z5MkembIq0 On the effect of temperature, in the its8-1Dcis4 double mutants, these cells exhibited more marked temperature sensitivity than that of the its8-1 single mutants (Figure 5B), suggesting that there is a genetic interaction between Its8 and Cis4. ------- COMMENT: 13bf17b19bdebfdb 44 V7uHBuK2ctrB24vWaX2CUZcRdrk (comment: ****decreased to cell surface, mislocalized to cytoplasm*****) In Dapm1 cells, in contrast, GFP-Ecm33 primarily localized as dot-like structures that were observed in the cytoplasm (Figure 6A, arrows) as well as at the cell surface and the division site (Figure 6A, arrowheads). ------- COMMENT: 13bf17b19bdebfdb 45 XhnMJeOou3iWrJYJ3A1bUCxjZ5E Thus, GFP-Ecm33 localized at Golgi/endosome structures in addition to the cell surface and the division site in these mutants, suggesting that GPI-anchored proteins were not correctly transported and were retained at the Golgi/endosome structures in these membrane trafficking mutants ------- COMMENT: 13bf17b19bdebfdb 46 XhnMJeOou3iWrJYJ3A1bUCxjZ5E Thus, GFP-Ecm33 localized at Golgi/endosome structures in addition to the cell surface and the division site in these mutants, suggesting that GPI-anchored proteins were not correctly transported and were retained at the Golgi/endosome structures in these membrane trafficking mutants ------- COMMENT: 13bf17b19bdebfdb 47 XhnMJeOou3iWrJYJ3A1bUCxjZ5E Thus, GFP-Ecm33 localized at Golgi/endosome structures in addition to the cell surface and the division site in these mutants, suggesting that GPI-anchored proteins were not correctly transported and were retained at the Golgi/endosome structures in these membrane trafficking mutants ------- COMMENT: 13bf17b19bdebfdb 48 XhnMJeOou3iWrJYJ3A1bUCxjZ5E Thus, GFP-Ecm33 localized at Golgi/endosome structures in addition to the cell surface and the division site in these mutants, suggesting that GPI-anchored proteins were not correctly transported and were retained at the Golgi/endosome structures in these membrane trafficking mutants ------- COMMENT: 13bf17b19bdebfdb 49 XhnMJeOou3iWrJYJ3A1bUCxjZ5E Thus, GFP-Ecm33 localized at Golgi/endosome structures in addition to the cell surface and the division site in these mutants, suggesting that GPI-anchored proteins were not correctly transported and were retained at the Golgi/endosome structures in these membrane trafficking mutants ------- COMMENT: 13bf17b19bdebfdb 50 XhnMJeOou3iWrJYJ3A1bUCxjZ5E Thus, GFP-Ecm33 localized at Golgi/endosome structures in addition to the cell surface and the division site in these mutants, suggesting that GPI-anchored proteins were not correctly transported and were retained at the Golgi/endosome structures in these membrane trafficking mutants ------- COMMENT: 13bf17b19bdebfdb 51 Q3N9IKuB/Lrf9xi0j5XQ7laCvFk Similarly, in the wild-type cells, GFP-Gaz2 also clearly localized at the cell surface and medial regions (Figure 6C), while in Dapm1 cells, GFP-Gaz2 localized as intracellular dot-like structures (Figure 6C). ------- COMMENT: 13bf17b19bdebfdb 52 Q3N9IKuB/Lrf9xi0j5XQ7laCvFk Similarly, in the wild-type cells, GFP-Gaz2 also clearly localized at the cell surface and medial regions (Figure 6C), while in Dapm1 cells, GFP-Gaz2 localized as intracellular dot-like structures (Figure 6C). ------- COMMENT: 13bf17b19bdebfdb 53 /9GBzYlagbFa/Ly98z5MkembIq0 On the effect of temperature, in the its8-1Dcis4 double mutants, these cells exhibited more marked temperature sensitivity than that of the its8-1 single mutants (Figure 5B), suggesting that there is a genetic interaction between Its8 and Cis4. ------- COMMENT: 13c6c57e6425bca9 13 3aCeJ+M6rzJumBNwl+gXNaWSkx4 (comment: population is viable, but very small cells look lysed) ------- COMMENT: 13c6c57e6425bca9 15 htVfjxL0SMrtk+koAbMi124IFFg (comment: population is viable but sick, and the elongated multiseptate cells are probably dead) ------- COMMENT: 13c6c57e6425bca9 21 yhZ1c5/8SMUIoLwRQ1DmLxiURbw (comment: population is viable but sick; can't tell which individual cells are viable) ------- COMMENT: 13c6c57e6425bca9 27 NjBpXoSfz4zg3c/wreyPYPEZD5s (comment: population grows well, but very small cells look lysed) ------- COMMENT: 13c6c57e6425bca9 32 yhZ1c5/8SMUIoLwRQ1DmLxiURbw (comment: population is viable but sick; can't tell which individual cells are viable) ------- COMMENT: 13c6c57e6425bca9 33 cG3pllUVNPKUQwnq8TtWBmVdCBU (comment: population is viable but sick; can't tell which individual cells are viable, but very small cells look lysed) ------- COMMENT: 13c8454e4e123cb3 115 0n5gEA08hNcCZnnh9vVQR8nWJIs ------- COMMENT: 13e26365fef6c9cf 1 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 13e26365fef6c9cf 2 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 3 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 4 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 5 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 6 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 7 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 8 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 9 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 10 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 11 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 12 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 13e26365fef6c9cf 13 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 13e26365fef6c9cf 14 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 13e26365fef6c9cf 15 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 13e26365fef6c9cf 16 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 13e26365fef6c9cf 17 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 13e26365fef6c9cf 18 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 13e26365fef6c9cf 19 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 13e26365fef6c9cf 20 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 13e26365fef6c9cf 21 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 13e26365fef6c9cf 22 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 13e26365fef6c9cf 23 lt2pkx5XPTUurrYIIsqW8bTRKF8 Fig. 7C ------- COMMENT: 13e26365fef6c9cf 26 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 13e26365fef6c9cf 27 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 13e26365fef6c9cf 29 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 13e26365fef6c9cf 30 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 13e26365fef6c9cf 31 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 13e26365fef6c9cf 32 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 13e26365fef6c9cf 33 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 13e26365fef6c9cf 34 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 13e26365fef6c9cf 35 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 13e26365fef6c9cf 36 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 13e26365fef6c9cf 37 r1qmptYtrKtD+ODO9QKWH2C1ryE Fig. 4D and E ------- COMMENT: 13e26365fef6c9cf 38 r1qmptYtrKtD+ODO9QKWH2C1ryE Fig. 4D and E ------- COMMENT: 13e26365fef6c9cf 39 r1qmptYtrKtD+ODO9QKWH2C1ryE Fig. 4D and E ------- COMMENT: 13e26365fef6c9cf 40 r1qmptYtrKtD+ODO9QKWH2C1ryE Fig. 4D and E ------- COMMENT: 13e26365fef6c9cf 41 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 13e26365fef6c9cf 42 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 13e26365fef6c9cf 43 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 13e26365fef6c9cf 44 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 13e26365fef6c9cf 59 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 13e26365fef6c9cf 60 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 61 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 62 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 63 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 75 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 76 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 77 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 78 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 79 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 80 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 81 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 82 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 83 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 84 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 85 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 86 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 87 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 88 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 89 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 90 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 91 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 92 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 93 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 94 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 95 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 96 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 97 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 98 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 99 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 100 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 101 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 102 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 103 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 104 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 105 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 106 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 107 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 108 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 109 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 110 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 111 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 112 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 113 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 114 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 115 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 116 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 117 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 118 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 119 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 120 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 121 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 122 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 123 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 124 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 125 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 126 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 127 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 128 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 129 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 130 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 131 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 132 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 133 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 134 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 135 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 136 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 137 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 138 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 139 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 140 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 141 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 142 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 143 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 144 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 145 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 146 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 147 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 148 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 149 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 150 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 151 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 152 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 153 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 154 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 155 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 156 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 157 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 158 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 159 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 160 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 161 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 162 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 13e26365fef6c9cf 163 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 13e26365fef6c9cf 164 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 13e26365fef6c9cf 165 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 13e26365fef6c9cf 166 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 13e26365fef6c9cf 167 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 13e26365fef6c9cf 168 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 13e26365fef6c9cf 169 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 13e26365fef6c9cf 170 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 13e26365fef6c9cf 171 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 13e26365fef6c9cf 172 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 13e26365fef6c9cf 173 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 13e26365fef6c9cf 174 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 13e26365fef6c9cf 175 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 13e26365fef6c9cf 176 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 13f535eec84725d1 18 zA1zOyz5cAha0mo5xU71keB7SRk ------- COMMENT: 13f535eec84725d1 31 or/gc0SLqxXx7cXaa/cUUC6T+FI ------- COMMENT: 140fc39c1d9e9588 1 6xiL+1wBMiMjVvtRPsjQfOV2gi8 The dosage increase of Shp2 promotes Pi export activity in an Xpr1-independent manner. ------- COMMENT: 140fc39c1d9e9588 2 e5W4BLp3a0hG5tT5UacXeqbWPO4 pqr1∆xpr1∆shp2∆ exibits severe defect in Pi export. ------- COMMENT: 140fc39c1d9e9588 3 QW0aa9vU86jbfZkZpbl5j/EqnTk pqr1∆xpr1∆shp2∆ exibits severe growth defect at 100 mM Pi, where pqr1∆xpr1∆ can grow. ------- COMMENT: 1445a23bf68725db 1 so4SE2pksambUPfmgSGL9nibVoI Also affects sister chromatid segregation ------- COMMENT: 1450287ab46a1564 1 pzq8lsX0eiiVARJZ12xdlV+/kRg Fig 1 (comment: 16.5 +/- 0.78) ------- COMMENT: 1450287ab46a1564 2 Jbow++vJPLs+hcIufzBgGZUdaPE Fig 1 (comment: IS THIS SMALL OR STUBBY? 11.6 +/- 0.45) ------- COMMENT: 1450287ab46a1564 3 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 1450287ab46a1564 4 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 1450287ab46a1564 5 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 1450287ab46a1564 6 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 1450287ab46a1564 7 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 1450287ab46a1564 8 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: 1450287ab46a1564 9 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: 1450287ab46a1564 10 +ELLjwUfK5aTh8y7xc8m451gJk4 Fig 1 C ------- COMMENT: 1450287ab46a1564 11 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: 1450287ab46a1564 12 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: 1450287ab46a1564 13 GSDoKVHrCsiUO6zYP0Vg8a8VAAQ fig 1D ------- COMMENT: 1450287ab46a1564 14 GSDoKVHrCsiUO6zYP0Vg8a8VAAQ fig 1D ------- COMMENT: 1450287ab46a1564 15 GSDoKVHrCsiUO6zYP0Vg8a8VAAQ fig 1D ------- COMMENT: 1450287ab46a1564 16 GSDoKVHrCsiUO6zYP0Vg8a8VAAQ fig 1D ------- COMMENT: 1450287ab46a1564 17 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: 1450287ab46a1564 18 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: 1450287ab46a1564 19 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: 1450287ab46a1564 20 wj2VSE893AMBlEWR8+9Ey55om+8 Fig 2B ------- COMMENT: 1450287ab46a1564 21 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: 1450287ab46a1564 22 wj2VSE893AMBlEWR8+9Ey55om+8 Fig 2B ------- COMMENT: 1450287ab46a1564 23 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: 1450287ab46a1564 24 wj2VSE893AMBlEWR8+9Ey55om+8 Fig 2B ------- COMMENT: 1450287ab46a1564 25 wj2VSE893AMBlEWR8+9Ey55om+8 Fig 2B ------- COMMENT: 1450287ab46a1564 26 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: 1450287ab46a1564 27 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: 1450287ab46a1564 28 6pSAHAPl4EEMTDqkfHAb9E42zmg Fig 2D ------- COMMENT: 1450287ab46a1564 29 6pSAHAPl4EEMTDqkfHAb9E42zmg Fig 2D ------- COMMENT: 1450287ab46a1564 30 8zdno63XFs3BFsVP161LRtfCMNM Figure 3D ------- COMMENT: 1450287ab46a1564 33 wFoDbeBDCUTevS781Vedd+eKOB4 figure 4A ------- COMMENT: 1450287ab46a1564 34 7wzkPu+Q00RMOxsquqPJJn/p0gc figure 4A (comment: IS THIS NORMAL OR EVEN HIGHER THAN WT?) ------- COMMENT: 1450287ab46a1564 35 QI3ia37hw0Quhwpu4r8X5USkScA figure 4A (comment: IS THIS NORMAL OR EVEN HIGHER THAN WT? this is higher than wis1DD-cpc2delet so must be increased) ------- COMMENT: 1450287ab46a1564 36 8zdno63XFs3BFsVP161LRtfCMNM Figure 3D ------- COMMENT: 1450287ab46a1564 37 8zdno63XFs3BFsVP161LRtfCMNM Figure 3D ------- COMMENT: 1450287ab46a1564 38 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: 1450287ab46a1564 39 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: 1450287ab46a1564 40 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: 1450287ab46a1564 41 bBKa+cOSgxLimLqWZYMQioRzSdA Figure 4c ------- COMMENT: 1450287ab46a1564 42 bBKa+cOSgxLimLqWZYMQioRzSdA Figure 4c ------- COMMENT: 1450287ab46a1564 43 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: 1450287ab46a1564 44 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: 1450287ab46a1564 45 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: 1450287ab46a1564 46 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: 1450287ab46a1564 47 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: 1450287ab46a1564 48 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: 1450287ab46a1564 49 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: 1450287ab46a1564 50 o/5kly/nkbqChQ0Nr/1PKVwtSfQ figure 5D ------- COMMENT: 1450287ab46a1564 51 o/5kly/nkbqChQ0Nr/1PKVwtSfQ figure 5D ------- COMMENT: 1450287ab46a1564 52 yBkIVbEbeQTDY5BpoRoJKQduY58 figure 6a ------- COMMENT: 1450287ab46a1564 53 yBkIVbEbeQTDY5BpoRoJKQduY58 figure 6a ------- COMMENT: 1450287ab46a1564 54 yBkIVbEbeQTDY5BpoRoJKQduY58 figure 6a ------- COMMENT: 1450287ab46a1564 55 yBkIVbEbeQTDY5BpoRoJKQduY58 figure 6a ------- COMMENT: 1450287ab46a1564 56 yBkIVbEbeQTDY5BpoRoJKQduY58 figure 6a ------- COMMENT: 1450287ab46a1564 57 yBkIVbEbeQTDY5BpoRoJKQduY58 figure 6a ------- COMMENT: 1450287ab46a1564 58 yBkIVbEbeQTDY5BpoRoJKQduY58 figure 6a ------- COMMENT: 1450287ab46a1564 59 yBkIVbEbeQTDY5BpoRoJKQduY58 figure 6a ------- COMMENT: 1450287ab46a1564 60 yBkIVbEbeQTDY5BpoRoJKQduY58 figure 6a ------- COMMENT: 1450287ab46a1564 61 yBkIVbEbeQTDY5BpoRoJKQduY58 figure 6a ------- COMMENT: 1450287ab46a1564 62 96P2btLLJxvZMStFkPVzVR3pRtM figure 6c,d ------- COMMENT: 1450287ab46a1564 63 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 1450287ab46a1564 65 uP2b450kx3y/u6z3D7bDUa+eEuM fig 8 ------- COMMENT: 1450287ab46a1564 66 uP2b450kx3y/u6z3D7bDUa+eEuM fig 8 ------- COMMENT: 1450287ab46a1564 67 E7dxSeRImNv1w9YVo4ldky0E//U fig 9A ------- COMMENT: 1450287ab46a1564 68 nsjl/AexxpV5l54QnCPuNaqTUqc Figure 9B ------- COMMENT: 1450287ab46a1564 69 uqXsb7MQNxe+VxeldTsAIEIvtLk Figure 9 ------- COMMENT: 1450287ab46a1564 70 pqMuRwz8vEfyBkvL9Pfc2T6yDkk Figure 9 ------- COMMENT: 1456d39b083c07d3 1 DepE3SoJvDImV12yU4fJ+L5iL8U (comment: ***ABOLISHED*****) Whereas ago1Δ alleviated silencing of a ura4+ reporter inserted at an outer centromeric repeat region (otr1R::ura4+), simultaneous deletions of mlo3 and ago1 restored centromeric silencing (Fig. 1a). T ------- COMMENT: 1456d39b083c07d3 2 5IFPXoKn5jBrex2asT89fxHhVzk Whereas ago1Δ alleviated silencing of a ura4+ reporter inserted at an outer centromeric repeat region (otr1R::ura4+), simultaneous deletions of mlo3 and ago1 restored centromeric silencing (Fig. 1a). T ------- COMMENT: 1456d39b083c07d3 3 aLFCEvScLzSHCbSgmO38snH7PcU (Fig. 1a). ------- COMMENT: 1456d39b083c07d3 4 dNQjW+k7rsAny2y74kKImcmiTOA (Supplementary Fig. 1) ------- COMMENT: 1456d39b083c07d3 5 dNQjW+k7rsAny2y74kKImcmiTOA (Supplementary Fig. 1) ------- COMMENT: 1456d39b083c07d3 6 Zlm7+a9L2E0IA3VBcp0bCTLunUw More importantly, mlo3Δ restored H3K9me and Swi6 localization at otr1R::ura4+ and endogenous centromeric repeats in ago1Δ mutant (Fig. 1 c–d) ------- COMMENT: 1456d39b083c07d3 7 Zlm7+a9L2E0IA3VBcp0bCTLunUw More importantly, mlo3Δ restored H3K9me and Swi6 localization at otr1R::ura4+ and endogenous centromeric repeats in ago1Δ mutant (Fig. 1 c–d) ------- COMMENT: 1456d39b083c07d3 8 YnBywaK15ZdklgS8XyzPWxP+kps mlo3Δ also restored silencing and heterochromatin formation at centromeres in dcr1Δ mutant (Supplementary Fig. 2a). ------- COMMENT: 1456d39b083c07d3 9 5/hCwAO6HP++p7LWzNBgBCzsADg As expected, cells carrying ago1Δ or dcr1Δ showed severe sensitivity to TBZ, (figure 1e) ------- COMMENT: 1456d39b083c07d3 10 5/hCwAO6HP++p7LWzNBgBCzsADg As expected, cells carrying ago1Δ or dcr1Δ showed severe sensitivity to TBZ, (figure 1e) ------- COMMENT: 1456d39b083c07d3 11 5/hCwAO6HP++p7LWzNBgBCzsADg As expected, cells carrying ago1Δ or dcr1Δ showed severe sensitivity to TBZ, (figure 1e) ------- COMMENT: 1456d39b083c07d3 12 ocHQQqcCNlaaUP53hvW+b0FfOtQ As expected for a factor involved in mRNP formation26,30, Mlo3 interacted with a euchromatic gene (fbp1) transcript (Fig. 1f). ------- COMMENT: 1456d39b083c07d3 13 J0tczA/jH2PVDZRWaxSYHur45wA Importantly, Mlo3 also interacted with dh transcript (Fig. 1f), consistent with results of ChIP analyses showing Mlo3 enrichment at transcribing centromeric repeats30.As expected for a factor involved in mRNP formation26,30, Mlo3 interacted with a euchromatic gene (fbp1) transcript (Fig. 1f). ------- COMMENT: 1456d39b083c07d3 14 231fpAyii+tSnFh6FckRA6a57uc Mutant cells lacking SHREC subunit Clr3 show marked increase in RNAPII occupancy at centromeric repeats10,11,31 ------- COMMENT: 1456d39b083c07d3 15 231fpAyii+tSnFh6FckRA6a57uc Mutant cells lacking SHREC subunit Clr3 show marked increase in RNAPII occupancy at centromeric repeats10,11,31 ------- COMMENT: 1456d39b083c07d3 16 3DUjWywd/2K4VHqgwd5j6Bk9x6E Deletion of tfs1, which led to 6- azauracil (6-AU) sensitivity (Fig. 2a) ------- COMMENT: 1456d39b083c07d3 17 A2LWajdlu8kvJ5oiQh2J0JFCRDE and changes in the distribution of RNAPII at body of genes (Supplementary Fig. 3c) ------- COMMENT: 1456d39b083c07d3 19 WS6MxRvo5KwW3iJDDJhN2NSVD5U resulted in variegated suppression of silencing defects in ago1Δ and dcr1Δ mutants (Fig. 2b and Supplementary Fig. 2b) ------- COMMENT: 1456d39b083c07d3 20 i6Kk4V04WzyD1mi1VhHHWcBKFl4 trs1delta failed to restore H3K9me at otr1R::ura4+ in clr3Δ ago1Δ cells (Fig. 3a). ------- COMMENT: 1456d39b083c07d3 21 i6Kk4V04WzyD1mi1VhHHWcBKFl4 trs1delta failed to restore H3K9me at otr1R::ura4+ in clr3Δ ago1Δ cells (Fig. 3a). ------- COMMENT: 1456d39b083c07d3 22 m4ASl34OXG6rghyYB+BKDZBb/FU In contrast, mlo3Δ resulted in considerable restoration of H3K9me at centromeres in clr3Δ ago1Δ cells (Fig. 3b). ------- COMMENT: 1456d39b083c07d3 23 wUaLZnkL8dxQ+JUrmC2vxBBrNvE Remarkably, loss of Cid14 subunit of TRAMP affects RNAi-independent heterochromatin formation in a manner similar to mlo3Δ. Loss of Cid14 restored H3K9me at otr1R::ura4+ and centromeric repeats in ago1Δ mutant (Fig. 4a–b). ------- COMMENT: 1456d39b083c07d3 24 30HkcVSWCg/VWMPUhHXG+9Y8bjI Moreover, cid14Δ suppressed the silencing defect caused by ago1Δ, as indicated by reduction in the levels of dg/dh transcript in cid14Δ ago1Δ as compared to ago1Δ (Fig. 4c). ------- COMMENT: 1456d39b083c07d3 25 Zlm7+a9L2E0IA3VBcp0bCTLunUw More importantly, mlo3Δ restored H3K9me and Swi6 localization at otr1R::ura4+ and endogenous centromeric repeats in ago1Δ mutant (Fig. 1 c–d) ------- COMMENT: 1456d39b083c07d3 26 Rrd78sIu+4DnakRvPKchGsz5L8U combining rrp6Δ with ago1Δ largely abolished H3K9me levels at otr1R::ura4+ and dg repeats (Fig. 5c). ------- COMMENT: 1456d39b083c07d3 27 TMxxA+ZB0BsAQ7Cv5Hh1l/NQYUA However, we found that simultaneous deletion of mlo3 and ago1 restored Rik1 enrichment at cenH (Fig. 6b). ------- COMMENT: 149d8ab5ac0f3ce8 40 ULInyfbYipHW3SkStb61YuA1L+4 (comment: same sensitivity as rhp54delta alone) ------- COMMENT: 149d8ab5ac0f3ce8 41 ULInyfbYipHW3SkStb61YuA1L+4 (comment: same sensitivity as rhp54delta alone) ------- COMMENT: 149d8ab5ac0f3ce8 42 uyXrSbaY8iIncD02YWRQ6f91N6k ------- COMMENT: 149d8ab5ac0f3ce8 43 uyXrSbaY8iIncD02YWRQ6f91N6k ------- COMMENT: 14d6b12f621eebfe 20 Z9HpRh6rE4CiHxIx/qUvOo3qupc (comment: temperature permissive for cdc8-27) ------- COMMENT: 14e1a7106407e8d4 2 U/ENf0bxvozcfBmnqXhA8JIjESU (comment: No queuosine-mediated reduction of translational errors at GGC (Gly) and UGC (Tyr) codons) ------- COMMENT: 14e1a7106407e8d4 3 mq8Dkhcgzh+9u8Sta+0p11OjqtM Reduced translation of transcripts with a mitochondrial function that is mediated by queuosine-modified tRNAs is abrogated in pmt1∆. ------- COMMENT: 14e1a7106407e8d4 4 O0RehoDo/h5MERO54UT2G6Pke8Y ... Q-modification in tRNAs is to improve translation ofC-ending codons relative to U-ending codons in S. pombe. ------- COMMENT: 14e1a7106407e8d4 6 mq8Dkhcgzh+9u8Sta+0p11OjqtM Reduced translation of transcripts with a mitochondrial function that is mediated by queuosine-modified tRNAs is abrogated in pmt1∆. ------- COMMENT: 1533fbdc3a541b0f 76 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment: same as either single mutant) ------- COMMENT: 1533fbdc3a541b0f 77 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment: same as either single mutant) ------- COMMENT: 1533fbdc3a541b0f 94 GClfETmhVHLg/BerzCuT7d1KL74 (comment: same as rad3delta alone) ------- COMMENT: 1533fbdc3a541b0f 95 GClfETmhVHLg/BerzCuT7d1KL74 (comment: same as rad3delta alone) ------- COMMENT: 1533fbdc3a541b0f 96 GClfETmhVHLg/BerzCuT7d1KL74 (comment: same as rad3delta alone) ------- COMMENT: 1533fbdc3a541b0f 97 GClfETmhVHLg/BerzCuT7d1KL74 (comment: same as rad3delta alone) ------- COMMENT: 1533fbdc3a541b0f 98 zUKmKhZc1WWjM/4LCry/+ZYpP+I (comment: same as rad26delta alone) ------- COMMENT: 1534156512e84afb 21 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 1534156512e84afb 22 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 153ada5377adf0b4 1 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 153ada5377adf0b4 2 PiJDmTtrtBpyNTS9yWwdTCAC+Xo Figure 1B/C ------- COMMENT: 153ada5377adf0b4 3 Y83rviqES89Rlz41WVFfzQ6mXWM Figure (comment: we modelled this increased duration of cohesion in mitotic anaphase, and the ectopic rec8 expression is now part of the genotype,does that sound OK?) ------- COMMENT: 153ada5377adf0b4 4 ERUXovuVaSgB4AEBa6KFAiTyi7o Figure 2A (comment: cohesion protection defect) ------- COMMENT: 153ada5377adf0b4 5 Mc1Dl/8ePjG+W3BQIevC1vaEvqw Figure 2D ------- COMMENT: 153ada5377adf0b4 6 fbKAXm6yZoT6FxzhSDpANk868Kc Figure 3AB vw repurposed this as it was essentially the same as the other annotation to this genotype (suggesting that Rec8-2A was properly expressed but not protected at centromeres during anaphase I.) ------- COMMENT: 153ada5377adf0b4 7 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 153ada5377adf0b4 9 fYqQOpzikopc+3AWAEPEUkYII7w figure 4 ------- COMMENT: 153ada5377adf0b4 10 3aDTqS0iJ6SOFnf3Oqhz1gXi+pk Figure 3BC Strikingly, the phenotype of rec8-2E was completely suppressed by sgo1Δ indicating that Rec8-2E was protected by Sgo1 not only at centromeres but also along the chromosome arm. ------- COMMENT: 153ada5377adf0b4 12 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 153ada5377adf0b4 14 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 153ada5377adf0b4 15 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 153ada5377adf0b4 16 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 153ada5377adf0b4 17 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 153ada5377adf0b4 18 +GqsI7TevIf/6T9srCAzU3HZ9rI refer to model in figure 4. Therefore, consistent with the genetic analyses, this biochemical analysis supports the notion that phosphorylation at Rec8-S450 and the adjacent site plays a role in promoting the PP2A-dependent removal of CK1-dependent phosphorylation of Rec8 (Fig. 4B). ------- COMMENT: 153ada5377adf0b4 20 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 153ada5377adf0b4 21 2J9D8Gu1gJ1l1r77W0bxz1Wf8bc Figure 2F ------- COMMENT: 153ada5377adf0b4 22 WmevStsNZ6N+zB+oeVU7EC21J4M Figure 3D. (comment: phosphomimetic rec8) ------- COMMENT: 153ada5377adf0b4 23 9LIIjvTrnT4XGvweo/5hC3w1nKY Figure 3D. ------- COMMENT: 153ada5377adf0b4 24 9LIIjvTrnT4XGvweo/5hC3w1nKY Figure 3D. ------- COMMENT: 153ada5377adf0b4 25 WmevStsNZ6N+zB+oeVU7EC21J4M Figure 3D. (comment: phosphomimetic rec8) ------- COMMENT: 153ada5377adf0b4 26 wiPBAb2ypzQ8HFKdsOTJgVUnosI To further examine this possibility, we reconstituted Rec8 dephosphorylation in vitro using immunoprecipitated Par1-containing PP2A complexes. ------- COMMENT: 153ada5377adf0b4 27 wiPBAb2ypzQ8HFKdsOTJgVUnosI To further examine this possibility, we reconstituted Rec8 dephosphorylation in vitro using immunoprecipitated Par1-containing PP2A complexes. ------- COMMENT: 153ada5377adf0b4 28 6TfYMwJcg51wiyGZZ0+akYX2JFk figure 4 (comment: no rescue by sgo3) ------- COMMENT: 153ada5377adf0b4 30 fYqQOpzikopc+3AWAEPEUkYII7w Figure 4 ------- COMMENT: 155dba22ce11dc4f 1 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 155dba22ce11dc4f 4 0RLnSAlDpGGGgoiTHlmTc08Ve5E After cultivation in low glucose MM for 10 h, medium was replaced with low-glucose nitrogen-starved MM, and cells were further cultivated for 4 h. ------- COMMENT: 155dba22ce11dc4f 9 enj4cDR4rz300mte38jViKDp4is top panel, Fig. 6A; Fig. S3B,C). ------- COMMENT: 155dba22ce11dc4f 12 eYsAauf3XMkLmmUyqVCmKAqSe58 Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by sst4 mutations (deletion, W170STOP, and Q202STOP). ------- COMMENT: 155dba22ce11dc4f 13 0oY2mmBTAHtKXgJiPl4yZ2ydp7w Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by hse1 mutations (deletion, G253C-primary_transcript) ------- COMMENT: 155dba22ce11dc4f 14 x2zgNZ3nf0MAj3FQZWyJVKTwHwo Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by sst6 mutations (deletion, Q207STOP, G96T-primary_transcript) ------- COMMENT: 155dba22ce11dc4f 15 5+7K9YPvtbI9Odc6N0/2N/Pj3ho Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by vps25 mutations (deletion, W113STOP) ------- COMMENT: 155dba22ce11dc4f 16 ex/YBIHZ6WtIm7Q+s857ypl3+Vs Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by bro1 mutations (deletion, E644STOP) ------- COMMENT: 155dba22ce11dc4f 17 kSVXEoXhRCh3IKjZyfjV3yhkgJ0 Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by aly3/SPCC584.15c mutations (deletion, R457STOP) ------- COMMENT: 155dba22ce11dc4f 18 voE6ffAXbVkj4auRVJHB2X/+t3M Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by tsc1 mutations (deletion, Q688STOP) ------- COMMENT: 155dba22ce11dc4f 19 NklApw8yqIvbrZOXChGw4dIWLC0 Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by tsc2 mutations (deletion, L636STOP) ------- COMMENT: 155dba22ce11dc4f 20 QtFRg+OogQMEzo/x0PrViKyc8xM Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by gtr1 mutations (deletion, C58F) ------- COMMENT: 155dba22ce11dc4f 21 qIMUoR2Rh0ZXt2mVO8sYm4BZRMg Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by npr2 mutations (deletion, Q135K) ------- COMMENT: 155dba22ce11dc4f 22 /B3fPpY4aeWlN7yLA75C3HeSqSw Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by tip41 mutations (deletion, R165L) ------- COMMENT: 155dba22ce11dc4f 23 6I7/gOjPEogVQMGpd0x++xRV2qc Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by not2 mutations (deletion, E31STOP, G849C-primary_transcript) ------- COMMENT: 155dba22ce11dc4f 24 dDrY3friU9hMAHjeJ9LqvyxJijA Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by not3 mutations (deletion, A109C-primary_transcript) ------- COMMENT: 155dba22ce11dc4f 25 Y8gJyG7N0uYyKdEa4auS2HrhjTc Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by puf2 mutations (deletion, Q40STOP, Y416STOP, R751P, R751C, I810S) ------- COMMENT: 155dba22ce11dc4f 26 sDCRAJ0KU397JicRL/UcHaOaeWw Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by ppk14 mutations (deletion, R148STOP, D404E, R469L) ------- COMMENT: 155dba22ce11dc4f 27 MXyO/5bPqOuR3JjS3jFn+ipV8jw Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by ppk15 mutations (deletion, N104T, K259N) ------- COMMENT: 155dba22ce11dc4f 28 VfEVYx7qnxEIy2JSQmtOc/MMUUM Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by prs5 mutations (deletion, L23I) ------- COMMENT: 155dba22ce11dc4f 29 SUeTLD6BAJmFFsDX0S/Y5rDGoiI Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by SPBC16H5.12c mutations (deletion, L67P) ------- COMMENT: 155dba22ce11dc4f 30 0b268UT9pA89E0d8R3fFFZCpS6A Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by pcs2 mutations (deletion, N147D) ------- COMMENT: 155dba22ce11dc4f 31 KWOuOD/yY11DUM/QgvBQg8R1/RI Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by SPAC1296.01c mutations (deletion, Q175STOP) ------- COMMENT: 155dba22ce11dc4f 32 aKwps/3xXnOOF1uv/w4OHDgZ2LU Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by SPAC630.07c mutations (deletion, P278T) ------- COMMENT: 155dba22ce11dc4f 39 DyK04jVC5CR+XJXlBf6jNgieKoY Cytoplasmic Ght5-GFP was observed within the vacuolar membrane stained with FM4-64. ------- COMMENT: 155dba22ce11dc4f 40 6MyV/iv2R9tenGc+/GQCulBXosc Decreased cell proliferation of gad8ts-S240P mutant on low glucose YES solid medium was suppressed by osh6/SPCC23B6.01c mutations (deletion, S117STOP) ------- COMMENT: 155dba22ce11dc4f 41 EH+GAK6D5xOnvUIOmKYAu+8mXq4 Fig1 (comment: aly3 rescues) ------- COMMENT: 155dba22ce11dc4f 46 RvJ0A4lAg3q8AzP/+S0cXGxf3Mo Proliferation defect of gad8ts aly1 mutant in low glucose was similar to that of gad8ts mutant. ------- COMMENT: 155dba22ce11dc4f 47 G3xcFK10kkcYmXOTHaYMc3/X4lA Proliferation defect of gad8ts aly2 mutant in low glucose was similar to that of gad8ts mutant. ------- COMMENT: 155dba22ce11dc4f 48 /+KfGlNfvQA+s4TfAQpdeWcwo3w Proliferation defect of gad8ts rod1 mutant in low glucose was similar to that of gad8ts mutant. ------- COMMENT: 155dba22ce11dc4f 49 OmrIqjg23nEl7d68Z6ezKmK6S9o Proliferation defect of gad8ts mutant in low glucose was restored by SPCC584.15c deletion. ------- COMMENT: 155dba22ce11dc4f 54 l+f1R919mLn1hkTFl9hvZaQF59A fig 6 ------- COMMENT: 155dba22ce11dc4f 55 l+f1R919mLn1hkTFl9hvZaQF59A fig 6 ------- COMMENT: 155dba22ce11dc4f 56 XYXpiZEVvGuXrgNPHHX6YBq9PDo fig 6 (comment: phenocopies WT) ------- COMMENT: 155dba22ce11dc4f 58 ozLd7pck0j2KQtSfeFKiam+mhw4 (comment: although not shown directly , genetic interactions are consistent with this activity) ------- COMMENT: 157f44cabecf9aac 1 RLjaEEICw5cgTVs2oNvBvUzQO1Q binds with high affinity to diverged S. pombe telomeric repeats) ------- COMMENT: 157f44cabecf9aac 2 oF/7Dfa25CjF8+DvjtRbaR1oTV8 binds with high affinity to mammalian-type 5'-TTAGGG-3' telomeric repeats, and with very low affinity to diverged S. pombe telomeric repeats ------- COMMENT: 15a1a89e5c2409e9 17 bhg/X1AiAaK0nZCI5rbJrnC7Fic ------- COMMENT: 15a1a89e5c2409e9 18 bhg/X1AiAaK0nZCI5rbJrnC7Fic ------- COMMENT: 15a1a89e5c2409e9 30 oYwru0c3rSsjHVlfzfAMg/igN7c (comment: request and use GO:new positive regulation of (MF) microfilament motor activity instead? depends on ancestry of motor activity branch) ------- COMMENT: 15a1a89e5c2409e9 48 mOKYnP93XRP47oD0P+r2wODKzBQ (comment: CONDITION 25 degrees C, i.e. lower end of normal temp. range; penetrance higher at 29 degrees C) ------- COMMENT: 15a5190fe0560730 1 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 15a5190fe0560730 2 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 15a5190fe0560730 6 DSU1qtrpPJCAnFfEBISuHhpVgOo Fig. 1A, Inset and Movie S1/ number of Mcp5 molecules per cluster 10 ± 2 ( Fig. 1D) ------- COMMENT: 15a5190fe0560730 7 yMkdMm/hUWIoztiEGhiP7eJsoBE Fig 2 & Fig. 3C ------- COMMENT: 15a5190fe0560730 8 dKEmVsbaMXIitPIxWW2nfEI+SRM Fig. 4 D–F ------- COMMENT: 15a5190fe0560730 10 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 15a5190fe0560730 11 Wdl68rmxa183plF2uXM6i1fP5n8 Fig. 5B A (comment: ALSO FOR THE myo-1TH3 domain deletion- need genotype description) ------- COMMENT: 15a5190fe0560730 12 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 15a5190fe0560730 13 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 15a5190fe0560730 14 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 16267d2a11f0cdbf 24 hhQeDv0Q0aIfnjn6akEEbsi44C4 (comment: cmk1 seems to phosphorylate prz1 which makes prz1 cytoplasmic. During response to calcium, calcineurin dephosphorylates prz1 and it goes to the nucleus.) ------- COMMENT: 16267d2a11f0cdbf 41 SwvReMtdinyCUaoZ0jQ+/K59fWY ------- COMMENT: 16267d2a11f0cdbf 57 aJiBoN8VE2fDBMwHyPID9SFsDm0 (comment: prz1 is the pombe equivalent to NFAT -functional equivalent rather than ortholog) ------- COMMENT: 16267d2a11f0cdbf 65 aJiBoN8VE2fDBMwHyPID9SFsDm0 (comment: prz1 is the pombe equivalent to NFAT -functional equivalent rather than ortholog) ------- COMMENT: 163961d04bcaf919 25 m+msjljkNuwyJ/sb4DED36l5iik (comment: consensus recognition sequence 5'-TCG(G/C)(A/T)xxTTxAA) ------- COMMENT: 163961d04bcaf919 30 m+msjljkNuwyJ/sb4DED36l5iik (comment: consensus recognition sequence 5'-TCG(G/C)(A/T)xxTTxAA) ------- COMMENT: 166679a73087715a 1 1WOLHBx368TY33Likp3vNGDnl+A A tagged version of Cbp7 was generated and found to be highly enriched in purified mitochondria (Figure 2B) ------- COMMENT: 166679a73087715a 2 s4a9P421NI94oekHETVe5IYht+Q (Figure 2C) ------- COMMENT: 166679a73087715a 3 ABbMaimIK+q89eXwcZpWDh5m9cw Cbp7 deletion prevented growth on non-fermentable medium in both contexts (Figure 2C and not shown) ------- COMMENT: 166679a73087715a 4 nQ8RM4AFJ3kzAY324OgT9AfNDII but grew normally on fermentable medium containing an- timycin, showing that the ATP synthase (complex V) is not strongly affected by the mutation (Figure 2C) ------- COMMENT: 166679a73087715a 5 myWnMZ/8isdUvoK8KIc3J3Rmovc Cytochrome spectra showed strongly decreased peaks for cytochromes b and c1 of complex III but normal to increased absorbance for cytochrome a + a3 of complex IV (Figure 2D). ------- COMMENT: 166679a73087715a 6 iNv3xUuA1KEG/VfG4JcMD10YiIs First, the cytb mRNA was significantly, although never fully, destabi- lized (Figure 2F) whereas other mRNAs were not affected. ------- COMMENT: 166679a73087715a 7 KsFDCusY43x+K/XqvQi6quckUR8 Cytb showed a clear pattern of degrada- tion over a 20 h chase in the wt, but appeared repeatedly re- sistant to degradation in the Δcbp7 mutant in several inde- pendent experiments (Figure 2H). This unexpected degra- dation pattern could indicate that in Δcbp7 cells, the newly- synthetized Cytb might escape recognition and/or acces- sibility by mitochondrial proteases (65), possibly because of misfolding, aggregation and/or defective membrane in- sertion. ------- COMMENT: 166679a73087715a 8 lJW7IQg+2oQ86xkzjmZukK2FvG4 This absence of degradation was surprising since Cytb steady state seemed dramatically decreased in the mu- tant compared to the wt, as revealed in purified mitochon- dria (Figure 2E) as well as in the total protein chase sam- ples (Supplementary Figure S3) using our in-house anti- body. We have no definitive solution to this paradox; the answer might lie with a particularity of our Cytb antibody. This only recognizes Cytb in non-boiled samples, suggest- ing that the epitope recognised by the antibody is a small structural motif, rather than a linear sequence. Thus, if the neo-synthesized Cytb cannot be correctly folded and in- serted into the membrane, the epitope recognised by our antibody may no longer be present. ------- COMMENT: 166679a73087715a 9 UCOwUB7iIBBeew0xz7arEY8Xt1Q Whereas Cytb itself was never enriched in IPs of Cbp7-cMyc compared to wt, a pro- tein of unknown function, Spac22h10.09, was strongly and significantly enriched in all experiments (Figure 3A) ------- COMMENT: 166679a73087715a 10 5DpwxDpaAIuxrhWLOvPtEzkv104 In order to confirm the interaction between Cbp7 and Cbp8, we FLAG-tagged Cbp8 in a Cbp7-cMyc background and showed that it is a mitochondrial protein (Figure 3B); then we carried out IPs of Cbp8-FLAG and Cbp7-cMyc and observed in each case a clear co-immunoprecipitation of the other tagged protein (Figure 3C). ------- COMMENT: 166679a73087715a 11 o77iIpixI5WejlSFfJ08bWSh0UQ The deletion of the cbp8 gene in both i and Δi back- grounds yielded respiratory deficient but antimycin resis- tant cells showing that ATP synthase was not affected (Figure 3D and not shown). ------- COMMENT: 166679a73087715a 12 o77iIpixI5WejlSFfJ08bWSh0UQ The deletion of the cbp8 gene in both i and Δi back- grounds yielded respiratory deficient but antimycin resis- tant cells showing that ATP synthase was not affected (Figure 3D and not shown). ------- COMMENT: 166679a73087715a 13 o77iIpixI5WejlSFfJ08bWSh0UQ The deletion of the cbp8 gene in both i and Δi back- grounds yielded respiratory deficient but antimycin resis- tant cells showing that ATP synthase was not affected (Figure 3D and not shown). ------- COMMENT: 166679a73087715a 14 8x3j/LA54hT9FRcVckTv/NaRm54 In brief, Δcbp8 looked similar to Δcbp7 since complex III was barely detectable in BN-PAGE, cytochrome b and c1 were not spectrally detected and a low level of cytb RNA was ob- served; ------- COMMENT: 166679a73087715a 15 KPbQFc6qIFrWtj1UgU+bUwrrPQM However, as with Δcbp7 cells, the newly synthesized Cytb protein showed an aber- rant degradation pattern compared to the wild-type since it remained stable for up to 20 h in a chase experiment (Sup- plementary Figure S5). ------- COMMENT: 166679a73087715a 19 ABbMaimIK+q89eXwcZpWDh5m9cw Cbp7 deletion prevented growth on non-fermentable medium in both contexts (Figure 2C and not shown) ------- COMMENT: 166679a73087715a 20 ABbMaimIK+q89eXwcZpWDh5m9cw Cbp7 deletion prevented growth on non-fermentable medium in both contexts (Figure 2C and not shown) ------- COMMENT: 166679a73087715a 21 ABbMaimIK+q89eXwcZpWDh5m9cw Cbp7 deletion prevented growth on non-fermentable medium in both contexts (Figure 2C and not shown) ------- COMMENT: 166679a73087715a 22 ABbMaimIK+q89eXwcZpWDh5m9cw Cbp7 deletion prevented growth on non-fermentable medium in both contexts (Figure 2C and not shown) ------- COMMENT: 166679a73087715a 23 ABbMaimIK+q89eXwcZpWDh5m9cw Cbp7 deletion prevented growth on non-fermentable medium in both contexts (Figure 2C and not shown) ------- COMMENT: 166679a73087715a 24 ab7zKs0tq7eEKQ1H6UoGJ9vH1Sk The strength of suppression by the mug178 or ppr7 plasmids was similar in terms of respiratory growth and cytochrome spectra (Figure 5A, B). ------- COMMENT: 166679a73087715a 25 ab7zKs0tq7eEKQ1H6UoGJ9vH1Sk The strength of suppression by the mug178 or ppr7 plasmids was similar in terms of respiratory growth and cytochrome spectra (Figure 5A, B). ------- COMMENT: 166679a73087715a 27 RJxRmIgrfwGPGMrM267U0Xjq5hM High copy suppres- sors were also searched for starting from the Δcbp8 deletion and identified the same genes (Table 2): mug178, ppr7 and zfs1, reinforcing the idea that Cbp7 and Cbp8 are acting at the same step of Cytb biogenesis. ------- COMMENT: 166679a73087715a 28 RJxRmIgrfwGPGMrM267U0Xjq5hM High copy suppres- sors were also searched for starting from the Δcbp8 deletion and identified the same genes (Table 2): mug178, ppr7 and zfs1, reinforcing the idea that Cbp7 and Cbp8 are acting at the same step of Cytb biogenesis. ------- COMMENT: 166679a73087715a 30 NRkC7ROtUtVqKKoLbsEEWEijkPI First, fractiona- tions of mitochondria and post-mitochondrial supernatant in a double tagged strain showed that both are indeed mitochondrial proteins (Figure 6C). Second, western blot analysis of mitochondrial extracts from the double tagged strain fractionated on EDTA-sucrose gradients to separate both ribosomal subunits, showed that both tagged proteins co-sediment with the small ribosomal protein mS45 (Fig- ure 6D). Third, immunoprecipitations performed under EDTA conditions showed that both Mrp51 and Mug178 did co-immunoprecipitate mS45 but not mL58 (Figure 6E). ------- COMMENT: 166679a73087715a 31 NRkC7ROtUtVqKKoLbsEEWEijkPI First, fractiona- tions of mitochondria and post-mitochondrial supernatant in a double tagged strain showed that both are indeed mitochondrial proteins (Figure 6C). Second, western blot analysis of mitochondrial extracts from the double tagged strain fractionated on EDTA-sucrose gradients to separate both ribosomal subunits, showed that both tagged proteins co-sediment with the small ribosomal protein mS45 (Fig- ure 6D). Third, immunoprecipitations performed under EDTA conditions showed that both Mrp51 and Mug178 did co-immunoprecipitate mS45 but not mL58 (Figure 6E). ------- COMMENT: 166679a73087715a 32 RJxRmIgrfwGPGMrM267U0Xjq5hM High copy suppres- sors were also searched for starting from the Δcbp8 deletion and identified the same genes (Table 2): mug178, ppr7 and zfs1, reinforcing the idea that Cbp7 and Cbp8 are acting at the same step of Cytb biogenesis. ------- COMMENT: 166679a73087715a 33 ab7zKs0tq7eEKQ1H6UoGJ9vH1Sk The strength of suppression by the mug178 or ppr7 plasmids was similar in terms of respiratory growth and cytochrome spectra (Figure 5A, B). ------- COMMENT: 166679a73087715a 34 ncP78HYfhIwxdR7e9RAF5oOsmSo The complete absence of Mrp51 was un- viable in a wild-type background. This is expected for an es- sential mitoribosomal protein because S. pombe is a petite- negative yeast, i.e. that cannot tolerate the complete loss of the mtDNA, or similarly a complete block in mitochon- drial translation ------- COMMENT: 166679a73087715a 35 jVo4XEl0tJhCGUE0/8QIbWsQO8o The mrp51 deletion could be obtained in the ptp1-1 strain, which allows S. pombe to remain alive without mtDNA (47), ------- COMMENT: 166679a73087715a 36 7qbd0gzicszw7PoWS2JtFk32BSk On the contrary, the deletion of mug178 yielded viable cells. ------- COMMENT: 166679a73087715a 37 wNlV5e3AW7V4UyeeaQ2NMjZxsfs The Δmug178 mutant was unable to grow on non-fermentable medium and lacked spectrally detectable cytochromes b and c1 but was not sensitive to antimycin A, showing that ATP synthase is not defective (Figure 7A-B). ------- COMMENT: 166679a73087715a 38 7UxP/D9GmLbcmMfYj49d9p+vsyg but was not sensitive to antimycin A, showing that ATP synthase is not defective (Figure 7A-B) ------- COMMENT: 166679a73087715a 39 Bq75LVFWjnthV8UMhlbeqGFHFJ8 (comment: ABOLISHED PROTEIN SPECIFIC TRANSLATION) Radioactive label- ing of newly translated mitochondrial proteins showed that the combination of Δmug178 with either Δcbp7 or Δcbp8 abolished cytb translation while Δcbp7 Δcbp8 was similar to Δcbp8 (Figure 8B) ------- COMMENT: 166679a73087715a 40 Bq75LVFWjnthV8UMhlbeqGFHFJ8 (comment: ABOLISHED PROTEIN SPECIFIC TRANSLATION) Radioactive label- ing of newly translated mitochondrial proteins showed that the combination of Δmug178 with either Δcbp7 or Δcbp8 abolished cytb translation while Δcbp7 Δcbp8 was similar to Δcbp8 (Figure 8B) ------- COMMENT: 166679a73087715a 41 5tftVYGVytZXahZr4K1q3GAIeck Second, we found that growth of Δmug178 cells on non-fermentable medium was restored by the ppr4, mrh5 or mrp51 suppressor alleles of Δcbp7 (Supplementary Figure S10A). ------- COMMENT: 166679a73087715a 42 5tftVYGVytZXahZr4K1q3GAIeck Second, we found that growth of Δmug178 cells on non-fermentable medium was restored by the ppr4, mrh5 or mrp51 suppressor alleles of Δcbp7 (Supplementary Figure S10A). ------- COMMENT: 166679a73087715a 43 5tftVYGVytZXahZr4K1q3GAIeck Second, we found that growth of Δmug178 cells on non-fermentable medium was restored by the ppr4, mrh5 or mrp51 suppressor alleles of Δcbp7 (Supplementary Figure S10A). ------- COMMENT: 166679a73087715a 44 AtonJIEbultI1DAUzdWgRLgmm5U We found that Δmrh5 was essential for respiratory growth, even in Δi background, suggesting that if Mrh5 is involved in intron excision, it also has another role. ------- COMMENT: 166679a73087715a 45 19CFPugpVm2oYRoCpDtXdX9QhYw The Δmrh5 mutation did not impair growth on antimycin con- taining medium, showing that ATPase activity is not af- fected (Figure 9A). ------- COMMENT: 166679a73087715a 46 TF8ve7keixQKsFvUwT8mp6kSohw Western blot analysis was performed on the Δmrh5 and mrh5-G230E mutants, as well as the tagged Mrh5-cMyc strain (Figure 9C). The results showed that Mrh5 is a mitochondrial protein and that Cox1 and Cox2 were undetectable in both mutants. ------- COMMENT: 166679a73087715a 47 TF8ve7keixQKsFvUwT8mp6kSohw Western blot analysis was performed on the Δmrh5 and mrh5-G230E mutants, as well as the tagged Mrh5-cMyc strain (Figure 9C). The results showed that Mrh5 is a mitochondrial protein and that Cox1 and Cox2 were undetectable in both mutants. ------- COMMENT: 166679a73087715a 48 TF8ve7keixQKsFvUwT8mp6kSohw Western blot analysis was performed on the Δmrh5 and mrh5-G230E mutants, as well as the tagged Mrh5-cMyc strain (Figure 9C). The results showed that Mrh5 is a mitochondrial protein and that Cox1 and Cox2 were undetectable in both mutants. ------- COMMENT: 166679a73087715a 49 TF8ve7keixQKsFvUwT8mp6kSohw Western blot analysis was performed on the Δmrh5 and mrh5-G230E mutants, as well as the tagged Mrh5-cMyc strain (Figure 9C). The results showed that Mrh5 is a mitochondrial protein and that Cox1 and Cox2 were undetectable in both mutants. ------- COMMENT: 166679a73087715a 50 ZMR2idno3xZL8+1Zu9SXMhB1q18 Cytb level was partly decreased in Δmrh5 and only slightly in the point mutant. ------- COMMENT: 166679a73087715a 51 ZMR2idno3xZL8+1Zu9SXMhB1q18 Cytb level was partly decreased in Δmrh5 and only slightly in the point mutant. ------- COMMENT: 166679a73087715a 52 0ZMbqP9vE8aFlrWRACHtZCGeSe0 (comment: ABOLISHED PROTEIN SPECIFIC TRANSLATION) Whereas newly-synthesized Cox2 and Cox3 were radioac- tively labeled, Cox1 synthesis was abolished although the mature cox1 mRNA was only slightly decreased in Δi cells, showing that Mrh5 is required for cox1 translation. ------- COMMENT: 166679a73087715a 53 Tekpe57rDQQLkpnPGvFKpC92Cmk In i cells, the mature cox1 mRNA was absent in Δmrh5 and the precursor RNA containing both cox1 introns accumu- lated; ------- COMMENT: 166679a73087715a 54 Tekpe57rDQQLkpnPGvFKpC92Cmk In i cells, the mature cox1 mRNA was absent in Δmrh5 and the precursor RNA containing both cox1 introns accumu- lated; ------- COMMENT: 166679a73087715a 57 AKRlGY4gqsG04Cp+NbOQYYbrwmA SMALL SUBUNIT In i cells, the mature cox1 mRNA was absent in Δmrh5 and the precursor RNA containing both cox1 introns accumu- lated; in the point mutant, partial splicing deficiency was observed. ------- COMMENT: 166679a73087715a 62 FpcC4DdLIViQexjMBg43TiaF8Eg The strongest interactors (in red) were Ppr4 and Mtf2 as well as the S. pombe homolog of the S. cerevisiae Sls1 pro- tein. Figure 10 ------- COMMENT: 166679a73087715a 63 FpcC4DdLIViQexjMBg43TiaF8Eg The strongest interactors (in red) were Ppr4 and Mtf2 as well as the S. pombe homolog of the S. cerevisiae Sls1 pro- tein. Figure 10 ------- COMMENT: 166679a73087715a 64 FpcC4DdLIViQexjMBg43TiaF8Eg The strongest interactors (in red) were Ppr4 and Mtf2 as well as the S. pombe homolog of the S. cerevisiae Sls1 pro- tein. Figure 10 ------- COMMENT: 166679a73087715a 65 5KquCXoAbNMgQMsTK8+RyzJVORE In parallel, the same IP and LC–MS/MS experiment was carried out with the tagged Ppr4-cMyc strain. The raw re- sults presented in Supplementary Table S3 and statistically analyzed (Supplementary Table S5) were also made into volcano plots (Figure 10B). Ppr4 strongly interacted with Mrh5, Mtf2 and Sls1 and there was also a slight enrich- ment in some ribosomal proteins. Retrieving the same four highly enriched proteins starting from either Mrh5-cMyc and Ppr4-cMyc is a strong argument for the existence of a stable Mrh5/Ppr4/Mtf2/Sls1 complex. ------- COMMENT: 166679a73087715a 66 5KquCXoAbNMgQMsTK8+RyzJVORE In parallel, the same IP and LC–MS/MS experiment was carried out with the tagged Ppr4-cMyc strain. The raw re- sults presented in Supplementary Table S3 and statistically analyzed (Supplementary Table S5) were also made into volcano plots (Figure 10B). Ppr4 strongly interacted with Mrh5, Mtf2 and Sls1 and there was also a slight enrich- ment in some ribosomal proteins. Retrieving the same four highly enriched proteins starting from either Mrh5-cMyc and Ppr4-cMyc is a strong argument for the existence of a stable Mrh5/Ppr4/Mtf2/Sls1 complex. ------- COMMENT: 166679a73087715a 67 5KquCXoAbNMgQMsTK8+RyzJVORE In parallel, the same IP and LC–MS/MS experiment was carried out with the tagged Ppr4-cMyc strain. The raw re- sults presented in Supplementary Table S3 and statistically analyzed (Supplementary Table S5) were also made into volcano plots (Figure 10B). Ppr4 strongly interacted with Mrh5, Mtf2 and Sls1 and there was also a slight enrich- ment in some ribosomal proteins. Retrieving the same four highly enriched proteins starting from either Mrh5-cMyc and Ppr4-cMyc is a strong argument for the existence of a stable Mrh5/Ppr4/Mtf2/Sls1 complex. ------- COMMENT: 166679a73087715a 68 VtoHbyIEVEANsPI9uvpk1Nplt1U Δsls1 cells lacked spectral a+a3 peak, were devoid of Cox1 and very depleted in Cox2 (Figure 11B, C). ------- COMMENT: 166679a73087715a 69 VtoHbyIEVEANsPI9uvpk1Nplt1U Δsls1 cells lacked spectral a+a3 peak, were devoid of Cox1 and very depleted in Cox2 (Figure 11B, C). ------- COMMENT: 166679a73087715a 70 DCO0nf39Om0cBMIrI28RkHTkWNc In i cells, the mature cox1 mRNA was absent in Δmrh5 and the precursor RNA containing both cox1 introns accumu- lated; in the point mutant, partial splicing deficiency was observed. ------- COMMENT: 166679a73087715a 71 bJNM4bdWnm4uMvuXr4LceAlVI2A Thus, our mass spectrometry and phenotypic analyses show that Mrh5, Ppr4, Mtf2 and Sls1 are part of a cox1 translational complex interacting with the mitoribosome, at least through Mrh5. ------- COMMENT: 166679a73087715a 72 bJNM4bdWnm4uMvuXr4LceAlVI2A Thus, our mass spectrometry and phenotypic analyses show that Mrh5, Ppr4, Mtf2 and Sls1 are part of a cox1 translational complex interacting with the mitoribosome, at least through Mrh5. ------- COMMENT: 166679a73087715a 73 bJNM4bdWnm4uMvuXr4LceAlVI2A Thus, our mass spectrometry and phenotypic analyses show that Mrh5, Ppr4, Mtf2 and Sls1 are part of a cox1 translational complex interacting with the mitoribosome, at least through Mrh5. ------- COMMENT: 1695e0f67f5e2639 32 C8alWXCwuLJ8/aKnqjq2sDti/8A (comment: CONDITION Southern blot) (comment: same as rap1-7A single mutant) ------- COMMENT: 16ad711046a3f839 13 MIC1mV7yy0CDCDRJjU+iadOOgdY figure 4d ------- COMMENT: 16ad711046a3f839 16 vCZ5/ANd+bkBNZRJfFC3rhm5IwY fig 4e ------- COMMENT: 16ad711046a3f839 19 9EqTzUEeKA/lxBm6wM7vDmYYwy4 figure 3c ------- COMMENT: 16ad711046a3f839 22 acGVpmK5ESo19l4S0TGlsnyofys Supp. S4 ------- COMMENT: 16ad711046a3f839 23 acGVpmK5ESo19l4S0TGlsnyofys Supp. S4 ------- COMMENT: 16ad711046a3f839 40 9uunWMn9suFI6uEbzCwFxrnRngI figure 2 AB inactive separase, uncleavable kleisin ------- COMMENT: 16ad711046a3f839 41 9uunWMn9suFI6uEbzCwFxrnRngI figure 2 AB (comment: inactive separase, uncleavable kleisin) ------- COMMENT: 16ad711046a3f839 42 9uunWMn9suFI6uEbzCwFxrnRngI figure 2 AB (comment: inactive separase, uncleavable kleisin) ------- COMMENT: 16ad711046a3f839 43 9uunWMn9suFI6uEbzCwFxrnRngI figure 2 AB (comment: inactive separase, uncleavable kleisin) ------- COMMENT: 16ad711046a3f839 44 4BvDTb3bzRr+8KTtWeihIYfdmUY figure 1A, Supp S1A ------- COMMENT: 16ad711046a3f839 45 4BvDTb3bzRr+8KTtWeihIYfdmUY figure 1A, Supp S1A ------- COMMENT: 16ad711046a3f839 46 u5NstTm9+D7SOLo8XByDovu2OUk figure 1 A ------- COMMENT: 16ad711046a3f839 47 u5NstTm9+D7SOLo8XByDovu2OUk figure 1 A ------- COMMENT: 16ad711046a3f839 48 u5NstTm9+D7SOLo8XByDovu2OUk figure 1 A ------- COMMENT: 16ad711046a3f839 49 u5NstTm9+D7SOLo8XByDovu2OUk figure 1 A ------- COMMENT: 16ad711046a3f839 50 u5NstTm9+D7SOLo8XByDovu2OUk figure 1 A ------- COMMENT: 16ad711046a3f839 51 u5NstTm9+D7SOLo8XByDovu2OUk figure 1 A ------- COMMENT: 16ad711046a3f839 52 u5NstTm9+D7SOLo8XByDovu2OUk figure 1 A ------- COMMENT: 16ad711046a3f839 53 u5NstTm9+D7SOLo8XByDovu2OUk figure 1 A ------- COMMENT: 16ad711046a3f839 54 u5NstTm9+D7SOLo8XByDovu2OUk figure 1 A ------- COMMENT: 16ad711046a3f839 55 u5NstTm9+D7SOLo8XByDovu2OUk figure 1 A ------- COMMENT: 16ad711046a3f839 56 u5NstTm9+D7SOLo8XByDovu2OUk figure 1 A ------- COMMENT: 16ad711046a3f839 57 1sUygGrndKrxW19aLHPUcs65vXs figure 3, figure S1B ------- COMMENT: 16ad711046a3f839 58 1sUygGrndKrxW19aLHPUcs65vXs figure 3, figure S1B ------- COMMENT: 16ad711046a3f839 59 1sUygGrndKrxW19aLHPUcs65vXs figure 3, figure S1B ------- COMMENT: 16ad711046a3f839 60 1sUygGrndKrxW19aLHPUcs65vXs figure 3, figure S1B ------- COMMENT: 16ad711046a3f839 61 1sUygGrndKrxW19aLHPUcs65vXs figure 3, figure S1B ------- COMMENT: 16ad711046a3f839 62 1sUygGrndKrxW19aLHPUcs65vXs figure 3, figure S1B ------- COMMENT: 16ad711046a3f839 63 1sUygGrndKrxW19aLHPUcs65vXs figure 3, figure S1B ------- COMMENT: 16ad711046a3f839 64 1sUygGrndKrxW19aLHPUcs65vXs figure 3, figure S1B ------- COMMENT: 16ad711046a3f839 65 1sUygGrndKrxW19aLHPUcs65vXs figure 3, figure S1B ------- COMMENT: 16ad711046a3f839 66 1sUygGrndKrxW19aLHPUcs65vXs figure 3, figure S1B ------- COMMENT: 16ad711046a3f839 67 1sUygGrndKrxW19aLHPUcs65vXs figure 3, figure S1B ------- COMMENT: 16ad711046a3f839 68 1sUygGrndKrxW19aLHPUcs65vXs figure 3, figure S1B ------- COMMENT: 16ad711046a3f839 69 1sUygGrndKrxW19aLHPUcs65vXs figure 3, figure S1B ------- COMMENT: 16ad711046a3f839 70 O+ogVpvOU5jD1UOIYSW5k9q1UZE fig 4b ------- COMMENT: 16ad711046a3f839 71 O+ogVpvOU5jD1UOIYSW5k9q1UZE fig 4b ------- COMMENT: 16ad711046a3f839 72 O+ogVpvOU5jD1UOIYSW5k9q1UZE fig 4b ------- COMMENT: 170b4d4db77791d1 8 XSSWE4RMc+oTknaESg2xONEcZ0k fig 3 B WT 10% ------- COMMENT: 1719104d313ca4be 86 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: 1719104d313ca4be 87 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: 1719104d313ca4be 88 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: 1719104d313ca4be 89 j93cH0v59uG8CGRWb6eZPjDR8tk Fig. 3F (comment: loss of mitotic competence) ------- COMMENT: 1719104d313ca4be 91 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 1719104d313ca4be 92 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 1719104d313ca4be 93 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 1719104d313ca4be 94 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 1719104d313ca4be 95 X3fbo8p+FTO7Bcu8pwqR2+l7VHM Fig 4A,E ------- COMMENT: 1719104d313ca4be 96 tEKPG4SG06Sd+MBgYUZdTXf2msI Fig S3 ------- COMMENT: 1719104d313ca4be 97 8Bfe9Cak+Lc8NE4KYnce9AOU+B8 Fig 4E ------- COMMENT: 1719104d313ca4be 98 5zLBcPfE96G7rFQPJBZsaEOa1Og Fig 4C ------- COMMENT: 1719104d313ca4be 99 8Bfe9Cak+Lc8NE4KYnce9AOU+B8 Fig 4E ------- COMMENT: 1719104d313ca4be 100 8Bfe9Cak+Lc8NE4KYnce9AOU+B8 Fig 4E ------- COMMENT: 1719104d313ca4be 101 8Bfe9Cak+Lc8NE4KYnce9AOU+B8 Fig 4E ------- COMMENT: 1719104d313ca4be 102 8Bfe9Cak+Lc8NE4KYnce9AOU+B8 Fig 4E ------- COMMENT: 1719104d313ca4be 103 8Bfe9Cak+Lc8NE4KYnce9AOU+B8 Fig 4E ------- COMMENT: 1719104d313ca4be 104 8Bfe9Cak+Lc8NE4KYnce9AOU+B8 Fig 4E ------- COMMENT: 1719104d313ca4be 105 8Bfe9Cak+Lc8NE4KYnce9AOU+B8 Fig 4E ------- COMMENT: 1719104d313ca4be 106 8Bfe9Cak+Lc8NE4KYnce9AOU+B8 Fig 4E ------- COMMENT: 1719104d313ca4be 107 8Bfe9Cak+Lc8NE4KYnce9AOU+B8 Fig 4E ------- COMMENT: 1719104d313ca4be 108 8Bfe9Cak+Lc8NE4KYnce9AOU+B8 Fig 4E ------- COMMENT: 1719104d313ca4be 109 8Bfe9Cak+Lc8NE4KYnce9AOU+B8 Fig 4E ------- COMMENT: 1719104d313ca4be 110 8Bfe9Cak+Lc8NE4KYnce9AOU+B8 Fig 4E ------- COMMENT: 1719104d313ca4be 111 8Bfe9Cak+Lc8NE4KYnce9AOU+B8 Fig 4E ------- COMMENT: 1719104d313ca4be 112 8Bfe9Cak+Lc8NE4KYnce9AOU+B8 Fig 4E ------- COMMENT: 1719104d313ca4be 203 j93cH0v59uG8CGRWb6eZPjDR8tk Fig. 3F (comment: loss of mitotic competence) ------- COMMENT: 1769f7fc9f807232 4 WI2NuEhQ/iLNDD4ooX2zEv9gn5w Fig 1 A ------- COMMENT: 1769f7fc9f807232 5 WI2NuEhQ/iLNDD4ooX2zEv9gn5w Fig 1 A ------- COMMENT: 1769f7fc9f807232 6 WI2NuEhQ/iLNDD4ooX2zEv9gn5w Fig 1 A ------- COMMENT: 1769f7fc9f807232 7 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 1769f7fc9f807232 8 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 1769f7fc9f807232 9 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 1769f7fc9f807232 10 lPv0FYWNBQwhmYovckt5zRRm4Vc fig 1B ------- COMMENT: 1769f7fc9f807232 11 +ELLjwUfK5aTh8y7xc8m451gJk4 Fig 1 C ------- COMMENT: 1769f7fc9f807232 12 +ELLjwUfK5aTh8y7xc8m451gJk4 Fig 1 C ------- COMMENT: 1769f7fc9f807232 13 uXKhwqRXsfGR8wEgs0+oJLFuxGI Fig 1 E,F ------- COMMENT: 1769f7fc9f807232 14 m7z1baIHGa2fDYSrElOpARBALn0 Supplementary Figure S3B ------- COMMENT: 1769f7fc9f807232 15 I0433nphvSrpYt2wFV7BiWQT7Mk Supplementary Figure S3B ((comment: abolished by galactose addition) ------- COMMENT: 1769f7fc9f807232 16 N6gdfNLkuPfyqKNbJqqQxYyAr9g Supp. S3 ------- COMMENT: 1769f7fc9f807232 17 N6gdfNLkuPfyqKNbJqqQxYyAr9g Supp. S3 ------- COMMENT: 1769f7fc9f807232 20 N6gdfNLkuPfyqKNbJqqQxYyAr9g Supp. S3 ------- COMMENT: 1769f7fc9f807232 21 tEJGzO7J0TVngwLvaow8UAOQOp0 Figure 2B (comment: barely detectable level ) ------- COMMENT: 1769f7fc9f807232 22 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: 1769f7fc9f807232 23 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: 1769f7fc9f807232 24 PIgoBKjg0HrVDS2AIuG8wqnM4Wk Figure 2B, 8B,8C ------- COMMENT: 1769f7fc9f807232 25 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: 1769f7fc9f807232 38 R9bg/JAzowvcTG25avXkCq3TBGM figure 6 c ------- COMMENT: 1769f7fc9f807232 39 R9bg/JAzowvcTG25avXkCq3TBGM figure 6 c ------- COMMENT: 1769f7fc9f807232 40 PKKClEb5YvfUv3yoSQYBgiC/qvE figure 6 a ------- COMMENT: 1769f7fc9f807232 41 PKKClEb5YvfUv3yoSQYBgiC/qvE figure 6 a ------- COMMENT: 1769f7fc9f807232 42 cWOc1PXhV78eewyU4+tR1ureLPw Fig 7 A ------- COMMENT: 1769f7fc9f807232 44 cWOc1PXhV78eewyU4+tR1ureLPw Fig 7 A ------- COMMENT: 1769f7fc9f807232 45 UnQw94nU5T+QzGDesDT6Zls7XHA fig 7 B ------- COMMENT: 1769f7fc9f807232 46 8p07G+2y16zQtZOd8dzgBOec7Ek Fig 7 c ------- COMMENT: 1769f7fc9f807232 47 yFupPf9bHFYb8rpZ6MiFwW9UgKA Supplementary Figure S7 ------- COMMENT: 1769f7fc9f807232 48 lPv0FYWNBQwhmYovckt5zRRm4Vc fig 1B ------- COMMENT: 1769f7fc9f807232 49 UoWggpjc06sdye9g7iqB+rK6O6c figure 8 A ------- COMMENT: 1769f7fc9f807232 50 h2eG99X29tbPR8SS4xvFsTzB8uw Figure 8B,8C (comment: barely detectable level ) ------- COMMENT: 1769f7fc9f807232 51 i2FjbkihTnHVhJipgKSEaN4ukN4 Figure 8B,8C ------- COMMENT: 1769f7fc9f807232 52 i2FjbkihTnHVhJipgKSEaN4ukN4 Figure 8B,8C ------- COMMENT: 1769f7fc9f807232 54 nXnxx20qvRXwifxho/RCx4NSaMo figure 8 D ------- COMMENT: 1769f7fc9f807232 55 5wLR6J/rVUqWtkuAAMoZfEOGryM figure 9A ------- COMMENT: 1769f7fc9f807232 56 bumytpxFjafghn0w4B/yJZcnJTY fig 9C ------- COMMENT: 1769f7fc9f807232 57 d78vAamQDsFuqLnuCIeDPZSqyv8 figure Supp S8 ------- COMMENT: 1769f7fc9f807232 58 QfAwvzH1v+p+F0Wr/WCL3UtqMJ4 figure 9 d ------- COMMENT: 1769f7fc9f807232 59 UNHLpW9RzadR1rb293QHXAxs2x0 (comment: Based on what we know about lon in other species, i think the rescue of mpa1 phenotype is enough to predict a catabolic role) ------- COMMENT: 1769f7fc9f807232 60 ceEf4d5N6u/gj6rLtsKeBJQe7hE figure 9 F ------- COMMENT: 1769f7fc9f807232 61 ceEf4d5N6u/gj6rLtsKeBJQe7hE figure 9 F ------- COMMENT: 17a6a3be58a90b9c 1 /uY9l8p2BBIQO2aGF/iylgViW30 (comment: minor ------- COMMENT: 17a6a3be58a90b9c 2 GMnUYh72wxE84flgNG12A+iEBug (comment: major) ------- COMMENT: 17a6a3be58a90b9c 17 +b/xX76WPZ5asu/MFq9nej2Tclo Like the gms1D mutant, neither the uge1D strain nor the uge1Dgal10D strain reacted with PNA (Fig. 2b ------- COMMENT: 17bd4722f4e3d0a7 1 PQz1bg7ipbzS1O+aQruooc1j83o (comment: Phosphorylation releases MBF from DNA and represses transcription of MBF-dependent genes.) ------- COMMENT: 17bd4722f4e3d0a7 2 J1X8a/Lf3m8Tg+kQuxl/ux+YrE0 (comment: annotate - Serine 114 and Threonine 115 are phosphorylated by Cds1 upon activation of the DNA replication checkpoint) (comment: Yox1 phosphorylation by Cds1 releases Yox1 from MBF and activates MBF-dependent transcription) ------- COMMENT: 17bd4722f4e3d0a7 22 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig 4 ------- COMMENT: 17bd4722f4e3d0a7 24 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig 4 ------- COMMENT: 17bd4722f4e3d0a7 26 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig 4 ------- COMMENT: 17bd4722f4e3d0a7 40 +rAdDWWKWKeJBtA2mX1t+NFlT1g (comment: localizes the MBF complex) ------- COMMENT: 17cceecf86230427 5 FQd4mK4OSZZi3AAzsR6Hd92s/Ng Figure 4A and Supplemental Figure S4 ------- COMMENT: 17cceecf86230427 6 FQd4mK4OSZZi3AAzsR6Hd92s/Ng Figure 4A and Supplemental Figure S4 ------- COMMENT: 17cceecf86230427 7 FQd4mK4OSZZi3AAzsR6Hd92s/Ng Figure 4A and Supplemental Figure S4 ------- COMMENT: 17cceecf86230427 8 FQd4mK4OSZZi3AAzsR6Hd92s/Ng Figure 4A and Supplemental Figure S4 ------- COMMENT: 17cceecf86230427 25 nUCcEDoowWdBc8w5z3A4wbzAAHc Figure 1F ------- COMMENT: 17cceecf86230427 26 1C09U3SGlHvPkvhPIrOlXx1qV8w Figure 2G ------- COMMENT: 17cceecf86230427 28 IbzTGJGTYrO5avcuWk9aLb2551M (Supplemental Figure S1A). ------- COMMENT: 17cceecf86230427 29 /YjgqxTXk0IGbMcXyFsAA8D/074 Figure 2E ------- COMMENT: 17cceecf86230427 31 +5DWSlcsOPQdiZW3/mMaq6bSgI8 fig3C ------- COMMENT: 17cceecf86230427 32 DhT34CozC8PTNcslNSOBEhxfvdI Figure S5D ------- COMMENT: 17cceecf86230427 33 DhT34CozC8PTNcslNSOBEhxfvdI Figure S5D ------- COMMENT: 17cceecf86230427 47 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: 17ce40c3c1fbe060 25 rBjTk9GdFKfqbl0cBbcg7IxJv28 (comment: inferred from the fact growth is impaired in the double mutant spc7-23/mad2 OR spc7-23/mph1 are growth impaired, so assumption is that spindle checkpoint is active in mutant) ------- COMMENT: 17f50a0cd9b7972b 89 l+f1R919mLn1hkTFl9hvZaQF59A fig 6 ------- COMMENT: 17f50a0cd9b7972b 90 l+f1R919mLn1hkTFl9hvZaQF59A fig 6 ------- COMMENT: 17f50a0cd9b7972b 91 l+f1R919mLn1hkTFl9hvZaQF59A fig 6 ------- COMMENT: 17f50a0cd9b7972b 92 l+f1R919mLn1hkTFl9hvZaQF59A fig 6 ------- COMMENT: 17f50a0cd9b7972b 93 l+f1R919mLn1hkTFl9hvZaQF59A fig 6 ------- COMMENT: 17f50a0cd9b7972b 94 l+f1R919mLn1hkTFl9hvZaQF59A fig 6 ------- COMMENT: 17f50a0cd9b7972b 95 l+f1R919mLn1hkTFl9hvZaQF59A fig 6 ------- COMMENT: 17f50a0cd9b7972b 96 l+f1R919mLn1hkTFl9hvZaQF59A fig 6 ------- COMMENT: 17f50a0cd9b7972b 97 l+f1R919mLn1hkTFl9hvZaQF59A fig 6 ------- COMMENT: 17f50a0cd9b7972b 98 l+f1R919mLn1hkTFl9hvZaQF59A fig 6 ------- COMMENT: 17f50a0cd9b7972b 99 l+f1R919mLn1hkTFl9hvZaQF59A fig 6 ------- COMMENT: 181d196fc3f2df51 1 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 181d196fc3f2df51 2 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 181d196fc3f2df51 3 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 181d196fc3f2df51 4 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 181d196fc3f2df51 5 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 181d196fc3f2df51 6 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 181d196fc3f2df51 7 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 181d196fc3f2df51 8 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 181d196fc3f2df51 9 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 181d196fc3f2df51 10 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 181d196fc3f2df51 11 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 181d196fc3f2df51 12 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 181d196fc3f2df51 13 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 181d196fc3f2df51 14 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 181d196fc3f2df51 15 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 181d196fc3f2df51 16 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 181d196fc3f2df51 17 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 181d196fc3f2df51 18 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 181d196fc3f2df51 19 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 181d196fc3f2df51 20 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 181d196fc3f2df51 21 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 181d196fc3f2df51 22 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 181d196fc3f2df51 23 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 181d196fc3f2df51 24 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 181d196fc3f2df51 25 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 181d196fc3f2df51 26 ZSoIdAAkba/kthzeLNn7KxqZ/Bw Fig. S6B ------- COMMENT: 181d196fc3f2df51 27 ZSoIdAAkba/kthzeLNn7KxqZ/Bw Fig. S6B ------- COMMENT: 181d196fc3f2df51 28 ZSoIdAAkba/kthzeLNn7KxqZ/Bw Fig. S6B ------- COMMENT: 181d196fc3f2df51 29 ZSoIdAAkba/kthzeLNn7KxqZ/Bw Fig. S6B ------- COMMENT: 181d196fc3f2df51 30 ZSoIdAAkba/kthzeLNn7KxqZ/Bw Fig. S6B ------- COMMENT: 181d196fc3f2df51 31 ZSoIdAAkba/kthzeLNn7KxqZ/Bw Fig. S6B ------- COMMENT: 181d196fc3f2df51 32 ZSoIdAAkba/kthzeLNn7KxqZ/Bw Fig. S6B ------- COMMENT: 181d196fc3f2df51 33 ZSoIdAAkba/kthzeLNn7KxqZ/Bw Fig. S6B ------- COMMENT: 181d196fc3f2df51 34 ZSoIdAAkba/kthzeLNn7KxqZ/Bw Fig. S6B ------- COMMENT: 181d196fc3f2df51 35 ZSoIdAAkba/kthzeLNn7KxqZ/Bw Fig. S6B ------- COMMENT: 181d196fc3f2df51 36 AoxCF6165PlYpVxgfR9odj3O31Y Fig. S6C ------- COMMENT: 181d196fc3f2df51 37 AoxCF6165PlYpVxgfR9odj3O31Y Fig. S6C ------- COMMENT: 181d196fc3f2df51 38 AoxCF6165PlYpVxgfR9odj3O31Y Fig. S6C ------- COMMENT: 181d196fc3f2df51 39 AoxCF6165PlYpVxgfR9odj3O31Y Fig. S6C ------- COMMENT: 181d196fc3f2df51 40 AoxCF6165PlYpVxgfR9odj3O31Y Fig. S6C ------- COMMENT: 181d196fc3f2df51 41 AoxCF6165PlYpVxgfR9odj3O31Y Fig. S6C ------- COMMENT: 181d196fc3f2df51 42 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: 181d196fc3f2df51 43 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: 181d196fc3f2df51 44 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: 181d196fc3f2df51 45 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: 181d196fc3f2df51 46 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: 181d196fc3f2df51 47 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: 181d196fc3f2df51 48 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: 181d196fc3f2df51 49 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: 181d196fc3f2df51 50 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: 181d196fc3f2df51 51 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: 181d196fc3f2df51 52 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: 181d196fc3f2df51 53 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: 181d196fc3f2df51 54 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: 181d196fc3f2df51 55 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: 181d196fc3f2df51 56 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: 181d196fc3f2df51 57 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: 181d196fc3f2df51 58 UYdl30f2oAnKjj3Tfq8Yt6o95Mg Fig. S7A ------- COMMENT: 181d196fc3f2df51 59 UYdl30f2oAnKjj3Tfq8Yt6o95Mg Fig. S7A ------- COMMENT: 181d196fc3f2df51 60 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 181d196fc3f2df51 61 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 181d196fc3f2df51 62 fDHS6UKTsqv3NAycdViAlo5BsOA Fig. S9B ------- COMMENT: 181d196fc3f2df51 63 fDHS6UKTsqv3NAycdViAlo5BsOA Fig. S9B ------- COMMENT: 181d196fc3f2df51 64 fDHS6UKTsqv3NAycdViAlo5BsOA Fig. S9B ------- COMMENT: 181d196fc3f2df51 65 fDHS6UKTsqv3NAycdViAlo5BsOA Fig. S9B ------- COMMENT: 1823b4aa025cae13 1 V7xQ/+rp7WpzWQdNhNiOoKAOtp8 (comment: requires Clp1 activity) ------- COMMENT: 1823b4aa025cae13 6 QJPotoK6XheD5l1kzvsbm5E85Ak ------- COMMENT: 1823b4aa025cae13 7 TreD69sSAyE3m9Mh3kMRUUK56Sg Fig 1 (comment: requires Clp1 activity) ------- COMMENT: 1823b4aa025cae13 8 zghCAOkbeCXKxcI3Sbw3/6ZJVp0 Fig 1 (comment: requires Klp9, Clp1 activity) ------- COMMENT: 1823b4aa025cae13 9 NZWnLqFNIZXOzwS7hcJnagOtt6k (comment: requires motor activity) ------- COMMENT: 1823b4aa025cae13 11 l+7e4y7iiLVRr7OE7Jip6RAQtA8 Fig 3 ------- COMMENT: 1823b4aa025cae13 12 4nUyWV9CHpjfZv8GwLKFYswDw9k (comment: Through Mad3 KEN20, does not require Mad2) ------- COMMENT: 1823b4aa025cae13 13 oXrahwH/EUTq3pU54FPDXMsc++Y Fig. 1 ------- COMMENT: 1823b4aa025cae13 14 KfrxVbBLk7c7bDCmHqxAUJjxGUw Figure 1B and S1B,C ------- COMMENT: 1823b4aa025cae13 15 KfrxVbBLk7c7bDCmHqxAUJjxGUw Figure 1B and S1B,C ------- COMMENT: 1823b4aa025cae13 16 KfrxVbBLk7c7bDCmHqxAUJjxGUw Figure 1B and S1B,C ------- COMMENT: 1823b4aa025cae13 17 KfrxVbBLk7c7bDCmHqxAUJjxGUw Figure 1B and S1B,C ------- COMMENT: 1823b4aa025cae13 18 KfrxVbBLk7c7bDCmHqxAUJjxGUw Figure 1B and S1B,C ------- COMMENT: 1823b4aa025cae13 19 KfrxVbBLk7c7bDCmHqxAUJjxGUw Figure 1B and S1B,C ------- COMMENT: 1823b4aa025cae13 20 KfrxVbBLk7c7bDCmHqxAUJjxGUw Figure 1B and S1B,C ------- COMMENT: 1823b4aa025cae13 21 KfrxVbBLk7c7bDCmHqxAUJjxGUw Figure 1B and S1B,C ------- COMMENT: 1823b4aa025cae13 22 KfrxVbBLk7c7bDCmHqxAUJjxGUw Figure 1B and S1B,C ------- COMMENT: 1823b4aa025cae13 23 KfrxVbBLk7c7bDCmHqxAUJjxGUw Figure 1B and S1B,C ------- COMMENT: 1823b4aa025cae13 24 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 1823b4aa025cae13 25 kRLT1/s8ectygqdBQv6nllzYjfA (Figures S1D and S1E) ------- COMMENT: 1823b4aa025cae13 26 Ak3XQKk6OSrmc8bEuTOr1Q9MNE0 (Figure 1D) ------- COMMENT: 1823b4aa025cae13 27 Ak3XQKk6OSrmc8bEuTOr1Q9MNE0 (Figure 1D) ------- COMMENT: 1823b4aa025cae13 28 KfrxVbBLk7c7bDCmHqxAUJjxGUw Figure 1B and S1B,C ------- COMMENT: 1823b4aa025cae13 29 KfrxVbBLk7c7bDCmHqxAUJjxGUw Figure 1B and S1B,C ------- COMMENT: 1823b4aa025cae13 30 aOqYL7F2KV5d9E4mVC2uoiGmluk Figure 1E ------- COMMENT: 1823b4aa025cae13 31 VJkDLT4c7F7XwY0hTsuwepWVCyA (Figures 1G and 1I; Figure S1F) ------- COMMENT: 1823b4aa025cae13 32 VJkDLT4c7F7XwY0hTsuwepWVCyA (Figures 1G and 1I; Figure S1F) ------- COMMENT: 1823b4aa025cae13 33 uh5g9oeqXc/OWAihnDGWAssGpB0 Figure 1H ------- COMMENT: 1823b4aa025cae13 34 dO46EeiHzSbG1ppnE+8rTE72OZM (Figures 1H) ------- COMMENT: 1823b4aa025cae13 35 dO46EeiHzSbG1ppnE+8rTE72OZM (Figures 1H) ------- COMMENT: 1823b4aa025cae13 36 OVBD7j1mDXMOhPbh3tHltwZzM6c Figure 1I ------- COMMENT: 1823b4aa025cae13 37 OVBD7j1mDXMOhPbh3tHltwZzM6c Figure 1I ------- COMMENT: 1823b4aa025cae13 38 /vqO1dGCLMFoS7oNUF4tlaMp6Vo (Figures 1J) ------- COMMENT: 1823b4aa025cae13 39 /vqO1dGCLMFoS7oNUF4tlaMp6Vo (Figures 1J) ------- COMMENT: 1823b4aa025cae13 40 /vqO1dGCLMFoS7oNUF4tlaMp6Vo (Figures 1J) ------- COMMENT: 1823b4aa025cae13 41 /vqO1dGCLMFoS7oNUF4tlaMp6Vo (Figures 1J) ------- COMMENT: 1823b4aa025cae13 42 /vqO1dGCLMFoS7oNUF4tlaMp6Vo (Figures 1J) ------- COMMENT: 1823b4aa025cae13 43 /vqO1dGCLMFoS7oNUF4tlaMp6Vo (Figures 1J) ------- COMMENT: 1823b4aa025cae13 44 /vqO1dGCLMFoS7oNUF4tlaMp6Vo (Figures 1J) ------- COMMENT: 1823b4aa025cae13 45 /vqO1dGCLMFoS7oNUF4tlaMp6Vo (Figures 1J) ------- COMMENT: 1823b4aa025cae13 46 /vqO1dGCLMFoS7oNUF4tlaMp6Vo (Figures 1J) ------- COMMENT: 1823b4aa025cae13 47 /vqO1dGCLMFoS7oNUF4tlaMp6Vo (Figures 1J) ------- COMMENT: 1823b4aa025cae13 48 Kr6GlcTPFCDDDrQeQSdT+ftOa3s (Figures S2A) ------- COMMENT: 1823b4aa025cae13 49 Kr6GlcTPFCDDDrQeQSdT+ftOa3s (Figures S2A) ------- COMMENT: 1823b4aa025cae13 50 B+BO5gH01QQtnnHkkw+/7+ajJTU (Figures S2B) ------- COMMENT: 1823b4aa025cae13 51 B+BO5gH01QQtnnHkkw+/7+ajJTU (Figures S2B) ------- COMMENT: 1823b4aa025cae13 55 Y/rK+VW4RXs7JXT+KWmX2SxPkAs Figure 2A ------- COMMENT: 1823b4aa025cae13 56 5kr7c6WBjJUrsnKNv/uMwzy6ito Figure 2A; Figure S3A ------- COMMENT: 1823b4aa025cae13 57 5kr7c6WBjJUrsnKNv/uMwzy6ito Figure 2A; Figure S3A ------- COMMENT: 1823b4aa025cae13 58 4O8xcHisKrNN/tgvTKDomttTtoU Figure S3B ------- COMMENT: 1823b4aa025cae13 59 4O8xcHisKrNN/tgvTKDomttTtoU Figure S3B ------- COMMENT: 1823b4aa025cae13 60 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: 1823b4aa025cae13 61 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: 1823b4aa025cae13 62 QW+3xXwltMCGNF9yylWu7Uoy124 Figure S3C/D ------- COMMENT: 1823b4aa025cae13 64 C9q+H4ghbn4lvQCiMc6bjw1KPOE (Figure S4C) ------- COMMENT: 1823b4aa025cae13 65 C9q+H4ghbn4lvQCiMc6bjw1KPOE (Figure S4C) ------- COMMENT: 1823b4aa025cae13 66 C9q+H4ghbn4lvQCiMc6bjw1KPOE (Figure S4C) ------- COMMENT: 1823b4aa025cae13 67 C9q+H4ghbn4lvQCiMc6bjw1KPOE (Figure S4C) ------- COMMENT: 1823b4aa025cae13 68 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: 1823b4aa025cae13 69 +t2qCCGlK6xrXs1Rvm4vTpyZddI Fig 4 A ------- COMMENT: 1823b4aa025cae13 70 +t2qCCGlK6xrXs1Rvm4vTpyZddI Fig 4 A ------- COMMENT: 1823b4aa025cae13 71 +t2qCCGlK6xrXs1Rvm4vTpyZddI Fig 4 A ------- COMMENT: 1823b4aa025cae13 72 +t2qCCGlK6xrXs1Rvm4vTpyZddI Fig 4 A ------- COMMENT: 1823b4aa025cae13 73 +t2qCCGlK6xrXs1Rvm4vTpyZddI Fig 4 A ------- COMMENT: 1823b4aa025cae13 74 +t2qCCGlK6xrXs1Rvm4vTpyZddI Fig 4 A ------- COMMENT: 1823b4aa025cae13 75 +t2qCCGlK6xrXs1Rvm4vTpyZddI Fig 4 A ------- COMMENT: 1823b4aa025cae13 76 eegRxKSZYvCfekqJ3GgWm1RsMSE Fig 4 D ------- COMMENT: 1823b4aa025cae13 77 eegRxKSZYvCfekqJ3GgWm1RsMSE Fig 4 D ------- COMMENT: 1823b4aa025cae13 78 eegRxKSZYvCfekqJ3GgWm1RsMSE Fig 4 D ------- COMMENT: 1823b4aa025cae13 79 wf185u4DRDkg7Sbpsn3gLe6+2NY Figure 4E ------- COMMENT: 1823b4aa025cae13 80 eegRxKSZYvCfekqJ3GgWm1RsMSE Fig 4 D ------- COMMENT: 1823b4aa025cae13 81 wf185u4DRDkg7Sbpsn3gLe6+2NY Figure 4E ------- COMMENT: 1823b4aa025cae13 82 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: 1823b4aa025cae13 83 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: 1823b4aa025cae13 84 RIutbkMWmPaNTG97zq8Wh9lBF14 (Figure 5B) ------- COMMENT: 1823b4aa025cae13 85 RIutbkMWmPaNTG97zq8Wh9lBF14 (Figure 5B) ------- COMMENT: 1823b4aa025cae13 87 +t2qCCGlK6xrXs1Rvm4vTpyZddI Fig 4A ------- COMMENT: 1823b4aa025cae13 88 +t2qCCGlK6xrXs1Rvm4vTpyZddI Fig 4A ------- COMMENT: 1823b4aa025cae13 89 +t2qCCGlK6xrXs1Rvm4vTpyZddI Fig 4A ------- COMMENT: 1823b4aa025cae13 90 +t2qCCGlK6xrXs1Rvm4vTpyZddI Fig 4A ------- COMMENT: 1823b4aa025cae13 91 eegRxKSZYvCfekqJ3GgWm1RsMSE Fig 4D ------- COMMENT: 1823b4aa025cae13 92 eegRxKSZYvCfekqJ3GgWm1RsMSE Fig 4D ------- COMMENT: 1823b4aa025cae13 93 eegRxKSZYvCfekqJ3GgWm1RsMSE Fig 4D ------- COMMENT: 1823b4aa025cae13 94 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: 1823b4aa025cae13 95 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: 1854edb1fb4a0e10 1 vCQON3u45tbsiRVXyP6m4OM3Ct0 (comment: 22.3%) ------- COMMENT: 1854edb1fb4a0e10 2 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: 1854edb1fb4a0e10 4 EK3cJaIpApHaZfDj/il8fbVV8BY fig S2 ------- COMMENT: 1854edb1fb4a0e10 5 Krt8IEN2thpV+MLPwLEVMzonARU fig 2B ------- COMMENT: 1854edb1fb4a0e10 6 VECXT0OCE3UdwQfSD1EJ2ckMGOo fig 2AB ------- COMMENT: 1854edb1fb4a0e10 7 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig 2 ------- COMMENT: 1854edb1fb4a0e10 8 Xb1L1D0oeozHWjRnmVjhdBSDo98 Fig. S3A ------- COMMENT: 1854edb1fb4a0e10 9 2SkyLksWV7akLwZiR4xvvnTu/2Q Fig. ------- COMMENT: 1854edb1fb4a0e10 10 wZnc3Hrw3ShCsLNlIKKR9jS2o1E Fig. S1B-j ------- COMMENT: 1854edb1fb4a0e10 11 RPdkqAedukJ0cWGJswoHwJ+rPAE Fig. 3 ------- COMMENT: 1854edb1fb4a0e10 15 RPdkqAedukJ0cWGJswoHwJ+rPAE Fig. 3 ------- COMMENT: 1854edb1fb4a0e10 16 GzQQRIirPoWD2NK+XSQSE9jSc3U ------- COMMENT: 1854edb1fb4a0e10 17 GzQQRIirPoWD2NK+XSQSE9jSc3U ------- COMMENT: 1854edb1fb4a0e10 18 GzQQRIirPoWD2NK+XSQSE9jSc3U ------- COMMENT: 1854edb1fb4a0e10 19 GzQQRIirPoWD2NK+XSQSE9jSc3U ------- COMMENT: 1854edb1fb4a0e10 20 trtqYqH5Ul59/V1W5edXsV0UNPE (comment: increased affinity) ------- COMMENT: 1854edb1fb4a0e10 21 LobEWKli0wGZTfWu/275pRoFi/I Fig. 7F ------- COMMENT: 1854edb1fb4a0e10 22 LobEWKli0wGZTfWu/275pRoFi/I Fig. 7F ------- COMMENT: 186e8e06649ff2d7 5 eSk1u8Pw52h+cndmf4H7BNlGIOA ------- COMMENT: 186e8e06649ff2d7 6 3KbXupf3iJoMU74sXu7nAbDYcNo ------- COMMENT: 186e8e06649ff2d7 7 nPAxpb2HVvv2jbYvE7/Bzu6rYAY (comment: 26% slower) ------- COMMENT: 186e8e06649ff2d7 8 CL0r7JsymN+WuJmJptVVl0coEys (comment: 23% slower) ------- COMMENT: 186e8e06649ff2d7 10 NLE3U99o0h58vtjZtPpcTYutL5o ------- COMMENT: 186e8e06649ff2d7 11 NLE3U99o0h58vtjZtPpcTYutL5o ------- COMMENT: 186e8e06649ff2d7 12 1ytXH7nlEONCLUjR7pFzoALDAFw ------- COMMENT: 186e8e06649ff2d7 13 1ytXH7nlEONCLUjR7pFzoALDAFw ------- COMMENT: 186e8e06649ff2d7 14 5RHIrbWzNWHM3pfIr4VYUYEUTME (comment: residues 20â€-40 in synthetic peptide/ dissociation constant of 1.1 nM) ------- COMMENT: 186e8e06649ff2d7 24 ow5S1FSkgXMM3YPygt8S6MjPQzw (comment: smears as does not self associate, but localizes to medial cortex) ------- COMMENT: 186e8e06649ff2d7 30 NLE3U99o0h58vtjZtPpcTYutL5o ------- COMMENT: 186e8e06649ff2d7 31 NLE3U99o0h58vtjZtPpcTYutL5o ------- COMMENT: 186e8e06649ff2d7 33 5RHIrbWzNWHM3pfIr4VYUYEUTME (comment: residues 20â€-40 in synthetic peptide/ dissociation constant of 1.1 nM) ------- COMMENT: 18bc2c41ccf91f5a 1 KXJeQeLvd36n52aBczWLIxoLHn4 (comment: UAAU motif) ------- COMMENT: 18cbd65c4f603fdb 2 NjjlRfWqENBYuwQjxEqcgtvNqIg fig. 1 ------- COMMENT: 18cbd65c4f603fdb 3 NjjlRfWqENBYuwQjxEqcgtvNqIg fig. 1 ------- COMMENT: 18cbd65c4f603fdb 4 QX8OmX5bpOWrOtQxQiElzkLmz6c Supplementary Fig. S2a ------- COMMENT: 18cbd65c4f603fdb 5 QX8OmX5bpOWrOtQxQiElzkLmz6c Supplementary Fig. S2a ------- COMMENT: 18cbd65c4f603fdb 6 QX8OmX5bpOWrOtQxQiElzkLmz6c Supplementary Fig. S2a ------- COMMENT: 18cbd65c4f603fdb 7 QX8OmX5bpOWrOtQxQiElzkLmz6c Supplementary Fig. S2a ------- COMMENT: 18cbd65c4f603fdb 8 NjjlRfWqENBYuwQjxEqcgtvNqIg fig.1 ------- COMMENT: 18cbd65c4f603fdb 9 Khp4emiB2X0MPc8gyDi+Qsr6ONo Fig. 2a ------- COMMENT: 18cbd65c4f603fdb 10 Khp4emiB2X0MPc8gyDi+Qsr6ONo Fig. 2a ------- COMMENT: 18cbd65c4f603fdb 11 Khp4emiB2X0MPc8gyDi+Qsr6ONo Fig. 2a ------- COMMENT: 18cbd65c4f603fdb 12 Khp4emiB2X0MPc8gyDi+Qsr6ONo Fig. 2a ------- COMMENT: 18cbd65c4f603fdb 13 x/NZEmVqdHSTslB9O4JG1xKSqyY Fig. 2b ------- COMMENT: 18cbd65c4f603fdb 14 x/NZEmVqdHSTslB9O4JG1xKSqyY Fig. 2b ------- COMMENT: 18cbd65c4f603fdb 15 x/NZEmVqdHSTslB9O4JG1xKSqyY Fig. 2b ------- COMMENT: 18cbd65c4f603fdb 16 x/NZEmVqdHSTslB9O4JG1xKSqyY Fig. 2b ------- COMMENT: 18cbd65c4f603fdb 17 x/NZEmVqdHSTslB9O4JG1xKSqyY Fig. 2b ------- COMMENT: 18cbd65c4f603fdb 18 x/NZEmVqdHSTslB9O4JG1xKSqyY Fig. 2b ------- COMMENT: 18cbd65c4f603fdb 19 x/NZEmVqdHSTslB9O4JG1xKSqyY Fig. 2b ------- COMMENT: 18cbd65c4f603fdb 20 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 18cbd65c4f603fdb 21 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 18cbd65c4f603fdb 22 hj6iDyxAZeWglE5qndTQgSgxBhw Fig. 4b ------- COMMENT: 18cbd65c4f603fdb 23 hj6iDyxAZeWglE5qndTQgSgxBhw Fig. 4b ------- COMMENT: 18cbd65c4f603fdb 24 hj6iDyxAZeWglE5qndTQgSgxBhw Fig. 4b ------- COMMENT: 18cbd65c4f603fdb 25 hj6iDyxAZeWglE5qndTQgSgxBhw Fig. 4b ------- COMMENT: 18cbd65c4f603fdb 26 x/NZEmVqdHSTslB9O4JG1xKSqyY Fig. 2b ------- COMMENT: 18cbd65c4f603fdb 27 QX8OmX5bpOWrOtQxQiElzkLmz6c Supplementary Fig. S2a ------- COMMENT: 18cbd65c4f603fdb 30 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 18cbd65c4f603fdb 35 1hTNXRMhDUUO8tXQzE+2Go2g3yk (comment: this is an inference, but almost certainly true based on the genetics) ------- COMMENT: 18cbd65c4f603fdb 36 npslThVTxAtUhDLIqN/FYk5vNSg (comment: Notably, we detected only a single combination of PP2A subunits associated with SpSgo1, namely SpPaa1A–SpPar1B′–SpPpa2C) ------- COMMENT: 1900a00e16aa4080 1 aaGlWocotAuVcdec+bsQce+I4kY fig. 3A ------- COMMENT: 1900a00e16aa4080 2 aaGlWocotAuVcdec+bsQce+I4kY fig. 3A ------- COMMENT: 1900a00e16aa4080 3 aaGlWocotAuVcdec+bsQce+I4kY fig. 3A ------- COMMENT: 1900a00e16aa4080 4 aaGlWocotAuVcdec+bsQce+I4kY fig. 3A ------- COMMENT: 1900a00e16aa4080 5 aaGlWocotAuVcdec+bsQce+I4kY fig. 3A ------- COMMENT: 1900a00e16aa4080 6 aaGlWocotAuVcdec+bsQce+I4kY fig. 3A ------- COMMENT: 1900a00e16aa4080 7 aaGlWocotAuVcdec+bsQce+I4kY fig. 3A ------- COMMENT: 1900a00e16aa4080 8 1tlY+AuqilukiW8g6CwM2H52AXw (comment: caspase) ------- COMMENT: 1900a00e16aa4080 9 1tlY+AuqilukiW8g6CwM2H52AXw (comment: caspase) ------- COMMENT: 1900a00e16aa4080 10 1tlY+AuqilukiW8g6CwM2H52AXw (comment: caspase) ------- COMMENT: 190f5f719398b3d9 1 zGY1QoY/OCsC9NEFv9JqYUpkn+k (comment: changed from: heterochromatin organization involved in chromatin silencing) ------- COMMENT: 190f5f719398b3d9 2 RALdV5r7BhvDDbTt+je/1Dr+PQA Supp 1b ------- COMMENT: 190f5f719398b3d9 4 RALdV5r7BhvDDbTt+je/1Dr+PQA Supp 1b ------- COMMENT: 190f5f719398b3d9 5 RALdV5r7BhvDDbTt+je/1Dr+PQA Supp 1b ------- COMMENT: 190f5f719398b3d9 6 RALdV5r7BhvDDbTt+je/1Dr+PQA Supp 1b ------- COMMENT: 190f5f719398b3d9 7 ZQQ3E4cVfQlorUcfZjVsZKgdFw0 (comment: EV3) ------- COMMENT: 190f5f719398b3d9 8 ZQQ3E4cVfQlorUcfZjVsZKgdFw0 (comment: EV3) ------- COMMENT: 190f5f719398b3d9 9 ZQQ3E4cVfQlorUcfZjVsZKgdFw0 (comment: EV3) ------- COMMENT: 190f5f719398b3d9 10 glJW1aOyj4jhjS/C9sk9B+zU/dU Figure 4a ------- COMMENT: 190f5f719398b3d9 11 glJW1aOyj4jhjS/C9sk9B+zU/dU Figure 4a ------- COMMENT: 190f5f719398b3d9 12 glJW1aOyj4jhjS/C9sk9B+zU/dU Figure 4a ------- COMMENT: 190f5f719398b3d9 13 ZQQ3E4cVfQlorUcfZjVsZKgdFw0 (comment: EV3) ------- COMMENT: 190f5f719398b3d9 14 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig. 5 ------- COMMENT: 190f5f719398b3d9 15 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig. 5 ------- COMMENT: 190f5f719398b3d9 16 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig. 5 ------- COMMENT: 190f5f719398b3d9 17 l+f1R919mLn1hkTFl9hvZaQF59A fig. 6 ------- COMMENT: 190f5f719398b3d9 18 l+f1R919mLn1hkTFl9hvZaQF59A fig. 6 ------- COMMENT: 190f5f719398b3d9 19 ZQQ3E4cVfQlorUcfZjVsZKgdFw0 (comment: EV3) ------- COMMENT: 190f5f719398b3d9 20 ZQQ3E4cVfQlorUcfZjVsZKgdFw0 (comment: EV3) ------- COMMENT: 190f5f719398b3d9 21 zGY1QoY/OCsC9NEFv9JqYUpkn+k (comment: changed from: heterochromatin organization involved in chromatin silencing) ------- COMMENT: 1912f002dd642ee1 39 JpQzZ0LpQ9wfpfyYrUb0md21DZk (comment: indicates Hsf1 activation) ------- COMMENT: 191643d2928456ea 26 8O0T+WHFXWFlxAxsYbs4qEhDKVM (comment: assayed in vitro using casein) ------- COMMENT: 191643d2928456ea 28 ae9cw1k0x8uaGxFqe2b1gFv/By0 (comment: grows in three dimensions instead of just at cell ends) ------- COMMENT: 191643d2928456ea 48 qq3zoXW7oQuBNIHqfthlt0i3ZOA (comment: same as cdc25-22 alone) ------- COMMENT: 191643d2928456ea 49 W6ABl9xDpkeHDhSQSa00SyZKzZw (comment: same as cdc2-33 alone) ------- COMMENT: 191a34a7eb84c981 1 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 191a34a7eb84c981 2 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 199abb8a7b85407c 12 0Ignyum22RxvN1nFczB37cs8CPg ------- COMMENT: 199abb8a7b85407c 14 bCRjh3JpId5Z0uB82HCWHakEkVI (comment: decreased cell pop is not a child of this term) ------- COMMENT: 199abb8a7b85407c 17 OO/oWvRJ3iKA5cU4jchi1ZYPeMg ------- COMMENT: 199abb8a7b85407c 25 Mu6EAPuf7ZQaTe5aN857ZZ384Ys ------- COMMENT: 199abb8a7b85407c 64 NKbVEw5untp77BVAqkTHmm4bESI ------- COMMENT: 19cb5710ede28157 1 8RpTQ8l8TR6OOQ2a0mLPYhlc6r0 (comment: localization requires F-actin -assayed using latrunculin A and membrane rafts -assayed using filipin) ------- COMMENT: 1a58c3ee1e7be06d 2 gnkqlArrnbou6qRsUNCHVL9JTPw ------- COMMENT: 1a58c3ee1e7be06d 6 HiQhWitXHn1M7NVuXHIYFSRUSLk (comment: required for wildtype rates of actin cable retrograde flow in myo52∆ cells) ------- COMMENT: 1a738af2bae8f80d 1 guG86kimGVXiKimpCjMA2AR9aX4 (comment: cerevisiae substrate) ------- COMMENT: 1a9429aa838169ed 78 IP4i7+pqkl0K+lJqcpCUKeTc89c (comment: present throughout mitotic cell cycle) ------- COMMENT: 1ac71137f0c65839 18 uWqkt6RPjTG2/4Lo6LcqUS+lYpE fig 1A (comment: they say it is dramatically reduced....between med/low severity... "dramatically reduced on glycerol medium, which requires high mitochondrial respiratory activity at 30 °C") ------- COMMENT: 1ac71137f0c65839 20 B0ukIngpriuRc0Mw6s/M4vu6VOQ fig2 A The Dmti2 mutant was not able to grow at all on medium containing glycerol at the restrictive temperature of 37 °C ------- COMMENT: 1ac71137f0c65839 21 wb8+3kAgYc7JGuHj/SRKrzp4b3M ------- COMMENT: 1ac71137f0c65839 23 oXrahwH/EUTq3pU54FPDXMsc++Y fig. 1 ------- COMMENT: 1ac71137f0c65839 25 oXrahwH/EUTq3pU54FPDXMsc++Y fig. 1 ------- COMMENT: 1ac71137f0c65839 26 oXrahwH/EUTq3pU54FPDXMsc++Y fig. 1 ------- COMMENT: 1ac71137f0c65839 27 oXrahwH/EUTq3pU54FPDXMsc++Y fig. 1 ------- COMMENT: 1ac71137f0c65839 28 oXrahwH/EUTq3pU54FPDXMsc++Y fig. 1 ------- COMMENT: 1ac71137f0c65839 29 oXrahwH/EUTq3pU54FPDXMsc++Y fig. 1 ------- COMMENT: 1ac71137f0c65839 31 oXrahwH/EUTq3pU54FPDXMsc++Y fig. 1 ------- COMMENT: 1ac71137f0c65839 44 KabKAyY7rfvUnMgQyaZ+eyXl8rY fig 1A ------- COMMENT: 1ac71137f0c65839 45 KabKAyY7rfvUnMgQyaZ+eyXl8rY fig 1A ------- COMMENT: 1ac71137f0c65839 46 KabKAyY7rfvUnMgQyaZ+eyXl8rY fig 1A ------- COMMENT: 1ac71137f0c65839 47 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 1ac71137f0c65839 48 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 1ac71137f0c65839 49 rFlDDXFKTzEqmT7kq8UpdaKuxjg fig. 4B ------- COMMENT: 1ac71137f0c65839 50 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig 5 ------- COMMENT: 1ac71137f0c65839 51 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig 5 ------- COMMENT: 1ac71137f0c65839 52 l+f1R919mLn1hkTFl9hvZaQF59A fig 6 ------- COMMENT: 1ad0c6a096c036ff 10 AY5x6UAoQx+iC/aRYan3qcb4x8Q ------- COMMENT: 1ad0c6a096c036ff 12 AY5x6UAoQx+iC/aRYan3qcb4x8Q ------- COMMENT: 1ae670cde3faca1e 4 lD5PutZwYQB8fKF4w4B2F5aX6L8 (comment: polysomal profiling) ------- COMMENT: 1ae670cde3faca1e 5 lD5PutZwYQB8fKF4w4B2F5aX6L8 (comment: polysomal profiling) ------- COMMENT: 1af71a20816f7f92 1 Z4anPSNYMEu3wigLERRwE7f7HRE fig 1. Chronic exposure to analogue through growth on solid medium reiterated the acute impact of analogue inhibition in liquid culture (Fig. 1A) and established that plo1.as8 is most effectively inhibited by 3BrB- PP1 (Fig. 1B). ------- COMMENT: 1af71a20816f7f92 3 dzzuS8ixBhRKdTQswgAs6EE6T1k Fig. 2B orb5.as2 displayed moderate sensitivity to 30 mM of 3BrB-PP1, whereas orb5.as1 showed none ------- COMMENT: 1af71a20816f7f92 5 kyI1I62qOvlhaNwm+5TrmmMHHmA Fig 1. Chronic exposure to analogue through growth on solid medium reiterated the acute impact of analogue inhibition in liquid culture (Fig. 1A) and established that plo1.as8 is most effectively inhibited by 3BrB- PP1 (Fig. 1B). ------- COMMENT: 1af71a20816f7f92 6 WSIMkgM05EWOCTmIA1bI9/tqMsw Fig 1. ------- COMMENT: 1af71a20816f7f92 7 WSIMkgM05EWOCTmIA1bI9/tqMsw Fig 1. ------- COMMENT: 1af71a20816f7f92 8 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 1af71a20816f7f92 9 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: 1af71a20816f7f92 10 +DWBaS4Owsehr23gt4vhlUqQ/ls (Fig. 2B). The M167F mutation in either the orb5.as1 or orb5.as2 backbone generated the orb5.as8 and orb5.as9 alleles, which were more sensitive to analogue inhibition than the respective parental allele ------- COMMENT: 1af71a20816f7f92 11 dzzuS8ixBhRKdTQswgAs6EE6T1k Fig. 2B orb5.as2 displayed moderate sensitivity to 30 mM of 3BrB-PP1, whereas orb5.as1 showed none ------- COMMENT: 1af71a20816f7f92 12 dzzuS8ixBhRKdTQswgAs6EE6T1k Fig. 2B orb5.as2 displayed moderate sensitivity to 30 mM of 3BrB-PP1, whereas orb5.as1 showed none ------- COMMENT: 1af71a20816f7f92 13 dzzuS8ixBhRKdTQswgAs6EE6T1k Fig. 2B orb5.as2 displayed moderate sensitivity to 30 mM of 3BrB-PP1, whereas orb5.as1 showed none ------- COMMENT: 1af71a20816f7f92 14 +DWBaS4Owsehr23gt4vhlUqQ/ls (Fig. 2B). The M167F mutation in either the orb5.as1 or orb5.as2 backbone generated the orb5.as8 and orb5.as9 alleles, which were more sensitive to analogue inhibition than the respective parental allele ------- COMMENT: 1af71a20816f7f92 16 kBxv/OhMDCREjapamUyUzljGqaY rb5.as8 inhibition did not produce the ‘orb’ phenotype observed in the original orb5.ts mutants at the restrictive temperature (data not shown) ------- COMMENT: 1af71a20816f7f92 17 4GS4sHBEMu9a7G+HP5lgg1MOG8A (Table 1; Fig. 3A) ------- COMMENT: 1af71a20816f7f92 18 4GS4sHBEMu9a7G+HP5lgg1MOG8A (Table 1; Fig. 3A) ------- COMMENT: 1af71a20816f7f92 19 23A7gKYH++mwxpDaLPxFOcAYx6Y fig 3c ------- COMMENT: 1af71a20816f7f92 20 VCJnuHNCfGhNC8UdZBb1fg6HZwc fig 3c. We therefore compared the ability of wee1.as1 and wee1.as8 to suppress cdc25.22 lethality at 36 ̊C. Inhibition of Wee1.as8 but not Wee1.as1 activity with analogue addition suppressed cdc25.22 lethality at 36 ̊C (Fig. 3B). A comparison of four ATP analogues revealed that the suppression (and therefore Wee1 inhibition) was most effective with 3BrB-PP1 (Fig. 3C). ------- COMMENT: 1af71a20816f7f92 21 PPJPuBHc7q/4/FcHlrERMK6d4Vc fig 4c. ------- COMMENT: 1af71a20816f7f92 22 q7yQ4AbC2bEj6KiCpphVWV06cCo dns ------- COMMENT: 1af71a20816f7f92 23 Sio/n4WkIHXXyoDMh7Sm4WSHBkE Analogue-released wee1.as8 cdc25.22 cut9.665 cells transiently accumulated much higher levels of metaphase spindles than wild-type cells before they ‘leaked’ through this mitotic arrest to execute telophase and cytokinesis with the classic ‘cut’ phenotype that originally led to the identification of the cut9.665 mutation (Fig. 6A,B) ------- COMMENT: 1af71a20816f7f92 24 tzjCtrEDKtX+rk9sV0VCbk1GUlk as arrested at 36 ̊C for 4.25 hours and released into synchronous mitosis by Wee1 inhibition using 30 mM 3BrB-PP1. The figure shows tubulin immunofluorescence and DAPI signals of cells 3 hours after release to reveal the characteristic ‘crows foot’ configuration of microtubules of cut7 mutants as the two halves of the mitotic spindle fail to interdigitate. ------- COMMENT: 1af71a20816f7f92 25 4xTAzSZW6rq3FqsTXPCX+2dMJtA The static FACS profiles established that replication was indeed inhibited in analogue-released cdc10.v50 wee1.as8 cdc25.22 cells after analogue addition (Fig. 7B, right panel). ------- COMMENT: 1b1b8ae732261385 12 HKSB0InU5HwslfYUoBZiq7uZriY (comment: NADP-GDH-defective) ------- COMMENT: 1b2540a523d29b62 4 EnHFicD3lY83paem3ehZ6Bk4hoI (comment: transient phenotype, they then attempt to divide without segregation) ------- COMMENT: 1b2540a523d29b62 5 EnHFicD3lY83paem3ehZ6Bk4hoI (comment: transient phenotype, they then attempt to divide without segregation) ------- COMMENT: 1b2540a523d29b62 11 yA0ozHHlNdWgg825bHWjg8OzJiY (comment: they show it is not abnormal regulation of rereplication in cdc25 double mutant expts) ------- COMMENT: 1b2540a523d29b62 19 EnHFicD3lY83paem3ehZ6Bk4hoI (comment: transient phenotype, they then attempt to divide without segregation) ------- COMMENT: 1b2540a523d29b62 20 EnHFicD3lY83paem3ehZ6Bk4hoI (comment: transient phenotype, they then attempt to divide without segregation) ------- COMMENT: 1b2540a523d29b62 21 yA0ozHHlNdWgg825bHWjg8OzJiY (comment: they show it is not abnormal regulation of rereplication in cdc25 double mutant expts) ------- COMMENT: 1b4431af072117e2 5 qR/eYzDFhhPlMD7yWZWVJVzVPbo Figure 2B ------- COMMENT: 1b4431af072117e2 6 qR/eYzDFhhPlMD7yWZWVJVzVPbo Figure 2B ------- COMMENT: 1b4431af072117e2 7 JJ61Rr+BzZ+yYkS4ndaMB26Ouxc Figure 2B ------- COMMENT: 1b4431af072117e2 8 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 1b4431af072117e2 9 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 1b4431af072117e2 10 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 1b4431af072117e2 11 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 1b4431af072117e2 12 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 1b4431af072117e2 13 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 1b4431af072117e2 14 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 1b4431af072117e2 15 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 1b4431af072117e2 16 9hlIOfRLPcEcnquyYlDz4lkW/m0 fig 5 Consistent with previous immunolocalization studies (Gaits et al., 1998), wild-type Wis1-GFP showed solely cytoplasmic localization and little GFP signal was seen in the nuclear region (Figure 5A). ------- COMMENT: 1b4431af072117e2 17 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 1b4431af072117e2 18 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 1b4431af072117e2 19 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 1b4431af072117e2 21 6WHrd99GrMyMfIuyCQfHIajLgsk (comment: small amount) ------- COMMENT: 1b4431af072117e2 23 3eevvWaF2KGoQ0OIzOrKEK3xWho fig6 ------- COMMENT: 1b4431af072117e2 24 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: 1b4431af072117e2 25 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: 1b9146dba1a740a5 30 mXGU5xbxiFFcZ/mV4aQZLHWcs8o (comment: 2 sub populations spindle elongation delayed during anaphase. A spindle elongation delayed during anaphase B) ------- COMMENT: 1b9146dba1a740a5 49 VBo8xI9iOjrob9IpVJAijx8FXUk ------- COMMENT: 1b9692f34c4ac74a 29 KlHzhMSJA6aXwjq0iXtiJAbgrQc (comment: based on phenotype this annotation is possible) ------- COMMENT: 1bc3fe03ff243df2 2 PB3+PlzBmXMq5Ix4spbUUsTY2Wg The wtf4poison proteins is distributed throughout asci and spores in the absence of the wtf4antidote. The antidote assembles with the poison and then both proteins are localized to the vacuole in spores. ------- COMMENT: 1bc92c0f892db627 3 /uvClJG+PzjVHkDWgslzb4fkZmE (comment: This is in presence of 30µM Cutin-1 for 6 hours. Wild type cells show 36.3% abnormal chromosome segregation in same conditions) ------- COMMENT: 1bc92c0f892db627 5 WZHEfv2b9inZgyPES3rOrhWOcUI (comment: CONDITION Growth was assayed in presence of 10µM Cutin-1.) ------- COMMENT: 1bc92c0f892db627 6 NxWX959AX0DLn07Q20+dFnLPmvU shows no chromosome defects or cell length defects growth in presence of 10-100µM Cutin-1 for 15 hours at 100µM Cutin-1 reduced to 70% compared to ~10% in wild type ------- COMMENT: 1bc92c0f892db627 8 aJQGeOUUtbIgV6c8qwaexD/KoSI (comment: CONDITION +10µM Cutin-1) ------- COMMENT: 1bc92c0f892db627 9 eUu3FFaAKeCuSkn0nuIxPreu1lo growth in presence of 10-100µM Cutin-1 for 15 hours at 100µM Cutin-1 reduced to 70% compared to ~10% in wild type; assayed in presence of 30µM Cutin-1 for 6 hours shows no chromosome defects or cell length defects ------- COMMENT: 1bc92c0f892db627 11 /uvClJG+PzjVHkDWgslzb4fkZmE (comment: This is in presence of 30µM Cutin-1 for 6 hours. Wild type cells show 36.3% abnormal chromosome segregation in same conditions) ------- COMMENT: 1bc92c0f892db627 13 y77N7ylMmfRiiA3uet23R5u46sw (comment: CONDITION cells grown at 29°C for 6 hours in 30µM Cutin-1) ------- COMMENT: 1bc92c0f892db627 14 3KH+Ztdjt+JR6DAt41KrscSzx00 (comment: The size of the nucleus is not actually abnormal, it is the right size for the cell size but is variable because of the variable cell size at division) ------- COMMENT: 1bc92c0f892db627 15 LH/gNBqfrzSaHanyaYQh7qhwXkg (comment: the resistance to Cutin-1 is dependent on nuclear size. Longer cells have a larger nucleus and are more resistant compared to smaller cells with a smaller nucleus) ------- COMMENT: 1bc92c0f892db627 18 2z86XyHlJaeU/DR0+wLQRYsItOE Cells show increased mitotic chromosome segregation defects in presence of Cutin-1 ------- COMMENT: 1bc92c0f892db627 19 y77N7ylMmfRiiA3uet23R5u46sw (comment: CONDITION cells grown at 29°C for 6 hours in 30µM Cutin-1) ------- COMMENT: 1bc92c0f892db627 20 y77N7ylMmfRiiA3uet23R5u46sw (comment: CONDITION cells grown at 29°C for 6 hours in 30µM Cutin-1) ------- COMMENT: 1bc92c0f892db627 21 y77N7ylMmfRiiA3uet23R5u46sw (comment: CONDITION cells grown at 29°C for 6 hours in 30µM Cutin-1) ------- COMMENT: 1bc92c0f892db627 22 y77N7ylMmfRiiA3uet23R5u46sw (comment: CONDITION cells grown at 29°C for 6 hours in 30µM Cutin-1) ------- COMMENT: 1bc92c0f892db627 23 o7WaHhDvnVqSwKkw5FXDhBFSuhw (comment: CONDITION cells grown at 29°C) ------- COMMENT: 1bc92c0f892db627 24 tNpCi/JxlyJ+RXXHyy5GygDtLic (comment: CONDITION Cells grown at 29°C) ------- COMMENT: 1bc92c0f892db627 25 y77N7ylMmfRiiA3uet23R5u46sw (comment: CONDITION cells grown at 29°C for 6 hours in 30µM Cutin-1) ------- COMMENT: 1bc92c0f892db627 26 y77N7ylMmfRiiA3uet23R5u46sw (comment: CONDITION cells grown at 29°C for 6 hours in 30µM Cutin-1) ------- COMMENT: 1bc92c0f892db627 27 SkhsgWOd1ZW22Rx9S9zkw7uW21k (comment: Cells show partial resistance to 30µM Cutin-1 for 6 hours.) ------- COMMENT: 1bdb389b19f988cd 1 /iAIBZaQjC4IhDNHX1xX3lbW4Q0 (comment: present with ammonium, allantoin, or proline nitrogen source) ------- COMMENT: 1bde96e4328928bf 43 Yme4jMnO7HjZyedL507zrpGy8mQ We propose that Mei2 turns off the DSR-Mmi1 system by sequestering Mmi1 to the dot and thereby secures stable expression of meiosis-specific transcripts (Abstract). ------- COMMENT: 1beda591c5c5e770 1 eCMi+2+M79gobfQ4X9J4EIrKsGA Fig 1 a ------- COMMENT: 1beda591c5c5e770 2 eCMi+2+M79gobfQ4X9J4EIrKsGA Fig 1 a ------- COMMENT: 1beda591c5c5e770 3 eCMi+2+M79gobfQ4X9J4EIrKsGA Fig 1 a ------- COMMENT: 1beda591c5c5e770 4 QGsIrdKYh0PwBVE+nvrH2Enokkg figure 1 a ------- COMMENT: 1beda591c5c5e770 5 ax8WDdnw6R5oMg+EqSgmHUfXkMo figure 1 b (comment: is described as a pear, but is cylindrical short and wide....) ------- COMMENT: 1beda591c5c5e770 6 QGsIrdKYh0PwBVE+nvrH2Enokkg figure 1 a ------- COMMENT: 1beda591c5c5e770 7 QGsIrdKYh0PwBVE+nvrH2Enokkg figure 1 a ------- COMMENT: 1beda591c5c5e770 8 i+pZZ1O9k+libAHisFcXjZ0t6y8 figure 1 E ------- COMMENT: 1beda591c5c5e770 9 i+pZZ1O9k+libAHisFcXjZ0t6y8 figure 1 E ------- COMMENT: 1beda591c5c5e770 10 i+pZZ1O9k+libAHisFcXjZ0t6y8 figure 1 E ------- COMMENT: 1beda591c5c5e770 11 i+pZZ1O9k+libAHisFcXjZ0t6y8 figure 1 E ------- COMMENT: 1beda591c5c5e770 12 3kxCyWx4WkFMHAy62UrV4hjkGZw figure 1 F ------- COMMENT: 1beda591c5c5e770 13 3kxCyWx4WkFMHAy62UrV4hjkGZw figure 1 F ------- COMMENT: 1beda591c5c5e770 17 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 1beda591c5c5e770 18 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 1beda591c5c5e770 19 vypE/9RkJ/g9EJO+RWnvoFzccKo fig 3a ------- COMMENT: 1beda591c5c5e770 21 vypE/9RkJ/g9EJO+RWnvoFzccKo fig 3a ------- COMMENT: 1beda591c5c5e770 22 vypE/9RkJ/g9EJO+RWnvoFzccKo fig 3a ------- COMMENT: 1beda591c5c5e770 23 vypE/9RkJ/g9EJO+RWnvoFzccKo fig 3a ------- COMMENT: 1beda591c5c5e770 24 vypE/9RkJ/g9EJO+RWnvoFzccKo fig 3a ------- COMMENT: 1beda591c5c5e770 25 vypE/9RkJ/g9EJO+RWnvoFzccKo fig 3a ------- COMMENT: 1beda591c5c5e770 26 Kga6s7TalA6w/6C59nKmiKZXhnw fig 3b (comment: WT 11%) ------- COMMENT: 1beda591c5c5e770 27 Kga6s7TalA6w/6C59nKmiKZXhnw fig 3b ((comment: WT 11%) ------- COMMENT: 1beda591c5c5e770 28 ePp0YOfOTv2D+J9pccopDWRT6v0 fig 4a ------- COMMENT: 1beda591c5c5e770 29 ePp0YOfOTv2D+J9pccopDWRT6v0 fig 4a ------- COMMENT: 1beda591c5c5e770 30 0Ljxusd66yE9a+AZriqwWAxBvNM Figure 4A and B ------- COMMENT: 1beda591c5c5e770 31 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: 1beda591c5c5e770 32 CInwmLJEBFXhw6rrcxTEWhlGqI4 Fig 6a ------- COMMENT: 1beda591c5c5e770 33 CInwmLJEBFXhw6rrcxTEWhlGqI4 Fig 6a ------- COMMENT: 1beda591c5c5e770 34 7tfUqKUhn9AWsaT8Hy92HcfS4Fk Figure 7A and B ------- COMMENT: 1beda591c5c5e770 35 7tfUqKUhn9AWsaT8Hy92HcfS4Fk Figure 7A and B ------- COMMENT: 1beda591c5c5e770 36 7tfUqKUhn9AWsaT8Hy92HcfS4Fk Figure 7A and B ------- COMMENT: 1beda591c5c5e770 37 0Ljxusd66yE9a+AZriqwWAxBvNM Figure 4A and B ------- COMMENT: 1c51e41628bda782 1 TKaSPXuTlMdagsvi44b/UDmwjF0 Chromatin immunoprecipitation (ChIP) experiments targeting FLAG-tagged Tor2, Pop3/LST8, Tco89, and Mip1/Raptor, constituents of TORC1,31 exhibited significant TORC1 accumula- tion across the rDNA, predominantly in the 18S and 28S regions (Figures 1A and 1B). ------- COMMENT: 1c51e41628bda782 2 2S+lts5l2+X6HNlVFgaEZGc9AFg Taken together, the transcription of ribosome-associ- ated genes is regulated by TORC1. ------- COMMENT: 1c51e41628bda782 4 ld7XRd+882qf8WI7s9rgZuiqWtY We thus conclude that heterochromatinization of rDNA induced by glucose starvation is initiated by TORC1 inactivation (Figure 3F). ------- COMMENT: 1c51e41628bda782 5 m91LEslvQpvk2Ig8nDC9DLMEd6k Our result indicated that the total amount of 18S rRNA transcripts was reduced by half in the tor2-287 mutant, even under nutrient-rich conditions (Figure 3A). ------- COMMENT: 1c51e41628bda782 6 b+YKfnzKENA8YSwEtcTscCOKMbU Furthermore, we discovered that this reduction was caused by the dissociation of RNA polymerase I from the rDNA region (Figures 3B and S3B). ------- COMMENT: 1c51e41628bda782 7 AhJcTo7Zi8AuBD6i1WTAgIK3xPE heterochromatin forma- tion in rDNA is prompted by the dissociation of the stress- responsive transcription factor Atf1 and the accumulation of the histone chaperone FACT, which maintains H3K9 methyl- ation,40 in addition to the RNAi-dependent pathway.14 We there- fore performed ChIP-qPCR targeting Atf1 and FLAG-tagged Pob3 (a component of FACT), and found that Atf1 diminished from the entire rDNA region, while Pob3-FLAG selectively accu- mulated between rDNA repeats (Figures 3D and 3E). ------- COMMENT: 1c51e41628bda782 8 xuGdddBc6yobyDU88X2bQxf00us To investigate this, we performed ChIP-qPCR tar- geting H3K9 methylation, a marker of heterochromatin forma- tion, in both wild-type and tor2-287 cells. We found that tor2-287 cells exhibited a marked increase in H3K9me2 levels, accompanied by a slight increase in histone H3 occupancy in the rDNA region (Figures 3C and S3C). ------- COMMENT: 1c51e41628bda782 10 ZbXk6DsKTkr6NWXYZLIHswKlIIo we conducted ChIP-qPCR targeting Gcn5-HA and found that it diminished from rDNA in the tor2-287 mutant compared with wild-type (Figure S3D). ------- COMMENT: 1c51e41628bda782 11 PPPFFYinRRwKeaqvyr7AePo8kBY we found that the intracellular Atf1 protein levels were significantly reduced in the tor2-287 mutants when detected with an anti-Atf1 antibody (Figures 3G and 3H). ------- COMMENT: 1c51e41628bda782 12 XVU5nnHIVY8yUIux+zefzS2h0hc We per- formed RT-qPCR and found that transcription of selected ribosome-related genes (rpl102+, rlp7+, and gar2+) was reduced in the tor2-287 mutant (Figure 4A). ------- COMMENT: 1c51e41628bda782 13 XVU5nnHIVY8yUIux+zefzS2h0hc We per- formed RT-qPCR and found that transcription of selected ribosome-related genes (rpl102+, rlp7+, and gar2+) was reduced in the tor2-287 mutant (Figure 4A). ------- COMMENT: 1c51e41628bda782 14 XVU5nnHIVY8yUIux+zefzS2h0hc We per- formed RT-qPCR and found that transcription of selected ribosome-related genes (rpl102+, rlp7+, and gar2+) was reduced in the tor2-287 mutant (Figure 4A). ------- COMMENT: 1c51e41628bda782 16 RvMTRl7eMg//ULnPMYvPtxBO0zw nuc1-632 mutant strain, wherein RNA polymerase I function was impaired.33,34 As a result, we found a considerable reduc- tion in Tor2 accumulation in the rDNA region compared with wild-type cells, concomitant with a decrease in rRNA abundance (Figures 2A and 2B). ------- COMMENT: 1c51e41628bda782 17 CVXnYDc3VTscoiSUnT6CS76Msic Moreover, we found that the diminution of 18S rRNA levels in the tor2-287 mutant could be mitigated by overexpression of Atf1 (atf1 o.p) or disruption of the ago1+ gene (ago1D), which encodes a crucial factor for RNAi-dependent heterochromatinization (Fig- ure 3I). ------- COMMENT: 1c51e41628bda782 27 gkCCXyOP27SVn5XduZn5VwLYOgg Although the downstream S6K kinase Psk1 in the TORC1 pathway has been implicated in RP phosphorylation,39 our finding demon- strated that rRNA abundance was unaffected in the psk1D strain (Figure S3A) ------- COMMENT: 1c51e41628bda782 28 TKaSPXuTlMdagsvi44b/UDmwjF0 Chromatin immunoprecipitation (ChIP) experiments targeting FLAG-tagged Tor2, Pop3/LST8, Tco89, and Mip1/Raptor, constituents of TORC1,31 exhibited significant TORC1 accumula- tion across the rDNA, predominantly in the 18S and 28S regions (Figures 1A and 1B). ------- COMMENT: 1c51e41628bda782 29 QmjM9x3bDZjXUmnCNJOabbF+46s Nevertheless, our analysis revealed that deletion of atf1+ gene (atf1D) did not signif- icantly affect Tor2 accumulation in rDNA (Figures S2A and S2B). ------- COMMENT: 1c51e41628bda782 30 VGlm24oZwKQThr/ALCXsrCxK9KM In addition, the RNA immunoprecipitation analysis revealed a notable association between FLAG-Tor2 and rDNA transcripts, specifically at the 18S, 5.8S, and 28S regions (Figures 2E and S2D) ------- COMMENT: 1c51e41628bda782 31 ms+wyjR/VsfyFGn0egUprXGKdFo We then examined their accumulation at rDNA using ChIP assays and found a decrease in the rDNA accu- mulation of FLAG-Tor2 lacking the HTH domain (Figure 2G). ------- COMMENT: 1c9a3835487bb72e 1 XsCgoNxCeV0kNYTn/TV84QyaArg Table I ------- COMMENT: 1c9a3835487bb72e 2 XsCgoNxCeV0kNYTn/TV84QyaArg Table I ------- COMMENT: 1c9a3835487bb72e 3 XsCgoNxCeV0kNYTn/TV84QyaArg Table I ------- COMMENT: 1c9a3835487bb72e 4 XsCgoNxCeV0kNYTn/TV84QyaArg Table I ------- COMMENT: 1c9a3835487bb72e 5 XsCgoNxCeV0kNYTn/TV84QyaArg Table I ------- COMMENT: 1c9a3835487bb72e 6 XsCgoNxCeV0kNYTn/TV84QyaArg Table I ------- COMMENT: 1c9a3835487bb72e 7 nwvBj4RU+BMkg9dHMmq9ctqTKAI Figures 1A, 1B and S1B - S1I; TableI ------- COMMENT: 1c9a3835487bb72e 8 nwvBj4RU+BMkg9dHMmq9ctqTKAI Figures 1A, 1B and S1B - S1I; TableI ------- COMMENT: 1c9a3835487bb72e 9 nwvBj4RU+BMkg9dHMmq9ctqTKAI Figures 1A, 1B and S1B - S1I; TableI ------- COMMENT: 1c9a3835487bb72e 10 nwvBj4RU+BMkg9dHMmq9ctqTKAI Figures 1A, 1B and S1B - S1I; TableI ------- COMMENT: 1c9a3835487bb72e 11 nwvBj4RU+BMkg9dHMmq9ctqTKAI Figures 1A, 1B and S1B - S1I; TableI ------- COMMENT: 1c9a3835487bb72e 12 nwvBj4RU+BMkg9dHMmq9ctqTKAI Figures 1A, 1B and S1B - S1I; TableI ------- COMMENT: 1c9a3835487bb72e 13 nwvBj4RU+BMkg9dHMmq9ctqTKAI Figures 1A, 1B and S1B - S1I; TableI ------- COMMENT: 1c9a3835487bb72e 14 nwvBj4RU+BMkg9dHMmq9ctqTKAI Figures 1A, 1B and S1B - S1I; TableI ------- COMMENT: 1c9a3835487bb72e 15 nwvBj4RU+BMkg9dHMmq9ctqTKAI Figures 1A, 1B and S1B - S1I; TableI ------- COMMENT: 1c9a3835487bb72e 16 nwvBj4RU+BMkg9dHMmq9ctqTKAI Figures 1A, 1B and S1B - S1I; TableI ------- COMMENT: 1c9a3835487bb72e 17 nwvBj4RU+BMkg9dHMmq9ctqTKAI Figures 1A, 1B and S1B - S1I; TableI ------- COMMENT: 1c9a3835487bb72e 18 nwvBj4RU+BMkg9dHMmq9ctqTKAI Figures 1A, 1B and S1B - S1I; TableI ------- COMMENT: 1c9a3835487bb72e 19 nwvBj4RU+BMkg9dHMmq9ctqTKAI Figures 1A, 1B and S1B - S1I; TableI ------- COMMENT: 1c9a3835487bb72e 20 nwvBj4RU+BMkg9dHMmq9ctqTKAI Figures 1A, 1B and S1B - S1I; TableI ------- COMMENT: 1c9a3835487bb72e 22 phJSS1WoUf9xo3AXbNJUbewZ5EE Figure S1K ------- COMMENT: 1c9a3835487bb72e 23 phJSS1WoUf9xo3AXbNJUbewZ5EE Figure S1K ------- COMMENT: 1c9a3835487bb72e 24 mZnnRcFiXNHEetBNrHmQyPLB8f4 TAble II ------- COMMENT: 1c9a3835487bb72e 25 mZnnRcFiXNHEetBNrHmQyPLB8f4 TAble II ------- COMMENT: 1c9a3835487bb72e 26 mZnnRcFiXNHEetBNrHmQyPLB8f4 TAble II ------- COMMENT: 1c9a3835487bb72e 27 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 1c9a3835487bb72e 28 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 1c9a3835487bb72e 29 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 1c9a3835487bb72e 30 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 1c9a3835487bb72e 31 HlgaHGnb5DOy0MZedtxfsDS404o Figures 2I – 2K and S3G – S3O ------- COMMENT: 1c9a3835487bb72e 32 HlgaHGnb5DOy0MZedtxfsDS404o Figures 2I – 2K and S3G – S3O ------- COMMENT: 1c9a3835487bb72e 33 hrzUoR3GsSv42M/lwMiwOjLnTUc Figures 2L, S3P– S3R ------- COMMENT: 1c9a3835487bb72e 34 hrzUoR3GsSv42M/lwMiwOjLnTUc Figures 2L, S3P– S3R ------- COMMENT: 1c9a3835487bb72e 35 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 1c9a3835487bb72e 36 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 1c9a3835487bb72e 37 3q5nTxn/PvPkm8NqujpAtajxu84 Figures 3B ------- COMMENT: 1c9a3835487bb72e 38 LQmuKZbL9ND56pzzWctDT58tWRE Figure 3C ------- COMMENT: 1c9a3835487bb72e 39 8zdno63XFs3BFsVP161LRtfCMNM Figure 3D ------- COMMENT: 1c9a3835487bb72e 40 C6u0bg8KEYIj6A823ggA1xP/FPc Figure 3G ------- COMMENT: 1c9a3835487bb72e 42 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 1ced65ea728cb6e0 13 67wvBGj+Ov3J0Ku/9JTgCw1F7eY (comment: binds both DNA and histone. Not sure if the H3 preference is an artefact of in vitro system) ------- COMMENT: 1ced65ea728cb6e0 23 67wvBGj+Ov3J0Ku/9JTgCw1F7eY (comment: binds both DNA and histone. Not sure if the H3 preference is an artefact of in vitro system) ------- COMMENT: 1cf182adbddacf73 7 l2TzHPllxPM5hlhy58480H2pcEc (comment: temp semi-permissive for cdc6-23 alone) ------- COMMENT: 1cf182adbddacf73 8 +uFVkvdfTTDgxcP9Nu1yzRvC7+g (comment: temp semi-permissive for pol1-1 alone) ------- COMMENT: 1cf182adbddacf73 9 r2iqrXeeqdaQSqeiYFttiwNFzko (comment: temp semi-permissive for cdc20-M10 alone ------- COMMENT: 1cf182adbddacf73 39 l2TzHPllxPM5hlhy58480H2pcEc (comment: temp semi-permissive for cdc6-23 alone) ------- COMMENT: 1cf182adbddacf73 47 l2TzHPllxPM5hlhy58480H2pcEc (comment: temp semi-permissive for cdc6-23 alone ------- COMMENT: 1cf182adbddacf73 49 l2TzHPllxPM5hlhy58480H2pcEc (comment: temp semi-permissive for cdc6-23 alone ------- COMMENT: 1d0fcf1209195c82 1 +64aznn82Ruby60fOaN1ZiT3rTg (comment: Strong binding with hht3-K9MK14ub peptide) ------- COMMENT: 1d0fcf1209195c82 3 dxu/iahMijJ0I7h8DITsJCclD0A H3K9me3 levels at pericentric dh repeats and dh RNA levels in hht3-K9MK14R cells are similar to those in wild-type cells, comparing with hht3-K9M. ------- COMMENT: 1d0fcf1209195c82 4 umWW1WAjGP/yg9XcrngizGWIbDA Figure 2. ------- COMMENT: 1d0fcf1209195c82 5 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 1d0fcf1209195c82 6 umWW1WAjGP/yg9XcrngizGWIbDA Figure 2. ------- COMMENT: 1d2eeda40cb4401e 2 RPdkqAedukJ0cWGJswoHwJ+rPAE fig. 3 ------- COMMENT: 1d2eeda40cb4401e 5 RPdkqAedukJ0cWGJswoHwJ+rPAE fig. 3 ------- COMMENT: 1d2eeda40cb4401e 8 QIckfZVdn0c0ivoy0+gi871t7AE Supp S14 ------- COMMENT: 1d3f48e98af7ffd6 1 p0wYefXNp0qVluCxxlMo4WTcHEk Therefore, sec9+ is essential for vegetative cell growth and spore germination. ------- COMMENT: 1d3f48e98af7ffd6 2 DZ5DQneTYvgUhdGvu0dF427OWig The level of sec9 mRNA began to increase about 6 hr after induction and peaked at about 9 hr, when cells were in early meiosis II (Fig. 2A, 2B). ------- COMMENT: 1d3f48e98af7ffd6 3 /AIaJkkUVCgHHhL8rQifAhQzqWc As shown in Fig. 2A, accumulation of sec9 mRNA was completely abolished in the mei4Δ mutant. Furthermore, ectopic overexpression of mei4+ was found to induce sec9+ mRNA in vegetative cells (Fig. 2C). sec9+ has a consensus recognition sequence for Mei4, GTAAAYA (Horie et al., 1998) in the 5' upstream region. We conclude that transcription of sec9+ during meiosis is strictly regulated by Mei4. ------- COMMENT: 1d3f48e98af7ffd6 4 /AIaJkkUVCgHHhL8rQifAhQzqWc As shown in Fig. 2A, accumulation of sec9 mRNA was completely abolished in the mei4Δ mutant. Furthermore, ectopic overexpression of mei4+ was found to induce sec9+ mRNA in vegetative cells (Fig. 2C). sec9+ has a consensus recognition sequence for Mei4, GTAAAYA (Horie et al., 1998) in the 5' upstream region. We conclude that transcription of sec9+ during meiosis is strictly regulated by Mei4. ------- COMMENT: 1d3f48e98af7ffd6 5 4zuxDc1r/WRDP+05Gte6CXDai6c In addition to causing a defect in ascospore formation, the sec9-10 mutation compromised vegetative growth. As shown in Fig. 3B, the sec9-10 mutant grew well at 25°C but was unable to form colonies at 37°C. ------- COMMENT: 1d3f48e98af7ffd6 6 rEcFnDzbjR/mBaiByhdFIHicMW4 In marked contrast, sec9-10 cells exhibited a rather uniform arrest morphology at the restrictive temperature (Fig. 3C). At 12 hr after the shift to 34°C, approximately 43% of the sec9-10 cells had a single septum, and 4% exhibited multiple septa (Table II). ------- COMMENT: 1d3f48e98af7ffd6 7 rEcFnDzbjR/mBaiByhdFIHicMW4 In marked contrast, sec9-10 cells exhibited a rather uniform arrest morphology at the restrictive temperature (Fig. 3C). At 12 hr after the shift to 34°C, approximately 43% of the sec9-10 cells had a single septum, and 4% exhibited multiple septa (Table II). ------- COMMENT: 1d3f48e98af7ffd6 8 p0wYefXNp0qVluCxxlMo4WTcHEk Therefore, sec9+ is essential for vegetative cell growth and spore germination. ------- COMMENT: 1d3f48e98af7ffd6 10 wlgcyNWNQUuhAAEpxGWmbbIxi5A In wild type cells, most haploid nuclei produced by meiotic second divisions were encapsulated by the FSM (Fig. 4A). In sec9-10 mutant cells, FSMs initiated normally at both poles of the meiosis II spindles (Fig. 4A), but extension of the FSMs was soon blocked, resulting in anucleated small prespores (Fig. 4B). These results indicated that the FSM initiated normally, but its subsequent development was abnormal. ------- COMMENT: 1d66b48c0e2f2f2b 1 vuc5OtuSTiiYhdgVtk0UXvcexKo ------- COMMENT: 1d66b48c0e2f2f2b 4 DpbI03mvGfGgU7gE6Mo+xI8vS3o ------- COMMENT: 1d66b48c0e2f2f2b 48 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig. 4 ------- COMMENT: 1d66b48c0e2f2f2b 49 4uF9ny9/6JS94LCFegwT9vyY3RY fig. 4 ------- COMMENT: 1d66b48c0e2f2f2b 50 4uF9ny9/6JS94LCFegwT9vyY3RY fig. 4 ------- COMMENT: 1d6c058971c3d808 1 arteRsr/7fVuoEOfiOSAvja8AiA Fig. 1c Serine is the major phosphoamino acid ------- COMMENT: 1d6c058971c3d808 2 Ii86kjkVTcGHkG8zvW2UjS2152Q Fig. 1c threonine is the minor phosphoamino acid ------- COMMENT: 1d6c058971c3d808 3 pV4GZlVu3mX2IQQ3zatgb10cSZQ Fig. 4a Cells blocked in G2 ------- COMMENT: 1d6c058971c3d808 4 2ts/ltJgsNlEZ8rNFsOyOWlv198 Fig. 4a Cells blocked in mitosis ------- COMMENT: 1d8bd8c589d2c2ca 1 x74guHxblAVDorg/5lJR3uT1rwo (comment: Pil1p form filaments. Pil1 exchanges rapidly at the ends of these filaments in vivo) ------- COMMENT: 1d8bd8c589d2c2ca 2 x74guHxblAVDorg/5lJR3uT1rwo (comment: Pil1p form filaments. Pil1 exchanges rapidly at the ends of these filaments in vivo) ------- COMMENT: 1dadbd005c6e53cc 1 s7cvhfiYiveaAA2KzWLwMqq+fAs Fig. 4c ------- COMMENT: 1dadbd005c6e53cc 2 3RfQFZT/EP7C+UY7u9NXb+oN+Jo Fig. 1a. AND fig 4 (knob) ------- COMMENT: 1dadbd005c6e53cc 3 PxGYNeSxrV5PD+XotSQQNBxfnSo Fig. 1a ------- COMMENT: 1dadbd005c6e53cc 4 kAFVhHI5x0BwVja+A0ghrgYmVkc Fig. 3B ------- COMMENT: 1dadbd005c6e53cc 5 1WDivblytTaN4FwtaFlaxijK/wI fig. 3D ------- COMMENT: 1dadbd005c6e53cc 6 G2klmmDu9fwFBecrFe7JYj1fggk fig. S6 check allele???? ------- COMMENT: 1dadbd005c6e53cc 7 kAFVhHI5x0BwVja+A0ghrgYmVkc fig 3B ------- COMMENT: 1dadbd005c6e53cc 8 kAFVhHI5x0BwVja+A0ghrgYmVkc fig 3B ------- COMMENT: 1dadbd005c6e53cc 10 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 1dadbd005c6e53cc 11 s7cvhfiYiveaAA2KzWLwMqq+fAs fig 4c ------- COMMENT: 1dadbd005c6e53cc 13 lVvMgkFNoEp4aURnzoIpcy7J//0 fig 5A ------- COMMENT: 1dadbd005c6e53cc 14 e+l0InXr8+vVi3R3h3XdYa5HYgY fig 5C ------- COMMENT: 1dadbd005c6e53cc 15 lVvMgkFNoEp4aURnzoIpcy7J//0 fig 5A ------- COMMENT: 1dadbd005c6e53cc 16 lVvMgkFNoEp4aURnzoIpcy7J//0 fig 5A ------- COMMENT: 1dadbd005c6e53cc 17 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 1dadbd005c6e53cc 18 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 1dadbd005c6e53cc 19 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 1dadbd005c6e53cc 20 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 1dadbd005c6e53cc 21 aEPFL4f0cMZnJIsdQXUtkYaOqaA Fig. 7a ------- COMMENT: 1dadbd005c6e53cc 22 EijGOzW3mkcolY6w+sPzXrxZE9c Fig. 7b (comment, CHECK increased occurance) ------- COMMENT: 1dadbd005c6e53cc 23 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 1dadbd005c6e53cc 24 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 1dd1a72244ca3e99 6 q3fzVNbBbrjct+tDfeiecX8Ixhc (comment: at telomeres 1L, 2R) ------- COMMENT: 1dd1a72244ca3e99 11 fxf2dH3Fynb+M4kHyh0k4MfdvA0 (comment: at telomere 1R) ------- COMMENT: 1dd1a72244ca3e99 15 WGoyBbd00mmVuOb8gh6dxvGkmQs (comment: greater decrease at telomeres 1R and 2L than at 1L and 2R) ------- COMMENT: 1dd1a72244ca3e99 16 qH93pzXC440lqNSwVe9/MfQTfII (comment: at telomeres 1L, 1R, 2L, 2R) ------- COMMENT: 1de58f8a4e93861a 1 r7zUlF4cTKCpO7m0YuqFgcuegg8 Figure 1A and 1B ------- COMMENT: 1de58f8a4e93861a 2 OhvdBcsL9edlYNW1bq8cDYqXlIY (comment: Does not bind when Cdc15 is phosphorylated by Pom1) ------- COMMENT: 1de58f8a4e93861a 3 9ttcbqySnRlwAGVygO4mu7Zo3w4 (comment: Binds both non phosphorylated Cdc15 and Cdc15 Phosphorylated by Pom1) ------- COMMENT: 1de58f8a4e93861a 6 Jj77wRL1zGDEzNKMyGKyU9n/gHM (comment: CHECK 4B?) ------- COMMENT: 1de58f8a4e93861a 7 SDTFdp8JEStllvLptnOq9x/jWws Though the length of CR formation (node appearance to complete ring) was similar in wild type, cdc15-22A, and cdc15-22D, the periods of maturation (interval between CR formation and constriction initiation) and constriction (start to end of CR diameter decrease) were shorter in cdc15-22A and longer in cdc15-22D (Figure 4F). ------- COMMENT: 1de58f8a4e93861a 12 pQcy/gbCLpr6nkeQxVJtTTOHXtg Indeed, the percentage of tip septa was significantly reduced in mid1Δ pom1as1 cdc15-22D cells (Figure 4G). ------- COMMENT: 1de58f8a4e93861a 14 lPer2ncYxtkCY1bCl13x5ILsRG4 Figure 6E ------- COMMENT: 1de58f8a4e93861a 15 fK0QPSAw0aqQgWn8QHvQF1JoZNw Figure 6D Indeed, deleting components of the CR scaffolded by Cdc15 (e.g., pxl1 or fic1) suppressed tip septation in mid1Δ pom1as1cells to a similar degree as cdc15-22D (Figure 6, D and E). ------- COMMENT: 1de58f8a4e93861a 16 Mc1Dl/8ePjG+W3BQIevC1vaEvqw Figure 2D ------- COMMENT: 1de58f8a4e93861a 17 FSdUZFwpzToCHYa78/AR+nsGsNk Figure 2, C ------- COMMENT: 1de58f8a4e93861a 18 IbovGoYk/uZQUHfFLuUhb0tc0kY the slow-migrating, phosphorylated forms of Cdc15 were reduced in pom1Δ cells (Figure 3B), and recombinant Pom1 efficiently phosphorylated recombinant N-terminal (Cdc15N; amino acids [aa]1–460) ||||||. later....We conclude that Cdc15 is a key substrate in the Pom1-mediated tip occlusion pathway. ------- COMMENT: 1de58f8a4e93861a 20 i34FP9Rrvh33n4Eb72yqslODgHY Figure 6, A and B ------- COMMENT: 1de58f8a4e93861a 21 i34FP9Rrvh33n4Eb72yqslODgHY Figure 6, A and B ------- COMMENT: 1dfcad20e9ecff36 10 eiAlbs0sMLiNPw5hfh80SL9HkME Cnp1 localisation to centromere reduced in teb1-1 cells grown at 36C (based on immunofluorescence) ------- COMMENT: 1dfcad20e9ecff36 15 rY1DItazkS+O1edBskERuc+xJ64 Southern Blot of teb1-1 cells grown at permissive and restrictive temperatures shows no change in telomere length, compared to wild type cells ------- COMMENT: 1dfcad20e9ecff36 20 ZRQBeqdBKeod2VREUYJwXzJyV3I (comment: AACCCT box, subtelomere) ------- COMMENT: 1dfcad20e9ecff36 35 ZRQBeqdBKeod2VREUYJwXzJyV3I (comment: AACCCT box, subtelomere) ------- COMMENT: 1e28c66329150744 1 Ezgv7fPjPkPi8iAX31GrxRO/imw Two transgenes located centromere distal to the tRNA genes were de-repressed in ago1– , dcr1– , and rdp1– , but a transgene located within the central region remained silent. Similar results were obtained in all three mutant strains, as assayed by growth on me- dium lacking uracil and by Northern blots (Fig. 1, B) (21). ------- COMMENT: 1e28c66329150744 2 Ezgv7fPjPkPi8iAX31GrxRO/imw Two transgenes located centromere distal to the tRNA genes were de-repressed in ago1– , dcr1– , and rdp1– , but a transgene located within the central region remained silent. Similar results were obtained in all three mutant strains, as assayed by growth on me- dium lacking uracil and by Northern blots (Fig. 1, B) (21). ------- COMMENT: 1e28c66329150744 3 Ezgv7fPjPkPi8iAX31GrxRO/imw Two transgenes located centromere distal to the tRNA genes were de-repressed in ago1– , dcr1– , and rdp1– , but a transgene located within the central region remained silent. Similar results were obtained in all three mutant strains, as assayed by growth on me- dium lacking uracil and by Northern blots (Fig. 1, B) (21). ------- COMMENT: 1e28c66329150744 4 Ezgv7fPjPkPi8iAX31GrxRO/imw Two transgenes located centromere distal to the tRNA genes were de-repressed in ago1– , dcr1– , and rdp1– , but a transgene located within the central region remained silent. Similar results were obtained in all three mutant strains, as assayed by growth on me- dium lacking uracil and by Northern blots (Fig. 1, B) (21). ------- COMMENT: 1e28c66329150744 5 Ezgv7fPjPkPi8iAX31GrxRO/imw Two transgenes located centromere distal to the tRNA genes were de-repressed in ago1– , dcr1– , and rdp1– , but a transgene located within the central region remained silent. Similar results were obtained in all three mutant strains, as assayed by growth on me- dium lacking uracil and by Northern blots (Fig. 1, B) (21). ------- COMMENT: 1e28c66329150744 6 Ezgv7fPjPkPi8iAX31GrxRO/imw Two transgenes located centromere distal to the tRNA genes were de-repressed in ago1– , dcr1– , and rdp1– , but a transgene located within the central region remained silent. Similar results were obtained in all three mutant strains, as assayed by growth on me- dium lacking uracil and by Northern blots (Fig. 1, B) (21). ------- COMMENT: 1e28c66329150744 7 pZLqiO8ttVXIr29+m9eCyhlS+AI However, three major transcripts that hybridized to the repeats were found to accumulate at high levels in each of the RNAi mutants (Fig. 1C). ------- COMMENT: 1e28c66329150744 8 pZLqiO8ttVXIr29+m9eCyhlS+AI However, three major transcripts that hybridized to the repeats were found to accumulate at high levels in each of the RNAi mutants (Fig. 1C). ------- COMMENT: 1e28c66329150744 9 pZLqiO8ttVXIr29+m9eCyhlS+AI However, three major transcripts that hybridized to the repeats were found to accumulate at high levels in each of the RNAi mutants (Fig. 1C). ------- COMMENT: 1e28c66329150744 10 c6qsOXZ7lonhAty/I81JqB4gWzw These transcripts were also found in swi6– (Fig. 1D) but at a much lower lev ------- COMMENT: 1e28c66329150744 11 frtwX7duxGGz8WbT9oLTNKE+b3E dcr1– , rdp1– , and ago1– cells had increased levels of K4 in the centromeric region in comparison to actin controls (Fig. 3B). ------- COMMENT: 1e28c66329150744 12 frtwX7duxGGz8WbT9oLTNKE+b3E dcr1– , rdp1– , and ago1– cells had increased levels of K4 in the centromeric region in comparison to actin controls (Fig. 3B). ------- COMMENT: 1e28c66329150744 13 frtwX7duxGGz8WbT9oLTNKE+b3E dcr1– , rdp1– , and ago1– cells had increased levels of K4 in the centromeric region in comparison to actin controls (Fig. 3B). ------- COMMENT: 1e28c66329150744 14 rooPv7+sIdl7N2I3WDTTX1Ootfo In contrast, levels of K9 were greatly reduced. ------- COMMENT: 1e28c66329150744 15 rooPv7+sIdl7N2I3WDTTX1Ootfo In contrast, levels of K9 were greatly reduced. ------- COMMENT: 1e28c66329150744 16 rooPv7+sIdl7N2I3WDTTX1Ootfo In contrast, levels of K9 were greatly reduced. ------- COMMENT: 1e28c66329150744 17 V1AOecc+l1kZW0LqejTDKPz/R6E As expected, Swi6, which depends on histone modification for chromatin binding, was de- localized from the ura4 transgenes (Fig. 3C). ------- COMMENT: 1e28c66329150744 18 V1AOecc+l1kZW0LqejTDKPz/R6E As expected, Swi6, which depends on histone modification for chromatin binding, was de- localized from the ura4 transgenes (Fig. 3C). ------- COMMENT: 1e28c66329150744 19 V1AOecc+l1kZW0LqejTDKPz/R6E As expected, Swi6, which depends on histone modification for chromatin binding, was de- localized from the ura4 transgenes (Fig. 3C). ------- COMMENT: 1e2c67b565f85a95 25 hG2daKFoTntWUQ2eI6+eCPKM/SI (comment: same as hsk1-89 alone) ------- COMMENT: 1e2c67b565f85a95 26 hG2daKFoTntWUQ2eI6+eCPKM/SI (comment: same as hsk1-89 alone) ------- COMMENT: 1e2c67b565f85a95 27 hG2daKFoTntWUQ2eI6+eCPKM/SI (comment: same as hsk1-89 alone) ------- COMMENT: 1e2c67b565f85a95 28 hG2daKFoTntWUQ2eI6+eCPKM/SI (comment: same as hsk1-89 alone) ------- COMMENT: 1e2c67b565f85a95 29 hG2daKFoTntWUQ2eI6+eCPKM/SI (comment: same as hsk1-89 alone) ------- COMMENT: 1e2c67b565f85a95 30 hG2daKFoTntWUQ2eI6+eCPKM/SI (comment: same as hsk1-89 alone) ------- COMMENT: 1e2c67b565f85a95 31 hG2daKFoTntWUQ2eI6+eCPKM/SI (comment: same as hsk1-89 alone) ------- COMMENT: 1e2c67b565f85a95 32 hG2daKFoTntWUQ2eI6+eCPKM/SI (comment: same as hsk1-89 alone) ------- COMMENT: 1e2c67b565f85a95 33 hG2daKFoTntWUQ2eI6+eCPKM/SI (comment: same as hsk1-89 alone) ------- COMMENT: 1e6975f2a6cb5e9d 2 PuT8WUVdK+o/1mbOceIy+UMzbGA (comment: snRNA/ complementation of yeast pus1) ------- COMMENT: 1e872f4e7dc98480 16 /ohGydvES38+lMIf0teoALDx6H4 (comment: at 36 degrees Celsius) ------- COMMENT: 1e872f4e7dc98480 17 /ohGydvES38+lMIf0teoALDx6H4 (comment: CONDITION at 36 degrees Celsius) ------- COMMENT: 1e872f4e7dc98480 18 /ohGydvES38+lMIf0teoALDx6H4 (comment: CONDITION at 36 degrees Celsius) ------- COMMENT: 1e872f4e7dc98480 19 /ohGydvES38+lMIf0teoALDx6H4 (comment: CONDITION at 36 degrees Celsius) ------- COMMENT: 1e872f4e7dc98480 20 /ohGydvES38+lMIf0teoALDx6H4 (comment: CONDITION at 36 degrees Celsius) ------- COMMENT: 1e872f4e7dc98480 21 eJTDMAqda+WttGpAejQqlRUjGCQ (comment: CONDITION at 33 degrees Celsius) ------- COMMENT: 1e872f4e7dc98480 22 /ohGydvES38+lMIf0teoALDx6H4 (comment: CONDITION at 36 degrees Celsius) ------- COMMENT: 1e872f4e7dc98480 23 eJTDMAqda+WttGpAejQqlRUjGCQ (comment: CONDITION at 33 degrees Celsius) ------- COMMENT: 1e872f4e7dc98480 24 eJTDMAqda+WttGpAejQqlRUjGCQ (comment: CONDITION at 33 degrees Celsius) ------- COMMENT: 1e872f4e7dc98480 25 eJTDMAqda+WttGpAejQqlRUjGCQ (comment: CONDITION at 33 degrees Celsius) ------- COMMENT: 1e872f4e7dc98480 34 O7BMW6s5ti18AY8rJZAGCe5yBb0 figure 2AB ------- COMMENT: 1e872f4e7dc98480 35 O7BMW6s5ti18AY8rJZAGCe5yBb0 figure 2AB ------- COMMENT: 1e872f4e7dc98480 36 O7BMW6s5ti18AY8rJZAGCe5yBb0 figure 2AB ------- COMMENT: 1e872f4e7dc98480 37 O7BMW6s5ti18AY8rJZAGCe5yBb0 figure 2AB ------- COMMENT: 1e872f4e7dc98480 38 O7BMW6s5ti18AY8rJZAGCe5yBb0 figure 2AB ------- COMMENT: 1e872f4e7dc98480 39 IhRyRyxwj0OnRr2PTWk3kxpyLTY The mitotic spindle has two poles but is thicker than normal. ------- COMMENT: 1e872f4e7dc98480 40 IhRyRyxwj0OnRr2PTWk3kxpyLTY The mitotic spindle has two poles but is thicker than normal. ------- COMMENT: 1eaa4ef71c675ac0 1 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 1eaa4ef71c675ac0 2 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 1eaa4ef71c675ac0 3 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 1eaa4ef71c675ac0 4 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: 1eaa4ef71c675ac0 5 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: 1eaa4ef71c675ac0 6 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: 1eaa4ef71c675ac0 7 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: 1eaa4ef71c675ac0 8 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig 4 ------- COMMENT: 1eaa4ef71c675ac0 9 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig 4 ------- COMMENT: 1eaa4ef71c675ac0 10 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig 4 ------- COMMENT: 1eaa4ef71c675ac0 11 WBzHy33VfmmtIvNGLfqfiUNJGE8 (comment: different pathway) ------- COMMENT: 1eaa4ef71c675ac0 12 WBzHy33VfmmtIvNGLfqfiUNJGE8 (comment: different pathway) ------- COMMENT: 1eaa4ef71c675ac0 13 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 1eaa4ef71c675ac0 14 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 1eaa4ef71c675ac0 15 jsgoOG4IHO0548nzBfjiM4DYmEI fig 5 (comment: during ectopic SIN activation) ------- COMMENT: 1eaa4ef71c675ac0 16 d7119wHfZxrXd2ZqHl5B/nNoqnA Figure 5, D and E ------- COMMENT: 1eaa4ef71c675ac0 17 d7119wHfZxrXd2ZqHl5B/nNoqnA Figure 5, D and E ------- COMMENT: 1eaa4ef71c675ac0 19 ZEwPIZcaxfcMXjkO4hCPOXoUCA0 Figure 6 (comment: asymetric during cytokinesis delay) ------- COMMENT: 1eaa4ef71c675ac0 20 8G6+j/Mkk6lmbSbCeunxDRKglvY Figure 7 ------- COMMENT: 1ebe4a1548de4aff 5 pkn22+OS4tBCPQg1tN2rszTdTeU (comment: thiamine absent; expression level lower than with endogenous promoter but higher than when repressed) ------- COMMENT: 1ebe4a1548de4aff 6 xIrxaTK+kBDcbvAwG+i9JAmQ/g8 (comment: CHECK promoter repressed) ------- COMMENT: 1edc814df6ff1176 3 jgoGuBgb+Ot76esr6oM9CSFJl3I Fig 1E ------- COMMENT: 1edc814df6ff1176 5 /1vUgSGZ7tHAXHyEbfednrSo5Es Fig 2B "Sre1 cleavage defect under low oxygen" ------- COMMENT: 1edc814df6ff1176 6 N5iyUwdxEZ4wQQ1PrkqW4eij1Cs Fig 2C ------- COMMENT: 1edc814df6ff1176 7 N5iyUwdxEZ4wQQ1PrkqW4eij1Cs Fig 2C ------- COMMENT: 1edc814df6ff1176 8 VuXl6YTXs5tHcuGJHmwRlSSzRUk Western blot analysis show Sre1 cleavage defect under low oxygen ------- COMMENT: 1edc814df6ff1176 9 VuXl6YTXs5tHcuGJHmwRlSSzRUk Western blot analysis show Sre1 cleavage defect under low oxygen ------- COMMENT: 1edc814df6ff1176 10 VuXl6YTXs5tHcuGJHmwRlSSzRUk Western blot analysis show Sre1 cleavage defect under low oxygen ------- COMMENT: 1edc814df6ff1176 11 R8AUIAyjQb8W7n04qnI/3DcU5vA Fig 8C 8D Western blot analysis show Sre1 cleavage defect under low oxygen ------- COMMENT: 1edc814df6ff1176 14 1K3960Sr3ZE7Pcuja0xo36sp1Uo Fig 8C 8D Western blot analysis show decreased Sre1 cleavage activation under low oxygen ------- COMMENT: 1edc814df6ff1176 15 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: 1edc814df6ff1176 16 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: 1edc814df6ff1176 17 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: 1edc814df6ff1176 18 /1vUgSGZ7tHAXHyEbfednrSo5Es Fig 2B, "Sre1 cleavage defect under low oxygen" ------- COMMENT: 1edc814df6ff1176 19 Q7VhPVgSGS1zS6C/jDiZsc0sCC0 Fig 2B, lanes 5–13 ------- COMMENT: 1edc814df6ff1176 20 5vor/3JJIP6traYXeD4V7Lyn3ls Fig 2D, lane 3 ------- COMMENT: 1edc814df6ff1176 21 pqfzO63xJyQarHa6VYuxNP/bAtI Fig 2G, lanes 10– 12 ------- COMMENT: 1edc814df6ff1176 22 mXC2VA6/42ypgF6MxYY0NoLM8mM Fig 2G, lanes 6–8 ------- COMMENT: 1edc814df6ff1176 23 cUvka6boYtR0bi2Shy2Xb1sSsp8 Fig 3A, lane 4 both cleavage products ------- COMMENT: 1edc814df6ff1176 24 qvlr/wJL8HhZwhXNi4ZwQ1hT4po Fig 3A, lane 3 ------- COMMENT: 1edc814df6ff1176 25 qvlr/wJL8HhZwhXNi4ZwQ1hT4po Fig 3A, lane 3 ------- COMMENT: 1edc814df6ff1176 26 Rv8sDED3gzJIE7G0YMgCuD+TS0M Fig 3D, compare lanes 3 and 4 ------- COMMENT: 1edc814df6ff1176 27 wbkm41D2NYl/diuPeDG5XjUOGDM Fig 3D ------- COMMENT: 1edc814df6ff1176 28 wbkm41D2NYl/diuPeDG5XjUOGDM Fig 3D ------- COMMENT: 1edc814df6ff1176 29 wbkm41D2NYl/diuPeDG5XjUOGDM Fig 3D ------- COMMENT: 1edc814df6ff1176 30 Rv8sDED3gzJIE7G0YMgCuD+TS0M Fig 3D compare lanes 3 and 4 ------- COMMENT: 1edc814df6ff1176 33 X7OQLyZ0MsKj2OpAnVFixSQSSMc Figure 3 E, precursor ------- COMMENT: 1edc814df6ff1176 34 uYe4Z0ZahibgyFDr3nhtZipkusU Fig 3E and Figure 3 D ------- COMMENT: 1edc814df6ff1176 35 uYe4Z0ZahibgyFDr3nhtZipkusU Fig 3E and Figure 3 D ------- COMMENT: 1edc814df6ff1176 36 KS6gLPY7aW1778JvMdjbYwlfRRw Fig 5D and F (comment: 4.5 fold) precursor) ------- COMMENT: 1edc814df6ff1176 37 gVvWJ1IsWzfvq1sxtecxWd5d6dI PRECURSOR Fig 5E and F ------- COMMENT: 1edc814df6ff1176 38 gVvWJ1IsWzfvq1sxtecxWd5d6dI Fig 5E and F precursor ------- COMMENT: 1edc814df6ff1176 39 gVvWJ1IsWzfvq1sxtecxWd5d6dI Fig 5E and F precursor ------- COMMENT: 1edc814df6ff1176 40 gVvWJ1IsWzfvq1sxtecxWd5d6dI Fig 5E and F precursor ------- COMMENT: 1edc814df6ff1176 41 gVvWJ1IsWzfvq1sxtecxWd5d6dI Fig 5E and F precursor ------- COMMENT: 1edc814df6ff1176 42 gVvWJ1IsWzfvq1sxtecxWd5d6dI Fig 5E and F precursor ------- COMMENT: 1edc814df6ff1176 43 gVvWJ1IsWzfvq1sxtecxWd5d6dI Fig 5E and F precursor ------- COMMENT: 1edc814df6ff1176 44 rZ9OWO2oS9RnhKq3ZNW4131mZYA Fig 5E and F ------- COMMENT: 1edc814df6ff1176 45 MTyEGP4m3aCw3xo9QYvULYrAg5k fig 6 B ------- COMMENT: 1edc814df6ff1176 46 MTyEGP4m3aCw3xo9QYvULYrAg5k Fig 6 B ------- COMMENT: 1edc814df6ff1176 47 MTyEGP4m3aCw3xo9QYvULYrAg5k Fig 6 B ------- COMMENT: 1edc814df6ff1176 48 MTyEGP4m3aCw3xo9QYvULYrAg5k Fig 6 B ------- COMMENT: 1edc814df6ff1176 49 8YnekNLaklRxUOutSRzjlzQvFf0 Fig 7A, lanes 4–6 ------- COMMENT: 1edc814df6ff1176 50 8YnekNLaklRxUOutSRzjlzQvFf0 Fig 7A, lanes 4–6 ------- COMMENT: 1edc814df6ff1176 51 EkDslmRI7jammyNy1lz+ADBpdh8 Fig 8A ------- COMMENT: 1edc814df6ff1176 52 steeyeTIf0tHuMWCSd7Sj4GAFSY Fig 8 ------- COMMENT: 1edc814df6ff1176 53 EkDslmRI7jammyNy1lz+ADBpdh8 Fig 8A ------- COMMENT: 1edc814df6ff1176 54 steeyeTIf0tHuMWCSd7Sj4GAFSY Fig 8 ------- COMMENT: 1edc814df6ff1176 55 MRi+4Qk4t7+nTdnLzmwcwm4AI00 Fig 9A ------- COMMENT: 1edc814df6ff1176 56 MRi+4Qk4t7+nTdnLzmwcwm4AI00 Fig 9A ------- COMMENT: 1edc814df6ff1176 57 MRi+4Qk4t7+nTdnLzmwcwm4AI00 Fig 9A ------- COMMENT: 1edc814df6ff1176 58 MRi+4Qk4t7+nTdnLzmwcwm4AI00 Fig 9A ------- COMMENT: 1edc814df6ff1176 61 cUvka6boYtR0bi2Shy2Xb1sSsp8 Fig 3A, lane 4 both cleavage products ------- COMMENT: 1eee81410bebb43e 14 KvKinkwxAeNXkCpLZRj2Qhvda7c Rad3 phosphorylates S345 in response to DNA damage caused by ionizing radiation ------- COMMENT: 1eee81410bebb43e 17 Uhv0XdZzm49lNp5XztsivcqKfy0 (comment: vw: changed from response to chemical to part of DNA damage checkpoint signalling) ------- COMMENT: 1f11c6ccf7a93b35 2 m1QUaFyXORtoFt+jiIM8g3JGHS8 Fig 6A-B ------- COMMENT: 1f2b998f7febc360 1 TDA+/rVBS5DlZmj5UjsGHJhytEs (Fig. 5G) The IP6-binding pocket formed between CSN2 and Rbx1 is remarkably conserved from yeasts to plants and humans (Figs. 2D and 3D). Deleting ipk1, the yeast IP6 synthase, abolishes Csn2 interaction with Cul1 in Schizosaccharomyces pombe, ------- COMMENT: 1f2b998f7febc360 4 77heESEvL5um3meMT1nexKr8Zyo (Fig. 5H) This defect in UV-resistance can be rescued by wild-type SpCsn2, but not its IP6 binding-deficient K70E mutant . ------- COMMENT: 1f2b998f7febc360 5 77heESEvL5um3meMT1nexKr8Zyo (Fig. 5H) This defect in UV-resistance can be rescued by wild-type SpCsn2, but not its IP6 binding-deficient K70E mutant . ------- COMMENT: 1f2b998f7febc360 6 77heESEvL5um3meMT1nexKr8Zyo (Fig. 5H) This defect in UV-resistance can be rescued by wild-type SpCsn2, but not its IP6 binding-deficient K70E mutant . ------- COMMENT: 1f45b30d29eccb96 15 Gm5TOTFslVTYAxdVRgOMaefCvVg (comment: temperature restrictive for mmi1-ts3) ------- COMMENT: 1f45b30d29eccb96 18 lXhiNjIXELb4F0gNZY7qb2bRpow (comment: pol II localization to sme2 locus) ------- COMMENT: 1f46f7473f4ec0a4 12 uFjLKCYX+wlW2WAhXI6E0cz5CcY ------- COMMENT: 1f6155a543ea7508 63 LxE7z2yVdqI2ricQBDltdOkzY24 (comment: 25S rRNA positions 2216, 2220, 2351) ------- COMMENT: 1f6155a543ea7508 73 ir5fLXCAHfWNzZUo+PtIjVPEVcM (comment: 25S rRNA position 3017) ------- COMMENT: 1f6155a543ea7508 74 MF0YMKHBdSTfpSAxjas3qYCFgxo (comment: 25S rRNA position 1074) ------- COMMENT: 1f6155a543ea7508 75 YQn9V+YfTuKMscVt1KpelN3FsQw (comment: 18S rRNA position 1204) ------- COMMENT: 1f6155a543ea7508 76 CV+dXsTTVTcsRujQZlKjDQlQ1pE (comment: 25S rRNA position 3069) ------- COMMENT: 1f6155a543ea7508 77 medNp3bKR/7ATGf12u8FrPJ6so4 (comment: 25S rRNA positions 2298, 2401) ------- COMMENT: 1f6155a543ea7508 78 hsYArdNAx4kl0TXJ4uLDXnDp23Y (comment: 25S rRNA position 1723) ------- COMMENT: 1f6155a543ea7508 79 UsECkGMSx9Tib+Ce7uOvDRrpCIM (comment: 25S rRNA position 1084) ------- COMMENT: 1f6155a543ea7508 80 lx3gcTBI/EB7fKaQ5/7Z/IXNB3g (comment: 18S rRNA position 1307) ------- COMMENT: 1f6155a543ea7508 81 lwd9IaUZKRcOTGU8VFKNPE8sl2o (comment: 18S rRNA positions 208, 2341) ------- COMMENT: 1f78fe7de80af20c 45 gw0O8W9bwIzuWJaoQtmZDaMKGto ------- COMMENT: 1f7c9dd717c9f378 1 f75rTWBnUsKspBkchc4dLoq0Wdo fig 6b ------- COMMENT: 1f7c9dd717c9f378 2 f75rTWBnUsKspBkchc4dLoq0Wdo fig 6b ------- COMMENT: 1f7c9dd717c9f378 7 02l+3cqTh7UTFJSYjyKhzJabH68 fig 1c ------- COMMENT: 1f7c9dd717c9f378 8 02l+3cqTh7UTFJSYjyKhzJabH68 fig 1c ------- COMMENT: 1f7c9dd717c9f378 9 k1svTtPW1umP07f7OaiPLzxBt7s The only conditions under which we did not detect phosphorylation of Gad8-K263C were in Δtor1 cells in the presence of hydroxyurea or camptothecin (S3B Fig), a finding that may suggest that the activity of the kinase responsible for Gad8-K263C phosphorylation is inhibited under genotoxic stress conditions. ------- COMMENT: 1f7c9dd717c9f378 11 YnsXKNTqhgg1Y46mzZbWNuN+ZjU (comment: affecting substrate Fkh2 in vitro) ------- COMMENT: 1f7c9dd717c9f378 12 YnsXKNTqhgg1Y46mzZbWNuN+ZjU (comment: affecting substrate Fkh2 in vitro) ------- COMMENT: 1f7c9dd717c9f378 13 YnsXKNTqhgg1Y46mzZbWNuN+ZjU (comment: affecting substrate Fkh2 in vitro) ------- COMMENT: 1f7c9dd717c9f378 14 R57eusft1eltzFCHRxNn97TfOvY (comment: CHECK pREP81-gad8-T260C) fig 1a ------- COMMENT: 1f7c9dd717c9f378 15 IdHo0w/qhpaUMIVQFCjhip54hns (comment: CHECK pREP81-gad8-T260C) fig 1a ------- COMMENT: 1f7c9dd717c9f378 16 s/RfsUGDTXV0PllwIH6KL0DDpT0 (comment: CHECK pREP81-gad8-K263C) figure 2 ------- COMMENT: 1f7c9dd717c9f378 17 EWjCn1IG+MJ3/hLTVMHbwDrrBwg (comment: CHECK pREP81-gad8-K263C) ------- COMMENT: 1f7c9dd717c9f378 18 8MevBFWB4W1/0hdpwnmsVAwJcU4 (comment: CHECK pREP81-gad8-T260C) figure 2 ------- COMMENT: 1f7c9dd717c9f378 19 EWjCn1IG+MJ3/hLTVMHbwDrrBwg (comment: pREP81-gad8-K263C) ------- COMMENT: 1f7c9dd717c9f378 20 EWjCn1IG+MJ3/hLTVMHbwDrrBwg (comment: pREP81-gad8-K263C) ------- COMMENT: 1f7c9dd717c9f378 21 1q0ZQLpa3IJQU4PJIzFlgY4X/VI (comment: pREP81-gad8-T260C) fig 2B ------- COMMENT: 1f7c9dd717c9f378 22 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig 2 ------- COMMENT: 1f7c9dd717c9f378 23 OT5lkbopPAiKyovMfAW1CNYm7G4 (comment: pREP81-gad8-Q298L) fig 5a ------- COMMENT: 1f7c9dd717c9f378 24 4AFkNeKdzcep7klxSod/BKx9rlc (comment: pREP81-gad8-Q298L) fig6D ------- COMMENT: 1f7c9dd717c9f378 25 V7OqGc290taTkIHOHUfo39HjtUg (comment: pREP81-gad8-Q298L) ------- COMMENT: 1f7c9dd717c9f378 26 40AkK1CJvXJGMBkYtFzgUqDvD+0 Fig3A. (comment: affecting substrate Fkh2 in vitro) ------- COMMENT: 1f7c9dd717c9f378 27 YnsXKNTqhgg1Y46mzZbWNuN+ZjU (comment: affecting substrate Fkh2 in vitro) ------- COMMENT: 1f7c9dd717c9f378 28 GDFtmbW/aHRyhjF1KzuCYzJRJb4 (comment: affecting Gad8-S546 phosphorylation) ------- COMMENT: 1f7c9dd717c9f378 29 GDFtmbW/aHRyhjF1KzuCYzJRJb4 (comment: affecting Gad8-S546 phosphorylation) ------- COMMENT: 1f7c9dd717c9f378 30 GDFtmbW/aHRyhjF1KzuCYzJRJb4 (comment: affecting Gad8-S546 phosphorylation) ------- COMMENT: 1f7c9dd717c9f378 32 W2V8oVa+mmm7PNF8G0Uc+bMGsyw (comment: Following release from campthotecin) ------- COMMENT: 1f7c9dd717c9f378 33 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: 1f7c9dd717c9f378 34 yyMNf1x4ofpAA8TPiE7bhUbV4SU fig 3b ------- COMMENT: 1f7c9dd717c9f378 35 yyMNf1x4ofpAA8TPiE7bhUbV4SU fig 3b ------- COMMENT: 1f7c9dd717c9f378 36 9iysFi9G+I2IxjrUhGqtPOFC4/E figure 3d. ------- COMMENT: 1f7c9dd717c9f378 37 1q0ZQLpa3IJQU4PJIzFlgY4X/VI (comment: pREP81-gad8-T260C) fig 2B ------- COMMENT: 1f7c9dd717c9f378 38 40AkK1CJvXJGMBkYtFzgUqDvD+0 Fig3A. (comment: affecting substrate Fkh2 in vitro) ------- COMMENT: 1f7c9dd717c9f378 39 k/YFETdeMLPzbGOJ4LRQQH4QMog As previously described [33], mutating both Tor1-dependent phosphorylation sites, S546 and S527, to alanine, abolished the ability of cells to grow at high temperature or in the presence of osmotic stress (Fig 3B) ------- COMMENT: 1f7c9dd717c9f378 41 /IHemTbQpfZYfxvr84BQD+lINH0 Fig3C. we found that Gad8-K263C is phosphorylated at T387 in Δtor1 cells under normal or low-glucose growth conditions. ------- COMMENT: 1f7c9dd717c9f378 42 K4Aw/g60vcEGnoVIdsmJsE1FQJQ Fig3C. The wild type Gad8 is not phosphorylated at T387 in the absence of Tor1 and is only weakly phosphorylated under low-glucose conditions (Fig 3C). ------- COMMENT: 1f7c9dd717c9f378 43 0jU/aop7xttu295uffwk6ch+uHk (S5A Fig) ------- COMMENT: 1f7c9dd717c9f378 44 E/lHOMZPeVa0QTaMZHzJVfsCT6g Additionally, the phosphorylation sites of Gad8 are required for genotoxic stress, since gad8-S527A/S546A mutant alleles are also sensitive to DNA damage and DNA replication stress (S5B Fig). ------- COMMENT: 1f7c9dd717c9f378 45 QMbDFSRr1h/sM+oFT0Hxs3eTWWg fig 6c L fig 6D ------- COMMENT: 1f7c9dd717c9f378 46 E+HwiK6lELoYZwasvyJzkOlnbxY fig 6c (comment: dominent negative effect) ------- COMMENT: 1f7c9dd717c9f378 47 JLM5zvWeJRPzKhEYIu6pjlnxXnE Fig. 7B. ------- COMMENT: 1f7c9dd717c9f378 48 QnEAKBZzuw25LuSzJvPHY2RmqQU Fig 3A. To our surprise, Gad8-K263C was phosphorylated at S546 in wild type cells, as well as in Δtor1 cells (S546-P, Fig 3A).This finding suggests that the Gad8-K263C mutant is phosphorylated by a kinase that normally does not recognize Gad8 as a substrate. ------- COMMENT: 1f7c9dd717c9f378 49 xTfPmD7AYXH5HEWahhWhopN0h+0 Fig 3A.Gad8-K263C was also phosphorylated at S546 under conditions that compromise Tor1 activity, such as osmotic or low glucose stress (S3A Fig), further supporting Tor1-independent phosphorylation of Gad8-K263C by an as yet unknown kinase. ------- COMMENT: 1f7c9dd717c9f378 50 40AkK1CJvXJGMBkYtFzgUqDvD+0 Fig 3A. affecting substrate Fkh2 in vitro ------- COMMENT: 1f7c9dd717c9f378 51 /IHemTbQpfZYfxvr84BQD+lINH0 Fig 3C. we found that Gad8-K263C is phosphorylated at T387 in Δtor1 cells under normal or low-glucose growth conditions. ------- COMMENT: 1f8813e260d783d1 9 Q1QR5qTV0YOJ6VnXH+2Wmg3hhG4 (comment: involved in negative regulation of transcription via transcription factor catabolism) ------- COMMENT: 1f8a9a6747848375 3 IzmknVaS+/cdYHn2JlgyuvLvzGQ (comment: colocalizes with this region and taz1, abnormal localization in taz1-delta, and physically associates with taz1) ------- COMMENT: 1f8a9a6747848375 26 nZuZZ7puyVCnbzLAnpXtOY9KXww (comment: same_pathway) ------- COMMENT: 1f9e5de7abad88fa 3 TiSOxGVVbOuHNenroexZqRqVgOw (comment: assayed using AlF4- to mimic GTP-bound Gpa2) ------- COMMENT: 1fc7b14866f0e4e3 1 vRCG16coFZsUXNQNy9RPWjAHXNI Fig 1 Table1 (comment: affects C1 more than C2 type colonies) ------- COMMENT: 1fc7b14866f0e4e3 2 cIWLBvDO5gGAX5eekoP7B0qIykc Fig 2. ------- COMMENT: 1fc7b14866f0e4e3 4 6Oidriq5GPQktFUxm/WAmQtwlqM Fig S1 ((comment: CHECK decreased aneuploid cell viability during vegetative growth) ------- COMMENT: 1fc7b14866f0e4e3 5 CKbYYtQLe443f9jjRIsQx7WKXcM data not shown ------- COMMENT: 1fc7b14866f0e4e3 6 ZoLOOOqSCGksignQoELRYwqC3Fc Fig S1, (comment: decreased aneuploid cell viability during vegetative growth) ------- COMMENT: 1fc7b14866f0e4e3 7 aa5XqmDedbUFA4BcPMskFjyevNg Fig S1, (comment: decreased aneuploid cell viability during vegetative growth) ------- COMMENT: 1fc7b14866f0e4e3 9 wrog65/DYiKm90g7n3O+DWjioew Fig2. (comment: decreased aneuploid cell viability during vegetative growth) ------- COMMENT: 1fc7b14866f0e4e3 10 cIWLBvDO5gGAX5eekoP7B0qIykc Fig2. ------- COMMENT: 1fc7b14866f0e4e3 11 cIWLBvDO5gGAX5eekoP7B0qIykc Fig2. ------- COMMENT: 1fc7b14866f0e4e3 12 AVMNFhMSARs+/+YvA0nlgCPWGpQ Fig1, Table1 (comment: affects C1 and C2 type colonies) ------- COMMENT: 1fc7b14866f0e4e3 13 LY8RjetZWWDNpdgY0tftICScS7E Fig2. (comment: decreased aneuploid cell viability during vegetative growth) ------- COMMENT: 1fc7b14866f0e4e3 14 wrog65/DYiKm90g7n3O+DWjioew Fig2. (comment: decreased aneuploid cell viability during vegetative growth) ------- COMMENT: 1fc7b14866f0e4e3 15 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 1fc7b14866f0e4e3 16 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 1fc7b14866f0e4e3 17 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 1fc7b14866f0e4e3 18 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 1fc7b14866f0e4e3 19 J59pT8FhzJfGafAUJ/OBmOliAug Fig2 (comment: normal population growth in presence of aneuploid cells) ------- COMMENT: 1fc7b14866f0e4e3 20 J59pT8FhzJfGafAUJ/OBmOliAug Fig2 (comment: normal population growth in presence of aneuploid cells) ------- COMMENT: 1fc7b14866f0e4e3 21 QzrEzK/gBbh/7nK0F0sBJSmTTwc Fig2. (comment: reduced growth may not be specific to aneuploidy as it also interacts with gtb1) ------- COMMENT: 1fc7b14866f0e4e3 22 Xv5Ys3P/xOuZvHIPtWpIztmbspA Fig2. (comment: reduced growth may not be specific to aneuploidy as it also interact with gtb1 though looks quite good to me) ------- COMMENT: 1fc7b14866f0e4e3 23 y5jVjgZ2bUEGZOFS5cn+jtImLPg Table1 Fig1 (comment: affects C1 and C2 type colonies) ------- COMMENT: 1fc7b14866f0e4e3 24 wkOmgZFMWDJmZUf6gbDaXFvRqgk Fig 3A (comment: This strain is disomic for Chromosome 3) ------- COMMENT: 1fc7b14866f0e4e3 25 oHWCEUaleTGyI5fPxievZOrY3kA Fig 3B ((comment: This strain is disomic for Chromosome 3) ------- COMMENT: 1fc7b14866f0e4e3 26 wkOmgZFMWDJmZUf6gbDaXFvRqgk Fig 3A (comment: This strain is disomic for Chromosome 3) ------- COMMENT: 1fc7b14866f0e4e3 27 oHWCEUaleTGyI5fPxievZOrY3kA Fig 3B (comment: This strain is disomic for Chromosome 3) ------- COMMENT: 1fc7b14866f0e4e3 29 GNV5zVEhb22sarLvc3T/d5/en30 Table 2 (comment: This strain is disomic for Chromosome 3) ------- COMMENT: 1fc7b14866f0e4e3 30 YGd7nozS0tmr/eKgxZtFdZpBRA8 Fig 4 (comment: Type C1 colonies have highly elongated cells and are due to various types of aneuploidy. Authors suggest that not3 is required to maintain cell growth) ------- COMMENT: 1fc7b14866f0e4e3 31 ffXBDGLHIQwRHURNOFyNJ6qzAqA (comment: All the genes affect by at least 1.5 fold (141) are reported in Table S2) ------- COMMENT: 1fc7b14866f0e4e3 32 /D6N18Ts6ea3VjF3v6/2wZaKYyQ (comment: All the genes affect by at least 1.5 fold (61) are reported in Table S2) ------- COMMENT: 1fc7b14866f0e4e3 33 3MRZA8S7af45T4e5m6xpBicKR3s (comment: All the genes affect by at least 1.5 fold (17) are reported in Table S2) ------- COMMENT: 1fc7b14866f0e4e3 36 GNV5zVEhb22sarLvc3T/d5/en30 Table 2 (comment: This strain is disomic for Chromosome 3) ------- COMMENT: 1fc7b14866f0e4e3 37 u+TSKKqgm3XoxL8gEW8dxHpjjR4 Fig 6. ------- COMMENT: 1fc7b14866f0e4e3 38 u+TSKKqgm3XoxL8gEW8dxHpjjR4 Fig 6. ------- COMMENT: 1fc7b14866f0e4e3 39 3V3HaMQsZES9F/qR1JrWhOrHbFU Fig 6 ------- COMMENT: 1fc7b14866f0e4e3 40 3V3HaMQsZES9F/qR1JrWhOrHbFU Fig 6 ------- COMMENT: 1fc7b14866f0e4e3 41 3V3HaMQsZES9F/qR1JrWhOrHbFU Fig 6 ------- COMMENT: 1fc7b14866f0e4e3 42 dIO7+LhN35ZV/R/KnjTOkEy92OI Fig 6. (comment: reduced growth may not be specific to aneuploidy as it also interacts with gtb1-93) ------- COMMENT: 1fc7b14866f0e4e3 45 E1icmCHtz5uoVj9w7UplSNM9NsI Fig1, Table1 (comment: affects C1 and C2 type colonies) ------- COMMENT: 1fc7b14866f0e4e3 46 B89FXP7H2BE0TCoiVSJtJVxp+5o Fig1, Table1 (comment: affects C1 type colonies) ------- COMMENT: 1fc7b14866f0e4e3 47 B89FXP7H2BE0TCoiVSJtJVxp+5o Fig1, Table1 (comment: affects C1 type colonies) ------- COMMENT: 1fc7b14866f0e4e3 48 GNV5zVEhb22sarLvc3T/d5/en30 Table 2 (comment: This strain is disomic for Chromosome 3) ------- COMMENT: 1fc7b14866f0e4e3 49 GNV5zVEhb22sarLvc3T/d5/en30 Table 2 (comment: This strain is disomic for Chromosome 3) ------- COMMENT: 1fc9028e783f6d2f 2 /fRbvT0BuvW6J7zmqltzWJPxpjs After release from HU block, cells can undergo one round of division without DNA replication) ------- COMMENT: 1fc9028e783f6d2f 29 01ZNfL5YSr19UUXPr4ELsTmHsDA (comment: Cells unable to divide, even after DNA replication is completed) ------- COMMENT: 1fc9028e783f6d2f 32 01ZNfL5YSr19UUXPr4ELsTmHsDA (comment: Cells unable to divide, even after DNA replication is completed) ------- COMMENT: 1fc9028e783f6d2f 38 5/bTljEdhnePFKMcWRGfHcYQpgk (comment: CHECK abolished) ------- COMMENT: 1fc9028e783f6d2f 63 ZakcCeps8G+f9QjX+ODoYM+RIZ4 (comment: DNA synthesis and cell number increase were rapidly inhibited (data not given but similar to the mitotic mutant cdc2 in Fig. 5 of Nurse et al. 1976)) ------- COMMENT: 1fc9028e783f6d2f 64 ZakcCeps8G+f9QjX+ODoYM+RIZ4 (comment: DNA synthesis and cell number increase were rapidly inhibited (data not given but similar to the mitotic mutant cdc 2 in Fig. 5 of Nurse et al. 1976)) ------- COMMENT: 1fc9028e783f6d2f 65 ZakcCeps8G+f9QjX+ODoYM+RIZ4 (comment: DNA synthesis and cell number increase were rapidly inhibited (data not given but similar to the mitotic mutant cdc 2 in Fig. 5 of Nurse et al. 1976)) ------- COMMENT: 1fc9028e783f6d2f 66 ZakcCeps8G+f9QjX+ODoYM+RIZ4 DNA synthesis and cell number increase were rapidly inhibited (data not given but similar to the mitotic mutant cdc 2 in Fig. 5 of Nurse et al. 1976) ------- COMMENT: 1fc9028e783f6d2f 67 /fRbvT0BuvW6J7zmqltzWJPxpjs (comment: After release from HU block, cells can undergo one round of division without DNA replication) ------- COMMENT: 1fc9028e783f6d2f 68 /fRbvT0BuvW6J7zmqltzWJPxpjs (comment: After release from HU block, cells can undergo one round of division without DNA replication) ------- COMMENT: 1fc9028e783f6d2f 69 /fRbvT0BuvW6J7zmqltzWJPxpjs (comment: After release from HU block, cells can undergo one round of division without DNA replication) ------- COMMENT: 1fc9028e783f6d2f 72 k1QOA4q/eAZap2R5rR0mAMdKvYU (comment: Cells able to undergo normal DNA replication and enter cell division, but fail to divide.) ------- COMMENT: 1fc9028e783f6d2f 73 k1QOA4q/eAZap2R5rR0mAMdKvYU (comment: Cells able to undergo normal DNA replication and enter cell division, but fail to divide.) ------- COMMENT: 1fc9028e783f6d2f 74 b7yjSgwhkZ61nryr0/dUi965p68 (comment: All nuclei took on a granular appearance and in some nuclei this granularity was clearly resolvable into three densely staining bodies resembling condensed chromosomes) (Fig. 3) ------- COMMENT: 1ff973d4c300af54 1 tuSlGCxVQ61bdxwnOMk5tN7iVhY (comment: pMNScdc2-DL5 is integrated Cells observed after 12 hours over expression) Figure 2. (comment: In the paper they call this plasmid pMNSDL5 I have added cdc2-DL5 for clarity) (comment: The pMNS21L plasmid used for isolating this cdc2 mutant has since been rename pREP1.) ------- COMMENT: 1ff973d4c300af54 4 5Nkyi6TKS53HiKcmxaV4oJ4ZclM (comment: pMNScdc2-DL5 fails to rescue cdc2-33 mutant at the restrictive temperature. Do not say how this was assayed) ------- COMMENT: 1ff973d4c300af54 5 Jy8JYcLjXyKdvsynZo1yBAqypRo (comment: pMNScdc2-DL5 is an episomal plasmid.) ------- COMMENT: 1ff973d4c300af54 6 rkaKtP2zq2Q1wteUdvSy9X/VaIk (comment: pMNScdc2-DL5 is integrated cells observed after 30 hours over expression) Figure 2. (comment: In the paper they call this plasmid pMNSDL5 I have added cdc2-DL5 for clarity). (comment: The pMNS21L plasmid used for isolating this cdc2 mutant has since been rename pREP1.) ------- COMMENT: 1ff973d4c300af54 7 Hlb+ktE2/pF+guACMfF67BOsrdA Data not shown. (comment: pMNScdc2-DL5 is integrated) ------- COMMENT: 1ff973d4c300af54 8 Hlb+ktE2/pF+guACMfF67BOsrdA Data not shown. (comment: pMNScdc2-DL5 is integrated) ------- COMMENT: 1ff973d4c300af54 9 Y+60gYVwz7gUwhjrRy4N7f1AD+Q (comment: pMNScdc2-DL5 is integrated) ------- COMMENT: 1ff973d4c300af54 10 MtUJKJ0t6NqmmI6qMGwALes/MgQ (comment: CHECK the endogenous copy of cdc2 has been replaced by cdc2 from human cells CDC2HS. S. pombe cdc2+ is on an episomal plasmid pMNScdc2 Figure 3D) ------- COMMENT: 1ff973d4c300af54 11 i6PeZqMRYZXg1GFz+SUOxx//40A (comment: cdc2-DL5 is over expressed from episomal pMNScdc2DL5. The endogenous cdc2+ has been replaced by the human cdc2 gene CDC2HS.) Same phenotype as shown in Figure 2 ------- COMMENT: 1ff973d4c300af54 12 6SJXM/nO26PeacVHt13GNaMl3kg (comment: CHECK the endogenous copy of cdc2 has been replaced by cdc2 from human cells CDC2HS) (comment: S. pombe cdc2+ is expressed from episomal pMNScdc2 in presence of thiamine. The cdc2 is therefore not really over expressed but I was unable to say it was 'not assayed) Figure 3C ------- COMMENT: 1ff973d4c300af54 13 lqdzrWz/FVlAyxa5uyxTjiOe+mY (comment: CHECK CDC2HS complements cdc2delete phenotype) Figure 4B) ------- COMMENT: 1ff973d4c300af54 16 k/vWsWWcwFR6DSI15ZeEhR0rH7k (comment: CDC2HS is not recognised by anti cdc2 antibody 4711 and so does not contribute to the level of kinase activity assayed.) (comment: S. pombe cdc2+ is on a multi copy plasmid pMNScdc2 Figure 4A lane 1) ------- COMMENT: 1ff973d4c300af54 17 l4aZtbeWi9trwvQRaOJA743K/l4 (comment: cdc2-DL5 is over expressed from episomal pMNScdc2DL5) (comment: CDC2HS is not recognised by anti cdc2 antibody 4711 and so does not contribute to the level of cdc2-DL5 kinase activity assayed.) Figure 4A ------- COMMENT: 1ff973d4c300af54 18 LbbWJF+2sBl6wB+GIsZirwNVgMI (comment: cdc2-DL5 is expressed from episomal pMNScdc2DL5) (comment: CDC2HS is not recognised by anti cdc2 antibody 4711 and so does not contribute to the level of cdc2-DL5 kinase activity Figure 4A) ------- COMMENT: 1ff973d4c300af54 19 7RZEARZzH1CHDuW2WjMXfkREFTQ (comment: cdc13 co immunoprecipitates CDC2HS) Figure 5 ------- COMMENT: 1ff973d4c300af54 20 s7Nsh7nLC3VQ6eeRzFlMHF1GyyU (comment: cdc13 co ips cdc2-DL5 Figure 5) ------- COMMENT: 1ff973d4c300af54 21 riPWjgWAcijxO186DBXQT38JNuQ (comment: co ip of cdc2-DL5 and endogenous CDC2HS with anti cdc13 SP4 shows reduced kinase activity compared to CDC2HS alone Figure 7. cdc2-DL5 is on episomal pMNS cdc2DL5. The authors argue that inactive cdc2-DL5 may titrate away factors required for cdc2 kinase activity but unless I have misunderstood the experiment I think this could just as well be interpreted as SP4 iping a mixture of active and inactive cdc2 kinase activity and thus less total cdc2 kinase activity) ------- COMMENT: 200ad9dc9f52e2f1 1 PxGYNeSxrV5PD+XotSQQNBxfnSo fig 1a ------- COMMENT: 200ad9dc9f52e2f1 2 PxGYNeSxrV5PD+XotSQQNBxfnSo fig 1a ------- COMMENT: 200ad9dc9f52e2f1 3 PxGYNeSxrV5PD+XotSQQNBxfnSo fig 1a ------- COMMENT: 200ad9dc9f52e2f1 4 cqUOKuH1h+wxJebtDP84Ai9Ss6A (comment: t-shift on mitotic entry) Fig. 1d ------- COMMENT: 200ad9dc9f52e2f1 5 cqUOKuH1h+wxJebtDP84Ai9Ss6A (comment: t-shift on mitotic entry) Fig. 1d ------- COMMENT: 200ad9dc9f52e2f1 6 oEO1ssJmfFp/wOFQe+PR6HsjS1A (comment: t-shift on mitotic entry) Fig. 1c ------- COMMENT: 200ad9dc9f52e2f1 7 oEO1ssJmfFp/wOFQe+PR6HsjS1A (comment: t-shift on mitotic entry) Fig. 1c ------- COMMENT: 200ad9dc9f52e2f1 8 oEO1ssJmfFp/wOFQe+PR6HsjS1A (comment: t-shift on mitotic entry) Fig. 1c ------- COMMENT: 200ad9dc9f52e2f1 9 oEO1ssJmfFp/wOFQe+PR6HsjS1A (comment: t-shift on mitotic entry) Fig. 1c ------- COMMENT: 200ad9dc9f52e2f1 10 oEO1ssJmfFp/wOFQe+PR6HsjS1A (comment: t-shift on mitotic entry) Fig. 1c ------- COMMENT: 200ad9dc9f52e2f1 17 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 200ad9dc9f52e2f1 24 qW0fooJwQWtfFzpmyf80rEliP/A fig 5c ------- COMMENT: 200ad9dc9f52e2f1 38 +2XFMV1MQ4r+teF637sQosvJlkk fig 7a ------- COMMENT: 200ad9dc9f52e2f1 39 +2XFMV1MQ4r+teF637sQosvJlkk fig 7a ------- COMMENT: 200ad9dc9f52e2f1 40 +2XFMV1MQ4r+teF637sQosvJlkk fig 7a ------- COMMENT: 200ad9dc9f52e2f1 41 +2XFMV1MQ4r+teF637sQosvJlkk fig 7a ------- COMMENT: 200ad9dc9f52e2f1 42 +2XFMV1MQ4r+teF637sQosvJlkk fig 7a ------- COMMENT: 200ad9dc9f52e2f1 43 +2XFMV1MQ4r+teF637sQosvJlkk fig 7a ------- COMMENT: 200ad9dc9f52e2f1 44 mlfgMGbAfeeYB8+sl8mjlmCas/E ------- COMMENT: 200ad9dc9f52e2f1 45 mlfgMGbAfeeYB8+sl8mjlmCas/E figure 7c, (comment: no rescue of cnd-2) ------- COMMENT: 200ad9dc9f52e2f1 50 bouo1t1XGdH8tn7mAPQjM2nlINs figure 7e ------- COMMENT: 20128de7e05e2f96 41 o1gJQzXM9pAsWA4jZKNaJd54aPM Fig. S3D ------- COMMENT: 2039b43f720bf7a4 7 7Jxhj/2/IJNaoiDUQH++xGW9xoA (comment: same as plo1-ts35 alone) ------- COMMENT: 2041b5cb2443f86c 17 DPLbs6Enkiy8CYBDuuomqYqSKLE ------- COMMENT: 2041b5cb2443f86c 28 usAQkfhl/L/KNTznP68nbn65j0Y ------- COMMENT: 2041b5cb2443f86c 29 f+YkintkqiAx3Xbl3GApHp3NIaw ------- COMMENT: 205df3a82b504d79 34 xqjTztL6qGqaPgp6WgcG+iOomGI (Fig. S1B) ------- COMMENT: 205df3a82b504d79 35 xqjTztL6qGqaPgp6WgcG+iOomGI (Fig. S1B) ------- COMMENT: 205df3a82b504d79 36 xqjTztL6qGqaPgp6WgcG+iOomGI (Fig. S1B) ------- COMMENT: 205df3a82b504d79 37 xqjTztL6qGqaPgp6WgcG+iOomGI (Fig. S1B) ------- COMMENT: 205df3a82b504d79 41 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 205df3a82b504d79 43 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 205df3a82b504d79 44 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 205df3a82b504d79 45 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 205df3a82b504d79 46 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 205df3a82b504d79 47 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 205df3a82b504d79 48 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 205df3a82b504d79 65 UecLDdbzEF2Fd34z5as/4/0I/eg (Fig. S1E) ------- COMMENT: 205df3a82b504d79 74 rzoapsxxo5AQc2NAakt5rfXh8WQ Figure S2E ------- COMMENT: 205df3a82b504d79 76 +Bne5GtIXMkOpJRPgE/AS5nNCM0 (Fig. 2h) ------- COMMENT: 205df3a82b504d79 77 +Bne5GtIXMkOpJRPgE/AS5nNCM0 (Fig. 2h) ------- COMMENT: 205df3a82b504d79 78 +Bne5GtIXMkOpJRPgE/AS5nNCM0 (Fig. 2h) ------- COMMENT: 205df3a82b504d79 79 186mq7DkgI2sp2AOhV+Nj8Wmrt8 (Fig. 2i) ------- COMMENT: 205df3a82b504d79 82 Chuua0tqkfujQK5J6PM8W4jmKfQ (Fig. 3a) ------- COMMENT: 205df3a82b504d79 83 xDMlyIUPFAjT8Q/rT0ka6sjPY78 (Fig. S1C, 3A) ------- COMMENT: 205df3a82b504d79 88 I6643h1q4gduoO79SWdzD0HhtlY Figure 3F and G ------- COMMENT: 205df3a82b504d79 94 VLKUZYHmzkL2AwLQWJb39ONSi8o (comment: vw edited based on https://github.com/geneontology/go-annotation/issues/5239) ------- COMMENT: 205df3a82b504d79 101 GPVllzcmfyoMWbPscsYpY/jnxJE Supp S4e ------- COMMENT: 205df3a82b504d79 102 GPVllzcmfyoMWbPscsYpY/jnxJE Supp S4e ------- COMMENT: 205df3a82b504d79 121 +Op4KC2Y1ceXgMeWIltJ2hBOJwY Fig 5 (comment: vw, added substrate top2, this term will probably merge into displacement activity) ------- COMMENT: 205df3a82b504d79 124 VLKUZYHmzkL2AwLQWJb39ONSi8o (comment: vw edited based on https://github.com/geneontology/go-annotation/issues/5239) ------- COMMENT: 20a419e583ad61e6 1 DgYC4l5Xx1G60lt5ObAIbJeDSXE (comment: cut and uneven nuclear division in cdc21-M68-ts-degron is decreased by cds1 deletion) ------- COMMENT: 20a419e583ad61e6 2 GmRIO4p5lxsZ8UwVKXhDd1F8bqQ (comment: Decrease in RPA accumulation and localization to nuclear periphery in cdc21-M68-ts-degron by pku70 deletion) ------- COMMENT: 20a419e583ad61e6 3 AiZI1zrZUbs9kx0vGbYoaHnDDPY (comment: pKu foci and megafocus RPA is decreased in cdc21-M68-ts-degron by exo1 deletion by) ------- COMMENT: 20a419e583ad61e6 6 1svFIcm95gPRFw0tHFTERvlWs24 (comment: HU absent) ------- COMMENT: 20a419e583ad61e6 15 1svFIcm95gPRFw0tHFTERvlWs24 (comment: HU absent) ------- COMMENT: 20a419e583ad61e6 17 1svFIcm95gPRFw0tHFTERvlWs24 (comment: HU absent) ------- COMMENT: 20a419e583ad61e6 44 1svFIcm95gPRFw0tHFTERvlWs24 (comment: HU absent) ------- COMMENT: 20c200984ffb7421 15 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig 4 ------- COMMENT: 20c200984ffb7421 17 j7XSNPouyXOGc0Se3rMJh+k5V0A fig 9 (comment: high overexpression is lethal) ------- COMMENT: 20c200984ffb7421 20 JvqKiF7syn97ZfVC9qlsi0ZVvfw fig 9 ------- COMMENT: 20c200984ffb7421 21 l+f1R919mLn1hkTFl9hvZaQF59A fig 6 ------- COMMENT: 20c200984ffb7421 22 l+f1R919mLn1hkTFl9hvZaQF59A fig 6 ------- COMMENT: 20c200984ffb7421 23 l+f1R919mLn1hkTFl9hvZaQF59A fig 6 ------- COMMENT: 20f80169cf2b419d 1 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 20f80169cf2b419d 2 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 20f80169cf2b419d 3 dxo7r+lXjKwHgb+2MHEfa/t9/Q8 (Fig. 2D) (comment: additive) ------- COMMENT: 20f80169cf2b419d 4 7IzPVIuZhuKqIVjB4l/cExHiwb8 fig 2D ------- COMMENT: 20f80169cf2b419d 7 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: 20f80169cf2b419d 8 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 20f80169cf2b419d 9 QnjxaEpmXofCxq7boZSb8VJRhoc Fig. 2C ------- COMMENT: 20f80169cf2b419d 10 5DWqo03puFeQtodWqcCbmJ6nfyM fig 1A ------- COMMENT: 20f80169cf2b419d 11 PxGYNeSxrV5PD+XotSQQNBxfnSo fig 1a ------- COMMENT: 20f80169cf2b419d 13 z3Tb8DCX85pB7PVzwmjQAqL+YMM Fig. 3A,B ------- COMMENT: 20f80169cf2b419d 14 hm3RsSqZiTXI8/iZphXE9kPa5tM Fig. 3C ------- COMMENT: 20f80169cf2b419d 15 G4r1MVeP69hdyagwAGh9eH3xSv0 Fig3 D ------- COMMENT: 20f80169cf2b419d 16 mNCjDdhIpLSxJz5CnJvZHOy0PGA fig 3C ------- COMMENT: 20f80169cf2b419d 18 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 20f80169cf2b419d 22 G4r1MVeP69hdyagwAGh9eH3xSv0 Fig3 D ------- COMMENT: 20f80169cf2b419d 23 G4r1MVeP69hdyagwAGh9eH3xSv0 Fig3 D ------- COMMENT: 20f80169cf2b419d 25 P7VYIKmFap++9v83GA/5hvSJ/7w fig 3C ------- COMMENT: 20f80169cf2b419d 26 albsh4c4fJ5n6/A0pXFq63upAmc fig 3C ------- COMMENT: 20f80169cf2b419d 27 albsh4c4fJ5n6/A0pXFq63upAmc fig 3C ------- COMMENT: 20f80169cf2b419d 28 albsh4c4fJ5n6/A0pXFq63upAmc fig 3C ------- COMMENT: 20f80169cf2b419d 29 cr8tXagLo33x5e6AMPW1NzYklX8 fig 3C ------- COMMENT: 20f80169cf2b419d 30 albsh4c4fJ5n6/A0pXFq63upAmc fig 3C ------- COMMENT: 20f80169cf2b419d 31 f75rTWBnUsKspBkchc4dLoq0Wdo fig 6b ------- COMMENT: 20f80169cf2b419d 32 UnQw94nU5T+QzGDesDT6Zls7XHA fig 7B ------- COMMENT: 20f80169cf2b419d 33 UnQw94nU5T+QzGDesDT6Zls7XHA fig 7B ------- COMMENT: 20f80169cf2b419d 34 7N8hCskP8URgzp54ufOYCNWpyaw fig 9D ------- COMMENT: 20f80169cf2b419d 35 zcpaffBwA5YsbrMf/pzCN2i/Nz4 fig 9D ------- COMMENT: 20f80169cf2b419d 36 zcpaffBwA5YsbrMf/pzCN2i/Nz4 fig 9D ------- COMMENT: 20f80169cf2b419d 37 zcpaffBwA5YsbrMf/pzCN2i/Nz4 fig 9D ------- COMMENT: 20f80169cf2b419d 39 B6hB+dh/l8xfahcbXJR8dtDEZgA Fig 8B/tableB ------- COMMENT: 20f80169cf2b419d 40 B6hB+dh/l8xfahcbXJR8dtDEZgA Fig 8B/tableB ------- COMMENT: 20f80169cf2b419d 41 B6hB+dh/l8xfahcbXJR8dtDEZgA Fig 8B/tableB ------- COMMENT: 20f80169cf2b419d 42 lC9MJNTC0RK0K7e90ci4GkCQiNw fig 9A ------- COMMENT: 21220c7b23915d21 2 9r6jpejM/rwYANTnxF24Sa98+EE (comment: actually 25 degrees, but calling it low to make distinction from inviable at 30) ------- COMMENT: 21220c7b23915d21 3 ewfspod7Bin5Lx5/B/NV8EarpfI (comment: CONDITION 30 degrees) ------- COMMENT: 21220c7b23915d21 4 JfOMB/VihzDL3xvBrspH9ZwMq5M (comment: 25 degrees, but calling it low to make distinction from inviable at 30) ------- COMMENT: 21220c7b23915d21 5 JfOMB/VihzDL3xvBrspH9ZwMq5M (comment: 25 degrees, but calling it low to make distinction from inviable at 30) ------- COMMENT: 21220c7b23915d21 6 JfOMB/VihzDL3xvBrspH9ZwMq5M (comment: 25 degrees, but calling it low to make distinction from inviable at 30) ------- COMMENT: 21220c7b23915d21 12 9r6jpejM/rwYANTnxF24Sa98+EE (comment: actually 25 degrees, but calling it low to make distinction from inviable at 30) ------- COMMENT: 21220c7b23915d21 14 9r6jpejM/rwYANTnxF24Sa98+EE (comment: actually 25 degrees, but calling it low to make distinction from inviable at 30) ------- COMMENT: 21220c7b23915d21 23 sqhRluliQav0pKKLQW0W2t0Apis (comment: 25 degrees, permissive for hsk1-89) ------- COMMENT: 21220c7b23915d21 24 sqhRluliQav0pKKLQW0W2t0Apis (comment: 25 degrees, permissive for hsk1-89) ------- COMMENT: 21220c7b23915d21 25 sqhRluliQav0pKKLQW0W2t0Apis (comment: 25 degrees, permissive for hsk1-89) ------- COMMENT: 21220c7b23915d21 27 sqhRluliQav0pKKLQW0W2t0Apis (comment: 25 degrees, permissive for hsk1-89) ------- COMMENT: 21220c7b23915d21 28 sqhRluliQav0pKKLQW0W2t0Apis (comment: 25 degrees, permissive for hsk1-89) ------- COMMENT: 21220c7b23915d21 29 sqhRluliQav0pKKLQW0W2t0Apis (comment: 25 degrees, permissive for hsk1-89) ------- COMMENT: 21220c7b23915d21 30 sqhRluliQav0pKKLQW0W2t0Apis (comment: 25 degrees, permissive for hsk1-89) ------- COMMENT: 21220c7b23915d21 40 IiP/irQ4sL+8BZDehvIjzlm2TLc comment: sensitivity to HU, MMS, or UV; growth on YES ------- COMMENT: 21220c7b23915d21 41 8LWYRUWKHSRCJomAjlHhJhdHLrM (comment: sensitivity to HU, MMS, or UV) ------- COMMENT: 21220c7b23915d21 42 tJo+1DKUzPTHVxfiuOtyFRQE47M (comment: increased chromatin association in presence of HU) ------- COMMENT: 216f4dec12ef6153 11 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 12 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 216f4dec12ef6153 14 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 216f4dec12ef6153 15 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 216f4dec12ef6153 16 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 216f4dec12ef6153 17 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 18 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 216f4dec12ef6153 19 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 216f4dec12ef6153 20 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 21 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 216f4dec12ef6153 22 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 216f4dec12ef6153 23 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 24 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 216f4dec12ef6153 25 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 216f4dec12ef6153 26 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 27 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 216f4dec12ef6153 28 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 216f4dec12ef6153 29 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 30 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 31 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 32 lMlH9sd8X+YmRwBXRLEfBt2R0vU Supplemental Figure S3B ------- COMMENT: 216f4dec12ef6153 34 lMlH9sd8X+YmRwBXRLEfBt2R0vU Supplemental Figure S3B ------- COMMENT: 216f4dec12ef6153 35 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 36 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 37 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 38 lMlH9sd8X+YmRwBXRLEfBt2R0vU Supplemental Figure S3B ------- COMMENT: 216f4dec12ef6153 39 lMlH9sd8X+YmRwBXRLEfBt2R0vU Supplemental Figure S3B ------- COMMENT: 216f4dec12ef6153 41 lMlH9sd8X+YmRwBXRLEfBt2R0vU Supplemental Figure S3B ------- COMMENT: 216f4dec12ef6153 42 lMlH9sd8X+YmRwBXRLEfBt2R0vU Supplemental Figure S3B ------- COMMENT: 216f4dec12ef6153 44 lMlH9sd8X+YmRwBXRLEfBt2R0vU Supplemental Figure S3B ------- COMMENT: 216f4dec12ef6153 45 lMlH9sd8X+YmRwBXRLEfBt2R0vU Supplemental Figure S3B ------- COMMENT: 216f4dec12ef6153 47 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 48 lMlH9sd8X+YmRwBXRLEfBt2R0vU Supplemental Figure S3B ------- COMMENT: 216f4dec12ef6153 49 lMlH9sd8X+YmRwBXRLEfBt2R0vU Supplemental Figure S3B ------- COMMENT: 216f4dec12ef6153 50 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 51 lMlH9sd8X+YmRwBXRLEfBt2R0vU Supplemental Figure S3B ------- COMMENT: 216f4dec12ef6153 52 lMlH9sd8X+YmRwBXRLEfBt2R0vU Supplemental Figure S3B ------- COMMENT: 216f4dec12ef6153 53 dhQoHOIZ07s8wtq+ylvVKnY5d28 Supplemental Figure S2B ------- COMMENT: 216f4dec12ef6153 54 dhQoHOIZ07s8wtq+ylvVKnY5d28 Supplemental Figure S2B ------- COMMENT: 216f4dec12ef6153 55 dhQoHOIZ07s8wtq+ylvVKnY5d28 Supplemental Figure S2B ------- COMMENT: 216f4dec12ef6153 56 dhQoHOIZ07s8wtq+ylvVKnY5d28 Supplemental Figure S2B ------- COMMENT: 216f4dec12ef6153 57 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 58 dhQoHOIZ07s8wtq+ylvVKnY5d28 Supplemental Figure S2B ------- COMMENT: 216f4dec12ef6153 59 dhQoHOIZ07s8wtq+ylvVKnY5d28 Supplemental Figure S2B ------- COMMENT: 216f4dec12ef6153 60 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 61 dhQoHOIZ07s8wtq+ylvVKnY5d28 Supplemental Figure S2B ------- COMMENT: 216f4dec12ef6153 62 dhQoHOIZ07s8wtq+ylvVKnY5d28 Supplemental Figure S2B ------- COMMENT: 216f4dec12ef6153 63 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 64 dhQoHOIZ07s8wtq+ylvVKnY5d28 Supplemental Figure S2B ------- COMMENT: 216f4dec12ef6153 65 dhQoHOIZ07s8wtq+ylvVKnY5d28 Supplemental Figure S2B ------- COMMENT: 216f4dec12ef6153 66 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 67 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 68 dhQoHOIZ07s8wtq+ylvVKnY5d28 Supplemental Figure S2B ------- COMMENT: 216f4dec12ef6153 69 dhQoHOIZ07s8wtq+ylvVKnY5d28 Supplemental Figure S2B ------- COMMENT: 216f4dec12ef6153 70 Ozzcl6i44uWpA0aTFC+mIS7xFCY Supplemental Figure S1B ------- COMMENT: 216f4dec12ef6153 71 Ozzcl6i44uWpA0aTFC+mIS7xFCY Supplemental Figure S1B ------- COMMENT: 216f4dec12ef6153 72 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 73 Ozzcl6i44uWpA0aTFC+mIS7xFCY Supplemental Figure S1B ------- COMMENT: 216f4dec12ef6153 74 Ozzcl6i44uWpA0aTFC+mIS7xFCY Supplemental Figure S1B ------- COMMENT: 216f4dec12ef6153 75 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 76 Ozzcl6i44uWpA0aTFC+mIS7xFCY Supplemental Figure S1B ------- COMMENT: 216f4dec12ef6153 77 Ozzcl6i44uWpA0aTFC+mIS7xFCY Supplemental Figure S1B ------- COMMENT: 216f4dec12ef6153 78 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 79 Ozzcl6i44uWpA0aTFC+mIS7xFCY Supplemental Figure S1B ------- COMMENT: 216f4dec12ef6153 80 Ozzcl6i44uWpA0aTFC+mIS7xFCY Supplemental Figure S1B ------- COMMENT: 216f4dec12ef6153 81 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 82 Ozzcl6i44uWpA0aTFC+mIS7xFCY Supplemental Figure S1B ------- COMMENT: 216f4dec12ef6153 83 Ozzcl6i44uWpA0aTFC+mIS7xFCY Supplemental Figure S1B ------- COMMENT: 216f4dec12ef6153 84 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 85 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 86 Ozzcl6i44uWpA0aTFC+mIS7xFCY Supplemental Figure S1B ------- COMMENT: 216f4dec12ef6153 87 Ozzcl6i44uWpA0aTFC+mIS7xFCY Supplemental Figure S1B ------- COMMENT: 216f4dec12ef6153 88 LuOBeEWUH8iO4POPR82cY4+psOo Supplemental Figure S6B ------- COMMENT: 216f4dec12ef6153 89 LuOBeEWUH8iO4POPR82cY4+psOo Supplemental Figure S6B ------- COMMENT: 216f4dec12ef6153 90 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 91 LuOBeEWUH8iO4POPR82cY4+psOo Supplemental Figure S6B ------- COMMENT: 216f4dec12ef6153 92 LuOBeEWUH8iO4POPR82cY4+psOo Supplemental Figure S6B ------- COMMENT: 216f4dec12ef6153 93 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 94 LuOBeEWUH8iO4POPR82cY4+psOo Supplemental Figure S6B ------- COMMENT: 216f4dec12ef6153 95 LuOBeEWUH8iO4POPR82cY4+psOo Supplemental Figure S6B ------- COMMENT: 216f4dec12ef6153 96 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 97 LuOBeEWUH8iO4POPR82cY4+psOo Supplemental Figure S6B ------- COMMENT: 216f4dec12ef6153 98 LuOBeEWUH8iO4POPR82cY4+psOo Supplemental Figure S6B ------- COMMENT: 216f4dec12ef6153 99 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 100 LuOBeEWUH8iO4POPR82cY4+psOo Supplemental Figure S6B ------- COMMENT: 216f4dec12ef6153 101 LuOBeEWUH8iO4POPR82cY4+psOo Supplemental Figure S6B ------- COMMENT: 216f4dec12ef6153 102 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 103 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 104 LuOBeEWUH8iO4POPR82cY4+psOo Supplemental Figure S6B ------- COMMENT: 216f4dec12ef6153 105 LuOBeEWUH8iO4POPR82cY4+psOo Supplemental Figure S6B ------- COMMENT: 216f4dec12ef6153 106 ta7Nx6I9bRHFYJqtbPHryfURqug Supplemental Figure S4B ------- COMMENT: 216f4dec12ef6153 107 ta7Nx6I9bRHFYJqtbPHryfURqug Supplemental Figure S4B ------- COMMENT: 216f4dec12ef6153 108 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 109 ta7Nx6I9bRHFYJqtbPHryfURqug Supplemental Figure S4B ------- COMMENT: 216f4dec12ef6153 110 ta7Nx6I9bRHFYJqtbPHryfURqug Supplemental Figure S4B ------- COMMENT: 216f4dec12ef6153 111 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 112 ta7Nx6I9bRHFYJqtbPHryfURqug Supplemental Figure S4B ------- COMMENT: 216f4dec12ef6153 113 ta7Nx6I9bRHFYJqtbPHryfURqug Supplemental Figure S4B ------- COMMENT: 216f4dec12ef6153 114 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 115 ta7Nx6I9bRHFYJqtbPHryfURqug Supplemental Figure S4B ------- COMMENT: 216f4dec12ef6153 116 ta7Nx6I9bRHFYJqtbPHryfURqug Supplemental Figure S4B ------- COMMENT: 216f4dec12ef6153 117 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 118 ta7Nx6I9bRHFYJqtbPHryfURqug Supplemental Figure S4B ------- COMMENT: 216f4dec12ef6153 119 ta7Nx6I9bRHFYJqtbPHryfURqug Supplemental Figure S4B ------- COMMENT: 216f4dec12ef6153 120 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 121 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 122 ta7Nx6I9bRHFYJqtbPHryfURqug Supplemental Figure S4B ------- COMMENT: 216f4dec12ef6153 123 ta7Nx6I9bRHFYJqtbPHryfURqug Supplemental Figure S4B ------- COMMENT: 216f4dec12ef6153 124 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 216f4dec12ef6153 125 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 216f4dec12ef6153 126 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 127 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 216f4dec12ef6153 128 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 216f4dec12ef6153 129 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 130 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 216f4dec12ef6153 131 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 216f4dec12ef6153 132 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 133 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 216f4dec12ef6153 134 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 216f4dec12ef6153 135 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 136 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 216f4dec12ef6153 137 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 216f4dec12ef6153 138 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 139 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 140 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 216f4dec12ef6153 141 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 216f4dec12ef6153 142 hjoecfnoHUhyIO2Fkxl28REj6W4 Supplemental Figure S5B ------- COMMENT: 216f4dec12ef6153 143 hjoecfnoHUhyIO2Fkxl28REj6W4 Supplemental Figure S5B ------- COMMENT: 216f4dec12ef6153 144 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 145 hjoecfnoHUhyIO2Fkxl28REj6W4 Supplemental Figure S5B ------- COMMENT: 216f4dec12ef6153 146 hjoecfnoHUhyIO2Fkxl28REj6W4 Supplemental Figure S5B ------- COMMENT: 216f4dec12ef6153 147 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 148 hjoecfnoHUhyIO2Fkxl28REj6W4 Supplemental Figure S5B ------- COMMENT: 216f4dec12ef6153 149 hjoecfnoHUhyIO2Fkxl28REj6W4 Supplemental Figure S5B ------- COMMENT: 216f4dec12ef6153 150 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 151 hjoecfnoHUhyIO2Fkxl28REj6W4 Supplemental Figure S5B ------- COMMENT: 216f4dec12ef6153 152 hjoecfnoHUhyIO2Fkxl28REj6W4 Supplemental Figure S5B ------- COMMENT: 216f4dec12ef6153 153 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 154 hjoecfnoHUhyIO2Fkxl28REj6W4 Supplemental Figure S5B ------- COMMENT: 216f4dec12ef6153 155 hjoecfnoHUhyIO2Fkxl28REj6W4 Supplemental Figure S5B ------- COMMENT: 216f4dec12ef6153 156 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 157 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 216f4dec12ef6153 158 hjoecfnoHUhyIO2Fkxl28REj6W4 Supplemental Figure S5B ------- COMMENT: 216f4dec12ef6153 159 hjoecfnoHUhyIO2Fkxl28REj6W4 Supplemental Figure S5B ------- COMMENT: 219e86a87274fe5f 27 EwjJ0WM1/1tOgPX5DjDFVa0mIhE (comment: response curve differs from wt and other git mutants) ------- COMMENT: 21c36c32abbfd61b 15 ec6Pexk3YIDQbVlZSI+qHvnsa4A The phenotype is assessed by the high-throughput sequencing. ------- COMMENT: 21d843433d804639 1 0jQjX5UY3MCsqVzo4iLwn4GTHtM Extended Data Figure 2 (comment: nucleoplasmic side) ------- COMMENT: 21d843433d804639 5 ypdJpeySfW15WZbwAcpizEHnaTA Fig. 4, Extended Data Fig. 7 ------- COMMENT: 21d843433d804639 7 fV9E34C1e32Zte0OXoiHFJrNUT8 Extended Data fig4 ------- COMMENT: 21d843433d804639 8 5F9gaGE5tGuTM2hM+YZf7gbVA58 fig 4/6 ------- COMMENT: 21d843433d804639 9 z64ljinkaOnVKRPJgomWWmuww6c Extended Data Figure 8 ------- COMMENT: 21d843433d804639 11 XLR6buAGz5NwhGFfdrfmMwBXcMI fig 3g ------- COMMENT: 21d843433d804639 13 CSVdQFPolHfLAA8C+jGwML9QIdI Les1 stalks functionally isolate daughter nuclei from the process of Imp1-dependent local NEB at the centre of the bridge prob- ably acts to create a seal by gathering the inner nuclear envelope tightly around the spindle ------- COMMENT: 21d843433d804639 15 xG4y4VNKDQO14a4DF1etCgGVpRU Fig. 4a, b. Daughter nuclei in the les1Δ strain also suffered transient leakages at the time of maximum spindle elongation, as measured by loss of nuclear NLS–GFP ------- COMMENT: 21d843433d804639 16 cN/Dtjgbc+RTYCBpo4OV3fhmwvo extended data 6 c,d ------- COMMENT: 21d843433d804639 17 XM80Cz+0SI2JglqWOboANKyzpc0 Fig. 3a, Extended Data Fig. 4a–c Removed from nuclear basket in bridge midzone during nuclear division ------- COMMENT: 21d843433d804639 18 XM80Cz+0SI2JglqWOboANKyzpc0 Fig. 3a, Extended Data Fig. 4a–c Removed from nuclear basket in bridge midzone during nuclear division ------- COMMENT: 21d843433d804639 19 5F9gaGE5tGuTM2hM+YZf7gbVA58 fig 4/6 ------- COMMENT: 21d843433d804639 22 XM80Cz+0SI2JglqWOboANKyzpc0 Fig. 3a, Extended Data Fig. 4a–c Removed from nuclear basket in bridge midzone during nuclear division ------- COMMENT: 21d843433d804639 23 XM80Cz+0SI2JglqWOboANKyzpc0 Fig. 3a, Extended Data Fig. 4a–c Removed from nuclear basket in bridge midzone during nuclear division ------- COMMENT: 21d843433d804639 25 yyMNf1x4ofpAA8TPiE7bhUbV4SU fig 3b ------- COMMENT: 21d843433d804639 26 iS9UIhn0U8wP+TfgG2Ko0a9ve/k (comment: causally upstream?) ------- COMMENT: 21d843433d804639 27 OqZYDPo2V04Oo3cyDxzK+OKGcC0 Instead, the repair process was associated with recruitment of the ESCRTIII protein Cmp7 (Fig. 4d) to sites of local NEB20 (Fig. 4d, Extended Data Fig. 8b, c). ------- COMMENT: 21d843433d804639 28 Z0Vgn0obNTR7YBcpJQWguTOzyQM lem2 (which encodes Lem2, the binding partner of Cmp7) is also SL with les1 ------- COMMENT: 21f56eb78da62621 1 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 21f56eb78da62621 2 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 21f56eb78da62621 3 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 21f56eb78da62621 4 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 21f56eb78da62621 5 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 21f56eb78da62621 6 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 21f56eb78da62621 7 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 21f56eb78da62621 8 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 21fef5a562927ba9 1 zSmRQjGTMTP8X6e0WNBITyTKqmo (comment: CONDITION 100 ug/ml canavanine) ------- COMMENT: 223397563bf5eae7 1 b8wiYfw2SAg9Du72gFPXakMgHf0 Fluorescence microscopy revealed that Cdk11 was expressed and mainly concentrated in the nucleus (Figure 1A). ------- COMMENT: 223397563bf5eae7 2 FwhKKhQNaTD+78k/3ZQ2Nwfqsso A tandem affinity purification (TAP) identified physical partners of Cdk11, including an unchar- acterized cyclin (SPAC1296.05c) that was confirmed to bind Cdk11 in independent coimmunoprecipitation experiments (Fig- ure 1C and 1D). ------- COMMENT: 223397563bf5eae7 3 FwhKKhQNaTD+78k/3ZQ2Nwfqsso A tandem affinity purification (TAP) identified physical partners of Cdk11, including an unchar- acterized cyclin (SPAC1296.05c) that was confirmed to bind Cdk11 in independent coimmunoprecipitation experiments (Fig- ure 1C and 1D). ------- COMMENT: 223397563bf5eae7 4 7wA1y93379H++7DkIu33Hg1zDHc in contrast to Mcs6 (the Cdk7 ortholog), which readily phosphor- ylated the GST-CTD fusion in vitro (Figure 1E) (Drogat and Her- mand, 2012) ------- COMMENT: 223397563bf5eae7 5 V/WOJQIcFs+ztd08iY7Z9amQV44 In addition, while the inactivation of the well- described CTD serine 5 or serine 2 kinases (Mcs6 [Cdk7] and Lsk1 [Cdk12], respectively) specifically decreased the phos- phorylation level of these two residues in vivo, the absence of cdk11 had no effect (Figure 1F). ------- COMMENT: 223397563bf5eae7 6 V/WOJQIcFs+ztd08iY7Z9amQV44 In addition, while the inactivation of the well- described CTD serine 5 or serine 2 kinases (Mcs6 [Cdk7] and Lsk1 [Cdk12], respectively) specifically decreased the phos- phorylation level of these two residues in vivo, the absence of cdk11 had no effect (Figure 1F). ------- COMMENT: 223397563bf5eae7 7 7wA1y93379H++7DkIu33Hg1zDHc in contrast to Mcs6 (the Cdk7 ortholog), which readily phosphor- ylated the GST-CTD fusion in vitro (Figure 1E) (Drogat and Her- mand, 2012) ------- COMMENT: 223397563bf5eae7 9 kSALPmTnjzpMkMx6Ou0W/V927qs Therefore, we found no evidence of Cdk11 being a genuine CTD kinase in fission yeast. ------- COMMENT: 223397563bf5eae7 10 kSALPmTnjzpMkMx6Ou0W/V927qs Therefore, we found no evidence of Cdk11 being a genuine CTD kinase in fission yeast. ------- COMMENT: 223397563bf5eae7 11 ccT4mc3cGKOF+iqlbiIZR3BQENY The previously reported connection between Cdk11 and tran- scription, together with its nuclear localization and copurifica- tion with transcription regulators (although at weak level) led us to test its chromatin association. Gene-specific chromatin immunoprecipitation (ChIP) experiments showed that Cdk11- hemagglutinin (HA) was enriched onto chromatin compared to an untagged control (data not shown), and a genome-wide ChIP-on-chip analysis showed a broad distribution of Cdk11- HA. ------- COMMENT: 223397563bf5eae7 12 5YknaYFagRHupqlQ8FEkR998Df4 This possibility was confirmed by global expression profiling, showing that only 55 genes were significantly affected in the absence of Cdk11. (.....and srb mediator association) showed that the absence of either cdk11 or cdk8 resulted in very similar defects (up- or downregulation) that were more pronounced in the cdk8 mutant (Figure 2C). Quantitative RT-PCR confirmed this effect on representative genes and showed, in addition, that the expression defects were not cumulated in the double cdk8 cdk11 mutant (Fig- ure 2D). ------- COMMENT: 223397563bf5eae7 21 kSALPmTnjzpMkMx6Ou0W/V927qs Therefore, we found no evidence of Cdk11 being a genuine CTD kinase in fission yeast. ------- COMMENT: 223397563bf5eae7 22 kSALPmTnjzpMkMx6Ou0W/V927qs Therefore, we found no evidence of Cdk11 being a genuine CTD kinase in fission yeast. ------- COMMENT: 223397563bf5eae7 25 NJYa3CQBlGC8lYdWGlCC05o4ogc Combining the large-scale phosphoproteome data set and sequence alignment (Figures S4B and S4C), we determined that putative phosphoacceptors and in vitro analysis demon- strated that Cdk11 phosphorylates Med4 on three residues (Fig- ure 3B: S115, S204, and S218 ------- COMMENT: 223397563bf5eae7 26 NJYa3CQBlGC8lYdWGlCC05o4ogc Combining the large-scale phosphoproteome data set and sequence alignment (Figures S4B and S4C), we determined that putative phosphoacceptors and in vitro analysis demon- strated that Cdk11 phosphorylates Med4 on three residues (Fig- ure 3B: S115, S204, and S218 ------- COMMENT: 223397563bf5eae7 41 /ic62saMswH1FVm6TWJuaVM6Zbw At the contrary, the interaction between the kinase module subunit Cdk8 and the head subunit Med27 was completely abrogated when Cdk11 was inactivated (Figure 4B, middle panel). This role of Cdk11 in Mediator integrity was likely mediated by phosphorylation of Med27 and Med4 on the sites identified above (Figures 3 and 4A), because the inter- action between Cdk8 and either the Med27 or Med4 phos- phorylation mutants was specifically lost (Figure 4B, right panel). In contrast, the phosphorylation mutants of Med27 and Med4 still interacted with the middle subunit Med7 (Fig- ure 4B, right panel). These data indicate that the association of the kinase submodule and the S-Mediator requires the phosphorylation of Med27 and Med4 by Cdk11. ------- COMMENT: 223397563bf5eae7 42 g/opqN6fbL3CydFnWRKqkNwJ8DM At the contrary, the interaction between the kinase module subunit Cdk8 and the head subunit Med27 was completely abrogated when Cdk11 was inactivated (Figure 4B, middle panel). This role of Cdk11 in Mediator integrity was likely mediated by phosphorylation of Med27 and Med4 on the sites identified above (Figures 3 and 4A), because the inter- action between Cdk8 and either the Med27 or Med4 phos- phorylation mutants was specifically lost (Figure 4B, right panel). In contrast, the phosphorylation mutants of Med27 and Med4 still interacted with the middle subunit Med7 (Fig- ure 4B, right panel). These data indicate that the association of the kinase submodule and the S-Mediator requires the phosphorylation of Med27 and Med4 by Cdk11. ------- COMMENT: 224bb9b28b5395ed 1 sVIMML/19rjPoMzlchxX+tzXrok Here we also tested whether Cox14 interacts with Shy1 in S. pombe. Among these assembly factors of complex IV, Mss51, Pet117, Sco1, Cox14 were identified in the Shy1-FLAG eluate (Fig. 4a and b), ------- COMMENT: 224bb9b28b5395ed 2 Y9hOZOIu8/AboeJYvgpC3zLPJ+Y Here, our results showed that Shy1 is physically linked to Rip1, the structural subunit of complex III, suggesting that Shy1 co-isolated with complexes III and IV of the mitochondrial respiratory chain. ------- COMMENT: 224bb9b28b5395ed 3 XFC22Pi3rZjKDgIa/ohNxm1YT2Q Our results showed that Shy1 physically interacts with complex IV structural subunits Cox5 and Cox6 (Fig. 4a). ------- COMMENT: 224bb9b28b5395ed 4 XFC22Pi3rZjKDgIa/ohNxm1YT2Q Our results showed that Shy1 physically interacts with complex IV structural subunits Cox5 and Cox6 (Fig. 4a). ------- COMMENT: 224bb9b28b5395ed 5 sVIMML/19rjPoMzlchxX+tzXrok Here we also tested whether Cox14 interacts with Shy1 in S. pombe. Among these assembly factors of complex IV, Mss51, Pet117, Sco1, Cox14 were identified in the Shy1-FLAG eluate (Fig. 4a and b), ------- COMMENT: 224bb9b28b5395ed 6 sVIMML/19rjPoMzlchxX+tzXrok Here we also tested whether Cox14 interacts with Shy1 in S. pombe. Among these assembly factors of complex IV, Mss51, Pet117, Sco1, Cox14 were identified in the Shy1-FLAG eluate (Fig. 4a and b), ------- COMMENT: 224bb9b28b5395ed 7 sVIMML/19rjPoMzlchxX+tzXrok Here we also tested whether Cox14 interacts with Shy1 in S. pombe. Among these assembly factors of complex IV, Mss51, Pet117, Sco1, Cox14 were identified in the Shy1-FLAG eluate (Fig. 4a and b), ------- COMMENT: 224bb9b28b5395ed 9 1JKpTPaO4KqINLp1o4F5NEDcvpY Our results showed that the levels of cob1, cox1, cox2, cox3, atp6, atp8 and atp9 RNAs were reduced in ∆shy1 cells (Fig. 5b). Furthermore, the abundance of mt-rRNAs (rns and rnl) was also reduced in shy1 deletion cells compared to WT cells, suggesting that deletion of shy1 affects the overall levels of the expression of the mtDNA-encoded genes. ------- COMMENT: 224bb9b28b5395ed 10 3LWKt75I8mI8G7nZyZFu87tmgxI As shown in Fig. 5c, the mtDNA copy number is slightly increased in ∆shy1 cells compared to that of in WT strain, indicating the observed reduction in mitochondrial RNA is not attributed to a decrease in mtDNA levels. ------- COMMENT: 224bb9b28b5395ed 11 hXu+86u4dn9Aygvs7+4Vwu3sNsA Our results showed that the expression of Cob1, Cox1, Cox2, Cox3, and Atp6 were greatly reduced in ∆shy1 cells (Fig. 5d). ------- COMMENT: 224bb9b28b5395ed 12 j+yYWZikT6TAR7SY2tK5uRjTMpw Collectively, our findings strongly indicate that Shy1 is affixed to the mitochondrial inner membrane./Fig. 6. Shy1 is localized in the mitochondrial inner membrane. ------- COMMENT: 224bb9b28b5395ed 13 DFh76onnuSdd3Jv/ar4V2RPmWGc The levels of supercomplexes III2IV2 and III2IV were found to be lower in ∆shy1 cells compared to WT cells, while the abundance of COA complexes increased (Fig. 7a), indicating that the formation of supercomplexes involving complex IV is influenced by deletion of shy1. In contrast, the abundance of III2 and V complexes was largely unchanged in ∆shy1 cells (Fig. 7a). ------- COMMENT: 224bb9b28b5395ed 15 Fna1SKRNeczZQw5cyV61KKFSKNk The cell growth of the ∆shy1 mutant exhibited a notable decline when cultivated in glycerol medium in comparison to the wild-type (WT) strain (Fig. 5a). ------- COMMENT: 224bb9b28b5395ed 16 d2bKAh5t2/EIXjNEvGmZRxJ7sGk Conversely, the growth reduction was marginal when cultured in glucose medium, which supports fermentative growth and thus necessitates moderate respiratory activity43. ------- COMMENT: 224bb9b28b5395ed 17 DFh76onnuSdd3Jv/ar4V2RPmWGc The levels of supercomplexes III2IV2 and III2IV were found to be lower in ∆shy1 cells compared to WT cells, while the abundance of COA complexes increased (Fig. 7a), indicating that the formation of supercomplexes involving complex IV is influenced by deletion of shy1. In contrast, the abundance of III2 and V complexes was largely unchanged in ∆shy1 cells (Fig. 7a). ------- COMMENT: 224bb9b28b5395ed 18 dQgidJAinTkRYA1V+FoJAc2Mc7U Our results revealed a slight decrease in complex III enzyme activity in ∆shy1 cells compared to WT cells (Supplementary Fig. S5 online), suggesting that shy1 affects complex III in ways other than its assembly. ------- COMMENT: 224fc1be175a11d5 1 khsisV/+a3EzkwQ6iLhnNm1HrpM (Fig. 6) ------- COMMENT: 224fc1be175a11d5 2 khsisV/+a3EzkwQ6iLhnNm1HrpM (Fig. 6) ------- COMMENT: 2278d0c4da671cf5 1 v9N2zCiMCf38uIWiaRdBBeVPFq0 Figure S3. mlo3∆ cells maintain H3K9me2 and Swi6 localization at centromeres, mating type locus and subtelomeric loci ------- COMMENT: 2278d0c4da671cf5 2 v9N2zCiMCf38uIWiaRdBBeVPFq0 Figure S3. mlo3∆ cells maintain H3K9me2 and Swi6 localization at centromeres, mating type locus and subtelomeric loci ------- COMMENT: 2278d0c4da671cf5 3 v9N2zCiMCf38uIWiaRdBBeVPFq0 Figure S3. mlo3∆ cells maintain H3K9me2 and Swi6 localization at centromeres, mating type locus and subtelomeric loci ------- COMMENT: 2278d0c4da671cf5 4 ttvaLTOcGv7x5jtJ/ffvQ0AwjII However, mlo3Δ resulted in a considerable increase in the levels of centromeric repeat transcripts, although to a lesser extent than in clr4Δ (Fig. 1B). ------- COMMENT: 2278d0c4da671cf5 5 ttvaLTOcGv7x5jtJ/ffvQ0AwjII However, mlo3Δ resulted in a considerable increase in the levels of centromeric repeat transcripts, although to a lesser extent than in clr4Δ (Fig. 1B). ------- COMMENT: 2278d0c4da671cf5 6 ttvaLTOcGv7x5jtJ/ffvQ0AwjII However, mlo3Δ resulted in a considerable increase in the levels of centromeric repeat transcripts, although to a lesser extent than in clr4Δ (Fig. 1B). ------- COMMENT: 2278d0c4da671cf5 7 ttvaLTOcGv7x5jtJ/ffvQ0AwjII However, mlo3Δ resulted in a considerable increase in the levels of centromeric repeat transcripts, although to a lesser extent than in clr4Δ (Fig. 1B). ------- COMMENT: 2278d0c4da671cf5 8 kIywSjvE7ErEo+nfjHP7/rLSG2A A yeast two-hybrid screen using full-length Clr4 as the bait identified Mlo3 (12) as an interacting protein (table S1). Mlo3 is related to Saccharomyces cerevisiae Yra1 and mammalian Aly/REF (13) and is required for nuclear export of RNA (13). Immunoprecipitation analysis detected Mlo3 interacting with Clr4 (Fig. 1A) and another ClrC subunit, Rik1 (fig. S1). Moreover, recombinant Mlo3 bound Clr4, and this interaction was mediated by the amino-terminal (amino acids 1 to 55) and carboxy-terminal (amino acids 134 to 199) regions of Mlo3 (fig. S2), known to bind mRNA export machinery (13). Thus, Clr4 associates with Mlo3 in vitro and in vivo. ------- COMMENT: 2278d0c4da671cf5 9 zgFIbPvpBO85wx3VADVSR87BqOw We found that Mlo3 coimmunoprecipitated with Chp1, a subunit of RITS (Fig. 1C). ------- COMMENT: 2278d0c4da671cf5 10 8r987dk7p7J7T8a/lhZ+uR2AWxw his interaction was not sensitive to DNase I and RNase A treatment but was severely compromised upon loss of Clr4 (Fig. 1C), suggesting that Clr4 connects Mlo3 to RNA interference (RNAi). ------- COMMENT: 2278d0c4da671cf5 11 ILVXrDOUyys4LsHTw1XkC2POIwU Indeed, mlo3Δ caused severe reduction in the levels of centromeric siRNAs (Fig. 1D). ------- COMMENT: 2278d0c4da671cf5 12 Mv+kY4hZK0NP7GWnxv1+kUAbLZ8 (comment: vw: I did not use processing because this seems to be catabolism) Thus, in addition to creating H3K9me binding sites for RITS, Clr4 physically and functionally links RITS to Mlo3 to mediate processing of centromeric transcripts. ------- COMMENT: 2278d0c4da671cf5 13 oCD6n+GlWWYf/KEggBl8N9xgfto Recombinant Clr4 could methylate the carboxy- terminal region of Mlo3, but not the amino-terminal or middle region (Fig. 2A). Within the carboxy-terminal region, lysines 165 and 167 are in a sequence context that resembles H3K9. We mutated these and lysines 179 and 180 to alanine. A methylation assay using recombinant Mlo3 carrying single- or double-mutant combinations showed that Clr4 methylates K167 of Mlo3 in vitro (Fig. 2B). ------- COMMENT: 2278d0c4da671cf5 14 P7JJaZByUPKGCr2aHUh+0lnc4rU A methylation assay using recombinant Mlo3 carrying single- or double-mutant combinations showed that Clr4 methylates K167 of Mlo3 in vitro (Fig. 2B). ------- COMMENT: 2278d0c4da671cf5 15 P7JJaZByUPKGCr2aHUh+0lnc4rU A methylation assay using recombinant Mlo3 carrying single- or double-mutant combinations showed that Clr4 methylates K167 of Mlo3 in vitro (Fig. 2B). ------- COMMENT: 2278d0c4da671cf5 16 jr6lUIqP9nL+3CkSRrKqNK/Rj6Y Interestingly, mlo3-A caused a decrease in levels of centromeric siRNA as compared to WT (Fig. 2D). ------- COMMENT: 2278d0c4da671cf5 17 r8a7zBjBz95fJ6F5Zg4PuSbY1ds Further reduction in siRNAs was observed in mlo3-A H3K9R double mutant (Fig. 2D), although a residual signal seemed to be present when compared to clr4Δ. ------- COMMENT: 2278d0c4da671cf5 18 Yu6iFbRykZMn2rdbrOWSR3/Rcn8 Mlo3 showed a broad distribution at euchromatic loci and a relative depletion at heterochromatic regions (figs. S5 and S6). ------- COMMENT: 2278d0c4da671cf5 19 2Q8qmKDg+F9JY96w5jADXXLITEU Because clr4Δ and ago1Δ enhance antisense RNA levels when combined with a variant histone h2a.zΔ (10), we examined the mlo3-A mutant transcriptome with or without H2A.Z. Like clr4Δ and ago1Δ, mlo3-A also showed weak up- regulation of antisense RNAs (4.7% genes) (fig. S9). ------- COMMENT: 2278d0c4da671cf5 21 DbQVNtGxNDb1Tg5/umNKEy4zAcM Expression profiling of mlo3Δ cells on both DNA strands showed dramatic accumulation of antisense RNAs at euchromatic loci (~23.5% of genes) (fig. S7), in particular at convergent genes (fig. S8) ------- COMMENT: 2278d0c4da671cf5 22 iyvZpilWTnfcRsOVshT3n8Irzbg However, the mlo3-A h2a.zΔ double mutant showed a synergistic increase in antisense RNAs (18.6% of genes) (Fig. 3C and figs. S8 and S9)Because clr4Δ and ago1Δ enhance antisense RNA levels when combined with a variant histone h2a.zΔ (10), we examined the mlo3-A mutant transcriptome with or without H2A.Z. Like clr4Δ and ago1Δ, mlo3-A also showed weak up- regulation of antisense RNAs (4.7% genes) (fig. S9). ------- COMMENT: 2278d0c4da671cf5 23 eRDsPNvy071ixxKZ32fazxvPKS4 Mlo3 is required for suppression of antisense RNAs targeted by Clr4 and by the exosome. (A) Strand-specific RTPCR of RNA isolated from WT and mlo3Δ ------- COMMENT: 2278d0c4da671cf5 24 eRDsPNvy071ixxKZ32fazxvPKS4 Mlo3 is required for suppression of antisense RNAs targeted by Clr4 and by the exosome. (A) Strand-specific RTPCR of RNA isolated from WT and mlo3Δ ------- COMMENT: 2278d0c4da671cf5 25 eRDsPNvy071ixxKZ32fazxvPKS4 Mlo3 is required for suppression of antisense RNAs targeted by Clr4 and by the exosome. (A) Strand-specific RTPCR of RNA isolated from WT and mlo3Δ ------- COMMENT: 2278d0c4da671cf5 27 OsArbvSsyX1CNJcXoj5O/CWMR1I Thus, Mlo3 physically associates with TRAMP, a complex involved in the surveillance and degradation of aberrant RNA by the exosome. In this regard, the antisense profile of mlo3Δ closely resembles that of rrp6Δ (Fig. 3, C and E, and fig. S8). ------- COMMENT: 2306fd737a53d0b4 1 /K45/UoNdFYSVk5+S4hFH36xNEw (comment: I specifically used that term name because I did not want to discriminate whether Cuf2 is a negative or a positive regulator of transcription, even though in the paper we have put emphasis on the fact that meiotic genes are up-regulated in the absence of Cuf2 (so that Cuf2 would be a repressor). The reason is that there are also many other genes that are down-regulated in the cuf2delta mutant compare to WT. We still don't know which effect is direct and/or indirect. Thus, we don't want to exclude that Cuf2 might act as an activator, a repressor or both, for now. ------- COMMENT: 2306fd737a53d0b4 28 /K45/UoNdFYSVk5+S4hFH36xNEw (comment: I specifically used that term name because I did not want to discriminate whether Cuf2 is a negative or a positive regulator of transcription, even though in the paper we have put emphasis on the fact that meiotic genes are up-regulated in the absence of Cuf2 (so that Cuf2 would be a repressor). The reason is that there are also many other genes that are down-regulated in the cuf2delta mutant compare to WT. We still don't know which effect is direct and/or indirect. Thus, we don't want to exclude that Cuf2 might act as an activator, a repressor or both, for now.) ------- COMMENT: 231f5f6efb2fbea9 8 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 238851c4baaed103 20 2xemWIuzBn5Yk1L4sA7k3tHlIk8 (comment: Ser-2 of the heptad repeat) ------- COMMENT: 238989fa4ca1c956 1 Ppgtb1Y2uexgU/A3dscyuG/n4ZA (comment: The fission yeast S. pombe has an HP1 homologue Swi6, which contains a chromo domain that is closely related to those of HP1 family members.) Figure 4a shows that the Swi6 chromo domain is able to bind H3 peptide methylated at Lys 9, whereas its chromo- shadow domain has no methyl-binding activity. ------- COMMENT: 238989fa4ca1c956 5 h7N07LliMmTNO0b6e2VYKvbUpr8 whereas the Clr4-G341D strain shows loss of localiza- tion from the nuclear periphery and accumulation of more diffuse staining over the nucleolus (Fig. 4c). ------- COMMENT: 238989fa4ca1c956 6 7q307+bNHktRuTYfCXWre9YypYc loss of association of Swi6 with centromeres should result in expression of a normally silent marker gene embedded in centro- meric chromatin. Figure 4e shows that this is indeed the case. On indicator plates, wild-type strains silence the centromeric ade6+ marker, which results in red, repressed colonies13; however, in strains lacking Clr4 (D) or strains defective in Clr4 methylase activity (G341D), this ade6+ gene is clearly expressed, resulting in the formation of white colonies. ------- COMMENT: 238989fa4ca1c956 7 7q307+bNHktRuTYfCXWre9YypYc loss of association of Swi6 with centromeres should result in expression of a normally silent marker gene embedded in centro- meric chromatin. Figure 4e shows that this is indeed the case. On indicator plates, wild-type strains silence the centromeric ade6+ marker, which results in red, repressed colonies13; however, in strains lacking Clr4 (D) or strains defective in Clr4 methylase activity (G341D), this ade6+ gene is clearly expressed, resulting in the formation of white colonies. ------- COMMENT: 23bb4638a11a6ec8 26 0HRunq8Gfr20S8vY4qdlMMPy8S4 (comment: same as rad51delta alone) ------- COMMENT: 23bb4638a11a6ec8 27 0HRunq8Gfr20S8vY4qdlMMPy8S4 (comment: same as rad51delta alone) ------- COMMENT: 23c5a42491be246e 1 lhQiH79qE+VU1FeoSPxM8VhE5XM Among 24 genes proposed to be MBF targets (Figure 1A) [14], the promoters of 19 genes were substantially enriched in the Yox1p ChIPs, including yox1 itself (Figure 1B). ------- COMMENT: 23c5a42491be246e 2 VhrSMJ2QDPBiOShxO+ah+3Pq3Fs MBF, we performed ChIP-chip using an antibody against the MBF component Cdc10p. As for Yox1p, among the 24 proposed MBF targets, the promoters of the same 19 genes, including cdc10 itself and yox1, were substantially enriched in the Cdc10p ChIPs (Figure 1C); ------- COMMENT: 23c5a42491be246e 3 fvjSkJa57GRJ8emCfdzQJV91Nnk Taken together, these data show that yox1 is transcriptionally activated by MBF, and Yox1p in turn binds itself to MBF target genes. ------- COMMENT: 23c5a42491be246e 4 rnb876DM0K45KzNE/5srVYIyPkw HA-tagged Yox1p revealed an interaction between Cdc10p and Yox1p (Figure 2A). Moreover, anti-myc immuno complexes prepared from cells expressing Res2p-myc, Yox1p-HA or both showed an interaction between Res2p and Yox1p (Figure 2B) These results are confirmed by independent data from a recent mass spectrometry-based analysis of affinity-purified Res2p and Nrm1p complexes [20]. Based on these mass spectrometry data, Yox1p interacts with both Res2p and Nrm1p, with coverage of Yox1p by specific peptides being similar to the MBF component Cdc10p (data not shown). Together, these data indicate that Yox1p physically associates with the MBF complex and thus represents a new component of MBF. ------- COMMENT: 23c5a42491be246e 5 LXpFzoNujYhJ5nV219NAlv+05m0 This analysis revealed that Yox1p binding to the cdc22 promoter depends on both of the MBF components tested, Res2p and Nrm1p (Figure 2C). We conclude that Yox1p can bind to MBF- regulated promoters only via intact MBF. ------- COMMENT: 23c5a42491be246e 6 LXpFzoNujYhJ5nV219NAlv+05m0 This analysis revealed that Yox1p binding to the cdc22 promoter depends on both of the MBF components tested, Res2p and Nrm1p (Figure 2C). We conclude that Yox1p can bind to MBF- regulated promoters only via intact MBF. ------- COMMENT: 23c5a42491be246e 7 FikvEoqp9nUxRuyjsUP7Qa5jhL0 the Yox1p levels were low during M/G1-phase but then strongly increased during S-phase (Figure 2D, top), peaking about 40 minutes after the peak of yox1 mRNA levels. ------- COMMENT: 23c5a42491be246e 8 UF9/cav6mULn5/Rel+DfoqaC7E8 The cell cycle-regulated target genes bound by both Yox1p and Cdc10p tended to be more highly expressed in yox1D than in wild-type cells (Figure 3A,B). In yox1D cells, on the other hand, the Yox1p/Cdc10p targets showed little or no cell-cycle regulation, whereas the cell-cycle regulation of Ace2p targets was not affected (Figure 4A). ------- COMMENT: 23c5a42491be246e 9 AV8ss5jZjEmoGkDKww6QX05LP74 kinesin-like protein, which was bound by both Yox1p and Cdc10p and induced in yox1D cells, suggesting that klp8 is regulated by both Ace2p and MBF. ------- COMMENT: 23c5a42491be246e 11 sH7hqxulkFBGtDmQ65eq1Eq4m0E The Yox1p/Cdc10p target genes showed consistently higher Pol II occupancy in yox1D than in wild- type cells (Figure 3C,D), ------- COMMENT: 23c5a42491be246e 12 gyqjnUshmfkB9AUk6ToilyI94wY A few genes, however, seem to be positively regulated by Yox1p (Figure 3A,B). An example is mfm2 (Figure 3A), encoding a precursor for the M-factor peptide (a mating pheromone) [33], and the neighbouring SPAC513.04, a sequence orphan that is divergently transcribed from mfm2. ------- COMMENT: 23c5a42491be246e 13 gyqjnUshmfkB9AUk6ToilyI94wY A few genes, however, seem to be positively regulated by Yox1p (Figure 3A,B). An example is mfm2 (Figure 3A), encoding a precursor for the M-factor peptide (a mating pheromone) [33], and the neighbouring SPAC513.04, a sequence orphan that is divergently transcribed from mfm2. ------- COMMENT: 23c5a42491be246e 14 yEGvfWKPfVsd6OmAIeT90/U691s Another example is map1, encoding a MADS-box transcription factor involved in the transcriptional response during mating ------- COMMENT: 23c5a42491be246e 15 ZxMRyMnRnyBk1fAkLUOVoXGmGrw Taken together, we conclude that cell-cycle regulated transcription of Yox1p/Cdc10p target genes is highly deficient in yox1D cells, reflecting that these genes are no longer down-regulated after S-phase. ------- COMMENT: 23c5a42491be246e 16 ZxMRyMnRnyBk1fAkLUOVoXGmGrw Taken together, we conclude that cell-cycle regulated transcription of Yox1p/Cdc10p target genes is highly deficient in yox1D cells, reflecting that these genes are no longer down-regulated after S-phase. ------- COMMENT: 23c5a42491be246e 17 IGzluJ2tD6u7RiopBiKdfUelR3Q yox1D cells were viable and did not show any overall growth defect (Figure 4B). ------- COMMENT: 23c5a42491be246e 18 oCpqJ2i5bjgsQrDMt4qiS8eBBNU The septation index was marginally higher in yox1D compared to wild-type cells (10.4% vs 9.4%), suggesting a slight delay in cytokinesis and/or cell separation. ------- COMMENT: 23c5a42491be246e 19 pLQRhcqA20FoP9kPcPm7oDIHc3k The yox1D cells were about 14% longer on average than wild-type cells during septation (17.4 mm vs 15.3 mm) (Figure 4C). ------- COMMENT: 23c5a42491be246e 20 LGxQ/epAgXqtAC15k+qu6awTh7s The promoters of 76 genes were substantially and consistently enriched in both Cdc10p and Yox1p ChIPs, including the 19 previously discussed MBF target genes (Fig 5A,B; Table S3). ------- COMMENT: 23dda2714be075ae 1 kX5qDYQ2OkHGRWWsFcEcxFH0plY fig 8a ------- COMMENT: 23dda2714be075ae 2 kX5qDYQ2OkHGRWWsFcEcxFH0plY fig 8a ------- COMMENT: 23dda2714be075ae 3 GAqy8t8jp/JwHdUGBgIUhMOswPI fig 8a ------- COMMENT: 23dda2714be075ae 4 H3pHjd4y03RbmzbXiL1fILnS/Rk fig 8d ------- COMMENT: 23dda2714be075ae 5 J88iMmtD1vy5e0yMPMB5BOF2oog fig 8d ------- COMMENT: 23dda2714be075ae 6 PEOc8XnaSgImeFg6EFjyhCfH+Ho Figure 8e ------- COMMENT: 23dda2714be075ae 10 VQwyyMBfQueRCgWSq5NNqBnXO6Q Figure 5D ------- COMMENT: 23dda2714be075ae 14 cC1gseHqq1dFJtnucZ320TAVmeE (comment: GFP-LactC2 probe expressed from pREP3X) ------- COMMENT: 23dda2714be075ae 15 kJjt5poJqZZQ8oPC2zgIdsZKbz8 fig 4B. (comment: GFP-LactC2 probe expressed from pREP3X) ------- COMMENT: 23dda2714be075ae 16 cC1gseHqq1dFJtnucZ320TAVmeE (comment: GFP-LactC2 probe expressed from pREP3X) ------- COMMENT: 23dda2714be075ae 17 AuzP/M+VVRWwFeJyxNQIQkgtDrU fig 4 (comment: GFP-LactC2 probe expressed from pREP3X) ------- COMMENT: 23dda2714be075ae 18 cC1gseHqq1dFJtnucZ320TAVmeE (comment: GFP-LactC2 probe expressed from pREP3X) ------- COMMENT: 23dda2714be075ae 19 cC1gseHqq1dFJtnucZ320TAVmeE (comment: GFP-LactC2 probe expressed from pREP3X) ------- COMMENT: 23dda2714be075ae 22 XCBormE8rdLyTS6bWALFCM6x7ww fig 4 (comment: GFP-LactC2 probe expressed from pREP3X) ------- COMMENT: 23dda2714be075ae 23 lmDHyQ9cb+UOD7KOeLCtjGbs6tk Figure 3, A and E ------- COMMENT: 23dda2714be075ae 24 lmDHyQ9cb+UOD7KOeLCtjGbs6tk Figure 3, A and E ------- COMMENT: 23dda2714be075ae 25 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: 23dda2714be075ae 26 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: 23ea57b8910e06c0 1 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 2 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 3 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 4 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 5 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 6 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 7 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 8 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 9 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 10 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 11 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 12 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 13 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 14 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 15 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 16 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 17 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 18 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 19 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 20 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 21 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 22 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 23 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 24 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 25 4tRJYDFAQUhOsjRn2AiZhlVPx/I Table 1 The nuclear envelope for all the mutants analysed is marked with Ish1-yEGFP integrated in the gene deletion locus. The the NC ratio of the control is reduced from 0.08 to 0.05. ------- COMMENT: 23ea57b8910e06c0 26 yOIdk/1Stn1diJWSBWaNDkFuPBc Fig6 The nuclear envelope is marked with Cut11-GFP ------- COMMENT: 23ea57b8910e06c0 27 yOIdk/1Stn1diJWSBWaNDkFuPBc Fig6 The nuclear envelope is marked with Cut11-GFP ------- COMMENT: 23ea57b8910e06c0 28 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 23ea57b8910e06c0 29 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 23ea57b8910e06c0 30 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 23ea57b8910e06c0 31 yOIdk/1Stn1diJWSBWaNDkFuPBc Fig6 The nuclear envelope is marked with Cut11-GFP ------- COMMENT: 23eb2cb1b340e956 1 mzfrfnB4QwPQidEb+oTsWOj0V70 Although cwf10 2–135 aligns with snu114N (Fig. 1B), the S. pombe allele did not support growth at either 25°C or 32°C when integrated at the endogenous locus (data not shown), while the S. cerevisiae allele is viable at temperatures below 40°C (30). ------- COMMENT: 23eb2cb1b340e956 2 cKG3GHevIec7rgzSp2aIga9ys3M The cwf10-NTE strain formed slightly smaller colonies on solid media at all temperatures tested (Fig. 2A) and exhibited slow growth in liquid culture at 25°C and 36°C compared with wild-type cells (Fig. 2B and data not shown) ------- COMMENT: 23eb2cb1b340e956 3 cKG3GHevIec7rgzSp2aIga9ys3M The cwf10-NTE strain formed slightly smaller colonies on solid media at all temperatures tested (Fig. 2A) and exhibited slow growth in liquid culture at 25°C and 36°C compared with wild-type cells (Fig. 2B and data not shown) ------- COMMENT: 23eb2cb1b340e956 4 gTJQpecbPuYU6RbhtXWhG7ho3lg GENERAL SPLICING DEFECT RT-PCR analysis of tbp1_a, an mRNA intron highly sensitive to splicing defects (53), indicated that cwf10-NTE cells grown at 25°C accumulate unspliced tran- script (Fig. 2C). retrotransposon silencing by mRNA destabilization ------- COMMENT: 23eb2cb1b340e956 5 ZXELjVoqdSH6ETIcEiqEvA+7aCE Using quantitative Western blotting, we found that levels of Cwf10 were consistently higher in cwf10-NTE cells than in wild-type cells when quantified against the loading control Cdc2 (Cdk1) (Fig. 2I) ------- COMMENT: 23eb2cb1b340e956 6 c0hOf6tibphnPaDwQOZndYxNWZk In the cwf10-NTE background, levels of Brr2 and Prp3 were similar to levels seen in wild-type cells; however, both Cdc5 and Prp1 levels were reduced to 70% and 65%, respectively (Fig. 2I). ------- COMMENT: 23eb2cb1b340e956 7 c0hOf6tibphnPaDwQOZndYxNWZk In the cwf10-NTE background, levels of Brr2 and Prp3 were similar to levels seen in wild-type cells; however, both Cdc5 and Prp1 levels were reduced to 70% and 65%, respectively (Fig. 2I). ------- COMMENT: 23eb2cb1b340e956 8 c0hOf6tibphnPaDwQOZndYxNWZk In the cwf10-NTE background, levels of Brr2 and Prp3 were similar to levels seen in wild-type cells; however, both Cdc5 and Prp1 levels were reduced to 70% and 65%, respectively (Fig. 2I). ------- COMMENT: 23eb2cb1b340e956 9 c0hOf6tibphnPaDwQOZndYxNWZk In the cwf10-NTE background, levels of Brr2 and Prp3 were similar to levels seen in wild-type cells; however, both Cdc5 and Prp1 levels were reduced to 70% and 65%, respectively (Fig. 2I). ------- COMMENT: 23eb2cb1b340e956 10 i9KkxJSlMKNKfKM76UKCZ2EGlHw 1,193 introns (of 5,361 possible introns) whose splicing efficiency was significantly compromised in the mutant. ------- COMMENT: 23eb2cb1b340e956 12 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 13 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 14 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 15 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 16 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 17 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 18 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 19 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 20 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 21 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 22 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 23 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 24 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 25 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 26 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 27 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 28 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 29 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 30 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 31 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 32 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 33 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 34 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 35 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 36 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 37 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 38 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 39 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 40 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 41 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 42 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 43 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 44 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 45 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 46 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 47 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 48 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 49 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 50 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 51 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 52 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 53 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 54 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 55 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 56 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 57 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 58 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 59 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 60 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 61 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 62 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 63 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 64 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 65 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 66 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 67 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 68 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 69 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 70 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 71 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 72 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 73 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 74 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 23eb2cb1b340e956 75 Ejurv6XfASR/kPkT07QUZd3p378 (comment: GENERAL SPLICING DEFECT) The ratio of mature to premature signal for the tbp1_a intron was almost identical for the two truncations (2.1  0.2 for cwf10-NTE ver- sus 2.0  0.3 for cwf10 2–23), while the ratio in the wild-type strain was 9.7  1.5 (Fig. 6H). Th ------- COMMENT: 24045a664a77f653 2 rPzNcyGOKG4DqzGMVWE3qBCeOGs (comment: also inferrable (IC) from GO:0051787) ------- COMMENT: 2415808f7b13c5d0 25 Os7rFVj2scncbdeN4C4DkR6kay4 (comment: same as brc1delta alone; brc1 epistatic) ------- COMMENT: 2415808f7b13c5d0 63 Os7rFVj2scncbdeN4C4DkR6kay4 (comment: same as brc1delta alone; brc1 epistatic) ------- COMMENT: 2415808f7b13c5d0 64 Os7rFVj2scncbdeN4C4DkR6kay4 (comment: same as brc1delta alone; brc1 epistatic ------- COMMENT: 2415808f7b13c5d0 65 Os7rFVj2scncbdeN4C4DkR6kay4 (comment: same as brc1delta alone; brc1 epistatic) ------- COMMENT: 2415808f7b13c5d0 66 Os7rFVj2scncbdeN4C4DkR6kay4 (comment: same as brc1delta alone; brc1 epistatic) ------- COMMENT: 2415808f7b13c5d0 71 VWVnqnCABoHE5+9UVqW8hG4CuqM (comment: same as mus81delta alone; mus81 epistatic) ------- COMMENT: 2415808f7b13c5d0 72 VWVnqnCABoHE5+9UVqW8hG4CuqM (comment: same as mus81delta alone; mus81 epistatic) ------- COMMENT: 2415808f7b13c5d0 73 VWVnqnCABoHE5+9UVqW8hG4CuqM (comment: same as mus81delta alone; mus81 epistatic) ------- COMMENT: 2415808f7b13c5d0 74 VWVnqnCABoHE5+9UVqW8hG4CuqM (comment: same as mus81delta alone; mus81 epistatic) ------- COMMENT: 2415808f7b13c5d0 75 VWVnqnCABoHE5+9UVqW8hG4CuqM (comment: same as mus81delta alone; mus81 epistatic) ------- COMMENT: 2415808f7b13c5d0 76 VWVnqnCABoHE5+9UVqW8hG4CuqM (comment: same as mus81delta alone; mus81 epistatic) ------- COMMENT: 2415808f7b13c5d0 77 VWVnqnCABoHE5+9UVqW8hG4CuqM (comment: same as mus81delta alone; mus81 epistatic) ------- COMMENT: 2415808f7b13c5d0 78 VWVnqnCABoHE5+9UVqW8hG4CuqM (comment: same as mus81delta alone; mus81 epistatic) ------- COMMENT: 2415808f7b13c5d0 79 VWVnqnCABoHE5+9UVqW8hG4CuqM same as mus81delta alone; mus81 epistatic ------- COMMENT: 2415808f7b13c5d0 80 VWVnqnCABoHE5+9UVqW8hG4CuqM (comment: same as mus81delta alone; mus81 epistatic) ------- COMMENT: 2415808f7b13c5d0 81 VWVnqnCABoHE5+9UVqW8hG4CuqM (comment: same as mus81delta alone; mus81 epistatic) ------- COMMENT: 2415808f7b13c5d0 82 VWVnqnCABoHE5+9UVqW8hG4CuqM (comment: same as mus81delta alone; mus81 epistatic) ------- COMMENT: 2415808f7b13c5d0 83 VWVnqnCABoHE5+9UVqW8hG4CuqM (comment: same as mus81delta alone; mus81 epistatic) ------- COMMENT: 2415808f7b13c5d0 84 VWVnqnCABoHE5+9UVqW8hG4CuqM (comment: same as mus81delta alone; mus81 epistatic) ------- COMMENT: 2415808f7b13c5d0 85 VWVnqnCABoHE5+9UVqW8hG4CuqM (comment: same as mus81delta alone; mus81 epistatic) ------- COMMENT: 2415808f7b13c5d0 86 VWVnqnCABoHE5+9UVqW8hG4CuqM (comment: same as mus81delta alone; mus81 epistatic) ------- COMMENT: 2415808f7b13c5d0 91 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment: same as either single mutant) ------- COMMENT: 2415808f7b13c5d0 93 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment: same as either single mutant) ------- COMMENT: 2415808f7b13c5d0 94 Os7rFVj2scncbdeN4C4DkR6kay4 (comment: same as brc1delta alone; brc1 epistatic) ------- COMMENT: 2415808f7b13c5d0 95 Os7rFVj2scncbdeN4C4DkR6kay4 (comment: same as brc1delta alone; brc1 epistatic) ------- COMMENT: 2415808f7b13c5d0 102 Os7rFVj2scncbdeN4C4DkR6kay4 (comment: same as brc1delta alone; brc1 epistatic) ------- COMMENT: 2415808f7b13c5d0 103 Os7rFVj2scncbdeN4C4DkR6kay4 (comment: same as brc1delta alone; brc1 epistatic) ------- COMMENT: 2448c15f14dc6041 15 L+6JPSCedH6S06/2gWYG1xQMvnc (comment: same as cdc2delta alone) ------- COMMENT: 2448c15f14dc6041 26 WYfBMOdxs6T/28PCZP2OviB8m2A (comment: not shown it is ser/thr kinase activity) ------- COMMENT: 2467c1608e001a46 1 qx3KoJASn8VZ2d7j/d0agErIQ80 Fig 1A and C (comment: cdc18 transcription is not dependent on cdc2 function) ------- COMMENT: 2467c1608e001a46 2 iHusPpyRjbgECK29ZsfkgSYl/no Fig 1 B (comment: cdc2-M26 has no detectable kinase activity in G1 at restrictive temperature) ------- COMMENT: 2467c1608e001a46 3 5BJlgcNb0nhoPcW8lBo0WN702jg Fig 1D (comment: cdc2-M26 does not enter S phase even though cdc18 transcription is presence) ------- COMMENT: 2467c1608e001a46 4 /cln0/8SWzp8VsaxQzjbRXB7qeM Fig2 B ------- COMMENT: 2467c1608e001a46 5 /cln0/8SWzp8VsaxQzjbRXB7qeM Fig2 B ------- COMMENT: 2467c1608e001a46 6 x+kWu6JM2BGBaDYkeTlDoN1JefM Fig2 B cells do not undergo re replication at restrictive temperature ------- COMMENT: 2467c1608e001a46 7 1BoNKSoOC+46oykUSgiuyIFL2RQ Fig2A cells do not undergo re replication at restrictive temperature but cdc18 transcript increases ------- COMMENT: 2467c1608e001a46 8 /cln0/8SWzp8VsaxQzjbRXB7qeM Fig2 B ------- COMMENT: 2467c1608e001a46 9 MiyBDw+UYYNLjhSBpS8q33DMeJ0 Fig3A cdc18 transcript accumulates in absence of cig1, cig2 and cdc13 ------- COMMENT: 2467c1608e001a46 10 qLCNXx9Q/saxnsjMFVLWTGa+vD0 Fig3A cdc18 transcript accumulates in absence of cdc13 ------- COMMENT: 2467c1608e001a46 11 yaA0RHFfn7BMoAX13AS/hZMNv3g Fig3B cdc18 protein accumulates in absence of cig1, cig2 and cdc13 ------- COMMENT: 2467c1608e001a46 12 AseIGJjFTWzHPyC1LGAOezohtRU Fig3C no DNA replication in absence of all 3 cyclins ------- COMMENT: 2467c1608e001a46 13 ME1ipPNmXJLsejkWAkxZa2oGP4g Fig3C DNA replication in presence of cig1 and cig2 ------- COMMENT: 2467c1608e001a46 14 jynKJuwS6ZFNpMphXki4zOh9uXk Fig3C Abnormal DNA replication with cut DNA replication in absence cig1, cig2 and cdc13 promoter ON some cells have a cut phenotype. (comment: NOT sure the data warrants an annotation) ------- COMMENT: 2467c1608e001a46 15 mqrJibkMUW6BxQDl7Q16TSB/ZYs Fig3C, D Absence of cdc2 kinase activity in absence cig1, cig2 and cdc13 ------- COMMENT: 2467c1608e001a46 16 Y4tyerY+dHZfl+/D+grsAI3ogCg Fig3C, D cdc13 promoter ON cdc2 kinase activity acts after the accumulation of cdc18 protein to bring about the G1/S transition ------- COMMENT: 2467c1608e001a46 20 4iT05obqbpOH5wHpKyQpbIID05o Fig 4C Cdc2 not required for active cdc10 dependent transcription during S phase ------- COMMENT: 2467c1608e001a46 21 hpgM1NTPr7dnaoCQuJ4OENIpWyQ Fig 4C Cdc18 transcript is low during G2 ------- COMMENT: 2467c1608e001a46 22 hpgM1NTPr7dnaoCQuJ4OENIpWyQ Fig 4C Cdc18 transcript is low during G2 ------- COMMENT: 2467c1608e001a46 23 D6GrYMjsxsdxL8Jn+E+etvEy+3A Cdc2 kinase activity is low during G2 data not shown ------- COMMENT: 2467c1608e001a46 25 urOpmJ7V2cOTeJDX6yi2ZCNyVFA Fig 4D ------- COMMENT: 2467c1608e001a46 26 urOpmJ7V2cOTeJDX6yi2ZCNyVFA Fig 4D ------- COMMENT: 2467c1608e001a46 27 uB5yzNCd2A2qXAYAggzeaRhf0UM Fig5A, B decreased cdc18 transcript in HU block and on release ------- COMMENT: 2467c1608e001a46 28 x8EDkJZPg3WBck9XGGzePIEQWs0 Fig5A decreased cdc18 transcript in HU block and on release ------- COMMENT: 2467c1608e001a46 29 uB5yzNCd2A2qXAYAggzeaRhf0UM Fig5A, B decreased cdc18 transcript in HU block and on release ------- COMMENT: 2467c1608e001a46 30 nalf8x+0M8J6H8gnfiK4k93OKf8 Fig5A, B level of cdc18 transcript does not decreased after release from HU block ------- COMMENT: 2467c1608e001a46 31 wA2zDi2ljZyRdZxkVyg7LW8joIY Fig 5C ------- COMMENT: 2467c1608e001a46 32 iIzNHoEuw86c0uRrGTYtciItrHI Fig5C rep2delta has no effect on cdc18 transcript levels in the absence of res2 ------- COMMENT: 2467c1608e001a46 34 OXqCw37/Uyk9HcquOWmbm+UAQCU Fig6C ------- COMMENT: 2467c1608e001a46 35 OXqCw37/Uyk9HcquOWmbm+UAQCU Fig6C ------- COMMENT: 2467c1608e001a46 36 M9juvCneX0OJZCB2t0ZBjwYHP+4 Fig6D ------- COMMENT: 2467c1608e001a46 37 M9juvCneX0OJZCB2t0ZBjwYHP+4 Fig6D ------- COMMENT: 2467c1608e001a46 38 grAd6B7l6k+U9Mt0sU9mRy+4F+8 Fig6B ------- COMMENT: 2467c1608e001a46 39 grAd6B7l6k+U9Mt0sU9mRy+4F+8 Fig6B ------- COMMENT: 2467c1608e001a46 40 CQGtsMsb1Fz3rI4URnEB3BTVeqI Data not shown ------- COMMENT: 2467c1608e001a46 41 qCCn1sdHNnQ0O39sczU96cJ7qGY Fig7 (comment: CHECK res1 on multi copy pREP3X ON) ------- COMMENT: 2467c1608e001a46 42 qCCn1sdHNnQ0O39sczU96cJ7qGY Fig7 (comment: CHECK res1 on multi copy pREP3X ON) ------- COMMENT: 2467c1608e001a46 43 qCCn1sdHNnQ0O39sczU96cJ7qGY Fig7 (comment: CHECK res1 on multi copy pREP3X ON) ------- COMMENT: 2467c1608e001a46 44 1HG7HkAze7m1hZX5NVXtHu9jutE Fig7 ------- COMMENT: 2467c1608e001a46 45 g4vTGlDw6WfTX+44+aW5oXyxdcw Fig7 (comment: CHECK res2 on multi copy pREP3X ON) ------- COMMENT: 2467c1608e001a46 46 g4vTGlDw6WfTX+44+aW5oXyxdcw Fig7 (comment: CHECK res2 on multi copy pREP3X ON) ------- COMMENT: 2467c1608e001a46 47 tm7aY2inAo+REoSWlt3OVBpegLM Fig8 (comment: DSC1 is now called MBF) ------- COMMENT: 2467c1608e001a46 48 9sQn9d03NsqPRghIX5XjZV43sVs Fig8B (comment: DSC1 is now called MBF) ------- COMMENT: 2467c1608e001a46 49 tm7aY2inAo+REoSWlt3OVBpegLM Fig8 (comment: DSC1 is now called MBF) ------- COMMENT: 2467c1608e001a46 50 9sQn9d03NsqPRghIX5XjZV43sVs Fig8B (comment: DSC1 is now called MBF) ------- COMMENT: 2467c1608e001a46 51 ERvJnMOJ0oXZ1FVbeSp/q9F9hPU Fig 5, 6, 7 ------- COMMENT: 2467c1608e001a46 52 ERvJnMOJ0oXZ1FVbeSp/q9F9hPU Fig 5, 6, 7 ------- COMMENT: 2467c1608e001a46 53 +T2RCOnGZ8iCmTcN+gt4KmFBkGI Fig8 Presence of MBF is correlated with cdc10 dependent transcription repression during G2 ------- COMMENT: 2467c1608e001a46 54 +T2RCOnGZ8iCmTcN+gt4KmFBkGI Fig8 Presence of MBF is correlated with cdc10 dependent transcription repression during G2 ------- COMMENT: 2467c1608e001a46 57 +T2RCOnGZ8iCmTcN+gt4KmFBkGI Fig8 Presence of MBF is correlated with cdc10 dependent transcription repression during G2 ------- COMMENT: 2467c1608e001a46 58 +T2RCOnGZ8iCmTcN+gt4KmFBkGI Fig8 Presence of MBF is correlated with cdc10 dependent transcription repression during G2 ------- COMMENT: 2467c1608e001a46 61 zkuOtoj+fEDucjztafr3vavAMHU Fig8 C ------- COMMENT: 2467c1608e001a46 64 /cln0/8SWzp8VsaxQzjbRXB7qeM Fig2 B ------- COMMENT: 2467c1608e001a46 70 jynKJuwS6ZFNpMphXki4zOh9uXk Fig3C Abnormal DNA replication with cut DNA replication in absence cig1, cig2 and cdc13 promoter ON some cells have a cut phenotype. (comment: NOT sure the data warrants an annotation) ------- COMMENT: 2467c1608e001a46 71 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 24a3853610543a99 6 B6WTgJi7UJRAGMvDgeA4YHez3DQ (comment: all taf1 introns affected) ------- COMMENT: 24a8d08a6dd2cb25 1 hRXwSR+iI6QydHGjvyY1kCzmUBM increased number of lipid droples/cell fig 3b/c ------- COMMENT: 24a8d08a6dd2cb25 2 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 24a8d08a6dd2cb25 3 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 24a8d08a6dd2cb25 4 RQVovA0KL8x3nlRwERVcZJ1YEHU fig 4 ------- COMMENT: 24a8d08a6dd2cb25 5 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 24a8d08a6dd2cb25 6 2tGVEBqbCON6NMCQfM25/u05O5o fig 5 (comment: cerulenin) ------- COMMENT: 24b35cab14100485 1 X35OCmdW39wqqhIFY6gzsZhnvGk Over expression does not rescue cdc2-M63 or cdc2-M35 temperature sensitive phenotypes ------- COMMENT: 24b35cab14100485 4 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 24b35cab14100485 7 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 24b35cab14100485 8 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 24b35cab14100485 9 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 24b35cab14100485 10 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 24b35cab14100485 13 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 24b35cab14100485 14 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 24b35cab14100485 15 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 24b35cab14100485 16 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 24b35cab14100485 17 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 24b35cab14100485 31 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 24cc84e2bcc157d2 9 aUIUvJ5XT4DhSyPDWd//S1yR4is ------- COMMENT: 24d8dd57a8133df4 14 5TvHsyylPZs32BSmESz1zVgAT1w We observed similar intensities of interaction of the Nrl1(N-term) and the Nrl1(N-term + NRDE-2) constructs with Mtl1 (1511.2  89.4 or 1558.2  159.3 Miller units for the N-terminal region and the N-terminus with NRDE2 domain and Mtl1, respectively), ------- COMMENT: 24d8dd57a8133df4 15 5TvHsyylPZs32BSmESz1zVgAT1w We observed similar intensities of interaction of the Nrl1(N-term) and the Nrl1(N-term + NRDE-2) constructs with Mtl1 (1511.2  89.4 or 1558.2  159.3 Miller units for the N-terminal region and the N-terminus with NRDE2 domain and Mtl1, respectively), ------- COMMENT: 24d8dd57a8133df4 16 pv6gg0PElu7zYYQg4vQhDFF0d/Y The interactions of the Nrl1(NRDE-2) and the Nrl1(C-term) domain constructs with Mtl1 were significantly lower ------- COMMENT: 24d8dd57a8133df4 19 8AA7TJy78XTjRPdMLxsojkom9ic ------- COMMENT: 24d8dd57a8133df4 23 XuN5yWJt6gP4ojwBd6M7ktx+Ass (comment: Need to curate ref42 for earlier part of this story, but this can be. inferred here from the interactions) ------- COMMENT: 24e9f1d9ea02f952 27 UqdvFXYEzn+stjowMjjnknS0tEw (comment: matmi and matpi) ------- COMMENT: 24e9f1d9ea02f952 29 UqdvFXYEzn+stjowMjjnknS0tEw (comment: matmi and matpi) ------- COMMENT: 24f09f2166d1c063 1 ZU128pU6uzcdZwbX2N8jI0N7pjU asf1-1 alleviated silencing of the ura4+ inserted at the outer centromeric repeat region (otr1R::ura4+) and within a centromere-homologous (cenH) element at the silent mat locus (Kint2::ura4+), in a manner similar to HIRA null mutants (Figure 1C). ------- COMMENT: 24f09f2166d1c063 2 hh7KCBc+XWFgiPkbbu8XMcBpskg asf1-1 and HIRA mutants were defective in mating-type switching, as indicated by the light iodine staining of the colonies (Figure 1F) ------- COMMENT: 24f09f2166d1c063 3 KYcfdqVNsNe1SThWro3cYB7lsZc Defective mating-type switching in heterochromatin mutants results in poor iodine staining of colonies, in contrast to the dark staining of wild-type colonies (Jia et al., 2004). asf1-1 and HIRA mutants were defective in mating-type switching, as indicated by the light iodine staining of the colonies (Figure 1F). ------- COMMENT: 24f09f2166d1c063 4 KYcfdqVNsNe1SThWro3cYB7lsZc Defective mating-type switching in heterochromatin mutants results in poor iodine staining of colonies, in contrast to the dark staining of wild-type colonies (Jia et al., 2004). asf1-1 and HIRA mutants were defective in mating-type switching, as indicated by the light iodine staining of the colonies (Figure 1F). ------- COMMENT: 24f09f2166d1c063 5 KYcfdqVNsNe1SThWro3cYB7lsZc Defective mating-type switching in heterochromatin mutants results in poor iodine staining of colonies, in contrast to the dark staining of wild-type colonies (Jia et al., 2004). asf1-1 and HIRA mutants were defective in mating-type switching, as indicated by the light iodine staining of the colonies (Figure 1F). ------- COMMENT: 24f09f2166d1c063 6 KYcfdqVNsNe1SThWro3cYB7lsZc Defective mating-type switching in heterochromatin mutants results in poor iodine staining of colonies, in contrast to the dark staining of wild-type colonies (Jia et al., 2004). asf1-1 and HIRA mutants were defective in mating-type switching, as indicated by the light iodine staining of the colonies (Figure 1F). ------- COMMENT: 24f09f2166d1c063 7 KYcfdqVNsNe1SThWro3cYB7lsZc Defective mating-type switching in heterochromatin mutants results in poor iodine staining of colonies, in contrast to the dark staining of wild-type colonies (Jia et al., 2004). asf1-1 and HIRA mutants were defective in mating-type switching, as indicated by the light iodine staining of the colonies (Figure 1F). ------- COMMENT: 24f09f2166d1c063 8 KYcfdqVNsNe1SThWro3cYB7lsZc Defective mating-type switching in heterochromatin mutants results in poor iodine staining of colonies, in contrast to the dark staining of wild-type colonies (Jia et al., 2004). asf1-1 and HIRA mutants were defective in mating-type switching, as indicated by the light iodine staining of the colonies (Figure 1F). ------- COMMENT: 24f09f2166d1c063 9 aae3bIvAi2fb6QPlp5O0DB2dpQY Whereas asf1-1 cells showed slight reduction in levels of H3K9me, Swi6 and Chp2, the levels of these factors in hip1Δ appeared comparable to wild type (Figure 2A and 2B). ------- COMMENT: 24f09f2166d1c063 10 aae3bIvAi2fb6QPlp5O0DB2dpQY Whereas asf1-1 cells showed slight reduction in levels of H3K9me, Swi6 and Chp2, the levels of these factors in hip1Δ appeared comparable to wild type (Figure 2A and 2B). ------- COMMENT: 24f09f2166d1c063 11 oSrNQ5QupZDylSCJZ/C1THeG/n4 ChIP-chip showed that Hip1 was enriched throughout heterochromatin domains in the wild-type cells (Figure 2D, 2E, and S3). In the absence of Swi6, Hip1 localization was restricted to transcribed dg/dh repeats, and it failed to spread outward to the surrounding sequences (Figure 2D, 2E, and S3). ------- COMMENT: 24f09f2166d1c063 12 DDaMWnZpOylUApVUvJGP9/o0AcM (comment: CHECK not sure if this is quite the correct term, but it is the old spreading term ------- COMMENT: 24f09f2166d1c063 13 d0CNeQchOvfzu85DseoXchOcEoo Consistent with both TGS and cis-PTGS contributing to heterochromatin silencing, combining asf1-1 or hip1Δ with tas3Δ resulted in synergistic defects in heterochromatic silencing (Figure 3A). ------- COMMENT: 24f09f2166d1c063 14 d0CNeQchOvfzu85DseoXchOcEoo Consistent with both TGS and cis-PTGS contributing to heterochromatin silencing, combining asf1-1 or hip1Δ with tas3Δ resulted in synergistic defects in heterochromatic silencing (Figure 3A). ------- COMMENT: 24f09f2166d1c063 15 BpKK4eKx5yotessTNZijuz62+aM We also found that double mutants carrying mutations in clr3 or mit1 along with either asf1-1 or hip1Δ showed cumulative derepression of repeat elements (Figure 3A and S4), indicating overlapping functions for Asf1/HIRA and SHREC. ------- COMMENT: 24f09f2166d1c063 16 d0CNeQchOvfzu85DseoXchOcEoo Consistent with both TGS and cis-PTGS contributing to heterochromatin silencing, combining asf1-1 or hip1Δ with tas3Δ resulted in synergistic defects in heterochromatic silencing (Figure 3A). ------- COMMENT: 24f09f2166d1c063 17 d0CNeQchOvfzu85DseoXchOcEoo Consistent with both TGS and cis-PTGS contributing to heterochromatin silencing, combining asf1-1 or hip1Δ with tas3Δ resulted in synergistic defects in heterochromatic silencing (Figure 3A). ------- COMMENT: 24f09f2166d1c063 18 BpKK4eKx5yotessTNZijuz62+aM We also found that double mutants carrying mutations in clr3 or mit1 along with either asf1-1 or hip1Δ showed cumulative derepression of repeat elements (Figure 3A and S4), indicating overlapping functions for Asf1/HIRA and SHREC. ------- COMMENT: 24f09f2166d1c063 19 BpKK4eKx5yotessTNZijuz62+aM We also found that double mutants carrying mutations in clr3 or mit1 along with either asf1-1 or hip1Δ showed cumulative derepression of repeat elements (Figure 3A and S4), indicating overlapping functions for Asf1/HIRA and SHREC. ------- COMMENT: 24f09f2166d1c063 20 BpKK4eKx5yotessTNZijuz62+aM We also found that double mutants carrying mutations in clr3 or mit1 along with either asf1-1 or hip1Δ showed cumulative derepression of repeat elements (Figure 3A and S4), indicating overlapping functions for Asf1/HIRA and SHREC. ------- COMMENT: 24f09f2166d1c063 21 HqBr4Tt9blWKXLeBUXyOe3GshyA However, when asf1-1 or hip1Δ were combined with clr6 or alp13 mutant alleles, double mutants did not show additive defects on silencing as compared to the single mutants (Figure 3A and S4). ------- COMMENT: 24f09f2166d1c063 22 HqBr4Tt9blWKXLeBUXyOe3GshyA However, when asf1-1 or hip1Δ were combined with clr6 or alp13 mutant alleles, double mutants did not show additive defects on silencing as compared to the single mutants (Figure 3A and S4). ------- COMMENT: 24f09f2166d1c063 23 OOULeHopSlsMJe8f15ny0qY5D/M Based on the genetic analyses, it was possible that Asf1/HIRA facilitate histone deacetylation by Clr6. Asf1 co-immunoprecipitated with Clr6 complex subunits Alp13 and Clr6 (Figure 3B). Moreover, asf1-1 and hip1Δ exhibited a substantial increase in bulk H3K9ac levels, in a manner similar to alp13Δ (Figure 3C).). To confirm this further, we performed ChIP-chip analyses of H3K9ac. Both alp13Δ and asf1-1 mutants showed widespread increase in H3K9ac, as compared to the wild-type cells. Notably, although 30% of the probes in our microarray correspond to intergenic regions, nearly all probes affected by asf1-1 and alp13Δ reside in coding regions (Figure 3D and 3E). ------- COMMENT: 24f09f2166d1c063 24 OOULeHopSlsMJe8f15ny0qY5D/M Based on the genetic analyses, it was possible that Asf1/HIRA facilitate histone deacetylation by Clr6. Asf1 co-immunoprecipitated with Clr6 complex subunits Alp13 and Clr6 (Figure 3B). Moreover, asf1-1 and hip1Δ exhibited a substantial increase in bulk H3K9ac levels, in a manner similar to alp13Δ (Figure 3C).). To confirm this further, we performed ChIP-chip analyses of H3K9ac. Both alp13Δ and asf1-1 mutants showed widespread increase in H3K9ac, as compared to the wild-type cells. Notably, although 30% of the probes in our microarray correspond to intergenic regions, nearly all probes affected by asf1-1 and alp13Δ reside in coding regions (Figure 3D and 3E). ------- COMMENT: 24f09f2166d1c063 25 OOULeHopSlsMJe8f15ny0qY5D/M Based on the genetic analyses, it was possible that Asf1/HIRA facilitate histone deacetylation by Clr6. Asf1 co-immunoprecipitated with Clr6 complex subunits Alp13 and Clr6 (Figure 3B). Moreover, asf1-1 and hip1Δ exhibited a substantial increase in bulk H3K9ac levels, in a manner similar to alp13Δ (Figure 3C).). To confirm this further, we performed ChIP-chip analyses of H3K9ac. Both alp13Δ and asf1-1 mutants showed widespread increase in H3K9ac, as compared to the wild-type cells. Notably, although 30% of the probes in our microarray correspond to intergenic regions, nearly all probes affected by asf1-1 and alp13Δ reside in coding regions (Figure 3D and 3E). ------- COMMENT: 24f09f2166d1c063 26 OOULeHopSlsMJe8f15ny0qY5D/M Based on the genetic analyses, it was possible that Asf1/HIRA facilitate histone deacetylation by Clr6. Asf1 co-immunoprecipitated with Clr6 complex subunits Alp13 and Clr6 (Figure 3B). Moreover, asf1-1 and hip1Δ exhibited a substantial increase in bulk H3K9ac levels, in a manner similar to alp13Δ (Figure 3C).). To confirm this further, we performed ChIP-chip analyses of H3K9ac. Both alp13Δ and asf1-1 mutants showed widespread increase in H3K9ac, as compared to the wild-type cells. Notably, although 30% of the probes in our microarray correspond to intergenic regions, nearly all probes affected by asf1-1 and alp13Δ reside in coding regions (Figure 3D and 3E). ------- COMMENT: 24f09f2166d1c063 27 j57WCad7jnUeN53161W6Qp4Dfsk Moreover, asf1-1 and hip1Δ showed upregulation of sense and antisense transcripts corresponding to subtelomeric genes located within heterochromatic domains (Figure S5A). ------- COMMENT: 24f09f2166d1c063 28 j57WCad7jnUeN53161W6Qp4Dfsk Moreover, asf1-1 and hip1Δ showed upregulation of sense and antisense transcripts corresponding to subtelomeric genes located within heterochromatic domains (Figure S5A). ------- COMMENT: 24f09f2166d1c063 29 rh/kEZsnu1embH8I44SYOkASfkc Interestingly, asf1-1, but not hip1Δ, also showed substantial increase in the levels of transcripts derived from intergenic portions of rDNA repeat loci (Figure S5B). ------- COMMENT: 24f09f2166d1c063 30 IevXzGPXeZc1mPsAyYWDbmPGRZY Notably, asf1-1 produced a disproportionate increase in antisense transcripts – constituting a large proportion of probes upregulated. Detailed expression profiling of individual loci showed that the antisense transcripts upregulated in asf1-1 mutants were also upregulated in hip1Δ and alp13Δ cells (Figure 4A and 4B). ------- COMMENT: 24f09f2166d1c063 31 XJb7vsS4pWDs1Y8WT4suH9pUGNs Thus, in addition to silencing heterochromatic repeats, Asf1 prevents antisense transcription at euchromatic loci. ------- COMMENT: 24f09f2166d1c063 32 uhFN3cptCSWJ4/OwfVYJd079xvk more sensitive to bleomycin-induced damage, as indicated by the disappearance of full- length chromosome bands and the appearance of a smear of broken DNA fragments (Figure 4E). ------- COMMENT: 24f09f2166d1c063 33 0usAnphU8WRXd37xXTHY69Q8NVY We also found that asf1-1 cells were hypersensitive to genotoxic agents such as bleomycin, camptothecin and methylmethane sulfonate (Figure S6) ------- COMMENT: 24f09f2166d1c063 34 0usAnphU8WRXd37xXTHY69Q8NVY We also found that asf1-1 cells were hypersensitive to genotoxic agents such as bleomycin, camptothecin and methylmethane sulfonate (Figure S6) ------- COMMENT: 24f09f2166d1c063 35 P9lUN4srVZHEX99ywpKwcK+/gNc However, both Asf1 and Clr6 complex-II mutants were not sensitive to hydoxyurea (Figure S6) ------- COMMENT: 24f09f2166d1c063 36 P9lUN4srVZHEX99ywpKwcK+/gNc However, both Asf1 and Clr6 complex-II mutants were not sensitive to hydoxyurea (Figure S6) ------- COMMENT: 24f09f2166d1c063 37 qEdrhew9lFLr7akkkSkffNCvqpg We observed nucleosome free regions at 5′ ends of genes followed by positioned nucleosomes in open reading frames (Figure S7A). Comparison of nucleosome occupancy across heterochromatin domains in asf1, clr3 or asf1clr3 mutant cells to wild-type cells identified several sites showing depletion of nucleosomes in mutant cells. ------- COMMENT: 24f09f2166d1c063 38 +Y7Lsbd9StbXdj9ElZwiCAS9R9M In general, changes observed in clr3 and asf1 single mutants were weaker as compared to asf1clr3 double mutant that showed substantial reduction in the nucleosome occupancy (Figure 5). ------- COMMENT: 24f09f2166d1c063 39 qEdrhew9lFLr7akkkSkffNCvqpg We observed nucleosome free regions at 5′ ends of genes followed by positioned nucleosomes in open reading frames (Figure S7A). Comparison of nucleosome occupancy across heterochromatin domains in asf1, clr3 or asf1clr3 mutant cells to wild-type cells identified several sites showing depletion of nucleosomes in mutant cells. ------- COMMENT: 24f09f2166d1c063 40 qEdrhew9lFLr7akkkSkffNCvqpg We observed nucleosome free regions at 5′ ends of genes followed by positioned nucleosomes in open reading frames (Figure S7A). Comparison of nucleosome occupancy across heterochromatin domains in asf1, clr3 or asf1clr3 mutant cells to wild-type cells identified several sites showing depletion of nucleosomes in mutant cells. ------- COMMENT: 2571bb5c8f7d465c 1 vmj/3upndkP3IaAXzMS4uVqEM8I Figure 12 ------- COMMENT: 2571bb5c8f7d465c 2 vmj/3upndkP3IaAXzMS4uVqEM8I Figure 12 ------- COMMENT: 2571bb5c8f7d465c 4 vmj/3upndkP3IaAXzMS4uVqEM8I Figure 12 ------- COMMENT: 2571bb5c8f7d465c 5 vmj/3upndkP3IaAXzMS4uVqEM8I Figure 12 ------- COMMENT: 2571bb5c8f7d465c 6 vmj/3upndkP3IaAXzMS4uVqEM8I Figure 12 ------- COMMENT: 2571bb5c8f7d465c 7 T1KsGORILkr6fkzrqZCEMF6hRQw (comment: Cobalt/nickel-dependent inorganic pyrophosphatase activity) Figure 3 ------- COMMENT: 2571bb5c8f7d465c 8 etB9PJU1PXok6N0yjWX1c0Ijxqc (comment: Cobalt/nickel-dependent inorganic pyrophosphatase activity) Figure 4 ------- COMMENT: 2571bb5c8f7d465c 9 mIYWLgh7jyIqZ1JrLHVKWncYR+0 (comment: Cobalt/nickel-dependent inorganic pyrophosphatase activity) Figure 1 ------- COMMENT: 2571bb5c8f7d465c 10 etB9PJU1PXok6N0yjWX1c0Ijxqc (comment: Cobalt/nickel-dependent inorganic pyrophosphatase activity) Figure 4 ------- COMMENT: 2571bb5c8f7d465c 11 dGwkFcvP5z056LX+69udD3HbzZc Figures 1 and 3 ------- COMMENT: 2571bb5c8f7d465c 12 dGwkFcvP5z056LX+69udD3HbzZc Figures 1 and 3 ------- COMMENT: 2571bb5c8f7d465c 13 VWRY+xIXdxFg+BJJ5YcgBRZgJC4 Figure 10 ------- COMMENT: 2571bb5c8f7d465c 17 VWRY+xIXdxFg+BJJ5YcgBRZgJC4 Figure 10 ------- COMMENT: 2571bb5c8f7d465c 18 riH6PuZeW9pd2w953irT9kPPwpE Figure 8 ------- COMMENT: 2571bb5c8f7d465c 19 riH6PuZeW9pd2w953irT9kPPwpE Figure 8 ------- COMMENT: 2571bb5c8f7d465c 20 riH6PuZeW9pd2w953irT9kPPwpE Figure 8 ------- COMMENT: 2571bb5c8f7d465c 21 riH6PuZeW9pd2w953irT9kPPwpE Figure 8 ------- COMMENT: 2571bb5c8f7d465c 22 riH6PuZeW9pd2w953irT9kPPwpE Figure 8 ------- COMMENT: 258f4a60a582c915 2 sgMmi3wK+dJBlkGL3QsG5hsV9c0 Fig. 1a ------- COMMENT: 258f4a60a582c915 4 2twvGP9y8IiAqeHRQjUQeDj9vxQ Fig. 1b ------- COMMENT: 258f4a60a582c915 5 luxJO6F18+IOU59jie0SEuR5YCQ Figure 1c & e ------- COMMENT: 258f4a60a582c915 11 55bjc5MxQebqHnL2GwazGRd9bjg rescues increase in sub-telomeric gene expression, lack of MBF-dependent gene induction, growth defects at extreme temperatures, or in the presence of hydroxyurea or camptothecin or low glucose ------- COMMENT: 258f4a60a582c915 12 VZwQ+GtsqYZIXDrqdGMmyfqYK/g rescues increase in sub-telomeric gene expression, lack of MBF-dependent gene inductions, growth defects at extreme temperatures, or in the presence of hydroxyurea or camptothecin or low glucose ------- COMMENT: 258f4a60a582c915 13 UgsrplRWrIQvpIcoof0+bJE4GSc rescues increase in sub-telomeric gene expression, lack of MBF-dependent gene inductions, growth in the presence of hydroxyurea ------- COMMENT: 258f4a60a582c915 14 Ns/m8JB8El7Gz3HIyabK/wjVOYY rescues increase in sub-telomeric gene expression ------- COMMENT: 258f4a60a582c915 15 Ns/m8JB8El7Gz3HIyabK/wjVOYY rescues increase in sub-telomeric gene expression ------- COMMENT: 258f4a60a582c915 16 Ns/m8JB8El7Gz3HIyabK/wjVOYY rescues increase in sub-telomeric gene expression ------- COMMENT: 258f4a60a582c915 17 /QK4lBErIHEJ0K1G2jAqrwAVjGI Tor1 inhibits the binding of Gcn5 at sub-telomeric genes and MBF promoters ------- COMMENT: 258f4a60a582c915 20 2crYr+eAIZ5eHDjBhKfZI7L7az4 S2D Fig ------- COMMENT: 258f4a60a582c915 21 2crYr+eAIZ5eHDjBhKfZI7L7az4 S2D Fig ------- COMMENT: 258f4a60a582c915 22 ZYqW1qMYjXVcuWdM6NjgZC0QB0Y 30 fold. Fig 2 ------- COMMENT: 258f4a60a582c915 23 JdOtMl2cpIkbXzpBZKQZ+lAqK/8 280 fold. Fig 2 ------- COMMENT: 258f4a60a582c915 24 MBb7sSKnum6NdyMbFBxGzTioSk4 120 fold. Fig 2 ------- COMMENT: 258f4a60a582c915 25 6EDmXebijBUa0tFXmdYXeutbaGk Figure2 A ------- COMMENT: 258f4a60a582c915 26 6EDmXebijBUa0tFXmdYXeutbaGk Figure2 A ------- COMMENT: 258f4a60a582c915 27 6EDmXebijBUa0tFXmdYXeutbaGk Figure2 A ------- COMMENT: 258f4a60a582c915 28 6EDmXebijBUa0tFXmdYXeutbaGk Figure2 A ------- COMMENT: 258f4a60a582c915 29 6EDmXebijBUa0tFXmdYXeutbaGk Figure2 A ------- COMMENT: 258f4a60a582c915 30 6EDmXebijBUa0tFXmdYXeutbaGk Figure2 A ------- COMMENT: 258f4a60a582c915 31 6EDmXebijBUa0tFXmdYXeutbaGk Figure2 A ------- COMMENT: 258f4a60a582c915 32 6EDmXebijBUa0tFXmdYXeutbaGk Figure2 A ------- COMMENT: 258f4a60a582c915 33 6EDmXebijBUa0tFXmdYXeutbaGk Figure2 A ------- COMMENT: 258f4a60a582c915 37 ITkyzbWy4LFOANivFp/M43VBbg0 We detected a markedly higher level of Gcn5 binding at subte- lomeric genes in Δtor1 cells, compared with wild type cells (Fig 4A). ------- COMMENT: 258f4a60a582c915 38 s9hhi2SzhYBTfbmQq5wHIGik0uo Consistently, we detected higher levels of H3K9Ac at subtelomeric genes in Δtor1 cells compared to wild type cells, and this defect was suppressed by either Δgcn5 or Δbdf2 (Fig 4C) ------- COMMENT: 258f4a60a582c915 39 s9hhi2SzhYBTfbmQq5wHIGik0uo Consistently, we detected higher levels of H3K9Ac at subtelomeric genes in Δtor1 cells compared to wild type cells, and this defect was suppressed by either Δgcn5 or Δbdf2 (Fig 4C) ------- COMMENT: 258f4a60a582c915 40 s9hhi2SzhYBTfbmQq5wHIGik0uo Consistently, we detected higher levels of H3K9Ac at subtelomeric genes in Δtor1 cells compared to wild type cells, and this defect was suppressed by either Δgcn5 or Δbdf2 (Fig 4C) ------- COMMENT: 258f4a60a582c915 41 uzHrEl3SZkjdPafdqQ4HfelyTlc The Δbdf2 muta- tion also suppresses the elevated levels of Gcn5 at the subtelomeric chromatin in Δtor1 cells (Fig 4E). ------- COMMENT: 258f4a60a582c915 43 9B0e45i/CA9V0EQ5Ap4xl/1rQAE Unexpectedly, although there is no increase in MBF-dependent transcription, we detected an increase in Gcn5 binding at the promoters of cdc22+ or cdc18+ in Δtor1 or Δgad8 cells under normal or replication stress conditions (Fig 5A) ------- COMMENT: 258f4a60a582c915 45 9B0e45i/CA9V0EQ5Ap4xl/1rQAE Unexpectedly, although there is no increase in MBF-dependent transcription, we detected an increase in Gcn5 binding at the promoters of cdc22+ or cdc18+ in Δtor1 or Δgad8 cells under normal or replication stress conditions (Fig 5A) ------- COMMENT: 258f4a60a582c915 48 9B0e45i/CA9V0EQ5Ap4xl/1rQAE Unexpectedly, although there is no increase in MBF-dependent transcription, we detected an increase in Gcn5 binding at the promoters of cdc22+ or cdc18+ in Δtor1 or Δgad8 cells under normal or replication stress conditions (Fig 5A) ------- COMMENT: 258f4a60a582c915 50 SYjBQF1bf168sMM+kv/rWBXb4XI (comment: no supression) ------- COMMENT: 258f4a60a582c915 53 zLCaBP/CZeSiEUPpMpdy/6uCbfE Significantly, Δbdf2 restores low levels of Gcn5 binding at MBF promoters in Δtor1 cells under normal growth conditions and also restores the normal pattern of an increased level of Gcn5 in response to HU (Fig 6C). ------- COMMENT: 258f4a60a582c915 54 Iu0F21J+NeDM9eIxVoeMlqch0oY (comment: 24%) ------- COMMENT: 258f4a60a582c915 55 XhFk42+F7J5SyY4h37VUbpqsXKs (comment: 25%) ------- COMMENT: 259aebc0ce336450 1 TdI9+f5nKDENfchGFxxKCcyaRDg figure 8a ------- COMMENT: 259aebc0ce336450 2 TdI9+f5nKDENfchGFxxKCcyaRDg figure 8a ------- COMMENT: 259aebc0ce336450 3 etgd+VbWOE5PsIOn8OTS/2+2BzI figure 8 b ------- COMMENT: 259aebc0ce336450 4 TdI9+f5nKDENfchGFxxKCcyaRDg figure 8a ------- COMMENT: 259aebc0ce336450 5 G1oB/YUEyuKFEj5o1LWs8OVi1Y0 figure 8c ------- COMMENT: 25a5d9e72c9fc7ec 1 lkank+89A36CHingEQ4OsGbG3MA Figure 1a (comment: they don't really show that the modification is phosphorylation, but considering the rest of the data this annotation seems ok) ------- COMMENT: 25a5d9e72c9fc7ec 2 LZGiCiD8rrCuYLUYm4EL34SBsdc Figure 1b ------- COMMENT: 25a5d9e72c9fc7ec 3 w4RTJf8pFE9NsHD+bC+zYd4PZ8o (comment: they don't show the "added during" data so this is a bit anecdotal from the text) ------- COMMENT: 25a5d9e72c9fc7ec 4 w4RTJf8pFE9NsHD+bC+zYd4PZ8o (comment: they don't show the "added during" data so this is a bit anecdotal from the text) ------- COMMENT: 25a5d9e72c9fc7ec 5 f6hXO/gzIB08OGKQVkGln9R+gDI in text relevant to fig1) ------- COMMENT: 25a5d9e72c9fc7ec 6 f6hXO/gzIB08OGKQVkGln9R+gDI in text relevant to fig1 ------- COMMENT: 25a5d9e72c9fc7ec 7 LZGiCiD8rrCuYLUYm4EL34SBsdc Figure 1b ------- COMMENT: 25a5d9e72c9fc7ec 8 EaJ1cIJCgxH1h7dmT6mEA3YTf4A Figure 1d ------- COMMENT: 25a5d9e72c9fc7ec 9 EaJ1cIJCgxH1h7dmT6mEA3YTf4A Figure 1d ------- COMMENT: 25a5d9e72c9fc7ec 10 EaJ1cIJCgxH1h7dmT6mEA3YTf4A Figure 1d ------- COMMENT: 25a5d9e72c9fc7ec 11 XmVslno7t/UpNWUl10NgHjS5eQg Figure 2a (comment: 20 mins after synchronized released into mitosis. I wouldn't want to guess exactly what stage of mitosis this is) ------- COMMENT: 25a5d9e72c9fc7ec 12 N6gdfNLkuPfyqKNbJqqQxYyAr9g Figure S3 ------- COMMENT: 25a5d9e72c9fc7ec 13 N6gdfNLkuPfyqKNbJqqQxYyAr9g Figure S3 ------- COMMENT: 25a5d9e72c9fc7ec 14 N6gdfNLkuPfyqKNbJqqQxYyAr9g Figure S3 ------- COMMENT: 25a5d9e72c9fc7ec 15 N6gdfNLkuPfyqKNbJqqQxYyAr9g Figure S3 ------- COMMENT: 25a5d9e72c9fc7ec 16 BO/plU47gDdcXvied1ncR9Hl1GQ Figure 2bc,5 ------- COMMENT: 25a5d9e72c9fc7ec 17 pJvU2xJED7s1s/vILNkbOq2yOeY Figure 2bc ------- COMMENT: 25a5d9e72c9fc7ec 18 coBTmtFSwuxkBMiY8P2UDllndpE Figure 2b ------- COMMENT: 25a5d9e72c9fc7ec 19 coBTmtFSwuxkBMiY8P2UDllndpE Figure 2b ------- COMMENT: 25a5d9e72c9fc7ec 20 iLbPP7mZ4NCcH1/6bXlQ9CebB64 text to fig2 ------- COMMENT: 25a5d9e72c9fc7ec 21 iLbPP7mZ4NCcH1/6bXlQ9CebB64 text to fig2 ------- COMMENT: 25a5d9e72c9fc7ec 22 IQNBnecHrt7nHXIVo1OXc4Riqzk (comment: it looks like it is involved in MAINTAINING the checkpoint) fig S4A and 2C ------- COMMENT: 25a5d9e72c9fc7ec 23 i5SNJkwbMuQxheiYYEgH+v7/jmc Fig S4A and 2C ------- COMMENT: 25a5d9e72c9fc7ec 24 i5SNJkwbMuQxheiYYEgH+v7/jmc Fig S4A and 2C ------- COMMENT: 25a5d9e72c9fc7ec 25 BO/plU47gDdcXvied1ncR9Hl1GQ Figure 2bc,5 ------- COMMENT: 25a5d9e72c9fc7ec 27 pJvU2xJED7s1s/vILNkbOq2yOeY Figure 2bc ------- COMMENT: 25a5d9e72c9fc7ec 28 BO/plU47gDdcXvied1ncR9Hl1GQ Figure 2bc,5 ------- COMMENT: 25a5d9e72c9fc7ec 40 glJW1aOyj4jhjS/C9sk9B+zU/dU Figure 4a ------- COMMENT: 25a5d9e72c9fc7ec 41 glJW1aOyj4jhjS/C9sk9B+zU/dU Figure 4a ------- COMMENT: 25a5d9e72c9fc7ec 42 gax+5vOr1u+OrlZRL638YEnFy8s Figure 5a ------- COMMENT: 25a5d9e72c9fc7ec 43 gax+5vOr1u+OrlZRL638YEnFy8s Figure 5a ------- COMMENT: 25a5d9e72c9fc7ec 44 gax+5vOr1u+OrlZRL638YEnFy8s Figure 5a ------- COMMENT: 25a5d9e72c9fc7ec 45 gax+5vOr1u+OrlZRL638YEnFy8s Figure 5a ------- COMMENT: 25a5d9e72c9fc7ec 46 gax+5vOr1u+OrlZRL638YEnFy8s Figure 5a ------- COMMENT: 25a5d9e72c9fc7ec 47 gax+5vOr1u+OrlZRL638YEnFy8s Figure 5a ------- COMMENT: 25a5d9e72c9fc7ec 48 BO/plU47gDdcXvied1ncR9Hl1GQ Figure 2bc,5 ------- COMMENT: 25a5d9e72c9fc7ec 49 F/v5ZYZtvGcAjqQpg/FT6C7sUG4 Fig6 (comment: they incubate with 3 different E2s so can't specify a substrate) ------- COMMENT: 25a5d9e72c9fc7ec 51 CLApI+JyRA0yHmQoPD9aJ6dAo1A (comment: 3 E2s mixed in the same assay so can't specify a substrate) ------- COMMENT: 25aefd25e9ecb150 8 tN/dyDxxphb/QcUkcoQZMj5eZUA Figure 2a ------- COMMENT: 25aefd25e9ecb150 9 gI7M/cfXotHX4fnp7vEHLfTVSiM Fig 1i ------- COMMENT: 25aefd25e9ecb150 10 gI7M/cfXotHX4fnp7vEHLfTVSiM Fig 1i ------- COMMENT: 25aefd25e9ecb150 11 cIb3Za6y+h8N9WcDRGQnYaHA5XQ Figure 2b ------- COMMENT: 25aefd25e9ecb150 12 gUdTUbPe7G4/XvjDsiuEAEfEri0 Figure 2c ------- COMMENT: 25aefd25e9ecb150 14 5bpDKcZWRBvP7y9/iZjh/rb8NYw (comment: cytoplasm in interphase) (Figure 4a, I) ------- COMMENT: 25aefd25e9ecb150 15 ixRkOLFohv02Tg0l7oDklz6nMyQ Figure 4b ------- COMMENT: 25aefd25e9ecb150 17 RkaoK7/R6qj4SC/l/UFPaMvDWmI fig 5 (comment: CHECK during mitotic M-phase) ------- COMMENT: 25aefd25e9ecb150 18 RkaoK7/R6qj4SC/l/UFPaMvDWmI fig 5 (comment: CHECK during mitotic M-phase) ------- COMMENT: 25aefd25e9ecb150 19 RkaoK7/R6qj4SC/l/UFPaMvDWmI fig 5 (comment: CHECK during mitotic M-phase) ------- COMMENT: 25aefd25e9ecb150 20 RkaoK7/R6qj4SC/l/UFPaMvDWmI fig 5 (comment: CHECK during mitotic M-phase) ------- COMMENT: 25aefd25e9ecb150 21 RkaoK7/R6qj4SC/l/UFPaMvDWmI fig 5 (comment: CHECK during mitotic M-phase) ------- COMMENT: 25aefd25e9ecb150 22 RkaoK7/R6qj4SC/l/UFPaMvDWmI fig 5 (comment: CHECK during mitotic M-phase) ------- COMMENT: 25aefd25e9ecb150 23 EUD8IjtRcLWoc1kwaJMN2nn9YaU (comment: CHECK cytoplasm during interphase with nuclear localization) ------- COMMENT: 25aefd25e9ecb150 24 q3XHYixyZerPQAyF2eLtLyi9Rl0 fig 5 (comment: CHECK during interphase) ------- COMMENT: 25aefd25e9ecb150 25 0p/ZYdRZZpb4AQ/NGVPlRSUBkV8 fig 5 (comment: CHECK during mitosis) ------- COMMENT: 25c4a68249a1036f 1 B4bT3mBnytmBalsbSR/5jKNTJMk (comment: The mutant strain appears to grow normally) (figure 2b). ------- COMMENT: 25c4a68249a1036f 2 BbJ5nDtmoE+l7pZRxmjQX1xr9tM (comment: ...decreased the average population cell length from 12 µm to 10.3 µm in the wild-type strain after incubation for 6 h at 29°C, whereas no such decrease by O1 was observed in the rho1-A62T strain, where average cell size is 12.3 µm in the DMSO control and 11.8 µm in the presence of O1 (figure 2c).) ------- COMMENT: 25e3a75033029bd6 1 5E//KoJdlCPQAP1wRVIS8ykyB4s Figure 1E (comment: unbundled microtubules seen in early mitosis) ------- COMMENT: 25e3a75033029bd6 2 9BL61n6PUta4AwIMgQwcGF+v17Y (comment: Microtubule bundling partially rescued at the restrictive temperature) ------- COMMENT: 25e3a75033029bd6 3 oe12HREPDmuL7zMYO0LvplEP7jc (comment: shown with the peg1-104 allele) ------- COMMENT: 25e3a75033029bd6 4 my1S9t3JPzHctrK+REDnYBCW8B8 fig 3a (comment: by cen2-GFP observation) ------- COMMENT: 25e3a75033029bd6 5 HBL6UR1SGUpWge6L5BoeOY2F8pA Figure S1 ------- COMMENT: 25e3a75033029bd6 6 QVZ0ZAfX/9/Ilj+cMAovAmC+hv4 Supp S2A/4 ------- COMMENT: 25e3a75033029bd6 7 R0ybwCfuPvIoUaX4v9l28IsZc4Y Supp S2 ------- COMMENT: 25e3a75033029bd6 8 BJ79CCdIs9uCpO29r+29Uejo1Lo FIgure 1A ------- COMMENT: 25e3a75033029bd6 9 bJzU56P7qC7fzCuXgYA7Z36m7BY figure S4 ------- COMMENT: 25e3a75033029bd6 10 LkGQQw8FQBPnHuF6zSR3Jquhnkc fig 1C ------- COMMENT: 25e3a75033029bd6 11 LkGQQw8FQBPnHuF6zSR3Jquhnkc fig 1C ------- COMMENT: 25e3a75033029bd6 12 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: 25e3a75033029bd6 15 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: 25e3a75033029bd6 16 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: 25e3a75033029bd6 17 BiMhHRPB1xWYY9F0SpICnetmWQk fig 3I ------- COMMENT: 25e3a75033029bd6 18 dM0hn9FVBon893V9hjBgKaIhZoc S2A ------- COMMENT: 25e3a75033029bd6 19 QVZ0ZAfX/9/Ilj+cMAovAmC+hv4 S2A/4 ------- COMMENT: 25e3a75033029bd6 20 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 25e3a75033029bd6 21 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 25e3a75033029bd6 22 5E//KoJdlCPQAP1wRVIS8ykyB4s Figure 1E (comment: unbundled microtubules seen in early mitosis) ------- COMMENT: 260532ed016fea91 1 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: 260532ed016fea91 2 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: 260532ed016fea91 6 Of4a1FCdIGyAaKqHtbP5SHNSuHY figure 9 ------- COMMENT: 260532ed016fea91 7 rijGoDh/2exRxt4N7bAvsH1xYXs figure 9 (comment: modified form is activated for sexual differentiation) ------- COMMENT: 260925d9ac1a773d 9 rPrN6BZxnKpx/PxMLIkPJzXzlUk (comment: CHECK aa1-55) ------- COMMENT: 260925d9ac1a773d 12 SbbzAZeqkARxudOz9X8S8DhkZ8Q Yeast two hybrid To investigate how phosphorylation of the CIM domain affects the interaction between Ccp1 and CENP-TCnp20, we conducted yeast two-hybrid assays with Cnp20-14D and Ccp1. We found that the interaction between Cnp20-14D and Ccp1 was dramatically reduced (Fig. 5F). ------- COMMENT: 260925d9ac1a773d 14 vI7oacJ6QIO2Z4Rgey03svI9ovk Cnp20-14A showed a strong interaction with Ccp1 (Fig. 5F) ------- COMMENT: 260925d9ac1a773d 15 s0+BdZVEZgYlEt/y080iMtO2/N8 However, we found that Spc25-GFP appeared not to attach to microtubules in ∼20% of cnp20-14A mitotic cells, indicating that dephosphorylation of the CIM domain leads to mislocalization of Ndc80C during mitosis (Fig. 7A). Importantly, our co-IP results indicated that the interaction between Cnp20-14A and Ndc80 is significantly reduced during mitosis (Fig. 7B). ------- COMMENT: 260925d9ac1a773d 16 PaWisCOUYU4T03ghcfzdtO/vDkk (Fig. 4 E and F). ------- COMMENT: 260925d9ac1a773d 20 SZQHnPShUaGbxI36+0MeJUAHPI4 fig 3 Our results indicate that the first 55 amino acids of CENP-TCnp20 are the minimal interaction domain with Ccp1, which we named the Ccp1- interacting motif (CIM). ------- COMMENT: 260925d9ac1a773d 21 iaSuiIFPBqAgAXqBMR0Pw3773BI We found that cnp20-14A is highly sensitive to TBZ, even more strongly than the cnp20-ΔCIM mutant (Fig. 6D and SI Appendix, Fig. S16), ------- COMMENT: 260925d9ac1a773d 23 fRvC3hjaVaANPPW8R1Lda8yqxX0 Yeast two hybrid The yeast two-hybrid assays showed that the Ccp1 homodimer mutant, Ccp1-4A, was unable to interact with CENP-TCnp20 (Fig. 3A and SI Appendix, Fig. S8) ------- COMMENT: 260925d9ac1a773d 24 uzGO1MkWlDj7yp8dWWHd6Z4eTbM fig 3 Our results indicate that the first 55 amino acids of CENP-TCnp20 are the minimal interaction domain with Ccp1, which we named the Ccp1- interacting motif (CIM). Yeast two hybrid ------- COMMENT: 260925d9ac1a773d 27 mBQBEFDONu8M8O8B5I2n112UFoE Consistent with the key role of CENP-TCnp20 in the assembly of the Ndc80 complex, we found that the association of Ndc80-GFP with centromeres is lost in cnp20-9 at the restrictive temperature (Fig. 2 C and D). ------- COMMENT: 260925d9ac1a773d 28 NEEz4IHImck31ToT8SdHaJ5ZqAI We found that GFP-Ccp1 was delocalized from centromeres at the restrictive temperature in cnp20-9 at all stages of the cell cycle (Fig. 2 E and F and SI Appendix, Fig. S3) ------- COMMENT: 260925d9ac1a773d 29 CL3d3T7RQvM7LJ58MT/m6RnjdmU whereas the localization of GFP-Ccp1 at centromeres only has a mild reduction in the CENP-A ts mutant, cnp1-1 (SI Appendix, Fig. S4). ------- COMMENT: 260925d9ac1a773d 30 IPY/6DCHtQdRaHHJYvW1UcwEFQg We next checked the distribution of CENP-TCnp20-GFP in ccp1Δ and found that its centromere localization was unaffected in the mutant (Fig. 2 G–I). ------- COMMENT: 260925d9ac1a773d 31 CL3d3T7RQvM7LJ58MT/m6RnjdmU whereas the localization of GFP-Ccp1 at centromeres only has a mild reduction in the CENP-A ts mutant, cnp1-1 (SI Appendix, Fig. S4). ------- COMMENT: 260925d9ac1a773d 32 yph/oc9sMPZ+7648qJ0t1Y6xsZQ In addition, we found that CENP-ACnp1-GFP partially reduced its centromere localization in cnp20-9 at the restrictive temperature (SI Appendix, Fig. S6). ------- COMMENT: 260925d9ac1a773d 33 hfpmXkVqFHmA+w1fhBpVHIw17Fo (These data suggest that phosphorylation of the CIM domain leads to disassociation of Ccp1 from centromeres. CIM domain) Together,our data indicate that CENP-TCnp20 is required for Ccp1 centromere localization ------- COMMENT: 260925d9ac1a773d 35 fB8Fo5v5psr2lJK3aYzz3jBALaI But GFP-Ccp1 is completely disassociated from centromeric regions in cnp20-ΔCIM at all stages of the cell cycle (Fig. 4 C and D and SI Appendix, Fig. S13). ------- COMMENT: 260925d9ac1a773d 37 EuZVJf0xEBlvb+HBquhljGPvYQ8 (comment: CHECK during mitotc M-phase) In contrast, we found that GFP-Ccp1 in all cnp20-14A mutant cells remains associated with centromeres during all the stages of the cell cycle (Fig. 6 B and C). ------- COMMENT: 260925d9ac1a773d 38 mq7SxA37KpEkWr3ky6nvHHSqu5Y But most cnp20-14A cells display mitotic delay, and more than 12% of mutant cells failed to complete chromosome segregation within 30 min (Fig. 6 E and F) ------- COMMENT: 260925d9ac1a773d 39 cfQClJcmpk+joM9AgUnV6xymV84 ((comment: CHECK ndc80) These co-IP experiments showed that the interaction between Ndc80 and CENP-TCnp20 is drastically increased during mitosis (Fig. 7C), supporting the idea that the presence of Ccp1 interferes with the interaction between Ndc80 and CENP-TCnp2 ------- COMMENT: 260925d9ac1a773d 40 VifiXdGESsOelMR/wQ59XFm78Q8 These data demonstrate that CDK1 is capable of phosphorylating the CIM domain of CENP-TCnp20. Various slow migrating bands were observed in the assay with Cnp201-55 (Fig. 7D), indicating that the domain contains multiple phosphorylation sites, consistent with our point mutation analysis. (ASSAYED USING HUMAN CDK1) ------- COMMENT: 260a55b4758c2119 65 7UH+7poNlrg5t4CIh5eq5hhpnfA inferred from direct physical interactions between tea4,tea1 and tea4,for3, plus tea4delta phenotype ------- COMMENT: 260b0436e133fc19 1 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: 260be70a4dcbc884 1 DjOZ7LE0QiEUFqmAwMkZIjXYxnA Fig. S2A-D ------- COMMENT: 260be70a4dcbc884 2 lSZuVGf5q8CtFe8r4qIx5mq90TM Fig. S2C,D ------- COMMENT: 260be70a4dcbc884 3 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 260be70a4dcbc884 4 EJrKWyBr/NaQhxxvZLUQXKV3oNU Fig. S2E ------- COMMENT: 260be70a4dcbc884 5 3+OJYbcRFcCCWqyO9uR3sBpMDNk Fig. 1B; ------- COMMENT: 260be70a4dcbc884 9 3+OJYbcRFcCCWqyO9uR3sBpMDNk Fig. 1B; ------- COMMENT: 260be70a4dcbc884 10 3+OJYbcRFcCCWqyO9uR3sBpMDNk Fig. 1B; ------- COMMENT: 260be70a4dcbc884 11 3+OJYbcRFcCCWqyO9uR3sBpMDNk Fig. 1B; ------- COMMENT: 260be70a4dcbc884 12 3+OJYbcRFcCCWqyO9uR3sBpMDNk Fig. 1B; ------- COMMENT: 260be70a4dcbc884 13 3+OJYbcRFcCCWqyO9uR3sBpMDNk Fig. 1B; ------- COMMENT: 260be70a4dcbc884 14 3+OJYbcRFcCCWqyO9uR3sBpMDNk Fig. 1B; ------- COMMENT: 260be70a4dcbc884 15 3+OJYbcRFcCCWqyO9uR3sBpMDNk Fig. 1B; ------- COMMENT: 260be70a4dcbc884 16 3+OJYbcRFcCCWqyO9uR3sBpMDNk Fig. 1B; ------- COMMENT: 260be70a4dcbc884 17 3+OJYbcRFcCCWqyO9uR3sBpMDNk Fig. 1B; ------- COMMENT: 260be70a4dcbc884 18 3+OJYbcRFcCCWqyO9uR3sBpMDNk Fig. 1B; ------- COMMENT: 260be70a4dcbc884 19 3+OJYbcRFcCCWqyO9uR3sBpMDNk Fig. 1B; ------- COMMENT: 260be70a4dcbc884 20 3+OJYbcRFcCCWqyO9uR3sBpMDNk Fig. 1B; ------- COMMENT: 260be70a4dcbc884 21 3+OJYbcRFcCCWqyO9uR3sBpMDNk Fig. 1B; ------- COMMENT: 260be70a4dcbc884 22 s57ImuqYnAXZCAmpNpa0IxlbyQU Fig. 2A-C; ------- COMMENT: 260be70a4dcbc884 23 s57ImuqYnAXZCAmpNpa0IxlbyQU Fig. 2A-C; ------- COMMENT: 260be70a4dcbc884 24 HEVNkEtywGKTPf7OSPvRVCB7X74 Fig. 2D; ------- COMMENT: 260be70a4dcbc884 25 CGl/rnxhanOzsM3amqV0k5ZLmxc Fig. 3; ------- COMMENT: 260be70a4dcbc884 26 0+EkPIUimbA3Y0fufMhcDx7W5ic Fig. 4A; ------- COMMENT: 260be70a4dcbc884 27 Muokpau82gFZ2H7oPbo0UrQedqw Fig. 4B; ------- COMMENT: 260be70a4dcbc884 28 +x/++9KUq3NiE1OdMRQNKBaZ2wI Fig. 4C; ------- COMMENT: 260be70a4dcbc884 29 /ZsDFIoOUJg4X+/ZUoHMWbKCz+E Fig. 4D; ------- COMMENT: 260be70a4dcbc884 32 tyPX6gbbmbllnPJhBsO7HpWnjAU Fig. 5B; ------- COMMENT: 260be70a4dcbc884 33 tyPX6gbbmbllnPJhBsO7HpWnjAU Fig. 5B; ------- COMMENT: 260be70a4dcbc884 34 tyPX6gbbmbllnPJhBsO7HpWnjAU Fig. 5B; ------- COMMENT: 260be70a4dcbc884 35 tyPX6gbbmbllnPJhBsO7HpWnjAU Fig. 5B; ------- COMMENT: 260be70a4dcbc884 36 tyPX6gbbmbllnPJhBsO7HpWnjAU Fig. 5B; ------- COMMENT: 260be70a4dcbc884 37 w+n5WKPpAYCl0VzaKN4bBRqe8/U Fig. 5C; ------- COMMENT: 260be70a4dcbc884 38 w+n5WKPpAYCl0VzaKN4bBRqe8/U Fig. 5C; ------- COMMENT: 260be70a4dcbc884 39 w+n5WKPpAYCl0VzaKN4bBRqe8/U Fig. 5C; ------- COMMENT: 260be70a4dcbc884 40 w+n5WKPpAYCl0VzaKN4bBRqe8/U Fig. 5C; ------- COMMENT: 260be70a4dcbc884 44 9ACH+8IGPBT0VJzK12j5g9yhISE (commment: exacerbated off-center septa phenotype when efr3null and its3-1 are combined) ------- COMMENT: 260be70a4dcbc884 45 d3v7TGf1u+Ne7SqIp4qs5mqkb1Y (commment: misplaced septa phenotype of its3-1 suppressed by syj1null) ------- COMMENT: 260be70a4dcbc884 46 ZmAQjeaHmi4s7mkc4N7zudIWgwQ Fig. 5A; ------- COMMENT: 260be70a4dcbc884 47 ZmAQjeaHmi4s7mkc4N7zudIWgwQ Fig. 5A; ------- COMMENT: 260be70a4dcbc884 48 ZmAQjeaHmi4s7mkc4N7zudIWgwQ Fig. 5A; ------- COMMENT: 260be70a4dcbc884 49 ZmAQjeaHmi4s7mkc4N7zudIWgwQ Fig. 5A; ------- COMMENT: 260be70a4dcbc884 50 ZmAQjeaHmi4s7mkc4N7zudIWgwQ Fig. 5A; ------- COMMENT: 260be70a4dcbc884 52 7m0i1cIMOZwBDiOygFBfDJMm/R0 Fig. S1F; ------- COMMENT: 260be70a4dcbc884 53 3+OJYbcRFcCCWqyO9uR3sBpMDNk Fig. 1B; ------- COMMENT: 260be70a4dcbc884 54 7m0i1cIMOZwBDiOygFBfDJMm/R0 Fig. S1F; ------- COMMENT: 260be70a4dcbc884 55 7m0i1cIMOZwBDiOygFBfDJMm/R0 Fig. S1F; ------- COMMENT: 260be70a4dcbc884 56 7m0i1cIMOZwBDiOygFBfDJMm/R0 Fig. S1F; ------- COMMENT: 260be70a4dcbc884 57 ifrqlcy7G0f4EFipSEbm7b0gMNU Fig. S2A ------- COMMENT: 262db1ff3a0a0b79 1 xtbiP+vA8wgprzfq3GoVcIWR8J8 (comment: site A) ------- COMMENT: 262db1ff3a0a0b79 2 skQ89AgdC+RbB3rcxG60c/cJQ04 (comment:site B) ------- COMMENT: 262db1ff3a0a0b79 3 skQ89AgdC+RbB3rcxG60c/cJQ04 (comment: site B) ------- COMMENT: 266a57bb225cd9a6 1 tXxwht8viQLZKSzmjq1Gc1G7vts Figure 1A and C ------- COMMENT: 266a57bb225cd9a6 2 VDBXERSDbd3YHK4rET7ChvUnjOc (comment: vw: assayed by increased mad2 at kinetochore - checkpoint active) ------- COMMENT: 266a57bb225cd9a6 3 VDBXERSDbd3YHK4rET7ChvUnjOc (comment: vw: assayed by increased mad2 at kinetochore - checkpoint active) ------- COMMENT: 266a57bb225cd9a6 4 IMHms2FWtBIsCBN6UmElE7jDX6Q figure 1 B ------- COMMENT: 266a57bb225cd9a6 5 xvGNWo1szMIOrdpATXQV0anR8GE Figure 1B, rows 4±6 ------- COMMENT: 266a57bb225cd9a6 8 tXxwht8viQLZKSzmjq1Gc1G7vts Figure 1A and C ------- COMMENT: 266a57bb225cd9a6 25 CPI0+8b+g8XfTqXvMcHXuqKubbI (Figure 1B, rows 4±6 ) ------- COMMENT: 266a57bb225cd9a6 26 CPI0+8b+g8XfTqXvMcHXuqKubbI (Figure 1B, rows 4±6) ------- COMMENT: 2677a08d5a4fb5c7 9 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 2677a08d5a4fb5c7 10 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 2677a08d5a4fb5c7 11 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 2677a08d5a4fb5c7 12 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 2677a08d5a4fb5c7 13 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: 2677a08d5a4fb5c7 14 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: 2677a08d5a4fb5c7 15 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: 2677a08d5a4fb5c7 16 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: 2677a08d5a4fb5c7 17 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: 2677a08d5a4fb5c7 18 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: 2677a08d5a4fb5c7 19 u4h9s5wkcnm3kQoaW9fY2oQ7YqM fig 7A ------- COMMENT: 2677a08d5a4fb5c7 20 DZS7W3n1AxDLDoylhWgz+zM+t0Y fig 7A (comment: depends on actin) ------- COMMENT: 2677a08d5a4fb5c7 22 y8dGkGAAaCRfnankFqO/ZBtQLko fig8 (comment: myo2 clumped in nodes instead of ring) ------- COMMENT: 2677a08d5a4fb5c7 23 y8dGkGAAaCRfnankFqO/ZBtQLko fig8 (comment: myo2 clumped in nodes instead of ring) ------- COMMENT: 2677a08d5a4fb5c7 24 uP2b450kx3y/u6z3D7bDUa+eEuM fig8 ------- COMMENT: 2677a08d5a4fb5c7 25 uP2b450kx3y/u6z3D7bDUa+eEuM fig8 ------- COMMENT: 2677a08d5a4fb5c7 26 uP2b450kx3y/u6z3D7bDUa+eEuM fig8 ------- COMMENT: 2677a08d5a4fb5c7 27 uP2b450kx3y/u6z3D7bDUa+eEuM fig8 ------- COMMENT: 2677a08d5a4fb5c7 28 ZfN0m2l87EUkxznt7S5UgmqZYSc fig9 (comment: mainrtenance) ------- COMMENT: 2677a08d5a4fb5c7 29 JvqKiF7syn97ZfVC9qlsi0ZVvfw fig9 ------- COMMENT: 269782b0ebb602b9 2 UCtIUGrT9m9gm1WmjxyA3wHnAoI figure 4a ------- COMMENT: 269782b0ebb602b9 3 UCtIUGrT9m9gm1WmjxyA3wHnAoI figure 4a ------- COMMENT: 269782b0ebb602b9 4 Q4IVfM8U9VYmL1s29MkANRIe0zE pulse labeling revealed no defects in mitochondrial translation upon Trm1 deletion (Figure S1) ------- COMMENT: 269782b0ebb602b9 9 H2vppWPoyx2OE1rkRnNitp2y+gU Trm1 robustly modified nuclear- and mitochondrial-encoded tRNAs, consistent with its proposed localization to the nucleus and mitochondria, while M1 Trm1 only modified mitochondrially- encoded tRNAs Certain nuclear-encoded tRNAs, including tRNA SerUGA and tRNA LeuAAG were robustly modified by endogenous Trm1 (Figure 1D, “wild type”, lane 1) and overexpressed M24 Trm1 (Figure 1D, lane 3) ------- COMMENT: 269782b0ebb602b9 10 ztR0AhhWeGY9ijOJE/XqKcCZb9A the D201A mutant showed the same lack of modification of nuclear- and mitochondrial-encoded tRNAs as trm1∆ cells transformed with an empty vector, despite similar levels of protein accumulation as the wild type overexpressed isoform (Figure 1D). ------- COMMENT: 269782b0ebb602b9 11 ztR0AhhWeGY9ijOJE/XqKcCZb9A the D201A mutant showed the same lack of modification of nuclear- and mitochondrial-encoded tRNAs as trm1∆ cells transformed with an empty vector, despite similar levels of protein accumulation as the wild type overexpressed isoform (Figure 1D). ------- COMMENT: 269782b0ebb602b9 12 44H1mSJdRWAmvvcwrXnDT5AKqLI Wild type and D201A exhibited comparable binding affinity, suggesting that disruption of the putative catalytic site does not impair tRNA binding affinity or binding cooperativity (Figure 2A, B, Figure S2, Table S5). ------- COMMENT: 269782b0ebb602b9 13 0EjgySjwgs9Hc6IowJxeB3KOWbc (comment: normal stop codon readthrough) ------- COMMENT: 269782b0ebb602b9 14 6cOJKYDA8Cww+MaGjazvvffRX9s We found that both wild type and catalytically inactive nuclear-targeted (M24) Trm1 promoted suppression of the tRNA SerUCA allele in a sla1∆ background, suggesting that modification is not strictly required for suppression activity and that Trm1 promotes pre-tRNA maturation even in the absence of catalysis (Figure 3A, B). ------- COMMENT: 269782b0ebb602b9 16 rG/KytK/cRAjMIq1P2g2+ZwPTVc “These northern blots supported the expected subcellular targeting of the Trm1 isoforms: M24 Trm1 robustly modified nuclear- and mitochondrial‑encoded tRNAs, consistent with its proposed localization to the nucleus and mitochondria, while M1 Trm1 only modified mitochondrially‑encoded tRNAs, suggesting that it is solely present in the mitochondria. (Fig. 1 D). ------- COMMENT: 269782b0ebb602b9 17 rG/KytK/cRAjMIq1P2g2+ZwPTVc “These northern blots supported the expected subcellular targeting of the Trm1 isoforms: M24 Trm1 robustly modified nuclear- and mitochondrial‑encoded tRNAs, consistent with its proposed localization to the nucleus and mitochondria, while M1 Trm1 only modified mitochondrially‑encoded tRNAs, suggesting that it is solely present in the mitochondria. (Fig. 1 D). ------- COMMENT: 269782b0ebb602b9 18 rG/KytK/cRAjMIq1P2g2+ZwPTVc “These northern blots supported the expected subcellular targeting of the Trm1 isoforms: M24 Trm1 robustly modified nuclear- and mitochondrial‑encoded tRNAs, consistent with its proposed localization to the nucleus and mitochondria, while M1 Trm1 only modified mitochondrially‑encoded tRNAs, suggesting that it is solely present in the mitochondria. (Fig. 1 D). ------- COMMENT: 26a7509190100074 2 SMJ+dJ0jvSMhIyIBGFXZrYZkDm0 fig 1 b ------- COMMENT: 26a7509190100074 3 V8aY9cMSuflWPDnZXK1Ej8D/EEk fig 1b ------- COMMENT: 26a7509190100074 104 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 26a7509190100074 111 +ELLjwUfK5aTh8y7xc8m451gJk4 Fig 1 C ------- COMMENT: 26a7509190100074 112 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 26a7509190100074 114 LQmuKZbL9ND56pzzWctDT58tWRE Figure 3C ------- COMMENT: 26a7509190100074 116 8zdno63XFs3BFsVP161LRtfCMNM Figure 3D ------- COMMENT: 26a7509190100074 117 LQmuKZbL9ND56pzzWctDT58tWRE Figure 3C ------- COMMENT: 26a7509190100074 118 qRuiFIUfCEaRK2U9+oSKa18znxI Figure 3E ------- COMMENT: 26c28361072dd950 4 K7Wxa+xZmmLRpbHvZzcriqLCzSY throughout cell cycle; present in approximately equal stoichiometry with Alp4/GCP2 (immunoblotting and quantification of GFP-Mzt1 and GFP-Alp4 signals at the SPB) ------- COMMENT: 26c28361072dd950 85 K7Wxa+xZmmLRpbHvZzcriqLCzSY throughout cell cycle; present in approximately equal stoichiometry with Alp4/GCP2 (immunoblotting and quantification of GFP-Mzt1 and GFP-Alp4 signals at the SPB) ------- COMMENT: 270b9bbbfc56bc72 1 8+uejd6714RPHZrTsS/berN/LZc This allele removes motif B' through E in reverse transcriptase domain of telomerase catalytic subunit. This allele showed similar rate of telomere shortening as trt1∆::his3+ allele that remove 99% of ORF. ------- COMMENT: 270b9bbbfc56bc72 2 muEmWTAzcH9CozZCAz9Opld4/LI Cells show progressive telomere shortening. ------- COMMENT: 270b9bbbfc56bc72 3 KU9xSBfVSTHZbYVBuJPha9QU01g This allele removes motif B' through E in reverse transcriptase domain of telomerase catalytic subunit. This allele showed similar delayed grow defect phenotype as trt1∆::his3+ allele that remove 99% of ORF. ------- COMMENT: 270b9bbbfc56bc72 4 SoTKRHkx1c5+64oIiXU9gT+Euxw This allele removes motif B' through E in reverse transcriptase domain of telomerase catalytic subunit. This allele showed similar extent of delayed cell elongation phenotype as trt1∆::his3+ allele that remove 99% of ORF. ------- COMMENT: 270b9bbbfc56bc72 5 UOWrx6BAqDfn24s7YuGxWj2DbDM Cell growth rate is reduced in later generation due to telomere shortening. In early generation, cells growth rate is not distingushiable from trt1+ cells. ------- COMMENT: 270b9bbbfc56bc72 6 hb8y8jwDCtBRBv2r7fkx9p0tYoc Cells look normal in early generation, but show many elongated cells in later generation due to telomere shortening. ------- COMMENT: 2725f4d76602a0b1 7 1R7eySkT0xA4FHFHHg714pDN1jw ------- COMMENT: 273401965d727d19 1 YYgzGAUr7wJgZmsQ2/iXYW6vcCI (comment: CHECK during premeiotic DNA replication) ------- COMMENT: 27524319ad84c25c 4 oJuEeAJlLFGjglwQzHPioGt6w0Y Table 4 (comment: suppressor of cdc25-22) ------- COMMENT: 27524319ad84c25c 5 oJuEeAJlLFGjglwQzHPioGt6w0Y Table 4 (comment: suppressor of cdc25-22) ------- COMMENT: 27524319ad84c25c 6 QeuG3UhaHTxVEbVj3G0WwJMXKso Table 3 (comment: suppressor of cdc25-22) ------- COMMENT: 27524319ad84c25c 7 pm4h0lv0qODSS64sbkh0YVJQiNQ (comment: the restrictive temperature for a cdc25-22 diploid is 32°C) ------- COMMENT: 27524319ad84c25c 8 bVVGEeN5IkCWIvAjwFpEe+eJuTM (comment: cells heterozygous for stf1-1 are more elongated that stf1-1 homozygous cells) ------- COMMENT: 27524319ad84c25c 9 ngBunqpDxsw+bE2XlOlIf03u/p4 (comment: cells heterozygous for stf1-1 form small colonies at restrictive temperature ~20-200 cells) ------- COMMENT: 27524319ad84c25c 10 jh1+kuKzs4x1VRjLXN/bAOV5Z4U (comment: cells homozygous for stf1-1 are not as elongated as stf1-1 heterozygous cells at restrictive temperature) ------- COMMENT: 27524319ad84c25c 11 +Fnn1IsRRyB+/gjho8M9NtO56GM (comment: cells homozygous for stf1-1 form small colonies at restrictive temperature ~20-200 cells) ------- COMMENT: 27524319ad84c25c 13 gCU78x3S01ZYFb+0gdnMW/EOn+E (comment: stf1-1/stf1-2 cells are not as elongated as stf1-1 heterozygous cells at restrictive temperature) ------- COMMENT: 27524319ad84c25c 14 a8pt5ox3qeGXSFCcUOtmwhNXcgU (comment: stf1-1/stf1-3 cells form small colonies at restrictive temperature ~20-200 cells) ------- COMMENT: 27524319ad84c25c 16 nL1/Os3a+uDj6XTDP90d8R12RV4 Table 4 (comment: stf1-1 is a suppressor of cdc25-M51) ------- COMMENT: 27524319ad84c25c 17 i6o8kCCcc7aITzW1QrCBi8S8cHM Table 4 (comment: suppressor of cdc25-disruption occasional cdc- cells observed) ------- COMMENT: 27524319ad84c25c 18 1uR3ROcjkQKL+Ayfc0GYOB/FBZ0 (comment: stf1-1/stf1-3 cells are not as elongated as stf1-1 heterozygous cells at restrictive temperature) ------- COMMENT: 27524319ad84c25c 19 rPEVwCpvVUMbZFXrjFM4jCtig4w Table 4 ------- COMMENT: 27524319ad84c25c 20 O+XCii/IxbCU9qZk8p978Qkazbw Table 5 (comment: no genetic interaction with stf1-1) ------- COMMENT: 27524319ad84c25c 21 O+XCii/IxbCU9qZk8p978Qkazbw Table 5 (comment: no genetic interaction with stf1-1) ------- COMMENT: 27524319ad84c25c 22 O+XCii/IxbCU9qZk8p978Qkazbw Table 5 (comment: no genetic interaction with stf1-1) ------- COMMENT: 27524319ad84c25c 23 LTOCzjJunIMvpsnHu/3FHW4zSMs Table 5 (comment: no genetic interaction with stf1-1) ------- COMMENT: 27524319ad84c25c 24 0kydOUAVekPPQ8iOkn3P1q12po0 Table 5 (comment: This mutant is a revertant of cdc2-M35) ------- COMMENT: 27524319ad84c25c 25 Vzn70bh0HS6269nZay099gSfVZ0 Table 5 (comment: no genetic interaction with stf1-1 cdc2-59 is a cold sensitive cdc2 mutant cdc at low (25°C) temperature and wee at high temperature (35°C)) ------- COMMENT: 27524319ad84c25c 26 O+XCii/IxbCU9qZk8p978Qkazbw Table 5 (comment: no genetic interaction with stf1-1) ------- COMMENT: 27524319ad84c25c 27 O+XCii/IxbCU9qZk8p978Qkazbw Table 5 (comment: no genetic interaction with stf1-1) ------- COMMENT: 27524319ad84c25c 28 W3b09N097tjLRizBL3gmQTyfF4Q Table 5 (comment: cdc2-3w and stf1-1 have additive effect on cdc25-22 cell size at restrictive temperature) ------- COMMENT: 27524319ad84c25c 30 0PgJqnvJdkJmf5Qjk+HK3H11quk Table 6 (comment: wee1-50 and stf1-1 have an additive effect to suppress cdc25-22 phenotype at the restrictive temperature) ------- COMMENT: 27524319ad84c25c 31 UrdLwJ+onf06bhtpk2VkW6IxJrk Table 6 ------- COMMENT: 27524319ad84c25c 32 +S43NdCOqfDW4hYmr+MmWVO0RS0 Table 7 (comment: Cells are slightly shorter at high temperature when stf1-1 present) ------- COMMENT: 27524319ad84c25c 33 +S43NdCOqfDW4hYmr+MmWVO0RS0 Table 7 (comment: Cells are slightly shorter at high temperature when stf1-1 present) ------- COMMENT: 27524319ad84c25c 34 fSqLQUxYzzBaBaITyUQf5enHE4M (comment: dis2+ over expression reverses the stf1-1 suppression cdc25-22) ------- COMMENT: 27524319ad84c25c 35 1L+WZDxadvGGnA/d2ki0uoIEJ04 Table 5 (comment: cdc2-1w and stf1-1 have additive effect on cdc25-22 cell size at restrictive temperature) ------- COMMENT: 27524319ad84c25c 36 a/5E/fmEBzkVzFJ0Na7+kWW8B08 Table 5 (comment: cdc2-1w rescues cdc25-22 but cells are long) ------- COMMENT: 27524319ad84c25c 37 PO0Xc5E9Mu23vnlTbNN1aqKkec4 Table 5 ------- COMMENT: 27524319ad84c25c 38 o4Wc/ULeuFpjrl+5YrfXXVgWuGA Table 8 (comment: no genetic interaction with stf1-1) ------- COMMENT: 27524319ad84c25c 39 o4Wc/ULeuFpjrl+5YrfXXVgWuGA Table 8 (comment: no genetic interaction with stf1-1) ------- COMMENT: 27524319ad84c25c 40 i6o8kCCcc7aITzW1QrCBi8S8cHM Table 4 (comment: suppressor of cdc25-disruption occasional cdc- cells observed) ------- COMMENT: 27524319ad84c25c 41 nL1/Os3a+uDj6XTDP90d8R12RV4 Table 4 (comment: stf1-1 is a suppressor of cdc25-M51) ------- COMMENT: 27524319ad84c25c 42 oJuEeAJlLFGjglwQzHPioGt6w0Y Table 4 (comment: suppressor of cdc25-22) ------- COMMENT: 27524319ad84c25c 43 oJuEeAJlLFGjglwQzHPioGt6w0Y Table 4 (comment: suppressor of cdc25-22) ------- COMMENT: 27524319ad84c25c 44 FRR/slAnG5dovOwHYEz8gjCd8mU (comment: same phenotype as cells homozygous for stf1-1) ------- COMMENT: 27524319ad84c25c 45 O5I6hzju8uY0rhqI9ezJOVxHnss (comment: same phenotype as cells homozygous for stf1-1) ------- COMMENT: 27524319ad84c25c 46 rPEVwCpvVUMbZFXrjFM4jCtig4w Table 4 ------- COMMENT: 27524319ad84c25c 47 O+XCii/IxbCU9qZk8p978Qkazbw Table 5 no genetic interaction with stf1-1 ------- COMMENT: 27524319ad84c25c 48 O+XCii/IxbCU9qZk8p978Qkazbw (comment: Table 5 no genetic interaction with stf1-1) ------- COMMENT: 27524319ad84c25c 52 0kydOUAVekPPQ8iOkn3P1q12po0 (comment: Table 5 This mutant is a revertant of cdc2-M35) ------- COMMENT: 27553d3352ec591e 1 6+8ePgm+wRwcJ55Ojh2bMV+a47E Of the eight tested mutants, only epe1D consistently formed red-pink colonies on +AHT plates, indicating that 4xtetO-ade6+ can remain repressed without bound TetR-Clr4* (Fig. 4A and figs. S5 and S6). ------- COMMENT: 27553d3352ec591e 2 Z4eWbu21/RJganIJOAJXzyhU4Ig Catalytically inactivating mutations in the Fe(II) or 2-oxyglutarate binding sites of the Epe1 putative demethylase (epe1-H297A and epe1-K314A) had a similar phenotype (Fig. 4A, fig. S6, and table S3) ------- COMMENT: 27553d3352ec591e 3 Z4eWbu21/RJganIJOAJXzyhU4Ig Catalytically inactivating mutations in the Fe(II) or 2-oxyglutarate binding sites of the Epe1 putative demethylase (epe1-H297A and epe1-K314A) had a similar phenotype (Fig. 4A, fig. S6, and table S3) ------- COMMENT: 27553d3352ec591e 4 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 27553d3352ec591e 5 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 27553d3352ec591e 6 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 27553d3352ec591e 7 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 27553d3352ec591e 8 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 27553d3352ec591e 9 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 27553d3352ec591e 10 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 27553d3352ec591e 11 R3ET9gJDNVjKuTqwsQ2uoYzFA1I The analyses presented here are consistent with Epe1 normally acting as an H3K9 demethylase that removes H3K9 methylation from ectopic sites of heterochromatin formation. ------- COMMENT: 277a64012366b14f 15 ZAT0hsdUBE6Y5e26G20G9akTLO8 (comment: non-flocculating cells) ------- COMMENT: 277ac92c6cd07f54 3 eNykCME+Y/RSCZnl18Iq6FEjg1o (comment: constitutive cdc18+ expression) ------- COMMENT: 277ac92c6cd07f54 4 eNykCME+Y/RSCZnl18Iq6FEjg1o (comment: constitutive cdc18+ expression) ------- COMMENT: 277ac92c6cd07f54 5 eNykCME+Y/RSCZnl18Iq6FEjg1o (comment: constitutive cdc18+ expression) ------- COMMENT: 277ac92c6cd07f54 16 XI7XMX4nLVbF4HNoQyXBT0ZoIy4 (comment: inferred from combination of phenotype shown in this paper with background knowledge) ------- COMMENT: 278fd9a9a5dcfc0a 1 IWBngsZeRMrHr7uQl32Lb4IUDnY ------- COMMENT: 279efabf0d924bdf 7 mPzueC8kvcLbWmGGcmG0cUCIvoA (comment: localization requires microtubules (assayed using thiabendazole or carbendazim) but not F-actin (assayed using latrunculin A)" ------- COMMENT: 27b51a588fa236c6 3 HjH/SYuT8l6CtaULRnKmRc3496w Figures 1A and 2 ------- COMMENT: 27b51a588fa236c6 4 HjH/SYuT8l6CtaULRnKmRc3496w Figures 1A and 2 ------- COMMENT: 27b51a588fa236c6 8 Q0RkHJgJjR4UPXKqNU6MnAfV8ZI Figure 1B ------- COMMENT: 27b51a588fa236c6 9 Q0RkHJgJjR4UPXKqNU6MnAfV8ZI Figure 1B ------- COMMENT: 27b51a588fa236c6 10 Q0RkHJgJjR4UPXKqNU6MnAfV8ZI Figure 1B ------- COMMENT: 27b51a588fa236c6 11 HjH/SYuT8l6CtaULRnKmRc3496w Figures 1A and 2 ------- COMMENT: 27b51a588fa236c6 12 HjH/SYuT8l6CtaULRnKmRc3496w Figures 1A and 2 ------- COMMENT: 27b51a588fa236c6 13 rBO7tkMyhLnFZ1UGqQQau/maZwc By metaphase I, 100% of cells (n 􏰆 100) contained a strong nuclear signal. Sgo1-GFP was concentrated in distinct foci in about half of these metaphase I cells (Figure 3A-2). ------- COMMENT: 27b51a588fa236c6 22 FDaCf/mAFZylwqY5bwzCzIpiKHU Furthermore, no Rec8-specific fluorescence at all could be detected in 10% of the mutant binucleates. In contrast, the distribution of Rec8- GFP fluorescence in 􏰀sgo2 mutant binucleates was in- distinguishable from wild-type. ------- COMMENT: 27bd8acbaa46a888 60 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: 27e00ac6f1e1dbac 2 EFW2ZyPrsohsIlA8ktLI4F/1NRM (comment: CHECK Pck2) ------- COMMENT: 281e81f28d87cf76 18 jnn4hnR7HOITkOBgIL8ijgnKjpc Supp fig7 ------- COMMENT: 281e81f28d87cf76 19 jFcSj3Abmq6oxn/JkLjWEWlf9C0 Supp fig7 ------- COMMENT: 281e81f28d87cf76 20 jFcSj3Abmq6oxn/JkLjWEWlf9C0 Supp fig7 ------- COMMENT: 28333b01f58bc586 3 IEpYGoTh9CnFFc8ey7oOElkYz2M Figure 4, A and B ------- COMMENT: 28333b01f58bc586 7 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 28333b01f58bc586 12 RrRjX3OLwidVAH5kdMSPk+UxGAk Although there was a small difference in CR formation between pxl1(9A) (13.6 +/- 2.5; 33 cells) and pxl1(9D) (12.2 +/- 2.3 min; 41 cells), formation was similar in pxl1(9D) and pxl1+ (12.4 +/- 3.0; 32 cells), and there were no significant differences in the durations of CR maturation. ------- COMMENT: 28333b01f58bc586 13 RrRjX3OLwidVAH5kdMSPk+UxGAk Although there was a small difference in CR formation between pxl1(9A) (13.6 +/- 2.5; 33 cells) and pxl1(9D) (12.2 +/- 2.3 min; 41 cells), formation was similar in pxl1(9D) and pxl1+ (12.4 +/- 3.0; 32 cells), and there were no significant differences in the durations of CR maturation. ------- COMMENT: 28333b01f58bc586 14 axxkHCjbslSE61u5pZ1vgROlSTY Constriction took longer in pxl1(9A) ------- COMMENT: 28333b01f58bc586 15 ausrlfzIzDdjSMRan74Reps0oc8 Cdk1 phosphorylation of Pxl1 reduced binding to the F-BAR domain of Cdc15 (Figure 5A), but not to Cdc15C (Figure 5B). ------- COMMENT: 28333b01f58bc586 18 jh29Rx3ogLUZ9E7ynd+SO8lIM7U (comment: although not IDA, there is experimental data to support this inference) ------- COMMENT: 28418babe512a732 1 H2jIMJcIJfNwXtuDKACzGAHQdyE Figure 1E, F. The average constriction rate of Δmid1 contractile rings is 0.27 μm/min (Saha and Pollard, 2012a), but the distribution of constriction rates appears bimodal with fast and slow subpopulations. The type of strand that builds the contractile ring strongly correlates with its constriction rate, with contractile rings made from nascent strands constricting faster (0.32 μm/min) and contractile rings made from enduring strands constricting more slowly (0.20 μm/min; Fig. 1 E). ------- COMMENT: 28418babe512a732 2 u/Caj2JhQcQkxoCA0FjZlVPXNL4 Figure 1A, B ------- COMMENT: 28418babe512a732 3 ryme/WWmvu/jdStFuBYbgAMj2xk Figure 2 Figure 3 Figure 4 Figure S2 ------- COMMENT: 28418babe512a732 4 RkhnxXQPJ8UtoLFiSTNCF5garLo Figure 3 Figure 4 Figure S2 ------- COMMENT: 28418babe512a732 6 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 28418babe512a732 7 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 28418babe512a732 8 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 28418babe512a732 9 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 28418babe512a732 10 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 28418babe512a732 11 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 28418babe512a732 12 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 28418babe512a732 13 wA0QnjyEYblGx6M1JY0aH2l5eT4 Figure 1E, F. We generated Δmid1 Δmyp2 double-mutant cells and found that the distribution of constriction rates of their contractile rings is still bimodal, albeit with both populations constricting 25–50% more slowly than the Δmid1 populations, consistent with Myp2p being responsible for ∼50% of the constriction rate ------- COMMENT: 28418babe512a732 14 2cORd/C9kj1IO/J6odmucBxqWag Figure 1E, F ------- COMMENT: 28418babe512a732 15 cBkoQeNUO2nhvqsQv9e+I0zwm5M Figure 5 (comment: vw: changed severity from high to low as this seems to partially rescue mid1-delta?) ------- COMMENT: 28418babe512a732 16 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 28418babe512a732 17 NwtsRR535/Ff4JMWE6sr44Rqvy4 Figure 1, Figure 5. +5 min, a 15-min delay compared with wild-type cells ------- COMMENT: 28418babe512a732 18 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 28418babe512a732 19 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 28418babe512a732 20 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 28418babe512a732 21 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 28418babe512a732 22 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 28418babe512a732 23 lxfcfhMlMFhArb1Bu79b0cRYNi0 Figure 1E, F. (comment: VW instead of abnormal, I did increased and decreased rate with low penetrance i.e the rate is variable within the population, someincreased and some decrreased, although there see to be 2 distinct sub-populations we can't capture this effectively) ------- COMMENT: 28418babe512a732 24 +4qAOtBoSFHGY1kauq1JARbhNFk (Fig. 4 C). Cdc12p distributed in a smaller zone in the R-nodes of Δmid1 cells...node dimensions in the R-nodes of constricting contractile rings..... ------- COMMENT: 288cc0705ff9b896 64 gTJdaHQ1EfmefmrvMVF/U3PcxTs Crb2 binds phosphorylated histone H2A (Hta1 Serine-129 and Hta2 Serine-128) through its C-terminal BRCT domains ------- COMMENT: 288cc0705ff9b896 68 gTJdaHQ1EfmefmrvMVF/U3PcxTs Crb2 binds phosphorylated histone H2A (Hta1 Serine-129 and Hta2 Serine-128) through its C-terminal BRCT domains ------- COMMENT: 288cc0705ff9b896 73 gTJdaHQ1EfmefmrvMVF/U3PcxTs Crb2 binds phosphorylated histone H2A (Hta1 Serine-129 and Hta2 Serine-128) through its C-terminal BRCT domains ------- COMMENT: 288cc0705ff9b896 74 gTJdaHQ1EfmefmrvMVF/U3PcxTs Crb2 binds phosphorylated histone H2A (Hta1 Serine-129 and Hta2 Serine-128) through its C-terminal BRCT domains ------- COMMENT: 28977e64fba675ea 1 45xLKr2zrkeXN6C8YPpBCvO7Fgc Mtl2p-GFP showed an even membrane distribution with little intra- cellular signals. ------- COMMENT: 28977e64fba675ea 2 KP0dR3CEbV5nGj5pHyIt5NoUab8 Wsc1p-GFP was found along the entire plasma membrane, but appeared much more concen- trated in patches at the cell ends. We also noted that Wsc1p-GFP accumulated in intracellular compartments (Fig. 3C and D). ------- COMMENT: 28977e64fba675ea 3 qCPP2TOyl9WTWEtjO9tinzBT9fA The wsc1D and mtl2D mutants grew well under standard growth conditions at both 28 and 37°C and entered the stationary phase at the same time as the wild-type cul- tures. ------- COMMENT: 28977e64fba675ea 4 qCPP2TOyl9WTWEtjO9tinzBT9fA The wsc1D and mtl2D mutants grew well under standard growth conditions at both 28 and 37°C and entered the stationary phase at the same time as the wild-type cul- tures. ------- COMMENT: 28977e64fba675ea 5 teT0DvQUtNWlVkmZg9+FyFXZTso wsc1D and mtl2D cells did not exhibit any evident morphological changes (Fig. 1B) ------- COMMENT: 28977e64fba675ea 6 teT0DvQUtNWlVkmZg9+FyFXZTso wsc1D and mtl2D cells did not exhibit any evident morphological changes (Fig. 1B) ------- COMMENT: 28977e64fba675ea 7 M65dWrf1LNftAEMilN5cfrcfTac ~8% of the cells in the wsc1D mutant and 15% of the cells in mtl2D were lysed (Fig. 1B) ------- COMMENT: 28977e64fba675ea 8 M65dWrf1LNftAEMilN5cfrcfTac ~8% of the cells in the wsc1D mutant and 15% of the cells in mtl2D were lysed (Fig. 1B) ------- COMMENT: 28977e64fba675ea 9 DrDCirE7ZFohA31i5d+bXJLfPg4 mtl2D cells were unable to grow on plates sup- plemented with 0.5 lg/mL Csp, whereas the wild-type cells were able to withstand concentrations of up to 5 lg/ mL ------- COMMENT: 28977e64fba675ea 10 raotX86ApgsRPpXoElbTcL3o9SU wsc1D cell growth was inhibited above 2 lg/mL of Csp (Fig. 1C) ------- COMMENT: 28977e64fba675ea 11 OrJAZ36X/Lo4zucry9XMMuvkBmo We found that GS activity was slightly reduced in mtl2D null cells (Fig. 1D) ------- COMMENT: 28977e64fba675ea 12 CPwArkHBym9lgZhc1dArnniZrBs Moreover, when we looked at the cell wall composition of mtl2D mutants we found a decrease in the total amount of glucose incorpo- rated in the cell wall as compared with wild-type cells (30% in wild-type cells and 25% in mtl2D). The differ- ence was mainly due to a decrease in the b–glucan content (17% in the wild type and 13% in the mtl2D) (Fig. 1E). ------- COMMENT: 28977e64fba675ea 13 z89VG+d+PzfzkGOdpy8yAe8rJX0 Deletion of mtl2+ rendered cells hypersensitive to caffeine, vanadate, NaCl, H2O2, and SDS (see Fig. S1). ------- COMMENT: 28977e64fba675ea 14 z89VG+d+PzfzkGOdpy8yAe8rJX0 Deletion of mtl2+ rendered cells hypersensitive to caffeine, vanadate, NaCl, H2O2, and SDS (see Fig. S1). ------- COMMENT: 28977e64fba675ea 15 z89VG+d+PzfzkGOdpy8yAe8rJX0 Deletion of mtl2+ rendered cells hypersensitive to caffeine, vanadate, NaCl, H2O2, and SDS (see Fig. S1). ------- COMMENT: 28977e64fba675ea 16 z89VG+d+PzfzkGOdpy8yAe8rJX0 Deletion of mtl2+ rendered cells hypersensitive to caffeine, vanadate, NaCl, H2O2, and SDS (see Fig. S1). ------- COMMENT: 28977e64fba675ea 17 z89VG+d+PzfzkGOdpy8yAe8rJX0 Deletion of mtl2+ rendered cells hypersensitive to caffeine, vanadate, NaCl, H2O2, and SDS (see Fig. S1). ------- COMMENT: 28977e64fba675ea 18 C3O5B3+s1iKNnIdTAvAVHCWhyyk The mating rate was not affected in mtl2Dh+ 9 mtl2Dh or wsc1Dh+ 9 wsc1Dh homozygous crosses, ------- COMMENT: 28977e64fba675ea 19 C3O5B3+s1iKNnIdTAvAVHCWhyyk The mating rate was not affected in mtl2Dh+ 9 mtl2Dh or wsc1Dh+ 9 wsc1Dh homozygous crosses, ------- COMMENT: 28977e64fba675ea 21 x4AHE4rUtAAwQ2ueQwhz4uaKGeY Repres- sion of mtl2+ promoted cell lysis and the cells shrunk without the release of cytoplasmic material. ------- COMMENT: 28977e64fba675ea 22 x4AHE4rUtAAwQ2ueQwhz4uaKGeY Repres- sion of mtl2+ promoted cell lysis and the cells shrunk without the release of cytoplasmic material. ------- COMMENT: 28977e64fba675ea 23 KP0dR3CEbV5nGj5pHyIt5NoUab8 Wsc1p-GFP was found along the entire plasma membrane, but appeared much more concen- trated in patches at the cell ends. We also noted that Wsc1p-GFP accumulated in intracellular compartments (Fig. 3C and D). ------- COMMENT: 28977e64fba675ea 24 vCUIJIdf3ZXoVetUW9v3Mjw46o4 In tea4D cells, Wsc1p-GFP localized mainly to the growing tip that was stained with Cfw (Fig. 3C). ------- COMMENT: 28977e64fba675ea 25 DrDCirE7ZFohA31i5d+bXJLfPg4 mtl2D cells were unable to grow on plates sup- plemented with 0.5 lg/mL Csp, whereas the wild-type cells were able to withstand concentrations of up to 5 lg/ mL ------- COMMENT: 28977e64fba675ea 26 vpmxZpf9ZKMTiW1zbEkD+ogGFkE (Figure 4A) expression of rho1+, rgf1+, and rgf2+ restored the growth of an mtl2D mutant in the presence of the antifungal agent, whereas overexpression of rgf3+ did not suppress the growth defect. ------- COMMENT: 28977e64fba675ea 27 vpmxZpf9ZKMTiW1zbEkD+ogGFkE (Figure 4A) expression of rho1+, rgf1+, and rgf2+ restored the growth of an mtl2D mutant in the presence of the antifungal agent, whereas overexpression of rgf3+ did not suppress the growth defect. ------- COMMENT: 28977e64fba675ea 28 vpmxZpf9ZKMTiW1zbEkD+ogGFkE (Figure 4A) expression of rho1+, rgf1+, and rgf2+ restored the growth of an mtl2D mutant in the presence of the antifungal agent, whereas overexpression of rgf3+ did not suppress the growth defect. ------- COMMENT: 28977e64fba675ea 29 DrDCirE7ZFohA31i5d+bXJLfPg4 mtl2D cells were unable to grow on plates sup- plemented with 0.5 lg/mL Csp, whereas the wild-type cells were able to withstand concentrations of up to 5 lg/ mL ------- COMMENT: 28977e64fba675ea 30 jvQyzrJymw6gfIsMMzwRxyIj2fY Figure 4B, the activation of Rho1p, through the expres- sion of a constitutively active form of Rho1p or overex- pression of the wild-type Rho1p or Rgf1p, efficiently restored the growth of a strain (P81nmt-mtl2 wsc1D) unable to grow in the presence of thiamine (promoter off). ------- COMMENT: 28977e64fba675ea 31 hQBKh4jrFMMaqkTV3whAbr9ZEfo GTP bound modified form . Interestingly, mtl2D and wsc1D mutants contained much less Rho1p-GTP than wild-type cells when the cultures were grown in the presence of 0.1 lg/mL of Csp for 16 h prior to harvesting (Fig. 4C) ------- COMMENT: 28977e64fba675ea 32 HMW0H1E5U1AzA3jTBUEOlLn+FBg GTP bound modified form. Interest- ingly, mtl2D and wsc1D mutants contained much less Rho1p-GTP than wild-type cells when the cultures were grown in the presence of 0.1 lg/mL of Csp for 16 h prior to harvesting (Fig. 4C) ------- COMMENT: 28977e64fba675ea 33 bZDdUqBCFYkhBJunlYx+Y6wSpoA . Interest- ingly, mtl2D and wsc1D mutants contained much less Rho1p-GTP than wild-type cells when the cultures were grown in the presence of 0.1 lg/mL of Csp for 16 h prior to harvesting (Fig. 4C) ------- COMMENT: 28977e64fba675ea 34 bZDdUqBCFYkhBJunlYx+Y6wSpoA . Interest- ingly, mtl2D and wsc1D mutants contained much less Rho1p-GTP than wild-type cells when the cultures were grown in the presence of 0.1 lg/mL of Csp for 16 h prior to harvesting (Fig. 4C) ------- COMMENT: 28977e64fba675ea 35 bZDdUqBCFYkhBJunlYx+Y6wSpoA . Interest- ingly, mtl2D and wsc1D mutants contained much less Rho1p-GTP than wild-type cells when the cultures were grown in the presence of 0.1 lg/mL of Csp for 16 h prior to harvesting (Fig. 4C) ------- COMMENT: 28977e64fba675ea 36 P21l4dwWHgfyWE8y9Pvr6Ojs2Pg The wsc1Drgf2D double mutant was viable, but we failed to find any double- mutant spore wsc1Drgf1D. T ------- COMMENT: 28977e64fba675ea 37 JlltVvp+Jr+vJ/uOG5SrVH+SuBU (comment: described as rounded , but more 'stubby') ------- COMMENT: 28977e64fba675ea 38 qCPP2TOyl9WTWEtjO9tinzBT9fA The wsc1D and mtl2D mutants grew well under standard growth conditions at both 28 and 37°C and entered the stationary phase at the same time as the wild-type cul- tures. ------- COMMENT: 28bb12c162a98275 9 kr1w3qVhJWywhJTs+w3PyKphtY8 (comment: closest we can get to "at stalled fork" with available terms) ------- COMMENT: 28c85bd1757a57ff 1 X6MW+cFq1HMNd/X3pCVrLEUQnOo Figure S6G ------- COMMENT: 28c85bd1757a57ff 2 6mSmZ7wr9sEybBDCqgtkyhF3MKM Figure 1B, 2D, 5B, 6C, 7B, 7C ------- COMMENT: 28c85bd1757a57ff 3 T5IpnPZOGmRF8eTL8PhSQswvbO4 Figure 1B, 5B ------- COMMENT: 28c85bd1757a57ff 4 T5IpnPZOGmRF8eTL8PhSQswvbO4 Figure 1B, 5B ------- COMMENT: 28c85bd1757a57ff 6 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 28c85bd1757a57ff 7 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 28c85bd1757a57ff 8 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 28c85bd1757a57ff 9 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 28c85bd1757a57ff 10 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 28c85bd1757a57ff 11 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 28c85bd1757a57ff 12 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 28c85bd1757a57ff 13 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 28c85bd1757a57ff 14 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 28c85bd1757a57ff 16 x2s1P5qIgmIWjA8fJIImFc3qCDQ Figure S6D ------- COMMENT: 28c85bd1757a57ff 17 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 28c85bd1757a57ff 18 Ux5vufI1Iu5YcBcUP51/JQRgj18 Figure S6B ------- COMMENT: 28c85bd1757a57ff 19 lyx079axKDEjEKNNPxx1yzTFV7Y Figure 3, S1 ------- COMMENT: 28c85bd1757a57ff 20 lyx079axKDEjEKNNPxx1yzTFV7Y Figure 3, S1 ------- COMMENT: 28c85bd1757a57ff 21 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: 28c85bd1757a57ff 22 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: 28c85bd1757a57ff 23 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 28c85bd1757a57ff 24 s7RZyJrftDoLArvCmQ+0iucl7eY Figure 2A even if securin levels were elevated to only about eight times the wild-type level (Kamenz et al., 2015) (Figure 2A ------- COMMENT: 28c85bd1757a57ff 25 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: 28c85bd1757a57ff 26 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 28c85bd1757a57ff 27 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 28c85bd1757a57ff 28 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 28c85bd1757a57ff 29 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 28c85bd1757a57ff 30 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 28c85bd1757a57ff 31 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 28c85bd1757a57ff 32 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 28c85bd1757a57ff 33 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 28c85bd1757a57ff 34 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 28c85bd1757a57ff 35 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 28c85bd1757a57ff 36 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 28c85bd1757a57ff 37 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 28c85bd1757a57ff 38 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 28c85bd1757a57ff 39 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 28c85bd1757a57ff 40 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 28c85bd1757a57ff 41 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 28c85bd1757a57ff 42 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 28c85bd1757a57ff 43 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 28c85bd1757a57ff 46 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 28c85bd1757a57ff 47 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 28c85bd1757a57ff 48 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 28c85bd1757a57ff 49 sRbiU/jtYRJxVkHoyWOhqsXRQaU Figure S5 ------- COMMENT: 28c85bd1757a57ff 50 sRbiU/jtYRJxVkHoyWOhqsXRQaU Figure S5 ------- COMMENT: 28c85bd1757a57ff 51 E4rYcahCrgwRxAsYYVHY/2vQNVQ Figure S7 ------- COMMENT: 28c85bd1757a57ff 52 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 28c85bd1757a57ff 53 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 28c85bd1757a57ff 54 CBoHPi8p/frZ9X8f4qdg/md0W3A Consistent with the low levels of separase, we found that sister chromatid separation was delayed in cdc48- 353 mutant cells relative to the decline in CDK1 activity at mitotic exit (Figure 1C). ------- COMMENT: 28c85bd1757a57ff 55 1+Q/05AizRmzJ1CFn0JprCID0f0 figure 3a However, the cellular degradation kinetics of securin-GFP were indistinguishable in cdc48+ and cdc48-353 mutant cells after normalizing for the reduced level (Figure 3A), suggesting that se- curin degradation was unaffected in the cdc48-353 mutant. ------- COMMENT: 28c85bd1757a57ff 56 G//AHrxJYSl6A2+eo/l4lqeSvNA Figure 3a Similar results were obtained for Cdc13-GFP (Figure S1). Hence securin and Cdc13 are still efficiently targeted for proteasomal degradation in the cdc48-353 mutant. ------- COMMENT: 28cd0018d09ab78f 10 h09KQpN7u/q6m3yDchMr2e10lc8 Fig4 ------- COMMENT: 28cd0018d09ab78f 12 3EfvjaMCp77T+8C8sptTG84+fIc Fig. 1B ------- COMMENT: 28cd0018d09ab78f 13 3EfvjaMCp77T+8C8sptTG84+fIc Fig. 1B ------- COMMENT: 28d952f6d70d5b8f 1 +7fPpLIiAo6zQkpN7JfKKA/o3rw in vitro assay ------- COMMENT: 28d952f6d70d5b8f 19 wzccfYXzgCAoUZxpHMLUQr1y3kA Supp S5 ------- COMMENT: 28d952f6d70d5b8f 20 wzccfYXzgCAoUZxpHMLUQr1y3kA Supp S5 ------- COMMENT: 28d952f6d70d5b8f 21 wzccfYXzgCAoUZxpHMLUQr1y3kA Supp S5 ------- COMMENT: 28d952f6d70d5b8f 22 wzccfYXzgCAoUZxpHMLUQr1y3kA Supp S5 ------- COMMENT: 28d952f6d70d5b8f 42 1ZIj7sTV5JedIsZiW+5aD8BJGeQ First, we have shown that a true APC/C substrate regulates the activity of the APC/C. Cells might precisely control protein levels of each or a subset of APC/C substrate to fine-tune the APC/C itself....Mes1 transcripts and protein levels peak in late MI (Izawa et al., 2005; Mata et al., 2002) when Mes1 seques- ters the Fizzy family of proteins to inhibit APC/C, in turn slowing down cyclin B proteolysis (‘‘APC/C inhibited’’ in Figure 4G). At the same time, Mes1 is inhibited through ubiquitylation by APC/C to allow partial APC/C activation required for anaphase I onset. ------- COMMENT: 28dc33fbb04ba432 1 hcMmQUvoetenThMOgyvqPsaeldA (comment: 2M glucose = 36% w/v = A LOT, so it is osmolarity rather than glucose itself I guess) ------- COMMENT: 28dc33fbb04ba432 2 hcMmQUvoetenThMOgyvqPsaeldA (comment: 2M glucose = 36% w/v = A LOT, so it is osmolarity rather than glucose itself I guess) ------- COMMENT: 2911e7e541770e14 1 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 2911e7e541770e14 2 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 2911e7e541770e14 4 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 2911e7e541770e14 5 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 2911e7e541770e14 6 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 2911e7e541770e14 7 9pO0LJxIWAw7ZssJml/5NDBM0go Fig. 3 (comment: CHECK minor rescue) ------- COMMENT: 2911e7e541770e14 8 9pO0LJxIWAw7ZssJml/5NDBM0go Fig. 3 (comment: CHECK minor rescue) ------- COMMENT: 2911e7e541770e14 9 CJJd8K/fCJo9Qz7WTn0zxjFngOo Fig. 4 (comment: CHECK minor rescue) ------- COMMENT: 291d614ef0c90bc4 1 /f3yaXO8s3WDq4pJif4pVu1jzJk ppa2-6 is cold sensitive and undergoes only a few divisions after a shift to 19 (Table S1, Figure 1, A and B). ------- COMMENT: 291d614ef0c90bc4 2 /f3yaXO8s3WDq4pJif4pVu1jzJk ppa2-6 is cold sensitive and undergoes only a few divisions after a shift to 19 (Table S1, Figure 1, A and B). ------- COMMENT: 291d614ef0c90bc4 3 InyVE7brmY4EDotk36MQOCzkOxg The double mutant ppa2-6 ppa1–D was synthetically lethal (Table 4), even when tetrads were dissected at 36 and 32, the permissive temperature for ppa2-6. ------- COMMENT: 291d614ef0c90bc4 4 InyVE7brmY4EDotk36MQOCzkOxg The double mutant ppa2-6 ppa1–D was synthetically lethal (Table 4), even when tetrads were dissected at 36 and 32, the permissive temperature for ppa2-6. ------- COMMENT: 291d614ef0c90bc4 5 /dbWsD2/BLp1ZwlFrF6EbafVz+o Figure 1 A ------- COMMENT: 291d614ef0c90bc4 6 /dbWsD2/BLp1ZwlFrF6EbafVz+o Figure 1 A ------- COMMENT: 291d614ef0c90bc4 7 /dbWsD2/BLp1ZwlFrF6EbafVz+o Figure 1 A ------- COMMENT: 291d614ef0c90bc4 8 /dbWsD2/BLp1ZwlFrF6EbafVz+o Figure 1 A ------- COMMENT: 291d614ef0c90bc4 9 i2Pnk1OVO4dL1/NuE1kiZwjW5t4 Both the GFP-Ypa1p and GFP-Ypa2p proteins showed a uniform cyto- plasmic localization and a faint nuclear signal. ------- COMMENT: 291d614ef0c90bc4 10 i2Pnk1OVO4dL1/NuE1kiZwjW5t4 Both the GFP-Ypa1p and GFP-Ypa2p proteins showed a uniform cyto- plasmic localization and a faint nuclear signal. ------- COMMENT: 291d614ef0c90bc4 11 GyyNdOHRLJxpJIYoCgv4GtL0sJE Measurement of ypa2–D cells revealed that they divide at a reduced cell length at both the permissive and restrictive temperatures, similar to ppa2–D (Table 2). ------- COMMENT: 291d614ef0c90bc4 12 ajtRwWP5fftoDh9lu4+05+IhbIU The double mutant wee1-50 ypa2–D was syntheti- cally lethal, with the germinating spore giving rise to one or two small, rounded cells at 29 (DNS) ------- COMMENT: 291d614ef0c90bc4 13 0GYRl4emYXSULlPATSddkTESvek The double mutant wee1-50 ypa2–D was syntheti- cally lethal, with the germinating spore giving rise to one or two small, rounded cells at 29 (DNS) ------- COMMENT: 291d614ef0c90bc4 14 owoQMOffrXW5Zizu2tAmAcvkgAY Table 3 ------- COMMENT: 291d614ef0c90bc4 15 owoQMOffrXW5Zizu2tAmAcvkgAY Table 3 ------- COMMENT: 291d614ef0c90bc4 16 owoQMOffrXW5Zizu2tAmAcvkgAY Table 3 ------- COMMENT: 291d614ef0c90bc4 17 owoQMOffrXW5Zizu2tAmAcvkgAY Table 3 ------- COMMENT: 291d614ef0c90bc4 18 owoQMOffrXW5Zizu2tAmAcvkgAY Table 3 ------- COMMENT: 291d614ef0c90bc4 19 owoQMOffrXW5Zizu2tAmAcvkgAY Table 3 ------- COMMENT: 291d614ef0c90bc4 20 2He3fSKvXpS3YzqmKt6gbioa8J8 Table 2 ------- COMMENT: 291d614ef0c90bc4 21 2He3fSKvXpS3YzqmKt6gbioa8J8 Table 2 ------- COMMENT: 291d614ef0c90bc4 22 ixRkOLFohv02Tg0l7oDklz6nMyQ Figure 4b ------- COMMENT: 291d614ef0c90bc4 23 ixRkOLFohv02Tg0l7oDklz6nMyQ Figure 4b ------- COMMENT: 291d614ef0c90bc4 24 ixRkOLFohv02Tg0l7oDklz6nMyQ Figure 4b ------- COMMENT: 291d614ef0c90bc4 25 ixRkOLFohv02Tg0l7oDklz6nMyQ Figure 4b ------- COMMENT: 291d614ef0c90bc4 26 ixRkOLFohv02Tg0l7oDklz6nMyQ Figure 4b ------- COMMENT: 291d614ef0c90bc4 27 ixRkOLFohv02Tg0l7oDklz6nMyQ Figure 4b ------- COMMENT: 291d614ef0c90bc4 28 ixRkOLFohv02Tg0l7oDklz6nMyQ Figure 4b ------- COMMENT: 291d614ef0c90bc4 29 ixRkOLFohv02Tg0l7oDklz6nMyQ Figure 4b ------- COMMENT: 291d614ef0c90bc4 30 ixRkOLFohv02Tg0l7oDklz6nMyQ Figure 4b ------- COMMENT: 291d614ef0c90bc4 31 oNBXCEpLOF4EEIb2EPGVqBqNVBY Together, these data lead us to conclude that Ypa2p is involved in determining the timing of mitotic commitment, establishing cell morphology, positioning of the division site, regulation of the SIN, and in completion of cytokinesis. ------- COMMENT: 291d614ef0c90bc4 32 m73BvxTotxBNhBEwi++FHnq5HQo These data therefore implicate Ypa2p and Ppa2p in establishing SIN protein asymmetry during anaphase. ------- COMMENT: 29300b31506c9419 10 3OK85eCMIL/qz5H7zv2s6SIx10M (comment: length) ------- COMMENT: 29300b31506c9419 24 ru5XmDaHrTtAzPVXv/1ZfLdkikU (comment: transient) ------- COMMENT: 29300b31506c9419 30 kKc2lGN54YPBsx6BgBawwC+4SD8 (comment: is delayed but the delay is reduced compared to the single mutant) ------- COMMENT: 29300b31506c9419 34 4iY6SL9TvI8t/hGgE3h/Wl6YMs8 figure 10 C ------- COMMENT: 29300b31506c9419 35 4iY6SL9TvI8t/hGgE3h/Wl6YMs8 figure 10 C ------- COMMENT: 29300b31506c9419 36 pNulm9JDzdo+WPU/0EquTeJ2gu4 (comment: CHECK ADD MODIFIED FORMS) ------- COMMENT: 296774c7adb28ef3 4 WpWHXfHxatvB8B8wjiRiHa+4VdY (comment: CHECK formaldehyde sensitivity) ------- COMMENT: 296774c7adb28ef3 10 WpWHXfHxatvB8B8wjiRiHa+4VdY (comment: CHECK formaldehyde sensitivity) ------- COMMENT: 296774c7adb28ef3 11 WpWHXfHxatvB8B8wjiRiHa+4VdY (comment: CHECK formaldehyde sensitivity) ------- COMMENT: 296774c7adb28ef3 14 WpWHXfHxatvB8B8wjiRiHa+4VdY (comment: CHECK formaldehyde sensitivity) ------- COMMENT: CHECK 296774c7adb28ef3 15 WpWHXfHxatvB8B8wjiRiHa+4VdY (comment: formaldehyde sensitivity) ------- COMMENT: CHECK 296774c7adb28ef3 16 WpWHXfHxatvB8B8wjiRiHa+4VdY (comment: formaldehyde sensitivity) ------- COMMENT: 296774c7adb28ef3 17 WpWHXfHxatvB8B8wjiRiHa+4VdY (comment: CHECK formaldehyde sensitivity) ------- COMMENT: 296774c7adb28ef3 18 WpWHXfHxatvB8B8wjiRiHa+4VdY (comment: CHECK formaldehyde sensitivity) ------- COMMENT: 296774c7adb28ef3 20 WpWHXfHxatvB8B8wjiRiHa+4VdY (comment: CHECK formaldehyde sensitivity) ------- COMMENT: 296774c7adb28ef3 21 WpWHXfHxatvB8B8wjiRiHa+4VdY comment: CHECK formaldehyde sensitivity) ------- COMMENT: 296774c7adb28ef3 22 WpWHXfHxatvB8B8wjiRiHa+4VdY comment: CHECK formaldehyde sensitivity) ------- COMMENT: 296774c7adb28ef3 25 WpWHXfHxatvB8B8wjiRiHa+4VdY (comment: CHECK formaldehyde sensitivity) ------- COMMENT: 296774c7adb28ef3 28 WpWHXfHxatvB8B8wjiRiHa+4VdY (comment: CHECK formaldehyde sensitivity) ------- COMMENT: 296774c7adb28ef3 30 WpWHXfHxatvB8B8wjiRiHa+4VdY (comment: CHECK formaldehyde sensitivity) ------- COMMENT: 296774c7adb28ef3 32 WpWHXfHxatvB8B8wjiRiHa+4VdY (comment: CHECK formaldehyde sensitivity) ------- COMMENT: 296774c7adb28ef3 33 WpWHXfHxatvB8B8wjiRiHa+4VdY (comment: CHECK formaldehyde sensitivity) ------- COMMENT: 296774c7adb28ef3 34 WpWHXfHxatvB8B8wjiRiHa+4VdY (comment: CHECK formaldehyde sensitivity) ------- COMMENT: 296774c7adb28ef3 37 WpWHXfHxatvB8B8wjiRiHa+4VdY (comment: CHECK formaldehyde sensitivity) ------- COMMENT: 296774c7adb28ef3 40 WpWHXfHxatvB8B8wjiRiHa+4VdY (comment: CHECK formaldehyde sensitivity) ------- COMMENT: 296774c7adb28ef3 41 WpWHXfHxatvB8B8wjiRiHa+4VdY (comment: CHECK formaldehyde sensitivity) ------- COMMENT: 296774c7adb28ef3 43 aJjQfpx870H2IpSBeUOAdDfboPo (comment: CHECK camptothecin sensitivity) ------- COMMENT: 296774c7adb28ef3 44 aJjQfpx870H2IpSBeUOAdDfboPo (comment: CHECK camptothecin sensitivity) ------- COMMENT: 296774c7adb28ef3 47 WpWHXfHxatvB8B8wjiRiHa+4VdY (comment: CHECK formaldehyde sensitivity) ------- COMMENT: 296b721850a6d19b 73 XCChH63cWZM9MFIqYT7jGZkccms (comment: inferred from phenotypes and from direct assay using human calcineurin) ------- COMMENT: 297469ade6812e26 1 zKu/QJgQl7tHRHFNYtK4ddRn7HA Figure 6. We found that the dri1∆ cells acquired tolerance to high temperature, as they could form colonies at 39 ◦C, whereas wild-type cells could not (Figure 6C). ------- COMMENT: 297469ade6812e26 9 t4ECKCchOv6pqKvX9EjEn2L8Zfs fig 1c (comment: CHECK dri1 supresses cut7) ------- COMMENT: 297469ade6812e26 10 WIQobKVmOSwF4pnw3zyHiiYk2Lk fig. 1a ------- COMMENT: 297469ade6812e26 13 oXrahwH/EUTq3pU54FPDXMsc++Y fig. 1 ------- COMMENT: 297469ade6812e26 16 m/+wOsBdBxXSvyGK4eoiSMUcJzE Figure 2A,B ------- COMMENT: 297469ade6812e26 17 I35a2EHD0cCnW3iANQE3bjpHdA8 Klp2 levels on spindle microtubules were significantly lower than those in cut7-22 (which are increased compared to WT) ------- COMMENT: 297469ade6812e26 21 Mc1Dl/8ePjG+W3BQIevC1vaEvqw Figure 2D ------- COMMENT: 297469ade6812e26 23 DH59M5NKr7rm30OVwK5Q7e0m+tM (comment: CHECK SHOULD THIS BE NORMAL?) However, the Mal3 protein levels did not change in the presence or absence of Dri1 (Supplementary Figure S3A). Similarly, the levels of Mal3-GFP on the spindle MTs were almost the same both in wild-type and dri1∆ cells (Supplementary Figure S3B). ------- COMMENT: 297469ade6812e26 24 2sa/1a0m4G26mrz2wsYl6H5Ye1w fig S4a ------- COMMENT: 297469ade6812e26 32 RXXXy7UlJSw9821KvCxg1/tJ/2M (comment: CHECK to pac) ------- COMMENT: 297469ade6812e26 33 RXXXy7UlJSw9821KvCxg1/tJ/2M (comment: CHECK to pac) ------- COMMENT: 297469ade6812e26 34 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 297469ade6812e26 36 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 297469ade6812e26 37 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 297469ade6812e26 38 p+oVe0jJEd3iUOZ6+ONJVCdjeM4 Figure 4a ------- COMMENT: 297469ade6812e26 42 I35a2EHD0cCnW3iANQE3bjpHdA8 Klp2 levels on spindle microtubules were significantly lower than those in cut7-22 (which are increased compared to WT) ------- COMMENT: 297469ade6812e26 43 RXXXy7UlJSw9821KvCxg1/tJ/2M (comment: CHECK to pac) ------- COMMENT: 297469ade6812e26 44 RXXXy7UlJSw9821KvCxg1/tJ/2M (comment: CHECK to pac) ------- COMMENT: 297469ade6812e26 45 m/+wOsBdBxXSvyGK4eoiSMUcJzE Figure 2A,B ------- COMMENT: 297469ade6812e26 47 Mc1Dl/8ePjG+W3BQIevC1vaEvqw Figure 2D ------- COMMENT: 297bcc54c0cd9972 1 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 297bcc54c0cd9972 6 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: 297bcc54c0cd9972 7 a11+kMkkTd1yZHK29efTGlJjrFk (Fig. 2A) (Fig. 2B; Fig. S2B). ------- COMMENT: 297bcc54c0cd9972 8 yZYBxZVK4fjMt/auwVgNItrr+6k (comment: CHECK adaptor for dis1-microtubule) ------- COMMENT: 297bcc54c0cd9972 25 21FVt8l00mqiMsA0GyfBPs4gBQE (Fig. 7B,C) ------- COMMENT: 297bcc54c0cd9972 26 SMPbcbCrsx0KWM12A3Msekp9xfs (comment: CHECK dis1-LAPA) (Fig. 7B,C) ------- COMMENT: 297bcc54c0cd9972 28 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 297bcc54c0cd9972 29 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 297bcc54c0cd9972 30 /MgdeBHaT9ABix4/xBezuYbm/z8 (Fig. 7E) ------- COMMENT: 297bcc54c0cd9972 31 ooeqtHIRHIobiRAL89gWS9eZeqc (comment: 2.0% versus <0.1%) (Fig. 7F) ------- COMMENT: 298401f5017b6d28 64 uuG50IaUFbI/MKj+xVHg0iWUFKo (comment: CONDITION 32 degrees) (comment: CHECK mcl1-1 semi-permissive) ------- COMMENT: 298401f5017b6d28 65 uuG50IaUFbI/MKj+xVHg0iWUFKo (comment: CONDITION 32 degrees) (comment: CHECK mcl1-1 semi-permissive) ------- COMMENT: 29d94bfa44bcdf74 1 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 29d94bfa44bcdf74 2 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 29d94bfa44bcdf74 3 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 29d94bfa44bcdf74 4 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 29d94bfa44bcdf74 5 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 29d94bfa44bcdf74 6 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 29d94bfa44bcdf74 7 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 29d94bfa44bcdf74 8 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 29d94bfa44bcdf74 9 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 29d94bfa44bcdf74 10 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 29d94bfa44bcdf74 11 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 29d94bfa44bcdf74 12 ajdfne87YaY/1k5k0Oqi5suprzc Fig. 2C and D ------- COMMENT: 29d94bfa44bcdf74 13 ajdfne87YaY/1k5k0Oqi5suprzc Fig. 2C and D ------- COMMENT: 29d94bfa44bcdf74 14 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 29d94bfa44bcdf74 15 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 29d94bfa44bcdf74 16 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 29d94bfa44bcdf74 17 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 29d94bfa44bcdf74 18 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 29d94bfa44bcdf74 19 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 29d94bfa44bcdf74 20 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 29d94bfa44bcdf74 21 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 29d94bfa44bcdf74 22 4yuIU+HTbhzU3dfOUpicpIlOg2A Fig. 6C and E ------- COMMENT: 29d94bfa44bcdf74 23 /EKJDn1kiFepVVy1Uoj4agCFYLE Fig. 6E ------- COMMENT: 29d94bfa44bcdf74 24 jE2iajuFOR4JEMawZFhqqubAiDY Fig. 6F ------- COMMENT: 29d94bfa44bcdf74 25 jE2iajuFOR4JEMawZFhqqubAiDY Fig. 6F ------- COMMENT: 29d94bfa44bcdf74 26 jE2iajuFOR4JEMawZFhqqubAiDY Fig. 6F ------- COMMENT: 29d94bfa44bcdf74 27 PyRFJS/DvQ+ySvCDquS3Ac9839A The deletion analysis then indicates that the RNA synthesis activity observed in our experiments is intrinsic to Rdp1. Finally, loss of enzymatic activity for the above Rdp1 truncations correlated with the loss of centromeric silencing (Figure 6F). We also examined the activity of an Rdp1 active site point mutation and found that it lacked activity in vitro and silencing function in vivo (T. Sugiyama et al., submitted). Together these results suggest that activity of Rdp1 is required for RNAi-mediated transcriptional gene silencing. ------- COMMENT: 29db59cac09c4779 1 7QL38T9pymNua0mYL9k1ylfEOLU Surprisingly, arginine substitutions of the hydrophobic residues (L101R, L108R, V141R, and V148R) led to degradation and insolubility of mutant proteins of spTbf1TRFH (Figures S3). | Consistently, all L101R, L108R, V141R, and V148R mutations resulted in great degradation of spTbf1 in cells (Figure 2E). ------- COMMENT: 29db59cac09c4779 2 iqbevRvzDhhyo9qiTpEuOT4mP58 Surprisingly, arginine substitutions of the hydrophobic residues (L101R, L108R, V141R, and V148R) led to degradation and insolubility of mutant proteins of spTbf1TRFH (Figures S3) | Consistently, all L101R, L108R, V141R, and V148R mutations resulted in great degradation of spTbf1 in cells (Figure 2E). ------- COMMENT: 29db59cac09c4779 3 iqbevRvzDhhyo9qiTpEuOT4mP58 Surprisingly, arginine substitutions of the hydrophobic residues (L101R, L108R, V141R, and V148R) led to degradation and insolubility of mutant proteins of spTbf1TRFH (Figures S3) | Consistently, all L101R, L108R, V141R, and V148R mutations resulted in great degradation of spTbf1 in cells (Figure 2E). ------- COMMENT: 29db59cac09c4779 4 7QL38T9pymNua0mYL9k1ylfEOLU Surprisingly, arginine substitutions of the hydrophobic residues (L101R, L108R, V141R, and V148R) led to degradation and insolubility of mutant proteins of spTbf1TRFH (Figures S3). | Consistently, all L101R, L108R, V141R, and V148R mutations resulted in great degradation of spTbf1 in cells (Figure 2E). ------- COMMENT: 29db59cac09c4779 5 kR7P3xmDsY8bCJ2PaO+K8FWLesM urthermore, we found that all S100A, T104A, and N107A mutant proteins formed aggregation easily during concentration in our purification process, indicating that these three mutations also resulted in protein instability of spTbf1TRFH, ------- COMMENT: 29db59cac09c4779 6 kR7P3xmDsY8bCJ2PaO+K8FWLesM urthermore, we found that all S100A, T104A, and N107A mutant proteins formed aggregation easily during concentration in our purification process, indicating that these three mutations also resulted in protein instability of spTbf1TRFH, ------- COMMENT: 29db59cac09c4779 7 8L3JSbcmcLAFsGnACODa6BeRvM4 The spTbf1- TeloDNA complex exhibited a significant supershift, consistent with the previous report that spTbf1 binds S. pombe telomeric dsDNA with high affinity (Figure 4F).32 ------- COMMENT: 29db59cac09c4779 8 qpOsY5sUY9lXuryW2a9xnbx7FPo Interestingly, although the internal loop region (spTbf1loop, residues 320– 407) could not bind S. pombe telomeric DNA alone, it enhanced the binding affinity of both spTbf1TRFH and spTbf1Myb-L (Figure 4F), indicative of important roles of spTbf1loop in spTbf1 recognition of telomeric DNA. Consis- tently, deletion of the loop region (spTbf1Dloop) significantly reduced the binding affinity of spTbf1 with S. pombe telomeric DNA (Figure 4F). ------- COMMENT: 2a09c66cd3b7c486 2 TUl9fngQi9T2r1Ci1WAnQSqMOPg fig 1a ------- COMMENT: 2a09c66cd3b7c486 3 TUl9fngQi9T2r1Ci1WAnQSqMOPg fig 1a ------- COMMENT: 2a09c66cd3b7c486 4 TUl9fngQi9T2r1Ci1WAnQSqMOPg fig 1a ------- COMMENT: 2a09c66cd3b7c486 7 hdxvZ7Sfk19+heq8/fuGwky/r3k figure 3b ------- COMMENT: 2a09c66cd3b7c486 8 hdxvZ7Sfk19+heq8/fuGwky/r3k figure 3b ------- COMMENT: 2a09c66cd3b7c486 11 fNpmaE7MG19qjD0+PlLVAROJUuU fig 5a ------- COMMENT: 2a09c66cd3b7c486 12 qW0fooJwQWtfFzpmyf80rEliP/A fig 5c ------- COMMENT: 2a09c66cd3b7c486 13 q7yQ4AbC2bEj6KiCpphVWV06cCo dns ------- COMMENT: 2a09c66cd3b7c486 14 yqbm6BUQEhwbitHFmdB2m5PPlNQ (comment: scaffold, platform) ------- COMMENT: 2a09c66cd3b7c486 15 yqbm6BUQEhwbitHFmdB2m5PPlNQ (comment: scaffold, platform) ------- COMMENT: 2a09c66cd3b7c486 16 yqbm6BUQEhwbitHFmdB2m5PPlNQ (comment: scaffold, platform) ------- COMMENT: 2a09c66cd3b7c486 17 yqbm6BUQEhwbitHFmdB2m5PPlNQ (comment: scaffold, platform) ------- COMMENT: 2a09c66cd3b7c486 18 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 2a09c66cd3b7c486 19 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 2a09c66cd3b7c486 20 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 2a09c66cd3b7c486 21 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 2a09c66cd3b7c486 22 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 2a09c66cd3b7c486 23 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 2a09c66cd3b7c486 24 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 2a09c66cd3b7c486 25 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 2a8be2bb0239204b 1 pRANiKBKPjLvMhKt1F3lDg0ECow ------- COMMENT: 2a8be2bb0239204b 2 8CHJjamUtTMf4hG4PaPdELq5LiI Fig. 3A,B ------- COMMENT: 2a8be2bb0239204b 3 nPxJ5j+0U+GKo0U+Z1MBz42y3Wk ------- COMMENT: 2a8be2bb0239204b 4 Fau9ghRrPQ8Q+OIguQNykF2wodw Fig 2b ------- COMMENT: 2a8be2bb0239204b 5 FJrUrY/cNbL7O7xtjP6w5yuBdIM fig 2b ------- COMMENT: 2a8be2bb0239204b 6 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 2a8be2bb0239204b 7 U4DRFYVo1oOmvnzMG6Jr2EfDxQ8 fig 1B, 1D ------- COMMENT: 2a8be2bb0239204b 8 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 2a8be2bb0239204b 9 LkGQQw8FQBPnHuF6zSR3Jquhnkc fig 1C ------- COMMENT: 2a8be2bb0239204b 10 LkGQQw8FQBPnHuF6zSR3Jquhnkc fig 1C ------- COMMENT: 2a8be2bb0239204b 11 GSDoKVHrCsiUO6zYP0Vg8a8VAAQ fig 1D ------- COMMENT: 2a8be2bb0239204b 12 hh64ZrmByb7PfCdakirYxQo7KcE fig 1E ------- COMMENT: 2a8be2bb0239204b 14 OMNA5QZVl21HnwDcnIKd2awbvIc fig 1F ------- COMMENT: 2a8be2bb0239204b 15 nhgAV4vKXywFqIKJ5K22JXrmyTE fig 1 EF ------- COMMENT: 2a8be2bb0239204b 16 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 2a8be2bb0239204b 17 OMNA5QZVl21HnwDcnIKd2awbvIc fig 1F ------- COMMENT: 2a8be2bb0239204b 18 QU9Y0uJyUorENhQQZqil+IGLPM0 fig 1 B ------- COMMENT: 2a8be2bb0239204b 19 dkiXxqTkUijI7aue1tWtH+/webE (Fig. 2B) ------- COMMENT: 2a8be2bb0239204b 20 dkiXxqTkUijI7aue1tWtH+/webE (Fig. 2B) ------- COMMENT: 2a8be2bb0239204b 21 acGVpmK5ESo19l4S0TGlsnyofys Fig S4 ------- COMMENT: 2a8be2bb0239204b 22 eS/sR1mwLsiacd/RPfwhGkASzTw fig S2A ------- COMMENT: 2a8be2bb0239204b 24 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 2a8be2bb0239204b 25 FowQ/bgJRL5ezLbRqNCcVOBFA7k Fig S4 ------- COMMENT: 2a8be2bb0239204b 26 t6uyTtt4P/8kRlIjC2qelSNbzfs Fig 3C ------- COMMENT: 2a8be2bb0239204b 27 t6uyTtt4P/8kRlIjC2qelSNbzfs Fig 3C ------- COMMENT: 2a8be2bb0239204b 29 02Hl7lLP6jsrneT+VjkrPPFqLag (comment: check during interphase) ------- COMMENT: 2a8be2bb0239204b 30 pwcwASlWQtRBBoormzj5gHYObdE fig 4A ------- COMMENT: 2a8be2bb0239204b 31 pwcwASlWQtRBBoormzj5gHYObdE fig 4A ------- COMMENT: 2a8be2bb0239204b 34 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 2a8be2bb0239204b 35 JQsoiA3sA+MpP7qQ01Gr/WxCxS8 Fig. E ------- COMMENT: 2a8be2bb0239204b 37 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 2a8be2bb0239204b 38 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 2a8be2bb0239204b 39 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 2a8be2bb0239204b 40 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 2a8be2bb0239204b 41 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 2a8be2bb0239204b 42 iuydHNwzuGgYLBauiQuie9lIRAU 4B ------- COMMENT: 2a8be2bb0239204b 43 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 2a8be2bb0239204b 44 MRTl98rKQY4u69JsQq5X5gUaN4M fig 5C ------- COMMENT: 2a9bce7ccbdd5c56 2 RoLmRGOosEG5lI7cSNp+i6Yi4Yc Rad21 ------- COMMENT: 2a9bce7ccbdd5c56 4 RoLmRGOosEG5lI7cSNp+i6Yi4Yc Rad21 ------- COMMENT: 2ac0f6929f53ddce 1 R8SsFzPhWYmwe5pD0PCSI3lnijI Figure. 3C, D, E, F and Video 5 ------- COMMENT: 2ac0f6929f53ddce 2 RJMyJFI3FbLbV6pj9mRXWVyTN/I Figure. 5C, D normal (comment: CHECK increased mitochondrial segregation during meiosis) ------- COMMENT: 2ac0f6929f53ddce 3 lbeyPGgzNFKQjikeMyAYvJt8Svg To verify that the attachment to microtubules was not necessary for segregation during meiosis, we employed parental cells lacking the microtubule-mitochondrial linker protein Mmb1 (Fu et al., 2011). Additionally, one of the parental cells had its mitochondria fluorescently labeled. In zygotes and asci resulting from this cross, we observed that parental mito- chondria continued to remain segregated (Fig. S2, C and D). ------- COMMENT: 2ac0f6929f53ddce 4 R8SsFzPhWYmwe5pD0PCSI3lnijI Figure. 3C, D, E, F and Video 5 ------- COMMENT: 2ac0f6929f53ddce 7 ElJiKJ7hIezNAY1xc6i3FCp/+Go "in the absence of Mcp5 in rho+ parental strain (strain PHP4xVA074; see Table S1), only 31.3% of the tetrads dissected (n = 16 tetrads) exhibited mtDNA segregation similar to that observed in Fig. 6 D." ------- COMMENT: 2b2266764f759556 3 NAua1QcTFeaYfG2YntwNVx43wGo It was localized mainly in the cell periphery, probably at the plasma membrane (Fig. 1C), consistent with its role in Pi export. ------- COMMENT: 2b2266764f759556 4 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: 2b2266764f759556 5 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: 2b2266764f759556 6 8jTjtkNnNoFhiY57qk4SkW6skr0 figure 1A ------- COMMENT: 2b2266764f759556 7 8jTjtkNnNoFhiY57qk4SkW6skr0 figure 1A ------- COMMENT: 2b2266764f759556 8 8jTjtkNnNoFhiY57qk4SkW6skr0 figure 1A ------- COMMENT: 2b2266764f759556 9 0u3tNcE+2HglsdOBvbq7MAQedpU We found that spores assumed to be Δpqr1Δxpr1Δvtc4 did not form colonies, suggesting synthetic lethality of gene deletions of pqr1+, xpr1+, and vtc4+ on YES. ------- COMMENT: 2b2266764f759556 10 4nf94FaXk9QqkldM6Fe8/fzs7Sk This time, we obtained Δpqr1Δxpr1Δvtc4 col- onies on PMG with 0.15 mM Pi, which did not grow on either normal PMG (15 mM Pi) or YES. ------- COMMENT: 2b2266764f759556 11 Xfhh7kfH/A+jWPvIUy3jp5jMkEM Cells were severely deformed/swollen and many were collapsed and probably dying (Figs. 2D and S2). ------- COMMENT: 2b2266764f759556 12 /xfkAbnD+5QwVO778a1UOcklXqg All double mutants, Δpqr1Δxpr1, Δpqr1Δvtc4, and Δxpr1Δvtc4, were able to form colonies at 100 mM Pi. Δpqr1Δxpr1 showed slow growth at 100 mM Pi and failed to grow at 300 mM Pi (Fig. 2C). ------- COMMENT: 2b2266764f759556 13 /xfkAbnD+5QwVO778a1UOcklXqg All double mutants, Δpqr1Δxpr1, Δpqr1Δvtc4, and Δxpr1Δvtc4, were able to form colonies at 100 mM Pi. Δpqr1Δxpr1 showed slow growth at 100 mM Pi and failed to grow at 300 mM Pi (Fig. 2C). ------- COMMENT: 2b2266764f759556 14 /xfkAbnD+5QwVO778a1UOcklXqg All double mutants, Δpqr1Δxpr1, Δpqr1Δvtc4, and Δxpr1Δvtc4, were able to form colonies at 100 mM Pi. Δpqr1Δxpr1 showed slow growth at 100 mM Pi and failed to grow at 300 mM Pi (Fig. 2C). ------- COMMENT: 2b2266764f759556 15 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 2b2266764f759556 16 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 2b2266764f759556 17 uYFthibUXAIiy0+4W5G1n7BZ6RM Xpr1-dependent Pi export is accelerated by either Δpqr1 or Δvtc4. Importantly, the elevation of Pi export activity in Δpqr1 and Δvtc4 is synergistic. In Δpqr1Δvtc4, exported Pi was far greater than that in the single mutant (Fig. 5D ------- COMMENT: 2b2266764f759556 18 uYFthibUXAIiy0+4W5G1n7BZ6RM Xpr1-dependent Pi export is accelerated by either Δpqr1 or Δvtc4. Importantly, the elevation of Pi export activity in Δpqr1 and Δvtc4 is synergistic. In Δpqr1Δvtc4, exported Pi was far greater than that in the single mutant (Fig. 5D ------- COMMENT: 2b2266764f759556 19 uYFthibUXAIiy0+4W5G1n7BZ6RM Xpr1-dependent Pi export is accelerated by either Δpqr1 or Δvtc4. Importantly, the elevation of Pi export activity in Δpqr1 and Δvtc4 is synergistic. In Δpqr1Δvtc4, exported Pi was far greater than that in the single mutant (Fig. 5D ------- COMMENT: 2b2266764f759556 20 N+eR4VswJRv7ioSDlbpHKoUr9fM We found that Xpr1-GFP did not increase in Δpqr1, Δvtc4, or Δpqr1Δvtc4 (Fig. 5G). Accelerated Pi export in these mutants may not be caused by increased amounts of Xpr1 protein. ------- COMMENT: 2b2266764f759556 21 N+eR4VswJRv7ioSDlbpHKoUr9fM We found that Xpr1-GFP did not increase in Δpqr1, Δvtc4, or Δpqr1Δvtc4 (Fig. 5G). Accelerated Pi export in these mutants may not be caused by increased amounts of Xpr1 protein. ------- COMMENT: 2b2266764f759556 22 N+eR4VswJRv7ioSDlbpHKoUr9fM We found that Xpr1-GFP did not increase in Δpqr1, Δvtc4, or Δpqr1Δvtc4 (Fig. 5G). Accelerated Pi export in these mutants may not be caused by increased amounts of Xpr1 protein. ------- COMMENT: 2b2266764f759556 23 +xz+imAtPf3KPwRs5G3J7+I2kAc In the mutants, Xpr1- GFP was similarly localized to the cell periphery, suggesting that accelerated Pi export in these mutants may not be caused by dynamic alteration of the localization of the exporter, Xpr1 ------- COMMENT: 2b2266764f759556 24 +xz+imAtPf3KPwRs5G3J7+I2kAc In the mutants, Xpr1- GFP was similarly localized to the cell periphery, suggesting that accelerated Pi export in these mutants may not be caused by dynamic alteration of the localization of the exporter, Xpr1 ------- COMMENT: 2b2266764f759556 25 +xz+imAtPf3KPwRs5G3J7+I2kAc In the mutants, Xpr1- GFP was similarly localized to the cell periphery, suggesting that accelerated Pi export in these mutants may not be caused by dynamic alteration of the localization of the exporter, Xpr1 ------- COMMENT: 2b2266764f759556 27 JTtyBo3cWD5v8ATRcW/zTHS7SAE The Pi hyper-sensitivity of Δpqr1Δxpr1 was suppressed by the double gene deletion of Pho84 and Pho842 (Figs. 6A and S6). ------- COMMENT: 2b478b09a3eb713b 1 XKaCNI1ByeUEuWijWWXw+1TgYtM Of the inhibitors we tested, only iodoacetamide (10 mM) and NEM (10 mM) inhibited Ulp1 activity ------- COMMENT: 2b478b09a3eb713b 2 MQMLW5vPndZbInvkl4QaHEs3TVA NEM, which inhibit the Pmt3-processing activity of Ulp1, and the serine protease inhibitor PMSF, have no effect on Pmt3 deconjugating activity ------- COMMENT: 2b478b09a3eb713b 3 TATzzjxw0++zTSipiayyoyyybWU Fig. 3B ------- COMMENT: 2b478b09a3eb713b 4 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 2b478b09a3eb713b 5 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 2b478b09a3eb713b 6 6YcGa1grLs/+5nYfCv75tYVHHJE Figure 3 C ------- COMMENT: 2b478b09a3eb713b 7 6VWGXWFu58JcyCbhNT2NWdB4JMI (comment: same as rad17 single mutant, epistatic) ------- COMMENT: 2b7ee740ec49d7f4 8 qMu2Wj/twENovlMOs8PCASPpg0Q SpTam41 interacts strongly with cardiolipin ------- COMMENT: 2b8cb5911bb6a83f 3 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 2b8cb5911bb6a83f 4 xLld+U4XtO1Yq5XJ/vA+lPgihjU Fig. 6D ------- COMMENT: 2b8cb5911bb6a83f 5 NEcpAwxSiT8KntgEo8fVXRvMZIg Fig. 6G ------- COMMENT: 2b8cb5911bb6a83f 6 clW0KYjVSszqiWZZf78RB8Z3Z+o Fig. 6H ------- COMMENT: 2b8cb5911bb6a83f 7 tgZJyo2Dx7ENWUDAkbiVuzTFtP4 Fig. 6I ------- COMMENT: 2b8cb5911bb6a83f 8 nq9ZlcNykZjy2U5m9P3xdti4R9s Fig. 6J ------- COMMENT: 2b8cb5911bb6a83f 9 jE2iajuFOR4JEMawZFhqqubAiDY Fig. 6F ------- COMMENT: 2b8cb5911bb6a83f 11 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 2b8cb5911bb6a83f 12 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 2b8cb5911bb6a83f 13 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 2b8cb5911bb6a83f 14 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 2b8cb5911bb6a83f 15 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 2b8cb5911bb6a83f 16 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 2b8cb5911bb6a83f 17 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 2b8cb5911bb6a83f 18 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 2b8cb5911bb6a83f 19 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 2b8cb5911bb6a83f 20 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 21 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 22 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 23 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 24 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 25 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 26 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 27 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 28 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 29 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 30 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 31 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 32 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 33 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 34 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 35 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 36 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 37 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 38 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 39 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 40 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 41 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 42 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 43 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 44 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 45 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 46 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 47 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2b8cb5911bb6a83f 48 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 49 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 50 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 51 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 52 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 53 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 54 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 55 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 56 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 57 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 58 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 59 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 60 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 61 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 62 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 63 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 64 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 65 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 66 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 67 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 68 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 69 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 70 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 71 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b8cb5911bb6a83f 72 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2b8cb5911bb6a83f 73 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2b8cb5911bb6a83f 74 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2b8cb5911bb6a83f 75 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2b8cb5911bb6a83f 76 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2b8cb5911bb6a83f 77 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2b8cb5911bb6a83f 78 jE2iajuFOR4JEMawZFhqqubAiDY Fig. 6F ------- COMMENT: 2b8cb5911bb6a83f 79 NEcpAwxSiT8KntgEo8fVXRvMZIg Fig. 6G ------- COMMENT: 2b8cb5911bb6a83f 80 NEcpAwxSiT8KntgEo8fVXRvMZIg Fig. 6G ------- COMMENT: 2b922d5a01cbc543 3 RHt7AZZDjKNVhxSB/c4nGUYOddk figure 10 (comment: CHECK check specificity). Taken together, the results demonstrate that substitution of Trp-409 in the context of the full-length GIIβ has a moderate to high (60% inhibitory) effect on GII activity. ------- COMMENT: 2b922d5a01cbc543 4 UtY6b1pjLlRlC93wgm7YYdj0bZI figure 10 (comment: HECK check specificity) ------- COMMENT: 2b922d5a01cbc543 5 XcXQ0+EeZF3a5U1xdSMSJQHT1dQ (comment: binds the non trimmed part of the N-glycan) ------- COMMENT: 2b924b1ab23dde19 1 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 2b924b1ab23dde19 2 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 2b924b1ab23dde19 3 F/CibeT0VSFObY6OZ1oV5pg7bqc Fig. 1 Figure 1D shows that the modification is specifically Set9 dependent, as loss of Set9 but not Set1, Set2, Set6, or Clr4 resulted in essentially undetectable mono-, di-, and trimethylated H4-K20. ------- COMMENT: 2b924b1ab23dde19 4 F/CibeT0VSFObY6OZ1oV5pg7bqc Fig. 1 Figure 1D shows that the modification is specifically Set9 dependent, as loss of Set9 but not Set1, Set2, Set6, or Clr4 resulted in essentially undetectable mono-, di-, and trimethylated H4-K20. ------- COMMENT: 2b924b1ab23dde19 5 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 2b924b1ab23dde19 6 QvnR0AHNmd1JgOcGVV6BrPEF7FA Fig. 1 Expression of exogenous wt HA- tagged Set9 but not Set9Y220A was able to rescue H4- K20 methylation in set9 cells (Figure 1E). ------- COMMENT: 2b924b1ab23dde19 7 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 2b924b1ab23dde19 8 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 2b924b1ab23dde19 9 fYsp+Q0CeMKTOZHdW4bp9Nu2D0M Fig. 2B Fission yeast centromeric gene silencing was also found not to be dependent upon H4-K20 methylation (Figures 2B and 2C). ------- COMMENT: 2b924b1ab23dde19 10 Fr4uQVInFDEq0WMzUmGPMeoE1gw resulted in cells hyper- sensitive to DNA damage induced by ultraviolent (UV) light, ionizing radiation (IR), and the topoisomerase I poison camptothecin (CPT) (Figure 3A). ------- COMMENT: 2b924b1ab23dde19 11 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 2b924b1ab23dde19 12 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 2b924b1ab23dde19 13 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 2b924b1ab23dde19 14 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 2b924b1ab23dde19 15 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 2b924b1ab23dde19 16 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 2b924b1ab23dde19 17 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 2b924b1ab23dde19 18 M4Zb5NP44B6Cfb422YWzXcSJOws Genetic studies indicated that loss of Set9 protein further increased the UV sensitivity of excision repair mutants (rad13, uvde, and rad13-uvde, data not shown), arguing that Set9 does not function in excision repair. ------- COMMENT: 2b924b1ab23dde19 19 Ahgo7gCTQINWyylEXfyfRZI97qE set9 cells arrested similar to wt but reentered mitosis markedly faster. Unlike wt, 20%–30% of set9 cells exhibited an abberant or “cut-like” mitotic phenotype. Fig. 4 ------- COMMENT: 2b924b1ab23dde19 20 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 2b924b1ab23dde19 21 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 2b924b1ab23dde19 22 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 2b924b1ab23dde19 23 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 2b924b1ab23dde19 24 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 2b924b1ab23dde19 25 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 2b924b1ab23dde19 26 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 2b924b1ab23dde19 27 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 2b924b1ab23dde19 28 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 2b924b1ab23dde19 29 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 2b924b1ab23dde19 30 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 2b924b1ab23dde19 31 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 2b924b1ab23dde19 32 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 2b924b1ab23dde19 33 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b924b1ab23dde19 34 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 2b924b1ab23dde19 35 4uN2T9tQ2fILJP5xRqxuIq/GPG0 Fig. 5B Clearly, this is not the case, as the sensitivity of the double crb2T215A-set9 mutant is much greater than either single mutant alone and equal to deletion of crb2 (Figure 5B). ------- COMMENT: 2b924b1ab23dde19 36 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2b924b1ab23dde19 37 I9bqmDoKA+GTWb0/SIzCKh6TUHc Fig. 5 Further, the double mutant displays a checkpoint defect equivalent to that of the crb2 mutant (Figure 5C). ------- COMMENT: 2b924b1ab23dde19 38 1Fmj8BagrUge7Q78kBqbKakiRlE Fig. 5 Crb2 phosphorylation is markedly compromised in the absence of Set9, even at low IR doses (Figure 5A). ------- COMMENT: 2b924b1ab23dde19 39 BGGG5MnSC0z8Xj+f6FOtxtPTnnE Fig. 1, 3 and 4 ------- COMMENT: 2b924b1ab23dde19 41 aSH1UoHehaOSoogIzECd67T4pn4 figure 1 ------- COMMENT: 2b924b1ab23dde19 42 BGGG5MnSC0z8Xj+f6FOtxtPTnnE Fig. 1, 3 and 4 ------- COMMENT: 2b924b1ab23dde19 43 BGGG5MnSC0z8Xj+f6FOtxtPTnnE Fig. 1, 3 and 4 ------- COMMENT: 2b924b1ab23dde19 44 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 2b924b1ab23dde19 45 O8dP6fIvB2en/bMVi5dFN+ySBVM Neither loss of Set9 protein, its catalytic activity, nor its H4-K20 substrate rendered cells hypersensitive to TBZ over a range of concentrations (10–30 g/ml) or temperatures (18C–36C) (Figure 2D ------- COMMENT: 2bfae567526bc0ec 1 w+2KtdTflLNIAdvvGREaZTImcjw ------- COMMENT: 2bfae567526bc0ec 2 w+2KtdTflLNIAdvvGREaZTImcjw ------- COMMENT: 2bfae567526bc0ec 3 VKOoAXasn3iCUi2jFgAIH93NCIA (comment: CHECK Abnormal septum phenotype include misplace septum, multi septa and partially formed septa) ------- COMMENT: 2bfae567526bc0ec 4 w+2KtdTflLNIAdvvGREaZTImcjw ------- COMMENT: 2bfae567526bc0ec 5 qqU9i6x/9cAa/QgxNSsdPUiv49M abnormal mitotic arrest with 4C DNA content Cells undergo an extra round of DNA replication without undergoing cytokinesis. ------- COMMENT: 2bfae567526bc0ec 6 +/feedwWpiKSGQ//r9EPqlJuuhg (comment: CHECK child term of abnormal regulation of mitotic metaphase/anaphase transition.) ------- COMMENT: 2bfae567526bc0ec 7 w+2KtdTflLNIAdvvGREaZTImcjw (comment: CHECK pREP5cdc2-DL41 is integrated) ------- COMMENT: 2bfae567526bc0ec 8 /CvHGvUiTi+++N+sAtCZfpdMDh4 About 75% of cells do not enter mitosis in presence of HU Figure 6A, showing that this mutant does not disrupt normal controls regulating entry into mitosis. pRIP45cdc2-DL45 is integrated cdc2-DL41 has same phenotype but it is not clear if it is under the same conditions ------- COMMENT: 2bfae567526bc0ec 9 lCADjBaTlFWL0B8vtPKxdY9vMzc (comment: CHECK pRIP45DL45 is integrated). Figure 6B (comment: CHECK the 20% of cells that are in mitosis are probably cells that were in mitosis when the culture was shifted to the restrictive temperature) ------- COMMENT: 2bfae567526bc0ec 10 7MSYr6COvMXWMm4akzJsCKHKJp8 DNS ------- COMMENT: 2bfae567526bc0ec 11 7MSYr6COvMXWMm4akzJsCKHKJp8 DNS ------- COMMENT: 2bfae567526bc0ec 12 ed5zaEiIKHo3GRU6qoqEbTrFm4Y DNS ------- COMMENT: 2bfae567526bc0ec 13 S/ziUtxe4qe42pxo8YdIOWc36DU DNS ------- COMMENT: 2bfae567526bc0ec 15 wpBEgGfCnZSGOT9gMT/+xwRLSkM ------- COMMENT: 2bfae567526bc0ec 16 fSmB5SnDXNUqkwvvoIplwx+a4v4 ------- COMMENT: 2bfae567526bc0ec 17 rPHuDGQge1pwa14sJF4fjLAW28Q ------- COMMENT: 2bfae567526bc0ec 18 OoH5MyLTHyDHCJ9NJVqgGq5fSuk (comment: CHECK pIRT2suc1 multi copy plasmid partially rescues the pREP5cdc2-DL41 integrant mitotic arrest phenotype and allows formation of micro colonies) (comment: CHECK cdc2-DL45 is also partially rescued) ------- COMMENT: 2bfae567526bc0ec 19 x8Zl/OqvqgGwFVxItXH0y0zJyLQ ------- COMMENT: 2bfae567526bc0ec 21 x8Zl/OqvqgGwFVxItXH0y0zJyLQ ------- COMMENT: 2bfae567526bc0ec 22 x8Zl/OqvqgGwFVxItXH0y0zJyLQ ------- COMMENT: 2bfae567526bc0ec 23 XS2YHn4pNEPhvutS0e2/5n0I5ic (comment: 24% cells enter mitosis compared to 2% when cdc2+ is not over expressed but they did not say that it was a cut phenotype) ------- COMMENT: 2bfae567526bc0ec 24 x8Zl/OqvqgGwFVxItXH0y0zJyLQ ------- COMMENT: 2bfae567526bc0ec 25 k4pFeVB55S0grapW4uQQJ0L8NWI (comment: CHECK cdc2+ is expressed from its own promoter on a multi copy plasmid) ------- COMMENT: 2bfae567526bc0ec 26 R1x4WzDIGcCcLeqpEExkaSzLqIk (comment: CHECK pREP5-DL45 is integrated. cdc2+ is expressed from its own promoter on a multi copy plasmid) ------- COMMENT: 2bfae567526bc0ec 27 D8k2e8dfDqaedA3NJE4MyMhtlMw (comment: CHECK pREP41-DL50 is integrated) ------- COMMENT: 2c0b3f9b052bea9e 1 x1+bRxVRfZuV/nj71lmeubsDd9I (comment: CHECK Figure 1A Zinc-dependent) ------- COMMENT: 2c0b3f9b052bea9e 2 +KX+i/vIAfxcqSEfkbEKIXQE400 (comment: CHECK Figure 6A, dimers form under all growth conditions) ------- COMMENT: 2c0b3f9b052bea9e 3 oXrahwH/EUTq3pU54FPDXMsc++Y fig. 1 ------- COMMENT: 2c0b3f9b052bea9e 4 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 2c0b3f9b052bea9e 5 oXrahwH/EUTq3pU54FPDXMsc++Y fig. 1 ------- COMMENT: 2c0b3f9b052bea9e 6 zR9QopmFnCnuPhXzkwE002TMZfE (comment: The pho8 pho2 double mutant has negligible alkaline phosphatase activity under all growth conditions) ------- COMMENT: 2c0b3f9b052bea9e 7 KPoKGIVWXNMlOrHUSFqJZqa1TjQ ------- COMMENT: 2c0b3f9b052bea9e 8 QsmJy4Z13WjTtTUjxGcQG5Y349I (comment: pho8 transcript and protein levels are increased in high zinc BUT ZINC DEPENDENT CHAnges are independent of transcript levels) ------- COMMENT: 2c0b3f9b052bea9e 9 zH7DPeH3wCSXRszN3hNhuzLZsCE Figure 1B and 1C ------- COMMENT: 2c0b3f9b052bea9e 10 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: 2c0b3f9b052bea9e 11 KntnJkiXmLt+bu4ukaXFz0c82Wk figure 5B ------- COMMENT: 2c0b3f9b052bea9e 12 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: 2c0b3f9b052bea9e 14 +kKTsro4rH7eqszQrfQERuYK6zA reduced during conditions of zinc shock (Figure 8 and Figure 9). (comment: as Pho8 binds its zinc cofactors inside of the secretory pathway, it activity is dependent upon zinc transporters that supply zinc ions to the secretory pathway) ------- COMMENT: 2c0b3f9b052bea9e 15 PAlSCsNJoWMQnn/zm8kYnyn7T7s figure 7 ------- COMMENT: 2c0b3f9b052bea9e 16 R23bB0ygM3OFaEPda5tUzuN2acA reduced alkaline phosphatase activity and Pho8 dimerization (Figures 6C and 6D) ------- COMMENT: 2c0b3f9b052bea9e 17 R23bB0ygM3OFaEPda5tUzuN2acA reduced alkaline phosphatase activity and Pho8 dimerization (Figures 6C and 6D) ------- COMMENT: 2c0b3f9b052bea9e 18 PAlSCsNJoWMQnn/zm8kYnyn7T7s figure 7 ------- COMMENT: 2c0b3f9b052bea9e 19 PAlSCsNJoWMQnn/zm8kYnyn7T7s figure 7 ------- COMMENT: 2c0b3f9b052bea9e 21 aJXCf1oD2z4NLcTcN5wmfjyXUpM consistent with Loz1 facilitating the repression of pho8 gene expression in high zinc (Figure 2A) ------- COMMENT: 2c0b3f9b052bea9e 22 ajk/UIYfM1aHkdd4wOmUq6cnLG4 Pho8 abundance is increased in high zinc in a loz1 deletion strain (Figure 2B and 2C) ------- COMMENT: 2c0b3f9b052bea9e 23 yq4cwx9bP/FyAdo4mhIwW77/RNk figure 4 hat although processing of Pho8 is dependent upon the growth phase of cells, zinc is the major factor that limits Pho8 activity in vivo ------- COMMENT: 2c0b3f9b052bea9e 24 oXrahwH/EUTq3pU54FPDXMsc++Y fig 1 ------- COMMENT: 2c0b3f9b052bea9e 25 tqAaLN7rXlC1v0CSmuKsa2ZxTBQ fig 1 (comment: CHECK abolished?) ------- COMMENT: 2c0b3f9b052bea9e 26 +f6ObC7W7jXnwXnDFruLF+cdHv8 (comment: CHECK abolished ?) ------- COMMENT: 2c0b3f9b052bea9e 27 zH7DPeH3wCSXRszN3hNhuzLZsCE Figure 1B and 1C ------- COMMENT: 2c0b3f9b052bea9e 28 yq4cwx9bP/FyAdo4mhIwW77/RNk figure 4 ....although processing of Pho8 is dependent upon the growth phase of cells, zinc is the major factor that limits Pho8 activity in vivo ------- COMMENT: 2c0b3f9b052bea9e 29 VQwyyMBfQueRCgWSq5NNqBnXO6Q Figure 5D ------- COMMENT: 2c0b3f9b052bea9e 30 VQwyyMBfQueRCgWSq5NNqBnXO6Q Figure 5D ------- COMMENT: 2c0b3f9b052bea9e 31 Ipc8ECFbg2fPZZ2owa/6mq8Xv9E figure 8 ------- COMMENT: 2c0b3f9b052bea9e 33 5WK6C7cjzYbeYjp4Mkv33YuNfzw (comment: CHECK DIRECTLY_ACTIVATES pho8 GO:0004035) As zinc did not affect Pho8 stability, processing, or dimerization, we hypothesized that the activity of Pho8 is directly affected by cellular zinc status. .. .....Taken together these results are consistent with yeast maintaining an inactive 5 Zinc-dependent alkaline phosphatase activity pool of Pho8 in low zinc, which can be rapidly activated as soon as zinc is available. ------- COMMENT: 2c0c6b098c28ca92 1 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 2c0c6b098c28ca92 2 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c0c6b098c28ca92 3 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c0c6b098c28ca92 4 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c0c6b098c28ca92 5 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c0c6b098c28ca92 6 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c0c6b098c28ca92 7 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c0c6b098c28ca92 8 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2c0c6b098c28ca92 9 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2c3138a3a4b94cfc 2 f/SQlBmg2GY227YxkoDTWYbwJoo inferred from silencing and H3-K9 methylation phenotypes ------- COMMENT: 2c3138a3a4b94cfc 3 f/SQlBmg2GY227YxkoDTWYbwJoo inferred from silencing and H3-K9 methylation phenotypes ------- COMMENT: 2c526ab305f015c3 1 SUPbra629VW90IrousqC0OSW07g (comment: Rho1 GTP bound form) (comment: pck2 HR1 domain) ------- COMMENT: 2c526ab305f015c3 5 d6CugVulKcm2RIQF9c9KllN369w ------- COMMENT: 2c526ab305f015c3 7 4yUtpVZaGPPvWQa90GpF48ajJog ------- COMMENT: 2c526ab305f015c3 9 l+f1R919mLn1hkTFl9hvZaQF59A fig 6 ------- COMMENT: 2c526ab305f015c3 10 l+f1R919mLn1hkTFl9hvZaQF59A fig 6 ------- COMMENT: 2c526ab305f015c3 13 Vism6YAO4xBwxkiKJ0qu4NiAwEA (comment: Rho1 appears to have a dual role in stabilizing and localizing Pck proteins) ------- COMMENT: 2c52a27e773dd541 20 /aYlUtHLF57DP+/ilIopFLgE3mc Fig. 1 Intriguingly, we observed that the absence of erd2 caused mitochondrial fragmentation (Fig. 1, A and B) ------- COMMENT: 2c52a27e773dd541 21 pvns5oETRVXg7+anHnTdU1+2+FU Fig. 1C Moreover, the oxygen consumption rate of erd2Δ cells was higher than that of WT cells (Figs. 1C and S1A), suggesting that mitochondrial respi- ration was increased in erd2Δ cells. ------- COMMENT: 2c52a27e773dd541 22 pFedbpVbDQ7R+qNYzd5VwpBbwG8 Fig. 1 As shown in Figure 1, D and E, mitochondrial ROS was higher in erd2Δ cells than in WT cells. ------- COMMENT: 2c52a27e773dd541 23 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 2c52a27e773dd541 24 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 2c52a27e773dd541 25 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 2c52a27e773dd541 26 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 2c52a27e773dd541 27 qwo6huQG37NDaVMJuYwa0+HuXA4 Fig. 2B Under unstressed conditions, that is, when cells were grown on EMM plates, ire1Δ and erd2Δ had no noticeable effect on cell growth, but ire1Δerd2Δ impaired cell growth (Fig. 2B). ------- COMMENT: 2c52a27e773dd541 28 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 2c52a27e773dd541 29 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 2c52a27e773dd541 30 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 2c52a27e773dd541 31 2IUyARLypBFEW37uEv6N6mA8RPs Fig. 3 mitochondria were fragmented in erd2Δ cells but not in WT, ire1Δ, or erd2Δire1Δ cells ------- COMMENT: 2c52a27e773dd541 32 afyyDhie7x5o2dOP/KJOZgMN8gM (comment: CHECK DID WE NEED TO CHANGE THIS TO LOW) Fig. 3 The results showed that the oxygen consumption rate of erd2Δ cells, but not ire1Δ or erd2Δire1Δ cells, was increased. ------- COMMENT: 2c52a27e773dd541 33 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 2c52a27e773dd541 34 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 35 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 36 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 37 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 38 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 39 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 40 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 41 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 42 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 43 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 44 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 45 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 46 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 47 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 48 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 49 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 50 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 51 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 52 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 53 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 54 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 55 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 56 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 57 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 2c52a27e773dd541 58 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2c52a27e773dd541 59 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 2c52a27e773dd541 62 LVkp5VXRKzdFmB0pGDtIFrlcDeo Fig. 5 Cells defective in UPR are sensitive to tunicamycin (32). Consistently, ire1Δ cells failed to grow on EMM plates containing tunicamycin (Fig. 2B) ------- COMMENT: 2c52a27e773dd541 63 4blf/MCJljjHZ8eqfYWxM9Sw+M0 Paradoxically, erd2Δ cells, in which UPR was activated (Fig. 2A), also grew poorly on EMM plates con- taining tunicamycin. ------- COMMENT: 2c59b84cd1c55d1d 1 zct+q1CC+CCDeGvFRygK62HxXIw Fig. 2A (comment: 11 days for visible colonies) ------- COMMENT: 2c59b84cd1c55d1d 3 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2c59b84cd1c55d1d 5 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 2c59b84cd1c55d1d 6 ajYvTe8ClGz9L0syqxdv+jPt8MQ diploid lacked detectable Cm in its tRNAPhe and had normal levels of Gm compared with that from wild type (0.88 versus 0.90 moles/mole) (Table 3; Fig. 4A) ------- COMMENT: 2c59b84cd1c55d1d 7 7vUoSCvhyjWuwVvZlgpw2iOYJbI strain lacked Gm34 in its tRNAPhe, and had Cm levels comparable to those of wild type (0.86 versus 0.91 moles/mole) (Table 3; Fig. 4A). ------- COMMENT: 2c59b84cd1c55d1d 8 NISpC7q2Qf9dThzUsGQV4lkthNk yW formation was impaired in the Sp trm734△ mutant (44% of wild-type levels) and to a lesser extent in the Sp trm732△ mutant (73%) (Fig. 4F), consistent with the increased m1G levels (Table 3; Fig. 4A) ------- COMMENT: 2c59b84cd1c55d1d 9 I8wpJG0OZ8wAAHCtierXuGViEuY ------- COMMENT: 2c59b84cd1c55d1d 11 NISpC7q2Qf9dThzUsGQV4lkthNk yW formation was impaired in the Sp trm734△ mutant (44% of wild-type levels) and to a lesser extent in the Sp trm732△ mutant (73%) (Fig. 4F), consistent with the increased m1G levels (Table 3; Fig. 4A) ------- COMMENT: 2c59b84cd1c55d1d 15 I8wpJG0OZ8wAAHCtierXuGViEuY ------- COMMENT: 2c59b84cd1c55d1d 17 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2c59b84cd1c55d1d 18 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2c59b84cd1c55d1d 19 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2c59b84cd1c55d1d 20 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2c59b84cd1c55d1d 21 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2c59b84cd1c55d1d 22 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2c59b84cd1c55d1d 23 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2c59b84cd1c55d1d 24 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2c59b84cd1c55d1d 25 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2c59b84cd1c55d1d 26 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2c59b84cd1c55d1d 27 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2c59b84cd1c55d1d 28 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2c59b84cd1c55d1d 29 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2c59b84cd1c55d1d 30 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2c59b84cd1c55d1d 31 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2c59b84cd1c55d1d 32 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2c59b84cd1c55d1d 33 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 2c59b84cd1c55d1d 34 ajYvTe8ClGz9L0syqxdv+jPt8MQ diploid lacked detectable Cm in its tRNAPhe and had normal levels of Gm compared with that from wild type (0.88 versus 0.90 moles/mole) (Table 3; Fig. 4A) ------- COMMENT: 2c59b84cd1c55d1d 35 7vUoSCvhyjWuwVvZlgpw2iOYJbI strain lacked Gm34 in its tRNAPhe, and had Cm levels comparable to those of wild type (0.86 versus 0.91 moles/mole) (Table 3; Fig. 4A). ------- COMMENT: 2d3035d3cfa925ea 14 H3GiO9DiltSPLhj2TKe55Pq/daE Figure 3a left panel ------- COMMENT: 2d7a5e210e702a6e 1 Dpp/WaZ6/aGO+fHoEmqTDZbdwa4 A gradient of force is detected along End4 molecule in wildtype cells. ~19 piconewton force near the actin cytoskeleton (G857), ~11 piconewtons near the clathrin lattice (P337), and ~9 piconewtons near the plasma membrane (D155). ------- COMMENT: 2d7a5e210e702a6e 2 T0Is5GqVJ9x4eN2b722tEdFgJBw In ent1-Δ(572-702) cells, force on End4 is increased to above 20 piconewton at G857, but remains the same as in wild type at P337 or D155. ------- COMMENT: 2d7a5e210e702a6e 3 CFutlCTJPaFPHtrPtE3+vrVD+qg In ent1-Δ(572-702) cells, force on End4 is increased to above 20 piconewton at G857, but remains the same as in wild type at P337 and D155. ------- COMMENT: 2d7a5e210e702a6e 4 S/ptuw53wf5Ys4CPg2Mb2MkCTLM In wild-type cells, fluorescently tagged End4p is present at endocytic sites and appears as diffraction-limited puncta (hereafter referred to as End4p patches) that are enriched at cell tips during interphase and around the division plane during mitosis (Fig. 1F; figs. S2A and S5, B and C; and movie S1). ------- COMMENT: 2d7a5e210e702a6e 5 S/ptuw53wf5Ys4CPg2Mb2MkCTLM In wild-type cells, fluorescently tagged End4p is present at endocytic sites and appears as diffraction-limited puncta (hereafter referred to as End4p patches) that are enriched at cell tips during interphase and around the division plane during mitosis (Fig. 1F; figs. S2A and S5, B and C; and movie S1). ------- COMMENT: 2d7a5e210e702a6e 6 S/ptuw53wf5Ys4CPg2Mb2MkCTLM In wild-type cells, fluorescently tagged End4p is present at endocytic sites and appears as diffraction-limited puncta (hereafter referred to as End4p patches) that are enriched at cell tips during interphase and around the division plane during mitosis (Fig. 1F; figs. S2A and S5, B and C; and movie S1). ------- COMMENT: 2d8412a36a34689a 6 hx4/piTgnyM69Y9VgK2+XCUNZhw In hva22Δ cells, reticulophagy was abolished, similar to cells lacking the core autophagy protein Atg1 (Figure 1B). ------- COMMENT: 2d8412a36a34689a 7 ihU21OSmHvRCcvbkZbFrq8bKmaE Next, selective types of autophagy, such as mitophagy and pexophagy, were monitored by the processing of the mitochondrial protein Tuf1- RFP or Sdh2-GFP [21] and the peroxisomal protein Pex11- GFP, respectively. We observed that the hva22Δ mutant was partially defective in both mitophagy (Figure 2D and Fig. S2E) and pexophagy (Figure 2E). ------- COMMENT: 2d8412a36a34689a 8 fKAvUUfb64KmKW4JCZ0rdChHI3I We further found that the inner nuclear membrane protein Lem2 [26] was also degraded in a manner dependent on Hva22 (Figure 1H,I), indicating that the nuclear envelope undergoes Hva22- dependent reticulophagy. (vw I think this should be nucleophagy becasue IMN is not comnnected to ER) ------- COMMENT: 2d8412a36a34689a 9 BCXqBmTukVtPNQRmB+R3u2/2yW0 Consistently, Hva22 was observed on the ER under both nitrogen-rich and starvation conditions (Figure 3C). ------- COMMENT: 2d8412a36a34689a 15 eGiHbOQ7tA+EHc3MV5oAIiaUMl0 The growth defect of the triple mutant strain defective in Spo7, Rtn1, and Yop1 is restored by the overexpression of the budding yeast reticulophagy receptor Atg40, a reticulon- and REEP-like protein that contains an ER-shaping activit ------- COMMENT: 2d8412a36a34689a 16 5kj6zCxPZr5Q2Z+NOoW7EvThzBs (Fig. S4F), the ΔC21–60 and ΔN29 mutants exhibited partial and severe defects in reticulophagy, respectively, whereas the both mutant proteins were detectable on the ER (Fig. S4G) ------- COMMENT: 2d8412a36a34689a 17 h3yAffK/r6CZLDXtVdo3cAVSlko Fig. S4E revealed that the ER-shaping activities of the ΔC21–60 and ΔN29 mutants were partially and severely impaired, respectively (Fig. S4E) ------- COMMENT: 2d8412a36a34689a 18 5kj6zCxPZr5Q2Z+NOoW7EvThzBs (Fig. S4F), the ΔC21–60 and ΔN29 mutants exhibited partial and severe defects in reticulophagy, respectively, whereas the both mutant proteins were detectable on the ER (Fig. S4G) ------- COMMENT: 2d8412a36a34689a 19 h3yAffK/r6CZLDXtVdo3cAVSlko Fig. S4E revealed that the ER-shaping activities of the ΔC21–60 and ΔN29 mutants were partially and severely impaired, respectively (Fig. S4E) ------- COMMENT: 2d9b10ae9f560c52 4 8gBsl3jyBxm6IShaQYHdhk0CLy8 (comment: vw: nda3 tubulin mutant does not assemble spindle and shows Mad2 is localized to unattached kinetochores) ------- COMMENT: 2d9b10ae9f560c52 14 GFnkbeQBaMBe5XZWIWiYM1f0rrQ ...majority of the Mad2-GFP was localized to the spindle ------- COMMENT: 2d9b10ae9f560c52 15 GFnkbeQBaMBe5XZWIWiYM1f0rrQ ...majority of the Mad2-GFP was localized to the spindle ------- COMMENT: 2d9b10ae9f560c52 16 GFnkbeQBaMBe5XZWIWiYM1f0rrQ ...majority of the Mad2-GFP was localized to the spindle ------- COMMENT: 2db803bca64eaafd 40 p/muqccQuG22ixmQ/7lCkQwhPcc same as cdc2-ww single mutant ------- COMMENT: 2dbe85a34294a16f 1 x+RaYNVO+Bu3AP5acUtCQJ1TGK0 These results demonstrate that Yep1 shares the membrane-shaping ability of Rtn1, Yop1, and Tts1 and contributes to the mainte- nance of normal ER structure. ------- COMMENT: 2dbe85a34294a16f 2 x+RaYNVO+Bu3AP5acUtCQJ1TGK0 These results demonstrate that Yep1 shares the membrane-shaping ability of Rtn1, Yop1, and Tts1 and contributes to the mainte- nance of normal ER structure. ------- COMMENT: 2dbe85a34294a16f 8 HKHZBQPVtsuHE/YJcpEEE24GNSE Human REEP1-4 are ER-localizing proteins [38,41,42], Similarly, we found that Yep1 exhibited an ER localization pattern during vegetative growth (S1B Fig). ------- COMMENT: 2dbe85a34294a16f 16 wJ2DLvwIb3BpVndAQ21Ua4gBpnY Using the co-immuno- precipitation assay, we found that Yep1 can self-interact (Fig 3D). ------- COMMENT: 2dbe85a34294a16f 17 +x+EmNOKmhXACGLJU07olrd3lZM As Ost4 and Erg11 localize to both the cortical ER and the nuclear envelope..... (Fig 1F and 1G), ------- COMMENT: 2dbe85a34294a16f 21 +x+EmNOKmhXACGLJU07olrd3lZM As Ost4 and Erg11 localize to both the cortical ER and the nuclear envelope..... (Fig 1F and 1G), ------- COMMENT: 2dbe85a34294a16f 27 jgoGuBgb+Ot76esr6oM9CSFJl3I Fig 1E ------- COMMENT: 2dbe85a34294a16f 28 cLSkOGAq51ZwNiJHFFdI20xY/BI Fig 1C Consistent with this idea, deletion of yep1 severely dimin- ished the relocalization of Ost4-CFP to the vacuole upon DTT treatment (+DTT) or nitrogen starvation treatment (−N) (Fig 1C). ------- COMMENT: 2dbe85a34294a16f 29 QxyA7Kxt4u0SZftovXPYBddoxhU Fig 1D ------- COMMENT: 2dbe85a34294a16f 30 5DvsPEpoKPWZHyiRF005+ytkXFk Fig 1C Consistent with this idea, deletion of yep1 severely dimin- ished the relocalization of Ost4-CFP to the vacuole upon DTT treatment (+DTT) or nitrogen starvation treatment (−N) (Fig 1C). yep1Δ cells also exhibited a severe defect in the autophagic processing of GFP-tagged integral ER membrane protein Erg11 into free GFP (Fig 1D and 1E). ------- COMMENT: 2dbe85a34294a16f 31 we6uzYtFOHGSiSeFOcw/IfrT5m8 Fig 1J. In contrast to the severe ER-phagy and nucleophagy defects of yep1Δ cells, bulk autophagy in yep1Δ cells was largely normal as indicated by the processing of CFP-Atg8 (Fig 1J). In addi- tion, another readout of bulk autophagy, the processing of fluorescent protein-tagged cytosolic protein Tdh1 (glyceraldehyde-3-phosphate dehydrogenase (GAPDH)) [43], was also largely unaffected in yep1Δ cells (S1E Fig). Consistent with the lack of bulk autophagy defects, trans- mission (TEM) analysis showed that autophagosome accumulation in the fsc1Δ mutant, which is defective in autophagosome–vacuole fusion [44], was not notably affected by the deletion of yep1 (S1F Fig). ------- COMMENT: 2dbe85a34294a16f 32 AFCUEU9apxzOi6Wa06cMH11yYYs Fig 1J. In contrast to the severe ER-phagy and nucleophagy defects of yep1Δ cells, bulk autophagy in yep1Δ cells was largely normal as indicated by the processing of CFP-Atg8 (Fig 1J). ------- COMMENT: 2dbe85a34294a16f 33 To/EniUTbsuUK7u4PQHEyIzBWkU Fig. 1I. Deletion of yep1 abolished the processing of Pus1-mECitrine in both DTT- and starvation-treated cells, indicating that Yep1 is essential for nucleophagy. ------- COMMENT: 2dbe85a34294a16f 34 HnYiak1OUkNlzQLoqjbPfVCFmxg Fig 1H ------- COMMENT: 2dbe85a34294a16f 37 t6uyTtt4P/8kRlIjC2qelSNbzfs Fig 3C ------- COMMENT: 2dbe85a34294a16f 38 1eCqN4/GWYFaYsqtoNSGYvo7Geo Fig 3C We observed that yep1Δ caused a noticeable but even weaker septum positioning defect than rtn1Δ, yop1Δ, or tts1Δ. Combined yep1Δ with the double and triple deletion of rtn1, yop1, and tts1 invariably resulted in a more severe phenotype (Fig 3C). ------- COMMENT: 2dbe85a34294a16f 41 +x+EmNOKmhXACGLJU07olrd3lZM As Ost4 and Erg11 localize to both the cortical ER and the nuclear envelope..... (Fig 1F and 1G), ------- COMMENT: 2dbe85a34294a16f 42 +x+EmNOKmhXACGLJU07olrd3lZM As Ost4 and Erg11 localize to both the cortical ER and the nuclear envelope..... (Fig 1F and 1G), ------- COMMENT: 2dbe85a34294a16f 43 KarKaq81MywdJsLeSavt1yTH0P0 The loss of the ER-phagy receptor Epr1 diminished the processing of Pus1-mECi- trine, suggesting that Epr1 also acts as a nucleophagy receptor ------- COMMENT: 2dbe85a34294a16f 44 jwTNfYSGqVqv9QUsNN8RESGkhAo In both wild-type and yep1Δ cells, Epr1 and Atg8 colocalized at punctate structures shortly after ER-phagy induction by DTT or starvation treatment (S2A Fig), indicating that Yep1 is not essential for the initial stage of ER-phagy during which Epr1 mediates a connection between the ER and Atg8-decorated autophagic membranes. ------- COMMENT: 2dbe85a34294a16f 45 kOjpxz92ghwfaiqhYvNDen9X0lE (comment: during nucleophagy abnd reticulophagy) ------- COMMENT: 2dbe85a34294a16f 46 Tjf3Mcyz6m10g0f7creU3aNoWTY Figure 2. Taken together, the above findings demonstrate that Yep1 is required for the autophagoso- mal enclosure of ER-phagy/nucleophagy cargos. ------- COMMENT: 2dbe85a34294a16f 47 Tjf3Mcyz6m10g0f7creU3aNoWTY Figure 2. Taken together, the above findings demonstrate that Yep1 is required for the autophagoso- mal enclosure of ER-phagy/nucleophagy cargos. ------- COMMENT: 2dbe85a34294a16f 48 PfphRPoqKp6U8WdHtXfXaCnvEiQ In the absence of Rtn1, Yop1, and Tts1, the cortical ER becomes less reticulate and more sheet-like, with the frequent appearance of large holes in images of the top or bottom plane of the cells and extended gaps in images of the midplane of the cells [50]. We found that this alter- ation of ER morphology can be reversed to a large extent by either introducing back Rtn1 or increasing the expression level of Yep1 (Figs 3B and S4B). ------- COMMENT: 2dbe85a34294a16f 49 Tjf3Mcyz6m10g0f7creU3aNoWTY Figure 2. Taken together, the above findings demonstrate that Yep1 is required for the autophagoso- mal enclosure of ER-phagy/nucleophagy cargos. ------- COMMENT: 2dbe85a34294a16f 50 F2hdc70oYO3U+yWTohIcaiGvgKM Thus, Yep1, when overexpressed, can fulfill the function of maintaining tubular ER independently of Rtn1, Yop1, and Tts1. ------- COMMENT: 2dbe85a34294a16f 51 dNfIcAGnZ3jjnnE8drwaI1im960 In the absence of Rtn1, Yop1, and Tts1, the cortical ER becomes less reticulate and more sheet-like, with the frequent appearance of large holes in images of the top or bottom plane of the cells and extended gaps in images of the midplane of the cells [50]. We found that this alter- ation of ER morphology can be reversed to a large extent by either introducing back Rtn1 or increasing the expression level of Yep1 (Figs 3B and S4B) ------- COMMENT: 2dbe85a34294a16f 52 W82RoxiYS9HlgNhWj39daaWEhzw Fig3 L ------- COMMENT: 2dbe85a34294a16f 53 ddZi6eIl0M+RoeG7Gs8QDcAlfKQ Fig3 J-K；Fig S6 E ------- COMMENT: 2dbe85a34294a16f 54 ddZi6eIl0M+RoeG7Gs8QDcAlfKQ Fig3 J-K；Fig S6 E ------- COMMENT: 2dbe85a34294a16f 55 LaLrlW//4gaNpJCQ7ChqmadCSUQ Fig S6 A ------- COMMENT: 2dbe85a34294a16f 56 ddZi6eIl0M+RoeG7Gs8QDcAlfKQ Fig3 J-K；Fig S6 E ------- COMMENT: 2dbe85a34294a16f 57 W82RoxiYS9HlgNhWj39daaWEhzw Fig3 L ------- COMMENT: 2dbe85a34294a16f 58 W82RoxiYS9HlgNhWj39daaWEhzw Fig3 L ------- COMMENT: 2dbe85a34294a16f 59 +sKz8ttMtoPnOsFnV3sQ8xthVB4 Fig3 K ------- COMMENT: 2dbe85a34294a16f 60 +sKz8ttMtoPnOsFnV3sQ8xthVB4 Fig3 K ------- COMMENT: 2dbe85a34294a16f 62 BccHJrhlRuTLt+eE7VtS4gNDx0Q Fig S5； Fig S6 ------- COMMENT: 2dbe85a34294a16f 63 BccHJrhlRuTLt+eE7VtS4gNDx0Q Fig S5； Fig S6 ------- COMMENT: 2dbe85a34294a16f 64 BccHJrhlRuTLt+eE7VtS4gNDx0Q Fig S5； Fig S6 ------- COMMENT: 2dbe85a34294a16f 65 XYSQbp2BOGIATX0HnehQEt6LsT0 Fig S5; Fig S6 ------- COMMENT: 2dbe85a34294a16f 66 Fbz1U7bCxi+V9lXdzb35kqQOhOk Fig S6A ------- COMMENT: 2e22c541f5e9e87c 2 4CFpbvxiFiJsBViZ1iYyzDRWGuk (comment: CHECK also fzr2 &3) ------- COMMENT: 2e22c541f5e9e87c 11 kryjU6Lh0RjkoLOumnOHVHtMXcQ ------- COMMENT: 2e22c541f5e9e87c 21 8kVu/9gMy34xNeRqsY8LJczGk4g (comment: I'm not completely sure if the Slp1-APC degrades mes1 or only ubiquitinates it, but this is most likely correct?...) ------- COMMENT: 2e22c541f5e9e87c 48 8ef8/ZZlwmfH3yCq4lLTah35zV4 fig 1B: 4 nuclei appear later than normal. ------- COMMENT: 2e22c541f5e9e87c 49 8ef8/ZZlwmfH3yCq4lLTah35zV4 fig 1B: 4 nuclei appear later than normal. ------- COMMENT: 2e22c541f5e9e87c 50 8ef8/ZZlwmfH3yCq4lLTah35zV4 fig 1B: 4 nuclei appear later than normal. ------- COMMENT: 2e22c541f5e9e87c 53 BaEobgIbq4RpWhft4asbzUA9FY4 fig 1c, no tetranucleates ------- COMMENT: 2e22c541f5e9e87c 55 3yqq8mT6r2p3L3RL/sFe2Tak0os fig 2A ------- COMMENT: 2e22c541f5e9e87c 56 3yqq8mT6r2p3L3RL/sFe2Tak0os fig 2A ------- COMMENT: 2e22c541f5e9e87c 57 Krt8IEN2thpV+MLPwLEVMzonARU fig 2B ------- COMMENT: 2e22c541f5e9e87c 58 Krt8IEN2thpV+MLPwLEVMzonARU fig 2B ------- COMMENT: 2e22c541f5e9e87c 68 yzQNqL/xG7vyo/srTR/Y5QAfEtU fig 6C ------- COMMENT: 2e22c541f5e9e87c 69 yzQNqL/xG7vyo/srTR/Y5QAfEtU fig 6C ------- COMMENT: 2e22c541f5e9e87c 76 u4h9s5wkcnm3kQoaW9fY2oQ7YqM fig 7A ------- COMMENT: 2e22c541f5e9e87c 77 u4h9s5wkcnm3kQoaW9fY2oQ7YqM fig 7A ------- COMMENT: 2e22c541f5e9e87c 78 u4h9s5wkcnm3kQoaW9fY2oQ7YqM fig 7A ------- COMMENT: 2e22c541f5e9e87c 79 u4h9s5wkcnm3kQoaW9fY2oQ7YqM fig 7A ------- COMMENT: 2e22c541f5e9e87c 80 LdMWFsO2r6UZuf0Jza9r0WbGDzU (comment: also fzr3) ------- COMMENT: 2e22c541f5e9e87c 82 3yqq8mT6r2p3L3RL/sFe2Tak0os fig2A ------- COMMENT: 2e22c541f5e9e87c 83 Krt8IEN2thpV+MLPwLEVMzonARU fig2B ------- COMMENT: 2e5593fd20311c48 23 k9Adcdh5hDWv9nM0GVOml1eaIlE (comment: E. coli ispA mutant used as assay system) ------- COMMENT: 2e5593fd20311c48 30 k9Adcdh5hDWv9nM0GVOml1eaIlE (comment: E. coli ispA mutant used as assay system) ------- COMMENT: 2e5593fd20311c48 31 k9Adcdh5hDWv9nM0GVOml1eaIlE (comment: E. coli ispA mutant used as assay system) ------- COMMENT: 2e7e74bebcf07eb3 57 I/XnB3San7YGtJHTrWqEHJ06sCw (Figure 3b) ------- COMMENT: 2e7e74bebcf07eb3 59 I/XnB3San7YGtJHTrWqEHJ06sCw (Figure 3b) ------- COMMENT: 2e7e74bebcf07eb3 60 I/XnB3San7YGtJHTrWqEHJ06sCw (Figure 3b) ------- COMMENT: 2ef9911b1699da5e 33 Lpb/TWDBygfAaHqT/9CKPRl2B98 (comment: nuclease-dead allele) ------- COMMENT: 2ef9911b1699da5e 60 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 2ef9911b1699da5e 61 wgQVuyuEMLLI6mf8hAWnGN++Xvk no expressivity extension because of decreased growth when untreated ------- COMMENT: 2ef9911b1699da5e 62 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 2ef9911b1699da5e 65 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 2ef9911b1699da5e 66 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 2ef9911b1699da5e 67 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 2ef9911b1699da5e 94 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 2ef9911b1699da5e 95 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 2ef9911b1699da5e 96 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 2f1c2ad9160b5b20 5 MdeI3c/UUN5bWXP6dg/yb6mqfrc (comment: CHECK removed during glucose starvation) (comment: CHECK observed during nitrogen starvation) (comment: CHECK S546) ------- COMMENT: 2f1c2ad9160b5b20 7 cRf1Ps9RORIW4RJlPkCQ6qw94T0 (comment: CHECK removed during nitrogen starvation) (comment: T415) ------- COMMENT: 2f1c2ad9160b5b20 9 ZdgifvxcssgBj7E1YRSI8MXhoW4 (comment: in the presence of glucose) ------- COMMENT: 2f1c2ad9160b5b20 11 9BNgqe9n5vB+zTanhUWsWMFpMDs (comment: observed in the presence/absence of glucose) ------- COMMENT: 2f1c2ad9160b5b20 12 9BNgqe9n5vB+zTanhUWsWMFpMDs (comment: observed in the presence/absence of glucose) ------- COMMENT: 2f1c2ad9160b5b20 13 9BNgqe9n5vB+zTanhUWsWMFpMDs (comment: observed in the presence/absence of glucose) ------- COMMENT: 2f1c2ad9160b5b20 19 ZuroSy/C4wKTng6grwy7U5IhUo0 (comment: decreased during glucose starvation) ------- COMMENT: 2f1c2ad9160b5b20 21 EpE66EYJLV3W/ExH9F4XxKjAIxY (comment: CHECK strong phenotype = has_severity(FYPO_EXT:0000001)) ------- COMMENT: 2f34923cbe339952 1 0UxORu9Aw8xDnTiK797rLNs4eeU Fig. 1E and F ------- COMMENT: 2f34923cbe339952 2 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 2f34923cbe339952 3 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 2f34923cbe339952 4 8THrXOcssh2r2DutBUTLchxvGlU We found that a rad21-45 mutant was unable to arrest in the presence of Lat B. Fig. 2B and C ------- COMMENT: 2f34923cbe339952 5 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 2f34923cbe339952 6 x/e1S+GWovT6wQh7N0GgKd1howI Cells that lack Mad2 arrest in metaphase in the presence of Lat B (Fig. 3a) ------- COMMENT: 2f34923cbe339952 7 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 2f34923cbe339952 8 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 2f34923cbe339952 9 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 2f34923cbe339952 10 vkT8uMpiBxgGlmYXEA8V3+LXigM Fig. 4E ------- COMMENT: 2f34923cbe339952 11 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 2f34923cbe339952 12 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 2f34923cbe339952 13 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 2f34923cbe339952 14 kETDvlfmAhpG7lOFikluLMj2yOU Importantly, we found that synchronized Datf1 cells completely failed to arrest nuclear division in the presence of Lat B (Fig. 4d) ------- COMMENT: 2f34923cbe339952 15 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 2f34923cbe339952 16 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 2f34923cbe339952 17 9Xb+uz4ftlqagKGp7TY1gJc7WdA Fig. 4F ------- COMMENT: 2f3eb8594559cc00 1 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 2 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 3 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 4 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 5 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 6 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 7 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 8 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 9 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 10 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 11 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 12 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 13 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 14 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 15 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 16 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 17 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 18 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 19 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 20 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 21 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 22 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 23 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 24 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 25 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 26 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 27 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 28 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 29 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 30 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 31 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 32 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 33 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 34 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 35 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 36 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 37 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 38 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 39 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 40 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 41 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 42 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 2f3eb8594559cc00 43 LQmuKZbL9ND56pzzWctDT58tWRE Figure 3C ------- COMMENT: 2f3eb8594559cc00 44 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 2f3eb8594559cc00 45 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 2f3eb8594559cc00 46 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 2f3eb8594559cc00 47 MtNsApt5HI6ljJgoPqRkv5JuJK8 Figure 5D ang G ------- COMMENT: 2f3eb8594559cc00 48 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: 2f3eb8594559cc00 49 /r8uIw/Hl4lLtIoIHMsNKjkXX0E Figure 6, 7, 9 and 10 ------- COMMENT: 2f3eb8594559cc00 50 riH6PuZeW9pd2w953irT9kPPwpE Figure 8 ------- COMMENT: 2f3eb8594559cc00 51 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 2f3eb8594559cc00 52 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 2f3eb8594559cc00 53 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 2f3eb8594559cc00 54 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 55 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 56 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 57 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 58 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 59 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 60 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 61 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 62 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 63 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 64 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 65 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 66 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 67 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 68 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 69 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 70 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 71 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 72 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 73 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 74 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 75 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 76 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 77 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 78 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 79 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 2f3eb8594559cc00 80 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 2f3eb8594559cc00 81 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 2f3eb8594559cc00 82 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 2f3eb8594559cc00 83 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 2f54522ed2a9d3d2 1 HhOz8a4A16CTWPvyoylxF2Z55s0 (comment: CHECK present in cycling cells and meiosis I cells. Required for cellular resistance to MMS and CPT.) ------- COMMENT: 2f54522ed2a9d3d2 2 oS7h/bI0PlQaO2l4czyrvR7eeH8 (comment: CHECK Enhanced interaction upon MMS treatment) ------- COMMENT: 2f54522ed2a9d3d2 3 Iemd06acZFfypwjt65xGZRfj0+c (comment: CHECK Required for cellular resistance to MMS and CPT) ------- COMMENT: 2f54522ed2a9d3d2 9 rq+0vfCNTlwZlQEXjjvA/Q4UCPo (comment: CHECK Epistatic genetic interaction, same as mus81delta alone) ------- COMMENT: 2f54522ed2a9d3d2 16 W2IPEbF1/Q7VZdzxiisvnQseuLc (comment: CHECK Epistatic genetic interaction, same as eme1delta alone) ------- COMMENT: 2f54522ed2a9d3d2 17 rq+0vfCNTlwZlQEXjjvA/Q4UCPo (comment: CHECK Epistatic genetic interaction, same as mus81delta alone) ------- COMMENT: 2f54522ed2a9d3d2 21 rq+0vfCNTlwZlQEXjjvA/Q4UCPo (comment: CHECK Epistatic genetic interaction,same as mus81delta alone) ------- COMMENT: 2f54522ed2a9d3d2 22 W2IPEbF1/Q7VZdzxiisvnQseuLc (comment: CHECK Epistatic genetic interaction, same as eme1delta alone) ------- COMMENT: 2f54522ed2a9d3d2 34 WHlyYt6ljKIc1Afog4N8tW6nVDc (comment: CHECK slightly worse than srs2delta alone) ------- COMMENT: 2f54522ed2a9d3d2 45 rq+0vfCNTlwZlQEXjjvA/Q4UCPo (comment: CHECK Epistatic genetic interaction, same as mus81delta alone) ------- COMMENT: 2f54522ed2a9d3d2 47 WHlyYt6ljKIc1Afog4N8tW6nVDc (comment: CHECK slightly worse than srs2delta alone) ------- COMMENT: 2f54522ed2a9d3d2 49 HhOz8a4A16CTWPvyoylxF2Z55s0 (comment: CHECK present in cycling cells and meiosis I cells. Required for cellular resistance to MMS and CPT.) ------- COMMENT: 2f5adb6aec169e1f 10 ibmPId0LZ0GKptc12eNZYyjaZpE ((comment: CHECK increased localization of mad2 to kinetochore) ------- COMMENT: 2f93fe0b9007bce3 1 jZPgWNXV5gtfapCq9hHH/f3//zQ We conclude that pik1-11 cells lack a Golgi PI4P pool, and have reduced PM PI4P that does not result in a corresponding decrease in PM PI(4,5)P2. ------- COMMENT: 2f93fe0b9007bce3 2 rHBHdArmTcSUKj047S8llrD3HPw ------- COMMENT: 2f93fe0b9007bce3 3 s68Xb/+5ESQIZ6fzIJdmwv9BoTI found co-localization only with the trans-Golgi marker (Fig. 3B), ------- COMMENT: 2f93fe0b9007bce3 4 w5h4fepQVukCh2+VOUSRbOZ5Pdg we observed co-localization of Ncs1-mCherry with Pik1- D450-mNG at the trans-Golgi (Fig. 4B) ------- COMMENT: 2f93fe0b9007bce3 5 4JJkgqWqIiB4LFcCQQJD33rkluo We conclude that pik1-11 cells lack a Golgi PI4P pool, and have reduced PM PI4P that does not result in a corresponding decrease in PM PI(4,5)P2. ------- COMMENT: 2f93fe0b9007bce3 6 RPQbTvPmB+rEUYShPQpw/awvJrc (Fig. 1D-E). ------- COMMENT: 2f93fe0b9007bce3 7 nUCcEDoowWdBc8w5z3A4wbzAAHc Figure 1F ------- COMMENT: 2f93fe0b9007bce3 10 5rWE6ii3zH6OgNNz87kbsvzgEbI ------- COMMENT: 2f93fe0b9007bce3 11 5rWE6ii3zH6OgNNz87kbsvzgEbI ------- COMMENT: 2f93fe0b9007bce3 12 5rWE6ii3zH6OgNNz87kbsvzgEbI ------- COMMENT: 2f93fe0b9007bce3 13 gpB4+oBmDN70CFWdI7ffXvkmhLg In contrast, PM Its3-mNG was mildly reduced at 25°C and increased 1.8-fold at 36°C in pik1-11 compared to wildtype cells (Fig. 2E-F and S1E) ------- COMMENT: 2f93fe0b9007bce3 15 s19eY5HAoGQcqxGUId9SqLEmG20 when we attempted to combine ncs1Δ with pik1-11 we found that they were synthetically lethal (Fig. 4A) ------- COMMENT: 2f93fe0b9007bce3 16 2ljUbn2Isb0VY8y2K/mmpG3+gTM Fig. S2A ------- COMMENT: 2f93fe0b9007bce3 17 2ljUbn2Isb0VY8y2K/mmpG3+gTM Fig. S2A ------- COMMENT: 2f93fe0b9007bce3 18 cvkMdM8QS1L+MgNVPYyZTcKo6PY We found that Pik1-D450- mNG still localized to the trans-Golgi marked by Sec72-mCherry in ncs1∆ cells (Fig. 4C), Interestingly, there was a high cytoplasmic Pik1 population in ncs1Δ cells that was not observed in wildtype cells; indeed, there was >2- fold more Pik1 overall (Fig. 4D). We currently do not have a mechanistic explanation for this observation. ------- COMMENT: 2f93fe0b9007bce3 19 5rWE6ii3zH6OgNNz87kbsvzgEbI ------- COMMENT: 2f93fe0b9007bce3 20 TxppIdImpG9WlJxmqz5xeXFoBRA ------- COMMENT: 2f93fe0b9007bce3 22 d2NdxnatvYWNnh9afhcOiSOs0XA (Fig. S1D) ------- COMMENT: 2f93fe0b9007bce3 23 d2NdxnatvYWNnh9afhcOiSOs0XA (Fig. S1D) ------- COMMENT: 2f93fe0b9007bce3 24 d2NdxnatvYWNnh9afhcOiSOs0XA (Fig. S1D) ------- COMMENT: 2f93fe0b9007bce3 25 p6rOWB8Z9MVWfGLR3QJmP/vrEsM pik1-11 cells grow similarly to wildtype at 25 ̊C and 29 ̊C but pik1-11 cells do not grow at 32 ̊C or 36 ̊C (Fig. 1C) ------- COMMENT: 2f93fe0b9007bce3 26 p6rOWB8Z9MVWfGLR3QJmP/vrEsM pik1-11 cells grow similarly to wildtype at 25 ̊C and 29 ̊C but pik1-11 cells do not grow at 32 ̊C or 36 ̊C (Fig. 1C) ------- COMMENT: 2f93fe0b9007bce3 31 ANpxaepHbX/7tbY1Hivqf1b5XYY To determine whether there was Golgi PI4P in ncs1∆, a proxy for a change in Pik1 activity, we imaged cells expressing GFP-P4CSidC in wildtype and ncs1Δ cells. We observed the persistence of Golgi PI4P puncta as well as increased cytoplasmic and PM PI4P levels in ncs1∆ cells (Fig. 4E-F). Combined with the fact that Pik1 is essential whereas Ncs1 is not (Hamasaki-Katagiri et al., 2004; Park et al., 2009), it seems unlikely that Ncs1 is required for S. pombe Pik1 activity and perhaps even acts as a negative regulator. ------- COMMENT: 2f93fe0b9007bce3 32 8kSA4LM32UoWIhW/G2GBMFrKkCU This is known from the MF but can be futher supported from the fact that PIP4 levels decrease when stt4 is not localized correctly ------- COMMENT: 2fe0a48657ba4f41 1 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 2fe0a48657ba4f41 2 61F+OMpAhQCvEaz7sMKB21qNKf0 (Fig. 1B and E) ------- COMMENT: 2fe0a48657ba4f41 3 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 2fe0a48657ba4f41 4 RNueUADQksa2OrTruAjsH6RdNBo (Fig. 1D, E and F) ------- COMMENT: 2fe0a48657ba4f41 5 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 6 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 7 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 8 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 9 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 10 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 11 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 12 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 13 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 14 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 15 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 16 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 17 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 18 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 19 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 20 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 21 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 22 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 23 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 24 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 25 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 26 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 27 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 28 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 29 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 30 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 31 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fe0a48657ba4f41 32 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 2fed9a1e695623df 20 EGkP0UH11LteHSWDAlcpeAHd2w8 (comment: CHECK is response to heat/response to denatured protein) ------- COMMENT: 2ff9544218a3c825 4 Gdbv8t3f1IIO8zipv8nnV7uVgFY (comment: CHECK affecting mei2) ------- COMMENT: 2ff9544218a3c825 10 oQYBO8Am2EQfNw66nq9hR49NRGE (comment: CHECK affecting Gad8 S546 phosphorylation) ------- COMMENT: 2ff9544218a3c825 11 KKJ3Pj4b7eOPq0CrXvSOENJXey0 Overexpression of tor2 is rescued by deletion of pab1 in mating and mei2 RNA expression under nitrogen starvation conditions ------- COMMENT: 2ff9544218a3c825 12 MCsYW15jhY3rtaPnmzlYpsYBi3g (comment: CHECK mei2 expression upon nitrogen starvation) ------- COMMENT: 2ff9544218a3c825 13 lEu8A+UNr44oEovNu9FQuDf6I7U (comment: CHECK hyperfertility and mei2 RNA expression upon nitrogen starvation) ------- COMMENT: 2ff9544218a3c825 14 AhEUJZOdR9vq+Us5wwRZkGTPKt8 (comment: CHECK has substrate gad8) ------- COMMENT: 2ff9544218a3c825 15 sobZd08PMT5yd8JWD2TYixMtHT4 (comment: CHECK S546 added by tor1, added during cellular response to nitrogen starvation) ------- COMMENT: 2ffb56fc6f8cf07d 3 l5vUksyWlTRRVn+r7TsmAX38DuA Zhf1 is required for the rapid transport of zinc ions out of the cytosol during a zinc shock (a condition where there is a rapid influx of zinc into a cell) ------- COMMENT: 2ffb56fc6f8cf07d 4 ukaEH2+UwvLNbZTPIZWGYLd2FRE A key finding of our work is that Zhf1 does NOT transport zinc out of the cytosol under conditions of zinc ion starvation ------- COMMENT: 2ffb56fc6f8cf07d 5 vpUsiVku2upAjxvgsCBo+sJ08nI Fig 5A/B In this paper we used a high affinity zinc-responsive FRET sensor (ZapCY1) to measure zinc ion availability in the cytosol under conditions of zinc deficiency. Thus, in addition to accumulating high levels of total cellular zinc - this manuscript shows that loz1D cells also accumulate higher levels of zinc in the cytosol. This accumulation is also dependent upon Zrt1 as this phenotype is not observed in a loz1 zrt1 double mutant ------- COMMENT: 2ffb56fc6f8cf07d 6 qOB0Z2S5GW/TSt20VCT+TT52xvk The reporter gene was the the ZapCY1 high affinity zinc-responsive FRET reporter. As this reporter is located in the cytosol and nucleus it measures zinc availability in these compartments (so the Term name should really be increased cytoplasm (not cellular) zinc level) ------- COMMENT: 2ffb56fc6f8cf07d 7 6Xjvq+OMH0yvFwGVcQfQerqmzOc The experiment performed was to measure total cellular zinc ion levels in a zrt1D strain during a zinc shock (Figure 4A) ------- COMMENT: 2ffb56fc6f8cf07d 8 Pn7n7RZPvQrGW9YmJR3xKmK5m4o We made the term "zinc ion import into organelle"in GO becuse it fits better witht the descendants ------- COMMENT: 2ffb56fc6f8cf07d 9 tBCSoBjTmV8woOF9ogVPQXOedQk The reporter gene was the the ZapCY1 high affinity zinc-responsive FRET reporter. As this reporter is located in the cytosol and nucleus it measures zinc availability in these compartments (so the Term name should really be increased cytoplasm (not cellular) zinc level) ------- COMMENT: 2ffb56fc6f8cf07d 11 qOB0Z2S5GW/TSt20VCT+TT52xvk The reporter gene was the the ZapCY1 high affinity zinc-responsive FRET reporter. As this reporter is located in the cytosol and nucleus it measures zinc availability in these compartments (so the Term name should really be increased cytoplasm (not cellular) zinc level) ------- COMMENT: 2ffb56fc6f8cf07d 12 qOB0Z2S5GW/TSt20VCT+TT52xvk The reporter gene was the the ZapCY1 high affinity zinc-responsive FRET reporter. As this reporter is located in the cytosol and nucleus it measures zinc availability in these compartments (so the Term name should really be increased cytoplasm (not cellular) zinc level) ------- COMMENT: 2ffb56fc6f8cf07d 13 lCNpThCWDHh3Wl4z7y5rAbK59tI The reporter genes used were the ZapCY1 high affinity and ZapCY2 low affinity zinc-responsive FRET reporter. As this reporter is located in the cytosol and nucleus it measures zinc availability in these compartments (so the Term name should really be increased cytoplasm (not cellular) zinc level) ------- COMMENT: 2ffb56fc6f8cf07d 15 UwI9QEkjoF4ikVeAEN47/7MNa5U Zrg17 also transports zinc out of the cytosol when zinc is available (as well as when it is limiting) ------- COMMENT: 2ffb56fc6f8cf07d 16 KCPhRCsFCRs+9pfZPHlypTgmkGM Fig 4 BE ------- COMMENT: 2ffb56fc6f8cf07d 17 bi5Tf2gkuaSK9nPeor76pOXduP0 Figure 1 ((comment: CHECK EDTA, zinc chelator) ------- COMMENT: 2ffb56fc6f8cf07d 18 bi5Tf2gkuaSK9nPeor76pOXduP0 Figure 1 ((comment: CHECK EDTA, zinc chelator) ------- COMMENT: 2ffb56fc6f8cf07d 21 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: 2ffb56fc6f8cf07d 24 steeyeTIf0tHuMWCSd7Sj4GAFSY Fig 8 ------- COMMENT: 2ffb56fc6f8cf07d 25 steeyeTIf0tHuMWCSd7Sj4GAFSY Fig 8 ------- COMMENT: 2ffb56fc6f8cf07d 26 steeyeTIf0tHuMWCSd7Sj4GAFSY Fig 8 ------- COMMENT: 2ffb56fc6f8cf07d 27 steeyeTIf0tHuMWCSd7Sj4GAFSY Fig 8 ------- COMMENT: 2ffb56fc6f8cf07d 28 steeyeTIf0tHuMWCSd7Sj4GAFSY Fig 8 ------- COMMENT: 2ffb56fc6f8cf07d 29 steeyeTIf0tHuMWCSd7Sj4GAFSY Fig 8 ------- COMMENT: 2ffb56fc6f8cf07d 30 steeyeTIf0tHuMWCSd7Sj4GAFSY Fig 8 ------- COMMENT: 2ffb56fc6f8cf07d 31 steeyeTIf0tHuMWCSd7Sj4GAFSY Fig 8 ------- COMMENT: 2fff4fd823170b6c 1 tvbGufwjMdfRdAMoJ+lpF6LLvT4 Since Ltc1 re- quires EMC for its localization and function at ER-Plasma mem- brane and ER-mitochondria contact sites (Figure 3), we conclude that EMC regulates ergosterol homeostasis through the ER-assisted biogenesis of the sterol transfer protein Ltc1. ........These observations lead us to conclude that EMC assists membrane protein folding and insertion by direct action on client proteins (i.e., Ltc1), but also may facilitate the biogenesis of some other membrane proteins by providing optimal membrane fluidity (i.e., ERMES components). ------- COMMENT: 2fff4fd823170b6c 2 dHXhnXztJNkYD1gz9Nk7z/ikzog Likewise, the deletion of genes encoding the above predicted components of the S. pombe EMC (emc3D, emc5D and emc6D) renders a cold- sensitive phenotype too (Figure 1D). ------- COMMENT: 2fff4fd823170b6c 3 dHXhnXztJNkYD1gz9Nk7z/ikzog Likewise, the deletion of genes encoding the above predicted components of the S. pombe EMC (emc3D, emc5D and emc6D) renders a cold- sensitive phenotype too (Figure 1D). ------- COMMENT: 2fff4fd823170b6c 4 2MvCEnU2MXx6wqNJUzMH9salVBM The oca3D strain produces compromised cells that can grow at 30C but fail to grow at lower temperatures (routinely as- sessed at 20C) (Figure 1D), a cold-sensitive phenotype that may reflect the loss of membrane lipid homeostasis,16,17 energy homeostasis,18,19 or other adaptive mechanisms required for growth at these environmental conditions. ------- COMMENT: 2fff4fd823170b6c 6 jevjmI9V6k5N3kIvYpSkWLpLBL0 As expected for an EMC component, in vivo fluorescence microscopy shows that Oca3-mCherry localizes to the ER (Figure 1A), co-localizing with the ER-reporter ADEL-GFP. ------- COMMENT: 2fff4fd823170b6c 7 5iabJxHiEHQMt4ZjaChKf+Lup1I Localization of the Tts1-mCherry construct, enriched in the tubular structure of the ER14 indicates that the ER, ER-associated plasma membrane (PM) and nuclear membrane remain unaltered in oca3-null cells (the oca3D dele- tion strain) (Figure 1B). ------- COMMENT: 2fff4fd823170b6c 8 x6gIKnNbgwlYN/HpKoGUp+MlZJg Like Oca3, localization of fluorescently tagged constructs of the putative orthologous S. pombe Emc3 (Pombase: ID SPBC1711.03), Emc5 (Pombase: ID SPAP4C9.02) and Emc6 (Pombase: ID SPCC1020.11c) is consistent with the ER structure in all these EMC subunits. ------- COMMENT: 2fff4fd823170b6c 9 x6gIKnNbgwlYN/HpKoGUp+MlZJg Like Oca3, localization of fluorescently tagged constructs of the putative orthologous S. pombe Emc3 (Pombase: ID SPBC1711.03), Emc5 (Pombase: ID SPAP4C9.02) and Emc6 (Pombase: ID SPCC1020.11c) is consistent with the ER structure in all these EMC subunits. ------- COMMENT: 2fff4fd823170b6c 10 x6gIKnNbgwlYN/HpKoGUp+MlZJg Like Oca3, localization of fluorescently tagged constructs of the putative orthologous S. pombe Emc3 (Pombase: ID SPBC1711.03), Emc5 (Pombase: ID SPAP4C9.02) and Emc6 (Pombase: ID SPCC1020.11c) is consistent with the ER structure in all these EMC subunits. ------- COMMENT: 2fff4fd823170b6c 11 mUwG08Hb3MGtODNst63xFAk977k As shown in Figure 1C, depletion of Oca3 leads to loss of Emc3, ag- gregation of Emc5, and normal ER localization, but reduced fluo- rescence levels of EMC6, revealing the requirement of Oca3 for the assembly of a functional EMC complex. ------- COMMENT: 2fff4fd823170b6c 12 ahNgFlUQIjcYh+lIGiRoEFUQ1FI (comment: CHECK XXXXXX CHANGE TO ABOLISHED XXXXXXX) As shown in Figure 1C, depletion of Oca3 leads to loss of Emc3, ag- gregation of Emc5, and normal ER localization, but reduced fluo- rescence levels of EMC6, revealing the requirement of Oca3 for the assembly of a functional EMC complex. ------- COMMENT: 2fff4fd823170b6c 13 mUwG08Hb3MGtODNst63xFAk977k As shown in Figure 1C, depletion of Oca3 leads to loss of Emc3, ag- gregation of Emc5, and normal ER localization, but reduced fluo- rescence levels of EMC6, revealing the requirement of Oca3 for the assembly of a functional EMC complex. ------- COMMENT: 2fff4fd823170b6c 14 dHXhnXztJNkYD1gz9Nk7z/ikzog Likewise, the deletion of genes encoding the above predicted components of the S. pombe EMC (emc3D, emc5D and emc6D) renders a cold- sensitive phenotype too (Figure 1D). ------- COMMENT: 2fff4fd823170b6c 15 uA5nZYzXfhYNlnGYTBRJjgYk/jA however, clumps of mitochondrial DNA (mtDNA) were present in the cytoplasm of these mutant cells (Figure 2A). ------- COMMENT: 2fff4fd823170b6c 16 24uPhlah4nDm7ppzmsfK34LeBCw Quantitative PCR analysis of target mtDNA sequences deter- mined a 28% reduction in mtDNA molecules per cell in the mutant strain at 30C, exacerexacerbated at low temperature (37% reduction at 20C) (Figure 2B). ------- COMMENT: 2fff4fd823170b6c 17 kdUvy/VNHTxr9jjVkAYFJualTQM In comparison to wild-type cells, Oca3/Emc2 depletion results in a condensed mitochondrial structure that co-localize with abnormal mtDNA aggregations (Figure 2C). ------- COMMENT: 2fff4fd823170b6c 18 rXxO4Iq/RZxGiHExxHO4btnPEdA The accumulation of reactive oxygen species (ROS) is associ- ated to mitochondrial dysfunctions.18 Remarkably, proliferating Oca3-depleted cells (at 30C) reach ROS levels similar to those found in wild-type cells subjected to oxidative stress (H2O2 treat- ment), indicating that EMC-deficient cells suffer mitochondrial stress (Figures S1A and S1B). ------- COMMENT: 2fff4fd823170b6c 19 PdULYhJynWiR7TbkocYrk/57RX0 As shown in Figure 3, Mdm34-GFP co-localizes with mitochondrial membrane markers in wild-type cells, enriched at the ERMES ER-mitochondrial contact sites. ------- COMMENT: 2fff4fd823170b6c 20 dVPyNqzvduXzYlQF5d2mDs5Z0TM In oca3D cells, most of the Mdm34-GFP is delocalized into the abnormal mito- chondria, indicating that EMC disfunction may interfere the as- sembly of ERMES components. ------- COMMENT: 2fff4fd823170b6c 21 kWhLLiYzidVXbxl61BFPuaNhMMM In agreement with its localization in ER-PM and ER-vesicles previously described in fission yeast,17 Ltc1-GFP localizes to cortical and internal dots in the ER in wild-type cells (arrows in Figure 3), but MitoHealth co-localization indicates that this pro- tein is also associated to ER-Mitochondrial contact sites in S. pombe cells (Figures 3 and S2), as reported in S. cerevisiae cells.24 ------- COMMENT: 2fff4fd823170b6c 22 kWhLLiYzidVXbxl61BFPuaNhMMM In agreement with its localization in ER-PM and ER-vesicles previously described in fission yeast,17 Ltc1-GFP localizes to cortical and internal dots in the ER in wild-type cells (arrows in Figure 3), but MitoHealth co-localization indicates that this pro- tein is also associated to ER-Mitochondrial contact sites in S. pombe cells (Figures 3 and S2), as reported in S. cerevisiae cells.24 ------- COMMENT: 2fff4fd823170b6c 23 Q1qt4tUYng6f6DZmoFIoJAoU4z0 In contrast to Mdm34-GFP, Ltc1-GFP lacks its normal ER-PM and ER-mitochondria localization (see Figure 3). This result indicates that Ltc1 localization is fully dependent on EMC activity. ------- COMMENT: 2fff4fd823170b6c 24 EBklhewhRe8DFbKfZUJVG2I/620 As shown in Figure 4A, loss of EMC results in a marked increase in the ergosterol content in the oca3D mutant strain at both temperatures when compared to levels in wild-type cells. ------- COMMENT: 2fff4fd823170b6c 25 JfGI1KYVz+eGD5jJsRpgpRKNi8w As expected for the observed ergosterol overaccumulation (Figures 4A and 4B), the growth of Oca3/Emc2-depleted cells is sensitive to nystatin and resistant to ketoconazole (Figure 4C). ------- COMMENT: 2fff4fd823170b6c 26 JfGI1KYVz+eGD5jJsRpgpRKNi8w As expected for the observed ergosterol overaccumulation (Figures 4A and 4B), the growth of Oca3/Emc2-depleted cells is sensitive to nystatin and resistant to ketoconazole (Figure 4C). ------- COMMENT: 2fff4fd823170b6c 27 HdtcL5AeDlfeWrmkMwvzZRa52wU Since Ltc1 re- quires EMC for its localization and function at ER-Plasma mem- brane and ER-mitochondria contact sites (Figure 3), we conclude that EMC regulates ergosterol homeostasis through the ER-assisted biogenesis of the sterol transfer protein Ltc1. ------- COMMENT: 2fff4fd823170b6c 28 csehFlOBc9sC/adb79hZARQ/E54 Thus, as previously reported in S. cerevisiae cells,24 this protein may also facilitate ergosterol transfer from the ER to the mitochondria. Since Ltc1 depletion leads to ergosterol overaccumulation in S. pombe cells,17 it is likely that sterol transport from the PM to the ER and from the ER to the mitochondria by this protein is required to drain ergosterol excess in these cells. ------- COMMENT: 2fff4fd823170b6c 29 w2c17VzRNDCPj53jLGvoyqTNiGc In contrast, BiP1-driven expression of mCherry-ADEL is greatly induced in Oca3/Emc2-depleted cells. The UPR senses the protein folding capacity of the ER,45 suggesting that EMC dysfunction may provoke the accumulation of ER-unfolded proteins in S. pombe cells (ER stressed cells). ------- COMMENT: 3028eeb2a1aae09f 1 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 3028eeb2a1aae09f 2 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 3028eeb2a1aae09f 3 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 3028eeb2a1aae09f 4 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 3028eeb2a1aae09f 5 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 3028eeb2a1aae09f 6 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 3028eeb2a1aae09f 7 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 3028eeb2a1aae09f 8 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 3028eeb2a1aae09f 9 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 3028eeb2a1aae09f 10 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 3028eeb2a1aae09f 11 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 3028eeb2a1aae09f 12 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 3028eeb2a1aae09f 13 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 3028eeb2a1aae09f 14 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 3028eeb2a1aae09f 15 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 3028eeb2a1aae09f 16 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 3028eeb2a1aae09f 17 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 3028eeb2a1aae09f 18 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 3028eeb2a1aae09f 19 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 3028eeb2a1aae09f 20 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 3028eeb2a1aae09f 21 1oHD8Jb8ziV38S6d+LuTML1g5ak Fig. 3A and D ------- COMMENT: 3028eeb2a1aae09f 22 VDN5sFCJhvYM6J/tU0KeDfKnwH8 Fig. 3B and D ------- COMMENT: 3028eeb2a1aae09f 23 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 3028eeb2a1aae09f 24 VDN5sFCJhvYM6J/tU0KeDfKnwH8 Fig. 3B and D ------- COMMENT: 3028eeb2a1aae09f 25 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 3028eeb2a1aae09f 26 1oHD8Jb8ziV38S6d+LuTML1g5ak Fig. 3A and D ------- COMMENT: 3028eeb2a1aae09f 27 VDN5sFCJhvYM6J/tU0KeDfKnwH8 Fig. 3B and D ------- COMMENT: 3028eeb2a1aae09f 28 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 3028eeb2a1aae09f 29 VDN5sFCJhvYM6J/tU0KeDfKnwH8 Fig. 3B and D ------- COMMENT: 3028eeb2a1aae09f 30 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 3028eeb2a1aae09f 31 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 3028eeb2a1aae09f 32 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 3028eeb2a1aae09f 33 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 3028eeb2a1aae09f 34 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 3028eeb2a1aae09f 35 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 3028eeb2a1aae09f 36 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 3028eeb2a1aae09f 37 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 3028eeb2a1aae09f 38 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 3028eeb2a1aae09f 39 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 3028eeb2a1aae09f 40 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 3028eeb2a1aae09f 41 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 3028eeb2a1aae09f 42 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 3028eeb2a1aae09f 43 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 3028eeb2a1aae09f 44 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 3028eeb2a1aae09f 45 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 3028eeb2a1aae09f 46 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 3028eeb2a1aae09f 47 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 3028eeb2a1aae09f 48 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 3028eeb2a1aae09f 49 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 3028eeb2a1aae09f 50 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 3028eeb2a1aae09f 51 /y/lT91toEt8826JxjLWsOWE/wk Fig. 5C and D ------- COMMENT: 3028eeb2a1aae09f 52 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 3058c7d21384f30e 2 At6UHI6s6QljNalTLY5uPGKG444 (comment: CHECK C868T (nt)) ------- COMMENT: 309444609ae01ccf 14 4u+It+HCzWy3xkMhs4VRwUBtpJw (comment: CHECK same as snf22delta alone) ------- COMMENT: 309444609ae01ccf 15 4u+It+HCzWy3xkMhs4VRwUBtpJw same as snf22delta alone ------- COMMENT: 309444609ae01ccf 16 4u+It+HCzWy3xkMhs4VRwUBtpJw (comment: CHECK same as snf22delta alone) ------- COMMENT: 30da765280e4ffe0 4 EE2/EzKzOIUWZx9SLk0ffnZt09U (comment: CHECK actually ectopic expression, throughout cell cycle) ------- COMMENT: 30da765280e4ffe0 5 EE2/EzKzOIUWZx9SLk0ffnZt09U (comment: CHECK actually ectopic expression, throughout cell cycle) ------- COMMENT: 30da765280e4ffe0 22 46z+BNLS1ZLrjP7eZJYXoxdlHtc (comment: normal binding periodicity over cell cycle) ------- COMMENT: 30da765280e4ffe0 23 46z+BNLS1ZLrjP7eZJYXoxdlHtc (comment: normal binding periodicity over cell cycle) ------- COMMENT: 30da765280e4ffe0 27 46z+BNLS1ZLrjP7eZJYXoxdlHtc (comment: CHECK normal binding periodicity over cell cycle) ------- COMMENT: 30e1bdcc27bfaabd 1 rChcOss2Zee+9OzcUKQSzASEwUY ------- COMMENT: 30e4ef054e6bbaf7 2 PyBvoCbcN5po2HMVdT0h4YvQ+Q0 (comment: also supported by complementation of S.c. deletion) ------- COMMENT: 30e8708205f74a74 1 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 30e8708205f74a74 2 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 30e8708205f74a74 3 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 30e8708205f74a74 4 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 30e8708205f74a74 5 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 30e8708205f74a74 6 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 30e8708205f74a74 8 9Xb+uz4ftlqagKGp7TY1gJc7WdA Fig. 4F ------- COMMENT: 310131206c5ef65d 1 zBksAxetkV4mdp7OucfBWdTkuT0 Figure 1A, 2A ------- COMMENT: 310131206c5ef65d 2 zBksAxetkV4mdp7OucfBWdTkuT0 Figure 1A, 2A ------- COMMENT: 310131206c5ef65d 8 7RVMzXxzF3fuHFWq+Y5Jtp2xV/M Figure 5, I, J, and L ------- COMMENT: 310131206c5ef65d 9 7RVMzXxzF3fuHFWq+Y5Jtp2xV/M Figure 5, I, J, and L ------- COMMENT: 310131206c5ef65d 10 7RVMzXxzF3fuHFWq+Y5Jtp2xV/M Figure 5, I, J, and L ------- COMMENT: 310131206c5ef65d 11 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 310131206c5ef65d 12 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 310131206c5ef65d 13 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 310131206c5ef65d 14 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 310b16bb1a103044 10 UB5FeVym90Jfo20tEUX3UpwbbmQ Fig. 1d and Fig. 1h ------- COMMENT: 310b16bb1a103044 11 l4QrY5eS8wMHlI9XFo3+D6IIy1c Fig. 1f ------- COMMENT: 310b16bb1a103044 12 2TEKmOz7GhGJD2tODPMTgVrNk2Y Fig. 1h ------- COMMENT: 3117b5347cb47cf6 15 x7Dns7n9WmS0NYHWDLzEMl7Jsxk Fig. 2C,D ------- COMMENT: 3117b5347cb47cf6 19 gbsEZDL2WsTbMBJ8nTkh0vGNYlA fig 2A ------- COMMENT: 3117b5347cb47cf6 26 XiKZWlmMPHFpOa7JxFYXS5RZY1Q fig 3 d ------- COMMENT: 3117b5347cb47cf6 28 rw3+CQ790gO4ft2BcYE/3+1h21k Fig. 4E reduced by >70% ------- COMMENT: 3117b5347cb47cf6 30 VQ82RkxHnp8pv5ua6o4SvLevN7w Fig. 4C,D ------- COMMENT: 3117b5347cb47cf6 32 C5DT7h4YvhaLVP2PQ2NxOMkC+IA Fig. 4A,B ------- COMMENT: 3117b5347cb47cf6 33 5DWqo03puFeQtodWqcCbmJ6nfyM fig 1A ------- COMMENT: 3117b5347cb47cf6 34 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: 3117b5347cb47cf6 36 9RRJbapBkUjmsTpCAr/daHqYlgU Fig. 1C–E; (comment: Type I) ------- COMMENT: 3117b5347cb47cf6 37 9RRJbapBkUjmsTpCAr/daHqYlgU Fig. 1C–E; (comment: Type I) ------- COMMENT: 3117b5347cb47cf6 38 9RRJbapBkUjmsTpCAr/daHqYlgU Fig. 1C–E; (comment: Type I) ------- COMMENT: 3117b5347cb47cf6 39 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: 3117b5347cb47cf6 40 0xqxhq7YMvudoPM5i3C2gmOIWXE (supplementary material Fig. S1A ------- COMMENT: 3117b5347cb47cf6 43 N3IrWkN6FUtHfTt6JCaaWP4L+8c fig 2 A ------- COMMENT: 3117b5347cb47cf6 44 N3IrWkN6FUtHfTt6JCaaWP4L+8c fig 2 A ------- COMMENT: 3117b5347cb47cf6 45 274moqD3MOhWPA6Es/G5uFcvPK0 fig S2B ------- COMMENT: 3117b5347cb47cf6 46 274moqD3MOhWPA6Es/G5uFcvPK0 fig S2B ------- COMMENT: 3117b5347cb47cf6 47 QghTkgBadiIs5dvIJaBLttnmnFc Overall, our data suggest that Klp5–Klp6 delivers PP1 to the attached kinetochores, thereby promoting SAC silencing. ------- COMMENT: 3117b5347cb47cf6 48 QghTkgBadiIs5dvIJaBLttnmnFc Overall, our data suggest that Klp5–Klp6 delivers PP1 to the attached kinetochores, thereby promoting SAC silencing. ------- COMMENT: 3117b5347cb47cf6 49 y737wHNeL2Py9ILPobTfFfFsKZE In any case, the results shown here imply that Klp5–Klp6 localises to the kinetochores through interaction with the Alp7–Alp14 complex. ------- COMMENT: 3117b5347cb47cf6 50 y737wHNeL2Py9ILPobTfFfFsKZE In any case, the results shown here imply that Klp5–Klp6 localises to the kinetochores through interaction with the Alp7–Alp14 complex. ------- COMMENT: 3117b5347cb47cf6 51 cWU1gxrBhSdpW25jqWFTdTZ9qBA fig 3A ------- COMMENT: 3117b5347cb47cf6 52 cWU1gxrBhSdpW25jqWFTdTZ9qBA fig 3A ------- COMMENT: 3117b5347cb47cf6 53 cWU1gxrBhSdpW25jqWFTdTZ9qBA fig 3A ------- COMMENT: 3117b5347cb47cf6 54 cWU1gxrBhSdpW25jqWFTdTZ9qBA fig 3A ------- COMMENT: 3117b5347cb47cf6 55 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: 3117b5347cb47cf6 56 BmZkCiNrR5Syc8Af3Z3TzLdln2s Fig. 1C; Fig. 3D ------- COMMENT: 3117b5347cb47cf6 57 BmZkCiNrR5Syc8Af3Z3TzLdln2s Fig. 1C; Fig. 3D ------- COMMENT: 3117b5347cb47cf6 60 rw3+CQ790gO4ft2BcYE/3+1h21k Fig. 4E reduced by >70% ------- COMMENT: 3117b5347cb47cf6 62 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 3117b5347cb47cf6 63 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 3117b5347cb47cf6 64 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 3117b5347cb47cf6 65 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: 3117b5347cb47cf6 66 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: 31248641de1cbfdc 4 Oz4GFaNCnWMcfz1jx1AZrS/l++4 (comment: Forms gamma H2A dependent nuclear foci when over-expressed) ------- COMMENT: 312c8af6293e302e 1 Om4DPRoJEld/1ik18zc9e7c/W64 Fig5. Asc1 colocalized with stress granule proteins in response to heat shock. ------- COMMENT: 312c8af6293e302e 2 e6EIXzaQHPWiz38UXWX9zUg5UKs (comment: CHECK Asc1 binds to gus1) ------- COMMENT: 312c8af6293e302e 3 KfDQQHByJMCn5UgI9SPR6zwrJ8k (comment: CHECK Asc1 binds to rar1) ------- COMMENT: 312c8af6293e302e 4 CRfrP4OjTJbYgqpYn5miQQXMvug (comment: CHECK Asc1 binds to tif32) ------- COMMENT: 312c8af6293e302e 5 MUoDCJFRza7DQOwSNk3ajvv1At4 (comment: CHECK Asc1 interactes with tif32) ------- COMMENT: 312c8af6293e302e 6 WClZqzCjVtylcap7d9bxKFAkmbI (comment: CHECK Asc1 interacts with tif211) ------- COMMENT: 312c8af6293e302e 7 qfkI4QZGjxFOievgbJXDMEwf+yc (comment: CHECK Asc1 interacts with rps001) ------- COMMENT: 312c8af6293e302e 8 olXcT/43x8ZEW9o6uQ+5xXGUo+o (comment: CHECK Asc1 form complex with rar1 and gus1) ------- COMMENT: 312c8af6293e302e 9 olXcT/43x8ZEW9o6uQ+5xXGUo+o (comment: CHECK Asc1 form complex with rar1 and gus1) ------- COMMENT: 312c8af6293e302e 11 hnSdu+5QyVoKNqS3wIhIFXqlDp8 (comment: CHECK Asc1 binds to rar1 and gus1) ------- COMMENT: 312c8af6293e302e 12 xOFcx1ignXmd85n8tuAdUVIxLrk (comment: CHECK Asc1 binds to rar1 and gus1) ------- COMMENT: 312c8af6293e302e 14 cROJhx0N1y01KaKmqXqKmpJDG6Y (comment: CHECK Asc1 interacta with rps001) ------- COMMENT: 312c8af6293e302e 19 4by5A5QoxcrE26gML9K04nPoqgQ (comment: CHECK N-terminal of asc1 interacts with gus1) ------- COMMENT: 312c8af6293e302e 26 EaJ1cIJCgxH1h7dmT6mEA3YTf4A Figure 1d ------- COMMENT: 312c8af6293e302e 27 CFnQxPG6p9ywfLGuxKEmPsOSKeQ Figure 1e ------- COMMENT: 312c8af6293e302e 28 hkAYTwXptZJW0cTNkvnkV5Y/U5s Figure 2d ------- COMMENT: 312c8af6293e302e 29 hkAYTwXptZJW0cTNkvnkV5Y/U5s Figure 2d ------- COMMENT: 312c8af6293e302e 30 hkAYTwXptZJW0cTNkvnkV5Y/U5s Figure 2d ------- COMMENT: 312c8af6293e302e 31 DRYJSGGCd2yYeqANMtUVaEW/nts Figure 3a ------- COMMENT: 312c8af6293e302e 34 DRYJSGGCd2yYeqANMtUVaEW/nts Figure 3a ------- COMMENT: 312c8af6293e302e 35 iN1JInTAzN/QB1QYBsM4gxOjMdw Figure 3e ------- COMMENT: 312c8af6293e302e 36 iN1JInTAzN/QB1QYBsM4gxOjMdw Figure 3e ------- COMMENT: 312c8af6293e302e 37 ykwt1kl6fVRGox2mPIE4fPw1TiY (comment: CHECK cytoplasmic translation is a parent to this term) ------- COMMENT: 312c8af6293e302e 38 ybGeJolpcZQK0juJBL5ki2kxBUU Fig.2 (comment: Asc1 associates with polysomes.) ------- COMMENT: 312c8af6293e302e 39 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig. 4 ------- COMMENT: 312c8af6293e302e 40 MB+WxZqtLoWkmNqZLfwtd2DBEh8 fig. 4h ------- COMMENT: 312c8af6293e302e 41 MB+WxZqtLoWkmNqZLfwtd2DBEh8 fig. 4h ------- COMMENT: 312c8af6293e302e 42 wd/ZbuqMwrYnhSdbyjisJhJW4ng COMMENT: 312c8af6293e302e 41 MB+WxZqtLoWkmNqZLfwtd2DBEh8 fig 6 ------- COMMENT: 312c8af6293e302e 44 IrL1crk8yj/EvSb4KfMbBwt+eHM COMMENT: 312c8af6293e302e 41 MB+WxZqtLoWkmNqZLfwtd2DBEh8 fig 6f ------- COMMENT: 31390a94c8f8643a 1 O3ylP06i8J8/hl6hxxf7B0yiVYA (comment: G2 arrest shown by FACS analysis) ------- COMMENT: 31390a94c8f8643a 2 5BRJ+a271SX/unuqmyGLsGdg5Ds (comment: crosses with this mutant generate a high level of diploids. ------- COMMENT: 31390a94c8f8643a 3 O3ylP06i8J8/hl6hxxf7B0yiVYA (comment: G2 arrest shown by FACS analysis) ------- COMMENT: 31390a94c8f8643a 4 gkmQCcdh2dlbcnVSnOjDQeOs/bI cells inviable at all temperatures in presence of wee1+ ------- COMMENT: 31390a94c8f8643a 5 CHoctUaireVq1tBA9JFvAaiLCjA (comment; HI used as substrate) ------- COMMENT: 31390a94c8f8643a 6 AN41slV73ROTI4ZD/8pBQPTBwNA (comment: CHECK cdc2-A21 suppresses mitotic catastrophe at high temperature) ------- COMMENT: 31390a94c8f8643a 7 lw2jdCi0Rn2ga603X5u25QNblRw (comment: cdc2-E8 suppresses mitotic catastrophe at high temperature) ------- COMMENT: 31390a94c8f8643a 8 Vis4hrESX+QuuKmEsLVylGGwxWY (comment: cdc2-E9 suppresses mitotic catastrophe at high temperature) ------- COMMENT: 31390a94c8f8643a 9 SxUD5iaenKTY/MZMECrFtAGsjRA (comment: Hi used as substrate) ------- COMMENT: 31390a94c8f8643a 10 CHoctUaireVq1tBA9JFvAaiLCjA (comment: HI used as substrate) ------- COMMENT: 31390a94c8f8643a 11 SxUD5iaenKTY/MZMECrFtAGsjRA (comment: Hi used as substrate) ------- COMMENT: 3154c42f677dce94 1 XitM7mCzJch6HZbgPvLJZYCEdZA Fig S4a ------- COMMENT: 3154c42f677dce94 2 XitM7mCzJch6HZbgPvLJZYCEdZA Fig S4a ------- COMMENT: 3154c42f677dce94 3 XitM7mCzJch6HZbgPvLJZYCEdZA Fig S4a ------- COMMENT: 3154c42f677dce94 4 XitM7mCzJch6HZbgPvLJZYCEdZA Fig S4a ------- COMMENT: 3154c42f677dce94 5 XitM7mCzJch6HZbgPvLJZYCEdZA Fig S4a ------- COMMENT: 3154c42f677dce94 6 XitM7mCzJch6HZbgPvLJZYCEdZA Fig S4a ------- COMMENT: 3154c42f677dce94 7 XitM7mCzJch6HZbgPvLJZYCEdZA Fig S4a ------- COMMENT: 3154c42f677dce94 8 XitM7mCzJch6HZbgPvLJZYCEdZA Fig S4a ------- COMMENT: 3154c42f677dce94 9 XitM7mCzJch6HZbgPvLJZYCEdZA Fig S4a ------- COMMENT: 3154c42f677dce94 10 XitM7mCzJch6HZbgPvLJZYCEdZA Fig S4a ------- COMMENT: 3154c42f677dce94 11 XitM7mCzJch6HZbgPvLJZYCEdZA Fig S4a ------- COMMENT: 3154c42f677dce94 12 XitM7mCzJch6HZbgPvLJZYCEdZA Fig S4a ------- COMMENT: 3154c42f677dce94 13 XitM7mCzJch6HZbgPvLJZYCEdZA Fig S4a ------- COMMENT: 3154c42f677dce94 14 XitM7mCzJch6HZbgPvLJZYCEdZA Fig S4a ------- COMMENT: 3154c42f677dce94 15 XitM7mCzJch6HZbgPvLJZYCEdZA Fig S4a ------- COMMENT: 3154c42f677dce94 16 XitM7mCzJch6HZbgPvLJZYCEdZA Fig S4a ------- COMMENT: 3154c42f677dce94 19 MoB/xAx8HWHJ5Zou0PMGR2JLaRo (Fig 1F) ------- COMMENT: 3154c42f677dce94 20 GKf6tV7oqFclFsVFJi7uNThL+Fo fig 1F ------- COMMENT: 3154c42f677dce94 21 y08pfUMhZIGsxcQwYZ6lUTdM3FI Fig 1F ------- COMMENT: 3154c42f677dce94 22 y08pfUMhZIGsxcQwYZ6lUTdM3FI Fig 1F ------- COMMENT: 3154c42f677dce94 23 mKmn5gYQWlwc9yx+EDT22gD+cO4 Fig 1A, C–E ------- COMMENT: 3154c42f677dce94 24 mKmn5gYQWlwc9yx+EDT22gD+cO4 Fig 1A, C–E ------- COMMENT: 3154c42f677dce94 25 GnSGXeHwG9ypB9gbwG1U5xE1GyA Fig S4A ------- COMMENT: 3154c42f677dce94 26 GnSGXeHwG9ypB9gbwG1U5xE1GyA Fig S4A ------- COMMENT: 3154c42f677dce94 27 h/C007xWt+TZ47FmWvRdlbdjlfU Fig1 C,D ------- COMMENT: 3154c42f677dce94 28 l+ClTEucYvRTKQk0DuGrdf0tCXw Fig 1I–L ------- COMMENT: 3154c42f677dce94 29 l+ClTEucYvRTKQk0DuGrdf0tCXw Fig 1I–L ------- COMMENT: 3154c42f677dce94 30 l+ClTEucYvRTKQk0DuGrdf0tCXw Fig 1I–L ------- COMMENT: 3154c42f677dce94 31 MYorVWvaIrKVKrf5pIf2kpwPQkw Fig 3B,C ------- COMMENT: 3154c42f677dce94 32 MYorVWvaIrKVKrf5pIf2kpwPQkw Fig 3B,C ------- COMMENT: 3154c42f677dce94 33 Q4G0UgOhrxeQ/BH8vqLtaie6VO8 Fig 3D ------- COMMENT: 3154c42f677dce94 34 iugwOICngLmkyma0auuN7ZPWnmQ Fig 3D ------- COMMENT: 3154c42f677dce94 35 bvZvTAYQ070diUubOxrit7FbGe8 Fig S1B ------- COMMENT: 3154c42f677dce94 36 bvZvTAYQ070diUubOxrit7FbGe8 Fig S1B ------- COMMENT: 3154c42f677dce94 37 eHhpjsLRfjR/AIJpuV22JnIqQ74 Fig S1I ------- COMMENT: 3154c42f677dce94 38 eHhpjsLRfjR/AIJpuV22JnIqQ74 Fig S1I ------- COMMENT: 3154c42f677dce94 39 eHhpjsLRfjR/AIJpuV22JnIqQ74 Fig S1I ------- COMMENT: 3154c42f677dce94 40 nR5bA+oMlof5ihoUmMH4f0HctJo Fig S2F ------- COMMENT: 3154c42f677dce94 41 nR5bA+oMlof5ihoUmMH4f0HctJo Fig S2F ------- COMMENT: 3154c42f677dce94 42 dagMBG4ZG7CnCwzRqBa4a/joVL8 Fig S2C,D,E ------- COMMENT: 3154c42f677dce94 43 dagMBG4ZG7CnCwzRqBa4a/joVL8 Fig S2C,D,E ------- COMMENT: 3154c42f677dce94 44 dagMBG4ZG7CnCwzRqBa4a/joVL8 Fig S2C,D,E ------- COMMENT: 3154c42f677dce94 45 dagMBG4ZG7CnCwzRqBa4a/joVL8 Fig S2C,D,E ------- COMMENT: 3154c42f677dce94 46 nR5bA+oMlof5ihoUmMH4f0HctJo Fig S2F ------- COMMENT: 3154c42f677dce94 47 nR5bA+oMlof5ihoUmMH4f0HctJo Fig S2F ------- COMMENT: 3154c42f677dce94 48 Ag6GoP3zsrPsg4IZ5Mmwasgbws4 Fig3 ------- COMMENT: 3154c42f677dce94 49 Ag6GoP3zsrPsg4IZ5Mmwasgbws4 Fig3 ------- COMMENT: 3154c42f677dce94 50 Ag6GoP3zsrPsg4IZ5Mmwasgbws4 Fig3 ------- COMMENT: 3154c42f677dce94 51 Ag6GoP3zsrPsg4IZ5Mmwasgbws4 Fig3 ------- COMMENT: 3154c42f677dce94 52 Ag6GoP3zsrPsg4IZ5Mmwasgbws4 Fig3 ------- COMMENT: 3154c42f677dce94 53 Ag6GoP3zsrPsg4IZ5Mmwasgbws4 Fig3 ------- COMMENT: 3154c42f677dce94 54 Ag6GoP3zsrPsg4IZ5Mmwasgbws4 Fig3 ------- COMMENT: 3154c42f677dce94 55 Ag6GoP3zsrPsg4IZ5Mmwasgbws4 Fig3 ------- COMMENT: 3154c42f677dce94 56 Ag6GoP3zsrPsg4IZ5Mmwasgbws4 Fig3 ------- COMMENT: 3154c42f677dce94 57 Ag6GoP3zsrPsg4IZ5Mmwasgbws4 Fig3 ------- COMMENT: 3154c42f677dce94 58 kQNZT5YTrRMkSCs+wnAzRhhdTtw Fig S1A and D ------- COMMENT: 3154c42f677dce94 59 mKmn5gYQWlwc9yx+EDT22gD+cO4 Fig 1A, C–E ------- COMMENT: 3154c42f677dce94 60 hcQdrfy+TIgsiXcfOLfeaRWtnCQ Fig 1A, C–D ------- COMMENT: 3154c42f677dce94 61 iDKXzggnuXXRVy+AwJCI1FLfmMo Figure 1H ------- COMMENT: 3154c42f677dce94 62 HnYiak1OUkNlzQLoqjbPfVCFmxg Figure 1H ------- COMMENT: 3154c42f677dce94 63 HnYiak1OUkNlzQLoqjbPfVCFmxg Figure 1H ------- COMMENT: 3154c42f677dce94 64 wj2VSE893AMBlEWR8+9Ey55om+8 Figure 2B ------- COMMENT: 3154c42f677dce94 65 78df3FWloXZZUYl2IkEADbofP6I (Fig 2D and E) (comment: (mad1 localized did not rescue) ------- COMMENT: 3154c42f677dce94 66 czYGLvBorPA+9LSaPrnzKFUpBAU Fig 3F ------- COMMENT: 3154c42f677dce94 70 oGTzdM4Ff/5CwiyTvKraoQudm9A A complex between the checkpoint proteins Mad1 and Mad2 provides a platform for Mad2:Mad2 dimerization at unattached kinetochores, which enables Mad2 to delay anaphase. Here, we show that mutations in Bub1 and within the Mad1 C-terminal domain impair the kinetochore localization of Mad1:Mad2 and abrogate checkpoint activity. Artificial kinetochore recruitment of Mad1 in these mutants co-recruits Mad2; however, the checkpoint remains non-functional. We identify specific mutations within the C-terminal head of Mad1 that impair checkpoint activity without affecting the kinetochore localization of Bub1, Mad1 or Mad2. Hence, Mad1 potentially in conjunction with Bub1 has a crucial role in checkpoint signalling in addition to presenting Mad2. ------- COMMENT: 316171a7a08d7ce8 3 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 316171a7a08d7ce8 4 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 316171a7a08d7ce8 5 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 316171a7a08d7ce8 6 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 316171a7a08d7ce8 7 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 316171a7a08d7ce8 10 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 316171a7a08d7ce8 11 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 316171a7a08d7ce8 12 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 316171a7a08d7ce8 13 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 316171a7a08d7ce8 14 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 316171a7a08d7ce8 15 DVJMM6AvJyLz8J8dkwtY+pFizxc (Fig. 5E) ------- COMMENT: 316171a7a08d7ce8 16 DVJMM6AvJyLz8J8dkwtY+pFizxc (Fig. 5E) ------- COMMENT: 316171a7a08d7ce8 17 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 316171a7a08d7ce8 18 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 316171a7a08d7ce8 19 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 316171a7a08d7ce8 20 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 316171a7a08d7ce8 21 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 316171a7a08d7ce8 22 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 316171a7a08d7ce8 23 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 316171a7a08d7ce8 24 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 316171a7a08d7ce8 25 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 316171a7a08d7ce8 26 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 316171a7a08d7ce8 27 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 316171a7a08d7ce8 28 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 316171a7a08d7ce8 29 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 316171a7a08d7ce8 30 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 316171a7a08d7ce8 31 xqjTztL6qGqaPgp6WgcG+iOomGI (Fig. S1B) ------- COMMENT: 316171a7a08d7ce8 32 xqjTztL6qGqaPgp6WgcG+iOomGI (Fig. S1B) ------- COMMENT: 316171a7a08d7ce8 33 xqjTztL6qGqaPgp6WgcG+iOomGI (Fig. S1B) ------- COMMENT: 316171a7a08d7ce8 34 xqjTztL6qGqaPgp6WgcG+iOomGI (Fig. S1B) ------- COMMENT: 316171a7a08d7ce8 35 d2NdxnatvYWNnh9afhcOiSOs0XA (Fig. S1D) ------- COMMENT: 316171a7a08d7ce8 36 d2NdxnatvYWNnh9afhcOiSOs0XA (Fig. S1D) ------- COMMENT: 316171a7a08d7ce8 37 d2NdxnatvYWNnh9afhcOiSOs0XA (Fig. S1D) ------- COMMENT: 316171a7a08d7ce8 38 d2NdxnatvYWNnh9afhcOiSOs0XA (Fig. S1D) ------- COMMENT: 316171a7a08d7ce8 39 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 316171a7a08d7ce8 40 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 316171a7a08d7ce8 41 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 316171a7a08d7ce8 42 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 316171a7a08d7ce8 43 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 316171a7a08d7ce8 44 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 316171a7a08d7ce8 45 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 316171a7a08d7ce8 46 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 316171a7a08d7ce8 47 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 316171a7a08d7ce8 48 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 316171a7a08d7ce8 49 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 316171a7a08d7ce8 50 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 316171a7a08d7ce8 51 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 316171a7a08d7ce8 52 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 316171a7a08d7ce8 53 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 316171a7a08d7ce8 54 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 316171a7a08d7ce8 55 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 316171a7a08d7ce8 56 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 316171a7a08d7ce8 57 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 316171a7a08d7ce8 58 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 316171a7a08d7ce8 59 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 316171a7a08d7ce8 60 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 316171a7a08d7ce8 61 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 316171a7a08d7ce8 62 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 316171a7a08d7ce8 63 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 316171a7a08d7ce8 64 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 316171a7a08d7ce8 65 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 316171a7a08d7ce8 66 gA4P48uKuk6ti5ukTRuiFvXo/Zw (Fig. S2C) ------- COMMENT: 316171a7a08d7ce8 67 fQxGLJwIwpyTMMv1IJ7Ao2imf6g Hhp1 and Hhp2 localize diffusely across the nucleus and cytoplasm with a concentration at SPBs,38 but they did not co-localize with Rad52-GFP foci, even when genotoxic stress was induced (Supplementary material, Figure S2D). ------- COMMENT: 316171a7a08d7ce8 68 fQxGLJwIwpyTMMv1IJ7Ao2imf6g Hhp1 and Hhp2 localize diffusely across the nucleus and cytoplasm with a concentration at SPBs,38 but they did not co-localize with Rad52-GFP foci, even when genotoxic stress was induced (Supplementary material, Figure S2D). ------- COMMENT: 316171a7a08d7ce8 69 XG3eoms4RFZnC4bn4IoxlfXlZaw These data strongly suggest that S60 is an additional Hhp1 and Hhp2 phosphorylation site, and that S60, S62, S75, and S87 are the primary sites on Arp8 that are targeted by Hhp1 and Hhp2. ------- COMMENT: 316171a7a08d7ce8 70 DVJMM6AvJyLz8J8dkwtY+pFizxc (Fig. 5E) ------- COMMENT: 316171a7a08d7ce8 71 DVJMM6AvJyLz8J8dkwtY+pFizxc (Fig. 5E) ------- COMMENT: 316171a7a08d7ce8 72 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: 316171a7a08d7ce8 73 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: 316171a7a08d7ce8 74 JT3k9eYP2DeBwb6gtqzco0jbS2M (Fig. 5G) ------- COMMENT: 316171a7a08d7ce8 75 JT3k9eYP2DeBwb6gtqzco0jbS2M (Fig. 5G) ------- COMMENT: 316171a7a08d7ce8 76 U28uRRiodUY3Y1KTMp42IidYEug (Fig. 5H) ------- COMMENT: 316171a7a08d7ce8 77 U28uRRiodUY3Y1KTMp42IidYEug (Fig. 5H) ------- COMMENT: 31a2874ab03c580a 1 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 31a2874ab03c580a 5 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 31a2874ab03c580a 6 cpDt11OX2lapwEyGYhWF0kdInw4 GFP-Lid2 is resistant to detergent extraction indicating Lid2 is a chromatin-binding protein (Figure 2A). ------- COMMENT: 31a2874ab03c580a 7 HHBjQ4F/lJO4aDJMxDqG1JPAY74 GFP-Lid2 is resistant to detergent extraction indicating Lid2 is a chromatin-binding protein (Figure 2A).showed enrichment of DNA from centromeres and the mating-type region, indicating Lid2 is associated with heterochromatin (Figure 2B). ------- COMMENT: 31a2874ab03c580a 8 HHBjQ4F/lJO4aDJMxDqG1JPAY74 GFP-Lid2 is resistant to detergent extraction indicating Lid2 is a chromatin-binding protein (Figure 2A).showed enrichment of DNA from centromeres and the mating-type region, indicating Lid2 is associated with heterochromatin (Figure 2B). ------- COMMENT: 31a2874ab03c580a 9 01SHU2px/GMRCfYp8IiLX+eI7B0 We deleted one copy of lid2+ by kanamycin reporter gene replacement (kan+) in a WT diploid strain (lid2+/lid2Δ::kan+) and tetrad analysis was performed after sporulation. Only two germinating spores from a tetrad were viable, confirming that lid2+ is an essential gene ------- COMMENT: 31a2874ab03c580a 10 BYsX1aYTx0MBigOy5WE2iuGYf+E WT and frequently exhibited an aberrant elongated cell shape (Figure 2C) ------- COMMENT: 31a2874ab03c580a 12 /9hgM9krpuOasVTiGUhqW2I89/8 28% of the cells contained fragmented nuclear DNA (Figure 2D), indicating that the mutant nucleus is disorganized. ------- COMMENT: 31a2874ab03c580a 13 7pntNtNla0HfRmOlPWOV5LNNmMA As shown in Figure 2E, lid2-j, like clr8Δ, is hypersensitive to TBZ ------- COMMENT: 31a2874ab03c580a 14 yqzjT4nu2gA4iU3/hs5tLf+tBhA We found that deletion of the Lid2 JmjC domain resulted in the complete loss of ura4+ silencing at both the otr and imr loci (Figure 3A and Figure 7A). ------- COMMENT: 31a2874ab03c580a 15 yqzjT4nu2gA4iU3/hs5tLf+tBhA We found that deletion of the Lid2 JmjC domain resulted in the complete loss of ura4+ silencing at both the otr and imr loci (Figure 3A and Figure 7A). ------- COMMENT: 31a2874ab03c580a 16 glfse4i0U5HSIiOunRpVSoLC6oA mating-type region was likewise reduced (Figure 3A) ------- COMMENT: 31a2874ab03c580a 17 73kWrWXlSfmKhDuhUYnghFpiHbE As shown in Figure 3B, H3K9 methylation at the centromere was completely abolished. ------- COMMENT: 31a2874ab03c580a 18 kPcM45rdPcXSh7+C3V/um7q3cwI In contrast, H3K4me3 methylation was increased significantly (Figure 3C) ------- COMMENT: 31a2874ab03c580a 19 f29vLHTC14BfN10/hNOZHgJyT4k drastic reduction of Swi6 binding (Figure 3D). ------- COMMENT: 31a2874ab03c580a 20 WAOBW/Op+XvhuvPcHc0UzghHIuA We then determined whether Lid2 is required for the recruitment of Clr4 to heterochromatin. We carried out a ChIP experiment using lid2-j or clr8Δ containing a N-terminal FLAG-tagged Clr4. The localization of Clr4 at centromeres is abrogated in both mutants (Figure 3E). ------- COMMENT: 31a2874ab03c580a 21 WAOBW/Op+XvhuvPcHc0UzghHIuA We then determined whether Lid2 is required for the recruitment of Clr4 to heterochromatin. We carried out a ChIP experiment using lid2-j or clr8Δ containing a N-terminal FLAG-tagged Clr4. The localization of Clr4 at centromeres is abrogated in both mutants (Figure 3E). ------- COMMENT: 31a2874ab03c580a 22 tTsG1fOtUADhEruDFTt6E1WLPhY overexpressing Lid2 enhances H3K9 methylation (Figures 5G and H) ------- COMMENT: 31a2874ab03c580a 23 qZS5C/wxDAmNUCtrFQKAAO0I6t4 H3K4me3 staining was reduced to nearly undetectable levels in 82% of the cells overexpressing Lid2, suggesting that Lid2 can specifically demethylate H3K4 me3 (Figure 4B) ------- COMMENT: 31a2874ab03c580a 25 FJcrSsVggk1YYw26oTW9M7m2Kfs forward and reverse centromeric strands were detected in lid2-j and accumulated at the same level as in clr8Δ, suggesting that Lid2 is involved in the RNAi pathway. ------- COMMENT: 31a2874ab03c580a 26 FJcrSsVggk1YYw26oTW9M7m2Kfs forward and reverse centromeric strands were detected in lid2-j and accumulated at the same level as in clr8Δ, suggesting that Lid2 is involved in the RNAi pathway. ------- COMMENT: 31a2874ab03c580a 28 JxDyrhbUu2bU3J1iw0AggagmGm4 As shown in Figure 5C, siRNA is barely detectable. ------- COMMENT: 31a2874ab03c580a 29 IioMJFIitkxDZz4iWQeVPh2bsEs As shown in Figure 5D, the association of Ago1 with the centromere is significantly reduced in lid2-j, indicating that Lid2 is required for RITS to load onto centromeres. ------- COMMENT: 31a2874ab03c580a 30 UQKE3aOlt1XDzq+ARXldlSn+2lM In WT cells, Myc- Clr8 associates with centromere otr regions, but not in lid2-j suggesting that Lid2 is required for Clr8 association with heterochromatin (Figure 5E). ------- COMMENT: 31a2874ab03c580a 31 HHBjQ4F/lJO4aDJMxDqG1JPAY74 GFP-Lid2 is resistant to detergent extraction indicating Lid2 is a chromatin-binding protein (Figure 2A).showed enrichment of DNA from centromeres and the mating-type region, indicating Lid2 is associated with heterochromatin (Figure 2B). ------- COMMENT: 31a2874ab03c580a 32 erSC+QaPzc2o6OFFiIGtFI8HhPs As shown in Figure 5E, while Lid2 accumulated at centromeres in the WT, the centromere localization of Lid2 in the clr8 mutant is significantly decreased. ------- COMMENT: 31a2874ab03c580a 33 gSvL7EXS3wpqsUJDUnxsVHh8oSQ Figure 5F, the point mutation resulted in a significant loss of silencing at the centromere otr region. ------- COMMENT: 31a2874ab03c580a 34 NYD4D+6W9bhfrgC3DQR9yCztUJ4 ChIP assays indicated that H3K9me2 methylation at the region was abolished, while H3K4me3 methylation was increased more than seven-fold (Figure S2) ------- COMMENT: 31a2874ab03c580a 35 NYD4D+6W9bhfrgC3DQR9yCztUJ4 ChIP assays indicated that H3K9me2 methylation at the region was abolished, while H3K4me3 methylation was increased more than seven-fold (Figure S2) ------- COMMENT: 31a2874ab03c580a 36 1XNO6cTJx2gsKaCwY68zjpprJnE the point mutation had little effect on the interaction of Lid2 with its interacting partners, such as Cul4 and Set1 (Figure S3 and S8). ------- COMMENT: 31a2874ab03c580a 37 1XNO6cTJx2gsKaCwY68zjpprJnE the point mutation had little effect on the interaction of Lid2 with its interacting partners, such as Cul4 and Set1 (Figure S3 and S8). ------- COMMENT: 31a2874ab03c580a 38 AqTzBgV9u8Ug3j5VghdjQa1Mbd4 We next examined Swi6 localization in the lid2-j mutant using N-terminal tagged GFP-Swi6. In WT vegetative cells, 3–4 GFP-Swi6 spots are observed. This is because the three centromeres cluster on the nuclear envelope in the vicinity of the spindle pole body whereas telomeres loosely cluster on the nuclear envelope, apart from centromeres. clr4 and clr8 mutants have a diffuse Swi6 localization due to the disruption of heterochromatin. To our surprise, we did not see the same GFP-Swi6 pattern in the lid2-j mutant as in clr8Δ. Rather, we found that 78% of the cells contain more than 5 GFP-Swi6 spots, with nearly 30% having more than 10 spots (Figure 6A). The abnormal distribution of Swi6 also can be observed in meiotic horsetail stage nuclei (Figure 6A). The aberrant Swi6 localization is not caused by defects in centromere or telomere clustering since the distribution of centromeres and telomeres, as marked by Cnp1-GFP or Taz1-GFP respectively, is unaffected in the lid2-j mutant (Figure S4). We further confirmed that telomeres cluster normally by visualizing their distribution in a lid2-j strain carrying mCherry-Swi6 and the telomere marker Taz1-GFP (Figure S4). These results suggest that heterochromatin is induced in euchromatic regions in lid2-j. ------- COMMENT: 31a2874ab03c580a 39 sfJmx+gkOC9hY5PVGguttgQo98g (comment: RNAI dependent) ------- COMMENT: 31a2874ab03c580a 40 wcFKF3WzexFZAG4C7k5codV0DJw About 50% (2665 out of 5241) of the genes in the genome were downregulated in mutant cells compared to WT (Figure 6C and Table S3), consistent with the formation of ectopic heterochromatin. ------- COMMENT: 31a2874ab03c580a 41 MVwfyGCqq7c9WBAAcHB4sqtMQk8 We further noted the striking similarity of the genome-wide transcription profiles of the lid2-j and lsd1Δ mutants (Figure 6C), suggest ------- COMMENT: 31a2874ab03c580a 43 gWiyjfV1YTLeFN2Oh1/k+Wjq7T8 In contrast to the overexpression of Lid2 alone, which leads to a dramatic decrease in H3K4me3 (Figure 4B), reduction of H3K4me3 is minimal when Set1 or Lsd1 is also overexpressed (Figure 4C). ------- COMMENT: 31a2874ab03c580a 44 VUY300/d+pP1WLE4nxjOPHq+/+E We also examined the GFP-Swi6 distribution in the mutants (Figure 7B). While the lid2-phd1 mutant is similar to the WT, the nuclei in the lid2-phd2 and lid2-phd3 mutants often contained excessive GFP- Swi6 dots, suggesting that euchromatin assembly is disrupted in the mutants (Figure 7B). ------- COMMENT: 31a2874ab03c580a 45 ttQAEk24fWdyoIbhCZ1cxglY0jo silencing at the imr region in lid2-phd2 and lid2-phd3 mutants was significantly impaired while lid2-phd1 showed only a slight defect (Figure 7A). ------- COMMENT: 31a2874ab03c580a 46 ttQAEk24fWdyoIbhCZ1cxglY0jo silencing at the imr region in lid2-phd2 and lid2-phd3 mutants was significantly impaired while lid2-phd1 showed only a slight defect (Figure 7A). ------- COMMENT: 31a2874ab03c580a 47 ttQAEk24fWdyoIbhCZ1cxglY0jo silencing at the imr region in lid2-phd2 and lid2-phd3 mutants was significantly impaired while lid2-phd1 showed only a slight defect (Figure 7A). ------- COMMENT: 31a2874ab03c580a 48 OezVLp5Dg6ogXYwzuHjWz94bkTw Obvious reduction of H3K9 methylation was also observed in lid2-phd2 and lid2-phd3 mutants (Figure S9) ------- COMMENT: 31a2874ab03c580a 49 OezVLp5Dg6ogXYwzuHjWz94bkTw Obvious reduction of H3K9 methylation was also observed in lid2-phd2 and lid2-phd3 mutants (Figure S9) ------- COMMENT: 31a2874ab03c580a 50 VUY300/d+pP1WLE4nxjOPHq+/+E We also examined the GFP-Swi6 distribution in the mutants (Figure 7B). While the lid2-phd1 mutant is similar to the WT, the nuclei in the lid2-phd2 and lid2-phd3 mutants often contained excessive GFP- Swi6 dots, suggesting that euchromatin assembly is disrupted in the mutants (Figure 7B). ------- COMMENT: 31a707ea44c981c1 2 IkAZEZUTAD/6/hubJC/NfdRxjdU (comment: inferring that residue is Y15, though not shown experimentally) ------- COMMENT: 31b52625e666e444 4 CXtsFfxBGGjIy2XS5Aym6EPTC9k (comment: CHECK inhibits) ------- COMMENT: 31b52625e666e444 14 KBWd+alGS2eQa+eOt/JZFh56tJM (comment: no mitotic spindle) ------- COMMENT: 31eee06cfedda976 2 ZdfMqBNjFZge0AkpB5c1EsSwD4M (comment: ubiquitin monmomer inhibits sst2) ------- COMMENT: 31f078678cafa180 9 GEGkfWLtfJWc5raub/pn7OJh/+E (comment: CHECK inhibited by P(1),P(5)-bis(5'-adenosyl) pentaphosphate(5-)?) ------- COMMENT: 31f83944ec9ea1a7 75 BFlYTXz3Xq5aYunqVvj4ZbG6u6c (comment: binds centromeric transcripts) ------- COMMENT: 3248946eca150af0 1 8dJAakEiDUAwYqIyqGIVrjmEt2E the variant caused abnormal microtubule behavior in cell-end regions, which is likely to be the cause of the previously reported shape abnormalities ------- COMMENT: 324cb9a522d25d63 1 40KddBKxMMqVUz33G9CbUc8nEBM (comment: binds at Ter3 site) ------- COMMENT: 324cb9a522d25d63 4 fQW3oeaciWNjegJ2AhzioKhGb3s (comment: arrest at Ter2 and Ter3 sites abolished) ------- COMMENT: 324cb9a522d25d63 16 fQW3oeaciWNjegJ2AhzioKhGb3s (comment: arrest at Ter2 and Ter3 sites abolished) ------- COMMENT: 324cb9a522d25d63 18 fQW3oeaciWNjegJ2AhzioKhGb3s (comment: arrest at Ter2 and Ter3 sites abolished) ------- COMMENT: 324cb9a522d25d63 20 fQW3oeaciWNjegJ2AhzioKhGb3s (comment: arrest at Ter2 and Ter3 sites abolished) ------- COMMENT: 324cb9a522d25d63 22 fQW3oeaciWNjegJ2AhzioKhGb3s (comment: arrest at Ter2 and Ter3 sites abolished) ------- COMMENT: 324cb9a522d25d63 24 fQW3oeaciWNjegJ2AhzioKhGb3s (comment: arrest at Ter2 and Ter3 sites abolished) ------- COMMENT: 3309874a19179607 1 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 3309874a19179607 2 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 3309874a19179607 3 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 3309874a19179607 4 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 3309874a19179607 5 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 3309874a19179607 6 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 330f117d21a8480a 1 z8BsMpl9c9pmS0c4gaP043+Sh5w (comment: required for ubiquitination of Slp1) ------- COMMENT: 332ec190c658db98 10 8Vd933vcQsG5s50ilxJCd6w8uWE (comment: highest overexpression level) ------- COMMENT: 333ca30ce9c2eb22 16 iitAbqJyQjbyEMgo2sX0MY6o9hY (comment: WT 0.5%) ------- COMMENT: 33497d588756c658 9 JldTF7Vy5XM0u9fVMrtznY8FFcQ (comment: pol II CTD; probably S2 but can't rule out effect on S7) ------- COMMENT: 33497d588756c658 10 K6sfCAsroa7oidTfsJ0JEBL0MSQ (comment: polII CTD; probably S5 but can't rule out effect on S7) ------- COMMENT: 33497d588756c658 57 fAs4wDEYbvVEbSOr/ZYiQtkRbgI (comment: CHECK at act1 & sam1) ------- COMMENT: 33497d588756c658 58 fAs4wDEYbvVEbSOr/ZYiQtkRbgI (comment: CHECK at act1 & sam1) ------- COMMENT: 33497d588756c658 59 E2ARjVhhO1sU9vwSWkJxiNLDLbQ (comment: CHECK at ste11) ------- COMMENT: 33497d588756c658 60 E2ARjVhhO1sU9vwSWkJxiNLDLbQ (comment: CHECK at ste11) ------- COMMENT: 33497d588756c658 64 E2ARjVhhO1sU9vwSWkJxiNLDLbQ (comment: CHECK at ste11) ------- COMMENT: 33497d588756c658 68 E2ARjVhhO1sU9vwSWkJxiNLDLbQ (comment: CHECK at ste11) ------- COMMENT: 335eb22b9fc8a7c1 2 kFxTKIIEF3aP3Rk7ShFaLMHeEWA fig 1A ------- COMMENT: 335eb22b9fc8a7c1 3 kFxTKIIEF3aP3Rk7ShFaLMHeEWA fig 1A ------- COMMENT: 335eb22b9fc8a7c1 4 kFxTKIIEF3aP3Rk7ShFaLMHeEWA fig 1A ------- COMMENT: 335eb22b9fc8a7c1 5 kFxTKIIEF3aP3Rk7ShFaLMHeEWA fig 1A ------- COMMENT: 335eb22b9fc8a7c1 6 kFxTKIIEF3aP3Rk7ShFaLMHeEWA fig 1A ------- COMMENT: 335eb22b9fc8a7c1 7 kFxTKIIEF3aP3Rk7ShFaLMHeEWA fig 1A ------- COMMENT: 335eb22b9fc8a7c1 8 kFxTKIIEF3aP3Rk7ShFaLMHeEWA fig 1A ------- COMMENT: 335eb22b9fc8a7c1 9 kFxTKIIEF3aP3Rk7ShFaLMHeEWA fig 1A ------- COMMENT: 335eb22b9fc8a7c1 10 kFxTKIIEF3aP3Rk7ShFaLMHeEWA fig 1A ------- COMMENT: 335eb22b9fc8a7c1 11 kFxTKIIEF3aP3Rk7ShFaLMHeEWA fig 1A ------- COMMENT: 335eb22b9fc8a7c1 12 kFxTKIIEF3aP3Rk7ShFaLMHeEWA fig 1A ------- COMMENT: 335eb22b9fc8a7c1 13 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: 335eb22b9fc8a7c1 14 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: 335eb22b9fc8a7c1 15 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: 335eb22b9fc8a7c1 16 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: 335eb22b9fc8a7c1 17 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: 335eb22b9fc8a7c1 18 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: 335eb22b9fc8a7c1 19 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: 335eb22b9fc8a7c1 20 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: 335eb22b9fc8a7c1 21 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: 335eb22b9fc8a7c1 22 ksPmxkd4OqbBHzueaq6hxwt/QnQ fig1C ------- COMMENT: 335eb22b9fc8a7c1 23 ksPmxkd4OqbBHzueaq6hxwt/QnQ fig1C ------- COMMENT: 335eb22b9fc8a7c1 24 4OcmwDvrXHcQzBIq5V5Y7Rnix20 fig1C,D ------- COMMENT: 335eb22b9fc8a7c1 25 ksPmxkd4OqbBHzueaq6hxwt/QnQ fig1C ------- COMMENT: 335eb22b9fc8a7c1 26 ksPmxkd4OqbBHzueaq6hxwt/QnQ fig1C ------- COMMENT: 335eb22b9fc8a7c1 27 ksPmxkd4OqbBHzueaq6hxwt/QnQ fig1C ------- COMMENT: 335eb22b9fc8a7c1 28 ksPmxkd4OqbBHzueaq6hxwt/QnQ fig1C ------- COMMENT: 335eb22b9fc8a7c1 29 ksPmxkd4OqbBHzueaq6hxwt/QnQ fig1C ------- COMMENT: 335eb22b9fc8a7c1 31 RsdBr2g6Li7NFgcnnDaZXk0X+T8 fig1D ------- COMMENT: 335eb22b9fc8a7c1 32 RsdBr2g6Li7NFgcnnDaZXk0X+T8 fig1D ------- COMMENT: 335eb22b9fc8a7c1 33 RsdBr2g6Li7NFgcnnDaZXk0X+T8 fig1D ------- COMMENT: 335eb22b9fc8a7c1 34 ksPmxkd4OqbBHzueaq6hxwt/QnQ fig1C ------- COMMENT: 335eb22b9fc8a7c1 35 ksPmxkd4OqbBHzueaq6hxwt/QnQ fig1C ------- COMMENT: 335eb22b9fc8a7c1 36 U7VpfC4ENKJ+XBRES4OwbD2IAq4 Fig 2A III ------- COMMENT: 335eb22b9fc8a7c1 37 U7VpfC4ENKJ+XBRES4OwbD2IAq4 Fig 2A III ------- COMMENT: 335eb22b9fc8a7c1 38 U7VpfC4ENKJ+XBRES4OwbD2IAq4 Fig 2A III ------- COMMENT: 335eb22b9fc8a7c1 39 U7VpfC4ENKJ+XBRES4OwbD2IAq4 Fig 2A III ------- COMMENT: 335eb22b9fc8a7c1 40 MRv4U0wptZfY8tXQDHMhzMNic0c figure 2B ------- COMMENT: 335eb22b9fc8a7c1 41 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: 335eb22b9fc8a7c1 42 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: 335eb22b9fc8a7c1 43 kFxTKIIEF3aP3Rk7ShFaLMHeEWA fig1A ------- COMMENT: 335eb22b9fc8a7c1 44 kFxTKIIEF3aP3Rk7ShFaLMHeEWA fig1A ------- COMMENT: 338dbe24db5aa2ec 3 gC+OPtCC43GyDCtaG9nh6Kn4Rmc Figure S1C ------- COMMENT: 338dbe24db5aa2ec 4 gC+OPtCC43GyDCtaG9nh6Kn4Rmc Figure S1C ------- COMMENT: 338dbe24db5aa2ec 5 NWQ0FMKyS3MZhZESZmyzW8Mzi7s Figure S1E ------- COMMENT: 338dbe24db5aa2ec 6 NWQ0FMKyS3MZhZESZmyzW8Mzi7s Figure S1E ------- COMMENT: 338df954e1fab0ed 29 WWS9g5xPUCfz6M5lrQyePueKi/Y (comment: also assayed using Pil1 co-tethering with microscopy) ------- COMMENT: 338df954e1fab0ed 30 WWS9g5xPUCfz6M5lrQyePueKi/Y (comment: also assayed using Pil1 co-tethering with microscopy) ------- COMMENT: 33ab85af0ca986c2 9 hkyxEmH1yFXPqMaikCxH6QTdLm8 fig 9 ------- COMMENT: 33ab85af0ca986c2 10 hkyxEmH1yFXPqMaikCxH6QTdLm8 fig 9 ------- COMMENT: 33c4c7d6daa555cf 1 BhIBd8BRJ6BvgdruXN74HhT6uAQ Fig2a (comment: vw: average survival ~ 7 cell cycles) ------- COMMENT: 33c4c7d6daa555cf 2 wj2VSE893AMBlEWR8+9Ey55om+8 Fig 2B ------- COMMENT: 33c4c7d6daa555cf 7 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 33c4c7d6daa555cf 8 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 33c4c7d6daa555cf 9 1L40BjBlRfqsKTL9+a+85ulAlj8 Fig S4C ------- COMMENT: 33c4c7d6daa555cf 10 1L40BjBlRfqsKTL9+a+85ulAlj8 Fig S4C ------- COMMENT: 33c4c7d6daa555cf 11 1L40BjBlRfqsKTL9+a+85ulAlj8 Fig S4C ------- COMMENT: 33c4c7d6daa555cf 12 1L40BjBlRfqsKTL9+a+85ulAlj8 Fig S4C ------- COMMENT: 33c4c7d6daa555cf 13 nnuWhrFU7jaQ2CsXT4A21I2oLbM fig 3a ------- COMMENT: 33c4c7d6daa555cf 21 wA2zDi2ljZyRdZxkVyg7LW8joIY Fig 5C ------- COMMENT: 33c4c7d6daa555cf 22 foLZvNwPomujfsi3oAbbxeywskY fig 6A ------- COMMENT: 33c4c7d6daa555cf 24 wA2zDi2ljZyRdZxkVyg7LW8joIY Fig 5C ------- COMMENT: 33c4c7d6daa555cf 30 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 33c4c7d6daa555cf 46 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 33c4c7d6daa555cf 47 beCHAJaHpwcohMtQRG1wK84MoJs Supp S1F ------- COMMENT: 33c4c7d6daa555cf 48 ErU42VSwc1/Yi0oJgBUFHmtE5k8 Supp S1G ------- COMMENT: 33c4c7d6daa555cf 49 EK3cJaIpApHaZfDj/il8fbVV8BY fig S2 ------- COMMENT: 33c4c7d6daa555cf 50 EK3cJaIpApHaZfDj/il8fbVV8BY fig S2 ------- COMMENT: 33c4c7d6daa555cf 51 qdis3qJ9mmHu+YM1dObtly+CHtI Fig S2C,E ------- COMMENT: 33c4c7d6daa555cf 52 jo4YGrqojeORrq0+sWqihoBfSnc Fig S2,E ------- COMMENT: 33c4c7d6daa555cf 54 zaPPwnt/33QFwOvzDMtrVGN2h18 Fig S3 DE ------- COMMENT: 33c4c7d6daa555cf 55 zaPPwnt/33QFwOvzDMtrVGN2h18 Fig S3 DE ------- COMMENT: 33c4c7d6daa555cf 56 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: 33c4c7d6daa555cf 57 FJrUrY/cNbL7O7xtjP6w5yuBdIM fig 2b ------- COMMENT: 33c4c7d6daa555cf 58 DM4PjA1ccd8enI2LImQsaY4F74E fig S4E ------- COMMENT: 33c4c7d6daa555cf 59 DM4PjA1ccd8enI2LImQsaY4F74E fig S4E ------- COMMENT: 33c4c7d6daa555cf 61 jo4YGrqojeORrq0+sWqihoBfSnc Fig S2,E ------- COMMENT: 33c4c7d6daa555cf 69 5Pc+GJjJVhHm1h5Cm22Rhx2qUks fig 5B ------- COMMENT: 33c4c7d6daa555cf 70 4yCVBSkl6bg2aTrDdCZ1ZS1USlw fig 5b ------- COMMENT: 33c4c7d6daa555cf 71 Q6wcglkTAwEznYIykNVEEtyuQoc Fig5D and Movie 5 ------- COMMENT: 33c4c7d6daa555cf 73 ZSoIdAAkba/kthzeLNn7KxqZ/Bw Fig. S6B ------- COMMENT: 33c4c7d6daa555cf 74 lPer2ncYxtkCY1bCl13x5ILsRG4 Figure 6E ------- COMMENT: 33c4c7d6daa555cf 75 KwbgXoubBhjFig4BiRIv7+L/miQ Fig 7E ------- COMMENT: 33c4c7d6daa555cf 76 KwbgXoubBhjFig4BiRIv7+L/miQ Fig 7E ------- COMMENT: 33c4c7d6daa555cf 77 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 33c4c7d6daa555cf 78 5Pc+GJjJVhHm1h5Cm22Rhx2qUks fig 5B ------- COMMENT: 33c4c7d6daa555cf 80 5Pc+GJjJVhHm1h5Cm22Rhx2qUks fig 5B ------- COMMENT: 33c4c7d6daa555cf 81 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: 33c9bd391789a775 1 Tp2xtbhApn3LqeqZHDtimVAXIXw ------- COMMENT: 33c9bd391789a775 2 RJSP2PqUMqzuBcidM9BySxAy8SQ (comment: Queuosine absent from tRNA when cells are supplied with queuosine nucleoside, but not when supplied with queuine nucleobase) ------- COMMENT: 33d5d91d38abcc47 7 HMcKILkS82BNRqUzIz4NMXUW3vQ (comment: present throughout cell cycle) ------- COMMENT: 33e1584505554874 32 bvS7vE+Fm9IfdKXuKxkdX0mJ6pw (comment: clp1 cytoplasmic localization not maintained during cytokinetic stress. cdc7 localization to SPB not maintained during cytokinetic stress) ------- COMMENT: 33e4f8032d381a85 4 gZNo/xWKvKsHWWGOazkQEqseH2U Fig. 1 ------- COMMENT: 33e4f8032d381a85 13 Fboew5zeCyylDMkh6MpuwSXnqpM our study suggested that Phi1 was a part of the cohesin loading machinery (Fig. 5) ------- COMMENT: 33e4f8032d381a85 22 Fboew5zeCyylDMkh6MpuwSXnqpM our study suggested that Phi1 was a part of the cohesin loading machinery (Fig. 5) ------- COMMENT: 33e4f8032d381a85 23 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: 33e4f8032d381a85 24 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: 33e4f8032d381a85 25 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 33e4f8032d381a85 26 AC7h7XjhxLrVmVbP02VqEDE7z5w (Fig. 2C and D) ------- COMMENT: 33e4f8032d381a85 27 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 33e4f8032d381a85 28 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 33e4f8032d381a85 29 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 33e4f8032d381a85 30 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 33e4f8032d381a85 31 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 33e4f8032d381a85 32 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 33e4f8032d381a85 34 5a7fvmcTov3dOR2/Er4Wu3j4EM0 (Fig. 3A and B) ------- COMMENT: 33e4f8032d381a85 35 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: 33e4f8032d381a85 36 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 33e4f8032d381a85 37 gmqogAnY3BQANKEbZmiyaveRfrg (Fig. 3E) ------- COMMENT: 33e4f8032d381a85 38 DiT/uI6VVQeEU8sEguQD9JrMXlA (Fig. 4H and I) ------- COMMENT: 33e4f8032d381a85 41 lzDpBhI+e1KR8hAG7s00IgdEh2I (Fig. 5A) ------- COMMENT: 33e4f8032d381a85 42 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: 33e4f8032d381a85 43 qgy2UtS49Rc8vkPrVMir2qiYd3M (Fig. 5F) ------- COMMENT: 33e4f8032d381a85 44 qgy2UtS49Rc8vkPrVMir2qiYd3M (Fig. 5F) ------- COMMENT: 33e4f8032d381a85 45 qgy2UtS49Rc8vkPrVMir2qiYd3M (Fig. 5F) ------- COMMENT: 33e4f8032d381a85 46 ThL22xKsESPFoc4Z6QL36A4zq1I (Fig. 5D and F) ------- COMMENT: 33e4f8032d381a85 47 XVHahgPXZsFOyduY4BP8CoHD6gY (Fig. 5E and F) ------- COMMENT: 33f9124906c1e55e 1 ch8xHxjxNq9KtMc3O2rwhsckyps (comment: CHECK inhibited_by CHEBI:29035) ------- COMMENT: 3418ae66d9f08c88 1 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 341a050d011a7bfa 29 LZGiCiD8rrCuYLUYm4EL34SBsdc Fig. 1b ------- COMMENT: 341a050d011a7bfa 42 jgPxh0S8rof4lSz1TG30wnuWFWk Fig. 4e ------- COMMENT: 3431227650f88754 1 TUl9fngQi9T2r1Ci1WAnQSqMOPg fig 1a ------- COMMENT: 3431227650f88754 2 TUl9fngQi9T2r1Ci1WAnQSqMOPg fig 1a ------- COMMENT: 3431227650f88754 3 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: 3431227650f88754 4 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: 3431227650f88754 5 RPdkqAedukJ0cWGJswoHwJ+rPAE fig. 3 ------- COMMENT: 3448b2e04419517c 8 5YvUc8HvtX+75svkW1+vATeIgX0 ------- COMMENT: 3456bd4243b2feac 3 buny/ZYlVZXQe4vE3v3haR65xCw As shown in Fig. 2A, Mrz1 expression was decreased during the stationary phase grown on YES. ------- COMMENT: 3456bd4243b2feac 4 7+hXVgW457A3ODNFEbfOetQWh9g (comment: proteasome inhibitor MG132) ------- COMMENT: 3456bd4243b2feac 5 XfbZ7DtfOnj/wtoQcvxCA5PMz3w As observed, most of Mrz1 was localized in the mitochondrial fraction (Fig. 1B). ------- COMMENT: 3456bd4243b2feac 6 9R9hhe6ZXa+Kl5y5unLN2mJEf5Q Assessment of Mrz1 levels in these strains (Fig. 3) showed that the levels of Mrz1 markedly increased in ubc13 deletion background and no others, apart from the expected increase in the addition of MG132 controls. ------- COMMENT: 3456bd4243b2feac 7 pwpj/UKqW4SX5MgeEu7FhOs/Fjw The growth of the mrz1 mutant was almost like WT strain in both glucose- and glycerol-containing media at 30 °C (Fig. 4A). ------- COMMENT: 345d6b0c8bf7e493 2 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 345d6b0c8bf7e493 3 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 345d6b0c8bf7e493 4 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 345d6b0c8bf7e493 5 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 345d6b0c8bf7e493 6 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 345d6b0c8bf7e493 7 krSNa73S9t2LuF+otHi/az3pilA Fig. 2H ------- COMMENT: 345d6b0c8bf7e493 13 KzYiKM98UDcG7dwqvpALQoUJW7c (comment: chimera expressed from the ura4 locus [@ura4] - kept because not assayed from fus1 locus) ------- COMMENT: 345d6b0c8bf7e493 17 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 345d6b0c8bf7e493 19 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 345d6b0c8bf7e493 20 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 345d6b0c8bf7e493 21 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 345d6b0c8bf7e493 23 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 345d6b0c8bf7e493 24 vkT8uMpiBxgGlmYXEA8V3+LXigM Fig. 4E ------- COMMENT: 345d6b0c8bf7e493 25 vkT8uMpiBxgGlmYXEA8V3+LXigM Fig. 4E ------- COMMENT: 345d6b0c8bf7e493 26 vkT8uMpiBxgGlmYXEA8V3+LXigM Fig. 4E ------- COMMENT: 345d6b0c8bf7e493 27 vkT8uMpiBxgGlmYXEA8V3+LXigM Fig. 4E ------- COMMENT: 345d6b0c8bf7e493 28 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 345d6b0c8bf7e493 29 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 345d6b0c8bf7e493 30 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 345d6b0c8bf7e493 31 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 345d6b0c8bf7e493 32 cLKKHHathQCT1JZTf0i7RHzTu8A Fig. 5F ------- COMMENT: 345d6b0c8bf7e493 33 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 345d6b0c8bf7e493 34 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 345d6b0c8bf7e493 35 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 345d6b0c8bf7e493 36 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 345d6b0c8bf7e493 37 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 345d6b0c8bf7e493 38 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 345d6b0c8bf7e493 39 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 345d6b0c8bf7e493 40 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 345d6b0c8bf7e493 41 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 345d6b0c8bf7e493 42 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 345d6b0c8bf7e493 43 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 345d6b0c8bf7e493 44 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 345d6b0c8bf7e493 45 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 345d6b0c8bf7e493 46 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 345d6b0c8bf7e493 47 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 34790a1dd257be4f 14 mzmeduysSbxDCvnkwZUiqqFeMxs (comment: dephosphorylation of Cdc2 Y15 by Cdc25 delayed in response to ionising radiation) ------- COMMENT: 34790a1dd257be4f 23 T5CV1f6jrmoIQeWuR2t6xbGYU+Q (comment: Activity inhibited in response to mitotic G2 DNA damage checkpoint) ------- COMMENT: 34790a1dd257be4f 25 HvupdJiRQZyzLM9qckJuMuFdvlk (comment: temperature permissive for wee1-50; un-irradiated) ------- COMMENT: 34790a1dd257be4f 26 9bPgy3T7KKCU7bUF3ZnLy60kA/o (comment: cdc2-Y15F suppresses cell cycle arrest of chk1+ overexpression) ------- COMMENT: 34790a1dd257be4f 29 ihTAJ6wnwHjDrxQHwjnpR4uFEWY (comment: temperature restrictive for wee1-50) ------- COMMENT: 348a4b2740f9819e 1 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: 348a4b2740f9819e 2 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: 348a4b2740f9819e 3 JDje8AanoR6DlB1CaOryw/c0f2o Fig. 2B,C ------- COMMENT: 348a4b2740f9819e 4 +42Uny/0rBbKRKFHyDSMHCUtWeM (comment: CHECK DURATION) Fig. 2B,C ------- COMMENT: 348a4b2740f9819e 5 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 348a4b2740f9819e 6 /BBaYUwsw74FNFfzV3bsVR2u1cw (Table 2).leu1 and his2 loc, reduced 12 fold ------- COMMENT: 348a4b2740f9819e 7 EOLGFPOgoA6LvcRYE8yQndleBv4 (Table 3) assayed using pairing of his2 loci ------- COMMENT: 348a4b2740f9819e 8 Z2EOYTW9rSHYFmXkdH9O1shVXfw ------- COMMENT: 348a4b2740f9819e 9 owoQMOffrXW5Zizu2tAmAcvkgAY Table 3 ------- COMMENT: 348a4b2740f9819e 10 owoQMOffrXW5Zizu2tAmAcvkgAY Table 3 ------- COMMENT: 348a4b2740f9819e 11 owoQMOffrXW5Zizu2tAmAcvkgAY Table 3 ------- COMMENT: 348a4b2740f9819e 13 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 348a4b2740f9819e 14 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 348a4b2740f9819e 16 1DSx+sDsm3xYDLtMfzxH/90R880 Fig. 3C ------- COMMENT: 348a4b2740f9819e 18 fsp+xt+YWKsV74a2ljI7pFGtKGk data not shown , phenocopies ssm4 &dhc1 ------- COMMENT: 348a4b2740f9819e 19 fsp+xt+YWKsV74a2ljI7pFGtKGk data not shown , phenocopies ssm4 & dhc1 ------- COMMENT: 348a4b2740f9819e 27 sw1tm8NmjYULkvLMRPFfuL1j6a0 (Fig. 6B,C) (comment: in meiotic cells, shmooing cells) ------- COMMENT: 348a4b2740f9819e 28 sw1tm8NmjYULkvLMRPFfuL1j6a0 (Fig. 6B,C). (comment: in meiotic cells, shmooing cells) ------- COMMENT: 348a4b2740f9819e 29 sw1tm8NmjYULkvLMRPFfuL1j6a0 (Fig. 6B,C). in meiotic cells, shmooing cells ------- COMMENT: 348a4b2740f9819e 30 sw1tm8NmjYULkvLMRPFfuL1j6a0 (Fig. 6B,C). (comment: in meiotic cells, shmooing cells) ------- COMMENT: 348a4b2740f9819e 31 ACDocaPfsiN9PS4LIWiRBssxNmw (Fig. 6A-C), (comment: CHECK during meiotic prophase, shmooing) ------- COMMENT: 348a4b2740f9819e 32 Ey9Ve1JrBcMshSrTR7WAMTapQFo (comment: CHECK meiosis) ------- COMMENT: 34b5610ee8bc464d 5 WPuFk/tnqUlL9dbMwdV1vvGxSl8 ------- COMMENT: 34d3b03da3308cde 1 P63SPiHPSOuEPw7tc/L+hNfOgok ------- COMMENT: 34d3b03da3308cde 2 m3WnuWPF46pk3a/lO7kDGGqwI8Q ------- COMMENT: 34f78a5c7e2cca72 7 M5+flN7EAeU/GB+L83OGb1NydGo (comment: elimination of Rad3-specific phosphorylation)| ------- COMMENT: 34f78a5c7e2cca72 8 PhYGQZkoEmdnZ9V1LA65MGoE3GA (comment: reduced chk1 phosphorylation) ------- COMMENT: 34f90a1facdd9e18 3 UKKmDFSl9brTZ4U7Wnec8LerQE8 Importantly,CK2-mediated phosphorylation had a similar effect on the nucleosome-binding specificities of fly HP1a and S.pombe Swi6. (Figure 6A) ------- COMMENT: 3520ae1d8f10669a 1 27eFIhItkW6RnyjrueTOSpKCpfA Figure 1A and B ------- COMMENT: 3520ae1d8f10669a 2 27eFIhItkW6RnyjrueTOSpKCpfA Figure 1A and B ------- COMMENT: 3520ae1d8f10669a 3 hqZEhB4OpPNtUpFSSp7m4a5jLD0 Spores deleted for etd1 (etd1D) germinated and accumulated multiple nuclei without septation, an identical phenotype to that of etd1-1 mutant cells under restrictive conditions (Figure 1C). ------- COMMENT: 3520ae1d8f10669a 4 3Kn71UpvLLgvo/ac/AJ7/K1qS7U Under derepressed conditions ( thiamine), Etd1p overproduction generated elongated and multinucleate cells in both etd1-1 mutant and wild-type backgrounds (Figure 1D and data not shown). Thus, the phenotypic defect caused by an excess of Etd1p was identical to that produced by a deficiency of this protein, suggesting that Etd1p functions in a stoichiometric protein complex. ------- COMMENT: 3520ae1d8f10669a 6 UfA19BEHovONK1X7u+AKDrPhUeg In interphase cells, Etd1p-GFP was located at the cell cortex and was more concentrated at the cell tips (Figure 2A, cell 1). ------- COMMENT: 3520ae1d8f10669a 7 AGP/RpeAwDhnzIZc54/KNEmSbTM In early anaphase, Etd1p-GFP became concentrated in the medial region of the cell cortex as a broad band (Figure 2A, cell 2) ------- COMMENT: 3520ae1d8f10669a 8 AGP/RpeAwDhnzIZc54/KNEmSbTM In early anaphase, Etd1p-GFP became concentrated in the medial region of the cell cortex as a broad band (Figure 2A, cell 2) ------- COMMENT: 3520ae1d8f10669a 9 HsqB1bC2Rij37KjbPL/L23uXCCs We therefore analysed the localisation of Etd1p-GFP in cdc8-110 mutant cells and found that, at the restrictive temperature of 361C, Etd1p never formed a ring (Figure 3B, upper panels). ------- COMMENT: 3520ae1d8f10669a 10 HsqB1bC2Rij37KjbPL/L23uXCCs We therefore analysed the localisation of Etd1p-GFP in cdc8-110 mutant cells and found that, at the restrictive temperature of 361C, Etd1p never formed a ring (Figure 3B, upper panels). ------- COMMENT: 3520ae1d8f10669a 11 sRUAv3JhzeYlxWxBfM0JVpiU95s Etd1p-GFP, demonstrating that Etd1p interacts physically with Cdc15p. Similarly, Cdc15p was detected in anti-GFP immune complexes (data not shown). Thus, Etd1p may localise to the actomyosin ring by association with Cdc15p. (comment: the anchor is using 2022 knowledge) ------- COMMENT: 3520ae1d8f10669a 12 wrF7pVweEZI0uo6Gq9RMbfrprI0 However, in etd1-1 mutant cells, the medial ring marked with Cdc15p-GFP seems to fail constriction. To bette ------- COMMENT: 3520ae1d8f10669a 16 cp/eKCd41+ORt+oj/EqrMdcZSIs Etd1p-GFP failed to localise to the medial ring at the restrictive temperature. Instead, these mutant cells accumulated Etd1-GFP in a broad band at the plasma membrane overlying the site of cytokinesis (Figure 6A, upper panel, ------- COMMENT: 3520ae1d8f10669a 17 Aubvp1sgNUUGi5/hE3jKIziU+ic (comment: missing annotation, we dont have that cdc7 is on old SPB in metaphase) Cdc7p-GFP appeared at both SPBs at the initiation of mitosis and only at one SPB as cells progressed through anaphase until the completion of cell division. ------- COMMENT: 3520ae1d8f10669a 18 Ow2GXJ6ypjBcxNDmoFQ/N0XneCQ suggesting that Etd1p is somehow necessary to maintain Spg1p activity during anaphase until the completion of cytokinesis ------- COMMENT: 3520ae1d8f10669a 20 kd5o4iQerQpY2qbMb8L+aPDMVvo These results indicate that Etd1p is polyubiquitinated and degraded through the ubiquitin-dependent 26S-proteasome pathway. ------- COMMENT: 352bbbd10b1d6ce0 8 DrhvN1TNj25+dD9ivIOI0Hl5Wuo ------- COMMENT: 352bbbd10b1d6ce0 15 87N4fNEjVi0c7oCyGPDRCtWMngU (comment: CHECK Increased MMS sensitivity) ------- COMMENT: 352bbbd10b1d6ce0 30 87N4fNEjVi0c7oCyGPDRCtWMngU (comment: CHECK Increased MMS sensitivity) ------- COMMENT: 358a49422d80513c 1 GFa9/7gcmHJn/Ut6T6+aPMsBDME Fig. S1 B ------- COMMENT: 358a49422d80513c 3 MNzgBF0ib4W/fVrcjV0pvZl2FAE Fig. S1 C ------- COMMENT: 358a49422d80513c 16 Bf+PXSW16D/f4m/FgrLXo2bwvC0 (comment: arrested normal size (multiple rounds of cytokinesis) in interphase) ------- COMMENT: 358a49422d80513c 19 kkB9Mih85yOrFZPwC5pb1xG2W98 Fig. 4B ------- COMMENT: 358a49422d80513c 20 c0uMiNqcE3BgpszPax9cHtCbVbg Fig. 4D ------- COMMENT: 358a49422d80513c 21 kkB9Mih85yOrFZPwC5pb1xG2W98 Fig. 4B ------- COMMENT: 358a49422d80513c 22 64PELSOLLbsOqBWZtv7zciI0u1c (comment: CHECK 2%) fig 6a ------- COMMENT: 358a49422d80513c 23 KxqnuJVo17tlmzQ/M+E9noNW6bQ (comment: CHECK 2%) fig 6a. (comment: to dauughter) ------- COMMENT: 358a49422d80513c 24 joVMR5uR3eJfqXkNDVQ/vfmwPQo (comment: CHECL add to def, septated in interphase. one compartment is anucleate) ------- COMMENT: 35a8810c7dfc8c9d 1 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 35a8810c7dfc8c9d 2 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 35a8810c7dfc8c9d 3 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 35a8810c7dfc8c9d 4 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 35a8810c7dfc8c9d 5 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 35a8810c7dfc8c9d 6 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 35a8810c7dfc8c9d 7 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 35a8810c7dfc8c9d 8 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 35a8810c7dfc8c9d 9 BifuiKTmX/nPd5H+Vjk05zkmDMU Table 2 and Fig. 4 ------- COMMENT: 35a8810c7dfc8c9d 10 BifuiKTmX/nPd5H+Vjk05zkmDMU Table 2 and Fig. 4 ------- COMMENT: 35a8810c7dfc8c9d 11 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 35a8810c7dfc8c9d 12 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 35a8810c7dfc8c9d 13 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 35a8810c7dfc8c9d 14 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 35a8810c7dfc8c9d 15 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 35a8810c7dfc8c9d 16 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 35a8810c7dfc8c9d 17 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 35a8810c7dfc8c9d 18 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 35a8810c7dfc8c9d 19 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 35a8810c7dfc8c9d 20 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 35a8810c7dfc8c9d 21 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 35a8810c7dfc8c9d 22 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: 35a8810c7dfc8c9d 23 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: 35a8810c7dfc8c9d 24 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: 35a8810c7dfc8c9d 25 bMOThth5RZ7UvMAO/LuAu0jDKa0 Fig. 8C ------- COMMENT: 35a8810c7dfc8c9d 26 bMOThth5RZ7UvMAO/LuAu0jDKa0 Fig. 8C ------- COMMENT: 35a8810c7dfc8c9d 27 bMOThth5RZ7UvMAO/LuAu0jDKa0 Fig. 8C ------- COMMENT: 35aab4b08bf43fb8 5 aYawOzcYocKtZrVyKBNdnPs9/Bk (comment: rec12-201::6His-2FLAG(C-terminal 6His-2FLAG tag)) ------- COMMENT: 35aab4b08bf43fb8 6 aYawOzcYocKtZrVyKBNdnPs9/Bk (comment: rec12-201::6His-2FLAG(C-terminal 6His-2FLAG tag)) ------- COMMENT: 35aab4b08bf43fb8 7 aYawOzcYocKtZrVyKBNdnPs9/Bk ((comment: rec12-201::6His-2FLAG(C-terminal 6His-2FLAG tag)) ------- COMMENT: 35aab4b08bf43fb8 8 aYawOzcYocKtZrVyKBNdnPs9/Bk (comment: ChIP-CHIP, rec12-201::6His-2FLAG(C-terminal 6His-2FLAG tag)) ------- COMMENT: 35aab4b08bf43fb8 9 aYawOzcYocKtZrVyKBNdnPs9/Bk (comment: ChIP-CHIP, rec12-201::6His-2FLAG(C-terminal 6His-2FLAG tag)) ------- COMMENT: 35aab4b08bf43fb8 10 Aj9tepGNto8dhZoPqPkWnhttDsA (comment: ChIP-CHIP, rec27-205::GFP-kanMX6(C-terminal GFP tag)) ------- COMMENT: 35aab4b08bf43fb8 11 BkhM0DeALfPKYYI9HlJVwEfHVPM (comment: mug20::GFP-kanMX6(C-terminal GFP tag)) ------- COMMENT: 35aab4b08bf43fb8 12 BkhM0DeALfPKYYI9HlJVwEfHVPM (comment: mug20::GFP-kanMX6(C-terminal GFP tag)) ------- COMMENT: 35aab4b08bf43fb8 13 BkhM0DeALfPKYYI9HlJVwEfHVPM (comment: mug20::GFP-kanMX6(C-terminal GFP tag)) ------- COMMENT: 35aab4b08bf43fb8 14 BkhM0DeALfPKYYI9HlJVwEfHVPM (comment: mug20::GFP-kanMX6(C-terminal GFP tag)) ------- COMMENT: 35aab4b08bf43fb8 15 aCu36z5Z7XAIuhCwU/FMasYbKBM (comment: rec25-204::GFP-kanMX6(C-terminal GFP tag)) ------- COMMENT: 35aab4b08bf43fb8 16 Aj9tepGNto8dhZoPqPkWnhttDsA (comment: rec27-205::GFP-kanMX6(C-terminal GFP tag)) ------- COMMENT: 35aab4b08bf43fb8 24 BkhM0DeALfPKYYI9HlJVwEfHVPM (comment: mug20::GFP-kanMX6(C-terminal GFP tag)) ------- COMMENT: 35aab4b08bf43fb8 25 BkhM0DeALfPKYYI9HlJVwEfHVPM (comment: mug20::GFP-kanMX6(C-terminal GFP tag)) ------- COMMENT: 35aab4b08bf43fb8 26 BkhM0DeALfPKYYI9HlJVwEfHVPM (comment: mug20::GFP-kanMX6(C-terminal GFP tag)) ------- COMMENT: 35aab4b08bf43fb8 27 BkhM0DeALfPKYYI9HlJVwEfHVPM (comment: mug20::GFP-kanMX6(C-terminal GFP tag)) ------- COMMENT: 35aab4b08bf43fb8 28 aCu36z5Z7XAIuhCwU/FMasYbKBM (comment: rec25-204::GFP-kanMX6(C-terminal GFP tag)) ------- COMMENT: 35aab4b08bf43fb8 29 7gIffQNC6cs6kSrmeJ84bpe9VZw (comment: rec27-205::GFP-kanMX6(C-terminal GFP tag)(comment: ------- COMMENT: 35c498b7a5ea246c 4 G/ehcYFtH9qf9ywshcI5/Mqib/k (comment: assayed substrate: rabbit muscle phosphorylase) ------- COMMENT: 35ce1fe63f12db9a 1 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: 35ce1fe63f12db9a 2 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: 35ce1fe63f12db9a 3 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: 35d761b3ba38e2ac 9 WQ+lN6YPDvB7qf8G6YALgYPm30w In contrast to the point mutation, the rec8::ura4 strain showed no shortening of prophase in three independent time courses (data not shown). Shortening of the prophase in the point mutation strain may indicate a role of Rec8p in meiosis regulation. Alternative explanations, like shortening of prophase by an additional mutation, were not excluded. ------- COMMENT: 35ddc02d3b26fd99 12 GClfETmhVHLg/BerzCuT7d1KL74 (comment: same as rad3delta alone) ------- COMMENT: 35ddc02d3b26fd99 13 zUKmKhZc1WWjM/4LCry/+ZYpP+I (comment: same as rad26delta alone) ------- COMMENT: 35ddc02d3b26fd99 16 v7aVk9xNS+N/el2RIbSwp0P/AZ0 (comment: same as crb2delta alone) ------- COMMENT: 35e6ffe290c8f5c3 3 n56+QiB978HMtDCMbFaQ7p5D3cE FLAG ChIP analysis revealed that H297A reduced appreciably Epe1 enrichment on centromeric dg repeats and IRC3 (Fig 4G), a centro- meric boundary sequence where Epe1 accumulates to a high level [12, 14 ------- COMMENT: 35e6ffe290c8f5c3 8 /a4+vLOfaeJd6u6sYw66s+aBNVo The H297A substitution slightly impaired the interaction (S4G Fig), which was confirmed by the results of a bait-prey exchange experiment. We performed co-immunoprecipitation analysis of Swi6 with Epe1H297A. Con- sistent with the results of yeast two-hybrid assay, the Epe1H297A mutant interacted with Swi6 with a slightly lower efficiency than wild-type Epe1 (Fig 4H). ------- COMMENT: 35e6ffe290c8f5c3 9 8PsQsPxIhtSCXPBHwWqSsGIN0hY (comment: vw: variegated population) These results suggested that the white phenotype of W70 was not linked to otr1R::ade6+. The re-appearance of red colonies from epe1Δ W70 cells (Fig 1C) suggested that the white phenotype was due to epigenetic rather than genetic alterations. ------- COMMENT: 35e6ffe290c8f5c3 10 F3oMRFWo5bxNwqhBqzehNcbHXzY Since loss of Epe1 increases H3K9me levels at subtel1L and 2L [23, 24], we hypothesized that the ade5 gene was silenced by ectopi- cally deposited H3K9me, which arrested red pigment formation. ------- COMMENT: 35e6ffe290c8f5c3 11 90x4clc38I6LLqeU1SLbqgSc5Lo Thus, we concluded that ectopic heterochromatin-mediated repression of ade5 caused the white phenotype of the epe1Δ W70 strain. ------- COMMENT: 35e6ffe290c8f5c3 12 SLVFB4BzMRfeXPuJ11ofb9yCkIg Loss of Clr4 abolished red-white variegation in the epe1Δ ade6-m210 background, indicating a requirement for the H3K9 methyltransferase Clr4. clr3 and sir2, which encode his- tone deacetylases, are required for self-propagation of heterochromatin [30–34]. The introduc- tion of clr3Δ or sir2Δ into the epe1Δ background also induced a uniform red phenotype. Similarly, loss of Swi6 suppressed variegation. ------- COMMENT: 35e6ffe290c8f5c3 13 SLVFB4BzMRfeXPuJ11ofb9yCkIg Loss of Clr4 abolished red-white variegation in the epe1Δ ade6-m210 background, indicating a requirement for the H3K9 methyltransferase Clr4. clr3 and sir2, which encode his- tone deacetylases, are required for self-propagation of heterochromatin [30–34]. The introduc- tion of clr3Δ or sir2Δ into the epe1Δ background also induced a uniform red phenotype. Similarly, loss of Swi6 suppressed variegation. ------- COMMENT: 35e6ffe290c8f5c3 14 SLVFB4BzMRfeXPuJ11ofb9yCkIg Loss of Clr4 abolished red-white variegation in the epe1Δ ade6-m210 background, indicating a requirement for the H3K9 methyltransferase Clr4. clr3 and sir2, which encode his- tone deacetylases, are required for self-propagation of heterochromatin [30–34]. The introduc- tion of clr3Δ or sir2Δ into the epe1Δ background also induced a uniform red phenotype. Similarly, loss of Swi6 suppressed variegation. ------- COMMENT: 35e6ffe290c8f5c3 15 SLVFB4BzMRfeXPuJ11ofb9yCkIg Loss of Clr4 abolished red-white variegation in the epe1Δ ade6-m210 background, indicating a requirement for the H3K9 methyltransferase Clr4. clr3 and sir2, which encode his- tone deacetylases, are required for self-propagation of heterochromatin [30–34]. The introduc- tion of clr3Δ or sir2Δ into the epe1Δ background also induced a uniform red phenotype. Similarly, loss of Swi6 suppressed variegation. ------- COMMENT: 35e6ffe290c8f5c3 16 bF6O7nId+EfIio5eqKZdbksVzhM We next examined the requirement for Ago1 and Taz1 for epe1Δ-induced variegation, because both factors are involved in subtelomeric constitutive heterochromatin formation [5]. epe1Δ ago1Δ, epe1Δ taz1Δ, and epe1Δ ago1Δ taz1Δ strains displayed red-white variegated phe- notypes (S2A and S2B Fig), indicating that neither RNAi nor Taz1 was essential for epe1Δ- induced variegation. ------- COMMENT: 35e6ffe290c8f5c3 17 bF6O7nId+EfIio5eqKZdbksVzhM We next examined the requirement for Ago1 and Taz1 for epe1Δ-induced variegation, because both factors are involved in subtelomeric constitutive heterochromatin formation [5]. epe1Δ ago1Δ, epe1Δ taz1Δ, and epe1Δ ago1Δ taz1Δ strains displayed red-white variegated phe- notypes (S2A and S2B Fig), indicating that neither RNAi nor Taz1 was essential for epe1Δ- induced variegation. ------- COMMENT: 35e6ffe290c8f5c3 18 bF6O7nId+EfIio5eqKZdbksVzhM We next examined the requirement for Ago1 and Taz1 for epe1Δ-induced variegation, because both factors are involved in subtelomeric constitutive heterochromatin formation [5]. epe1Δ ago1Δ, epe1Δ taz1Δ, and epe1Δ ago1Δ taz1Δ strains displayed red-white variegated phe- notypes (S2A and S2B Fig), indicating that neither RNAi nor Taz1 was essential for epe1Δ- induced variegation. ------- COMMENT: 35e6ffe290c8f5c3 19 KouMZ6AelAjIM+lnMuC0R73uVBs Unlike loss of Epe1, the H297A mutation generated few pink/white colonies in the ade6-m210 background (Fig 4A); indeed, 96.2% of Epe1H297A cells formed WT-like red colonies, while 61.7% of epe1Δ cells did. ------- COMMENT: 35e6ffe290c8f5c3 20 sICipJydKsno+kwdqyGXtznwBss However, how Epe1 finds target sites to prevent ectopic heterochromatin formation is unknown. Since Epe1 physically interacts with the bromodomain protein Bdf2, which is required for heterochromatin-euchromatin boundary formation [14], we predicted that Bdf2 would recruit Epe1 to the target sites. However, bdf2Δ cells showed an almost uniform red phenotype in the ade6-m210 background (S4H Fig), suggesting that Bdf2 was not related to suppression of variegation and ectopic heterochromatin formation. ------- COMMENT: 35e6ffe290c8f5c3 21 H7sLGq12gKoBEi0/m/OEVRz2mdw We found that deletion of the N-terminal 171 amino acids (Epe1ΔN) abolished transcriptional activation by Epe1 and the N-terminal 208 amino acids (Epe1N208) activated transcription of the HIS3 reporter independently of JmjC (Fig 4I), suggesting that the N-terminal 171 amino acids region is required for the transcriptional activation activity. ------- COMMENT: 35e6ffe290c8f5c3 22 EJHS9BSj2lkxJk1BNzamYe8ulTI We introduced Epe1ΔN into ade6-m210 cells to examine the effect of the ΔN mutation on the suppression of ectopic heterochromatin formation. Epe1ΔN cells formed pink/white colonies with a slightly lower frequency than epe1Δ cells (Fig 4K), indicating that the NTA domain contributed to the suppression of ectopic heterochromatin-mediated variegation. ------- COMMENT: 35e6ffe290c8f5c3 23 Fv9zi4wJHjyoBHffV3qeMNyQnL0 However, consistent with the previous report [13], the C-terminal half of Epe1 (487–948 amino acids region) interacted with Swi6 in the yeast two-hybrid system, but the N-terminal half (1–486) did not (S4I Fig). ------- COMMENT: 35e6ffe290c8f5c3 24 Fv9zi4wJHjyoBHffV3qeMNyQnL0 However, consistent with the previous report [13], the C-terminal half of Epe1 (487–948 amino acids region) interacted with Swi6 in the yeast two-hybrid system, but the N-terminal half (1–486) did not (S4I Fig). ------- COMMENT: 3602d714a22a5fa7 15 3kkgGv8nY2M5kiDU2fi8lxeWUrk (comment: CHECK- add background? G1 arrested cells) ------- COMMENT: 3602d714a22a5fa7 16 FgRo6MOIxWi1MQPzsXFV1FX6Q9U (comment: CHECK add background G1 arrested cells) ------- COMMENT: 36502c2f15fe4801 1 C+yjTbwSKI9vWYTKIOcq3hbQKsM (Figure 4 B) S. pombe tan1Δ mutants are temperature sensitive due to decay of two tRNA species by the RTD pathway. ------- COMMENT: 36502c2f15fe4801 2 naEMYYn0v0X7nf22zpx4dei6kn4 (Fig. 1A) ------- COMMENT: 36502c2f15fe4801 4 naEMYYn0v0X7nf22zpx4dei6kn4 (Fig. 1A) ------- COMMENT: 36502c2f15fe4801 5 naEMYYn0v0X7nf22zpx4dei6kn4 (Fig. 1A) ------- COMMENT: 36502c2f15fe4801 6 VxRRkeXnzgPBe7/2oM/Q/abKEQM (comment: vw: grows slightly better than WT) However, unlike the dhp1 suppres- sors, these suppressors grew poorly on media containing 5-FU at 33 ̊C, and unlike the GAAC or dhp1 suppressors, these suppressors were resistant to 3-AT at 37 ̊C (Figs 1A and S1) ------- COMMENT: 36502c2f15fe4801 7 naEMYYn0v0X7nf22zpx4dei6kn4 (Fig. 1A) ------- COMMENT: 36502c2f15fe4801 8 U/MWLkT88V1iGct+r2ZdgiDKcQE (Fig. 1A) In this way, we identified a group of four trm8Δ suppressors that suppressed trm8Δ temper- ature sensitivity in rich (YES) and complete minimal media lacking histidine (EMMC-His) almost as efficiently as a trm8Δ suppressor with a representative dhp1 mutation (trm8Δ dhp1-W326L) or GAAC mutation (trm8Δ gcn2-M1I) ------- COMMENT: 36502c2f15fe4801 9 VxRRkeXnzgPBe7/2oM/Q/abKEQM (comment: vw: grows slightly better than WT) However, unlike the dhp1 suppres- sors, these suppressors grew poorly on media containing 5-FU at 33 ̊C, and unlike the GAAC or dhp1 suppressors, these suppressors were resistant to 3-AT at 37 ̊C (Figs 1A and S1) ------- COMMENT: 36502c2f15fe4801 10 naEMYYn0v0X7nf22zpx4dei6kn4 (Fig. 1A) ------- COMMENT: 36502c2f15fe4801 11 naEMYYn0v0X7nf22zpx4dei6kn4 (Fig. 1A) ------- COMMENT: 36502c2f15fe4801 12 U/MWLkT88V1iGct+r2ZdgiDKcQE (Fig. 1A) In this way, we identified a group of four trm8Δ suppressors that suppressed trm8Δ temper- ature sensitivity in rich (YES) and complete minimal media lacking histidine (EMMC-His) almost as efficiently as a trm8Δ suppressor with a representative dhp1 mutation (trm8Δ dhp1-W326L) or GAAC mutation (trm8Δ gcn2-M1I) ------- COMMENT: 36502c2f15fe4801 15 oulQxtX6VKcNPrpNcXEnzVYJhqM (Fig. 1A) In this way, we identified a group of four trm8Δ suppressors that suppressed trm8Δ temper- ature sensitivity in rich (YES) and complete minimal media lacking histidine (EMMC-His) almost as efficiently as a trm8Δ suppressor with a representative dhp1 mutation (trm8Δ dhp1-W326L) or GAAC mutation (trm8Δ gcn2-M1I) [23 ------- COMMENT: 36502c2f15fe4801 18 oulQxtX6VKcNPrpNcXEnzVYJhqM (Fig. 1A) In this way, we identified a group of four trm8Δ suppressors that suppressed trm8Δ temper- ature sensitivity in rich (YES) and complete minimal media lacking histidine (EMMC-His) almost as efficiently as a trm8Δ suppressor with a representative dhp1 mutation (trm8Δ dhp1-W326L) or GAAC mutation (trm8Δ gcn2-M1I) [23 ------- COMMENT: 36502c2f15fe4801 19 oulQxtX6VKcNPrpNcXEnzVYJhqM (Fig. 1A) In this way, we identified a group of four trm8Δ suppressors that suppressed trm8Δ temper- ature sensitivity in rich (YES) and complete minimal media lacking histidine (EMMC-His) almost as efficiently as a trm8Δ suppressor with a representative dhp1 mutation (trm8Δ dhp1-W326L) or GAAC mutation (trm8Δ gcn2-M1I) [23 ------- COMMENT: 36502c2f15fe4801 20 U/MWLkT88V1iGct+r2ZdgiDKcQE (Fig. 1A) In this way, we identified a group of four trm8Δ suppressors that suppressed trm8Δ temper- ature sensitivity in rich (YES) and complete minimal media lacking histidine (EMMC-His) almost as efficiently as a trm8Δ suppressor with a representative dhp1 mutation (trm8Δ dhp1-W326L) or GAAC mutation (trm8Δ gcn2-M1I) ------- COMMENT: 36502c2f15fe4801 21 U/MWLkT88V1iGct+r2ZdgiDKcQE (Fig. 1A) In this way, we identified a group of four trm8Δ suppressors that suppressed trm8Δ temper- ature sensitivity in rich (YES) and complete minimal media lacking histidine (EMMC-His) almost as efficiently as a trm8Δ suppressor with a representative dhp1 mutation (trm8Δ dhp1-W326L) or GAAC mutation (trm8Δ gcn2-M1I) ------- COMMENT: 36502c2f15fe4801 22 U/MWLkT88V1iGct+r2ZdgiDKcQE (Fig. 1A) In this way, we identified a group of four trm8Δ suppressors that suppressed trm8Δ temper- ature sensitivity in rich (YES) and complete minimal media lacking histidine (EMMC-His) almost as efficiently as a trm8Δ suppressor with a representative dhp1 mutation (trm8Δ dhp1-W326L) or GAAC mutation (trm8Δ gcn2-M1I) ------- COMMENT: 36502c2f15fe4801 23 w2ynNy8P84C+ur/JxVz1IrJULec However, unlike the dhp1 suppressors, these suppressors grew poorly on media containing 5-FU at 33 ̊C (Fig 1A) ------- COMMENT: 36502c2f15fe4801 24 w2ynNy8P84C+ur/JxVz1IrJULec However, unlike the dhp1 suppressors, these suppressors grew poorly on media containing 5-FU at 33 ̊C (Fig 1A) ------- COMMENT: 36502c2f15fe4801 25 w2ynNy8P84C+ur/JxVz1IrJULec However, unlike the dhp1 suppressors, these suppressors grew poorly on media containing 5-FU at 33 ̊C (Fig 1A) ------- COMMENT: 36502c2f15fe4801 26 JAPBqrT+430vCf7x6Yeja55RExc and unlike the GAAC or dhp1 suppressors, these suppressors were resistant to 3-AT at 37 ̊C (Fig 1A) ------- COMMENT: 36502c2f15fe4801 27 JAPBqrT+430vCf7x6Yeja55RExc and unlike the GAAC or dhp1 suppressors, these suppressors were resistant to 3-AT at 37 ̊C (Fig 1A) ------- COMMENT: 36502c2f15fe4801 28 JAPBqrT+430vCf7x6Yeja55RExc and unlike the GAAC or dhp1 suppressors, these suppressors were resistant to 3-AT at 37 ̊C (Fig 1A) ------- COMMENT: 36502c2f15fe4801 29 oulQxtX6VKcNPrpNcXEnzVYJhqM (Fig. 1A) In this way, we identified a group of four trm8Δ suppressors that suppressed trm8Δ temper- ature sensitivity in rich (YES) and complete minimal media lacking histidine (EMMC-His) almost as efficiently as a trm8Δ suppressor with a representative dhp1 mutation (trm8Δ dhp1-W326L) or GAAC mutation (trm8Δ gcn2-M1I) [23 ------- COMMENT: 36502c2f15fe4801 30 mZIaBSeRl7zkMnJX7FkmgOLg8dU (Figs 1A and S1) In this way, we identified a group of four trm8Δ suppressors that suppressed trm8Δ temper- ature sensitivity in rich (YES) and complete minimal media lacking histidine (EMMC-His) almost as efficiently as a trm8Δ suppressor with a representative dhp1 mutation (trm8Δ dhp1-W326L) or GAAC mutation (trm8Δ gcn2-M1I) ------- COMMENT: 36502c2f15fe4801 31 GQCAxaNaghyMDIA6CssgvRWNZWs (Figs 1A and S1) However, unlike the dhp1 suppressors, these suppressors grew poorly on media containing 5-FU at 33 ̊C (Figs 1A) ------- COMMENT: 36502c2f15fe4801 32 vqadOHnARxguZooNJkg31Y7RHlE (Figs 1A and S1) and unlike the GAAC or dhp1 suppressors, these suppressors were resistant to 3-AT at 37 ̊C ------- COMMENT: 36502c2f15fe4801 33 /PvmilCdYlobtlpcjL5HR4RzFxE As expected, in trm8Δ mutants at 38.5 ̊C, relative tRNATyr(GUA) levels were reduced, to 30% of WT levels (Fig 1B and 1C). ------- COMMENT: 36502c2f15fe4801 34 01xINPK4pf/KqAZGm/Ki8vjYGpI Surprisingly, we found that in the trm8Δ rpl1701-Q72X suppressor the tRNATyr(GUA) levels were not restored, but were if anything slightly reduced, relative to the trm8Δ mutant (23% vs 30%).....Thus, for the trm8Δ rpl1701-Q72X suppressor, suppres- sion is occurring without a detectable increase in levels of tRNATyr(GUA) or of tRNAPro(AGG). ------- COMMENT: 36502c2f15fe4801 35 EVH6mt5mDVJIOKeeltSOb8PApkQ Similar analysis shows that the trm8Δ rpl502-Y44X suppressor had slightly increased levels of tRNATyr(GUA) (36% vs 30%) and of tRNAPro(AGG) (13% vs 9%) relative to WT (Fig 1B and 1C), ------- COMMENT: 36502c2f15fe4801 36 +QmdoLe/F3ZalquA3+H7WNIZU+k and a follow-up analysis shows that the trm8Δ rpl1102-K93X suppressor also did not restore the reduced levels of tRNATyr(GUA) and tRNAPro(AGG), whereas levels of a known unaf- fected Trm8 substrate (tRNAVal(AAC)) remained constant (S3 Fig). ------- COMMENT: 36502c2f15fe4801 38 qjsA1O0+AVcd+3mn/e0dlGMpe7M By contrast, trm8Δ rpl1701-Q72X and trm8Δ rpl502-Y44X mutants had near baseline relative lys4+ expression at both tempera- tures (0.97 and 0.88 vs 0.88 for WT, at 38.5 ̊C) (Fig 1D). ------- COMMENT: 36502c2f15fe4801 40 qjsA1O0+AVcd+3mn/e0dlGMpe7M By contrast, trm8Δ rpl1701-Q72X and trm8Δ rpl502-Y44X mutants had near baseline relative lys4+ expression at both tempera- tures (0.97 and 0.88 vs 0.88 for WT, at 38.5 ̊C) (Fig 1D). ------- COMMENT: 36502c2f15fe4801 41 aO+E8SILr3iStpwJ4sEVU63lw7g We found that a reconstructed trm8Δ rpl502Δ strain suppressed the growth defect of a trm8Δ mutant almost identically to the trm8Δ rpl502-Y44X mutant, with similar growth in YES and EMMC media at high temperature, and similar 5-FU sensitivity and 3-AT resistance (Fig 2A) ------- COMMENT: 36502c2f15fe4801 42 Y7DGZXbX4TbUg4hpPkwWQ0vFKJg We found that a reconstructed trm8Δ rpl502Δ strain suppressed the growth defect of a trm8Δ mutant almost identically to the trm8Δ rpl502-Y44X mutant, with similar growth in YES and EMMC media at high temperature, and similar 5-FU sensitivity and 3-AT resistance (Fig 2A)(Figs 1A) In this way, we identified a group of four trm8Δ suppressors that suppressed trm8Δ temper- ature sensitivity in rich (YES) and complete minimal media lacking histidine (EMMC-His) almost as efficiently as a trm8Δ suppressor with a representative dhp1 mutation (trm8Δ dhp1-W326L) or GAAC mutation (trm8Δ gcn2-M1I) ------- COMMENT: 36502c2f15fe4801 43 aO+E8SILr3iStpwJ4sEVU63lw7g We found that a reconstructed trm8Δ rpl502Δ strain suppressed the growth defect of a trm8Δ mutant almost identically to the trm8Δ rpl502-Y44X mutant, with similar growth in YES and EMMC media at high temperature, and similar 5-FU sensitivity and 3-AT resistance (Fig 2A) ------- COMMENT: 36502c2f15fe4801 44 aO+E8SILr3iStpwJ4sEVU63lw7g We found that a reconstructed trm8Δ rpl502Δ strain suppressed the growth defect of a trm8Δ mutant almost identically to the trm8Δ rpl502-Y44X mutant, with similar growth in YES and EMMC media at high temperature, and similar 5-FU sensitivity and 3-AT resistance (Fig 2A) ------- COMMENT: 36502c2f15fe4801 45 +4eiPyGvALyNs+aL9HU0dluRClY whereas levels of tRNATyr(GUA) were modestly restored in trm8Δ gcn2Δ mutants (58% vs 41%), as we observed previously [23] (Fig 2B and 2C). ------- COMMENT: 36502c2f15fe4801 46 xixV7l3eIvg2805vq++xdaKOuwI (Fig 1B and 1C). ------- COMMENT: 36502c2f15fe4801 47 J+H7jbaHpxQftk0fHfpeA8fhQ+8 (Fig 1B and 1C) ------- COMMENT: 36502c2f15fe4801 48 J+H7jbaHpxQftk0fHfpeA8fhQ+8 (Fig 1B and 1C) ------- COMMENT: 36502c2f15fe4801 49 cHbJnNnE5xK8MpeZiZnnwmSygSY (Fig 1B and 1C). Similarly, tRNAPro(AGG) levels were not efficiently restored in trm8Δ rpl502Δ mutants (38%) relative to 25% in trm8Δ mutants and 49% in trm8Δ gcn2Δ strains. ------- COMMENT: 36502c2f15fe4801 50 sRXoP4GSSeXDw+TBVOLW/Irk4/w Furthermore, like trm8Δ rpl502-Y44X mutants, trm8Δ rpl502Δ mutants efficiently suppressed the GAAC activa- tion observed in trm8Δ mutants, measured by relative lys4+ levels (from a 14.5-fold increase in trm8Δ mutants, to a 1.9-fold increase in trm8Δ rpl502Δ mutants), compared to a near baseline 1.2-fold increase in a trm8Δ gcn2Δ strain (Figs 2D and S5). ------- COMMENT: 36502c2f15fe4801 51 2gMo7blOp/e3kjz/Kv1XyLVFnQ4 Deletion of each of the two rps genes (rps2801Δ and rps802Δ, encoding Rps28 and Rps8) and four rpl genes examined (rpl1601Δ, rpl1202Δ, rpl2802Δ, and rpl1701Δ, encoding Rpl16, Rpl12, Rpl28, and Rpl17) resulted in efficient sup- pression of the trm8Δ temperature sensitivity in EMMC-His media, although suppression was somewhat weaker for the rpl1601Δ mutation (Fig 3A and 3B) ------- COMMENT: 36502c2f15fe4801 52 rQTuuRNuI2VbJUOBHfFy2Jbv15o (vw: growth seems slightly reduced compared to WT) Deletion of each of the two rps genes (rps2801Δ and rps802Δ, encoding Rps28 and Rps8) and four rpl genes examined (rpl1601Δ, rpl1202Δ, rpl2802Δ, and rpl1701Δ, encoding Rpl16, Rpl12, Rpl28, and Rpl17) resulted in efficient sup- pression of the trm8Δ temperature sensitivity in EMMC-His media, although suppression was somewhat weaker for the rpl1601Δ mutation (Fig 3A and 3B) ------- COMMENT: 36502c2f15fe4801 53 2gMo7blOp/e3kjz/Kv1XyLVFnQ4 Deletion of each of the two rps genes (rps2801Δ and rps802Δ, encoding Rps28 and Rps8) and four rpl genes examined (rpl1601Δ, rpl1202Δ, rpl2802Δ, and rpl1701Δ, encoding Rpl16, Rpl12, Rpl28, and Rpl17) resulted in efficient sup- pression of the trm8Δ temperature sensitivity in EMMC-His media, although suppression was somewhat weaker for the rpl1601Δ mutation (Fig 3A and 3B) ------- COMMENT: 36502c2f15fe4801 54 2gMo7blOp/e3kjz/Kv1XyLVFnQ4 Deletion of each of the two rps genes (rps2801Δ and rps802Δ, encoding Rps28 and Rps8) and four rpl genes examined (rpl1601Δ, rpl1202Δ, rpl2802Δ, and rpl1701Δ, encoding Rpl16, Rpl12, Rpl28, and Rpl17) resulted in efficient sup- pression of the trm8Δ temperature sensitivity in EMMC-His media, although suppression was somewhat weaker for the rpl1601Δ mutation (Fig 3A and 3B) ------- COMMENT: 36502c2f15fe4801 55 2gMo7blOp/e3kjz/Kv1XyLVFnQ4 Deletion of each of the two rps genes (rps2801Δ and rps802Δ, encoding Rps28 and Rps8) and four rpl genes examined (rpl1601Δ, rpl1202Δ, rpl2802Δ, and rpl1701Δ, encoding Rpl16, Rpl12, Rpl28, and Rpl17) resulted in efficient sup- pression of the trm8Δ temperature sensitivity in EMMC-His media, although suppression was somewhat weaker for the rpl1601Δ mutation (Fig 3A and 3B) ------- COMMENT: 36502c2f15fe4801 56 D6TKYBohV/0QnNA2roExg4UY5g4 Similarly, all five of the trm8Δ rplΔ and trm8Δ rpsΔ mutants that were robust trm8Δ suppressors were also sensitive on YES +5-FU media at 33 ̊C, whereas the weak trm8Δ rpl1601Δ suppressor was slightly sensitive on YES+5-FU. ------- COMMENT: 36502c2f15fe4801 57 D6TKYBohV/0QnNA2roExg4UY5g4 Similarly, all five of the trm8Δ rplΔ and trm8Δ rpsΔ mutants that were robust trm8Δ suppressors were also sensitive on YES +5-FU media at 33 ̊C, whereas the weak trm8Δ rpl1601Δ suppressor was slightly sensitive on YES+5-FU. ------- COMMENT: 36502c2f15fe4801 58 D6TKYBohV/0QnNA2roExg4UY5g4 Similarly, all five of the trm8Δ rplΔ and trm8Δ rpsΔ mutants that were robust trm8Δ suppressors were also sensitive on YES +5-FU media at 33 ̊C, whereas the weak trm8Δ rpl1601Δ suppressor was slightly sensitive on YES+5-FU. ------- COMMENT: 36502c2f15fe4801 59 D6TKYBohV/0QnNA2roExg4UY5g4 Similarly, all five of the trm8Δ rplΔ and trm8Δ rpsΔ mutants that were robust trm8Δ suppressors were also sensitive on YES +5-FU media at 33 ̊C, whereas the weak trm8Δ rpl1601Δ suppressor was slightly sensitive on YES+5-FU. ------- COMMENT: 36502c2f15fe4801 60 D6TKYBohV/0QnNA2roExg4UY5g4 Similarly, all five of the trm8Δ rplΔ and trm8Δ rpsΔ mutants that were robust trm8Δ suppressors were also sensitive on YES +5-FU media at 33 ̊C, whereas the weak trm8Δ rpl1601Δ suppressor was slightly sensitive on YES+5-FU. ------- COMMENT: 36502c2f15fe4801 61 lqKgAUddf9dlJoUCBfNh1stC2rQ (fig 3) By contrast, we found that 3-AT resistance was not correlated with the efficiency of sup- pression in trm8Δ rplΔ strain, as one of the robust trm8Δ suppressors was almost completely 3-AT sensitive (trm8Δ rpl1202Δ) and two other robust suppressors were only moderately 3-AT resistant (trm8Δ rps2801Δ and trm8Δ rps802Δ), whereas the robust trm8Δ rplΔ2802Δ and trm8Δ rpl1701Δ suppressor strains were strongly 3-AT resistant. This result is consistent with our finding above that 3-AT resistance is a property of the mutation in the ribosomal pro- tein gene, and not the trm8Δ mutation, However, it is not immediately clear why some mutations in ribosomal protein genes result in 3-AT resistance and others result in sensitivity, ------- COMMENT: 36502c2f15fe4801 62 lqKgAUddf9dlJoUCBfNh1stC2rQ (fig 3) By contrast, we found that 3-AT resistance was not correlated with the efficiency of sup- pression in trm8Δ rplΔ strain, as one of the robust trm8Δ suppressors was almost completely 3-AT sensitive (trm8Δ rpl1202Δ) and two other robust suppressors were only moderately 3-AT resistant (trm8Δ rps2801Δ and trm8Δ rps802Δ), whereas the robust trm8Δ rplΔ2802Δ and trm8Δ rpl1701Δ suppressor strains were strongly 3-AT resistant. This result is consistent with our finding above that 3-AT resistance is a property of the mutation in the ribosomal pro- tein gene, and not the trm8Δ mutation, However, it is not immediately clear why some mutations in ribosomal protein genes result in 3-AT resistance and others result in sensitivity, ------- COMMENT: 36502c2f15fe4801 63 lqKgAUddf9dlJoUCBfNh1stC2rQ (fig 3) By contrast, we found that 3-AT resistance was not correlated with the efficiency of sup- pression in trm8Δ rplΔ strain, as one of the robust trm8Δ suppressors was almost completely 3-AT sensitive (trm8Δ rpl1202Δ) and two other robust suppressors were only moderately 3-AT resistant (trm8Δ rps2801Δ and trm8Δ rps802Δ), whereas the robust trm8Δ rplΔ2802Δ and trm8Δ rpl1701Δ suppressor strains were strongly 3-AT resistant. This result is consistent with our finding above that 3-AT resistance is a property of the mutation in the ribosomal pro- tein gene, and not the trm8Δ mutation, However, it is not immediately clear why some mutations in ribosomal protein genes result in 3-AT resistance and others result in sensitivity, ------- COMMENT: 36502c2f15fe4801 64 lqKgAUddf9dlJoUCBfNh1stC2rQ (fig 3) By contrast, we found that 3-AT resistance was not correlated with the efficiency of sup- pression in trm8Δ rplΔ strain, as one of the robust trm8Δ suppressors was almost completely 3-AT sensitive (trm8Δ rpl1202Δ) and two other robust suppressors were only moderately 3-AT resistant (trm8Δ rps2801Δ and trm8Δ rps802Δ), whereas the robust trm8Δ rplΔ2802Δ and trm8Δ rpl1701Δ suppressor strains were strongly 3-AT resistant. This result is consistent with our finding above that 3-AT resistance is a property of the mutation in the ribosomal pro- tein gene, and not the trm8Δ mutation, However, it is not immediately clear why some mutations in ribosomal protein genes result in 3-AT resistance and others result in sensitivity, ------- COMMENT: 36502c2f15fe4801 65 D6TKYBohV/0QnNA2roExg4UY5g4 Similarly, all five of the trm8Δ rplΔ and trm8Δ rpsΔ mutants that were robust trm8Δ suppressors were also sensitive on YES +5-FU media at 33 ̊C, whereas the weak trm8Δ rpl1601Δ suppressor was slightly sensitive on YES+5-FU. ------- COMMENT: 36502c2f15fe4801 66 2gMo7blOp/e3kjz/Kv1XyLVFnQ4 Deletion of each of the two rps genes (rps2801Δ and rps802Δ, encoding Rps28 and Rps8) and four rpl genes examined (rpl1601Δ, rpl1202Δ, rpl2802Δ, and rpl1701Δ, encoding Rpl16, Rpl12, Rpl28, and Rpl17) resulted in efficient sup- pression of the trm8Δ temperature sensitivity in EMMC-His media, although suppression was somewhat weaker for the rpl1601Δ mutation (Fig 3A and 3B) ------- COMMENT: 36502c2f15fe4801 67 5+5fnGJquYNjCaoREmZhZLGkZvk (Figure 4 B) S. pombe tan1Δ mutants are temperature sensitive due to decay of two tRNA species by the RTD pathway. ------- COMMENT: 36502c2f15fe4801 68 MYZRpg2p8mn2jOds9K6SCEteUpI (figure 4 C) Consistent with the biology of Tan1 in S. cerevisiae, we find that S. pombe tan1Δ mutants are temperature sensitive due to decay of two tRNA species by the RTD pathway. ------- COMMENT: 36502c2f15fe4801 69 MYZRpg2p8mn2jOds9K6SCEteUpI (figure 4 C) Consistent with the biology of Tan1 in S. cerevisiae, we find that S. pombe tan1Δ mutants are temperature sensitive due to decay of two tRNA species by the RTD pathway. ------- COMMENT: 36502c2f15fe4801 70 qW1l/Ghiy88aaawzsB1GfwCpfSA (figure 4 C) As antici- pated based on the conservation of Tan1 sequence and the Tan1 requirement for ac4C12 modi- fication of tRNALeu and tRNASer within a CCG motif [35–38], purified tRNALeu(CAA) from S. pombe tan1Δ mutants lacks any detectable ac4C, compared to that in WT cells, but has similar levels of four control modifications (Fig 4A). ------- COMMENT: 36502c2f15fe4801 71 Xe1Chj45g7OnZJp4eOv4U6ZyWuU phenotypes support conservation of role in S. cerevisiae which is binding tRNA and "The interaction of NAT10 and THUMPD1 suggests that they work together in tRNA acetylation and that THUMPD1 acts as a specific tRNA adaptor. In agreement with this view, we found Kre33 and Tan1 to interact in a 2-hybrid assay in budding yeast cells (Figure 5J). ------- COMMENT: 36502c2f15fe4801 72 Xe1Chj45g7OnZJp4eOv4U6ZyWuU (comment: phenotypes support conservation of role in S. cerevisiae which is binding tRNA and) "The interaction of NAT10 and THUMPD1 suggests that they work together in tRNA acetylation and that THUMPD1 acts as a specific tRNA adaptor. In agreement with this view, we found Kre33 and Tan1 to interact in a 2-hybrid assay in budding yeast cells (Figure 5J). ------- COMMENT: 36502c2f15fe4801 73 1+RRB855kKYJx6RizbBnfqeaBwo rpl2802Δ mutation efficiently suppresses the growth phenotypes of an S. pombe tan1Δ mutant, resulting in temperature resistance on EMMC-His media and YES media at 38 ̊C, 3-AT resistance at 35 ̊C ..... (Fig 5A). ------- COMMENT: 36502c2f15fe4801 74 1+RRB855kKYJx6RizbBnfqeaBwo rpl2802Δ mutation efficiently suppresses the growth phenotypes of an S. pombe tan1Δ mutant, resulting in temperature resistance on EMMC-His media and YES media at 38 ̊C, 3-AT resistance at 35 ̊C ..... (Fig 5A). ------- COMMENT: 36502c2f15fe4801 75 C+yjTbwSKI9vWYTKIOcq3hbQKsM (Figure 4 B) S. pombe tan1Δ mutants are temperature sensitive due to decay of two tRNA species by the RTD pathway. ------- COMMENT: 36502c2f15fe4801 76 Uay/vo/eRksuQPMFKm62qPiq3R4 rpl2802Δ mutation efficiently suppresses the growth phenotypes of an S. pombe tan1Δ mutant, resulting in temperature resistance on EMMC-His media and YES media at 38 ̊C, 3-AT resistance at 35 ̊C .....Fig 5A). ------- COMMENT: 36502c2f15fe4801 77 BoMPck+IF8zVxZfFBmCBmIaTo70 As we saw for the trm8Δ rpl502Δ strain, we find that the tan1Δ rpl502Δ strain does not have restored tRNA levels after growth at 39 ̊C (Fig 6A and 6B).... Relative tRNALeu(AAG) levels are reduced substantially in tan1Δ strains after growth at 39 ̊C (to 24% of WT), ------- COMMENT: 36502c2f15fe4801 78 VxXhHULUPg7m1OQfTnT7ZwQOhWw (Fig 6A and 6B) Relative tRNALeu(AAG) levels are reduced substantially in tan1Δ strains after growth at 39 ̊C (to 24% of WT) ------- COMMENT: 36502c2f15fe4801 79 vixDpRpCtgDklz+ETPoDm46N2vs However, we find that although an S. pombe tan1Δ mutant activates the GAAC pathway, based on analysis of relative lys4+ mRNA levels, the activation is weak, and is only modestly reduced in a tan1Δ rpl502Δ strain (Fig 6C). ------- COMMENT: 36502c2f15fe4801 80 bwyK/A9emjFNH0CuLc9kpvCFkPw These weak effects of the tan1Δ mutant and the tan1Δ rpl502Δ mutant on GAAC activation are consistent with the observation that an rpl502Δ mutation is a more efficient suppressor of the temperature sensitivity of an S. pombe tan1Δ mutant than is a gcn2Δ mutation, on either YES media or EMMC-His media at 38 ̊C (Fig 6D). ------- COMMENT: 36502c2f15fe4801 81 bwyK/A9emjFNH0CuLc9kpvCFkPw These weak effects of the tan1Δ mutant and the tan1Δ rpl502Δ mutant on GAAC activation are consistent with the observation that an rpl502Δ mutation is a more efficient suppressor of the temperature sensitivity of an S. pombe tan1Δ mutant than is a gcn2Δ mutation, on either YES media or EMMC-His media at 38 ̊C (Fig 6D). ------- COMMENT: 36502c2f15fe4801 82 bwyK/A9emjFNH0CuLc9kpvCFkPw These weak effects of the tan1Δ mutant and the tan1Δ rpl502Δ mutant on GAAC activation are consistent with the observation that an rpl502Δ mutation is a more efficient suppressor of the temperature sensitivity of an S. pombe tan1Δ mutant than is a gcn2Δ mutation, on either YES media or EMMC-His media at 38 ̊C (Fig 6D). ------- COMMENT: 36502c2f15fe4801 83 bwyK/A9emjFNH0CuLc9kpvCFkPw These weak effects of the tan1Δ mutant and the tan1Δ rpl502Δ mutant on GAAC activation are consistent with the observation that an rpl502Δ mutation is a more efficient suppressor of the temperature sensitivity of an S. pombe tan1Δ mutant than is a gcn2Δ mutation, on either YES media or EMMC-His media at 38 ̊C (Fig 6D). ------- COMMENT: 36502c2f15fe4801 84 Tc54Heh3upT23e7BwmpYo5XDtRw Con- sistent with a defect in translation due to loss of ribosomal protein paralogs, we find that each of four rplΔ mutants and three rpsΔ mutants has a substantially reduced growth rate (increased generation time) relative to that of the WT strain in YES media at 30 ̊C (Fig 7A and 7B and S1 Table). ------- COMMENT: 36502c2f15fe4801 85 Tc54Heh3upT23e7BwmpYo5XDtRw Con- sistent with a defect in translation due to loss of ribosomal protein paralogs, we find that each of four rplΔ mutants and three rpsΔ mutants has a substantially reduced growth rate (increased generation time) relative to that of the WT strain in YES media at 30 ̊C (Fig 7A and 7B and S1 Table). ------- COMMENT: 36502c2f15fe4801 86 Tc54Heh3upT23e7BwmpYo5XDtRw Con- sistent with a defect in translation due to loss of ribosomal protein paralogs, we find that each of four rplΔ mutants and three rpsΔ mutants has a substantially reduced growth rate (increased generation time) relative to that of the WT strain in YES media at 30 ̊C (Fig 7A and 7B and S1 Table). ------- COMMENT: 36502c2f15fe4801 87 Tc54Heh3upT23e7BwmpYo5XDtRw Con- sistent with a defect in translation due to loss of ribosomal protein paralogs, we find that each of four rplΔ mutants and three rpsΔ mutants has a substantially reduced growth rate (increased generation time) relative to that of the WT strain in YES media at 30 ̊C (Fig 7A and 7B and S1 Table). ------- COMMENT: 36502c2f15fe4801 88 Tc54Heh3upT23e7BwmpYo5XDtRw Con- sistent with a defect in translation due to loss of ribosomal protein paralogs, we find that each of four rplΔ mutants and three rpsΔ mutants has a substantially reduced growth rate (increased generation time) relative to that of the WT strain in YES media at 30 ̊C (Fig 7A and 7B and S1 Table). ------- COMMENT: 36502c2f15fe4801 89 Tc54Heh3upT23e7BwmpYo5XDtRw Con- sistent with a defect in translation due to loss of ribosomal protein paralogs, we find that each of four rplΔ mutants and three rpsΔ mutants has a substantially reduced growth rate (increased generation time) relative to that of the WT strain in YES media at 30 ̊C (Fig 7A and 7B and S1 Table). ------- COMMENT: 36502c2f15fe4801 90 Tc54Heh3upT23e7BwmpYo5XDtRw Con- sistent with a defect in translation due to loss of ribosomal protein paralogs, we find that each of four rplΔ mutants and three rpsΔ mutants has a substantially reduced growth rate (increased generation time) relative to that of the WT strain in YES media at 30 ̊C (Fig 7A and 7B and S1 Table). ------- COMMENT: 36502c2f15fe4801 91 Tc54Heh3upT23e7BwmpYo5XDtRw Con- sistent with a defect in translation due to loss of ribosomal protein paralogs, we find that each of four rplΔ mutants and three rpsΔ mutants has a substantially reduced growth rate (increased generation time) relative to that of the WT strain in YES media at 30 ̊C (Fig 7A and 7B and S1 Table). ------- COMMENT: 36502c2f15fe4801 92 XWuNZNM9zJjkNN1m1nZN4eH23Xk Polysome profiling of three of these mutants provides additional evidence for a defect in trans- lation in the mutants (Fig 7C). ------- COMMENT: 36502c2f15fe4801 93 XWuNZNM9zJjkNN1m1nZN4eH23Xk Polysome profiling of three of these mutants provides additional evidence for a defect in trans- lation in the mutants (Fig 7C). ------- COMMENT: 36502c2f15fe4801 94 9vOeNxsUDyHEfeXhaRoiceDjcWc Moreover, both rplΔ mutant profiles, but not the WT, exhibit substantial populations of halfmers, monosomes and polysomes with an additional 40S subunit ------- COMMENT: 36502c2f15fe4801 95 9vOeNxsUDyHEfeXhaRoiceDjcWc Moreover, both rplΔ mutant profiles, but not the WT, exhibit substantial populations of halfmers, monosomes and polysomes with an additional 40S subunit ------- COMMENT: 36502c2f15fe4801 96 oHrdYnE9N1Vy1XCIW1JvkLvW8x4 The rps23Δ mutant also has reduced polysome density, indicative of reduced translation, accompanied by an undetectable 40S peak and a greatly pronounced 60S peak that substantially overshadows the 80 monosome peak ------- COMMENT: 36502c2f15fe4801 97 oHrdYnE9N1Vy1XCIW1JvkLvW8x4 The rps23Δ mutant also has reduced polysome density, indicative of reduced translation, accompanied by an undetectable 40S peak and a greatly pronounced 60S peak that substantially overshadows the 80 monosome peak ------- COMMENT: 36502c2f15fe4801 98 XWuNZNM9zJjkNN1m1nZN4eH23Xk Polysome profiling of three of these mutants provides additional evidence for a defect in trans- lation in the mutants (Fig 7C). ------- COMMENT: 36502c2f15fe4801 99 XWuNZNM9zJjkNN1m1nZN4eH23Xk Polysome profiling of three of these mutants provides additional evidence for a defect in trans- lation in the mutants (Fig 7C). ------- COMMENT: 369d95f63c03066b 4 K3nVv1TfKzubU7fYuTZecG8kCAk (comment: At stress response genes) ------- COMMENT: 36d4085e11a5e17d 1 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 36d4085e11a5e17d 2 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 36d4085e11a5e17d 3 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 36d4085e11a5e17d 4 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 36d4085e11a5e17d 5 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 36d4085e11a5e17d 7 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 36d4085e11a5e17d 8 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 36d4085e11a5e17d 9 kDo5Ri90R5yBnnPUx5bjLeOGHDo (comment: Amplified by HU treatment) Fig. 2C and 3A ------- COMMENT: 36d4085e11a5e17d 10 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 36d4085e11a5e17d 11 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 36d4085e11a5e17d 12 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 36d4085e11a5e17d 13 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 36d4085e11a5e17d 14 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 36d4085e11a5e17d 15 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 36d4085e11a5e17d 16 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 36d4085e11a5e17d 17 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 36d4085e11a5e17d 18 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 36d4085e11a5e17d 19 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 36d4085e11a5e17d 20 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 36d4085e11a5e17d 21 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 36d4085e11a5e17d 22 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 36d4085e11a5e17d 23 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 36d4085e11a5e17d 24 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 36d4085e11a5e17d 25 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 36d4085e11a5e17d 26 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 36d4085e11a5e17d 27 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 36d4085e11a5e17d 28 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 36d4085e11a5e17d 29 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 36de390cd2e1923e 26 goEO9S3UHQnaYolcF2Vye6cN6UI (comment: dependent on F-actin, assayed using Latrunculin A) ------- COMMENT: 36de390cd2e1923e 27 zaDFFG/TtjqN0UW96gHS+R7W9qY (comment: also from timing of localization to patches) ------- COMMENT: 36de390cd2e1923e 28 /hssPjVe+cRmHtW5k5qxoAA7ePc (comment: specific Arp2/3 complex subunit(s) not identified; authors use Myo1 tail as representative of whole protein) ------- COMMENT: 36de390cd2e1923e 31 goEO9S3UHQnaYolcF2Vye6cN6UI (dependent on F-actin, assayed using Latrunculin A) ------- COMMENT: 36de390cd2e1923e 32 zaDFFG/TtjqN0UW96gHS+R7W9qY (comment: also from timing of localization to patches) ------- COMMENT: 36de390cd2e1923e 33 /hssPjVe+cRmHtW5k5qxoAA7ePc (comment: specific Arp2/3 complex subunit(s) not identified; authors use Myo1 tail as representative of whole protein) ------- COMMENT: 36de390cd2e1923e 36 goEO9S3UHQnaYolcF2Vye6cN6UI (comment: dependent on F-actin, assayed using Latrunculin A) ------- COMMENT: 36de390cd2e1923e 37 oSAgDcGdKKN9WsHAqKrjV3rRM0A (comment: also from synthetic lethality with myo1, timing of localization to patches, and vrp1 mutant phenotype) ------- COMMENT: 36de390cd2e1923e 38 zaDFFG/TtjqN0UW96gHS+R7W9qY (comment: also from timing of localization to patches) ------- COMMENT: 36fa256918417316 1 UUIGAGYIlzNh3/CZeXrmr/tr93Q RTS1 inversion background abolishes DSB formation; "decreased" level in rtf1-W405G is relative to wild type and above the inverted-RTS1 background level ------- COMMENT: 36fa256918417316 11 fq9boRViz6Wb+0CG0QtVmUbazvo no barrier activity with reversed polarity as in rtf1-S154L single mutant ------- COMMENT: 36fa256918417316 12 fq9boRViz6Wb+0CG0QtVmUbazvo no barrier activity with reversed polarity as in rtf1-S154L single mutant ------- COMMENT: 370824a189ad6763 1 CcSufbFQp136O9RHipdTnr7sNcE Fig 1 cells blocked in G1 at the restrictive temp ------- COMMENT: 370824a189ad6763 2 msbvsxkadTEsKxypvLfAhTxd78s Fig2 ------- COMMENT: 370824a189ad6763 3 msbvsxkadTEsKxypvLfAhTxd78s Fig2 ------- COMMENT: 370824a189ad6763 5 jn42KoF/UIv2XW0Q7N6KgL46d70 Fig2, Fig3 ------- COMMENT: 370824a189ad6763 6 jn42KoF/UIv2XW0Q7N6KgL46d70 Fig2, Fig3 ------- COMMENT: 370824a189ad6763 7 g1W1uUJsMT6QDLHnwVqPer+bM+I Fig4 (comment: cdc13Δ90 binds rum1 in vitro) ------- COMMENT: 370824a189ad6763 10 xAf23A/e9G1YiUMAEQdhIeSVie0 Fig5 ------- COMMENT: 370824a189ad6763 12 NGVCJVNgDZdjzJWHXp7Ym8ef2uo Fig 8 (comment: added by cig1 associated CDK1) ------- COMMENT: 370824a189ad6763 13 mbaEZeRI58HMJUN4tuwYV0r04Yk Fig7B ------- COMMENT: 370824a189ad6763 14 GYFE+Nq1jPfaQlbEyrCoIrIdO9s Fig7A ------- COMMENT: 370824a189ad6763 15 UP/fL2dP9BKhdjQVzQFivTRXXPw Fig 1 (comment: exponentially growing cells mainly in G2) ------- COMMENT: 37237b530ab38bc9 18 4Y0lECtTc9g5LRNpzdRfEP90yfc (comment: mcm4ts-td phenotype indicates that Cdc23 chromatin localization is independent of Mcm4) ------- COMMENT: 37237b530ab38bc9 19 BMLfiDBL1zq1/zzSIRx95pVa5Aw (comment: CHECK ADD? late anaphase) ------- COMMENT: 37a883806f3d3302 6 5mX1z4CHzF6vU50VoKDjsGG7TFI (comment: assayed using 160-bp palindromic sequence inserted into ade6 locus) ------- COMMENT: 37af2321f43c3188 6 USYQ7JzzI8ykc8+ZdiN9zurGbwc An increased level of phosphotyrosine was detected on Spc1 in wis1􏰁 cells overexpressing Wis4∆N, but not in wis1∆ cells, indicating that the action of Wis4 is Wis1- dependent. ------- COMMENT: 37af2321f43c3188 10 NX6v/toclXh9222PH8RUlVRwEAI data not shown ------- COMMENT: 37af2321f43c3188 23 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 37af2321f43c3188 24 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 37af2321f43c3188 25 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 37af2321f43c3188 26 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 37af2321f43c3188 27 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 37af2321f43c3188 28 KexZJLcBsx+PTMTb52RZYuVDR5U (Figure 4) ------- COMMENT: 37af2321f43c3188 29 KexZJLcBsx+PTMTb52RZYuVDR5U (Figure 4) ------- COMMENT: 37af2321f43c3188 32 KexZJLcBsx+PTMTb52RZYuVDR5U (Figure 4) ------- COMMENT: 37af2321f43c3188 33 KexZJLcBsx+PTMTb52RZYuVDR5U (Figure 4) ------- COMMENT: 37b08171d9751598 1 goEO9S3UHQnaYolcF2Vye6cN6UI (comment: dependent on F-actin, assayed using Latrunculin A) ------- COMMENT: 37b08171d9751598 30 /x8f+qA3cqDfjGKY39WS6zIWlEw data from table; nothing more specific shown ------- COMMENT: 37b08171d9751598 31 /x8f+qA3cqDfjGKY39WS6zIWlEw data from table; nothing more specific shown ------- COMMENT: 37b08171d9751598 32 /x8f+qA3cqDfjGKY39WS6zIWlEw data from table; nothing more specific shown ------- COMMENT: 37b08171d9751598 33 /x8f+qA3cqDfjGKY39WS6zIWlEw data from table; nothing more specific shown ------- COMMENT: 37b08171d9751598 34 /x8f+qA3cqDfjGKY39WS6zIWlEw data from table; nothing more specific shown ------- COMMENT: 37b94fa89fda5a2d 1 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: 37cb96e688f6a42c 1 8jTjtkNnNoFhiY57qk4SkW6skr0 figure 1A ------- COMMENT: 37cb96e688f6a42c 3 8jTjtkNnNoFhiY57qk4SkW6skr0 figure 1A ------- COMMENT: 37cb96e688f6a42c 4 8jTjtkNnNoFhiY57qk4SkW6skr0 figure 1A ------- COMMENT: 37cb96e688f6a42c 5 8jTjtkNnNoFhiY57qk4SkW6skr0 figure 1A ------- COMMENT: 37cb96e688f6a42c 6 8jTjtkNnNoFhiY57qk4SkW6skr0 figure 1A ------- COMMENT: 37cb96e688f6a42c 8 8P3+s/LHx228Teo2hvMK0TsnkX8 figure 2A/B ------- COMMENT: 37cb96e688f6a42c 12 pwcwASlWQtRBBoormzj5gHYObdE fig 4A ------- COMMENT: 37cb96e688f6a42c 13 +lGokglJTKvk0vvTDRIpieifdEo figure 4B ------- COMMENT: 37cb96e688f6a42c 14 VdLBeBTW4N6KstLu6xXkj8OAQW4 30x figure 4B ------- COMMENT: 37ebf9ba3b26613d 1 g7uudDzcT0At87Gt2VXbeaRdeYk (comment: Fml1 binds to the four-way junction at a displacement (D) loop.) ------- COMMENT: 37ebf9ba3b26613d 53 JJBXD8m9YHAXxanrP4MJFDRnwrs (comment: Fml1 catalyses the dissociation of displacement (D) loops) ------- COMMENT: 383a6e4a068f07e8 1 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: 383a6e4a068f07e8 2 RUud/EiezmXnHBvUHfHqO5rtOvc (Fig. 6A and B) ------- COMMENT: 383a6e4a068f07e8 3 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: 383a6e4a068f07e8 4 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: 383a6e4a068f07e8 5 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: 383a6e4a068f07e8 6 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: 383a6e4a068f07e8 7 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 383a6e4a068f07e8 8 gmqogAnY3BQANKEbZmiyaveRfrg (Fig. 3E) ------- COMMENT: 388045986ed63280 1 8enZpakx7x07WGSbeHowntCdlyo they ceased to increase in number after two divisions (Fig. 1A, open triangles). The growth arrest phenotype of mis3-224 (Fig. 1C) was distinct from that of typical cdc mutants, as its cell length increase was insigni®cant (1.3-fold) after 8h at 36 8C. mis3-224 at 36 8C was unable to increase in cell length because the levels of protein and RNA ceased to increase (B, ®lled and open triangles, respectively). ------- COMMENT: 388045986ed63280 2 8enZpakx7x07WGSbeHowntCdlyo they ceased to increase in number after two divisions (Fig. 1A, open triangles). The growth arrest phenotype of mis3-224 (Fig. 1C) was distinct from that of typical cdc mutants, as its cell length increase was insigni®cant (1.3-fold) after 8h at 36 8C. mis3-224 at 36 8C was unable to increase in cell length because the levels of protein and RNA ceased to increase (B, ®lled and open triangles, respectively). ------- COMMENT: 388045986ed63280 3 9vci+plP6FQHTFUSEUSia/Iczz8 These results showed that cells lacking functional Mis3 could not promote cell growth upon the release to complete medium and remained in the G1/S phase. ------- COMMENT: 388045986ed63280 9 K/LWbrqnyVNeTFdQnRwfU8co75s it was moderately sensitive at 308C (Fig. 5A, top): mutant failed to produce colonies in the presence of 8 mM HU at 30 8C, a semi-restrictive temperature. ------- COMMENT: 388045986ed63280 10 5ARd55Wx22Q1L+Q5SgdGMjW+jT0 At 36 8C in mis3-224, the level of Mik1 increased to a peak after 4h, but then strikingly decreased to a low level (E). ------- COMMENT: 388045986ed63280 11 5ARd55Wx22Q1L+Q5SgdGMjW+jT0 At 36 8C in mis3-224, the level of Mik1 increased to a peak after 4h, but then strikingly decreased to a low level (E). ------- COMMENT: 388045986ed63280 12 MVCwOubcgx8UtaJ92Q2kjCu13/I Tetrad dissection indicated that the mis3-224 dsk1 null double mutant failed to grow at any temperature. ------- COMMENT: 388045986ed63280 13 cg4gBT3eelqU7bhMX1aviNku6gw In addition to the interaction between Mis3 and Wee1, it was found that the double mutants mis3 cdc25 and mis3 cdc13 were able to form colonies at 268C, but not at 308C (Fig. 6A). ------- COMMENT: 388045986ed63280 14 cg4gBT3eelqU7bhMX1aviNku6gw In addition to the interaction between Mis3 and Wee1, it was found that the double mutants mis3 cdc25 and mis3 cdc13 were able to form colonies at 268C, but not at 308C (Fig. 6A). ------- COMMENT: 388045986ed63280 15 mpz+lgj8hMKPhkuxrFw5EB2b5DY We found, on the other hand, that the ts phenotype of mis3-224 was suppressed by dis2-11, a cs mutation with greatly reduced type 1 protein phosphatase (PP1) activity and the inability to exit from mitosis (Ohkura et al. 1989). ------- COMMENT: 389e7e262d4752a3 28 MsrhmUHw3VmSwkiHiQ2Z1EO0rgM (comment: >50% activity) ------- COMMENT: 38a5be55b66fa29b 1 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: 38a5be55b66fa29b 3 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: 38a5be55b66fa29b 4 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: 38a5be55b66fa29b 7 GGcvPT5AE3l5kvrPf+50zs2Br3Q fig 3B (comment: COMMENT CHECK asymettrically localized septum) ------- COMMENT: 38a5be55b66fa29b 8 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: 38a5be55b66fa29b 9 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: 38a5be55b66fa29b 10 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: 38a5be55b66fa29b 13 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: 38a5be55b66fa29b 15 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: 38a5be55b66fa29b 16 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: 38a5be55b66fa29b 18 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 38a5be55b66fa29b 19 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 38a5be55b66fa29b 20 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 38a5be55b66fa29b 21 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 38a5be55b66fa29b 22 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 38a5be55b66fa29b 23 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 38a5be55b66fa29b 24 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 38c6b56f199ef8a0 1 sYYiZOcIDS3S4Ek68eiPs4c+j/M Table 2 Figures 5a–d and S2) ------- COMMENT: 38c6b56f199ef8a0 2 FMDzEFwAgdWe2tyW58ksL0wCSgc (comment: both deletions. blt1 Elongation phenotype supressed) ------- COMMENT: 38c6b56f199ef8a0 3 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: 38c6b56f199ef8a0 4 leb16GgPXiX+Yzo4mw+1nLCdO7s Furthermore, abnormally elon- gated cytoplasmic and spindle MTs were frequently observed in these cells (Figure 6). ------- COMMENT: 38c6b56f199ef8a0 5 YBsaO0Gzy5Iavf8E+ye0FUTjroc Fig 4a ------- COMMENT: 38c6b56f199ef8a0 6 c0uMiNqcE3BgpszPax9cHtCbVbg fig 4d ------- COMMENT: 38c6b56f199ef8a0 7 xNqzhJzcNskn37m/3eRDwsszxuk fig. 4e ------- COMMENT: 38c6b56f199ef8a0 8 xNqzhJzcNskn37m/3eRDwsszxuk fig. 4e ------- COMMENT: 38c6b56f199ef8a0 9 6+sPzWz72fUZPd9iqwML6Q/7g5I (Table 3) ------- COMMENT: 38c6b56f199ef8a0 13 vjGgZbDDEGMMtZ0f5hyRkFjdAVg Fig 4b ------- COMMENT: 38c6b56f199ef8a0 14 J/u73NJLZHqo2KszQYHGVo8QFvU Figure S1a ------- COMMENT: 38c6b56f199ef8a0 15 J/u73NJLZHqo2KszQYHGVo8QFvU Figure S1a ------- COMMENT: 38c6b56f199ef8a0 16 J/u73NJLZHqo2KszQYHGVo8QFvU Figure S1a ------- COMMENT: 38c6b56f199ef8a0 17 J/u73NJLZHqo2KszQYHGVo8QFvU Figure S1a ------- COMMENT: 38c6b56f199ef8a0 18 J/u73NJLZHqo2KszQYHGVo8QFvU Figure S1a ------- COMMENT: 38c6b56f199ef8a0 19 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: 38c6b56f199ef8a0 20 leb16GgPXiX+Yzo4mw+1nLCdO7s Furthermore, abnormally elon- gated cytoplasmic and spindle MTs were frequently observed in these cells (Figure 6). ------- COMMENT: 38c6b56f199ef8a0 21 owoQMOffrXW5Zizu2tAmAcvkgAY Table 3 ------- COMMENT: 38c6b56f199ef8a0 22 lTox+8cuWiP6YAsE/19AK7ePUWw Figure 7b ------- COMMENT: 38c6b56f199ef8a0 23 YBsaO0Gzy5Iavf8E+ye0FUTjroc Fig 4a ------- COMMENT: 38c6b56f199ef8a0 24 jlKdZ1XvezijQ0AzMAeYNKw2vnQ Fig 4e ------- COMMENT: 38d3a04a24fe3f3c 1 xz0at1N2/1hKVbUr9zLikU0bRh4 (Figure 3A) 25 µM of iron chelator bathophenanthroline disulfonate (BPS) was added to YES media to create iron-depleted condition. ------- COMMENT: 38d3a04a24fe3f3c 2 xz0at1N2/1hKVbUr9zLikU0bRh4 (Figure 3A) 25 µM of iron chelator bathophenanthroline disulfonate (BPS) was added to YES media to create iron-depleted condition. ------- COMMENT: 38d3a04a24fe3f3c 3 IfDGp/QVIYs2vPaL1SiDSRWR7UY (Figure 3A) Fe2(SO4)3 was added to YES media for a final concentration of 2.75 mM. ------- COMMENT: 38d3a04a24fe3f3c 4 IfDGp/QVIYs2vPaL1SiDSRWR7UY (Figure 3A) Fe2(SO4)3 was added to YES media for a final concentration of 2.75 mM. ------- COMMENT: 38d3a04a24fe3f3c 5 ssETGQ9/uyDn1bS18/cQ8HymJEM We found that only Δfio1 cells were sensitive to Cu2+ ------- COMMENT: 38d3a04a24fe3f3c 18 HQ+4Ahr8tmlvwr+tFBbJaaSHZEs deletion of frp1 could rescue the sensitivity of the deletion of fio1 cells on Cu2+ medium. ------- COMMENT: 38ddd4fc7a5de776 1 9BFlC1b9kbs4BtaKURSuOlCpv9g (comment: inferred from cell wall galactomannan defects) ------- COMMENT: 38ddd4fc7a5de776 7 kghadVO/skrQJTJ6QpMdzXbhPC0 (comment: inferred from cell wall galactomannan defects) ------- COMMENT: 38ddd4fc7a5de776 23 sbWb54B0y0C32a/ZsyO2C0qYMi4 endoplasmic reticulum quality control of glycoprotein folding The quality control of glycoprotein folding is a process that facilitates glycoprotein folding and retains in the endoplasmic reticulum folding intermediates. ------- COMMENT: 38ddd4fc7a5de776 27 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 38ddd4fc7a5de776 28 HJO993Z6zbE54JcVehqMrffSBQw fig1d ------- COMMENT: 38ddd4fc7a5de776 30 E2f+pLttlD3Zl6eaT4sfL9+MAfQ (comment: CHECK see ttps://github.com/pombase/fypo/issues/3152#issuecomment-340506554) A reduced UDP-glucose transport is a decreased transporter activity that produces an abnormal lower level of UDP-glucose in the endoplasmic reticulum lumen ------- COMMENT: 38ddd4fc7a5de776 31 HJO993Z6zbE54JcVehqMrffSBQw fig1d ------- COMMENT: 38ddd4fc7a5de776 32 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 38ddd4fc7a5de776 33 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 38ddd4fc7a5de776 34 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 38ddd4fc7a5de776 35 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 38ddd4fc7a5de776 36 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 38ddd4fc7a5de776 37 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 38ddd4fc7a5de776 38 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 38ddd4fc7a5de776 39 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 38ddd4fc7a5de776 40 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 38ddd4fc7a5de776 41 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 38ddd4fc7a5de776 42 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 38ddd4fc7a5de776 43 EH0960i42Cnvg8u5NbjBn4R9uqc ------- COMMENT: 38ddd4fc7a5de776 44 EH0960i42Cnvg8u5NbjBn4R9uqc ------- COMMENT: 38ddd4fc7a5de776 45 EH0960i42Cnvg8u5NbjBn4R9uqc ------- COMMENT: 38ddd4fc7a5de776 46 EH0960i42Cnvg8u5NbjBn4R9uqc ------- COMMENT: 38ddd4fc7a5de776 47 EH0960i42Cnvg8u5NbjBn4R9uqc ------- COMMENT: 38ddd4fc7a5de776 48 EH0960i42Cnvg8u5NbjBn4R9uqc ------- COMMENT: 38ddd4fc7a5de776 49 EH0960i42Cnvg8u5NbjBn4R9uqc ------- COMMENT: 38f42cd29537f6be 1 pEigg1ZZB/P65cmcEt+7JzmU9ho to assess its association with dg and dh ncRNAs as well as the snoRNA snR30. Seb1 displays a strong association with these ncRNAs but not with the act1+ RNA (Fig. 1A). ------- COMMENT: 38f42cd29537f6be 2 pEigg1ZZB/P65cmcEt+7JzmU9ho to assess its association with dg and dh ncRNAs as well as the snoRNA snR30. Seb1 displays a strong association with these ncRNAs but not with the act1+ RNA (Fig. 1A). ------- COMMENT: 38f42cd29537f6be 3 fUimtScXz5OFGLGk9lWgDj2jvDo As expected, Hrr1, an RNAi factor, also associates with dg and dh ncRNAs but not with snR30 or act1+ RNA (Supplemental Fig. S1A). ------- COMMENT: 38f42cd29537f6be 4 zNdy8cX2LlvOzB4ghPXvvj4kQrk A strain harboring a ura4+ reporter gene inserted into the innermost repeat (imr) region of centro- mere 1 displays reduced growth on 5-FOA when harboring the seb1-1 allele (Fig. 1C). I ------- COMMENT: 38f42cd29537f6be 5 BItp6eQz7MBFsKJypBXHiv+iHhY In the ectopic heterochromatic silencing reporter strain, the seb1-1 mutation causes an accumulation of the ura4+ transcript (Fig. 1D) and a strong defect in H3K9me2 at the ura4+ gene (Fig. 1E), supporting the growth defect observed using the 5-FOA assay. ------- COMMENT: 38f42cd29537f6be 6 w/r60nlhCGUnvgMxrbw/qh/rs5g We found that all three mutations are required to produce a silencing defect on 5- FOA medium (Fig. 1B). ------- COMMENT: 38f42cd29537f6be 7 w/r60nlhCGUnvgMxrbw/qh/rs5g We found that all three mutations are required to produce a silencing defect on 5- FOA medium (Fig. 1B). ------- COMMENT: 38f42cd29537f6be 8 w/r60nlhCGUnvgMxrbw/qh/rs5g We found that all three mutations are required to produce a silencing defect on 5- FOA medium (Fig. 1B). ------- COMMENT: 38f42cd29537f6be 9 wnjMaD7iYB9kwWl8gGKocAe38B8 seb1-1 displays normal levels of pericentromeric siRNA accu- mulation (Fig. 2A) ------- COMMENT: 38f42cd29537f6be 10 /MKiasc2pZ9FgjJzCZhzCAFPd4U In con- trast, a catalytically dead Dcr1 mutant (dcr1-R1R2) (Colmenares et al. 2007) displays a dramatic increase in dg and dh transcript levels (Fig. 2B). ------- COMMENT: 38f42cd29537f6be 11 4hSJNJro+32c2O1cmBf1X5TrX1Q We found that all three mutations are required to produce a silencing defect on 5- FOA medium (Fig. 1B). However, the mutant with the three amino acid changes (triple mutant) has a milder silencing defect on 5-FOA when compared with the original seb1-1 mutant. ------- COMMENT: 38f42cd29537f6be 12 tLO5PCLmKhqUgPa6eJg1neEaLtA Indeed, the level of Seb1 protein is lower in the seb1-1 mutant when compared with its level in the wild-type seb1+ strain (Supplemental Fig. S4) ------- COMMENT: 38f42cd29537f6be 13 2U3G+1P5h6ahzVpPpoG+SDIZJJk Strikingly, while the levels of H3K9me2 are reduced by only threefold to fivefold in the single mu- tants, H3K9me2 is virtually abolished in the dcr1-R1R2 seb1-1 double mutant to background levels comparable with those measured in the clr4D mutant (Fig. 2C). ------- COMMENT: 38f42cd29537f6be 14 r1y1P0ryl3N3nGFju3xo1rv2Yw8 (comment: siRNA independent) ------- COMMENT: 38f42cd29537f6be 16 kHnB4O11gIabheuglpN4VT+5d2w while H3K9me2 levels at the dg and dh repeats are modestly reduced in the dcr1-R1R2 single mutant, these levels are virtually eliminated in the dcr1-R1R2 clr3D double mutant, a phenotype strikingly similar to that of the dcr1-R1R2 seb1-1 double mutant (Fig. 3A). ------- COMMENT: 38f42cd29537f6be 17 KHcB/q4xMS1o11hYdSo+NWfyfio Finally, a catalytically dead point mutation in the Clr3 HDAC or the Mit1 ATPase domain also eliminates H3K9me in dcr1-R1R2 cells (Fig. 3A). ------- COMMENT: 38f42cd29537f6be 18 KHcB/q4xMS1o11hYdSo+NWfyfio Finally, a catalytically dead point mutation in the Clr3 HDAC or the Mit1 ATPase domain also eliminates H3K9me in dcr1-R1R2 cells (Fig. 3A). ------- COMMENT: 38f42cd29537f6be 19 GicSiLgVJuGmJHDR0rx9FUyHXbA Furthermore, the elimination of H3K9me2 at dg and dh repeats was also observed in strains that have the dcr1-R1R2 mutation combined with a deletion mutation that eliminates the Clr1, Chp2, or Mit1 subunits of SHREC (Fig. 3A). ------- COMMENT: 38f42cd29537f6be 20 GicSiLgVJuGmJHDR0rx9FUyHXbA Furthermore, the elimination of H3K9me2 at dg and dh repeats was also observed in strains that have the dcr1-R1R2 mutation combined with a deletion mutation that eliminates the Clr1, Chp2, or Mit1 subunits of SHREC (Fig. 3A). ------- COMMENT: 38f42cd29537f6be 21 GicSiLgVJuGmJHDR0rx9FUyHXbA Furthermore, the elimination of H3K9me2 at dg and dh repeats was also observed in strains that have the dcr1-R1R2 mutation combined with a deletion mutation that eliminates the Clr1, Chp2, or Mit1 subunits of SHREC (Fig. 3A). ------- COMMENT: 38f42cd29537f6be 22 r1y1P0ryl3N3nGFju3xo1rv2Yw8 (comment: siRNA independent) ------- COMMENT: 38f42cd29537f6be 23 r1y1P0ryl3N3nGFju3xo1rv2Yw8 (comment: siRNA independent) ------- COMMENT: 38f42cd29537f6be 24 r1y1P0ryl3N3nGFju3xo1rv2Yw8 (comment: siRNA independent) ------- COMMENT: 38f42cd29537f6be 25 r1y1P0ryl3N3nGFju3xo1rv2Yw8 (comment: siRNA independent) ------- COMMENT: 38f42cd29537f6be 26 1/8deE4Hpz2sXt4QHfsAi20foUM To further test whether Seb1 functions in the same pathway as SHREC in promoting H3K9me, we compared H3K9me2 levels at dg and dh repeats of the seb1-1 clr3D double mutant with those of the corresponding single mutants. Significantly, the double and single mutants display very similar levels of H3K9me2, ;20%–30% of wild-type levels (Fig. 3B). ------- COMMENT: 38f42cd29537f6be 27 1/8deE4Hpz2sXt4QHfsAi20foUM To further test whether Seb1 functions in the same pathway as SHREC in promoting H3K9me, we compared H3K9me2 levels at dg and dh repeats of the seb1-1 clr3D double mutant with those of the corresponding single mutants. Significantly, the double and single mutants display very similar levels of H3K9me2, ;20%–30% of wild-type levels (Fig. 3B). ------- COMMENT: 38f42cd29537f6be 28 1/8deE4Hpz2sXt4QHfsAi20foUM To further test whether Seb1 functions in the same pathway as SHREC in promoting H3K9me, we compared H3K9me2 levels at dg and dh repeats of the seb1-1 clr3D double mutant with those of the corresponding single mutants. Significantly, the double and single mutants display very similar levels of H3K9me2, ;20%–30% of wild-type levels (Fig. 3B). ------- COMMENT: 38f42cd29537f6be 29 bPYaOXTdumi0axDlKJpaHTSpmOo Similar results were obtained when comparing H3K9me2 levels at dg and dh repeats of the seb1-1 clr1D double mutant with the levels of the corresponding single mutants (Fig. 3C). T ------- COMMENT: 38f42cd29537f6be 30 bPYaOXTdumi0axDlKJpaHTSpmOo Similar results were obtained when comparing H3K9me2 levels at dg and dh repeats of the seb1-1 clr1D double mutant with the levels of the corresponding single mutants (Fig. 3C). T ------- COMMENT: 38f42cd29537f6be 31 V14BgwYD9bzhmDajPafMYTsobAI Moreover, we found that the seb1-1 mutation increases Pol II occupancy and H3K14 acetylation levels at dg and dh repeats (Figs. 3D,E), ------- COMMENT: 38f42cd29537f6be 32 Rlzzq7xiYi7U9Ln/zM/tDZzr5gU We found that every subunit of SHREC tested (Clr3, Clr1, Mit1, and Chp2) is enriched at both dg and dh repeats. However, in the seb1-1 mutant, this enrichment is abolished or strongly reduced (Figs. 5A–D). ------- COMMENT: 38f42cd29537f6be 33 Rlzzq7xiYi7U9Ln/zM/tDZzr5gU We found that every subunit of SHREC tested (Clr3, Clr1, Mit1, and Chp2) is enriched at both dg and dh repeats. However, in the seb1-1 mutant, this enrichment is abolished or strongly reduced (Figs. 5A–D). ------- COMMENT: 38f42cd29537f6be 34 Rlzzq7xiYi7U9Ln/zM/tDZzr5gU We found that every subunit of SHREC tested (Clr3, Clr1, Mit1, and Chp2) is enriched at both dg and dh repeats. However, in the seb1-1 mutant, this enrichment is abolished or strongly reduced (Figs. 5A–D). ------- COMMENT: 38f42cd29537f6be 35 Rlzzq7xiYi7U9Ln/zM/tDZzr5gU We found that every subunit of SHREC tested (Clr3, Clr1, Mit1, and Chp2) is enriched at both dg and dh repeats. However, in the seb1-1 mutant, this enrichment is abolished or strongly reduced (Figs. 5A–D). ------- COMMENT: 38f42cd29537f6be 36 IoRnBUi8w5o9t9nozm2uMmkF6yQ Together, these data support our hypothesis that Seb1 acts by recruiting SHREC to pericentromeric heterochromatin. ------- COMMENT: 3926aad5386b9286 1 zKF1zc0KZ7l6fm6ho5Cwb91dJ24 penetrance is mentioned in EXP accompanying fig 6A ------- COMMENT: 3926aad5386b9286 5 fiDosn/juMPcv/ucadiMfM9Q39g fig 4a b ------- COMMENT: 3926aad5386b9286 14 6ETquB6qf7XZAg8RDXUoPTp/g3Q Fig 6A ------- COMMENT: 3926aad5386b9286 18 zKF1zc0KZ7l6fm6ho5Cwb91dJ24 penetrance is mentioned in EXP accompanying fig 6A ------- COMMENT: 3926aad5386b9286 19 zKF1zc0KZ7l6fm6ho5Cwb91dJ24 penetrance is mentioned in EXP accompanying fig 6A ------- COMMENT: 3926aad5386b9286 20 zKF1zc0KZ7l6fm6ho5Cwb91dJ24 penetrance is mentioned in EXP accompanying fig 6A ------- COMMENT: 3926aad5386b9286 21 zKF1zc0KZ7l6fm6ho5Cwb91dJ24 penetrance is mentioned in EXP accompanying fig 6A ------- COMMENT: 396866ab84f69995 59 PhgC7ZJCfRm92YoJ/oFw2K29KxU Figure 1G/ figure 2 ------- COMMENT: 396866ab84f69995 60 OWaKBnlfgU8UfOak2GuuhFF9F7o figure 1G ------- COMMENT: 396866ab84f69995 61 OWaKBnlfgU8UfOak2GuuhFF9F7o figure 1G ------- COMMENT: 396866ab84f69995 66 9ydzgNyB4udJzLP4/IT5/w5/jCY figure 2 ------- COMMENT: 396866ab84f69995 118 OWaKBnlfgU8UfOak2GuuhFF9F7o figure 1G ------- COMMENT: 396866ab84f69995 119 OWaKBnlfgU8UfOak2GuuhFF9F7o figure 1G ------- COMMENT: 396866ab84f69995 120 OWaKBnlfgU8UfOak2GuuhFF9F7o figure 1G ------- COMMENT: 396866ab84f69995 122 ZfKpnab/q0P9gMwBv8gkPNMTTiY figure 6c ------- COMMENT: 396866ab84f69995 123 ZfKpnab/q0P9gMwBv8gkPNMTTiY figure 6c ------- COMMENT: 396866ab84f69995 124 ZfKpnab/q0P9gMwBv8gkPNMTTiY figure 6c ------- COMMENT: 396866ab84f69995 127 ZfKpnab/q0P9gMwBv8gkPNMTTiY figure 6c ------- COMMENT: 396866ab84f69995 128 ZfKpnab/q0P9gMwBv8gkPNMTTiY figure 6c ------- COMMENT: 396866ab84f69995 130 ZfKpnab/q0P9gMwBv8gkPNMTTiY figure 6c ------- COMMENT: 396866ab84f69995 132 ZfKpnab/q0P9gMwBv8gkPNMTTiY figure 6c ------- COMMENT: 396866ab84f69995 134 ZfKpnab/q0P9gMwBv8gkPNMTTiY figure 6c ------- COMMENT: 396866ab84f69995 135 bTHAeFUNdbDUn62tiY2Y1EegANE figure S7A ------- COMMENT: 396866ab84f69995 136 bTHAeFUNdbDUn62tiY2Y1EegANE figure S7A ------- COMMENT: 396866ab84f69995 137 bTHAeFUNdbDUn62tiY2Y1EegANE figure S7A ------- COMMENT: 396866ab84f69995 138 bTHAeFUNdbDUn62tiY2Y1EegANE figure S7A ------- COMMENT: 396866ab84f69995 148 boDplkQKPZoOp7YmTQnrEdGh310 ((comment: Rescued to WT level) Rad21 phosphorylation level in cohesin hinge cs mutants is rescued by Δwpl1, while the loss of the Rad21 protein level in cohesin hinge ts mutants cannot be rescued by Δwpl1 ------- COMMENT: 396866ab84f69995 155 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: 396866ab84f69995 156 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: 396866ab84f69995 157 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: 396866ab84f69995 158 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: 396866ab84f69995 159 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: 396866ab84f69995 160 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: 396866ab84f69995 161 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: 396866ab84f69995 162 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: 396866ab84f69995 163 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: 396866ab84f69995 164 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: 396866ab84f69995 165 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: 396866ab84f69995 166 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: 396866ab84f69995 167 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: 396866ab84f69995 168 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: 396866ab84f69995 169 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: 396866ab84f69995 170 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: 396866ab84f69995 171 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: 396866ab84f69995 172 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: 396866ab84f69995 173 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: 396866ab84f69995 174 islrucHx9huQK3ul0duymUg80+E figure 1e ------- COMMENT: 396866ab84f69995 175 islrucHx9huQK3ul0duymUg80+E figure 1e ------- COMMENT: 396866ab84f69995 176 islrucHx9huQK3ul0duymUg80+E figure 1e ------- COMMENT: 396866ab84f69995 177 BcMypYvwaLSjaZS/r7aYmOXmwYw figure2 ------- COMMENT: 396866ab84f69995 180 iBbX2sDkL6uH6Slmt1+LO2KaCk8 figure 2C ------- COMMENT: 396866ab84f69995 181 YZsCq2MX0wN/NlAjDNNErWBBd1w figure S3 ------- COMMENT: 396866ab84f69995 182 YZsCq2MX0wN/NlAjDNNErWBBd1w figure S3 ------- COMMENT: 396866ab84f69995 183 YZsCq2MX0wN/NlAjDNNErWBBd1w figure S3 ------- COMMENT: 396866ab84f69995 184 YZsCq2MX0wN/NlAjDNNErWBBd1w figure S3 ------- COMMENT: 396866ab84f69995 185 YZsCq2MX0wN/NlAjDNNErWBBd1w figure S3 ------- COMMENT: 396866ab84f69995 186 YZsCq2MX0wN/NlAjDNNErWBBd1w figure S3 ------- COMMENT: 396866ab84f69995 187 YZsCq2MX0wN/NlAjDNNErWBBd1w figure S3 ------- COMMENT: 396866ab84f69995 188 YZsCq2MX0wN/NlAjDNNErWBBd1w figure S3 ------- COMMENT: 396866ab84f69995 189 YZsCq2MX0wN/NlAjDNNErWBBd1w figure S3 ------- COMMENT: 396866ab84f69995 190 YZsCq2MX0wN/NlAjDNNErWBBd1w figure S3 ------- COMMENT: 396866ab84f69995 191 YZsCq2MX0wN/NlAjDNNErWBBd1w figure S3 ------- COMMENT: 396866ab84f69995 192 YZsCq2MX0wN/NlAjDNNErWBBd1w figure S3 ------- COMMENT: 396866ab84f69995 193 YZsCq2MX0wN/NlAjDNNErWBBd1w figure S3 ------- COMMENT: 396866ab84f69995 194 YZsCq2MX0wN/NlAjDNNErWBBd1w figure S3 ------- COMMENT: 396866ab84f69995 195 YZsCq2MX0wN/NlAjDNNErWBBd1w figure S3 ------- COMMENT: 396866ab84f69995 196 YZsCq2MX0wN/NlAjDNNErWBBd1w figure S3 ------- COMMENT: 396866ab84f69995 197 YZsCq2MX0wN/NlAjDNNErWBBd1w figure S3 ------- COMMENT: 396866ab84f69995 198 YZsCq2MX0wN/NlAjDNNErWBBd1w figure S3 ------- COMMENT: 396866ab84f69995 199 YZsCq2MX0wN/NlAjDNNErWBBd1w figure S3 ------- COMMENT: 396866ab84f69995 200 YZsCq2MX0wN/NlAjDNNErWBBd1w figure S3 ------- COMMENT: 396866ab84f69995 201 YZsCq2MX0wN/NlAjDNNErWBBd1w figure S3 ------- COMMENT: 396866ab84f69995 202 SidOeVRd1nti76XJCMeKiOVxfy0 figure S4 ------- COMMENT: 396866ab84f69995 203 SidOeVRd1nti76XJCMeKiOVxfy0 figure S4 ------- COMMENT: 396866ab84f69995 204 SidOeVRd1nti76XJCMeKiOVxfy0 figure S4 ------- COMMENT: 396866ab84f69995 205 kAFVhHI5x0BwVja+A0ghrgYmVkc fig 3B ------- COMMENT: 396866ab84f69995 206 kAFVhHI5x0BwVja+A0ghrgYmVkc fig 3B ------- COMMENT: 396866ab84f69995 207 kAFVhHI5x0BwVja+A0ghrgYmVkc fig 3B ------- COMMENT: 396866ab84f69995 208 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1C ------- COMMENT: 396866ab84f69995 209 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 396866ab84f69995 210 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 396866ab84f69995 211 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 396866ab84f69995 212 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 396866ab84f69995 213 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 396866ab84f69995 214 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 396866ab84f69995 215 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 396866ab84f69995 216 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 396866ab84f69995 217 m9PuQZMN4JgcGJV1KB/6bsVsuIQ figure 3F ------- COMMENT: 396866ab84f69995 218 ATjZ6b1vXAMxdW5L4Ud+kK7TDk8 figure 4 AB ------- COMMENT: 396866ab84f69995 219 ATjZ6b1vXAMxdW5L4Ud+kK7TDk8 figure 4 AB ------- COMMENT: 396866ab84f69995 220 ATjZ6b1vXAMxdW5L4Ud+kK7TDk8 figure 4 AB ------- COMMENT: 396866ab84f69995 221 ATjZ6b1vXAMxdW5L4Ud+kK7TDk8 figure 4 AB ------- COMMENT: 396866ab84f69995 222 ATjZ6b1vXAMxdW5L4Ud+kK7TDk8 figure 4 AB ------- COMMENT: 396866ab84f69995 223 lVi4xwkr5Tsf/3UCIT20ySGuDes figure 4D-H ------- COMMENT: 396866ab84f69995 224 lVi4xwkr5Tsf/3UCIT20ySGuDes figure 4D-H ------- COMMENT: 396866ab84f69995 225 lVi4xwkr5Tsf/3UCIT20ySGuDes figure 4D-H ------- COMMENT: 396866ab84f69995 226 TxHq4b+49EvuxWZpdrMxFj1Nkws figure 5C ------- COMMENT: 396866ab84f69995 227 wA2zDi2ljZyRdZxkVyg7LW8joIY figure 5C ------- COMMENT: 396866ab84f69995 228 TxHq4b+49EvuxWZpdrMxFj1Nkws figure 5C ------- COMMENT: 396866ab84f69995 249 kwJYaMp8UU1UCc+JQEwtt2rfORA figure S8 ------- COMMENT: 396866ab84f69995 250 kwJYaMp8UU1UCc+JQEwtt2rfORA figure S8 ------- COMMENT: 397b85fc97c32b6a 1 SFNg5eFGDSgqIR9M63dJG+bP9sI We found that mNG-Cam1 could not local- ize to the SPB marked by Sad1-mCherry in pcp1-14 cells at the restrictive temperature (Figure 4D). ------- COMMENT: 397b85fc97c32b6a 2 aen/KQeIxEF/39wlx/O/pnhizXE Importantly, we also found that Pcp1-GFP could not localize to SPBs marked by Sad1-mCherry in cam1-E14 cells at the restrictive temperature (Figure 4E), suggest-ing that the PACT domain-Cam1 module directs SPB targeting. ------- COMMENT: 397b85fc97c32b6a 3 ZzLSCsvQsI0u8ag9ShJZC1H8fY8 Further evidence that Ppc89 and Pcp1 are closely associated was obtained by using a one-step purification of Ppc89-TAP, coupled with mass spec- trometry (MS) analysis. Pcp1 was identified as the most abundant copurifying protein (Supplemental Table S1). ------- COMMENT: 397b85fc97c32b6a 4 IhAcHAsmYxKERtSV68arn0/IB5I Ppc89 and Ppc89 M+C expressing cells exhibited the enlarged SPB phenotype (Figure 1D), as ob- served previously for FL Ppc89 (Rosenberg et al., 2006). ------- COMMENT: 397b85fc97c32b6a 5 NcMCx5SwuYfkQiI42EAJ+bIUHOE (Figure 1B) ------- COMMENT: 397b85fc97c32b6a 7 CqUCbfbbK6iC/MUICBHhSDpMzuw Pcp1 accumu- lated in enlarged Ppc89-containing structures upon overproduc- tion of FL Ppc89 (Figure 3). ------- COMMENT: 397b85fc97c32b6a 15 moLHrn+a2NcC9ArnAyWw9s13m/4 In contrast, Ppc89 N+M expressing cells displayed multiple foci of endogenous Ppc89-GFP (Figure 1D). ------- COMMENT: 397b85fc97c32b6a 16 NcMCx5SwuYfkQiI42EAJ+bIUHOE (Figure 1B) ------- COMMENT: 397b85fc97c32b6a 17 NcMCx5SwuYfkQiI42EAJ+bIUHOE (Figure 1B) ------- COMMENT: 397b85fc97c32b6a 18 IhAcHAsmYxKERtSV68arn0/IB5I Ppc89 and Ppc89 M+C expressing cells exhibited the enlarged SPB phenotype (Figure 1D), as ob- served previously for FL Ppc89 (Rosenberg et al., 2006). ------- COMMENT: 397b85fc97c32b6a 19 +GP/APBkntRvUU63kwkKtXj/pRY The Sid4 C-terminus interacts with the Ppc89 C-terminus (Rosenberg et al., 2006). Sid4- mCherry colocalized with endogenous Ppc89-GFP when FL or M+C were overex- pressed, both forming enlarged SPBs (Figure 2). This is consistent with the idea that as additional Ppc89 C-termini becomes available at the SPB, more Sid4 can interact and localize there. ------- COMMENT: 397b85fc97c32b6a 20 Es8/CKKejQ3p0VVEOsVWfg2FfO4 Similarly, we would expect overexpressed GFP-M+C to bind endoge- nous Pcp89 at the SPB to form larger SPBs and this was also observed (Supplemental Figure S1B). ------- COMMENT: 397b85fc97c32b6a 22 RAP9KoR3mCx96WV0RS1Yk1fwMfE When untagged Ppc89 N+M was overproduced, Sid4 was lost from the SPBs of cells that formed multiple Ppc89 foci (Figure 2). This result is consistent with the idea that N+M acts as a dominant nega- tive, outcompeting endogenous Ppc89 at SPBs and diluting the Sid4-mCherry signal to such an extent that it can no longer be detected. ------- COMMENT: 397b85fc97c32b6a 23 AVf+gFFzvrne4uE4s06rg+0hHlc Like GFP-Pcp1 expressed from the weak nmt81 promoter, the GFP-PACT domain alone colocalized with Ppc89-mCherry (Figure 4B). Thus, Pcp1’s PACT domain is sufficient for SPB targeting. ------- COMMENT: 397b85fc97c32b6a 24 jo42g/s+nT1bfgvGOA7xbG44T94 GFP-Ppc89(261-550) localized to SPBs marked by Sad1-mCherry, ------- COMMENT: 397b85fc97c32b6a 25 cZ2fTrlNidgK7rbzbOiY49wBzlA but GFP-Ppc89(267-550-K299A) was diffuse in the cytoplasm and nucleus or present in foci that did not overlap with the SPB (Figure 6C). ------- COMMENT: 397b85fc97c32b6a 26 oXarBzdyROoHPYRiu37ADh7oufk Additionally, while GFP-Pcp1(PACT) localized at the SPB, GFP-Pcp1(PACT-I1151A) was diffuse throughout the cy- tosol and nucleus (Figure 6D) ------- COMMENT: 39a5a6a1083e818a 4 bUWiUyXECscyYA3zPWv9aIBaChY inhibition by CCCP and DCCD ------- COMMENT: 39d20d8889ee33d4 9 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: 39d20d8889ee33d4 10 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: 39d20d8889ee33d4 11 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: 39d20d8889ee33d4 12 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: 39d20d8889ee33d4 14 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: 39df96dcfbe5d5ee 2 7de1SdZaK7LieX+/F7XR+r2jn0M We have previously demonstrated that Tas3 binds directly to Chp1 (Petrie et al., 2005). In addition, Tas3 binds to Ago1 and this interaction is independent of Chp1 (Figure 1A). Thus, Tas3 forms a bridge linking the Chp1 chromodomain protein to Ago1. ------- COMMENT: 39df96dcfbe5d5ee 8 nsjvbhbcKUqCx0IfI5PgXXnrn4I (dg repeat) Centromeric transcripts were only marginally elevated in tas3WG cells, in marked contrast to the 10- to 20-fold accumulation of transcripts in tas3-, dcr1-, or ago1-null cells (Figure 2B). ------- COMMENT: 39df96dcfbe5d5ee 9 F+k0A8aBM4gsevVeps6WWvybaFk Consistent with these findings, tas3WG mutant cells showed no defects in chromosome segregation (Table S2). ------- COMMENT: 39df96dcfbe5d5ee 10 O+mzrHu37TIzPl7Cyy9lau6Ukr8 Chromatin immunoprecipitation (ChIP) analyses showed that Ago1 was indeed localized at centromeres in the tas3WG mutant, a ------- COMMENT: 39df96dcfbe5d5ee 11 4xRZjCy9oj1onEgGs6qqgmo2ZX8 Further, centromeric siRNAs were similarly abundant in tas3WG and tas3+ cells (Figure 2D). ------- COMMENT: 39df96dcfbe5d5ee 12 2rrMVEZ6Y0GlLZSKuivgrA9wS58 ......as were Chp1 and the mutant Tas3WG protein (Figure 2C). ------- COMMENT: 39df96dcfbe5d5ee 13 2rrMVEZ6Y0GlLZSKuivgrA9wS58 ......as were Chp1 and the mutant Tas3WG protein (Figure 2C). ------- COMMENT: 39df96dcfbe5d5ee 14 08omL+gLGQIEalevs2xr0rscZi4 F276A-ago1 (Figure S3B) caused a slight defect in silencing of the dg cen::ura4+ reporter (Figure S3C). ------- COMMENT: 39df96dcfbe5d5ee 15 x0DL/uruH0a3qrAamgzKkLwqtgY the double mutant (tas3WG, F276Aago1) displayed markedly elevated levels of total centromeric transcripts (Figure 2E), similar to an ago1 null. ------- COMMENT: 39df96dcfbe5d5ee 16 bs0baUwGXp+xe0Arkk1X8Y319dk centromeric siRNAs were present in the F276Aago1 and tas3WG single mutants but were undetectable in the double mutant (Figure 2F). ------- COMMENT: 39df96dcfbe5d5ee 17 bs0baUwGXp+xe0Arkk1X8Y319dk centromeric siRNAs were present in the F276Aago1 and tas3WG single mutants but were undetectable in the double mutant (Figure 2F). ------- COMMENT: 39df96dcfbe5d5ee 18 y5zzvvV21eL+sFbptnBapPPW5Jc The reestablishmentof centromeric heterochromatin was then monitored after reintroduction of clr4+. Addition of clr4+to tas3+ cells allowed efficient establishment of heterochromatinand silencing of the cen::ura4+ transgene atdg (Figure 3A). In marked contrast, on reintroduction ofclr4+, cells expressing tas3WG failed to silence the cen::ura4+ reporter ------- COMMENT: 39df96dcfbe5d5ee 19 mi2apXToiufjjG1Xtm/9yhvAsRY Tas3WG Cells Cannot Efficiently Establish De Novo Centromeric Heterochromatin ------- COMMENT: 39df96dcfbe5d5ee 20 mi2apXToiufjjG1Xtm/9yhvAsRY Tas3WG Cells Cannot Efficiently Establish De Novo Centromeric Heterochromatin ------- COMMENT: 39e9205957ce5637 4 IC7ef3sfVwCaVgOvaXuL9Kz+czI (comment: git2-1 is effectively null, even though it isn't a complete deletion of the coding sequence) ------- COMMENT: 39e9205957ce5637 18 IC7ef3sfVwCaVgOvaXuL9Kz+czI (comment: git2-1 is effectively null, even though it isn't a complete deletion of the coding sequence) ------- COMMENT: 39e9205957ce5637 20 wUweQoOJ0BGr6pvSK5fGbxDtzco (comment: glycerol = derepressing for glucose repression) (comment: also assayed using lacZ under fbp1 promoter and maltose carbon source, also derepressing) ------- COMMENT: 39e9205957ce5637 22 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 27 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 34 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter ------- COMMENT: 39e9205957ce5637 35 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 36 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 37 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 38 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 39 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 40 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 41 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 42 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 43 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 44 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 45 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 47 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 49 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 61 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 62 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 63 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 64 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 65 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 66 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 67 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 39e9205957ce5637 68 y3EPT0xlKIzD2sRptbuA0nrbepE (comment: also assayed using lacZ under fbp1 promoter) ------- COMMENT: 3a02661c59ca34de 19 2PgFnL2DO9EPyL5UIxRKNJHmtNM (comment: maybe not shown strongly in this paper but I'm trying to get the git genes annotated to this term because pka1 phosphorylates rst2 which excludes rst2 from the nucleus. rst2 when in the nucleus activates ste11 transcription.) ------- COMMENT: 3a02661c59ca34de 20 2PgFnL2DO9EPyL5UIxRKNJHmtNM (comment: maybe not shown strongly in this paper but I'm trying to get the git genes annotated to this term because pka1 phosphorylates rst2 which excludes rst2 from the nucleus. rst2 when in the nucleus activates ste11 transcription.) ------- COMMENT: 3a1acc53ad7cd3a5 27 4yCVBSkl6bg2aTrDdCZ1ZS1USlw fig 5b ------- COMMENT: 3a1acc53ad7cd3a5 28 4yCVBSkl6bg2aTrDdCZ1ZS1USlw fig 5b ------- COMMENT: 3a1acc53ad7cd3a5 35 CXtOEaqMu3AHSp0HMhEV+QbL8iE phosphorylation of rad9 by DDK releases rad9 from damaged chromatin and allows repair factors to come in... (Figure 6) ------- COMMENT: 3a1acc53ad7cd3a5 36 CXtOEaqMu3AHSp0HMhEV+QbL8iE phosphorylation of rad9 by DDK releases rad9 from damaged chromatin and allows repair factors to come in... (Figure 6) ------- COMMENT: 3a1acc53ad7cd3a5 39 CXtOEaqMu3AHSp0HMhEV+QbL8iE phosphorylation of rad9 by DDK releases rad9 from damaged chromatin and allows repair factors to come in... (Figure 6) ------- COMMENT: 3a3a0d9e76ed714d 4 6vWgGg1/21FHwCmntXnIWP86eic Therefore, we propose that a major role of Atg43 in the mitophagy process is to tether Atg8 to mitochondria through direct interaction with Atg8 via the AIM region. ------- COMMENT: 3a3a0d9e76ed714d 6 jcW3Opjwx+7/JjpqQWPLsnwKSDI Figure 2F, 2G, 2H ------- COMMENT: 3a3a0d9e76ed714d 7 oXrahwH/EUTq3pU54FPDXMsc++Y Figure 1 ------- COMMENT: 3a3a0d9e76ed714d 8 RsdBr2g6Li7NFgcnnDaZXk0X+T8 Figure 1D ------- COMMENT: 3a3a0d9e76ed714d 9 ernVuLQ3ugzbI6GXr4Wb6RsgMe4 Figure 2A ------- COMMENT: 3a3a0d9e76ed714d 12 TXb8hlDFjDDlCmOgh+WV9ZJDMk8 Fig- ure 5—figure supplement 1F ------- COMMENT: 3a3a0d9e76ed714d 13 yULAV+8NqEK9cnAogvDND9AYYF0 In the absence of Mim1 or Mim2, the GFP-Atg43 signal at the mitochondria was severely decreased (Figure 5C) ------- COMMENT: 3a3a0d9e76ed714d 14 ldzAi8Clt1B1vuJAg5Grwu5iZTQ Consistent with this, mitophagy was impaired in the mim1D and mim2D mutants (Figure 5D) ------- COMMENT: 3a3a0d9e76ed714d 20 yULAV+8NqEK9cnAogvDND9AYYF0 In the absence of Mim1 or Mim2, the GFP-Atg43 signal at the mitochondria was severely decreased (Figure 5C) ------- COMMENT: 3a3a0d9e76ed714d 21 TXb8hlDFjDDlCmOgh+WV9ZJDMk8 Fig- ure 5—figure supplement 1F ------- COMMENT: 3a3a0d9e76ed714d 22 ldzAi8Clt1B1vuJAg5Grwu5iZTQ Consistent with this, mitophagy was impaired in the mim1D and mim2D mutants (Figure 5D) ------- COMMENT: 3a3a0d9e76ed714d 25 WIQobKVmOSwF4pnw3zyHiiYk2Lk Figure 1A ------- COMMENT: 3a3a0d9e76ed714d 28 oXrahwH/EUTq3pU54FPDXMsc++Y Figure 1 ------- COMMENT: 3a3a0d9e76ed714d 29 oXrahwH/EUTq3pU54FPDXMsc++Y Figure 1 ------- COMMENT: 3a3a0d9e76ed714d 31 uFC42l858qGYH9j5iNDyochZJeU Figure 2 ------- COMMENT: 3a3a0d9e76ed714d 33 yyMNf1x4ofpAA8TPiE7bhUbV4SU Figure 3B ------- COMMENT: 3a3a0d9e76ed714d 34 ZwIii3D7SK0c1aAu17XfPupbrHw Figure 3B/4B ------- COMMENT: 3a3a0d9e76ed714d 35 ZnAIjK5cSs4bZZz107dPWRzG8gw (comment: CHECK check genotype******)Figure 3C ------- COMMENT: 3a3a0d9e76ed714d 36 WR4eY9sTthO3PnzjKXFbZFO6hx0 Figure 3D ------- COMMENT: 3a3a0d9e76ed714d 37 WR4eY9sTthO3PnzjKXFbZFO6hx0 Figure 3D ------- COMMENT: 3a3a0d9e76ed714d 38 WR4eY9sTthO3PnzjKXFbZFO6hx0 Figure 3D ------- COMMENT: 3a3a0d9e76ed714d 40 EiogtJrHmvE8aL3L4uZUfidbzCo Figure 3H ------- COMMENT: 3a3a0d9e76ed714d 41 EiogtJrHmvE8aL3L4uZUfidbzCo Figure 3H ------- COMMENT: 3a3a0d9e76ed714d 42 EiogtJrHmvE8aL3L4uZUfidbzCo Figure 3H ------- COMMENT: 3a3a0d9e76ed714d 43 lPPITs++El30fo52lrFYe9ROwgQ Figure 3I ------- COMMENT: 3a3a0d9e76ed714d 44 V4/1fDNpkBdp6etjvP3FkZvRpIQ Figure 3I ------- COMMENT: 3a3a0d9e76ed714d 45 7xK4kKNVzUW/0ir6MPjAbUtJFLc Figure 3K ------- COMMENT: 3a3a0d9e76ed714d 46 WI5tAkcerjyhlsXweoj8DX9nnxU Figure 4D ------- COMMENT: 3a3a0d9e76ed714d 47 WI5tAkcerjyhlsXweoj8DX9nnxU Figure 4D ------- COMMENT: 3a3a0d9e76ed714d 48 +lGokglJTKvk0vvTDRIpieifdEo Figure 4B ------- COMMENT: 3a3a0d9e76ed714d 51 yJWA/NmF4RYPbwESOlkyoLFCMpo Figure 3 Supp 1B&C ------- COMMENT: 3a3a0d9e76ed714d 52 l2VUj622VPhe7/kCu8uZCLAZu94 whereas Atg43 with a truncation of the 60 C-terminal aa was defective in mitophagy (Figure 4B) ------- COMMENT: 3a3a0d9e76ed714d 53 /xLedwQIQPWh9cHWaE8Fi2XNRBg figure 4B Atg43 lacking the 20 C-terminal aa exhibited only a partial defect in mitophagy, ------- COMMENT: 3a3a0d9e76ed714d 54 s7cvhfiYiveaAA2KzWLwMqq+fAs Figure 4C ------- COMMENT: 3a3a0d9e76ed714d 57 VYQHtJ8BDDdMavuDO0ebs6Q0JKo Figure 4G ------- COMMENT: 3a3a0d9e76ed714d 58 bKP4hXPl+56EqMFibW21tH+QCJU The interaction between full-length Atg43 and Mim2 was confirmed using reciprocal immunoprecipitation experi- ments (Figure 5A and Figure 5—figure supplement 1B). ------- COMMENT: 3a3a0d9e76ed714d 60 DILtIiLNM/EgDLcJx1eRptpT8RQ ------- COMMENT: 3a3a0d9e76ed714d 67 hcC369Q8qGWB2LiSqN1nCcZ07G0 This raises the possibility that the MIM complex assists Atg43 through facilitating its mitochondrial localization. ------- COMMENT: 3a3a0d9e76ed714d 68 hcC369Q8qGWB2LiSqN1nCcZ07G0 This raises the possibility that the MIM complex assists Atg43 through facilitating its mitochondrial localization. ------- COMMENT: 3a3a0d9e76ed714d 69 mVCS251j6QFwjKTpGUr3WF7V8sQ We confirmed that Mim1 and Mim2 are required for stable localization of Tom70 on mitochondria in fis- sion yeast (Figure 5—figure supplement 1H ------- COMMENT: 3a3a0d9e76ed714d 70 mVCS251j6QFwjKTpGUr3WF7V8sQ We confirmed that Mim1 and Mim2 are required for stable localization of Tom70 on mitochondria in fis- sion yeast (Figure 5—figure supplement 1H ------- COMMENT: 3a3a0d9e76ed714d 71 ZCnf1Wrk6b7/c7gEl+ilkSpyYrM Atg43 was observed on mitochondria in the absence of Tom70 (Figure 5E) and vice versa (Figure 5—figure supplement 1I) ------- COMMENT: 3a3a0d9e76ed714d 72 ZCnf1Wrk6b7/c7gEl+ilkSpyYrM Atg43 was observed on mitochondria in the absence of Tom70 (Figure 5E) and vice versa (Figure 5—figure supplement 1I) ------- COMMENT: 3a3a0d9e76ed714d 73 ZCnf1Wrk6b7/c7gEl+ilkSpyYrM Atg43 was observed on mitochondria in the absence of Tom70 (Figure 5E) and vice versa (Figure 5—figure supplement 1I) ------- COMMENT: 3a3a0d9e76ed714d 74 ZCnf1Wrk6b7/c7gEl+ilkSpyYrM Atg43 was observed on mitochondria in the absence of Tom70 (Figure 5E) and vice versa (Figure 5—figure supplement 1I) ------- COMMENT: 3a3a0d9e76ed714d 75 ZCnf1Wrk6b7/c7gEl+ilkSpyYrM Atg43 was observed on mitochondria in the absence of Tom70 (Figure 5E) and vice versa (Figure 5—figure supplement 1I) ------- COMMENT: 3a3a0d9e76ed714d 76 ZCnf1Wrk6b7/c7gEl+ilkSpyYrM Atg43 was observed on mitochondria in the absence of Tom70 (Figure 5E) and vice versa (Figure 5—figure supplement 1I) ------- COMMENT: 3a3a0d9e76ed714d 77 ZCnf1Wrk6b7/c7gEl+ilkSpyYrM Atg43 was observed on mitochondria in the absence of Tom70 (Figure 5E) and vice versa (Figure 5—figure supplement 1I) ------- COMMENT: 3a3a0d9e76ed714d 78 ZCnf1Wrk6b7/c7gEl+ilkSpyYrM Atg43 was observed on mitochondria in the absence of Tom70 (Figure 5E) and vice versa (Figure 5—figure supplement 1I) ------- COMMENT: 3a3a0d9e76ed714d 79 hcC369Q8qGWB2LiSqN1nCcZ07G0 This raises the possibility that the MIM complex assists Atg43 through facilitating its mitochondrial localization. ------- COMMENT: 3a3a0d9e76ed714d 80 hcC369Q8qGWB2LiSqN1nCcZ07G0 This raises the possibility that the MIM complex assists Atg43 through facilitating its mitochondrial localization. ------- COMMENT: 3a3a0d9e76ed714d 81 84kPGn4trDgFEiGGEszUUY7jjUg Figure 6B,D ------- COMMENT: 3a3a0d9e76ed714d 82 ITEsfgBVxxN1vS8jspJUWdH2f5E Figure 6B ------- COMMENT: 3a3a0d9e76ed714d 83 ITEsfgBVxxN1vS8jspJUWdH2f5E Figure 6B ------- COMMENT: 3a3a0d9e76ed714d 87 9Z34Ksmbs7m9qf0aDQnyBxSJvx0 By contrast, the atg7D and atg43DAIM mutants did not exhibit such an increased in superoxide (Figure 7D) ------- COMMENT: 3a3a0d9e76ed714d 88 9Z34Ksmbs7m9qf0aDQnyBxSJvx0 By contrast, the atg7D and atg43DAIM mutants did not exhibit such an increased in superoxide (Figure 7D) ------- COMMENT: 3a5f16c5574b0a11 22 Y2R+bQ8C8W/ICQNmAYw9ov99B8Y (comment: Kenichi comment mitotic defects mitotic defects caused by ace2 deletion) ------- COMMENT: 3a5f16c5574b0a11 23 j8PKGKsg1BJthKYvbVmM68JPDgY (comment: Kenichi comment mitotic defects mitotic defects caused by eng1 deletion) ------- COMMENT: 3a5f16c5574b0a11 24 Ubu6P7IpEhLlA56Tt5RnrIptLl4 (comment: Kenichi comment mitotic defects mitotic defects caused by 343.20 deletion) ------- COMMENT: 3a8dc608ff891ebb 1 ltWqzsVMpjaOGPwKzXyh+5/c1MA (comment: especially during S and G2 phases) ------- COMMENT: 3a8dc608ff891ebb 2 ltWqzsVMpjaOGPwKzXyh+5/c1MA (comment: especially during S and G2 phases) ------- COMMENT: 3a8dc608ff891ebb 3 ltWqzsVMpjaOGPwKzXyh+5/c1MA (comment: especially during S and G2 phases) ------- COMMENT: 3a8dc608ff891ebb 4 ltWqzsVMpjaOGPwKzXyh+5/c1MA (comment: especially during S and G2 phases) ------- COMMENT: 3a957560e28cf7a5 50 cjC/U4JEzkjZf/lc9VyNYRIr92k (comment: distal to break point) ------- COMMENT: 3a957560e28cf7a5 77 DMZ9Fh2oMFtIHb22gvzseGqH6fQ (comment: same as without htb1-K119R) ------- COMMENT: 3a957560e28cf7a5 78 qGYdgjFKX0ZCFfSZHL3oqDvmA4Q (comment: same as without csn1delta) ------- COMMENT: 3a957560e28cf7a5 79 X22EuduQ3OuA94Ev6ee4PVbA9+8 (comment: same as without csn5delt) ------- COMMENT: 3a957560e28cf7a5 80 Hmh8DpXflwX7KcCISSJkhZFG6fg (comment: worse than without rqh1delta) ------- COMMENT: 3a957560e28cf7a5 86 ThYcfXHr9BuGF2KtviZpoXd6kYc (comment: same as rhp6delta alone) ------- COMMENT: 3a957560e28cf7a5 87 ThYcfXHr9BuGF2KtviZpoXd6kYc (comment: same as rhp6delta alone) ------- COMMENT: 3a957560e28cf7a5 92 toUNQSOH69sHYn9xpufl5Ap4pAY (comment: same as without exo1delta) ------- COMMENT: 3a957560e28cf7a5 93 toUNQSOH69sHYn9xpufl5Ap4pAY (comment: same as without exo1delta) ------- COMMENT: 3a957560e28cf7a5 95 VonpSehJt6x+1GE84WefXArk/sk (comment: same as without exo1+ overexpression) ------- COMMENT: 3a995b73ca205826 1 ZzhPzOWYdw1AeZynj00fckkKg3o Our data indicates that Rng3p is required to establish active Myo2p motors.The control experiments (where cells were shifted to 37uC at 22hours post-induction) indicated that Rng3p was not essential formaintaining Myo2p motility once an active population of motorshad been synthesized, as previously reported [24]...........Collectively our findings suggest that Rng3p is required to generate an active and stable population of Myo2p motors. ------- COMMENT: 3a995b73ca205826 2 +cYJsddaKLxluxC/VItnf2GqpvU However, most filaments (99%) bound by -E1 were non-motile, while most filaments bound by wild-type Myo2p were motile (Movie S2). ------- COMMENT: 3a995b73ca205826 3 N7A3GKNU3EX2/K1lur42NgpnInQ (comment: CHECK atpase activity assay) However, -E1 motors exhibited relatively low activity under either condition (Figure S1). These experiments performed in the absence of actin suggest that defects in -E1 motors are not specific to actin displacement and motility, and probably reflect a general defect in conformation and function. ------- COMMENT: 3a995b73ca205826 5 agAYctuOF9GA+xTc4EdEQDPxwZ0 However, the lower molecular weight form of -E1 failed to accumulate over time following protease addition (Figure 4C), suggesting an altered conformation more sensitive to proteolysis. ------- COMMENT: 3aad414b8b97983b 16 /mr5W8J6IrjZQFfK18fOLDImgxs (comment: assayed using SV40 NLS-GFP-LacZ reporter protein) ------- COMMENT: 3aad414b8b97983b 37 /mr5W8J6IrjZQFfK18fOLDImgxs (comment: assayed using SV40 NLS-GFP-LacZ reporter protein) ------- COMMENT: 3aad414b8b97983b 41 jOTLRpW8LPghfukdLYuPnbYLAis (comment: same as rae1-167 single mutant) ------- COMMENT: 3aad414b8b97983b 42 jOTLRpW8LPghfukdLYuPnbYLAis (comment: same as rae1-167 single mutant) ------- COMMENT: 3adb4fd16e0b2a9a 21 jw3HwxenrMH5G8/ORRhNDb7AWfc (comment: CHECK assayed using bub1) ------- COMMENT: 3ae02f9ef40a096c 4 cbnqb6Vc3YZxFdyXshT12wf7XNs ------- COMMENT: 3ae02f9ef40a096c 6 cbnqb6Vc3YZxFdyXshT12wf7XNs ------- COMMENT: 3ae02f9ef40a096c 7 cbnqb6Vc3YZxFdyXshT12wf7XNs ------- COMMENT: 3ae02f9ef40a096c 9 cbnqb6Vc3YZxFdyXshT12wf7XNs ------- COMMENT: 3ae02f9ef40a096c 19 cbnqb6Vc3YZxFdyXshT12wf7XNs ------- COMMENT: 3ae02f9ef40a096c 44 cbnqb6Vc3YZxFdyXshT12wf7XNs ------- COMMENT: 3b1166bb2423f4aa 10 f6gs9XjNj6W0UW4aFuAZgr8TT8M Additionally, we established that the loss of the HMG-domain of Lsd1 does not affect the interaction between Lsd1 and Phf1 (Fig 1D) or Phf2 (Fig 1E), ------- COMMENT: 3b1166bb2423f4aa 11 sP9VigM9eq3s7+vEmMViT+7r9I4 We also observed that the loss of the C-terminus of Lsd2 does not disrupt the interactions between Lsd2 and Phf1 (Fig 1F) or Phf2 (Fig 1G). ------- COMMENT: 3b1166bb2423f4aa 12 MhPU2cQ+YUdUYbaSrldTV3dfyMY Notably, the localizations of Lsd1 and Lsd2 are diminished at the pro- moter regions in the absence of a functional C-terminus (Fig 1C). This result demonstrates that the C-terminal domains of Lsd1 and Lsd2 are involved in their chromatin binding at these regions. ------- COMMENT: 3b1166bb2423f4aa 13 MhPU2cQ+YUdUYbaSrldTV3dfyMY Notably, the localizations of Lsd1 and Lsd2 are diminished at the pro- moter regions in the absence of a functional C-terminus (Fig 1C). This result demonstrates that the C-terminal domains of Lsd1 and Lsd2 are involved in their chromatin binding at these regions. ------- COMMENT: 3b1166bb2423f4aa 14 3Ml7fqIeqEN+IHKcuzenTSY9hag lsd1-ΔHMG set1Δ and lsd2-ΔC clr4Δ double mutants resulted in inviable daughter cells, suggesting that Lsd1 has essential overlapping functions with Set1, while Lsd2 has critical overlapping roles with Clr4 (Fig 2A). ------- COMMENT: 3b1166bb2423f4aa 15 3Ml7fqIeqEN+IHKcuzenTSY9hag lsd1-ΔHMG set1Δ and lsd2-ΔC clr4Δ double mutants resulted in inviable daughter cells, suggesting that Lsd1 has essential overlapping functions with Set1, while Lsd2 has critical overlapping roles with Clr4 (Fig 2A). ------- COMMENT: 3b1166bb2423f4aa 16 UoxHK7hDkS6QYafSqjNc/ynyzoQ that without Clr4, both Lsd1-FTP and Lsd2-FTP show an increase in protein levels, compared to the wild-type cells (Fig 2I). ------- COMMENT: 3b1166bb2423f4aa 18 jEsxzsP0yA/mNGC4U2nJRQvf8wg The western blot results suggest that the loss of any member of CLRC complex enhances the protein amount of Lsd1 (Fig 3C) or Lsd2 (Fig 3D). ------- COMMENT: 3b1166bb2423f4aa 19 jEsxzsP0yA/mNGC4U2nJRQvf8wg The western blot results suggest that the loss of any member of CLRC complex enhances the protein amount of Lsd1 (Fig 3C) or Lsd2 (Fig 3D). ------- COMMENT: 3b1166bb2423f4aa 20 jEsxzsP0yA/mNGC4U2nJRQvf8wg The western blot results suggest that the loss of any member of CLRC complex enhances the protein amount of Lsd1 (Fig 3C) or Lsd2 (Fig 3D). ------- COMMENT: 3b1166bb2423f4aa 21 f8KnazznTGPg3KhNCVofNyycqL0 Loss of Ddb1 slightly enhances the Lsd1-FTP level, while having no significant effect on the Lsd2-FTP level (Fig 3E). ------- COMMENT: 3b1166bb2423f4aa 22 UoxHK7hDkS6QYafSqjNc/ynyzoQ that without Clr4, both Lsd1-FTP and Lsd2-FTP show an increase in protein levels, compared to the wild-type cells (Fig 2I). ------- COMMENT: 3b1166bb2423f4aa 24 jEsxzsP0yA/mNGC4U2nJRQvf8wg The western blot results suggest that the loss of any member of CLRC complex enhances the protein amount of Lsd1 (Fig 3C) or Lsd2 (Fig 3D). ------- COMMENT: 3b1166bb2423f4aa 25 jEsxzsP0yA/mNGC4U2nJRQvf8wg The western blot results suggest that the loss of any member of CLRC complex enhances the protein amount of Lsd1 (Fig 3C) or Lsd2 (Fig 3D). ------- COMMENT: 3b1166bb2423f4aa 26 jEsxzsP0yA/mNGC4U2nJRQvf8wg The western blot results suggest that the loss of any member of CLRC complex enhances the protein amount of Lsd1 (Fig 3C) or Lsd2 (Fig 3D). ------- COMMENT: 3b1166bb2423f4aa 27 jFKZg4h97gtJJ1m+dzWF4kobLps In contrast, the protein levels of Lsd1-FTP and Lsd2-FTP show a notable decrease in the absence of Set1 (Fig 2I). ------- COMMENT: 3b1166bb2423f4aa 28 zdMt4HfYQ5y44McOhqp3EB0IuSY loss of most members of the COMPASS complex, including Set1, Spp1, Swd1, Swd3, Swd2, and Ash2 leads to a significantly decreased amount of Lsd1 protein (Fig 3A) ------- COMMENT: 3b1166bb2423f4aa 29 zdMt4HfYQ5y44McOhqp3EB0IuSY loss of most members of the COMPASS complex, including Set1, Spp1, Swd1, Swd3, Swd2, and Ash2 leads to a significantly decreased amount of Lsd1 protein (Fig 3A) ------- COMMENT: 3b1166bb2423f4aa 30 zdMt4HfYQ5y44McOhqp3EB0IuSY loss of most members of the COMPASS complex, including Set1, Spp1, Swd1, Swd3, Swd2, and Ash2 leads to a significantly decreased amount of Lsd1 protein (Fig 3A) ------- COMMENT: 3b1166bb2423f4aa 31 zdMt4HfYQ5y44McOhqp3EB0IuSY loss of most members of the COMPASS complex, including Set1, Spp1, Swd1, Swd3, Swd2, and Ash2 leads to a significantly decreased amount of Lsd1 protein (Fig 3A) ------- COMMENT: 3b1166bb2423f4aa 32 zdMt4HfYQ5y44McOhqp3EB0IuSY loss of most members of the COMPASS complex, including Set1, Spp1, Swd1, Swd3, Swd2, and Ash2 leads to a significantly decreased amount of Lsd1 protein (Fig 3A) ------- COMMENT: 3b1166bb2423f4aa 33 jFKZg4h97gtJJ1m+dzWF4kobLps In contrast, the protein levels of Lsd1-FTP and Lsd2-FTP show a notable decrease in the absence of Set1 (Fig 2I). ------- COMMENT: 3b1166bb2423f4aa 34 b35dGRa/xW/Bk1BHJS27RmaIhmw A similar pattern was observed in the Lsd2 samples, although the Lsd2 protein levels also decreased with shg1Δ (Fig 3B). ------- COMMENT: 3b1166bb2423f4aa 35 b35dGRa/xW/Bk1BHJS27RmaIhmw A similar pattern was observed in the Lsd2 samples, although the Lsd2 protein levels also decreased with shg1Δ (Fig 3B). ------- COMMENT: 3b1166bb2423f4aa 36 b35dGRa/xW/Bk1BHJS27RmaIhmw A similar pattern was observed in the Lsd2 samples, although the Lsd2 protein levels also decreased with shg1Δ (Fig 3B). ------- COMMENT: 3b1166bb2423f4aa 37 b35dGRa/xW/Bk1BHJS27RmaIhmw A similar pattern was observed in the Lsd2 samples, although the Lsd2 protein levels also decreased with shg1Δ (Fig 3B). ------- COMMENT: 3b1166bb2423f4aa 38 b35dGRa/xW/Bk1BHJS27RmaIhmw A similar pattern was observed in the Lsd2 samples, although the Lsd2 protein levels also decreased with shg1Δ (Fig 3B). ------- COMMENT: 3b1166bb2423f4aa 39 b35dGRa/xW/Bk1BHJS27RmaIhmw A similar pattern was observed in the Lsd2 samples, although the Lsd2 protein levels also decreased with shg1Δ (Fig 3B). ------- COMMENT: 3b1166bb2423f4aa 40 nA5yDViX79q/WdGQ3FR7AxEWnrU Lsd1 (Fig 4A) or Lsd2 (Fig 4B) was detected in the presence of a temperature-sensitive 26S pro- teasome subunit mutant, mts2-1, which inactivates the proteasome at 33 ̊C [111] and thereby stabilizes Lsd1 and Lsd2 (Fig 4A and 4B). ------- COMMENT: 3b1166bb2423f4aa 41 nA5yDViX79q/WdGQ3FR7AxEWnrU Lsd1 (Fig 4A) or Lsd2 (Fig 4B) was detected in the presence of a temperature-sensitive 26S pro- teasome subunit mutant, mts2-1, which inactivates the proteasome at 33 ̊C [111] and thereby stabilizes Lsd1 and Lsd2 (Fig 4A and 4B). ------- COMMENT: 3b1166bb2423f4aa 44 tQsb2FZS2b4xwH0+SLPrBKH7Ydo (Fig 5A) ------- COMMENT: 3b1166bb2423f4aa 45 RZQ9N7HNeV/o81gWru8PP4pf1qc We found that the loss of any members in CLRC promotes the protein levels of Set1, both at 30 ̊C or 37 ̊C (Fig 5A), indicating that the intact CLRC restricts the amount of Set1. ------- COMMENT: 3b1166bb2423f4aa 46 RZQ9N7HNeV/o81gWru8PP4pf1qc We found that the loss of any members in CLRC promotes the protein levels of Set1, both at 30 ̊C or 37 ̊C (Fig 5A), indicating that the intact CLRC restricts the amount of Set1. ------- COMMENT: 3b1166bb2423f4aa 47 RZQ9N7HNeV/o81gWru8PP4pf1qc We found that the loss of any members in CLRC promotes the protein levels of Set1, both at 30 ̊C or 37 ̊C (Fig 5A), indicating that the intact CLRC restricts the amount of Set1. ------- COMMENT: 3b1166bb2423f4aa 49 m0zUCtm6+kRlIOcj/qRslEsCt8g Both cul4-1 or ddb1Δ enhanced the amount of Set1 protein, indicating that both CLRC and CRL4 complexes modulate Set1 levels (Fig 5C) ------- COMMENT: 3b1166bb2423f4aa 50 OhnAZRGVvemY8+omA1X4DRKhopg Although no significant alterations were detected for Flag-Set1 protein levels between wild-type and clr4W31G cells at 30 ̊C, the elevated Flag-Set1 is less pronounced in clr4W31G compared to clr4Δ at 37 ̊C (Fig 5B), ------- COMMENT: 3b1166bb2423f4aa 51 m8deNRV4+WPMau6AWtRrWFHBWYs However, without Set1, Lsd1/2 protein levels are no longer upregulated during heat stress (Fig 4C and 4D), which implies that Set1 is required to elevate the protein levels of Lsd1 and Lsd2 under heat stress. ------- COMMENT: 3b1166bb2423f4aa 52 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 3b1166bb2423f4aa 53 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 3b1166bb2423f4aa 54 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 3b1166bb2423f4aa 55 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 3b1166bb2423f4aa 56 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 3b1166bb2423f4aa 57 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 3b1166bb2423f4aa 58 f2u1jVHykql6mfiJqJuKccUYcpk At 37 ̊C, only marginal changes in Lsd1 protein levels were observed in H3K4R and H2B-K119R mutants (Fig 6D). However, a significant reduction of Lsd2 was seen in both H3K4R and H2B K119R mutants (Fig 6E), ------- COMMENT: 3b1166bb2423f4aa 63 8FJQj+rfBadJAPQby9jT+wCL3MU Notably, deletion of the Lsd2 HMG-domain results in lethality akin to that in the complete loss of Lsd2, which further implies the unknown and important functions of the C-terminus of these two proteins ------- COMMENT: 3b1166bb2423f4aa 64 Nk2ESUNIoI4w2SB1kDTu+XL5gL0 Previous studies have shown that Lsd1/2 proteins bind to the promoters of a few hundred genes [70,73,74], suggesting that Lsd1/2 proteins are selectively recruited to those genes. Our ChIP-Seq analysis yields a highly similar set of genomic loci where Lsd1 and Lsd2 are enriched just upstream of the transcriptional start site (TSS) (Fig 1C). This result indicates that Lsd1 and Lsd2 mostly bind to the promoter region of genes and are likely to cooperate with other transcription factors that are involved in regulating gene expression. ------- COMMENT: 3b1166bb2423f4aa 65 Nk2ESUNIoI4w2SB1kDTu+XL5gL0 Previous studies have shown that Lsd1/2 proteins bind to the promoters of a few hundred genes [70,73,74], suggesting that Lsd1/2 proteins are selectively recruited to those genes. Our ChIP-Seq analysis yields a highly similar set of genomic loci where Lsd1 and Lsd2 are enriched just upstream of the transcriptional start site (TSS) (Fig 1C). This result indicates that Lsd1 and Lsd2 mostly bind to the promoter region of genes and are likely to cooperate with other transcription factors that are involved in regulating gene expression. ------- COMMENT: 3b1166bb2423f4aa 66 f6gs9XjNj6W0UW4aFuAZgr8TT8M Additionally, we established that the loss of the HMG-domain of Lsd1 does not affect the interaction between Lsd1 and Phf1 (Fig 1D) or Phf2 (Fig 1E), ------- COMMENT: 3b1166bb2423f4aa 67 sP9VigM9eq3s7+vEmMViT+7r9I4 We also observed that the loss of the C-terminus of Lsd2 does not disrupt the interactions between Lsd2 and Phf1 (Fig 1F) or Phf2 (Fig 1G). ------- COMMENT: 3b1166bb2423f4aa 68 zobAaZuxERRF7IV17k5z36MORfk The Lsd2-BD and Phf1-BD strains could self-activate the reporter genes without Gal4 activating domain, suggesting that Lsd2 and Phf1 alone could recruit basal transcriptional factors to initiate reporter gene transcription (S4 Fig). ------- COMMENT: 3b1166bb2423f4aa 69 zobAaZuxERRF7IV17k5z36MORfk The Lsd2-BD and Phf1-BD strains could self-activate the reporter genes without Gal4 activating domain, suggesting that Lsd2 and Phf1 alone could recruit basal transcriptional factors to initiate reporter gene transcription (S4 Fig). ------- COMMENT: 3b1166bb2423f4aa 70 AOb9rCJG/3hacR45xvq6hDBo8pI lsd1-ΔHMG clr4Δ cells are viable but sick, which implies a negative genetic interaction between Lsd1 and Clr4 (Figs 2A and S1B). ------- COMMENT: 3b1166bb2423f4aa 71 av/4PIsF9E27L33/Zseu5eCELxY The triple mutants lsd1- ΔHMG set1Δ clr4Δ and lsd2-ΔC set1Δ clr4Δ are lethal (S1D Fig). ------- COMMENT: 3b1166bb2423f4aa 72 av/4PIsF9E27L33/Zseu5eCELxY The triple mutants lsd1- ΔHMG set1Δ clr4Δ and lsd2-ΔC set1Δ clr4Δ are lethal (S1D Fig). ------- COMMENT: 3b1166bb2423f4aa 77 M6GZkpv0Od0w1qU37JYq1qOOmMY To our surprise, clr4Δ increases the enrichments of Lsd1/2 while set1Δ decreases the enrichments of Lsd1 at its enriched genomic loci (Figs 2G, 2H, S6 and S7). ------- COMMENT: 3b1166bb2423f4aa 78 M6GZkpv0Od0w1qU37JYq1qOOmMY To our surprise, clr4Δ increases the enrichments of Lsd1/2 while set1Δ decreases the enrichments of Lsd1 at its enriched genomic loci (Figs 2G, 2H, S6 and S7). ------- COMMENT: 3b1166bb2423f4aa 79 M6GZkpv0Od0w1qU37JYq1qOOmMY To our surprise, clr4Δ increases the enrichments of Lsd1/2 while set1Δ decreases the enrichments of Lsd1 at its enriched genomic loci (Figs 2G, 2H, S6 and S7). ------- COMMENT: 3b1166bb2423f4aa 89 I32rDfY84/f8UVHmnccbX95oBMs However, the loss of Shg1 and Sdc1 seems to have little or no effect on Lsd1 protein levels. ------- COMMENT: 3b1166bb2423f4aa 90 I32rDfY84/f8UVHmnccbX95oBMs However, the loss of Shg1 and Sdc1 seems to have little or no effect on Lsd1 protein levels. ------- COMMENT: 3b1166bb2423f4aa 92 wgeCXuGaf5cC2akDrKe5JQq/nuk Both Lsd1 (Fig 4C) and Lsd2 (Fig 4D) protein levels drastically increase after heat treatment, although the mRNA levels of Lsd1 and Lsd2 were not significantly altered between wild-type and set1Δ cells (S10 Fig), suggesting that enhanced Lsd1/2 proteins are required for cell survival under heat stress [61]. ------- COMMENT: 3b1166bb2423f4aa 93 wgeCXuGaf5cC2akDrKe5JQq/nuk Both Lsd1 (Fig 4C) and Lsd2 (Fig 4D) protein levels drastically increase after heat treatment, although the mRNA levels of Lsd1 and Lsd2 were not significantly altered between wild-type and set1Δ cells (S10 Fig), suggesting that enhanced Lsd1/2 proteins are required for cell survival under heat stress [61]. ------- COMMENT: 3b1166bb2423f4aa 94 tTbvTQNN+OtARbPXmn/AtslEebM Protein levels of Lsd1 and Lsd2 are similar between wild-type and clr4Δ cells at 37 ̊C, indicating that Clr4 is not responsible for Lsd1/2 upregulation in this condition (Fig 4C and 4D) ------- COMMENT: 3b1166bb2423f4aa 95 tTbvTQNN+OtARbPXmn/AtslEebM Protein levels of Lsd1 and Lsd2 are similar between wild-type and clr4Δ cells at 37 ̊C, indicating that Clr4 is not responsible for Lsd1/2 upregulation in this condition (Fig 4C and 4D) ------- COMMENT: 3b1166bb2423f4aa 96 m8deNRV4+WPMau6AWtRrWFHBWYs However, without Set1, Lsd1/2 protein levels are no longer upregulated during heat stress (Fig 4C and 4D), which implies that Set1 is required to elevate the protein levels of Lsd1 and Lsd2 under heat stress. ------- COMMENT: 3b1166bb2423f4aa 97 Wmjm4n+QiraUrcfsT9kgpIPF8S0 In wild-type cells, we observed a significantly increased amount of Set1 at 37 ̊C (Fig 6A), indicating that Set1 is also stabilized during heat stress. ------- COMMENT: 3b1166bb2423f4aa 98 dAr+76hABvK/4YzSqzBWbS2xC3g Under heat stress (37 ̊C), it was consistently found that ubiquitination of Lsd1 and Lsd2 is drastically enhanced in set1Δ cells, even without mts2-1 as a background (Fig 4F) ------- COMMENT: 3b1166bb2423f4aa 99 dAr+76hABvK/4YzSqzBWbS2xC3g Under heat stress (37 ̊C), it was consistently found that ubiquitination of Lsd1 and Lsd2 is drastically enhanced in set1Δ cells, even without mts2-1 as a background (Fig 4F) ------- COMMENT: 3b1166bb2423f4aa 100 rmBBEGo56kfth7eosI1cJFxFaME Intriguingly, about 72% of downregulated genes in lsd1-ΔHMG, lsd2-ΔC, and set1Δ are antisense non-coding RNAs (S1 Table), ------- COMMENT: 3b1166bb2423f4aa 101 rmBBEGo56kfth7eosI1cJFxFaME Intriguingly, about 72% of downregulated genes in lsd1-ΔHMG, lsd2-ΔC, and set1Δ are antisense non-coding RNAs (S1 Table), ------- COMMENT: 3b1166bb2423f4aa 102 rmBBEGo56kfth7eosI1cJFxFaME Intriguingly, about 72% of downregulated genes in lsd1-ΔHMG, lsd2-ΔC, and set1Δ are antisense non-coding RNAs (S1 Table), ------- COMMENT: 3b1166bb2423f4aa 103 rmBBEGo56kfth7eosI1cJFxFaME Intriguingly, about 72% of downregulated genes in lsd1-ΔHMG, lsd2-ΔC, and set1Δ are antisense non-coding RNAs (S1 Table), ------- COMMENT: 3b1166bb2423f4aa 104 tTbvTQNN+OtARbPXmn/AtslEebM Protein levels of Lsd1 and Lsd2 are similar between wild-type and clr4Δ cells at 37 ̊C, indicating that Clr4 is not responsible for Lsd1/2 upregulation in this condition (Fig 4C and 4D) ------- COMMENT: 3b1166bb2423f4aa 105 f9YU7sLyyh3q7zv8CBgIOYGArjI Set1 signif- icantly at 37 ̊C compared to that at 30 ̊C, which is due to the temperature sensitive nature of cul4-1. ------- COMMENT: 3b1166bb2423f4aa 106 f2u1jVHykql6mfiJqJuKccUYcpk At 37 ̊C, only marginal changes in Lsd1 protein levels were observed in H3K4R and H2B-K119R mutants (Fig 6D). However, a significant reduction of Lsd2 was seen in both H3K4R and H2B K119R mutants (Fig 6E), ------- COMMENT: 3b1166bb2423f4aa 107 /+WS65tc/DD30n7xs9eLKKrO5oQ Fig. S1E ------- COMMENT: 3b1166bb2423f4aa 108 /+WS65tc/DD30n7xs9eLKKrO5oQ Fig. S1E ------- COMMENT: 3b3bb128e7747bc0 1 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 3b3bb128e7747bc0 2 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 3b3bb128e7747bc0 3 p23VaE2MmzhdxFHAPnFMA17hdOk Fig. 2A and B ------- COMMENT: 3b3bb128e7747bc0 4 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 3b3bb128e7747bc0 5 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 3b3bb128e7747bc0 6 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 3b3bb128e7747bc0 7 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 3b3bb128e7747bc0 8 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 3b3bb128e7747bc0 9 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 3b3bb128e7747bc0 10 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 3b3bb128e7747bc0 11 fW30aVhWks941NA/lHoMBVjgieY Fig. 1D and E ------- COMMENT: 3b3bb128e7747bc0 12 PtWz3QLtFNOk+hQdKIzxMwXnm5Q Fig. 3A and B ------- COMMENT: 3b3bb128e7747bc0 13 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 3b3bb128e7747bc0 14 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 3b3bb128e7747bc0 15 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 3b3bb128e7747bc0 16 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 3b3bb128e7747bc0 17 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 3b3bb128e7747bc0 18 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 3b3bb128e7747bc0 19 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 3b3bb128e7747bc0 20 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 3b3bb128e7747bc0 21 z4AG8tve7cq/N2YwGMKPOcYBdn4 Fig. 3F and G ------- COMMENT: 3b3bb128e7747bc0 22 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 3b3bb128e7747bc0 23 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 3b3bb128e7747bc0 24 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 3b3bb128e7747bc0 25 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 3b3bb128e7747bc0 26 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 3b3bb128e7747bc0 27 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 3b3bb128e7747bc0 28 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 3b3bb128e7747bc0 29 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 3b3bb128e7747bc0 30 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 3b3bb128e7747bc0 31 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 3b3bb128e7747bc0 32 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 3b3bb128e7747bc0 33 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 3b3bb128e7747bc0 34 kWB79+Rma0v6OEhVWsZJGaUvu9U Fig. 4G and H ------- COMMENT: 3b3bb128e7747bc0 35 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 3b3bb128e7747bc0 36 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 3b3bb128e7747bc0 37 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 3b3bb128e7747bc0 38 qvLfj6uBlfZpMczhMXEF9D3liv8 Fig. 7A and B ------- COMMENT: 3b3bb128e7747bc0 39 lt2pkx5XPTUurrYIIsqW8bTRKF8 Fig. 7C ------- COMMENT: 3b3bb128e7747bc0 40 wr5TXzzUQ5a8pjtOfW1cRWvVFaY Fig. 7D ------- COMMENT: 3b3bb128e7747bc0 42 6KeOsSsnVIZZmGmuPdaS/qGNfjI Fig. 7F ------- COMMENT: 3b3bb128e7747bc0 43 CVkVKvsXHG8UAbnXos1ukcCDxw0 Fig. 7G ------- COMMENT: 3b3bb128e7747bc0 45 CVkVKvsXHG8UAbnXos1ukcCDxw0 Fig. 7G ------- COMMENT: 3b3bb128e7747bc0 46 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 3b3bb128e7747bc0 47 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 3b3bb128e7747bc0 48 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 3b3bb128e7747bc0 50 lz9TdyXdluWGn3h6oHlOykmxoXA in the present study we show that the DNA-binding activity of Cbf11 is implicated in all its known functions, with the regulation of lipid metabolism genes remaining the best characterized role of Cbf11. ------- COMMENT: 3b3bb128e7747bc0 57 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 3b3bb128e7747bc0 58 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 3b3bb128e7747bc0 59 fW30aVhWks941NA/lHoMBVjgieY Fig. 1D and E ------- COMMENT: 3b3bb128e7747bc0 60 fW30aVhWks941NA/lHoMBVjgieY Fig. 1D and E ------- COMMENT: 3b3bb128e7747bc0 61 FnoXrkDUavUxfTCif/1riD739+k Fig. S2C ------- COMMENT: 3b3bb128e7747bc0 62 FnoXrkDUavUxfTCif/1riD739+k Fig. S2C ------- COMMENT: 3b3bb128e7747bc0 63 FnoXrkDUavUxfTCif/1riD739+k Fig. S2C ------- COMMENT: 3b3bb128e7747bc0 64 FnoXrkDUavUxfTCif/1riD739+k Fig. S2C ------- COMMENT: 3b3bb128e7747bc0 65 FnoXrkDUavUxfTCif/1riD739+k Fig. S2C ------- COMMENT: 3b3bb128e7747bc0 66 FnoXrkDUavUxfTCif/1riD739+k Fig. S2C ------- COMMENT: 3b3bb128e7747bc0 67 FnoXrkDUavUxfTCif/1riD739+k Fig. S2C ------- COMMENT: 3b3bb128e7747bc0 68 FnoXrkDUavUxfTCif/1riD739+k Fig. S2C ------- COMMENT: 3b3bb128e7747bc0 69 p23VaE2MmzhdxFHAPnFMA17hdOk Fig. 2A and B ------- COMMENT: 3b3bb128e7747bc0 70 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 3b3bb128e7747bc0 71 PtWz3QLtFNOk+hQdKIzxMwXnm5Q Fig. 3A and B ------- COMMENT: 3b3bb128e7747bc0 72 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 3b3bb128e7747bc0 73 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 3b3bb128e7747bc0 74 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 3b3bb128e7747bc0 75 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 3b3bb128e7747bc0 76 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 3b3bb128e7747bc0 77 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 3b3bb128e7747bc0 78 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 3b3bb128e7747bc0 79 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 3b3bb128e7747bc0 80 z4AG8tve7cq/N2YwGMKPOcYBdn4 Fig. 3F and G ------- COMMENT: 3b3bb128e7747bc0 81 z4AG8tve7cq/N2YwGMKPOcYBdn4 Fig. 3F and G ------- COMMENT: 3b3bb128e7747bc0 82 EJrKWyBr/NaQhxxvZLUQXKV3oNU Fig. S2E ------- COMMENT: 3b3bb128e7747bc0 83 EJrKWyBr/NaQhxxvZLUQXKV3oNU Fig. S2E ------- COMMENT: 3b3bb128e7747bc0 84 EJrKWyBr/NaQhxxvZLUQXKV3oNU Fig. S2E ------- COMMENT: 3b3bb128e7747bc0 85 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 3b3bb128e7747bc0 86 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 3bc471528952a5da 1 1v0LcLDBlzT/RnXKFGjn9FmLG/I Tra1-dependent ------- COMMENT: 3bc471528952a5da 9 1v0LcLDBlzT/RnXKFGjn9FmLG/I (comment: Tra1-dependent) ------- COMMENT: 3bc471528952a5da 30 +XG4Q9lfPC24RZR5ZThsd1TXCUI (comment: Rep2 locates SAGA complex at MBF-regulated promoters.) ------- COMMENT: 3bc471528952a5da 34 /q3Lb1pTZDuMZyJM2R+89uetQ+c (comment: chromatin association at MCBs is part of positive regulation of G1/S transition of mitotic cell cycle) ------- COMMENT: 3bdb6bca18decfc1 4 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 3bdb6bca18decfc1 5 4poaw3Wm9l5VLOEkTWObQNYCeVA fig 2f ------- COMMENT: 3bfc9f7d307c2e12 1 pssxQkDRvQYvRtq+UPaWL9ESQkM Figure 1B, 1D-F, 2B-E, 3A-B, S1A-B ------- COMMENT: 3bfc9f7d307c2e12 2 F5SP6egrnIP09MTZM/f98fLTaZ4 (comment: CHECK in vitro binding assay) Figure 3B ------- COMMENT: 3bfc9f7d307c2e12 3 j+27s870bT4eRiUk4eyudO7m8zU (comment: in vitro binding assay with purified Cdc15 F-BAR domain and purified Pxl1) (Fig 1E) ------- COMMENT: 3bfc9f7d307c2e12 5 /fjGd23wa+KbW6ScmyQndBQSZss Figure 4A,C ------- COMMENT: 3bfc9f7d307c2e12 6 /fjGd23wa+KbW6ScmyQndBQSZss Figure 4A,C ------- COMMENT: 3bfc9f7d307c2e12 7 MdH7t4v9t6WLoQTYFXbsSwt5f6A Figure 4B,D ------- COMMENT: 3bfc9f7d307c2e12 8 MdH7t4v9t6WLoQTYFXbsSwt5f6A Figure 4B,D ------- COMMENT: 3bfc9f7d307c2e12 9 MdH7t4v9t6WLoQTYFXbsSwt5f6A Figure 4B,D ------- COMMENT: 3bfc9f7d307c2e12 10 0Lz7+EKRtk80kgPbujpveuPmU9Y Figure S1D-E ------- COMMENT: 3bfc9f7d307c2e12 11 apDuTYstO05toxLsldP+/TXtckc Figure S2D (comment: CONDITION 19 degrees C) ------- COMMENT: 3bfc9f7d307c2e12 12 iP0+3o9S8aIJhn+HFTqxCbMs4DM Figure S2D ------- COMMENT: 3bfc9f7d307c2e12 13 nHSsN4p5y0q4dCb21h6c7HxCLPY Figure S2D(25,29,32) ------- COMMENT: 3bfc9f7d307c2e12 14 iP0+3o9S8aIJhn+HFTqxCbMs4DM Figure S2D ------- COMMENT: 3bfc9f7d307c2e12 15 a604+jvFKhrWMcbUFK6d2+LN8xI Fig S2D ------- COMMENT: 3bfc9f7d307c2e12 16 iP0+3o9S8aIJhn+HFTqxCbMs4DM Figure S2D ------- COMMENT: 3bfc9f7d307c2e12 17 iP0+3o9S8aIJhn+HFTqxCbMs4DM Figure S2D ------- COMMENT: 3bfc9f7d307c2e12 18 iP0+3o9S8aIJhn+HFTqxCbMs4DM Figure S2D ------- COMMENT: 3bfc9f7d307c2e12 19 iP0+3o9S8aIJhn+HFTqxCbMs4DM Figure S2D ------- COMMENT: 3bfc9f7d307c2e12 20 4W9byHm3HPPH/O50uJB1jMP+HTs Figure S2D (25,29,32) ------- COMMENT: 3bfc9f7d307c2e12 21 4W9byHm3HPPH/O50uJB1jMP+HTs Figure S2D (25,29,32) ------- COMMENT: 3bfc9f7d307c2e12 22 iP0+3o9S8aIJhn+HFTqxCbMs4DM Figure S2D ------- COMMENT: 3bfc9f7d307c2e12 23 iP0+3o9S8aIJhn+HFTqxCbMs4DM Figure S2D ------- COMMENT: 3bfc9f7d307c2e12 24 a604+jvFKhrWMcbUFK6d2+LN8xI Fig S2D ------- COMMENT: 3bfc9f7d307c2e12 25 a604+jvFKhrWMcbUFK6d2+LN8xI Fig S2D ------- COMMENT: 3bfc9f7d307c2e12 26 aZ1Nkw7JS0Ts3WneOWmtPaPLhpU Figure S2A-C ------- COMMENT: 3bfc9f7d307c2e12 27 aZ1Nkw7JS0Ts3WneOWmtPaPLhpU Figure S2A-C ------- COMMENT: 3bfc9f7d307c2e12 28 aZ1Nkw7JS0Ts3WneOWmtPaPLhpU Figure S2A-C ------- COMMENT: 3bfc9f7d307c2e12 29 aZ1Nkw7JS0Ts3WneOWmtPaPLhpU Figure S2A-C ------- COMMENT: 3bfc9f7d307c2e12 30 aZ1Nkw7JS0Ts3WneOWmtPaPLhpU Figure S2A-C ------- COMMENT: 3bfc9f7d307c2e12 31 aZ1Nkw7JS0Ts3WneOWmtPaPLhpU Figure S2A-C ------- COMMENT: 3bfc9f7d307c2e12 32 l4gKQzXSsXilpF046kSxIB837no (comment: Pxl1 full-length + Cdc15 F-BAR) (Figure 1B), (comment: Pxl1 aa3-27 + Cdc15 F-BAR) (Figure 1D), (comment: Cdc15(aa600-end) + Pxl1 full-length) (Figure 2C), (comment: Cdc15(aa600-end) + Pxl1 aa177-188) (Figure 2D), (comment: Cdc15-SH3 + Pxl1 aa177-188) (Figure 2D), (comment: Cdc15 full-length + Pxl1 full-length) (Figure 3A-B) ------- COMMENT: 3bfc9f7d307c2e12 33 fiqG2BZxs7VJa7hLe+Sn3UoBqms Figure 2B Pxl1(AxxA1-6) reduced binding to Cdc15C(aa441-end) compared to wildtype Pxl1 ------- COMMENT: 3bfc9f7d307c2e12 34 34nj4071ikSBvU+Y2o+J1rtqPKc Pxl1 (aa177-188 P181A, P184A) abolished binding to Cdc15 SH3 and Cdc15C1(aa600-end), Figure 2D ------- COMMENT: 3bfc9f7d307c2e12 35 b9oCapi/21zXvq5N3bOa7YalbWk Pxl1-AxxA6 reduced binding to full-length Cdc15 (Figure 3A) and Cdc15 C1 (Figure S1A) ------- COMMENT: 3bfc9f7d307c2e12 36 g46Q2iTasVmo5SlO69nu6cTzog4 Pxl1-P18A+AxxA6 reduced binding to full-length Cdc15 compared to wild type Pxl1 (Figure 3A) ------- COMMENT: 3bfc9f7d307c2e12 37 Uvl4Fjy/y7fygPuvWHW7DOJ/H54 Pxl1-P18A reduced binding to full-length Cdc15 compared to wild-type Pxl1 (Figure 3A) ------- COMMENT: 3bfc9f7d307c2e12 38 s2Pr3hhBUWaqiZlv7rHROC81Txo Pxl1-AxxA1-3 bound Cdc15C1(aa600-end) just as well as wild type Pxl1 (Figure S1A) ------- COMMENT: 3bfc9f7d307c2e12 39 2SxfXc0S4VRy4Dd/VuTVeF2ktBQ Mutant reduced binding to Cdc15C1(aa600-end) compared to wild type Pxl1 (Figure S1A) ------- COMMENT: 3bfc9f7d307c2e12 40 2SxfXc0S4VRy4Dd/VuTVeF2ktBQ Mutant reduced binding to Cdc15C1(aa600-end) compared to wild type Pxl1 (Figure S1A) ------- COMMENT: 3bfc9f7d307c2e12 41 2SxfXc0S4VRy4Dd/VuTVeF2ktBQ Mutant reduced binding to Cdc15C1(aa600-end) compared to wild type Pxl1 (Figure S1A) ------- COMMENT: 3bfc9f7d307c2e12 42 AtWQ5RIAjreTEt5DkBFKKV89p3o Mutant bound Cdc15C1(aa600-end) as well as wildtype Pxl1 (Figure S1A) ------- COMMENT: 3bfc9f7d307c2e12 43 o8pA2du8jQbTe2TSQsL3eTs3s8M Figure S3A (comment: CONDITION 25C) ------- COMMENT: 3bfc9f7d307c2e12 44 moiPI6AaR6QMWAnNgd+dkw6vDag Figure S3A (comment: CONDITION 25C) ------- COMMENT: 3bfc9f7d307c2e12 45 moiPI6AaR6QMWAnNgd+dkw6vDag Figure S3A (comment: CONDITION 25C) ------- COMMENT: 3bfc9f7d307c2e12 46 o8pA2du8jQbTe2TSQsL3eTs3s8M Figure S3A (comment: CONDITION 25C) ------- COMMENT: 3bfc9f7d307c2e12 47 VNE9DTUQq+hIuZaUr+3fdGN2BOM Figure S3A ------- COMMENT: 3bfc9f7d307c2e12 48 moiPI6AaR6QMWAnNgd+dkw6vDag Figure S3A (comment: CONDITION 25C) ------- COMMENT: 3bfc9f7d307c2e12 49 moiPI6AaR6QMWAnNgd+dkw6vDag Figure S3A (comment: CONDITION 25C) ------- COMMENT: 3bfc9f7d307c2e12 50 o8pA2du8jQbTe2TSQsL3eTs3s8M Figure S3A (comment: CONDITION 25C) ------- COMMENT: 3bfc9f7d307c2e12 51 moiPI6AaR6QMWAnNgd+dkw6vDag Figure S3A (comment: CONDITION 25C) ------- COMMENT: 3bfc9f7d307c2e12 52 14pXtrpgGT/ZvgPBu3nvOOluGsU Figure S3B (comment: CONDITION 25C) ------- COMMENT: 3bfc9f7d307c2e12 53 rzoapsxxo5AQc2NAakt5rfXh8WQ Figure S2E ------- COMMENT: 3bfc9f7d307c2e12 54 rzoapsxxo5AQc2NAakt5rfXh8WQ Figure S2E ------- COMMENT: 3bfc9f7d307c2e12 55 rzoapsxxo5AQc2NAakt5rfXh8WQ Figure S2E ------- COMMENT: 3bfc9f7d307c2e12 56 rzoapsxxo5AQc2NAakt5rfXh8WQ Figure S2E ------- COMMENT: 3bfc9f7d307c2e12 57 rzoapsxxo5AQc2NAakt5rfXh8WQ Figure S2E ------- COMMENT: 3bfc9f7d307c2e12 58 rzoapsxxo5AQc2NAakt5rfXh8WQ Figure S2E ------- COMMENT: 3bfc9f7d307c2e12 59 rzoapsxxo5AQc2NAakt5rfXh8WQ Figure S2E ------- COMMENT: 3bfc9f7d307c2e12 60 rzoapsxxo5AQc2NAakt5rfXh8WQ Figure S2E ------- COMMENT: 3bfc9f7d307c2e12 61 rzoapsxxo5AQc2NAakt5rfXh8WQ Figure S2E ------- COMMENT: 3bfc9f7d307c2e12 62 rzoapsxxo5AQc2NAakt5rfXh8WQ Figure S2E ------- COMMENT: 3bfc9f7d307c2e12 63 rzoapsxxo5AQc2NAakt5rfXh8WQ Figure S2E ------- COMMENT: 3bfc9f7d307c2e12 64 rzoapsxxo5AQc2NAakt5rfXh8WQ Figure S2E ------- COMMENT: 3bfc9f7d307c2e12 65 rzoapsxxo5AQc2NAakt5rfXh8WQ Figure S2E ------- COMMENT: 3bfc9f7d307c2e12 66 rzoapsxxo5AQc2NAakt5rfXh8WQ Figure S2E ------- COMMENT: 3bfc9f7d307c2e12 67 rzoapsxxo5AQc2NAakt5rfXh8WQ Figure S2E ------- COMMENT: 3bfc9f7d307c2e12 68 rzoapsxxo5AQc2NAakt5rfXh8WQ Figure S2E ------- COMMENT: 3bfc9f7d307c2e12 69 rzoapsxxo5AQc2NAakt5rfXh8WQ Figure S2E ------- COMMENT: 3bfc9f7d307c2e12 70 rzoapsxxo5AQc2NAakt5rfXh8WQ Figure S2E ------- COMMENT: 3c257a83101c8398 1 yY4Ru0Dp7IA/rjeWUyVOtxSz060 Therefore, Chp1 protein was also involved in the production or processing of centromeric RNA transcripts, which might be linked to heterochromatin estab- lishment. ------- COMMENT: 3c257a83101c8398 2 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3c257a83101c8398 3 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3c257a83101c8398 4 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3c257a83101c8398 5 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3c257a83101c8398 6 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3c257a83101c8398 7 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3c257a83101c8398 8 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3c257a83101c8398 9 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3c257a83101c8398 10 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3c257a83101c8398 11 I2Klv+cF3/W5PPJssdOU+JwBtmw We found that the association of Chp1-13myc with the three heterochromatic regions (CEN, MAT, and TEL) was not affected in the absence of swi6 þ or chp2 þ (Figure 2A). ------- COMMENT: 3c257a83101c8398 12 I2Klv+cF3/W5PPJssdOU+JwBtmw We found that the association of Chp1-13myc with the three heterochromatic regions (CEN, MAT, and TEL) was not affected in the absence of swi6 þ or chp2 þ (Figure 2A). ------- COMMENT: 3c257a83101c8398 13 I2Klv+cF3/W5PPJssdOU+JwBtmw We found that the association of Chp1-13myc with the three heterochromatic regions (CEN, MAT, and TEL) was not affected in the absence of swi6 þ or chp2 þ (Figure 2A). ------- COMMENT: 3c257a83101c8398 14 I2Klv+cF3/W5PPJssdOU+JwBtmw We found that the association of Chp1-13myc with the three heterochromatic regions (CEN, MAT, and TEL) was not affected in the absence of swi6 þ or chp2 þ (Figure 2A). ------- COMMENT: 3c257a83101c8398 15 I2Klv+cF3/W5PPJssdOU+JwBtmw We found that the association of Chp1-13myc with the three heterochromatic regions (CEN, MAT, and TEL) was not affected in the absence of swi6 þ or chp2 þ (Figure 2A). ------- COMMENT: 3c257a83101c8398 16 I2Klv+cF3/W5PPJssdOU+JwBtmw We found that the association of Chp1-13myc with the three heterochromatic regions (CEN, MAT, and TEL) was not affected in the absence of swi6 þ or chp2 þ (Figure 2A). ------- COMMENT: 3c257a83101c8398 17 iTpbK2hEsCcy6czc3bz5KdMpxyI In contrast, the centromeric localization of Swi6 or Chp2-13myc was specifically de- creased in the Dchp1 cells (Figures 2B and C, Dchp1) ------- COMMENT: 3c257a83101c8398 18 iTpbK2hEsCcy6czc3bz5KdMpxyI In contrast, the centromeric localization of Swi6 or Chp2-13myc was specifically de- creased in the Dchp1 cells (Figures 2B and C, Dchp1) ------- COMMENT: 3c257a83101c8398 19 bM3ZsZoHrGn9LOZFcW+iQ3XJkcQ Interestingly, Swi6 was required for the localization of Chp2 to the mating-type region or telomeres but not to the centromeres (Figure 2B, Dswi6). ------- COMMENT: 3c257a83101c8398 20 bM3ZsZoHrGn9LOZFcW+iQ3XJkcQ Interestingly, Swi6 was required for the localization of Chp2 to the mating-type region or telomeres but not to the centromeres (Figure 2B, Dswi6). ------- COMMENT: 3c257a83101c8398 21 bM3ZsZoHrGn9LOZFcW+iQ3XJkcQ Interestingly, Swi6 was required for the localization of Chp2 to the mating-type region or telomeres but not to the centromeres (Figure 2B, Dswi6). ------- COMMENT: 3c257a83101c8398 22 l3ai4330ma8wv/meVtk5HDXhjHQ Figure 1 ------- COMMENT: 3c257a83101c8398 23 l3ai4330ma8wv/meVtk5HDXhjHQ Figure 1 ------- COMMENT: 3c257a83101c8398 24 l3ai4330ma8wv/meVtk5HDXhjHQ Figure 1 ------- COMMENT: 3c257a83101c8398 25 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3c257a83101c8398 26 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3c257a83101c8398 27 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3c257a83101c8398 28 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3c257a83101c8398 29 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3c257a83101c8398 30 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3c257a83101c8398 31 SEY5KQwcSt4tT35TN/kg639BIKY The present data also demonstrate that Chp1 function is required not only for the centromeres but also for the mating-type region and telomeres. ------- COMMENT: 3c257a83101c8398 32 SEY5KQwcSt4tT35TN/kg639BIKY The present data also demonstrate that Chp1 function is required not only for the centromeres but also for the mating-type region and telomeres. ------- COMMENT: 3c257a83101c8398 33 0RuKyerlcFcF439RukiioRRQkKY Intriguingly, we found that, even in the Dchp1 cells, histone H3 in native centromeric heterochromatin (CEN-dg223 locus) remained methylated at lysine 9 (Figure 4B, Dchp1). ------- COMMENT: 3c257a83101c8398 34 FkBWpVyfvJtJchNaacNbx1/oUbg n Dswi6 or Dchp2 cells, the three heterochromatic regions were enriched in K9-methylated H3 at the same level as in wild-type cells (Figure 5B). ------- COMMENT: 3c257a83101c8398 35 0RuKyerlcFcF439RukiioRRQkKY Intriguingly, we found that, even in the Dchp1 cells, histone H3 in native centromeric heterochromatin (CEN-dg223 locus) remained methylated at lysine 9 (Figure 4B, Dchp1). ------- COMMENT: 3c257a83101c8398 36 qvBHA20ue2sPZbiFmGJrfPbXj38 Interestingly, we found that swi6þ dele- tion caused a loss of the H3-K9 methylation at CEN (dg223) in the Dchp1 background (Figure 5D, Dchp1Dswi6), suggesting that Swi6 is required for the maintenance of centromeric H3- K9 methylation in the Dchp1 strain. ------- COMMENT: 3c257a83101c8398 37 IR3YwRiRPT/6Ii6p0AnJ4rcTgcY nexpectedly, the cen- tromeric H3-K9 methylation was also severely decreased in the Dchp1Dchp2 strain (Figure 5D, Dchp1Dchp2). ------- COMMENT: 3c257a83101c8398 38 Jk5/ILM1eOZZPODKmWDz99dCnqk Interestingly, we found that, although deletion of either swi6 þ or chp2 þ did not affect the H3-K9 methylation at MAT (K-R) and TEL (E12) in the Dchp1 background, the methylation level in these regions was severely decreased in the triple-mutant strain (Figure 5D, Dchp1Dchp2Dswi6). Again, these results demonstrate that Swi6 and Chp2 are required for the maintenance of H3-K9 methylation at the three heterochromatic regions, and also indicate that Swi6 and Chp2 have overlapping functions in the maintenance of H3-K9 methylation. ------- COMMENT: 3c257a83101c8398 39 zZpNmppTRRc53zcxic38aZdYYwU We found that H3-K9 methylation at the three heterochromatic regions (CEN- dg223, MAT-K-R, or TEL-E12) was reduced to a level compar- able to that in Dclr4 (Figure 6A), ------- COMMENT: 3c257a83101c8398 40 zZpNmppTRRc53zcxic38aZdYYwU We found that H3-K9 methylation at the three heterochromatic regions (CEN- dg223, MAT-K-R, or TEL-E12) was reduced to a level compar- able to that in Dclr4 (Figure 6A), ------- COMMENT: 3c257a83101c8398 41 zZpNmppTRRc53zcxic38aZdYYwU We found that H3-K9 methylation at the three heterochromatic regions (CEN- dg223, MAT-K-R, or TEL-E12) was reduced to a level compar- able to that in Dclr4 (Figure 6A), ------- COMMENT: 3c257a83101c8398 42 zZpNmppTRRc53zcxic38aZdYYwU We found that H3-K9 methylation at the three heterochromatic regions (CEN- dg223, MAT-K-R, or TEL-E12) was reduced to a level compar- able to that in Dclr4 (Figure 6A), ------- COMMENT: 3c257a83101c8398 43 zZpNmppTRRc53zcxic38aZdYYwU We found that H3-K9 methylation at the three heterochromatic regions (CEN- dg223, MAT-K-R, or TEL-E12) was reduced to a level compar- able to that in Dclr4 (Figure 6A), ------- COMMENT: 3c257a83101c8398 44 zZpNmppTRRc53zcxic38aZdYYwU We found that H3-K9 methylation at the three heterochromatic regions (CEN- dg223, MAT-K-R, or TEL-E12) was reduced to a level compar- able to that in Dclr4 (Figure 6A), ------- COMMENT: 3c257a83101c8398 45 8Xx0Owz86AyYC8zHKs1YpK+hFuk Rik1-13myc was efficiently co-precipitated with 5FLAG-Clr4, and the association was confirmed in the reci- procal IP experiments (Figure 6D). ------- COMMENT: 3c257a83101c8398 46 8Xx0Owz86AyYC8zHKs1YpK+hFuk Rik1-13myc was efficiently co-precipitated with 5FLAG-Clr4, and the association was confirmed in the reci- procal IP experiments (Figure 6D). ------- COMMENT: 3c257a83101c8398 47 40yx3C4u4TGCM6l/QjR9kRzETwA H3-K9 methylation at the three heterochromatic regions (CEN-dg223,MAT-K-R, or TEL-E12) was reduced to a level comparable to that in Dclr4 (Figure 6A), suggesting that Rik1 has a critical role in H3-K9 methylation at the native heterochromatic regions. ------- COMMENT: 3c257a83101c8398 48 40yx3C4u4TGCM6l/QjR9kRzETwA H3-K9 methylation at the three heterochromatic regions (CEN-dg223,MAT-K-R, or TEL-E12) was reduced to a level comparable to that in Dclr4 (Figure 6A), suggesting that Rik1 has a critical role in H3-K9 methylation at the native heterochromatic regions. ------- COMMENT: 3c257a83101c8398 49 40yx3C4u4TGCM6l/QjR9kRzETwA H3-K9 methylation at the three heterochromatic regions (CEN-dg223,MAT-K-R, or TEL-E12) was reduced to a level comparable to that in Dclr4 (Figure 6A), suggesting that Rik1 has a critical role in H3-K9 methylation at the native heterochromatic regions. ------- COMMENT: 3c2cf7f390975961 56 4/CfRRKaR1Q2KdFLrMoRkxpmhHo ------- COMMENT: 3c2cf7f390975961 109 dRwpjJh0eZrPl1vQIM4kNoZ86ps This is inferred from a combination of genetic interactions, localizations and phenocopy experiments, it has not been directly assayed but it feels 'safe' (VW) ------- COMMENT: 3c565105aae56085 10 jnKxlBlpu+LlIX15m+pxHf8kDa0 (comment: CHECK inhibited_by zinc(2+) activated_by magnesium(2+)) ------- COMMENT: 3c76dc8ec0d97857 32 rAxGqKue1FnjLSq3l+O1y0COI1g (comment: unphosphorylated form) ------- COMMENT: 3c804802186d667e 1 +V28ll47w+B6Z4amY3umRFvRKGU (comment: affecting highly transcribed genes, antisense transcription of tDNA and rDNA) ------- COMMENT: 3c804802186d667e 3 P9ShdzaS/+Y8Cw0IXYUHbOE5mxE (comment: Affecting Dcr1-terminated genes) ------- COMMENT: 3c804802186d667e 4 wPZUrGyv611kYJrYdE9HEEMWJ1s (comment: affecting antisense transcription at tDNA) ------- COMMENT: 3c804802186d667e 5 4SiuzWQumKrTRhLmZDF5F+y0+J4 (comment: affecting antisense transcription at rDNA) ------- COMMENT: 3c804802186d667e 6 B5HwTkeqUbiCdCpTW3pUU5O3jyc (comment: Affecting Rad52 enrichment at rDNA) ------- COMMENT: 3c804802186d667e 10 ippY9JDQv3ycmCwHFaIRhCOstyQ (comment: occurs at rDNA, tRNA gene, protein coding gene) ------- COMMENT: 3c804802186d667e 11 +V28ll47w+B6Z4amY3umRFvRKGU (comment: affecting highly transcribed genes, antisense transcription of tDNA and rDNA) ------- COMMENT: 3c804802186d667e 12 +V28ll47w+B6Z4amY3umRFvRKGU (comment: affecting highly transcribed genes, antisense transcription of tDNA and rDNA) ------- COMMENT: 3c804802186d667e 14 ZMSFSe8emG0J8YUUNec2gKd1UnA (comment: occurs at tDNA) ------- COMMENT: 3c804802186d667e 15 B5HwTkeqUbiCdCpTW3pUU5O3jyc (comment: Affecting Rad52 enrichment at rDNA) ------- COMMENT: 3c804802186d667e 22 ippY9JDQv3ycmCwHFaIRhCOstyQ (comment: occurs at rDNA, tRNA gene, protein coding gene) ------- COMMENT: 3c804802186d667e 23 ippY9JDQv3ycmCwHFaIRhCOstyQ (comment: occurs at rDNA, tRNA gene, protein coding gene) ------- COMMENT: 3c9ecc4a8f0eb159 1 g9B83ZlHFtL3xNRsxo5953989HA Molecular mechanism of Rad51-driven strand exchange activation by Swi5-Sfr1 ------- COMMENT: 3c9ecc4a8f0eb159 2 g9B83ZlHFtL3xNRsxo5953989HA Molecular mechanism of Rad51-driven strand exchange activation by Swi5-Sfr1 ------- COMMENT: 3cb710700bc0a9b7 3 worF6VXT7ySlZ7OkL4qCdjUgBwY deletion of Sre1 aa 877-900 also destabilized Sre1 and prevented proteolytic activation ------- COMMENT: 3cb710700bc0a9b7 4 GBvJj8c5M/2Xg7hNi2BOMt+Lwvk mutation destabilized Sre1 precursor and prevented Sre1 proteolytic cleavage ------- COMMENT: 3cb710700bc0a9b7 5 GBvJj8c5M/2Xg7hNi2BOMt+Lwvk mutation destabilized Sre1 precursor and prevented Sre1 proteolytic cleavage ------- COMMENT: 3cd4cea969e25e08 2 FAatom/DeB5MGHJqkppttWe5Lwg ------- COMMENT: 3d3eef26565fa966 1 s8zxygB4j9+cKrsniSSlYhY+9P0 Fig5A ------- COMMENT: 3d3eef26565fa966 3 nCrI6Wg8ITKeITi+W8BIOpMXNfY Figure 1, 4,5 ------- COMMENT: 3d3eef26565fa966 4 VFkskau1qxV6L9SVWfIqbfw500g Fig 5A ------- COMMENT: 3d3eef26565fa966 5 VFkskau1qxV6L9SVWfIqbfw500g Fig 5A ------- COMMENT: 3d3eef26565fa966 6 /HDWvtMwvJY3pt9c/Wx7sUk385U Fig 5C cyclin cdc13 cdc2 complex are not detected when cells are blocked in G1 with cdc10-129 mutant complex precipitated with p13 beads ------- COMMENT: 3d3eef26565fa966 7 xAKzpUUQ1Ks6akHbUDkDxomK4SM Fig 5B (comment: CHECK cdc22-M45 blocked in G1/S) ------- COMMENT: 3d3eef26565fa966 8 4q2vZo/Tg+dogq8uTfCz3XW+6XE Fig 5B (comment: CHECK cdc22-M45 blocks in G1/S) ------- COMMENT: 3d3eef26565fa966 9 Y/na1yscLTh0nVWojbjBAhudloQ Fig 7A 8 ------- COMMENT: 3d3eef26565fa966 10 gibMY2kyPUVnttyLd+MZx1R+UUs Fig 6 top panels , Fig7B panel 2 cells examined 7 hour after refeeding with nitrogen After 7 hour the cdc10-V50 cells start to leak through and this allows the mik1D wee1-50 cells to start entering mitosis ------- COMMENT: 3d3eef26565fa966 11 K1PQtVpr07fmsOF6C4geSy1l7bA Fig 6 top panels, Fig7B panel 1 cells examined 7 hour after refeeding with nitrogen ------- COMMENT: 3d3eef26565fa966 12 kIAr9tCRYaJ1E8Rv1sDI7pYOeE4 Fig 6 middle panels Fig7B panel 3 cells examined 7 hour after refeeding with nitrogen ------- COMMENT: 3d3eef26565fa966 13 23wnt7t0NLqvDpi5UNF1UBQt3Zg Fig 6 middle panels Fig7B panel 4 cells examined 7 hour after refeeding with nitrogen ------- COMMENT: 3d3eef26565fa966 15 TZGGADpzSq/pLJm8fHB8HVaLFwA Fig 6 bottom panels Fig7B panel 5 cells nitrogen starved and examined 7 hour after refeeding with nitrogen ------- COMMENT: 3d3eef26565fa966 16 uFAbnPssGKVPA2+DW8XIHrrqUxQ Fig 6 bottom panels Fig7B panel 6 cells examined 7 hour after refeeding with nitrogen ------- COMMENT: 3d3eef26565fa966 21 ekRC727BysHho5LxXT4lI2JhJP8 (comment: CHECK fypo/issues/3164) Fig 5C the cyclin cdc13 cdc2 complex are detected when cells are blocked at G1/S with cdc20-M10 mutant, complex precipitated with p13 beads ------- COMMENT: 3d3eef26565fa966 22 m50sK/mvi804uDPkGQcY9w4790o (comment: CHECK fypo/issues/3164) Fig 5C the cyclin cdc13 cdc2 complex are detected when cells are blocked at G1/S with cdc20-M45 mutant, complex precipitated with p13 beads ------- COMMENT: 3d3eef26565fa966 23 NxrV4kZrpRsH12pdngVDYqIFu6s Fig 6, Fig 7B panel 8 ------- COMMENT: 3d3eef26565fa966 24 SnVrKPZvLvpNBF5+QVqbinZo1Jo Fig 7A panel 2 ------- COMMENT: 3d3eef26565fa966 25 zGy4NrgIUFwwk7Vr5otrsexTN2c Fig 7A panel 1 ------- COMMENT: 3d3eef26565fa966 26 vILWoh5kYv3STfdDPP9LPrRgz+Y Fig 7A panel 4 ------- COMMENT: 3d3eef26565fa966 27 2K82uf7cRHgmsblsPRXpB7DB+ZI Fig 7A panel 6 ------- COMMENT: 3d3eef26565fa966 28 Q7mjsv/Em5tx6O4nj8BaLvyztQ4 Fig 7A panel 3 ------- COMMENT: 3d3eef26565fa966 29 3RtROXkTdJhcQSmJi1h3aSpoOG0 Fig 7A panel 5 ------- COMMENT: 3d3eef26565fa966 30 io3ANfCQHp+OlN1ULRnhilOeMTE Fig 5B (comment: CHECK cdc10-129 cells blocked in G1) ------- COMMENT: 3d3eef26565fa966 31 wlfKELQf/IKYM3k8IjN+k4pjQ/8 Fig 7C ------- COMMENT: 3d3eef26565fa966 32 wlfKELQf/IKYM3k8IjN+k4pjQ/8 Fig 7C ------- COMMENT: 3d3eef26565fa966 33 NKL/ksT95L9Eb7BpWvymPzDROJk Fig 7B. It is the soluble form (upper panel) that disappears not the insoluble form (lower panel) which has implications for which form is allowing replication. I don't know whether to leave this annotation out ------- COMMENT: 3d3eef26565fa966 34 kIvuP91fnGFcasb6gqQ68uC5AyU Fig 7C ------- COMMENT: 3d3eef26565fa966 35 nCrI6Wg8ITKeITi+W8BIOpMXNfY Figure 1, 4,5 ------- COMMENT: 3d43cf9a14d68a43 1 +ggy3PCI+OyGLnyIXUNQwOI/7Bw Figure 5, Figure S4, Movie 5 ------- COMMENT: 3d43cf9a14d68a43 3 +ggy3PCI+OyGLnyIXUNQwOI/7Bw Figure 5, Figure S4, Movie 5 ------- COMMENT: 3d43cf9a14d68a43 4 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3d43cf9a14d68a43 5 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3d43cf9a14d68a43 6 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3d43cf9a14d68a43 7 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3d43cf9a14d68a43 8 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3d43cf9a14d68a43 9 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3d43cf9a14d68a43 10 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3d43cf9a14d68a43 11 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 3d43cf9a14d68a43 13 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 3d43cf9a14d68a43 14 Hp4awc33rVLCTjvUDOeRODw7uPo Figure 3, Figure 4, Figure S2, Movie 2, Movie 3, Movie 4 ------- COMMENT: 3d43cf9a14d68a43 15 mlBWaPsWidGrb7urELtwD6lNPuo Figure 3, Movie 2 ------- COMMENT: 3d43cf9a14d68a43 16 eDubXUzJ9RczOp5ZYQcxRhZjyaE Figure 4, Movie 4 ------- COMMENT: 3d43cf9a14d68a43 17 YRap7elDhYKJbIxidhCkKKKwQ+w Figure 4, Figure S3, Movie 4 ------- COMMENT: 3d43cf9a14d68a43 18 eDubXUzJ9RczOp5ZYQcxRhZjyaE Figure 4, Movie 4 ------- COMMENT: 3d43cf9a14d68a43 19 a9x2o9i4iKck+uFedDHI5Z+QWcU Figure 6, Figure S6, Movie 7 ------- COMMENT: 3d43cf9a14d68a43 20 ZE47xOY76WlhVQ5+oWsRdrUqut0 Figure 7, Movie 9 ------- COMMENT: 3d43cf9a14d68a43 21 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 3d43cf9a14d68a43 22 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 3d43cf9a14d68a43 23 EctPHltWGvGk0lIVrNQwvEA3pho Figure S6, Movie 8 ------- COMMENT: 3d43cf9a14d68a43 24 EctPHltWGvGk0lIVrNQwvEA3pho Figure S6, Movie 8 ------- COMMENT: 3d43cf9a14d68a43 33 FFKGhAKV2BdRhMxVPbiC/2JoEsA Figure 7, Figure S8 ------- COMMENT: 3d43cf9a14d68a43 34 FFKGhAKV2BdRhMxVPbiC/2JoEsA Figure 7, Figure S8 ------- COMMENT: 3d43cf9a14d68a43 35 HBL6UR1SGUpWge6L5BoeOY2F8pA Figure S1 ------- COMMENT: 3d43cf9a14d68a43 36 OCev3eDu2o/WQ2IjRAGX0h+x1fA Figure 2, Movei 1 ------- COMMENT: 3d43cf9a14d68a43 37 JoIMS3TXxOf/rXYRjHYyJvuVzOs Figure 1 (comment: CHECK interphase arrest) ------- COMMENT: 3d43cf9a14d68a43 38 TRLdLLStmlNtw4Rv9YrLufyDSMs Figure 1 (comment: CHECK interphase arrest requested during interphase) ------- COMMENT: 3d43cf9a14d68a43 39 JoIMS3TXxOf/rXYRjHYyJvuVzOs Figure 1 (comment: CHECK interphase arrest) ------- COMMENT: 3d43cf9a14d68a43 40 JoIMS3TXxOf/rXYRjHYyJvuVzOs Figure 1 (comment: CHECK interphase arrest) ------- COMMENT: 3d43cf9a14d68a43 41 D6EkRM6VCgMCUbiu4bOwUa8Wk+0 Figure 2 (comment: CHECK interphase arrest) ------- COMMENT: 3d43cf9a14d68a43 42 9hDcCHMj7MGiWUXNKKpKwjDK648 (comment: PORTLI GROWTH) fig 2 (comment: CHECK interphase arrest https://github.com/pombase/fypo/issues/3339) ------- COMMENT: 3d43cf9a14d68a43 43 +fbc2jd5eOwf9994skILYf5dKqk Figure 3 ------- COMMENT: 3d43cf9a14d68a43 44 +fbc2jd5eOwf9994skILYf5dKqk Figure 3 ------- COMMENT: 3d43cf9a14d68a43 45 HDuGoGva+HBmQKwNTZF9qpE7O6U (comment: PORTLI GROWTH) (Figs 3B and 4B; Movie 2) (comment: CHECK https://github.com/pombase/fypo/issues/3339) ------- COMMENT: 3d43cf9a14d68a43 46 SLwpxk6htS9k8s/JEjUebAP98Rk (comment: PORTLI GROWTH) (Figs 3B and 4B; Movie 2) (comment: CHECK https://github.com/pombase/fypo/issues/3339) ------- COMMENT: 3d43cf9a14d68a43 47 PipavsK3wgIrBLISAlvBmV/8wTM Figure 4,AB ------- COMMENT: 3d43cf9a14d68a43 48 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 3d43cf9a14d68a43 49 eDubXUzJ9RczOp5ZYQcxRhZjyaE Figure 4, Movie 4 ------- COMMENT: 3d43cf9a14d68a43 52 J9cRYjlXdFNSY3fEztLZnKFLXEU Figure 5B ------- COMMENT: 3d43cf9a14d68a43 53 J9cRYjlXdFNSY3fEztLZnKFLXEU Figure 5B ------- COMMENT: 3d43cf9a14d68a43 54 wyae7m0W3fAwMS5v2JW/xxLssUs (comment: PORTLI GROWTH) Figure S6 (comment: CHECK https://github.com/pombase/fypo/issues/3339) ------- COMMENT: 3d43cf9a14d68a43 55 vucmJMgqUOYe15xuQKBEPTsLPHg (comment: PORTLI GROWTH) Figure 6C (comment: CHECK https://github.com/pombase/fypo/issues/3339) ------- COMMENT: 3d43cf9a14d68a43 56 Madt9Y+VqLc2z9IO48f7ER+O1/8 (comment: PORTLI Growth) Figure 6, Figure S6, Movie 7 (comment: CHECK https://github.com/pombase/fypo/issues/3339) ------- COMMENT: 3d43cf9a14d68a43 57 //bwJws5dzugwRB3wmwTJ0Il1zM Figure 7, Movie 9 (comment: CHECK https://github.com/pombase/fypo/issues/3339) ------- COMMENT: 3d43cf9a14d68a43 61 vtMUGDlCnbCDCGcZHEwfoYfptjE Figure 7D ------- COMMENT: 3d43cf9a14d68a43 62 FmsW9x0r2z6mninE9P14wFfce0o Figure 7D ------- COMMENT: 3d43cf9a14d68a43 63 w2rZhBY+lrShUOkRV1+hUc6Gpg8 Figure 7C ------- COMMENT: 3d47d60b7e868097 42 GvzrGMCgnRBdXyr0B/dXWprCBN0 (comment: probably Y173, but not determined experimentally) ------- COMMENT: 3d47d60b7e868097 78 GvzrGMCgnRBdXyr0B/dXWprCBN0 (comment: probably Y173, but not determined experimentally) ------- COMMENT: 3d47d60b7e868097 80 GvzrGMCgnRBdXyr0B/dXWprCBN0 (comment: probably Y173, but not determined experimentally) ------- COMMENT: 3d88b1b5a61259cf 2 555UfNw/MwCj/nF+apt/hgbsMUQ (comment: assayed in strain with cdc10-129 to synchronize) ------- COMMENT: 3dc265797d97f877 3 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig 2 ------- COMMENT: 3dc265797d97f877 5 8rduwvVP8NSowM19jYgt8Nh5Gks fig 6 (comment: it doesn't bind dna according to later studies) ------- COMMENT: 3e04cbe2621b6a70 1 OoiAmCqCJCDdZ+gZm19ZppTtSZg (comment: CHECK mitotic prophase) ------- COMMENT: 3e04cbe2621b6a70 3 WTV5b3CrW5/zMRh7ncaOBAjy0oM (comment: CHECK binds to Mhf2) ------- COMMENT: 3e04cbe2621b6a70 4 sTQmdM8nDDRHnTjfQTE+wI4wDfE (comment: CHECK binds to Mhf1) ------- COMMENT: 3e04cbe2621b6a70 5 z2hNFyi74gRPr2Gly/nCx3zPa8w (comment: CHECK abolished Mhf1 localization) ------- COMMENT: 3e04cbe2621b6a70 9 TCgM0aUbCYV1gv1GXaYGnlpgYhI As shown in Fig. 1A,B, the percentage of mitotic WT cells displaying Plo1–GFP signals increased over time upon incubation at 16°C and, 8 h after cold treatment, ∼90% of WT cells were arrested at preanaphase, presumably due to the activation of the SAC. As Bub1 is a core component of the SAC (Fischer et al., 2021), the absence of Bub1 was expected to abolish the SAC. Consistently, the percentage of mitotic bub1Δ cells remained low (<10%) throughout the period of cold treatment (Fig. 1A,B). ------- COMMENT: 3e04cbe2621b6a70 10 J7qSZy4T3+sXyWPj4IrJWunI1kE Intriguingly, similar to the percentage of mitotic WT cells, the percentage of mitotic mhf2Δ cells increased over time upon cold treatment, but to a lesser degree (Fig. 1A,B). ------- COMMENT: 3e0515d51b776f10 1 SrffArbcFozqexgRsS8sAK3xEb0 replication dynamic analysis demonstrates that the priming strand is stable in the absence of Ku (previous work has shown resection is increased behind the arrested fork). Replication restart is slightly delayed, confirming previous work. Assayed by polymerase usage sequencing ------- COMMENT: 3e0515d51b776f10 2 vpCDnd/fH70duoghOSJCBib1tSk ------- COMMENT: 3e0515d51b776f10 3 EwilNFTJZdBM6oyfnXT7u0owgag Rnh201-RED mutant, based on the S. cerevsiae equivalent, is unable to remove single rNMPs from DNA but, buy genetic analysis, is able to remove runs of rNMPs. ------- COMMENT: 3e0515d51b776f10 5 vpCDnd/fH70duoghOSJCBib1tSk ------- COMMENT: 3e0515d51b776f10 6 vpCDnd/fH70duoghOSJCBib1tSk ------- COMMENT: 3e0a5d273c70b058 17 VXy0vFAxZCBalF4qckqR2pA0LLM Mutations that are predicted to impair middle module sta- bility also lead to a general decrease in RNA synthesis (Extended Data Fig. 4d), showing that the middle module is globally required for transcription. (comment: used txn rather than RNA level because we know it is transcription) ------- COMMENT: 3e0a5d273c70b058 18 VXy0vFAxZCBalF4qckqR2pA0LLM Mutations that are predicted to impair middle module sta- bility also lead to a general decrease in RNA synthesis (Extended Data Fig. 4d), showing that the middle module is globally required for transcription. (comment: used txn rather than RNA level because we know it is transcription) ------- COMMENT: 3e2f643b12155f98 1 dcKKe2u70w/VGhcZxu2FUM1VUoc Figs 1, 2, 3, 4,5 (comment: cells blocked in late G2 and in mid mitosis) ------- COMMENT: 3e2f643b12155f98 2 EiNcXSaJBfvyVrHmU+EVLh2hhUI Fig 6b C-F. (comment: Cells contain cdc2-F15 mutant on multi copy LEU2+ plasmid.) ------- COMMENT: 3e2f643b12155f98 3 LTRzijpLNo8XHhimztFuGW6le60 Fig 6b C-F. (comment: Cells contain cdc2-F15 mutant on multi copy LEU2+ plasmid.) ------- COMMENT: 3e2f643b12155f98 4 LTRzijpLNo8XHhimztFuGW6le60 Fig 6b C-F. (comment: Cells contain cdc2-F15 mutant on multi copy LEU2+ plasmid.) ------- COMMENT: 3e2f643b12155f98 5 LTRzijpLNo8XHhimztFuGW6le60 Fig 6b C-F. (comment: Cells contain cdc2-F15 mutant on multi copy LEU2+ plasmid.) ------- COMMENT: 3e2f643b12155f98 6 4hVU92l/Fs+Gsg1I/fWIKrD2FlY Data not shown, assayed by colony growth on plates ------- COMMENT: 3e2f643b12155f98 7 rz7HiaXi5+YgG2imMaoSz/HxOLM Fig 6B. (comment: Cells contain cdc2-F15 mutant on multi copy LEU2+ plasmid.) ------- COMMENT: 3e2f643b12155f98 8 7pYUXdxOrUFs4xPl3voSQW9tdr4 Fig 6B. (comment: Cells contain cdc2-F19 mutant on multi copy LEU2+ plasmid.) ------- COMMENT: 3e2f643b12155f98 9 iLUw/hkLQ46tLcdUhvaPiTmh+QU Fig 6C. (comment: Cells contain cdc2-F19 mutant on multi copy LEU2+ plasmid.) ------- COMMENT: 3e2f643b12155f98 10 dcKKe2u70w/VGhcZxu2FUM1VUoc Figs 1, 2, 3, 4,5 (comment: cells blocked in late G2 and in mid mitosis) ------- COMMENT: 3e3589df2ec0053d 9 p56tMISueHVkDZeSbjTK/ypSjQM (comment: regulates binding by myosin; assayed in vitro using rabbit actin and unspecified myosin motor domain) ------- COMMENT: 3e4ec1257970269c 18 l+f1R919mLn1hkTFl9hvZaQF59A fig 6 ------- COMMENT: 3e4ec1257970269c 19 /ChKLnjfSaPsDfyP4sFkeAOTl40 Fig 5c ------- COMMENT: 3e4ec1257970269c 24 qZ3pPKr3SNNmCRqntNgHpV5N9Gw (comment: CHECK bet this is a term Val hates :p) ------- COMMENT: 3e4ec1257970269c 25 gax+5vOr1u+OrlZRL638YEnFy8s Figure 5a ------- COMMENT: 3e4ec1257970269c 26 1Sm0+hKUZ/gEPwKD9fyd/ro8PvA Figure 5d ------- COMMENT: 3e4ec1257970269c 27 1Sm0+hKUZ/gEPwKD9fyd/ro8PvA Figure 5d ------- COMMENT: 3e4ec1257970269c 28 1Sm0+hKUZ/gEPwKD9fyd/ro8PvA Figure 5d ------- COMMENT: 3e4ec1257970269c 30 8G6+j/Mkk6lmbSbCeunxDRKglvY Figure 7 ------- COMMENT: 3e4ec1257970269c 31 8G6+j/Mkk6lmbSbCeunxDRKglvY Figure 7 ------- COMMENT: 3e4ec1257970269c 32 IgFR51rw4m0tmLkwvZV99Nun2ig (comment: chromatin binding shown, and regulation of transcription shown. no evidence for dna binding BUT later paper by akayama and Toda state that " Ams2 directly binds a consensus "AACCCT-box" that exists in the 5' franking regions of these histone genes." and says that ams2 is sole responsible TF + cites this paper) ------- COMMENT: 3e62111aa45321c4 6 1u7nM0Z38WSZXhibQCysruAtIZs (comment: western but we know this happens and I wanted to capture the extension) ------- COMMENT: 3e62111aa45321c4 19 5d4EJ3/XXAYJehrdv2wUgNbbhzc (comment: CHECK salt stress) ------- COMMENT: 3e62111aa45321c4 20 5d4EJ3/XXAYJehrdv2wUgNbbhzc (comment: CHECK salt stress) ------- COMMENT: 3e62111aa45321c4 21 5d4EJ3/XXAYJehrdv2wUgNbbhzc (comment: CHECK salt stress) ------- COMMENT: 3e62111aa45321c4 22 5d4EJ3/XXAYJehrdv2wUgNbbhzc (comment: CHECK salt stress) ------- COMMENT: 3e62111aa45321c4 42 1u7nM0Z38WSZXhibQCysruAtIZs (comment: western but we know this happens and I wanted to capture the extension) ------- COMMENT: 3e7e104742b500a5 1 PxGYNeSxrV5PD+XotSQQNBxfnSo fig 1a ------- COMMENT: 3e7e104742b500a5 2 PxGYNeSxrV5PD+XotSQQNBxfnSo fig 1a ------- COMMENT: 3e7e104742b500a5 3 PxGYNeSxrV5PD+XotSQQNBxfnSo fig 1a ------- COMMENT: 3e7e104742b500a5 4 V8aY9cMSuflWPDnZXK1Ej8D/EEk fig 1b ------- COMMENT: 3e7e104742b500a5 5 V8aY9cMSuflWPDnZXK1Ej8D/EEk fig 1b ------- COMMENT: 3e7e104742b500a5 6 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: 3e7e104742b500a5 7 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: 3e7e104742b500a5 8 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: 3e7e104742b500a5 9 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: 3e7e104742b500a5 10 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: 3e7e104742b500a5 11 DoiKRO0bhQtBxgHeZUF1PeCk+Dk (comment: I don't understand the chemistry well enough to know how the HPLC shows this but I think this is enough evidence?) ------- COMMENT: 3e87e38190d31d91 14 jWja6fN67p6mRly/TKY4Y6NVjvU fig 4E ------- COMMENT: 3e87e38190d31d91 15 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 3e87e38190d31d91 16 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 3ee491206526745d 17 Jn/GC8uhWtrHYrTJPWuT20Yghbs (comment: same severity when crossed with wild type or shk1delta) ------- COMMENT: 3ee491206526745d 19 sPMM9MeNHoHSvDzNQlEXIGd1tBo (comment: when crossed with partner overexpressing shk1-deltaN; normal in cross with wild type) ------- COMMENT: 3ee491206526745d 21 z04Lqyfsih9v14eqShmEnemNQ9g (comment: when crossed with shk1delta overexpressing shk2+ or wild type) ------- COMMENT: 3f3648af903ea59c 5 EFr/HrtY5necoLIcWxIMO3C+MIg (comment: CHECK RIDD? - there is no RIDD term in GO, Val wants to wait with this) ------- COMMENT: 3f3648af903ea59c 12 GO0on9eWqSwyQ49OB+IGIj7aSQU (comment: ire1 breaks down mRNAs during ER stress, however bip1 is unusual in that ire1 cleavage stabilizes it) ------- COMMENT: 3f5875a2c3abe932 2 K/zwfWaJnoIjcINxJ0ii0h08st8 (comment: CHECK flocculation inhibited by galactose) ------- COMMENT: 3fc0a58cd1accb51 10 c6DUWc1yD+OZzhLiJnbyQ7+rgx8 ------- COMMENT: 3fc0a58cd1accb51 11 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig 2 ------- COMMENT: 3fce956824b89a65 3 YIiduypr245N9E02twiqS8PhqZc fig 1C ------- COMMENT: 3fce956824b89a65 4 cQlXXiy5heAR4XfJhVpMhH+DlOw fig 2A,2C,2D ------- COMMENT: 3fce956824b89a65 5 mO1Wr1ZkxZ9k9EWNTHl4McCB6Yc fig 2A,2D ------- COMMENT: 3fce956824b89a65 7 mO1Wr1ZkxZ9k9EWNTHl4McCB6Yc fig 2A,2D ------- COMMENT: 3fce956824b89a65 8 cQlXXiy5heAR4XfJhVpMhH+DlOw fig 2A,2C,2D ------- COMMENT: 3fce956824b89a65 10 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 3fce956824b89a65 11 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 3fce956824b89a65 12 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 3fce956824b89a65 13 RvAb581Mb3sNYdPYmy6tPIgGZRY figure 3A,3C ------- COMMENT: 3fce956824b89a65 14 RvAb581Mb3sNYdPYmy6tPIgGZRY figure 3A,3C ------- COMMENT: 3fce956824b89a65 15 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 3fce956824b89a65 16 zBl1Tl2ZGnMUqrgdOpeHRP27VCQ Figure 3 (comment: CHECK fypo/issues/2830) ------- COMMENT: 3fce956824b89a65 17 zBl1Tl2ZGnMUqrgdOpeHRP27VCQ Figure 3 (comment: CHECK fypo/issues/2830) ------- COMMENT: 3fce956824b89a65 18 koYlGh6LjCvS1ly6S4FQFXDPuY0 Figure 3B (comment: CHECK fypo/issues/2830) ------- COMMENT: 3fce956824b89a65 19 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 3fce956824b89a65 20 Jlk1V51+Ro3/iw8g0EtgXjlKa3U fig 5A ------- COMMENT: 3fce956824b89a65 21 Jlk1V51+Ro3/iw8g0EtgXjlKa3U fig 5A ------- COMMENT: 3fce956824b89a65 22 8N5pCuJDvQ4tU27Tb2MYBaI8aOw fig 5B ------- COMMENT: 3fce956824b89a65 24 MTyEGP4m3aCw3xo9QYvULYrAg5k fig 6B ------- COMMENT: 3fce956824b89a65 28 srNPEOHUR5maeOFJvV9hJaeBsr4 fig 6C ------- COMMENT: 40024707aa700ccb 6 NX6v/toclXh9222PH8RUlVRwEAI data not shown ------- COMMENT: 40033bc053698144 2 okkEB/Tw4KiG0NXU9V0K/Hw4Wn4 (comment: CHECK heterologous complemetation of S. c HMO1) ------- COMMENT: 4069663dbbd3273e 1 C6FWbNnQayq404bdthnSUNdsAOA Fig 3C (comment: CHECK at metaphase/anaphase transiton) ------- COMMENT: 4069663dbbd3273e 3 7IzPVIuZhuKqIVjB4l/cExHiwb8 fig 2D ------- COMMENT: 4069663dbbd3273e 4 2MB8zuAd/+VpKsVSPJNvOag+ul0 (comment: medium level of mph1 OEX (high is lethal)) ------- COMMENT: 4069663dbbd3273e 6 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: 4069663dbbd3273e 7 //QleQcD3kkWGdAJo2oN7LSuYAA fig 4B ------- COMMENT: 4069663dbbd3273e 8 //QleQcD3kkWGdAJo2oN7LSuYAA fig 4B ------- COMMENT: 4069663dbbd3273e 9 //QleQcD3kkWGdAJo2oN7LSuYAA fig 4B ------- COMMENT: 4069663dbbd3273e 10 GDfCltxaFGUhT96L8ZXMYXaCTj4 Fig. 4D ------- COMMENT: 4069663dbbd3273e 11 C6FWbNnQayq404bdthnSUNdsAOA Fig 3C (comment: CHECK at metaphase/anaphase transiton) ------- COMMENT: 4069663dbbd3273e 12 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 4069663dbbd3273e 13 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 4069663dbbd3273e 14 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 4069663dbbd3273e 15 zrUdmdMbY3RiR//l69j44cqXKEI dph1∆ cells were not hypersensitive to TBZ, compared to wild-type cells (Fig. 6C) ------- COMMENT: 40950ae0da782908 1 R80gUxZtTrfM7sOH09i/bsnS5D4 (comment: CONDITION 5 J/m2 UV) figure 1B, figure 3D, figure S6 ------- COMMENT: 40950ae0da782908 2 Char7QiL7cLP21E5L8SN5f3BU1I (comment: CONDITION 5 J/m2 UV) Fig 1A, Fig. S2 ------- COMMENT: 40950ae0da782908 3 OrvGRWNpuPs7meHXIQ+Bh04Ypqg (comment: CONDITION 5 J/m2 UV) Fig. 1B ------- COMMENT: 40950ae0da782908 4 DFHmcSr2JR/zsXXwjDL2/6qSDew (comment: CONDITION 5 J/m2 UV) Fig. 1B, Fig. 3E ------- COMMENT: 40950ae0da782908 5 GtzaeqRBPhsAXRBaNIQaiE1e5VA (comment: CONDITION 5 or 10 J/m2 UV) Fig. 3I, Fig. 4A, Fig. S6 ------- COMMENT: 40950ae0da782908 6 GtzaeqRBPhsAXRBaNIQaiE1e5VA (comment: CONDITION 5 or 10 J/m2 UV) Fig. 3I, Fig. 4A, Fig. S6 ------- COMMENT: 40950ae0da782908 8 GtzaeqRBPhsAXRBaNIQaiE1e5VA (comment: CONDITION 5 or 10 J/m2 UV) Fig. 3I, Fig. 4A, Fig. S6 ------- COMMENT: 40950ae0da782908 10 T/rdQ8c9kEeCZg/EwrpG8HOmKOQ (comment: CONDITION 5 J/m2 UV) Fig. 3C ------- COMMENT: 40950ae0da782908 11 GtzaeqRBPhsAXRBaNIQaiE1e5VA (comment: CONDITION 5 or 10 J/m2 UV) Fig. 3I, Fig. 4A, Fig. S6 ------- COMMENT: 40950ae0da782908 12 6uysSZEaNn0Ri7W2rmLNCc+cdIU (comment: CONDITION 2 or 5 J/m2 UV) Fig. 3F, Fig. S6 ------- COMMENT: 40950ae0da782908 13 b0+VEKoNEnKHeZWREuqZhyqb2qA (comment: CONDITION 5 J/m2 UV) Fig. 3D ------- COMMENT: 40950ae0da782908 14 90dPfnwDa9NbDynsy4Qc7vIqzLA (comment: CONDITION 5 J/m2 UV) Fig. 3E ------- COMMENT: 40950ae0da782908 15 L2b0fGPd0fKlD2Z/ZiokMu7dbjo (comment: CONDITION 5 J/m2 UV) Fig. 3I, Fig. 4A, Fig. S6 ------- COMMENT: 40950ae0da782908 16 L2b0fGPd0fKlD2Z/ZiokMu7dbjo (comment: CONDITION 5 J/m2 UV) Fig. 3I, Fig. 4A, Fig. S6 ------- COMMENT: 40950ae0da782908 17 JAaWBGkU9TeZRIfrNXfh5l2BXOU (comment: CONDITION 5 J/m2 UV) Fig. 3B ------- COMMENT: 40950ae0da782908 18 T/rdQ8c9kEeCZg/EwrpG8HOmKOQ (comment: CONDITION 5 J/m2 UV) Fig. 3C ------- COMMENT: 40950ae0da782908 19 6uysSZEaNn0Ri7W2rmLNCc+cdIU (comment: CONDITION 2 or 5 J/m2 UV) Fig. 3F, Fig. S6 ------- COMMENT: 40950ae0da782908 23 Kds1O2tU/F9QN8Ma7wkn5BLfSqE (comment: CONDITION 5 J/m2 UV) duration is similar to eso1-D147N alone (see Fig. 3H) ------- COMMENT: 40950ae0da782908 24 ffQ8m0c58l3y6QFNZ1TFLMiAPtI (comment: CONDITION 5 J/m2 UV) duration is similar to rad8delta or rhp18delta single mutants (see Fig. 3G) ------- COMMENT: 40950ae0da782908 25 DYtflGi9haZx4FL0Hr7uw7t451Q (comment: CONDITION 5 J/m2 UV) Delay is greater than rad51delta alone (see Fig. S7) ------- COMMENT: 40950ae0da782908 26 LMLQUTYfDj6HDmLkYes+ja0TIgY (comment: CONDITION 25 J/m2 UV) delay is greater than rad51delta alone (Fig. 5A) ------- COMMENT: 40950ae0da782908 27 M03tyKwul2iC4FfFhjl5+GdhsTk (comment: CONDITION 5 or 10 J/m2 UV) similar sensitivity to rev1delta and rev3delta single mutants (Fig. 3I, Fig. 4A, Fig. S6) ------- COMMENT: 40950ae0da782908 28 Uy51muqRvB8Il3nGlfM0nbzQJ3E (comment: CONDITION 5 J/m2 UV) similar sensitivity to eso1-D147N single mutant (Fig. 3H) ------- COMMENT: 40950ae0da782908 29 mml/HPpo1buI3dBL2MLZIZBSmNQ (comment: CONDITION 5 J/m2 UV) sensitivity similar to rad8delta and rhp18delta single mutants (Fig. 3G) ------- COMMENT: 40950ae0da782908 30 ECMGIqueYl+bpWwe8JvfwmDikjU (comment: CONDITION 5 J/m2 UV) Fig. S3 ------- COMMENT: 40950ae0da782908 31 1lpvYiIwU9uBL+h/pSlp0j867IE (comment: CONDITION 5 J/m2 UV) Sensitivity is greater than rad51delta or eso1-D147N single mutants (see Fig. S7) ------- COMMENT: 40a3b7f45b30fa7f 1 IiFK0wcMaEoGCmD1GHVamZWSeoM A reaction time course revealed that most dsDNA binding occurred within the first 15 min of incubation, whereas dsDNA binding by Walker A ATPase motif mutant condensin was not stimulated by ATP addition (Figures S1B and S1C). These results show that recombinant fission yeast condensin binds to DNA in an ATP-stimulated, high-salt-resistant manner, characteristic of topological DNA interactions by SMC complexes. Although an intact supercoiled plasmid remained stably bound to condensin in the bead fraction, linearized dsDNA was released into the su- pernatant. This experiment confirms that ATP-dependent con- densin loading results in a topological DNA interaction. ------- COMMENT: 40a3b7f45b30fa7f 2 XBRxXEHSWFaJWcNSD1o8s62+5JQ DNA-DNA tethering activity, sequential topological entrapment: sequential topological entrapment of two or more DNAs, A DNA tethering activity where a protein complex encircles two or more DNA molecules, one at a time, with its loose fitting ring. ------- COMMENT: 40a3b7f45b30fa7f 3 DBnJlO1zPpEc4bZMzAdXv+7hEcY (comment: author suggested) double-stranded DNA gripping or clamping A DNA binding activity, stimulated by the binding of a non-hydrolylsable ATP analogue, where a ATPase protein complex tightly grips a stretch of double-stranded DNA with some or all of its subunits. Such a conformation is usually interpretted as the intermediate state before the ATP hydrolysis by the protein complex. ------- COMMENT: 40a3b7f45b30fa7f 4 f8DN3vZ2FT+bQnNYWCXBr1iTvwI A reaction time course revealed that most dsDNA binding occurred within the first 15 min of incubation, whereas dsDNA binding by Walker A ATPase motif mutant condensin was not stimulated by ATP addition (Figures S1B and S1C). These results show that recombinant fission yeast condensin binds to DNA in an ATP-stimulated, high-salt-resistant manner, characteristic of topological DNA interactions by SMC complexes. Three topologically closed dsDNA sub- strates—supercoiled, relaxed circular, and nicked circular— were all recovered with similar efficiency (Figure 1B). Condensin also bound, albeit less efficiently, circular single-stranded DNA (ssDNA). By contrast, we observed no detectable recovery of linear dsDNA, consistent with a topological condensin-DNA interaction. ------- COMMENT: 40a3b7f45b30fa7f 5 qHvYrC8mruE80qOB8B8ZubTOOzs A reaction time course revealed that most dsDNA binding occurred within the first 15 min of incubation, whereas dsDNA binding by Walker A ATPase motif mutant condensin was not stimulated by ATP addition (Figures S1B and S1C). These results show that recombinant fission yeast condensin binds to DNA in an ATP-stimulated, high-salt-resistant manner, characteristic of topological DNA interactions by SMC complexes. ------- COMMENT: 40a3b7f45b30fa7f 6 qHvYrC8mruE80qOB8B8ZubTOOzs A reaction time course revealed that most dsDNA binding occurred within the first 15 min of incubation, whereas dsDNA binding by Walker A ATPase motif mutant condensin was not stimulated by ATP addition (Figures S1B and S1C). These results show that recombinant fission yeast condensin binds to DNA in an ATP-stimulated, high-salt-resistant manner, characteristic of topological DNA interactions by SMC complexes. ------- COMMENT: 40a3b7f45b30fa7f 7 FlYDWCTdr26xHF4Xr/FL7QW7YYE sequential topological entrapment of two or more DNAs, A DNA tethering activity where a protein complex encircles two or more DNA molecules, one at a time, with its loose fitting ring. ------- COMMENT: 40a3b7f45b30fa7f 8 FlYDWCTdr26xHF4Xr/FL7QW7YYE sequential topological entrapment of two or more DNAs, A DNA tethering activity where a protein complex encircles two or more DNA molecules, one at a time, with its loose fitting ring. ------- COMMENT: 40a3b7f45b30fa7f 9 FlYDWCTdr26xHF4Xr/FL7QW7YYE sequential topological entrapment of two or more DNAs, A DNA tethering activity where a protein complex encircles two or more DNA molecules, one at a time, with its loose fitting ring. ------- COMMENT: 40a3b7f45b30fa7f 10 FlYDWCTdr26xHF4Xr/FL7QW7YYE sequential topological entrapment of two or more DNAs, A DNA tethering activity where a protein complex encircles two or more DNA molecules, one at a time, with its loose fitting ring. ------- COMMENT: 40a3b7f45b30fa7f 11 qHvYrC8mruE80qOB8B8ZubTOOzs A reaction time course revealed that most dsDNA binding occurred within the first 15 min of incubation, whereas dsDNA binding by Walker A ATPase motif mutant condensin was not stimulated by ATP addition (Figures S1B and S1C). These results show that recombinant fission yeast condensin binds to DNA in an ATP-stimulated, high-salt-resistant manner, characteristic of topological DNA interactions by SMC complexes. ------- COMMENT: 40b664513ff3dccd 3 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 40b664513ff3dccd 4 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 40b664513ff3dccd 5 94/mmQgq9xWJt8s8J4W0d+qjd+c (comment: TEL2L only) ------- COMMENT: 40b664513ff3dccd 6 94/mmQgq9xWJt8s8J4W0d+qjd+c (comment: TEL2L only) ------- COMMENT: 40b664513ff3dccd 45 94/mmQgq9xWJt8s8J4W0d+qjd+c (comment: TEL2L only() ------- COMMENT: 40b664513ff3dccd 49 iTywpV1sv7cO0s4EFKspSkBQG/E (comment: also from localization and phenotypes) ------- COMMENT: 40b664513ff3dccd 50 iTywpV1sv7cO0s4EFKspSkBQG/E (comment: also from localization and phenotypes) ------- COMMENT: 40fd88a127db667a 6 W5SoSxJxkYMN9HiSpdJsBdlR+7A (comment: assayed in strain with RTS1 replication fork barrier inserted near ori3006/7) ------- COMMENT: 40fd88a127db667a 9 W5SoSxJxkYMN9HiSpdJsBdlR+7A (comment: assayed in strain with RTS1 replication fork barrier inserted near ori3006/7) ------- COMMENT: 40fd88a127db667a 10 6yW4DXyLT/Z2H+Ep1hvPl9ZOLr0 (comment: assayed by PCR in strain with RTS1 replication fork barrier inserted near ori3006/7) ------- COMMENT: 40fd88a127db667a 12 6yW4DXyLT/Z2H+Ep1hvPl9ZOLr0 (comment: assayed by PCR in strain with RTS1 replication fork barrier inserted near ori3006/7) ------- COMMENT: 40fd88a127db667a 18 6yW4DXyLT/Z2H+Ep1hvPl9ZOLr0 (comment: assayed by PCR in strain with RTS1 replication fork barrier inserted near ori3006/7) ------- COMMENT: 410ec2f68d834d33 1 oL8BWQ1Y7RbaWLVWm7uAlSVGjIU Both mutant proteins were expressed at near wild-type levels, suggesting that these acidic residues are not required for protein stability (data not shown). ------- COMMENT: 410ec2f68d834d33 2 oL8BWQ1Y7RbaWLVWm7uAlSVGjIU Both mutant proteins were expressed at near wild-type levels, suggesting that these acidic residues are not required for protein stability (data not shown). ------- COMMENT: 410ec2f68d834d33 3 1WMNKx7TlDHkhOYHMg2VLYs8x9Y (comment: increased length hererogeneity) ------- COMMENT: 410ec2f68d834d33 4 3rNLeQEPyuKpYKvI8Vsp/N1Q4j0 (comment: increased length hererogeneity) taz1-4A cells still exhibited extremely heterogeneous telomeres similar to taz1Δ and taz1-4R cells (Figure 1E). ------- COMMENT: 410ec2f68d834d33 5 1WMNKx7TlDHkhOYHMg2VLYs8x9Y (comment: increased length hererogeneity) ------- COMMENT: 410ec2f68d834d33 12 L/BLKTIXNvQi435jBnhTek1+EFw While wild-type Taz1 bound to DNA with an equilibrium dissociation constant (Kd) of ~600 nM (Figure 1I), the L445R mutation caused a 10-fold decrease in DNA binding with a Kd of ~7 μM (Figure 1I), suggesting that Taz1 homodimerization is required for its efficient association with the telomeric DNA in vitro. ------- COMMENT: 410ec2f68d834d33 13 Y245sX8pn5NdegqzVUYLMwOPeIU The L445R mutation caused a 10-fold decrease in DNA binding with a Kd of ~7 μM (Figure 1I), suggesting that Taz1 homodimerization is ------- COMMENT: 410ec2f68d834d33 14 66rc+iOxMzEuBaTI6K+ePolekNU Mutant proteins were expressed at a comparable level as wild-type Taz1 (data not shown). ------- COMMENT: 410ec2f68d834d33 15 66rc+iOxMzEuBaTI6K+ePolekNU Mutant proteins were expressed at a comparable level as wild-type Taz1 (data not shown). ------- COMMENT: 410ec2f68d834d33 16 +7nVj523R43u/zdaiKIhsyxx3xI (comment: increased length hererogeneity*****************) Indeed, yeast cells expressing the L431R and L445R mutants exhibited significant loss of function in telomere length regulation and showed long and highly heterogenous telomeres that were as severe as that in taz1Δ cells (Figure 1K) ------- COMMENT: 410ec2f68d834d33 17 +7nVj523R43u/zdaiKIhsyxx3xI (comment: increased length hererogeneity*****************) Indeed, yeast cells expressing the L431R and L445R mutants exhibited significant loss of function in telomere length regulation and showed long and highly heterogenous telomeres that were as severe as that in taz1Δ cells (Figure 1K) ------- COMMENT: 413cccbc0ecd978c 1 mKeFX4iRDQdF01NBBay+l8ZHxhk (comment: NMR + substrate) ------- COMMENT: 416865874f3e468b 1 kjFC64nLJJwhYUraFlvAHhDX9pU Unexpectedly, Mag2 showed no DNA glycosylase activity for alkylated bases, even at very high enzyme concentration and under different assay conditions (titration of NaCl, Mg2+, ATP; data not shown). Further, heterologous expression of Mag2 did neither rescue the extreme alkylation sensitive phenotype of an E. coli mutant lacking the two 3mA DNA glycosylases AlkA and Tag, nor the corresponding Saccharomyces cerevisiae mag1 deletion mutant (data not shown). Mag2 was assayed for activity towards a variety of different base lesions which are known substrates for other DNA glycosylases including alkylated, oxidized and deaminated bases, base mismatches and AP sites; however no enzymatic activity was observed for any of the lesions tested (Table S1) Crystal Structure of Mag2 in Complex With DNA Reveals Novel Non-Enzymatic AP Site Recognition and DNA Sculpting ------- COMMENT: 416865874f3e468b 3 GSDoKVHrCsiUO6zYP0Vg8a8VAAQ fig 1D ------- COMMENT: 416865874f3e468b 4 GSDoKVHrCsiUO6zYP0Vg8a8VAAQ fig 1D ------- COMMENT: 416865874f3e468b 5 GSDoKVHrCsiUO6zYP0Vg8a8VAAQ fig 1D ------- COMMENT: 416865874f3e468b 6 vVCZMclUMPArmweb1xEZXu4rue0 Supp S1 Also the MMS sensitivity of the nth1 − mutant was suppressed by the deletion of mag1 but not mag2 (Figure S1), which contrasts previous results (Kanamitsu et al., 2007). We do not know the reason for this discrepancy, but it could be because of different strain backgrounds, as the strains used by Kanamisu and co-workers tolerate much higher MMS doses (0.03% versus 0.007% in our experiments) ------- COMMENT: 416865874f3e468b 7 jc6emhZJfAqAHqJgg6kIdUd1EM8 multiple experiments ------- COMMENT: 416865874f3e468b 8 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 416865874f3e468b 9 2UfC5s+UPFvE1BN6dOUhHCFcEPM Mag2 binds stronger to the abasic oligonucleotide than to non-damaged DNA (Figure 2), with a dissociation rate constant more than 15 times higher for non-damaged DNA (kd 26 × 10−3 s −1) as compared to DNA containing the AP site analogue tetrahydrofuran (THF) (kd 1.6 × 10−3 s−1). Mag2 injected on sensor chips coated with oligonucleotides containing a single ethenoadenine or 8-oxoguanine lesion showed the same resonance levels as non-damaged DNA (data not shown), demonstrating that Mag2 preferentially binds to AP sites in DNA. ------- COMMENT: 4172bbc99aeb070b 3 RVbgqS4KYWpUOs0Qifx2FCEWqXg (comment: unphosphorylated form of tif211) ------- COMMENT: 4172bbc99aeb070b 4 O+/x8c3FH3WASyx4aCK122YLTok ((comment: unphosphorylated form of tif211) inhibited by stress-inducedphosphorylation of Ser51 in the a subunit of eIF2(tif211) ------- COMMENT: 41cf39ea6dc64d5a 12 NrEGqlSMdNk0GHmHmb/YRgyJ6Rc (comment: pNBg was used) ------- COMMENT: 41cf39ea6dc64d5a 13 LynpCwJZHc4OuDidzX1CXrDaR6k (comment: cen2-lacO) ------- COMMENT: 41f753fbed9bec67 1 k6rftAnSC1cgx7YEKgzVj83UK3o ((comment: included because different/new method) To observe the SPB in living cells, GFP-tagged Sad1 (designated hereafter Sad1–GFP) was expressed and found to be bound to the SPB throughout the cell cycle (Figure 1A), identical to immunolocalization data (Ha- gan and Yanagida, 1995) ------- COMMENT: 41f753fbed9bec67 2 9JDiuSEjIGMPD6yjD2CWIGayVjg ((comment: included because different/new method) To observe the SPB in living cells, GFP-tagged Sad1 (designated hereafter Sad1–GFP) was expressed and found to be bound to the SPB throughout the cell cycle (Figure 1A), identical to immunolocalization data (Ha- gan and Yanagida, 1995) ------- COMMENT: 41f753fbed9bec67 5 lJDU/1ILMCt1/6QAylt8FVddRhs A striking feature in dis1 mutant cells was that the back-and-forth cen1 DNA movements seen in phase 2 of wild-type cells were entirely absent. After spindle elongation (the SPB distance, ô°†8 ô°ˆm), the cen1 signals were fused again and moved to one of the SPBs. Such prolonged centromere splitting while the spindle was elongating was never seen in wild-type or any of the other mutant cells examined so far. ------- COMMENT: 42005ff3ae0fa290 1 uWv3ZTih6JwxY6VRIoexrc3zflQ Atg44 binds to lipid membranes in vitro (Figures 5A and 5E), and the cryo-EM and HS-AFM analyses (Figures 5F–5I) suggest that Atg44 tends to bind to lipid membranes with high curvature. ------- COMMENT: 42005ff3ae0fa290 2 UuxpFkXH+fWwCjgssumChdgyQ5E Unexpectedly, we noticed that addition of Sp-Atg44 caused fragmentation of lipid bilayers on the mica (compare Figures 7B and 7C), and the fragmented lipid bilayers abundant with Sp-Atg44 underwent division and fusion (Figure 7D; Video S6). These observations suggest that Sp-Atg44 has the ability to cause membrane fragility through physical interaction. ------- COMMENT: 42005ff3ae0fa290 5 I4g9zZg+m2RDuSs60G/n/QIZSN8 Based on these results, we conclude that Atg44 localizes in the IMS and is not a transmembrane protein. ------- COMMENT: 42005ff3ae0fa290 6 2qfsPSoa+8OVwm/0w4WA4VJ77mU In atg44D cells, mitophagy was completely blocked similarly to cells lacking Atg1, a core autophagy protein (Figures 1A and S1B). ------- COMMENT: 42005ff3ae0fa290 7 62fl0Z/6XAegPZHw0kLkS9GjX4I Similarly, loss of Atg44 in S. pombe affected mitochondrial morphology; some of the Sp-atg44D cells showed spherically enlarged mitochondria like Sp-dnm1D cells (Figure 3D) ------- COMMENT: 42005ff3ae0fa290 8 lm8D2mDN/OCveftexhQTu00UwG8 Loss of Atg44 in either S. pombe or S. cerevisiae did not or only marginally affected non-selective macroautophagy, as measured by GFP/RFP processing (Figures S1C, S1D, and S1F) or the Pho8D60 assay (Figure 1E), or other types of selective autophagy including the Cvt pathway that delivers the precursor form of the hydrolase aminopeptidase I to the vacuole (Figure S1G), endoplasmic reticulum-phagy/reticulophagy (Figures S1E and S1H), and pexophagy (Figure S1I), suggesting that Atg44 is specifically required for mitophagy. ------- COMMENT: 42005ff3ae0fa290 9 kDPre+UvoD+Ir3Z53XwTuY4HEKM Overexpression of Atg44 in both species caused mitochondrial fragmentation not only in wild-type cells but also in Dnm1-deficient cells (Figures 3E and 3F). ------- COMMENT: 42005ff3ae0fa290 10 xBKASsirt83KqGP1npmFTEOVJRY As expected, in S. pombe atg44D cellslacking Mgm1, some of the mitochondria became fragmented and mitophagy was partially rescued (Figures 4A and S3F). ------- COMMENT: 420a5c4d2fd025ad 1 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 420a5c4d2fd025ad 2 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 420a5c4d2fd025ad 3 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 420a5c4d2fd025ad 4 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 420a5c4d2fd025ad 5 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 420a5c4d2fd025ad 6 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 420a5c4d2fd025ad 7 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 420a5c4d2fd025ad 8 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 420a5c4d2fd025ad 9 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 420a5c4d2fd025ad 10 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 420a5c4d2fd025ad 11 PDxxJFf8kYfG+o5TIMamYzM5/P4 Fig. 5 (comment: Dephosph form) ------- COMMENT: 420a5c4d2fd025ad 12 PDxxJFf8kYfG+o5TIMamYzM5/P4 Fig. 5 (comment: Dephosph form) ------- COMMENT: 420a5c4d2fd025ad 13 er8XoLRFoc2QERgUmAZGnlpk7AQ Fig. 5 (comment: Phosph form) ((comment: From other publications, we know bona-fide that the localisation observed here is the kinetochore, but here they study how it changes) ------- COMMENT: 420a5c4d2fd025ad 14 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 420a5c4d2fd025ad 18 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 420a5c4d2fd025ad 19 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 420a5c4d2fd025ad 20 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 420a5c4d2fd025ad 21 gpB2fw0YMjGnCZjWwsLyEKEyi0Y Fig. 5 Supp 3 ------- COMMENT: 420a5c4d2fd025ad 22 gpB2fw0YMjGnCZjWwsLyEKEyi0Y Fig. 5 Supp 3 ------- COMMENT: 420a5c4d2fd025ad 23 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 420a5c4d2fd025ad 25 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 420a5c4d2fd025ad 26 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 4228fd893e7a1a88 1 oHUUAt7R1UcZH5Yz0OZqJ0fO60M Fig S7 ------- COMMENT: 4228fd893e7a1a88 2 oHUUAt7R1UcZH5Yz0OZqJ0fO60M Fig S7 ------- COMMENT: 4228fd893e7a1a88 3 oHUUAt7R1UcZH5Yz0OZqJ0fO60M Fig S7 ------- COMMENT: 4228fd893e7a1a88 4 oHUUAt7R1UcZH5Yz0OZqJ0fO60M Fig S7 ------- COMMENT: 4228fd893e7a1a88 5 oHUUAt7R1UcZH5Yz0OZqJ0fO60M Fig S7 ------- COMMENT: 4228fd893e7a1a88 6 oHUUAt7R1UcZH5Yz0OZqJ0fO60M Fig S7 ------- COMMENT: 4228fd893e7a1a88 7 RT474W3Qr/KqeSNKNt+cFHOhxto Fig S6 ------- COMMENT: 4228fd893e7a1a88 8 Vm3MArAwrSeFHopDWS3cqvFzigs Fig S6, new term suggested ------- COMMENT: 4228fd893e7a1a88 9 ncbZD8ZqO4fN97WbwMXimXW399A Fig 12B ------- COMMENT: 4228fd893e7a1a88 10 ncbZD8ZqO4fN97WbwMXimXW399A Fig 12B ------- COMMENT: 4228fd893e7a1a88 11 ncbZD8ZqO4fN97WbwMXimXW399A Fig 12B ------- COMMENT: 4228fd893e7a1a88 12 70kDwSNd8eg6oAo1xzHz0xMw7n8 Fig S5A ------- COMMENT: 4228fd893e7a1a88 13 70kDwSNd8eg6oAo1xzHz0xMw7n8 Fig S5A ------- COMMENT: 4228fd893e7a1a88 14 Q68TPp0Xdpyrj/d3mT6Zm2zGNyo Fig S5B ------- COMMENT: 4228fd893e7a1a88 15 Q68TPp0Xdpyrj/d3mT6Zm2zGNyo Fig S5B ------- COMMENT: 4228fd893e7a1a88 16 hQeySTZ7FeHk3Zi/t7AmBl0InUQ Fig S4 ------- COMMENT: 4228fd893e7a1a88 17 hQeySTZ7FeHk3Zi/t7AmBl0InUQ Fig S4 ------- COMMENT: 4228fd893e7a1a88 18 hQeySTZ7FeHk3Zi/t7AmBl0InUQ Fig S4 ------- COMMENT: 4228fd893e7a1a88 19 hQeySTZ7FeHk3Zi/t7AmBl0InUQ Fig S4 ------- COMMENT: 4228fd893e7a1a88 21 tEKPG4SG06Sd+MBgYUZdTXf2msI Fig S3 ------- COMMENT: 4228fd893e7a1a88 22 tEKPG4SG06Sd+MBgYUZdTXf2msI Fig S3 ------- COMMENT: 4228fd893e7a1a88 23 tEKPG4SG06Sd+MBgYUZdTXf2msI Fig S3 ------- COMMENT: 4228fd893e7a1a88 24 tEKPG4SG06Sd+MBgYUZdTXf2msI Fig S3 ------- COMMENT: 4228fd893e7a1a88 25 JzyBKOIiyuH2U/GwaoWxC6/fJhQ Fig S2 ------- COMMENT: 4228fd893e7a1a88 26 oLsS5bWkKAW72/VpGEDmN/P+1lo Fig 13 ------- COMMENT: 4228fd893e7a1a88 27 13FSZjggfoRKIlYb0GMzFnZ/t0o Fig 12A ------- COMMENT: 4228fd893e7a1a88 28 oLsS5bWkKAW72/VpGEDmN/P+1lo Fig 13 ------- COMMENT: 4228fd893e7a1a88 29 oLsS5bWkKAW72/VpGEDmN/P+1lo Fig 13 ------- COMMENT: 4228fd893e7a1a88 30 oLsS5bWkKAW72/VpGEDmN/P+1lo Fig 13 ------- COMMENT: 4228fd893e7a1a88 31 CRBXmcV/mDBTwXjm3US9JLllppY Fig 13, new term suggested ------- COMMENT: 4228fd893e7a1a88 32 CRBXmcV/mDBTwXjm3US9JLllppY Fig 13, new term suggested ------- COMMENT: 4228fd893e7a1a88 33 CRBXmcV/mDBTwXjm3US9JLllppY Fig 13, new term suggested ------- COMMENT: 4228fd893e7a1a88 34 CRBXmcV/mDBTwXjm3US9JLllppY Fig 13, new term suggested ------- COMMENT: 4228fd893e7a1a88 35 CRBXmcV/mDBTwXjm3US9JLllppY Fig 13, new term suggested ------- COMMENT: 4228fd893e7a1a88 36 oLsS5bWkKAW72/VpGEDmN/P+1lo Fig 13 ------- COMMENT: 4228fd893e7a1a88 37 oLsS5bWkKAW72/VpGEDmN/P+1lo Fig 13 ------- COMMENT: 4228fd893e7a1a88 38 oLsS5bWkKAW72/VpGEDmN/P+1lo Fig 13 ------- COMMENT: 4228fd893e7a1a88 41 wM3dwVmmrXofb8tdqICj0tWQtUk Fig 11A ------- COMMENT: 4228fd893e7a1a88 42 wM3dwVmmrXofb8tdqICj0tWQtUk Fig 11A ------- COMMENT: 4228fd893e7a1a88 43 wM3dwVmmrXofb8tdqICj0tWQtUk Fig 11A ------- COMMENT: 4228fd893e7a1a88 44 wM3dwVmmrXofb8tdqICj0tWQtUk Fig 11A ------- COMMENT: 4228fd893e7a1a88 45 wM3dwVmmrXofb8tdqICj0tWQtUk Fig 11A ------- COMMENT: 4228fd893e7a1a88 46 wM3dwVmmrXofb8tdqICj0tWQtUk Fig 11A ------- COMMENT: 4228fd893e7a1a88 47 wM3dwVmmrXofb8tdqICj0tWQtUk Fig 11A ------- COMMENT: 4228fd893e7a1a88 48 wM3dwVmmrXofb8tdqICj0tWQtUk Fig 11A ------- COMMENT: 4228fd893e7a1a88 49 wM3dwVmmrXofb8tdqICj0tWQtUk Fig 11A ------- COMMENT: 4228fd893e7a1a88 50 LTwSVJ/H1RqUQjcxtqYiDEBHcic Fig 11B ------- COMMENT: 4228fd893e7a1a88 51 LTwSVJ/H1RqUQjcxtqYiDEBHcic Fig 11B ------- COMMENT: 4228fd893e7a1a88 52 LTwSVJ/H1RqUQjcxtqYiDEBHcic Fig 11B ------- COMMENT: 4228fd893e7a1a88 53 LTwSVJ/H1RqUQjcxtqYiDEBHcic Fig 11B ------- COMMENT: 4228fd893e7a1a88 54 LTwSVJ/H1RqUQjcxtqYiDEBHcic Fig 11B ------- COMMENT: 4228fd893e7a1a88 55 LTwSVJ/H1RqUQjcxtqYiDEBHcic Fig 11B ------- COMMENT: 4228fd893e7a1a88 56 LTwSVJ/H1RqUQjcxtqYiDEBHcic Fig 11B ------- COMMENT: 4228fd893e7a1a88 57 LTwSVJ/H1RqUQjcxtqYiDEBHcic Fig 11B ------- COMMENT: 4228fd893e7a1a88 58 XHGtdSgfGkdw2ECevFwBKNieYYc (comment: Described in Garg et al. PMID:33010152) ------- COMMENT: 4228fd893e7a1a88 59 XHGtdSgfGkdw2ECevFwBKNieYYc (comment: Described in Garg et al. PMID:33010152) ------- COMMENT: 4228fd893e7a1a88 60 IxBrvM9hK2WVcVuAwtPegLkI7So Fig 10A ------- COMMENT: 4228fd893e7a1a88 61 IxBrvM9hK2WVcVuAwtPegLkI7So Fig 10A ------- COMMENT: 4228fd893e7a1a88 62 IxBrvM9hK2WVcVuAwtPegLkI7So Fig 10A ------- COMMENT: 4228fd893e7a1a88 63 IxBrvM9hK2WVcVuAwtPegLkI7So Fig 10A ------- COMMENT: 4228fd893e7a1a88 64 IxBrvM9hK2WVcVuAwtPegLkI7So Fig 10A ------- COMMENT: 4228fd893e7a1a88 65 IxBrvM9hK2WVcVuAwtPegLkI7So Fig 10A ------- COMMENT: 4228fd893e7a1a88 66 IxBrvM9hK2WVcVuAwtPegLkI7So Fig 10A ------- COMMENT: 4228fd893e7a1a88 67 IxBrvM9hK2WVcVuAwtPegLkI7So Fig 10A ------- COMMENT: 4228fd893e7a1a88 68 IxBrvM9hK2WVcVuAwtPegLkI7So Fig 10A ------- COMMENT: 4228fd893e7a1a88 69 IxBrvM9hK2WVcVuAwtPegLkI7So Fig 10A ------- COMMENT: 4228fd893e7a1a88 70 IxBrvM9hK2WVcVuAwtPegLkI7So Fig 10A ------- COMMENT: 4228fd893e7a1a88 71 IxBrvM9hK2WVcVuAwtPegLkI7So Fig 10A ------- COMMENT: 4228fd893e7a1a88 72 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 4228fd893e7a1a88 73 QW1kG0ulsYrW4/LFoH/uyONU3KU Fig 10B ------- COMMENT: 4228fd893e7a1a88 74 QW1kG0ulsYrW4/LFoH/uyONU3KU Fig 10B ------- COMMENT: 4228fd893e7a1a88 75 QW1kG0ulsYrW4/LFoH/uyONU3KU Fig 10B ------- COMMENT: 4228fd893e7a1a88 76 QW1kG0ulsYrW4/LFoH/uyONU3KU Fig 10B ------- COMMENT: 4228fd893e7a1a88 77 QW1kG0ulsYrW4/LFoH/uyONU3KU Fig 10B ------- COMMENT: 4228fd893e7a1a88 78 QW1kG0ulsYrW4/LFoH/uyONU3KU Fig 10B ------- COMMENT: 4228fd893e7a1a88 79 QW1kG0ulsYrW4/LFoH/uyONU3KU Fig 10B ------- COMMENT: 4228fd893e7a1a88 80 QW1kG0ulsYrW4/LFoH/uyONU3KU Fig 10B ------- COMMENT: 4228fd893e7a1a88 81 QW1kG0ulsYrW4/LFoH/uyONU3KU Fig 10B ------- COMMENT: 4228fd893e7a1a88 82 QW1kG0ulsYrW4/LFoH/uyONU3KU Fig 10B ------- COMMENT: 4228fd893e7a1a88 83 QW1kG0ulsYrW4/LFoH/uyONU3KU Fig 10B ------- COMMENT: 4228fd893e7a1a88 84 QW1kG0ulsYrW4/LFoH/uyONU3KU Fig 10B ------- COMMENT: 4228fd893e7a1a88 85 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 4228fd893e7a1a88 86 EkDslmRI7jammyNy1lz+ADBpdh8 Fig 8A ------- COMMENT: 4228fd893e7a1a88 87 EkDslmRI7jammyNy1lz+ADBpdh8 Fig 8A ------- COMMENT: 4228fd893e7a1a88 88 suilSiYqGwnif972/vcDHVTaK0c Fig 8B ------- COMMENT: 4228fd893e7a1a88 89 suilSiYqGwnif972/vcDHVTaK0c Fig 8B ------- COMMENT: 4228fd893e7a1a88 90 tc3rJxZshAX+tmBq7BR9TkADO4o Fig 7A ------- COMMENT: 4228fd893e7a1a88 91 tc3rJxZshAX+tmBq7BR9TkADO4o Fig 7A ------- COMMENT: 4228fd893e7a1a88 92 tc3rJxZshAX+tmBq7BR9TkADO4o Fig 7A ------- COMMENT: 4228fd893e7a1a88 93 tc3rJxZshAX+tmBq7BR9TkADO4o Fig 7A ------- COMMENT: 4228fd893e7a1a88 94 kTvB5rbZANgdq/lfui571ZaBluI Fig 7B ------- COMMENT: 4228fd893e7a1a88 95 kTvB5rbZANgdq/lfui571ZaBluI Fig 7B ------- COMMENT: 4228fd893e7a1a88 96 kTvB5rbZANgdq/lfui571ZaBluI Fig 7B ------- COMMENT: 4228fd893e7a1a88 97 kTvB5rbZANgdq/lfui571ZaBluI Fig 7B ------- COMMENT: 4228fd893e7a1a88 98 kTvB5rbZANgdq/lfui571ZaBluI Fig 7B ------- COMMENT: 4228fd893e7a1a88 99 kTvB5rbZANgdq/lfui571ZaBluI Fig 7B ------- COMMENT: 4228fd893e7a1a88 100 tc3rJxZshAX+tmBq7BR9TkADO4o Fig 7A ------- COMMENT: 4228fd893e7a1a88 101 tc3rJxZshAX+tmBq7BR9TkADO4o Fig 7A ------- COMMENT: 4228fd893e7a1a88 102 tc3rJxZshAX+tmBq7BR9TkADO4o Fig 7A ------- COMMENT: 4228fd893e7a1a88 103 6ETquB6qf7XZAg8RDXUoPTp/g3Q Fig 6A ------- COMMENT: 4228fd893e7a1a88 104 6ETquB6qf7XZAg8RDXUoPTp/g3Q Fig 6A ------- COMMENT: 4228fd893e7a1a88 106 t06dwlfQjN9Tx1OFuUhULmgZu5c Fig 6B ------- COMMENT: 4228fd893e7a1a88 107 t06dwlfQjN9Tx1OFuUhULmgZu5c Fig 6B ------- COMMENT: 4228fd893e7a1a88 108 VFkskau1qxV6L9SVWfIqbfw500g Fig 5A ------- COMMENT: 4228fd893e7a1a88 109 VFkskau1qxV6L9SVWfIqbfw500g Fig 5A ------- COMMENT: 4228fd893e7a1a88 110 VFkskau1qxV6L9SVWfIqbfw500g Fig 5A ------- COMMENT: 4228fd893e7a1a88 111 VFkskau1qxV6L9SVWfIqbfw500g Fig 5A ------- COMMENT: 4228fd893e7a1a88 112 VFkskau1qxV6L9SVWfIqbfw500g Fig 5A ------- COMMENT: 4228fd893e7a1a88 113 VFkskau1qxV6L9SVWfIqbfw500g Fig 5A ------- COMMENT: 4228fd893e7a1a88 114 VFkskau1qxV6L9SVWfIqbfw500g Fig 5A ------- COMMENT: 4228fd893e7a1a88 115 VFkskau1qxV6L9SVWfIqbfw500g Fig 5A ------- COMMENT: 4228fd893e7a1a88 116 VFkskau1qxV6L9SVWfIqbfw500g Fig 5A ------- COMMENT: 4228fd893e7a1a88 117 VFkskau1qxV6L9SVWfIqbfw500g Fig 5A ------- COMMENT: 4228fd893e7a1a88 118 UGKLbMSoFrW9s5nNAMBghTw/TiA Fig 5B ------- COMMENT: 4228fd893e7a1a88 119 UGKLbMSoFrW9s5nNAMBghTw/TiA Fig 5B ------- COMMENT: 4228fd893e7a1a88 120 UGKLbMSoFrW9s5nNAMBghTw/TiA Fig 5B ------- COMMENT: 4228fd893e7a1a88 121 UGKLbMSoFrW9s5nNAMBghTw/TiA Fig 5B ------- COMMENT: 4228fd893e7a1a88 122 UGKLbMSoFrW9s5nNAMBghTw/TiA Fig 5B ------- COMMENT: 4228fd893e7a1a88 123 UGKLbMSoFrW9s5nNAMBghTw/TiA Fig 5B ------- COMMENT: 4228fd893e7a1a88 124 UGKLbMSoFrW9s5nNAMBghTw/TiA Fig 5B ------- COMMENT: 4228fd893e7a1a88 125 UGKLbMSoFrW9s5nNAMBghTw/TiA Fig 5B ------- COMMENT: 4228fd893e7a1a88 126 InAoyMdP/QvHvIvvNV5kmSvzrUU Fig 4B ------- COMMENT: 4228fd893e7a1a88 127 InAoyMdP/QvHvIvvNV5kmSvzrUU Fig 4B ------- COMMENT: 4228fd893e7a1a88 128 InAoyMdP/QvHvIvvNV5kmSvzrUU Fig 4B ------- COMMENT: 4228fd893e7a1a88 129 InAoyMdP/QvHvIvvNV5kmSvzrUU Fig 4B ------- COMMENT: 4228fd893e7a1a88 130 UGKLbMSoFrW9s5nNAMBghTw/TiA Fig 5B ------- COMMENT: 4228fd893e7a1a88 131 UGKLbMSoFrW9s5nNAMBghTw/TiA Fig 5B ------- COMMENT: 4228fd893e7a1a88 132 UGKLbMSoFrW9s5nNAMBghTw/TiA Fig 5B ------- COMMENT: 4228fd893e7a1a88 133 RixlvlgTmMBOZ93/xdU305iyOZI Fig 4A ------- COMMENT: 4228fd893e7a1a88 134 RixlvlgTmMBOZ93/xdU305iyOZI Fig 4A ------- COMMENT: 4228fd893e7a1a88 135 RixlvlgTmMBOZ93/xdU305iyOZI Fig 4A ------- COMMENT: 4228fd893e7a1a88 136 RixlvlgTmMBOZ93/xdU305iyOZI Fig 4A ------- COMMENT: 4228fd893e7a1a88 137 RixlvlgTmMBOZ93/xdU305iyOZI Fig 4A ------- COMMENT: 4228fd893e7a1a88 138 RixlvlgTmMBOZ93/xdU305iyOZI Fig 4A ------- COMMENT: 4228fd893e7a1a88 139 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: 4228fd893e7a1a88 140 wj2VSE893AMBlEWR8+9Ey55om+8 Fig 2B ------- COMMENT: 4228fd893e7a1a88 141 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: 4228fd893e7a1a88 142 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: 4228fd893e7a1a88 143 wj2VSE893AMBlEWR8+9Ey55om+8 Fig 2B ------- COMMENT: 4228fd893e7a1a88 144 wj2VSE893AMBlEWR8+9Ey55om+8 Fig 2B ------- COMMENT: 4228fd893e7a1a88 145 wj2VSE893AMBlEWR8+9Ey55om+8 Fig 2B ------- COMMENT: 4228fd893e7a1a88 146 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: 4228fd893e7a1a88 147 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: 4228fd893e7a1a88 148 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: 4228fd893e7a1a88 149 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: 4228fd893e7a1a88 150 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: 4228fd893e7a1a88 151 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: 4228fd893e7a1a88 152 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: 4228fd893e7a1a88 153 J2w1041UkbciWd6gC5MuYmi9oBA (comment: from polyphosphate absent from cell) ------- COMMENT: 42317f42f3d8dfcb 1 p3x2dDTjQlS9jbO+IMHpHbED4nw (Fig. 1D) ------- COMMENT: 42317f42f3d8dfcb 2 jkt/JJ7T0dHPQWiavwNpv4Gbuo4 (Fig. 1I) ------- COMMENT: 42317f42f3d8dfcb 3 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 42317f42f3d8dfcb 4 NuNHLmtoq4GzF6sDw6D9LwteRCo (Fig. S4B) ------- COMMENT: 42317f42f3d8dfcb 5 bWedWKmvtHbuw91dA24nrSUkQGo (Fig. S5) ------- COMMENT: 42317f42f3d8dfcb 6 uytlu1SOwRPui227BsVN/IEzOy4 (Fig. S2A) ------- COMMENT: 42317f42f3d8dfcb 7 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 42317f42f3d8dfcb 10 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: 42317f42f3d8dfcb 11 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: 42317f42f3d8dfcb 12 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: 42317f42f3d8dfcb 13 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: 42317f42f3d8dfcb 14 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 42317f42f3d8dfcb 15 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 42317f42f3d8dfcb 16 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 42317f42f3d8dfcb 17 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 42317f42f3d8dfcb 18 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 42317f42f3d8dfcb 19 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 42317f42f3d8dfcb 20 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: 42317f42f3d8dfcb 21 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 42317f42f3d8dfcb 22 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 42317f42f3d8dfcb 23 q2ifWLUMPt99XzRAlld7KR3J1LY (Fig. S5C) ------- COMMENT: 42317f42f3d8dfcb 24 1vITJkX365/kSO7rGYqR9cXiOZI (Fig. S4D) ------- COMMENT: 42317f42f3d8dfcb 26 BlZAYgg6W/+/O+S+HMuoElV4g98 (Fig. S6A) ------- COMMENT: 42317f42f3d8dfcb 27 BlZAYgg6W/+/O+S+HMuoElV4g98 (Fig. S6A) ------- COMMENT: 42317f42f3d8dfcb 28 SamTvRZvEvQIrgSzuqc8al8jR1s (Fig. S6B) ------- COMMENT: 42317f42f3d8dfcb 29 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: 42317f42f3d8dfcb 30 BlZAYgg6W/+/O+S+HMuoElV4g98 (Fig. S6A) ------- COMMENT: 42317f42f3d8dfcb 31 uM2FhGtLhiVpB6ojPDDIp5EE4bo (Fig. 5A and 5C) ------- COMMENT: 42317f42f3d8dfcb 33 jTsQneiFN2O87OgZ9cPB1YzCYVU (Fig. S7A) ------- COMMENT: 42317f42f3d8dfcb 34 jTsQneiFN2O87OgZ9cPB1YzCYVU (Fig. S7A) ------- COMMENT: 42317f42f3d8dfcb 35 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: 42317f42f3d8dfcb 36 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: 42317f42f3d8dfcb 37 tJiH8SqrsLc9Ox+diQxNgFAms88 (Fig. S8A) ------- COMMENT: 42317f42f3d8dfcb 38 DVJMM6AvJyLz8J8dkwtY+pFizxc (Fig. 5E) ------- COMMENT: 42317f42f3d8dfcb 40 qgy2UtS49Rc8vkPrVMir2qiYd3M (Fig. 5F) ------- COMMENT: 42317f42f3d8dfcb 41 jTsQneiFN2O87OgZ9cPB1YzCYVU (Fig. S7A) ------- COMMENT: 42317f42f3d8dfcb 42 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: 42317f42f3d8dfcb 43 JT3k9eYP2DeBwb6gtqzco0jbS2M (Fig. 5G) ------- COMMENT: 42317f42f3d8dfcb 44 hewHr1XadQSbYTkmDJqTL0w9LWc (Fig. 5I) ------- COMMENT: 42317f42f3d8dfcb 45 hewHr1XadQSbYTkmDJqTL0w9LWc (Fig. 5I) ------- COMMENT: 42317f42f3d8dfcb 46 U28uRRiodUY3Y1KTMp42IidYEug (Fig. 5H) ------- COMMENT: 42317f42f3d8dfcb 47 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 42317f42f3d8dfcb 48 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 42317f42f3d8dfcb 49 tdY+sTkcVXQXSK7tyg52iDlFg+Y (Fig. S1A) ------- COMMENT: 42317f42f3d8dfcb 50 tdY+sTkcVXQXSK7tyg52iDlFg+Y (Fig. S1A) ------- COMMENT: 42317f42f3d8dfcb 51 xqjTztL6qGqaPgp6WgcG+iOomGI (Fig. S1B) ------- COMMENT: 42317f42f3d8dfcb 52 d2NdxnatvYWNnh9afhcOiSOs0XA (Fig. S1D) ------- COMMENT: 42317f42f3d8dfcb 53 jTsQneiFN2O87OgZ9cPB1YzCYVU (Fig. S7A) ------- COMMENT: 42317f42f3d8dfcb 54 PGCuy5xsv4Ra4c9Evl81+cPA35M (Fig. S9D) ------- COMMENT: 42317f42f3d8dfcb 55 Q+6iRszU2Sgl5Al2OJJ6UDhvr5w (Fig. S8C) ------- COMMENT: 42317f42f3d8dfcb 56 9obkRBahnsvfuKPq+LTlya+asC4 (Fig. 6E) ------- COMMENT: 42317f42f3d8dfcb 57 NLRS8/UNc6jb+3TIzGZPO/pAP3I (Fig. 6C and D) ------- COMMENT: 42317f42f3d8dfcb 58 BJ/rGlQPrl1zT1OiiLiJr6J0KcE (Fig. S8B) ------- COMMENT: 42317f42f3d8dfcb 59 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 42317f42f3d8dfcb 60 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: 42317f42f3d8dfcb 61 HVLL9zNnsbJYh5//n8vC6gJyClM (Fig. S10A) ------- COMMENT: 42317f42f3d8dfcb 62 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: 42317f42f3d8dfcb 63 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 42317f42f3d8dfcb 64 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 42317f42f3d8dfcb 65 p9PqWaxu79GLWhKfMyOCtz3437I (Fig. 7F) ------- COMMENT: 42317f42f3d8dfcb 66 p9PqWaxu79GLWhKfMyOCtz3437I (Fig. 7F) ------- COMMENT: 42317f42f3d8dfcb 67 BgthRc9U4WbpcxmCrg4yU6vEg20 (Fig. S9C) ------- COMMENT: 42317f42f3d8dfcb 68 9Yzox6G/55NB9qHhwbnx+ckLNxQ (Fig. S9A) ------- COMMENT: 42317f42f3d8dfcb 69 m9N4YPI7O+fs/IwNttDzbK8fj5k (Fig. S9B) ------- COMMENT: 42317f42f3d8dfcb 70 RhWCAb96vxLCF2JkKO6eJPai560 (Fig. 1A and Fig. 4A) ------- COMMENT: 42317f42f3d8dfcb 71 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: 42317f42f3d8dfcb 72 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: 42317f42f3d8dfcb 74 biv5vzYpNRqMrXY+5I3wXOO1GLA (Fig. 1G) ------- COMMENT: 42317f42f3d8dfcb 75 xnWwtg3TqqhqbUHXva5kkxV96Hs (Fig. 1H) ------- COMMENT: 42317f42f3d8dfcb 76 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 42317f42f3d8dfcb 77 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 42317f42f3d8dfcb 78 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 42317f42f3d8dfcb 79 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 42317f42f3d8dfcb 80 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 42317f42f3d8dfcb 81 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 42317f42f3d8dfcb 82 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 42317f42f3d8dfcb 83 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: 42317f42f3d8dfcb 84 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: 42317f42f3d8dfcb 85 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: 42317f42f3d8dfcb 86 DVJMM6AvJyLz8J8dkwtY+pFizxc (Fig. 5E) ------- COMMENT: 42317f42f3d8dfcb 87 qgy2UtS49Rc8vkPrVMir2qiYd3M (Fig. 5F) ------- COMMENT: 42317f42f3d8dfcb 88 JT3k9eYP2DeBwb6gtqzco0jbS2M (Fig. 5G) ------- COMMENT: 42317f42f3d8dfcb 89 U28uRRiodUY3Y1KTMp42IidYEug (Fig. 5H) ------- COMMENT: 42317f42f3d8dfcb 90 XwjFKIBpFnAfCL+3uv8ZHt+X4Cc (Fig. 6D) ------- COMMENT: 42317f42f3d8dfcb 91 9obkRBahnsvfuKPq+LTlya+asC4 (Fig. 6E) ------- COMMENT: 42317f42f3d8dfcb 92 /MgdeBHaT9ABix4/xBezuYbm/z8 (Fig. 7E) ------- COMMENT: 42317f42f3d8dfcb 93 /MgdeBHaT9ABix4/xBezuYbm/z8 (Fig. 7E) ------- COMMENT: 42317f42f3d8dfcb 94 n0ymef02YyHEo9XyW9awwNPc6tE (Fig. 6B and Fig. 7G) ------- COMMENT: 42317f42f3d8dfcb 95 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 42317f42f3d8dfcb 96 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 42317f42f3d8dfcb 97 SHphkvIqCxMPqJSlPHh+fBImtrY (Fig. 7G) ------- COMMENT: 42317f42f3d8dfcb 98 d2NdxnatvYWNnh9afhcOiSOs0XA (Fig. S1D) ------- COMMENT: 42317f42f3d8dfcb 99 uytlu1SOwRPui227BsVN/IEzOy4 (Fig. S2A) ------- COMMENT: 42317f42f3d8dfcb 100 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 42317f42f3d8dfcb 101 gA4P48uKuk6ti5ukTRuiFvXo/Zw (Fig. S2C) ------- COMMENT: 42317f42f3d8dfcb 102 NuNHLmtoq4GzF6sDw6D9LwteRCo (Fig. S4B) ------- COMMENT: 42317f42f3d8dfcb 103 bWedWKmvtHbuw91dA24nrSUkQGo (Fig. S5) ------- COMMENT: 42317f42f3d8dfcb 104 SamTvRZvEvQIrgSzuqc8al8jR1s (Fig. S6B) ------- COMMENT: 42317f42f3d8dfcb 105 BgthRc9U4WbpcxmCrg4yU6vEg20 (Fig. S9C) ------- COMMENT: 423c5afa8f3e4209 9 hPFNK9pXn9s6uyKJPTkg3DIM2XE (Fig. 6A) ------- COMMENT: 423c5afa8f3e4209 10 BlZAYgg6W/+/O+S+HMuoElV4g98 (Fig. S6A) ------- COMMENT: 423c5afa8f3e4209 11 hPFNK9pXn9s6uyKJPTkg3DIM2XE (Fig. 6A) ------- COMMENT: 423c5afa8f3e4209 12 hPFNK9pXn9s6uyKJPTkg3DIM2XE (Fig. 6A) ------- COMMENT: 423c5afa8f3e4209 13 hPFNK9pXn9s6uyKJPTkg3DIM2XE (Fig. 6A) ------- COMMENT: 423c5afa8f3e4209 14 hPFNK9pXn9s6uyKJPTkg3DIM2XE (Fig. 6A) ------- COMMENT: 423c5afa8f3e4209 15 hPFNK9pXn9s6uyKJPTkg3DIM2XE (Fig. 6A) ------- COMMENT: 423c5afa8f3e4209 16 hPFNK9pXn9s6uyKJPTkg3DIM2XE (Fig. 6A) ------- COMMENT: 423c5afa8f3e4209 17 hPFNK9pXn9s6uyKJPTkg3DIM2XE (Fig. 6A) ------- COMMENT: 423c5afa8f3e4209 18 hPFNK9pXn9s6uyKJPTkg3DIM2XE (Fig. 6A) ------- COMMENT: 423c5afa8f3e4209 19 hPFNK9pXn9s6uyKJPTkg3DIM2XE (Fig. 6A) ------- COMMENT: 423c5afa8f3e4209 20 BlZAYgg6W/+/O+S+HMuoElV4g98 (Fig. S6A) ------- COMMENT: 423c5afa8f3e4209 21 BlZAYgg6W/+/O+S+HMuoElV4g98 (Fig. S6A) ------- COMMENT: 423c5afa8f3e4209 22 BlZAYgg6W/+/O+S+HMuoElV4g98 (Fig. S6A) ------- COMMENT: 423c5afa8f3e4209 23 BlZAYgg6W/+/O+S+HMuoElV4g98 (Fig. S6A) ------- COMMENT: 423c5afa8f3e4209 24 BlZAYgg6W/+/O+S+HMuoElV4g98 (Fig. S6A) ------- COMMENT: 423c5afa8f3e4209 25 L84RMYxcmJK2Zj7t0R82RKoWDok (Fig. S6C) ------- COMMENT: 423c5afa8f3e4209 26 L84RMYxcmJK2Zj7t0R82RKoWDok (Fig. S6C) ------- COMMENT: 423c5afa8f3e4209 27 L84RMYxcmJK2Zj7t0R82RKoWDok (Fig. S6C) ------- COMMENT: 423c5afa8f3e4209 28 L84RMYxcmJK2Zj7t0R82RKoWDok (Fig. S6C) ------- COMMENT: 423c5afa8f3e4209 29 V1iAQoqm4heXcblKCi7+V8yd0mg (Fig. S6D) ------- COMMENT: 423c5afa8f3e4209 30 V1iAQoqm4heXcblKCi7+V8yd0mg (Fig. S6D) ------- COMMENT: 425e79613088cd53 1 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 425e79613088cd53 2 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 425e79613088cd53 4 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 425e79613088cd53 5 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 425e79613088cd53 6 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 425e79613088cd53 7 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 425e79613088cd53 8 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 428266f53197a2f6 1 a26z7UkPHc++XeLacs55FPjINnE The Meu13-Mcp7 complex activates the initiation step of DNA strand exchange by Dmc1. The Meu13-Mcp7 complex also stimulates Rad51-driven strand exchange to a much less extent in the presence of the Swi5-Sfr1 complex. ------- COMMENT: 428266f53197a2f6 4 RfOu9ymLEXLCSU3GEUfOprpyKnU as the Meu13-Mcp7 complex ------- COMMENT: 428266f53197a2f6 6 RfOu9ymLEXLCSU3GEUfOprpyKnU as the Meu13-Mcp7 complex ------- COMMENT: 428266f53197a2f6 8 RfOu9ymLEXLCSU3GEUfOprpyKnU as the Meu13-Mcp7 complex ------- COMMENT: 428266f53197a2f6 9 RfOu9ymLEXLCSU3GEUfOprpyKnU as the Meu13-Mcp7 complex ------- COMMENT: 428266f53197a2f6 13 a26z7UkPHc++XeLacs55FPjINnE The Meu13-Mcp7 complex activates the initiation step of DNA strand exchange by Dmc1. The Meu13-Mcp7 complex also stimulates Rad51-driven strand exchange to a much less extent in the presence of the Swi5-Sfr1 complex. ------- COMMENT: 428e0a1e7cd80d7a 44 hPFNK9pXn9s6uyKJPTkg3DIM2XE (Fig. 6A) ------- COMMENT: 42ad3cde8bd52174 1 UzZjee1DY7OmrzwE+0gtGxAAeCA Fig. 1, A and B; ------- COMMENT: 42ad3cde8bd52174 2 uYUMljOpH0VDZgtNuOg9LX4lJio Fig. S2, B and C ------- COMMENT: 42ad3cde8bd52174 6 Fr08QTwjTDNZpA5MvkjiwMzxIvc Fig. 3 and Fig. S3, for comparative images of an inserted pro-metaphase wild-type SPB, see Fig. S1 B ------- COMMENT: 42ad3cde8bd52174 8 6PsBd8A32C+BCR8wAss5nZ4oS10 (comment: not required after insertion) ------- COMMENT: 42ad3cde8bd52174 15 UWYyS7dV1JZaYEPAA6UvXnxgeUg Fig. S5 B ------- COMMENT: 42ad3cde8bd52174 18 8G6+j/Mkk6lmbSbCeunxDRKglvY fig 7 ------- COMMENT: 42ad3cde8bd52174 19 UWYyS7dV1JZaYEPAA6UvXnxgeUg Fig. S5 B ------- COMMENT: 42ad3cde8bd52174 20 UWYyS7dV1JZaYEPAA6UvXnxgeUg Fig. S5 B ------- COMMENT: 42ad3cde8bd52174 21 UWYyS7dV1JZaYEPAA6UvXnxgeUg Fig. S5 B ------- COMMENT: 42ad3cde8bd52174 22 UWYyS7dV1JZaYEPAA6UvXnxgeUg Fig. S5 B ------- COMMENT: 42ad3cde8bd52174 23 UWYyS7dV1JZaYEPAA6UvXnxgeUg Fig. S5 B ------- COMMENT: 42ad3cde8bd52174 24 UWYyS7dV1JZaYEPAA6UvXnxgeUg Fig. S5 B ------- COMMENT: 42ad3cde8bd52174 25 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: 42ad3cde8bd52174 26 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: 42ad3cde8bd52174 27 abIPk7JOHa0ElLUGXdBCeyH6ews Fig. 7 (comment: indicated by NDB cholesterol) ------- COMMENT: 42b4f0bd2e01ad3e 2 m0A3uXH0k9iwO5kOXsA5yL/kerE Ectopic Cnp1 preferentially assembles near the nuclear periphery ------- COMMENT: 42b4f0bd2e01ad3e 3 ogUr5VBsnnRqlfbLQlB0iImXZpA Ectopically localized Cnp1 can recruit kinetochore components ------- COMMENT: 42c0feda9c41e26a 47 YkyYJODCXkGsMlLSPa1nL5/8hkk tested using several genes, and reporter construct to test mutations at or near splice sites ------- COMMENT: 42e301a20297a109 47 ZFouCEJwWoNxGijMFC6cgcTlLeg Rng2 subsequently recruits the myosin­II subunits Myo2 and Rlc1. ------- COMMENT: 42f024cd9e96cf6d 10 B+90C4Yq6lT8Z/FuukjncRLBDdo (comment: val: I used this to link to process term even though it isn't shown directly in this paper) ------- COMMENT: 42f024cd9e96cf6d 12 N6VKUTDjCdtPUcAZE1mIKJmo/qw ------- COMMENT: 433d2f2e8d0427b7 4 qNM08F63PXpEa1lbBdGSusQdFj4 (Fig. 4 C) ------- COMMENT: 433d2f2e8d0427b7 5 qNM08F63PXpEa1lbBdGSusQdFj4 (Fig. 4 C) ------- COMMENT: 433d2f2e8d0427b7 6 qNM08F63PXpEa1lbBdGSusQdFj4 (Fig. 4 C) ------- COMMENT: 433d2f2e8d0427b7 7 qNM08F63PXpEa1lbBdGSusQdFj4 (Fig. 4 C) ------- COMMENT: 433d2f2e8d0427b7 8 qNM08F63PXpEa1lbBdGSusQdFj4 (Fig. 4 C) ------- COMMENT: 433d2f2e8d0427b7 9 qNM08F63PXpEa1lbBdGSusQdFj4 (Fig. 4 C) ------- COMMENT: 433d2f2e8d0427b7 10 xbLdy4eUsRrl9dr1+JMIrCO09ew Fig 6 C ------- COMMENT: 433d2f2e8d0427b7 11 xbLdy4eUsRrl9dr1+JMIrCO09ew Fig 6 C ------- COMMENT: 433d2f2e8d0427b7 12 mmm47eIB+v3fDtkw5U8uLX8TaJo Fig S5A C ------- COMMENT: 433d2f2e8d0427b7 13 z6USqnaCVzuHbBZ71iXHEBYtHRg Fig 7 CD (comment: abnormal Q-MT bundle elongation upon G1 re-entry/interphase bundle reassembly) ------- COMMENT: 433d2f2e8d0427b7 14 BFPZcRXHXsB4/chwPJzPrwUt4hw Fig. S5 E ------- COMMENT: 433d2f2e8d0427b7 15 amhq184clge7niSt4aCwL/RxqjE Fig. S5 E ------- COMMENT: 433d2f2e8d0427b7 16 GkHyN2ODQ3cxhpcS7sU7841m2bc Fig 7 E/F ------- COMMENT: 433d2f2e8d0427b7 18 SGGQKldkKIvfwze+3Y3zBe3u+5o Fig 7 E ------- COMMENT: 4364335d0ce165e4 2 PcnK81vbmu+yh2oglmssL11R7/c In the periplasmic space***** glucans were deposited between the PM and the cell wall at the restrictive temperature (Fig 1B). ------- COMMENT: 4364335d0ce165e4 6 gUtCEm9b0JCwYdmb6Rh6C3ka0O0 (comment: assayed using FRAP) ------- COMMENT: 4364335d0ce165e4 12 3wsquR3C8QvD0ydU37xPwzglqzE (comment: using GFP-D4H biosensor) ------- COMMENT: 4364335d0ce165e4 13 DMwFh/q6R4AOpb/dr9hy8BYvujM (comment: Using GFP-D4H biosensor) ------- COMMENT: 4364335d0ce165e4 24 hNSiqq5Oohmfp9If7s8eYym6z6s (ags1-664, bgs1-191 and/or bgs4-1 of α1,3-glucan synthase, linear β1,3-glucan synthase and 1,6 branched β1,3-glucan synthase, respectively) [18,24,39], improved css1-3 cell growth at semi-permissive temperatures (S1B Fig) while growth in hypoosomotic conditions (sorbitol-containing media) did not (S1C Fig). ------- COMMENT: 4364335d0ce165e4 35 KRX6/dz93Mln9dR2NwcTDxu0DL8 we predicted that the glucan synthases Ags1, Bgs1, Bgs3, and Bgs4 would localize normally and adjacent to the glucan deposits in css1-3 mutant cells. Indeed, all four proteins localized at tips and septa of css1-3, as in wildtype cells ------- COMMENT: 4364335d0ce165e4 43 NvVMZegcpK/ntIpgA/BmCMzFkSg Indeed, we isolated a cold-sensitive mutant of cut6, cut6-1, as a strong suppressor of css1-3 (S3A Fig). ------- COMMENT: 43659e6aa472ef94 1 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 43659e6aa472ef94 2 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 43659e6aa472ef94 3 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 43659e6aa472ef94 4 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 43659e6aa472ef94 5 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 43659e6aa472ef94 6 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 43659e6aa472ef94 7 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 43659e6aa472ef94 8 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 43659e6aa472ef94 9 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 43659e6aa472ef94 10 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 43659e6aa472ef94 11 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 43659e6aa472ef94 12 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 43659e6aa472ef94 13 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: 43659e6aa472ef94 19 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: 43659e6aa472ef94 20 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: 43659e6aa472ef94 21 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: 43659e6aa472ef94 22 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: 43659e6aa472ef94 23 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: 43659e6aa472ef94 24 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 43659e6aa472ef94 25 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 43659e6aa472ef94 26 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 43659e6aa472ef94 27 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 43659e6aa472ef94 28 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 43659e6aa472ef94 29 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 43659e6aa472ef94 30 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 43659e6aa472ef94 31 vkT8uMpiBxgGlmYXEA8V3+LXigM Fig. 4E ------- COMMENT: 43659e6aa472ef94 32 ozef4U+EHPBfEZa6UOvkx4N9txA We report crystal structures of Pce1 bound to Pol2 CTD and Spt5 CTD ligands. Key findings are that (1) the CTDs of Pol2 and Spt5 interact with completely distinct sites on the NTase and OB domains of the fission yeast GTase, respectively, and (2) whereas the interface of GTase with the Pol2 CTD is dependent on Ser5 phosphorylation, GTase binding to Spt5 CTD is antagonized by Thr1 phosphor- ylation. ------- COMMENT: 43659e6aa472ef94 33 ozef4U+EHPBfEZa6UOvkx4N9txA We report crystal structures of Pce1 bound to Pol2 CTD and Spt5 CTD ligands. Key findings are that (1) the CTDs of Pol2 and Spt5 interact with completely distinct sites on the NTase and OB domains of the fission yeast GTase, respectively, and (2) whereas the interface of GTase with the Pol2 CTD is dependent on Ser5 phosphorylation, GTase binding to Spt5 CTD is antagonized by Thr1 phosphor- ylation. ------- COMMENT: 439dbaa9865b09a8 1 2iemwVtKSl95pOxo+PvDKEQaH84 Fig1A,B In the presence of MBC the % cut cells in wild type is almost identical to mad2 Delta at 32 and 35°c suggesting that the spindle assembly checkpoint is not active or overidden above 32°C in wild type cells in presence of MBC. ------- COMMENT: 439dbaa9865b09a8 2 b/W/YC3L0U5kOg0yP2N2gsIXCDA Fig1C ------- COMMENT: 439dbaa9865b09a8 4 yquSVQOPpp3pXIjyxWokeAS7PQo Fig1E ------- COMMENT: 439dbaa9865b09a8 5 3b3QSoBCKuA+mYiq+JcZzX6++1w Fig1D ------- COMMENT: 439dbaa9865b09a8 6 3b3QSoBCKuA+mYiq+JcZzX6++1w Fig1D ------- COMMENT: 439dbaa9865b09a8 7 3b3QSoBCKuA+mYiq+JcZzX6++1w Fig1D ------- COMMENT: 439dbaa9865b09a8 8 XC6/Xyd9y+veWK7Sgo8YR3NgLTc Fig1D; microtubules absent ------- COMMENT: 439dbaa9865b09a8 9 EwLpwneU6ju5ywO2L81T+nca5a4 Fig2 A ------- COMMENT: 439dbaa9865b09a8 10 B+zTHR2KblR6gYbgAk8Dr8e7BsU Fig2B in this case (high temp no MBC) the checkpoint is active at high temperature and cells are blocked in cell cycle progression at 36°C. ------- COMMENT: 439dbaa9865b09a8 11 EvqXCE0yUiFvXT5CyePquxC5kjY Fig2c ------- COMMENT: 439dbaa9865b09a8 12 Vve3eI2Zdqyit/1wdKdYz/eWwxs Fig2c in presence of MBC cells re-enter S phase earlier than in the absence of MBC ------- COMMENT: 439dbaa9865b09a8 13 QpXhxy5gVVWl5SCQbuhTQ4BTXnE Fig3B just a short microtubule stub remains ------- COMMENT: 439dbaa9865b09a8 14 hKWCsgQ9BP4CeUjS4JB+UtD3J0c Fig3C ------- COMMENT: 439dbaa9865b09a8 16 D53M4dB7os0sS6CF6SQVTnhYn3o Fig3 D ------- COMMENT: 439dbaa9865b09a8 17 qhx3HG0+GcL5i1eu1CCDxX1TIcw Fig 3F ------- COMMENT: 439dbaa9865b09a8 18 7h37NKGLaAGySrS9Cg5wDudL97w Fig3E ------- COMMENT: 439dbaa9865b09a8 19 BCfCFHa5L2g/vfdy/ZIPS7Lld24 Video S3 ------- COMMENT: 439dbaa9865b09a8 21 MqAEDyVSkrj5qnyQ7z52NIX75K0 Fig 3G (comment: suggests nuclear fission is independent of spindle checkpoint) ------- COMMENT: 439dbaa9865b09a8 23 PXqQ8w7WZKoenOmS6L1/H8ouUb4 Fig 4A ------- COMMENT: 439dbaa9865b09a8 24 Ozp47kvNh5k2BQB6BeY/MCsDsaY Fig 4D ------- COMMENT: 439dbaa9865b09a8 25 O5ChQshWo9SMl9ef8VwaxdF3zLk Fig 4B ------- COMMENT: 439dbaa9865b09a8 26 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 439dbaa9865b09a8 27 e9zLO4myWERWdNPG0V7qsXIluWQ Fig 4C (comment: followed the presence of clp1 in the nucleolus to monitor cen3) ------- COMMENT: 439dbaa9865b09a8 28 2tx/Xst/KthHOH0haHeCtGWJWZk Fig 4E ------- COMMENT: 439dbaa9865b09a8 29 s8zxygB4j9+cKrsniSSlYhY+9P0 Fig 5A ------- COMMENT: 439dbaa9865b09a8 31 pXsBPUBMO0Tb41orIiJk4WI2ThU Fig 2B in this case (high temp + MBC) cells can proceed through cell cycle and replicate their DNA ------- COMMENT: 439dbaa9865b09a8 34 JAi5RwxkYYqQDd3vswCFtD8KbUA Fig6D,E in presence of LatA + MBC there is no SPB separation compared to + MBC only where SPBs can separate ------- COMMENT: 43e8214a9219b277 1 xRCxjUTwxuLagQ1XdqAqk1Hg9gc ------- COMMENT: 43e8214a9219b277 2 g+F6FaR+vQ1a6H3WMpYiHGdmy90 We then examined whether cytosolic ribosomes still tethered to mitochondria in Δdnm1 cells after prolonged glucose depletion. In situ cryo-ET imaging of Δdnm1 cells revealed that mito- chondria remained elongated throughout glucose depletion (Fig. 4b). However, this morphological change did not prevent the tethering of cytosolic ribosomes to the OMM, with 98% of the imaged mitochon- dria (n = 100) being fully decorated with ribosomes at day 7 of glucose depletion (Fig. 4b, c, Supplementary Fig. 10 and Supplementary Movie 2). ------- COMMENT: 43e8214a9219b277 3 JzwAKMjncTp+jNFYR5xig+K5Cs4 While Δcpc2 cells did not display discernible growth defects under nutrient-rich conditions, significant growth impairments were evident when these cells were cultivated in defined EMM media supplemented with either 2% or 0.5% glucose concentrations. ------- COMMENT: 43e8214a9219b277 4 RBn8SbHUqi/yB7/ALQ+jv4+lz/U We then used in situ cryo-ET to assess whether ribo- somal tethering to mitochondria was affected in the Δcpc2 strain. The tomograms showed fragmented circular mitochondria, as observed in WT cells, while no ribosome tethering was observed after 7 days of cells growing at low glucose concentrations (Fig. 5c, d, Supplementary Fig. 10 and Supplementary Movie 3). ------- COMMENT: 43e8214a9219b277 6 xRCxjUTwxuLagQ1XdqAqk1Hg9gc ------- COMMENT: 43e8214a9219b277 7 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 8 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 9 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 10 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 11 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 12 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 13 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 14 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 15 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 16 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 17 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 18 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 19 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 20 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 21 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 22 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 23 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 24 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 25 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 26 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 27 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 28 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 29 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 30 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 31 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 32 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 33 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 34 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 35 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 36 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 37 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 38 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 39 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 40 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 41 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 42 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 43 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 44 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 45 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 46 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 47 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 48 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 49 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 50 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 51 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 52 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 53 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 54 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 55 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 56 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 57 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 58 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 59 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 60 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 61 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 62 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 63 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 64 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 65 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 66 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 67 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 68 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 69 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 70 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 71 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 72 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 73 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 74 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 75 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 76 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 77 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43e8214a9219b277 78 MxzL1UbWpoW0i4d3xMPf19fXeZU ------- COMMENT: 43ecf1cd34feb2eb 1 nApuGrSjVQ/Ns+8CBjiSFc+QxkU Myo51 were indistinguishable from an isogenic wild-type strain (Fig. 2A,B). ------- COMMENT: 43ecf1cd34feb2eb 2 Eqtb9KZYA0jrsF2fJMHinIe8YIw At this temperature myo52∆ cells grew more slowly (Fig. 2B) and had an altered morphology, being shorter and ------- COMMENT: 43ecf1cd34feb2eb 3 Eqtb9KZYA0jrsF2fJMHinIe8YIw At this temperature myo52∆ cells grew more slowly (Fig. 2B) and had an altered morphology, being shorter and ------- COMMENT: 43ecf1cd34feb2eb 4 yHO1kPXOiWaqqiMhcFj+h1wp45Q septation index of 20%, twice that of wild type (Fig. 2A). ------- COMMENT: 43ecf1cd34feb2eb 5 BrZfnL+EqgNm/Ah5SJWIavSzB7s myo52∆ cells showed extremely slow growth at 36°C (Fig. 2C). ------- COMMENT: 43ecf1cd34feb2eb 6 lyDWMC4+RHvbPeKytgyB+oE8NaU Loss of cell shape in myo52∆ was accompanied by the depolarisation of the actin cytoskeleton whereas no change in actin organisation could be detected in myo51∆ (Fig. 3A). ------- COMMENT: 43ecf1cd34feb2eb 7 Nk6kbj5lcgkOmzpV8IFrpHiwEAA (Fig. 3B). The double mutant myo52∆ cps8− was extremely sick even at the permissive temperature and formed only micro colonies consisting of severely deformed cells. ------- COMMENT: 43ecf1cd34feb2eb 8 3Nw7uR/w4jOgJ/DRuV2KTC6hIww (comment: figure missing from pdf, so I could not be more precise myo51∆ cps8− cells were viable at 25°C but had marked cytokinetic defects) (Fig. 3B). ------- COMMENT: 43ecf1cd34feb2eb 9 tz/Bh/T3hU46yPxsMdebqk/RHIw Whereas Myo51 overproduction resulted in elongated cells with wispy, mis- oriented septal material (Fig. 4A) ------- COMMENT: 43ecf1cd34feb2eb 10 cADjESyzcpOwxAKGr6u1y4+hjro cells overproducing Myo52 were less elongated and branched due to the failure of septa to properly cleave (Fig. 4B). ------- COMMENT: 43ecf1cd34feb2eb 11 EotaO1q76qku9yJ/TZmR+GTUT4s Myo52 formed a cap at the growing tips (Fig. 5C,D). ------- COMMENT: 43ecf1cd34feb2eb 12 EotaO1q76qku9yJ/TZmR+GTUT4s Myo52 formed a cap at the growing tips (Fig. 5C,D). ------- COMMENT: 43ecf1cd34feb2eb 13 aRo1St7NqOd9+/i3aGIn0GHWRCs Myo51 appears to be a component of the CAR. ------- COMMENT: 43ecf1cd34feb2eb 14 FHQnOvux/8KDG08H81hxLch4c7A Mok1 was delocalised in myo52∆ (Fig. 8C) ------- COMMENT: 43f8a34aa26e2202 1 PYmumnJCx/3EP34OFZKHqP+ruPs Fig 2. (comment: CHECK Cdc2-DL2 over expressed from an integrated pREP1 (pMNS21) plasmid.) ------- COMMENT: 43f8a34aa26e2202 2 OgqUHG6TP5tC0U6gJWl2hl9dBYI Fig 4 (comment: CHECK Histone H1 used as substrate. Cdc2-DL2 over expressed from an integrated pREP1 (pMNS21) plasmid.) ------- COMMENT: 43f8a34aa26e2202 3 dtCCRZD0BRVuOBfjLZlARdIueag Fig 5. (comment: Cdc2-DL2 over expressed from an integrated pREP1 (pMNS21) plasmid. Phosphorylation on threonine, but position(s) not determined.) ------- COMMENT: 43f8a34aa26e2202 5 xr+qZz8YntjDclJb4d37p825IcQ (comment: CHECK cdc13 expressed from own promoter on multi copy plasmid pUR18) ------- COMMENT: 43f8a34aa26e2202 6 Aj3dhpWcoIt+7F/DX+KG3Gd2H80 Fig 3. (comment: CHECK Cdc2-DL2 over expressed from an integrated pREP1 (pMNS21) plasmid.) ------- COMMENT: 43f8a34aa26e2202 7 6Va/g+lo1dXz5sTJoU62Da88e9Q Fig 7 (comment: HECK Cdc2-DL2 over expressed from an integrated pREP1/pMNS21 plasmid). (comment: Binds to cdc13 but this is reduced compared to binding of cdc2+ to cdc13) ------- COMMENT: 43f9f82f0a5c5c77 1 TvJ2KKzZJ35+yEqLG9mG3u/AKLM (comment: CHECK The defect was observed in rec12-deletion rec8-2A mutant background) Fig 3C. ------- COMMENT: 43f9f82f0a5c5c77 2 Aw4M8g2Uq7sznjsxPhy7T7vzy/g (comment: CHECK The defect was observed in the rec12-deletion rec8-2A mutation background. The defect of fta2-9D was less than that of fta2-9A) Fig 3C. ------- COMMENT: 43f9f82f0a5c5c77 3 GDFDIHl7bGD46w4ShSW4WvMBzUA Fig. 2 (comment: CHeCK TBZ sensitivity testing for wild-type, 9A, and 9D) ------- COMMENT: 43f9f82f0a5c5c77 4 GDFDIHl7bGD46w4ShSW4WvMBzUA Fig. 2 (comment: CHECK TBZ sensitivity testing for wild-type, 9A, and 9D) ------- COMMENT: 44220f03945c034e 5 MI/tmcoxX4bxaCwhNbO+aM9eXUM (comment: penetrance at 4 hours; increases upon longer time at restrictive temp) ------- COMMENT: 44220f03945c034e 10 EVs6Ze/ZCVMRucexsQT1+Q30USs (comment: same with or without TBZ) ------- COMMENT: 44220f03945c034e 11 EVs6Ze/ZCVMRucexsQT1+Q30USs (comment: same with or without TBZ) ------- COMMENT: 44220f03945c034e 32 e+G2/AwtUvH0faHJ/FCPkNFapsQ (comment: penetrance at 4 hours) ------- COMMENT: 44220f03945c034e 35 e+G2/AwtUvH0faHJ/FCPkNFapsQ (comment: penetrance at 4 hours) ------- COMMENT: 4436cb3ee9a644ac 17 GvzrGMCgnRBdXyr0B/dXWprCBN0 (comment: probably Y173, but not determined experimentally) ------- COMMENT: 4436cb3ee9a644ac 33 GvzrGMCgnRBdXyr0B/dXWprCBN0 (comment: probably Y173, but not determined experimentally) ------- COMMENT: 4436cb3ee9a644ac 42 GvzrGMCgnRBdXyr0B/dXWprCBN0 (comment: probably Y173, but not determined experimentally) ------- COMMENT: 443d8e38e12610f6 1 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 443d8e38e12610f6 2 8G25CDQom9QgXEKi+SR7jFK3rvE Fig. 1D, Fig. 2 ------- COMMENT: 443d8e38e12610f6 3 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 443d8e38e12610f6 4 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 443d8e38e12610f6 5 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 443d8e38e12610f6 6 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 443d8e38e12610f6 7 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 443d8e38e12610f6 8 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 443d8e38e12610f6 9 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 443d8e38e12610f6 10 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 443d8e38e12610f6 11 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 443d8e38e12610f6 12 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 443d8e38e12610f6 13 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: 443d8e38e12610f6 14 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 443d8e38e12610f6 15 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 443d8e38e12610f6 16 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 443d8e38e12610f6 21 b/fcStrfD1m4iFiVYMFtPDcufco Fig 1,3, & 4 ------- COMMENT: 443d8e38e12610f6 22 7Ub1+CI9H9KZmMOQfFysIkJm17c Fig. 1 C ------- COMMENT: 44791e9e5116fe71 1 CoRj1P1GzBhI6hV7phVZO0C47dQ Second, Any1R175C does not show an increase but rather a strong decrease in its ubiquiti- nation level. ------- COMMENT: 44791e9e5116fe71 3 W0iC/SZ1AVLPjkHlUIZ5Hs/kzcU canavanine resistance in cat1-d slightly enhanced in vhc1-d ------- COMMENT: 44791e9e5116fe71 4 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 44791e9e5116fe71 6 ZqNsguoL+zP6H3eqfbuL4xctrSE (comment: CHECK Increased protein ubiquitination.) ------- COMMENT: 44791e9e5116fe71 8 AVbx7pOndTw+O9ruDb52p7r/HUQ Figure 1 ------- COMMENT: 44791e9e5116fe71 9 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 44791e9e5116fe71 11 FSHCBikOxUBpCPgNFkcVKyLdVTc Figure 1 (comment: same as WT) ------- COMMENT: 44791e9e5116fe71 12 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 44791e9e5116fe71 13 FJ+V3yp56jjQvlswbOfJB1wcS+s Figure 3 (comment: confirms dominance of can1-1) ------- COMMENT: 4488533ca5f8fdc3 4 LWB+cHH3vkWYEpS+/iOF7sEw72M (comment: Tev protease present; Cdc23 truncated) ------- COMMENT: 4488533ca5f8fdc3 6 LWB+cHH3vkWYEpS+/iOF7sEw72M (comment: Tev protease present; Cdc23 truncated) ------- COMMENT: 4488533ca5f8fdc3 8 d3rp6k1Z4CgUo/+MmHpVQGAHtBI (comment: Tev protease present; Cdc23 truncated; N starvation/recovery synchronizes cells) ------- COMMENT: 4488533ca5f8fdc3 10 SZsjWaj40sBOdl7MIhS4QhG6Irk (comment: Tev protease present; Cdc23 truncated; cells not synchronized) ------- COMMENT: 4488533ca5f8fdc3 12 FntFm+5SvGuOJN+nndXfl/d6y2g (comment: Tev protease present; Cdc23 truncated; Cdc23 C-terminal fragment not retained in nucleus) ------- COMMENT: 4488533ca5f8fdc3 14 FntFm+5SvGuOJN+nndXfl/d6y2g (comment: Tev protease present; Cdc23 truncated; Cdc23 C-terminal fragment not retained in nucleus) ------- COMMENT: 44ae5ece389a1b0a 1 p/MhGTyuMTC/USmwnEct49pEHKU (comment: TEV protease present; Cdc6 truncated) ------- COMMENT: 44ae5ece389a1b0a 17 p/MhGTyuMTC/USmwnEct49pEHKU (comment: TEV protease present; Cdc6 truncated) ------- COMMENT: 44e84d2e1bfe62fa 1 CTswP1FWoTwX2pYZfvFuf+9/mtQ The two exceptions were the cox10 and cox15 mutants, both of which lacked heme A. In agreement with earlier results the cox10 mutant also had no heme O [4]. This was not true of the cox15 mutant which had a very low but detectable amount of heme O (Fig. 1). ------- COMMENT: 44e84d2e1bfe62fa 2 WYFOqHnSwdGo5eCGDtOVhertgGU The two exceptions were the cox10 and cox15 mutants, both of which lacked heme A. In agreement with earlier results the cox10 mutant also had no heme O [4]. ------- COMMENT: 44e84d2e1bfe62fa 3 THMqvPezrWE5xXlYAbF0VJCxhc4 We propose that the two proteins are part of a mitochondrial (prokaryotic) type monooxygenase that hydroxylates the methyl group of heme O. Accordingly, the third component of this pathway is ferredoxin (adrenodoxin) dehydrogenase encoded by ARH1 which has been localized in mitochondria of S. cerevisiae [13]. As depicted in Fig. 3, the function of Cox15p would be analogous to that of P450 in other three component monooxygenases [14]. ------- COMMENT: 44e84d2e1bfe62fa 7 U/TZYbk8eFwhhS1Oi01Qhvl/IWY These results further indicate that the C-terminal end of Cox15p, together with Yah1p, is located in the matrix com- partment. Yah1p fused to Cox15p is therefore in the same compartment as the native protein (Fig. 5B). ------- COMMENT: 44e84d2e1bfe62fa 8 bJuPj9WcP2MULcMQ4Dqur2XHYyg This was not true of the cox15 mutant which had a very low but detectable amount of heme O (Fig. 1). ------- COMMENT: 44e84d2e1bfe62fa 9 P1X9Q8MHAdmaeS1IK53j9JToTyk In agreement with earlier results the cox10 mutant also had no heme O [4]. This was not true of the cox15 mutant which had a very low but detectable amount of heme O (Fig. 1). ------- COMMENT: 45245ca07b498e71 1 45Rz0pIWyxnVAmO+y5l69KC2YNU we did not detect Csc1-R31A-GFP on SPBs at any point in the cell cycle (Figure 1C). ------- COMMENT: 45245ca07b498e71 2 J52AJiafANG5KsoAtbd/YrhsYCE Thus, we tested whether csc1-R31A interacted negatively with cdc16-116 cells and found that it did (Figure 2C). ------- COMMENT: 45245ca07b498e71 3 CNWbbzVz2oL4nFZkE1hCK7nqQBQ SIP null mutants rescue cdc11- 136 (Singh et al., 2011) and we observed that csc1-R31A also did (Figure 2D). ------- COMMENT: 45245ca07b498e71 5 Mq8vxUJWlmLCODQQDVm4w3RMi6c Like ppa3∆, ppa3-D82N cells were viable and 9 rescued growth of cdc11-136, an indication that it is a loss-of-function ppa3 allele (Figure S3A) ------- COMMENT: 45245ca07b498e71 6 AlUQ7euRShYW1FbZfmBG1hGty+M We found that this is also true for Csc4. Csc4-GFP was not detected at SPBs in csc1∆, csc2∆, csc3∆ or ppa3∆ cells (Figure S1A). ------- COMMENT: 45245ca07b498e71 7 AlUQ7euRShYW1FbZfmBG1hGty+M We found that this is also true for Csc4. Csc4-GFP was not detected at SPBs in csc1∆, csc2∆, csc3∆ or ppa3∆ cells (Figure S1A). ------- COMMENT: 45245ca07b498e71 8 AlUQ7euRShYW1FbZfmBG1hGty+M We found that this is also true for Csc4. Csc4-GFP was not detected at SPBs in csc1∆, csc2∆, csc3∆ or ppa3∆ cells (Figure S1A). ------- COMMENT: 45245ca07b498e71 10 90AfHbLZfBFPFHxaNW4dLVHS0Ss We also did not detect Csc2-GFP, Csc3-GFP or Csc4-GFP at SPBs in csc1-R31A cells although these proteins were detected at SPBs in wildtype cells (Figure S1B). ------- COMMENT: 45245ca07b498e71 11 90AfHbLZfBFPFHxaNW4dLVHS0Ss We also did not detect Csc2-GFP, Csc3-GFP or Csc4-GFP at SPBs in csc1-R31A cells although these proteins were detected at SPBs in wildtype cells (Figure S1B). ------- COMMENT: 45245ca07b498e71 12 90AfHbLZfBFPFHxaNW4dLVHS0Ss We also did not detect Csc2-GFP, Csc3-GFP or Csc4-GFP at SPBs in csc1-R31A cells although these proteins were detected at SPBs in wildtype cells (Figure S1B). ------- COMMENT: 45245ca07b498e71 13 EgfBIWv21yXiFvkhyuzwPwtQbOg we found that Cdc7 and Sid1 localized symmetrically to both SPBs during anaphase in contrast to its asymmetrical distribution to a single SPB in wildtype anaphase cells (Figure 2, A and B). ------- COMMENT: 45245ca07b498e71 14 EgfBIWv21yXiFvkhyuzwPwtQbOg we found that Cdc7 and Sid1 localized symmetrically to both SPBs during anaphase in contrast to its asymmetrical distribution to a single SPB in wildtype anaphase cells (Figure 2, A and B). ------- COMMENT: 45245ca07b498e71 15 GDnQkD/aUcbY3W/edmTRnDhoY9g In both wildtype and ppa3-D82N cells, Csc1-GFP localized to mitotic SPBs (Figure 4C). ------- COMMENT: 45245ca07b498e71 16 McGvsJLiTeEeAt5l7o312dxJZW4 ppa3-D82N cells producing Sid4-RFP displayed symmetric Sid1-GFP and Cdc7-GFP localization on anaphase SPBs whereas the localization is asymmetric in wildtype cells (Figure 4B). This further demonstrates that ppa3-D82N is a loss of function allele. ------- COMMENT: 45245ca07b498e71 17 McGvsJLiTeEeAt5l7o312dxJZW4 ppa3-D82N cells producing Sid4-RFP displayed symmetric Sid1-GFP and Cdc7-GFP localization on anaphase SPBs whereas the localization is asymmetric in wildtype cells (Figure 4B). This further demonstrates that ppa3-D82N is a loss of function allele. ------- COMMENT: 45245ca07b498e71 24 MkKPM6OULGz6kLUJovYjBupAidM GFP-Csc1(FHA) localized to SPBs in mitotic cells (Figure 1D). ------- COMMENT: 45245ca07b498e71 25 IlTAJRjHBJGi+mbWU1WVXu5M6jE is independent of the SIN because GFP- Csc1(FHA) expressed from the nmt81 promoter of pREP81 localized to mitotic SPBs marked by Sad1-mCherry in the SIN scaffold mutant sid4-SA1 cells at the restrictive temperature (Figure 1E). ------- COMMENT: 45245ca07b498e71 26 ygfDmEDVE01q0Euo5kcudT81P/w Interestingly, we found that cells producing both Csc1-GFP and either Ppc89-GBP-mCherry (Figure S2A) or Sid4-GBP-mCherry (Figure 3A) were inviable. ------- COMMENT: 45245ca07b498e71 27 u7whOb+REdwHYIu7xIx/K/Ja104 We found that cells producing both Csc1-R31A-GFP and Ppc89-GBP-mCherry (Figure S2B) or Sid4-GBP-mCherry (Figure 3B) were also inviable. ------- COMMENT: 45245ca07b498e71 28 ygfDmEDVE01q0Euo5kcudT81P/w Interestingly, we found that cells producing both Csc1-GFP and either Ppc89-GBP-mCherry (Figure S2A) or Sid4-GBP-mCherry (Figure 3A) were inviable. ------- COMMENT: 45245ca07b498e71 29 /RlsTiUFrbOmvCgrApru1YBJBSE died as single elongated cells, consistent with SIN inactivation (Figure S2, C and D) ------- COMMENT: 45245ca07b498e71 30 /RlsTiUFrbOmvCgrApru1YBJBSE died as single elongated cells, consistent with SIN inactivation (Figure S2, C and D) ------- COMMENT: 45245ca07b498e71 31 /RlsTiUFrbOmvCgrApru1YBJBSE died as single elongated cells, consistent with SIN inactivation (Figure S2, C and D) ------- COMMENT: 45245ca07b498e71 32 Mq8vxUJWlmLCODQQDVm4w3RMi6c Like ppa3∆, ppa3-D82N cells were viable and 9 rescued growth of cdc11-136, an indication that it is a loss-of-function ppa3 allele (Figure S3A) ------- COMMENT: 45395a087bd927e5 13 zSKTbWOeMX3CdZBKogDEzB19Jog figure S1 ------- COMMENT: 45395a087bd927e5 14 zSKTbWOeMX3CdZBKogDEzB19Jog figure S1 ------- COMMENT: 45395a087bd927e5 15 zSKTbWOeMX3CdZBKogDEzB19Jog figure S1 ------- COMMENT: 45395a087bd927e5 16 zSKTbWOeMX3CdZBKogDEzB19Jog figure S1 ------- COMMENT: 45395a087bd927e5 17 zSKTbWOeMX3CdZBKogDEzB19Jog figure S1 ------- COMMENT: 45395a087bd927e5 18 zSKTbWOeMX3CdZBKogDEzB19Jog figure S1 ------- COMMENT: 45395a087bd927e5 19 zSKTbWOeMX3CdZBKogDEzB19Jog figure S1 ------- COMMENT: 45395a087bd927e5 20 zSKTbWOeMX3CdZBKogDEzB19Jog figure S1 ------- COMMENT: 45395a087bd927e5 21 zSKTbWOeMX3CdZBKogDEzB19Jog figure S1 ------- COMMENT: 45395a087bd927e5 25 q2mAB6kt8C4o0UmaEjHe6uDvy8U (comment: CHECK abnormal RNA localization to chromatin) ------- COMMENT: 45395a087bd927e5 31 RPdkqAedukJ0cWGJswoHwJ+rPAE figure 3 ------- COMMENT: 45395a087bd927e5 32 RPdkqAedukJ0cWGJswoHwJ+rPAE figure 3 ------- COMMENT: 45395a087bd927e5 33 +0MVC3YLwqzqdGSgSzbreMRgGc4 (comment: the def includes "maintenance of lcoalization WITHIN nucleus" so it fits the def, but maybe the term looks weird) ------- COMMENT: 45395a087bd927e5 46 jJwxmhVPGMY9AM3Z094vKDsuv1o (comment: expression of meiosis specific transcripts increased during vegetative growth at lower temps) ------- COMMENT: 45395a087bd927e5 47 f/3+vVZlUPhO2rTw8k2fDPiT48o (comment: at sme2 locus -one of several exosome foci in nucleus during vegetative growth) ------- COMMENT: 459a6e9d469b1af5 2 6l7Mkb6PXnLDXHrSewn7m01mnsg (comment: cells otherwise haploid) ------- COMMENT: 459a6e9d469b1af5 3 6l7Mkb6PXnLDXHrSewn7m01mnsg (comment: cells otherwise haploid) ------- COMMENT: 459a6e9d469b1af5 4 6l7Mkb6PXnLDXHrSewn7m01mnsg (comment: cells otherwise haploid) ------- COMMENT: 459a6e9d469b1af5 6 EHG4nZ6lZZfhNlkSUuEJu+asA7E (comment: CHECK residue T11) ------- COMMENT: 459a6e9d469b1af5 7 EHG4nZ6lZZfhNlkSUuEJu+asA7E (comment: CHECK residue T11) ------- COMMENT: 459a6e9d469b1af5 8 EHG4nZ6lZZfhNlkSUuEJu+asA7E (comment: CHECK residue T11) ------- COMMENT: 459a6e9d469b1af5 9 EHG4nZ6lZZfhNlkSUuEJu+asA7E (comment: CHECK residue T11) ------- COMMENT: 459a6e9d469b1af5 10 EHG4nZ6lZZfhNlkSUuEJu+asA7E (comment: CHECK residue T11) ------- COMMENT: 459a6e9d469b1af5 11 EHG4nZ6lZZfhNlkSUuEJu+asA7E (comment: CHECK residue T11) ------- COMMENT: 459a6e9d469b1af5 13 OKD5YNd0slZGLUKzvBfH2SoxpU4 (comment: CHECK assayed substrate myelin basic protein) ------- COMMENT: 459a6e9d469b1af5 14 k6w19bcCilG37d9l/Ep6tkjLSKU (comment: CHECK effect of mutation in substrate Cds1 molecule) ------- COMMENT: 459a6e9d469b1af5 15 OKD5YNd0slZGLUKzvBfH2SoxpU4 (comment: CHECK assayed substrate myelin basic protein) ------- COMMENT: 459a6e9d469b1af5 16 OKD5YNd0slZGLUKzvBfH2SoxpU4 (comment: CHECK assayed substrate myelin basic protein) ------- COMMENT: 459a6e9d469b1af5 17 OKD5YNd0slZGLUKzvBfH2SoxpU4 (comment: CHECK assayed substrate myelin basic protein) ------- COMMENT: 459a6e9d469b1af5 18 OKD5YNd0slZGLUKzvBfH2SoxpU4 (comment: CHECK assayed substrate myelin basic protein) ------- COMMENT: 459a6e9d469b1af5 19 OKD5YNd0slZGLUKzvBfH2SoxpU4 (comment: CHECK assayed substrate myelin basic protein) ------- COMMENT: 459a6e9d469b1af5 20 OKD5YNd0slZGLUKzvBfH2SoxpU4 (comment: CHECK assayed substrate myelin basic protein) ------- COMMENT: 459a6e9d469b1af5 21 k6w19bcCilG37d9l/Ep6tkjLSKU (comment: CHECK effect of mutation in substrate Cds1 molecule) ------- COMMENT: 459a6e9d469b1af5 22 k6w19bcCilG37d9l/Ep6tkjLSKU (comment: CHECK effect of mutation in substrate Cds1 molecule) ------- COMMENT: 459a6e9d469b1af5 23 9x11JomiiuIUCkVS0kh6iLAAgeU induced dimerization increases Cds1 autophosphorylation without prior phosphorylation on T11 ------- COMMENT: 459a6e9d469b1af5 24 TnaLatJlflk+FfPyBMc7OQkjVIo abolished dimerization in kinase-dead cds1-D312E ------- COMMENT: 459a6e9d469b1af5 25 OKD5YNd0slZGLUKzvBfH2SoxpU4 (comment: CHECK assayed substrate myelin basic protein) ------- COMMENT: 459a6e9d469b1af5 28 OKD5YNd0slZGLUKzvBfH2SoxpU4 (comment: CHECK assayed substrate myelin basic protein) ------- COMMENT: 459a6e9d469b1af5 29 OKD5YNd0slZGLUKzvBfH2SoxpU4 (comment: CHECK assayed substrate myelin basic protein) ------- COMMENT: 459a6e9d469b1af5 30 OKD5YNd0slZGLUKzvBfH2SoxpU4 (comment: CHECK assayed substrate myelin basic protein) ------- COMMENT: 459a6e9d469b1af5 33 HlO+1UUkzP+JM6FOCrPK53QJz+k (comment: CHECK has output PR:000037300) ------- COMMENT: 45b6d1dcc31ecbde 5 NkVMmaP8TD0t1EcgkLVpD3Ja0lY (comment: CHECK proteins dissociate during cellular response to UV) ------- COMMENT: 45b6d1dcc31ecbde 6 nSkcpbZcEkWqnLN7BVSvtznpj5Q (comment: CHECK interaction increases during cellular response to UV) ------- COMMENT: 45d907cccb67195b 2 87aLXY4GRmS7K2NrfSsNo69iCjs Comparison of mitochondria staining with GFP–Ung1 showed no detectable co-localization of Ung1 and mitochondria (Fig. 1B, right). Thus, these cellular localization studies indicate that fission yeast Ung1 is more similar to the nuclear form of human UNG as they both localized predominantly in the nucleus ------- COMMENT: 45e0cbb3de1695e3 1 r+yRgJ8tExZ3yvoayDWzkMPkbFg (comment: CHECK defect in sexual development in response to zinc or iron limitation) ------- COMMENT: 45e0cbb3de1695e3 11 r+yRgJ8tExZ3yvoayDWzkMPkbFg (comment: CHECK defect in sexual development in response to zinc or iron limitation) ------- COMMENT: 45f5ecd620515505 25 xiCwkRXNf7WtvyDNSfnycsI/ySQ ------- COMMENT: 45f5ecd620515505 28 AK7OsIhb7AHrYBRUaRmCgfIxdOs ------- COMMENT: 45f8955f7e6309ac 33 NhWnlpql1dZwuT7wigXSZh7XOKk (comment: CHECK not sure if this is the right term, sent a question) ------- COMMENT: 45ffde2cb6f9e878 17 2PgFnL2DO9EPyL5UIxRKNJHmtNM (comment: maybe not shown strongly in this paper but I'm trying to get the git genes annotated to this term because pka1 phosphorylates rst2 which excludes rst2 from the nucleus. rst2 when in the nucleus activates ste11 transcription.) ------- COMMENT: 465d15e06c53416f 1 HyfOIAVExHua/iE8FZp+dfyg4Qw no assembly of vesicles by (Because cell fu- sion completely fails when both partner cells lack fus1) ------- COMMENT: 465d15e06c53416f 3 NlvXQerb5XCOwuEkaIK4SgDWa14 (comment: CHECK Only when gpd1∆ is in h- cell) ------- COMMENT: 465d15e06c53416f 8 kY2gIbnvhMVTbuUflgKtusFik3E Fig. 6, A–C The density of vesicles was strongly reduced in the fus1Δ cells, whether this was h+ or h−, while WT h+ cells showed slightly higher vesicle density than h− cells, as in previous analysis () ------- COMMENT: 465d15e06c53416f 9 nMiztv7GSJ780IYV6Xdk27ziGU8 (comment: CHECK Stronger phenotype in h+ than h- cell) ------- COMMENT: 465d15e06c53416f 12 dJHzplfE/t/8UVmdNPFsBrtMIHI Fig. 6 E and Fig. S4 D. By LM, Myo52 and Exo84 also showed strong signal reduction in fus1Δ . ------- COMMENT: 465d15e06c53416f 17 bXmPw2anYkWQh87P4JAKMo9p6zA figure 5 ------- COMMENT: 465d15e06c53416f 18 bXmPw2anYkWQh87P4JAKMo9p6zA figure 5 ------- COMMENT: 465d15e06c53416f 21 kY2gIbnvhMVTbuUflgKtusFik3E Fig. 6, A–C The density of vesicles was strongly reduced in the fus1Δ cells, whether this was h+ or h−, while WT h+ cells showed slightly higher vesicle density than h− cells, as in previous analysis () ------- COMMENT: 465d15e06c53416f 22 PB1Z/Z5arzamDWu1zFkC3eP7XY0 Interestingly, the reduction in local secretion correlates with a strong loss of the wPM phenotype (Fig. 6, A and B; Fig. S4, E and F; and Fig. S5): only 4% of h+ fus1Δ cells (1/27) showed wPM, whereas 73% (16/22) and 20% (4/20) of h+ WT showed wPM in WT × WT and WT × fus1Δ crosses, respectively ------- COMMENT: 4666975359de04dd 24 FT16WLOj6Jj8vdBMRirzCEFL0J0 ------- COMMENT: 4666975359de04dd 36 W/H9kn37hnlJai5s8Ay+UMHIcUU ------- COMMENT: 466c4197f9cf80c6 1 9eVVpBObjczr/tnHoXoNKRxcPD0 (comment: suppresses ade6-M26 efficiently; suppresses ade6-M375 weakly) ------- COMMENT: 466c4197f9cf80c6 2 kqp9c96AEzyzye+1aix9zHUT5WI (comment: efficient suppression of ade6-M26; very poor suppression of ade6-M375) ------- COMMENT: 466c4197f9cf80c6 8 cjqAhu5M8618slgh5GFCusIpVoY (comment: CHECK high suppression of phenotype) ------- COMMENT: 466c4197f9cf80c6 11 cjqAhu5M8618slgh5GFCusIpVoY (comment: CHECK high suppression of phenotype) ------- COMMENT: 466c4197f9cf80c6 14 U9JKJymHVZfFR8MTYCFAEnggVQc (comment: low suppression of phenotype) ------- COMMENT: 466ed4e946c15c3f 1 g/uOTKfS7hcd3xQB648FmVy9bcA (comment: in response to queuosine incorporation into tRNA-Asp) ------- COMMENT: 466ed4e946c15c3f 3 lJqC38JUzlvepwW88DD0zi6C9ns (comment: CHECK waiting for go-ontology/issues/12536) ------- COMMENT: 468fa2c5da611313 7 H+t0SahSx/qhY5COfvQri9V6WVc (comment: Normal glucose consumption, but cell division is sensitive to low glucose condition) ------- COMMENT: 468fa2c5da611313 12 0TKZas876oF6sHQdzB3AUSuoxao However, in scs2Δ scs22Δ double deletion mutant cells, localization of both Cwh43–GFP and AHDL– mCherry at the plasma membrane disappeared, and, instead, accumulated in the cytoplasm ------- COMMENT: 468fa2c5da611313 13 /xrNpPH0SX0FDkh08G4Tmba88Eg Drastic changes in antioxidants, sugar derivatives, amino acid derivatives, organic acids, coenzyme A (CoA), and nucleotide derivatives. Most of these compounds are biomarkers for nutritional starvation (low-glucose or nitrogen-starvation). ------- COMMENT: 468fa2c5da611313 14 qZilXvqk3FVE/zS2LnR3fdBUo6g ------- COMMENT: 468fa2c5da611313 15 Qiqkvs1nIWjLd8On85nmAztXIcE ------- COMMENT: 468fa2c5da611313 17 iU74R7vnO2vJCY4wm7EdAOLh+nU ------- COMMENT: 468fa2c5da611313 18 iU74R7vnO2vJCY4wm7EdAOLh+nU ------- COMMENT: 468fa2c5da611313 20 PsmkiEpklHRYNAk16sdkMBP7XRo Synthetic growth defect between cwh43-G753R mutant and dga1Δ plh1Δ double deletion mutant. ------- COMMENT: 468fa2c5da611313 30 rXeC/yzEOdHTzxb/qXhQtRBZOQc Suppression of temperature sensitivity by 1.2M sorbitol ------- COMMENT: 468fa2c5da611313 31 rXeC/yzEOdHTzxb/qXhQtRBZOQc Suppression of temperature sensitivity by 1.2M sorbitol ------- COMMENT: 468fa2c5da611313 32 rXeC/yzEOdHTzxb/qXhQtRBZOQc Suppression of temperature sensitivity by 1.2M sorbitol ------- COMMENT: 468fa2c5da611313 33 GbdflLtzrOPDRHJ13KpbcyDAHZY Sorbitol addition partly suppresses beta-glucan accumulation in cwh43-G753R mutant cells ------- COMMENT: 468fa2c5da611313 34 B+GJ9e4NZgnspjDxkJEnoY+7twM Partial suppression of growth defect in the presence of sorbitol ------- COMMENT: 468fa2c5da611313 41 rXeC/yzEOdHTzxb/qXhQtRBZOQc Suppression of temperature sensitivity by 1.2M sorbitol ------- COMMENT: 468fa2c5da611313 78 UjMiTOsvFsHPu6fuazyR3zlLpT8 (comment: CHECK increased cellular dimethyl-histidine level during vegetative growth) ------- COMMENT: 468fa2c5da611313 102 skG08s4mDI+HA0BE0PXdyvBHyhU (comment: CHECK decreased cellular diphosphoglycerate level) ------- COMMENT: 468fa2c5da611313 109 PsmkiEpklHRYNAk16sdkMBP7XRo Synthetic growth defect between cwh43-G753R mutant and dga1Δ plh1Δ double deletion mutant. ------- COMMENT: 468fa2c5da611313 110 B+GJ9e4NZgnspjDxkJEnoY+7twM (comment: CHECK Partial suppression of growth defect in the presence of sorbitol) ------- COMMENT: 46ad29ab22f1f5e2 1 KG1iUNCItyiOTrCvaoL45tPUVoI To test these theoretical predictions, we measured the movements of the nucleus and of the SPB in a klp5Δ strain, which lacks the kinesin-8 motor Klp5 [Fig. 2(d)]. We observed that the nucleus is typically found farther away from the cell center in klp5Δ than in wild-type cells, as shown by the 1.7-fold larger standard deviation of the distribution of the nuclear position in klp5Δ cells, which is consistent with the prediction of the model [Figs. 2(b) ------- COMMENT: 46ad29ab22f1f5e2 2 ADrABSeML7GUj9xXKYlwzHwFnNE Similarly, the division plane was positioned more asymmetrically in klp5Δ cells than in wild-type cells [Fig. 2(g)]. ------- COMMENT: 46b68935ca658572 9 8cNUg237xGGaOWXN+u1yQbIxAhY (comment: Requires auto-phosphorylation to be restricted to cell tips -not restricted to cell tips for Pom1-6A and Pom1-KD alleles) ------- COMMENT: 46b68935ca658572 10 UJedVTUxtjtXkHe7NUNnMIvOx70 (comment: required for detachment from plasma membrane) ------- COMMENT: 46b68935ca658572 11 UJedVTUxtjtXkHe7NUNnMIvOx70 (comment: required for detachment from plasma membrane) ------- COMMENT: 46b68935ca658572 21 8cNUg237xGGaOWXN+u1yQbIxAhY (comment: Requires auto-phosphorylation to be restricted to cell tips -not restricted to cell tips for Pom1-6A and Pom1-KD alleles) ------- COMMENT: 46b68935ca658572 33 zj9m4VaLOYE7k8k73ICJ45i7Xbk (comment: I guess some might be false positives but Sophie said it should be ok.) ------- COMMENT: 46b68935ca658572 34 zj9m4VaLOYE7k8k73ICJ45i7Xbk (comment: I guess some might be false positives but Sophie said it should be ok.) ------- COMMENT: 46b68935ca658572 47 8cNUg237xGGaOWXN+u1yQbIxAhY (comment: Requires auto-phosphorylation to be restricted to cell tips -not restricted to cell tips for Pom1-6A and Pom1-KD alleles) ------- COMMENT: 46b68935ca658572 58 rjc4CaFvoFD1M5dwSSgGVOlK9G8 recombinant Pom1 N-terminus (MBP-Pom11–699) was able to bind directly to several, but not all, negatively charged lipids, namely phosphatidylserine, phosphatidylinositol phosphates, and cardiolipin in a protein-lipid overlay assay (Figure 2D). Phosphatidylserine and phosphatidylinositol phosphates are components of the plasma membrane. Cardiolipin is mostly found in the inner mitochondrial membrane, and so it is unclear whether this interaction exists in vivo. We also note that, probably due to its high global positive charge (+15.5 for MBP-Pom11–699, +25 for Pom11–699 at pH 7), MBP-Pom11–699 bound the nitrocellulose membrane, resulting in significant background. Together, these experiments suggest that Pom1 directly associates with lipids at the plasma membrane through its basic region." ------- COMMENT: 46c89f342f117ca5 1 4NQYTKAy1IKBH8AFZxx+0DvBcAk Homothalic pak2∆ partners exhibit fusion efficiency decrease of ~20% as compared to wildtype partner fusion. Homothalic pak2∆ cells undergo transient fusion with frequency of ~10%, which is absent in wildtype matings. ------- COMMENT: 46c89f342f117ca5 3 AGXmkEohf8/XK6m379+NZymkl2Q fig 1 II ------- COMMENT: 46c89f342f117ca5 5 bkBq+hI4iIXYHOzrkND78qfmNk8 (comment: Regulation of asymmetric gene expression from parental genomes Factor that regulates differential gene expression of homologous parental gene copies) ------- COMMENT: 46c89f342f117ca5 8 bwIeCQ0l8aAq8cu2v9okemXZM1E (comment: M-cells) ------- COMMENT: 46c89f342f117ca5 9 Utyo4qwI0hRVtDQlNG4SJbyoLRI (comment: CHECK P-cells (rapid) M-cells (delayed)) ------- COMMENT: 46c89f342f117ca5 10 aJhMk9aERfGqLaXozE1F/pYbnmY Extended Data Fig. 3b, Supplementary Video 5a ------- COMMENT: 46c89f342f117ca5 12 w1ODRKoUkmybaqV5iWE+C6KD6zU (comment: CHECK GO:0140538 +name: negative regulation of conjugation with zygote https://github.com/geneontology/go-ontology/issues/16329) ------- COMMENT: 46c89f342f117ca5 17 /9F5r75h9kFajjt4mHpJrErtKnI Fig. 1a, type IIIa ------- COMMENT: 46c89f342f117ca5 18 GTppOKl1zQIj85Qvfy4JYAIrygI Fig. 1a, type IIIb ------- COMMENT: 46c89f342f117ca5 19 sVrZWUeY0kH6fS+IfoxkusRRgZw (comment: CHECK abolished karyogamy with transient cytogamy -this is a bit like twin haploid meiosis? should be siblings? also looks like karyogamy failure) ------- COMMENT: 46c89f342f117ca5 20 sVrZWUeY0kH6fS+IfoxkusRRgZw (comment: CHECK abolished karyogamy with transient cytogamy -this is a bit like twin haploid meiosis? should be siblings? also looks like karyogamy failure) ------- COMMENT: 46c89f342f117ca5 21 Ocx+iB/95EBAbk5q7f3QrSYRqPw Figure 1c ------- COMMENT: 46c89f342f117ca5 23 QTIpNxIgokFSs3aDZiWBeswVHTQ Extended data figure 1F (comment: dominant over shk2 downstream sporulation phenotypes) ------- COMMENT: 46c89f342f117ca5 24 J8L6RRd/ton2PpV8kPGJwe2qAW0 Fig. 1g ------- COMMENT: 46c89f342f117ca5 25 GTppOKl1zQIj85Qvfy4JYAIrygI Fig. 1a, type IIIb ------- COMMENT: 46c89f342f117ca5 26 4ncaW1LVB8JCHuPYXwo30vABZIA (comment: CHECK ******abolished in M cells) Fig. 2a, Supplementary Video 5, Fig. 2b, Supplementary Video 2a Importantly, mei3 was also asymmetrically expressed in WT...zygotes first express the meiotic inducer Mei3 from the P genome. ------- COMMENT: 46c89f342f117ca5 28 C637g1HIQjjmG7mkbFCDGhcwT0I (comment: CHECK go-ontology/issues/16327) ------- COMMENT: 46c89f342f117ca5 29 jbKGq/daU7sjaDS7Eo01CSyUmCE (comment: CHECK *******to nucleus of opposite mating type cell******) ------- COMMENT: 46c89f342f117ca5 30 n8S5rP9UFaFQvddVvzotPJNltRU Fig. 3d, Supplementary Video 5b ------- COMMENT: 46c89f342f117ca5 31 DlQ9xR6kEGsEuu+IDzQv0R7f43w Fig. 4a, Extended Data Fig. 4c, Supplementary Video 7b ------- COMMENT: 46c89f342f117ca5 32 DlQ9xR6kEGsEuu+IDzQv0R7f43w Fig. 4a, Extended Data Fig. 4c, Supplementary Video 7b ------- COMMENT: 46c89f342f117ca5 33 DlQ9xR6kEGsEuu+IDzQv0R7f43w Fig. 4a, Extended Data Fig. 4c, Supplementary Video 7b ------- COMMENT: 46c89f342f117ca5 34 LI1k4zhAUsfcwR19oaO8l2u3ciE Fig. 2c (comment: never ending search for mating partner) ------- COMMENT: 46c89f342f117ca5 36 BavQ8iFBTwMMqGGruSSsnFZ1fjg (comment: never ending search for mating partner by P cell) ------- COMMENT: 46c89f342f117ca5 37 fNre13qiVoIqtVsin43uBW7dJRQ (comment: in M-cell) ------- COMMENT: 46c89f342f117ca5 39 SXqTgtorqNbS+hHtBsaam3smBSM Extended Data Fig. 5e, f, Supplementary Video 9; see also ref. 1 ------- COMMENT: 46c89f342f117ca5 40 SXqTgtorqNbS+hHtBsaam3smBSM Extended Data Fig. 5e, f, Supplementary Video 9; see also ref. 1 ------- COMMENT: 46c89f342f117ca5 41 ha6t38k9K3unAnUdJYNrwfb8em4 (comment: Regulation of asymmetric gene expression from parental genomes) ------- COMMENT: 46c89f342f117ca5 42 ha6t38k9K3unAnUdJYNrwfb8em4 (comment: Regulation of asymmetric gene expression from parental genomes) ------- COMMENT: 46c89f342f117ca5 43 bkBq+hI4iIXYHOzrkND78qfmNk8 (comment: Regulation of asymmetric gene expression from parental genomes Factor that regulates differential gene expression of homologous parental gene copies) ------- COMMENT: 4710b4a242847ef4 1 /P1IfY1NcKzb4aBXDVHj/v/K/ew (comment: RNA-IP interaction) ------- COMMENT: 4710b4a242847ef4 2 /P1IfY1NcKzb4aBXDVHj/v/K/ew (comment: RNA-IP interaction) ------- COMMENT: 472cd1acc285281c 25 5/4lLmypV5ED2tcDexZ94Bx+aoI (comment: same as chk1delta alone) ------- COMMENT: 472cd1acc285281c 26 5/4lLmypV5ED2tcDexZ94Bx+aoI (comment: same as chk1delta alone) ------- COMMENT: 472cd1acc285281c 32 V8fcFA8KwouLR6JuTO0WyLYM32A (comment: same as exo1delta alone) ------- COMMENT: 472cd1acc285281c 33 V8fcFA8KwouLR6JuTO0WyLYM32A (comment: same as exo1delta alone) ------- COMMENT: 472cd1acc285281c 34 tzWFWHcMu4olGbBv3Is6nNbc8Uw (comment: same as rad2delta alone) ------- COMMENT: 472cd1acc285281c 35 tzWFWHcMu4olGbBv3Is6nNbc8Uw (comment: same as rad2delta alone) ------- COMMENT: 472cd1acc285281c 52 ApR3JIxLNoSAveGq7Iv6AwrBi4Q (comment: gel electrophoresis + southern blot) ------- COMMENT: 473f08550050fe95 1 Dpv9t8hSV6ConPSfcqq8ULYXvZo Figure 1 and 2 We conclude that it is the new SPB that fails to activate and insert into the nuclear envelope in cut12.1 mutants. ------- COMMENT: 473f08550050fe95 3 Dpv9t8hSV6ConPSfcqq8ULYXvZo Figure 1 and 2 We conclude that it is the new SPB that fails to activate and insert into the nuclear envelope in cut12.1 mutants. ------- COMMENT: 473f08550050fe95 4 iZriTHg4usrSJ44TSVUcNq31MMg gapped membrane distortions in the nuclear envelope of cut12.1 cells at 36°C (Fig. 3, A–D). 7D ------- COMMENT: 473f08550050fe95 5 F5PSWWL9HL+F2HKNJ6gNcA8rCv4 (Fig. 4 C and Video 3) ------- COMMENT: 473f08550050fe95 6 5Geqgb5B3rRxLJsyE9fq0xBdZ54 our ability to identify cells in which an active SPB had apparently lost its association with the membrane completely and fallen into the middle of the nucleus (Fig. 3 E) and the proximity of the SPB to these gaps in the membrane (Fig. 3, A–C). ------- COMMENT: 473f08550050fe95 7 5Geqgb5B3rRxLJsyE9fq0xBdZ54 our ability to identify cells in which an active SPB had apparently lost its association with the membrane completely and fallen into the middle of the nucleus (Fig. 3 E) and the proximity of the SPB to these gaps in the membrane (Fig. 3, A–C). ------- COMMENT: 473f08550050fe95 13 6PkinWSMVRaIoCVaE+wRxvcJNts (comment: CHECK inviable->viable) ------- COMMENT: 473f08550050fe95 14 KpK+U8p67Cv2tTg2rJdIy/oqjT4 (comment: CHECK monopolar) ------- COMMENT: 473f08550050fe95 15 KpK+U8p67Cv2tTg2rJdIy/oqjT4 (comment: CHECK monopolar) ------- COMMENT: 473f08550050fe95 20 eDhsgl/8dEF1mzQVf3pZV+lOxwk Surprisingly, in 50% (n = 79) of the cut12.1 cells that successfully completed mitosis, an efflux of the NLS-GFP–-Gal marker accompanied mitotic commitment (Fig. 7, C and D). ------- COMMENT: 473f08550050fe95 21 r1VrsOxUySsDeGBJ5u5FLIer/Xs (comment: CHECK insertion) ------- COMMENT: 477003fc9090fdf9 2 XPnM32shLX68Eo2rR66Yrm1uKgQ fig 1c ------- COMMENT: 477003fc9090fdf9 3 XPnM32shLX68Eo2rR66Yrm1uKgQ fig 1c ------- COMMENT: 477003fc9090fdf9 4 75o05rEXRjLcpzLMNhPlniTgYlA (comment: Fin1 binds Byr4. (A) Fin1 failed to associate with SPBs when the SIN was either inactive or hyperactive.) ------- COMMENT: 477003fc9090fdf9 5 IwiaV2qDwpZ6HvMwYTzRDN67+jw Fin1 binds Byr4. (A) Fin1 failed to associate withSPBs when the SIN was either inactive or hyperactive. Fin1 failed to bind to the SPB when byr4+ was deleted (Table 1). ------- COMMENT: 477003fc9090fdf9 7 Z3dy3L2mkSq2Yr+OEf7UEb+Ag08 Fin1 failed to bind to the SPB when byr4+ was deleted (Table 1). ------- COMMENT: 477003fc9090fdf9 8 w4G7F3iG/jVqUrRgoN9PHPTN+gc We draw two conclu- sions from these data; first, association of Fin1 with the SPB requires activation of the SIN; second, recruitment of Fin1 to the SPB requires the SIN inhibitor Byr4, with which it interacts. ------- COMMENT: 477003fc9090fdf9 9 JAhiAQgIbDOF3J5J1DFMPX0dug4 (comment: CHECK is this the correct term?) ------- COMMENT: 4794f2ac68f1b5ba 22 2zKckuWh3Lpq+zJjYTy0+M81UeA Figure 1A Figure 5D ------- COMMENT: 4794f2ac68f1b5ba 23 5SLq4jjUZx4o2fqO4NJ9786gpN0 Figure S2C ------- COMMENT: 4794f2ac68f1b5ba 26 eXWPNIslufPlAy5Ve5KCTnJvMXM Figure S2A ------- COMMENT: 4794f2ac68f1b5ba 27 eXWPNIslufPlAy5Ve5KCTnJvMXM Figure S2A ------- COMMENT: 4794f2ac68f1b5ba 28 eXWPNIslufPlAy5Ve5KCTnJvMXM Figure S2A ------- COMMENT: 4794f2ac68f1b5ba 29 +9U0KAAWhidPxfr8K6AizO375Zg Figure S2B ------- COMMENT: 4794f2ac68f1b5ba 31 +9U0KAAWhidPxfr8K6AizO375Zg Figure S2B ------- COMMENT: 4794f2ac68f1b5ba 32 +9U0KAAWhidPxfr8K6AizO375Zg Figure S2B ------- COMMENT: 47b885e9317af565 1 dGmiHh7vEE8m/5DlQmWVBZ4hReE (comment: SO:0000407 = 18s rRNA, the genes are the 18s genes of the "correct length" so I guess we want them in there if we want to be able to make the "connections" from the info in the database? Though I guess this regions isnt properly sequenced..) ------- COMMENT: 47b885e9317af565 2 g+0r9NZwUXeJaGR433TWa7gu1MI (comment: They couldn't make the knockout in haploid, and diploid inviable, hence inferring inviable vegetative rather than more general "inviable cell pop" or inviable spore pop) ------- COMMENT: 47cceba0fa44ef05 1 PD/0cGNzZIA7yhoXP3P4/tDE3vk Figure 1,2 ------- COMMENT: 47cceba0fa44ef05 2 WTp4nFTY3YQcisqSVbePLJMSZp0 figure 2C (comemnt: vw: not by sty1) ------- COMMENT: 47cceba0fa44ef05 3 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: 47cceba0fa44ef05 4 xxLbYu0obs+Sf3/zvXw6d0KgKQA Figure 3 (comemnt: vw severity 23.4 micron) ------- COMMENT: 47cceba0fa44ef05 5 GNsYBYqfJUtdVV1jeA9BVBIaLP4 Figure 3 (comemnt: vw: severity 20.2 micron) ------- COMMENT: 47cceba0fa44ef05 6 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 47cceba0fa44ef05 7 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 47cceba0fa44ef05 10 9BSDRu77gCcpiKi803iu7V3zM+k Table 2 ------- COMMENT: 47cceba0fa44ef05 11 RaPiJeE4LAzmUvVItFnpkPMvmGY figure 3b ------- COMMENT: 47cceba0fa44ef05 12 RaPiJeE4LAzmUvVItFnpkPMvmGY figure 3b ------- COMMENT: 47cceba0fa44ef05 15 eltegc2JSEetR3GiNjSB6pAVA+Q (comment: vw: sty1-atf1 pathway) ------- COMMENT: 47cceba0fa44ef05 16 eltegc2JSEetR3GiNjSB6pAVA+Q (comment: vw: sty1-atf1 pathway) ------- COMMENT: 47cceba0fa44ef05 17 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 47cceba0fa44ef05 18 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 47cceba0fa44ef05 19 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 47cceba0fa44ef05 20 5Leuj0nQtQ/oH58v8XLkYXSDMog Figure 6B ------- COMMENT: 47cceba0fa44ef05 21 PAlSCsNJoWMQnn/zm8kYnyn7T7s figure 7 ------- COMMENT: 47cceba0fa44ef05 22 PAlSCsNJoWMQnn/zm8kYnyn7T7s figure 7 ------- COMMENT: 47cceba0fa44ef05 23 PAlSCsNJoWMQnn/zm8kYnyn7T7s figure 7 ------- COMMENT: 47cceba0fa44ef05 24 WBIx3dXja9Ph+VOdp+ZIdOwHlz8 Figure1/2 ------- COMMENT: 47cceba0fa44ef05 25 9BSDRu77gCcpiKi803iu7V3zM+k table 2 ------- COMMENT: 47e7d9844090c3e4 1 3gsN2LfW7P0wBJDta8Nk01Zqtc8 (comment: APC-Ste9 dependent protein destruction/11365/) I didn't do a phenotype for this because they don't show a WT scanario. I used the ubiquitin-dependent term becasuethis is whet they were testing as we already know that this decgradation is APC/protiesome dependnet. I know its a bit of a stretch) ------- COMMENT: 47e7d9844090c3e4 3 5Pxy7RMu8wqP9KPeJ60HtI/XTQA fig 3 (comment: CHECK cdc25-22 block and release) ------- COMMENT: 47e7d9844090c3e4 5 IdmW4sOAVlO6J+NBlqHUhbY1Jdk fig3 ((comment: CHECK cdc25-22 block and release) ------- COMMENT: 47e7d9844090c3e4 7 IdmW4sOAVlO6J+NBlqHUhbY1Jdk fig3 (comment: cdc25-22 block and release) ------- COMMENT: 47e7d9844090c3e4 29 Rnd9WT5/MzJ78H9Dxl/ZFgoo+Ws (comment: APC-SLP1) ------- COMMENT: 47e7d9844090c3e4 30 WIQobKVmOSwF4pnw3zyHiiYk2Lk fig 1a ------- COMMENT: 47e7d9844090c3e4 31 WIQobKVmOSwF4pnw3zyHiiYk2Lk fig 1a ------- COMMENT: 47e7d9844090c3e4 32 OM9/IdHtw3bg82HIl4sEasfS8Ew fig 1b ------- COMMENT: 48003915c37d2609 1 1CqW0nPLwZiLayniON5BW6g4uOY (Figure S2F) ------- COMMENT: 48003915c37d2609 3 aUV98GcIsD+KH7e6C7Srlo6w0Kg we observed derepression of several subtelomeric genes (Figures 2E and S2G). ------- COMMENT: 48003915c37d2609 4 Kt5kA6cHyo68SvSq59mHetTqs0Q (Figure 2D) reduced heterochromatin spreading at mating-type and subtelomeric heterochromatin ------- COMMENT: 48003915c37d2609 7 1DY7BaRVor2tXTojnE3urAhvov0 (Figure 1B) In contrast, there was a substantial reduction of H3K9me2 at the mating-type locus, in agreement with a previous study (Holla et al., 2020) (Figure S1A), and at the subtelomeres ------- COMMENT: 48003915c37d2609 9 ycdU2unJSSS5aIWqaUsaaUVC/mM (Figures 1B, 1C, S1D) only a subtle change of H3K9me2 at pericentromere ------- COMMENT: 48003915c37d2609 10 9NXYNhZiM+NshupDoLZDGoBOvsw (Figure 2C) Conversely, in the pob3Δ strain, the orange reporter was also fully derepressed in the majority of cells, yet the green reporter remained silenced or mildly derepressed. This result implies that pob3Δ cells have a heterochromatin spreading defect ------- COMMENT: 48003915c37d2609 11 Ybjn3p8HKjfOcEOQ/AEgwsCBHNA Nonetheless, the pob3Δ mutant exhibited increased incorporation of H3-T7 at the TEL1L region (Figure 4G), implying that the H3 turnover rate is increased at subtelomeric heterochromatin. ------- COMMENT: 48003915c37d2609 12 wf185u4DRDkg7Sbpsn3gLe6+2NY Figure 4E ------- COMMENT: 48003915c37d2609 13 wf185u4DRDkg7Sbpsn3gLe6+2NY Figure 4E ------- COMMENT: 48003915c37d2609 14 ca6Cx7ZqF85D+XkKlZVyKa70Cxo Intriguingly, while H3K9me2 levels were unaltered in spt16–1, H3K9me3 levels were reduced at several loci at the TEL1L subtelomeric region (i.e., SPAC212.09c, SPAC212.08c, and SPAC212.06c; compare Figure 4B with 4A) ------- COMMENT: 48003915c37d2609 17 vq19PdHIkRhcOwAAJAgLGv16xmQ synthetic defect in the silencing of dg and tlh1/2 transcripts (Figure 4C) ------- COMMENT: 48003915c37d2609 21 wf185u4DRDkg7Sbpsn3gLe6+2NY Figure 4E ------- COMMENT: 48003915c37d2609 22 wf185u4DRDkg7Sbpsn3gLe6+2NY Figure 4E ------- COMMENT: 48003915c37d2609 23 FWjIlrjDukp4B3pr72ZzhMSmXGc (Figure 1F) ------- COMMENT: 48003915c37d2609 24 FWjIlrjDukp4B3pr72ZzhMSmXGc (Figure 1F) ------- COMMENT: 48003915c37d2609 25 cSsWi2YzmBkWr64RYoJZouhhS2Y (comment: vw: changed from decreased to normal because look WT?) ------- COMMENT: 48003915c37d2609 27 sxQS+dzwYSeE+7P8+Df4ZNzyLcQ (Figure 1 G) ------- COMMENT: 48003915c37d2609 28 sxQS+dzwYSeE+7P8+Df4ZNzyLcQ (Figure 1 G) ------- COMMENT: 48003915c37d2609 29 CZGXy4JC+FzgS/1iG6VyW9eR3B8 (fig 1G ) (comment: vw: changed to increased -compared to WT) ------- COMMENT: 48003915c37d2609 30 46zJ/1ZJelw3ugk9AxNU955mNRc (fig 1G ) (comment: vw: changed to increased -compared to WT) ------- COMMENT: 48003915c37d2609 43 y2Co56THIqLVv4J7DkMzzi1cXTI Moreover, epe1Δ reduced the expression of several subtelomeric genes in pob3Δ, suggesting that it also counteracts heterochromatin spreading (Figure 3G) ------- COMMENT: 48003915c37d2609 44 U9mNl2qe/4giCDwh9AuymSn4MSc Partial suppression of pob3∆ silencing phenotype. ------- COMMENT: 48003915c37d2609 45 S8hZHPTiABoDu69R6KiFiP8hmuI To test this more directly, we performed the HSS assay in the double spt16–1epe1Δ mutant. Indeed, heterochromatin spreading was completely restored (Figure 3H, compare with Figure 2D) ------- COMMENT: 48003915c37d2609 47 U0mutIu54vR7Iqp8z3JNMBygu5I (Figures 1E and S1D) we found increased signals of elongating RNAPII (RNAPII Ser2P) and H2B ubiquitination (H2Bub), a histone mark associated with active transcription ------- COMMENT: 48003915c37d2609 48 B38nmilXkYzTl0MFN+EaZdIzGh4 (Figure S1E) ------- COMMENT: 48003915c37d2609 50 11cgACOjaw/4IqISagRTylTrDOw Figures S2C–S2E in chromatin/euchromatin ------- COMMENT: 48003915c37d2609 51 L3Gf7sN4HDZ2svLTLpTLfk+unt0 (comment: partial supession -both deletion alleles) ------- COMMENT: 48003915c37d2609 52 ZxC4Bg9ZQGtwXpcLuL6FFPKiLAk Although degradation of Epe1 still occurs in pob3Δ in S phase, we found increased steady-state levels of Epe1 in cycling cells (Figure S3A). ------- COMMENT: 48003915c37d2609 54 vq19PdHIkRhcOwAAJAgLGv16xmQ synthetic defect in the silencing of dg and tlh1/2 transcripts (Figure 4C) ------- COMMENT: 48003915c37d2609 55 b7VeT8rwEEkUmpjTSAm9uMi/gOg found reduced Swi6 binding in spt16–1 at subtelomeric genes close to the heterochromatin boundary (SPAC212.12, SPAC212.06c; Figure 4D). ------- COMMENT: 48003915c37d2609 56 x5fuZW63tHhFgx5Dz7mONAfoOKA (Figures S4A–S4C) Histone H3 ChIP-seq revealed a small but reproducible reduction of H3 at subtelomeres in pob3Δ ------- COMMENT: 48003915c37d2609 61 Kt5kA6cHyo68SvSq59mHetTqs0Q (Figure 2D) reduced heterochromatin spreading at mating-type and subtelomeric heterochromatin ------- COMMENT: 48003915c37d2609 62 U0mutIu54vR7Iqp8z3JNMBygu5I (Figures 1E and S1D) we found increased signals of elongating RNAPII (RNAPII Ser2P) and H2B ubiquitination (H2Bub), a histone mark associated with active transcription ------- COMMENT: 48003915c37d2609 63 aUV98GcIsD+KH7e6C7Srlo6w0Kg we observed derepression of several subtelomeric genes (Figures 2E and S2G). ------- COMMENT: 480394fd534ef4bd 2 XPnM32shLX68Eo2rR66Yrm1uKgQ fig 1C ------- COMMENT: 480394fd534ef4bd 3 XPnM32shLX68Eo2rR66Yrm1uKgQ fig 1C ------- COMMENT: 480394fd534ef4bd 4 UWHJjEgJsonSclsHnty9Czz/AVQ Fig 2A,B ------- COMMENT: 480394fd534ef4bd 9 vx1Q0X0eTQRpqEd4GUjtM0e6TVc (Fig 3B) ------- COMMENT: 480394fd534ef4bd 10 vx1Q0X0eTQRpqEd4GUjtM0e6TVc (Fig 3B) ------- COMMENT: 480394fd534ef4bd 11 vx1Q0X0eTQRpqEd4GUjtM0e6TVc (Fig 3B) ------- COMMENT: 480394fd534ef4bd 14 bXmPw2anYkWQh87P4JAKMo9p6zA Figure 5 ------- COMMENT: 480394fd534ef4bd 15 qoAZt0Gco/33JhPBHNpV5/VI6hE supplementary Fig S6 online ------- COMMENT: 482d360415391124 75 UOVgLoN1OxQD4THO9+G93OpBqnY abnormal septum forms on the surface from one side of the cell and then extends in a disor- ganized manner into the interior ------- COMMENT: 4843d60805e30d72 1 dDZQGEks0BzDdquYlDJF4LTr/m0 (comment: Red1192–236 and Iss101–45) In agreement with the recent Y2H analysis of the MTREC complex interactions14, we detected strong interactions with Ars2, Iss10 and Mtl1 as well as between Iss10 and Mmi1 (Fig. 1b and Supplementary Fig. 1a, b). ------- COMMENT: 4843d60805e30d72 2 3UkIKqzStcEEzOVP1MUv0fyH2ts In agreement with the recent Y2H analysis of the MTREC complex interactions14, we detected strong interactions with Ars2, Iss10 and Mtl1 as well as between Iss10 and Mmi1 (Fig. 1b and Supplementary Fig. 1a, b). ------- COMMENT: 4843d60805e30d72 3 3UkIKqzStcEEzOVP1MUv0fyH2ts In agreement with the recent Y2H analysis of the MTREC complex interactions14, we detected strong interactions with Ars2, Iss10 and Mtl1 as well as between Iss10 and Mmi1 (Fig. 1b and Supplementary Fig. 1a, b). ------- COMMENT: 4843d60805e30d72 4 anuKXMgmsYXgp0Rpw0yPTkDGNIU In addition, our Y2H assay revealed an unexpected Red1 self-association (Fig. 1c). ------- COMMENT: 4843d60805e30d72 5 FMcS8M3iVYAGecZRFmgiK+UL+jQ While A198E retained the WT level of binding (Fig. 2i, lanes 3 and 7), the L205R and F215R mutants no longer bound Iss10 (Fig. 2i, lanes 4, 5, 8 and 9). ------- COMMENT: 4843d60805e30d72 6 FMcS8M3iVYAGecZRFmgiK+UL+jQ While A198E retained the WT level of binding (Fig. 2i, lanes 3 and 7), the L205R and F215R mutants no longer bound Iss10 (Fig. 2i, lanes 4, 5, 8 and 9). ------- COMMENT: 4843d60805e30d72 7 WvRHyyunMZ7dqVkZATvAluTpFiU Using these cells, reciprocal co-immunoprecipitation experiments showed that the Red1-L205R mutation also compro- mised Red1 association with Iss10 in S. pombe (Fig. 3a and Supple- mentary Fig. 3a). ------- COMMENT: 4843d60805e30d72 8 kyefs51AmDFrt1oozhJUrXPjSDs While red1Δ cells showed only a moderate growth defect on solid rich medium at 30°C, this defect was more pronounced at lower temperature of 25 or 18°C (Supplementary Fig. 3b), as published previously1 ------- COMMENT: 4843d60805e30d72 9 kyefs51AmDFrt1oozhJUrXPjSDs While red1Δ cells showed only a moderate growth defect on solid rich medium at 30°C, this defect was more pronounced at lower temperature of 25 or 18°C (Supplementary Fig. 3b), as published previously1 ------- COMMENT: 4843d60805e30d72 10 V/cRqq/xCS9L+F2KCLa27D9CQ78 While red1Δ cells showed only a moderate growth defect on solid rich medium at 30°C, this defect was more pronounced at lower temperature of 25 or 18°C (Supplementary Fig. 3b), as published previously14,34 or when the cells were grown on minimal medium (Supplementary Fig. 3c). ------- COMMENT: 4843d60805e30d72 11 txaONWCToY48jqutiLAADlLFEP8 Importantly, red1-L205R cells showed a simi- lar growth defect, when grown on minimal medium, suggesting that Red1-Iss10 interaction can be important for Red1-dependent cell growth function depending on the environmental conditions (Fig. 3b). ------- COMMENT: 4843d60805e30d72 12 l2/WjYUxLzMegt+7uhb1GksJLDs We observed that the red1-L205R mutation had an effect on both Iss10 and Red1 localization by, respectively, inducing the disappearance of Iss10 nuclear foci and the increase in the number of Red1 foci per nucleus (Fig. 3c–e) ------- COMMENT: 4843d60805e30d72 13 l2/WjYUxLzMegt+7uhb1GksJLDs We observed that the red1-L205R mutation had an effect on both Iss10 and Red1 localization by, respectively, inducing the disappearance of Iss10 nuclear foci and the increase in the number of Red1 foci per nucleus (Fig. 3c–e) ------- COMMENT: 4843d60805e30d72 14 SjvAhsTNjGpEZJYOyLSfQRy1IxA These findings indicate that the interaction of Iss10 with Red1 is required for Iss10 recruitment to Red1 nuclear foci and suggest that it may promote the clustering of Red1 nuclear foci. ------- COMMENT: 4843d60805e30d72 15 l2/WjYUxLzMegt+7uhb1GksJLDs We observed that the red1-L205R mutation had an effect on both Iss10 and Red1 localization by, respectively, inducing the disappearance of Iss10 nuclear foci and the increase in the number of Red1 foci per nucleus (Fig. 3c–e) ------- COMMENT: 4843d60805e30d72 16 l2/WjYUxLzMegt+7uhb1GksJLDs We observed that the red1-L205R mutation had an effect on both Iss10 and Red1 localization by, respectively, inducing the disappearance of Iss10 nuclear foci and the increase in the number of Red1 foci per nucleus (Fig. 3c–e) ------- COMMENT: 4843d60805e30d72 17 l2/WjYUxLzMegt+7uhb1GksJLDs We observed that the red1-L205R mutation had an effect on both Iss10 and Red1 localization by, respectively, inducing the disappearance of Iss10 nuclear foci and the increase in the number of Red1 foci per nucleus (Fig. 3c–e) ------- COMMENT: 4843d60805e30d72 18 l2/WjYUxLzMegt+7uhb1GksJLDs We observed that the red1-L205R mutation had an effect on both Iss10 and Red1 localization by, respectively, inducing the disappearance of Iss10 nuclear foci and the increase in the number of Red1 foci per nucleus (Fig. 3c–e) ------- COMMENT: 4843d60805e30d72 19 l2/WjYUxLzMegt+7uhb1GksJLDs We observed that the red1-L205R mutation had an effect on both Iss10 and Red1 localization by, respectively, inducing the disappearance of Iss10 nuclear foci and the increase in the number of Red1 foci per nucleus (Fig. 3c–e) ------- COMMENT: 4843d60805e30d72 20 l2/WjYUxLzMegt+7uhb1GksJLDs We observed that the red1-L205R mutation had an effect on both Iss10 and Red1 localization by, respectively, inducing the disappearance of Iss10 nuclear foci and the increase in the number of Red1 foci per nucleus (Fig. 3c–e) ------- COMMENT: 4843d60805e30d72 21 l2/WjYUxLzMegt+7uhb1GksJLDs We observed that the red1-L205R mutation had an effect on both Iss10 and Red1 localization by, respectively, inducing the disappearance of Iss10 nuclear foci and the increase in the number of Red1 foci per nucleus (Fig. 3c–e) ------- COMMENT: 4843d60805e30d72 22 l2/WjYUxLzMegt+7uhb1GksJLDs We observed that the red1-L205R mutation had an effect on both Iss10 and Red1 localization by, respectively, inducing the disappearance of Iss10 nuclear foci and the increase in the number of Red1 foci per nucleus (Fig. 3c–e) ------- COMMENT: 4843d60805e30d72 23 l2/WjYUxLzMegt+7uhb1GksJLDs We observed that the red1-L205R mutation had an effect on both Iss10 and Red1 localization by, respectively, inducing the disappearance of Iss10 nuclear foci and the increase in the number of Red1 foci per nucleus (Fig. 3c–e) ------- COMMENT: 4843d60805e30d72 25 KPIsCr6pBj4aL9NIQeqKbF4tzXI In Strep-tag pull down assays, both mutations (K483D, F490D) essentially disrupted the Red1 binding (Supplementary Fig. 7d, lanes 3, 4). ------- COMMENT: 4843d60805e30d72 26 3mTycolNZzoZszgDUlsaUsMM+Z4 Using Y2H assays, we could show that the Red1 E32R mutation is sufficient to prevent the interaction with Ars2 in the context of full-length proteins (Fig. 5f). mportantly, while Red1-TAP and Ars2-GFP interact, as expected, this interaction was lost in cells expressing Red1-E32R-TAP (Fig. 5g). ------- COMMENT: 4843d60805e30d72 27 aoIXP13moOj/aXLF7Oe0fVOX5YY A similar growth defect was visible for the red1-E32R-TAP mutant cells grown on minimal medium, suggesting that Red1-Ars2 interaction, depending on the growth conditions, can be important for the normal growth of the cell population (Fig. 5h). ------- COMMENT: 4843d60805e30d72 28 M/zQ+7wvZDvD5OgnXeP8ydZHVkQ However, no significant changes were observed for several meiotic mRNAs and PROMPTs/CUTs MTREC targets (Supplementary Fig. 8b, c) ------- COMMENT: 4843d60805e30d72 29 M/zQ+7wvZDvD5OgnXeP8ydZHVkQ However, no significant changes were observed for several meiotic mRNAs and PROMPTs/CUTs MTREC targets (Supplementary Fig. 8b, c) ------- COMMENT: 4843d60805e30d72 30 ycaLk1TvlbNm28mNT1DdTR67XQ4 We examined the possible effect of red1-E32R mutation on pho1 and byr2 mRNAs. However, no significant changes in pho1 and byr2 mRNA levels were observed between wild-type and red1-E32R mutant cells (Supplementary Fig. 8d). ------- COMMENT: 4843d60805e30d72 31 ycaLk1TvlbNm28mNT1DdTR67XQ4 We examined the possible effect of red1-E32R mutation on pho1 and byr2 mRNAs. However, no significant changes in pho1 and byr2 mRNA levels were observed between wild-type and red1-E32R mutant cells (Supplementary Fig. 8d). ------- COMMENT: 4843d60805e30d72 32 QaOkICptzS3gj7VxALREKVYm3/o Interestingly, quantification of the intensity of the signal within each nucleus showed a reduction of Ars2-GFP nuclear signal in red1-E32R mutant cells compared to wild- type cells, suggesting that some of Ars2-GFP proteins diffuse to the cytoplasm (Supplementary Fig. 8e). ------- COMMENT: 488ebe84ae41b570 1 02PmpTUEB4oOJsIupzDTlqh3UKg Activation of Cds1 requires the recruitment by Mrc1 and subsequent phosphorylation of threonine 11 by Rad3. Phosphorylation of threonine 11 promotes homodimerization of Cds1, which facilitates the autophosphorylation of threonine 328 in the kinase domains of the dimer partners. Phosphorylation of threonine 328 directly activates Cds1. The kinase activity of Cds1 is low during a normal cell cycle. However, it increases dramatically during a perturbed S phase. ------- COMMENT: 48962b9e331018c2 1 kOgQrhrqrMqJOVTkESNX3IFMNGc A ts allele of sap1 causes a reduction in Tf1 integration at permissive temperature ------- COMMENT: 48962b9e331018c2 2 U0NC7kPHWI5Il6xFT/SPdy2SOas At 25 deg C the Sap1-1 allele caused a 10-drop in Tf1-neo transposition as detected by quantitative assay. ------- COMMENT: 4899d62746d85c40 1 UmOpUdRzmr2aBIUmP84IC9Z7REc FIgure 1. These results indicate that the error correction mechanism decreases bi-oriented attachment of sister chromatids in the presence of chiasmata, but conversely increases bi-oriented attachment (thereby decreasing mono-oriented attachment) in the absence of chiasmata. In our previous study, the equational segregation frequencies of cen1 were somewhat higher in the rec12Δ background [28], although the reason for this is unknown. However, the mad2Δ mutation similarly decreased equational segregation, being consistent with our current results. ------- COMMENT: 4899d62746d85c40 2 RgWkk2YjtcEPOfQh0O0cjUWMN2I FIgure 1. Decreased equational segregation in the sgo1∆ rec12∆ background or the haploid sgo1∆ background. ------- COMMENT: 4899d62746d85c40 3 E/9CzKkmdXuhgo3vZOEygxC+/kk Figure 1. Increased equational segregation in the sgo1∆ background. ------- COMMENT: 4899d62746d85c40 6 1zm3ia41eEcEgtZyXolTYrT5jeU Importantly, the dam1Δ mutation impaired disjunction of homologous chromosomes (figure 5a), as seen in mad2Δ and ark1-so mutants [25,52]. ------- COMMENT: 4899d62746d85c40 8 xBmq7poej0J3M0MbnCshsLGqU+E Furthermore, the dam1Δ mutation increased equational segregation of sister chromatids in rec12+ cells (figure 5b, left) but it decreased equational segregation in sgo1Δ rec12Δ or haploid meiotic sgo1Δ cells (figure 5b,c) ------- COMMENT: 4899d62746d85c40 9 1yT0UXaBuilmedbDKCoGHuKNT5Y Decreased equational segregation in the diploid sgo1∆ rec12∆ background or the haploid sgo1∆ background. ------- COMMENT: 4899d62746d85c40 11 BELROSXqjAbaXtD8XuZfyISX5+M Anaphase A chromosome movement is completely abolished and only anaphase B chromosome movement occurs. ------- COMMENT: 4899d62746d85c40 12 C8tM9JESrz+ry1xgWzzIOO6pnyQ Figure 1. Increased equational segregation in sgo1∆ background. ------- COMMENT: 4899d62746d85c40 13 C8tM9JESrz+ry1xgWzzIOO6pnyQ Figure 1. Increased equational segregation in sgo1∆ background. ------- COMMENT: 4899d62746d85c40 14 Txakdv8vPQTfMdzYZh4OXxhoPME ***** increased bi-oriented attachment of sister chromatids in meiosis I*****The frequencies of sister centromere splitting varied (electronic supplementary material, figure S2A), but the mean splitting frequencies per centromere obtained by the bootstrap method were signifi- cantly higher in rec12Δ cells than in rec12+ cells (the difference was also significant in the usual t-test, p < 0.01; figure 2d, +). The elevated frequency of sister centromere splitting in chiasma-lacking cells confirms that chiasmata prevent bi-oriented attachment of sister chromatids. ------- COMMENT: 4899d62746d85c40 15 qlmBi8i//An6R/ahkY/CPfD1YeU The elevated frequency of sister centromere splitting in chiasma-lacking cells confirms that chiasmata prevent bi-oriented attachment of sister chromatids. ------- COMMENT: 48a191da3163b589 1 jSbKZY+KE8LeHeSktrjVIOulppI Php4 degraded in stationary phase (Fig. 1A) ------- COMMENT: 48a191da3163b589 2 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 48a191da3163b589 5 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 48a191da3163b589 6 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 48a191da3163b589 7 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 48a191da3163b589 8 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: 48a191da3163b589 9 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: 48a191da3163b589 10 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: 48a191da3163b589 11 IqGg16ioXLK9CShcgNqRHfsLHdc (Fig. 3A and 3E) ------- COMMENT: 48a191da3163b589 13 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 48a191da3163b589 14 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: 48a191da3163b589 16 DnQNbBCyjr9h1gZ1Ef6ybZH1kkk (Fig. 3F) ------- COMMENT: 48a191da3163b589 17 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 48a191da3163b589 18 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 48a191da3163b589 19 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 48a191da3163b589 20 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 48a191da3163b589 21 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 48a191da3163b589 22 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 48a191da3163b589 23 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 48a191da3163b589 24 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 48a191da3163b589 25 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 48a191da3163b589 26 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 48a191da3163b589 27 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 48a191da3163b589 28 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 48a191da3163b589 29 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 48a191da3163b589 30 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 48a191da3163b589 31 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 48a191da3163b589 32 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 48a191da3163b589 33 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 48a191da3163b589 34 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 48a191da3163b589 35 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 48a191da3163b589 36 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 48a191da3163b589 37 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 48a191da3163b589 38 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 48a191da3163b589 41 fxaiHEX4NLU0AhYbTTl1zelbfQQ These results suggest that Hul6 loosely associates with the outer mitochondrial membrane. (Fig. 6) ------- COMMENT: 48a191da3163b589 42 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: 48a191da3163b589 43 kb7NOBRiqlmzY3vQrPFeR5NkglI (Fig. 7C) ------- COMMENT: 48a191da3163b589 44 vGtIAlSSPNnMrmPTCV9WpCBeLHE (Fig. 7B) ------- COMMENT: 48a191da3163b589 45 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: 48a191da3163b589 46 p3x2dDTjQlS9jbO+IMHpHbED4nw (Fig. 1D) ------- COMMENT: 48a191da3163b589 47 0ioefkFyvW/WmkzwNc9/2j/m270 (Fig. S5B) ------- COMMENT: 48a191da3163b589 48 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: 48a191da3163b589 49 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: 48a191da3163b589 50 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 48a191da3163b589 51 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 48a191da3163b589 52 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 48a191da3163b589 54 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 48a191da3163b589 55 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 48a191da3163b589 56 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 48a191da3163b589 57 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 48a191da3163b589 58 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 48a191da3163b589 59 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 48a191da3163b589 60 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 48a191da3163b589 61 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 48a191da3163b589 62 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 48a191da3163b589 63 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 48a191da3163b589 64 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 48a191da3163b589 65 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 48a191da3163b589 66 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 48a191da3163b589 67 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 48a191da3163b589 68 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 48a191da3163b589 69 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 48a191da3163b589 70 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 48a191da3163b589 71 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 48a191da3163b589 72 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 48a191da3163b589 73 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 48a191da3163b589 74 RW+c44BwxNcMsCMMlYLXSmqj+AA (Fig. 4A and Fig. S6B) ------- COMMENT: 48a191da3163b589 75 RW+c44BwxNcMsCMMlYLXSmqj+AA (Fig. 4A and Fig. S6B) ------- COMMENT: 48a191da3163b589 76 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 48a191da3163b589 77 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 48a191da3163b589 78 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 48a191da3163b589 79 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 48a191da3163b589 80 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 48a191da3163b589 81 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 48a191da3163b589 82 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 48a191da3163b589 83 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 48a191da3163b589 84 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 48a191da3163b589 85 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 48a191da3163b589 86 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 48a191da3163b589 87 Ubtv11Qa4nxJ7BvWqW8dSQLoW6I Δhul6 cells show a progressive loss of viability, starting at 72 h (Fig. S6B). ------- COMMENT: 48a191da3163b589 88 1YIcte8fZLc7DHg1PlnYtmI12nk (Fig. 5B and 5C) ------- COMMENT: 48a191da3163b589 89 1YIcte8fZLc7DHg1PlnYtmI12nk (Fig. 5B and 5C) ------- COMMENT: 48a191da3163b589 90 1YIcte8fZLc7DHg1PlnYtmI12nk (Fig. 5B and 5C) ------- COMMENT: 48abddffaf7fd1e5 24 lxdw5xvq6sUWJT9FvLNDQUDZXf4 (comment: localization requires F-actin -assayed using latrunculin A) ------- COMMENT: 48abddffaf7fd1e5 25 lxdw5xvq6sUWJT9FvLNDQUDZXf4 (comment: localization requires F-actin -assayed using latrunculin A) ------- COMMENT: 48ad18999497e29d 2 tueprdhyYUziiaJY3qK0j7xdYc0 While viable, pfal1Δ showed a strong growth defect at all three temperatures tested (Fig. 1A). ------- COMMENT: 48ad18999497e29d 3 tueprdhyYUziiaJY3qK0j7xdYc0 While viable, pfal1Δ showed a strong growth defect at all three temperatures tested (Fig. 1A). ------- COMMENT: 48ad18999497e29d 4 tueprdhyYUziiaJY3qK0j7xdYc0 While viable, pfal1Δ showed a strong growth defect at all three temperatures tested (Fig. 1A). ------- COMMENT: 48ad18999497e29d 5 6SbY7OUIT91kfuyTe8kVCUfIgxI S. pombe, pFal1 localizes to chromatin-contain- ing regions of the nucleus and is not restricted to the nucleolus. ------- COMMENT: 48ad18999497e29d 6 6SbY7OUIT91kfuyTe8kVCUfIgxI S. pombe, pFal1 localizes to chromatin-contain- ing regions of the nucleus and is not restricted to the nucleolus. ------- COMMENT: 48ad18999497e29d 7 LxwvQODexDdGEbNuPzWl7zBSOi4 We chose to study mei4 + and ssm4 + transcripts because they both contain a DSR region (.........we observed an increase in mei4 + and ssm4 + transcripts during vegetative growth in cells containing red5-2 (Fig. 1D). ------- COMMENT: 48ad18999497e29d 8 LxwvQODexDdGEbNuPzWl7zBSOi4 We chose to study mei4 + and ssm4 + transcripts because they both contain a DSR region (.........we observed an increase in mei4 + and ssm4 + transcripts during vegetative growth in cells containing red5-2 (Fig. 1D). ------- COMMENT: 48ad18999497e29d 9 LxwvQODexDdGEbNuPzWl7zBSOi4 We chose to study mei4 + and ssm4 + transcripts because they both contain a DSR region (.........we observed an increase in mei4 + and ssm4 + transcripts during vegetative growth in cells containing red5-2 (Fig. 1D). ------- COMMENT: 48ad18999497e29d 10 ABp+isoqU39+Kf6usbWJcNsv+II Diploid pfal1Δ−/− cells show a decreased sporulation efficien- cy compared to wild-type cells (Fig. 2B). Less than 5% of pfal1Δ−/− cells sporulated, and ∼20% form misshapen asci on SPA medium (Fig. 2C), suggesting a meiotic defect. ------- COMMENT: 48ad18999497e29d 11 PH1OwTTtk/KLwVKWSOj5+Sf65ro Diploid red5-2 cells show severe sporulation defects, with <1% of cells producing asci. ------- COMMENT: 48ad18999497e29d 12 POeb4gu/HcEHK16G3qSqhF6FTZw qRT-PCR results show a large increase in rec8+ transcript levels during meiosis in wild-type cells, but no increase in pfal1Δ and red5-2 mutants (Fig. 3A). ------- COMMENT: 48ad18999497e29d 13 POeb4gu/HcEHK16G3qSqhF6FTZw qRT-PCR results show a large increase in rec8+ transcript levels during meiosis in wild-type cells, but no increase in pfal1Δ and red5-2 mutants (Fig. 3A). ------- COMMENT: 48ad18999497e29d 14 LxwvQODexDdGEbNuPzWl7zBSOi4 We chose to study mei4 + and ssm4 + transcripts because they both contain a DSR region (.........we observed an increase in mei4 + and ssm4 + transcripts during vegetative growth in cells containing red5-2 (Fig. 1D). ------- COMMENT: 48ad18999497e29d 15 eVPnd+J54wYAuUiHYmuO+GY6uAk These results demon- strate that pFal1, Red5, and Red1 are required to generate spliced rec8+ mRNA during starvation-induced meiosis. ------- COMMENT: 48ad18999497e29d 16 eVPnd+J54wYAuUiHYmuO+GY6uAk These results demon- strate that pFal1, Red5, and Red1 are required to generate spliced rec8+ mRNA during starvation-induced meiosis. ------- COMMENT: 48ad18999497e29d 17 eVPnd+J54wYAuUiHYmuO+GY6uAk These results demon- strate that pFal1, Red5, and Red1 are required to generate spliced rec8+ mRNA during starvation-induced meiosis. ------- COMMENT: 48ad18999497e29d 18 sI7J0wS6vTdwg7BAi7Lv2klPmIs Consistent with the meiotic defects, this shift did not occur efficiently in diploid homozygous pfal1 and red1 mutants, reflected by a decreased splicing index (Fig. 3). ------- COMMENT: 48ad18999497e29d 19 sI7J0wS6vTdwg7BAi7Lv2klPmIs Consistent with the meiotic defects, this shift did not occur efficiently in diploid homozygous pfal1 and red1 mutants, reflected by a decreased splicing index (Fig. 3). ------- COMMENT: 48ad18999497e29d 20 sI7J0wS6vTdwg7BAi7Lv2klPmIs Consistent with the meiotic defects, this shift did not occur efficiently in diploid homozygous pfal1 and red1 mutants, reflected by a decreased splicing index (Fig. 3). ------- COMMENT: 48ad18999497e29d 21 4sAq+Vr//mHF7yRWDgtChlV3mQ0 We can easily detect interaction between pFal1- Myc and Mnh1-FTP (Fig. 4A). ------- COMMENT: 48ad18999497e29d 22 4sAq+Vr//mHF7yRWDgtChlV3mQ0 We can easily detect interaction between pFal1- Myc and Mnh1-FTP (Fig. 4A). ------- COMMENT: 48ad18999497e29d 23 x31TkZRpHmlQPZzUyu1WeFtGmjY We also observed interactions of pFal1-Myc with Rnps1-GFP and Y14-HA (Fig. 4B,D). ------- COMMENT: 48ad18999497e29d 24 x31TkZRpHmlQPZzUyu1WeFtGmjY We also observed interactions of pFal1-Myc with Rnps1-GFP and Y14-HA (Fig. 4B,D). ------- COMMENT: 48ad18999497e29d 25 iYpsEyHi0mCBnh9ALeYsJtvz+c8 loss of mnh1 causes severe sporulation defects (Fig. 4F), ------- COMMENT: 48ad18999497e29d 26 VfvBD57zQ5k3mXZN+RzY0ryYh/0 Using qRT- PCR, we found a reduction in spliced meiotic transcripts of rec8+ in mnhΔ1−/− but not y14Δ−/− or rnps1Δ−/− (Fig. 5A), ------- COMMENT: 48ad18999497e29d 27 eVPnd+J54wYAuUiHYmuO+GY6uAk These results demon- strate that pFal1, Red5, and Red1 are required to generate spliced rec8+ mRNA during starvation-induced meiosis. ------- COMMENT: 48b52c7ce19007bd 1 +UMEfHcAvO2ioj+DVxUioxCvqgA Fig. 1. An atf1 deletion strain carrying the ade6 reporter gene inserted at the mat3-M locus displayed a loss of silencing of the reporter gene (Fig. 1A). ------- COMMENT: 48b52c7ce19007bd 2 b2yhMrLoPsgt/c+NWHDCnD/g9OQ Fig. 1A In contrast, the atf1 deletion resulted in increased transcriptional repression at both centromeres and telomeres (Fig. 1A). ------- COMMENT: 48b52c7ce19007bd 3 b2yhMrLoPsgt/c+NWHDCnD/g9OQ Fig. 1A In contrast, the atf1 deletion resulted in increased transcriptional repression at both centromeres and telomeres (Fig. 1A). ------- COMMENT: 48b52c7ce19007bd 4 UBN04z9yH1o5nKrF1CYTOVFZoXQ Fig. 1 Similar to atf1 mutants, pcr1 showed reduced silencing, but the effect was weaker than that of atf1 or atf1pcr1 (Fig. 1B). ------- COMMENT: 48b52c7ce19007bd 5 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 48b52c7ce19007bd 6 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 48b52c7ce19007bd 7 u2vZN97IcUGlWNKd55OgB1bEblg Fig. 3 Moreover, the ade6-off to ade6-on conversion in sty1 and wis1 mutants was significantly reduced relative to wild- type cells, indicating that sty1 and wis1 deletions enhanced stabilization of the epigenetic inheritance of the silent states (Fig. 3B). ------- COMMENT: 48b52c7ce19007bd 8 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 48b52c7ce19007bd 9 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 48b52c7ce19007bd 10 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 48b52c7ce19007bd 11 R94RKas1H5JKAQ7h9rS94ssGlGM Fig. 2B Furthermore, ChIP analysis showed that the absence of atf1 and pcr1 resulted in a considerable increase in histone H3/H4 acetylation and euchromatic-specific H3 Lys-4 methylation of the selected region ------- COMMENT: 48b52c7ce19007bd 12 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 48b52c7ce19007bd 13 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 48b52c7ce19007bd 14 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 48b52c7ce19007bd 15 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 48b52c7ce19007bd 16 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 48b52c7ce19007bd 17 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 48b52c7ce19007bd 18 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 48b52c7ce19007bd 19 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 48b52c7ce19007bd 20 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 48b52c7ce19007bd 21 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 48b52c7ce19007bd 22 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 48b52c7ce19007bd 23 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 48b52c7ce19007bd 24 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 48b52c7ce19007bd 25 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 48b52c7ce19007bd 26 unLU+saYvPSOMn2ELQRw/s+fcXY (comment: RNAi-independent mechanism) ------- COMMENT: 48b52c7ce19007bd 27 unLU+saYvPSOMn2ELQRw/s+fcXY (comment: RNAi-independent mechanism) ------- COMMENT: 48b52c7ce19007bd 31 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 48b52c7ce19007bd 32 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 48b52c7ce19007bd 33 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 48b52c7ce19007bd 34 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 48b52c7ce19007bd 35 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 48b52c7ce19007bd 36 VHqeb8f5rXwljB0Yt47LBu2SOhA (comment: ATF/CREB-binding) ------- COMMENT: 48b52c7ce19007bd 37 VHqeb8f5rXwljB0Yt47LBu2SOhA (comment: ATF/CREB-binding) ------- COMMENT: 48b52c7ce19007bd 38 Ni+cjEgf2igkXfo5+HE0lrY4MhI The results presented above suggest that the kinase activity of Wis1 and Sty1/Spc1 is required for proper control of hetero chromatin assembly by Atf1 and Pcr1. ------- COMMENT: 48b52c7ce19007bd 39 Ni+cjEgf2igkXfo5+HE0lrY4MhI The results presented above suggest that the kinase activity of Wis1 and Sty1/Spc1 is required for proper control of hetero chromatin assembly by Atf1 and Pcr1. ------- COMMENT: 48b52c7ce19007bd 40 unLU+saYvPSOMn2ELQRw/s+fcXY (comment: RNAi-independent mechanism) ------- COMMENT: 48d935f12b87d5c6 2 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 3 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 4 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 5 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 6 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 7 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 8 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 9 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 10 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 11 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 12 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 13 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 14 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 15 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 16 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 17 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 48d935f12b87d5c6 18 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 48d935f12b87d5c6 22 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 48d935f12b87d5c6 23 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 48d935f12b87d5c6 24 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 48d935f12b87d5c6 25 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 48d935f12b87d5c6 26 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: 48d935f12b87d5c6 27 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 48d935f12b87d5c6 28 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 48d935f12b87d5c6 29 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 48d935f12b87d5c6 30 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 48d935f12b87d5c6 31 2qg3cMr+v17DSrkc6oiD/9I/jyY Fig. 3F,G (comment: CHECK into G2) ------- COMMENT: 48d935f12b87d5c6 32 YYeCndfq7z48bh290m7DrkdMSRI (comment: CHECK *********** Fig. 3G decreased protein localization to spindle pole body during G1/S) ------- COMMENT: 48d935f12b87d5c6 33 Clt45H+nxo88Lhc5g7Ly1Iwu/4U Fig. 3H–J (comment:CHECK into G2) ------- COMMENT: 48d935f12b87d5c6 34 fCEKejVdyhh5rBbnAJXmHLJ2kmA Fig. 3I ------- COMMENT: 48d935f12b87d5c6 35 W8whJJDjMaw3TALlI9KS4zxBruI (comment: CEHCK ******** Fig. 3I decreased protein localization to spindle pole body during G1/S) ------- COMMENT: 48d935f12b87d5c6 36 8CHJjamUtTMf4hG4PaPdELq5LiI Fig. 3A,B ------- COMMENT: 48d935f12b87d5c6 37 8CHJjamUtTMf4hG4PaPdELq5LiI Fig. 3A,B ------- COMMENT: 48d935f12b87d5c6 38 Zj2M8+JcEyA+3M0Wz0RofAUhjlo Fig. 4E–H ------- COMMENT: 48d935f12b87d5c6 39 ki+tGuAQoXMziZriA28gSnZBCls Fig. 6B–I ------- COMMENT: 48d935f12b87d5c6 40 lwHQX3yr9Xznxpn4ujcnqiYJCu8 Fig. 7C,D ------- COMMENT: 48d935f12b87d5c6 41 4ovMQFYrhXi2RqqCYcCs5yQ/cRM Fig. 7C–E ------- COMMENT: 48d935f12b87d5c6 42 CVkVKvsXHG8UAbnXos1ukcCDxw0 Fig. 7G ------- COMMENT: 48d935f12b87d5c6 43 HLNIouxld6K2K83WDwF0Fvg1XBc Extended Data Fig. 3C ------- COMMENT: 48d935f12b87d5c6 44 y9xRiedFNLdWA7xXlykqUxQ3RiY Extended Data Fig. B,C ------- COMMENT: 48d935f12b87d5c6 45 TTKoEiwh00UxD4itM8aI6DkHKhk Extended Data Fig. 5B,C ------- COMMENT: 48d935f12b87d5c6 46 rCm19Z8qFOeQyCmar+Q5M/IKQ8g Extended Data Fig. 5E,F ------- COMMENT: 48d935f12b87d5c6 47 /OC2AmxPe3PgtAqDcrqAUZW22AI Extended Data Fig. 5E,F ------- COMMENT: 48d935f12b87d5c6 48 wguMemZvgPJ0bDRptVJNdJSo99E Extended Data Fig. 5G ------- COMMENT: 48d935f12b87d5c6 49 JgG1K8k3hVjZ3cvKLgvFN9t/cjw Extended Data Fig. 5I ------- COMMENT: 48d935f12b87d5c6 50 7ueqbJPfvRszxJl68D3mJSXuNk8 Extended Data Fig. 5H ------- COMMENT: 48d935f12b87d5c6 51 wguMemZvgPJ0bDRptVJNdJSo99E Extended Data Fig. 5G ------- COMMENT: 48d935f12b87d5c6 52 wguMemZvgPJ0bDRptVJNdJSo99E Extended Data Fig. 5G ------- COMMENT: 48d935f12b87d5c6 53 g/Gl1ECYT/CQMC85OKNBT4+tUog Extended Data Fig. 5H ------- COMMENT: 48d935f12b87d5c6 54 g/Gl1ECYT/CQMC85OKNBT4+tUog Extended Data Fig. 5H ------- COMMENT: 48d935f12b87d5c6 55 rmoV4u0DSqsVM5xCT9Q/zko2Rf8 Fig. 1B–D ------- COMMENT: 48d935f12b87d5c6 56 rmoV4u0DSqsVM5xCT9Q/zko2Rf8 Fig. 1B–D ------- COMMENT: 48d935f12b87d5c6 57 rmoV4u0DSqsVM5xCT9Q/zko2Rf8 Fig. 1B–D ------- COMMENT: 48d935f12b87d5c6 58 rmoV4u0DSqsVM5xCT9Q/zko2Rf8 Fig. 1B–D ------- COMMENT: 48d935f12b87d5c6 59 rmoV4u0DSqsVM5xCT9Q/zko2Rf8 Fig. 1B–D ------- COMMENT: 48d935f12b87d5c6 60 rmoV4u0DSqsVM5xCT9Q/zko2Rf8 Fig. 1B–D ------- COMMENT: 48d935f12b87d5c6 61 rmoV4u0DSqsVM5xCT9Q/zko2Rf8 Fig. 1B–D ------- COMMENT: 48d935f12b87d5c6 62 rmoV4u0DSqsVM5xCT9Q/zko2Rf8 Fig. 1B–D ------- COMMENT: 48d935f12b87d5c6 63 ksJUztJKm4oiimM+VslSakOMYFE Extended Data Fig. 1A,B ------- COMMENT: 48d935f12b87d5c6 64 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 48d935f12b87d5c6 65 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 48d935f12b87d5c6 69 IgMaceIZ231d1S3E/q+kBcsqFpo Fig. 4H ------- COMMENT: 48d935f12b87d5c6 70 qXZ98TvCRnpmoj0X58tGBxM4Jjk Fig. 5A–C ------- COMMENT: 48d935f12b87d5c6 71 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 48d935f12b87d5c6 72 FEMjT+tgWVLckWv4/mgD7Wv3Kxw (comment: 16 ± 8 molecules at the spindle pole body) Fig. 2 ------- COMMENT: 48d935f12b87d5c6 73 hxtSktS9ymx3Rgsw7FW+P+CCm70 (comment: 15 ± 8 molecules at the spindle pole body) Fig. 2 ------- COMMENT: 48d935f12b87d5c6 74 PQGVbSqYRurPskzRbhT0IVafe2Q (comment: 75 ± 45 molecules at the spindle pole body) Fig. 2 ------- COMMENT: 48d935f12b87d5c6 75 Hej4CV3THdtNaBYxyGYv6HkOezo (comment: 69 ± 37 molecules at the spindle pole body) Fig. 2 ------- COMMENT: 48d935f12b87d5c6 76 zk+wZ0qDbSSl74ozBmmLO8al4iQ (comment: 25 ± 13 molecules at the spindle pole body) Fig. 2 ------- COMMENT: 48d935f12b87d5c6 77 7+otECFJufvGq+F4giKBogllxPw (comment: 30 ± 17 molecules at the spindle pole body) Fig. 2 ------- COMMENT: 48d935f12b87d5c6 78 6pBRhDZXyvIRqyEytSjz2lhYNzY (comment: 77 ± 51 molecules at the spindle pole body) Fig. 2 ------- COMMENT: 48d935f12b87d5c6 79 lH5IRSxqZezlJyvVJ1I7RDLoPns (comment: 25 ± 10 molecules at the spindle pole body) Fig. 2 ------- COMMENT: 48d935f12b87d5c6 81 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 82 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 83 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 84 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 85 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 86 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 87 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 88 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 89 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 90 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 91 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 92 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 93 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 94 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 95 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 96 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 97 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 98 OhAnv7zN+hnuqyCjxtCfgr3ZOFI Extended Data Fig. 4 ------- COMMENT: 48d935f12b87d5c6 99 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 48d935f12b87d5c6 100 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 48d935f12b87d5c6 101 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 48d935f12b87d5c6 102 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 490a9063fbc7021a 4 1UBjCeCiJ7mCOebDi/BRm+PurzY We found that, in imp1D cells, NPCs were detected at the MMD and the peripheral NPC component Nup60 was removed from this domain (Figure 4C); however, the removal of structural components such as Nup107 and the membrane nucleoporin Cut11 was not observed (Figures 4C, S3A, and S3B). ------- COMMENT: 490a9063fbc7021a 7 Gas7vBA/yknzMFYXm0HvNjlZkLA A failed mitotic nuclear division is a cell phenotype observed at the end of mitosis during the vegetative life cycle in which the nuclear division does not occur and the two DNA masses remain linked by the internuclear membrane bridge. This can result in the coalescence of both DNA masses into one nucleus. ------- COMMENT: 490a9063fbc7021a 9 Gas7vBA/yknzMFYXm0HvNjlZkLA A failed mitotic nuclear division is a cell phenotype observed at the end of mitosis during the vegetative life cycle in which the nuclear division does not occur and the two DNA masses remain linked by the internuclear membrane bridge. This can result in the coalescence of both DNA masses into one nucleus. ------- COMMENT: 490a9063fbc7021a 14 Ek9diGrzNbLcWjyvwavwGyc6R5k A failed mitotic nuclear envelope division is a cell phenotype observed at the end of mitosis during the vegetative life cycle in which the nuclear division does not occur and the two DNA masses remain linked by the internuclear membrane bridge. This can result in the coalescence of both DNA masses into one nucleus. We found that wild-type cells showed timed NE division in the absence of actomyosin ring (Figures 4A and 4B), demon- strating that NE division is independent of cell division. However, 100% of imp1D cells (n = 126) completely failed to undergo NE division, resulting in cells with two nuclei linked by a long NE bridge (Figures 4A and 4B; Video S4). This result demonstrates that Imp1 is required for NE division. ------- COMMENT: 490a9063fbc7021a 16 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 490a9063fbc7021a 26 DgN/mi1YkHqezs0YWPvgZpRpYBM ase1D cells that elongated the spindle, the MMD was not properly formed, and the number of NPCs was variable (Figure S2B ------- COMMENT: 490a9063fbc7021a 27 DgN/mi1YkHqezs0YWPvgZpRpYBM ase1D cells that elongated the spindle, the MMD was not properly formed, and the number of NPCs was variable (Figure S2B ------- COMMENT: 490a9063fbc7021a 28 hbWmQ3v39ht4qSpiZe38YJ6GEdY Consistently, these cells showed a higher fre- quency of asymmetric NE divisions (Figure S2C) ------- COMMENT: 490a9063fbc7021a 29 3EY2H8cJDlvfOpXBo0xpjgVY6iI (comment: CHECK causally upstreasm of?) ------- COMMENT: 490a9063fbc7021a 31 00UjpKQmD+k2gDxN6z+SvYroUX0 Apq12 localized in tubules con- nected to or in close proximity to the MMD and at spindle pole regions, mirroring ER tubules marked with Yop1-GFP, Rtn1- GFP, or the artificial ER luminal marker mCherry-ADEL ------- COMMENT: 490a9063fbc7021a 32 00UjpKQmD+k2gDxN6z+SvYroUX0 Apq12 localized in tubules con- nected to or in close proximity to the MMD and at spindle pole regions, mirroring ER tubules marked with Yop1-GFP, Rtn1- GFP, or the artificial ER luminal marker mCherry-ADEL ------- COMMENT: 490a9063fbc7021a 33 ZqxzuSnBhf+tvUYWSJXErocYReI The coalescence of daughter nuclei was also observed in apq12D cells that failed to undergo nuclear division (Figure 4F). ------- COMMENT: 490a9063fbc7021a 34 qVfXvrnhHtai3qXi1L0vs4REW7I When these cells were left in this con- dition, daughter nuclei began to move closer to each other until they finally merged into one single nucleus (Figure 4E). This phenotype can be promoted if spindles are forced to disas- semble by treating the cells with 30 mg/mL MBC, resulting in 35% (n = 34) of nuclear coalescence events. ------- COMMENT: 4927cddae28a3185 1 PxGYNeSxrV5PD+XotSQQNBxfnSo Figure 1a ------- COMMENT: 4927cddae28a3185 2 MB8HfFxX5AmOgwbeZDObRjaHiEY Figure 1b ------- COMMENT: 4927cddae28a3185 3 MB8HfFxX5AmOgwbeZDObRjaHiEY Figure 1b ------- COMMENT: 4927cddae28a3185 4 mxrWq1uiXPNAB8WneGCaDb3EtRc Figure 1g ------- COMMENT: 4927cddae28a3185 5 mxrWq1uiXPNAB8WneGCaDb3EtRc Figure 1g ------- COMMENT: 4927cddae28a3185 6 OrJvam907zeGraGzSyizyjLd4qU Figure 2f lanes 1, 2 ------- COMMENT: 4927cddae28a3185 7 OrJvam907zeGraGzSyizyjLd4qU Figure 2f lanes 1, 2 ------- COMMENT: 4927cddae28a3185 8 OrJvam907zeGraGzSyizyjLd4qU Figure 2f lanes 1, 2 ------- COMMENT: 4927cddae28a3185 9 ky/+0122BdU7xvxUw6twS2LfsEw Figure 3a ------- COMMENT: 4927cddae28a3185 10 ky/+0122BdU7xvxUw6twS2LfsEw Figure 3a ------- COMMENT: 4927cddae28a3185 11 q2tbT8vVqufxdok28QU7web7DdU Figure 3a. t (Table 1) indicating that inhibition of Sid2 or Fin1 delayed the timing of mitotic commitment until a new size threshold for division was me ------- COMMENT: 4927cddae28a3185 12 q2tbT8vVqufxdok28QU7web7DdU Figure 3a. t (Table 1) indicating that inhibition of Sid2 or Fin1 delayed the timing of mitotic commitment until a new size threshold for division was me ------- COMMENT: 4927cddae28a3185 13 YruIAhidj8cj244LLuO+Cyyrih0 (comment: CHECK mitotic commitment) ------- COMMENT: 4927cddae28a3185 14 th9ApaRqV9xY4nghC2XknMo+Lis (Figure 4b) confirming that each kinase promotes mitotic commitment. ------- COMMENT: 4927cddae28a3185 16 Q3fSc8QMJJ3lJ6tNcsTZTybcp9s Figure 4d ------- COMMENT: 4927cddae28a3185 17 Q3fSc8QMJJ3lJ6tNcsTZTybcp9s Figure 4d ------- COMMENT: 4927cddae28a3185 18 Q3fSc8QMJJ3lJ6tNcsTZTybcp9s Figure 4d ------- COMMENT: 4927cddae28a3185 19 Q3fSc8QMJJ3lJ6tNcsTZTybcp9s Figure 4d ------- COMMENT: 4927cddae28a3185 20 Q3fSc8QMJJ3lJ6tNcsTZTybcp9s Figure 4d ------- COMMENT: 4927cddae28a3185 21 Q3fSc8QMJJ3lJ6tNcsTZTybcp9s Figure 4d ------- COMMENT: 4927cddae28a3185 22 Q3fSc8QMJJ3lJ6tNcsTZTybcp9s Figure 4d ------- COMMENT: 4927cddae28a3185 23 Q3fSc8QMJJ3lJ6tNcsTZTybcp9s Figure 4d ------- COMMENT: 4927cddae28a3185 24 Q3fSc8QMJJ3lJ6tNcsTZTybcp9s Figure 4d ------- COMMENT: 4927cddae28a3185 25 Q3fSc8QMJJ3lJ6tNcsTZTybcp9s Figure 4d ------- COMMENT: 4927cddae28a3185 26 Q3fSc8QMJJ3lJ6tNcsTZTybcp9s Figure 4d ------- COMMENT: 4927cddae28a3185 27 cHryrbq04yUzVYcezbhSIpKG22o (comment: CHECK upstream of pom1) ------- COMMENT: 493636e956e2fa13 1 dhDbKaN1xLB5BQ9IlXseSocVRtc (Figure 2a) ------- COMMENT: 493636e956e2fa13 2 PgrC2rRPixh7/xEf2kj+Bz8SY8Y (Figure 2b) ------- COMMENT: 49463cfe74c11d1f 1 becTFsV8SgCPRMxYJwsJItNtT1M (comment: CHECK cdc9-50 is the original name for wee1-50 allele. It was changed in subsequent publications to wee1 because of its phenotype and there is now no cdc9 gene) ------- COMMENT: 49463cfe74c11d1f 2 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 49463cfe74c11d1f 3 9kZWlQlUfwCJa+rwbjMm9Bocl9M Table 1, Figure 2 ------- COMMENT: 49463cfe74c11d1f 4 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 49463cfe74c11d1f 5 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 49463cfe74c11d1f 6 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 495506fbf084566b 3 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 495506fbf084566b 5 uN3TZTPWf9puluBOpnDXEiovh9s (comment: CHECK after chromosome segregation) ------- COMMENT: 495506fbf084566b 15 06sjqTsj3KH8yb80ZKd/Og3FRqA (comment: CHECK with extreme sister chromtid oscillations) ------- COMMENT: 495b692e25887eaf 34 9Oi4yHNQ7ebU1q9azp8IPejEEN0 ------- COMMENT: 495b692e25887eaf 36 4s5f6h8k7T061Vcsrt1MkcTXjrk (comment: CHECK permissive for cdc25-22; restrictive for cdt2-M1) ------- COMMENT: 49604a894be35067 1 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 49604a894be35067 2 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 49604a894be35067 3 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 49604a894be35067 4 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 49604a894be35067 5 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 49604a894be35067 6 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 49604a894be35067 7 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 49604a894be35067 8 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 49604a894be35067 9 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 49604a894be35067 10 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 49604a894be35067 11 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 49604a894be35067 12 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 49604a894be35067 13 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 49604a894be35067 14 y6t/BDey8TTHpWorQ7o1WCUfrgg Fig. S1 (comment: The authors define it as prolongued prophase-metaphase , but since they use the degradation as cdc13 as a marker, it really is anaphase onset that is measured.) ------- COMMENT: 49604a894be35067 15 y6t/BDey8TTHpWorQ7o1WCUfrgg Fig. S1 (comment: The authors define it as prolongued prophase-metaphase , but since they use the degradation as cdc13 as a marker, it really is anaphase onset that is measured.) ------- COMMENT: 49604a894be35067 16 y6t/BDey8TTHpWorQ7o1WCUfrgg Fig. S1 (comment: The authors define it as prolongued prophase-metaphase , but since they use the degradation as cdc13 as a marker, it really is anaphase onset that is measured.) ------- COMMENT: 49604a894be35067 17 y6t/BDey8TTHpWorQ7o1WCUfrgg Fig. S1 (comment: The authors define it as prolongued prophase-metaphase , but since they use the degradation as cdc13 as a marker, it really is anaphase onset that is measured.) ------- COMMENT: 49604a894be35067 18 Y1bN6ZqsCl1yOxWEJ7LFfNfrPWo Fig. S1 ((comment: CHECK This is a rescue of FYPO:0000324) ------- COMMENT: 49604a894be35067 19 Y1bN6ZqsCl1yOxWEJ7LFfNfrPWo Fig. S1 ((comment: CHECK This is a rescue of FYPO:0000324) ------- COMMENT: 49604a894be35067 20 sH010MLhLpGkSnJo/VAB/RHyJis Fig. S1 (comment: CHECK This is a partial rescue of FYPO:0004307) ------- COMMENT: 49604a894be35067 21 AcJCns+QkHlOqJpmuc/uZBWNWTQ (comment: CHECK This is a rescue of FYPO:0000324) ------- COMMENT: 49604a894be35067 22 AcJCns+QkHlOqJpmuc/uZBWNWTQ (comment: CHECK This is a rescue of FYPO:0000324) ------- COMMENT: 49604a894be35067 23 MEdY5JtEpUO97Kqlst/Mt6XjVjA (comment: CHECK This is a partial rescue of FYPO:0004307) ------- COMMENT: 49604a894be35067 24 dFJDzGnPY2TPrqb/uZh2zJT+NhQ Fig. 2 (comment: CHECK This is a rescue of FYPO:0004395) ------- COMMENT: 49604a894be35067 25 dFJDzGnPY2TPrqb/uZh2zJT+NhQ Fig. 2 (comment: CHECK This is a rescue of FYPO:0004395) ------- COMMENT: 49604a894be35067 28 DkgTu2N4JEbKcNy+gAV1ftJYMcE (comment: CHECK This is a rescue of FYPO:0004395 and FYPO:0004101 partially) ------- COMMENT: 49604a894be35067 29 1Ihu6pfiONqtjTVcp73jiEAkBS4 (comment: CHECK This is a rescue of FYPO:0004395) ------- COMMENT: 49604a894be35067 32 Ux6p5CqvlAYeiM+JTleYC2j/R8U Fig. 2 (comment: CHECK This is a rescue of FYPO:0000324) ------- COMMENT: 49604a894be35067 33 AcJCns+QkHlOqJpmuc/uZBWNWTQ (comment: CHECK This is a rescue of FYPO:0000324) ------- COMMENT: 49604a894be35067 37 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 49604a894be35067 38 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 49604a894be35067 43 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 49604a894be35067 44 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 49604a894be35067 45 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 49604a894be35067 46 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 49604a894be35067 47 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 496a21574b3d3a26 2 mwkDVfaNZDogm/jQklPtKSU8XeI fig 1 A ------- COMMENT: 496a21574b3d3a26 4 mwkDVfaNZDogm/jQklPtKSU8XeI fig 1 A ------- COMMENT: 496a21574b3d3a26 11 GC++j9yrmGrN29cDVzZsnaOCe40 Figures S1C and S1D ------- COMMENT: 496a21574b3d3a26 13 BiJvQ9QDCfvrgyLkNEO/nzxf7aU Figure S1C ------- COMMENT: 496a21574b3d3a26 14 Q0RkHJgJjR4UPXKqNU6MnAfV8ZI Figure 1B ------- COMMENT: 496a21574b3d3a26 15 Q0RkHJgJjR4UPXKqNU6MnAfV8ZI Figure 1B ------- COMMENT: 496a21574b3d3a26 16 Q0RkHJgJjR4UPXKqNU6MnAfV8ZI Figure 1B ------- COMMENT: 496a21574b3d3a26 18 Q0RkHJgJjR4UPXKqNU6MnAfV8ZI Figure 1B ------- COMMENT: 496a21574b3d3a26 20 t6wsS9jeQ8eVcEUQF8BCcAXmG6k Figure 1C ------- COMMENT: 496a21574b3d3a26 21 ViyrEX8oQ/W8/L3ac6o3yHEwvuY Figure 1C ------- COMMENT: 496a21574b3d3a26 22 t6wsS9jeQ8eVcEUQF8BCcAXmG6k Figure 1C ------- COMMENT: 496a21574b3d3a26 23 t6wsS9jeQ8eVcEUQF8BCcAXmG6k Figure 1C ------- COMMENT: 496a21574b3d3a26 24 ViyrEX8oQ/W8/L3ac6o3yHEwvuY Figure 1C ------- COMMENT: 496a21574b3d3a26 25 ViyrEX8oQ/W8/L3ac6o3yHEwvuY Figure 1C ------- COMMENT: 496a21574b3d3a26 26 k/1y/ouzXsX5OG6UXZD+bdmyQGM Figure S1E ------- COMMENT: 496a21574b3d3a26 27 k/1y/ouzXsX5OG6UXZD+bdmyQGM Figure S1E ------- COMMENT: 496a21574b3d3a26 28 k/1y/ouzXsX5OG6UXZD+bdmyQGM Figure S1E ------- COMMENT: 496a21574b3d3a26 29 k/1y/ouzXsX5OG6UXZD+bdmyQGM Figure S1E ------- COMMENT: 496a21574b3d3a26 30 k/1y/ouzXsX5OG6UXZD+bdmyQGM Figure S1E ------- COMMENT: 496a21574b3d3a26 31 k/1y/ouzXsX5OG6UXZD+bdmyQGM Figure S1E ------- COMMENT: 496a21574b3d3a26 32 k/1y/ouzXsX5OG6UXZD+bdmyQGM Figure S1E ------- COMMENT: 496a21574b3d3a26 33 k/1y/ouzXsX5OG6UXZD+bdmyQGM Figure S1E ------- COMMENT: 496a21574b3d3a26 34 mwkDVfaNZDogm/jQklPtKSU8XeI Figure 1A ------- COMMENT: 496a21574b3d3a26 37 mwkDVfaNZDogm/jQklPtKSU8XeI Figure 1A ------- COMMENT: 496a21574b3d3a26 38 mwkDVfaNZDogm/jQklPtKSU8XeI Figure 1A ------- COMMENT: 496a21574b3d3a26 43 aR3y1/htBisgf/GTM5jw8a8XvgA Figure S4 ------- COMMENT: 496a21574b3d3a26 50 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 496a21574b3d3a26 53 9QQ7HthEXp/JWeCIYT7IquPfxAo fig 4C ------- COMMENT: 496a21574b3d3a26 54 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 49c4f596e9b38eb7 1 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 49c4f596e9b38eb7 2 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 49c4f596e9b38eb7 3 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 49c4f596e9b38eb7 4 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 49c4f596e9b38eb7 7 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 49c4f596e9b38eb7 10 vxIYVbYY99/DILDNJRuzHoe8gdw Fig. 2D (comment: no tea3GFP staining cell middle in nda3 block) ------- COMMENT: 49c4f596e9b38eb7 11 dOgfzT6YDycy4rEivdQHJG7UaEY Fig. 2D (comment: tea3GFP staining cell middle after nda3 block and release) ------- COMMENT: 49c4f596e9b38eb7 14 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 49c4f596e9b38eb7 16 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 49c4f596e9b38eb7 17 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 49c4f596e9b38eb7 19 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 49c4f596e9b38eb7 20 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 49c4f596e9b38eb7 21 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 49c4f596e9b38eb7 22 OLFF1PB+6RkVNk94s/nuzIA3vdw Fig. 4D (comment: tea3 does not affect polarity and the elongated cells do not branch. This is different to pom1 where cdc11-119 cells form branches) ------- COMMENT: 49c4f596e9b38eb7 25 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 49c4f596e9b38eb7 26 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 49c4f596e9b38eb7 27 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 49c4f596e9b38eb7 28 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 49c4f596e9b38eb7 29 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 49c4f596e9b38eb7 32 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 49c4f596e9b38eb7 33 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 49fe99dfe092c9fa 5 OmDBLi6B9I6QoXBHPDfngHkj6l8 (comment: abolishes preference for K4-trimethylated H3) ------- COMMENT: 49fe99dfe092c9fa 6 K41a3zJLjOq4nGg7e78sg6Res90 (comment: acetyltransferase normally processive in presence of K4-trimethylated H3 -bound by Sgf29) ------- COMMENT: 49fe99dfe092c9fa 8 OmDBLi6B9I6QoXBHPDfngHkj6l8 (comment:abolishes preference for K4-trimethylated H3) ------- COMMENT: 49fe99dfe092c9fa 10 K41a3zJLjOq4nGg7e78sg6Res90 (comment: acetyltransferase normally processive in presence of K4-trimethylated H3 -bound by Sgf29) ------- COMMENT: 4a2182374ad8d34e 8 fK52EY9JxPESPUm7jNtF1MIRgb0 (comment: Cdc42-GTP assayed with CRIB- broad zones of activity) ------- COMMENT: 4a2182374ad8d34e 12 mrB+f4M7x/0m3f7KVRwPno0AEL0 (comment: CHECK wider cells) ------- COMMENT: 4a2182374ad8d34e 13 mrB+f4M7x/0m3f7KVRwPno0AEL0 (comment:CHECK wider cells) ------- COMMENT: 4a2182374ad8d34e 14 mrB+f4M7x/0m3f7KVRwPno0AEL0 (comment: CHECK wider cells) ------- COMMENT: 4a2182374ad8d34e 15 mrB+f4M7x/0m3f7KVRwPno0AEL0 (comment: CHECK wider cells) ------- COMMENT: 4a2182374ad8d34e 16 mrB+f4M7x/0m3f7KVRwPno0AEL0 (comment: CHECK wider cells) ------- COMMENT: 4a2182374ad8d34e 17 mrB+f4M7x/0m3f7KVRwPno0AEL0 (comment: CHECK wider cells) ------- COMMENT: 4a2275697a944b76 1 QqmgRLHMCoIgC++Afmch5pYKkY8 (comment: CHECK ****STATIONARY PhASE****) the protein level of Fzo1 is unstable during the stationary phase. ------- COMMENT: 4a2275697a944b76 4 26MXFyADk0z7f4W2jhDbP/0eVv8 the protein level of Fzo1 was not degraded in ∆rsv2 mutant in stationary phase. ------- COMMENT: 4a2275697a944b76 5 S+69mCvEl3dOcJVXdf8uiSs9RBk the protein level of Fzo1 was not degraded in Δubc8 mutant in stationary phase. ------- COMMENT: 4a2275697a944b76 16 SOppybwI8ILjNTNRBGYmM2Stx5M (Fig. 3). We found that Fzo1 protein was not degraded at late time points in the ∆rsv2 mutant ------- COMMENT: 4a2275697a944b76 17 S5nYdSHaaEg3D9+nxkyKYKugxvY (Fig. S1) The results showed that Fzo1 protein was not degraded at late time points only in the Δubc8 mutant (Fig. 4). Fzo1 protein was not degraded in ∆rsv2 and Δubc8 mutants after longer incubation times (60 and 72 h) ------- COMMENT: 4a2275697a944b76 19 cqAHxfaUq7BuLH2BRXSmQPL0MDk (Fig. 5) We found that when Fzo1 protein was overexpressed, it was no longer degraded at late time points ------- COMMENT: 4a2275697a944b76 20 V6r+wb5fRKALL26K7bJfa9kqKVk (comment: CHECK -v regulation, stationary phase) ------- COMMENT: 4a2275697a944b76 21 MEX7OvnCqS4JJH5ChyAqbmDPSRs (comment: -ve regulation stationary phase) ------- COMMENT: 4a2c0c67da8496f7 1 fFOYtoAic5XRUgPyw0itlUaNGPU Second, eRF1 and Dom34 increase the bind- ing of GTP to eRF3 and Hbs1, respectively, and GTP increases the binding of eRF1 and Dom34 to eRF3 and Hbs1, respectively28,32,33 (Fig. 6 and Supplementary Table 1). These interactions suggest that in both complexes the status of the nucleotide affects the interaction of the proteins and thereby modulates the function of the complex. ------- COMMENT: 4a2c0c67da8496f7 2 fFOYtoAic5XRUgPyw0itlUaNGPU Second, eRF1 and Dom34 increase the bind- ing of GTP to eRF3 and Hbs1, respectively, and GTP increases the binding of eRF1 and Dom34 to eRF3 and Hbs1, respectively28,32,33 (Fig. 6 and Supplementary Table 1). These interactions suggest that in both complexes the status of the nucleotide affects the interaction of the proteins and thereby modulates the function of the complex. ------- COMMENT: 4a65f559e9059c86 1 l0eNfiY2DeqhXxn16YXX8z99lrI The kin1-GFP strain exhibited a wild type phenotype. In living interphase cells, Kin1-GFP signal captured either in static images (Fig. 1A) or by time-lapse video microscopy (Fig. 1C) was localized at the cell tips (arrows, Fig. 1A). The signal appeared as dynamic dots close to the plasma membrane (Suppl. movie S1). Kin1-GFP was detected on the new cell end in early G2 as previously reported24 but also on the old end soon after growth resumption (arrow, Fig. 1C) conversely to the previous report.24 ------- COMMENT: 4a65f559e9059c86 2 3WdU5Bfi5ohZDxulghCa5Ivx7yg Kin1-GFP was detected on the new cell end in early G2 as previously reported24 but also on the old end soon after growth resumption (arrow, Fig. 1C) conversely to the previous report.24 ------- COMMENT: 4a65f559e9059c86 3 KCVdwRlYDZyY1TICgciEMj8ntvI Indeed, in mitotic cells, we detected a Kin1-GFP signal at the division site (arrowheads, Fig. 1A and C). Kin1-GFP appeared as a ring overlying the cell equator prior to nuclei separation at the onset of anaphase B (Fig. 1C). ------- COMMENT: 4a65f559e9059c86 4 mh55XKycPejiC2mBy36MBYV4iQg Kin1Δ cells synthesize normal septa (Fig. 2A) but exhibit a high septation index and a small percentage of multi- septate cells (Fig. 2B), indicating a cell separation defect as previously shown.22-24 ------- COMMENT: 4a65f559e9059c86 5 mh55XKycPejiC2mBy36MBYV4iQg Kin1Δ cells synthesize normal septa (Fig. 2A) but exhibit a high septation index and a small percentage of multi- septate cells (Fig. 2B), indicating a cell separation defect as previously shown.22-24 ------- COMMENT: 4a65f559e9059c86 6 mh55XKycPejiC2mBy36MBYV4iQg Kin1Δ cells synthesize normal septa (Fig. 2A) but exhibit a high septation index and a small percentage of multi- septate cells (Fig. 2B), indicating a cell separation defect as previously shown.22-24 ------- COMMENT: 4a65f559e9059c86 7 6cf0om3PQNj+M6Y7GQ1o29HHMBY Kin1Δ tea4Δ cells showed a severe cytokinetic phenotype (Fig. 2A), with aberrant septal material randomly depos- ited in a majority of cells (Fig. 2B). ------- COMMENT: 4a65f559e9059c86 9 wszhYcu0Qs5quzS2kbATyPxiI4o Moreover, double mutant cells completey lose their polarity and appeared almost round. This phenotype is strickingly similar to that of kin1Δ tea1Δ (Fig. 2A and B). ------- COMMENT: 4a65f559e9059c86 10 wszhYcu0Qs5quzS2kbATyPxiI4o Moreover, double mutant cells completey lose their polarity and appeared almost round. This phenotype is strickingly similar to that of kin1Δ tea1Δ (Fig. 2A and B). ------- COMMENT: 4a65f559e9059c86 11 wszhYcu0Qs5quzS2kbATyPxiI4o Moreover, double mutant cells completey lose their polarity and appeared almost round. This phenotype is strickingly similar to that of kin1Δ tea1Δ (Fig. 2A and B). ------- COMMENT: 4a65f559e9059c86 12 wszhYcu0Qs5quzS2kbATyPxiI4o Moreover, double mutant cells completey lose their polarity and appeared almost round. This phenotype is strickingly similar to that of kin1Δ tea1Δ (Fig. 2A and B). ------- COMMENT: 4a65f559e9059c86 13 wszhYcu0Qs5quzS2kbATyPxiI4o Moreover, double mutant cells completey lose their polarity and appeared almost round. This phenotype is strickingly similar to that of kin1Δ tea1Δ (Fig. 2A and B). ------- COMMENT: 4a65f559e9059c86 14 QC/zcuC4JnyysgdFy0B7iW2jiT8 During mitosis, the tip signal was detected on the most distant cell end relative to the asymmetric ring (arrow, Fig. 1B). Kin1-GFP was also detected at the division site in tea4Δ (arrowhead, Fig. 1B). We conclude that correct local- ization of Kin1-GFP does not require Tea4. ------- COMMENT: 4a65f559e9059c86 15 QC/zcuC4JnyysgdFy0B7iW2jiT8 During mitosis, the tip signal was detected on the most distant cell end relative to the asymmetric ring (arrow, Fig. 1B). Kin1-GFP was also detected at the division site in tea4Δ (arrowhead, Fig. 1B). We conclude that correct local- ization of Kin1-GFP does not require Tea4. ------- COMMENT: 4a65f559e9059c86 16 cNyIuxR76IepiHxwwngYh9e3vv0 In Kin1 downregulating cells, we observed that septa were orthogonal to the long cell axis but their positions seemed to be frequently eccentric (Kin1 OFF, Fig. 3A). ------- COMMENT: 4a65f559e9059c86 17 L/FgMixeRkfj2C1mCKXvs0ToaNs Cell shape or interphase F-actin polarity defects were not observed (Suppl. Fig. S1) conversely to the deletion strain. ------- COMMENT: 4a65f559e9059c86 18 j3dekzhEOoCUL1K+GT4BycjCYiE of the nucleus relative to the cell ends in late G2 cells (≥10 μm) and we observed that a higher proportion of nuclei were misplaced towards the new cell end ------- COMMENT: 4a65f559e9059c86 19 q4X4+BQJcOvBLx1AFOivv9kkNa4 Kin1 downregulation promoted aberrant septum material synthesis in tea4Δ: a main asymmetric septal structure was observed together with aberrant septal deposits along the cortex or stretched struc- tures that run along the main cell axis were detected (Fig. 4B) ------- COMMENT: 4a65f559e9059c86 20 Jle1CkjtFLbxmcKD8F12hv2JbeM Kin1 was downregulated. Upon Kin1 repression, we observed a defect in F-actin incorpora- tion into the CAR at mitosis compared to Kin1 ON. In 84% of anaphase cells, F-actin patches and/or cables were present outside ------- COMMENT: 4a65f559e9059c86 21 fF0zJzNasxWxtyZcl7iPd/XIEqU Thus, Kin1 downregulation in tea4Δ uncoupled packed ring formation and constriction but also altered ring disassembly in a subset of cells. ------- COMMENT: 4a65f559e9059c86 22 b+d6SUsvI5wQlLEFdopDKSfjJ0A Hence, Kin1-K154R acts as a dominant negative mutant and its primary effect is to inhibit maintenance of the nucleus at the geometric cell centre. ------- COMMENT: 4a65f559e9059c86 23 n5nzFmtSjPHG8BRexrO2Ftnzyno he par1Δ mutant shows a nuclear and septum (arrowhead, Fig. 6C; 25.6% of asymmetric septa versus 10.2% in wild type cells) ------- COMMENT: 4a75629ac3e4b424 5 UN2c4xHsBpRehfx+/yhH56Yzlrs (comment: CHECK based just on this paper, candidate for involved_in_or_regulates qualifier) ------- COMMENT: 4a75629ac3e4b424 9 UN2c4xHsBpRehfx+/yhH56Yzlrs (comment: CHECK based just on this paper, candidate for involved_in_or_regulates qualifier) ------- COMMENT: 4a75629ac3e4b424 27 AY5x6UAoQx+iC/aRYan3qcb4x8Q (comment: CHECK assayed in vitro) ------- COMMENT: 4a75629ac3e4b424 36 AY5x6UAoQx+iC/aRYan3qcb4x8Q (comment: CHECK assayed in vitro) ------- COMMENT: 4ac0ce427dd07a35 1 iX/x2LqXd1p4gBfphvyS5xnRXiw A serial dilution spotting assay (Fig. 2B) showed that the growth of och1Δ with either pAL-pwp1+ or pAU-pwp1+ was as fast as that of wild-type cells, indicating that the growth defect of och1Δ cells was alleviated by expression of pwp1+. ------- COMMENT: 4ac0ce427dd07a35 2 iX/x2LqXd1p4gBfphvyS5xnRXiw A serial dilution spotting assay (Fig. 2B) showed that the growth of och1Δ with either pAL-pwp1+ or pAU-pwp1+ was as fast as that of wild-type cells, indicating that the growth defect of och1Δ cells was alleviated by expression of pwp1+. ------- COMMENT: 4ac0ce427dd07a35 5 iX/x2LqXd1p4gBfphvyS5xnRXiw A serial dilution spotting assay (Fig. 2B) showed that the growth of och1Δ with either pAL-pwp1+ or pAU-pwp1+ was as fast as that of wild-type cells, indicating that the growth defect of och1Δ cells was alleviated by expression of pwp1+. ------- COMMENT: 4aeea6750a0a5a58 4 qM27kO6J5832L8Q1oFU4HoY4ycI In SGs after hyperosmotic shock (1 M KCl) but not after glucose deprivation ------- COMMENT: 4aff66ed2ed54ace 53 BuE9e+pzHiO++ZR0ye+j+7FFtxI implies that dna2 E560A alone is inviable ------- COMMENT: 4aff66ed2ed54ace 55 5Nov7erdb1Uh/VjhrT7eqdRRICc implies that dna2 K961T alone is viable ------- COMMENT: 4aff66ed2ed54ace 56 SBoHY5mbVFfq1ixDjTJ7LTM6LVE cleaves unpaired nascent DNA in replication forks ------- COMMENT: 4b104f39c2d5ae0c 1 Am0kjmUysRAWL/4jN3S5VzotyGw Figure 1a ------- COMMENT: 4b104f39c2d5ae0c 2 OBFd5QQGQsatgICaJMrqFN+R61Y Figure 1B ------- COMMENT: 4b104f39c2d5ae0c 3 Odj3D5uZfmI0/zRTUI+ge3n6T8s Figure 1B (comment: This phenotype is known as “VIC” viable in the presence of immunosup- pressant and chloride ion) ------- COMMENT: 4b104f39c2d5ae0c 8 o5vMN8yCEXCf3Qr1V9Vlpvwi1ls (comment: Cell wall damage induced with caspofungin) ------- COMMENT: 4b104f39c2d5ae0c 10 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 4b1bf8c741c5b2a3 11 eBtmtvyLM8Bx302IxS6faaUqfNU (comment: inferred from increased mutation rate upon UV exposure in mutant) ------- COMMENT: 4b1bf8c741c5b2a3 12 pv14VvgsRRxvWYOpja4E0vo6U3I (comment: rationale: increased transversion frequency indicates that 8-oxoG persists more in mutant, but normal indel frequency suggests not NER) ------- COMMENT: 4b35693cfa0373c0 13 YXn7u7UW4HgUIs7fwEsCLln52Ss (comment: same as rad50delta alone) ------- COMMENT: 4b35693cfa0373c0 15 aF2gHehr82AGU4AIemu/9AIv//g (comment: same as rad11-D223Y alone) ------- COMMENT: 4b35693cfa0373c0 16 aF2gHehr82AGU4AIemu/9AIv//g (comment: same as rad11-D223Y alone) ------- COMMENT: 4b91e1b64edf570f 1 gR+LJ54r2Ij/F27peglBW72dzio Through Rho1 activation, Rgf1 stabilizes Tea4 at the cell ends, promoting its accumulation. Additionally, we described an alternative actin-dependent mechanism, driven by Rgf1 and Rho1, for marking the poles independently to the known MT- and Tea-dependent pathway. ------- COMMENT: 4b91e1b64edf570f 5 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: 4b91e1b64edf570f 7 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 4b91e1b64edf570f 8 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: 4b91e1b64edf570f 9 9NARxUZ2eFayrFchKerC5tFVQ/4 (Fig. S5J) ------- COMMENT: 4b91e1b64edf570f 11 biv5vzYpNRqMrXY+5I3wXOO1GLA (Fig. 1G) ------- COMMENT: 4b91e1b64edf570f 12 xnWwtg3TqqhqbUHXva5kkxV96Hs (Fig. 1H) ------- COMMENT: 4b91e1b64edf570f 13 WD3kRd80SyyYyGSzDepPv0TldzQ (Fig. 3G) ------- COMMENT: 4b91e1b64edf570f 14 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: 4b91e1b64edf570f 15 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 4b91e1b64edf570f 16 DnQNbBCyjr9h1gZ1Ef6ybZH1kkk (Fig. 3F) ------- COMMENT: 4b91e1b64edf570f 17 d2NdxnatvYWNnh9afhcOiSOs0XA (Fig. S1D) ------- COMMENT: 4b91e1b64edf570f 18 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 4b91e1b64edf570f 20 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 4b91e1b64edf570f 21 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 4b91e1b64edf570f 23 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 4b91e1b64edf570f 24 MfhXrratyk9HEpPvYNKyPqJLEoI (Fig. 4E) ------- COMMENT: 4b91e1b64edf570f 25 MfhXrratyk9HEpPvYNKyPqJLEoI (Fig. 4E) ------- COMMENT: 4b91e1b64edf570f 26 e01sXaQ4tKZmhVThlh1qhf/lyQY (Fig. S4G) ------- COMMENT: 4b91e1b64edf570f 27 e01sXaQ4tKZmhVThlh1qhf/lyQY (Fig. S4G) ------- COMMENT: 4b91e1b64edf570f 28 HQx/nKU+Np0MQgZtcp2gru69Zq0 (Fig. S4E) ------- COMMENT: 4b91e1b64edf570f 29 j30KwK8QbZkLhoky9eRwFbLzgAY (Fig. S4C) ------- COMMENT: 4b91e1b64edf570f 30 1vITJkX365/kSO7rGYqR9cXiOZI (Fig. S4D) ------- COMMENT: 4b91e1b64edf570f 31 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: 4b91e1b64edf570f 37 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: 4b91e1b64edf570f 38 q2ifWLUMPt99XzRAlld7KR3J1LY (Fig. S5C) ------- COMMENT: 4b91e1b64edf570f 41 HuytyxXo/S0d9L/1IkHzY5Kgi+U (Fig. S5G) ------- COMMENT: 4b91e1b64edf570f 42 9BqZcwjB8FqFBdLSdXWg8a+RyNQ (Fig. S5H) ------- COMMENT: 4b91e1b64edf570f 43 9NARxUZ2eFayrFchKerC5tFVQ/4 (Fig. S5J) ------- COMMENT: 4b91e1b64edf570f 44 uytlu1SOwRPui227BsVN/IEzOy4 (Fig. S2A) ------- COMMENT: 4b91e1b64edf570f 45 gmqogAnY3BQANKEbZmiyaveRfrg (Fig. 3E) ------- COMMENT: 4b91e1b64edf570f 46 Plk5nf0Lc0Zs1bJbFO/+XPs3+dM (Fig. 1A, B and F) ------- COMMENT: 4b91e1b64edf570f 47 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: 4b91e1b64edf570f 48 xnWwtg3TqqhqbUHXva5kkxV96Hs (Fig. 1H) ------- COMMENT: 4b91e1b64edf570f 49 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: 4b91e1b64edf570f 50 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 4b91e1b64edf570f 51 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 4b91e1b64edf570f 52 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 4b91e1b64edf570f 53 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 4b91e1b64edf570f 54 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 4b91e1b64edf570f 55 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 4b91e1b64edf570f 56 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 4b91e1b64edf570f 57 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: 4b91e1b64edf570f 58 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: 4b91e1b64edf570f 59 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 4b91e1b64edf570f 60 gmqogAnY3BQANKEbZmiyaveRfrg (Fig. 3E) ------- COMMENT: 4b91e1b64edf570f 61 dJB6QAubOSayNATA9sbJUiUH0oE (Fig. 3H) ------- COMMENT: 4b91e1b64edf570f 62 dJB6QAubOSayNATA9sbJUiUH0oE (Fig. 3H) ------- COMMENT: 4b91e1b64edf570f 63 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 4b91e1b64edf570f 64 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 4b91e1b64edf570f 65 GkI93EYSqP6NybAtsJjxO2fy3Ic (Fig. 4D) ------- COMMENT: 4b91e1b64edf570f 66 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: 4b91e1b64edf570f 67 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: 4b91e1b64edf570f 68 DVJMM6AvJyLz8J8dkwtY+pFizxc (Fig. 5E) ------- COMMENT: 4b91e1b64edf570f 69 DVJMM6AvJyLz8J8dkwtY+pFizxc (Fig. 5E) ------- COMMENT: 4b91e1b64edf570f 70 DVJMM6AvJyLz8J8dkwtY+pFizxc (Fig. 5E) ------- COMMENT: 4b91e1b64edf570f 72 cy+Qtfso/xGGh5Pl16yZlXRX1ZQ (Fig. S5D) ------- COMMENT: 4b91e1b64edf570f 74 0ioefkFyvW/WmkzwNc9/2j/m270 (Fig. S5B) ------- COMMENT: 4b91e1b64edf570f 75 0ioefkFyvW/WmkzwNc9/2j/m270 (Fig. S5B) ------- COMMENT: 4b91e1b64edf570f 76 0ioefkFyvW/WmkzwNc9/2j/m270 (Fig. S5B) ------- COMMENT: 4bb49dd30b805a0c 1 84z0KR2LciI6d1y1bOzM/DrNF9o Clr4 deletion shows synthetic growth defect on FOA containing media ------- COMMENT: 4bb49dd30b805a0c 3 hRMhJZ7bPpe3piU7q8lyMDD38Zs found in nucleolus, chromatin ------- COMMENT: 4bb49dd30b805a0c 4 hsnXtN2JUG4rgqLV+HYL6XjdFNc Binds at H3K9 methylation site and helps in recruitment of various regulators at heterochromatin ------- COMMENT: 4bb49dd30b805a0c 5 ajhKe7tQKxfieTMvzyyFXhXRwSQ RNA binding protein and helps in recruitment of CLR complex at the heterochromatin regions ------- COMMENT: 4bb49dd30b805a0c 6 2qyt49FjThAWet4RGIcYgy2U4HE Helps in heterochromatin maintenance and stability as vgl1 deletion prevents H3K9 methylation and swi6 recruitment to centromeric and telomeric heterochromatin and prevents heterochromatin mediated gene silencing in S.Pombe ------- COMMENT: 4bb49dd30b805a0c 7 sHh3yAIAL1D5HpeJj+W2xk2TdTk Found in both nucleus and cytoplasm ------- COMMENT: 4bc8f8330173e8e4 1 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 4bc8f8330173e8e4 2 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 4bc8f8330173e8e4 3 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 4bc8f8330173e8e4 4 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 4bc8f8330173e8e4 5 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 4bc8f8330173e8e4 6 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 4bc8f8330173e8e4 7 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 4bc8f8330173e8e4 8 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 4bc8f8330173e8e4 9 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 4bc8f8330173e8e4 10 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 4bc8f8330173e8e4 11 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 4bc8f8330173e8e4 12 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 4bc8f8330173e8e4 13 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 4bc8f8330173e8e4 14 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 4bc8f8330173e8e4 15 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 4bc8f8330173e8e4 16 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 4bc8f8330173e8e4 17 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 4bd2526203a7cc33 1 sN9oTuN7rp2vzh7feqryMQTbE5U Fig 4B; (comment: CONDITION 0.5 mM H2O2 in agar) ------- COMMENT: 4bd2526203a7cc33 2 sN9oTuN7rp2vzh7feqryMQTbE5U Fig 4B; (comment: CONDITION0.5 mM H2O2 in agar) ------- COMMENT: 4bd2526203a7cc33 3 sN9oTuN7rp2vzh7feqryMQTbE5U Fig 4B; (comment: CONDITION0.5 mM H2O2 in agar) ------- COMMENT: 4bd2526203a7cc33 4 sN9oTuN7rp2vzh7feqryMQTbE5U Fig 4B; (comment: CONDITION0.5 mM H2O2 in agar) ------- COMMENT: 4bd2526203a7cc33 5 sN9oTuN7rp2vzh7feqryMQTbE5U Fig 4B; (comment: CONDITION0.5 mM H2O2 in agar) ------- COMMENT: 4bd2526203a7cc33 6 sN9oTuN7rp2vzh7feqryMQTbE5U Fig 4B; (comment: CONDITION0.5 mM H2O2 in agar) ------- COMMENT: 4bd2526203a7cc33 7 sN9oTuN7rp2vzh7feqryMQTbE5U Fig 4B; (comment: CONDITION0.5 mM H2O2 in agar) ------- COMMENT: 4bd2526203a7cc33 8 IdPvn7vojhqqqlLWAPCLN4pAUmU Fig 4B; (comment: CONDITION 1M KCl in agar) ------- COMMENT: 4bd2526203a7cc33 9 IdPvn7vojhqqqlLWAPCLN4pAUmU Fig 4B; (comment: CONDITION 1M KCl in agar) ------- COMMENT: 4bd2526203a7cc33 10 IdPvn7vojhqqqlLWAPCLN4pAUmU Fig 4B; (comment: CONDITION 1M KCl in agar) ------- COMMENT: 4bd2526203a7cc33 11 IdPvn7vojhqqqlLWAPCLN4pAUmU Fig 4B; (comment: CONDITION 1M KCl in agar) ------- COMMENT: 4bd2526203a7cc33 12 IdPvn7vojhqqqlLWAPCLN4pAUmU Fig 4B; (comment: CONDITION 1M KCl in agar) ------- COMMENT: 4bd2526203a7cc33 13 IdPvn7vojhqqqlLWAPCLN4pAUmU Fig 4B; (comment: CONDITION 1M KCl in agar) ------- COMMENT: 4bd2526203a7cc33 14 IdPvn7vojhqqqlLWAPCLN4pAUmU Fig 4B; (comment: CONDITION 1M KCl in agar) ------- COMMENT: 4bd2526203a7cc33 15 jE2iajuFOR4JEMawZFhqqubAiDY Fig. 6F ------- COMMENT: 4bd2526203a7cc33 16 /EKJDn1kiFepVVy1Uoj4agCFYLE Fig. 6E ------- COMMENT: 4bd2526203a7cc33 17 ecwX4alqEfu2i+w/vkmTFW1tmRs Fig. 7E ------- COMMENT: 4bd2526203a7cc33 18 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 4bd2526203a7cc33 19 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 4bd2526203a7cc33 20 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 4c042f68b675355e 1 gYzmIhMZK5dSFC8NkVpml26lDfs (comment: CONDITION 1.6 mM) ------- COMMENT: 4c042f68b675355e 2 gYzmIhMZK5dSFC8NkVpml26lDfs (comment: CONDITION 1.6 mM) ------- COMMENT: 4c718237e0521f89 18 N06byWWoTwKorzoZL4Xj4f6cd24 (comment: neutral wrt viability because data not shown, so don't know if aseptate mononucleate cells are the same ones that manage to survive and eventually divide) ------- COMMENT: 4ca3e303dba088f4 1 +nNAVDKjh7SBspstBReoeMySsto ------- COMMENT: 4ca3e303dba088f4 2 +nNAVDKjh7SBspstBReoeMySsto ------- COMMENT: 4ca3e303dba088f4 3 Zzv5GqssnnbHHChj8rVfcdQZUXY ------- COMMENT: 4ca3e303dba088f4 4 +nNAVDKjh7SBspstBReoeMySsto ------- COMMENT: 4ca3e303dba088f4 5 Zzv5GqssnnbHHChj8rVfcdQZUXY ------- COMMENT: 4ca3e303dba088f4 6 Zzv5GqssnnbHHChj8rVfcdQZUXY ------- COMMENT: 4ca3e303dba088f4 7 +nNAVDKjh7SBspstBReoeMySsto ------- COMMENT: 4ca3e303dba088f4 8 Zzv5GqssnnbHHChj8rVfcdQZUXY ------- COMMENT: 4cf5f407a9f3d7f 15 RPk1GLqIlbIt/3aQ19ccKmBGTq4 ------- COMMENT: 4d3b0d5a0015df91 1 U/PvCZ3/sYCTc+VkXd+IsT2uQcI fig 1b ------- COMMENT: 4d3b0d5a0015df91 2 U/PvCZ3/sYCTc+VkXd+IsT2uQcI fig 1b ------- COMMENT: 4d3b0d5a0015df91 3 U/PvCZ3/sYCTc+VkXd+IsT2uQcI fig 1b ------- COMMENT: 4d3b0d5a0015df91 4 U/PvCZ3/sYCTc+VkXd+IsT2uQcI fig 1b ------- COMMENT: 4d3b0d5a0015df91 5 wKE5RGUg8tMlZ5dNLHodHLKYsb4 Fig 1d ------- COMMENT: 4d3b0d5a0015df91 6 Ph/zIJtd4Zv8qSekboJt3wsN8JU Fig 1e ------- COMMENT: 4d3b0d5a0015df91 7 KCEnhsIw7N99i5dCU97nSmwve0U fig 1e ------- COMMENT: 4d3b0d5a0015df91 8 3IIZKd5m6W0pGR2ur3U7rMT0A9A fig 1d ------- COMMENT: 4d3b0d5a0015df91 9 /elVc9SrLTin96vGLwcnt406/is Fig. S1a ------- COMMENT: 4d3b0d5a0015df91 12 x/NZEmVqdHSTslB9O4JG1xKSqyY Fig. 2b ------- COMMENT: 4d3b0d5a0015df91 13 x/NZEmVqdHSTslB9O4JG1xKSqyY Fig. 2b ------- COMMENT: 4d3b0d5a0015df91 14 x/NZEmVqdHSTslB9O4JG1xKSqyY Fig. 2b ------- COMMENT: 4d3b0d5a0015df91 15 x/NZEmVqdHSTslB9O4JG1xKSqyY Fig. 2b ------- COMMENT: 4d3b0d5a0015df91 16 aIgNBGqVOoNFgelhklgDSvx1LYA fig 3a ------- COMMENT: 4d3b0d5a0015df91 17 aIgNBGqVOoNFgelhklgDSvx1LYA fig 3a ------- COMMENT: 4d3b0d5a0015df91 18 albsh4c4fJ5n6/A0pXFq63upAmc fig 3c ------- COMMENT: 4d3b0d5a0015df91 19 albsh4c4fJ5n6/A0pXFq63upAmc fig 3c ------- COMMENT: 4d3b0d5a0015df91 22 98E/IMrHCcm1LzmvLPDAVj5SZL8 (comment: CHECK separation) ------- COMMENT: 4d3b0d5a0015df91 24 cVFnXEVXpAP8GxWal6o8L1aLa0U fig 4c ------- COMMENT: 4d3b0d5a0015df91 25 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 4d3b0d5a0015df91 26 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 4d3b0d5a0015df91 27 MC1i373nFOtbM522r9/hDA+HaV0 Fig. 5b ------- COMMENT: 4d3b0d5a0015df91 28 MC1i373nFOtbM522r9/hDA+HaV0 Fig. 5b ------- COMMENT: 4d3b0d5a0015df91 29 5Pc+GJjJVhHm1h5Cm22Rhx2qUks fig 5B ------- COMMENT: 4d3b0d5a0015df91 31 qW0fooJwQWtfFzpmyf80rEliP/A fig 5c ------- COMMENT: 4d3b0d5a0015df91 32 MC1i373nFOtbM522r9/hDA+HaV0 Fig. 5b ------- COMMENT: 4d3b0d5a0015df91 33 1O121X2TIDttbodARbhHHl9uLQs Fig. 5c ------- COMMENT: 4d3b0d5a0015df91 34 rM8ewzRURtHbQL7oTbyXmtjS4BA fig 3a (comment: par1, the regulatory subunit was used in the assay) ------- COMMENT: 4d3b0d5a0015df91 35 aIgNBGqVOoNFgelhklgDSvx1LYA fig 3a ------- COMMENT: 4d5512a5dc8ea4bc 1 2koxBQNn71ba4vLHFIobG551/yY (comment: al: ubiquitin dependent due to need for rhp6) ------- COMMENT: 4d5512a5dc8ea4bc 2 2koxBQNn71ba4vLHFIobG551/yY (comment: al: ubiquitin dependent due to need for rhp6) ------- COMMENT: 4d5512a5dc8ea4bc 3 2koxBQNn71ba4vLHFIobG551/yY (comment: al: ubiquitin dependent due to need for rhp6) ------- COMMENT: 4d5512a5dc8ea4bc 14 DyR/d4+bPp3OfjmUeJA/weye0tQ fig 7c ------- COMMENT: 4d5512a5dc8ea4bc 34 JKT36y0ZnS514ZKfGAKQj0YrkZg ------- COMMENT: 4d56e9e82da23d80 3 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig 5 ------- COMMENT: 4d56e9e82da23d80 27 KhERRFlyccvk9OkFyZEhITQNiF0 Supp table ------- COMMENT: 4d56e9e82da23d80 28 4R0NudbE8uUYG6egwSbQaMePi2U (Figure 2c) ------- COMMENT: 4d56e9e82da23d80 29 4R0NudbE8uUYG6egwSbQaMePi2U (Figure 2c) ------- COMMENT: 4d56e9e82da23d80 30 hkAYTwXptZJW0cTNkvnkV5Y/U5s (Figure 2d) ------- COMMENT: 4d56e9e82da23d80 31 hkAYTwXptZJW0cTNkvnkV5Y/U5s (Figure 2d) ------- COMMENT: 4d56e9e82da23d80 32 FHkRXhIRTMUTituCJXGWi089Lbw figure 2E ------- COMMENT: 4d56e9e82da23d80 33 4R0NudbE8uUYG6egwSbQaMePi2U (Figure 2c) ------- COMMENT: 4d56e9e82da23d80 34 DRYJSGGCd2yYeqANMtUVaEW/nts (Figure 3a) ------- COMMENT: 4d56e9e82da23d80 35 POBMa9Oatn7Jwn0Y87SjVB93fFs (Figure 3a) ------- COMMENT: 4d56e9e82da23d80 36 POBMa9Oatn7Jwn0Y87SjVB93fFs (Figure 3a) ------- COMMENT: 4d56e9e82da23d80 40 POBMa9Oatn7Jwn0Y87SjVB93fFs (Figure 3a) ------- COMMENT: 4d56e9e82da23d80 49 27xZB0eF6Rtp80H35UxXIZO9u2k Figure S4 ------- COMMENT: 4d56e9e82da23d80 51 4R0NudbE8uUYG6egwSbQaMePi2U (Figure 2c) ------- COMMENT: 4d56e9e82da23d80 64 a64uQcnUtFAX7iDqW/sZkb7WwVc Figure 4C ------- COMMENT: 4d56e9e82da23d80 65 VBU1ZyXC4pXEDIG2K+BgMGO1BEU (Figure 4d) ------- COMMENT: 4d56e9e82da23d80 67 HbMNJ+aSiEAHCihlXjFGTqMpYUo (Figure 4c) ------- COMMENT: 4d56e9e82da23d80 68 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I (Figure 5) ------- COMMENT: 4d56e9e82da23d80 69 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I (Figure 5) ------- COMMENT: 4d56e9e82da23d80 70 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I (Figure 5) ------- COMMENT: 4d56e9e82da23d80 71 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I (Figure 5) ------- COMMENT: 4d56e9e82da23d80 72 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I (Figure 5) ------- COMMENT: 4d56e9e82da23d80 74 l+f1R919mLn1hkTFl9hvZaQF59A (Figure 6) ------- COMMENT: 4d56e9e82da23d80 75 l+f1R919mLn1hkTFl9hvZaQF59A (Figure 6) ------- COMMENT: 4d56e9e82da23d80 77 l+f1R919mLn1hkTFl9hvZaQF59A (Figure 6) ------- COMMENT: 4d56e9e82da23d80 78 l+f1R919mLn1hkTFl9hvZaQF59A (Figure 6) ------- COMMENT: 4d56e9e82da23d80 80 l+f1R919mLn1hkTFl9hvZaQF59A (Figure 6) ------- COMMENT: 4d56e9e82da23d80 81 l+f1R919mLn1hkTFl9hvZaQF59A (Figure 6) ------- COMMENT: 4d56e9e82da23d80 82 l+f1R919mLn1hkTFl9hvZaQF59A (Figure 6) ------- COMMENT: 4d56e9e82da23d80 83 l+f1R919mLn1hkTFl9hvZaQF59A (Figure 6) ------- COMMENT: 4d56e9e82da23d80 84 qJQOQ/HH0jvBSswX2GWnREAf5S4 Figure S8 ------- COMMENT: 4d56e9e82da23d80 85 qJQOQ/HH0jvBSswX2GWnREAf5S4 Figure S8 ------- COMMENT: 4d56e9e82da23d80 86 sbwpbdbx+S5J91GTX8D6Cz3gW5Y (Figure S8) ------- COMMENT: 4d56e9e82da23d80 87 ti8kkyjE5I1eJSh4+fw4ZP4vxjY (Figure S8) ------- COMMENT: 4d56e9e82da23d80 88 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: 4d56e9e82da23d80 89 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: 4d56e9e82da23d80 90 inwK+wHT89XPepaM+tannPK1NPQ Figure S7 ------- COMMENT: 4d56e9e82da23d80 91 evUKiEkXEk+1REsJMqEQquHJqfA (Figure S7) ------- COMMENT: 4d56e9e82da23d80 92 evUKiEkXEk+1REsJMqEQquHJqfA (Figure S7) ------- COMMENT: 4d9c10ab5ce2986c 1 qxK1gp7zLWgxwXLAOqR4xJdNYjg Movie 1A ------- COMMENT: 4d9c10ab5ce2986c 4 BrfIbYRWSQXRJuEBq4H5V6IodbY fig 2B ------- COMMENT: 4d9c10ab5ce2986c 5 zKxgyRAsYgWL7f58cuMZpRjtV+I fig 2B ------- COMMENT: 4d9c10ab5ce2986c 6 tLnGHxqrYCJT5+AHj3J18CNxzlo Figure 5b ------- COMMENT: 4d9c10ab5ce2986c 7 tLnGHxqrYCJT5+AHj3J18CNxzlo Figure 5b ------- COMMENT: 4d9c10ab5ce2986c 8 tLnGHxqrYCJT5+AHj3J18CNxzlo Figure 5b ------- COMMENT: 4d9c10ab5ce2986c 9 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 4dc5dac60241db83 25 OzpkKzzywoQw4fTfAp2QIltWuwk (comment: worse than cdc24-M38 alone -wt not shown) ------- COMMENT: 4dc5dac60241db83 26 OzpkKzzywoQw4fTfAp2QIltWuwk (comment: worse than cdc24-M38 alone -wt not shown) ------- COMMENT: 4dc5dac60241db83 27 OzpkKzzywoQw4fTfAp2QIltWuwk (comment: worse than cdc24-M38 alone -wt not shown) ------- COMMENT: 4dc5dac60241db83 28 OzpkKzzywoQw4fTfAp2QIltWuwk (comment: worse than cdc24-M38 alone -wt not shown) ------- COMMENT: 4dd289ca048623e6 13 RyAwOwo7AjKs4uXUjnkW8k2rqpc (comment: abolished interaction between wt and mutant; interaction partially restored if both copies are mutant) ------- COMMENT: 4dd289ca048623e6 14 RyAwOwo7AjKs4uXUjnkW8k2rqpc (comment: abolished interaction between wt and mutant; interaction partially restored if both copies are mutant) ------- COMMENT: 4dd289ca048623e6 61 Rn4DAauG7LAGBX1XyQOT9tEOjTI (comment: no extension because growth is decreased generally, making expressivity hard to judge) ------- COMMENT: 4dd289ca048623e6 62 Rn4DAauG7LAGBX1XyQOT9tEOjTI (comment: no extension because growth is decreased generally, making expressivity hard to judge) ------- COMMENT: 4dd289ca048623e6 63 Rn4DAauG7LAGBX1XyQOT9tEOjTI (comment: no extension because growth is decreased generally, making expressivity hard to judge) ------- COMMENT: 4dd289ca048623e6 64 Rn4DAauG7LAGBX1XyQOT9tEOjTI (comment: no extension because growth is decreased generally, making expressivity hard to judge) ------- COMMENT: 4dd289ca048623e6 65 Rn4DAauG7LAGBX1XyQOT9tEOjTI (comment: no extension because growth is decreased generally, making expressivity hard to judge) ------- COMMENT: 4dd289ca048623e6 66 Rn4DAauG7LAGBX1XyQOT9tEOjTI (comment: no extension because growth is decreased generally, making expressivity hard to judge) ------- COMMENT: 4dd289ca048623e6 72 Rn4DAauG7LAGBX1XyQOT9tEOjTI (comment: no extension because growth is decreased generally, making expressivity hard to judge) ------- COMMENT: 4dd289ca048623e6 73 Rn4DAauG7LAGBX1XyQOT9tEOjTI (comment: no extension because growth is decreased generally, making expressivity hard to judge) ------- COMMENT: 4dd289ca048623e6 74 Rn4DAauG7LAGBX1XyQOT9tEOjTI (comment: no extension because growth is decreased generally, making expressivity hard to judge) ------- COMMENT: 4dd289ca048623e6 75 Rn4DAauG7LAGBX1XyQOT9tEOjTI (comment: no extension because growth is decreased generally, making expressivity hard to judge) ------- COMMENT: 4dd289ca048623e6 76 Rn4DAauG7LAGBX1XyQOT9tEOjTI (comment: no extension because growth is decreased generally, making expressivity hard to judge) ------- COMMENT: 4dd289ca048623e6 77 Rn4DAauG7LAGBX1XyQOT9tEOjTI (comment: no extension because growth is decreased generally, making expressivity hard to judge) ------- COMMENT: 4de0124e8c719689 19 l+f1R919mLn1hkTFl9hvZaQF59A figure 6 ------- COMMENT: 4de0124e8c719689 25 T9uaeTqCYhA8EU0WkpUZ15olvkQ figure 6F ------- COMMENT: 4de0124e8c719689 26 T9uaeTqCYhA8EU0WkpUZ15olvkQ figure 6F ------- COMMENT: 4dfa9e1e417a7efe 21 zLKXAd/tsjV5aMhlQyPOmOWNS+g (comment: less sensitive than ssb3delta alone) ------- COMMENT: 4e4a8605a4b1fccb 59 Iqq8VSKYUq26P18grzYgkxOs1VU ------- COMMENT: 4e4fa8722ccab385 3 Qf5lXdAMZQGUWWyK/da/tksokd0 (comment: also assayed with GFP-NLS construct) ------- COMMENT: 4e4fa8722ccab385 7 aUAO9Rgypy/tqlRgh868Xqww2+Q (comment: can't use IPI because we don't have identifiers for human importin alpha or the GST-NLS construct) ------- COMMENT: 4e65ebe2b1bbb71f 2 DN97L+qtEMkzDcggALCLav0aG5k These results suggest that Epe1 promotes assembly of the RNAi machinery at constitutive heterochromatin by expressing dg/dh ncRNAs. ------- COMMENT: 4e7e30e382d662d9 14 Nds2+zlc9xBG9qdEiLhRIRoyAv0 ------- COMMENT: 4eb88108157577ae 6 U53n7la87w0Zs7yduRHaZkpJZCQ (comment: sfr1 protein is not stable without swi5. swi5 alone does not bind rad51.) ------- COMMENT: 4eb88108157577ae 15 U53n7la87w0Zs7yduRHaZkpJZCQ sfr1 protein is not stable without swi5. swi5 alone does not bind rad51. ------- COMMENT: 4eb88108157577ae 21 nxRnSwILvpgxeAVdzEQ9+mlVqiA (comment: in complex with Swi5) ------- COMMENT: 4eb88108157577ae 22 +ltlnWxrZo4m6k8M+GWS7t2q/FU (comment: in complex with Sfr1) ------- COMMENT: 4ebdf0faaedaf02f 20 BzDPaENJw7JW42w6IiUA77pnP+M (comment: CHECK telomerase regulator) ------- COMMENT: 4f106c6e34491953 1 yZXcpqGroL6E4whSBRXW9KfEI5w Table 1. Fig. 2A. Further observation of the germinating spores showed that the mutated h90 cells elongated and eventually divided, but never formed visible colonies (data not shown). ------- COMMENT: 4f106c6e34491953 2 p6y8TmoUcUga4UR08qnw0dYFARI Table 1. Further observation of the germinating spores showed that the mutated h90 cells elongated and eventually divided, but never formed visible colonies. Table 1 ------- COMMENT: 4f106c6e34491953 3 nsq7ePDwU4fEBV+HVVxPFTNeeiE Table 1. Further observation of the germinating spores showed that the mutated h90 cells elongated and eventually divided, but never formed visible colonies (data not shown). ------- COMMENT: 4f106c6e34491953 4 gr8Z1NJn5ieZQq/2m60IaI1FphU rhp22AD, rhp51D and rhp54D mutants do not form colonies in the wild-type h90 strain, as already shown for rhp22AD, although they are viable in mat1-Msmt-0 and mat1-PD17 backgrounds. ------- COMMENT: 4f106c6e34491953 5 gr8Z1NJn5ieZQq/2m60IaI1FphU rhp22AD, rhp51D and rhp54D mutants do not form colonies in the wild-type h90 strain, as already shown for rhp22AD, although they are viable in mat1-Msmt-0 and mat1-PD17 backgrounds. ------- COMMENT: 4f106c6e34491953 6 aPY+4QVlML+7MxI03Q9RxcIX/M8 rhp22AD, rhp51D and rhp54D mutants do not form colonies in the wild-type h90 strain, as already shown for rhp22AD, although they are viable in mat1-Msmt-0 and mat1-PD17 backgrounds. Fig. 2A ------- COMMENT: 4f106c6e34491953 7 gr8Z1NJn5ieZQq/2m60IaI1FphU rhp22AD, rhp51D and rhp54D mutants do not form colonies in the wild-type h90 strain, as already shown for rhp22AD, although they are viable in mat1-Msmt-0 and mat1-PD17 backgrounds. ------- COMMENT: 4f106c6e34491953 8 gr8Z1NJn5ieZQq/2m60IaI1FphU rhp22AD, rhp51D and rhp54D mutants do not form colonies in the wild-type h90 strain, as already shown for rhp22AD, although they are viable in mat1-Msmt-0 and mat1-PD17 backgrounds. ------- COMMENT: 4f106c6e34491953 9 gr8Z1NJn5ieZQq/2m60IaI1FphU rhp22AD, rhp51D and rhp54D mutants do not form colonies in the wild-type h90 strain, as already shown for rhp22AD, although they are viable in mat1-Msmt-0 and mat1-PD17 backgrounds. ------- COMMENT: 4f106c6e34491953 10 qumEeHgyG1iuGwgadCNou08sedg the h90 rad50D or h90 mre11D (data not shown) mutant forms small colonies. However, these colonies contain many dead cells and few spores. ------- COMMENT: 4f106c6e34491953 11 5ATpsGxox6Un1v+SsDXRQ8gE+oI the h90 rad50D or h90 mre11D (data not shown) mutant forms small colonies. However, these colonies contain many dead cells and few spores. Table 1. ------- COMMENT: 4f106c6e34491953 12 kloCZqzD8sRRtmLAgfN1zd9V/dU The h90 exo1D mutant is viable. Fig. 2B. Table 1 ------- COMMENT: 4f106c6e34491953 13 hEPCbhCi5eKDGq3fs9YA/K+bAc4 The h90 exo1D mutant is viable and does not exhibit MT switching defects. Fig. 2B. Table 1 ------- COMMENT: 4f106c6e34491953 14 RGhNxndPYGR0wX53aLArklyieWY the double h90 rad50D exo1D mutant is not viable, but can eventually form micro-colonies (Figure 2B, left panel). Table 1 ------- COMMENT: 4f106c6e34491953 15 5j0WlAGXeyMjltR5OmOX4lAdWXA The h90 rhp57D strain produces colonies with a mild defect in MT switching. Fig. 2C. Table 1 ------- COMMENT: 4f106c6e34491953 16 mkMScdOnADdrXyii9cQUSlGqVqo h90 swi5D mutant produces healthy colonies but MT switching is drastically reduced. Fig. 2C. Table 1 ------- COMMENT: 4f106c6e34491953 17 bcag2eUQ/qazzB2HNUvrCCz5HuQ However, the h90 rhp57D swi5D double mutant is not viable (Figure 2C). Table 1 ------- COMMENT: 4f106c6e34491953 18 KMB8pphjA/IF1DmIE184AgcJnSc Interestingly, upon re-streaking, the h 90 rhp57D cells progressively produced colonies defective in MT switching, indicating that Rhp57 participates in efficient MT switching. ------- COMMENT: 4f106c6e34491953 19 tXwefjh37R/6SjTd9JMaf4R1HuE As expected, mat1-Msmt-0 and mat1-PD17 strains, which do not exhibit SSBs, are fully viable regardless of the mutant status. ------- COMMENT: 4f106c6e34491953 20 tXwefjh37R/6SjTd9JMaf4R1HuE As expected, mat1-Msmt-0 and mat1-PD17 strains, which do not exhibit SSBs, are fully viable regardless of the mutant status. ------- COMMENT: 4f106c6e34491953 21 tXwefjh37R/6SjTd9JMaf4R1HuE As expected, mat1-Msmt-0 and mat1-PD17 strains, which do not exhibit SSBs, are fully viable regardless of the mutant status. ------- COMMENT: 4f106c6e34491953 22 tXwefjh37R/6SjTd9JMaf4R1HuE As expected, mat1-Msmt-0 and mat1-PD17 strains, which do not exhibit SSBs, are fully viable regardless of the mutant status. ------- COMMENT: 4f106c6e34491953 23 tXwefjh37R/6SjTd9JMaf4R1HuE As expected, mat1-Msmt-0 and mat1-PD17 strains, which do not exhibit SSBs, are fully viable regardless of the mutant status. ------- COMMENT: 4f106c6e34491953 24 tXwefjh37R/6SjTd9JMaf4R1HuE As expected, mat1-Msmt-0 and mat1-PD17 strains, which do not exhibit SSBs, are fully viable regardless of the mutant status. ------- COMMENT: 4f106c6e34491953 25 tXwefjh37R/6SjTd9JMaf4R1HuE As expected, mat1-Msmt-0 and mat1-PD17 strains, which do not exhibit SSBs, are fully viable regardless of the mutant status. ------- COMMENT: 4f106c6e34491953 26 tXwefjh37R/6SjTd9JMaf4R1HuE As expected, mat1-Msmt-0 and mat1-PD17 strains, which do not exhibit SSBs, are fully viable regardless of the mutant status. ------- COMMENT: 4f106c6e34491953 27 tXwefjh37R/6SjTd9JMaf4R1HuE As expected, mat1-Msmt-0 and mat1-PD17 strains, which do not exhibit SSBs, are fully viable regardless of the mutant status. ------- COMMENT: 4f106c6e34491953 28 tXwefjh37R/6SjTd9JMaf4R1HuE As expected, mat1-Msmt-0 and mat1-PD17 strains, which do not exhibit SSBs, are fully viable regardless of the mutant status. ------- COMMENT: 4f106c6e34491953 29 tXwefjh37R/6SjTd9JMaf4R1HuE As expected, mat1-Msmt-0 and mat1-PD17 strains, which do not exhibit SSBs, are fully viable regardless of the mutant status. ------- COMMENT: 4f106c6e34491953 30 tXwefjh37R/6SjTd9JMaf4R1HuE As expected, mat1-Msmt-0 and mat1-PD17 strains, which do not exhibit SSBs, are fully viable regardless of the mutant status. ------- COMMENT: 4f106c6e34491953 31 tXwefjh37R/6SjTd9JMaf4R1HuE As expected, mat1-Msmt-0 and mat1-PD17 strains, which do not exhibit SSBs, are fully viable regardless of the mutant status. ------- COMMENT: 4f106c6e34491953 32 tXwefjh37R/6SjTd9JMaf4R1HuE As expected, mat1-Msmt-0 and mat1-PD17 strains, which do not exhibit SSBs, are fully viable regardless of the mutant status. ------- COMMENT: 4f106c6e34491953 33 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 4f106c6e34491953 34 hLy9PCBT+EdaHCeQTRdu3VvVkyE Table 1. Fig. 3C and D ------- COMMENT: 4f106c6e34491953 35 hLy9PCBT+EdaHCeQTRdu3VvVkyE Table 1. Fig. 3C and D ------- COMMENT: 4f106c6e34491953 36 SWkfy3YeHytMNivk43H3k+MQXTY None of the single helicase and essential topoisome- rase 3 (in rqh1D background) mutant strains exhibit MT switching and/or viability defect, except for the pfh1 mutant where a mild MT switching defect was observed (Table I, data not shown). ------- COMMENT: 4f106c6e34491953 37 d5IrjhNQyHS5EyZuUXlpYNPw4Ag Table 1, Fig. 3A, C and D ------- COMMENT: 4f106c6e34491953 38 hLy9PCBT+EdaHCeQTRdu3VvVkyE Table 1. Fig. 3C and D ------- COMMENT: 4f106c6e34491953 39 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 4f106c6e34491953 40 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 4f106c6e34491953 41 NPvmomSMdlVsHoLTQv5AydFzoQw Table 1, Fig. 3A and B ------- COMMENT: 4f106c6e34491953 42 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 4f106c6e34491953 43 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 4f106c6e34491953 44 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 4f106c6e34491953 45 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 4f106c6e34491953 46 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 4f106c6e34491953 47 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 4f106c6e34491953 48 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 4f106c6e34491953 49 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 4f106c6e34491953 50 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 4f106c6e34491953 51 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 4f106c6e34491953 52 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 4f106c6e34491953 53 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 4f106c6e34491953 54 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 4f106c6e34491953 55 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 4f2995b7c74f1360 3 OzHUYNi8jTVqKROwdh3r1uhuuNc Fiig 6AB ------- COMMENT: 4f2995b7c74f1360 4 INyyalFSog2xVDY7sXhapZv3Fmc fig 6c ------- COMMENT: 4f2995b7c74f1360 6 Xnlh2Yz7+U32lnzACP/BC/Hk2Es fig 6AB ------- COMMENT: 4f2995b7c74f1360 12 RT+miFFwojZbVemJAizwEqqrSkA fig 1 C-E ------- COMMENT: 4f2995b7c74f1360 13 RT+miFFwojZbVemJAizwEqqrSkA fig 1 C-E ------- COMMENT: 4f2995b7c74f1360 14 QU9Y0uJyUorENhQQZqil+IGLPM0 fig 1B ------- COMMENT: 4f2995b7c74f1360 15 OAk+LS8dG6ZaErlfFVoulH1ZtzM fig 2H, 7A ------- COMMENT: 4f2995b7c74f1360 16 Vg36Pb3XgZZRHTX+vSWCnG6rMUM Fig 3B ------- COMMENT: 4f2995b7c74f1360 17 Vg36Pb3XgZZRHTX+vSWCnG6rMUM Fig 3B ------- COMMENT: 4f2995b7c74f1360 18 4dx9o8tsMs8kV0Qp0k79Zm9jlAY Fig S3B ------- COMMENT: 4f2995b7c74f1360 19 fNpmaE7MG19qjD0+PlLVAROJUuU fig 5a ------- COMMENT: 4f2995b7c74f1360 22 Xnlh2Yz7+U32lnzACP/BC/Hk2Es fig 6AB ------- COMMENT: 4f2995b7c74f1360 25 Xnlh2Yz7+U32lnzACP/BC/Hk2Es fig 6AB ------- COMMENT: 4f2995b7c74f1360 26 +YmWZBtF4r6G00V18ie7Psg8Rn8 Figure S4D ------- COMMENT: 4f2995b7c74f1360 27 ijdqFCag9KQIDccrQYZMmbQb1EM Fig 6E ------- COMMENT: 4f2995b7c74f1360 28 ijdqFCag9KQIDccrQYZMmbQb1EM Fig 6E ------- COMMENT: 4f2995b7c74f1360 29 ijdqFCag9KQIDccrQYZMmbQb1EM Fig 6E ------- COMMENT: 4f2995b7c74f1360 31 AjWRmFHqCbhLYkGxJiNcghXlTdk Fig S4E ------- COMMENT: 4f2995b7c74f1360 32 LDRTBFM+qvmdGiqIDA2BEEka150 Fig S4F and S4G ------- COMMENT: 4f2995b7c74f1360 34 fY4IavitxqcrS04nSV/wz48KhQM fig 7A ------- COMMENT: 4f2995b7c74f1360 35 WmTIJBliYvonlhuQl55CaJpxFYQ Fig 7C/D ------- COMMENT: 4f2995b7c74f1360 36 WmTIJBliYvonlhuQl55CaJpxFYQ Fig 7C/D ------- COMMENT: 4f2995b7c74f1360 37 THF83t7TFCg52RkpM/6DT301Pdw Fig 7D ------- COMMENT: 4f2995b7c74f1360 39 KwbgXoubBhjFig4BiRIv7+L/miQ Fig 7E ------- COMMENT: 4f2995b7c74f1360 40 KwbgXoubBhjFig4BiRIv7+L/miQ Fig 7E ------- COMMENT: 4f2995b7c74f1360 41 KwbgXoubBhjFig4BiRIv7+L/miQ Fig 7E ------- COMMENT: 4f2995b7c74f1360 43 Fbz1U7bCxi+V9lXdzb35kqQOhOk Fig S6A ------- COMMENT: 4f2995b7c74f1360 44 Fbz1U7bCxi+V9lXdzb35kqQOhOk Fig S6A ------- COMMENT: 4f2995b7c74f1360 45 pRSvGwhdbMPDSKEsJ9TtStypcok Fig S6 B (comment: probably due to delayed fusion of TRAPP containing vesicles with PM) ------- COMMENT: 4f2995b7c74f1360 46 AYqgM9COShtyKP0864HPpxpHK1Y Fig S6 C (comment: probably due to delayed fusion of TRAPP containing vesicles with PM) ------- COMMENT: 4f36d75cf334acbc 1 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig 1D ------- COMMENT: 4f36d75cf334acbc 2 FnlHGm4P5SsZ6no2x/1PN8x7DCU pdc2 is required for mRNA decapping. Fig. 2A 2B ------- COMMENT: 4f36d75cf334acbc 8 w70p743ctsqhtv1eDnDZmu6Vs70 Fig 6D ------- COMMENT: 4f36d75cf334acbc 9 w70p743ctsqhtv1eDnDZmu6Vs70 Fig 6D ------- COMMENT: 4f36d75cf334acbc 10 w70p743ctsqhtv1eDnDZmu6Vs70 Fig 6D ------- COMMENT: 4f36d75cf334acbc 11 w70p743ctsqhtv1eDnDZmu6Vs70 Fig 6D ------- COMMENT: 4f36d75cf334acbc 12 w70p743ctsqhtv1eDnDZmu6Vs70 Fig 6D ------- COMMENT: 4f36d75cf334acbc 22 i+pZZ1O9k+libAHisFcXjZ0t6y8 figure 1E ------- COMMENT: 4f36d75cf334acbc 23 i+pZZ1O9k+libAHisFcXjZ0t6y8 figure 1E ------- COMMENT: 4f36d75cf334acbc 24 jakyqE4bA5KDsNgxh1j6mFICJ8U figure 1D ------- COMMENT: 4f36d75cf334acbc 25 i+pZZ1O9k+libAHisFcXjZ0t6y8 figure 1E ------- COMMENT: 4f36d75cf334acbc 26 MRv4U0wptZfY8tXQDHMhzMNic0c figure 2B ------- COMMENT: 4f36d75cf334acbc 27 iyMraj5qmLEJtewo/fSEFxmllqQ figure 2D ------- COMMENT: 4f36d75cf334acbc 28 iyMraj5qmLEJtewo/fSEFxmllqQ figure 2D ------- COMMENT: 4f36d75cf334acbc 29 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 4f36d75cf334acbc 30 P6g3vUjg0BGYhq39R0tH7SVyWdw Fig. 4B,4D ------- COMMENT: 4f36d75cf334acbc 32 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 4f36d75cf334acbc 33 FxivaGe9PZmTfmK4Xq0JDXgNoUw Fig. 4H, right panel ------- COMMENT: 4f36d75cf334acbc 35 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 4f36d75cf334acbc 36 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 4f36d75cf334acbc 37 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 4f36d75cf334acbc 38 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: 4f36d75cf334acbc 40 14d7S4+i+cgbP0VZN+lpg//D4vs Fig. 5G, left panel ------- COMMENT: 4f36d75cf334acbc 41 14d7S4+i+cgbP0VZN+lpg//D4vs Fig. 5G, left panel ------- COMMENT: 4f36d75cf334acbc 42 14d7S4+i+cgbP0VZN+lpg//D4vs Fig. 5G, left panel ------- COMMENT: 4f36d75cf334acbc 43 M1A3l5LLBhyIrAwmJLOS5ZMcV1s Figure 6A ------- COMMENT: 4f36d75cf334acbc 44 YUIRK/dF8JSI25u/8oT5s0WAApY Figure 6C ------- COMMENT: 4f36d75cf334acbc 45 YUIRK/dF8JSI25u/8oT5s0WAApY Figure 6C ------- COMMENT: 4f36d75cf334acbc 46 w70p743ctsqhtv1eDnDZmu6Vs70 Fig 6D ------- COMMENT: 4f36d75cf334acbc 47 w70p743ctsqhtv1eDnDZmu6Vs70 Fig 6D ------- COMMENT: 4f36d75cf334acbc 48 w70p743ctsqhtv1eDnDZmu6Vs70 Fig 6D ------- COMMENT: 4f36d75cf334acbc 49 w70p743ctsqhtv1eDnDZmu6Vs70 Fig 6D ------- COMMENT: 4f36d75cf334acbc 50 w70p743ctsqhtv1eDnDZmu6Vs70 Fig 6D ------- COMMENT: 4f36d75cf334acbc 52 YeSKXTuBNlcZ1BPbB4mqa07PHuU figure 7A ------- COMMENT: 4f36d75cf334acbc 55 xbLdy4eUsRrl9dr1+JMIrCO09ew Fig 6C ------- COMMENT: 4f36d75cf334acbc 56 xbLdy4eUsRrl9dr1+JMIrCO09ew Fig 6C ------- COMMENT: 4f36d75cf334acbc 57 xbLdy4eUsRrl9dr1+JMIrCO09ew Fig 6C ------- COMMENT: 4f36d75cf334acbc 58 xbLdy4eUsRrl9dr1+JMIrCO09ew Fig 6C ------- COMMENT: 4f36d75cf334acbc 59 xbLdy4eUsRrl9dr1+JMIrCO09ew Fig 6C ------- COMMENT: 4f36d75cf334acbc 60 xbLdy4eUsRrl9dr1+JMIrCO09ew Fig 6C ------- COMMENT: 4f36d75cf334acbc 61 xbLdy4eUsRrl9dr1+JMIrCO09ew Fig 6C ------- COMMENT: 4f36d75cf334acbc 62 xbLdy4eUsRrl9dr1+JMIrCO09ew Fig 6C ------- COMMENT: 4f75e98b55c64601 3 5sH4zzEOT0RWgLK6h9jjs0AwH2I ------- COMMENT: 4f8815b38140d61d 6 /zmem2PQ2lZvMjOyYZujKvCYYng urg1, gar2, act1, adh1, pof9 and hcn1 mRNAs were shown to be direct targets by cRACE sequence analysis. ------- COMMENT: 4f955b5fd93e3ce0 1 heXNJPnr1mghSTwk5pwOJGKaa68 In addition, a low concentra- tion of TBZ enhanced the chromosome segregation de- fects of the temperature-sensitive sfh1-13 mutant (Supple- mentary Figure S1A). ------- COMMENT: 4f955b5fd93e3ce0 3 heXNJPnr1mghSTwk5pwOJGKaa68 In addition, a low concentra- tion of TBZ enhanced the chromosome segregation de- fects of the temperature-sensitive sfh1-13 mutant (Supple- mentary Figure S1A). ------- COMMENT: 4f955b5fd93e3ce0 4 7zNPMk76p1cpBk20iGxbvjzjzp8 sfh1-13 mutant strain exhibited a slow growth phenotype at 32◦C upon nmt41-cnp1 expression (sfh1-13 at 32◦C in the lower panel of Supplementary Figure S1B ------- COMMENT: 4f955b5fd93e3ce0 5 jvU3VXLTFRMrZ7aw8k1FkGuGtag However, deletion strains of two genes encod- ing SWI/SNF core components, snf5 and snf22, did not exhibit sensitivity to CENP-ACnp1 overexpression (Supple- mentary Figure S2A). ------- COMMENT: 4f955b5fd93e3ce0 6 jvU3VXLTFRMrZ7aw8k1FkGuGtag However, deletion strains of two genes encod- ing SWI/SNF core components, snf5 and snf22, did not exhibit sensitivity to CENP-ACnp1 overexpression (Supple- mentary Figure S2A). ------- COMMENT: 4f955b5fd93e3ce0 7 plk07gNln+1if7zR7KdNuHTqd50 at pericentromeric heterochromatin When CENP-ACnp1 was expressed at wild- type levels, specific accumulation at pericentromeric heterochromatin domains of all centromeres (Figure 1A), but not at non-centromeric locations ------- COMMENT: 4f955b5fd93e3ce0 8 pHWI0963VO6MTpI5Wtf4c5hnUqM Importantly, no significant dif- ference in the level of Cnp1 protein or mRNA was seen in sfh1-13 cells (Figure 1E). ------- COMMENT: 4f955b5fd93e3ce0 9 plk07gNln+1if7zR7KdNuHTqd50 at pericentromeric heterochromatin When CENP-ACnp1 was expressed at wild- type levels, specific accumulation at pericentromeric heterochromatin domains of all centromeres (Figure 1A), but not at non-centromeric locations ------- COMMENT: 4f955b5fd93e3ce0 10 Dby0jfydfSP6JQC83++Op4cqmFg Furthermore, we observed ectopic CENP-ACnp1 deposition at pericentromeric heterochromatin domains in snf21-36 but not in rsc1 and rsc4 deletion mutants (Figure 1F) ------- COMMENT: 4f955b5fd93e3ce0 13 Y0G9AeOJsiRfI+mLjF3Jvy8N6bQ However, the sfh1-13 mutation had only a mild influence on H3K9me levels (Figure 1C), as we re- ported previously (12). ------- COMMENT: 4f955b5fd93e3ce0 15 qmckmtjsktkn6x1IJbDelDwTPtI In sfh1-13 mutant cells, a small but signifi- cant increase in the localization of CENP-CCnp3 and CENP- IMis6 was observed at surrounding pericentromeric repeats (Figure 2B and C); ------- COMMENT: 4f955b5fd93e3ce0 16 qmckmtjsktkn6x1IJbDelDwTPtI In sfh1-13 mutant cells, a small but signifi- cant increase in the localization of CENP-CCnp3 and CENP- IMis6 was observed at surrounding pericentromeric repeats (Figure 2B and C); ------- COMMENT: 4f955b5fd93e3ce0 17 TWF3sGA2w9xDmZLF20Livz9/Ad8 sfh1-13 clr3Δ cells were faster growing on TBZ-containing plates than sfh1-13 cells, indicating that deletion of clr3 partially rescues the TBZ sensitivity of sfh1-13. ------- COMMENT: 4f955b5fd93e3ce0 18 pEfcDb+7Vkvdq5++/lrZYz10v68 while loss of Clr3 eliminated CENP-ACnp1 accumulation at the pericentromere in a sfh1- 13 mutant background, indicating that Clr3 is required for ectopic deposition of CENP-ACnp1 (Figure 3D). ------- COMMENT: 4f955b5fd93e3ce0 19 EomOTtph5lAVNdQJi4SJvp3EqwY (Figure 3E). As reported previously, deletion of clr3 increased the loading of Snf21 at pericentromeric repeats; however, we did not observe greater Snf21 occupancy in our sfh1-13 clr3Δ double mutant relative to a sfh1-13 sin- gle mutant, suggesting that sfh1-13 prevents the acetylation- dependent recruitment of Snf21 at pericentromeres, and that the elimination of misloaded CENP-ACnp1 in sfh1-13Δ clr3Δ cells is not due to the increase in Snf21 level. ------- COMMENT: 4f955b5fd93e3ce0 20 f1jT5LvSI+NJEhXAT45vOHX7pUc Importantly, MNase protection at sites i, iii, and v, which are located between CENP-ACnp1 and heterochro- matin domains, was elevated in sfh1-13 cells (site i: 1.5-fold, P-value = 0.027; site iii: 2.2-fold, P-value = 0.0001; site v: 1.2-fold, P-value = 0.02; left panel of Figure 5B), indicat- ing that Sfh1/RSC contributes to chromatin decompaction at the boundary. ------- COMMENT: 4fa1a879e9d78db6 1 GDTvNUQ26T9qM9STrdBdGXyuuyE Fig 2,3 ------- COMMENT: 4fa1a879e9d78db6 2 h09KQpN7u/q6m3yDchMr2e10lc8 Fig4 ------- COMMENT: 4fa1a879e9d78db6 3 h09KQpN7u/q6m3yDchMr2e10lc8 Fig4 ------- COMMENT: 4fa1a879e9d78db6 4 h09KQpN7u/q6m3yDchMr2e10lc8 Fig4 ------- COMMENT: 4fa1a879e9d78db6 5 bQrAvw5v3pdeGEjTasNZSNQl2dw Figure 6A ------- COMMENT: 4fa1a879e9d78db6 6 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 4fa1a879e9d78db6 7 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 4fa1a879e9d78db6 8 bQrAvw5v3pdeGEjTasNZSNQl2dw Figure 6A ------- COMMENT: 4fa1a879e9d78db6 9 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 4fa1a879e9d78db6 12 vFCY2hQN61n2pE4UJl1RbQx0r3Y Figure 7 (this protrusion is opposite side of nucleus to the SPB) ------- COMMENT: 4fa1a879e9d78db6 13 vFCY2hQN61n2pE4UJl1RbQx0r3Y Figure 7 (this protrusion is opposite side of nucleus to the SPB) ------- COMMENT: 4fa485c82556641b 3 W1IV6zBsYsMKdB2fXE9Xw+kjSu8 Figure 1A (comment:increased size?) ------- COMMENT: 4fa485c82556641b 4 NhkOKYbCh4Xk5344C0hpf+HgaEU Figure 1B ------- COMMENT: 4fa485c82556641b 5 BJ79CCdIs9uCpO29r+29Uejo1Lo Figure 1A ------- COMMENT: 4fa485c82556641b 6 JJ61Rr+BzZ+yYkS4ndaMB26Ouxc Figure 2B ------- COMMENT: 4fa485c82556641b 7 JJ61Rr+BzZ+yYkS4ndaMB26Ouxc Figure 2B ------- COMMENT: 4fa485c82556641b 8 JJ61Rr+BzZ+yYkS4ndaMB26Ouxc Figure 2B ------- COMMENT: 4fa485c82556641b 9 JJ61Rr+BzZ+yYkS4ndaMB26Ouxc Figure 2B ------- COMMENT: 4fa485c82556641b 11 2kuSN7rMzfGcB2DKt67EqDWQELA Figure 2 ------- COMMENT: 4fa485c82556641b 13 mo4lJlEevU73nNg7342dcsBJBHE Figure 3A ------- COMMENT: 4fa485c82556641b 14 TATzzjxw0++zTSipiayyoyyybWU Figure 3B ------- COMMENT: 4fa485c82556641b 15 TATzzjxw0++zTSipiayyoyyybWU Figure 3B ------- COMMENT: 4fa485c82556641b 16 TATzzjxw0++zTSipiayyoyyybWU Figure 3B ------- COMMENT: 4fa485c82556641b 17 TATzzjxw0++zTSipiayyoyyybWU Figure 3B ------- COMMENT: 4fa485c82556641b 18 TATzzjxw0++zTSipiayyoyyybWU Figure 3B ------- COMMENT: 4fa485c82556641b 19 /A8yRiCY+eNZFl4L/mLnXXU2oJQ Figure 4C ------- COMMENT: 4fa485c82556641b 20 4I3tgOFS1RNU1tbe+jPpcGlFAYA (comment: CHECK 5.6%) ------- COMMENT: 4fa485c82556641b 30 uP2b450kx3y/u6z3D7bDUa+eEuM fig8 ------- COMMENT: 4fb0ad7bc2d593e0 34 ZT5D3yEDPr4VqhQHkIRdkMKFYkI ------- COMMENT: 4fb0ad7bc2d593e0 37 ZT5D3yEDPr4VqhQHkIRdkMKFYkI ------- COMMENT: 4fb0ad7bc2d593e0 39 ZT5D3yEDPr4VqhQHkIRdkMKFYkI ------- COMMENT: 4fb0ad7bc2d593e0 40 ZT5D3yEDPr4VqhQHkIRdkMKFYkI ------- COMMENT: 4fb0ad7bc2d593e0 42 ZT5D3yEDPr4VqhQHkIRdkMKFYkI ------- COMMENT: 4fb0ad7bc2d593e0 68 ZT5D3yEDPr4VqhQHkIRdkMKFYkI ------- COMMENT: 4fc60b01472378b1 1 3EfvjaMCp77T+8C8sptTG84+fIc Fig1B ------- COMMENT: 4fc60b01472378b1 2 3EfvjaMCp77T+8C8sptTG84+fIc Fig1B ------- COMMENT: 4fc60b01472378b1 3 3EfvjaMCp77T+8C8sptTG84+fIc Fig1B ------- COMMENT: 4fc60b01472378b1 4 3EfvjaMCp77T+8C8sptTG84+fIc Fig1B ------- COMMENT: 4fc60b01472378b1 5 3EfvjaMCp77T+8C8sptTG84+fIc Fig1B ------- COMMENT: 4fc60b01472378b1 6 3EfvjaMCp77T+8C8sptTG84+fIc Fig1B ------- COMMENT: 4fc60b01472378b1 7 3EfvjaMCp77T+8C8sptTG84+fIc Fig1B ------- COMMENT: 4fc60b01472378b1 8 3EfvjaMCp77T+8C8sptTG84+fIc Fig1B ------- COMMENT: 4fc60b01472378b1 9 kkqFcl5g22188VumjbXXbjb8YBQ data not shown 8 fold increase in response to res1 oe. res1 and lacZ fusion on episomal plasmids ------- COMMENT: 4fc60b01472378b1 10 sF4AliAh0qYsKpBkwBMvRN+OVBA Data not shown 20 fold increase in response to res1 oe. res1 and lacZ fusion on episomal plasmids ------- COMMENT: 4fc60b01472378b1 12 hDfZ//1tN2sZz4ICDfKu9/D46lk Fig1C Shows 5 fold increase in presence of HU compared to no HU. res1 and lacZ fusion on episomal plasmids ------- COMMENT: 4fc60b01472378b1 13 ETFHufhKFz7HpOd2ONrEKfpTzbc Fig1C no increase in presence of HU compared to no HU when all MCB elements are removed. res1 and lacZ fusion on episomal plasmids ------- COMMENT: 4fc60b01472378b1 15 kBeVQsj57U78Q+MBFzv1FHVzZs8 Fig1C 5 fold increase in HU compared to no HU. res1 and lacZ fusion on episomal plasmids ------- COMMENT: 4fc60b01472378b1 16 IEXOkiUWzOm9cj/LI9e0Pz8MkSI Fig2B ------- COMMENT: 4fc60b01472378b1 17 IEXOkiUWzOm9cj/LI9e0Pz8MkSI Fig2B ------- COMMENT: 4fc60b01472378b1 18 IEXOkiUWzOm9cj/LI9e0Pz8MkSI Fig2B ------- COMMENT: 4fc60b01472378b1 19 IEXOkiUWzOm9cj/LI9e0Pz8MkSI Fig2B ------- COMMENT: 4fc60b01472378b1 20 oT/OcXiSsH6RWhVLJJEUtUifcUE Fig3A ------- COMMENT: 4fc60b01472378b1 21 psJbSkNdsDHlhumDf18+Zf6KWvI Fig3B ------- COMMENT: 4fc60b01472378b1 22 psJbSkNdsDHlhumDf18+Zf6KWvI Fig3B ------- COMMENT: 4fc60b01472378b1 24 Ozp47kvNh5k2BQB6BeY/MCsDsaY Fig4D ------- COMMENT: 4fc60b01472378b1 25 Ozp47kvNh5k2BQB6BeY/MCsDsaY Fig4D ------- COMMENT: 4fc60b01472378b1 26 Ozp47kvNh5k2BQB6BeY/MCsDsaY Fig4D ------- COMMENT: 4fc60b01472378b1 27 2VHeTeAcCPcgYouEd5MmGorxfP0 FIg4E ------- COMMENT: 4fc60b01472378b1 28 qegAgQPz+f8iPB1lXi3zdoYuNV0 Fig 5 res1-S130A can rescue the pat1-114 mutant at low levels of over expression ------- COMMENT: 4fc60b01472378b1 29 TM+1igLnCOAWfKoQhn+YKdQtF+c Fig5 res1+ is unable to rescue the pat1-114 mutant at low levels of over expression ------- COMMENT: 4fc60b01472378b1 30 3V3HaMQsZES9F/qR1JrWhOrHbFU Fig6 ------- COMMENT: 4fc60b01472378b1 31 bc5ysz+g2kJpGR/n0tJxwXYHZ4Y Fig7B res1S130A prevents the normal down regulation of MBF dependent transcription by res1+ ------- COMMENT: 4fc60b01472378b1 32 bc5ysz+g2kJpGR/n0tJxwXYHZ4Y Fig7B res1S130A prevents the normal down regulation of MBF dependent transcription by res1+ ------- COMMENT: 4fc60b01472378b1 33 bc5ysz+g2kJpGR/n0tJxwXYHZ4Y Fig7B res1S130A prevents the normal down regulation of MBF dependent transcription by res1+ ------- COMMENT: 4fc60b01472378b1 34 IEXOkiUWzOm9cj/LI9e0Pz8MkSI Fig2B ------- COMMENT: 4fc60b01472378b1 35 IEXOkiUWzOm9cj/LI9e0Pz8MkSI Fig2B ------- COMMENT: 500e6c06108b2c75 1 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2A ------- COMMENT: 500e6c06108b2c75 2 FJrUrY/cNbL7O7xtjP6w5yuBdIM fig 2B ------- COMMENT: 500e6c06108b2c75 3 FJrUrY/cNbL7O7xtjP6w5yuBdIM fig 2B ------- COMMENT: 500e6c06108b2c75 4 iXHIAi0t+dy4Wg/hrg7PPdT1qxY fig 2E ------- COMMENT: 500e6c06108b2c75 7 glJW1aOyj4jhjS/C9sk9B+zU/dU Fig 4a ------- COMMENT: 500e6c06108b2c75 8 glJW1aOyj4jhjS/C9sk9B+zU/dU Fig 4a ------- COMMENT: 500e6c06108b2c75 9 MoOViw2M3RRD0iSsP9Vt85D+e80 Fig 4ab ------- COMMENT: 500e6c06108b2c75 10 EL3a+zN26VpZTNFcjU2XCRt0XzQ Fig 4de ------- COMMENT: 500e6c06108b2c75 11 l+f1R919mLn1hkTFl9hvZaQF59A Fig fig6 ------- COMMENT: 5029f97c642fcc70 1 9OAGxzNQy+9Z+Zpw9vNnYMEBTOQ A novel 5′-hydroxyl dinucleotide hydrolase activity for the DXO/Rai1 family of enzymes ------- COMMENT: 50345fb1226215ae 3 Atx2F643irDaDxE5EYK2AiiSLFw Figure 1A. We concluded that the severe phenotype of a ®n1.ts1 mutant is a transitory response to loss of Fin1 function. This implied that ®n1.D haploids adapted to loss of Fin1 after the ®rst division of a germinating spore. ------- COMMENT: 50345fb1226215ae 4 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 50345fb1226215ae 5 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 50345fb1226215ae 6 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 50345fb1226215ae 14 SJvD0R7KoFjTCbabRjs/mcP/jp0 Figure 2B and C ------- COMMENT: 50345fb1226215ae 15 t1I4cyr7/mvwujHjE4oIcUprzNw Figures 1C and 2C ------- COMMENT: 50345fb1226215ae 16 z1HIe0rLIh6zwg5V2lOg/ky0n3E (comment: recessive, loss- of-function mutation) ------- COMMENT: 50345fb1226215ae 17 uTHYYgAwer1GcF/BcZX7oR1OJU8 Figure 2A; Table II ------- COMMENT: 50345fb1226215ae 18 z1HIe0rLIh6zwg5V2lOg/ky0n3E (comment: recessive, loss- of-function mutation) ------- COMMENT: 50345fb1226215ae 19 ebBLS6hR9ufILSIBuAwa1xut5M8 Table II ------- COMMENT: 50345fb1226215ae 20 ebBLS6hR9ufILSIBuAwa1xut5M8 Table II ------- COMMENT: 50345fb1226215ae 23 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: 50345fb1226215ae 24 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: 50345fb1226215ae 25 m6DRiW8UcQAAODplAQGjn4Gesr8 figure 5d ------- COMMENT: 50345fb1226215ae 26 qZGYiUGOg5vLjoQZHE0I7Ta/QcA (comment: CONDITION 25 degrees) figure 5d ------- COMMENT: 50345fb1226215ae 27 mw2dv5Q8xSHVyv2T9mEAeOhMhD8 (comment: CONDITION 25 degrees) figure 7 ------- COMMENT: 50345fb1226215ae 28 mw2dv5Q8xSHVyv2T9mEAeOhMhD8 (comment: CONDITION 25 degrees) figure 7 ------- COMMENT: 50345fb1226215ae 29 cRQoILCBYx9bcqNL7QfYTRbCXtk Introduction of either ®n1.ts1 or ®n1.D to a cut12.s11 cdc25.22 mutant background abolished the growth of cdc25.22 above 30°C that had been conferred by the cut12.s11 mutation (Figure 6C). ------- COMMENT: 50574c5aacc2f341 1 ZEDdJPoZzAEO3N4nB905moc/LG0 Vsl1p is a partner of Pep12p, and mainly functions on the prevacuolar and vacuolar membrane. ------- COMMENT: 50574c5aacc2f341 5 5Db0qFXz+KVOA9vi/0AVz/spcY8 (comment: Sensitive to 3 mM ZnCl2. Suppressed by overexpression of budding yeast VAM7.) ------- COMMENT: 50574c5aacc2f341 7 yD13wxk8KHTG+DFUDkVlsCTV/O0 (comment: Sensitive to 3 mM ZnCl2) ------- COMMENT: 50574c5aacc2f341 10 iDjpiXvD6sKo54uu/ReIE+3StUI mutants defective in vacuolar sorting do not deliver SpCPY to the vacuole but rather to the outside of the cells. ------- COMMENT: 50574c5aacc2f341 12 iDjpiXvD6sKo54uu/ReIE+3StUI mutants defective in vacuolar sorting do not deliver SpCPY to the vacuole but rather to the outside of the cells. ------- COMMENT: 50574c5aacc2f341 19 ZEDdJPoZzAEO3N4nB905moc/LG0 Vsl1p is a partner of Pep12p, and mainly functions on the prevacuolar and vacuolar membrane. ------- COMMENT: 505fabad6506e06e 1 QaXJikH0G9D/+Vk5MiUc8feUcAk (comment: at late-firing or dormant origins; genome-wide detection) ------- COMMENT: 505fabad6506e06e 2 ewfspod7Bin5Lx5/B/NV8EarpfI (comment: CONDITION 30 degrees) ------- COMMENT: 505fabad6506e06e 3 ewfspod7Bin5Lx5/B/NV8EarpfI (comment: 30 degrees) ------- COMMENT: 505fabad6506e06e 4 9r6jpejM/rwYANTnxF24Sa98+EE (comment: actually 25 degrees, but calling it low to make distinction from inviable at 30) ------- COMMENT: 505fabad6506e06e 28 aBB9znlM9H/4BWpW5BVuRU0k0AQ (comment: assayed using ars2004; not abolished as in hsk1delta alone (but single mutant not shown)) ------- COMMENT: 505fabad6506e06e 29 aBB9znlM9H/4BWpW5BVuRU0k0AQ (comment: assayed using ars2004; not abolished as in hsk1delta alone (but single mutant not shown)) ------- COMMENT: 505fabad6506e06e 32 WoFP2YuplgW7vkSHXCWpNwd6NbI (comment: 30 degrees, "high" compared to 25 degrees) ------- COMMENT: 505fabad6506e06e 34 T1+JvPZ9UOn4K3ud6VC3zjjunTg ------- COMMENT: 505fabad6506e06e 38 QaXJikH0G9D/+Vk5MiUc8feUcAk (comment: at late-firing or dormant origins) ------- COMMENT: 505fabad6506e06e 44 T1+JvPZ9UOn4K3ud6VC3zjjunTg ------- COMMENT: 505fabad6506e06e 46 hLXCv1xMrkl+Zg8jorNORXFIwgU (comment: early-firing origins; HU absent) ------- COMMENT: 508a767204d31a98 1 Fbpdn9+JkFhamw2wwL63aQlfl90 (comment: assayed using myelin basic protein; doesn't rule out tyrosine phosphorylation) ------- COMMENT: 508a767204d31a98 3 UN2c4xHsBpRehfx+/yhH56Yzlrs (comment: CHECK based just on this paper, candidate for involved_in_or_regulates qualifier) ------- COMMENT: 508a767204d31a98 34 7+cKrH05PV83g/zGgDCsXcG8Euw ------- COMMENT: 50905b15a05554bd 16 C8cj+39NHRNESMi8XE9hjJq7J3k (comment: Cdc42-GTP assayed with CRIB) ------- COMMENT: 50905b15a05554bd 30 C8cj+39NHRNESMi8XE9hjJq7J3k (comment: Cdc42-GTP assayed with CRIB) ------- COMMENT: 50905b15a05554bd 38 C8cj+39NHRNESMi8XE9hjJq7J3k (comment: Cdc42-GTP assayed with CRIB) ------- COMMENT: 50905b15a05554bd 43 C8cj+39NHRNESMi8XE9hjJq7J3k (comment: Cdc42-GTP assayed with CRIB) ------- COMMENT: 5097e18e203bd23d 3 JmKeDxQWD/RDIcvhG+S+2KPuc1U Northern blot analysis on RNA from these cells usingmeu10 + cDNA as a probe revealed that meu10+ was tran-scribed during meiosis, even in mei4-null mutant cells(Fig. 1D). ------- COMMENT: 5097e18e203bd23d 4 jchSsEQO6ebyuTCMPOum+lBaC/4 Thus,the meu10+ gene is not essential for vegetative growth. ------- COMMENT: 5097e18e203bd23d 5 zfurzyHu4A8ltYjM33ikHuelMA8 No mature ascospores were observed in the meu10∆mutant (Fig. 3C and see Fig. 4A). ------- COMMENT: 5097e18e203bd23d 6 zfurzyHu4A8ltYjM33ikHuelMA8 No mature ascospores were observed in the meu10∆mutant (Fig. 3C and see Fig. 4A). ------- COMMENT: 5097e18e203bd23d 7 x2T5DRtqb69gxmLPzGPG0MlKPmY We next measured the DNA content of the meu10∆ and wild-type cells after nitrogenstarvation by flow cytometry. No apparent differencewas observed (data not shown), which indicates thatMeu10 is not required for pre-meiotic DNA synthesis.No mature ascospores were observed in the meu10∆mutant (Fig. 3C and see Fig. 4A). ------- COMMENT: 5097e18e203bd23d 8 do1qaSQ5yxzAIbAHIeLue2Uqwu0 ell and nucleishapes in meu10∆ cells during the horse-tail, meiosisI and meiosis II stages were normal (data not shownand uppermost panel at 8 h in Fig. 4A), ------- COMMENT: 5097e18e203bd23d 11 ynT+zn92GPnHVpFZp4suXehh+R8 Moreover, the thickness of the walls of the meu10∆spores was not homogeneous. In some parts, the sporewall was approximately threefold thicker than wild-type spore walls and seemed to be composed of sparsematerial, whereas in other parts, they were abnormallythin and looked very weak. The enlarged image of anabnormal ascospore shows a scattered stripe structureacross the spore wall (Fig. 5C) that is not observed in thewall of wild-type ascospores. The abnormal spore wallseems fragile, as some of them appear to be broken,allowing the cytoplasmic material to leak out. In addi-tion, at the later time point (22 h), most of the ascosporewalls disappeared. This fragility of the ascospore walls islikely to be why the meu10∆ spore nuclei are stainingabnormally with Hoechst33342 (as seen in Fig. 4A). ------- COMMENT: 5097e18e203bd23d 12 /3wI+F68KLvrunMvYGhxEhdQ8MM At meiosis II, however, Meu10-GFP appearedthroughout the cytoplasm and displayed a homogene-ous subcellular distribution (Fig. 6Aiv). As sporulationcommenced and progressed, the Meu10-GFP proteingradually moved to the peripheral region of the spores(Fig. 6Av), finally being distributed as a ring around eachof the four spores (Fig. 6Avi). Following sporulation, theGFP signal relocalized into the cytoplasm, where it wasfound as punctuated granules (Fig. 6Avii). ------- COMMENT: 5097e18e203bd23d 13 x5qnUq0IOm0Kbm0o7UA9JELNXqo As visualized by the goldparticles, 1,3-β-glucan was found at the inner layer ofthe spore wall in the wild-type ascospores but in meu10∆ascospores it was scattered throughout the spore wall(Fig. 7A).Thus, Meu10 is required for the accurate local-ization of 1,3-β-glucan in the spore wall ------- COMMENT: 5097e18e203bd23d 16 VLZxHUBDGIwngSScexe4Yzd8lv8 Like the meu10∆mutant (Fig. 3C), mature ascospores were not generatedby this mutant. ------- COMMENT: 5097e18e203bd23d 18 zfurzyHu4A8ltYjM33ikHuelMA8 No mature ascospores were observed in the meu10∆mutant (Fig. 3C and see Fig. 4A). ------- COMMENT: 5097e18e203bd23d 19 RQehdCJorfFYotQ7Jx1mcWpG9pc In this mutant, all the spores wereunviable and only faint signals from Meu10-C120∆-GFP were detected. (Fig. 8B, Cii) ------- COMMENT: 5097e18e203bd23d 20 sa8j5ccFk6ip97RNW+UPz24EAZM Moreover, Meu10-N117∆-GFP signals in the abnormally shaped ascospores werenot detected (Fig. 8Ciii), indicating that this domain isessential for the spore wall localization of Meu10 ------- COMMENT: 5097e18e203bd23d 21 M68+drFgqCKaQc20cA8T1vpo8rg Like the meu10∆ mutant, the spores from this mutantwere not viable (Fig. 8B) ------- COMMENT: 50980b7b866ebd46 1 TzRTgHgshiCFxEM9tL4eV3/mKfY Rgf1p, a Rho1p-specific GEF, is a new member of the Pmk1p MAPK pathway in fission yeast. ------- COMMENT: 50980b7b866ebd46 2 gZNo/xWKvKsHWWGOazkQEqseH2U (Fig. 1) ------- COMMENT: 50980b7b866ebd46 3 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: 50980b7b866ebd46 4 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: 50980b7b866ebd46 5 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: 50980b7b866ebd46 6 7SsdUnqRaZ6NYgeLuV84sMHM4k4 (Fig. 2D and Fig. 3B) ------- COMMENT: 50980b7b866ebd46 7 7SsdUnqRaZ6NYgeLuV84sMHM4k4 (Fig. 2D and Fig. 3B) ------- COMMENT: 50980b7b866ebd46 8 7SsdUnqRaZ6NYgeLuV84sMHM4k4 (Fig. 2D and Fig. 3B) ------- COMMENT: 50980b7b866ebd46 9 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: 50980b7b866ebd46 10 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: 50980b7b866ebd46 11 3eUaPxXLnSC2xPw264rWB26+35o (Fig. 1, Fig. 2D and Fig. 3) ------- COMMENT: 50980b7b866ebd46 12 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: 50980b7b866ebd46 13 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: 50980b7b866ebd46 14 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: 50980b7b866ebd46 15 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: 50980b7b866ebd46 16 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 50980b7b866ebd46 17 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 50980b7b866ebd46 18 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 50980b7b866ebd46 19 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 50980b7b866ebd46 20 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 50980b7b866ebd46 21 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 50980b7b866ebd46 22 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 50980b7b866ebd46 23 GkI93EYSqP6NybAtsJjxO2fy3Ic (Fig. 4D) ------- COMMENT: 50980b7b866ebd46 24 MfhXrratyk9HEpPvYNKyPqJLEoI (Fig. 4E) ------- COMMENT: 50980b7b866ebd46 25 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: 50980b7b866ebd46 26 kuRdbpnFrU9sjksgW9GQwEQ1424 (Fig. 5) ------- COMMENT: 50980b7b866ebd46 27 kuRdbpnFrU9sjksgW9GQwEQ1424 (Fig. 5) ------- COMMENT: 50980b7b866ebd46 28 hPFNK9pXn9s6uyKJPTkg3DIM2XE (Fig. 6A) ------- COMMENT: 50980b7b866ebd46 29 wnx2gS3IAKqzPkStlyJ0uF9GveM (Fig. 6C) ------- COMMENT: 50980b7b866ebd46 30 XwjFKIBpFnAfCL+3uv8ZHt+X4Cc (Fig. 6D) ------- COMMENT: 50980b7b866ebd46 31 XwjFKIBpFnAfCL+3uv8ZHt+X4Cc (Fig. 6D) ------- COMMENT: 50980b7b866ebd46 32 XwjFKIBpFnAfCL+3uv8ZHt+X4Cc (Fig. 6D) ------- COMMENT: 50980b7b866ebd46 33 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: 50980b7b866ebd46 34 TGis3rUCEtmGm8asFYELUsr22YA (Fig. 6D and Fig. 7A) ------- COMMENT: 50980b7b866ebd46 35 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: 50980b7b866ebd46 36 vGtIAlSSPNnMrmPTCV9WpCBeLHE (Fig. 7B) ------- COMMENT: 50980b7b866ebd46 37 vGtIAlSSPNnMrmPTCV9WpCBeLHE (Fig. 7B) ------- COMMENT: 50980b7b866ebd46 38 vGtIAlSSPNnMrmPTCV9WpCBeLHE (Fig. 7B) ------- COMMENT: 50980b7b866ebd46 39 vGtIAlSSPNnMrmPTCV9WpCBeLHE (Fig. 7B) ------- COMMENT: 50980b7b866ebd46 40 kb7NOBRiqlmzY3vQrPFeR5NkglI (Fig. 7C) ------- COMMENT: 50980b7b866ebd46 41 kb7NOBRiqlmzY3vQrPFeR5NkglI (Fig. 7C) ------- COMMENT: 50980b7b866ebd46 42 kb7NOBRiqlmzY3vQrPFeR5NkglI (Fig. 7C) ------- COMMENT: 50980b7b866ebd46 43 kb7NOBRiqlmzY3vQrPFeR5NkglI (Fig. 7C) ------- COMMENT: 50980b7b866ebd46 44 kb7NOBRiqlmzY3vQrPFeR5NkglI (Fig. 7C) ------- COMMENT: 50980b7b866ebd46 46 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: 50980b7b866ebd46 47 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: 50980b7b866ebd46 48 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 50980b7b866ebd46 49 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 50980b7b866ebd46 50 hPFNK9pXn9s6uyKJPTkg3DIM2XE (Fig. 6A) ------- COMMENT: 50980b7b866ebd46 51 XwjFKIBpFnAfCL+3uv8ZHt+X4Cc (Fig. 6D) ------- COMMENT: 50980b7b866ebd46 52 XwjFKIBpFnAfCL+3uv8ZHt+X4Cc (Fig. 6D) ------- COMMENT: 50980b7b866ebd46 53 XwjFKIBpFnAfCL+3uv8ZHt+X4Cc (Fig. 6D) ------- COMMENT: 50980b7b866ebd46 54 XwjFKIBpFnAfCL+3uv8ZHt+X4Cc (Fig. 6D) ------- COMMENT: 50980b7b866ebd46 55 XwjFKIBpFnAfCL+3uv8ZHt+X4Cc (Fig. 6D) ------- COMMENT: 50980b7b866ebd46 56 XwjFKIBpFnAfCL+3uv8ZHt+X4Cc (Fig. 6D) ------- COMMENT: 509fee002381b763 4 jsSpqhMIP+Ev9zYRZ7YcaC6QhK0 fig2 (comment: likey due to intron encoded maturase) ------- COMMENT: 509fee002381b763 5 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 509fee002381b763 6 HTyLedbuHNi2PwOwrT9oBR8fxP0 fig3 (comment: CHECK abolished) ------- COMMENT: 509fee002381b763 7 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 509fee002381b763 8 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 509fee002381b763 9 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 509fee002381b763 10 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 509fee002381b763 11 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: 509fee002381b763 12 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 509fee002381b763 13 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 509fee002381b763 14 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 509fee002381b763 15 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 509fee002381b763 16 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 509fee002381b763 17 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 509fee002381b763 18 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 50a71e0348432087 3 aIliaf6592VCez+H/2TVpQhOVkY (comment: cdc2-F15 gene is expressed from episomal pIRT2) ------- COMMENT: 50a71e0348432087 4 aIliaf6592VCez+H/2TVpQhOVkY (comment: cdc2-F15 gene is expressed from episomal pIRT2) ------- COMMENT: 50a71e0348432087 6 M7B2gyyhjhWFMvzX/rq+ff1nG98 (comment: cdc25 over expressed from the constitutive ADH promoter. Data not shown) ------- COMMENT: 50a71e0348432087 7 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 50a71e0348432087 8 MEfQGKz5CSWD05jCGVTzr0mp7/o Figure 4D ------- COMMENT: 50a71e0348432087 9 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 50a71e0348432087 10 MEfQGKz5CSWD05jCGVTzr0mp7/o Figure 4D ------- COMMENT: 50a71e0348432087 11 46lY+MhXZ1zhQULP22V5QRmHO58 (comment: CHECK I'm sure this has already been annotated. But previous annotations didn't come up, should they?) ------- COMMENT: 50a71e0348432087 13 RUqgbx3TUqU4uRUnebSKIwZAiTM (comment: CHECK Also think this is previously annotated) ------- COMMENT: 50a71e0348432087 14 MEfQGKz5CSWD05jCGVTzr0mp7/o Figure 4D ------- COMMENT: 50a71e0348432087 16 silw5nL983EKbtU4U3cUwK7IL5Y Figure 4 ------- COMMENT: 50a71e0348432087 19 aIliaf6592VCez+H/2TVpQhOVkY cdc2-F15 (comment: gene is expressed from episomal pIRT2) ------- COMMENT: 50a71e0348432087 20 aIliaf6592VCez+H/2TVpQhOVkY cdc2-F15 (comment: gene is expressed from episomal pIRT2) ------- COMMENT: 50ae65179f9a18da 1 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: 50ae65179f9a18da 2 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: 50ae65179f9a18da 3 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 50ae65179f9a18da 4 pnEAMbbgorFLCc9tSrzgAxifNlg figure 1E ------- COMMENT: 50ae65179f9a18da 5 pnEAMbbgorFLCc9tSrzgAxifNlg figure 1E ------- COMMENT: 50ae65179f9a18da 6 BcMypYvwaLSjaZS/r7aYmOXmwYw figure2 ------- COMMENT: 50ae65179f9a18da 7 BcMypYvwaLSjaZS/r7aYmOXmwYw figure2 ------- COMMENT: 50ae65179f9a18da 9 GGgNkemktFQsu3SfpWZxXeVRkII figure 2a abolished ------- COMMENT: 50ae65179f9a18da 10 GGgNkemktFQsu3SfpWZxXeVRkII figure 2a (comment: CHECK abolished) ------- COMMENT: 50ae65179f9a18da 11 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 50db88fdd925afa8 1 iv56DZUhHEYiDt9wgwLvashPAU0 Cdc7-HA was not localized to SPBs during interphase (Fig. 1C, 1),, localized to both SPBs in metaphase and early anaphase cells (Fig. 1C, 2 and 3), and localized to one SPB in late mitotic cells (Fig. 1C, 4 – 6) (8). ------- COMMENT: 50db88fdd925afa8 2 QRn5xITJJfQ2BDAZuCydDKQdGhw Cdc7-HA was not localized to SPBs during interphase (Fig. 1C, 1), ------- COMMENT: 50db88fdd925afa8 4 2T7vW7AoJWA6EuU0lVgjyNcgTzI Spg1-HAH localized to SPBs throughout the cell cycle (Fig. 1B, second column) (8). Byr4 colocalized with Spg1-HAH during interphase (Fig. 1B, 1), but was absent from SPBs in metaphase (Fig. 1B, 2) and early anaphase ------- COMMENT: 50db88fdd925afa8 7 RkASr7DrWLTYZzMVYoQ8YffMNIU Later in anaphase, Byr4 colocalized with one SPB (Fig. 1B, 4). Byr4 colocalized with one or both SPBs in binucleate cells with septa (Fig. 1B, 5 and 6). In the vast majority of cells, Byr4 localized to SPBs that did not contain Cdc7 (Fig. 1C, 1, 4, and 5). ------- COMMENT: 50db88fdd925afa8 8 TBz5967o/CbbYh1tsY77lJ6sC+Q (comment: CHECK is this the right term?) ------- COMMENT: 50db88fdd925afa8 9 UhKmH6rjlZFvyTJOjVMI7WYzsfo (comment: CHECK combine, other binucleates should unde new term) ------- COMMENT: 50db88fdd925afa8 11 YMOWfc2FkYM+Jp/14hyw3Exfft0 This analysis of microtubule structures confirmed that mononucleate cells with Cdc7 local- ized to SPBs were in interphase and suggested that Byr4 was required to prevent septation during interphase. ------- COMMENT: 50db88fdd925afa8 12 nx0yFAZSiKK1Rw0YVOw56S4jBg8 Figure 2E. These results show that Byr4 is re- quired to prevent septation in G1 cells. ------- COMMENT: 50db88fdd925afa8 13 bEfkMXgbPr1bTToZisODyNkRWzY A corresponding decrease in the fraction of cells with Spg1 localized to SPBs occurred and reached 7% at 16 h (Fig. 3) ------- COMMENT: 50db88fdd925afa8 15 yX7gomT6A1z1svjWK0zYumulgCk As expected, most sid3–106 mutant cells (71%) contained four or more nuclei showing that there was insufficient Spg1 function in these cells for septation. ------- COMMENT: 50db88fdd925afa8 16 hc05HJ1YkPWGYPmlnFvSKBQJTno Examination of these cells following 12 h of growth in thiamine-containing medium also showed that most cells (59%) contained Byr4 at all SPBs (Table II). In contrast to sid3–106 mutants, though, the amount of Byr4 localized to SPBs in cells depleted of Spg1 using the conditional promoter was greatly reduced (data not shown) ------- COMMENT: 50db88fdd925afa8 17 0V3/avYGGZXmZdFiCNZkROVykQw Western analysis showed that the reduction in Byr4 localization to SPBs in cells depleted of Spg1 was not due to reduced Byr4 protein amounts (data not shown). ------- COMMENT: 50db88fdd925afa8 20 hc05HJ1YkPWGYPmlnFvSKBQJTno Examination of these cells following 12 h of growth in thiamine-containing medium also showed that most cells (59%) contained Byr4 at all SPBs (Table II). In contrast to sid3–106 mutants, though, the amount of Byr4 localized to SPBs in cells depleted of Spg1 using the conditional promoter was greatly reduced (data not shown) ------- COMMENT: 50db88fdd925afa8 21 wbZxCEt5dwv6JPQD7C7G3hp8kZk Western analysis showed that this decrease was not due to reduced Byr4 amounts ------- COMMENT: 50dfb504a775bd4d 4 5x5Slx/ivkZxidYPEawD7NlGfpU In sar1-1 cells, acid phosphatase accumulated in its 72 kDa core glycosylated form (arrow) even at 25°C (Fig. 2A, lane 5), and this form increased in abundance upon incubation at 36°C (Fig. 2A, lanes 6-8), indicating a block in its secretion from the ER ------- COMMENT: 50dfb504a775bd4d 5 wPPjUh0oR+jHi59QTm+GcbIT720 The glycosylation profile of acid phosphatase in sec31-1 cells also revealed a block in secretion from the ER (Fig. 2B). In sec31-1 cells at 25°C (Fig. 2B, lane 3), a low level of the 72 kDa form of acid phosphatase (arrow) and high molecular weight smears indicated normal protein secretion. However, sec31-1 cells incubated at 36°C for 4 hours, accumulated a high level of the 72 kDa ER form of acid phosphatase (Fig. 2B, lane 4), indicating that protein secretion from the ER is inhibited ------- COMMENT: 50dfb504a775bd4d 6 7PAf7w/PUZyODsTXrEV2BRCbCxw sar1, sec31 and pmm1 are essential genes ------- COMMENT: 50dfb504a775bd4d 7 7PAf7w/PUZyODsTXrEV2BRCbCxw sar1, sec31 and pmm1 are essential genes ------- COMMENT: 50dfb504a775bd4d 8 7PAf7w/PUZyODsTXrEV2BRCbCxw sar1, sec31 and pmm1 are essential genes ------- COMMENT: 50dfb504a775bd4d 9 7YInDAf1UVGs3wVGVZ8tsYHKu+g sar1, sec31 and pmm1 are essential genes vw:2c binucleate? should this be WT knockdown? ------- COMMENT: 50dfb504a775bd4d 12 6Yzhhc6UjRm8qGXmuPdSyEqnTdw These data confirm that pmm1 is an essential gene. Microscopic examination of the tetrad dissection plates revealed that, of 54 pmm1 null spores examined, 98% germinated. Of these, 52% formed single, rounded cells and 47% arrested as single, septated cells. ------- COMMENT: 50dfb504a775bd4d 13 CVT0WxU8kxHl88ZvUEYLjMtZWs0 19% were septated, binucleated cells with condensed chromosomes. (comment: vw:2c binucleate? should this be WT knockdown?) ------- COMMENT: 50dfb504a775bd4d 14 y6aQbbT2mq82jU8TL6RqVL8J61U 56% of sar1-1, 68% of sec31-1, and 50% of pmm1-1 cells had accumulated ER membranes and dilated nuclear and ER lumens (Table 1). ------- COMMENT: 50dfb504a775bd4d 15 y6aQbbT2mq82jU8TL6RqVL8J61U 56% of sar1-1, 68% of sec31-1, and 50% of pmm1-1 cells had accumulated ER membranes and dilated nuclear and ER lumens (Table 1). ------- COMMENT: 50dfb504a775bd4d 16 y6aQbbT2mq82jU8TL6RqVL8J61U 56% of sar1-1, 68% of sec31-1, and 50% of pmm1-1 cells had accumulated ER membranes and dilated nuclear and ER lumens (Table 1). ------- COMMENT: 50dfb504a775bd4d 17 y6aQbbT2mq82jU8TL6RqVL8J61U 56% of sar1-1, 68% of sec31-1, and 50% of pmm1-1 cells had accumulated ER membranes and dilated nuclear and ER lumens (Table 1). ------- COMMENT: 50dfb504a775bd4d 18 y6aQbbT2mq82jU8TL6RqVL8J61U 56% of sar1-1, 68% of sec31-1, and 50% of pmm1-1 cells had accumulated ER membranes and dilated nuclear and ER lumens (Table 1). ------- COMMENT: 50dfb504a775bd4d 19 3RHA/PNBD10ae5SXPpamKdKGOys Pap1p was localized predominantly to the cytoplasm in wild- type cells (Fig. 5A) as well as in the sar1-1, sec31-1 and pmm1- 1 mutants grown at the restrictive temperature, suggesting that nuclear protein export was not adversely affected in these cells (Fig. 5C,E,G). ------- COMMENT: 50dfb504a775bd4d 20 3RHA/PNBD10ae5SXPpamKdKGOys Pap1p was localized predominantly to the cytoplasm in wild- type cells (Fig. 5A) as well as in the sar1-1, sec31-1 and pmm1- 1 mutants grown at the restrictive temperature, suggesting that nuclear protein export was not adversely affected in these cells (Fig. 5C,E,G). ------- COMMENT: 50dfb504a775bd4d 21 3RHA/PNBD10ae5SXPpamKdKGOys Pap1p was localized predominantly to the cytoplasm in wild- type cells (Fig. 5A) as well as in the sar1-1, sec31-1 and pmm1- 1 mutants grown at the restrictive temperature, suggesting that nuclear protein export was not adversely affected in these cells (Fig. 5C,E,G). ------- COMMENT: 50dfb504a775bd4d 22 3RHA/PNBD10ae5SXPpamKdKGOys Pap1p was localized predominantly to the cytoplasm in wild- type cells (Fig. 5A) as well as in the sar1-1, sec31-1 and pmm1- 1 mutants grown at the restrictive temperature, suggesting that nuclear protein export was not adversely affected in these cells (Fig. 5C,E,G). ------- COMMENT: 50dfb504a775bd4d 23 3RHA/PNBD10ae5SXPpamKdKGOys Pap1p was localized predominantly to the cytoplasm in wild- type cells (Fig. 5A) as well as in the sar1-1, sec31-1 and pmm1- 1 mutants grown at the restrictive temperature, suggesting that nuclear protein export was not adversely affected in these cells (Fig. 5C,E,G). ------- COMMENT: 50dfb504a775bd4d 24 3RHA/PNBD10ae5SXPpamKdKGOys Pap1p was localized predominantly to the cytoplasm in wild- type cells (Fig. 5A) as well as in the sar1-1, sec31-1 and pmm1- 1 mutants grown at the restrictive temperature, suggesting that nuclear protein export was not adversely affected in these cells (Fig. 5C,E,G). ------- COMMENT: 50dfb504a775bd4d 34 y6aQbbT2mq82jU8TL6RqVL8J61U 56% of sar1-1, 68% of sec31-1, and 50% of pmm1-1 cells had accumulated ER membranes and dilated nuclear and ER lumens (Table 1). ------- COMMENT: 50dfb504a775bd4d 35 y6aQbbT2mq82jU8TL6RqVL8J61U 56% of sar1-1, 68% of sec31-1, and 50% of pmm1-1 cells had accumulated ER membranes and dilated nuclear and ER lumens (Table 1). ------- COMMENT: 50dfb504a775bd4d 36 y6aQbbT2mq82jU8TL6RqVL8J61U 56% of sar1-1, 68% of sec31-1, and 50% of pmm1-1 cells had accumulated ER membranes and dilated nuclear and ER lumens (Table 1). ------- COMMENT: 50dfb504a775bd4d 37 y6aQbbT2mq82jU8TL6RqVL8J61U 56% of sar1-1, 68% of sec31-1, and 50% of pmm1-1 cells had accumulated ER membranes and dilated nuclear and ER lumens (Table 1). ------- COMMENT: 50f2e165a05393f0 8 46Nf2LL8v+49jBvXBBiXsnvha7E fig 5d,e. Only in presence of cid14/16 does rrp6 degrade ago1 bound rnas. This mechanism protects the genome from uncontrolled small RNAs ------- COMMENT: 50f2e165a05393f0 10 +pZ3pBIDULWBpWmjA1dTrzT8WdY (comment: CHECK see comment on cid14) ------- COMMENT: 515169773959f90f 2 zf6MfaVw0ziX9S+kDQ/qChHYVqg fig 1A (comment: CHECK inviable) ------- COMMENT: 515169773959f90f 7 ksQ8KGx/c8HbUbBKbwB9bIl/Wfs fig 2, 3 ------- COMMENT: 515169773959f90f 8 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: 515169773959f90f 9 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: 515169773959f90f 10 crdk8+5Su6ZjOoDfvYBv9bmPLs0 Figure 5C ------- COMMENT: 515169773959f90f 11 C9DfreRzxECuv4dR8XA4y5vKLo8 (comment: UPR) ------- COMMENT: 515169773959f90f 12 C9DfreRzxECuv4dR8XA4y5vKLo8 (comment: UPR) ------- COMMENT: 515169773959f90f 13 C9DfreRzxECuv4dR8XA4y5vKLo8 (comment: UPR) ------- COMMENT: 515169773959f90f 29 ksQ8KGx/c8HbUbBKbwB9bIl/Wfs fig 2, 3 ------- COMMENT: 51ab1cf2ef51756d 13 lOxuMeVQtusDsvtXf0LEKsgc+1M (comment: CHECK get double mutant phenotypes) ------- COMMENT: 51afeaedc270a24c 37 vmOMawZCAry1CcMYTVX/QAIV8u0 Figure 6A ------- COMMENT: 51afeaedc270a24c 40 LkHNgkt5VUCmgtb/uSzxovwJr78 Figure 7c ------- COMMENT: 51ea31a1f9fbdb80 7 ewfspod7Bin5Lx5/B/NV8EarpfI (comment: CONDITION 30 degrees) ------- COMMENT: 51ea31a1f9fbdb80 12 ewfspod7Bin5Lx5/B/NV8EarpfI (comment: CONDITION 30 degrees) ------- COMMENT: 51ea31a1f9fbdb80 13 ewfspod7Bin5Lx5/B/NV8EarpfI (comment: CONDITION 30 degrees) ------- COMMENT: 51ea31a1f9fbdb80 14 ewfspod7Bin5Lx5/B/NV8EarpfI (comment: CONDITION 30 degrees) ------- COMMENT: 51ea31a1f9fbdb80 15 ewfspod7Bin5Lx5/B/NV8EarpfI (comment: CONDITION 30 degrees) ------- COMMENT: 51ea31a1f9fbdb80 16 ewfspod7Bin5Lx5/B/NV8EarpfI (comment: CONDITION 30 degrees) ------- COMMENT: 51ea31a1f9fbdb80 19 ewfspod7Bin5Lx5/B/NV8EarpfI (comment: CONDITION 30 degrees) ------- COMMENT: 51ea31a1f9fbdb80 22 ewfspod7Bin5Lx5/B/NV8EarpfI (comment: CONDITION 30 degrees) ------- COMMENT: 51ea31a1f9fbdb80 35 ewfspod7Bin5Lx5/B/NV8EarpfI (comment: CONDITION 30 degrees) ------- COMMENT: 51ea31a1f9fbdb80 36 ewfspod7Bin5Lx5/B/NV8EarpfI (comment: CONDITION 30 degrees) ------- COMMENT: 51fe04707302115f 1 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: 51fe04707302115f 3 77tbnZFrYej6ccuV28mbEQ524Z4 These data indicate that the localization of the Crs1-GFP cyclin during meiosis is similar to that of a SPB component. (Fig. 5) ------- COMMENT: 51fe04707302115f 4 QMrXTC/mCqLBo3Pef7y+iFuHtKE (Fig. 4 and Fig. S2) ------- COMMENT: 51fe04707302115f 5 QMrXTC/mCqLBo3Pef7y+iFuHtKE (Fig. 4 and Fig. S2) ------- COMMENT: 51fe04707302115f 6 QMrXTC/mCqLBo3Pef7y+iFuHtKE (Fig. 4 and Fig. S2) ------- COMMENT: 51fe04707302115f 7 tpbfizeW86+r0KQON0uJtosMz/c In some nuclei, a diffuse pan-nuclear signal was also detected. (Fig. 5) ------- COMMENT: 51fe04707302115f 8 +cE/sndqxzdjGCnPqUrorrxMvsc (Fig. 7, Fig. S4 and Fig. S5) ------- COMMENT: 51fe04707302115f 9 +cE/sndqxzdjGCnPqUrorrxMvsc (Fig. 7, Fig. S4 and Fig. S5) ------- COMMENT: 51fe04707302115f 11 TQswKTQbVy4BmacYDTi4A/UGKgI (Fig. 8) ------- COMMENT: 51fe04707302115f 12 OQvNobiRAoWhNT+sGEFlnJ2nXtM (Fig. S6) ------- COMMENT: 51fe04707302115f 13 h+6+K9FJl9bD96Qva54uF+a+gDc Crs1 showed associated kinase activity that was temperature sensitive and Cdc2-dependent (Figure 2b). ------- COMMENT: 51fe04707302115f 15 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: 51fe04707302115f 16 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: 51fe04707302115f 17 TQswKTQbVy4BmacYDTi4A/UGKgI (Fig. 8) ------- COMMENT: 5219d3ee134bc21e 3 kVzDHk2nsKkUGrPsDuF/PG17/ls (comment: it doesn't say old, but it is...) ------- COMMENT: 5219d3ee134bc21e 8 kVzDHk2nsKkUGrPsDuF/PG17/ls (comment: it doesn't say old, but it is...) ------- COMMENT: 522133ffc88d3f1f 7 bQrAvw5v3pdeGEjTasNZSNQl2dw Figure 6A ------- COMMENT: 522133ffc88d3f1f 8 bQrAvw5v3pdeGEjTasNZSNQl2dw Figure 6A ------- COMMENT: 522133ffc88d3f1f 10 bQrAvw5v3pdeGEjTasNZSNQl2dw Figure 6A ------- COMMENT: 522133ffc88d3f1f 11 vcr+7qtELpi85citxIC3W+pDl8I Figure 6B ------- COMMENT: 5225c988b7cbf341 2 ykoNlgywiSJuKbdm8ZOCk8eUvqw fig 1b The estimated doubling time of the mutant was 110 min in a rich (YE) medium, while that of the wild-type cells was 150 min. ------- COMMENT: 5225c988b7cbf341 3 KvCNuCWxBa0MFcYCZ5iUshqia3w small daughter at g1 Fig 1c The mutant produced daughter cells with an average length of 6 μm; whereas, the wild-type cells averaged 7.5 μm ------- COMMENT: 5225c988b7cbf341 4 hre1VlEHWHP9IFYCK0U0bjP8bPU Fig 1c The mutant produced daughter cells with an average length of 6 μm; whereas, the wild-type cells averaged 7.5 μm ------- COMMENT: 5225c988b7cbf341 5 woLeZfQg6Q17IIo6Yy7lmCNie6A As shown in Fig. 2A, the rate of cell proliferation was immediately reduced after the amount of Rrg1 was increased. ------- COMMENT: 5225c988b7cbf341 6 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 5225c988b7cbf341 7 TbKcGDbP/fkoPPuH8R5yD6dL5eM (Figs. 3A and 3B). there were cells that initiated and completed the mitosis in a small portion (Table 1), which eventually led to cell proliferation until the stationary phase (Fig. 2A). ------- COMMENT: 5225c988b7cbf341 8 kZ7GVsaZuEXeBXuD20QOPluJeLo (Figs. 3A and 3B). ------- COMMENT: 5225c988b7cbf341 9 soK3rnETKH2ekj2iCc+lwFy5ljo a considerable portion of the Rrg1-overproduced cells that undergo mitosis showed an abnormal accumulation of septum material (Figs. 3F, 3G, and 3H). ------- COMMENT: 5251feea6fc8d5fc 2 pzXo3ht+1afWENuKdmC1+zJo+2I (comment: CHECK -regulation - can also infer from GO:0030674) ------- COMMENT: 5251feea6fc8d5fc 6 cP4cY4g3p9DoPH2q/4G3XT8gI58 (comment: same as csn1delta alone) ------- COMMENT: 5251feea6fc8d5fc 9 Yk+N6y0tYNhc5oRKyhrydVCzy04 (comment: same as cdt2delta alone) ------- COMMENT: 5251feea6fc8d5fc 10 u8UaAK+THULtIpZGOBq35q7RlNY (comment: same as ddb1delta alone) ------- COMMENT: 5251feea6fc8d5fc 11 Yk+N6y0tYNhc5oRKyhrydVCzy04 (comment: same as cdt2delta alone) ------- COMMENT: 5251feea6fc8d5fc 12 u8UaAK+THULtIpZGOBq35q7RlNY (comment: same as ddb1delta alone) ------- COMMENT: 5251feea6fc8d5fc 15 ds/Q968PiXsW6esye5h15HFudjo (comment: same as pcu4delta alone) ------- COMMENT: 5251feea6fc8d5fc 16 ds/Q968PiXsW6esye5h15HFudjo (comment: same as pcu4delta alone_ ------- COMMENT: 5254065d0b16e9e5 4 sWB5eUT4u7fFEDFgHNaNrXiaN5M Importantly, cells lacking only the Chp1 chromodo- main (CDchp1-6xmyc) behaved similar to the chp1 null strain, with elevated transcription of the centromeric marker gene (Fig. 2C) and numerous mitotic chromosome segregation de- fects (Fig. 2D) ------- COMMENT: 5254065d0b16e9e5 5 sWB5eUT4u7fFEDFgHNaNrXiaN5M Importantly, cells lacking only the Chp1 chromodo- main (CDchp1-6xmyc) behaved similar to the chp1 null strain, with elevated transcription of the centromeric marker gene (Fig. 2C) and numerous mitotic chromosome segregation de- fects (Fig. 2D) ------- COMMENT: 5254065d0b16e9e5 6 Dd2mnTE+QSaUF/HMVKj8x0+wU48 n contrast, cells lacking the RRM domain of Chp1 (RRMchp1-6xmyc) exhibited no loss of Chp1 function. ------- COMMENT: 5254065d0b16e9e5 7 Dd2mnTE+QSaUF/HMVKj8x0+wU48 n contrast, cells lacking the RRM domain of Chp1 (RRMchp1-6xmyc) exhibited no loss of Chp1 function. ------- COMMENT: 5254065d0b16e9e5 8 u2V4OAQ/+7Z1WppFfayIg4J73Xk In contrast, CDchp1-6xmyc exhibited a diffuse faint spotty staining pattern throughout the nucleoplasm and was not as- sociated with chromatin (Fig. 2E). Thus, the chromodomain, but not the RRM, is essential for Chp1 localization to all sites of heterochromatin and for normal Chp1 function at centro- meric sequences. ------- COMMENT: 5254065d0b16e9e5 9 L2oTnFin6GHsnuT0CVPMLCnK1fQ These cells exhibited high levels of chromosome segregation defects (Fig. 3C) and defects in centromeric silencing (Fig. 3B). ------- COMMENT: 5254065d0b16e9e5 10 L2oTnFin6GHsnuT0CVPMLCnK1fQ These cells exhibited high levels of chromosome segregation defects (Fig. 3C) and defects in centromeric silencing (Fig. 3B). ------- COMMENT: 5254065d0b16e9e5 11 F3wPq975RatszRRKyQOZ9zYhVdM Consistent with this result, immunolocalization of 3xHA- chp11–409 with anti-HA antibody revealed that the truncated protein was diffusely localized throughout the chromatin and nucleolus (Fig. 3D). ------- COMMENT: 5254065d0b16e9e5 12 ZKnAQ2titXs+qw0fFD4COSQXkPU Thus, Tas3-13xmyc and Chp1-6xmyc colocalize to the mat2/3 locus, to telomeres, and to centromeres and possibly associate with all sites of heterochromatin. ------- COMMENT: 5254065d0b16e9e5 13 ZKnAQ2titXs+qw0fFD4COSQXkPU Thus, Tas3-13xmyc and Chp1-6xmyc colocalize to the mat2/3 locus, to telomeres, and to centromeres and possibly associate with all sites of heterochromatin. ------- COMMENT: 5254065d0b16e9e5 14 ZKnAQ2titXs+qw0fFD4COSQXkPU Thus, Tas3-13xmyc and Chp1-6xmyc colocalize to the mat2/3 locus, to telomeres, and to centromeres and possibly associate with all sites of heterochromatin. ------- COMMENT: 5254065d0b16e9e5 15 5dIU5qQdj5K0075ewoxG+/qM0Hc in ago1 cells there is a loss of centromere-associated Chp1 and Tas3, as revealed by loss of costaining of Chp1-6xmyc (Fig. 5A) and Tas3-13xmyc (Fig. 5B) ------- COMMENT: 5254065d0b16e9e5 16 5dIU5qQdj5K0075ewoxG+/qM0Hc in ago1 cells there is a loss of centromere-associated Chp1 and Tas3, as revealed by loss of costaining of Chp1-6xmyc (Fig. 5A) and Tas3-13xmyc (Fig. 5B) ------- COMMENT: 5254065d0b16e9e5 17 kbhxLRhUP+QCnU1i70QVFYkK1kI Surprisingly, however, other foci of Chp1-6xmyc and Tas3- 13xmyc persisted in ago1 cells. Similar results were obtained in cells from which Dicer was deleted (Fig. 5A and B, right panels). ------- COMMENT: 5254065d0b16e9e5 18 kbhxLRhUP+QCnU1i70QVFYkK1kI Surprisingly, however, other foci of Chp1-6xmyc and Tas3- 13xmyc persisted in ago1 cells. Similar results were obtained in cells from which Dicer was deleted (Fig. 5A and B, right panels). ------- COMMENT: 5254065d0b16e9e5 19 kbhxLRhUP+QCnU1i70QVFYkK1kI Surprisingly, however, other foci of Chp1-6xmyc and Tas3- 13xmyc persisted in ago1 cells. Similar results were obtained in cells from which Dicer was deleted (Fig. 5A and B, right panels). ------- COMMENT: 5254065d0b16e9e5 20 kbhxLRhUP+QCnU1i70QVFYkK1kI Surprisingly, however, other foci of Chp1-6xmyc and Tas3- 13xmyc persisted in ago1 cells. Similar results were obtained in cells from which Dicer was deleted (Fig. 5A and B, right panels). ------- COMMENT: 5254065d0b16e9e5 21 aQyjH1GyokHtxyn3oRMBCsl6n7Q We also assessed the localization of Chp1-6xmyc and Tas3- 13xmyc in cells lacking tas3 and chp1, respectively (Fig. 6A). In cells with tas3 deleted, Chp1 was delocalized, with a cloud of small foci of staining that mainly accumulated over the nucle- olus (Fig. 6A) ------- COMMENT: 5254065d0b16e9e5 22 TfclLHJF2ntjIvDGvbgX2GI1dho Tas3-13xmyc protein was essentially lost in chp1 cells, whereas it was present at normal levels in the ago1 cells (Fig. 6B) To address whether loss of Tas3-13xmyc protein in chp1 cells was due to suppression of tas3-13xmyc transcription, we performed quantitative RT-PCR using RNA prepared from wild-type, chp1, and ago1 backgrounds. Deletion of chp1 had no effect on tas3-13xmyc transcript levels (Fig. 6C); therefore, the loss of Tas3 protein in chp1 null cells is a post- transcriptional effect. ------- COMMENT: 5254065d0b16e9e5 24 xVwKrARkf0d8VI1+60gxgYAiZO4 Nonetheless, deletion of chp1 or ago1 only slightly alleviated silencing (20, 38), as revealed by the presence of pink colonies compared with the red, fully repressed colonies (Fig. 8A). ------- COMMENT: 5254065d0b16e9e5 25 xVwKrARkf0d8VI1+60gxgYAiZO4 Nonetheless, deletion of chp1 or ago1 only slightly alleviated silencing (20, 38), as revealed by the presence of pink colonies compared with the red, fully repressed colonies (Fig. 8A). ------- COMMENT: 526628f82b16fbfd 10 rQCFThA2HPuBnxDA4fMTRiaO+gs (comment: same as taz1delta alone) ------- COMMENT: 526628f82b16fbfd 16 rQCFThA2HPuBnxDA4fMTRiaO+gs (comment: same as taz1delta alone) ------- COMMENT: 527b6a62edc40016 1 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 2 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 3 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 5 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 6 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 7 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 8 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 9 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 10 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 11 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 12 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 13 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 14 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 15 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 16 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 17 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 18 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 19 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 20 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 21 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 22 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 23 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 24 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 25 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 26 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 27 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 28 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 29 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 30 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 31 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 32 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 33 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 34 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 35 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 36 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 37 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 38 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 39 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 40 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 41 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 42 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 43 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 44 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 45 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 46 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 47 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 48 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 49 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 50 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 51 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 52 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 53 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 54 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 55 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 56 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 57 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 58 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 59 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 60 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 61 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 62 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 527b6a62edc40016 63 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 527b6a62edc40016 64 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 527b6a62edc40016 65 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 527b6a62edc40016 66 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 527b6a62edc40016 67 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 527b6a62edc40016 68 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 527b6a62edc40016 69 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 527b6a62edc40016 70 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 71 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 527b6a62edc40016 72 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 527b6a62edc40016 73 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 527b6a62edc40016 74 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 527b6a62edc40016 75 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 527b6a62edc40016 76 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 527b6a62edc40016 77 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 527b6a62edc40016 78 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 527b6a62edc40016 79 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 527b6a62edc40016 80 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 527b6a62edc40016 81 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 527b6a62edc40016 82 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 527b6a62edc40016 83 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 527b6a62edc40016 84 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 527b6a62edc40016 85 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 527b6a62edc40016 86 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 527b6a62edc40016 87 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 527b6a62edc40016 88 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 89 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 90 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 91 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 527b6a62edc40016 92 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 527b6a62edc40016 93 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 527b6a62edc40016 94 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 95 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 96 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 97 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 98 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 99 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 100 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 101 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 102 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 103 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 104 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 105 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 106 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 107 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 108 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 109 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 110 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 111 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 112 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 113 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 114 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 115 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 116 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 117 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 118 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 119 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 120 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 121 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 122 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 123 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 124 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 527b6a62edc40016 125 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 527b6a62edc40016 126 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 527b6a62edc40016 127 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 527b6a62edc40016 128 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 527b6a62edc40016 129 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 527b6a62edc40016 130 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 131 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 132 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 133 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 134 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 135 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 136 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 137 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 138 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 139 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 140 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 141 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 142 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 143 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 144 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 145 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 146 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 147 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 148 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 527b6a62edc40016 149 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 527b6a62edc40016 150 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 527b6a62edc40016 151 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 527b6a62edc40016 152 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 527b6a62edc40016 153 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 527b6a62edc40016 154 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 527b6a62edc40016 155 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 527b6a62edc40016 156 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 527b6a62edc40016 157 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 527b6a62edc40016 158 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 159 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 160 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 161 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 162 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 163 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 527b6a62edc40016 164 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 527b6a62edc40016 165 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 527b6a62edc40016 166 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 527b6a62edc40016 167 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 527b6a62edc40016 168 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 527b6a62edc40016 169 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 527b6a62edc40016 170 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 527b6a62edc40016 171 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 527b6a62edc40016 172 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 527b6a62edc40016 173 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 527b6a62edc40016 174 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 175 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 176 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 177 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 178 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 179 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 180 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 181 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 182 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 183 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 184 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 185 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 186 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 187 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 188 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 189 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 190 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 527b6a62edc40016 191 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 52a35f8f6a41d529 1 kGDl9zMn3GJ8dOBcEDTxm2IW+Ow derepression of a ura4+ marker gene integrated into the outermost Fig. 1. (otr) pericentromeric repeat of chromosome 1 (otr1R::ura4+) (Fig. 1a) ------- COMMENT: 52a35f8f6a41d529 2 Rjn3ewTIGGCTmH9ZOpKf/ygc2Xs increased levels of noncoding centromeric transcripts (Fig. 1B) ------- COMMENT: 52a35f8f6a41d529 3 EzebtLulaO69lR4tDvcjXS8xD+M defective siRNA production (Fig. 1C), ------- COMMENT: 52a35f8f6a41d529 4 kGDl9zMn3GJ8dOBcEDTxm2IW+Ow derepression of a ura4+ marker gene integrated into the outermost Fig. 1. (otr) pericentromeric repeat of chromosome 1 (otr1R::ura4+) (Fig. 1a) ------- COMMENT: 52a35f8f6a41d529 5 kGDl9zMn3GJ8dOBcEDTxm2IW+Ow derepression of a ura4+ marker gene integrated into the outermost Fig. 1. (otr) pericentromeric repeat of chromosome 1 (otr1R::ura4+) (Fig. 1a) ------- COMMENT: 52a35f8f6a41d529 6 bG5L8bnCpFy/rFA2jRt26FuYhq0 Similar to that previously observed, EGFP-Ers1WT showed a distinct nuclear dot pattern in WT cells consistent with a locali- zation to heterochromatin (Fig. 4B and Fig. S4B) ------- COMMENT: 52a35f8f6a41d529 7 65VLjkvGgwmuRaCKhNEmOnxI2DU In contrast, EGFP-Ers1C62 showed a more diffuse signal with weak nuclear dots (Fig. 4B and Fig. S4 C and D), suggesting that the correct localization of Ers1 was impaired by the C62 mutation. ------- COMMENT: 52a35f8f6a41d529 8 VGZeXp10a/LPeQNZfUjE6WfvqIc Moreover, the nuclear dot localization of EGFP-Ers1WT was completely abolished in clr4Δ cells (Fig. 4C), indicating that the activity of Clr4 was also required for Ers1 localization in the nucleus. ------- COMMENT: 52a35f8f6a41d529 9 iZmnC/k5kfB7E7mD2Hjrz7OAAPs To test the hypothesis that the nu- clear dot localization of Ers1 was attributable to an interaction with H3K9me-bound CD proteins, EGFP-Ers1WT localization was next examined in swi6Δ, chp1Δ, and chp2Δ cells. The localization of EGFP-Ers1WT was clearly abolished in swi6Δ cells similar to that observed in clr4Δ, whereas WT localization patterns were retained in the chp1Δ and chp2Δ cells (Fig. 4C and Fig. S4C). ------- COMMENT: 52a35f8f6a41d529 10 iZmnC/k5kfB7E7mD2Hjrz7OAAPs To test the hypothesis that the nu- clear dot localization of Ers1 was attributable to an interaction with H3K9me-bound CD proteins, EGFP-Ers1WT localization was next examined in swi6Δ, chp1Δ, and chp2Δ cells. The localization of EGFP-Ers1WT was clearly abolished in swi6Δ cells similar to that observed in clr4Δ, whereas WT localization patterns were retained in the chp1Δ and chp2Δ cells (Fig. 4C and Fig. S4C). ------- COMMENT: 52a35f8f6a41d529 11 iZmnC/k5kfB7E7mD2Hjrz7OAAPs To test the hypothesis that the nu- clear dot localization of Ers1 was attributable to an interaction with H3K9me-bound CD proteins, EGFP-Ers1WT localization was next examined in swi6Δ, chp1Δ, and chp2Δ cells. The localization of EGFP-Ers1WT was clearly abolished in swi6Δ cells similar to that observed in clr4Δ, whereas WT localization patterns were retained in the chp1Δ and chp2Δ cells (Fig. 4C and Fig. S4C). ------- COMMENT: 52a35f8f6a41d529 12 z7FyqCjb5g7WExGvDaHVB8iiJX0 sug- gesting that a physical association with Swi6, but not the other CD proteins, was required for the heterochromatic localization of Ers1. ------- COMMENT: 52a35f8f6a41d529 13 pN5UzyY6mqCVeQ/mBDgzKBgOVh0 Moreover, the interaction between Ers1 and Swi6 was weakened by the presence of the C62 mutation (Fig. 4 D and E). Taken together, these results suggested that Ers1 associates with both Hrr1 and Swi6, and that the C62 mutation impairs both associations. ------- COMMENT: 52a35f8f6a41d529 14 ONWcq3rGDJqk10ydcQ7bLdeIeIQ The localizations of Hrr1-Flag and Rdp1-Flag at centro- meric repeats were also found to be severely compromised in both ers1Δ and swi6Δ mutant cells (Fig. 5 A and B). ------- COMMENT: 52a35f8f6a41d529 15 ONWcq3rGDJqk10ydcQ7bLdeIeIQ The localizations of Hrr1-Flag and Rdp1-Flag at centro- meric repeats were also found to be severely compromised in both ers1Δ and swi6Δ mutant cells (Fig. 5 A and B). ------- COMMENT: 52a35f8f6a41d529 16 ONWcq3rGDJqk10ydcQ7bLdeIeIQ The localizations of Hrr1-Flag and Rdp1-Flag at centro- meric repeats were also found to be severely compromised in both ers1Δ and swi6Δ mutant cells (Fig. 5 A and B). ------- COMMENT: 52a35f8f6a41d529 17 ONWcq3rGDJqk10ydcQ7bLdeIeIQ The localizations of Hrr1-Flag and Rdp1-Flag at centro- meric repeats were also found to be severely compromised in both ers1Δ and swi6Δ mutant cells (Fig. 5 A and B). ------- COMMENT: 52a35f8f6a41d529 18 ohFD0FGDra5gjOB0o80MRHHxRNo This is also con- sistent with a previous report that the deletion of swi6+ decreases the centromeric localization of Rdp1 (14). These results support the idea that the heterochromatic localization of RDRC requires Ers1 and that, in turn, Ers1 localization depends on Swi6. ------- COMMENT: 52a35f8f6a41d529 19 jZNBMIVwse33Pf4aUthh92sHvnk ChIP analyses showed that Ers1 localization at the mating type locus (cenH) and telomeres was severely de- creased in swi6Δ and clr4Δ cells (Fig. S5A). ------- COMMENT: 52a35f8f6a41d529 20 jZNBMIVwse33Pf4aUthh92sHvnk ChIP analyses showed that Ers1 localization at the mating type locus (cenH) and telomeres was severely de- creased in swi6Δ and clr4Δ cells (Fig. S5A). ------- COMMENT: 52a35f8f6a41d529 21 jZNBMIVwse33Pf4aUthh92sHvnk ChIP analyses showed that Ers1 localization at the mating type locus (cenH) and telomeres was severely de- creased in swi6Δ and clr4Δ cells (Fig. S5A). ------- COMMENT: 52a35f8f6a41d529 22 jZNBMIVwse33Pf4aUthh92sHvnk ChIP analyses showed that Ers1 localization at the mating type locus (cenH) and telomeres was severely de- creased in swi6Δ and clr4Δ cells (Fig. S5A). ------- COMMENT: 52a35f8f6a41d529 23 /hb3GYzWsGiQjR9nDj3K8/TT3l8 In contrast, a high level of Ers1 was detected at telomeres in hrr1Δ cells (Fig. S5C). ------- COMMENT: 52a35f8f6a41d529 24 UK1slECjn39Nb/LUxVPF+z/0+mA In swi6Δ cells, Chp1 association with the centromeric dg or dh repeat locus decreased partially (70– 80%) but was still much greater than that observed in clr4Δ cells (Fig. 5 A and B). ------- COMMENT: 52a35f8f6a41d529 25 8dUu5TjGAHIhC34pcZQMPzViD8c This was in contrast to Hrr1 or Rdp1, whose association was severely reduced in swi6Δ cells (10–20%) to levels comparable to those of clr4Δ cells. ------- COMMENT: 52a35f8f6a41d529 26 8dUu5TjGAHIhC34pcZQMPzViD8c This was in contrast to Hrr1 or Rdp1, whose association was severely reduced in swi6Δ cells (10–20%) to levels comparable to those of clr4Δ cells. ------- COMMENT: 52d9059eb1a6c990 1 5mXFSQMSwf2nsGVUzRPnOc6N4eg (comment: Rad3 phosphorylates T11 in response to hydroxyurea treatment) ------- COMMENT: 52d9059eb1a6c990 13 JrHMkf9wW2gCDLBjSARaSOAP0DU (comment: CHECK cellular response to hydroxyurea) ------- COMMENT: 52d9059eb1a6c990 14 zfuRyxFEEpcLBA2Y4DTA4O6fuWg (comment: CHECK phosphorylates Cds1) ------- COMMENT: 52dd09ea98dec059 1 Ge4wjQtEhkC/56WEYNd/tZ7upjs Growth of the vps3844Δ strain was significantly inhibited at low temperature (Fig. 2B). ------- COMMENT: 52dd09ea98dec059 2 hAvR7txzQsYGN6NFbbNEAYOvExo To confirm that SPBC1709.03 is a VPS gene, extracellular leakage of CPY was assessed 17 by colony blot assay (Fig. 1C). Relative to wild type (WT), the SPBC1709.03 deletion strain 18 showed higher chemiluminescence intensity of CPY, indicating an increased occurrence of 19 CPY mis-sorting to the cell surface. ------- COMMENT: 52dd09ea98dec059 3 oxKj4yhrYKxbpOVrz9tVB9lb1SU 1 showed that the signal peptide and the transmembrane including DUF3844 domain were 2 essential for this function (Fig. 4A). ------- COMMENT: 52dd09ea98dec059 4 oxKj4yhrYKxbpOVrz9tVB9lb1SU 1 showed that the signal peptide and the transmembrane including DUF3844 domain were 2 essential for this function (Fig. 4A). ------- COMMENT: 52dd09ea98dec059 5 oxKj4yhrYKxbpOVrz9tVB9lb1SU 1 showed that the signal peptide and the transmembrane including DUF3844 domain were 2 essential for this function (Fig. 4A). ------- COMMENT: 52dd09ea98dec059 6 oxKj4yhrYKxbpOVrz9tVB9lb1SU 1 showed that the signal peptide and the transmembrane including DUF3844 domain were 2 essential for this function (Fig. 4A). ------- COMMENT: 52dd09ea98dec059 7 oxKj4yhrYKxbpOVrz9tVB9lb1SU 1 showed that the signal peptide and the transmembrane including DUF3844 domain were 2 essential for this function (Fig. 4A). ------- COMMENT: 52dd09ea98dec059 8 G/npqcalVd7XVZ3owcFw5f6G9T0 Although the Vps3844 protein is important for the transport of 26 CPY into the vacuole, Isp6-GFP and Psp3-GFP were found to localize in the vacuolar lumen 27 in vps3844Δ cells (Fig. S5). ------- COMMENT: 52dd09ea98dec059 9 G/npqcalVd7XVZ3owcFw5f6G9T0 Although the Vps3844 protein is important for the transport of 26 CPY into the vacuole, Isp6-GFP and Psp3-GFP were found to localize in the vacuolar lumen 27 in vps3844Δ cells (Fig. S5). ------- COMMENT: 52e0036cabf3303e 1 wVFnB8T8c0ZVLpkNxt2dbXyKkGo (comment: cdc18-1-141 when expressed on multi copy plasmid does not rescue cdc18-K46) ------- COMMENT: 52e0036cabf3303e 2 QTPKKLvVZQ0/Nqu3wSrTclLdYo4 (comment: cdc18-150-577 when expressed on multi copy plasmid does not rescue cdc18-K46) ------- COMMENT: 52e0036cabf3303e 3 wMif7ZQIqXao1UAn/FlMZKbQPuU (comment: cdc18-150-577(T374A) when expressed on multi copy plasmid does not rescue cdc18-K46) ------- COMMENT: 52e0036cabf3303e 4 7VvMO7mOti/Xcl4W31Lum5OqARs (comment: cdc1-577 (NTP) when expressed on multi copy plasmid does not rescue cdc18-K46) ------- COMMENT: 52e0036cabf3303e 5 2fK8L1l9WULH2RE8jbbgnzDbDmc Fig 2A (comment: cdc18 expressed from pREP3X and assayed after 20 hours after removal of thiamine at 32°C) ------- COMMENT: 52e0036cabf3303e 7 2fK8L1l9WULH2RE8jbbgnzDbDmc Fig 2A (comment: cdc18 expressed from pREP3X and assayed after 20 hours after removal of thiamine at 32°C) ------- COMMENT: 52e0036cabf3303e 8 2fK8L1l9WULH2RE8jbbgnzDbDmc Fig 2A (comment: cdc18 expressed from pREP3X and assayed after 20 hours after removal of thiamine at 32°C) ------- COMMENT: 52e0036cabf3303e 9 2fK8L1l9WULH2RE8jbbgnzDbDmc Fig 2A (comment: cdc18 expressed from pREP3X and assayed after 20 hours after removal of thiamine at 32°C) ------- COMMENT: 52e0036cabf3303e 10 fNkOEkE7mKxUmHIqe//xTBaMVAs Fig 2B (comment: cdc18 expressed from pREP3X and assayed after 20 hours after removal of thiamine at 32°C) ------- COMMENT: 52e0036cabf3303e 11 mzx0IvYWmPhEG/jP2u87qn/0eJ8 Fig 2B (comment: dc18 expressed from pREP3X and assayed after 20 hours after removal of thiamine at 32°C) ------- COMMENT: 52e0036cabf3303e 12 l2ycns2SZZIRF7w7jPz/9XaFPq0 Fig 3 (comment: the kinase assay substrate used is Histone H1) ------- COMMENT: 52e0036cabf3303e 13 l2ycns2SZZIRF7w7jPz/9XaFPq0 Fig 3 (comment: the kinase assay substrate used is Histone H1) ------- COMMENT: 52e0036cabf3303e 14 bf4UPxXiOUNnCry+6KIiZjrCeBw data not shown (comment: the kinase assay substrate used is Histone H1) ------- COMMENT: 52e0036cabf3303e 15 Hk3meG1Aj9I8IrUZKImHhSHhtGw Fig 3 (comment: cdc18 expressed from pREP3X and assayed for 20 hours after removal of thiamine at 32°C) ------- COMMENT: 52e0036cabf3303e 16 Hk3meG1Aj9I8IrUZKImHhSHhtGw Fig 3 (comment: cdc18 expressed from pREP3X and assayed for 20 hours after removal of thiamine at 32°C) ------- COMMENT: 52e0036cabf3303e 17 Hk3meG1Aj9I8IrUZKImHhSHhtGw Fig 3 (comment: cdc18 expressed from pREP3X and assayed for 20 hours after removal of thiamine at 32°C) ------- COMMENT: 52e0036cabf3303e 18 K+aRSLh3h/2ipaqv/+vN60SsX2Y Fig 3 (comment: not strictly a co-immunoprecitation experiment as they used suc1 beads to pull down cdc2 then a western blot) ------- COMMENT: 52e0036cabf3303e 19 K+aRSLh3h/2ipaqv/+vN60SsX2Y Fig 3 (comment: not strictly a co-immunoprecitation experiment as they used suc1 beads to pull down cdc2 then a western blot) ------- COMMENT: 52e0036cabf3303e 20 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 21 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 22 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 23 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 24 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 25 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 26 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 27 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 28 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 29 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 30 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 31 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 32 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 33 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 34 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 36 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 37 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 38 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 39 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 40 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 41 2fK8L1l9WULH2RE8jbbgnzDbDmc Fig 2A (comment: cdc18 expressed from pREP3X and assayed after 20 hours after removal of thiamine at 32°C) ------- COMMENT: 52e0036cabf3303e 42 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 43 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 44 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 45 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 46 a8x+NJBES9dEw1SBWf1GPdEuKgc Fig 4 (comment: cdc18 expressed from nmt1 on multi copy plasmid ------- COMMENT: 52e0036cabf3303e 47 rDI/Ua8aG77oLBNwq/2tz4fMJCw Data not shown (comment: Cdc18 expressed from nmt1 promoter on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 48 rDI/Ua8aG77oLBNwq/2tz4fMJCw Data not shown (comment: Cdc18 expressed from nmt1 promoter on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 49 jadrp78nxUwWJMoNRzJl4OjJ19c Fig 5 (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid. Cells grown in absence of thiamine for 20 hours then shifted to 25°C or 36°C and followed for 3 generations) ------- COMMENT: 52e0036cabf3303e 50 jadrp78nxUwWJMoNRzJl4OjJ19c Fig 5 (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid. Cells grown in absence of thiamine for 20 hours then shifted to 25°C or 36°C and followed for 3 generations) ------- COMMENT: 52e0036cabf3303e 51 jadrp78nxUwWJMoNRzJl4OjJ19c Fig 5 (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid. Cells grown in absence of thiamine for 20 hours then shifted to 25°C or 36°C and followed for 3 generations) ------- COMMENT: 52e0036cabf3303e 52 jadrp78nxUwWJMoNRzJl4OjJ19c Fig 5 (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid. Cells grown in absence of thiamine for 20 hours then shifted to 25°C or 36°C and followed for 3 generations) ------- COMMENT: 52e0036cabf3303e 53 jadrp78nxUwWJMoNRzJl4OjJ19c Fig 5 (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid. Cells grown in absence of thiamine for 20 hours then shifted to 25°C or 36°C and followed for 3 generations) ------- COMMENT: 52e0036cabf3303e 55 jadrp78nxUwWJMoNRzJl4OjJ19c Fig 5 (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid. Cells grown in absence of thiamine for 20 hours then shifted to 25°C or 36°C and followed for 3 generations) ------- COMMENT: 52e0036cabf3303e 56 jadrp78nxUwWJMoNRzJl4OjJ19c Fig 5 (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid. Cells grown in absence of thiamine for 20 hours then shifted to 25°C or 36°C and followed for 3 generations) ------- COMMENT: 52e0036cabf3303e 57 jadrp78nxUwWJMoNRzJl4OjJ19c Fig 5 (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid. Cells grown in absence of thiamine for 20 hours then shifted to 25°C or 36°C and followed for 3 generations) ------- COMMENT: 52e0036cabf3303e 58 jadrp78nxUwWJMoNRzJl4OjJ19c Fig 5 (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid. Cells grown in absence of thiamine for 20 hours then shifted to 25°C or 36°C and followed for 3 generations) ------- COMMENT: 52e0036cabf3303e 59 jadrp78nxUwWJMoNRzJl4OjJ19c Fig 5 (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid. Cells grown in absence of thiamine for 20 hours then shifted to 25°C or 36°C and followed for 3 generations) ------- COMMENT: 52e0036cabf3303e 60 tKziHxPB0V+hJWatArOG0VYHGUw Fig 6 (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid. cells examined after 20 h after thiamine removal.) ------- COMMENT: 52e0036cabf3303e 61 tKziHxPB0V+hJWatArOG0VYHGUw Fig 6 (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid. cells examined after 20 h after thiamine removal) ------- COMMENT: 52e0036cabf3303e 62 tKziHxPB0V+hJWatArOG0VYHGUw Fig 6 (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid. cells examined after 20 h after thiamine removal) ------- COMMENT: 52e0036cabf3303e 63 tKziHxPB0V+hJWatArOG0VYHGUw Fig 6 (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid. cells examined after 20 h after thiamine removal) ------- COMMENT: 52e0036cabf3303e 64 tKziHxPB0V+hJWatArOG0VYHGUw Fig 6 (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid. cells examined after 20 h after thiamine removal) ------- COMMENT: 52e0036cabf3303e 65 0Sr3q0L0hWciailjtOlKXQeXPkg Fig 6. (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 66 0Sr3q0L0hWciailjtOlKXQeXPkg Fig 6. (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 67 0Sr3q0L0hWciailjtOlKXQeXPkg Fig 6. (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 68 0Sr3q0L0hWciailjtOlKXQeXPkg Fig 6. (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 69 0Sr3q0L0hWciailjtOlKXQeXPkg Fig 6. (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 70 0Sr3q0L0hWciailjtOlKXQeXPkg Fig 6. (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 71 0Sr3q0L0hWciailjtOlKXQeXPkg Fig 6. (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 72 42pWMwp/oOedI9wZNbwy2/emdVY Fig 6 (comment: and cell phenotype data not shown. cdc18 expressed from nmt1 promoter on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 73 0Sr3q0L0hWciailjtOlKXQeXPkg Fig 6. (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid) ------- COMMENT: 52e0036cabf3303e 74 0Sr3q0L0hWciailjtOlKXQeXPkg Fig 6. (comment: cdc18 expressed from nmt1 promoter on multi copy plasmid) ------- COMMENT: 5309bbb3babb1fef 1 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 5309bbb3babb1fef 2 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 5309bbb3babb1fef 3 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 5309bbb3babb1fef 4 7aWrVY8UjRAefTjtF1Bv4S+P4lQ Microscopy revealed occasional cells with an aberrant septum or with multiple nuclei Fig. 2A ------- COMMENT: 5309bbb3babb1fef 5 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 5309bbb3babb1fef 6 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 5309bbb3babb1fef 7 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 5309bbb3babb1fef 8 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 5309bbb3babb1fef 9 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 5309bbb3babb1fef 10 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 5309bbb3babb1fef 11 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 5309bbb3babb1fef 12 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 5309bbb3babb1fef 13 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 5309bbb3babb1fef 14 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 5339c3839d6a7634 4 Zno40JIIfMde9u5cNVEbdVzh+iw Fig. 1B,C ------- COMMENT: 5339c3839d6a7634 6 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 5339c3839d6a7634 8 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 5339c3839d6a7634 10 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 5339c3839d6a7634 12 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 5339c3839d6a7634 14 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 5339c3839d6a7634 16 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 5339c3839d6a7634 18 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 5339c3839d6a7634 20 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 5339c3839d6a7634 22 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 5339c3839d6a7634 23 tgJlKqbf+dsqrVa0sQtk/ABI8A8 Taken together, these results show that H3K4ac exists in S. pombe, and that Mst1 and the sirtuin Sir2 are the major HAT and HDAC, respectively, for H3K4. Fig. 2 ------- COMMENT: 5339c3839d6a7634 25 L8GOG7c/Gwr4VAdAVshmEWTFyr8 (comment: IMP evidence for sir2 being the major HDAC, IDA for mst1 being HAT) ------- COMMENT: 5339c3839d6a7634 41 IgMaceIZ231d1S3E/q+kBcsqFpo Fig. 4H ------- COMMENT: 5339c3839d6a7634 42 IgMaceIZ231d1S3E/q+kBcsqFpo Fig. 4H ------- COMMENT: 5339c3839d6a7634 43 IgMaceIZ231d1S3E/q+kBcsqFpo Fig. 4H ------- COMMENT: 5339c3839d6a7634 45 4LswAxnQboEmiHSeKVl1mpliZ2g Fig. 4I ------- COMMENT: 5339c3839d6a7634 46 4LswAxnQboEmiHSeKVl1mpliZ2g Fig. 4I ------- COMMENT: 5339c3839d6a7634 47 4LswAxnQboEmiHSeKVl1mpliZ2g Fig. 4I ------- COMMENT: 5339c3839d6a7634 49 4LswAxnQboEmiHSeKVl1mpliZ2g Fig. 4I ------- COMMENT: 5339c3839d6a7634 50 4LswAxnQboEmiHSeKVl1mpliZ2g Fig. 4I ------- COMMENT: 5339c3839d6a7634 51 4LswAxnQboEmiHSeKVl1mpliZ2g Fig. 4I ------- COMMENT: 5339c3839d6a7634 53 Zno40JIIfMde9u5cNVEbdVzh+iw Fig. 1B and C ------- COMMENT: 5339c3839d6a7634 54 Zno40JIIfMde9u5cNVEbdVzh+iw Fig. 1B and C ------- COMMENT: 5339c3839d6a7634 55 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 5339c3839d6a7634 56 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 5339c3839d6a7634 57 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 5339c3839d6a7634 58 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 5339c3839d6a7634 59 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 5339c3839d6a7634 60 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 5339c3839d6a7634 61 eXAsJ0A+UXyv1NxjDde9uhqujL4 Taken together, these results show that H3K4ac exists in S. pombe, and that Mst1 and the sirtuin Sir2 are the major HAT and HDAC, respectively, for H3K4. ------- COMMENT: 5339c3839d6a7634 62 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 5339c3839d6a7634 63 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 5339c3839d6a7634 64 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 5339c3839d6a7634 65 vkT8uMpiBxgGlmYXEA8V3+LXigM Fig. 4E ------- COMMENT: 5339c3839d6a7634 66 9Xb+uz4ftlqagKGp7TY1gJc7WdA Fig. 4F ------- COMMENT: 5339c3839d6a7634 67 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 5342aa989c2f1d8a 2 ACPiaqBRmH7xhgQI0Tv2cF5mDU8 Fig2 ------- COMMENT: 5342aa989c2f1d8a 5 4Aq34geVA2LBNhG6GAIbP7Bn4yo (comment: CHECK fix catalytic activity) ------- COMMENT: 5342aa989c2f1d8a 7 4Aq34geVA2LBNhG6GAIbP7Bn4yo (comment: CHECK fix catalytic activity) ------- COMMENT: 536dc2e074eee139 47 l165mgamoT9Y90wthcjZQM/bn5E table 1 ------- COMMENT: 536dc2e074eee139 48 l165mgamoT9Y90wthcjZQM/bn5E table 1 ------- COMMENT: 536dc2e074eee139 49 l165mgamoT9Y90wthcjZQM/bn5E table 1 ------- COMMENT: 536dc2e074eee139 50 l165mgamoT9Y90wthcjZQM/bn5E table 1 ------- COMMENT: 536dc2e074eee139 51 9BSDRu77gCcpiKi803iu7V3zM+k table 2 ------- COMMENT: 536dc2e074eee139 52 9BSDRu77gCcpiKi803iu7V3zM+k table 2 ------- COMMENT: 536dc2e074eee139 53 /BZjPAvUyZcRggNcLIb+lQAvvFI table2 ------- COMMENT: 536dc2e074eee139 54 /BZjPAvUyZcRggNcLIb+lQAvvFI table2 ------- COMMENT: 536dc2e074eee139 55 /BZjPAvUyZcRggNcLIb+lQAvvFI table2 ------- COMMENT: 536dc2e074eee139 56 /BZjPAvUyZcRggNcLIb+lQAvvFI table2 ------- COMMENT: 536dc2e074eee139 57 /BZjPAvUyZcRggNcLIb+lQAvvFI table2 ------- COMMENT: 536dc2e074eee139 58 /BZjPAvUyZcRggNcLIb+lQAvvFI table2 ------- COMMENT: 536dc2e074eee139 59 /BZjPAvUyZcRggNcLIb+lQAvvFI table2 ------- COMMENT: 536dc2e074eee139 60 l165mgamoT9Y90wthcjZQM/bn5E table 1 ------- COMMENT: 536dc2e074eee139 61 l165mgamoT9Y90wthcjZQM/bn5E table 1 ------- COMMENT: 536dc2e074eee139 62 9BSDRu77gCcpiKi803iu7V3zM+k table 2 ------- COMMENT: 536dc2e074eee139 64 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 536dc2e074eee139 65 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 536dc2e074eee139 66 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 536dc2e074eee139 67 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 536dc2e074eee139 68 xSgq/1PyvS+43QiBpmVp7HA6Uvw fig 2a ------- COMMENT: 536dc2e074eee139 69 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 536dc2e074eee139 70 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 536dc2e074eee139 71 xSgq/1PyvS+43QiBpmVp7HA6Uvw fig 2a ------- COMMENT: 536dc2e074eee139 72 xSgq/1PyvS+43QiBpmVp7HA6Uvw fig 2a ------- COMMENT: 536dc2e074eee139 73 hQqbdkFhynnTdHzII0cazcBO4VQ table 3 ------- COMMENT: 536dc2e074eee139 74 hQqbdkFhynnTdHzII0cazcBO4VQ table 3 ------- COMMENT: 536dc2e074eee139 75 hQqbdkFhynnTdHzII0cazcBO4VQ table 3 ------- COMMENT: 536dc2e074eee139 80 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: 536dc2e074eee139 81 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: 536dc2e074eee139 84 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: 536dc2e074eee139 85 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: 53712c93077e090e 1 rZlNvEASHIfA5PeMzHbLHJWfPo0 ------- COMMENT: 53712c93077e090e 28 CIsoywDX5OzcU4oDPymzeCsCr7o (comment: using chromosome III) ------- COMMENT: 53af9f6726121d19 64 +XJeAPbZ6pyukzLF5erSXlQmTNk (comment: Observed in cells undergoing vegetative growth) ------- COMMENT: 53af9f6726121d19 65 +XJeAPbZ6pyukzLF5erSXlQmTNk (comment: Observed in cells undergoing vegetative growth) ------- COMMENT: 53af9f6726121d19 66 +XJeAPbZ6pyukzLF5erSXlQmTNk (comment: Observed in cells undergoing vegetative growth) ------- COMMENT: 53af9f6726121d19 67 +XJeAPbZ6pyukzLF5erSXlQmTNk (comment: Observed in cells undergoing vegetative growth) ------- COMMENT: 53af9f6726121d19 68 +XJeAPbZ6pyukzLF5erSXlQmTNk (comment: Observed in cells undergoing vegetative growth) ------- COMMENT: 53af9f6726121d19 69 +XJeAPbZ6pyukzLF5erSXlQmTNk (comment: Observed in cells undergoing vegetative growth) ------- COMMENT: 53af9f6726121d19 77 BbMa+F9aLWd4GpCf0V5HrFHD0zA (comment: CHECK mei4 ssm4 crs1 rec8 spo5) ------- COMMENT: 53af9f6726121d19 79 BbMa+F9aLWd4GpCf0V5HrFHD0zA (comment: CHECK mei4 ssm4 crs1 rec8 spo5) ------- COMMENT: 53af9f6726121d19 113 692FcKfF464z9FMoaFbKs3Q341Q (comment: CHECK mei4 ssm4 crs1 rec8 spo5) ------- COMMENT: 53af9f6726121d19 194 hfYxCEskwrmCXoiD9bRwpLRcaDM ------- COMMENT: 53b4f5e5547d3733 3 e6SyT0/p5Y83SnYZSQd41cVuY0M (comment: binding site L405 ndc80 loop ------- COMMENT: 53b4f5e5547d3733 26 cqbqES36zAlnCEknB8GBixDaDec (comment: affecting dis1 ------- COMMENT: 53e0b793e37b4408 2 fA2IkikjKiznolwhJqA+gKuAJWs (comment: Pcn1 can interact with Pxd1 N-terminal fragment (1-73) in vitro ------- COMMENT: 53e0b793e37b4408 5 kP1V6VLMbwQS1rIHjcWVYU/tGKA The deletion of pxd1 suppressed the CPT sensitivity of the cdt2Δ spd1Δ mutant ------- COMMENT: 53e0b793e37b4408 6 Vd3KmKkY23DfajF8GK1Nm+O0MJc ddb1Δ was synthetic lethal with dna2-TAP and this synthetic lethality was suppressed by the deletion of pxd1 ------- COMMENT: 53e0b793e37b4408 14 6Jhsiw0O8O1ey5p93gLjHwMlO2E Pxd1 N-terminal fragment (1-73) can interact with Pcn1 in vitro ------- COMMENT: 53e0b793e37b4408 15 Me6xK0KLywAmmTyq3Aojz7cUCoE Pxd1-PIP5A is synthetic lethal with dna2-c2 or dna2-TAP ------- COMMENT: 53e0b793e37b4408 16 K2AE+oQjXJIZraEbMQta3goeEHM cdt2Δ was synthetic lethal with dna2-TAP and this synthetic lethality was suppressed by the deletion of pxd1 ------- COMMENT: 53e0b793e37b4408 23 OAjyTWlMra/fNB5BXvV2Nyv9gfg dna2-c2 or dna2-TAP is synthetic lethal with Pxd1-PIP5A ------- COMMENT: 53e0b793e37b4408 24 TM6R1L8fsxCcN7c666pVMLOJQHg dna2-TAP was synthetic lethal with cdt2Δ and this synthetic lethality was suppressed by the deletion of pxd1 ------- COMMENT: 53e0b793e37b4408 25 Sle13lWFZ6g8tOr8Zm7X9cq/bDs dna2-TAP was synthetic lethal with ddb1Δ and this synthetic lethality was suppressed by the deletion of pxd1 ------- COMMENT: 53e0b793e37b4408 68 KmGnnjLlzZDhS4y2+okXIOpsiYY (comment: regulates pathway choice) ------- COMMENT: 53e97b95347633ef 1 1ogCnE5Xw0RLnWYCTwr0MTcFWeU ChIP-Seq data showed binding to both heterochromatin and euchromatin. ------- COMMENT: 53e97b95347633ef 2 1ogCnE5Xw0RLnWYCTwr0MTcFWeU ChIP-Seq data showed binding to both heterochromatin and euchromatin. ------- COMMENT: 53e97b95347633ef 22 1ogCnE5Xw0RLnWYCTwr0MTcFWeU ChIP-Seq data showed binding to both heterochromatin and euchromatin. ------- COMMENT: 53e97b95347633ef 29 xpi1U4S5bq5cxSWXUWAyO24tur8 Mutating the Php5 binding site CCAAT box at cenH causes a defect in heterochromatin formation at the mating type locus. ------- COMMENT: 53e97b95347633ef 30 SG1/eOupC1an8a5Wt4zqy4e0OuA Mutations in the php5 gene or its binding site, the CCAAT box located at cenH when combine with Swi6, result in a significant decrease in siRNA production at the mating type locus. ------- COMMENT: 53e97b95347633ef 31 znwB0wk1gvrlm9ymx6+jL4+bc/Y RNA seq and RT-PCR data showed Php5 required for cenH expression. ------- COMMENT: 53e97b95347633ef 32 1ogCnE5Xw0RLnWYCTwr0MTcFWeU ChIP-Seq data showed binding to both heterochromatin and euchromatin. ------- COMMENT: 53e97b95347633ef 35 YA8tvJfNZGI6rxi+38tQOpbgBaQ Mutating the Php3 binding site CCAAT box at cenH causes a defect in heterochromatin formation at the mating type locus. ------- COMMENT: 53e97b95347633ef 36 2eyg6h+rXLbaBp+pEcAJzlQ/hfk Mutations in the Php3 gene or its binding site, the CCAAT box located at cenH, result in a significant decrease in siRNA production at the mating type locus. ------- COMMENT: 53e97b95347633ef 37 nUVyyHX64dbaeo3P0tO2PO6DdoM RNA seq and RT-PCR data showed Php3 required for cenH expression. ------- COMMENT: 53e97b95347633ef 38 0RBrBhbw0sFsj86kpGmdkfC/+BM ChIP-Seq data showed binding to euchromatin. ------- COMMENT: 53e97b95347633ef 39 KJSjGtTJPn7WBbgmQe32tuJ22L4 ChIP-Seq data showed binding to lncRNA ------- COMMENT: 53e97b95347633ef 40 1ogCnE5Xw0RLnWYCTwr0MTcFWeU ChIP-Seq data showed binding to both heterochromatin and euchromatin. ------- COMMENT: 53e97b95347633ef 41 1ogCnE5Xw0RLnWYCTwr0MTcFWeU ChIP-Seq data showed binding to both heterochromatin and euchromatin. ------- COMMENT: 53e97b95347633ef 42 0RBrBhbw0sFsj86kpGmdkfC/+BM ChIP-Seq data showed binding to euchromatin. ------- COMMENT: 53e97b95347633ef 43 0RBrBhbw0sFsj86kpGmdkfC/+BM ChIP-Seq data showed binding to euchromatin. ------- COMMENT: 53e97b95347633ef 44 0RBrBhbw0sFsj86kpGmdkfC/+BM ChIP-Seq data showed binding to euchromatin. ------- COMMENT: 53e97b95347633ef 45 0RBrBhbw0sFsj86kpGmdkfC/+BM ChIP-Seq data showed binding to euchromatin. ------- COMMENT: 53e97b95347633ef 46 0RBrBhbw0sFsj86kpGmdkfC/+BM ChIP-Seq data showed binding to euchromatin. ------- COMMENT: 53e97b95347633ef 47 0RBrBhbw0sFsj86kpGmdkfC/+BM ChIP-Seq data showed binding to euchromatin. ------- COMMENT: 53e97b95347633ef 48 0RBrBhbw0sFsj86kpGmdkfC/+BM ChIP-Seq data showed binding to euchromatin. ------- COMMENT: 53e97b95347633ef 49 0RBrBhbw0sFsj86kpGmdkfC/+BM ChIP-Seq data showed binding to euchromatin. ------- COMMENT: 53e97b95347633ef 50 A7tVP6bjUrHdI4x2NcA0PH8fwNM Small RNA sequencing data indicated that Swi6 is necessary for siRNA production, irrespective of PhpC and Moc3. ------- COMMENT: 53fad494a10fe0a6 1 85pP4srojScRRqGuS7KzqG/aul8 How- ever, rec11 cells often contain three or four cut3-GFP dots (Fig. 1A), representing dissoci- ation in these regions, as observed in rec8 cells. ------- COMMENT: 53fad494a10fe0a6 2 IMHms2FWtBIsCBN6UmElE7jDX6Q figure 1B ------- COMMENT: 53fad494a10fe0a6 3 IMHms2FWtBIsCBN6UmElE7jDX6Q figure 1B ------- COMMENT: 53fad494a10fe0a6 4 IMHms2FWtBIsCBN6UmElE7jDX6Q figure 1B ------- COMMENT: 53fad494a10fe0a6 5 mAI+E5h9exWudmbsEkJ7nxhwqiE Experiments in which cen3 were marked with GFP on both homologs revealed that 20% of rec11 cells exhibit homolog nondis- junction, in which both homolog pairs move to the same pole at meiosis I (Fig. 1C). ------- COMMENT: 53fad494a10fe0a6 6 4oEdkQRPTneQoDehRuMRnKDtSqQ Nonrandom segregation of homologs in rec11  cells (different from rec12  cells) can be explained by residual levels of recombination (15) and, presumably, residual cohesion as well. rec11  cells undergo faithful disjunction during meiosis II (fig. S2C), indicating that centromeric cohesion persists through meiosis I. ------- COMMENT: 53fad494a10fe0a6 7 lKMZ5ETjTew2tSIMaiaQQ5pbur0 Mitotic cells carrying a temperature-sensitive allele of psc3 (psc3-2T) (18) displayed extensive separation of cut3-GFP dots (fig. S2B), ------- COMMENT: 53fad494a10fe0a6 8 gsZlvu8ipUIdVThIu5FrhVmqagU whereas arm cohesion of psc3-2T cells is completely intact during meiotic prophase (fig. S2C). ------- COMMENT: 53fad494a10fe0a6 9 2PZwzM+1cl/iQ+H3+WuGwb2dF2c In contrast to the dramatic reduction of meiotic recombination in rec11  cells, wild-type levels of recombination occur in psc3-2T cells (fig. S2D). ------- COMMENT: 53fad494a10fe0a6 10 21IjeHtmWFjCWsY4fiLP1Ur3CNQ Moreover, the overexpression of psc3  leads to partial recovery of the arm cohesion defect of rec11 , but the recombination defect remains unimproved (fig. S3). ------- COMMENT: 53fad494a10fe0a6 12 21IjeHtmWFjCWsY4fiLP1Ur3CNQ Moreover, the overexpression of psc3  leads to partial recovery of the arm cohesion defect of rec11 , but the recombination defect remains unimproved (fig. S3). ------- COMMENT: 53fad494a10fe0a6 13 GbSGQaIfgcNYC73qEl5XzauLxzM Indeed, psc3-2T cells show defects in sister chromatid segregation during meiosis (fig. S2E). To ------- COMMENT: 53fad494a10fe0a6 14 QGru4aPQsYq9NDUHSU2P0W2opvA The control rec8  overexpressing (o.p.) psc3  rec11  cells undergo proper meiotic chromosome segregation, both reductional (meiosis I) and equational (meiosis II). ------- COMMENT: 53fad494a10fe0a6 15 QGru4aPQsYq9NDUHSU2P0W2opvA The control rec8  overexpressing (o.p.) psc3  rec11  cells undergo proper meiotic chromosome segregation, both reductional (meiosis I) and equational (meiosis II). ------- COMMENT: 53fad494a10fe0a6 16 8Cmld57rU3PdANqOZn4SSPwDj58 expression of the Rec8-Rec11 pair sustains viability of rad21  psc3  cells (Fig. 1D), thus allowing meiotic induction in the complete absence of Psc3). However, rec8 o.p. psc3  rec11 o.p. cellsshow defective sister chromatid movement atboth meiotic divisions (Fig. 1D). ------- COMMENT: 53fad494a10fe0a6 17 DwE/SEsMNoZslxIJiLB4XTOQgWI expression of the Rec8-Rec11 pair sustains viability of rad21  psc3  cells (Fig. 1D), thus allowing meiotic induction in the complete absence of Psc3However, rec8 o.p. psc3  rec11 o.p. cellsshow defective sister chromatid movement atboth meiotic divisions (Fig. 1D). ------- COMMENT: 53fad494a10fe0a6 18 zPKH1MPrLBOXj+v1+Lo+3CtzE6k Thus, Psc3 plays a crucial role in kinetochore regulation at both meiotic divisions, and this function of Psc3 cannot be replaced by overexpression of Rec11. ------- COMMENT: 53fad494a10fe0a6 19 zPKH1MPrLBOXj+v1+Lo+3CtzE6k Thus, Psc3 plays a crucial role in kinetochore regulation at both meiotic divisions, and this function of Psc3cannot be replaced by overexpression of Rec11. ------- COMMENT: 53fad494a10fe0a6 20 jlA2Ak+fDcpBBmxeuzi2cXhe5tc In contrast, the Rec8-Rec11 pair sustains mitotic growth better than Rec8-Psc3 (Fig. 1D; fig. S4A), underscoring the meiosis specificity of kinetochore regulation by Rec8-Psc3. ------- COMMENT: 53fad494a10fe0a6 22 4Wiw7OeFb2ydopC26dYd+UVMLUE During meiotic prophase, Rec8 first appears at thecentromeres and later distributes throughout thechromosome (Fig. 2A) The data reveal that Rec8 associates more with centromere regions than with chromosome arms (20), ------- COMMENT: 53fad494a10fe0a6 23 TmVU3BDMDUOVUsnjc2OJxULTdAI In contrast, Psc3 associates exclusively with the centromere, showing a centromere-enrichment ratio 4 times higher than Rec8 and 20 times higher than Rec11 (Fig. 2B). ------- COMMENT: 53fad494a10fe0a6 24 vIOlGKheQpqQvd26xWi3a1Ar6tE At anaphase of meiosis I, Rec11 signals become faint in the nucleus, but Psc3 persists, together with Rec8, at the clustered centromeres (Fig. 2C).The centromeric Rec8-Psc3 dots disappear at meiosis II. ------- COMMENT: 53fad494a10fe0a6 25 j/VlvMbmHSzihT7XflOfTXTvIqo At anaphase of meiosis I, Rec11 signals become faint in the nucleus, but Psc3 persists, together with Rec8, at the clustered centromeres (Fig. 2C). The centromeric Rec8-Psc3 dots disappear at meiosis II. ------- COMMENT: 53fad494a10fe0a6 26 5GwE8FYILMLienX6WBznoDqSDww At anaphase of meiosis I, Rec11 signals become faint in the nucleus, ------- COMMENT: 53fad494a10fe0a6 27 qxlTIQArLTS8LbHrcaKEj9BgoH4 Furthermore, immunoprecipitation experiments demonstrated that Rec8 interacts with Psc3, Rec11, and Psm3/Smc3 in vivo (fig. S5). At anaphase of meiosis I, ------- COMMENT: 53fad494a10fe0a6 28 qxlTIQArLTS8LbHrcaKEj9BgoH4 Furthermore, immunoprecipitation experiments demonstrated that Rec8 interacts with Psc3, Rec11, and Psm3/Smc3 in vivo (fig. S5). At anaphase of meiosis I, ------- COMMENT: 53fad494a10fe0a6 29 qxlTIQArLTS8LbHrcaKEj9BgoH4 Furthermore, immunoprecipitation experiments demonstrated that Rec8 interacts with Psc3, Rec11, and Psm3/Smc3 in vivo (fig. S5). At anaphase of meiosis I, ------- COMMENT: 53fad494a10fe0a6 30 DR+TE9yMDhChp4Ncn8Z6JzDb5PY (comment: CHECK MEIOTIC!********************) The level of Rec8 association with pericentromeric regions is markedly reduced in these mutants, whereas Rec8 is still enriched at the central core (Fig. 3A). ------- COMMENT: 53fad494a10fe0a6 31 Soli8Ca4u4Ay+k5Kgvo37pXCnVw (comment: CHECK MEIOTIC!***********************) The level of Rec8 association with pericentromeric regions is markedly reduced in these mutants, whereas Rec8 is still enriched at the central core (Fig. 3A). ------- COMMENT: 53fad494a10fe0a6 32 Z+dW9Zg84a+9v0G0MEmh18f/ygs (comment: CHECK MEIOTIC!MEIOTIC!***********************) The level of Rec8 association with pericentromeric regions is markedly reduced in these mutants, whereas Rec8 is still enriched at the central core (Fig. 3A). ------- COMMENT: 53fad494a10fe0a6 33 Z+dW9Zg84a+9v0G0MEmh18f/ygs (comment: CHECK MEIOTIC!MEIOTIC!***********************) The level of Rec8 association with pericentromeric regions is markedly reduced in these mutants, whereas Rec8 is still enriched at the central core (Fig. 3A). ------- COMMENT: 53fad494a10fe0a6 34 ZOex7mzbbBSWwwAE3hB97sx3lmU (comment: CHECK MEIOTIC!MEIOTIC.*************) whereas Rec8 is still enriched at the central core (Fig. 3A). ------- COMMENT: 53fad494a10fe0a6 35 ZOex7mzbbBSWwwAE3hB97sx3lmU (comment: CHECK MEIOTIC!MEIOTIC.*************) whereas Rec8 is still enriched at the central core (Fig. 3A). ------- COMMENT: 53fad494a10fe0a6 36 8E7Act1uFs0q6LF9vL7605FExRc In swi6  and clr4  cells marked on one pair of sister chromatids with cen1-GFP, sister chromatid pairs move together to the same nucleus during meiosis I, indicating that monopolar attachment is intact in these mutants (Fig. 3B). ------- COMMENT: 53fad494a10fe0a6 37 D+mpkYws47ItSALr9oExoCyLqyA Moreover, homologous chromosomes undergo faithful disjunction at meiosis I in these mutants (Fig. 3C) (17). ------- COMMENT: 53fad494a10fe0a6 38 8E7Act1uFs0q6LF9vL7605FExRc In swi6  and clr4  cells marked on one pair of sister chromatids with cen1-GFP, sister chromatid pairs move together to the same nucleus during meiosis I, indicating that monopolar attachment is intact in these mutants (Fig. 3B). ------- COMMENT: 53fad494a10fe0a6 39 D+mpkYws47ItSALr9oExoCyLqyA Moreover, homologous chromosomes undergo faithful disjunction at meiosis I in these mutants (Fig. 3C) (17). ------- COMMENT: 53fad494a10fe0a6 40 r+CDQ9mVw7M7L36aD7PJmjw9Kdc At meiosis II, however, sisters fail to segregate properly, undergoing nondisjunction in 20 to 40% of cells (Fig. 3B). ------- COMMENT: 53fad494a10fe0a6 41 pEfWh4i8A5qE1kEPDgu+tkczk+4 At meiosis II, however, sisters fail to segregate properly, undergoing nondisjunction in 20 to 40% of cells (Fig. 3B). The defect in meiosis II is more penetrating in clr4  cells than in swi6  cells, with a pattern approximating random segregation. ------- COMMENT: 53fad494a10fe0a6 42 a5u6gZINygrCli6HjrNs+NHsLSk (comment: CHECK ******premature sister kinetochore separation in meiosis I********) Reinforcing the foregoing results, clr4  cells frequently display precocious separation of cen2-GFP signals (Fig. 3C) and a decreased level of Rec8 at the centromeres if arrested after meiosis I (Fig. 3D). ------- COMMENT: 54907a8f92a3eb84 56 TVG6wzJamNQH923amxmJaVAnq4c inferred from abolished interaction between Pof8 and Lsm subunits ------- COMMENT: 54907a8f92a3eb84 57 TVG6wzJamNQH923amxmJaVAnq4c inferred from abolished interaction between Pof8 and Lsm subunits ------- COMMENT: 54907a8f92a3eb84 58 haVP/r0vnj09z/TtCSWyju+a5NQ inferred from decreased interaction between Pof8 and Lsm subunits ------- COMMENT: 54af20fd9bd9f581 74 MYkznQmI86N0ia+DsEE/b0ut4xk From review: Rab small GTPase emerges as a regulator of TOR complex 2 "Consistently, we successfully collected genetic and biochemical data to support the notion that Sat1 and Sat4 form a GEF complex for Ryh1 GTPase in S. pombe. (7). ------- COMMENT: 54af20fd9bd9f581 75 MYkznQmI86N0ia+DsEE/b0ut4xk From review: Rab small GTPase emerges as a regulator of TOR complex 2 "Consistently, we successfully collected genetic and biochemical data to support the notion that Sat1 and Sat4 form a GEF complex for Ryh1 GTPase in S. pombe. (7). ------- COMMENT: 54af20fd9bd9f581 76 EXNwYU2zlhMacfm8QomkzpJ+2z4 (comment: CHECK this is a bit of a fudge. It should probably be molecular signal transducer but that does not exist and I am prevented from using high level terms) vw: From review:Rab small GTPase emerges as a regulator of TOR complex 2"Consistently, we successfully collected genetic and biochemical data to supportthe notion that Sat1 and Sat4 form a GEF complex for Ryh1 GTPase in S. pombe. (7).I agree the data supports this model. Moreover, the Ryh1 I44E mutant fails to promote TORC2 signaling, implying that GTP-dependent interaction of Ryh1 with TORC2 via the effector domain drives TORC2-Gad8 signaling. ------- COMMENT: 54af20fd9bd9f581 77 LPyQRdBiotJ+6mCjjzO3XZKBTvY (comment: vw: bit61 is importabt for torc2 regulation by ryh1) ------- COMMENT: 54db84e4ca91c654 178 +fqN4QeaacT7CrV6d6d1LWCXevM (comment: CHECK cisplatin sensitivity) ------- COMMENT: 54db84e4ca91c654 179 2jf/rqcXwNaQO/21YNdLkuAz9mA (comment: CHECK 4-nitroquinoline N-oxide sensitivity) ------- COMMENT: 54db84e4ca91c654 180 +fqN4QeaacT7CrV6d6d1LWCXevM (comment: CHECK cisplatin sensitivity) ------- COMMENT: 54db84e4ca91c654 181 ucVIx+e4w1j8wDaOgLtYTU5jGME (comment: CHECK chromium sensitivity) ------- COMMENT: 54db84e4ca91c654 182 aJjQfpx870H2IpSBeUOAdDfboPo (comment: CHECK camptothecin sensitivity) ------- COMMENT: 54db84e4ca91c654 183 ucVIx+e4w1j8wDaOgLtYTU5jGME (comment: CHECK chromium sensitivity) ------- COMMENT: 54db84e4ca91c654 184 ucVIx+e4w1j8wDaOgLtYTU5jGME (comment: CHECK chromium sensitivity) ------- COMMENT: 5524d2f4237ade3e 4 JIdk44KHLEmhWHdHQsw0uvHfOiM (comment: CHECK MEIOTIC !) Plo1 protein reorganize spindle body during meiosis: Plo1 starts to localize to spindle pole body at the onset of meiosis I, and recruits Cut12 (and Pcp1), which was absent during meiotic prophase. ------- COMMENT: 5524d2f4237ade3e 6 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 5524d2f4237ade3e 8 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 5524d2f4237ade3e 9 abo/L8CaHgD+2fMeR4wO/13O2ek Figure S6 ------- COMMENT: 5524d2f4237ade3e 10 abo/L8CaHgD+2fMeR4wO/13O2ek Figure S6 ------- COMMENT: 5524d2f4237ade3e 13 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 5524d2f4237ade3e 14 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 5524d2f4237ade3e 16 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 5524d2f4237ade3e 17 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 5524d2f4237ade3e 18 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 5524d2f4237ade3e 19 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 5524d2f4237ade3e 22 GhR/nSlpdB6oywaEl52+uUYamN4 Figure 4B ------- COMMENT: 5524d2f4237ade3e 26 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 5524d2f4237ade3e 28 qZES7TyNAgCzbCkiVHnIhigMjYg Figure 5A ------- COMMENT: 5524d2f4237ade3e 29 qZES7TyNAgCzbCkiVHnIhigMjYg Figure 5A ------- COMMENT: 5524d2f4237ade3e 30 qZES7TyNAgCzbCkiVHnIhigMjYg Figure 5A ------- COMMENT: 5524d2f4237ade3e 31 rDR41po8gfpi5g9cNpYWWk5easQ Figure 5 ------- COMMENT: 5524d2f4237ade3e 32 rDR41po8gfpi5g9cNpYWWk5easQ Figure 5 ------- COMMENT: 5524d2f4237ade3e 33 baeVYdGNtGQBb7AgxaKiSAKWYJE Figure 5F ------- COMMENT: 5524d2f4237ade3e 34 vmOMawZCAry1CcMYTVX/QAIV8u0 Figure 6A ------- COMMENT: 5524d2f4237ade3e 35 vmOMawZCAry1CcMYTVX/QAIV8u0 Figure 6A ------- COMMENT: 554b67537d04311a 12 mwuZeO8eospRht+eZIYIR8uMUHI Figure 1E ------- COMMENT: 554b67537d04311a 15 mwuZeO8eospRht+eZIYIR8uMUHI Figure 1E ------- COMMENT: 554b67537d04311a 16 JJ61Rr+BzZ+yYkS4ndaMB26Ouxc Figure 2B ------- COMMENT: 554b67537d04311a 17 i8xjStRjUf86+fPsXlMaA65Ocvs The preRC- loading delay was abolished in the irradiated gcn1Δ cells (Fig 3C) ------- COMMENT: 554b67537d04311a 18 i8xjStRjUf86+fPsXlMaA65Ocvs The preRC- loading delay was abolished in the irradiated gcn1Δ cells (Fig 3C) ------- COMMENT: 554b67537d04311a 21 S0n9fw83XA5jsPKfTmKai5TIxpo (comment: unfortunately no direct binding data, but physical interactions have been shown in other organisms) ------- COMMENT: 55601848bf58a3e9 2 8WUDGvue/Zu1zDHdBzwKN61tghI punctate, similar to nuclear pore components (comment: localization not dependent on microtubules) ------- COMMENT: 55601848bf58a3e9 6 gurfUVaYcFLoIcdYvON+DYWk0RI (comment: assayed using NLS-LacI-GFP construct) ------- COMMENT: 5564a30a271f4dce 1 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 558b22ef42aa6ec2 8 gWoNHE8D0zkcQt/aWkJ7tvGHflU fig 2D (comment: CHECK unattached kinetochore nda3-KM311 arrested cell) ------- COMMENT: 558b22ef42aa6ec2 16 0Ss+bfd/awEm8gSvsV0DkLRj0vo fig 3A,B ------- COMMENT: 558b22ef42aa6ec2 17 YBFBut5qi9nGLEjnRFEx94qv4ks fig 3b ------- COMMENT: 558b22ef42aa6ec2 18 9eIl2iWIJWroQ3R444DpXYYbDWk fig 2d ------- COMMENT: 558b22ef42aa6ec2 19 8C2CxQDRJ3iEzOSz1ApVX9MQHcg Fig. 2b (comment: mad1 localizes to unattached kinetochores) and fig 3a ------- COMMENT: 558b22ef42aa6ec2 20 mryEKeamiiFsLauzGAd/eLfktr4 fig 5C ------- COMMENT: 558b22ef42aa6ec2 21 tZO9udlIjKQSlnHG2rljncGTfBk fig2a (comment:diminished relocation from kinetochore) ------- COMMENT: 558b22ef42aa6ec2 27 uOwXOJ72Xu4BY6+2L2iOQ/Pbn1g (comment: kinetochore localization of Cut7 is unaffected) ------- COMMENT: 558b22ef42aa6ec2 33 U/PvCZ3/sYCTc+VkXd+IsT2uQcI fig1b ------- COMMENT: 558b22ef42aa6ec2 34 U/PvCZ3/sYCTc+VkXd+IsT2uQcI fig1b ------- COMMENT: 558b22ef42aa6ec2 35 U/PvCZ3/sYCTc+VkXd+IsT2uQcI fig1b ------- COMMENT: 558b22ef42aa6ec2 36 U/PvCZ3/sYCTc+VkXd+IsT2uQcI fig1b ------- COMMENT: 558b22ef42aa6ec2 38 WIQobKVmOSwF4pnw3zyHiiYk2Lk fig1a ------- COMMENT: 558b22ef42aa6ec2 39 WIQobKVmOSwF4pnw3zyHiiYk2Lk fig1a ------- COMMENT: 558b22ef42aa6ec2 40 WIQobKVmOSwF4pnw3zyHiiYk2Lk fig1a ------- COMMENT: 558b22ef42aa6ec2 41 WIQobKVmOSwF4pnw3zyHiiYk2Lk fig1a ------- COMMENT: 558b22ef42aa6ec2 42 WIQobKVmOSwF4pnw3zyHiiYk2Lk fig1a ------- COMMENT: 558b22ef42aa6ec2 43 WIQobKVmOSwF4pnw3zyHiiYk2Lk fig1a ------- COMMENT: 558b22ef42aa6ec2 45 KCEnhsIw7N99i5dCU97nSmwve0U fig1e ------- COMMENT: 558b22ef42aa6ec2 46 MeZlJGfEXf4/Cc7D9Zqj/osZ4/U figure 2a ------- COMMENT: 558b22ef42aa6ec2 47 0+TRM7WzNn3prtI8kMDZh1EZOPE figure 2a ------- COMMENT: 558b22ef42aa6ec2 48 j8lx0cjXOogtvtAy6trm2XWxJ9Q fig2a ------- COMMENT: 558b22ef42aa6ec2 49 j8lx0cjXOogtvtAy6trm2XWxJ9Q fig2a ------- COMMENT: 558b22ef42aa6ec2 52 0Ss+bfd/awEm8gSvsV0DkLRj0vo fig 3 a/b ------- COMMENT: 558b22ef42aa6ec2 53 0Ss+bfd/awEm8gSvsV0DkLRj0vo fig 3 a/b ------- COMMENT: 558b22ef42aa6ec2 54 23A7gKYH++mwxpDaLPxFOcAYx6Y fig 3c ------- COMMENT: 558b22ef42aa6ec2 59 r119F9zD84pzKT1S9UL73CuC9VE (comment: ABOLISHED tetermerization) ------- COMMENT: 558b22ef42aa6ec2 60 yV4gis4XWVqP0QX7WKH27+00P7E (comment: ABOLISHED tetermerization) fig 4f ------- COMMENT: 558b22ef42aa6ec2 76 OE5JWHG0QOF5HJ9f0xFZWfND2E4 fig 5c (comment: CHECK "gliding" new GO term requested) ------- COMMENT: 558b22ef42aa6ec2 77 KCEnhsIw7N99i5dCU97nSmwve0U fig 1e ------- COMMENT: 55b9bee3eb3b5a49 1 X7aTm1UQvmJba3ITsa8J5e1ZIlI Figure 3. tas3-TAM Mutations Cause a Dramatic Loss of ura4+ Silencing at imr1 but Only a Modest Loss at otr1 ------- COMMENT: 55b9bee3eb3b5a49 2 1uEOcFf8lGNAEmweMU80cSD2nEU Figure 3. ------- COMMENT: 55b9bee3eb3b5a49 3 X7aTm1UQvmJba3ITsa8J5e1ZIlI Figure 3. tas3-TAM Mutations Cause a Dramatic Loss of ura4+ Silencing at imr1 but Only a Modest Loss at otr1 ------- COMMENT: 55b9bee3eb3b5a49 4 X7aTm1UQvmJba3ITsa8J5e1ZIlI Figure 3. tas3-TAM Mutations Cause a Dramatic Loss of ura4+ Silencing at imr1 but Only a Modest Loss at otr1 ------- COMMENT: 55b9bee3eb3b5a49 5 X7aTm1UQvmJba3ITsa8J5e1ZIlI Figure 3. tas3-TAM Mutations Cause a Dramatic Loss of ura4+ Silencing at imr1 but Only a Modest Loss at otr1 ------- COMMENT: 55b9bee3eb3b5a49 6 pOwgAoRIvgdjqxuI2Y2E49LhQgI Figure 3. To rule out that the observed silencing defects were due to instability of the mutant Tas3 proteins, we examined the levels of wild-type and mutant Tas3 protein by western blotting and found that the mutant proteins were expressed to similar levels as the wild-type protein (Figure 3C). ------- COMMENT: 55b9bee3eb3b5a49 7 tIF6fqExgREY1LDpp6oqF/9bVdg (Figure 3C) ------- COMMENT: 55b9bee3eb3b5a49 8 tIF6fqExgREY1LDpp6oqF/9bVdg (Figure 3C) ------- COMMENT: 55b9bee3eb3b5a49 9 tIF6fqExgREY1LDpp6oqF/9bVdg (Figure 3C) ------- COMMENT: 55b9bee3eb3b5a49 11 gAkZMb+d2w/XTTgx1/mj/WsripI We found that mutant proteins coimmunoprecipitated with Chp1 and FLAG-Ago1 with similar efficiency as the wild-type protein (Figures 4A and 4B, compare lane 1 with lanes 2–5). This result demonstrates that RITS complex formation is not affected in tas3-TAM mutants and that theCterminus of Tas3 is not involved in Chp1 or Ago1 binding (also shown in Partridge et al. [2007]). ------- COMMENT: 55b9bee3eb3b5a49 12 bUAw/3tfduzZBX6L8TsQSPNVgwc To determine the effect of tas3-TAM mutations on cen siRNAs levels, we performed northern blot analysis on RNAs isolated from wild-type, tas3D, and tas3-TAM mutant cells. We found a dramatic reduction in the levels of both total (Figure 4C) and Ago1-purified (Figure 4D) cen siRNAs in all tas3-TAM mutants compared to wild-type. ------- COMMENT: 55b9bee3eb3b5a49 13 bUAw/3tfduzZBX6L8TsQSPNVgwc To determine the effect of tas3-TAM mutations on cen siRNAs levels, we performed northern blot analysis on RNAs isolated from wild-type, tas3D, and tas3-TAM mutant cells. We found a dramatic reduction in the levels of both total (Figure 4C) and Ago1-purified (Figure 4D) cen siRNAs in all tas3-TAM mutants compared to wild-type. ------- COMMENT: 55b9bee3eb3b5a49 14 bUAw/3tfduzZBX6L8TsQSPNVgwc To determine the effect of tas3-TAM mutations on cen siRNAs levels, we performed northern blot analysis on RNAs isolated from wild-type, tas3D, and tas3-TAM mutant cells. We found a dramatic reduction in the levels of both total (Figure 4C) and Ago1-purified (Figure 4D) cen siRNAs in all tas3-TAM mutants compared to wild-type. ------- COMMENT: 55b9bee3eb3b5a49 15 bUAw/3tfduzZBX6L8TsQSPNVgwc To determine the effect of tas3-TAM mutations on cen siRNAs levels, we performed northern blot analysis on RNAs isolated from wild-type, tas3D, and tas3-TAM mutant cells. We found a dramatic reduction in the levels of both total (Figure 4C) and Ago1-purified (Figure 4D) cen siRNAs in all tas3-TAM mutants compared to wild-type. ------- COMMENT: 55b9bee3eb3b5a49 16 TwXQxiDDq0VavzuIMl3heVlBZ+0 At the dg and dh repeats, we found a consistent 2- to 3-fold reduction in mutant Tas3 occupancy compared to wild-type (Figure 5B, compare lanes 3–6 with lane 2; and Figure 5C). Also, consistent with the otr1R::ura4+ silencing data (Figure 3B), mutant Tas3-TAM proteins associated with the ura4+ insert at otr1R less efficiently than wild-type Tas3 (Figure 5D, compare lanes 3–6 with lane 2; and Figure 5E). In ------- COMMENT: 55b9bee3eb3b5a49 20 i+0Se3iTYf07ZCciQMRbCWGHZuI Surprisingly, we found no defect in H3K9me in tas3DTAM compared to wild-type cells at native centromeric repeats (dg1, imr1, imr2-1, or imr2-2) or the ura4+ inserts (for imr1R::ura4+, ------- COMMENT: 55f39e07322aab36 1 3lwfePaDfkl2dlp7w54U2pLlr4g (Figure 1) ------- COMMENT: 55f39e07322aab36 2 YgyBxGt0ZaWipPLb3xj7yYfeY5g (Figure 3B) ------- COMMENT: 55f39e07322aab36 3 YgyBxGt0ZaWipPLb3xj7yYfeY5g (Figure 3B) ------- COMMENT: 55f39e07322aab36 4 YgyBxGt0ZaWipPLb3xj7yYfeY5g (Figure 3B) ------- COMMENT: 55f39e07322aab36 5 YgyBxGt0ZaWipPLb3xj7yYfeY5g (Figure 3B) ------- COMMENT: 55f39e07322aab36 8 E5X0cYHQG6heJ1X3wqm3izO7frI (Figure 3A,B) ------- COMMENT: 55f39e07322aab36 9 E5X0cYHQG6heJ1X3wqm3izO7frI (Figure 3A,B) ------- COMMENT: 55f39e07322aab36 10 bQrAvw5v3pdeGEjTasNZSNQl2dw (Figure 6A) ------- COMMENT: 55f39e07322aab36 13 5Leuj0nQtQ/oH58v8XLkYXSDMog (Figure 6B) ------- COMMENT: 55f39e07322aab36 14 W8U2HOh5c9FHrXYcx0iyvAr6hdQ (Figure 7A) ------- COMMENT: 55f39e07322aab36 15 0jqT+Ni6KPjcehoXDgH41EpcQMU Figure 3B (comment: additive) ------- COMMENT: 55f39e07322aab36 16 riH6PuZeW9pd2w953irT9kPPwpE Figure 8 ------- COMMENT: 55f39e07322aab36 17 riH6PuZeW9pd2w953irT9kPPwpE Figure 8 ------- COMMENT: 55f39e07322aab36 18 I6XlejwSL57g7vU4YIJVBUfo/KE Figure 9A ------- COMMENT: 563b3ea3f2426460 1 rQbV3RzIF1QauMhn/Z8DBShvP6I (comment: CHECK activated_by(CHEBI:18420)| activated_by(CHEBI:29035)) ------- COMMENT: 563bfc6f26ecd62e 39 CDToYNhJF3vMhVe6qk4/sRLqX6I (comment: inferred from protein binding phenotypes) ------- COMMENT: 563bfc6f26ecd62e 40 CDToYNhJF3vMhVe6qk4/sRLqX6I (comment: inferred from protein binding phenotypes) ------- COMMENT: 564d70e29944c82f 3 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: 564d70e29944c82f 4 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: 564d70e29944c82f 5 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: 564d70e29944c82f 6 yyMNf1x4ofpAA8TPiE7bhUbV4SU fig 3b ------- COMMENT: 566579944fc22e68 1 mfg45E4dP21IcLfoyL5S4z3Ckrc We tested for an interaction between Mob1p and Sid2p using the yeast two-hybrid system. Sid2p and Mob1p specifically interacted with each other but not with control proteins (Figure 1a) ------- COMMENT: 566579944fc22e68 2 fpsrnZvlGF+oIS8/xmvAhmFNhxM We tested for an interaction between Mob1p and Sid2p using the yeast two-hybrid system. Sid2p and Mob1p specifically interacted with each other but not with control proteins (Figure 1a) Western blotting with anti-Myc antibodies detected Sid2p–13Myc as two bands that co-immunoprecipitated with GFP–Mob1p (Figure 1b). ------- COMMENT: 566579944fc22e68 3 Evy6jmPup23OdrZZT151F9dALJI Kinase activity was associated with 13Myc–Mob1p prepared from wild-type cells, but Mob1p-associated kinase activity was reduced in sid2-250 cells (Figure 1c). ------- COMMENT: 566579944fc22e68 4 S4qq56VfYM+y3y21xWcoHDcc69k suggesting that Mob1-associated kinase activity is probably due to Sid2p. ------- COMMENT: 566579944fc22e68 5 2zflEPkbygJ41rgKSuXBv9vKvMg To examine the function of Mob1p in vivo, we deleted the mob1 gene (see Supplementary material) and found that it was essential for growth. ------- COMMENT: 566579944fc22e68 6 LPqwLJJ/leI+A6CA0kLAS6nV3ls After shifting to the restrictive temperature, mob1-1 cells failed to perform cytokinesis, became multinucleate, and did not form septa(Figure 2a–b). ------- COMMENT: 566579944fc22e68 7 L9g0e6s7edIegriHsbiBqJhM+FU Staining for the myosin light chain Cdc4p and actin revealed mob1-1 cells formed normal actomyosin rings and medially placed patches (Figure 2c–e). ------- COMMENT: 566579944fc22e68 8 L9g0e6s7edIegriHsbiBqJhM+FU Staining for the myosin light chain Cdc4p and actin revealed mob1-1 cells formed normal actomyosin rings and medially placed patches (Figure 2c–e). ------- COMMENT: 566579944fc22e68 9 wWihlZZuOqsP2JTkXhxVzRPg2PE Examination of microtubules showed that mob1-1 cells form normal mitotic spindles (Figure 2f,g), and interphase arrays of microtubules. Thus, unlike S. cerevisiae mob1 mutants, S. pombe mob1 mutants do not have defects in mitotic exit, but are specifically defective in cytokinesis ------- COMMENT: 566579944fc22e68 10 9hi9SeE1+Ef5ZkOuqkL8HhVd2nM These results are consistent with Mob1p localizing to the cell division site at, or just before, the initiation of septum formation, and then leaving the division site before cell separation. T ------- COMMENT: 566579944fc22e68 11 qMNHp12Eh/phH4Nj6RCJ+O9D/dg The nuclear-associated spotlike localization of Mob1p suggested that it was a component of the SPB. To confirm SPB localization, we fixed and stained cells expressing GFP–Mob1p with an antitubulin antibody. GFP–Mob1p was localized at the ends of the mitotic spindle in cells undergoing mitosis, consistent with its localization to the SPB (Figure 3m). ------- COMMENT: 566579944fc22e68 12 smzSqcZKhHlxBv+OkltNpWwb8J0 In all of the sid mutants, sid1, sid2, spg1, sid4 cdc7,cdc11 and cdc14, GFP–Mob1p could not localize to themedial region (Figure 4a–c and data not shown) (vw cdc15-140 in figure legend must be a typo) ------- COMMENT: 566579944fc22e68 13 AbYKeHl/OyqNmNxdH/i6tzTT9BE In all of the sid mutants, sid1, sid2, spg1, sid4 cdc7,cdc11 and cdc14, GFP–Mob1p could not localize to themedial region (Figure 4a–c and data not shown) ------- COMMENT: 566579944fc22e68 14 AbYKeHl/OyqNmNxdH/i6tzTT9BE In all of the sid mutants, sid1, sid2, spg1, sid4 cdc7,cdc11 and cdc14, GFP–Mob1p could not localize to themedial region (Figure 4a–c and data not shown) ------- COMMENT: 566579944fc22e68 15 CRNdm2kvtYi8prGFjF3z8hymQyY GFP–Mob1p localization to the SPB was unaffected in sid1, sid2, spg1 and cdc14 mutants (Figure 4a and data not shown), ------- COMMENT: 566579944fc22e68 16 CRNdm2kvtYi8prGFjF3z8hymQyY GFP–Mob1p localization to the SPB was unaffected in sid1, sid2, spg1 and cdc14 mutants (Figure 4a and data not shown), ------- COMMENT: 566579944fc22e68 17 1mVUkMwkCcp4H7oR+ihXFStQrs0 SPB was unaffected in sid1, sid2, spg1 and cdc14 mutants (Figure 4a and data not shown), but was severely affected in sid4, cdc7 and cdc11 mutants. ------- COMMENT: 566579944fc22e68 18 1mVUkMwkCcp4H7oR+ihXFStQrs0 SPB was unaffected in sid1, sid2, spg1 and cdc14 mutants (Figure 4a and data not shown), but was severely affected in sid4, cdc7 and cdc11 mutants. ------- COMMENT: 566579944fc22e68 19 gSA82dn4u0s2LM8a+r154/BlWZI These results support the model that Mob1p, like Sid2p, functions downstream in the sid pathway. Consistent with this hypothesis, we found that Cdc7p localizes normally to the SPB in a mob1-1 temperature-sensitive mutant (Figure 4f). I ------- COMMENT: 5694c1e2978a9fd3 3 0YUfTiW19B71LjUhcGgrDzAeLJE (comment: we don't know if they germinate or not) ------- COMMENT: 5694c1e2978a9fd3 5 L9ySx4fW+0NPnoCbbaNLTKno0a8 (comment: recombinant hal3 not a strong inhibitor in vitro) ------- COMMENT: 5696e14c1250e01e 1 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: 5696e14c1250e01e 2 82vfRQiKcefEVIcGaXHJnwTU0Os figure 1c and Double staining using a combination of cdc13- and tubulin- specific antibodies showed that the dots corresponded exactly to the positions of the mitotic spindle poles (Fig. 3a, b) ------- COMMENT: 5696e14c1250e01e 3 BcMypYvwaLSjaZS/r7aYmOXmwYw figure2 ------- COMMENT: 5696e14c1250e01e 5 82vfRQiKcefEVIcGaXHJnwTU0Os figure 1c and Double staining using a combination of cdc13- and tubulin- specific antibodies showed that the dots corresponded exactly to the positions of the mitotic spindle poles (Fig. 3a, b) ------- COMMENT: 56ab0acf3aa326fc 1 ryK/dBuUkEroTQTNzkJKKdupG7w (comment: CHECK occurs_during G1 to G0 transition) ------- COMMENT: 56ab0acf3aa326fc 4 edScdURHZXJhoKeP6gLANMEazPI (comment: CHECK occurs_during G1 to Go transition) ------- COMMENT: 56ab0acf3aa326fc 12 Q0nGJe3E0us7EjBYA+ZRN6rCdmE (comment: CHECK move down to G1, nitrogen induced) ------- COMMENT: 56ab0acf3aa326fc 18 05rLRjLbi3G1kDULNKCDnYFSRYc (comment: homothallic h90) ------- COMMENT: 56ab0acf3aa326fc 20 05rLRjLbi3G1kDULNKCDnYFSRYc (comment: homothallic h90) ------- COMMENT: 56d74acb634ee730 1 rhJL68SAjDXKaub2ODNki/wKM7k Sen2, Sen34, and Sen54 endogenously tagged at their C termini locate to mitochondria, as indicated by the overlapping signal with the mitochondrion stain MitoView Blue (Fig. 5A). H ------- COMMENT: 56d74acb634ee730 2 rhJL68SAjDXKaub2ODNki/wKM7k Sen2, Sen34, and Sen54 endogenously tagged at their C termini locate to mitochondria, as indicated by the overlapping signal with the mitochondrion stain MitoView Blue (Fig. 5A). H ------- COMMENT: 56d74acb634ee730 3 rhJL68SAjDXKaub2ODNki/wKM7k Sen2, Sen34, and Sen54 endogenously tagged at their C termini locate to mitochondria, as indicated by the overlapping signal with the mitochondrion stain MitoView Blue (Fig. 5A). H ------- COMMENT: 56d74acb634ee730 4 c6u51VWwkdaALtntyjueezvGBIc By confocal microscopy, Sen2-mECitrine and Sen54- mECitrine signals were very weak in tom70Δ cells, with the residual signals located in the cytoplasm and some lo- cated on mitochondria (Fig. 5A). ------- COMMENT: 56d74acb634ee730 5 A1NnNnA2kJwk3ZiWBq+W2rfZ3tE the levels of Sen2-mECitrine and Sen54-mECitrine are re- duced in tom70Δ cells compared with wild type (Fig. 5B; Supplemental Fig. S9). We interpret these results to sup- port the hypothesis that S. pombe Tom70 functions in the mitochondrial localization of SEN subunits; when mitochondrial localization is not achieved, the SEN sub- units become unstable, although it is possible that SEN subunits are poorly expressed in tom70Δ cells. ------- COMMENT: 56d74acb634ee730 6 0nBEbFNzRZ4ge6GxPgzg2BrBNWs If S. pombe Tom70 is important for function and/or stability of the SEN complex, then deletion of S. pombe TOM70 would be expected to cause defects in pre- tRNA splicing. We used Northern analysis with probes complementary to the 5′ exons or introns of S. pombe tRNALeuCAA and tRNAProCGG. tom70Δ cells accumulate more intron-containing tRNALeuCAA and tRNAProCGG than wild-type cells (Fig. 5C), documenting a role for S. pombe Tom70 in pre-tRNA splicing. ------- COMMENT: 56d74acb634ee730 7 srID+BQgDZnqJaw0RLi4KAb/iSQ (comment: CHECK DECREASED TRNA SPLICING) If S. pombe Tom70 is important for function and/or stability of the SEN complex, then deletion of S. pombe TOM70 would be expected to cause defects in pre- tRNA splicing. We used Northern analysis with probes complementary to the 5′ exons or introns of S. pombe tRNALeuCAA and tRNAProCGG. tom70Δ cells accumulate more intron-containing tRNALeuCAA and tRNAProCGG than wild-type cells (Fig. 5C), documenting a role for S. pombe Tom70 in pre-tRNA splicing. ------- COMMENT: 57009ca6a21af022 3 HKJqeAB6jjqmlwjVFboQbyudIRw (comment: CHECK OLD SPB) ------- COMMENT: 5724d1814ea9f342 2 KaTCNXK+sQb7+TMrEeV4vwpvSIQ (comment: Strong phenotype in crosses with fus1∆) ------- COMMENT: 5724d1814ea9f342 5 vmGtpCRA836YqHjBjCQBRq1x/VY (comment: more severe phenotype when crossed to fus1delta) ------- COMMENT: 5724d1814ea9f342 10 hxZT4dOrGIj6fyoBriqIpNCvag0 (comment: more severe phenotype when crossed to fus1∆) ------- COMMENT: 5724d1814ea9f342 14 EIsP9IFXh/plaFtX2gfLDt7B3Gk (comment: phenotype more severe when crossed to fus1∆) ------- COMMENT: 5724d1814ea9f342 16 MQfrKFAdw/0fSwlOvk6NckG+Hx0 (comment: Wider localization at shmoo tip) ------- COMMENT: 5724d1814ea9f342 18 BfRAr9OIHYW2/IMzihint5ef0bM wider localization at the shmoo tip ------- COMMENT: 5724d1814ea9f342 24 ezDsR7lcFIGzJJk//PK9Kxe7osM (comment: stronger phenotype when crossed to fus1∆) ------- COMMENT: 5724d1814ea9f342 25 p++m+m6cwPISh4W7NuPAmnuzTQY (comment: wider distribution along shmoo tip) ------- COMMENT: 5724d1814ea9f342 26 3D3sPUS7J6aQExUcWc1sMhF7boo (comment: wider localization) ------- COMMENT: 5724d1814ea9f342 28 3D3sPUS7J6aQExUcWc1sMhF7boo (comment: wider localization) ------- COMMENT: 5724d1814ea9f342 29 3D3sPUS7J6aQExUcWc1sMhF7boo (comment: wider localization) ------- COMMENT: 5724d1814ea9f342 31 3D3sPUS7J6aQExUcWc1sMhF7boo (comment: wider localization) ------- COMMENT: 5724d1814ea9f342 37 3D3sPUS7J6aQExUcWc1sMhF7boo (comment: wider localization) ------- COMMENT: 57346d6578962342 16 qkHAVEKOuNqdm6hSPDK2pQLeVWQ allows stress-dependent activation of ctt1 and srx1 to the same extent as wild-type cells; however, it constitutively induces expression of gpd1 and hsp9 (Fig. 2A). ------- COMMENT: 57346d6578962342 17 qkHAVEKOuNqdm6hSPDK2pQLeVWQ allows stress-dependent activation of ctt1 and srx1 to the same extent as wild-type cells; however, it constitutively induces expression of gpd1 and hsp9 (Fig. 2A). ------- COMMENT: 57346d6578962342 18 cFUREb4UE19xw/oxY0gbU70jkVo the HA-Atf1.1M mutant was fully able to suppress the sensitivity to peroxides of strain atf1, expression of the HA-Atf1.10M and 11M mutants did not alleviate this phenotype (supplemental Fig. S1C). ------- COMMENT: 57346d6578962342 20 k5C1F9CMtl+0Zsvs1XwJ3+WCNH8 2A,cells expressing the hypophosphorylation mutant HA-Atf1.10M are not able to fully trigger the ctt1 and srx1 genes after H2O2 stress ------- COMMENT: 57346d6578962342 24 W1Z6zmd4Ihd9qWoZ70MAlHJ5Now expression of HA-Atf1.10D fully suppressed all stress defects of cells lacking Sty1 (Fig. 2C). ------- COMMENT: 57346d6578962342 25 uySJVpGcFEQbPlkpcxScaDGzDMk whereas the expression of all stress genes in cells expressing HAAtf1.10D was not altered by sty1 deletion. Concomitantly ------- COMMENT: 57346d6578962342 26 uySJVpGcFEQbPlkpcxScaDGzDMk whereas the expression of all stress genes in cells expressing HAAtf1.10D was not altered by sty1 deletion. Concomitantly ------- COMMENT: 57346d6578962342 27 uySJVpGcFEQbPlkpcxScaDGzDMk whereas the expression of all stress genes in cells expressing HAAtf1.10D was not altered by sty1 deletion. Concomitantly ------- COMMENT: 57346d6578962342 28 uySJVpGcFEQbPlkpcxScaDGzDMk whereas the expression of all stress genes in cells expressing HAAtf1.10D was not altered by sty1 deletion. Concomitantly ------- COMMENT: 57346d6578962342 30 cFUREb4UE19xw/oxY0gbU70jkVo the HA-Atf1.1M mutant was fully able to suppress the sensitivity to peroxides of strain atf1, expression of the HA-Atf1.10M and 11M mutants did not alleviate this phenotype (supplemental Fig. S1C). ------- COMMENT: 57346d6578962342 31 cFUREb4UE19xw/oxY0gbU70jkVo the HA-Atf1.1M mutant was fully able to suppress the sensitivity to peroxides of strain atf1, expression of the HA-Atf1.10M and 11M mutants did not alleviate this phenotype (supplemental Fig. S1C). ------- COMMENT: 57346d6578962342 33 XBfuxsioeSRQ/mKBXjXExQMEnQA atf1 expressing the mutant named HA-Atf1.6M, lacking sites 5 to 10 in Atf1, was as sensitive to growth on peroxide-containing plates as cells lacking Atf1. ------- COMMENT: 57346d6578962342 37 b5VSiqypuJAtZOFcw/Miw9+jUbo Concomitantly, although expression of HA-Atf1.10M was not able to suppress the sensitivity to peroxides of strain atf1 (supplemental Fig. S1C and Fig. 1G), expression of HAAtf1.10D alleviated this phenotype (Fig. 1G). ------- COMMENT: 57346d6578962342 38 Xwusg4vlL4Mg0/vfnP+ev3e0AOo Importantly, expression of the phospho-mimicking HA-Atf1.10D (Fig. 2B) or HA-Atf1.6D (Fig S3, C and D) bypasses the requirement for a MAP kinase in the transcription process, which questions the direct participation of the kinase in Pol II initiation and/or elongation. ------- COMMENT: 57346d6578962342 41 pRjiqxeC6mfeYNwAJt2rohMBk20 Strains expressing wild-type Atf1 or Atf1.7M or Atf1.7D mutants displayed the same patterns of tolerance to peroxides and activation of stress genes as the constitutive amino-terminally tagged versions (supplemental Fig S4, B and C). ------- COMMENT: 57346d6578962342 42 yeemavsrj1BvGnyydiXb6AjoVwQ (comment: CHECK ditto) ------- COMMENT: 57346d6578962342 43 yeemavsrj1BvGnyydiXb6AjoVwQ (comment: CHECK ditto) ------- COMMENT: 57346d6578962342 44 yeemavsrj1BvGnyydiXb6AjoVwQ (comment: CHECK ditto) ------- COMMENT: 57346d6578962342 45 yeemavsrj1BvGnyydiXb6AjoVwQ (comment: CHECK ditto) ------- COMMENT: 57346d6578962342 49 W2nIsJ/itlyygNN9XmkA3KrKECY Atf1.7M-HA are constitutively bound to the gpd1 and hsp9 promoters both before and after stress ------- COMMENT: 57346d6578962342 50 yeemavsrj1BvGnyydiXb6AjoVwQ (comment: CHECK ditto) ------- COMMENT: 57346d6578962342 52 DNv694G5NZUlUqrPVc+g1j/MfIY whether Atf1 binding to DNA is dependent on the presence of Pcr1; as shown in Fig. 4D, Atf1-GFP is not recruited to DNA in pcr1 cells, with the only exception of srx1 ------- COMMENT: 57346d6578962342 53 oyS4NoU1oJJNCmq8pc/6H0fJycw expression of the Sty1-independent Atf1.7D-HA mutant cannot bypass the absence of Pcr1, as shown by the lack of transcription of stress genes (Fig. 4C). ------- COMMENT: 57346d6578962342 54 yeemavsrj1BvGnyydiXb6AjoVwQ (comment: CHECK ditto) ------- COMMENT: 57346d6578962342 55 yeemavsrj1BvGnyydiXb6AjoVwQ (comment: CHECK ditto) ------- COMMENT: 57346d6578962342 58 3cL5tXI9wbubcQjL9mnaF8EFBhc that Pap1 is dispensable for the activation of gpd1 and hsp9 but required for ctt1 and srx1 (Fig. 5A) ------- COMMENT: 57346d6578962342 59 yeemavsrj1BvGnyydiXb6AjoVwQ (comment: CHECK ditto) ------- COMMENT: 57346d6578962342 60 l5Nohw/ocP08WEFFf0xTLGFGGnQ Regarding the role of Pap1 and Atf1 at these genes, ChIP analysis indicates that the stress-dependent recruitment of Atf1 to ctt1 and srx1 promoters is dependent on Pap1 (Fig. 5C). ------- COMMENT: 57346d6578962342 61 4E3ZuPu4I05v46rgWwxETxMyTVI Atf1 is constitutively bound to srx1 and ctt1 in strain trr1.... ------- COMMENT: 57346d6578962342 62 AHN4Nm4JuLjS3pOdPN4gcfy5VA4 ....whereas it is never recruited to these promoters in cells expressing Pap1.C523D (Fig. 5D). ------- COMMENT: 57346d6578962342 74 YACC+nNtCZgDNaooyz1QSI8wz8A As shown in Fig. 2B, the capacity of HA-Atf1.10M to activate hsp9 and gpd1 after stress imposition was abolished in the absence of Sty1, ------- COMMENT: 57346d6578962342 75 YACC+nNtCZgDNaooyz1QSI8wz8A As shown in Fig. 2B, the capacity of HA-Atf1.10M to activate hsp9 and gpd1 after stress imposition was abolished in the absence of Sty1, ------- COMMENT: 57346d6578962342 77 R+1SBzOjc350SSBWbfZ7XIkFFak (comment: vw, I deleted Caludias annotation by mistake when comparing to the older partially completed session by Laura, so adding back !) ------- COMMENT: 574df5dbdb6edf7f 2 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: 574df5dbdb6edf7f 3 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: 574df5dbdb6edf7f 4 dLMc2lN+y4Ufz2MhLOHEIjs6B2A Fig2A ------- COMMENT: 574df5dbdb6edf7f 5 b+00gXuAJVmG6MoHxSub6s9WxVw Figure 4C ------- COMMENT: 574df5dbdb6edf7f 8 jS5DyxzFQTTQoOIJVQEI+wMl27g Figure 5, A and B ------- COMMENT: 574df5dbdb6edf7f 12 +8KlaMrbC9Ein2bFXjX3ccRmUQw fig 5D ------- COMMENT: 574df5dbdb6edf7f 22 W8U2HOh5c9FHrXYcx0iyvAr6hdQ Figure 7A ------- COMMENT: 5776ed0daa755f60 1 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 5776ed0daa755f60 2 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 5776ed0daa755f60 3 h9HHod/zPC/zePDOi3sCxNb03rA (mimic nitrogen starvation response, When starved for nitrogen, on the other hand, the cells divide twice and then arrest at G1) At 4 h and 8 h after the shift to36 °C, the average cell length was reduced to 6.4 μm and6.2 μm, respectively, which were ∼50% decreases com-pared to wild-type cells (13.0 μm and 12.9 μm). Similar tsresults were obtained for tor2 -19. It is of note that, in contrast to the reduced length, the cell width remained constant in these mutant cells. ------- COMMENT: 5776ed0daa755f60 4 h9HHod/zPC/zePDOi3sCxNb03rA (mimic nitrogen starvation response, When starved for nitrogen, on the other hand, the cells divide twice and then arrest at G1) At 4 h and 8 h after the shift to36 °C, the average cell length was reduced to 6.4 μm and6.2 μm, respectively, which were ∼50% decreases com-pared to wild-type cells (13.0 μm and 12.9 μm). Similar tsresults were obtained for tor2 -19. It is of note that, in contrast to the reduced length, the cell width remained constant in these mutant cells. ------- COMMENT: 5776ed0daa755f60 5 h9HHod/zPC/zePDOi3sCxNb03rA (mimic nitrogen starvation response, When starved for nitrogen, on the other hand, the cells divide twice and then arrest at G1) At 4 h and 8 h after the shift to36 °C, the average cell length was reduced to 6.4 μm and6.2 μm, respectively, which were ∼50% decreases com-pared to wild-type cells (13.0 μm and 12.9 μm). Similar tsresults were obtained for tor2 -19. It is of note that, in contrast to the reduced length, the cell width remained constant in these mutant cells. ------- COMMENT: 5776ed0daa755f60 6 h9HHod/zPC/zePDOi3sCxNb03rA (mimic nitrogen starvation response, When starved for nitrogen, on the other hand, the cells divide twice and then arrest at G1) At 4 h and 8 h after the shift to36 °C, the average cell length was reduced to 6.4 μm and6.2 μm, respectively, which were ∼50% decreases com-pared to wild-type cells (13.0 μm and 12.9 μm). Similar tsresults were obtained for tor2 -19. It is of note that, in contrast to the reduced length, the cell width remained constant in these mutant cells. ------- COMMENT: 5776ed0daa755f60 9 t0577n5bRLIr+WdvGJV/p/VKtD8 70% of ts the tor2 -13 cells committed sexual development to ts form zygotes and spores (Fig. 3B,C) ------- COMMENT: 5776ed0daa755f60 13 zPC3lg8VVpXJux9ppZ83s068jQw (comment: CONDITION at 30 degrees) Tor1 becomes necessary for cell growth when Tor2 function is compromised. ------- COMMENT: 5776ed0daa755f60 14 2nA4hZvJzFQzI0BI0tOpKasFOuY (comment: CONDITION at 30 degrees) ------- COMMENT: 5776ed0daa755f60 15 1nzN/8LDT5JVuUUyU/TLhNp2r5U tor1∆tor2 -19 showed only the 2C peak, and no 1C peak appeared at 36 °C ------- COMMENT: 5776ed0daa755f60 18 B3ztohZijt4u98PeyW5SVRmMr5U As expected, these double mutants behaved the same as tor1∆rhb1+o/e cells, in which growth was restored under stress conditions (the fourth row). ------- COMMENT: 577d9d669e38039c 1 mSbtFbl77LLe8N/E8gNtHOYq+8w Figure 2 and Figure 2-figure supplement 1 ------- COMMENT: 577d9d669e38039c 4 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 577d9d669e38039c 7 VSgymbwwXe3gcmQX26TWikaWfx0 when SpHfl1 was overexpressed from a strong nmt1 promoter, the cytosolic signal of mYFP-SpAtg8 disappeared and the vacuole membrane localization of mYFP-SpAtg8 became much more conspicuous (Figure 1F). ------- COMMENT: 577d9d669e38039c 8 qLrCHlC6pGZJj9I0NmEVx9dbhmM Figure 1 (direct assay for vacuolar membrane) and Figure 1-figure supplement 1 (sequence feature evidence for transmembrane) ------- COMMENT: 577d9d669e38039c 10 WchFPMWQf87quWn9a9iwNWkKp/I Figures 2A and 2B; (comment: CHECK assayed_using cpy1) ------- COMMENT: 577d9d669e38039c 11 KVgqVf5uy0s5nITqfjGx/EJfIOY Figures 2C, 2D, and Figure 2—figure supplement 2 ------- COMMENT: 577d9d669e38039c 12 KVgqVf5uy0s5nITqfjGx/EJfIOY Figures 2C, 2D, and Figure 2—figure supplement 2 ------- COMMENT: 577d9d669e38039c 13 KVgqVf5uy0s5nITqfjGx/EJfIOY Figures 2C, 2D, and Figure 2—figure supplement 2 ------- COMMENT: 577d9d669e38039c 15 WchFPMWQf87quWn9a9iwNWkKp/I Figures 2A and 2B; (comment: CHECK assayed_using cpy1) ------- COMMENT: 577d9d669e38039c 16 KVgqVf5uy0s5nITqfjGx/EJfIOY Figures 2C, 2D, and Figure 2—figure supplement 2 ------- COMMENT: 577d9d669e38039c 17 KVgqVf5uy0s5nITqfjGx/EJfIOY Figures 2C, 2D, and Figure 2—figure supplement 2 ------- COMMENT: 577d9d669e38039c 18 KVgqVf5uy0s5nITqfjGx/EJfIOY Figures 2C, 2D, and Figure 2—figure supplement 2 ------- COMMENT: 577d9d669e38039c 19 PwpEVLRQjwCCoLVmTpEpReZY8Sk Figure 2—figure supplement 1D ------- COMMENT: 577d9d669e38039c 20 WtxKloYsrsip/Zbxz+MWbL0k3XA Figure 2D and Figure 2—figure supplement 2 ------- COMMENT: 577d9d669e38039c 21 3SY6yEiCxYGRb4n2uj0pP43Z87w Figure 2—figure supplement 2 ------- COMMENT: 577d9d669e38039c 23 VjAFKtFB8fVBJajv6q5HhzVtJSs Figures 4C and 4D; (comment: CHECK assayed_using cpy1) ------- COMMENT: 577d9d669e38039c 24 wf185u4DRDkg7Sbpsn3gLe6+2NY Figure 4E ------- COMMENT: 577d9d669e38039c 25 wf185u4DRDkg7Sbpsn3gLe6+2NY Figure 4E ------- COMMENT: 577d9d669e38039c 32 WtxKloYsrsip/Zbxz+MWbL0k3XA Figure 2D and Figure 2—figure supplement 2 ------- COMMENT: 577d9d669e38039c 33 WtxKloYsrsip/Zbxz+MWbL0k3XA Figure 2D and Figure 2—figure supplement 2 ------- COMMENT: 577d9d669e38039c 34 3SY6yEiCxYGRb4n2uj0pP43Z87w Figure 2—figure supplement 2 ------- COMMENT: 577d9d669e38039c 35 WtxKloYsrsip/Zbxz+MWbL0k3XA Figure 2D and Figure 2—figure supplement 2 ------- COMMENT: 577d9d669e38039c 36 WtxKloYsrsip/Zbxz+MWbL0k3XA Figure 2D and Figure 2—figure supplement 2 ------- COMMENT: 577d9d669e38039c 37 3SY6yEiCxYGRb4n2uj0pP43Z87w Figure 2—figure supplement 2 ------- COMMENT: 577d9d669e38039c 38 WtxKloYsrsip/Zbxz+MWbL0k3XA Figure 2D and Figure 2—figure supplement 2 ------- COMMENT: 577d9d669e38039c 39 9XX2bciTrpV8aMdqVS0r1qlUROo Figure 3—figure supplement 1 ------- COMMENT: 577d9d669e38039c 40 YiDryiNtEi2zcHC8LR6T5pmlDrw Figure 2—figure supplement 1A ------- COMMENT: 577d9d669e38039c 41 Eg8Pjh+di6QjK0rxTRzajtTLdhE Figures 2C, 2D, and Figure 2—figure supplement 2; (comment: same as either single mutant) ------- COMMENT: 577d9d669e38039c 42 Eg8Pjh+di6QjK0rxTRzajtTLdhE Figures 2C, 2D, and Figure 2—figure supplement 2; (comment: same as either single mutant) ------- COMMENT: 577d9d669e38039c 43 Eg8Pjh+di6QjK0rxTRzajtTLdhE Figures 2C, 2D, and Figure 2—figure supplement 2; (comment: same as either single mutant) ------- COMMENT: 577d9d669e38039c 44 3exs175sqyt07yP7MSvFiGkaDUY Figure 3—figure supplement 1A, 2B ------- COMMENT: 577d9d669e38039c 45 3exs175sqyt07yP7MSvFiGkaDUY Figure 3—figure supplement 1A, 2B ------- COMMENT: 577d9d669e38039c 46 3exs175sqyt07yP7MSvFiGkaDUY Figure 3—figure supplement 1A, 2B ------- COMMENT: 577d9d669e38039c 47 3exs175sqyt07yP7MSvFiGkaDUY Figure 3—figure supplement 1A, 2B ------- COMMENT: 577d9d669e38039c 48 3exs175sqyt07yP7MSvFiGkaDUY Figure 3—figure supplement 1A, 2B ------- COMMENT: 577d9d669e38039c 49 dmJBja6nJbYTo07WT9FlzIOMdxs Figure 3—figure supplement 1A ------- COMMENT: 577d9d669e38039c 50 AIV+/63WPS5PH3SLcd9o0WOU5ao Figure 3—figure supplement 1B ------- COMMENT: 577d9d669e38039c 51 ruI8CwjvUEcUppM3YvOVBMzPp0c Figure 3—figure supplement 2 ------- COMMENT: 577d9d669e38039c 52 ruI8CwjvUEcUppM3YvOVBMzPp0c Figure 3—figure supplement 2 ------- COMMENT: 577d9d669e38039c 53 o6DuBxRBiedDWAPpJmpdx12RYLM Figure 4—figure supplement 1B; ------- COMMENT: 577d9d669e38039c 54 3exs175sqyt07yP7MSvFiGkaDUY Figure 3—figure supplement 1A, 2B ------- COMMENT: 577d9d669e38039c 55 3exs175sqyt07yP7MSvFiGkaDUY Figure 3—figure supplement 1A, 2B ------- COMMENT: 577d9d669e38039c 56 3exs175sqyt07yP7MSvFiGkaDUY Figure 3—figure supplement 1A, 2B ------- COMMENT: 577d9d669e38039c 57 3exs175sqyt07yP7MSvFiGkaDUY Figure 3—figure supplement 1A, 2B ------- COMMENT: 577d9d669e38039c 58 Rha90SCUdyhyih6lYKg8PZuXkFM Figure 3—figure supplement 1B, 2B; assayed_using cpy1 ------- COMMENT: 577d9d669e38039c 59 Rha90SCUdyhyih6lYKg8PZuXkFM Figure 3—figure supplement 1B, 2B; assayed_using cpy1 ------- COMMENT: 577d9d669e38039c 60 Rha90SCUdyhyih6lYKg8PZuXkFM Figure 3—figure supplement 1B, 2B; assayed_using cpy1 ------- COMMENT: 577d9d669e38039c 61 Rha90SCUdyhyih6lYKg8PZuXkFM Figure 3—figure supplement 1B, 2B; assayed_using cpy1 ------- COMMENT: 577d9d669e38039c 62 ruI8CwjvUEcUppM3YvOVBMzPp0c Figure 3—figure supplement 2 ------- COMMENT: 577d9d669e38039c 63 ruI8CwjvUEcUppM3YvOVBMzPp0c Figure 3—figure supplement 2 ------- COMMENT: 577d9d669e38039c 64 ruI8CwjvUEcUppM3YvOVBMzPp0c Figure 3—figure supplement 2 ------- COMMENT: 577d9d669e38039c 65 o/LVdIxR3T9e9GwcbdKy0jT1DI4 Figure 4A; ------- COMMENT: 577d9d669e38039c 66 sVQUY9fl1IFWu7EfSHyrxkO450Y Figure 4—figure supplement 1C; ------- COMMENT: 577d9d669e38039c 67 o/LVdIxR3T9e9GwcbdKy0jT1DI4 Figure 4A; ------- COMMENT: 577d9d669e38039c 68 o6DuBxRBiedDWAPpJmpdx12RYLM Figure 4—figure supplement 1B; ------- COMMENT: 577d9d669e38039c 69 o6DuBxRBiedDWAPpJmpdx12RYLM Figure 4—figure supplement 1B; ------- COMMENT: 577d9d669e38039c 70 o/LVdIxR3T9e9GwcbdKy0jT1DI4 Figure 4A; ------- COMMENT: 577d9d669e38039c 71 o6DuBxRBiedDWAPpJmpdx12RYLM Figure 4—figure supplement 1B; ------- COMMENT: 577d9d669e38039c 72 o6DuBxRBiedDWAPpJmpdx12RYLM Figure 4—figure supplement 1B; ------- COMMENT: 577d9d669e38039c 73 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: 577d9d669e38039c 74 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: 577d9d669e38039c 75 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: 577d9d669e38039c 76 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: 577d9d669e38039c 77 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: 577d9d669e38039c 78 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: 577d9d669e38039c 79 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: 577d9d669e38039c 80 I7X16TOJMyjcVNHie4eKS9rGs9Y Figure 4C,D ------- COMMENT: 577d9d669e38039c 81 I7X16TOJMyjcVNHie4eKS9rGs9Y Figure 4C,D ------- COMMENT: 577d9d669e38039c 82 I7X16TOJMyjcVNHie4eKS9rGs9Y Figure 4C,D ------- COMMENT: 577d9d669e38039c 83 I7X16TOJMyjcVNHie4eKS9rGs9Y Figure 4C,D ------- COMMENT: 577d9d669e38039c 84 I7X16TOJMyjcVNHie4eKS9rGs9Y Figure 4C,D ------- COMMENT: 577d9d669e38039c 85 I7X16TOJMyjcVNHie4eKS9rGs9Y Figure 4C,D ------- COMMENT: 577d9d669e38039c 86 I7X16TOJMyjcVNHie4eKS9rGs9Y Figure 4C,D ------- COMMENT: 577d9d669e38039c 88 3exs175sqyt07yP7MSvFiGkaDUY Figure 3—figure supplement 1A, 2B ------- COMMENT: 577d9d669e38039c 89 3exs175sqyt07yP7MSvFiGkaDUY Figure 3—figure supplement 1A, 2B ------- COMMENT: 577d9d669e38039c 90 rACzBTxi8PrULXmwvYe3gyMKuJU Figure 3—figure supplement 1B, 2B; assayed__using cpy1 ------- COMMENT: 57ac88324e0b82e6 4 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 57ac88324e0b82e6 5 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 57ac88324e0b82e6 6 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 57ac88324e0b82e6 7 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 57ac88324e0b82e6 8 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 57ac88324e0b82e6 9 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 57ac88324e0b82e6 10 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 57ac88324e0b82e6 13 ykJrBaNK7EJ2IvxoBfiOGnIAxPI Figure 4; blt1∆/gef2∆ phenotype equivalent to blt1∆ or gef2∆ single mutants ------- COMMENT: 57ac88324e0b82e6 14 ykJrBaNK7EJ2IvxoBfiOGnIAxPI Figure 4; blt1∆/gef2∆ phenotype equivalent to blt1∆ or gef2∆ single mutants ------- COMMENT: 57ac88324e0b82e6 15 hOE0fB3P62UD7E4nSysxNRZgrjU Figure 2; blt1∆/gef2∆ phenotype equivalent to blt1∆ or gef2∆ single mutants ------- COMMENT: 57ac88324e0b82e6 16 G8UYUISZpHsF+7prXya2sqO3QWk Figure 3; blt1∆/gef2∆ phenotype equivalent to blt1∆ or gef2∆ single mutants ------- COMMENT: 57ac88324e0b82e6 17 Db73Wm2ERb/tdVwrnRebgAWafn4 blt1∆/gef2∆ phenotype equivalent to blt1∆ or gef2∆ single mutants ------- COMMENT: 57ac88324e0b82e6 19 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 57ac88324e0b82e6 20 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 57ac88324e0b82e6 21 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 57ac88324e0b82e6 22 hOE0fB3P62UD7E4nSysxNRZgrjU Figure 2; blt1∆/gef2∆ phenotype equivalent to blt1∆ or gef2∆ single mutants ------- COMMENT: 57ac88324e0b82e6 23 G8UYUISZpHsF+7prXya2sqO3QWk Figure 3; blt1∆/gef2∆ phenotype equivalent to blt1∆ or gef2∆ single mutants ------- COMMENT: 57ac88324e0b82e6 24 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 57ac88324e0b82e6 25 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 57ac88324e0b82e6 26 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 57ac88324e0b82e6 27 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 57ac88324e0b82e6 28 ykJrBaNK7EJ2IvxoBfiOGnIAxPI Figure 4; blt1∆/gef2∆ phenotype equivalent to blt1∆ or gef2∆ single mutants ------- COMMENT: 57ac88324e0b82e6 29 ykJrBaNK7EJ2IvxoBfiOGnIAxPI Figure 4; blt1∆/gef2∆ phenotype equivalent to blt1∆ or gef2∆ single mutants ------- COMMENT: 57ac88324e0b82e6 32 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 57d00be9a0ffdd97 2 QGsIrdKYh0PwBVE+nvrH2Enokkg figure 1A ------- COMMENT: 57d00be9a0ffdd97 3 QGsIrdKYh0PwBVE+nvrH2Enokkg figure 1A ------- COMMENT: 57d00be9a0ffdd97 4 QGsIrdKYh0PwBVE+nvrH2Enokkg figure 1A ------- COMMENT: 57d00be9a0ffdd97 6 QGsIrdKYh0PwBVE+nvrH2Enokkg figure 1A ------- COMMENT: 57d00be9a0ffdd97 7 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: 57d00be9a0ffdd97 8 QGsIrdKYh0PwBVE+nvrH2Enokkg figure 1A ------- COMMENT: 57d00be9a0ffdd97 9 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: 57d00be9a0ffdd97 12 wCKIU2fNV4jHxp+oGtjLIBT1eaQ figure S3A ------- COMMENT: 57d00be9a0ffdd97 13 wCKIU2fNV4jHxp+oGtjLIBT1eaQ figure S3A ------- COMMENT: 57d00be9a0ffdd97 14 wCKIU2fNV4jHxp+oGtjLIBT1eaQ figure S3A ------- COMMENT: 57d00be9a0ffdd97 15 wCKIU2fNV4jHxp+oGtjLIBT1eaQ figure S3A ------- COMMENT: 57d00be9a0ffdd97 16 wCKIU2fNV4jHxp+oGtjLIBT1eaQ figure S3A ------- COMMENT: 57d00be9a0ffdd97 17 wCKIU2fNV4jHxp+oGtjLIBT1eaQ figure S3A ------- COMMENT: 57d00be9a0ffdd97 18 wCKIU2fNV4jHxp+oGtjLIBT1eaQ figure S3A ------- COMMENT: 57d00be9a0ffdd97 19 wCKIU2fNV4jHxp+oGtjLIBT1eaQ figure S3A ------- COMMENT: 57d00be9a0ffdd97 21 e/mSIACL/DIGv4F5UJSGucPlvU8 figure S3A COULD ALSO ADD TO ANTISENS RPL402, BUT NOT ANNOTATED IN GENOME ------- COMMENT: 57d00be9a0ffdd97 22 exxsqb3f7uPu5GWnRfqck2q/2Ck figure S3A COULD ALSO ADD TO ANTISENS RPL402, BUT NOT ANNOTATED ------- COMMENT: 57d00be9a0ffdd97 23 exxsqb3f7uPu5GWnRfqck2q/2Ck figure S3A COULD ALSO ADD TO ANTISENS RPL402, BUT NOT ANNOTATED ------- COMMENT: 57d00be9a0ffdd97 24 PCmRtTkWVE/4ihPRt4YqjXfedKw The interaction between these proteins was abolished in the absence of red1 (Fig 3A), suggesting that Red1 physically links Mmi1 with the exosome. and Fig 4B The direct binding of Red1 with Rrp6 was also observed (Fig 4B). ------- COMMENT: 57d00be9a0ffdd97 25 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 57d00be9a0ffdd97 34 exxsqb3f7uPu5GWnRfqck2q/2Ck figure S3A COULD ALSO ADD TO ANTISENS RPL402, BUT NOT ANNOTATED ------- COMMENT: 57d00be9a0ffdd97 38 H0MnaHI1FiMjP1CYNeqjyYIN72Q figure 5A ------- COMMENT: 57d00be9a0ffdd97 39 KntnJkiXmLt+bu4ukaXFz0c82Wk figure 5B ------- COMMENT: 57d00be9a0ffdd97 40 KntnJkiXmLt+bu4ukaXFz0c82Wk figure 5B ------- COMMENT: 57d00be9a0ffdd97 41 PKKClEb5YvfUv3yoSQYBgiC/qvE figure 6A ------- COMMENT: 57d00be9a0ffdd97 42 QGsIrdKYh0PwBVE+nvrH2Enokkg figure 1A ------- COMMENT: 57d00be9a0ffdd97 43 H0MnaHI1FiMjP1CYNeqjyYIN72Q figure 5A ------- COMMENT: 58586cbbae636cdc 1 tF5rDqIY/23BXN+Wq6VkvrvzcVo Thus, Ago1 from fission yeast has "slicing" activity and can direct site-specific cleavage of RNA substrates via siRNA. ------- COMMENT: 58586cbbae636cdc 3 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 58586cbbae636cdc 4 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 58586cbbae636cdc 5 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 58586cbbae636cdc 6 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 58586cbbae636cdc 7 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 58586cbbae636cdc 8 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 58586cbbae636cdc 9 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 58586cbbae636cdc 10 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 58586cbbae636cdc 11 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 58586cbbae636cdc 12 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 58586cbbae636cdc 13 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 58586cbbae636cdc 14 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 58586cbbae636cdc 15 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 58586cbbae636cdc 16 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 58586cbbae636cdc 17 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 58586cbbae636cdc 18 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 58586cbbae636cdc 19 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 58586cbbae636cdc 20 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 5885cef5c51f3f2d 1 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 5885cef5c51f3f2d 2 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 5885cef5c51f3f2d 3 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 5885cef5c51f3f2d 4 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 5885cef5c51f3f2d 5 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 5885cef5c51f3f2d 6 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 5885cef5c51f3f2d 7 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 5885cef5c51f3f2d 8 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 5885cef5c51f3f2d 9 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 5885cef5c51f3f2d 10 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 5885cef5c51f3f2d 11 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 5885cef5c51f3f2d 12 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 5885cef5c51f3f2d 13 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 5885cef5c51f3f2d 14 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 5885cef5c51f3f2d 15 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 5885cef5c51f3f2d 16 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 5885cef5c51f3f2d 17 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 5885cef5c51f3f2d 18 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 5885cef5c51f3f2d 19 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 5885cef5c51f3f2d 20 roQ7Nb58jDobHCqYX+CwEviZmtw (comment: Small rescue of cut7D pkl1D) ------- COMMENT: 5885cef5c51f3f2d 23 hpV+eTbl1qQklv6XwaHqFimmwRQ (comment: CONDITION 5ug/mL) ------- COMMENT: 5885cef5c51f3f2d 24 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 58a02b795c025a88 1 jOkj4fW4Zlwl3vL9DWXjphkUkVU The drc1-191 mutation was found to be recessive, since cells of the genotype drc1􏰀/drc1- 191 resembled wild-type cells and were capable of colony formation under conditions in which the drc1-191 mu- tant was unable to form colonies (data not shown). ------- COMMENT: 58a02b795c025a88 2 jOkj4fW4Zlwl3vL9DWXjphkUkVU The drc1-191 mutation was found to be recessive, since cells of the genotype drc1􏰀/drc1- 191 resembled wild-type cells and were capable of colony formation under conditions in which the drc1-191 mu- tant was unable to form colonies (data not shown). ------- COMMENT: 58a02b795c025a88 3 l/ko/sNB50J9U397hTQxVZFTCKA Figure 1, 4 hr ------- COMMENT: 58a02b795c025a88 4 xp2wTbfp2iEfjvdK2DzuD4U9ilQ Figure 1, 8 hr ------- COMMENT: 58a02b795c025a88 5 S80YIbIMM92eJx94Oi0dxo4S+Qs Figure 1, 0 hr ------- COMMENT: 58a02b795c025a88 6 kdhYnKWxJ0IdYwnOSTVJ9Rs+jrY Microtubule staining confirmed that the drc1-191 cells were arrested in interphase since cells blocked predominantly either with interphase arrays of microtubules or with a postana- phase array of microtubules (Figure 3A, 4 hr). ------- COMMENT: 58a02b795c025a88 7 gNtV7ygl6vIHmpgq3WVGy/iws+8 Upon prolonged incubation at the restrictive temperature (Figure 2, 8 hr), cells assumed a variety of shapes and 􏰍20% of cells were found to contain four nuclei with actomyosin rings, whereas the rest of the cells (80%) still contained only two interphase nuclei and detectable ------- COMMENT: 58a02b795c025a88 8 gNtV7ygl6vIHmpgq3WVGy/iws+8 Upon prolonged incubation at the restrictive temperature (Figure 2, 8 hr), cells assumed a variety of shapes and 􏰍20% of cells were found to contain four nuclei with actomyosin rings, whereas the rest of the cells (80%) still contained only two interphase nuclei and detectable ------- COMMENT: 58a02b795c025a88 9 gNtV7ygl6vIHmpgq3WVGy/iws+8 Upon prolonged incubation at the restrictive temperature (Figure 2, 8 hr), cells assumed a variety of shapes and 􏰍20% of cells were found to contain four nuclei with actomyosin rings, whereas the rest of the cells (80%) still contained only two interphase nuclei and detectable ------- COMMENT: 58a02b795c025a88 10 gNtV7ygl6vIHmpgq3WVGy/iws+8 Upon prolonged incubation at the restrictive temperature (Figure 2, 8 hr), cells assumed a variety of shapes and 􏰍20% of cells were found to contain four nuclei with actomyosin rings, whereas the rest of the cells (80%) still contained only two interphase nuclei and detectable ------- COMMENT: 58a02b795c025a88 11 uEM6GuW27V1Y2NIY0FoCpC8DqNU (comment: i.e next round of replication) ------- COMMENT: 58a02b795c025a88 12 p5bdPwOAiLQ/wWfkg60eNvgkI3A At the drc1-191 arrest point all binucleate cells were found to have Cdc7p staining localized at one SPB. Merged images of chromosomal staining with DAPI and Cdc7p staining with HA antibodies is shown in Figure 4. The drc1-191 mutant, therefore, arrests at a point in the cell cycle where the septum-promoting Cdc7p is located on one SPB. ------- COMMENT: 58a02b795c025a88 13 q3MNFNX83jMUfflcyapfQKFaT2Q capable of germination and establishing polarized growth, but were incapable of performing cytokinesis and did not maintain polarity (Figure 6B). ------- COMMENT: 58a02b795c025a88 14 q3MNFNX83jMUfflcyapfQKFaT2Q capable of germination and establishing polarized growth, but were incapable of performing cytokinesis and did not maintain polarity (Figure 6B). ------- COMMENT: 58a02b795c025a88 15 q3MNFNX83jMUfflcyapfQKFaT2Q capable of germination and establishing polarized growth, but were incapable of performing cytokinesis and did not maintain polarity (Figure 6B). ------- COMMENT: 58a02b795c025a88 16 KwCxnNSKq6NG4IDzIBzzctmJHg4 Germinated drc1::ura4 spores were capable of po- larity establishment (shown with arrows in Figure 6C), but appeared to be incapable of polarity maintenance, causing them to become spherical and highly enlarged (Figure 6C). ------- COMMENT: 58a02b795c025a88 17 x7wuKD5VBZA3uC/7bdYZ6k6dOf0 Interestingly, unlike the drc1-191 mutant, drc1::ura4 underwent multiple nuclear division cycles causing arrested cells to accumulate up to 32 nuclei. ------- COMMENT: 58a02b795c025a88 18 uEM6GuW27V1Y2NIY0FoCpC8DqNU (comment: i.e next round of replication) ------- COMMENT: 58a02b795c025a88 19 Yw8AELPULAUMeziNApN17tU1h+k The drc1-191 myo2-E1 double mutant was unable to form colonies at 24􏰌, a temperature at which both parental strains were capable of colony formation (data not shown). ------- COMMENT: 58a02b795c025a88 20 VcxGCxPVMfewXAJ8CubBmzYt6zA The drc1-191 rng2-D5 and drc1-191 cdc4-8 double mutants grew extremely poorly and showed cyto- kinesis defects at 24􏰌, a temperature at which rng2-D5 and cdc4-8 single mutants grew healthily and resembled wild-type cells in morphology (Figure 7). In both double mutant combinations (drc1-191 cdc4-8 and drc1-191 rng2- D5) highly elongated cells with multiple nuclei were seen frequently. ------- COMMENT: 58a02b795c025a88 21 ytFeibJkeHksihHWNPoGuDIJ3Ho The drc1-191 rng2-D5 and drc1-191 cdc4-8 double mutants grew extremely poorly and showed cyto- kinesis defects at 24􏰌, a temperature at which rng2-D5 and cdc4-8 single mutants grew healthily and resembled wild-type cells in morphology (Figure 7). ------- COMMENT: 58a02b795c025a88 22 vHJy2JRJ5euHF18cV4e8wbnzhow The drc1-191 rng2-D5 and drc1-191 cdc4-8 double mutants grew extremely poorly a ------- COMMENT: 58d4c8cee7e46725 1 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 58d4c8cee7e46725 2 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 58d4c8cee7e46725 3 ouUrCWnsvC8kjaXbHgyZWSayPb8 Fig. 1B oreover, actin cables often significantly overgrew in these cells while the actin ring formation seemed to be unaf- fected. ------- COMMENT: 58d4c8cee7e46725 4 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 58d4c8cee7e46725 7 d2xhU+BwKEA4CRJroIGqoHtAa08 (comment: CHECK formation) ------- COMMENT: 58d4c8cee7e46725 12 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 58d4c8cee7e46725 13 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 58d4c8cee7e46725 15 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 58e0190b91ae4c1c 27 Ks4ebLkutZ3MmOrqfF3zq9XMoz4 as cyclin-CDK complex with Cdc13 or Cig2 ------- COMMENT: 59258724594b2b0c 28 oTfAdJPmzFMOaB6ECRUN5WtISpk (comment: CHECK would it be better/safer to annotate to parent generic intra-S checkpoint term?) ------- COMMENT: 593e1f8fb43caaa9 1 7611l+SWg0XBlhz4EGKibRbmyko (comment: alkaline DNA preparation) ------- COMMENT: 593e1f8fb43caaa9 2 7611l+SWg0XBlhz4EGKibRbmyko (comment: alkaline DNA preparation) ------- COMMENT: 59469ef8c399b1f8 1 mz/js6UP1sVrWJQcb/qkQCS13oc (comment: Use of Western blot to assay phosphorylation levels) ------- COMMENT: 59469ef8c399b1f8 2 Cw6fN2Qz2ULzyh/7lbVt96NNwWI The checkpoint doesn't sense all types of dna damage eg that caused by gamma radiation or DNA adduct formation by PUVA ------- COMMENT: 59904c3249cbb80b 1 TRvTzRQ3AWJTHQ/Nh03BxI11aR4 Fig. 1. ------- COMMENT: 59904c3249cbb80b 2 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 59904c3249cbb80b 3 hs/onUx0kUSBum/ufnp78JugzmI ------- COMMENT: 59904c3249cbb80b 4 nWe8j8bcR3HuTt7Sh/rO18fQBP8 Fig. 2. ------- COMMENT: 59904c3249cbb80b 5 X/ODnH+11XrD+CLy15WaDbDWEgg Fig. 2. (comment: CHECK It's only a bit worse (+3% chromosome loss). Not sure if worth including) ------- COMMENT: 59904c3249cbb80b 6 BMHQos46WbYiPfQdcLplFjQEFEQ msd1D and double-deletion pkl1D msd1D both showed similar rates of minichromosome loss compared with pkl1D (Fig. 1f), with msd1D at 8.2% and pkl1D msd1D at 11%) ------- COMMENT: 59904c3249cbb80b 7 YuRdk2KLxEbF5WdzUQha9+Xh/5g Fig. S2 Pkl1md-GFP localized primarily to the spindle poles and almost completely rescued the protrusion phenotype ------- COMMENT: 59904c3249cbb80b 8 lgEj8CMJtKMQrvzpfStUg4Q2Ik0 Fig. S2 In contrast, in pkl1D msd1D cells, Pkl1md-GFP localized primarily to the spindle and only partially rescued the protrusion phenotype ------- COMMENT: 59904c3249cbb80b 9 Iw+bzB4agINds1I6iroUkAVVchw Fig. S2 Pkl1md-GFP localized primarily to the spindle poles ------- COMMENT: 59904c3249cbb80b 10 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 59904c3249cbb80b 11 Wet7w4o5rU1SHq2KE9BQQTpVBKI Fig. 3 This is important as it indicates that the delay in pkl1D is likely not due to a delay in chromosome capture, but rather spindle formation in prophase. ------- COMMENT: 59904c3249cbb80b 12 EERHYdhBh5fV76bOVOpgj+gg4jU Fig. 3 MT buckling during prolonged contact with the cell tip cortex—its associated chromosome mass to the medial cell division site (Fig. 3c). Subsequent cytokinesis appeared to cut through the chromosome mass, resulting in aneuploidy in 12% of mitotic cells. ------- COMMENT: 59904c3249cbb80b 13 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 59904c3249cbb80b 14 bT7fI6CLBeFzytg+qXV5q17gSD8 Rescue of long protrusions Fig. 4 ------- COMMENT: 59904c3249cbb80b 15 5GTySTxW564o6mlCik+gb7FCHzM Rescue of long protrusions Fig. S4 ------- COMMENT: 59904c3249cbb80b 16 nR4e2wk8pJEuGjqjBTQmwgD09TQ Fig. 4e (comment: control missing so no phenotype annotation) ------- COMMENT: 59904c3249cbb80b 17 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 1 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 59bf3609c95bb937 2 +yrJ8bzLu+Y+QMiNKAZXPi+vBZE Figs. 1A, 5A, 6A, 7A, 8A ------- COMMENT: 59bf3609c95bb937 3 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 59bf3609c95bb937 4 6MREhTy2+tAO1iFmHRZR98fyORE Figs. 1B, 5B, 6B, 7B, 8B, 9B, 10B ------- COMMENT: 59bf3609c95bb937 5 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 59bf3609c95bb937 6 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 59bf3609c95bb937 7 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 59bf3609c95bb937 8 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 59bf3609c95bb937 9 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 59bf3609c95bb937 10 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 59bf3609c95bb937 12 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 59bf3609c95bb937 13 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 59bf3609c95bb937 14 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 59bf3609c95bb937 15 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 59bf3609c95bb937 16 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 59bf3609c95bb937 17 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 59bf3609c95bb937 18 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 59bf3609c95bb937 19 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 59bf3609c95bb937 20 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 59bf3609c95bb937 21 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 59bf3609c95bb937 22 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 59bf3609c95bb937 23 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 59bf3609c95bb937 24 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 59bf3609c95bb937 25 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 59bf3609c95bb937 26 y3ZWtz4jKCYO0r16IAD8yV0s9Kk Fig. 6A, 10A ------- COMMENT: 59bf3609c95bb937 27 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 59bf3609c95bb937 28 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 59bf3609c95bb937 29 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 59bf3609c95bb937 30 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 59bf3609c95bb937 31 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 59bf3609c95bb937 32 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 59bf3609c95bb937 33 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 59bf3609c95bb937 34 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 59bf3609c95bb937 35 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 59bf3609c95bb937 36 0qQCnM6jHw9XTeFvG7AOB5nAY9A Fig. 7B, 10B, 12B ------- COMMENT: 59bf3609c95bb937 37 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 59bf3609c95bb937 38 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 59bf3609c95bb937 39 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 59bf3609c95bb937 40 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 59bf3609c95bb937 41 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 59bf3609c95bb937 42 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 59bf3609c95bb937 43 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 59bf3609c95bb937 44 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 59bf3609c95bb937 45 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 59bf3609c95bb937 46 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 59bf3609c95bb937 47 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 59bf3609c95bb937 48 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 59bf3609c95bb937 49 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 59bf3609c95bb937 50 JUKiF37j64aMaKpSH0OBgfVgnXQ Fig. 8A ------- COMMENT: 59bf3609c95bb937 51 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: 59bf3609c95bb937 52 JUKiF37j64aMaKpSH0OBgfVgnXQ Fig. 8A ------- COMMENT: 59bf3609c95bb937 53 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: 59bf3609c95bb937 54 FkWjCHPXelPQLtr1AkZdsc+Y128 Fig. 9A ------- COMMENT: 59bf3609c95bb937 55 FkWjCHPXelPQLtr1AkZdsc+Y128 Fig. 9A ------- COMMENT: 59bf3609c95bb937 56 FkWjCHPXelPQLtr1AkZdsc+Y128 Fig. 9A ------- COMMENT: 59bf3609c95bb937 57 FkWjCHPXelPQLtr1AkZdsc+Y128 Fig. 9A ------- COMMENT: 59bf3609c95bb937 58 FkWjCHPXelPQLtr1AkZdsc+Y128 Fig. 9A ------- COMMENT: 59bf3609c95bb937 59 FkWjCHPXelPQLtr1AkZdsc+Y128 Fig. 9A ------- COMMENT: 59bf3609c95bb937 60 FkWjCHPXelPQLtr1AkZdsc+Y128 Fig. 9A ------- COMMENT: 59bf3609c95bb937 61 h+O7MFsPEg71zf/rtaxivJNoU7g Fig. 9B ------- COMMENT: 59bf3609c95bb937 62 h+O7MFsPEg71zf/rtaxivJNoU7g Fig. 9B ------- COMMENT: 59bf3609c95bb937 63 h+O7MFsPEg71zf/rtaxivJNoU7g Fig. 9B ------- COMMENT: 59bf3609c95bb937 64 h+O7MFsPEg71zf/rtaxivJNoU7g Fig. 9B ------- COMMENT: 59bf3609c95bb937 65 h+O7MFsPEg71zf/rtaxivJNoU7g Fig. 9B ------- COMMENT: 59bf3609c95bb937 66 h+O7MFsPEg71zf/rtaxivJNoU7g Fig. 9B ------- COMMENT: 59bf3609c95bb937 67 h+O7MFsPEg71zf/rtaxivJNoU7g Fig. 9B ------- COMMENT: 59bf3609c95bb937 68 ZfF+TnldqmeZGktILiSCRZ5t0KU Fig. 10A ------- COMMENT: 59bf3609c95bb937 69 ZfF+TnldqmeZGktILiSCRZ5t0KU Fig. 10A ------- COMMENT: 59bf3609c95bb937 70 ZfF+TnldqmeZGktILiSCRZ5t0KU Fig. 10A ------- COMMENT: 59bf3609c95bb937 71 ZfF+TnldqmeZGktILiSCRZ5t0KU Fig. 10A ------- COMMENT: 59bf3609c95bb937 72 iAc0LJPk9oNGqHXYM8jvzw5W7jY Fig. 10B ------- COMMENT: 59bf3609c95bb937 73 iAc0LJPk9oNGqHXYM8jvzw5W7jY Fig. 10B ------- COMMENT: 59bf3609c95bb937 75 7MScTZIOYuQE37aLF2/65qlG3dM Fig. 11A ------- COMMENT: 59bf3609c95bb937 76 HDuJbekp6v7XGVPSxVr7Gw93NCU Fig. 11B ------- COMMENT: 59bf3609c95bb937 77 HDuJbekp6v7XGVPSxVr7Gw93NCU Fig. 11B ------- COMMENT: 59bf3609c95bb937 78 TIz2iUixtSqYNErKe7XhRQ231q0 Fig. 12A ------- COMMENT: 59bf3609c95bb937 79 rJk8iPADuKsLWZd+J38CHH5tqqM Fig. 12B ------- COMMENT: 59bf3609c95bb937 80 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 59bf3609c95bb937 81 h+O7MFsPEg71zf/rtaxivJNoU7g Fig. 9B ------- COMMENT: 59bf3609c95bb937 82 TIz2iUixtSqYNErKe7XhRQ231q0 Fig. 12A ------- COMMENT: 59bf3609c95bb937 83 rJk8iPADuKsLWZd+J38CHH5tqqM Fig. 12B ------- COMMENT: 59bf3609c95bb937 84 TIz2iUixtSqYNErKe7XhRQ231q0 Fig. 12A ------- COMMENT: 59bf3609c95bb937 85 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 86 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 87 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 88 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 89 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 90 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 91 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 92 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 93 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 94 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 95 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 96 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 97 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 98 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 99 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 100 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 101 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 102 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 103 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 104 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 105 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 106 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 107 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 59bf3609c95bb937 108 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 59bf3609c95bb937 109 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 59bf3609c95bb937 110 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 59bf3609c95bb937 111 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 59bf3609c95bb937 112 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 59bf3609c95bb937 113 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 59bf3609c95bb937 114 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 59bf3609c95bb937 115 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 59bf3609c95bb937 116 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 59bf3609c95bb937 117 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 59bf3609c95bb937 118 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 59bf3609c95bb937 119 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 59bf3609c95bb937 120 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 59bf3609c95bb937 121 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 59bf3609c95bb937 122 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 59bf3609c95bb937 123 h8WWDxqyE11+2Ku4VRtm9AbY22k Fig. S5A ------- COMMENT: 59bf3609c95bb937 124 Zq2Qgvwbtq3yBSVmITg7uCIJC9M Fig. S5B ------- COMMENT: 59bf3609c95bb937 125 s0LMevRjNsm+dxiJbpnwPvFignE Fig. S6A ------- COMMENT: 59bf3609c95bb937 126 s0LMevRjNsm+dxiJbpnwPvFignE Fig. S6A ------- COMMENT: 59bf3609c95bb937 127 ZSoIdAAkba/kthzeLNn7KxqZ/Bw Fig. S6B ------- COMMENT: 59bf3609c95bb937 128 UYdl30f2oAnKjj3Tfq8Yt6o95Mg Fig. S7A ------- COMMENT: 59bf3609c95bb937 129 UYdl30f2oAnKjj3Tfq8Yt6o95Mg Fig. S7A ------- COMMENT: 59bf3609c95bb937 130 UYdl30f2oAnKjj3Tfq8Yt6o95Mg Fig. S7A ------- COMMENT: 59bf3609c95bb937 131 +D2eQH/P5QV9SHYuOwDKxxcxHPQ Fig. S7B ------- COMMENT: 59bf3609c95bb937 132 +D2eQH/P5QV9SHYuOwDKxxcxHPQ Fig. S7B ------- COMMENT: 59bf3609c95bb937 133 +D2eQH/P5QV9SHYuOwDKxxcxHPQ Fig. S7B ------- COMMENT: 59bf3609c95bb937 134 7fbuic9J4nxECAcRt8dTP1kf/5Q Fig. S8 ------- COMMENT: 59bf3609c95bb937 135 7fbuic9J4nxECAcRt8dTP1kf/5Q Fig. S8 ------- COMMENT: 59bf3609c95bb937 136 7fbuic9J4nxECAcRt8dTP1kf/5Q Fig. S8 ------- COMMENT: 59bf3609c95bb937 137 7fbuic9J4nxECAcRt8dTP1kf/5Q Fig. S8 ------- COMMENT: 59bf3609c95bb937 138 7fbuic9J4nxECAcRt8dTP1kf/5Q Fig. S8 ------- COMMENT: 59bf3609c95bb937 139 7fbuic9J4nxECAcRt8dTP1kf/5Q Fig. S8 ------- COMMENT: 59bf3609c95bb937 140 wTaRGJRcu5Oh8YVyLxaOI0Rm5IU Fig. 1A. Redundancy with rpb1-T4A ------- COMMENT: 59bf3609c95bb937 141 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 59bf3609c95bb937 142 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 59bf3609c95bb937 143 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 59bf3609c95bb937 144 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 59bf3609c95bb937 153 FkWjCHPXelPQLtr1AkZdsc+Y128 Fig. 9A ------- COMMENT: 59bf3609c95bb937 154 dr3tsDQrZJRdZihrhMLdURneSKU Fig. 13 ------- COMMENT: 59bf3609c95bb937 156 dr3tsDQrZJRdZihrhMLdURneSKU Fig. 13 ------- COMMENT: 59bf3609c95bb937 157 dr3tsDQrZJRdZihrhMLdURneSKU Fig. 13 ------- COMMENT: 59d6007b5a42a1e8 2 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 59d6007b5a42a1e8 3 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 59d6007b5a42a1e8 4 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 59d6007b5a42a1e8 5 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 59d6007b5a42a1e8 6 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 59d6007b5a42a1e8 7 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 59d6007b5a42a1e8 8 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: 59d6007b5a42a1e8 9 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: 59d6007b5a42a1e8 10 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: 59d6007b5a42a1e8 11 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: 59d6007b5a42a1e8 12 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: 59d6007b5a42a1e8 13 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: 59d6007b5a42a1e8 14 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 59d6007b5a42a1e8 15 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 59d6007b5a42a1e8 16 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 59d6007b5a42a1e8 17 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 59d6007b5a42a1e8 18 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: 59d6007b5a42a1e8 20 4NYxgkXa0C3lu7B63+ioQFKD5PQ (Figs. 1B and 2B) ------- COMMENT: 59d6007b5a42a1e8 21 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 59d6007b5a42a1e8 22 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 59d6007b5a42a1e8 24 6xFTmQSlBq6DnboXdFygslNMHFM Figs. 5 and 6 ------- COMMENT: 59d6007b5a42a1e8 25 6xFTmQSlBq6DnboXdFygslNMHFM Figs. 5 and 6 ------- COMMENT: 59d6007b5a42a1e8 26 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 59d6007b5a42a1e8 27 hkhnJyjrlYZCJMqKEHMrFDczBik Siw14 acts on 1,5-IP8, but it has no preference for either the 5 or 1-beta phosphates (Figs.5 and 6) ------- COMMENT: 59d6007b5a42a1e8 28 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 59d6007b5a42a1e8 29 t0t7USa0WJdf1mop/t+07fnJ5YI Fig. S1. ------- COMMENT: 59d6007b5a42a1e8 30 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 59d6007b5a42a1e8 31 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 59d6007b5a42a1e8 32 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 59d6007b5a42a1e8 33 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 59d6007b5a42a1e8 34 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 59d6007b5a42a1e8 35 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 59d6007b5a42a1e8 36 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 59d6007b5a42a1e8 37 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 59d6007b5a42a1e8 38 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 59d6007b5a42a1e8 39 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 59d6007b5a42a1e8 40 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 59d6007b5a42a1e8 41 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 59d6007b5a42a1e8 42 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 59d6007b5a42a1e8 43 hkhnJyjrlYZCJMqKEHMrFDczBik Siw14 acts on 1,5-IP8, but it has no preference for either the 5 or 1-beta phosphates (Figs.5 and 6) ------- COMMENT: 5a0095c9d74677e3 20 FYYD4chDCAD0gnyp/2G1wu1g5lA (comment: like dis1-288 alone) ------- COMMENT: 5a0095c9d74677e3 21 FYYD4chDCAD0gnyp/2G1wu1g5lA (comment: like dis1-288 alone) ------- COMMENT: 5a010480a9612ae6 3 Q2HcNvHHml24MFPDHSbbkywgNVg (comment: part of TGS) ------- COMMENT: 5a010480a9612ae6 5 aNxmGsX7+T9t7cBdiXfD7BM2Iao Figure 4B clr3 at telomeres were reduced to the same extent in mutant strains disrupted for either Ccq1 or Taz1, ------- COMMENT: 5a010480a9612ae6 6 aNxmGsX7+T9t7cBdiXfD7BM2Iao Figure 4B clr3 at telomeres were reduced to the same extent in mutant strains disrupted for either Ccq1 or Taz1, ------- COMMENT: 5a010480a9612ae6 7 k1t+SARMThslB9h+e/lIiGw0yis Figure 4B while the levels of Ccq1 at telomeres, relative to those in wild-type cells, were unchanged in clr3D cells but decreased in taz1D ------- COMMENT: 5a010480a9612ae6 8 k1t+SARMThslB9h+e/lIiGw0yis Figure 4B while the levels of Ccq1 at telomeres, relative to those in wild-type cells, were unchanged in clr3D cells but decreased in taz1D ------- COMMENT: 5a010480a9612ae6 9 iuydHNwzuGgYLBauiQuie9lIRAU Figure 4B ------- COMMENT: 5a010480a9612ae6 10 dt0h8RDqeF2L6OyFUpnYYGs4sn0 Figure 4B We found that Clr3 localization at telomere ends was completely abolished in cells defective in both Taz1 and RNAi pathways. ------- COMMENT: 5a010480a9612ae6 11 0T7xExiooZ5+QzpHc+ip2LBkNRU Figure 4B However, defect in RNAi pathway had no impact on Ccq1 localization ------- COMMENT: 5a010480a9612ae6 12 Q2HcNvHHml24MFPDHSbbkywgNVg (comment: part of TGS) ------- COMMENT: 5a010480a9612ae6 33 49AZ+71+u6UVQiW5/ckSbpwB/+A (Figure 4C), Levels of Clr3 and Mit1 were dramati- cally reduced at subtelomeres in swi6 mutant strains ------- COMMENT: 5a010480a9612ae6 34 gkS7XkiQ7oL12HjZx/N2ALLxGfw In contrast to hetero- chromatic loci, SHREC recruitment to euchromatic sites was unaffected in the absence of Swi6, as shown by Clr3 and Mit1 localization at a locus encoding a noncoding RNA and an intergenic region (Figure 4D). ------- COMMENT: 5a010480a9612ae6 35 gkS7XkiQ7oL12HjZx/N2ALLxGfw In contrast to hetero- chromatic loci, SHREC recruitment to euchromatic sites was unaffected in the absence of Swi6, as shown by Clr3 and Mit1 localization at a locus encoding a noncoding RNA and an intergenic region (Figure 4D). ------- COMMENT: 5a010480a9612ae6 36 jPyPabqNCm4o9FNvQqUW/cXRvpg pericentromeric repeats, the silent mat locus, telo- meres, and rDNA loci were derepressed in strains lacking any individual core component of SHREC (Figure 5A). ------- COMMENT: 5a010480a9612ae6 37 jPyPabqNCm4o9FNvQqUW/cXRvpg pericentromeric repeats, the silent mat locus, telo- meres, and rDNA loci were derepressed in strains lacking any individual core component of SHREC (Figure 5A). ------- COMMENT: 5a010480a9612ae6 38 jPyPabqNCm4o9FNvQqUW/cXRvpg pericentromeric repeats, the silent mat locus, telo- meres, and rDNA loci were derepressed in strains lacking any individual core component of SHREC (Figure 5A). ------- COMMENT: 5a010480a9612ae6 39 jPyPabqNCm4o9FNvQqUW/cXRvpg pericentromeric repeats, the silent mat locus, telo- meres, and rDNA loci were derepressed in strains lacking any individual core component of SHREC (Figure 5A). ------- COMMENT: 5a010480a9612ae6 40 jPyPabqNCm4o9FNvQqUW/cXRvpg pericentromeric repeats, the silent mat locus, telo- meres, and rDNA loci were derepressed in strains lacking any individual core component of SHREC (Figure 5A). ------- COMMENT: 5a010480a9612ae6 41 jPyPabqNCm4o9FNvQqUW/cXRvpg pericentromeric repeats, the silent mat locus, telo- meres, and rDNA loci were derepressed in strains lacking any individual core component of SHREC (Figure 5A). ------- COMMENT: 5a010480a9612ae6 42 jPyPabqNCm4o9FNvQqUW/cXRvpg pericentromeric repeats, the silent mat locus, telo- meres, and rDNA loci were derepressed in strains lacking any individual core component of SHREC (Figure 5A). ------- COMMENT: 5a010480a9612ae6 43 jPyPabqNCm4o9FNvQqUW/cXRvpg pericentromeric repeats, the silent mat locus, telo- meres, and rDNA loci were derepressed in strains lacking any individual core component of SHREC (Figure 5A). ------- COMMENT: 5a010480a9612ae6 44 jPyPabqNCm4o9FNvQqUW/cXRvpg pericentromeric repeats, the silent mat locus, telo- meres, and rDNA loci were derepressed in strains lacking any individual core component of SHREC (Figure 5A). ------- COMMENT: 5a010480a9612ae6 45 jPyPabqNCm4o9FNvQqUW/cXRvpg pericentromeric repeats, the silent mat locus, telo- meres, and rDNA loci were derepressed in strains lacking any individual core component of SHREC (Figure 5A). ------- COMMENT: 5a010480a9612ae6 46 jPyPabqNCm4o9FNvQqUW/cXRvpg pericentromeric repeats, the silent mat locus, telo- meres, and rDNA loci were derepressed in strains lacking any individual core component of SHREC (Figure 5A). ------- COMMENT: 5a010480a9612ae6 47 jPyPabqNCm4o9FNvQqUW/cXRvpg pericentromeric repeats, the silent mat locus, telo- meres, and rDNA loci were derepressed in strains lacking any individual core component of SHREC (Figure 5A). ------- COMMENT: 5a010480a9612ae6 48 jPyPabqNCm4o9FNvQqUW/cXRvpg pericentromeric repeats, the silent mat locus, telo- meres, and rDNA loci were derepressed in strains lacking any individual core component of SHREC (Figure 5A). ------- COMMENT: 5a010480a9612ae6 49 jPyPabqNCm4o9FNvQqUW/cXRvpg pericentromeric repeats, the silent mat locus, telo- meres, and rDNA loci were derepressed in strains lacking any individual core component of SHREC (Figure 5A). ------- COMMENT: 5a010480a9612ae6 50 jPyPabqNCm4o9FNvQqUW/cXRvpg pericentromeric repeats, the silent mat locus, telo- meres, and rDNA loci were derepressed in strains lacking any individual core component of SHREC (Figure 5A). ------- COMMENT: 5a010480a9612ae6 51 jPyPabqNCm4o9FNvQqUW/cXRvpg pericentromeric repeats, the silent mat locus, telo- meres, and rDNA loci were derepressed in strains lacking any individual core component of SHREC (Figure 5A). ------- COMMENT: 5a010480a9612ae6 52 jPyPabqNCm4o9FNvQqUW/cXRvpg pericentromeric repeats, the silent mat locus, telo- meres, and rDNA loci were derepressed in strains lacking any individual core component of SHREC (Figure 5A). ------- COMMENT: 5a010480a9612ae6 53 JhfsCkrNUJY3K+pyiBcoyMSlj3k (Figure 5B) we found increases in H3K14ac levels and greater Pol II occupancy at the reporter embedded within pericentromeric heterochromatin in....strains lacking SHREC components ------- COMMENT: 5a010480a9612ae6 54 JhfsCkrNUJY3K+pyiBcoyMSlj3k (Figure 5B) we found increases in H3K14ac levels and greater Pol II occupancy at the reporter embedded within pericentromeric heterochromatin in....strains lacking SHREC components ------- COMMENT: 5a010480a9612ae6 55 JhfsCkrNUJY3K+pyiBcoyMSlj3k (Figure 5B) we found increases in H3K14ac levels and greater Pol II occupancy at the reporter embedded within pericentromeric heterochromatin in....strains lacking SHREC components ------- COMMENT: 5a010480a9612ae6 56 JhfsCkrNUJY3K+pyiBcoyMSlj3k (Figure 5B) we found increases in H3K14ac levels and greater Pol II occupancy at the reporter embedded within pericentromeric heterochromatin in....strains lacking SHREC components ------- COMMENT: 5a010480a9612ae6 57 JhfsCkrNUJY3K+pyiBcoyMSlj3k (Figure 5B) we found increases in H3K14ac levels and greater Pol II occupancy at the reporter embedded within pericentromeric heterochromatin in....strains lacking SHREC components ------- COMMENT: 5a010480a9612ae6 58 5f3Hev1DaR9ArTzW2TBtFsWJwmE The role of SHREC in transcriptional silencing could be decoupled from the cis-PTGS function of the RNAi machinery since impaired SHREC had no effect on the localization of RITS Agol subunit at heterochromatin (Figure 5D). ------- COMMENT: 5a010480a9612ae6 59 bwX2tLDWYKAtww6U/XMrBf6fLaI (Figure 5E). Enhanced transcriptional-machinery occupancy at heterochromatic repeats in SHREC defective cells,..should result in elevated repeat transcripts ...corresponding increase in siRNA production. ------- COMMENT: 5a010480a9612ae6 60 bwX2tLDWYKAtww6U/XMrBf6fLaI (Figure 5E). Enhanced transcriptional-machinery occupancy at heterochromatic repeats in SHREC defective cells,..should result in elevated repeat transcripts ...corresponding increase in siRNA production. ------- COMMENT: 5a010480a9612ae6 61 bwX2tLDWYKAtww6U/XMrBf6fLaI (Figure 5E). Enhanced transcriptional-machinery occupancy at heterochromatic repeats in SHREC defective cells,..should result in elevated repeat transcripts ...corresponding increase in siRNA production. ------- COMMENT: 5a010480a9612ae6 62 bwX2tLDWYKAtww6U/XMrBf6fLaI (Figure 5E). Enhanced transcriptional-machinery occupancy at heterochromatic repeats in SHREC defective cells,..should result in elevated repeat transcripts ...corresponding increase in siRNA production. ------- COMMENT: 5a010480a9612ae6 63 Epi9T3MJJkk7ywnfB0/CVjtSxXY (Figure 6A) both clr3D232N and mit1K587A mutant alleles alleviated silencing of a marker gene inserted at pericentromeric repeats ------- COMMENT: 5a010480a9612ae6 64 Epi9T3MJJkk7ywnfB0/CVjtSxXY (Figure 6A) both clr3D232N and mit1K587A mutant alleles alleviated silencing of a marker gene inserted at pericentromeric repeats ------- COMMENT: 5a010480a9612ae6 65 wqqzVqoLeYl1e8T/yPwhsoyq0aU Figure 6B ------- COMMENT: 5a010480a9612ae6 66 7584mSlyG4GCDEMox2J5rU6B6A8 (Figure 6B) ------- COMMENT: 5a010480a9612ae6 67 wqqzVqoLeYl1e8T/yPwhsoyq0aU Figure 6B ------- COMMENT: 5a010480a9612ae6 68 wqqzVqoLeYl1e8T/yPwhsoyq0aU Figure 6B ------- COMMENT: 5a010480a9612ae6 69 LRks/wD862o74PJaNd8T37ISYT8 (comment: by TGS) ------- COMMENT: 5a010480a9612ae6 73 mqBF3ZldU2bITI3pFuP0XlDx/WA Figure 6D ------- COMMENT: 5a010480a9612ae6 74 mqBF3ZldU2bITI3pFuP0XlDx/WA Figure 6D ------- COMMENT: 5a010480a9612ae6 77 nEaa8YKAJQrgM8JMf+0co+MNKbo Figure 6E ------- COMMENT: 5a010480a9612ae6 78 LRks/wD862o74PJaNd8T37ISYT8 (comment: by TGS) ------- COMMENT: 5a010480a9612ae6 79 LRks/wD862o74PJaNd8T37ISYT8 (comment: by TGS) ------- COMMENT: 5a010480a9612ae6 80 LRks/wD862o74PJaNd8T37ISYT8 (comment: by TGS) ------- COMMENT: 5a010480a9612ae6 81 LRks/wD862o74PJaNd8T37ISYT8 (comment: by TGS) ------- COMMENT: 5a010480a9612ae6 82 LRks/wD862o74PJaNd8T37ISYT8 (comment: by TGS) ------- COMMENT: 5a010480a9612ae6 83 LRks/wD862o74PJaNd8T37ISYT8 (comment: by TGS) ------- COMMENT: 5a010480a9612ae6 84 LRks/wD862o74PJaNd8T37ISYT8 (comment: by TGS) ------- COMMENT: 5a010480a9612ae6 85 LRks/wD862o74PJaNd8T37ISYT8 (comment: by TGS) ------- COMMENT: 5a010480a9612ae6 86 LRks/wD862o74PJaNd8T37ISYT8 (comment: by TGS) ------- COMMENT: 5a010480a9612ae6 87 LRks/wD862o74PJaNd8T37ISYT8 (comment: by TGS) ------- COMMENT: 5a010480a9612ae6 88 LRks/wD862o74PJaNd8T37ISYT8 (comment: by TGS) ------- COMMENT: 5a010480a9612ae6 89 LRks/wD862o74PJaNd8T37ISYT8 (comment: by TGS) ------- COMMENT: 5a010480a9612ae6 90 LRks/wD862o74PJaNd8T37ISYT8 (comment: by TGS) ------- COMMENT: 5a010480a9612ae6 91 LRks/wD862o74PJaNd8T37ISYT8 (comment: by TGS) ------- COMMENT: 5a010480a9612ae6 92 LRks/wD862o74PJaNd8T37ISYT8 (comment: by TGS) ------- COMMENT: 5a546d70bc11f7b5 1 TvP08QpqTkJq29tH/hKb8vOB/LM Localization of Epe1-GFP re- vealed two to three discrete foci at the nuclear periphery (Figure S1 available in the Supplemental Data with this article online), in a pattern that is similar to those of proteins associated with heterochromatin such as Swi6 (Ekwall et al., 1995). ------- COMMENT: 5a546d70bc11f7b5 2 klahwpsx4hgwF5sH5/2Vu+j7cik Moreover, Epe1 was de- tected at certain meiotic genes, some of which are coated with heterochromatic markers (Figure 2A; [Cam et al., 2005]). ------- COMMENT: 5a546d70bc11f7b5 3 ZUS+LIa5iWFOV0GbHuAqfcOM1PA heterochromatic loci, including centromeres, telomeres, and the mat locus (Figure 2A). ------- COMMENT: 5a546d70bc11f7b5 4 ZUS+LIa5iWFOV0GbHuAqfcOM1PA heterochromatic loci, including centromeres, telomeres, and the mat locus (Figure 2A). ------- COMMENT: 5a546d70bc11f7b5 5 ZUS+LIa5iWFOV0GbHuAqfcOM1PA heterochromatic loci, including centromeres, telomeres, and the mat locus (Figure 2A). ------- COMMENT: 5a546d70bc11f7b5 8 klahwpsx4hgwF5sH5/2Vu+j7cik Moreover, Epe1 was de- tected at certain meiotic genes, some of which are coated with heterochromatic markers (Figure 2A; [Cam et al., 2005]). ------- COMMENT: 5a546d70bc11f7b5 9 klahwpsx4hgwF5sH5/2Vu+j7cik Moreover, Epe1 was de- tected at certain meiotic genes, some of which are coated with heterochromatic markers (Figure 2A; [Cam et al., 2005]). ------- COMMENT: 5a546d70bc11f7b5 10 klahwpsx4hgwF5sH5/2Vu+j7cik Moreover, Epe1 was de- tected at certain meiotic genes, some of which are coated with heterochromatic markers (Figure 2A; [Cam et al., 2005]). ------- COMMENT: 5a546d70bc11f7b5 11 klahwpsx4hgwF5sH5/2Vu+j7cik Moreover, Epe1 was de- tected at certain meiotic genes, some of which are coated with heterochromatic markers (Figure 2A; [Cam et al., 2005]). ------- COMMENT: 5a546d70bc11f7b5 12 klahwpsx4hgwF5sH5/2Vu+j7cik Moreover, Epe1 was de- tected at certain meiotic genes, some of which are coated with heterochromatic markers (Figure 2A; [Cam et al., 2005]). ------- COMMENT: 5a546d70bc11f7b5 13 klahwpsx4hgwF5sH5/2Vu+j7cik Moreover, Epe1 was de- tected at certain meiotic genes, some of which are coated with heterochromatic markers (Figure 2A; [Cam et al., 2005]). ------- COMMENT: 5a546d70bc11f7b5 14 gQqC9G+hbmHVkX4QMJAktJSvMu4 In swi6 mutant background, Epe1-GFP was no longer localized to discrete spots at the nuclear periphery (Figure 3A). ------- COMMENT: 5a546d70bc11f7b5 15 T9kVGOYgKtmJXStlxwWSmZoDdiQ Moreover, ChIP analy- sis revealed that mutation in Swi6 or an H3K9-specific methyltransferase Clr4 abolished Epe1 localization at centromeres, telomeres, and the mat locus, concurrent with loss of Swi6 at these loci (Figure 3B) ------- COMMENT: 5a546d70bc11f7b5 16 T9kVGOYgKtmJXStlxwWSmZoDdiQ Moreover, ChIP analy- sis revealed that mutation in Swi6 or an H3K9-specific methyltransferase Clr4 abolished Epe1 localization at centromeres, telomeres, and the mat locus, concurrent with loss of Swi6 at these loci (Figure 3B) ------- COMMENT: 5a546d70bc11f7b5 17 T9kVGOYgKtmJXStlxwWSmZoDdiQ Moreover, ChIP analy- sis revealed that mutation in Swi6 or an H3K9-specific methyltransferase Clr4 abolished Epe1 localization at centromeres, telomeres, and the mat locus, concurrent with loss of Swi6 at these loci (Figure 3B) ------- COMMENT: 5a546d70bc11f7b5 18 nxpEH67saOwUyOcUcoTZXvXbY9A a glutathione S-transferase (GST) pull-down assay showed that in vitro-translated Epe1 directly binds to GST-Swi6, but not GST alone (Figure 3D). This inter- action is not affected by a mutation in JmjC domain of Epe1 (Y307A) that impairs Epe1 function. Based on these analyses, Swi6-mediated recruitment of Epe1 might involve direct interaction between these factors. ChIP analysis revealed that muta- tions in swi6 or clr4 resulted in loss of Epe1 from mcp5, mcp7, mei4, and ssm4, and from the LC region (Figures 4A and 4B), suggesting Swi6 facilitated recruit- ment of Epe1 to these loci. ------- COMMENT: 5a546d70bc11f7b5 19 ZeSHaw76ssmcGmj1DnjwikH7Jgw However, abundant transcripts corresponding to cen- tromeric repeats and cenH are detectable in RNAi- defective Dago1 cells. We found that the levels of tran- scripts in Depe1Dago1 double mutant cells were significantly reduced (Figures 5A and 5B), indicating that Epe1 facilitates transcription of the heterochromatic repeats. ------- COMMENT: 5a546d70bc11f7b5 20 ZeSHaw76ssmcGmj1DnjwikH7Jgw However, abundant transcripts corresponding to cen- tromeric repeats and cenH are detectable in RNAi- defective Dago1 cells. We found that the levels of tran- scripts in Depe1Dago1 double mutant cells were significantly reduced (Figures 5A and 5B), indicating that Epe1 facilitates transcription of the heterochromatic repeats. ------- COMMENT: 5a546d70bc11f7b5 21 dKCYdlRJrTvS3Sn5/ohFJGuATQk Our analysis revealed that Epe1 is required for the increase in transcription of repeats in Dclr3 back- ground, as suggested by the dramatic reduction in tran- script levels in Depe1 Dclr3 double mutant compared to Dclr3 single mutant (Figures 5A and 5B). ------- COMMENT: 5a546d70bc11f7b5 22 dKCYdlRJrTvS3Sn5/ohFJGuATQk Our analysis revealed that Epe1 is required for the increase in transcription of repeats in Dclr3 back- ground, as suggested by the dramatic reduction in tran- script levels in Depe1 Dclr3 double mutant compared to Dclr3 single mutant (Figures 5A and 5B). ------- COMMENT: 5a546d70bc11f7b5 23 c6g2A2huNVJI++L0NHBQo9HxYt4 We found that loss of Epe1 restores Swi6 localization and silencing in Dago1 and Dclr3 mutants to levels comparable to wild-type cells (Figure 5C and Figure S3), ------- COMMENT: 5a546d70bc11f7b5 24 c6g2A2huNVJI++L0NHBQo9HxYt4 We found that loss of Epe1 restores Swi6 localization and silencing in Dago1 and Dclr3 mutants to levels comparable to wild-type cells (Figure 5C and Figure S3), ------- COMMENT: 5a546d70bc11f7b5 25 lpnvHWQ6fxPw8wjZBMV7weMchmw Indeed, in a situation wherein heterochromatin has been completely abol- ished, such as in a Dclr4 background, loss of Epe1 had no detectable effect on transcript levels (Figure 5D). ------- COMMENT: 5a546d70bc11f7b5 26 lpnvHWQ6fxPw8wjZBMV7weMchmw Indeed, in a situation wherein heterochromatin has been completely abol- ished, such as in a Dclr4 background, loss of Epe1 had no detectable effect on transcript levels (Figure 5D). ------- COMMENT: 5a546d70bc11f7b5 27 EdCzTeeFlMXkP+oqE7SwM0v2da4 Deletion of clr3 re- sults in a dramatic increase in Pol II occupancy at cenH element within silent mat domain. ------- COMMENT: 5a546d70bc11f7b5 28 hbNlMs0if/DHxkqQsGROeheARRY Remarkably, loss of Epe1 resulted in a diminished access of Pol II to cenH in Dclr3 cells, as indicated by substantial reduction in Pol II levels in Depe1 Dclr3 double mutant as compared to Dclr3 single mutant (Figure 5E). ------- COMMENT: 5a546d70bc11f7b5 29 a7ieMn1PVFNWgtpvzWcao80scxE Remarkably, loss of Epe1 resulted in a diminished access of Pol II to cenH in Dclr3 cells, as indicated by substantial reduction in Pol II levels in Depe1 Dclr3 double mutant as compared to Dclr3 single mutant (Figure 5E). These results suggest that Epe1 has an important role in promoting access of transcriptional machinery to heterochromatic sequences, thereby facilitating transcription of repeat elements. Therefore, the balance of activities between Clr3 and Epe1, both of which are recruited by Swi6, seems critical in determining the transcriptional state of repeat ele- ments. ------- COMMENT: 5a546d70bc11f7b5 30 4lR1/0Qjw2rZ4xzy3NyRNja41W0 Indeed, we found that loss of H3K9me2 in cells overexpressing Epe1 is de- pendent upon Swi6 (Figure 6D). ------- COMMENT: 5a546d70bc11f7b5 31 yqkPNv5QT2OtwEI+M8VhjlRYqOo We also tested the effect of Epe1 on heterochromatic markers at meiotic genes. Interestingly, loss of Epe1 re- sulted in considerable increase in H3K9me2 and H3K9me3 levels, concomitant with moderate increase in Swi6 binding at the ssm4 gene (Figures 6E and 6F). ------- COMMENT: 5a546d70bc11f7b5 33 6nMNpZWiB890sknKezXmmc9NPA8 A similar increase in H3K9me2 levels was also observed in swi6 mutant cells defective in Epe1 recruitment to meiotic genes (Figure 6E). ------- COMMENT: 5a546d70bc11f7b5 35 SwFpE8UVNYAYP05eL/hH5Blho9c The Y307 mutation abolished the ability of Epe1 to destabilize heterochromatic silencing (Figure 6A). ------- COMMENT: 5a546d70bc11f7b5 36 8vnSMYKAA71CA/pWS8ihzNUd1mU ChIP analysis showed that mutant protein is recruited to heterochromatic loci (Figure 6B), consis- tent with data showing that Y307A mutation has no ef- fect on Epe1 interaction with Swi6 in vitro (Figure 3D) ------- COMMENT: 5a546d70bc11f7b5 37 L2mU74SxdjUI8ZQvCbIx0dWpKmc We next investigated the possible involvement of Epe1 in boundary function of the IRC elements. Remarkably, deletion of epe1 resulted in spreading of Swi6 and H3K9me into euchromatic regions surrounding cen1 (Figure 7D). ------- COMMENT: 5a6fef3458353ae1 79 omAPup9JOWXrUG1C9gMgww+c9Xk This finding suggested that inactive AMPK is excluded from the nucleus and, upon activation by glucose or nitrogen starvation, part of the AMPK moves into the nucleus. ------- COMMENT: 5a6fef3458353ae1 80 PeBy6nWsC46itQCGWOS5PsRhw4w These results indicated that Ssp2 interacts physically with Amk2 and Cbs2 in vivo, showing that AMPK is indeed a αβγ heterotrimer and that these three subunits interact even under optimal growth conditions. ------- COMMENT: 5a6fef3458353ae1 81 PeBy6nWsC46itQCGWOS5PsRhw4w These results indicated that Ssp2 interacts physically with Amk2 and Cbs2 in vivo, showing that AMPK is indeed a αβγ heterotrimer and that these three subunits interact even under optimal growth conditions. ------- COMMENT: 5a6fef3458353ae1 82 PeBy6nWsC46itQCGWOS5PsRhw4w These results indicated that Ssp2 interacts physically with Amk2 and Cbs2 in vivo, showing that AMPK is indeed a αβγ heterotrimer and that these three subunits interact even under optimal growth conditions. ------- COMMENT: 5a6fef3458353ae1 83 KMS9/7wlWUfMjrfZLvW1wxQ6u68 AMPK is required for proper advance entry into mitosis in nitrogen-starved cells and arrest in G1 before Start. ------- COMMENT: 5a6fef3458353ae1 84 xMbFclMBydLff560UHoEzvL5sMc ------- COMMENT: 5a6fef3458353ae1 85 xMbFclMBydLff560UHoEzvL5sMc ------- COMMENT: 5a6fef3458353ae1 87 sAsJBwsSBh5bNhOZHwBDbKzjmMI This result shows that Ssp2 phosphorylation by Ssp1 is required to trigger the nuclear accumulation of the former upon nitrogen or glucose starvation, and that if Ssp2 is not phosphorylated it remains in a cytoplasmic localization, regardless of the nutritional conditions of the cell. ------- COMMENT: 5a6fef3458353ae1 89 KMS9/7wlWUfMjrfZLvW1wxQ6u68 AMPK is required for proper advance entry into mitosis in nitrogen-starved cells and arrest in G1 before Start. ------- COMMENT: 5a6fef3458353ae1 90 KMS9/7wlWUfMjrfZLvW1wxQ6u68 AMPK is required for proper advance entry into mitosis in nitrogen-starved cells and arrest in G1 before Start. ------- COMMENT: 5a6fef3458353ae1 91 KMS9/7wlWUfMjrfZLvW1wxQ6u68 AMPK is required for proper advance entry into mitosis in nitrogen-starved cells and arrest in G1 before Start. ------- COMMENT: 5a8021665769078b 4 VSxvX45cZrLNmDvASfYNizs3MGY fig 1 brc1 mutant cells expressing brc1-T672A are deficient in Nse4 foci formation ------- COMMENT: 5a8021665769078b 5 g1Eo3CKjP76gTrOC6hCoGeRl0RI fig 1 a: brc1 mutant abolishes Nse4 nuclear foci in HU/MMS treated cells ------- COMMENT: 5a8021665769078b 6 lW14NucXTy23snO8CHU2j6cUBc0 ------- COMMENT: 5a8021665769078b 7 sf+jIN+eFrfD5e8Z8jXGcP8mw90 ------- COMMENT: 5a8021665769078b 8 BCO/k3ZoY9wiH2Gae39p18nN2JI ------- COMMENT: 5a8021665769078b 9 HMxJKLwOctQ+E4LRNBYva1rUFV4 deletion of Nse6 strongly reduced Nse4 residence at binding sites tested under normal and genotoxic stress ------- COMMENT: 5a8021665769078b 10 VGmJdJiKwc3FOa2FtEXb66rODzg deletion of Nse5 strongly reduced Nse4 residence at binding sites tested under normal and genotoxic stress ------- COMMENT: 5a8021665769078b 11 4qoMnhGJyqD9c/mGeeRQ/mlNeC8 deletion of Brc1 significantly reduced Nse4 residence at binding sites tested under normal and genotoxic stress ------- COMMENT: 5a8021665769078b 12 mITDifczw4vw/xLxQsXtfuJ0Bd4 Nse4 sumoylation undetectable in nse6Δ ------- COMMENT: 5a8021665769078b 13 k66toLK6mR3DhfJy2PXlE95wWJo Nse4 sumoylation reduced in brc1Δ ------- COMMENT: 5a8021665769078b 14 pTRGMT+dei/cX6+0wRolV0GpwRM Nse4 sumoylation at wild type level ------- COMMENT: 5a8021665769078b 16 BI2NoYnUlfIp3c7gN4EsNIUhFdA mutations in brc1 weaken interaction with nse6 ------- COMMENT: 5a8021665769078b 17 rGVHKQJoMQHJftOXhffgvUj44IM nse2-SA brc1􏰀 cells are more sensitive to genotoxins than either single mutant (Fig. 4C). ------- COMMENT: 5a8021665769078b 21 NeKK2+t2I42cRMvbioQiQbhM8/0 Notably, however, deletion of either Nse5 or Nse6 strongly reduced Nse4 residence at all binding sites tested, which was most evident under conditions of genotoxic stress that stimu- late de novo Smc5-Smc6 loading ------- COMMENT: 5a8021665769078b 22 NeKK2+t2I42cRMvbioQiQbhM8/0 Notably, however, deletion of either Nse5 or Nse6 strongly reduced Nse4 residence at all binding sites tested, which was most evident under conditions of genotoxic stress that stimu- late de novo Smc5-Smc6 loading ------- COMMENT: 5a8021665769078b 24 KxikFh6+KkFisfGMvrAWegJnKbk As anticipated, the MMS-induced SUMOylation of Nse4-TAP in wild-type cells was detectable upon immunoprecipitation (IP) of Nse4 without overexpression or initial enrichment of SUMO (Fig. 4A) ------- COMMENT: 5a8021665769078b 26 xs7CWcWtbb2vmNUu0eJ8J6z7f/g MMS-induced Nse4 SUMOylation was also reduced in brc1􏰀 cells but was similar to wild-type in rhp18􏰀 cells, which support normal Nse4-GFP focus formation (Fig. 4B) ------- COMMENT: 5a8021665769078b 27 BCO/k3ZoY9wiH2Gae39p18nN2JI Nse4 foci gone in nse6 mutant cells ------- COMMENT: 5aa10f6490885b2e 1 McJwJcQznbtacAV5T13EEpIlt/g Dip1 activates Arp2/3 complex to nucleate linear actin filaments analogous to branched actin filaments created by Wsp1-mediated Arp2/3 complex activation. These Dip1-Arp2/3 complex nucleated filaments act as seeds for Wsp1-mediated Arp2/3 complex branching nucleation. ------- COMMENT: 5b029ec9b56c8283 1 bO2UUwxanoeqDiIMasiXuraI28U During G2, the concentration of Cdc25 increases about 2 fold (Figure 1A) ------- COMMENT: 5b029ec9b56c8283 5 aeWuHa2bMDH8SybQExksxFELLhs ------- COMMENT: 5b029ec9b56c8283 6 aeWuHa2bMDH8SybQExksxFELLhs ------- COMMENT: 5b029ec9b56c8283 7 ScK8h/nfeL9Sfojy1LvknAuuL54 relatively constant concentration during G2, as previously observed ------- COMMENT: 5b029ec9b56c8283 9 eTfpgHbi04WB70rD9Pp4NHbnbQg We find that the size dependent expression of Cdc25 does not require the 5′ UTR of its transcript, showing that this mechanism of translational regulation is not necessary for size dependent expression (Figure S3A). ------- COMMENT: 5b029ec9b56c8283 10 O8kxZSdNkKAiWHvLe6iToVFuawg (comment: CHECK normal cell size homeostasis) ------- COMMENT: 5b029ec9b56c8283 14 hEQwSTwZFpNcJhEsp39Za2+T5UA We propose that the size-dependent expression of the cdc25 transcript is the mechanism that allows cells to divide at a particular size, but it is not the mechanism which regulates what that size is. ------- COMMENT: 5b1cec4b5c194281 1 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 5b1cec4b5c194281 2 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 5b1cec4b5c194281 3 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 5b1cec4b5c194281 4 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 5b1cec4b5c194281 5 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 5b1cec4b5c194281 6 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 5b1cec4b5c194281 7 VBSG41xZYrzV3i7jL0SktT8Vq2s fig3 B-2 (comment: 1.1% WT) ------- COMMENT: 5b1cec4b5c194281 8 vVQCl1lumL1CdrluaoV7rXXscj0 fig3 B-2 (comment: never seen in WT) ------- COMMENT: 5b1cec4b5c194281 9 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: 5b1cec4b5c194281 12 C4JbvDp/bHUCJq1AbM3MhuIS7+4 (comment: DNA at the tips, telophase delay) ------- COMMENT: 5b1cec4b5c194281 16 hHu3B09nVzzmEA89uE90ENexhDs Figure 3F-3 ------- COMMENT: 5b1cec4b5c194281 17 hHu3B09nVzzmEA89uE90ENexhDs Figure 3F-3 ------- COMMENT: 5b1cec4b5c194281 18 hHu3B09nVzzmEA89uE90ENexhDs Figure 3F-3 ------- COMMENT: 5b1cec4b5c194281 19 hHu3B09nVzzmEA89uE90ENexhDs Figure 3F-3 ------- COMMENT: 5b1cec4b5c194281 21 WI5tAkcerjyhlsXweoj8DX9nnxU Figure 4D ------- COMMENT: 5b1cec4b5c194281 22 WI5tAkcerjyhlsXweoj8DX9nnxU Figure 4D ------- COMMENT: 5b1cec4b5c194281 23 WI5tAkcerjyhlsXweoj8DX9nnxU Figure 4D ------- COMMENT: 5b1cec4b5c194281 24 WI5tAkcerjyhlsXweoj8DX9nnxU Figure 4D ------- COMMENT: 5b1cec4b5c194281 27 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw Figure 2 ------- COMMENT: 5b1cec4b5c194281 28 5Pc+GJjJVhHm1h5Cm22Rhx2qUks Figure 5B ------- COMMENT: 5b1cec4b5c194281 29 5Pc+GJjJVhHm1h5Cm22Rhx2qUks Figure 5B ------- COMMENT: 5b59bb193448a673 7 91QD0iRRDPHxekvEZyyKDHDKDn0 (comment: covalent binding between topoisomerase and DNA) ------- COMMENT: 5b59bb193448a673 29 91QD0iRRDPHxekvEZyyKDHDKDn0 (comment: covalent binding between topoisomerase and DNA) ------- COMMENT: 5b59bb193448a673 30 91QD0iRRDPHxekvEZyyKDHDKDn0 (comment: covalent binding between topoisomerase and DNA) ------- COMMENT: 5b59bb193448a673 31 91QD0iRRDPHxekvEZyyKDHDKDn0 (comment: covalent binding between topoisomerase and DNA) ------- COMMENT: 5b59bb193448a673 35 91QD0iRRDPHxekvEZyyKDHDKDn0 (comment: covalent binding between topoisomerase and DNA) ------- COMMENT: 5b59bb193448a673 38 91QD0iRRDPHxekvEZyyKDHDKDn0 (comment: covalent binding between topoisomerase and DNA) ------- COMMENT: 5b59bb193448a673 39 91QD0iRRDPHxekvEZyyKDHDKDn0 (comment: covalent binding between topoisomerase and DNA) ------- COMMENT: 5b68d89f4f39e301 1 asl5CLeNjI9G8VkrqlSnrldZLV4 Fig S1A, Fig1 ------- COMMENT: 5b68d89f4f39e301 3 AXEuaylOvMdIZA2aZazQ3Z/ynvs Fig S1A, Fig1B,C ------- COMMENT: 5b68d89f4f39e301 4 bRjELTffvsNDt4ZWwvNCdI+F5DM Fig 2, Fig3, Fig4B ------- COMMENT: 5b68d89f4f39e301 5 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 5b68d89f4f39e301 6 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 5b68d89f4f39e301 7 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 5b68d89f4f39e301 8 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 5b68d89f4f39e301 9 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 5b68d89f4f39e301 10 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 5b68d89f4f39e301 11 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 5b68d89f4f39e301 12 As6y+kpzP9SCbt87noFKAVISyn8 Fig4A, C, E, F (comment: no supplements added) ------- COMMENT: 5b68d89f4f39e301 13 oSThggxIX8+hRSjzlK1gIaHmjyY Fig4D (comment: no supplements added) ------- COMMENT: 5b68d89f4f39e301 14 ptzKTtSTAaLdtKyTLcGHzVc3Snw Fig S4A cell growth at G2/M1 arrest is dependent on transcription. (comment: no supplements added) ------- COMMENT: 5b68d89f4f39e301 15 ntB1ZW+oTd5Cv5sIDtziXRWmjp4 FigS5 in this situation Sck1 only causes a small increase in cell length increase compared to Sck2 . This show that Sck2 is the main S6 kinase effector of promoting growth in this situation ------- COMMENT: 5b68d89f4f39e301 16 foOyg2JUlxsbIGqYkE0Rqq1sA/c FigS5 in this situation psk1 only causes a small increase in cell length increase compared to Sck2 . This show that Sck2 is the main S6 kinase effector of promoting growth in this situation ------- COMMENT: 5b68d89f4f39e301 17 BPHAEq+lNgaE7V9whGIEGONl8IM FigS5 in this situation gad8 overexpression is unable to promote growth i. This show that Sck2 is the main S6 kinase effector of promoting growth in this situation ------- COMMENT: 5b68d89f4f39e301 18 /7sA+CZO40H4AQ3C44BEigOrpjE Fig5 (comment: supplements added) ------- COMMENT: 5b68d89f4f39e301 19 o4qBG0sxYOWC+7hjxsZgOTgQqT4 Fig 5 (comment: supplements added) ------- COMMENT: 5b68d89f4f39e301 20 o4qBG0sxYOWC+7hjxsZgOTgQqT4 Fig 5 (comment: supplements added) ------- COMMENT: 5b68d89f4f39e301 21 o4qBG0sxYOWC+7hjxsZgOTgQqT4 Fig 5 (comment: supplements added) ------- COMMENT: 5b68d89f4f39e301 22 o4qBG0sxYOWC+7hjxsZgOTgQqT4 Fig 5 (comment: supplements added) ------- COMMENT: 5b68d89f4f39e301 23 o4qBG0sxYOWC+7hjxsZgOTgQqT4 Fig 5 (comment: supplements added) ------- COMMENT: 5b68d89f4f39e301 24 o4qBG0sxYOWC+7hjxsZgOTgQqT4 Fig 5 (comment: supplements added) ------- COMMENT: 5b68d89f4f39e301 25 o4qBG0sxYOWC+7hjxsZgOTgQqT4 Fig 5 (comment: supplements added) ------- COMMENT: 5b68d89f4f39e301 26 o4qBG0sxYOWC+7hjxsZgOTgQqT4 Fig 5 (comment: supplements added) ------- COMMENT: 5b68d89f4f39e301 27 o4qBG0sxYOWC+7hjxsZgOTgQqT4 Fig 5 (comment: supplements added) ------- COMMENT: 5b68d89f4f39e301 28 o4qBG0sxYOWC+7hjxsZgOTgQqT4 Fig 5 (comment: supplements added) ------- COMMENT: 5b68d89f4f39e301 29 o4qBG0sxYOWC+7hjxsZgOTgQqT4 Fig 5 (comment: supplements added) ------- COMMENT: 5b68d89f4f39e301 30 o4qBG0sxYOWC+7hjxsZgOTgQqT4 Fig 5 (comment: supplements added) ------- COMMENT: 5b7414d8bc7f80eb 1 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: 5b7414d8bc7f80eb 2 ekDPQHbAnbe6qq1nVLc0Qsg/Ow0 Fig 1A ------- COMMENT: 5b7414d8bc7f80eb 3 gNT/IjPH/j+8Q0aT3+W5ORpKW7k Fig 1B (comment: cell cycle arrest with post anaphase microtubule array) ------- COMMENT: 5b7414d8bc7f80eb 4 lxYA9M46TKfHyJe4/ybzCuF6Is4 Fig 1B, C Cells arrest with a stable actinomyosin ring and fail to undergo cytokinesis ------- COMMENT: 5b7414d8bc7f80eb 6 Dwxh4bkXY/PZQq8pPpngexTwIIA Fig2 E,F ------- COMMENT: 5b7414d8bc7f80eb 7 YGTHdFPWAmmR96d6Ldv0NPUQzEU Fig 3A ------- COMMENT: 5b7414d8bc7f80eb 8 t6uyTtt4P/8kRlIjC2qelSNbzfs Fig 3C ------- COMMENT: 5b7414d8bc7f80eb 9 t6uyTtt4P/8kRlIjC2qelSNbzfs Fig 3C ------- COMMENT: 5b7414d8bc7f80eb 10 t6uyTtt4P/8kRlIjC2qelSNbzfs Fig 3C ------- COMMENT: 5b7414d8bc7f80eb 11 xd4KvnbsTU3VEvzQ7GEYqaezy6s Fig 3E,F ------- COMMENT: 5b7414d8bc7f80eb 12 t6uyTtt4P/8kRlIjC2qelSNbzfs Fig 3C ------- COMMENT: 5b7414d8bc7f80eb 13 0KdROd53Xfle5NBuMNMP6Vo5dHk Fig 3E ------- COMMENT: 5b7414d8bc7f80eb 14 0KdROd53Xfle5NBuMNMP6Vo5dHk Fig 3E ------- COMMENT: 5b7414d8bc7f80eb 15 Dwxh4bkXY/PZQq8pPpngexTwIIA Fig2 E,F ------- COMMENT: 5b7414d8bc7f80eb 17 gC4BOe02CE9sBIY34q8pAugtGaA Fig 4C (displacemetn is supressed by inhibiting membrane trafficking ------- COMMENT: 5b7414d8bc7f80eb 18 urOpmJ7V2cOTeJDX6yi2ZCNyVFA Fig 4D ------- COMMENT: 5b7414d8bc7f80eb 20 YsAmF4EP/sce5Z/3NgAEpF+CkuU Fig 4D, E, F ------- COMMENT: 5b7414d8bc7f80eb 21 YsAmF4EP/sce5Z/3NgAEpF+CkuU Fig 4D, E, F ------- COMMENT: 5b7414d8bc7f80eb 22 HJxOo9dlzCUeT14LjuCLlLTKqqw data not shown ------- COMMENT: 5b7414d8bc7f80eb 23 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 5b7414d8bc7f80eb 24 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 5b7414d8bc7f80eb 25 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 5bba706f5b371433 141 tI4Lgi90H7NTjQKyVi4JPufzfzE (comment: silencing at otr) ------- COMMENT: 5bba706f5b371433 142 tI4Lgi90H7NTjQKyVi4JPufzfzE (comment: silencing at otr) ------- COMMENT: 5bba706f5b371433 143 tI4Lgi90H7NTjQKyVi4JPufzfzE (comment: silencing at otr) ------- COMMENT: 5bba706f5b371433 144 tI4Lgi90H7NTjQKyVi4JPufzfzE (comment: silencing at otr) ------- COMMENT: 5bba706f5b371433 145 tI4Lgi90H7NTjQKyVi4JPufzfzE (comment: silencing at otr) ------- COMMENT: 5bba706f5b371433 146 tI4Lgi90H7NTjQKyVi4JPufzfzE (comment: silencing at otr) ------- COMMENT: 5bba706f5b371433 147 tI4Lgi90H7NTjQKyVi4JPufzfzE (comment: silencing at otr) ------- COMMENT: 5bba706f5b371433 148 tI4Lgi90H7NTjQKyVi4JPufzfzE (comment: silencing at otr) ------- COMMENT: 5bba706f5b371433 159 ZgdrmiEV/DVPAv2vP+vI+dE0DAI (comment: at pericentromeric regions) ------- COMMENT: 5bd37a86db70f418 1 iyINNJNJ0ieECrnj4LdQ9H7rSKo fig 1AB ------- COMMENT: 5bd37a86db70f418 2 iyINNJNJ0ieECrnj4LdQ9H7rSKo fig 1AB ------- COMMENT: 5bd37a86db70f418 3 dSPXz3a7KD/Rk6ZwiMb88ZZB2Eg fig 1C. figure 2 ------- COMMENT: 5bd37a86db70f418 4 gm1Px94b5UBdjxqoHSq63NZdxOQ table 1 ------- COMMENT: 5bd37a86db70f418 5 gm1Px94b5UBdjxqoHSq63NZdxOQ table 1 ------- COMMENT: 5bd37a86db70f418 6 gm1Px94b5UBdjxqoHSq63NZdxOQ table 1 ------- COMMENT: 5bd37a86db70f418 7 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: 5bd37a86db70f418 8 nnuWhrFU7jaQ2CsXT4A21I2oLbM fig 3A ------- COMMENT: 5bd37a86db70f418 11 LZr8VHAd+GNgnXMYV0DRzfGzrZE fig 4 abnormally segregating nuclear membrane #2863 PENDING ------- COMMENT: 5beca4251520f38b 40 hFJDx36MpObgXoRdYMWoYuia3Yg important when growing on poor nitrogen sources ------- COMMENT: 5beca4251520f38b 62 Iifh+XC5psEkoW2uKngre8iWCQc (comment: vw: changed to directly activates and added part_of They are deomstrating that the system is conserved...In Xenopus and mammalian cells, phosphorylation of ENSA by greatwall at serine 67 promotes its binding to and inhibition of PP2A$B55 phosphatase [8, 9]. enough evidence for function by IMP ALSO Our genetic and physiological data is in agreement with published work in budding yeast, Drosophila, Xenopus, and mammalian cells indicating that greatwall phosphorylates endo- sulfine to inhibit PP2A$B55 [8, 9, 27, 28, 35]. To test whether Igo1 is a direct target of Ppk18, we performed Ppk18 in vitro kinase assays using purified recombinant Igo1 and Igo1-S64A, as sub- strates. Extracts from wild-type (ppk18+) and Myc-tagged (ppk18-13myc) Ppk18 cells, treated for 1 hr with rapamycin in or- der to activate Ppk18, were immunoprecipitated with anti-c-Myc monoclonal antibodies. Ppk18-13myc immunoprecipitates were able to phosphorylate in vitro wild-type Igo1, but not Igo1-S64A Our genetic and physiological data is in agreement with published work in budding yeast, Drosophila, Xenopus, and mammalian cells indicating that greatwall phosphorylates endo- sulfine to inhibit PP2A$B55 [8, 9, 27, 28, 35]. To test whether Igo1 is a direct target of Ppk18, we performed Ppk18 in vitro kinase assays using purified recombinant Igo1 and Igo1-S64A, as sub- strates. Extracts from wild-type (ppk18+) and Myc-tagged (ppk18-13myc) Ppk18 cells, treated for 1 hr with rapamycin in or- der to activate Ppk18, were immunoprecipitated with anti-c-Myc monoclonal antibodies. Ppk18-13myc immunoprecipitates were able to phosphorylate in vitro wild-type Igo1, but not Igo1-S64A Our genetic and physiological data is in agreement with published work in budding yeast, Drosophila, Xenopus, and mammalian cells indicating that greatwall phosphorylates endo- sulfine to inhibit PP2A$B55 [8, 9, 27, 28, 35]. To test whether Igo1 is a direct target of Ppk18, we performed Ppk18 in vitro kinase assays using purified recombinant Igo1 and Igo1-S64A, as sub- strates. Extracts from wild-type (ppk18+) and Myc-tagged (ppk18-13myc) Ppk18 cells, treated for 1 hr with rapamycin in or- der to activate Ppk18, were immunoprecipitated with anti-c-Myc monoclonal antibodies. Ppk18-13myc immunoprecipitates were able to phosphorylate in vitro wild-type Igo1, but not Igo1-S64A (Figure S4A), indicating that fission yeast Ppk18 can act as a greatwall kinase.) Phosphorylation of Igo1 was severely impaired in cells deleted for ppk18 or expressing a kinase-dead version of ppk18 (ppk18- K595A or ppk18-KD) but was still present in cek1-deleted cells (Figure 4B), consistent with the idea that Ppk18 is the main greatwall kinase that phosphorylates Igo1 in medium with low nitrogen. ------- COMMENT: 5beca4251520f38b 65 puXoE13r5ASBz5rXw2D3MvF1dkU ------- COMMENT: 5beca4251520f38b 70 1bciw6QtRuQIawCfAJBnHpumi4M again confirms other systems Endosulfines are small phosphoproteins, highly conserved from yeasts to humans, that specifically bind to and inhibit the PP2A$B55 protein phosphatase subcomplex [8, 9]. PP2A$B55 has been shown to be cell-cycle-regulated in Xenopus, following the opposite pattern of activity to Cdk1$Cyclin B (high in inter- phase and low in mitosis) [15]. To determine whether Ser64-phosphorylated Igo1 inhibits the PP2A$B55 (PP2A$Pab1 in fission yeast) phosphatase activity, we purified PP2A$Pab1 phosphatase from cells expressing GST- Pab1 using glutathione sepharose beads and assayed them for phosphatase activity. Wild-type Igo1 thiophosphorylated in vitro at Ser64 by Xenopus Greatwall, but not Igo1-S64A, in- hibited more than 90% the phosphatase activity of PP2A$Pab1 (B55) (Figures S4B and S4C). This result indicates that Ser64- phosphorylated Igo1 inhibits the activity of PP2A$B55, analogous to the situation in budding yeast [27, 28, 35] and animal cells [8, 9]. ------- COMMENT: 5beca4251520f38b 76 sMxCdl7ZifwHmUNP9nG0pKWJQ5Q (comment: MOVE EXTENSION DOWN TO NITROGEN) (comment: This can be inferred from all of the proposed EXP and is part of the proposed model, we can delete if we can make in a better way) ------- COMMENT: 5bef3e6a63b5bcf4 39 eq8SZQNuerlA3qOaETh258w4Vec To conclude, upon nutrient starvation, TORC2 functions as both an activator and an inhibitor of sexual differentiation, the latter being mediated by Taf12 phosphorylation. ------- COMMENT: 5bef3e6a63b5bcf4 117 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 5bef3e6a63b5bcf4 125 RPTNaj1867PFTO7Mi1hfRakqa1Q fig2 and supp table ------- COMMENT: 5bef3e6a63b5bcf4 126 RPTNaj1867PFTO7Mi1hfRakqa1Q fig2 and supp table ------- COMMENT: 5bef3e6a63b5bcf4 127 RPTNaj1867PFTO7Mi1hfRakqa1Q fig2 and supp table ------- COMMENT: 5bef3e6a63b5bcf4 128 RPTNaj1867PFTO7Mi1hfRakqa1Q fig2 and supp table ------- COMMENT: 5bef3e6a63b5bcf4 129 RPTNaj1867PFTO7Mi1hfRakqa1Q fig2 and supp table ------- COMMENT: 5bef3e6a63b5bcf4 130 RPTNaj1867PFTO7Mi1hfRakqa1Q fig2 and supp table ------- COMMENT: 5bef3e6a63b5bcf4 131 RPTNaj1867PFTO7Mi1hfRakqa1Q fig2 and supp table ------- COMMENT: 5bef3e6a63b5bcf4 132 RPTNaj1867PFTO7Mi1hfRakqa1Q fig2 and supp table ------- COMMENT: 5bef3e6a63b5bcf4 133 RPTNaj1867PFTO7Mi1hfRakqa1Q fig2 and supp table ------- COMMENT: 5bef3e6a63b5bcf4 134 RPTNaj1867PFTO7Mi1hfRakqa1Q fig2 and supp table ------- COMMENT: 5bef3e6a63b5bcf4 135 RPTNaj1867PFTO7Mi1hfRakqa1Q fig2 and supp table ------- COMMENT: 5bef3e6a63b5bcf4 136 RPTNaj1867PFTO7Mi1hfRakqa1Q fig2 and supp table ------- COMMENT: 5bef3e6a63b5bcf4 137 RPTNaj1867PFTO7Mi1hfRakqa1Q fig2 and supp table ------- COMMENT: 5bef3e6a63b5bcf4 138 RPTNaj1867PFTO7Mi1hfRakqa1Q fig2 and supp table ------- COMMENT: 5bef3e6a63b5bcf4 139 RPTNaj1867PFTO7Mi1hfRakqa1Q fig2 and supp table ------- COMMENT: 5bef3e6a63b5bcf4 140 RPTNaj1867PFTO7Mi1hfRakqa1Q fig2 and supp table ------- COMMENT: 5bef3e6a63b5bcf4 141 RPTNaj1867PFTO7Mi1hfRakqa1Q fig2 and supp table ------- COMMENT: 5bef3e6a63b5bcf4 142 RPTNaj1867PFTO7Mi1hfRakqa1Q fig2 and supp table ------- COMMENT: 5bef3e6a63b5bcf4 143 RPTNaj1867PFTO7Mi1hfRakqa1Q fig2 and supp table ------- COMMENT: 5bef3e6a63b5bcf4 169 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: 5bef3e6a63b5bcf4 196 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: 5bf20875f2c283f7 1 HYuBegF8XaJoFTfEe6mjyy8ueu0 assayed using bulk RNA ------- COMMENT: 5c114aae15de6314 3 fGIWzMtMZOZEPU9LLuRDGrvuXoo (comment: otr1R(SphI)::ura4+) ------- COMMENT: 5c114aae15de6314 4 fGIWzMtMZOZEPU9LLuRDGrvuXoo (comment: otr1R(SphI)::ura4+) ------- COMMENT: 5c114aae15de6314 5 fGIWzMtMZOZEPU9LLuRDGrvuXoo (comment: otr1R(SphI)::ura4+) ------- COMMENT: 5c114aae15de6314 15 mx5phPhci87sw4kvZluLpgTsRpA (comment: mat3M::ura4+) ------- COMMENT: 5c114aae15de6314 16 beb/Zn6/gV26mFQCkxu9zrUjLHI (comment: tel2L::ura4+) ------- COMMENT: 5c114aae15de6314 26 fGIWzMtMZOZEPU9LLuRDGrvuXoo (comment: otr1R(SphI)::ura4+) ------- COMMENT: 5c114aae15de6314 30 fGIWzMtMZOZEPU9LLuRDGrvuXoo (comment: otr1R(SphI)::ura4+) ------- COMMENT: 5c114aae15de6314 41 fGIWzMtMZOZEPU9LLuRDGrvuXoo (comment: otr1R(SphI)::ura4+) ------- COMMENT: 5c114aae15de6314 42 fGIWzMtMZOZEPU9LLuRDGrvuXoo (comment: otr1R(SphI)::ura4+) ------- COMMENT: 5c114aae15de6314 43 fGIWzMtMZOZEPU9LLuRDGrvuXoo (comment: otr1R(SphI)::ura4+) ------- COMMENT: 5c114aae15de6314 44 fGIWzMtMZOZEPU9LLuRDGrvuXoo (comment: otr1R(SphI)::ura4+) ------- COMMENT: 5c114aae15de6314 45 fGIWzMtMZOZEPU9LLuRDGrvuXoo (comment: otr1R(SphI)::ura4+) ------- COMMENT: 5c30b3bf5c5da95c 2 o4sWgtNABDJhlTV6TycqKFRwqmY ------- COMMENT: 5c30b3bf5c5da95c 95 T3/BsrdDJGLY2X/a9K1TMaD9deg ------- COMMENT: 5c38a2b4c0c5cd0e 3 u/FD3017yB21em/sgiOqpEvKhYw (comment: can't distinguish tlh1 and tlh2 as identical sequences) ------- COMMENT: 5c38a2b4c0c5cd0e 4 tcx2EO0IipfHadxZFdI/EVWr5iA (comment: can't distinguish tlh1 and tlh2 as identical sequences) ------- COMMENT: 5c60eca96dccd1d7 1 YE/NxlGt8+c7WgADWN4sAawi52o Inhibition of Dsk1 and Prp4 caused a significant increase of intron retention in 1,945 (~42%) and 2,148 splicing events (~47%), respectively, thus indicating broad defects in RNA splicing (Fig. 1b). I ------- COMMENT: 5c60eca96dccd1d7 2 YE/NxlGt8+c7WgADWN4sAawi52o Inhibition of Dsk1 and Prp4 caused a significant increase of intron retention in 1,945 (~42%) and 2,148 splicing events (~47%), respectively, thus indicating broad defects in RNA splicing (Fig. 1b). I ------- COMMENT: 5c60eca96dccd1d7 3 4j8nCAfFMKItFjrXJ2mGiujcQrk Figure 2 SR protein kinases use an evolutionarily conserved RXXSP motif for substrate recognition ------- COMMENT: 5c60eca96dccd1d7 4 4j8nCAfFMKItFjrXJ2mGiujcQrk Figure 2 SR protein kinases use an evolutionarily conserved RXXSP motif for substrate recognition ------- COMMENT: 5c60eca96dccd1d7 5 4j8nCAfFMKItFjrXJ2mGiujcQrk Figure 2 SR protein kinases use an evolutionarily conserved RXXSP motif for substrate recognition ------- COMMENT: 5c60eca96dccd1d7 6 4j8nCAfFMKItFjrXJ2mGiujcQrk Figure 2 SR protein kinases use an evolutionarily conserved RXXSP motif for substrate recognition ------- COMMENT: 5c60eca96dccd1d7 7 4j8nCAfFMKItFjrXJ2mGiujcQrk Figure 2 SR protein kinases use an evolutionarily conserved RXXSP motif for substrate recognition ------- COMMENT: 5c60eca96dccd1d7 8 4j8nCAfFMKItFjrXJ2mGiujcQrk Figure 2 SR protein kinases use an evolutionarily conserved RXXSP motif for substrate recognition ------- COMMENT: 5c60eca96dccd1d7 9 4j8nCAfFMKItFjrXJ2mGiujcQrk Figure 2 SR protein kinases use an evolutionarily conserved RXXSP motif for substrate recognition ------- COMMENT: 5c60eca96dccd1d7 10 4j8nCAfFMKItFjrXJ2mGiujcQrk Figure 2 SR protein kinases use an evolutionarily conserved RXXSP motif for substrate recognition ------- COMMENT: 5c60eca96dccd1d7 11 4j8nCAfFMKItFjrXJ2mGiujcQrk Figure 2 SR protein kinases use an evolutionarily conserved RXXSP motif for substrate recognition ------- COMMENT: 5c60eca96dccd1d7 12 4j8nCAfFMKItFjrXJ2mGiujcQrk Figure 2 SR protein kinases use an evolutionarily conserved RXXSP motif for substrate recognition ------- COMMENT: 5c60eca96dccd1d7 13 4j8nCAfFMKItFjrXJ2mGiujcQrk Figure 2 SR protein kinases use an evolutionarily conserved RXXSP motif for substrate recognition ------- COMMENT: 5c60eca96dccd1d7 14 4j8nCAfFMKItFjrXJ2mGiujcQrk Figure 2 SR protein kinases use an evolutionarily conserved RXXSP motif for substrate recognition ------- COMMENT: 5c60eca96dccd1d7 15 4j8nCAfFMKItFjrXJ2mGiujcQrk Figure 2 SR protein kinases use an evolutionarily conserved RXXSP motif for substrate recognition ------- COMMENT: 5c60eca96dccd1d7 16 4j8nCAfFMKItFjrXJ2mGiujcQrk Figure 2 SR protein kinases use an evolutionarily conserved RXXSP motif for substrate recognition ------- COMMENT: 5c60eca96dccd1d7 18 EJR7sLx+SHAVbAO1oUh1BnFq+Sc We conclude that phosphorylation of the Dsk1 substrate Bpb1 has a major effect on the genome-wide splicing pattern. ------- COMMENT: 5c60eca96dccd1d7 48 k9zaM0te95O6gX7u12qJ4YGmdaQ However, mutation of the conserved S131 and S133 residues to alanine in Bpb1 (denoted Bpb1-2A) caused significant intron retention in 1,598 introns (~35%) (Fig. 3b) and thus produces a defect quantitatively and qualitatively similar to inhibition of either Dsk1 (ρ = 0.62 ± 0.02; P < 10−6, two sided) or Prp4 (ρ = 0.63 ± 0.02; P < 10−6, two sided). ------- COMMENT: 5c60eca96dccd1d7 50 WS9P+x5K5plYGHSemOMdiDv4tVw Importantly, introns retained in a temperature-sensitive mutant of the S. pombe U2AF65 ortholog prp2 (prp2-1) (2,873 introns retained, ~62%) (Supplementary Fig. 3b) did not result in a consistent positive correlation with suboptimal cis-regulatory sequences (Fig. 4a and Supplementary Fig. 3a). ------- COMMENT: 5c60eca96dccd1d7 52 0xk9aqUXwgIzh0qKVKrEINkQnAQ Dsk1 readily phosphorylated WT Bpb1 in vitro, and mutation of Bpb1 at either S131 or S133 to alanine did not abolish phosphorylation (Fig. 5a). However, mutation of both S131 and S133 did, thus indicating that these two residues are the major sites of Bpb1 phosphorylation by Dsk1 (Fig. 5a). ------- COMMENT: 5c60eca96dccd1d7 53 0xk9aqUXwgIzh0qKVKrEINkQnAQ Dsk1 readily phosphorylated WT Bpb1 in vitro, and mutation of Bpb1 at either S131 or S133 to alanine did not abolish phosphorylation (Fig. 5a). However, mutation of both S131 and S133 did, thus indicating that these two residues are the major sites of Bpb1 phosphorylation by Dsk1 (Fig. 5a). ------- COMMENT: 5c6727a02c994591 2 SKBpTeylpNmb8ssVYSSNwvSnTUU Explosive cell separation due to a weak primary septum. Absence of a secondary septum. ------- COMMENT: 5c6727a02c994591 3 4sJId6qcFvGXdX/R3DnLBCMME1M Localized at cell tips, actomyosin contractile ring and septum ------- COMMENT: 5c6727a02c994591 17 5bPplDQL4ovTx/lqHjLTGH9RND8 fig1 ------- COMMENT: 5c6727a02c994591 19 5bPplDQL4ovTx/lqHjLTGH9RND8 fig1 ------- COMMENT: 5c744b7dd350f431 13 N6yUChjFerE+gXkgIbBsQCWnwHE AA medium (Rose et al 1990 Methods in Yeast Genetics) ------- COMMENT: 5c744b7dd350f431 14 N6yUChjFerE+gXkgIbBsQCWnwHE AA medium (Rose et al 1990 Methods in Yeast Genetics) ------- COMMENT: 5c8e5c615d0b3d1d 2 tA60vsAnJusuV32bdx0hE60lK2Q (comment: At RRM3 motif) ------- COMMENT: 5cba71fc62d1cc66 1 p3x2dDTjQlS9jbO+IMHpHbED4nw (Fig. 1D) ------- COMMENT: 5cba71fc62d1cc66 2 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: 5cba71fc62d1cc66 3 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: 5cba71fc62d1cc66 4 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: 5cba71fc62d1cc66 5 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 5cba71fc62d1cc66 6 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: 5cba71fc62d1cc66 8 hPFNK9pXn9s6uyKJPTkg3DIM2XE (Fig. 6A) ------- COMMENT: 5cba71fc62d1cc66 9 9obkRBahnsvfuKPq+LTlya+asC4 (Fig. 6E) ------- COMMENT: 5cba71fc62d1cc66 10 9obkRBahnsvfuKPq+LTlya+asC4 (Fig. 6E) ------- COMMENT: 5cba71fc62d1cc66 11 wnx2gS3IAKqzPkStlyJ0uF9GveM (Fig. 6C) ------- COMMENT: 5cba71fc62d1cc66 12 XwjFKIBpFnAfCL+3uv8ZHt+X4Cc (Fig. 6D) ------- COMMENT: 5cba71fc62d1cc66 13 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: 5cba71fc62d1cc66 14 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: 5cba71fc62d1cc66 15 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: 5cba71fc62d1cc66 16 kb7NOBRiqlmzY3vQrPFeR5NkglI (Fig. 7C) ------- COMMENT: 5cba71fc62d1cc66 17 vGtIAlSSPNnMrmPTCV9WpCBeLHE (Fig. 7B) ------- COMMENT: 5cba71fc62d1cc66 18 vGtIAlSSPNnMrmPTCV9WpCBeLHE (Fig. 7B) ------- COMMENT: 5cba71fc62d1cc66 19 vGtIAlSSPNnMrmPTCV9WpCBeLHE (Fig. 7B) ------- COMMENT: 5cba71fc62d1cc66 20 p9PqWaxu79GLWhKfMyOCtz3437I (Fig. 7F) ------- COMMENT: 5cba71fc62d1cc66 21 YSfvpxZxXYWLkz1N2bWoK/o2ZgM (Fig. 7F and G) ------- COMMENT: 5cba71fc62d1cc66 22 bKUVPFcRiS0e2ZeZ4ffj9xKhmMg (Fig. 7H) ------- COMMENT: 5cba71fc62d1cc66 23 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: 5cba71fc62d1cc66 24 wnx2gS3IAKqzPkStlyJ0uF9GveM (Fig. 6C) ------- COMMENT: 5cba71fc62d1cc66 25 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: 5ce1860d8c2a56ac 1 /lopHkWuH5HMvf/jMooGtPYQEw4 supp 1A ------- COMMENT: 5ce1860d8c2a56ac 2 /lopHkWuH5HMvf/jMooGtPYQEw4 supp 1A ------- COMMENT: 5ce1860d8c2a56ac 3 /lopHkWuH5HMvf/jMooGtPYQEw4 supp 1A ------- COMMENT: 5ce1860d8c2a56ac 4 /lopHkWuH5HMvf/jMooGtPYQEw4 supp 1A ------- COMMENT: 5ce1860d8c2a56ac 5 M+IKfspNBFFAXdmAsylXIKzR4UM (Figure 1A, right panel). Figure 1C ------- COMMENT: 5ce1860d8c2a56ac 6 yVSverZnGIFe7p1VA8f4SwDBaaY (Figure 1B, left panel), ------- COMMENT: 5ce1860d8c2a56ac 7 yVSverZnGIFe7p1VA8f4SwDBaaY (Figure 1B, left panel), ------- COMMENT: 5ce1860d8c2a56ac 8 yVSverZnGIFe7p1VA8f4SwDBaaY (Figure 1B, left panel), ------- COMMENT: 5ce1860d8c2a56ac 9 M+IKfspNBFFAXdmAsylXIKzR4UM (Figure 1A, right panel). Figure 1C ------- COMMENT: 5ce1860d8c2a56ac 10 M+IKfspNBFFAXdmAsylXIKzR4UM (Figure 1A, right panel). Figure 1C ------- COMMENT: 5ce1860d8c2a56ac 11 LP/6jS9PYQba9xbyFI5h83asPkU (comment: to cell division site) We confirmed that the actomyosin ring structure was important for polarization of the early secretory compartments by examiningthe spatial distribution of the tER and Golgi compartmentsin cells with compromised function of the myosin lightchainCdc4p ------- COMMENT: 5ce1860d8c2a56ac 12 PS0JRqqVoHI89AjqVuB3afAlWeg (comment: CHECK abnormal ER polarization (ectopic)) ------- COMMENT: 5ce1860d8c2a56ac 14 wXC2pj9SXfESaFc6BRtd8m3UG5M (comment: CHECK abnormal ER polarization) We observed a striking reduction in the number of cells exhibiting clear polarization of the tER in cdc15-140 cells already at the permissive temperature of 24°C (Figure 5A, bottom panel), ------- COMMENT: 5d43b3c8107ba0de 1 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 5d43b3c8107ba0de 2 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 5d43b3c8107ba0de 3 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 5d43b3c8107ba0de 4 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 5d43b3c8107ba0de 5 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 5d43b3c8107ba0de 6 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 5d43b3c8107ba0de 7 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 5d43b3c8107ba0de 8 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 5d43b3c8107ba0de 9 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 5d8d5a6ec633fc68 1 larMeJ0ZmSbSnce0U20QXrb7a2w Figure 2D&E ------- COMMENT: 5d8d5a6ec633fc68 2 uQuiDc1ij0S+9yYrXwJ1JSjJGuU Figure 2F-H ------- COMMENT: 5d8d5a6ec633fc68 3 RdpWCLIx7A4JtU2z40FYsGmx8s0 Figure 2I ------- COMMENT: 5d8d5a6ec633fc68 17 FZydZXsCDMDUXo7UlIK8k9UkoZY Fig S3B&C ------- COMMENT: 5d8d5a6ec633fc68 18 FZydZXsCDMDUXo7UlIK8k9UkoZY Fig S3B&C ------- COMMENT: 5d8d5a6ec633fc68 19 5kNTcf30ZQYPe0VmspYEHkx8gng Fig S3F ------- COMMENT: 5d8d5a6ec633fc68 20 8YayFVz5JLN0BH41NcYoLEy69oE Fig S3F&G ------- COMMENT: 5d8d5a6ec633fc68 21 UvLHD8qMT9ZjpsqKF1uZVxB8Sgg Fig S3B,C,&D ------- COMMENT: 5d8d5a6ec633fc68 22 DkRlm96Z4MUsJY1lnB5yHkYIQEs Fig 4D,E&F ------- COMMENT: 5d8d5a6ec633fc68 23 68No6TamK8CxqZsgCxyyJBnRdHQ Fig S3E ------- COMMENT: 5d8d5a6ec633fc68 24 68No6TamK8CxqZsgCxyyJBnRdHQ Fig S3E ------- COMMENT: 5d8d5a6ec633fc68 25 68No6TamK8CxqZsgCxyyJBnRdHQ Fig S3E ------- COMMENT: 5d8d5a6ec633fc68 26 68No6TamK8CxqZsgCxyyJBnRdHQ Fig S3E ------- COMMENT: 5d8d5a6ec633fc68 27 cljC8cTt4iMJbVDTiL2Nq9An390 Fig 4H&I and S3F&G ------- COMMENT: 5d8d5a6ec633fc68 28 cljC8cTt4iMJbVDTiL2Nq9An390 Fig 4H&I and S3F&G ------- COMMENT: 5d8d5a6ec633fc68 31 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 5d8d5a6ec633fc68 35 Xb1L1D0oeozHWjRnmVjhdBSDo98 Fig. S3A ------- COMMENT: 5d8d5a6ec633fc68 36 Xb1L1D0oeozHWjRnmVjhdBSDo98 Fig. S3A ------- COMMENT: 5d8d5a6ec633fc68 37 Xb1L1D0oeozHWjRnmVjhdBSDo98 Fig. S3A ------- COMMENT: 5d8d5a6ec633fc68 38 Xb1L1D0oeozHWjRnmVjhdBSDo98 Fig. S3A ------- COMMENT: 5d8d5a6ec633fc68 41 iugwOICngLmkyma0auuN7ZPWnmQ Fig 3D ------- COMMENT: 5d8d5a6ec633fc68 42 iugwOICngLmkyma0auuN7ZPWnmQ Fig 3D ------- COMMENT: 5d8d5a6ec633fc68 43 iugwOICngLmkyma0auuN7ZPWnmQ Fig 3D ------- COMMENT: 5d95c2f1713ccd8c 14 Sg+sP40i2xExikeyr6CKhjnWc4k (comment: tested through observing no delay when checkpoint is inactivated) ------- COMMENT: 5d9d97db30265d3a 1 U+BqKwfufIivUqMyjgoLCKwwAwQ (comment: Dnt1 down-regulates Wee1 kinase. Had to remove extensions: independent_of(PomBase:Cdc25)| independent_of(PomBase:Rad3)| independent_of(PomBase:Chk1)| independent_of(PomBase:Cds1)| independent_of(PomBase:Clp1)| independent_of(PomBase:Pom1)| independent_of(PomBase:Cut12)| dependent_on(PomBase:Wee1) | acts_upstream_of(wee1)) ------- COMMENT: 5d9d97db30265d3a 15 ENHtswefVeWUwhqgZvdf2nAoENU (comment: wee1-50 epistatic to dnt1delta; shows that cell cycle regulation by Dnt1 depends on Wee1) ------- COMMENT: 5da0f1305d21c994 1 ewfspod7Bin5Lx5/B/NV8EarpfI (comment: CONDITION 30 degrees) ------- COMMENT: 5da0f1305d21c994 3 ewfspod7Bin5Lx5/B/NV8EarpfI (comment: CONDITION 30 degrees) ------- COMMENT: 5da0f1305d21c994 4 max4HEZNUxQSXwmzYVBOGtNvtHw (comment: CONDITION 25 degrees) ------- COMMENT: 5da0f1305d21c994 14 XhRnG+5R7RyXrmiQAl3DeKjRPn4 (comment: MBP substrate) ------- COMMENT: 5da0f1305d21c994 25 hG2daKFoTntWUQ2eI6+eCPKM/SI (comment: same as hsk1-89 alone) ------- COMMENT: 5da0f1305d21c994 26 hG2daKFoTntWUQ2eI6+eCPKM/SI (comment: same as hsk1-89 alone) ------- COMMENT: 5da0f1305d21c994 28 hG2daKFoTntWUQ2eI6+eCPKM/SI (comment: same as hsk1-89 alone) ------- COMMENT: 5da0f1305d21c994 29 ewfspod7Bin5Lx5/B/NV8EarpfI (comment: CONDITION 30 degrees) ------- COMMENT: 5da0f1305d21c994 30 max4HEZNUxQSXwmzYVBOGtNvtHw (comment: CONDITION 25 degrees) ------- COMMENT: 5da0f1305d21c994 31 v3aPkj0z4DxYwX8Y48XndNN5OTw (comment: CONDITION 30 degrees) (comment: same as hsk1-89 alone) ------- COMMENT: 5da0f1305d21c994 32 USGRbmWcGn46pIyd9Vg60qj2TQI (comment: CONDITION 25 degrees) (comment: same as hsk1-89 alone) ------- COMMENT: 5da0f1305d21c994 33 fmRN4M0SFn06WyWlbeFPQzsv4Kg (comment: CONDITION 30 degrees) restrictive for hsk1-89 alone ------- COMMENT: 5da0f1305d21c994 34 v3aPkj0z4DxYwX8Y48XndNN5OTw (comment: CONDITION 30 degrees) (comment: same as hsk1-89 alone) ------- COMMENT: 5da0f1305d21c994 35 USGRbmWcGn46pIyd9Vg60qj2TQI (comment: CONDITION 25 degrees) (comment: same as hsk1-89 alone ------- COMMENT: 5da0f1305d21c994 36 v3aPkj0z4DxYwX8Y48XndNN5OTw (comment: CONDITION 30 degrees) (comment: same as hsk1-89 alone) ------- COMMENT: 5da0f1305d21c994 37 USGRbmWcGn46pIyd9Vg60qj2TQI (comment: CONDITION 25 degrees) (comment: same as hsk1-89 alone) ------- COMMENT: 5da0f1305d21c994 38 v3aPkj0z4DxYwX8Y48XndNN5OTw (comment: CONDITION 30 degrees) (comment: same as hsk1-89 alone) ------- COMMENT: 5da0f1305d21c994 39 USGRbmWcGn46pIyd9Vg60qj2TQI (comment: CONDITION 25 degrees) (comment: same as hsk1-89 alone) ------- COMMENT: 5da0f1305d21c994 42 ND9H3aixpVsolVUfsYUpAcVTiyY (comment: in SQ/TQ clusters) (comment: CHECK activated_by(CHEBI:29035)) ------- COMMENT: 5da0f1305d21c994 43 cfGDB+mjcETzXNvrj8nqrpdixoo (comment: not in SQ/TQ clusters) ------- COMMENT: 5da0f1305d21c994 44 IljQ4RbK8PU358bc0CaWOKqxla8 (comment: at ars2004 and oriChr2-1266, during early S phase) ------- COMMENT: 5da0f1305d21c994 47 FyfTBQuIFjV+/q6gqsQ4DSDrhmE (comment: hsk1 phenotypes more informative than mrc1 itself) ------- COMMENT: 5da5597d14f0cc49 1 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 5da5597d14f0cc49 2 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 5da5597d14f0cc49 3 5DWqo03puFeQtodWqcCbmJ6nfyM fig 1A ------- COMMENT: 5da5597d14f0cc49 4 5DWqo03puFeQtodWqcCbmJ6nfyM fig 1A ------- COMMENT: 5da5597d14f0cc49 5 5DWqo03puFeQtodWqcCbmJ6nfyM fig 1A ------- COMMENT: 5da5597d14f0cc49 6 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 5da5597d14f0cc49 7 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 5da5597d14f0cc49 9 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 5da5597d14f0cc49 10 pcd9kSpPV6dG7CGeBK50geNYWR0 fig 1c/d ------- COMMENT: 5da5597d14f0cc49 11 KjDNy3faq4c7K8BwU9YbHvIuFic fig 2 a/b ------- COMMENT: 5da5597d14f0cc49 12 KjDNy3faq4c7K8BwU9YbHvIuFic fig 2 a/b ------- COMMENT: 5da5597d14f0cc49 13 KjDNy3faq4c7K8BwU9YbHvIuFic fig 2 a/b ------- COMMENT: 5da5597d14f0cc49 17 cWU1gxrBhSdpW25jqWFTdTZ9qBA fig 3A ------- COMMENT: 5da5597d14f0cc49 18 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: 5da5597d14f0cc49 19 23A7gKYH++mwxpDaLPxFOcAYx6Y fig 3c ------- COMMENT: 5da5597d14f0cc49 20 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: 5da5597d14f0cc49 21 pwcwASlWQtRBBoormzj5gHYObdE fig 4A ------- COMMENT: 5da5597d14f0cc49 22 pwcwASlWQtRBBoormzj5gHYObdE fig 4A ------- COMMENT: 5da5597d14f0cc49 23 pwcwASlWQtRBBoormzj5gHYObdE fig 4A ------- COMMENT: 5da5597d14f0cc49 24 pwcwASlWQtRBBoormzj5gHYObdE fig 4A ------- COMMENT: 5da5597d14f0cc49 25 //QleQcD3kkWGdAJo2oN7LSuYAA fig 4B ------- COMMENT: 5da5597d14f0cc49 26 //QleQcD3kkWGdAJo2oN7LSuYAA fig 4B ------- COMMENT: 5da5597d14f0cc49 27 9QQ7HthEXp/JWeCIYT7IquPfxAo fig 4C ------- COMMENT: 5da5597d14f0cc49 35 hs/Tf59vskhfJUoX4Z1YwSn9CFU (comment: CHECK not sure if this is correct....) ------- COMMENT: 5da5597d14f0cc49 36 hs/Tf59vskhfJUoX4Z1YwSn9CFU (comment: CHECK not sure if this is correct....) ------- COMMENT: 5da5597d14f0cc49 37 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 5de6e47f8f999ca9 27 gD3IeiTuN78ceH2onvO1UuREChw (comment: full-length Rad3 or Rad3-kd-delta) ------- COMMENT: 5de6e47f8f999ca9 30 gD3IeiTuN78ceH2onvO1UuREChw (comment: CHECK full-length Rad3 or Rad3-kd-delta) ------- COMMENT: 5de6e47f8f999ca9 51 qmU/AUQNBz9NZDTKJIYxuZePY2A (comment: same as nbs1-c60-delta alone) ------- COMMENT: 5de6e47f8f999ca9 60 qmU/AUQNBz9NZDTKJIYxuZePY2A (comment: same as nbs1-c60-delta alone) ------- COMMENT: 5e17d086a1ce0097 1 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 5e1ab5b7f5ffc7d5 9 uDbx17S/VH1PtcH8L6p62nagOxs lower affinity than for Y-form DNA ------- COMMENT: 5e1ab5b7f5ffc7d5 10 hNexUzlhJk81FG6TQOmzaJQ4rQ0 ------- COMMENT: 5e219fdc549363e4 12 cW1DYNm++NCbnFfIaW1na3aNi9E ------- COMMENT: 5e362e900b991f0d 2 SiOw4kwZwroRY1GIvdIN7x3nk+M cells stop growing at high temperature, but remain viable and resume growth and division when returned to standard temperature ------- COMMENT: 5e362e900b991f0d 3 SiOw4kwZwroRY1GIvdIN7x3nk+M cells stop growing at high temperature, but remain viable and resume growth and division when returned to standard temperature ------- COMMENT: 5e362e900b991f0d 18 rvln7MQguQ+2hS5PE4aguxhBiVk TORC1 senses nutrients and pushes cells into the next cell cycle. Removing TORC1 activity, you get a buildup of rum1 and srw1 which inhibit G1/S ------- COMMENT: 5e4169b314b92367 1 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 5e4169b314b92367 2 mvls1XRkifPit0YRxwvURn4ff6Y Figure 1B. From this screen we observed one significant interaction: fic1’s phospho-ablating mutant, fic1-2A, suppressed myo2-E1 ------- COMMENT: 5e4169b314b92367 3 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 5e4169b314b92367 4 /cZODLS8WnVbpKN5OgwtpUmiX9U Figure 1E and G ------- COMMENT: 5e4169b314b92367 5 YRAg0qZaDg3tUEeuUtocqa+T4MY Figure 1 E and G ------- COMMENT: 5e4169b314b92367 6 /cZODLS8WnVbpKN5OgwtpUmiX9U Figure 1E and G ------- COMMENT: 5e4169b314b92367 7 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 5e4169b314b92367 8 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 5e4169b314b92367 9 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 5e4169b314b92367 10 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 5e4169b314b92367 11 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 5e4169b314b92367 12 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 5e4169b314b92367 13 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 5e4169b314b92367 14 pkVv0ur7uKV1nM+rtV7gf1516fI Figure 2 Fig. 2. fic1-2A myo2-E1 cells can achieve membrane ingression and cell separation at myo2-E1’s restrictive temperature ------- COMMENT: 5e4169b314b92367 15 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 5e4169b314b92367 16 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 5e4169b314b92367 17 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 5e4169b314b92367 18 LQmuKZbL9ND56pzzWctDT58tWRE Figure 3C ------- COMMENT: 5e4169b314b92367 19 LQmuKZbL9ND56pzzWctDT58tWRE Figure 3C ------- COMMENT: 5e4169b314b92367 20 LQmuKZbL9ND56pzzWctDT58tWRE Figure 3C ------- COMMENT: 5e4169b314b92367 21 ZbDhwGSYZQV+P9ZAEmzZiFsKpi0 Figure 3C We next asked if S. pombe Cyk3 was required for fic1-2A’s suppression of myo2-E1. Indeed, cyk3Δ prevented fic1-2A from suppressing myo2-E1 ------- COMMENT: 5e4169b314b92367 24 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: 5e4169b314b92367 25 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: 5e4169b314b92367 26 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: 5e4169b314b92367 27 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: 5e4169b314b92367 28 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: 5e4169b314b92367 29 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: 5e4169b314b92367 30 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: 5e4169b314b92367 31 b+00gXuAJVmG6MoHxSub6s9WxVw Figure 4C ------- COMMENT: 5e4169b314b92367 32 b+00gXuAJVmG6MoHxSub6s9WxVw Figure 4C ------- COMMENT: 5e4169b314b92367 33 b+00gXuAJVmG6MoHxSub6s9WxVw Figure 4C ------- COMMENT: 5e4169b314b92367 34 b+00gXuAJVmG6MoHxSub6s9WxVw Figure 4C ------- COMMENT: 5e4169b314b92367 35 b+00gXuAJVmG6MoHxSub6s9WxVw Figure 4C ------- COMMENT: 5e4169b314b92367 36 b+00gXuAJVmG6MoHxSub6s9WxVw Figure 4C ------- COMMENT: 5e4169b314b92367 37 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: 5e4169b314b92367 38 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: 5e4169b314b92367 39 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: 5e4169b314b92367 40 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 5e4169b314b92367 41 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 5e4169b314b92367 42 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 5e4169b314b92367 43 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 5e4169b314b92367 44 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 5e4169b314b92367 45 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 5e4169b314b92367 46 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 5e4169b314b92367 47 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: 5e4169b314b92367 48 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: 5e4169b314b92367 49 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: 5e4169b314b92367 50 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: 5e4169b314b92367 51 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 5e4169b314b92367 52 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 5e4169b314b92367 53 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 5e4169b314b92367 54 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 5e4169b314b92367 55 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 5e4169b314b92367 56 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 5e4169b314b92367 57 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 5e4169b314b92367 58 aQqj/TwRKA7VIttZbYxg0/YnElU From this screen we observed one significant interaction: fic1’s phospho-ablating mutant, fic1-2A, suppressed myo2-E1 ------- COMMENT: 5e4169b314b92367 60 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 5e4169b314b92367 61 /cZODLS8WnVbpKN5OgwtpUmiX9U Figure 1E and G ------- COMMENT: 5e4169b314b92367 62 LQmuKZbL9ND56pzzWctDT58tWRE Figure 3C ------- COMMENT: 5e4169b314b92367 63 LQmuKZbL9ND56pzzWctDT58tWRE Figure 3C ------- COMMENT: 5e4169b314b92367 64 ZbDhwGSYZQV+P9ZAEmzZiFsKpi0 Figure 3C We next asked if S. pombe Cyk3 was required for fic1-2A’s suppression of myo2-E1. Indeed, cyk3Δ prevented fic1-2A from suppressing myo2-E1 ------- COMMENT: 5e5f2a97efafec91 2 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 5e5f2a97efafec91 3 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 5e5f2a97efafec91 5 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 5e5f2a97efafec91 7 dsjhtWRvx2+KfFD3myPwALjHhJI unpublished obsevation ------- COMMENT: 5e5f2a97efafec91 8 dsjhtWRvx2+KfFD3myPwALjHhJI unpublished obsevation ------- COMMENT: 5e5f2a97efafec91 9 j8lx0cjXOogtvtAy6trm2XWxJ9Q fig2a ------- COMMENT: 5e5f2a97efafec91 10 k1BouxSkKSUbH+29howqPIOVQSo Fig. 3A, lanes 1 and 7 ------- COMMENT: 5e5f2a97efafec91 11 oj/b9dKAXRBG44AJRgFPqBG9I8c Fig. 3A, lanes 1 and 7 (control) ------- COMMENT: 5e5f2a97efafec91 12 Ag6GoP3zsrPsg4IZ5Mmwasgbws4 Fig3 ------- COMMENT: 5e5f2a97efafec91 13 Ag6GoP3zsrPsg4IZ5Mmwasgbws4 Fig3 ------- COMMENT: 5e5f2a97efafec91 14 Ag6GoP3zsrPsg4IZ5Mmwasgbws4 Fig3 ------- COMMENT: 5e5f2a97efafec91 15 Ag6GoP3zsrPsg4IZ5Mmwasgbws4 Fig3 ------- COMMENT: 5e5f2a97efafec91 16 YBJpGerGY/Gc1zFX7+1Llqx4r1U Fig. 3C ------- COMMENT: 5e5f2a97efafec91 17 YBJpGerGY/Gc1zFX7+1Llqx4r1U Fig. 3C ------- COMMENT: 5e5f2a97efafec91 18 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 5e5f2a97efafec91 19 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 5e5f2a97efafec91 20 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 5e5f2a97efafec91 21 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 5e5f2a97efafec91 23 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 5e5f2a97efafec91 24 CST2MRdbW/+GskNaKjpN81PKKJw fig2a colocalizes with sep3 ------- COMMENT: 5e5f2a97efafec91 26 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 5e5f2a97efafec91 27 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 5e5f2a97efafec91 28 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 5e5f2a97efafec91 29 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 5e5f2a97efafec91 30 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 5e5f2a97efafec91 31 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 5e5f2a97efafec91 32 xrnc8yKTfw/T29zPEjGa7ZRaBeM Fig. 7D ------- COMMENT: 5e5f2a97efafec91 33 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 5e5f2a97efafec91 35 4R+lBpYtzgzk2vhxC1Wq1WZ0svw Fig8 ------- COMMENT: 5e5f2a97efafec91 36 4R+lBpYtzgzk2vhxC1Wq1WZ0svw Fig8 ------- COMMENT: 5e5f2a97efafec91 37 4R+lBpYtzgzk2vhxC1Wq1WZ0svw Fig8 ------- COMMENT: 5e5f2a97efafec91 38 4R+lBpYtzgzk2vhxC1Wq1WZ0svw Fig8 ------- COMMENT: 5e5f2a97efafec91 39 4R+lBpYtzgzk2vhxC1Wq1WZ0svw Fig8 ------- COMMENT: 5e8e362b7dec8c54 26 e05PtyfdQCNE8/MyZxEiJpXvmqI Ifh1 was co-immunoprecipitated with Fhl1 both in nutrient-rich and nutrient-starved conditions (Fig. 4D), ------- COMMENT: 5e8e362b7dec8c54 27 tMNvPGS1yvfZplz7gpmTPnx7dSs The screen isolated a plasmid, pALSK53, which suppressed the ts growth phenotype and rapamycin sensitivity of the tor2-13 mutant (Fig. 1A). ------- COMMENT: 5e8e362b7dec8c54 28 tMNvPGS1yvfZplz7gpmTPnx7dSs The screen isolated a plasmid, pALSK53, which suppressed the ts growth phenotype and rapamycin sensitivity of the tor2-13 mutant (Fig. 1A). ------- COMMENT: 5e8e362b7dec8c54 29 NBxE1TMORqWLATs2eTl9KTbKCTs Truncation of amino acids 84 to 105 or amino acids 293 to 442 of Sfp1, which removes the N-terminal or C-terminal zinc finger domains, resulted in reduced growth defect suppression in the tor2-287 mutant compared to full-length Sfp1 (Fig. 1D). ------- COMMENT: 5e8e362b7dec8c54 30 NBxE1TMORqWLATs2eTl9KTbKCTs Truncation of amino acids 84 to 105 or amino acids 293 to 442 of Sfp1, which removes the N-terminal or C-terminal zinc finger domains, resulted in reduced growth defect suppression in the tor2-287 mutant compared to full-length Sfp1 (Fig. 1D). ------- COMMENT: 5e8e362b7dec8c54 31 KiAKmFFBeia/c/YJO749fRtxYhY The level of Psk1 phosphorylation was comparable between wild-type and tor2-287 cells growing at 25  C (Fig. 1E). Upon temperature shift to the restrictive temperature, dephosphorylation of Psk1 was observed with similar kinetics both in the presence and absence of Sfp1 overexpression (Fig. 1E), suggesting that Sfp1 does not affect TORC1 activity. ------- COMMENT: 5e8e362b7dec8c54 32 YGMRLfxxIeyE4/6wYQ3RiMY0Bi8 TORC1 activity monitored by the Psk1 phosphorylation was comparable between wild-type and Dsfp1 cells before and after nitrogen starvation (Fig. 1F), further confirming that Sfp1 does not affect TORC1 activity. ------- COMMENT: 5e8e362b7dec8c54 33 VE1MhJ80BEDklBBt0/86AbZqVrY In contrast, the Dsfp1 mutant exhibited a modest growth defect as well as limited rapamycin sensitivity (Fig. 1G), consistent with the notion that Sfp1 is involved in cell proliferation regulated by TORC1. ------- COMMENT: 5e8e362b7dec8c54 34 cQGkOlO9vban5YKch/ryzvG9dtA Although the growth defect of the Dsfp1 mutant was observed on glucose medium, it grew normally on medium containing glycerol as the carbon source (Fig. 1H), similar to the situation in budding yeast.27 ------- COMMENT: 5e8e362b7dec8c54 35 dvk6lVy8GLS/UYEkEP/0UuT0/m4 Although the growth defect of the Dsfp1 mutant was observed on glucose medium, it grew normally on medium containing glycerol as the carbon source (Fig. 1H), similar to the situation in budding yeast.27. ALSO figure 4B for severity ------- COMMENT: 5e8e362b7dec8c54 36 HzYIqxL4E02OnjwlcTakuNB1XoA Notably, the absence of Sfp1 modestly alleviates the growth defect of the mutants lacking functional GATOR1 (Fig. 1I); the absence of GATOR1 causes a severe growth defect due to deregulated TORC1 activation,28 and therefore, the observed genetic interaction corroborates a functional link between Sfp1 and TORC1. ------- COMMENT: 5e8e362b7dec8c54 37 HzYIqxL4E02OnjwlcTakuNB1XoA Notably, the absence of Sfp1 modestly alleviates the growth defect of the mutants lacking functional GATOR1 (Fig. 1I); the absence of GATOR1 causes a severe growth defect due to deregulated TORC1 activation,28 and therefore, the observed genetic interaction corroborates a functional link between Sfp1 and TORC1. ------- COMMENT: 5e8e362b7dec8c54 38 HzYIqxL4E02OnjwlcTakuNB1XoA Notably, the absence of Sfp1 modestly alleviates the growth defect of the mutants lacking functional GATOR1 (Fig. 1I); the absence of GATOR1 causes a severe growth defect due to deregulated TORC1 activation,28 and therefore, the observed genetic interaction corroborates a functional link between Sfp1 and TORC1. ------- COMMENT: 5e8e362b7dec8c54 39 HzYIqxL4E02OnjwlcTakuNB1XoA Notably, the absence of Sfp1 modestly alleviates the growth defect of the mutants lacking functional GATOR1 (Fig. 1I); the absence of GATOR1 causes a severe growth defect due to deregulated TORC1 activation,28 and therefore, the observed genetic interaction corroborates a functional link between Sfp1 and TORC1. ------- COMMENT: 5e8e362b7dec8c54 40 HzYIqxL4E02OnjwlcTakuNB1XoA Notably, the absence of Sfp1 modestly alleviates the growth defect of the mutants lacking functional GATOR1 (Fig. 1I); the absence of GATOR1 causes a severe growth defect due to deregulated TORC1 activation,28 and therefore, the observed genetic interaction corroborates a functional link between Sfp1 and TORC1. ------- COMMENT: 5e8e362b7dec8c54 41 HzYIqxL4E02OnjwlcTakuNB1XoA Notably, the absence of Sfp1 modestly alleviates the growth defect of the mutants lacking functional GATOR1 (Fig. 1I); the absence of GATOR1 causes a severe growth defect due to deregulated TORC1 activation,28 and therefore, the observed genetic interaction corroborates a functional link between Sfp1 and TORC1. ------- COMMENT: 5e8e362b7dec8c54 42 gdlGN3cwt55aZvZ9pV8po6bQhnE In the tor2-287 and tor2-13 mutants, the Sfp1 protein dra- matically decreased within 2h after shifting to the restrictive temperature (Fig. 2A), ------- COMMENT: 5e8e362b7dec8c54 43 gdlGN3cwt55aZvZ9pV8po6bQhnE In the tor2-287 and tor2-13 mutants, the Sfp1 protein dra- matically decreased within 2h after shifting to the restrictive temperature (Fig. 2A), ------- COMMENT: 5e8e362b7dec8c54 45 KGsZ6wQj6kvAKewOFmNqh9tnQ9k (SHOULD I CHANGE THIS TO DELAYED?????) The reduction of the Sfp1 protein upon nitrogen starvation was significantly delayed in the mts3-1 mutant (Fig. 2K), suggesting that the stability of Sfp1 during starvation is regulated by the proteasome. ------- COMMENT: 5e8e362b7dec8c54 46 2nFQ0OIljI0jsTV7TRJ5vcKp7i8 An in vitro kinase assay using Sfp1 as a substrate indicated that Sfp1 is a substrate of TORC1 (Fig. 2M). ------- COMMENT: 5e8e362b7dec8c54 47 KxM2abw+83bxntoYFQ+sh9SZmKA Our RNA-seq data indi- cated that among 141 RP and 292 Ribi genes in fission yeast (Supplementary material, Table S1), 31.9% of the RP genes (45 genes) and 26.4% of the Ribi genes (77 genes) are downregulated in the Dsfp1 mutant (Fig. 3B and C, and Supplementary material, Table S2). Collectively, these results imply that the Sfp1 transcription factor promotes the expression of the genes required for ribosome biosynthesis, a critical process for pro- tein synthesis linked to cell growth. ------- COMMENT: 5e8e362b7dec8c54 48 g5m/ANOQ6TD5GTyWSBysNzyPxw8 Importantly, no significant additive phenotype was observed when the mutations were combined (Fig. 4B), suggesting that Sfp1, Ifh1, and Fhl1 function in the same pathway that regulates cell proliferation. Consistently, like the Dsfp1 mutation (Fig. 1I), the Difh1 and Dfhl1 mutations were also able to ameliorate the growth defect caused by the absence of the functional GATOR1 complex (Fig. 4C). ------- COMMENT: 5e8e362b7dec8c54 49 dvk6lVy8GLS/UYEkEP/0UuT0/m4 Although the growth defect of the Dsfp1 mutant was observed on glucose medium, it grew normally on medium containing glycerol as the carbon source (Fig. 1H), similar to the situation in budding yeast.27. ALSO figure 4B for severity ------- COMMENT: 5e8e362b7dec8c54 50 HzYIqxL4E02OnjwlcTakuNB1XoA Notably, the absence of Sfp1 modestly alleviates the growth defect of the mutants lacking functional GATOR1 (Fig. 1I); the absence of GATOR1 causes a severe growth defect due to deregulated TORC1 activation,28 and therefore, the observed genetic interaction corroborates a functional link between Sfp1 and TORC1. ------- COMMENT: 5e8e362b7dec8c54 51 HzYIqxL4E02OnjwlcTakuNB1XoA Notably, the absence of Sfp1 modestly alleviates the growth defect of the mutants lacking functional GATOR1 (Fig. 1I); the absence of GATOR1 causes a severe growth defect due to deregulated TORC1 activation,28 and therefore, the observed genetic interaction corroborates a functional link between Sfp1 and TORC1. ------- COMMENT: 5e8e362b7dec8c54 52 HzYIqxL4E02OnjwlcTakuNB1XoA Notably, the absence of Sfp1 modestly alleviates the growth defect of the mutants lacking functional GATOR1 (Fig. 1I); the absence of GATOR1 causes a severe growth defect due to deregulated TORC1 activation,28 and therefore, the observed genetic interaction corroborates a functional link between Sfp1 and TORC1. ------- COMMENT: 5e8e362b7dec8c54 53 HzYIqxL4E02OnjwlcTakuNB1XoA Notably, the absence of Sfp1 modestly alleviates the growth defect of the mutants lacking functional GATOR1 (Fig. 1I); the absence of GATOR1 causes a severe growth defect due to deregulated TORC1 activation,28 and therefore, the observed genetic interaction corroborates a functional link between Sfp1 and TORC1. ------- COMMENT: 5e8e362b7dec8c54 54 5FgCgvm9FGTeBJ9PplAqhtIaX6U The interaction between Fhl1 and Ifh1 was impaired in the mutant expressing Fhl1 without the N-terminal 150 amino acid residues (Fig. 4E), indicating that the Ifh1-FHl1 inter- action depends on the forkhead-associated (FHA) domain of Fhl1. ------- COMMENT: 5e8e362b7dec8c54 55 nka9eM0QkLDtQRF1khz5yHxRb3o The Ifh1-Fhl1 associ- ation was detected both in the presence and absence of Sfp1, suggesting that Sfp1 is not required for their interaction (Fig. 4F). ------- COMMENT: 5e8e362b7dec8c54 56 JtSTEIYvzENuXH2CfBaYX7BEdAs It was found, however, that the amount of the Ifh1-Fhl1 complex was reduced in the Dsfp1 mutant when compared to that in the wild-type strain. ------- COMMENT: 5e8e362b7dec8c54 60 /4oxbGi0ak0lziAbrSHSeXARCt0 When TORC1 was inactivated in the tor2-13 or tor2-287 mutants at the restrictive temperature, the electrophoretic mobility of Ifh1 was notably decreased (Fig. 6A). The slow migrating form of Ifh1 was also observed when TORC1 was inactivated in wild-type cells starved of nitrogen (Fig. 6B). Phosphatase treatment experiments confirmed that slow-migrating Ifh1 was its phos- phorylated form (Fig. 6B). ------- COMMENT: 5e8e362b7dec8c54 61 /4oxbGi0ak0lziAbrSHSeXARCt0 When TORC1 was inactivated in the tor2-13 or tor2-287 mutants at the restrictive temperature, the electrophoretic mobility of Ifh1 was notably decreased (Fig. 6A). The slow migrating form of Ifh1 was also observed when TORC1 was inactivated in wild-type cells starved of nitrogen (Fig. 6B). Phosphatase treatment experiments confirmed that slow-migrating Ifh1 was its phos- phorylated form (Fig. 6B). ------- COMMENT: 5e8e362b7dec8c54 62 Rl1ZEjb4TYrCqkFwP5zZsbkl40M Remarkably, we discovered that Ifh1 accumulates in the nucleus in a TORC1- dependent manner. In wild-type cells expressing Ifh1 with the fluorescent mEGFP tag, Ifh1 appeared to be concentrated in the nucleus, with some cytoplasmic signals (Fig. 6D). ------- COMMENT: 5e8e362b7dec8c54 63 GBR6hCHkm3rwCHlC3g5x5AwlbYo or the tor2-287 mutation (Fig. 6E), the nuclear accumulation of Ifh1 was largely lost while no change in the Ifh1 protein levels was observed (Fig. 6B), implying that TORC1 promotes the nuclear localization of Ifh ------- COMMENT: 5e8e362b7dec8c54 64 sp7SA4Q7uUFLrzK7AonRGxHdP3k Moreover, the nuclear signal of Ifh1 was also significantly reduced in the Dsfp1 mutant (Fig. 6F), though neither the protein level nor the mobility shift of Ifh1 was affected by the Dsfp1 mutation (Fig. 6G). ------- COMMENT: 5e8e362b7dec8c54 65 03q8wt/d29y/688ArQBB6jddeWo Fhl1 was constitutively found in the nucleus, without being affected by TORC1 inactivation (Fig. 6D) or the loss of Sfp1 (Fig. 6F). ------- COMMENT: 5e8e362b7dec8c54 66 Cx6Vz9l3UZGnIk0KIYrnkYfoDB0 ------- COMMENT: 5e98db8475cc3f3a 1 9ZqRG6V5zHbuonTbMejRxrAWPMs Fig 1B (comment: there is a small G1 peak which get slightly bigger but they also cells arrested in G1 by -N, when refed and shifted to the restrictive temp cannot enter S phase but this is data not shown) ------- COMMENT: 5e98db8475cc3f3a 2 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: 5e98db8475cc3f3a 3 q6oErR5cXoXdItHwunJVLn1pEsE Fig 1B (comment: G1) ------- COMMENT: 5e98db8475cc3f3a 4 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 5e98db8475cc3f3a 5 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 5e98db8475cc3f3a 6 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 5e98db8475cc3f3a 7 IEXOkiUWzOm9cj/LI9e0Pz8MkSI Fig2B ------- COMMENT: 5e98db8475cc3f3a 8 IEXOkiUWzOm9cj/LI9e0Pz8MkSI Fig2B ------- COMMENT: 5e98db8475cc3f3a 10 IEXOkiUWzOm9cj/LI9e0Pz8MkSI Fig2B ------- COMMENT: 5e98db8475cc3f3a 11 H+Il45fAy44Svz+ppLE6jid47K0 Fig2C ------- COMMENT: 5e98db8475cc3f3a 12 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 5e98db8475cc3f3a 13 IEXOkiUWzOm9cj/LI9e0Pz8MkSI Fig2B ------- COMMENT: 5e98db8475cc3f3a 14 Yq6JbcrIyOvMby9op4YC4p/lXNI Fig2D (comment: the semi permissive temperature 34.5C for ded1-D5 allows it to suppress cdc19-P1) ------- COMMENT: 5e98db8475cc3f3a 15 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 5e98db8475cc3f3a 16 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 5e98db8475cc3f3a 17 psJbSkNdsDHlhumDf18+Zf6KWvI Fig3B ------- COMMENT: 5e98db8475cc3f3a 18 psJbSkNdsDHlhumDf18+Zf6KWvI Fig3B ------- COMMENT: 5e98db8475cc3f3a 20 O5ChQshWo9SMl9ef8VwaxdF3zLk Fig4B ------- COMMENT: 5e98db8475cc3f3a 21 c58h5dr8d9fnVPG2HW/yK9M9AAI Fig 5A (comment: 35S) ------- COMMENT: 5e98db8475cc3f3a 22 GJ8ndNR/lTcd0SgXCBi2umprr7Y Fig 5B (comment: 35S) ------- COMMENT: 5e98db8475cc3f3a 23 GJ8ndNR/lTcd0SgXCBi2umprr7Y Fig 5B (comment: 35S) ------- COMMENT: 5e98db8475cc3f3a 24 gOHNH0sZeuFMCUEJAyFg53SD+Yw Fig6 (comment: total protein translation not affected) ------- COMMENT: 5e98db8475cc3f3a 25 gOHNH0sZeuFMCUEJAyFg53SD+Yw Fig6 (comment: total protein translation not affected) ------- COMMENT: 5e98db8475cc3f3a 26 YUlHWUHQAhXkCbRr2JGy22Jhdww Fig7C, D (comment: The protein and mRNA levels are compared to cDNA-I which is also expressed from medium strength nmt1 promoter ON) ------- COMMENT: 5e98db8475cc3f3a 27 0EC+jcOdQo4q50bCLPfui5sy/dk Fig7 B ------- COMMENT: 5e98db8475cc3f3a 28 n1q/uh+Gy88dLHNOnkMoc8LTVOA Fig7 B (comment: over expression of cig2+ cDNAII suppresses the rescue of cdc21-M68 by ded1-1D5) ------- COMMENT: 5e98db8475cc3f3a 29 gHbgDGTd7crdZxs90Jx6mVL13zY Fig7 B (comment: over expression of cig2+ cDNAI partially suppresses the rescue of cdc21-M68 by ded1-1D5) ------- COMMENT: 5e98db8475cc3f3a 34 0dzd/YzPCjWEC89MeR5NbCYWqxE Fig9 ------- COMMENT: 5e98db8475cc3f3a 36 yopXCr8qEO+1Ut7h//blMmdU+Rw Fig10A ------- COMMENT: 5e98db8475cc3f3a 37 KPpSoxLfGhS4hdf87FrsFl9W8k0 Fig10B ------- COMMENT: 5e98db8475cc3f3a 39 0dzd/YzPCjWEC89MeR5NbCYWqxE Fig9 ------- COMMENT: 5ec20d07d1c694d3 3 60f1yPx08+XPh5xWaBinJTUGbtY Figures 5A and 5B ------- COMMENT: 5ec20d07d1c694d3 8 DadqlNdFwBVy2tQgQ2txbzlcLq8 fig 2 D ------- COMMENT: 5ec20d07d1c694d3 9 iyMraj5qmLEJtewo/fSEFxmllqQ figure 2D ------- COMMENT: 5ec20d07d1c694d3 10 iyMraj5qmLEJtewo/fSEFxmllqQ figure 2D ------- COMMENT: 5ec20d07d1c694d3 11 iyMraj5qmLEJtewo/fSEFxmllqQ figure 2D ------- COMMENT: 5ec20d07d1c694d3 12 z5HMI5xAnZm0/EpQ0PRsAAlO0yY figure3 (comment: incompatible with rap1 binding) ------- COMMENT: 5ec20d07d1c694d3 13 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 5ec20d07d1c694d3 14 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 5ec20d07d1c694d3 15 nPeBJP8Vt4uaHtOIwjnJ1E9mjok (comment: incompatible with DNA binding) ------- COMMENT: 5ec20d07d1c694d3 16 df2qjU0CXmaUtS00tRJ94RGzwV8 fig S5B ------- COMMENT: 5ec20d07d1c694d3 17 df2qjU0CXmaUtS00tRJ94RGzwV8 fig S5B ------- COMMENT: 5ec20d07d1c694d3 18 NT+joaEo+UiJlftdDnWlMiHfHeo fig S5B ------- COMMENT: 5ec20d07d1c694d3 19 df2qjU0CXmaUtS00tRJ94RGzwV8 fig S5B ------- COMMENT: 5ec20d07d1c694d3 20 df2qjU0CXmaUtS00tRJ94RGzwV8 fig S5B ------- COMMENT: 5ec20d07d1c694d3 21 df2qjU0CXmaUtS00tRJ94RGzwV8 fig S5B ------- COMMENT: 5ec20d07d1c694d3 22 60f1yPx08+XPh5xWaBinJTUGbtY Figures 5A and 5B ------- COMMENT: 5ec20d07d1c694d3 23 60f1yPx08+XPh5xWaBinJTUGbtY Figures 5A and 5B ------- COMMENT: 5ec20d07d1c694d3 24 6T7XfPK9lSh4As6/T9uPAt3roMA Figures 5C ------- COMMENT: 5ec20d07d1c694d3 25 6T7XfPK9lSh4As6/T9uPAt3roMA Figures 5C ------- COMMENT: 5ec20d07d1c694d3 27 LOKAIoue5CF9ou9WFtFOilP5VqQ (comment: transeferred from Junkos session PMID:30462301) ------- COMMENT: 5ec20d07d1c694d3 28 LOKAIoue5CF9ou9WFtFOilP5VqQ ((comment: transeferred from Junkos session PMID:30462301) ------- COMMENT: 5ec20d07d1c694d3 29 LOKAIoue5CF9ou9WFtFOilP5VqQ ((comment: transeferred from Junkos session PMID:30462301) ------- COMMENT: 5ec20d07d1c694d3 30 LOKAIoue5CF9ou9WFtFOilP5VqQ ((comment: transeferred from Junkos session PMID:30462301) ------- COMMENT: 5ecaf4c5a3d33afa 2 m6bsJ9Al6DLv1Xpl2G6BrsR1Mr8 Deletion of nrm1 or yox1 resulted in a $5–6-fold increase in cnp1 mRNA levels, whereas res2 deletion led to a more modest twofold increase (Figure 3A). ------- COMMENT: 5ecaf4c5a3d33afa 4 iB677Cp7vATNybd99fnQQ46o7dg Through the visual screen, we identified that three mutants, nrm1D, yox1D, and res2D, showed abnormal CENP-A distribution patterns (Figure 2A). In these mutants, Cnp1-GFP at centromeres tends to be brighter and also gives a high nucleoplasmic sig- nal (Figure 2A), suggesting an increase in Cnp1-GFP levels. ------- COMMENT: 5ecaf4c5a3d33afa 5 G404hhT1ZfYvRDjqhJRy0zUS7Jc Using DAPI staining, we observed that the MBF repressor mutant cells displayed lagging chromo- somes, chromosome bridges, and also unequal nuclei segre- gation during mitosis (Figure 2C and Figure S4). ------- COMMENT: 5ecaf4c5a3d33afa 7 iB677Cp7vATNybd99fnQQ46o7dg Through the visual screen, we identified that three mutants, nrm1D, yox1D, and res2D, showed abnormal CENP-A distribution patterns (Figure 2A). In these mutants, Cnp1-GFP at centromeres tends to be brighter and also gives a high nucleoplasmic sig- nal (Figure 2A), suggesting an increase in Cnp1-GFP levels. ------- COMMENT: 5ecaf4c5a3d33afa 8 G404hhT1ZfYvRDjqhJRy0zUS7Jc Using DAPI staining, we observed that the MBF repressor mutant cells displayed lagging chromo- somes, chromosome bridges, and also unequal nuclei segre- gation during mitosis (Figure 2C and Figure S4). ------- COMMENT: 5ecaf4c5a3d33afa 10 iB677Cp7vATNybd99fnQQ46o7dg Through the visual screen, we identified that three mutants, nrm1D, yox1D, and res2D, showed abnormal CENP-A distribution patterns (Figure 2A). In these mutants, Cnp1-GFP at centromeres tends to be brighter and also gives a high nucleoplasmic sig- nal (Figure 2A), suggesting an increase in Cnp1-GFP levels. ------- COMMENT: 5ecaf4c5a3d33afa 11 G404hhT1ZfYvRDjqhJRy0zUS7Jc Using DAPI staining, we observed that the MBF repressor mutant cells displayed lagging chromo- somes, chromosome bridges, and also unequal nuclei segre- gation during mitosis (Figure 2C and Figure S4). ------- COMMENT: 5ecaf4c5a3d33afa 12 f8HQggk4y9Njgt/U3u+zIwaQ7gc Accordingly, the peak of septation matches with the peak of expression of the histone H3 (hht1 gene) as histones are highly expressed during S phase (Figure 1A). ------- COMMENT: 5ecaf4c5a3d33afa 13 HLYP+5ky5f4kRxkWWDh91TVGCL8 Consistent with the previous report (Takahashi et al. 2000), cnp1 mRNA peaks before the transcription of histone H3, $120 min after elutriation, showing an approximately twofold increase. This pattern of expression coincides with the expression of cdc18, a well-known cell cycle-regulated gene expressed specifically in G1 phase (Figure 1A). ------- COMMENT: 5ecaf4c5a3d33afa 14 HLYP+5ky5f4kRxkWWDh91TVGCL8 Consistent with the previous report (Takahashi et al. 2000), cnp1 mRNA peaks before the transcription of histone H3, $120 min after elutriation, showing an approximately twofold increase. This pattern of expression coincides with the expression of cdc18, a well-known cell cycle-regulated gene expressed specifically in G1 phase (Figure 1A). ------- COMMENT: 5ecaf4c5a3d33afa 15 RPZd8J/WvQkwhE2kYtvrdTr8V8U Cnp1-GFP protein increases ap- proximately twofold at 165-min postelutriation. This resulindicates that the mRNA increase in G1 results in augmenta- tion of the Cnp1 protein level in S phase. ------- COMMENT: 5ecaf4c5a3d33afa 22 iB677Cp7vATNybd99fnQQ46o7dg Through the visual screen, we identified that three mutants, nrm1D, yox1D, and res2D, showed abnormal CENP-A distribution patterns (Figure 2A). In these mutants, Cnp1-GFP at centromeres tends to be brighter and also gives a high nucleoplasmic sig- nal (Figure 2A), suggesting an increase in Cnp1-GFP levels. ------- COMMENT: 5ecaf4c5a3d33afa 23 iB677Cp7vATNybd99fnQQ46o7dg Through the visual screen, we identified that three mutants, nrm1D, yox1D, and res2D, showed abnormal CENP-A distribution patterns (Figure 2A). In these mutants, Cnp1-GFP at centromeres tends to be brighter and also gives a high nucleoplasmic sig- nal (Figure 2A), suggesting an increase in Cnp1-GFP levels. ------- COMMENT: 5ecaf4c5a3d33afa 24 iB677Cp7vATNybd99fnQQ46o7dg Through the visual screen, we identified that three mutants, nrm1D, yox1D, and res2D, showed abnormal CENP-A distribution patterns (Figure 2A). In these mutants, Cnp1-GFP at centromeres tends to be brighter and also gives a high nucleoplasmic sig- nal (Figure 2A), suggesting an increase in Cnp1-GFP levels. ------- COMMENT: 5ecaf4c5a3d33afa 26 G404hhT1ZfYvRDjqhJRy0zUS7Jc Using DAPI staining, we observed that the MBF repressor mutant cells displayed lagging chromo- somes, chromosome bridges, and also unequal nuclei segre- gation during mitosis (Figure 2C and Figure S4). ------- COMMENT: 5ecaf4c5a3d33afa 27 G404hhT1ZfYvRDjqhJRy0zUS7Jc Using DAPI staining, we observed that the MBF repressor mutant cells displayed lagging chromo- somes, chromosome bridges, and also unequal nuclei segre- gation during mitosis (Figure 2C and Figure S4). ------- COMMENT: 5ecaf4c5a3d33afa 28 G404hhT1ZfYvRDjqhJRy0zUS7Jc Using DAPI staining, we observed that the MBF repressor mutant cells displayed lagging chromo- somes, chromosome bridges, and also unequal nuclei segre- gation during mitosis (Figure 2C and Figure S4). ------- COMMENT: 5ecaf4c5a3d33afa 29 G404hhT1ZfYvRDjqhJRy0zUS7Jc Using DAPI staining, we observed that the MBF repressor mutant cells displayed lagging chromo- somes, chromosome bridges, and also unequal nuclei segre- gation during mitosis (Figure 2C and Figure S4). ------- COMMENT: 5ecaf4c5a3d33afa 30 G404hhT1ZfYvRDjqhJRy0zUS7Jc Using DAPI staining, we observed that the MBF repressor mutant cells displayed lagging chromo- somes, chromosome bridges, and also unequal nuclei segre- gation during mitosis (Figure 2C and Figure S4). ------- COMMENT: 5ecaf4c5a3d33afa 31 G404hhT1ZfYvRDjqhJRy0zUS7Jc Using DAPI staining, we observed that the MBF repressor mutant cells displayed lagging chromo- somes, chromosome bridges, and also unequal nuclei segre- gation during mitosis (Figure 2C and Figure S4). ------- COMMENT: 5ecaf4c5a3d33afa 32 G404hhT1ZfYvRDjqhJRy0zUS7Jc Using DAPI staining, we observed that the MBF repressor mutant cells displayed lagging chromo- somes, chromosome bridges, and also unequal nuclei segre- gation during mitosis (Figure 2C and Figure S4). ------- COMMENT: 5ecaf4c5a3d33afa 33 m6bsJ9Al6DLv1Xpl2G6BrsR1Mr8 Deletion of nrm1 or yox1 resulted in a $5–6-fold increase in cnp1 mRNA levels, whereas res2 deletion led to a more modest twofold increase (Figure 3A). ------- COMMENT: 5ecaf4c5a3d33afa 34 tB/KdtfmZTKNEFbnjEZ7K5vJ0aM ChIP analysis showed that Nrm1, Yox1, and Res2 bind to the cnp1 promoter (Figure 4B). ------- COMMENT: 5ecaf4c5a3d33afa 35 tB/KdtfmZTKNEFbnjEZ7K5vJ0aM ChIP analysis showed that Nrm1, Yox1, and Res2 bind to the cnp1 promoter (Figure 4B). ------- COMMENT: 5ecaf4c5a3d33afa 36 tB/KdtfmZTKNEFbnjEZ7K5vJ0aM ChIP analysis showed that Nrm1, Yox1, and Res2 bind to the cnp1 promoter (Figure 4B). ------- COMMENT: 5ef3337d730491a3 3 2WDCovXteXkTX4MscdFTi1sfZJM Fig 2C, cdc10 dependent transcription occurs during mitotic exit ------- COMMENT: 5ef3337d730491a3 8 ZGdcxhmm7MPbCm514pMYUjSyOJo Fig3A cdc10 is a cdc18 transcriptional regulator see Fig2C ------- COMMENT: 5ef3337d730491a3 11 vvX8WEjt8Pc7o6BGc1cqR7zdejs Fig3B cdc10 is a cdc18 transcriptional regulator see Fig2C ------- COMMENT: 5ef3337d730491a3 12 psJbSkNdsDHlhumDf18+Zf6KWvI Fig3B ------- COMMENT: 5ef3337d730491a3 15 AjrhFIdQST/r4Rvnl8ru+jlXJj0 Fig4C cd18 N term deletion can accumulate in a metaphase arrest ------- COMMENT: 5ef3337d730491a3 17 40GJ3TXOVLw6u6vMlERJo7puVcU Fig4E cdc18 lacking cdc2 phosphorylation sites accumulates immediately as cells progress into mitosis ------- COMMENT: 5ef3337d730491a3 23 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: 5ef3337d730491a3 26 REqA86wgEckE1+po1IZQ8wC5RUg Fig5C ------- COMMENT: 5ef3337d730491a3 27 B5kuU22A8v2CBb2zeFG0PcS7nzw Fig5D ------- COMMENT: 5ef3337d730491a3 29 AjrhFIdQST/r4Rvnl8ru+jlXJj0 Fig4C (comment: cd18 N term deletion can accumulate in a metaphase arrest) ------- COMMENT: 5f0343986c82c553 5 eUgVAvBgFd4SPtZff3IA6MtU8+c Skb1 and Slf1 (SPAC821.03C) mutually depend to form node-like structures on the plasma membrane. ------- COMMENT: 5f0343986c82c553 6 eUgVAvBgFd4SPtZff3IA6MtU8+c Skb1 and Slf1 (SPAC821.03C) mutually depend to form node-like structures on the plasma membrane. ------- COMMENT: 5f656dfe4953442a 1 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 5f656dfe4953442a 2 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 5f656dfe4953442a 3 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 5f656dfe4953442a 4 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 5f656dfe4953442a 5 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 5f656dfe4953442a 6 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 5f656dfe4953442a 7 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 5f656dfe4953442a 8 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 5f656dfe4953442a 9 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 5f656dfe4953442a 10 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 5f656dfe4953442a 11 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 5f656dfe4953442a 12 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 5f656dfe4953442a 13 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 5f656dfe4953442a 14 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 5f656dfe4953442a 15 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 5f656dfe4953442a 16 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 5f656dfe4953442a 17 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 5f656dfe4953442a 18 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 5f656dfe4953442a 19 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 5f656dfe4953442a 20 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 5f656dfe4953442a 21 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 5f656dfe4953442a 22 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 5f656dfe4953442a 23 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 5f656dfe4953442a 24 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 5f656dfe4953442a 25 fNUKK04nVCSkC90/wOdBdI475q8 Fig. 2B and D ------- COMMENT: 5f656dfe4953442a 26 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 5f656dfe4953442a 27 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 5f656dfe4953442a 28 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 5f656dfe4953442a 29 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 5f656dfe4953442a 30 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 5f656dfe4953442a 31 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 5f656dfe4953442a 32 MpML2E+8wq5nwC1YfM2LDELzzPU Fig. 2E ------- COMMENT: 5f656dfe4953442a 33 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 5f656dfe4953442a 34 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 5f656dfe4953442a 35 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 5f656dfe4953442a 36 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 5f656dfe4953442a 37 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 5f656dfe4953442a 38 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 5f656dfe4953442a 39 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 5f656dfe4953442a 40 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 5f656dfe4953442a 41 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 5f656dfe4953442a 42 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 5f656dfe4953442a 43 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 5f656dfe4953442a 44 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 5f656dfe4953442a 45 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 5f656dfe4953442a 46 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 5f656dfe4953442a 47 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 5f656dfe4953442a 48 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 5f656dfe4953442a 49 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 5f656dfe4953442a 50 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 5f656dfe4953442a 51 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 5f656dfe4953442a 52 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 5f656dfe4953442a 53 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 5f656dfe4953442a 54 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 5f656dfe4953442a 55 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 5f656dfe4953442a 56 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 5f656dfe4953442a 57 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: 5f656dfe4953442a 58 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: 5f656dfe4953442a 59 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: 5f656dfe4953442a 60 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 5f656dfe4953442a 61 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 5f656dfe4953442a 62 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 5f656dfe4953442a 63 mKyZ65iOvmrq5ZTSq6F9f9EJzUw Our analyses suggest that Rik1 is a crucial factor involved in RNAi- mediated targeting of ClrC to heterochromatic repeat elements. ------- COMMENT: 5f656dfe4953442a 64 Tdfc5mtYa5S07LgRaYkVAUezUl8 The results presented here show that binding of Clr4 to H3K9me via its chromodomain is crucial for the spreading of heterochromatic structures. ------- COMMENT: 5f656dfe4953442a 65 Tdfc5mtYa5S07LgRaYkVAUezUl8 The results presented here show that binding of Clr4 to H3K9me via its chromodomain is crucial for the spreading of heterochromatic structures. ------- COMMENT: 5f656dfe4953442a 66 Tdfc5mtYa5S07LgRaYkVAUezUl8 The results presented here show that binding of Clr4 to H3K9me via its chromodomain is crucial for the spreading of heterochromatic structures. ------- COMMENT: 5f8aff66a56b4439 1 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 5f8aff66a56b4439 2 V319/1NfjNjkvxZMZTidAlzNugQ Figure 1C ------- COMMENT: 5f8aff66a56b4439 3 iAcbpl1ew+nFwb8AiAqOLZxpvN8 Figure 1D ------- COMMENT: 5f8aff66a56b4439 4 iAcbpl1ew+nFwb8AiAqOLZxpvN8 Figure 1D ------- COMMENT: 5f8aff66a56b4439 5 iAcbpl1ew+nFwb8AiAqOLZxpvN8 Figure 1D ------- COMMENT: 5f8aff66a56b4439 6 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 5f8aff66a56b4439 7 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 5f8aff66a56b4439 8 iAcbpl1ew+nFwb8AiAqOLZxpvN8 Figure 1D ------- COMMENT: 5f8aff66a56b4439 9 iAcbpl1ew+nFwb8AiAqOLZxpvN8 Figure 1D ------- COMMENT: 5f8aff66a56b4439 10 iAcbpl1ew+nFwb8AiAqOLZxpvN8 Figure 1D ------- COMMENT: 5f8aff66a56b4439 11 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 5f8aff66a56b4439 12 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 5f8aff66a56b4439 16 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 5f8aff66a56b4439 17 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 5f8aff66a56b4439 18 +8KhfBKi9uXxtWkbxuUUiB6UXrs Figure 1F ------- COMMENT: 5f8aff66a56b4439 19 nUCcEDoowWdBc8w5z3A4wbzAAHc Figure 1F ------- COMMENT: 5f8aff66a56b4439 20 aOqYL7F2KV5d9E4mVC2uoiGmluk Figure 1E ------- COMMENT: 5f8aff66a56b4439 21 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: 5f8aff66a56b4439 22 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: 5f8aff66a56b4439 23 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: 5f8aff66a56b4439 24 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: 5f8aff66a56b4439 27 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: 5f8aff66a56b4439 46 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 5f8aff66a56b4439 57 Yz3w4ufxRfIpoZ+TYY1t7+8IB5I ------- COMMENT: 5f8aff66a56b4439 113 CtTnGhCjBW/ODzCIB2oZhJQ3/NI (comment: CHECK asp1-H397A) ------- COMMENT: 5f8aff66a56b4439 118 0iNep8FuMVCUDtY1ZSP2dE75hLs Octo phosphatase IP8 is a relevant substrate for the Aps1 pyrophos- phatase with respect to phosphate homeostasis. ------- COMMENT: 5f8aff66a56b4439 122 XzdUtwSCNJ9fnjKYG1heHOwtSwo lncRNA. Specifically, it is hypothesized that loss of the Ser7-PO4 or Ser5-PO4 marks leads to precocious termination of prt lncRNA transcription prior to the pho1 promoter and loss of the Thr4-PO4 mark reduces termination and hence increases transcription across the pho1 promoter (8) (Figure 1A). ------- COMMENT: 5f8aff66a56b4439 123 wVNTP6AaqxSgNCOZVCbPanrrbxA (comment: CHECK target genes pho1, pho84, and tgp1) ------- COMMENT: 5f8aff66a56b4439 124 Puop3muJNyB6+GCb4LMfbHxz/ZA (comment: CHECK target genes repressing lncRNA) ------- COMMENT: 5fb962029c56cdd6 23 lJahfHprChuhVBnvHTpJy39yt2Q These data indicate that high affinity binding sites for the Lsm1–7 complex must be at the 3′ termini of RNA. ------- COMMENT: 5fb962029c56cdd6 24 i1GRrDSXCqkuon6UQxI9bBc3mxY We conclude that the carboxy-terminal 12 amino acids of Lsm1 are important for the binding specificity of Lsm1–7. ------- COMMENT: 5fb962029c56cdd6 25 KaMoTtJpIIIwHlsjSt0M77NeERo Lsm1Δ56C–7 can only bind tightly to the RNA with a 5′ stem–loop and single stranded 3′ end (Kd = 70 and 32 nM, respectively) (Fig. 5C). ------- COMMENT: 5fe213e9b923b10e 16 1jchpUsBS8G8KqhhZfMZHMJWpHs punctate in wild type, diffuse throughout nucleus in mutant ------- COMMENT: 5fe4a0c49baf997f 1 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 5fe4a0c49baf997f 2 p23VaE2MmzhdxFHAPnFMA17hdOk Fig. 2A and B ------- COMMENT: 5fe4a0c49baf997f 3 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 5fe4a0c49baf997f 4 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 5fe4a0c49baf997f 5 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 5fe4a0c49baf997f 6 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 5fe4a0c49baf997f 7 5atobt4486TmnYYXvYjmryjGb28 Several Mc peaks correspond to WTF (With Tf) elements on chromosome 3 and solo LTRs dispersed across the genome (Fig. 3). ------- COMMENT: 5fe4a0c49baf997f 8 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 5fe4a0c49baf997f 9 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 5fe4a0c49baf997f 10 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 5fe4a0c49baf997f 11 bP92yyYSWtMHsR7yOPds1iL/4mI Abp1 is localized at the swi2 promoter (Fig. 6A), in addition to its localization at LTRs. ------- COMMENT: 5fe4a0c49baf997f 12 bP92yyYSWtMHsR7yOPds1iL/4mI Abp1 is localized at the swi2 promoter (Fig. 6A), in addition to its localization at LTRs. ------- COMMENT: 5fe4a0c49baf997f 13 GiU9OuJkzQhTGzbi+6lSBIDTOUI Fig. 6A and B ------- COMMENT: 5fe4a0c49baf997f 14 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 5fe4a0c49baf997f 15 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 5fe4a0c49baf997f 16 hX27p0CZR6/iq9qp0MQAYq/vGE0 Abp1 bound to the swi2 pro- moter region facilitates preferential targeting of Mc, which normally binds an M-box sequence motif to activate M-specific genes ------- COMMENT: 5fec0b80ee107b83 1 GXj0wzZSYyyqsitpqQq+fowRzhA In dma1Δ cells, a single slower migrating form of Sid4 was detected, which was collapsed by phosphatase treatment, indicating that Sid4 is phosphorylated in vivo (Figure 1A, lanes 3 and 4). In vivo radiolabeling experiments validated Sid4 as a phospho-protein and revealed that Sid4 is phosphorylated on serines and threonines (Figure S1A–C). ------- COMMENT: 5fec0b80ee107b83 2 j4fYKMQpRwhMJVFu76Kw1c7bOnk Although mutating S278 to alanine abolished Sid4 ubiquitination (Figure 1D) ------- COMMENT: 5fec0b80ee107b83 3 8BVSqxo2BQNHuRnLMqdF7t7xWx4 mutating S278 to a glutamate did not affect Sid4 ubiquitination (Figure 1D). ------- COMMENT: 5fec0b80ee107b83 4 U+YT4nSela/ePJp09r+CVVVWHWQ Although Dma1-GFP still localized to SPBs in sid4(T275A) mutant cells (Figure S1F), ------- COMMENT: 5fec0b80ee107b83 5 U+YT4nSela/ePJp09r+CVVVWHWQ Although Dma1-GFP still localized to SPBs in sid4(T275A) mutant cells (Figure S1F), ------- COMMENT: 5fec0b80ee107b83 6 U+YT4nSela/ePJp09r+CVVVWHWQ Although Dma1-GFP still localized to SPBs in sid4(T275A) mutant cells (Figure S1F), ------- COMMENT: 5fec0b80ee107b83 7 7qZ16v0Yv6cxpG7ftuYUAUBiZ94 Thus, phosphorylation on both T275 and S278 is necessary and sufficient to support binding of the Dma1 FHA domain to Sid4 and Sid4 ubiquitination. ------- COMMENT: 5fec0b80ee107b83 9 8TWzicApyTxJBPjlH1wF+951Md4 cells bypassed the arrest after 5 hrs (Figure 2C). ......These data indicate that mutating T275 eliminates Dma1-dependent checkpoint signaling. ------- COMMENT: 5fec0b80ee107b83 10 XgrOiDqIaSsrJcG/pBzDQltfkZg cells bypassed the arrest after 5 hrs (Figure 2C). ------- COMMENT: 5fec0b80ee107b83 12 idpJ4olrUaUYtosUImUblvlM6iw In corroboration of these findings, sid4(T275A) mutants were refractory to dma1 overexpression lethality (Figure 2D). ------- COMMENT: 5fec0b80ee107b83 13 fNxnmQ/PS683hO1LBLzGazuVzOg dma1 overexpression rescued by sid4-T275A ------- COMMENT: 5fec0b80ee107b83 14 4pl4cD6WB1qmgcA8YVNwvrtBBdM In cells growing asynchronously, both Hhp1-GFP and Hhp2-GFP localized to the nucleus, SPBs, and the cell division site, although Hhp2-GFP was more prominent at the division site compared to Hhp1-GFP (Figure 3B). ------- COMMENT: 5fec0b80ee107b83 16 4pl4cD6WB1qmgcA8YVNwvrtBBdM In cells growing asynchronously, both Hhp1-GFP and Hhp2-GFP localized to the nucleus, SPBs, and the cell division site, although Hhp2-GFP was more prominent at the division site compared to Hhp1-GFP (Figure 3B). ------- COMMENT: 5fec0b80ee107b83 20 XQ53kaGypVrpjR5L4jeLy4NO6LY Hhp1-GFP localization at SPBs is Sid4 independent (Figure S3E) ------- COMMENT: 5fec0b80ee107b83 21 XQ53kaGypVrpjR5L4jeLy4NO6LY Hhp1-GFP localization at SPBs is Sid4 independent (Figure S3E) ------- COMMENT: 5fec0b80ee107b83 22 W3NRKvphKYs6JFrKfi5npVAkjY4 assayed with human CK1, process from phenotypes (dma1 dependent pathway) ------- COMMENT: 5fec0b80ee107b83 23 pJtoz9s9i/L00LdR62XGdp/hjKA assayed with human CK1 ------- COMMENT: 600ec028617b4964 4 Exs+WkapKZVyxTmaBktQn0knEQQ (comment: CHECK ABOLISHED) ------- COMMENT: 600ec028617b4964 5 Exs+WkapKZVyxTmaBktQn0knEQQ (comment: CHECK ABOLISHED) ------- COMMENT: 600ec028617b4964 6 Exs+WkapKZVyxTmaBktQn0knEQQ (comment: CHECK ABOLISHED ------- COMMENT: 60100461d789b19f 19 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 60100461d789b19f 20 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: 60793151324a1ef2 1 mucTVgCZg9IcLxsNMD3POHC4auQ Fig. 2A, Fig. S2A, Fig. 4A ------- COMMENT: 60793151324a1ef2 2 7x0nP4aOijHgGN7H1+EwbTQCIGE (comment: No more sensitive than rad57∆.) Fig. 2B, Fig. S2B, Fig. 4D ------- COMMENT: 60793151324a1ef2 3 5lNBUqufVr8gpGECQ983Xc1iNZg (comment: Similar sensitivity to sfr1∆ rad51∆) Fig. 2C, Fig. S2C, Fig. 4C ------- COMMENT: 60793151324a1ef2 4 a/Xmsz8749rFedBTVV9TZaEX920 Fig. 2E, Fig. 7A ------- COMMENT: 60793151324a1ef2 5 MpML2E+8wq5nwC1YfM2LDELzzPU Fig. 2E ------- COMMENT: 60793151324a1ef2 6 9R/a8tJVUfnN6hWqgOdOp8YJy0o Fig. 7A, Fig. 7B, Fig. S7-1B ------- COMMENT: 60793151324a1ef2 7 lt2pkx5XPTUurrYIIsqW8bTRKF8 Fig. 7C ------- COMMENT: 60793151324a1ef2 8 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 60793151324a1ef2 9 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 60793151324a1ef2 11 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 60793151324a1ef2 12 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 60793151324a1ef2 13 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 60793151324a1ef2 14 OavMzXS63qGcX/S3oBGcmbxH+Ag (comment: Similar sensitivity to rad51∆) Fig. S4D ------- COMMENT: 60793151324a1ef2 15 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 60793151324a1ef2 16 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 60793151324a1ef2 17 UGvkJoHws2Bc8fSlEeFX2eVNC94 (comment: Similar sensitivity to rad51∆) Fig. 4A ------- COMMENT: 60793151324a1ef2 18 VZ8eIEhAAXY23wa7WpsIHOQCqMQ (comment: Number of Rad51 foci similar to rad52∆ and rad57∆ sfr1∆) Fig. 5C ------- COMMENT: 60793151324a1ef2 19 ZSoIdAAkba/kthzeLNn7KxqZ/Bw Fig. S6B ------- COMMENT: 60793151324a1ef2 20 AoxCF6165PlYpVxgfR9odj3O31Y Fig. S6C ------- COMMENT: 60793151324a1ef2 21 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 60793151324a1ef2 22 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: 60793151324a1ef2 23 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 60793151324a1ef2 24 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 60793151324a1ef2 25 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: 60793151324a1ef2 26 MpML2E+8wq5nwC1YfM2LDELzzPU Fig. 2E ------- COMMENT: 609a96b3831b9b45 1 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: 609a96b3831b9b45 2 3BAy7rKC8GXVa2a2+YR6/VVMIMY Sl8-GFP colocalizes with nuclear periphery labeled by Cut11-mCherry (Fig. 4A) ------- COMMENT: 609a96b3831b9b45 3 JdAVL1TUH0cKOvP0czAzu6t+8fU Slx8-GFP colocalizes wih Mis6-RFP (Fig. 5) ------- COMMENT: 609a96b3831b9b45 4 kuRdbpnFrU9sjksgW9GQwEQ1424 (Fig. 5) ------- COMMENT: 609a96b3831b9b45 8 NE4wrlPdxQiECxoNvIfOQXp+yvo (Fig. 7B and C) ------- COMMENT: 609a96b3831b9b45 9 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: 609a96b3831b9b45 10 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: 609a96b3831b9b45 11 NE4wrlPdxQiECxoNvIfOQXp+yvo (Fig. 7B and C) ------- COMMENT: 609a96b3831b9b45 12 tdY+sTkcVXQXSK7tyg52iDlFg+Y (Fig. S1A) ------- COMMENT: 609a96b3831b9b45 13 tdY+sTkcVXQXSK7tyg52iDlFg+Y (Fig. S1A) ------- COMMENT: 609a96b3831b9b45 14 tdY+sTkcVXQXSK7tyg52iDlFg+Y (Fig. S1A) ------- COMMENT: 609a96b3831b9b45 15 E/4PM8gDFijtfHTJmcDpKMCM6zk Slx8-GFP colocalizes with Sid4-RFP (Fig. 5) ------- COMMENT: 609a96b3831b9b45 16 YXHGFHU5LadekPlbrwHvPt7VWDU (Fig. 4) ------- COMMENT: 609a96b3831b9b45 17 cgUZ9Rgpi1C57s7n5arqT9ZUWoE (Fig. 2B and C) ------- COMMENT: 609a96b3831b9b45 18 cgUZ9Rgpi1C57s7n5arqT9ZUWoE (Fig. 2B and C) ------- COMMENT: 609a96b3831b9b45 19 VgI/Msukwv4bOVlDmPXEMtQI3vY (Fig. 2B, C and D) ------- COMMENT: 609a96b3831b9b45 20 kuRdbpnFrU9sjksgW9GQwEQ1424 (Fig. 5) ------- COMMENT: 609a96b3831b9b45 21 khsisV/+a3EzkwQ6iLhnNm1HrpM (Fig. 6) ------- COMMENT: 609a96b3831b9b45 22 khsisV/+a3EzkwQ6iLhnNm1HrpM (Fig. 6) ------- COMMENT: 609a96b3831b9b45 23 NE4wrlPdxQiECxoNvIfOQXp+yvo (Fig. 7B and C) ------- COMMENT: 609a96b3831b9b45 24 NE4wrlPdxQiECxoNvIfOQXp+yvo (Fig. 7B and C) ------- COMMENT: 609a96b3831b9b45 25 NE4wrlPdxQiECxoNvIfOQXp+yvo (Fig. 7B and C) ------- COMMENT: 609a96b3831b9b45 26 khsisV/+a3EzkwQ6iLhnNm1HrpM (Fig. 6) ------- COMMENT: 60a204a00657319d 17 xPW3QG3QjzHrcrriNGHeQMgGmwM (comment: CHECK also increased (WT overexppression)) (comment: CHECK normal (WT)) ------- COMMENT: 60a2109be6c06e32 1 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 60a2109be6c06e32 2 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 60a2109be6c06e32 3 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 60a2109be6c06e32 4 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 60a2109be6c06e32 5 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 60a2109be6c06e32 6 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 60a2109be6c06e32 7 LX30Pzyp0dXIvIlY0jjx8zyNWPA Fig. 2F ------- COMMENT: 60a2109be6c06e32 8 fogVowcObkoWi79lNpy/RoRGREw Fig. 2G ------- COMMENT: 60a2109be6c06e32 9 fogVowcObkoWi79lNpy/RoRGREw Fig. 2G ------- COMMENT: 60a2109be6c06e32 10 fogVowcObkoWi79lNpy/RoRGREw Fig. 2G ------- COMMENT: 60a2109be6c06e32 11 fogVowcObkoWi79lNpy/RoRGREw Fig. 2G ------- COMMENT: 60a2109be6c06e32 12 fogVowcObkoWi79lNpy/RoRGREw Fig. 2G ------- COMMENT: 60a2109be6c06e32 13 fogVowcObkoWi79lNpy/RoRGREw Fig. 2G ------- COMMENT: 60a2109be6c06e32 14 fogVowcObkoWi79lNpy/RoRGREw Fig. 2G ------- COMMENT: 60a2109be6c06e32 15 fogVowcObkoWi79lNpy/RoRGREw Fig. 2G ------- COMMENT: 60a2109be6c06e32 16 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 60a2109be6c06e32 17 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 60a2109be6c06e32 18 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 60a2109be6c06e32 19 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 60a2109be6c06e32 20 INRfNgD265gDfQsQLz0NCDef3v8 Swi5 localization pattern closely resembled that of Swi2. Fig. 5 ------- COMMENT: 60a2109be6c06e32 21 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 60b7b54f3794075a 1 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 60b7b54f3794075a 2 LkGQQw8FQBPnHuF6zSR3Jquhnkc fig 1C ------- COMMENT: 60b7b54f3794075a 3 GSDoKVHrCsiUO6zYP0Vg8a8VAAQ fig 1D ------- COMMENT: 60b7b54f3794075a 4 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 60b7b54f3794075a 5 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 60b7b54f3794075a 6 e8/5kwIqjdteqRCP5PpAkij2QI0 fig 1C (comment: supressed by hexestrol) ------- COMMENT: 60b7b54f3794075a 7 EqyJkVkPm8qYFZ9zTJzgRZuSD64 fig 1D (comment: supressed by hexestrol or clomifene) ------- COMMENT: 60b7b54f3794075a 13 oLhQHQb+qlDox9+3ypMnOy4BrTY (comment: supressed dna fragmentation) ------- COMMENT: 60b7b54f3794075a 14 oLhQHQb+qlDox9+3ypMnOy4BrTY (comment: supressed dna fragmentation) ------- COMMENT: 60b7b54f3794075a 15 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 60b7b54f3794075a 16 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 60b7b54f3794075a 17 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 60cfb81b2cb37543 1 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 60cfb81b2cb37543 2 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 60cfb81b2cb37543 3 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 60cfb81b2cb37543 4 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 60cfb81b2cb37543 10 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 60eab3fe44de3034 2 wzwz1QfiJqb1Mkr7F66z515AbKg (comment: switches specificity from direct repeats to inverted repeats) ------- COMMENT: 60eea6892c654dff 1 V8aY9cMSuflWPDnZXK1Ej8D/EEk fig 1b ------- COMMENT: 60eea6892c654dff 2 THLTkbJvk554qMRP8h+KjMcEfnU fig 1b , fig 2 ------- COMMENT: 60eea6892c654dff 4 0lWCdYbsTv5tg5UMlHJ8fZ4ZVOg figure S1B–S1C ------- COMMENT: 60eea6892c654dff 5 0lWCdYbsTv5tg5UMlHJ8fZ4ZVOg figure S1B–S1C ------- COMMENT: 60eea6892c654dff 6 0lWCdYbsTv5tg5UMlHJ8fZ4ZVOg figure S1B–S1C ------- COMMENT: 60eea6892c654dff 9 WuNxHF9jqVwSvE5vxzKLbio5Pv8 Figure 1F–1G ------- COMMENT: 60eea6892c654dff 10 PGBi0PdCco11hpgSC1dv1P+JQrg Figure S1F ------- COMMENT: 60eea6892c654dff 11 PGBi0PdCco11hpgSC1dv1P+JQrg Figure S1F ------- COMMENT: 60eea6892c654dff 12 PGBi0PdCco11hpgSC1dv1P+JQrg Figure S1F ------- COMMENT: 60eea6892c654dff 16 yrA2g8uUvpHbEZiHi7k52HZa0mA T-shapes always arose in cells that the tea1D growth pattern dictated should grow at their new ends (Figure 2D–2E) but that actually grew at neither (Figure 2E and 2G) ------- COMMENT: 60eea6892c654dff 17 eIZ2xCICe3H5mJKZbLWGui20vss As was observed previously [28], cytoplasmic Fic1-GFP localizes to cell tips during interphase and later to the CR during cell division (Figure 3A). ------- COMMENT: 60eea6892c654dff 18 eIZ2xCICe3H5mJKZbLWGui20vss As was observed previously [28], cytoplasmic Fic1-GFP localizes to cell tips during interphase and later to the CR during cell division (Figure 3A). ------- COMMENT: 60eea6892c654dff 19 23A7gKYH++mwxpDaLPxFOcAYx6Y fig 3C ------- COMMENT: 60eea6892c654dff 20 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: 60eea6892c654dff 21 vteWAdG8N9a93kzVg0k7ME0QKk4 Figure 3D–3F ------- COMMENT: 60eea6892c654dff 22 C6u0bg8KEYIj6A823ggA1xP/FPc Figure 3G ------- COMMENT: 60eea6892c654dff 23 K5VaIcg7u0r4ld0aJzpfv7aP8D8 Figure 4A–4C and Figure S3A–S3B ------- COMMENT: 60eea6892c654dff 24 d5xS2hKEB2A35T11l3LLBbhTh04 Mutation of PxxPs 10 and 11 in combination, or P257 of PxxP 11 alone, abolished the two-hybrid interaction (Figure S3D), and the P257A mutation eliminated co-immunoprecipitation of Fic1- FLAG3 with Cdc15 in vivo (Figure 4D). ------- COMMENT: 60eea6892c654dff 27 K5VaIcg7u0r4ld0aJzpfv7aP8D8 Figure 4A–4C and Figure S3A–S3B ------- COMMENT: 60eea6892c654dff 28 s7cvhfiYiveaAA2KzWLwMqq+fAs fig 4c ------- COMMENT: 60eea6892c654dff 29 s7cvhfiYiveaAA2KzWLwMqq+fAs fig 4c ------- COMMENT: 60eea6892c654dff 31 s7cvhfiYiveaAA2KzWLwMqq+fAs fig 4c ------- COMMENT: 60eea6892c654dff 32 Oqlo3QSDMMKz9kpIJi8tYe081g0 fig S3F ------- COMMENT: 60eea6892c654dff 34 oTj0fwCjRF74pr37QtPclhtyves Cyk3-GFP localized to the CR and division site during cytokinesis, and it was retained at new ends immediately following cell division (Figure 4F). ------- COMMENT: 60eea6892c654dff 35 oTj0fwCjRF74pr37QtPclhtyves Cyk3-GFP localized to the CR and division site during cytokinesis, and it was retained at new ends immediately following cell division (Figure 4F). ------- COMMENT: 60eea6892c654dff 36 iFICvZwdug6VOnnB3nTRaE8NLQw Fic1 most likely functions during late stages of cytokinesis. In line with this idea, the percentage of fic1D cells that had undergone ingression but were still joined at their division sites was more than four times that of wild-type cells (Figure 5A–5B). ------- COMMENT: 60eea6892c654dff 37 AdDhS2N9tB8afnJ17p4++LGbme4 Consistent with early cytokinesis events proceeding appropri- ately without Fic1, time-lapse imaging of myosin regulatory light chain Rlc1-GFP [47,48] along with spindle pole body marker Sid4-GFP revealed that the CR formed and constricted normally in fic1D cells (Figure 5C–5D). However, at the termination of CR constriction, parts of the CR persisted at the division plane (Figure 5E–5G and Figure S4D). ------- COMMENT: 60eea6892c654dff 38 M3D2AOvNnJfUwSqbT/vX4I/4FKg fig 4c/ figure 6a ------- COMMENT: 60eea6892c654dff 39 sBQbtCxRAo7/lssqY9vfSiREPbA Loss of Eng1 or its cooperating glucanase, Agn1 [34], resulted in high percentages of monopolar growth (Figure 6C–6D and Figure S5A) ------- COMMENT: 60eea6892c654dff 40 sBQbtCxRAo7/lssqY9vfSiREPbA Loss of Eng1 or its cooperating glucanase, Agn1 [34], resulted in high percentages of monopolar growth (Figure 6C–6D and Figure S5A) ------- COMMENT: 60eea6892c654dff 45 7EDphuEZjmjbCYjkWmHN9uys0E8 Figure S5B ------- COMMENT: 60eea6892c654dff 46 7EDphuEZjmjbCYjkWmHN9uys0E8 Figure S5B ------- COMMENT: 60eea6892c654dff 47 f12W8a/gwOgs6V1XLesxx5zxekg Cells lacking Fic1 or its interacting partners Cyk3 or Imp2 were significantly more invasive than wild-type cells (Figure 9A–9B). ------- COMMENT: 60eea6892c654dff 48 f12W8a/gwOgs6V1XLesxx5zxekg Cells lacking Fic1 or its interacting partners Cyk3 or Imp2 were significantly more invasive than wild-type cells (Figure 9A–9B). ------- COMMENT: 60eea6892c654dff 49 f12W8a/gwOgs6V1XLesxx5zxekg Cells lacking Fic1 or its interacting partners Cyk3 or Imp2 were significantly more invasive than wild-type cells (Figure 9A–9B). ------- COMMENT: 60eea6892c654dff 50 FzkHUWB6Ua4w5czoLSGaWxT1Ymk In addition to these strains, we found other cytokinesis mutants exhibiting high degrees of monopolar growth (spn1D, cdc7-24, and vps24D) to also be highly invasive and to form pseudohyphal projections into 2% agar (Figure 9A–9B and Figure S7A) ------- COMMENT: 6101d4a4d449a1cc 11 CEYEHF75n9wdEGdDukoGlXEbFJg (comment: CHECK FYPO:0007229) ------- COMMENT: 61148469370725b8 11 +NdYzbpE/iHAapozd10siWs0H38 (comment: nrd1delta and cdc4-8) ------- COMMENT: 61148469370725b8 20 xfC0MNZ4PejfpbsBIS9jEWWoRDs "Thus, Nrd1 di- rectly binds with Cdc4 mRNA in vivo and in vitro" ------- COMMENT: 61148469370725b8 25 Hs6/5ONBSV4p6V72kaqANUGhVX4 fig 4F ------- COMMENT: 61148469370725b8 26 Hs6/5ONBSV4p6V72kaqANUGhVX4 fig 4F ------- COMMENT: 61148469370725b8 45 azZ9d+Qj9DjEc7pbCblLANZLvAE Notably, Pmk1, the mitogen-activated protein kinase (MAPK), which regulates cell integrity (Toda et al., 1996; Sugiura et al., 1999; Sugiura et al., 2003), directly phosphorylates Nrd1, thereby negatively regulating the ac- tivity of Nrd1 to bind to and stabilize Cdc4 mRNA. We propose that the MAPK-dependent phosphorylation of the RNA-binding protein Nrd1 may serve as a novel mechanism for the regulation of myosin mRNA and cytokinesis in fission yeast. ------- COMMENT: 6115bf31740b991f 5 dJnEbe0FVIO0QxtT2YTUUpCl+bA (comment: CHECK does not grow at high temperature, defective pre-mRNA splicing, assayed_using SPBC1778.02 | assayed_using SPAC227.16C | assayed_using SPBP16F5.02 ------- COMMENT: 6115bf31740b991f 9 x47QLN0vDUeOLmxPABLlCG44BCs (comment: CHECK column_17 Sde2UBL) ------- COMMENT: 6115bf31740b991f 10 9dVSigR72XCDTzcRkty8o+a5Q30 (comment: CHECK Intron-Specific pre-mRNA Splicing) ------- COMMENT: 6115bf31740b991f 11 DCLCa3b+c0ffbErB92NNyjqOwII (comment: CHECK decreased cell population growth at high temperature) ------- COMMENT: 6115bf31740b991f 20 tMyaZ+lX+KC67HxTD2PBR9rrPMM does not complement sde2Δ, defective in telomeric silencing and genome stability ------- COMMENT: 6115bf31740b991f 21 R5+OgfAGUfgE3XXcBFRmBDNBfNQ normal processing, protein very stable, complements partially sde2Δ ------- COMMENT: 6115bf31740b991f 22 LbXzGm25n4BV8qsKgP0uB0oVBWY normal processing, complements partially sde2Δ ------- COMMENT: 6115bf31740b991f 23 dAmPAhZUha1yWzG3cL3jqZD+sVU processing defective, does not complement sde2Δ ------- COMMENT: 6115bf31740b991f 24 xYYCtqEWxONTBtOjxQ0lsdeZOM4 fig 1D does not complement sde2Δ ------- COMMENT: 6115bf31740b991f 25 E0V4RSJlpnoqyfAsj9GqzjmNg+8 fig 1D complements partially sde2Δ ------- COMMENT: 6115bf31740b991f 26 dNCaJK6/qfKx17YizL5CiSwZpzk normal processing, protein very stable, does not complement sde2Δ ------- COMMENT: 6115bf31740b991f 27 0Ce5iJlZQpcP6wgOpoLekuoTzQw normal processing, protein unstable, complements partially sde2Δ ------- COMMENT: 6115bf31740b991f 28 0Ce5iJlZQpcP6wgOpoLekuoTzQw normal processing, protein unstable, complements partially sde2Δ ------- COMMENT: 6115bf31740b991f 29 v6S8D+t0UXQ8HSv4JpKzEyZzp+E normal processing, protein stable, complements partially sde2Δ ------- COMMENT: 6115bf31740b991f 30 R5+OgfAGUfgE3XXcBFRmBDNBfNQ normal processing, protein very stable, complements partially sde2Δ ------- COMMENT: 6115bf31740b991f 31 LbXzGm25n4BV8qsKgP0uB0oVBWY normal processing, complements partially sde2Δ ------- COMMENT: 6115bf31740b991f 32 v6S8D+t0UXQ8HSv4JpKzEyZzp+E normal processing, protein stable, complements partially sde2Δ ------- COMMENT: 6115bf31740b991f 33 R5+OgfAGUfgE3XXcBFRmBDNBfNQ normal processing, protein very stable, complements partially sde2Δ ------- COMMENT: 6115bf31740b991f 34 ep3+6plo5mpQ0M8Ilo/6AdsH2g0 Fig 3C decreased cell growth, normal processing, protein very stable, complements partially sde2Δ; over expression causes growth defect in hub1-1 strain, defective in telomeric silencing and genome stability ------- COMMENT: 6115bf31740b991f 35 LbXzGm25n4BV8qsKgP0uB0oVBWY normal processing, complements partially sde2Δ ------- COMMENT: 6115bf31740b991f 36 dAmPAhZUha1yWzG3cL3jqZD+sVU processing defective, does not complement sde2Δ ------- COMMENT: 6115bf31740b991f 37 R5+OgfAGUfgE3XXcBFRmBDNBfNQ normal processing, protein very stable, complements partially sde2Δ ------- COMMENT: 6115bf31740b991f 38 LPu6UVqTmSvOjESSBvCl21SjGSg normal processing, protein unstable,complements partially sde2Δ ------- COMMENT: 6115bf31740b991f 39 v6S8D+t0UXQ8HSv4JpKzEyZzp+E normal processing, protein stable, complements partially sde2Δ ------- COMMENT: 6115bf31740b991f 40 R5+OgfAGUfgE3XXcBFRmBDNBfNQ normal processing, protein very stable, complements partially sde2Δ ------- COMMENT: 6115bf31740b991f 41 LbXzGm25n4BV8qsKgP0uB0oVBWY normal processing, complements partially sde2Δ ------- COMMENT: 6115bf31740b991f 42 LbXzGm25n4BV8qsKgP0uB0oVBWY normal processing, complements partially sde2Δ ------- COMMENT: 6115bf31740b991f 43 LbXzGm25n4BV8qsKgP0uB0oVBWY normal processing, complements partially sde2Δ ------- COMMENT: 6115bf31740b991f 44 84MVhG08thf1SIMwjVyNVxy8MCU reduced processing, complements partially sde2Δ ------- COMMENT: 6115bf31740b991f 45 dAmPAhZUha1yWzG3cL3jqZD+sVU processing defective, does not complement sde2Δ ------- COMMENT: 6115bf31740b991f 46 ELXhPRiDgZ4XsRJz2txr99dbFlk Fig EV2B normal processing, complements sde2Δ ------- COMMENT: 6115bf31740b991f 47 PoBFjnvxqLIy3x6E9HLIb/eRvNU Fig EV2B normal processing, complements sde2Δ ------- COMMENT: 6115bf31740b991f 48 Ne57WIRdC+/LguBx1qzRGD/K2EY Fig EV2B processing defective, does not complement sde2Δ ------- COMMENT: 6115bf31740b991f 49 Ne57WIRdC+/LguBx1qzRGD/K2EY Fig EV2B processing defective, does not complement sde2Δ ------- COMMENT: 6115bf31740b991f 50 Ne57WIRdC+/LguBx1qzRGD/K2EY Fig EV2B processing defective, does not complement sde2Δ ------- COMMENT: 6115bf31740b991f 51 Ne57WIRdC+/LguBx1qzRGD/K2EY Fig EV2B processing defective, does not complement sde2Δ ------- COMMENT: 6115bf31740b991f 124 Awr43TvOx7VAKrlcdARjl0rI3is Fig 5B; Appendix Fig S5A and B ------- COMMENT: 6115bf31740b991f 126 PrFoE0ejyMFOQbituT4ftGEOtxI (comment: CHECK endo mutant does not cleave Sde2 precursor) ------- COMMENT: 6115bf31740b991f 127 PrFoE0ejyMFOQbituT4ftGEOtxI (comment: CHECK endo mutant does not cleave Sde2 precursor) ------- COMMENT: 6115bf31740b991f 128 Y7eS8uZ3Wbede8RocLgmfyPua08 (comment: CHECK assayed_using SPBC1778.02 | assayed_using SPAC227.16C | assayed_using SPBP16F5.02) ------- COMMENT: 6115bf31740b991f 130 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 6115bf31740b991f 132 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 6115bf31740b991f 135 MNjN+o2AJtbe61l/L7T/TqpiTdo Appendix Fig S2A ------- COMMENT: 6115bf31740b991f 136 Edc6amlu5ePaAOzpaakQ7CtK8co Fig 2A &B ------- COMMENT: 6115bf31740b991f 137 6pSAHAPl4EEMTDqkfHAb9E42zmg Fig 2D ------- COMMENT: 6115bf31740b991f 138 6pSAHAPl4EEMTDqkfHAb9E42zmg Fig 2D ------- COMMENT: 6115bf31740b991f 139 jQgjUTZlbUvSBMe09qTM+gRgdzI Fig 3E (comment: CHECK N-end rule pathway substrate assayed using LysSde2-C N-end rule substrate pro-obo/term-requests/119/ ) ------- COMMENT: 6115bf31740b991f 140 Capqh8i4xCwTDBqW71dyQVqxY5o Fig 3E (comment: CHECK N-end rule pathway substrate assayed using LysSde2-C N-end rule substrate pro-obo/term-requests/119/ ) ------- COMMENT: 6115bf31740b991f 141 RixlvlgTmMBOZ93/xdU305iyOZI Fig 4A ------- COMMENT: 6115bf31740b991f 143 RKZSGuEugZBmCUmnb2MafPv15M8 (Fig 7A) ------- COMMENT: 6115bf31740b991f 144 Gu5JDDG4c2bjWl8ejggcAGhe6XA Fig 8B (comment: CHECK in spliceosome) ------- COMMENT: 6115bf31740b991f 145 9dVSigR72XCDTzcRkty8o+a5Q30 (comment: CHECK Intron-Specific pre-mRNA Splicing) ------- COMMENT: 611cd009f8477dea 1 W7T+x+zh3RDJTwytn9g6EZsqskA (comment: CHECK plasma membrane fusion during conjugation) ------- COMMENT: 611cd009f8477dea 12 iw2gjyoS89mRII4Ivf7aNgmH+uQ (comment: CHECK Fusion domain) ------- COMMENT: 611cd009f8477dea 53 W7T+x+zh3RDJTwytn9g6EZsqskA (comment: CHECK plasma membrane fusion during conjugation) ------- COMMENT: 612939864abd598a 7 0H9lYxlMPZIXChXEfjihRJKETxU (comment: CHECK exists during anaphase A and anaphase B) ------- COMMENT: 612939864abd598a 9 70eRcbGNpIZCwcXI2ZdGSDiab00 ------- COMMENT: 612939864abd598a 10 PhvSciomwOd1i/C5DFUPLMHkI1E ------- COMMENT: 612939864abd598a 15 WIQobKVmOSwF4pnw3zyHiiYk2Lk fig 1A ------- COMMENT: 612939864abd598a 17 U/PvCZ3/sYCTc+VkXd+IsT2uQcI fig 1B ------- COMMENT: 612939864abd598a 18 U/PvCZ3/sYCTc+VkXd+IsT2uQcI fig 1B ------- COMMENT: 612939864abd598a 20 iO1gCduMaqiACHUtMrpoyFz8iiQ fig 1D-G ------- COMMENT: 612939864abd598a 22 n4BRuKw5fXg/rThpIqLYdJH6qgM fig 1d-g ------- COMMENT: 612939864abd598a 23 n4BRuKw5fXg/rThpIqLYdJH6qgM fig 1G-G ------- COMMENT: 612939864abd598a 25 px7NMsyIb/ZjsX/gQXWqIuMmhkA fig 1H ------- COMMENT: 612939864abd598a 29 ZLH7eviy+wV9DDCPfzTodgZqesw fig S1a ------- COMMENT: 612939864abd598a 32 j8lx0cjXOogtvtAy6trm2XWxJ9Q fig 2A ------- COMMENT: 612939864abd598a 34 j8lx0cjXOogtvtAy6trm2XWxJ9Q fig 2A ------- COMMENT: 612939864abd598a 36 0/WZp/jT6BxuuZIo+lSkpZ/seuc fig 2A ------- COMMENT: 612939864abd598a 48 p8SgknyuBDrKpf5LkPKSpMYQ37k fig 3E ------- COMMENT: 612939864abd598a 49 p8SgknyuBDrKpf5LkPKSpMYQ37k fig 3E ------- COMMENT: 613296d2e91f5cb0 19 IPSVzZxrG+3Zb4mIAI3MjFbkDAw figure 5D, figure 6 ------- COMMENT: 613296d2e91f5cb0 25 gn6F4FHtkCDJbdvmjjjrcc3kntU fig 3C ------- COMMENT: 613296d2e91f5cb0 26 Pzw2cRXrCOZm9kxcEyVo//AMWwk Figure 3F ------- COMMENT: 613296d2e91f5cb0 27 I5QTtJH2zeMqyUAmANukuS5L1ec figure 3F ------- COMMENT: 613296d2e91f5cb0 28 I5QTtJH2zeMqyUAmANukuS5L1ec figure 3F ------- COMMENT: 613296d2e91f5cb0 29 I5QTtJH2zeMqyUAmANukuS5L1ec figure 3F ------- COMMENT: 613296d2e91f5cb0 30 I5QTtJH2zeMqyUAmANukuS5L1ec figure 3F ------- COMMENT: 613296d2e91f5cb0 31 I5QTtJH2zeMqyUAmANukuS5L1ec figure 3F ------- COMMENT: 613296d2e91f5cb0 32 I5QTtJH2zeMqyUAmANukuS5L1ec figure 3F ------- COMMENT: 613296d2e91f5cb0 33 l165mgamoT9Y90wthcjZQM/bn5E table 1 ------- COMMENT: 613296d2e91f5cb0 34 l165mgamoT9Y90wthcjZQM/bn5E table 1 ------- COMMENT: 613296d2e91f5cb0 35 l165mgamoT9Y90wthcjZQM/bn5E table 1 ------- COMMENT: 613296d2e91f5cb0 36 l165mgamoT9Y90wthcjZQM/bn5E table 1 ------- COMMENT: 613296d2e91f5cb0 37 l165mgamoT9Y90wthcjZQM/bn5E table 1 ------- COMMENT: 613296d2e91f5cb0 38 l165mgamoT9Y90wthcjZQM/bn5E table 1 ------- COMMENT: 613296d2e91f5cb0 41 l165mgamoT9Y90wthcjZQM/bn5E table 1 ------- COMMENT: 613296d2e91f5cb0 42 l165mgamoT9Y90wthcjZQM/bn5E table 1 ------- COMMENT: 613296d2e91f5cb0 43 l165mgamoT9Y90wthcjZQM/bn5E table 1 ------- COMMENT: 613296d2e91f5cb0 44 l165mgamoT9Y90wthcjZQM/bn5E table 1 ------- COMMENT: 613296d2e91f5cb0 47 Jlk1V51+Ro3/iw8g0EtgXjlKa3U fig 5 a ------- COMMENT: 613296d2e91f5cb0 53 4nRRb33mfWtp1BNrtVn8iXajKx0 Figure 4 F ------- COMMENT: 613296d2e91f5cb0 54 CtFq8HIGkVRobj0eGtdCa+CQdGc fig5a ------- COMMENT: 613296d2e91f5cb0 58 /OPf8xxNh4UviwnKWubBlSq80Tc figure 4 G ------- COMMENT: 613296d2e91f5cb0 70 nUCcEDoowWdBc8w5z3A4wbzAAHc Figure 1F ------- COMMENT: 613296d2e91f5cb0 71 gn6F4FHtkCDJbdvmjjjrcc3kntU fig 3 C ------- COMMENT: 613296d2e91f5cb0 72 gn6F4FHtkCDJbdvmjjjrcc3kntU fig 3 C ------- COMMENT: 613296d2e91f5cb0 73 wyxuKnsj8sK2NMI1h45DWqqFjmo figure 4 G (comment: localizes as a dot rather than a disk) ------- COMMENT: 613296d2e91f5cb0 74 ta7Nx6I9bRHFYJqtbPHryfURqug Supplemental Figure S4B ------- COMMENT: 613296d2e91f5cb0 76 MgwR+yugGADz9INO65bJ3JOgg3E Supplemental Figure S4F and Table 2 ------- COMMENT: 613296d2e91f5cb0 122 Zw8p4mHE3ohIpi3n5NIRKoDWSE8 table 1, fig 3 C ------- COMMENT: 613296d2e91f5cb0 123 IPSVzZxrG+3Zb4mIAI3MjFbkDAw figure 5 d/ figure 6 ------- COMMENT: 614edec10b9e3e6a 7 Q1uJnSorM6NB2KPbDPnIJ2lmevc (Fig. 6) (comment: CHECK increased or premature) ------- COMMENT: 614edec10b9e3e6a 8 +xHRy6xd8RJiHo7OUEpfD/sn8ag fig1b ------- COMMENT: 614edec10b9e3e6a 9 Q1uJnSorM6NB2KPbDPnIJ2lmevc (Fig. 6) (comment: CHECK increased or premature) ------- COMMENT: 614edec10b9e3e6a 10 pXbYGKf3EtJB2OMdyRS2AlqAdvY (Fig. 8, B and C) (comment: CHECK increased or premature) ------- COMMENT: 614edec10b9e3e6a 11 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 614edec10b9e3e6a 12 25xeFMyY5v6U2lyk9+h2xOM+NIQ Fig. 3, A and B; Fig. S5 ------- COMMENT: 614edec10b9e3e6a 13 A2fKWvr4XCq3F8cx8m9WBs5469s (Fig. 4, C and D) ------- COMMENT: 614edec10b9e3e6a 15 pu8G6UmP4pIIrUw8TvJdzyIRy6M (Fig. 1B) ------- COMMENT: 614edec10b9e3e6a 16 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 614edec10b9e3e6a 17 llms4H6U4rwVKJUTwUJtwZQIs4M (Fig. 1D) ------- COMMENT: 614edec10b9e3e6a 18 llms4H6U4rwVKJUTwUJtwZQIs4M (Fig. 1D) ------- COMMENT: 614edec10b9e3e6a 19 FmkOCgcuOlbLDVk2YrKUCxJRKaQ Fig S1. (comment: Assayed by assaying depletion of securin from spindle) ------- COMMENT: 614edec10b9e3e6a 20 hFMJX62U1HlyiAD5BO9LBdDbLlI (Fig. 7, B and C) ------- COMMENT: 614edec10b9e3e6a 21 IIFYeJtgDxRrIRGW6ssii6mP2Uw (Fig. 7C) ------- COMMENT: 614edec10b9e3e6a 22 lC9MJNTC0RK0K7e90ci4GkCQiNw fig 9A ------- COMMENT: 614edec10b9e3e6a 23 lC9MJNTC0RK0K7e90ci4GkCQiNw fig 9A ------- COMMENT: 614edec10b9e3e6a 24 lC9MJNTC0RK0K7e90ci4GkCQiNw fig 9A ------- COMMENT: 614edec10b9e3e6a 25 lC9MJNTC0RK0K7e90ci4GkCQiNw fig 9A ------- COMMENT: 614edec10b9e3e6a 26 lC9MJNTC0RK0K7e90ci4GkCQiNw fig 9A ------- COMMENT: 614edec10b9e3e6a 27 lC9MJNTC0RK0K7e90ci4GkCQiNw fig 9A ------- COMMENT: 614edec10b9e3e6a 28 lC9MJNTC0RK0K7e90ci4GkCQiNw fig 9A ------- COMMENT: 614edec10b9e3e6a 29 lC9MJNTC0RK0K7e90ci4GkCQiNw fig 9A ------- COMMENT: 614edec10b9e3e6a 30 lC9MJNTC0RK0K7e90ci4GkCQiNw fig 9A ------- COMMENT: 614edec10b9e3e6a 31 lC9MJNTC0RK0K7e90ci4GkCQiNw fig 9A ------- COMMENT: 614edec10b9e3e6a 32 lC9MJNTC0RK0K7e90ci4GkCQiNw fig 9A ------- COMMENT: 614edec10b9e3e6a 33 lC9MJNTC0RK0K7e90ci4GkCQiNw fig 9A ------- COMMENT: 614edec10b9e3e6a 34 lC9MJNTC0RK0K7e90ci4GkCQiNw fig 9A ------- COMMENT: 614edec10b9e3e6a 35 lC9MJNTC0RK0K7e90ci4GkCQiNw fig 9A ------- COMMENT: 614edec10b9e3e6a 36 QejlCf90C5/3wQnCwYPm0Hu7jPw fig 9a, there other evidence elsewhere but we don't have this annotation on mad2 at present... ------- COMMENT: 614edec10b9e3e6a 37 pu8G6UmP4pIIrUw8TvJdzyIRy6M (Fig. 1B) ------- COMMENT: 615084ae39833661 2 Mk0Iln8V7ZQg3sL3I7PiYU0OD+Q (comment: mcs6 requires mcs2 for CTD kinase activity but not cyclin-dependent kinase activating kinase activity. mcs2 does not cycle throughout the cell cycle.) ------- COMMENT: 615084ae39833661 16 yVlFzPSGSZVPnlhuT/AuBgkBmxU (comment: Csk1 activated both the monomeric and the Mcs2-bound forms of Mcs6) ------- COMMENT: 615084ae39833661 17 XVELwiT43DQCaV3DbCXxqmw6svA Surprisingly, Csk1 also activated Cdc2 in complexes with either Cdc13 or Cig2 cyclins. ------- COMMENT: 615730f8699178ab 5 eCMi+2+M79gobfQ4X9J4EIrKsGA Fig 1 a ------- COMMENT: 615730f8699178ab 6 4TiyK0t5N/MB/LpQQ3rFGLFQMIY Fig 1a ------- COMMENT: 615730f8699178ab 9 07BIqIZk7Gu97uo3WamPvVnzg5M Figure 1C. Such a band was not observed for D193A and T208A mutants, confirming that they are indeed kinase dead ------- COMMENT: 615730f8699178ab 11 Mlkx4WvvwXtl6qKvAfBb9ghebJY in vitro autophosphorylation activity of Atg1 from atg11D mutant was almost undetectable (Figure 1D ------- COMMENT: 615730f8699178ab 24 S4njJCU/lJ5aE03hYz2msWvEhBA we found that in S. pombe, Atg1 from atg13D, atg17D, or atg101D mutant exhibited autophosphorylation activities simi- lar to that of Atg1 from wild type ------- COMMENT: 616a9b2185a857ce 1 JyWba+xIS9l1zUM/LlYLytC77xc Figure 5B, Figure 5—figure supplement 1C ------- COMMENT: 616a9b2185a857ce 2 24vLI/pGJfLn0pmTMN/bBFrc8rM Figure 6—figure supplement 1 ------- COMMENT: 616a9b2185a857ce 3 JyWba+xIS9l1zUM/LlYLytC77xc Figure 5B, Figure 5—figure supplement 1C ------- COMMENT: 616a9b2185a857ce 4 JyWba+xIS9l1zUM/LlYLytC77xc Figure 5B, Figure 5—figure supplement 1C ------- COMMENT: 616a9b2185a857ce 5 JyWba+xIS9l1zUM/LlYLytC77xc Figure 5B, Figure 5—figure supplement 1C ------- COMMENT: 616a9b2185a857ce 6 24vLI/pGJfLn0pmTMN/bBFrc8rM Figure 6—figure supplement 1 ------- COMMENT: 616a9b2185a857ce 7 E03F+HjAVcjD6gPCz6HJ6sFCR3k Figure 1D, Figure 1—figure supplement 1; spot test and survival assay ------- COMMENT: 616a9b2185a857ce 8 E03F+HjAVcjD6gPCz6HJ6sFCR3k Figure 1D, Figure 1—figure supplement 1; spot test and survival assay ------- COMMENT: 616a9b2185a857ce 9 5TKVTLmYe+TetzyA8aaFbGPOQB8 Figure 7C,D; spot test and survival assay ------- COMMENT: 616a9b2185a857ce 10 5TKVTLmYe+TetzyA8aaFbGPOQB8 Figure 7C,D; spot test and survival assay ------- COMMENT: 616a9b2185a857ce 11 5TKVTLmYe+TetzyA8aaFbGPOQB8 Figure 7C,D; spot test and survival assay ------- COMMENT: 616a9b2185a857ce 12 nXY0al/Vw6Ts1kl1IrzX+c9h1z4 Figure 7—figure supplement 1B; spot test ------- COMMENT: 616a9b2185a857ce 13 nXY0al/Vw6Ts1kl1IrzX+c9h1z4 Figure 7—figure supplement 1B; spot test ------- COMMENT: 616a9b2185a857ce 14 nXY0al/Vw6Ts1kl1IrzX+c9h1z4 Figure 7—figure supplement 1B; spot test ------- COMMENT: 616a9b2185a857ce 15 K/tCIoXgrn3rIMFiSSgr7L1zn0Q Figure 1—figure supplement 1A, Figure 7C,D, Figure 7—figure supplement 1A; spot test and survival assay ------- COMMENT: 616a9b2185a857ce 16 qufjII6Low4XnThXvdOaW2HN3d4 Figure 1—figure supplement 1A; spot test ------- COMMENT: 616a9b2185a857ce 17 qufjII6Low4XnThXvdOaW2HN3d4 Figure 1—figure supplement 1A; spot test ------- COMMENT: 616a9b2185a857ce 24 v4E+BKkG97igckK9RzC63j3M7c8 (comment: in complex with Swi5) Figure 6 ------- COMMENT: 616a9b2185a857ce 25 wXnnCweZhOQMUHNaZYWjbmtcDBA (comment: in complex with Sfr1) Figure 6 ------- COMMENT: 616a9b2185a857ce 26 jbm58rFSz1Gxdgrs7oxckM7gK8M inferred from combination of in vitro assay and phenotypes; Figures 1 & 5, including supplements ------- COMMENT: 616a9b2185a857ce 27 jbm58rFSz1Gxdgrs7oxckM7gK8M inferred from combination of in vitro assay and phenotypes; Figures 1 & 5, including supplements ------- COMMENT: 616a9b2185a857ce 28 tYIr1lFwJ9OLW+jMk8No3+s7nzg Figure 7A,B ------- COMMENT: 616a9b2185a857ce 29 tYIr1lFwJ9OLW+jMk8No3+s7nzg Figure 7A,B ------- COMMENT: 616a9b2185a857ce 30 tYIr1lFwJ9OLW+jMk8No3+s7nzg Figure 7A,B ------- COMMENT: 6174b6ceda38fefa 4 PwScBBgg2k2hc3uFUfvaMNLs91k (figure 7b) the absence of Mas5 also blocked the accumulation of Hsp104-GFP into NuRs (Figure 7b), ------- COMMENT: 6186ba197a53e798 1 t/wACwcbTUYXgkG85OvIjzdnVJo Figure 1, 2 and 4; Supplementary Fig S1, S3 and S5 ------- COMMENT: 6186ba197a53e798 2 t/wACwcbTUYXgkG85OvIjzdnVJo Figure 1, 2 and 4; Supplementary Fig S1, S3 and S5 ------- COMMENT: 6186ba197a53e798 3 t/wACwcbTUYXgkG85OvIjzdnVJo Figure 1, 2 and 4; Supplementary Fig S1, S3 and S5 ------- COMMENT: 6186ba197a53e798 5 wKNXoAfsDTzbeOcNRTT63Cik10o Figure 2 ------- COMMENT: 6186ba197a53e798 6 wKNXoAfsDTzbeOcNRTT63Cik10o Figure 2 ------- COMMENT: 6186ba197a53e798 7 wKNXoAfsDTzbeOcNRTT63Cik10o Figure 2 ------- COMMENT: 6186ba197a53e798 8 wKNXoAfsDTzbeOcNRTT63Cik10o Figure 2 ------- COMMENT: 6186ba197a53e798 9 wKNXoAfsDTzbeOcNRTT63Cik10o Figure 2 ------- COMMENT: 6186ba197a53e798 10 wKNXoAfsDTzbeOcNRTT63Cik10o Figure 2 ------- COMMENT: 6186ba197a53e798 11 kcTCzwG+ta365TScD94fn3bM5Gg Figure 4 and Supplementary Fig S5 ------- COMMENT: 6186ba197a53e798 12 kcTCzwG+ta365TScD94fn3bM5Gg Figure 4 and Supplementary Fig S5 ------- COMMENT: 6186ba197a53e798 13 kcTCzwG+ta365TScD94fn3bM5Gg Figure 4 and Supplementary Fig S5 ------- COMMENT: 6186ba197a53e798 14 kcTCzwG+ta365TScD94fn3bM5Gg Figure 4 and Supplementary Fig S5 ------- COMMENT: 6186ba197a53e798 15 kcTCzwG+ta365TScD94fn3bM5Gg Figure 4 and Supplementary Fig S5 ------- COMMENT: 6186ba197a53e798 16 kcTCzwG+ta365TScD94fn3bM5Gg Figure 4 and Supplementary Fig S5 ------- COMMENT: 6186ba197a53e798 17 kcTCzwG+ta365TScD94fn3bM5Gg Figure 4 and Supplementary Fig S5 ------- COMMENT: 6186ba197a53e798 18 kcTCzwG+ta365TScD94fn3bM5Gg Figure 4 and Supplementary Fig S5 ------- COMMENT: 6186ba197a53e798 19 kcTCzwG+ta365TScD94fn3bM5Gg Figure 4 and Supplementary Fig S5 ------- COMMENT: 6186ba197a53e798 28 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 6186ba197a53e798 29 1xq33/x626SbYZ9uLRqQx2xp1iM Figure 5; rbp1 also Figure 7 ------- COMMENT: 6186ba197a53e798 30 1xq33/x626SbYZ9uLRqQx2xp1iM Figure 5; rbp1 also Figure 7 ------- COMMENT: 6186ba197a53e798 31 Wbgv3NeUdCVpvVbBvg67PbqTwOw Supplementary Fig S7 ------- COMMENT: 6186ba197a53e798 32 Wbgv3NeUdCVpvVbBvg67PbqTwOw Supplementary Fig S7 ------- COMMENT: 6186ba197a53e798 33 Wbgv3NeUdCVpvVbBvg67PbqTwOw Supplementary Fig S7 ------- COMMENT: 6186ba197a53e798 34 Wbgv3NeUdCVpvVbBvg67PbqTwOw Supplementary Fig S7 ------- COMMENT: 6186ba197a53e798 35 Wbgv3NeUdCVpvVbBvg67PbqTwOw Supplementary Fig S7 ------- COMMENT: 6186ba197a53e798 36 Wbgv3NeUdCVpvVbBvg67PbqTwOw Supplementary Fig S7 ------- COMMENT: 6186ba197a53e798 37 75C2HDt3gruEDA6xicDJYuI1+1A Figure 6 and Supplementary Fig S8 ------- COMMENT: 6186ba197a53e798 38 75C2HDt3gruEDA6xicDJYuI1+1A Figure 6 and Supplementary Fig S8 ------- COMMENT: 6186ba197a53e798 39 75C2HDt3gruEDA6xicDJYuI1+1A Figure 6 and Supplementary Fig S8 ------- COMMENT: 6186ba197a53e798 40 SS5A/TOjGoqO5dbWJ6Ds6VA9ZjU Supplementary Fig S8 ------- COMMENT: 6186ba197a53e798 41 SS5A/TOjGoqO5dbWJ6Ds6VA9ZjU Supplementary Fig S8 ------- COMMENT: 6186ba197a53e798 42 SS5A/TOjGoqO5dbWJ6Ds6VA9ZjU Supplementary Fig S8 ------- COMMENT: 619cffb818489e7f 2 QlYiilziIYDxaYVlAxT5lPmTmys ------- COMMENT: 619cffb818489e7f 16 LVBNUYDl1ngpSSmjL4Si9f/DFrM ------- COMMENT: 619cffb818489e7f 30 LVBNUYDl1ngpSSmjL4Si9f/DFrM ------- COMMENT: 61b5b08d53bec31b 1 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 61b5b08d53bec31b 2 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 61b5b08d53bec31b 3 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 61b5b08d53bec31b 4 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 61b5b08d53bec31b 5 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 61b5b08d53bec31b 6 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 61b5b08d53bec31b 7 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 61b5b08d53bec31b 8 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 61b5b08d53bec31b 9 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 61b5b08d53bec31b 10 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ----- COMMENT: 61b5b08d53bec31b 11 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 12 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 13 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 14 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 15 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 16 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 17 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 18 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 19 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 20 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 21 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 22 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 23 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 24 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 25 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 26 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 27 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 28 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 29 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 30 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 31 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 32 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 33 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 34 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 35 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 36 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 37 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 38 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 39 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 40 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 41 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 42 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 43 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 44 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 45 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 46 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 47 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 48 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 49 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 50 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 51 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 52 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 53 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 54 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 55 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 56 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 57 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 58 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 59 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 60 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61b5b08d53bec31b 61 BCT25wJvpLwsSnBDCDQXMVETx5s (comment: CHECK Table ?) ------- COMMENT: 61c5a0ac6ec56979 15 j9EpwlF+cOBcOFkpLAsx6/Icu7M (comment: temperature permissive for ts cdc17-K42) ------- COMMENT: 61c5a0ac6ec56979 16 j9EpwlF+cOBcOFkpLAsx6/Icu7M (comment: temperature permissive for ts cdc17-K42) ------- COMMENT: 61c5a0ac6ec56979 19 j9EpwlF+cOBcOFkpLAsx6/Icu7M (comment: temperature permissive for ts cdc17-K42) ------- COMMENT: 61e3a10a189ba939 31 2PgFnL2DO9EPyL5UIxRKNJHmtNM (comment: maybe not shown strongly in this paper but I'm trying to get the git genes annotated to this term because pka1 phosphorylates rst2 which excludes rst2 from the nucleus. rst2 when in the nucleus activates ste11 transcription.) ------- COMMENT: 61e3a10a189ba939 32 2PgFnL2DO9EPyL5UIxRKNJHmtNM comment: maybe not shown strongly in this paper but I'm trying to get the git genes annotated to this term because pka1 phosphorylates rst2 which excludes rst2 from the nucleus. rst2 when in the nucleus activates ste11 transcription.) ------- COMMENT: 62345443623a0faf 24 fnQ4Acq2nPZO6IZNEbV5I8w+pXs supp fig? ------- COMMENT: 624d205232adf87e 1 cO/d/vOh3z9Bw3qq7tlKAyOnT9E Additional file 1: Fig. S1b, c ------- COMMENT: 624d205232adf87e 2 Ioj3QUwIQAe2ajGHOG0fd4T7540 (Fig. 1c) ------- COMMENT: 624d205232adf87e 3 cO/d/vOh3z9Bw3qq7tlKAyOnT9E Additional file 1: Fig. S1b, c ------- COMMENT: 624d205232adf87e 4 cO/d/vOh3z9Bw3qq7tlKAyOnT9E Additional file 1: Fig. S1b, c ------- COMMENT: 624d205232adf87e 6 +6Lcr2d1v0ewZ3DLwwRAwXJ8/DY In sharp contrast, the H3K9me2 levels remained constant in leo1∆ cells throughout G0 phase (Fig. 2; Additional file 2: Fig. S2 ------- COMMENT: 627a2efc48957a76 1 inH3LPlpLY7xPLaUnnHc45HTmZA Supplementary Figure S3 ------- COMMENT: 627a2efc48957a76 2 ZGMZvcYvzp8RGnnUgdqMjmArVL0 Supplementary Fig. S3 ------- COMMENT: 627a2efc48957a76 3 ZGMZvcYvzp8RGnnUgdqMjmArVL0 Supplementary Fig. S3 ------- COMMENT: 627a2efc48957a76 4 fZRw7NtG2zoBIL62idcK2whIFb8 Supplementary Table S3 ------- COMMENT: 627a2efc48957a76 7 ZGMZvcYvzp8RGnnUgdqMjmArVL0 Supplementary Fig. S3 ------- COMMENT: 627a2efc48957a76 8 ZGMZvcYvzp8RGnnUgdqMjmArVL0 Supplementary Fig. S3 ------- COMMENT: 627a2efc48957a76 9 ZGMZvcYvzp8RGnnUgdqMjmArVL0 Supplementary Fig. S3 ------- COMMENT: 627a2efc48957a76 10 ZGMZvcYvzp8RGnnUgdqMjmArVL0 Supplementary Fig. S3 ------- COMMENT: 628c4060d26880cf 13 W2bs1lNVjSikB4+HqfXGiZaSqCk (comment: they only show that this is part of a complex that demethylates H3K9 so there is a chance it is not active?) ------- COMMENT: 62ab4a718bc5d4d9 3 ejn0uKmQcgUpnZR6QEnzcVi49Bk Strikingly, however, silencing at the mating type locus was completely abolished in the 22 absence of Caf1 and Mot2, similar to clr4∆ cells, as revealed by the lack of cell growth on 23 5FOA-containing medium and the marked accumulation of ura4+ transcripts (Fig. 1g-h). ------- COMMENT: 62ab4a718bc5d4d9 7 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 62ab4a718bc5d4d9 18 lgFTJrOCdcL86+9phrs9tRJPzkk We also detected intermediate phenotypes for the 8 ccr4∆ mutant, pointing to a partial contribution of this RNA deadenylase (Fig. 1g-h; 9 Supplementary Fig. 1b-d). ------- COMMENT: 62ab4a718bc5d4d9 48 ejn0uKmQcgUpnZR6QEnzcVi49Bk Strikingly, however, silencing at the mating type locus was completely abolished in the 22 absence of Caf1 and Mot2, similar to clr4∆ cells, as revealed by the lack of cell growth on 23 5FOA-containing medium and the marked accumulation of ura4+ transcripts (Fig. 1g-h). ------- COMMENT: 63373b8b600ffce2 2 RWIy41iJSFsFucwi5nAKR9IvqW4 (comment: CHECK protein phosphorylation assayed in vitro) ------- COMMENT: 63373b8b600ffce2 4 RWIy41iJSFsFucwi5nAKR9IvqW4 (comment: CHECK protein phosphorylation assayed in vitro) ------- COMMENT: 63373b8b600ffce2 5 qn1//tNxNgySvt5wNop3gOHtZhA (comment: CHECK Phosphorylation assayed in vitro) ------- COMMENT: 63373b8b600ffce2 6 qn1//tNxNgySvt5wNop3gOHtZhA (comment: CHECK Phosphorylation assayed in vitro) ------- COMMENT: 63373b8b600ffce2 8 qn1//tNxNgySvt5wNop3gOHtZhA (comment: CHECK Phosphorylation assayed in vitro) ------- COMMENT: 63373b8b600ffce2 10 qn1//tNxNgySvt5wNop3gOHtZhA (comment: CHECK Phosphorylation assayed in vitro) ------- COMMENT: 63373b8b600ffce2 12 qn1//tNxNgySvt5wNop3gOHtZhA (comment: CHECK Phosphorylation assayed in vitro) ------- COMMENT: 63373b8b600ffce2 14 IncTYg9LzgNS/0Hjltd1bhyiOPo (comment: CHECK Protein phosphorylation assayed in vitro) ------- COMMENT: 63373b8b600ffce2 16 IncTYg9LzgNS/0Hjltd1bhyiOPo (comment: CHECK Protein phosphorylation assayed in vitro) ------- COMMENT: 63373b8b600ffce2 18 RWIy41iJSFsFucwi5nAKR9IvqW4 (comment: CHECK protein phosphorylation assayed in vitro) ------- COMMENT: 63373b8b600ffce2 33 qn1//tNxNgySvt5wNop3gOHtZhA (comment: CHECK Phosphorylation assayed in vitro) ------- COMMENT: 63373b8b600ffce2 58 RWIy41iJSFsFucwi5nAKR9IvqW4 (comment: CHECK protein phosphorylation assayed in vitro) ------- COMMENT: 63373b8b600ffce2 60 RWIy41iJSFsFucwi5nAKR9IvqW4 (comment: CHECK protein phosphorylation assayed in vitro) ------- COMMENT: 63373b8b600ffce2 62 RWIy41iJSFsFucwi5nAKR9IvqW4 (comment: CHECK protein phosphorylation assayed in vitro) ------- COMMENT: 63373b8b600ffce2 63 RWIy41iJSFsFucwi5nAKR9IvqW4 (comment: CHECK protein phosphorylation assayed in vitro) ------- COMMENT: 63373b8b600ffce2 66 RWIy41iJSFsFucwi5nAKR9IvqW4 (comment: CHECK protein phosphorylation assayed in vitro) ------- COMMENT: 63373b8b600ffce2 67 RWIy41iJSFsFucwi5nAKR9IvqW4 (comment: CHECK protein phosphorylation assayed in vitro) ------- COMMENT: 6339283c028dd2d6 2 R03ci7CVVbze/2PPEvbE6cmuvmY (Fig. 1B). The asp1-STF7 pyrophosphatase mutation resulted in derepression of Pho1 expression, by 23-fold compared to the wild-type control. ------- COMMENT: 6339283c028dd2d6 3 wbdXppKs64moJdW/Nn9Rog3M8yc The STF7 (H686Y) pyrophosphatase domain mutation of asp1 elicits a severe growth defect at all temperatures (Fig. 1A). ------- COMMENT: 6339283c028dd2d6 4 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 6339283c028dd2d6 5 Pp7ifTqkjcEf8sordcFDmHPGXjc The snf22∆ asp1-H397A double mutant grew well on YES agar at all temperatures from 20°C to 37°C (Fig. 2A) ------- COMMENT: 6339283c028dd2d6 6 Of6h9c/+zdwOkAHZN33ic5tyvME snf22∆ cells grew similarly to wild-type cells on YES agar at all temperatures tested (Fig. 2A). ------- COMMENT: 6339283c028dd2d6 7 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 6339283c028dd2d6 8 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 6339283c028dd2d6 9 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 6339283c028dd2d6 10 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 6339283c028dd2d6 11 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 6339283c028dd2d6 12 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 6339283c028dd2d6 13 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 6339283c028dd2d6 14 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: 6339283c028dd2d6 15 uWlYAPleV87uV39aLJMSCUS99fs (i) snf22∆ suppressed the cold-sensitive slow growth phenotype associated with seb1-G476S (Fig. 2A) ------- COMMENT: 6339283c028dd2d6 16 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 6339283c028dd2d6 17 N8bE1Q2Z9u0mwB2BnMWu0VluMUg snf22∆ elicited a sevenfold hyper-repression of Pho1 expression in phosphate-replete cells compared to the wild-type control (Fig. 2B, P value <0.0001). ------- COMMENT: 6339283c028dd2d6 18 RWWxZoCoYl/RSjdMDwATo5kDmq8 The derepression of Pho1 seen in asp1-H397A cells was erased by snf22∆, to the extent that acid phosphatase activity in snf22∆ asp1-H397A cells was the same as that of the wild-type control (Fig. 2B). ------- COMMENT: 6339283c028dd2d6 19 RWWxZoCoYl/RSjdMDwATo5kDmq8 The derepression of Pho1 seen in asp1-H397A cells was erased by snf22∆, to the extent that acid phosphatase activity in snf22∆ asp1-H397A cells was the same as that of the wild-type control (Fig. 2B). ------- COMMENT: 6339283c028dd2d6 20 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6339283c028dd2d6 21 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6339283c028dd2d6 22 +a0waEXXa2UaNPpgIokwF+eC3DE Pho1 expression is therefore derepressed in seb1-G476S cells (Fig. 2B). , 3B ------- COMMENT: 6339283c028dd2d6 23 QWaxn300gK4i1JFHhO1MPQWGOBU Production of full-length interfering prt lncRNA is inhibited in rad24∆ cells (31) concomitant with increased production of pho1 mRNA and greatly increased Pho1 activity (as in Fig. 2B). ------- COMMENT: 6339283c028dd2d6 24 Hkq61v8sq5JO6/yg4lniFRQTkMY Deletion of the Nudix-family pyrophosphatase Aps1 or the Duf89 phosphatase-pyro­ phosphatase also results in the derepression of Pho1 expression (15, 30), an effect that was suppressed by snf22∆ (Fig. 2B). ------- COMMENT: 6339283c028dd2d6 25 Hkq61v8sq5JO6/yg4lniFRQTkMY Deletion of the Nudix-family pyrophosphatase Aps1 or the Duf89 phosphatase-pyro­ phosphatase also results in the derepression of Pho1 expression (15, 30), an effect that was suppressed by snf22∆ (Fig. 2B). ------- COMMENT: 6339283c028dd2d6 26 0YJBH15YVJqKPvTEmTNkOtipJYo and (ii) snf22∆ suppressed the derepression of Pho1 by seb1-G476S (Fig. 2B). ------- COMMENT: 6339283c028dd2d6 27 +/tSzcL5BKlSoVRCLkZ5PXY4R08 Here we constructed a snf22∆ rad24∆ double mutant (Fig. 2A) and found that loss of Snf22 abolished Pho1 derepression by rad24∆ (Fig. 2B). ------- COMMENT: 6339283c028dd2d6 28 ZR8ls9Db3cdxeXJO+JWK+v+DouI Fig. 3A, 4A ------- COMMENT: 6339283c028dd2d6 29 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 6339283c028dd2d6 30 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 6339283c028dd2d6 31 w0qAlugXKzP8B6/jRJXzOkWfgLM Moreover, snf22-(D996A-E997A) reversed the derepression of Pho1 accompanying deletion of erh1 (Fig. 3B), which results from precocious 3′-processing/termination of prt lncRNA synthesis (38). ------- COMMENT: 6339283c028dd2d6 32 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 6339283c028dd2d6 33 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 6339283c028dd2d6 34 w0qAlugXKzP8B6/jRJXzOkWfgLM Moreover, snf22-(D996A-E997A) reversed the derepression of Pho1 accompanying deletion of erh1 (Fig. 3B), which results from precocious 3′-processing/termination of prt lncRNA synthesis (38). ------- COMMENT: 6339283c028dd2d6 35 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 6339283c028dd2d6 36 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 6339283c028dd2d6 37 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 6339283c028dd2d6 38 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 6339283c028dd2d6 39 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 6339283c028dd2d6 40 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 6339283c028dd2d6 41 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 6339283c028dd2d6 42 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 6339283c028dd2d6 43 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 6339283c028dd2d6 44 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 6339283c028dd2d6 45 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 6339283c028dd2d6 46 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 6339283c028dd2d6 47 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 6339283c028dd2d6 48 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 6339283c028dd2d6 49 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 6339283c028dd2d6 50 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 6339283c028dd2d6 51 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 6339283c028dd2d6 52 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 6339283c028dd2d6 53 w/aslf1Nyiuv5myDkPgLkJT1naw Fig. 4C, 6E ------- COMMENT: 6339283c028dd2d6 54 w/aslf1Nyiuv5myDkPgLkJT1naw Fig. 4C, 6E ------- COMMENT: 6339283c028dd2d6 55 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 6339283c028dd2d6 56 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 6339283c028dd2d6 57 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 6339283c028dd2d6 58 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 6339283c028dd2d6 59 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 6339283c028dd2d6 60 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 6339283c028dd2d6 61 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 6339283c028dd2d6 62 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 6339283c028dd2d6 63 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 6339283c028dd2d6 64 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 6339283c028dd2d6 65 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 6339283c028dd2d6 66 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 6339283c028dd2d6 67 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 6339283c028dd2d6 68 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 6339283c028dd2d6 69 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 6339283c028dd2d6 70 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 6339283c028dd2d6 71 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 6339283c028dd2d6 72 BqJfJj50g9a0BggqWa7h/4i1GLo SST-612 in Fig. 6A ------- COMMENT: 6339283c028dd2d6 73 qeVNWcOWzOfS8ah0QzO7PLDnigg Fig. 6A, 6D (STF6) ------- COMMENT: 6339283c028dd2d6 74 JWFuo1rINzhmE3l1DvKWec+ZNYI SST-612 in Fig. 6B ------- COMMENT: 6339283c028dd2d6 75 Sz1sLp9jK7DvD/GYAMw1mY9E+YQ Fig. 6D (STF9) ------- COMMENT: 6339283c028dd2d6 76 xLld+U4XtO1Yq5XJ/vA+lPgihjU Fig. 6D ------- COMMENT: 6339283c028dd2d6 77 /EKJDn1kiFepVVy1Uoj4agCFYLE Fig. 6E ------- COMMENT: 6339283c028dd2d6 78 /EKJDn1kiFepVVy1Uoj4agCFYLE Fig. 6E ------- COMMENT: 6339283c028dd2d6 79 /EKJDn1kiFepVVy1Uoj4agCFYLE Fig. 6E ------- COMMENT: 6339283c028dd2d6 80 xLld+U4XtO1Yq5XJ/vA+lPgihjU Fig. 6D ------- COMMENT: 6339283c028dd2d6 81 xLld+U4XtO1Yq5XJ/vA+lPgihjU Fig. 6D ------- COMMENT: 6339283c028dd2d6 82 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 83 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 84 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 85 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 86 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 87 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 88 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 89 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 90 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 91 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 92 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 93 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 94 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 95 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 96 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 97 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 98 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 99 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 100 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 101 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 102 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 103 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 104 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 105 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 106 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 107 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 108 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 109 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 110 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 111 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 112 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 113 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 114 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 115 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 116 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 117 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 118 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 119 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 120 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 121 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 122 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 123 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 124 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 125 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 126 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 127 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 128 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 129 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 130 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 131 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 132 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 133 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 134 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 135 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 136 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 137 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 138 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 139 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 140 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 141 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 142 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 6339283c028dd2d6 143 24OZrOgdlqsMzgBAIO26tu76ih0 Fig. 12 ------- COMMENT: 6339283c028dd2d6 144 4JQt0wN2TH7w8wc2RC2zx0BwanM Fig. S1A ------- COMMENT: 6339283c028dd2d6 145 AJM3UzZfcNA51z7jraSDCDOL5LY Fig. S1B ------- COMMENT: 6339283c028dd2d6 146 4JQt0wN2TH7w8wc2RC2zx0BwanM Fig. S1A ------- COMMENT: 6339283c028dd2d6 147 AJM3UzZfcNA51z7jraSDCDOL5LY Fig. S1B ------- COMMENT: 6339283c028dd2d6 148 4JQt0wN2TH7w8wc2RC2zx0BwanM Fig. S1A ------- COMMENT: 6339283c028dd2d6 149 AJM3UzZfcNA51z7jraSDCDOL5LY Fig. S1B ------- COMMENT: 6339283c028dd2d6 150 ifrqlcy7G0f4EFipSEbm7b0gMNU Fig. S2A ------- COMMENT: 6339283c028dd2d6 151 ifrqlcy7G0f4EFipSEbm7b0gMNU Fig. S2A ------- COMMENT: 6339283c028dd2d6 152 +Ek/y8FI1jr63JOLTj8F9c2kaEQ Fig. S2B ------- COMMENT: 6339283c028dd2d6 153 +Ek/y8FI1jr63JOLTj8F9c2kaEQ Fig. S2B ------- COMMENT: 6339283c028dd2d6 154 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 155 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 156 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 157 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 158 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 159 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 160 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 161 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 162 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 163 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 164 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 165 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 166 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 167 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 168 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 169 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 170 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 171 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 172 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 173 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 174 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 175 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 176 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 177 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 178 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 179 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 180 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 181 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 182 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 183 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 184 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 185 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 186 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 187 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 188 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 189 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 190 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 191 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 192 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 193 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 194 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 195 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 196 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 197 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 198 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 199 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 200 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 201 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 6339283c028dd2d6 202 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 6339283c028dd2d6 203 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 6339283c028dd2d6 204 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 6339283c028dd2d6 205 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 6339283c028dd2d6 206 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 6339283c028dd2d6 207 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 6339283c028dd2d6 208 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 6339283c028dd2d6 209 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 6356242fe0e335bd 1 VInyPcCkoaLQYeiJVVMuSPubbaQ Fig. 2A; ------- COMMENT: 6356242fe0e335bd 2 VInyPcCkoaLQYeiJVVMuSPubbaQ Fig. 2A; ------- COMMENT: 6356242fe0e335bd 3 VInyPcCkoaLQYeiJVVMuSPubbaQ Fig. 2A; ------- COMMENT: 6356242fe0e335bd 4 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6356242fe0e335bd 5 oSCroffzZs/EEkGjsuS9+4xWuwM This suggested that the inability to promote mitotic Plo1-associated kinase activity in cut12.1 cells was not a simple consequence of an inability to assemble a bipolar spindle,or an inability to commit to mitosis.Rather,the data indicate that Cut12 function was required for full activationof Plo1-associated kinase activity during mitotic commitment. Alspp Fig. 6B ------- COMMENT: 6356242fe0e335bd 6 CnAJlWndAmu1ee0nuZxIU/khcp0 Figs.1A,4C) ------- COMMENT: 6356242fe0e335bd 7 JTtaPg861SPHDbwy/Hqf1Kr5I2k Plo1-associated kinase activity of extracts from arrested cdc2.33 cut12.s11 cells was 2.4-fold(±0.35;n=6)higher than that of the control cdc2.33 cut12+ cells (Fig. 4D). This established that cut12.s11 increased Plo1 activity ininterphase. ------- COMMENT: 6356242fe0e335bd 8 ipNMNAMrfHML7P9et9HGdG1+iQA Kinase assays of these mitotic samples indicated that the cut12.s11 mutation promoted a 1.6 (±0.18; n = 5) in-crease in Plo1-specific activity during mitosis(Fig.4D). ------- COMMENT: 6356242fe0e335bd 9 i3lndki5IpLpKhIZpGNxEFhOq8k Both plo1.ts2 and plo1.ts19 conferred temperature sensitivity for growth on minimal medium ------- COMMENT: 6356242fe0e335bd 10 i3lndki5IpLpKhIZpGNxEFhOq8k Both plo1.ts2 and plo1.ts19 conferred temperature sensitivity for growth on minimal medium ------- COMMENT: 6356242fe0e335bd 11 19LLpsjRPF3MYP1QJGch/Em383E ability to form colonies o nrich medium at 36°C was indistin- guishable from that of wild-typec ells ------- COMMENT: 6356242fe0e335bd 12 19LLpsjRPF3MYP1QJGch/Em383E ability to form colonies o nrich medium at 36°C was indistin- guishable from that of wild-typec ells ------- COMMENT: 6356242fe0e335bd 13 sFw+KJ/82qaCGA8v8K0hiujRcBQ Western blot analysis showed that Plo1 levels in plo1.ts2cellswerenotradicallydifferentfromwildtype, ------- COMMENT: 6356242fe0e335bd 14 0g6odQ1c/sMk1bCHWlpgazRWipQ full-length protein appeared to be largely absent from plo1.ts19 on either minimal or rich medium at either 25°C or 36°C (Fig. 7B) ------- COMMENT: 6356242fe0e335bd 15 0g6odQ1c/sMk1bCHWlpgazRWipQ full-length protein appeared to be largely absent from plo1.ts19 on either minimal or rich medium at either 25°C or 36°C (Fig. 7B) ------- COMMENT: 6356242fe0e335bd 16 18UJzWUkEDFzQgJQy1kT/wg8jsI We concluded that the Plo1-dependent kinase activity of both plo1.ts2 and plo1.ts19 was greatly reduced. ------- COMMENT: 6356242fe0e335bd 17 18UJzWUkEDFzQgJQy1kT/wg8jsI We concluded that the Plo1-dependent kinase activity of both plo1.ts2 and plo1.ts19 was greatly reduced. ------- COMMENT: 6356242fe0e335bd 18 UnsZS2QspI3lBbazucp35cPdObs plo1.ts2 strains entered mitosis but did not form spindles ------- COMMENT: 6356242fe0e335bd 19 UnsZS2QspI3lBbazucp35cPdObs plo1.ts2 strains entered mitosis but did not form spindles ------- COMMENT: 6356242fe0e335bd 20 hOirS4FdTaBcxAYzwoa4itVmZXM Unlike classic “cut” mutants (Hirano et al. 1986), septation did not always follow on from the mi- toticarrest. ------- COMMENT: 6356242fe0e335bd 22 3CiSOX5yPyxU4HcPMn800TDcryI Fig 9A. cdc25.22 and cdc25.22 plo1.ts19 cells, on the other hand, could not form colo- nies on this medium at this temperature, but cdc25.22 cut12.s11 cells could ------- COMMENT: 6356242fe0e335bd 23 UYD2vYl6y3OEgS9UJU+6KezIfxs This established that mutating plo1 in a way that did not affect cell viability compromised the ability of cut12.s11 to suppress cdc25.22. Fig 9A. cdc25.22 and cdc25.22 plo1.ts19 cells, on the other hand, could not form colo- nies on this medium at this temperature, but cdc25.22 cut12.s11 cells could ------- COMMENT: 6356242fe0e335bd 24 a28wfv4Ls7ASIl+86wetseM167c triple cut12.s11 cdc25.22 plo1.ts19 cells were unable to grow (Fig. 9A). ------- COMMENT: 6356242fe0e335bd 25 aMHuTrQ5Sm/Rwa5+8VCZh8K5QkA Whereas single plo1.ts2 and plo1.ts19 mutant and double cut12.s11 cdc25.22 mutant cells all entered mitosis (Fig. 9B,C), the single cdc25.22 mutant and both double cdc25.22 plo1.ts and triple cut12.s11 cdc25.22 plo1.ts mutants did not. ------- COMMENT: 6356242fe0e335bd 26 aMHuTrQ5Sm/Rwa5+8VCZh8K5QkA Whereas single plo1.ts2 and plo1.ts19 mutant and double cut12.s11 cdc25.22 mutant cells all entered mitosis (Fig. 9B,C), the single cdc25.22 mutant and both double cdc25.22 plo1.ts and triple cut12.s11 cdc25.22 plo1.ts mutants did not. ------- COMMENT: 6356242fe0e335bd 27 gvHoU//JalkF/rxAWZajYXSy1Kc Whereas cells in which the expres- sionoftheconstitutivelyactivemutantremainedre- pressed arrested cell cycle progression in interphase, 11% of those in which it had been expressed entered mitosis.This degree of suppression is very similar to the level of suppression of cdc25.22 arising from the pres- ence of the cut12.s11 mutation (Fig. 9B) and established that activation of Plo1 is sufficient to suppress the deficiency in Cdc25 function in cdc25.22 cells. ------- COMMENT: 6356242fe0e335bd 28 /M3XlqntzbHhT6Lgvc46/R5TPUg The extended and more random size of plo1.ts2cellsatdivisionsuggestedthatthismaybethe case. Despite the fact that these cells are able to enter mitosis,theyappeartobedoingsoinalessefficient,or more random manner (Fig. 8B). ------- COMMENT: 6356242fe0e335bd 29 CrSkQYsNURlADhY6v6A+5NauMGA Plo1.K65R,the“kinasedead”mutantprotein,onlyas- sociatedwithmitoticbutnotwithinterphaseSPBs(data not shown). ------- COMMENT: 635d1ba383ac123c 1 Jlk1V51+Ro3/iw8g0EtgXjlKa3U fig 5a ------- COMMENT: 635d1ba383ac123c 2 Jlk1V51+Ro3/iw8g0EtgXjlKa3U fig 5a ------- COMMENT: 635d1ba383ac123c 3 8N5pCuJDvQ4tU27Tb2MYBaI8aOw fig 5b ------- COMMENT: 635d1ba383ac123c 4 GAqy8t8jp/JwHdUGBgIUhMOswPI fig 8a ------- COMMENT: 635d1ba383ac123c 5 GAqy8t8jp/JwHdUGBgIUhMOswPI fig 8a ------- COMMENT: 635d1ba383ac123c 6 VLFHAHo36fgWTUijpY5Ysu7zdLU fig 8c ------- COMMENT: 635d1ba383ac123c 7 VLFHAHo36fgWTUijpY5Ysu7zdLU fig 8c ------- COMMENT: 6378670e7a31c356 1 bvROJGHlLz7k5piWvNvkRDaanVo spore viability same in double mutant as rec12delta alone, and higher than rad50 mutants alone ------- COMMENT: 6378670e7a31c356 2 a813+1LPoyJ987xSs/AUOGftIMg spore viability and DSB clipping in mre11-D65N rad50delta both same as in rad50delta alone ------- COMMENT: 63a35e2ffef1be6f 18 C4ocrsI9ddEVS42b75zsbANpdjg (comment: CHECK broken) ------- COMMENT: 63c808bbf4024255 2 9ygdYY9cvg4vazr1GvIznxI7D2A Fig. 2-S1A ------- COMMENT: 63c808bbf4024255 3 9ygdYY9cvg4vazr1GvIznxI7D2A Fig. 2-S1A ------- COMMENT: 63c808bbf4024255 4 9ygdYY9cvg4vazr1GvIznxI7D2A Fig. 2-S1A ------- COMMENT: 63c808bbf4024255 5 9ygdYY9cvg4vazr1GvIznxI7D2A Fig. 2-S1A ------- COMMENT: 63c808bbf4024255 6 9ygdYY9cvg4vazr1GvIznxI7D2A Fig. 2-S1A ------- COMMENT: 63c808bbf4024255 7 9ygdYY9cvg4vazr1GvIznxI7D2A Fig. 2-S1A ------- COMMENT: 63c808bbf4024255 8 9ygdYY9cvg4vazr1GvIznxI7D2A Fig. 2-S1A ------- COMMENT: 63c808bbf4024255 9 9ygdYY9cvg4vazr1GvIznxI7D2A Fig. 2-S1A ------- COMMENT: 63c808bbf4024255 10 9ygdYY9cvg4vazr1GvIznxI7D2A Fig. 2-S1A ------- COMMENT: 63c808bbf4024255 11 YBJTxOd2PzyyYPvwmVor2k/IiTg This further suggests that Mud1 is not playing the same role in regulating Rtf2 in S. pombe as has been observed for DDI1/2 in human cells. Fig. 2-S1B ------- COMMENT: 63c808bbf4024255 12 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 63c808bbf4024255 13 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 63c808bbf4024255 14 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 63c808bbf4024255 15 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 63c808bbf4024255 16 YGHeVLCAzwpZKpQtx9SLd6H8cQ4 expression of an intron-less rtf1 gene that encodes the same additional 15 amino acids between residues 202–203 (Rtf1 intron2NE) does not provoke increased replication slippage downstream of RTS1 (Figure 4—figure supplement 3), indicating that this protein is indeed dysfunctional. ------- COMMENT: 63c808bbf4024255 17 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 18 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 19 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 20 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 21 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 22 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 23 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 24 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 25 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 26 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 27 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 28 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 29 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 30 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 31 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 32 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124 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 125 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 126 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 127 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 128 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 129 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 130 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 131 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 132 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 133 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 134 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 135 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 136 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 137 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 138 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 139 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 140 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 141 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 142 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 143 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 144 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63c808bbf4024255 145 DpsfJpRxrdrEbADi4GJjTjx50AE Supplementary file 1 ------- COMMENT: 63cd6d753b3b6617 1 Wfk3FHLQqxO8KDHIgS2fHjyjJmM (comment: CONDITION 100 ug/mL MPA) ------- COMMENT: 63cd6d753b3b6617 2 qrBmlwswV3EPLTr1bgCryNs08nA (comment: CONDITION EMM -U agar plates, supplemented with 6AU concentration ranging from 3.6 to 150 ug/mL) ------- COMMENT: 63cd6d753b3b6617 7 srmrH/iA0mn7E0wViuWTNF15elI (comment: CHECK at tgp1 promoter) ------- COMMENT: 63cd6d753b3b6617 9 vRHemxl7Pfh5L90FO0qUAGT6kaY (comment: at lncRNAs upstream of PHO regulon genes (nc-tgp1, nc-pho1, prt1)) ------- COMMENT: 63cd6d753b3b6617 10 PAjpMX3Ro8ed1Y1a0GDwWileK8Q (comment: also assayed genome-wide) ------- COMMENT: 63cd6d753b3b6617 11 PAjpMX3Ro8ed1Y1a0GDwWileK8Q (comment: also assayed genome-wide) ------- COMMENT: 63cd6d753b3b6617 12 rvAminmC5a8W7M6jPl5k08cu5zY (comment: at different lncRNA polyadenylation sites) ------- COMMENT: 63f563e7fdecebe3 1 +4Skbr6M2P2J40swV0D4mBvwe1w The requirement for Pir2 in mediating the repressive effects of lncRNAs is a highly significant finding. ------- COMMENT: 63f563e7fdecebe3 2 XR85Tc6HDCNG0t/S5m4BQG+XZ9o Loss of the MTREC subunit Red1 resulted in the accumulation of longer readthrough transcripts (referred to as prt-L and nam1-L) (Fig. 1a), as was also observed in mmi1Δ and rrp6Δ cells (Fig. 1a and Supplementary Fig. 1a)6,7,11. ------- COMMENT: 63f563e7fdecebe3 3 XR85Tc6HDCNG0t/S5m4BQG+XZ9o Loss of the MTREC subunit Red1 resulted in the accumulation of longer readthrough transcripts (referred to as prt-L and nam1-L) (Fig. 1a), as was also observed in mmi1Δ and rrp6Δ cells (Fig. 1a and Supplementary Fig. 1a)6,7,11. ------- COMMENT: 63f563e7fdecebe3 4 HHgxn8WtXMbioCBH+RLMx7EPsnY as was also observed in mmi1Δ and rrp6Δ cells (Fig. 1a and Supplementary Fig. 1a) ------- COMMENT: 63f563e7fdecebe3 5 HHgxn8WtXMbioCBH+RLMx7EPsnY as was also observed in mmi1Δ and rrp6Δ cells (Fig. 1a and Supplementary Fig. 1a) ------- COMMENT: 63f563e7fdecebe3 6 HHgxn8WtXMbioCBH+RLMx7EPsnY as was also observed in mmi1Δ and rrp6Δ cells (Fig. 1a and Supplementary Fig. 1a) ------- COMMENT: 63f563e7fdecebe3 7 HHgxn8WtXMbioCBH+RLMx7EPsnY as was also observed in mmi1Δ and rrp6Δ cells (Fig. 1a and Supplementary Fig. 1a) ------- COMMENT: 63f563e7fdecebe3 8 6cxcJIZqxc570FcxJlOvdRbW81c Surprisingly, pir2-1 showed a drastic upregulation of pho1 and byr2 genes as compared to wild- type (WT) (Fig. 1b), ------- COMMENT: 63f563e7fdecebe3 9 zPZMdwsFZZrmN+ZrrGXdMgg5TsA (Fig. 1b), similar to the effect observed upon deletion of the lncRNA (Supplementary Fig. 1b)6,7,11. ------- COMMENT: 63f563e7fdecebe3 10 zPZMdwsFZZrmN+ZrrGXdMgg5TsA (Fig. 1b), similar to the effect observed upon deletion of the lncRNA (Supplementary Fig. 1b)6,7,11. ------- COMMENT: 63f563e7fdecebe3 11 842JK/jWc4Zxm/rIAG6XQ6tZkUI Chromatin immuno- precipitation followed by sequencing (ChIP-seq) confirmed Pir2 enrichment at lncRNAs, including prt and nam1 (Fig. 1c and Supplementary Fig. 1c) ------- COMMENT: 63f563e7fdecebe3 12 ssp7Q4OC3RlHVTkve0wIHFQIhEE Moreover, RNA immunoprecipitation sequencing analysis (RIP-seq) showed that Pir2 binds to the lncRNAs (Fig. 1d and Supplementary Fig. 1d) ------- COMMENT: 63f563e7fdecebe3 14 IyFSeCGiUq0X3Nz22EWzpvvZ+Y8 Supporting the function of Pir2 and lncRNA in the same pathway, we found no additive effect on pho1 expression in the pir2-1 prtΔ double mutant when compared to the effect in the single mutants (Fig. 1e). ------- COMMENT: 63f563e7fdecebe3 15 ByK3UrBqRD8e4M63SQi48+XKhaI We asked if Pir2 is also required for the repression of byr2 that is observed upon the accumulation of nam1 lncRNA in cells lacking Rrp6. Since byr2 is required for meiotic induction, cells lacking Rrp6 are defective in sporulation (Fig. 1f)11. ------- COMMENT: 63f563e7fdecebe3 17 M6eYZbkMDXV1YgdHNfMOMltT9sM Remarkably, entry into meiosis and sporulation efficiency were restored in pir2-1 rrp6Δ cells (Fig. 1f). ------- COMMENT: 63f563e7fdecebe3 19 QsRL5733Jq6WyLvIJndlJGsQ59A The lncRNA-mediated repression of pho1 was impaired in cbc1-1 cells (Supplementary Fig. 1e), ------- COMMENT: 63f563e7fdecebe3 20 gvT3FAJ7DmXAZE2d/AoCmfT2kng The lncRNA-mediated repression of pho1 was impaired in cbc1-1 cells (Supplementary Fig. 1e), sug- ------- COMMENT: 63f563e7fdecebe3 21 Uztko47ZMgzB5DgoLcrAp48f5pM A significant increase in the level of both pho1 and byr2 mRNAs in cwf10-1 as compared to WT confirmed that the splicing machinery indeed affects the expression of genes repressed by lncRNAs (Fig. 2c) ------- COMMENT: 63f563e7fdecebe3 22 6zxa7WVRpoCbNa67LgcyWwzESk4 Importantly, cwf10-1 rescued the sporulation defect observed in rrp6Δ caused by the silencing of the byr2 gene by nam1 lncRNA (Fig. 2e), similar to pir2-1 (Fig. 1f). ------- COMMENT: 63f563e7fdecebe3 23 uR3guD/X95Ubmr6l7IGx+cNRyXQ These results confirm the biological significance of Pir2 association with splicing machinery and show that these factors collaborate to promote gene repression by lncRNAs. ------- COMMENT: 63f563e7fdecebe3 24 RqukfYZjAE2Dd1MGIDkVZn1WljE Remarkably, strains carrying splice site mutations showed significant upregulation of the pho1 transcript (Fig. 3c), similar to the effect observed in pir2-1, cwf10- 1 and prtΔ (Figs. 1b, 2c and Supplementary Fig. 1b) ------- COMMENT: 63f563e7fdecebe3 25 RqukfYZjAE2Dd1MGIDkVZn1WljE Remarkably, strains carrying splice site mutations showed significant upregulation of the pho1 transcript (Fig. 3c), similar to the effect observed in pir2-1, cwf10- 1 and prtΔ (Figs. 1b, 2c and Supplementary Fig. 1b) ------- COMMENT: 63f563e7fdecebe3 26 gIIhsNWVp3XBDXYVo8eO9iF64kM Co-IP analysis showed Pir2 associates with the Hrr1 subunit of RDRC (Fig. 4a). ------- COMMENT: 63f563e7fdecebe3 27 EJhzZBLCeDvd4NpXzeWnbQIY5x8 Interestingly, this interaction was impaired in the cwf10- 1 mutant, indicating that splicing factors are required for asso- ciation of Pir2 with Hrr1 (Fig. 4b) ------- COMMENT: 63f563e7fdecebe3 28 bkV5IKx6PQrbdQarBIwfvqs3I+g We then analyzed the role of Pir2 in siRNA production in cells lacking Rrp6, which show accumulation of lncRNAs and robust repression of their target loci. We found that siRNAs, which ranged in size from 20–24 nt and mapped to lncRNAs targeting pho1 and byr2, were abolished in both pir2-1 and cwf10-1 mutant backgrounds (Fig. 4c and Supplementary Fig. 4a, b). ------- COMMENT: 63f563e7fdecebe3 29 CNBOUehB84/Nw8dUhmAxHWhUxqg We then analyzed the role of Pir2 in siRNA production in cells lacking Rrp6, which show accumulation of lncRNAs and robust repression of their target loci. We found that siRNAs, which ranged in size from 20–24 nt and mapped to lncRNAs targeting pho1 and byr2, were abolished in both pir2-1 and cwf10-1 mutant backgrounds (Fig. 4c and Supplementary Fig. 4a, b). Pir2 was also required for siRNA production at Tf2 elements, pericentromeric repeats, and other loci (Fig. 4c, Supplementary Fig. 5 and Supplementary Data 2). ------- COMMENT: 63f563e7fdecebe3 30 nnpeMy1uEmw/kLhZtHQ7bYKizOo We next wondered whether cryptic introns are required for Pir2- dependent generation of siRNAs. Mutations of the pho1 cryptic intron splice sites in rrp6Δ cells abolished the production of siRNAs mapping to the entire prt lncRNA, including the region upstream of pho1 (Fig. 4d). This result suggests that the cryptic intron acts as part of the prt lncRNA to engage RNAi machinery. Importantly, siRNAs mapping to other loci were not affected (Fig. 4d and Supplementary Fig. 5), ------- COMMENT: 63f563e7fdecebe3 31 E87UAllMnmutdUIXgKNFlNXRADc Cells lacking Ago1 showed a considerable increase in pho1 transcript levels as determined by northern blot analysis (Fig. 4e), but the observed effect was weaker than in pir2-1 or cwf10-1, suggesting that additional factors likely cooperate with Pir2- splicing machinery. ------- COMMENT: 63f563e7fdecebe3 32 XaxgpVHzkIbcmprQPmR2J2LUlfA Clr3 and the Pob3 subunit of FACT asso- ciated with Pir2 in our biochemical analyses (Fig. 5a, b). ------- COMMENT: 63f563e7fdecebe3 33 XaxgpVHzkIbcmprQPmR2J2LUlfA Clr3 and the Pob3 subunit of FACT asso- ciated with Pir2 in our biochemical analyses (Fig. 5a, b). ------- COMMENT: 63f563e7fdecebe3 34 JB8bqqW6tRsDMrNTq685mEnJPzc The loss of Clr3 or Pob3 caused an increase in pho1 transcript levels, consistent with their involvement in repression by lncRNA8,38, but the extent of upregulation was less than in pir2-1 (Fig. 5c). ------- COMMENT: 63f563e7fdecebe3 35 JB8bqqW6tRsDMrNTq685mEnJPzc The loss of Clr3 or Pob3 caused an increase in pho1 transcript levels, consistent with their involvement in repression by lncRNA8,38, but the extent of upregulation was less than in pir2-1 (Fig. 5c). ------- COMMENT: 63f563e7fdecebe3 36 JBfHtudgiMvoMde/YLvara615AI However, the ago1Δ clr3Δ double mutant showed cumulative de-repression of pho1 (Fig. 5d). ------- COMMENT: 63f563e7fdecebe3 37 EbPUrA1MXIv4+caJekzrs0z9tFQ Moreover, quantitative ChIP analyses showed enrichment of Clr3 and Pob3 at prt-pho1 in WT cells and a reduced localization in pir2-1 cells (Fig. 5e). ------- COMMENT: 63f563e7fdecebe3 38 EbPUrA1MXIv4+caJekzrs0z9tFQ Moreover, quantitative ChIP analyses showed enrichment of Clr3 and Pob3 at prt-pho1 in WT cells and a reduced localization in pir2-1 cells (Fig. 5e). ------- COMMENT: 640f264024eab7f9 20 BU9yxTKAPfHPgPg8iggsPzme2YM (comment: total alpha tubulin level reduced but not known whether from nda3 or atb2 or both) ------- COMMENT: 640f264024eab7f9 24 nlesXNg95wbKh+KAaNKXzL3lF4g (comment: total alpha tubulin level reduced but not known whether from nda2 or atb2 or both) ------- COMMENT: 6451b9d7a7729c14 5 gaPBFZSThEyaA2VO3616N5uQCr4 (comment: endosomal localization requires F-actin -assayed using latrunculin A) ------- COMMENT: 6464e04ca419e15b 1 KUQhe7O3jmzOdQbP97B5PTA+nX4 First, we determined whether cfr1delta sensitivity to high KCl concentrations was due to an inability to grow under ionic or osmotic stress. To do so, we analyzed cfr11 sensitivity to several potassium salts at different concentrations, which depended on the concentration that inhibited the growth of the WT, and to sorbitol (Figure 1A). cfr11 exhibited growth defects in the presence of 0.6 M potassium chloride (KCl), 0.6 M potassium nitrate (KNO3), and 0.02 M potassium acetate (CH3 CO2 K). cfr11 exhibited growth defects in the presence of 0.6 M potassium chloride (KCl), 0.6 M potassium nitrate (KNO3), and 0.02 M potassium acetate (CH3 CO2 K). |Interestingly, deleting cch1+ suppressed cfr1delta sensitivity to CaCl2 (Supplementary Figure 4). ------- COMMENT: 6464e04ca419e15b 2 cnW1/ma5KCQWG6n8+i9Bh4Jow7U In contrast, the mutant grew well in the presence of 1.2 M sorbitol, a medium with similar osmolarity to 0.6 M KCl. ------- COMMENT: 6464e04ca419e15b 3 7d2SuuOkTNf6lb3Dyxpzw+VJJ2A we analyzed the growth of prototrophic WT and bch1delta strains on YES plates with 0.6 M KCl. We found that the mutant was sensitive under these conditions (Figure 1C). | figure 5C ------- COMMENT: 6464e04ca419e15b 4 IPYaHKZMy7MS5fbAYHMacR0Pwsk The results showed that this content was significantly higher in the exomer mutant than in the WT (Figure 1D), data that confirmed that exomer plays a role in the regulation of K+ homeostasis. ------- COMMENT: 6464e04ca419e15b 5 IPYaHKZMy7MS5fbAYHMacR0Pwsk The results showed that this content was significantly higher in the exomer mutant than in the WT (Figure 1D), data that confirmed that exomer plays a role in the regulation of K+ homeostasis. ------- COMMENT: 6464e04ca419e15b 6 5DmBQYC2uK5QWjfbvf22QZBDXeU Nonetheless, we compared the growth of trk1delta and trk2delta with that of cfr1delta in the presence of high K+ concentrations. Additionally, we constructed double and triple mutants to determine whether cfr1+ acts in the same functional pathways as trk1+ and/or trk2+. The results showed that while cfr1delta was sensitive to potassium salts, neither trk1delta, trk2delta nor trk1delta trk2delta exhibited sensitivity (Figure 2A). ------- COMMENT: 6464e04ca419e15b 7 DweHljmH6/P8AniI8KFP6UnPteo Nonetheless, we compared the growth of trk1delta and trk2delta with that of cfr1delta in the presence of high K+ concentrations. Additionally, we constructed double and triple mutants to determine whether cfr1+ acts in the same functional pathways as trk1+ and/or trk2+. The results showed that while cfr11 was sensitive to potassium salts, neither trk11, trk21 nor trk11 trk21 (denoted by trk11 in the figure) exhibited sensitivity (Figure 2A). ------- COMMENT: 6464e04ca419e15b 8 lrF35XtrAuEzKRCev5sQEIR9xXc Nonetheless, we compared the growth of trk1delta and trk2delta with that of cfr1delta in the presence of high K+ concentrations. Additionally, we constructed double and triple mutants to determine whether cfr1+ acts in the same functional pathways as trk1+ and/or trk2+. The results showed that while cfr11 was sensitive to potassium salts, neither trk1delta, trk2delta nor trk1delta trk2delta exhibited sensitivity (Figure 2A). ------- COMMENT: 6464e04ca419e15b 9 b1q+d+3aHA4GYJD+c/vnDilbUFA Regarding double mutants, trk1delta cfr1delta was more sensitive than cfr1delta, indicating that both genes cooperated and acted in parallel rather than in linear pathways related to K+ sensitivity. ------- COMMENT: 6464e04ca419e15b 10 b1q+d+3aHA4GYJD+c/vnDilbUFA Regarding double mutants, trk1delta cfr1delta was more sensitive than cfr1delta, indicating that both genes cooperated and acted in parallel rather than in linear pathways related to K+ sensitivity. ------- COMMENT: 6464e04ca419e15b 11 QGTKrWmL8E29gdl2+hr8yw/FjG0 The phenotype of trk1delta trk2delta cfr1delta was similar to that of trk2delta cfr1delta. ------- COMMENT: 6464e04ca419e15b 12 TVHqNH+eADDm42EU7QQ2sL3DgcM GFP-Trk1 localized at the cell surface of the cell growing sites (cell poles and equator) in both, WT and cfr11 cells (Figure 2B), confirming proper Trk1 sorting in the absence of exomer. (combined with existing knowledge) ------- COMMENT: 6464e04ca419e15b 13 TVHqNH+eADDm42EU7QQ2sL3DgcM GFP-Trk1 localized at the cell surface of the cell growing sites (cell poles and equator) in both, WT and cfr11 cells (Figure 2B), confirming proper Trk1 sorting in the absence of exomer. (combined with existing knowledge) ------- COMMENT: 6464e04ca419e15b 14 XpvBJ/wBvMSi2pivDGyhRbNv+ZI GFP-Trk1 localized at the cell surface of the cell growing sites (cell poles and equator) in both, WT and cfr11 cells (Figure 2B), confirming proper Trk1 sorting in the absence of exomer. ------- COMMENT: 6464e04ca419e15b 15 2m7lOl1Q34gW61RsSsCjGGdCNo4 We found that neither osr2delta nor kha1delta was sensitive to K+ salts and that the phenotype of the corresponding double mutant was similar to that of the single cfr11 mutant (Figure 2D). ------- COMMENT: 6464e04ca419e15b 16 F9DekspMPt0RqP1qgC1BLQqIqiw We found that neither osr2delta nor kha1delta was sensitive to K+ salts and that the phenotype of the corresponding double mutant was similar to that of the single cfr1delta mutant (Figure 2D). ------- COMMENT: 6464e04ca419e15b 17 rWneuwUqa0Mg9aT0/6G+oejGITU We found that neither osr2delta nor kha1delta was sensitive to K+ salts and that the phenotype of the corresponding double mutant was similar to that of the single cfr1delta mutant (Figure 2D) ------- COMMENT: 6464e04ca419e15b 18 b3xnwt2ZyecdqHEKdVJfXXcH6cs cfr1delta was partially sensitive to hygromycin B and deleting cfr1+ enhanced the sensitivity of trk1delta, trk2delta, and trk1delta trk2delta strains (Figure 3C). ------- COMMENT: 6464e04ca419e15b 19 b3xnwt2ZyecdqHEKdVJfXXcH6cs cfr1delta was partially sensitive to hygromycin B and deleting cfr1+ enhanced the sensitivity of trk1delta, trk2delta, and trk1delta trk2delta strains (Figure 3C). ------- COMMENT: 6464e04ca419e15b 20 b3xnwt2ZyecdqHEKdVJfXXcH6cs cfr1delta was partially sensitive to hygromycin B and deleting cfr1+ enhanced the sensitivity of trk1delta, trk2delta, and trk1delta trk2delta strains (Figure 3C). ------- COMMENT: 6464e04ca419e15b 21 b3xnwt2ZyecdqHEKdVJfXXcH6cs cfr1delta was partially sensitive to hygromycin B and deleting cfr1+ enhanced the sensitivity of trk1delta, trk2delta, and trk1delta trk2delta strains (Figure 3C). ------- COMMENT: 6464e04ca419e15b 22 p3hVqgM62ak+mdo/V+PRCxSzQOw (Figure 3C) ------- COMMENT: 6464e04ca419e15b 23 8dCHak90INOlKMYmQsOMnZI54VY The results showed that trk1delta was partially sensitive to 40 mM NaCl and sensitive to 100 mM CaCl2 (Figure 3D) ------- COMMENT: 6464e04ca419e15b 24 QRBbYgJQHM3UbiU4T8OwbS3FzV4 trk1delta trkdelta was the most sensitive of all the strains. ( (Figure 3D) ------- COMMENT: 6464e04ca419e15b 25 8dCHak90INOlKMYmQsOMnZI54VY The results showed that trk1delta was partially sensitive to 40 mM NaCl and sensitive to 100 mM CaCl2 (Figure 3D) ------- COMMENT: 6464e04ca419e15b 26 k83KByTNYLoubOHyFo9Er0sp8Z8 cfr1delta was only sensitive to very high Na+ and Ca2+ concentrations (lower panels in Figure 3D) ------- COMMENT: 6464e04ca419e15b 27 k83KByTNYLoubOHyFo9Er0sp8Z8 cfr1delta was only sensitive to very high Na+ and Ca2+ concentrations (lower panels in Figure 3D) ------- COMMENT: 6464e04ca419e15b 28 g8kCWhXFFtmo4EhMLqbt1WEvN/Q We found that the simultaneous deletion of exomer components and cta3+ led to sensitivity to low concentrations of KCl and KNO3 (Figure 4a) ------- COMMENT: 6464e04ca419e15b 29 g8kCWhXFFtmo4EhMLqbt1WEvN/Q We found that the simultaneous deletion of exomer components and cta3+ led to sensitivity to low concentrations of KCl and KNO3 (Figure 4a) ------- COMMENT: 6464e04ca419e15b 30 AGKI80nIez4OU3/tWpzsJqrkd04 while cta31 was only sensitive to 0.6 M KCl in the tup11delta tup12delta background (Figure 4a) ------- COMMENT: 6464e04ca419e15b 31 bM37xj3W3b1bpGYXCoynrqREhgg Since Cta3 was originally described as an ATP-dependent Ca2+ pump (Ghislain et al., 1990; Halachmi et al., 1992), we also analyzed growth on CaCl2 plates and found that cta3delta was only sensitive to high calcium concentrations, and only in the absence of the Tup regulators. ------- COMMENT: 6464e04ca419e15b 32 bM37xj3W3b1bpGYXCoynrqREhgg Since Cta3 was originally described as an ATP-dependent Ca2+ pump (Ghislain et al., 1990; Halachmi et al., 1992), we also analyzed growth on CaCl2 plates and found that cta3delta was only sensitive to high calcium concentrations, and only in the absence of the Tup regulators. ------- COMMENT: 6464e04ca419e15b 33 t1W501fXgCu7gt5wwnOxypgJHIc Under these conditions, cta31 bch11 was sensitive to lower calcium concentrations. ------- COMMENT: 6464e04ca419e15b 34 O1EwlSIG/hUODZzWFjEbOeDQuRU The result was that overexpression of the extrusion pump alleviated the growth of cfr11 in the presence of high K+ concentrations (Figure 4C). ------- COMMENT: 6464e04ca419e15b 35 O1EwlSIG/hUODZzWFjEbOeDQuRU The result was that overexpression of the extrusion pump alleviated the growth of cfr11 in the presence of high K+ concentrations (Figure 4C). ------- COMMENT: 6464e04ca419e15b 36 crJnLiqCzLtQB05KSebJ81WF1nI Regarding protein localization, microscopy observation showed that 1 h after the addition of KCl most Cta3-GFP accumulated at the cell periphery of the sites of polarized growth (cell poles and equator), and that the intensity of the fluorescence in this location was similar in WT and mutant cells (Figure 4E and Supplementary Figure 2). ------- COMMENT: 6464e04ca419e15b 37 crJnLiqCzLtQB05KSebJ81WF1nI Regarding protein localization, microscopy observation showed that 1 h after the addition of KCl most Cta3-GFP accumulated at the cell periphery of the sites of polarized growth (cell poles and equator), and that the intensity of the fluorescence in this location was similar in WT and mutant cells (Figure 4E and Supplementary Figure 2). ------- COMMENT: 6464e04ca419e15b 38 /FEPaJ31FwgO5KZYssxGlWBeNu0 in the WT about 10% of the cells exhibited asymmetrical and subapical Cta3 distribution, while this percentage was over 50% in the mutant (lower right panel in Figure 4F). ------- COMMENT: 6464e04ca419e15b 39 +NhIvvRMMGL0+HyL3eK538Yxs74 A close observation of the results indicated that under basal conditions (0′), the WT amount of GFP was significantly greater in cfr11 than in the WT, which suggested increased intracellular Ca2 + . | However, in cfr11 cells the amount of Ca2+ continued increasing for another 15 min, and reached a peak that was more than three-fold the value of the baseline (Figure 5B). The maximum Ca2+ level in cfr11 was 67% higher than in the WT | To understand whether the cytoplasmic calcium increase was produced by influx from the exterior or by movements from internal reservoirs, we analyzed the Ca2+ level in the presence of EGTA. We found that the presence of the chelator in the medium completely blocked the increase in Ca2+ levels in both the WT and the mutant cells (Figure 5B). In summary, we concluded that Cfr1 modulated the cellular Ca2+ response to KCl stress by regulating some aspect of Ca2 + influx. ------- COMMENT: 6464e04ca419e15b 40 3+pb6ewPQFkks028mbvPfu7keLs (Figure 5C)) ------- COMMENT: 6464e04ca419e15b 41 TxHq4b+49EvuxWZpdrMxFj1Nkws figure 5C ------- COMMENT: 6464e04ca419e15b 42 TxHq4b+49EvuxWZpdrMxFj1Nkws figure 5C ------- COMMENT: 6464e04ca419e15b 44 f2lBVBH1Um1T9HHWLL0zlhZKa9o (Figure 5.) ------- COMMENT: 6464e04ca419e15b 45 f2lBVBH1Um1T9HHWLL0zlhZKa9o (Figure 5.) ------- COMMENT: 6464e04ca419e15b 46 f2lBVBH1Um1T9HHWLL0zlhZKa9o (Figure 5.) ------- COMMENT: 6464e04ca419e15b 47 WXgd8EAIVIRxfScSlePeWyRLFzs We found that Pkd2-GFP was at the cell surface, with strong accumulation at the septal area in both strains (Figure 5D). Quantitative analyses did not detect significant differences between the strains regarding the distribution and intensity of Pkd2-GFP fluorescence at the cell surface, neither under basal conditions nor in KCl (Supplementary Figure 3). ------- COMMENT: 6464e04ca419e15b 48 UwdPC2j4LpklI227sglceIhl12I In addition, there was intracellular Pkd2-GFP fluorescence. All cells exhibited a faint signal that corresponded to the vacuoles, which in S. pombe are small and numerous, and some cells exhibited at least one bright intracellular dot (denoted by an arrow in Figure 5D). In the WT, less than 20% of the cells exhibited bright intracellular dots, while this number was over 40% in cfr11 (Figure 5F). This difference was evident in the absence of KCl, showing that it was produced by a lack of exomer. Moreover, the dots were brighter in the mutant than in the control (Figure 5G). Western blotting showed that stronger fluorescence was not due to higher levels of the protein (Figure 5H). Quantitative colocalization analyses showed that most of these dots corresponded to the TGN and that fewer dots corresponded to the PVE (Figure 5I and Supplementary Figure 3). ------- COMMENT: 6464e04ca419e15b 49 JoBNZ/XktKQ4j/PMbwV2Z3+ZtcE Exposure to KCl resulted in a small but significant change in Pkd2 distribution, with a reduction in the number of dots that corresponded to the TGN and an increase in the number of dots that corresponded to the PVE. Pkd2 accumulation in the PVE in the presence of KCl was stronger in cfr11 than in the WT. Thus, the effect of potassium in Pkd2 intracellular distribution was enhanced by exomer deletion. ------- COMMENT: 6464e04ca419e15b 50 EvQd0Sgzhtai46tZkOLPcUumsCE As shown in Supplementary Figure 4, their deletion neither produced sensitivity to KCl nor enhanced the sensitivity of bch1delta ------- COMMENT: 6464e04ca419e15b 51 EvQd0Sgzhtai46tZkOLPcUumsCE As shown in Supplementary Figure 4, their deletion neither produced sensitivity to KCl nor enhanced the sensitivity of bch1delta ------- COMMENT: 6464e04ca419e15b 53 Hm6B+MDD24Z88Dt2DG6ByfkTHPE Conversely, double mutants were more sensitive to CaCl2 than single mutants. (Figure S4) ------- COMMENT: 6464e04ca419e15b 54 Hm6B+MDD24Z88Dt2DG6ByfkTHPE Conversely, double mutants were more sensitive to CaCl2 than single mutants. (Figure S4) ------- COMMENT: 6464e04ca419e15b 55 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 6464e04ca419e15b 56 MYZUnyZnyGJYT3wy8ccvjwG6zvA We also analyzed the relationship between exomer and the calcium channels Cch1 and Yam8 (Ma et al., 2011). cch11 was sensitive to low KCl concentrations, and both cch11 cfr11 and yam81 cfr11 were more sensitive than any of the single mutants. ------- COMMENT: 6464e04ca419e15b 57 XZuT9JLGn0NQSSPWsU/Yv8V73JI We also analyzed the relationship between exomer and the calcium channels Cch1 and Yam8 (Ma et al., 2011). cch1delta was sensitive to low KCl concentrations, and both cch1delta cfr1delta and yam8delta cfr1delta were more sensitive than any of the single mutants. ------- COMMENT: 6464e04ca419e15b 58 XZuT9JLGn0NQSSPWsU/Yv8V73JI We also analyzed the relationship between exomer and the calcium channels Cch1 and Yam8 (Ma et al., 2011). cch1delta was sensitive to low KCl concentrations, and both cch1delta cfr1delta and yam8delta cfr1delta were more sensitive than any of the single mutants. ------- COMMENT: 6464e04ca419e15b 60 NK7pLH4Y95QtnWzp4a+FIF1cTuI Interestingly, deleting cch1+ suppressed cfr1delta sensitivity to CaCl2 (Supplementary Figure 4). ------- COMMENT: 6464e04ca419e15b 61 CuVvg7NCplDdv4SPRhpW0YZoJcA Taken together, these experiments showed that calcium homeostasis was altered in exomer mutants, that small defects in the transport of Pkd2 might contribute to this alteration, and that Cch1 facilitates a calcium import that is deleterious for exomer mutants. ------- COMMENT: 6464e04ca419e15b 62 CuVvg7NCplDdv4SPRhpW0YZoJcA Taken together, these experiments showed that calcium homeostasis was altered in exomer mutants, that small defects in the transport of Pkd2 might contribute to this alteration, and that Cch1 facilitates a calcium import that is deleterious for exomer mutants. ------- COMMENT: 6464e04ca419e15b 63 AxrIgGIvkJkhkZPf4I9+NeJUjaU pmr1delta was more sensitive to KCl than cfr1delta, and the double mutants were the most sensitive (Supplementary Figure 4). ------- COMMENT: 6464e04ca419e15b 64 NK7pLH4Y95QtnWzp4a+FIF1cTuI Interestingly, deleting cch1+ suppressed cfr1delta sensitivity to CaCl2 (Supplementary Figure 4). ------- COMMENT: 6464e04ca419e15b 65 AxrIgGIvkJkhkZPf4I9+NeJUjaU pmr1delta was more sensitive to KCl than cfr1delta, and the double mutants were the most sensitive (Supplementary Figure 4). ------- COMMENT: 6464e04ca419e15b 66 8tOc0zpvYLerBI0U0xIbOBAQuYs (Supplementary Figure 4). ------- COMMENT: 6464e04ca419e15b 68 NK7pLH4Y95QtnWzp4a+FIF1cTuI Interestingly, deleting cch1+ suppressed cfr1delta sensitivity to CaCl2 (Supplementary Figure 4). ------- COMMENT: 6464e04ca419e15b 69 XmAIxjEHo3B32vuav6Iqs/6uUBY pmr1delta was sensitive to 100 mM CaCl2 while pmr1delta cfr1delta was not (Supplementary Figure 4) ------- COMMENT: 6464e04ca419e15b 70 S5IidBhZrUK74qhH4w2RG4Hq7YQ pmr11 was sensitive to 100 mM CaCl2 while pmr11 cfr11 was not (Supplementary Figure 4) ------- COMMENT: 6464e04ca419e15b 71 m4Mr9E5WWwho2Lgub0Q8Mvhj13o erg51 exhibited a sensitivity to K+ salts and hygromycin B, that was milder than that of exomer mutants, was slightly more sensitive to CaCl2, and grew well on sorbitol (Figure 7A) ------- COMMENT: 6464e04ca419e15b 72 m4Mr9E5WWwho2Lgub0Q8Mvhj13o erg51 exhibited a sensitivity to K+ salts and hygromycin B, that was milder than that of exomer mutants, was slightly more sensitive to CaCl2, and grew well on sorbitol (Figure 7A) ------- COMMENT: 6464e04ca419e15b 73 m4Mr9E5WWwho2Lgub0Q8Mvhj13o erg51 exhibited a sensitivity to K+ salts and hygromycin B, that was milder than that of exomer mutants, was slightly more sensitive to CaCl2, and grew well on sorbitol (Figure 7A) ------- COMMENT: 6464e04ca419e15b 74 m4Mr9E5WWwho2Lgub0Q8Mvhj13o erg51 exhibited a sensitivity to K+ salts and hygromycin B, that was milder than that of exomer mutants, was slightly more sensitive to CaCl2, and grew well on sorbitol (Figure 7A) ------- COMMENT: 6464e04ca419e15b 75 6A2V+Trr82p0VIaj5bieh0ZlL9s (Figure 7A). erg51 bch11 was more sensitive than the single mutants ------- COMMENT: 6464e04ca419e15b 76 TxHq4b+49EvuxWZpdrMxFj1Nkws figure 5C ------- COMMENT: 6464e04ca419e15b 77 ukM6oRSrIRhZ7JBxsDSAsCoxwK0 We found that cfr1delta enhanced its3-1 thermosensitivity, and that its3-1 enhanced cfr11 sensitivity to 0.6 M KCl, which showed genetic interaction (Figure 7D). ------- COMMENT: 6464e04ca419e15b 78 y6F/nd0QmSstQYi9U+ccltTFYCM We found that cfr11 enhanced its3-1 thermosensitivity, and that its3-1 enhanced cfr11 sensitivity to 0.6 M KCl, which showed genetic interaction (Figure 7D). ------- COMMENT: 6464e04ca419e15b 79 W4XhfVgZHa8FifTR+xXd/XBrPfs In summary, in the absence of exomer and in the presence of KCl, the distribution of sterols and PI(4,5)P2 was different from that of the WT, which might contribute to altered membrane polarization and ion homeostasis in the mutant. ------- COMMENT: 6464e04ca419e15b 80 l9bUbItbV1zP5DORdCoolq3vLBE (Figure 8) We found that apm1delta, gga22delta, and gga21delta gga22delta (denoted by ggadeltadelta in the Figure 8) were sensitive to both high concentrations of K+ salts and sorbitol (Figure 8), which showed that they were defective in growth under osmotic stress. ------- COMMENT: 6464e04ca419e15b 81 l9bUbItbV1zP5DORdCoolq3vLBE (Figure 8) We found that apm1delta, gga22delta, and gga21delta gga22delta (denoted by ggadeltadelta in the Figure 8) were sensitive to both high concentrations of K+ salts and sorbitol (Figure 8), which showed that they were defective in growth under osmotic stress. ------- COMMENT: 6464e04ca419e15b 82 l9bUbItbV1zP5DORdCoolq3vLBE (Figure 8) We found that apm1delta, gga22delta, and gga21delta gga22delta (denoted by ggadeltadelta in the Figure 8) were sensitive to both high concentrations of K+ salts and sorbitol (Figure 8), which showed that they were defective in growth under osmotic stress. ------- COMMENT: 6464e04ca419e15b 83 l9bUbItbV1zP5DORdCoolq3vLBE (Figure 8) We found that apm1delta, gga22delta, and gga21delta gga22delta (denoted by ggadeltadelta in the Figure 8) were sensitive to both high concentrations of K+ salts and sorbitol (Figure 8), which showed that they were defective in growth under osmotic stress. ------- COMMENT: 6464e04ca419e15b 84 l9bUbItbV1zP5DORdCoolq3vLBE (Figure 8) We found that apm1delta, gga22delta, and gga21delta gga22delta (denoted by ggadeltadelta in the Figure 8) were sensitive to both high concentrations of K+ salts and sorbitol (Figure 8), which showed that they were defective in growth under osmotic stress. ------- COMMENT: 6464e04ca419e15b 85 vrVvcV2fXywI5ka4H9SzyttGk5Y We found that apm1delta, gga22delta, and gga21delta gga22delta (denoted by gga11 in the Figure 8) were sensitive to both high concentrations of K+ salts and sorbitol (Figure 8), which showed that they were defective in growth under osmotic stress. ------- COMMENT: 6464e04ca419e15b 86 VB7I/2UxaVPgoeRqKiAAQSck3K4 (Figure 8) All mutants exhibited a degree of sensitivity to hygromycin B that was alleviated by KCl, an indication of membrane hyperpolarization. ------- COMMENT: 6464e04ca419e15b 87 DR7x1HO71pfr7hvSDUwqsCRwncg All mutants exhibited a degree of sensitivity to hygromycin B that was alleviated by KCl, an indication of membrane hyperpolarization. ------- COMMENT: 6464e04ca419e15b 88 VB7I/2UxaVPgoeRqKiAAQSck3K4 (Figure 8) All mutants exhibited a degree of sensitivity to hygromycin B that was alleviated by KCl, an indication of membrane hyperpolarization. ------- COMMENT: 6464e04ca419e15b 89 VB7I/2UxaVPgoeRqKiAAQSck3K4 (Figure 8) All mutants exhibited a degree of sensitivity to hygromycin B that was alleviated by KCl, an indication of membrane hyperpolarization. ------- COMMENT: 6464e04ca419e15b 90 VB7I/2UxaVPgoeRqKiAAQSck3K4 (Figure 8) All mutants exhibited a degree of sensitivity to hygromycin B that was alleviated by KCl, an indication of membrane hyperpolarization. ------- COMMENT: 6464e04ca419e15b 91 mhGUbxlcwJtzxy152WUeeqcnfZk (Figure 8) gga22delta, which was the only mutant that grew on CaCl2 plates as efficiently as the WT. ------- COMMENT: 6464e04ca419e15b 92 UvE5uPnrwP19DHQaZ723bgbgH9s ent3delta and apm3delta were slightly sensitive to high concentrations of KCl and KNO3 and were very sensitive to CaCl2. (Figure 8) ------- COMMENT: 6464e04ca419e15b 93 UvE5uPnrwP19DHQaZ723bgbgH9s ent3delta and apm3delta were slightly sensitive to high concentrations of KCl and KNO3 and were very sensitive to CaCl2. (Figure 8) ------- COMMENT: 6486be0c077d9f0b 2 ZePZl1HQwXmfpBx5DwAjJw8bzog (comment: assay construct also has nt change G36C to distinguish from snu2+ transcript) ------- COMMENT: 6486be0c077d9f0b 3 ZePZl1HQwXmfpBx5DwAjJw8bzog (comment: assay construct also has nt change G36C to distinguish from snu2+ transcript) ------- COMMENT: 6486be0c077d9f0b 4 ZePZl1HQwXmfpBx5DwAjJw8bzog) ------- COMMENT: 6486be0c077d9f0b 5 ZePZl1HQwXmfpBx5DwAjJw8bzog (comment: assay construct also has nt change G36C to distinguish from snu2+ transcript) ------- COMMENT: 6486be0c077d9f0b 7 ZePZl1HQwXmfpBx5DwAjJw8bzog (comment: assay construct also has nt change G36C to distinguish from snu2+ transcript) ------- COMMENT: 650057099746f1a6 7 XSkqB/YYSMhdKaAI8MnlvwrNl3g (comment: CHECK increased centromere spindle pole body detachment during meiotic prophase fission-yeast-phenotype/2055/) ------- COMMENT: 652d207adb4776b2 1 88B/FF9PvhJN9wjH6WXmnFBBq5Q figure 2a ------- COMMENT: 652d207adb4776b2 3 dM+N58zMLC+TFF8/Buk6lzLvN68 Figure 2D & 4B ------- COMMENT: 652d207adb4776b2 6 od+EBxKErbrxOh+KczJ9KOGsCUk Figure 3B ------- COMMENT: 652d207adb4776b2 7 W4egHp8nZtd5iNYpwU05IAEyjwY Figure 3d ------- COMMENT: 652d207adb4776b2 8 t6uyTtt4P/8kRlIjC2qelSNbzfs Fig 3C ------- COMMENT: 652d207adb4776b2 9 5zLBcPfE96G7rFQPJBZsaEOa1Og Fig 4C ------- COMMENT: 652d207adb4776b2 10 b+00gXuAJVmG6MoHxSub6s9WxVw Figure 4C ------- COMMENT: 652d207adb4776b2 20 T7TKFiCgC/Wkl8l0s9QdUf6uz8o figure S2B ------- COMMENT: 652d207adb4776b2 23 EkXghvkrJyLgiI/AlLB/pPk2wQM figure S2 ------- COMMENT: 652d207adb4776b2 24 1B6HhiwJMAOCifEWPO0swRJikmQ Figure 3F ------- COMMENT: 652d207adb4776b2 25 5bxdQz7qO+FKJb8z6lMp4Sqd4iI Figure 3F (comment: DECOUPLED CELL GROWTH AND DIVISION) ------- COMMENT: 652d207adb4776b2 26 1Q75bToClluyPKBy2asEwqlWAtQ figure 5a ------- COMMENT: 652d207adb4776b2 29 9hFhw4nPm7a/9h5+AZNYtEK4T1w However, no difference in CaMKKSsp1 protein levels or phos- ppk34 phorylation status was observed in CaMKK .D mutants compared with wild-type cells (Figures S4A and S4B ------- COMMENT: 652d207adb4776b2 30 ywMJkiJ/7+18+NQSCeWd0IIj5ZU Fig 3G ------- COMMENT: 652d207adb4776b2 33 OUlqB0hqYDc3g+kvIEn8yGOQD8g Figure S3A ------- COMMENT: 652d207adb4776b2 38 foLZvNwPomujfsi3oAbbxeywskY fig 6A ------- COMMENT: 652d207adb4776b2 39 x9BTPxoewRoMmL6Tsv8ZTO/Oftc figure 6E ------- COMMENT: 652d207adb4776b2 41 x9BTPxoewRoMmL6Tsv8ZTO/Oftc figure 6E ------- COMMENT: 652d207adb4776b2 46 88B/FF9PvhJN9wjH6WXmnFBBq5Q figure 2a ------- COMMENT: 652d207adb4776b2 47 DfRbQA+kL2adthxMDtu8brF+z6k figure 2b (comment: 9% longer) ------- COMMENT: 652d207adb4776b2 50 H6o1YTu0tHopE6EbDvwyEzCMFu4 figure 4D ------- COMMENT: 652d207adb4776b2 51 98yOAkKlFq0VEDkssdZRekKzZ0A figure 4E ------- COMMENT: 652d207adb4776b2 52 H6o1YTu0tHopE6EbDvwyEzCMFu4 figure 4D ------- COMMENT: 652d207adb4776b2 53 Gs119QRjOJV2Fm4ooJFV5fX+kQ4 An increase in AMPKaSsp2 Thr189 phosphorylation was also observed in the cbs2 .D AMPKg .D double mutant (Figure S2F). ------- COMMENT: 652d207adb4776b2 54 b45sTRGH+h3Lg+C+D2kKGvQmDfg (comment: CHECK causally upstream of ssp2) ------- COMMENT: 652d207adb4776b2 55 9hFhw4nPm7a/9h5+AZNYtEK4T1w However, no difference in CaMKKSsp1 protein levels or phos- ppk34 phorylation status was observed in CaMKK .D mutants compared with wild-type cells (Figures S4A and S4B ------- COMMENT: 652d207adb4776b2 56 jyI2Y+WtnbXsRII/pnwEuhXjzmg fig S4C,D ------- COMMENT: 652d207adb4776b2 57 BE2/2Rt5qyfJbsrlEXLCFliQ7k8 Fig 6G ------- COMMENT: 652d207adb4776b2 58 b45sTRGH+h3Lg+C+D2kKGvQmDfg (comment: CHECK causally upstream of ssp2) ------- COMMENT: 65ecf5af1bf30a8a 2 bMmRRMYm9z9EBkkvE6FH9tjrvAc From these results, we conclude that the F330A mutation significantly reduces the affinity of the Mud1 UBA domain for K48-linked polyUb chains without significantly affecting monoUb binding. ------- COMMENT: 65ecf5af1bf30a8a 3 bMmRRMYm9z9EBkkvE6FH9tjrvAc From these results, we conclude that the F330A mutation significantly reduces the affinity of the Mud1 UBA domain for K48-linked polyUb chains without significantly affecting monoUb binding. ------- COMMENT: 65edebcc485f98a8 2 L0PYFrYfmS/5urxXZRT98DOxJ8g ------- COMMENT: 6601e49e3b15b194 34 aGIo9k20nCsYI4d8IM9DW41PbrA localization depends on microtubule cytoskeleton, as determined by treatment with carbendazim (methyl 2-benzimidazolecarbamate; MBC), and on actin cytoskeleton, as determined by treatment with latrunculin B or cytochalasin D ------- COMMENT: 660a55ae599b5919 1 8RG0hmC4BGFWqoqXJe3kGNb5r34 (comment: inability to take up 14-C uracil in fur4 deletion mutant) ------- COMMENT: 660a55ae599b5919 4 O6SqFiBjgnbQXmXQ0fjmiAiPjhg (comment: CONDITION nitrogen rich) ------- COMMENT: 660a55ae599b5919 5 75t8fw5V7jFAcahQ+4PnfqAGZZY (comment: CONDITION grown in EMM or YES medium) ------- COMMENT: 660a55ae599b5919 9 yWDbAl+xWu9i5kIQiyDa8usA1Jo (comment: CHECK uracil auxotroph) ------- COMMENT: 660a55ae599b5919 10 ynLIJTlleC2qqOxdbrLRBp5RAms (comment: CHECK auxotrophic for cytosine, uridine and UMP) ------- COMMENT: 660a55ae599b5919 11 Ph42epaXJmY+whUc/PvtuSh+E3s (comment: CHECK cell lysis on uracil depleted medium) ------- COMMENT: 660a55ae599b5919 12 Wjs/wbfRZxYKnkwe+3WsyO3lxRQ (comment: uracil uptake enhancement in pub1 deletion) ------- COMMENT: 660a55ae599b5919 13 5Q/pWprV2TEda7z2QCxUKvMH8oA (comment: CHECK cell lysis in YPD) ------- COMMENT: 660a55ae599b5919 17 O6SqFiBjgnbQXmXQ0fjmiAiPjhg (comment: CONDITION nitrogen rich) ------- COMMENT: 660a55ae599b5919 18 RYvdMNm+9FnVikueNfE51SEULW0 (comment: CHECK assayed_using(PomBase:fur4)) ------- COMMENT: 661292025a8cdee8 3 LERyNth0h5ka1mzVKx+RIK7pMLU Glucosidase 1, a type II membrane protein with a luminal hydrolytic domain, removes the outermost glucose from protein-linked Glc3Man9GlcNAc2 in the endoplasmic reticulum ------- COMMENT: 661292025a8cdee8 6 RuESSDMngjuYaFmzGGqeY7mLvzI A suppression of the gls1 mutant phenotype is caused by a simultaneous mutation in the alg10+ gene ------- COMMENT: 661292025a8cdee8 9 yyMNf1x4ofpAA8TPiE7bhUbV4SU fig 3b ------- COMMENT: 661292025a8cdee8 10 4yCVBSkl6bg2aTrDdCZ1ZS1USlw fig 5b ------- COMMENT: 661292025a8cdee8 11 4yCVBSkl6bg2aTrDdCZ1ZS1USlw fig 5b ------- COMMENT: 661292025a8cdee8 13 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: 661292025a8cdee8 15 aR3y1/htBisgf/GTM5jw8a8XvgA fig S4 ------- COMMENT: 661292025a8cdee8 16 aR3y1/htBisgf/GTM5jw8a8XvgA fig S4 ------- COMMENT: 661292025a8cdee8 17 HOYWL8+TXNSBG/Y0x8f2J1StgOg fig 10 ------- COMMENT: 661292025a8cdee8 18 HOYWL8+TXNSBG/Y0x8f2J1StgOg fig 10 ------- COMMENT: 661292025a8cdee8 19 HOYWL8+TXNSBG/Y0x8f2J1StgOg fig 10 ------- COMMENT: 661292025a8cdee8 21 UYJ2NtICld/3sR9AYnq8oypEy+s figure 5b and S2 ------- COMMENT: 661292025a8cdee8 22 BMFRkLsNO9mUubr6poQgSyvm86U figure 5c ------- COMMENT: 661292025a8cdee8 23 BMFRkLsNO9mUubr6poQgSyvm86U figure 5c ------- COMMENT: 661292025a8cdee8 24 oXTu2cxtZ9ZZW2UaxbaIntoVp3E figure 5c (comment: CHECK hypoglycosylation) ------- COMMENT: 661292025a8cdee8 25 Ux3EKIqjY5c7DF66teUnH2Zo5Rk figure 5 and 11 (comment: no loss of viability ) ------- COMMENT: 661292025a8cdee8 27 FJrUrY/cNbL7O7xtjP6w5yuBdIM fig 2b ------- COMMENT: 661292025a8cdee8 28 FJrUrY/cNbL7O7xtjP6w5yuBdIM fig 2b ------- COMMENT: 661292025a8cdee8 29 4yCVBSkl6bg2aTrDdCZ1ZS1USlw fig 5b ------- COMMENT: 661292025a8cdee8 30 4yCVBSkl6bg2aTrDdCZ1ZS1USlw fig 5b ------- COMMENT: 661292025a8cdee8 31 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: 6631b1666d57256f 1 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: 6631b1666d57256f 2 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: 6631b1666d57256f 3 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 6631b1666d57256f 5 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 6631b1666d57256f 6 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 6631b1666d57256f 7 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 6631b1666d57256f 8 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: 6631b1666d57256f 18 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 6631b1666d57256f 19 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 664a66149791e18c 7 /74PJyvK3Fj087REbNKg7K63rXM during mitotic G2 arrest ------- COMMENT: 6673f828e280c8b9 1 lcRgvO8GdGFjyZsZVxPB1rxdz9A (Figure 3D) ------- COMMENT: 6673f828e280c8b9 2 lcRgvO8GdGFjyZsZVxPB1rxdz9A (Figure 3D) ------- COMMENT: 6673f828e280c8b9 4 lcRgvO8GdGFjyZsZVxPB1rxdz9A (Figure 3D) ------- COMMENT: 6673f828e280c8b9 5 lcRgvO8GdGFjyZsZVxPB1rxdz9A (Figure 3D) ------- COMMENT: 6673f828e280c8b9 7 nu5a7mCiNzxXHCBgrB8EzcYauIU Scs2N bound specifically to PI and PS, and Scs2N2TA showed weaker binding to both, whereas Scs2N3KA exhibited significantly lower affinities for both (Figures 3C, S3D, and S3E). (Liposome binding assay) ------- COMMENT: 6673f828e280c8b9 8 GSuGiPVNVLtAJkNnHSVUAU6G94Q Scs2N bound specifically to PI and PS, and Scs2N2TA showed weaker binding to both, whereas Scs2N3KA exhibited significantly lower affinities for both (Figures 3C, S3D, and S3E). (liposome binding assay) ------- COMMENT: 6673f828e280c8b9 9 qEWsZA3p2PXT5JZvw3GSlafPsu8 We found that both the PM and Golgi pools of PI4P decreased significantly in sec14D cells. ------- COMMENT: 6673f828e280c8b9 10 yuAi40RlduR6snkWQZv78Lxheak Such reduction was enhanced when cells further lost either or both of csr102 and pdr16 in the background, whereas mutants with single or double deletion of csr102 and pdr16 remained WT-like (Figure 2D). ------- COMMENT: 6673f828e280c8b9 11 yuAi40RlduR6snkWQZv78Lxheak Such reduction was enhanced when cells further lost either or both of csr102 and pdr16 in the background, whereas mutants with single or double deletion of csr102 and pdr16 remained WT-like (Figure 2D). ------- COMMENT: 6673f828e280c8b9 12 yuAi40RlduR6snkWQZv78Lxheak Such reduction was enhanced when cells further lost either or both of csr102 and pdr16 in the background, whereas mutants with single or double deletion of csr102 and pdr16 remained WT-like (Figure 2D). ------- COMMENT: 6673f828e280c8b9 20 GNRYEV4gVakXhyUkpWpADdJP9CE Such interactions were required for its cortical localization, as Csr102 became cytosolic in the absence of both VAPs even when ER-PM contacts were artificially restored (Figures 2E and S2F). ------- COMMENT: 6673f828e280c8b9 21 D35R078npiFfTyiMIXElkDczm5o medium pH below 3.5. In fact, scs2Dscs22D cells already showed compromised growth in highly acidic media, similarly to pma1-DCter cells that lacked the C-terminal R domain (Figure 6D). ------- COMMENT: 6673f828e280c8b9 22 SObobNRDxXz0Pvn3JTTVheGO5dk (comment: CONDITION medium pH below 3.5) ------- COMMENT: 6673f828e280c8b9 23 ipbscizMcz/4pTWc7I1zDsnf7mc (comment: CONDITION medium pH below 3.5) (Figure 6) ------- COMMENT: 6673f828e280c8b9 24 h3AjEtNT5xYZbe1l9VBqlaLd14U (comment: CONDITION medium pH below 3.5) (Figure 6) ------- COMMENT: 6673f828e280c8b9 25 h3AjEtNT5xYZbe1l9VBqlaLd14U (comment: CONDITION medium pH below 3.5) (Figure 6) ------- COMMENT: 6673f828e280c8b9 26 h3AjEtNT5xYZbe1l9VBqlaLd14U (comment: CONDITION medium pH below 3.5) (Figure 6) ------- COMMENT: 6673f828e280c8b9 27 hMfuStwvFumCEZqrecJZuOpAz0w Either the removal or genetic alterations of this presumed Scs2-interacting motif engendered inviable spores in S. pombe (Figure 6A), indicative of its necessity for Pma1 function. ------- COMMENT: 6673f828e280c8b9 28 hMfuStwvFumCEZqrecJZuOpAz0w Either the removal or genetic alterations of this presumed Scs2-interacting motif engendered inviable spores in S. pombe (Figure 6A), indicative of its necessity for Pma1 function. ------- COMMENT: 6673f828e280c8b9 29 hMfuStwvFumCEZqrecJZuOpAz0w Either the removal or genetic alterations of this presumed Scs2-interacting motif engendered inviable spores in S. pombe (Figure 6A), indicative of its necessity for Pma1 function. ------- COMMENT: 6673f828e280c8b9 30 XoMSPkGCQoUNJ8IJCpr27MA76mg (Figure S2A) ------- COMMENT: 6673f828e280c8b9 31 XoMSPkGCQoUNJ8IJCpr27MA76mg (Figure S2A) ------- COMMENT: 6673f828e280c8b9 32 epJnUpnqh8uU2VsMe8S4cZAo7/I Figure 2B, Figure S2A ------- COMMENT: 6673f828e280c8b9 33 XoMSPkGCQoUNJ8IJCpr27MA76mg (Figure S2A) ------- COMMENT: 6673f828e280c8b9 34 XoMSPkGCQoUNJ8IJCpr27MA76mg (Figure S2A) ------- COMMENT: 6673f828e280c8b9 35 epJnUpnqh8uU2VsMe8S4cZAo7/I Figure 2B, Figure S2A ------- COMMENT: 6673f828e280c8b9 36 epJnUpnqh8uU2VsMe8S4cZAo7/I Figure 2B, Figure S2A ------- COMMENT: 6673f828e280c8b9 37 epJnUpnqh8uU2VsMe8S4cZAo7/I Figure 2B, Figure S2A ------- COMMENT: 6673f828e280c8b9 38 XoMSPkGCQoUNJ8IJCpr27MA76mg (Figure S2A) ------- COMMENT: 6673f828e280c8b9 39 XoMSPkGCQoUNJ8IJCpr27MA76mg (Figure S2A) ------- COMMENT: 6673f828e280c8b9 40 XoMSPkGCQoUNJ8IJCpr27MA76mg (Figure S2A) ------- COMMENT: 6673f828e280c8b9 41 XoMSPkGCQoUNJ8IJCpr27MA76mg (Figure S2A) ------- COMMENT: 6673f828e280c8b9 42 XoMSPkGCQoUNJ8IJCpr27MA76mg (Figure S2A) ------- COMMENT: 6673f828e280c8b9 43 XoMSPkGCQoUNJ8IJCpr27MA76mg (Figure S2A) ------- COMMENT: 6673f828e280c8b9 44 XoMSPkGCQoUNJ8IJCpr27MA76mg (Figure S2A) ------- COMMENT: 6673f828e280c8b9 46 XoMSPkGCQoUNJ8IJCpr27MA76mg (Figure S2A) ------- COMMENT: 6673f828e280c8b9 48 XoMSPkGCQoUNJ8IJCpr27MA76mg (Figure S2A) ------- COMMENT: 6673f828e280c8b9 50 XoMSPkGCQoUNJ8IJCpr27MA76mg (Figure S2A) ------- COMMENT: 6673f828e280c8b9 52 XoMSPkGCQoUNJ8IJCpr27MA76mg (Figure S2A) ------- COMMENT: 6673f828e280c8b9 54 XoMSPkGCQoUNJ8IJCpr27MA76mg (Figure S2A) ------- COMMENT: 6673f828e280c8b9 56 XoMSPkGCQoUNJ8IJCpr27MA76mg (Figure S2A) ------- COMMENT: 6673f828e280c8b9 58 +Yy3ZgHOMaG5QfAVykI3YiNJREI (comment: liposome binding assay) ------- COMMENT: 6673f828e280c8b9 59 4p2Pysim/qcF2Z5TD0Zh+wm/jXw (comment: FFAT-like motif that binds VAPs (scs2)) ------- COMMENT: 6673f828e280c8b9 60 0l5xy/tgEgaj44BfmGnhb/rXKv0 A previous study has shown that Scs2 plays a dominant role in ER-PM tethering over Scs22 in fission yeast,5 manifested by apparent ER-PM dissocia- tion indicated by the luminal ER marker mCherry-ADEL18 in scs2D cells (see also Figure 1A). ------- COMMENT: 6673f828e280c8b9 61 L7kJynRZn1cJgndvW+Zqfk6myws Inter- estingly, ER-PM association was compromised when the open reading frames of scs2 and scs22 were swapped at their genomic loci (asterisked in Figure S1A), implying that two VAPs may intrin- sically differ in ER-PM tethering function. ------- COMMENT: 6673f828e280c8b9 62 mJdqTbihKl2e+YsfnjzmejDEipI (Figure 1C) ------- COMMENT: 6673f828e280c8b9 63 mJdqTbihKl2e+YsfnjzmejDEipI (Figure 1C) ------- COMMENT: 6673f828e280c8b9 64 9C1pC0in+wIFxx8o4Px7qwt5+zE Unlike Scs22 where its nuclear envelope (NE) localization was obvious, the NE pool of Scs2 was barely visible, suggesting likely stronger affinity of Scs2 toward the cell cortex (Figure 1C). ------- COMMENT: 6673f828e280c8b9 65 fscyhHYhz7TqY+0EqsnQsIYK3XQ Notably, mutations of three previously reported conserved lysine residues (K36/38/43A,20,21 hereafter referred to as 3KA) in the VAP consensus sequence (VCS) within the MSP domain of either Scs2 or Scs22 abolished their ER-PM tethering capacity (asterisked by large PM regions devoid of the cER in Figure 1C) ------- COMMENT: 6673f828e280c8b9 66 fscyhHYhz7TqY+0EqsnQsIYK3XQ Notably, mutations of three previously reported conserved lysine residues (K36/38/43A,20,21 hereafter referred to as 3KA) in the VAP consensus sequence (VCS) within the MSP domain of either Scs2 or Scs22 abolished their ER-PM tethering capacity (asterisked by large PM regions devoid of the cER in Figure 1C) ------- COMMENT: 6673f828e280c8b9 67 fs9bhHjJwmH1oHlOtqOtBJOxZ4Q Such reduction was enhanced when cells further lost either or both of csr102 and pdr16 in the background, whereas mutants with single or double deletion of csr102 and pdr16 remained WT-like (Figure 2D). No detectable change of phosphatidylinositol 4,5-bisphosphate (PI4,5P2) levels was seen in all these mutants (data not shown). These data sug- gest that Sec14 family proteins contribute to PI4P homeostasis in S. pombe. ------- COMMENT: 6673f828e280c8b9 68 fs9bhHjJwmH1oHlOtqOtBJOxZ4Q Such reduction was enhanced when cells further lost either or both of csr102 and pdr16 in the background, whereas mutants with single or double deletion of csr102 and pdr16 remained WT-like (Figure 2D). No detectable change of phosphatidylinositol 4,5-bisphosphate (PI4,5P2) levels was seen in all these mutants (data not shown). These data sug- gest that Sec14 family proteins contribute to PI4P homeostasis in S. pombe. ------- COMMENT: 6673f828e280c8b9 69 qEWsZA3p2PXT5JZvw3GSlafPsu8 We found that both the PM and Golgi pools of PI4P decreased significantly in sec14D cells. ------- COMMENT: 6673f828e280c8b9 70 fs9bhHjJwmH1oHlOtqOtBJOxZ4Q Such reduction was enhanced when cells further lost either or both of csr102 and pdr16 in the background, whereas mutants with single or double deletion of csr102 and pdr16 remained WT-like (Figure 2D). No detectable change of phosphatidylinositol 4,5-bisphosphate (PI4,5P2) levels was seen in all these mutants (data not shown). These data sug- gest that Sec14 family proteins contribute to PI4P homeostasis in S. pombe. ------- COMMENT: 6673f828e280c8b9 71 BvKK294YjHQbozYAAIUeSeEGaxk However, no reduction of ER-PM contacts was seen in csr102Dpdr16Dpil1D cells, and Scs2 could still establish ER-PM contacts at cortical regions devoid of Pma1 in the background (Figure 2G) ------- COMMENT: 6673f828e280c8b9 72 r37rcOYYHR42DkvwXadLMLaxy2k Figure S3A (left panel), ER-PM tethering is reduced in Scs2-2TA mutant at native locus ------- COMMENT: 6673f828e280c8b9 73 sL8xJfBp03yBfnXba61aoWYvTIk Fasci- natingly, though not as fully efficient as WT, Scs2-PM was able to establish ER-PM contacts in a dose-dependent manner, which similarly required a functional MSP domain (Figure 3A). ------- COMMENT: 6673f828e280c8b9 74 HT837c3LTWbjato27tCOD4qXJUk Surpris- ingly, like WT (Figure S1F), Scs2-PM was able to restore the PM PI4P level in scs2Dscs22D cells, while Scs23KA-PM again lost the competence (Figure 3B). ------- COMMENT: 6673f828e280c8b9 75 niK3lYyauvsrEGa+gbb/JlsKdoY Likewise, the cortical recruitment of Csr102 was fully rescued by Scs2-PM but not Scs23KA-PM (Figure S3B). ------- COMMENT: 6673f828e280c8b9 76 Dnz7+UOCnY65rw2vIzCOW7BwJEo In fact, purified Scs2N-6His could bind both PI and PS liposomes. Such binding was enhanced with increased PI or PS concentration or by adding PI4P to respective liposomes (Figure S3C). ------- COMMENT: 6673f828e280c8b9 77 Dnz7+UOCnY65rw2vIzCOW7BwJEo In fact, purified Scs2N-6His could bind both PI and PS liposomes. Such binding was enhanced with increased PI or PS concentration or by adding PI4P to respective liposomes (Figure S3C). ------- COMMENT: 6673f828e280c8b9 78 mZZTVsbqmLXkihjzA0+9N3motL8 Indeed, as previously shown,31 the PM pool of PS marked by GFP-Lact-C234 was absent in pps1D (Figure S4A). ------- COMMENT: 6673f828e280c8b9 79 8pCEBflTM0J5Q14JCh6yYWk1DSI We also saw augmented PM levels of PI4P and PI4,5P2 indicated by PHOsh2-GFP and PHNum1- GFP,5 respectively, in these cells (Fig- ure S4A), ------- COMMENT: 6673f828e280c8b9 80 SlCenvY0lvgChSuIyGl4MwP4kUU ER-PM contact formation was however un- affected in pps1D (Figures 4B and S4B). ------- COMMENT: 6673f828e280c8b9 81 hwrHUoaap9H276OmLx582q/iqkw Figures 4B and S4B) ------- COMMENT: 6673f828e280c8b9 84 8zdno63XFs3BFsVP161LRtfCMNM Figure 3D ------- COMMENT: 6673f828e280c8b9 85 8zdno63XFs3BFsVP161LRtfCMNM Figure 3D ------- COMMENT: 6673f828e280c8b9 86 r37rcOYYHR42DkvwXadLMLaxy2k Figure S3A (left panel), ER-PM tethering is reduced in Scs2-2TA mutant at native locus ------- COMMENT: 6673f828e280c8b9 87 fscyhHYhz7TqY+0EqsnQsIYK3XQ Notably, mutations of three previously reported conserved lysine residues (K36/38/43A,20,21 hereafter referred to as 3KA) in the VAP consensus sequence (VCS) within the MSP domain of either Scs2 or Scs22 abolished their ER-PM tethering capacity (asterisked by large PM regions devoid of the cER in Figure 1C) ------- COMMENT: 669a6e624422bdf5 1 vqEl6bAsFVuBkhebmKe/naLqx0w Af®nity-puri®ed Clr6-HA and control wild-type fractions were incubated with [3H]acetyl- labelled histones. Quantitation of released [3H]acetyl groups revealed that the Clr6-HA fraction possesses deacetylase activity and that this activity is sensitive to trichostatin A (TSA), a speci®c inhibitor of HDACs (Figure 1C). ------- COMMENT: 669a6e624422bdf5 2 Uktko17wh6QzNeOB2D06ZJb2C0A Identi®cation of Clr6-associated proteins, (Figure 1B)/ Alp13, Prw1 and Pst2 are stably associated with Clr6 in vivo (Figure 3A) ------- COMMENT: 669a6e624422bdf5 3 bKaeQ8wbB1XFOMHYc4JLJWf5xoQ Identi®cation of Clr6-associated proteins, (Figure 1B)/Alp13, Prw1 and Pst2 are stably associated with Clr6 in vivo (Figure 3A) ------- COMMENT: 669a6e624422bdf5 4 bKaeQ8wbB1XFOMHYc4JLJWf5xoQ Identi®cation of Clr6-associated proteins, (Figure 1B)/Alp13, Prw1 and Pst2 are stably associated with Clr6 in vivo (Figure 3A) ------- COMMENT: 669a6e624422bdf5 5 cfL8za15FyFlIqV/dBcU7QrzWUg Identi®cation of Clr6-associated proteins, (Figure 1B) /Alp13, Prw1 and Pst2 are stably associated with Clr6 in vivo (Figure 3A) ------- COMMENT: 669a6e624422bdf5 6 bk1eB0qbDQXEAoBacm14CB80RoI Our analyses showed that all four proteins were localized predominantly in the nucleus on chromatin but excluded from the nucleolus (Figure 4A and B). ------- COMMENT: 669a6e624422bdf5 7 bk1eB0qbDQXEAoBacm14CB80RoI Our analyses showed that all four proteins were localized predominantly in the nucleus on chromatin but excluded from the nucleolus (Figure 4A and B). ------- COMMENT: 669a6e624422bdf5 8 bk1eB0qbDQXEAoBacm14CB80RoI Our analyses showed that all four proteins were localized predominantly in the nucleus on chromatin but excluded from the nucleolus (Figure 4A and B). ------- COMMENT: 669a6e624422bdf5 9 bk1eB0qbDQXEAoBacm14CB80RoI Our analyses showed that all four proteins were localized predominantly in the nucleus on chromatin but excluded from the nucleolus (Figure 4A and B). ------- COMMENT: 669a6e624422bdf5 10 bk1eB0qbDQXEAoBacm14CB80RoI Our analyses showed that all four proteins were localized predominantly in the nucleus on chromatin but excluded from the nucleolus (Figure 4A and B). ------- COMMENT: 669a6e624422bdf5 11 bk1eB0qbDQXEAoBacm14CB80RoI Our analyses showed that all four proteins were localized predominantly in the nucleus on chromatin but excluded from the nucleolus (Figure 4A and B). ------- COMMENT: 669a6e624422bdf5 12 bk1eB0qbDQXEAoBacm14CB80RoI Our analyses showed that all four proteins were localized predominantly in the nucleus on chromatin but excluded from the nucleolus (Figure 4A and B). ------- COMMENT: 669a6e624422bdf5 13 bk1eB0qbDQXEAoBacm14CB80RoI Our analyses showed that all four proteins were localized predominantly in the nucleus on chromatin but excluded from the nucleolus (Figure 4A and B). ------- COMMENT: 669a6e624422bdf5 17 /LYEFHQKn58Y9P1SJzR5fpJAwWY sensitivity to DNA-damaging agents [such as UV, methyl methane- sulfonate (MMS) and bleomycin] (Figure 5B±D) ------- COMMENT: 669a6e624422bdf5 18 /LYEFHQKn58Y9P1SJzR5fpJAwWY sensitivity to DNA-damaging agents [such as UV, methyl methane- sulfonate (MMS) and bleomycin] (Figure 5B±D) ------- COMMENT: 669a6e624422bdf5 19 xK1kF8UinyaXY9ZVbH/j+klW1Dc and irreversible tem- perature-sensitive (Ts±) growth defects (Figure 5B±D). ------- COMMENT: 669a6e624422bdf5 20 /LYEFHQKn58Y9P1SJzR5fpJAwWY sensitivity to DNA-damaging agents [such as UV, methyl methane- sulfonate (MMS) and bleomycin] (Figure 5B±D) ------- COMMENT: 669a6e624422bdf5 21 /LYEFHQKn58Y9P1SJzR5fpJAwWY sensitivity to DNA-damaging agents [such as UV, methyl methane- sulfonate (MMS) and bleomycin] (Figure 5B±D) ------- COMMENT: 669a6e624422bdf5 22 /LYEFHQKn58Y9P1SJzR5fpJAwWY sensitivity to DNA-damaging agents [such as UV, methyl methane- sulfonate (MMS) and bleomycin] (Figure 5B±D) ------- COMMENT: 669a6e624422bdf5 23 /LYEFHQKn58Y9P1SJzR5fpJAwWY sensitivity to DNA-damaging agents [such as UV, methyl methane- sulfonate (MMS) and bleomycin] (Figure 5B±D) ------- COMMENT: 669a6e624422bdf5 24 xK1kF8UinyaXY9ZVbH/j+klW1Dc and irreversible tem- perature-sensitive (Ts±) growth defects (Figure 5B±D). ------- COMMENT: 669a6e624422bdf5 25 xK1kF8UinyaXY9ZVbH/j+klW1Dc and irreversible tem- perature-sensitive (Ts±) growth defects (Figure 5B±D). ------- COMMENT: 669a6e624422bdf5 26 xK1kF8UinyaXY9ZVbH/j+klW1Dc and irreversible tem- perature-sensitive (Ts±) growth defects (Figure 5B±D). ------- COMMENT: 669a6e624422bdf5 27 /LYEFHQKn58Y9P1SJzR5fpJAwWY sensitivity to DNA-damaging agents [such as UV, methyl methane- sulfonate (MMS) and bleomycin] (Figure 5B±D) ------- COMMENT: 669a6e624422bdf5 28 /LYEFHQKn58Y9P1SJzR5fpJAwWY sensitivity to DNA-damaging agents [such as UV, methyl methane- sulfonate (MMS) and bleomycin] (Figure 5B±D) ------- COMMENT: 669a6e624422bdf5 29 /LYEFHQKn58Y9P1SJzR5fpJAwWY sensitivity to DNA-damaging agents [such as UV, methyl methane- sulfonate (MMS) and bleomycin] (Figure 5B±D) ------- COMMENT: 669a6e624422bdf5 30 /LYEFHQKn58Y9P1SJzR5fpJAwWY sensitivity to DNA-damaging agents [such as UV, methyl methane- sulfonate (MMS) and bleomycin] (Figure 5B±D) ------- COMMENT: 669a6e624422bdf5 31 /LYEFHQKn58Y9P1SJzR5fpJAwWY sensitivity to DNA-damaging agents [such as UV, methyl methane- sulfonate (MMS) and bleomycin] (Figure 5B±D) ------- COMMENT: 669a6e624422bdf5 32 /LYEFHQKn58Y9P1SJzR5fpJAwWY sensitivity to DNA-damaging agents [such as UV, methyl methane- sulfonate (MMS) and bleomycin] (Figure 5B±D) ------- COMMENT: 669a6e624422bdf5 33 WqqGGQRabkfKz/ohtyEi7YfnIN8 Moreover, Dprw1 cells showed an increase in acetylation of H3 Lys9 and Lys14. ------- COMMENT: 669a6e624422bdf5 34 z69RWb95LEzYzgvfHXj2xKFfj1k Cells carrying Dalp13 were speci®cally defective in the removal of acetyl groups present on H3 Lys9 and Lys14, as well as H4 Lys5 and Lys8. ------- COMMENT: 669a6e624422bdf5 35 7RTJvEEw3TPcszDOaazucRCHCug The results presented in Figure 5E demonstrate that mutant strains show a signi®cant increase in histone acetylation levels compared with the wild-type control. Mutation in clr6 results in elevated acetylation levels at all residues tested on the histone H3 and H4 tails. ------- COMMENT: 669a6e624422bdf5 36 qO/fxTba3rwMEpjWXoKUo2hd4E0 The Dpst2 cells showed a signi®cant increase in acetylation of most lysine residues on H3 and H4 tails, except H4 Lys8. ------- COMMENT: 669a6e624422bdf5 37 qO/fxTba3rwMEpjWXoKUo2hd4E0 The Dpst2 cells showed a signi®cant increase in acetylation of most lysine residues on H3 and H4 tails, except H4 Lys8. ------- COMMENT: 669a6e624422bdf5 38 7RTJvEEw3TPcszDOaazucRCHCug The results presented in Figure 5E demonstrate that mutant strains show a signi®cant increase in histone acetylation levels compared with the wild-type control. Mutation in clr6 results in elevated acetylation levels at all residues tested on the histone H3 and H4 tails. ------- COMMENT: 669a6e624422bdf5 39 z69RWb95LEzYzgvfHXj2xKFfj1k Cells carrying Dalp13 were speci®cally defective in the removal of acetyl groups present on H3 Lys9 and Lys14, as well as H4 Lys5 and Lys8. ------- COMMENT: 669a6e624422bdf5 41 Ci9Hl/0oSWzKXg4yycmvESt2fuw Interestingly, we noticed that Dprw1 causes an increase in the acetylation of H4 Lys5 and Lys12, a pattern of acetylation known to be associated with newly synthesized histones (Sobel et al., 1995). ------- COMMENT: 669a6e624422bdf5 42 Ci9Hl/0oSWzKXg4yycmvESt2fuw Interestingly, we noticed that Dprw1 causes an increase in the acetylation of H4 Lys5 and Lys12, a pattern of acetylation known to be associated with newly synthesized histones (Sobel et al., 1995). ------- COMMENT: 669a6e624422bdf5 43 WqqGGQRabkfKz/ohtyEi7YfnIN8 Moreover, Dprw1 cells showed an increase in acetylation of H3 Lys9 and Lys14. ------- COMMENT: 669a6e624422bdf5 44 z69RWb95LEzYzgvfHXj2xKFfj1k Cells carrying Dalp13 were speci®cally defective in the removal of acetyl groups present on H3 Lys9 and Lys14, as well as H4 Lys5 and Lys8. ------- COMMENT: 669a6e624422bdf5 45 z69RWb95LEzYzgvfHXj2xKFfj1k Cells carrying Dalp13 were speci®cally defective in the removal of acetyl groups present on H3 Lys9 and Lys14, as well as H4 Lys5 and Lys8. ------- COMMENT: 669a6e624422bdf5 46 qO/fxTba3rwMEpjWXoKUo2hd4E0 The Dpst2 cells showed a signi®cant increase in acetylation of most lysine residues on H3 and H4 tails, except H4 Lys8. ------- COMMENT: 669a6e624422bdf5 47 qO/fxTba3rwMEpjWXoKUo2hd4E0 The Dpst2 cells showed a signi®cant increase in acetylation of most lysine residues on H3 and H4 tails, except H4 Lys8. ------- COMMENT: 669a6e624422bdf5 48 rrgbHHckIFDev930+jnqAebqOWc These data suggest that Clr6 and its associated factors are involved in the deacetylation of histones in vivo. ------- COMMENT: 669a6e624422bdf5 49 rrgbHHckIFDev930+jnqAebqOWc These data suggest that Clr6 and its associated factors are involved in the deacetylation of histones in vivo. ------- COMMENT: 669a6e624422bdf5 50 rrgbHHckIFDev930+jnqAebqOWc These data suggest that Clr6 and its associated factors are involved in the deacetylation of histones in vivo. ------- COMMENT: 669a6e624422bdf5 51 rrgbHHckIFDev930+jnqAebqOWc These data suggest that Clr6 and its associated factors are involved in the deacetylation of histones in vivo. ------- COMMENT: 669a6e624422bdf5 52 26iIHaJpaiMYmaIY769kbJGYkKw Our analysis revealed that mutant strains display defects in the segregation, resolution and/or condensation of chromosomes during mitosis (Figure 6A), and mis-segre- gated the mini-chromosome Ch16 (a 530 kb derivative of chromosome 3; Niwa et al., 1986) at a signi®cantly higher rate than wild-type cells (Figure 6C). ------- COMMENT: 669a6e624422bdf5 53 26iIHaJpaiMYmaIY769kbJGYkKw Our analysis revealed that mutant strains display defects in the segregation, resolution and/or condensation of chromosomes during mitosis (Figure 6A), and mis-segre- gated the mini-chromosome Ch16 (a 530 kb derivative of chromosome 3; Niwa et al., 1986) at a signi®cantly higher rate than wild-type cells (Figure 6C). ------- COMMENT: 669a6e624422bdf5 54 26iIHaJpaiMYmaIY769kbJGYkKw Our analysis revealed that mutant strains display defects in the segregation, resolution and/or condensation of chromosomes during mitosis (Figure 6A), and mis-segre- gated the mini-chromosome Ch16 (a 530 kb derivative of chromosome 3; Niwa et al., 1986) at a signi®cantly higher rate than wild-type cells (Figure 6C). ------- COMMENT: 669a6e624422bdf5 55 26iIHaJpaiMYmaIY769kbJGYkKw Our analysis revealed that mutant strains display defects in the segregation, resolution and/or condensation of chromosomes during mitosis (Figure 6A), and mis-segre- gated the mini-chromosome Ch16 (a 530 kb derivative of chromosome 3; Niwa et al., 1986) at a signi®cantly higher rate than wild-type cells (Figure 6C). ------- COMMENT: 669a6e624422bdf5 56 taJ02sTIhiiNEe9IoO4RsILBwGY We found that H3 Ser10 phosphorylation levels were considerably reduced in alp13, pst2 and clr6 mutant cells, except at 2±3 foci near the nuclear periphery, presumably representing heterochromatic loci (Figure 7) ------- COMMENT: 669a6e624422bdf5 57 taJ02sTIhiiNEe9IoO4RsILBwGY We found that H3 Ser10 phosphorylation levels were considerably reduced in alp13, pst2 and clr6 mutant cells, except at 2±3 foci near the nuclear periphery, presumably representing heterochromatic loci (Figure 7) ------- COMMENT: 669b2243a78396bd 3 KJ5WCjBdZoKaAaJym8JRZOgEMsQ (comment: CHECK not sure if it is endo, exo, or both. It is def acting on DNA. More specific terms for DNA specify endo or exo.) ------- COMMENT: 669b2243a78396bd 5 f59AjvWekWCjQdU+G1GBcJJle1c (comment: CHECK not sure if it is endo, exo or both? so went with more general term) ------- COMMENT: 66a8c83f6f122a84 2 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: 66a8c83f6f122a84 3 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: 66a8c83f6f122a84 4 PF0/rezWsxQ4cOd2+QLqpqA4JMA Fig. 2c ------- COMMENT: 66a8c83f6f122a84 5 PF0/rezWsxQ4cOd2+QLqpqA4JMA Fig. 2c ------- COMMENT: 66a8c83f6f122a84 6 PF0/rezWsxQ4cOd2+QLqpqA4JMA Fig. 2c ------- COMMENT: 66a8c83f6f122a84 9 xzHBf2xhWepcJ8UFTdxE8qzATeQ fig 2 and 3d ------- COMMENT: 66a8c83f6f122a84 14 8fdlr09VWchtHu3iQQ57DGhYvVI Fig. 4a ------- COMMENT: 66a8c83f6f122a84 15 8fdlr09VWchtHu3iQQ57DGhYvVI Fig. 4a ------- COMMENT: 66a8c83f6f122a84 16 yw/6DnFvaJ7oyw7jm9xcw1F50+I Fig. 4c ------- COMMENT: 66a8c83f6f122a84 17 ZM0LVjtfYsCENue0wg1a0vPe8jk Fig. 4d,e ------- COMMENT: 66a8c83f6f122a84 18 yw/6DnFvaJ7oyw7jm9xcw1F50+I Fig. 4c ------- COMMENT: 66a8c83f6f122a84 20 yw/6DnFvaJ7oyw7jm9xcw1F50+I Fig. 4c ------- COMMENT: 66a8c83f6f122a84 21 NOecXQmUdKw3ue9XajAeXxyFvEA Fig. 4d ------- COMMENT: 66b7ec899d565a8b 1 Ci+bKhUitsutwHeHgf5TiETS96Y Fig1 ------- COMMENT: 66b7ec899d565a8b 2 dLMc2lN+y4Ufz2MhLOHEIjs6B2A Fig 2A ------- COMMENT: 66b7ec899d565a8b 3 dLMc2lN+y4Ufz2MhLOHEIjs6B2A Fig 2A ------- COMMENT: 66b7ec899d565a8b 4 dLMc2lN+y4Ufz2MhLOHEIjs6B2A Fig 2A ------- COMMENT: 66b7ec899d565a8b 18 lgoOLEhBWGwTySTF/NR4Pxj6B7A Fig5 Table 4 ------- COMMENT: 66b7ec899d565a8b 19 vCleNwE+UyaGDHMLt8+rI18iRRs Fig5 Table 3,4 ------- COMMENT: 66b7ec899d565a8b 20 mfqSbOJvicyHWmdYx1Ik90fu9y8 Fig 5, Table 3,4 ------- COMMENT: 66b7ec899d565a8b 21 VN4N+vW75GMJ++O3CfyecQbICco Fig6A ------- COMMENT: 66b7ec899d565a8b 22 VN4N+vW75GMJ++O3CfyecQbICco Fig6A ------- COMMENT: 66b7ec899d565a8b 23 VN4N+vW75GMJ++O3CfyecQbICco Fig6A ------- COMMENT: 66b7ec899d565a8b 24 OXqCw37/Uyk9HcquOWmbm+UAQCU Fig6C ------- COMMENT: 66b7ec899d565a8b 25 jVnlofAEw8ROw1ipdyPc/T0CQeU location is abolished during mating Fig4Dc and Fig4Ec ------- COMMENT: 66b7ec899d565a8b 26 OXqCw37/Uyk9HcquOWmbm+UAQCU Fig6C ------- COMMENT: 66b7ec899d565a8b 27 OXqCw37/Uyk9HcquOWmbm+UAQCU Fig6C ------- COMMENT: 66b7ec899d565a8b 32 FEVRq0o+6UBODOIIPV6ys97nMmk Fig8D,E pom1 has a role in the relocalisation of actin to the shmooing cell tip ------- COMMENT: 66b7ec899d565a8b 33 LOZdRNzIrVFgVhV6rIIJQi9HDPY After pheromone addition, Tea1GFP became mostly lost from the growing end and was redistributed along the cell periphery at the nongrowing larger end (Fig. 3E). After pheromone addition, Tea2GFP and Tip1YFP were also reduced at the growing end, accumulating at the non-growing end and in the cytoplasm, often as dots in a row (Fig. 3E). The same relocalisation was observed for Tea2GFP during an h90 mating. In conjugating cells, Tea2GFP was found to localise to the nongrowing ends with some dots in the.. ------- COMMENT: 66b7ec899d565a8b 34 LOZdRNzIrVFgVhV6rIIJQi9HDPY After pheromone addition, Tea1GFP became mostly lost from the growing end and was redistributed along the cell periphery at the nongrowing larger end (Fig. 3E). After pheromone addition, Tea2GFP and Tip1YFP were also reduced at the growing end, accumulating at the non-growing end and in the cytoplasm, often as dots in a row (Fig. 3E). The same relocalisation was observed for Tea2GFP during an h90 mating. In conjugating cells, Tea2GFP was found to localise to the nongrowing ends with some dots in the.. ------- COMMENT: 66b7ec899d565a8b 35 LOZdRNzIrVFgVhV6rIIJQi9HDPY After pheromone addition, Tea1GFP became mostly lost from the growing end and was redistributed along the cell periphery at the nongrowing larger end (Fig. 3E). After pheromone addition, Tea2GFP and Tip1YFP were also reduced at the growing end, accumulating at the non-growing end and in the cytoplasm, often as dots in a row (Fig. 3E). The same relocalisation was observed for Tea2GFP during an h90 mating. In conjugating cells, Tea2GFP was found to localise to the nongrowing ends with some dots in the.. ------- COMMENT: 66b7ec899d565a8b 36 eo5sBM1Wo5t+tplbhndfDoFJQfQ FIg 4 F location alkso exists during mating ------- COMMENT: 66b7ec899d565a8b 37 7ODNqoiVJggDHLb0HU0cTImcVF0 Fig6B All three mutants were able to detect and respond to pheromone by arresting in G1, as shown by FACS analysis (Fig. 6B), ------- COMMENT: 66b7ec899d565a8b 38 grAd6B7l6k+U9Mt0sU9mRy+4F+8 Fig6B ------- COMMENT: 66b7ec899d565a8b 39 grAd6B7l6k+U9Mt0sU9mRy+4F+8 Fig6B ------- COMMENT: 66b7ec899d565a8b 40 AtW0g/MawvJz0SrJnUCYNfaHmcg regulation of ------- COMMENT: 66bd776c94a56c16 1 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 66bd776c94a56c16 2 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 66bd776c94a56c16 3 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 66bd776c94a56c16 4 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 66bd776c94a56c16 5 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 66bd776c94a56c16 6 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 66bd776c94a56c16 7 VDN5sFCJhvYM6J/tU0KeDfKnwH8 Fig. 3B and D ------- COMMENT: 66bd776c94a56c16 8 VDN5sFCJhvYM6J/tU0KeDfKnwH8 Fig. 3B and D ------- COMMENT: 66bd776c94a56c16 9 VDN5sFCJhvYM6J/tU0KeDfKnwH8 Fig. 3B and D ------- COMMENT: 66bd776c94a56c16 10 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 66bd776c94a56c16 11 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 66bd776c94a56c16 12 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 66bd776c94a56c16 13 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 66bd776c94a56c16 14 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 66bd776c94a56c16 15 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 66bd776c94a56c16 16 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 66bd776c94a56c16 17 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 66bd776c94a56c16 18 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 66bd776c94a56c16 19 AsAW6ZfEAClJqO8BeIfqcJ+aVfE This hypersensitivity to LatB of mutants of either Drkp1 or Dpck2 indicates that both Rkp1 and Pck2 are required for stable filamentous actin network. ------- COMMENT: 66bd776c94a56c16 20 AsAW6ZfEAClJqO8BeIfqcJ+aVfE This hypersensitivity to LatB of mutants of either Drkp1 or Dpck2 indicates that both Rkp1 and Pck2 are required for stable filamentous actin network. ------- COMMENT: 66bd776c94a56c16 21 G85Ex4x2dsAxtvCNFV+upxltz3A As shown in Fig. 5, growth of the cells expressing human RACK1 was not seriously affected by the presence of LatB indicating human RACK1 is a functional homolog of Rkp1. ------- COMMENT: 66c9ead399a6a1ec 1 9HUxE9MZ7O9G4Gp0wr27+rzZV5c (comment: S2P form) ------- COMMENT: 66d0d6609144ac64 1 cw2FN+0wFdnp/fQfzkTjawR3+cU (comment: CHECK of human pyruvyltransferase activity for the LacNAc-pNP) ------- COMMENT: 66d0d6609144ac64 2 cw2FN+0wFdnp/fQfzkTjawR3+cU (comment: CHECK of human pyruvyltransferase activity for the LacNAc-pNP) ------- COMMENT: 66d1d84a63cd8a3e 1 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 66d1d84a63cd8a3e 2 G1QV0olEUiWBmGlAZqPkymnJrks Fig. 2 However, S. pombe is viable when Pro3 or Pro6 is changed to alanine in every other heptad, includ- ing the rump, in the context of the full-length CTD (Fig. 2), signifying that reduced proline content is tolerated and that Pro3 and Pro6 need not be present in consecutive heptads ------- COMMENT: 66d1d84a63cd8a3e 3 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 66d1d84a63cd8a3e 4 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 66d1d84a63cd8a3e 5 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 66d1d84a63cd8a3e 6 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 66d1d84a63cd8a3e 7 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 66d1d84a63cd8a3e 8 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 66d1d84a63cd8a3e 9 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 66d1d84a63cd8a3e 10 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 66d1d84a63cd8a3e 11 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 66d1d84a63cd8a3e 12 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 66d1d84a63cd8a3e 13 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 66d1d84a63cd8a3e 14 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 66d1d84a63cd8a3e 15 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 66d1d84a63cd8a3e 16 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 66d1d84a63cd8a3e 17 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 66d1d84a63cd8a3e 18 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 66d1d84a63cd8a3e 19 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 66d1d84a63cd8a3e 20 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 66d1d84a63cd8a3e 21 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 66d1d84a63cd8a3e 22 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 66d1d84a63cd8a3e 23 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 66d1d84a63cd8a3e 24 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 66d1d84a63cd8a3e 25 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 66d1d84a63cd8a3e 26 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 66d1d84a63cd8a3e 27 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 66d1d84a63cd8a3e 28 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 66d1d84a63cd8a3e 29 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 66d1d84a63cd8a3e 30 G1QV0olEUiWBmGlAZqPkymnJrks Fig. 2 However, S. pombe is viable when Pro3 or Pro6 is changed to alanine in every other heptad, includ- ing the rump, in the context of the full-length CTD (Fig. 2), signifying that reduced proline content is tolerated and that Pro3 and Pro6 need not be present in consecutive heptads ------- COMMENT: 66d1d84a63cd8a3e 31 7JzopYkOQHH2rAYbaAzUITJIliA (comment: CHECK Need to add modified version) ------- COMMENT: 66ec44c3d78cc17b 1 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 66ec44c3d78cc17b 2 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 66ec44c3d78cc17b 3 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 66ec44c3d78cc17b 4 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 66ec44c3d78cc17b 5 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 66ec44c3d78cc17b 6 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 66ec44c3d78cc17b 7 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 66ec44c3d78cc17b 8 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 66ec44c3d78cc17b 9 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 66ec44c3d78cc17b 10 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 66ec44c3d78cc17b 11 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 66ec44c3d78cc17b 12 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 66ec44c3d78cc17b 13 uQgbw7J9ABjaRCEtM09cOzOzPqY As shown in Fig. 1a, Δppr2 cells progressively lost viability during growth in YES medium (Fig. 1a) ------- COMMENT: 66ec44c3d78cc17b 14 ceFf4ZKiUXiB4IeGfVt0zd0hrX4 Deletion of ppr2 also increased the sensitivity of cells to ferrous iron (Fe2+) and ferric iron (Fe3+) (Fig. 1b). ------- COMMENT: 66ec44c3d78cc17b 15 0+CIgkPujArAc9l76/YyrXvsPGE + Fe2(SO4)3 Deletion of ppr2 also increased the sensitivity of cells to ferrous iron (Fe2+) and ferric iron (Fe3+) (Fig. 1b). ------- COMMENT: 66ec44c3d78cc17b 17 EiyXhTQtDfimxTwXIiJ3EONiUFg We found that DFO significantly increases the viability of Δppr2 cells (Fig. 1c). (normal compared to WT) ------- COMMENT: 66ec44c3d78cc17b 18 nPWl5A0QA3UDK1ILulm3/0a4loY Increased lipid peroxidation was detected in Δppr2 cells ------- COMMENT: 66ec44c3d78cc17b 19 nPWl5A0QA3UDK1ILulm3/0a4loY Increased lipid peroxidation was detected in Δppr2 cells ------- COMMENT: 66ec44c3d78cc17b 20 dNUDpsdo3Uj5GzVy5kXY3vb3B4Y However, DFO could not enables growth of Δppr2 cells on glycerol medium, which requires mitochondrial respiration, suggesting that DFO could not rescue of the respiratory growth defect of Δppr2 cells (Fig. 1d) ------- COMMENT: 66ec44c3d78cc17b 23 00kDiN0znLTthP9LbGJZDRnJyaA However, deletion of frp1 could not rescue respira- tory growth defect in Δppr2 cells as Δppr2Δfrp1 could not grow on glycerol medium (Fig. 4b). ------- COMMENT: 66ec44c3d78cc17b 30 dNUDpsdo3Uj5GzVy5kXY3vb3B4Y However, DFO could not enables growth of Δppr2 cells on glycerol medium, which requires mitochondrial respiration, suggesting that DFO could not rescue of the respiratory growth defect of Δppr2 cells (Fig. 1d) ------- COMMENT: 670e6db747c0dacb 7 PnZWdOVaaA8HcJFo2iiuYl66h8k binucleate fypo/issues/#2400 fypo/issues/#2401 ------- COMMENT: 6710099b122fb72a 11 5DWqo03puFeQtodWqcCbmJ6nfyM fig 1A ------- COMMENT: 6710099b122fb72a 13 IR6+oG7T4QSpwJ+y9BSgLISsAdU fig 1A (comment: CHECK during cytokinesis) ------- COMMENT: 6710099b122fb72a 14 jHJoBGeWfm+rY5t7k/8VlRIxkZY fig 1B (comment: CHECK swollen multiseptate elongated) ------- COMMENT: 6710099b122fb72a 15 LkGQQw8FQBPnHuF6zSR3Jquhnkc fig 1C ------- COMMENT: 6710099b122fb72a 16 LkGQQw8FQBPnHuF6zSR3Jquhnkc fig 1C ------- COMMENT: 6710099b122fb72a 17 LkGQQw8FQBPnHuF6zSR3Jquhnkc fig 1C ------- COMMENT: 6710099b122fb72a 18 GC3M74I6VP4IUx9ePVUTKrwPIh4 fig 1FG ------- COMMENT: 6710099b122fb72a 19 GC3M74I6VP4IUx9ePVUTKrwPIh4 fig 1FG ------- COMMENT: 6710099b122fb72a 20 GC3M74I6VP4IUx9ePVUTKrwPIh4 fig 1FG ------- COMMENT: 6710099b122fb72a 21 aCIPxq97hBAm6OV0YQrh9YCur7c fig 1FG slides along axis from midpoint ------- COMMENT: 6710099b122fb72a 22 4oQlUUq2rcPaKwNNOwtKM57xzMo Fig 2 A ------- COMMENT: 6710099b122fb72a 23 4oQlUUq2rcPaKwNNOwtKM57xzMo Fig 2 A ------- COMMENT: 6710099b122fb72a 24 4oQlUUq2rcPaKwNNOwtKM57xzMo Fig 2 A ------- COMMENT: 6710099b122fb72a 25 2fGqhme4LjpZI8oxONLjck5pnr4 Fig 2 A (comment: 2x WT) ------- COMMENT: 6710099b122fb72a 26 yBfrcASLKMfTiH5/CGrWowRRHLM Fig 2 A (comment: 3x WT) ------- COMMENT: 6710099b122fb72a 28 joC6jJccwRwyV1DTiwAjoKSRPSQ Fig S3B ------- COMMENT: 6710099b122fb72a 29 joC6jJccwRwyV1DTiwAjoKSRPSQ Fig S3B ------- COMMENT: 6710099b122fb72a 30 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 6710099b122fb72a 31 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 6710099b122fb72a 32 SoUD4Us9ZDdo1HsdEEY7zxpwMNU Fig S4D S4E ------- COMMENT: 6710099b122fb72a 33 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 6710099b122fb72a 34 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 6710099b122fb72a 35 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 6710099b122fb72a 36 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 6710099b122fb72a 37 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 6743693a29a7b5bb 1 aSH1UoHehaOSoogIzECd67T4pn4 figure 1 ------- COMMENT: 6743693a29a7b5bb 2 orAxYi8DK1Adj43/rDjYPjPg3CU figure 1 G/H (comment: from preexisting microtubules) ------- COMMENT: 6743693a29a7b5bb 3 kMBL1O7e1mc5rsznrZoIMYzI3dA figure 2A (comment:CHECK with increased localization to SPB) ------- COMMENT: 6743693a29a7b5bb 4 Dygvz0IEwP6twsKHK/jsDJvx/Qc figure 2B ------- COMMENT: 6743693a29a7b5bb 5 kMBL1O7e1mc5rsznrZoIMYzI3dA figure 2A (comment:CHECK with increased localization to SPB) ------- COMMENT: 6743693a29a7b5bb 6 Dygvz0IEwP6twsKHK/jsDJvx/Qc figure 2B ------- COMMENT: 6743693a29a7b5bb 7 Dygvz0IEwP6twsKHK/jsDJvx/Qc figure 2B ------- COMMENT: 6743693a29a7b5bb 8 gzQcytmgkoqEDIkK+b9+9fWHCz8 Therefore, we concluded that Rsp1 is required to prevent excessive accumulation of Mto1 ------- COMMENT: 6743693a29a7b5bb 10 H0MnaHI1FiMjP1CYNeqjyYIN72Q figure 5A ------- COMMENT: 6743693a29a7b5bb 11 H0MnaHI1FiMjP1CYNeqjyYIN72Q figure 5A ------- COMMENT: 6743693a29a7b5bb 12 9YBUs/tUh7WU3Ff4L/Nho4GvmR4 fig 5A (comment:CHECK recruitment) ------- COMMENT: 6743693a29a7b5bb 13 4LVWkJMB5TzrpnGu1RNmHgsFk8g figure 5GH ------- COMMENT: 6743693a29a7b5bb 14 gzQcytmgkoqEDIkK+b9+9fWHCz8 Therefore, we concluded that Rsp1 is required to prevent excessive accumulation of Mto1 ------- COMMENT: 6743693a29a7b5bb 15 /RO0n6iL6M4dipm2moDG11eE8ak figure 5I ------- COMMENT: 6743693a29a7b5bb 16 5RAPOy3VFV1sT+hUk9zEF78F1xc figure 6D ------- COMMENT: 6743693a29a7b5bb 17 BpCcqlUo1AzV5Xd7O8/QyphG68A figure 6AC ------- COMMENT: 6743693a29a7b5bb 18 BpCcqlUo1AzV5Xd7O8/QyphG68A figure 6AC ------- COMMENT: 6788fbad2542709a 10 +rrxKFdNIO3dkxNXsh45IAHiHVs Fig 5B. Septation start is delayed when the function of Sid2 is compromised. Cells were grown in YES at 25C, shifted to 28C for 4 h and imaged as in Fig 1. The data are developed in Table 1 and Table 3. ------- COMMENT: 6788fbad2542709a 11 6pal6arNP29ZIc3VBJP8lREu9g0 Fig S3E. The timing of septation onset depends on the AR function. The start of septation is delayed when the function of the AR F-BAR protein Cdc15 is compromised. ------- COMMENT: 6788fbad2542709a 12 QQ8tWnOHqm6/TZaLoGY4syK7FJo Fig S3D. The timing of septation onset depends on the AR function. The start of septation is delayed when the function of the AR unconventional type II myosin Myp2 is compromised. ------- COMMENT: 6788fbad2542709a 13 oz5XHlthf0rO8o0qd2oPUoOMDSg Fig 2. The start of septation scales with anaphase B progression and cell size in fission yeast and correlates linearly with the cell length. ------- COMMENT: 6788fbad2542709a 14 JkBY+CZUTdDYYniyNdEPH3tl2+o Fig 2. The start of septation scales with anaphase B progression and cell size in fission yeast. and correlates linearly with the cell length. ------- COMMENT: 6788fbad2542709a 15 oz5XHlthf0rO8o0qd2oPUoOMDSg Fig 2. The start of septation scales with anaphase B progression and cell size in fission yeast and correlates linearly with the cell length. ------- COMMENT: 6788fbad2542709a 16 gQZhLxQkU2vhMteERPdslswA940 Fig 2. The start of septation scales with anaphase B progression and correlates linearly with the cell length. ------- COMMENT: 6788fbad2542709a 17 uBPwLtPxYhgjABHLhdeMOaM5dIw Fig 4E and F. Inactivation of Cdc2 kinase in early mitosis induces a very premature septation onset. ATP-analogue sensitive cdc2-asM17 mutant cells carrying Cdc13-GFP were G2-arrested by growth in the presence of 1 μM1-NP-PP1 for 3.5 h at 32C. Then, the cells were G2-released by transfer to a fresh medium and imaged to detect the entry into mitosis. Cdc2 was inactivated during early mitosis transferring the cells to a fresh medium containing either DMSO or 10 μM1-NP-PP1. ------- COMMENT: 6788fbad2542709a 19 vIvx2GFOe15BN7gLiuePEZmqOMo Fig 5E. Timely activation of septum synthesis does not depend on SIN asymmetry. Defective SIN-Inhibitory Phosphatase (SIP) complex csc2Δ cells were examined. The data of cells of C, D and E are developed in S2 Table. ------- COMMENT: 6788fbad2542709a 20 tVn6zbvCrpas//AcFAseKtaeGWc Fig 6. The levels of Etd1 and Rho1 regulate the timing of septation start. (A) The timing of septum deposition onset correlates with the start of increase of Etd1 in the cell middle. Cells were grown inMMwithout thiamine (GFP-etd1+ induced) at 25ÊC for 24 h and imaged as in Fig 1. (B) The increase of Etd1 in the cell middle and concomitant initiation of septation are delayed in long cells. Cells were analyzed as in A after 3.5 h of cell cycle arrest at 36ÊC. Graphs to the right show the total fluorescence of GFP-Etd1 at the cell poles and middle in the series to the left. A.U., arbitrary units. Arrow, cortical localization of Etd1 in the cell middle. Dashed outlines indicate the ROIs used to measure the total fluorescence of GFP-Etd1 in the corresponding regions of the cell. (C) The timing of septation onset is dependent on the level of etd1+. Cells expressing endogenous etd1+ and 41X-GFP-etd1+ grown at 32ÊC for 24 h either in the absence (ON, high etd1+ level; n = 4, 34 cells) or in the presence of thiamine (OFF, wild-type etd1+ level; n = 2, 11 cells), and cells expressing etd1Δ 81X-etd1+ grown for 15 h with thiamine (OFF, very low etd1+ level; n = 3, 23 cells), just before the emergence of SIN phenotype were analyzed as in A. ------- COMMENT: 6788fbad2542709a 21 tVn6zbvCrpas//AcFAseKtaeGWc Fig 6. The levels of Etd1 and Rho1 regulate the timing of septation start. (A) The timing of septum deposition onset correlates with the start of increase of Etd1 in the cell middle. Cells were grown inMMwithout thiamine (GFP-etd1+ induced) at 25ÊC for 24 h and imaged as in Fig 1. (B) The increase of Etd1 in the cell middle and concomitant initiation of septation are delayed in long cells. Cells were analyzed as in A after 3.5 h of cell cycle arrest at 36ÊC. Graphs to the right show the total fluorescence of GFP-Etd1 at the cell poles and middle in the series to the left. A.U., arbitrary units. Arrow, cortical localization of Etd1 in the cell middle. Dashed outlines indicate the ROIs used to measure the total fluorescence of GFP-Etd1 in the corresponding regions of the cell. (C) The timing of septation onset is dependent on the level of etd1+. Cells expressing endogenous etd1+ and 41X-GFP-etd1+ grown at 32ÊC for 24 h either in the absence (ON, high etd1+ level; n = 4, 34 cells) or in the presence of thiamine (OFF, wild-type etd1+ level; n = 2, 11 cells), and cells expressing etd1Δ 81X-etd1+ grown for 15 h with thiamine (OFF, very low etd1+ level; n = 3, 23 cells), just before the emergence of SIN phenotype were analyzed as in A. ------- COMMENT: 6788fbad2542709a 23 EaYibb6d16phJIZwRyCFAt5qShg Fig 6. The levels of Etd1 and Rho1 regulate the timing of septation start. (F) The start of septum deposition is dependent on the level of rho1+. Cells expressing endogenous rho1+ and 3Xrho1+ grown at 32ÊC for 16 h either without (ON, high rho1+ level; n = 2, 14 cells) or with thiamine (OFF, wild-type rho1+ level; n = 2, 16 cells) were analyzed. ------- COMMENT: 6788fbad2542709a 24 hbiBqa65J1+wdt0Yn6ZWX5wk91I Fig 7. The spindle and the proximity of the nucleus to the division site are required for proper septum synthesis activation in fission yeast. (A) Scheme of the steps required to prevent nuclear separation and to maintain or separate the undivided nucleus from the cell middle and/or from the division site. (A-1 and C) Nucleus and division site are maintained in the cell middle; cells were treated for 90 min and imaged with methyl 2-benzimidazolecarbamate (or carbendazim, MBC, 50 μg ml-1) to avoid spindle assembly and nuclear separation. (A-2 and D) Nucleus and division site are relocated to a cell end; cells were treated for 45 min, centrifuged to displace the nucleus, treated 45 more min and visualized with MBC. (A-3 and E) The nucleus is relocated and separated from the division plane; cells were treated for 90 min, centrifuged and examined with MBC. (B) mad2Δ cells were grown and imaged without MBC as in Fig 1. The mad2Δ cells were used to avoid a delay caused by the activation of the spindle assembly checkpoint. (C-E) The premature and uncoupled septation start caused by the absence of the spindle depends on the position of the nucleus. mad2Δ cells were processed as in A to prevent nuclear separation and to maintain or separate the undivided nucleus from the cell middle and/or the division site. (C) The nucleus and division site are maintained in the cell middle. (F) The nucleus and division site are relocated to a cell end. (E) The nucleus is relocated and separated from the division plane. MBC-treated cells were imaged as in B. Anaphase A onset was considered as time zero. Graphs to the right are as in Fig 4. Dashed lines and arrowheads: green, anaphase A onset; dark blue, septum synthesis start; light blue, septum ingression onset. White arrowhead: first CW-stained septum synthesis detection. White arrow: first CW-staining increase showing septum ingression. A.U., arbitrary units. (F) Uncoupled septum synthesis and ingression timing with MBC is restored to wild-type levels when the undivided nucleus is separated from the division site. Table showing the time between anaphase A (green) and septum synthesis start (dark blue) or septum ingression onset (light blue) in the indicated cells. Parenthesis: n, number of experiments and cells; T, delay in septum synthesis and ingression start with respect to control cells with MBC as in C. ------- COMMENT: 6788fbad2542709a 25 dt2jB2m7nFTsBG9ZljG1IC18A3E Fig 7. The spindle and the proximity of the nucleus to the division site are required for proper septum synthesis activation in fission yeast. (A) Scheme of the steps required to prevent nuclear separation and to maintain or separate the undivided nucleus from the cell middle and/or from the division site. (A-1 and C) Nucleus and division site are maintained in the cell middle; cells were treated for 90 min and imaged with methyl 2-benzimidazolecarbamate (or carbendazim, MBC, 50 μg ml-1) to avoid spindle assembly and nuclear separation. (A-2 and D) Nucleus and division site are relocated to a cell end; cells were treated for 45 min, centrifuged to displace the nucleus, treated 45 more min and visualized with MBC. (A-3 and E) The nucleus is relocated and separated from the division plane; cells were treated for 90 min, centrifuged and examined with MBC. (B) mad2Δ cells were grown and imaged without MBC as in Fig 1. The mad2Δ cells were used to avoid a delay caused by the activation of the spindle assembly checkpoint. (C-E) The premature and uncoupled septation start caused by the absence of the spindle depends on the position of the nucleus. mad2Δ cells were processed as in A to prevent nuclear separation and to maintain or separate the undivided nucleus from the cell middle and/or the division site. (C) The nucleus and division site are maintained in the cell middle. (F) The nucleus and division site are relocated to a cell end. (E) The nucleus is relocated and separated from the division plane. MBC-treated cells were imaged as in B. Anaphase A onset was considered as time zero. Graphs to the right are as in Fig 4. Dashed lines and arrowheads: green, anaphase A onset; dark blue, septum synthesis start; light blue, septum ingression onset. White arrowhead: first CW-stained septum synthesis detection. White arrow: first CW-staining increase showing septum ingression. A.U., arbitrary units. (F) Uncoupled septum synthesis and ingression timing with MBC is restored to wild-type levels when the undivided nucleus is separated from the division site. Table showing the time between anaphase A (green) and septum synthesis start (dark blue) or septum ingression onset (light blue) in the indicated cells. Parenthesis: n, number of experiments and cells; T, delay in septum synthesis and ingression start with respect to control cells with MBC as in C. ------- COMMENT: 6788fbad2542709a 26 xmZYgdvvzTZR5kitp7xCF/oDFvw S5 Fig. The establishment of SIN asymmetry and the timely activation of septum synthesis do not depend on each other. (A, B) Early log-phase wild-type and thermosensitive cps1-191 (Bgs1) mutant cells were grown in YES at 25ÊC, shifted to 28ÊC for 1 h (A) or 32ÊC for 30 min (B) to produce a gradual delay in the onset of septum synthesis of cps1-191 mutant, and imaged as in Fig 5C. Anaphase B onset is considered as time zero (T = 0). White arrow: first CWstained detection of septum synthesis. Arrowheads: green, anaphase B onset; blue, septum deposition start (time immediately before septum detection with CW); red, complete asymmetry of SIN Cdc7, being Cdc7-GFP completely lost from one SPB. The data of this figure are developed in S2 Table. (C) The timing of septation onset is not related to the asymmetry of SIN. Early log-phase wild-type cells were grown in YES at 25ÊC, 28ÊC or 32ÊC, imaged as in Fig 5C and the timings of SIN asymmetry and of septation onset were determined with respect to the anaphase B onset (see also the data in S2 Table). ------- COMMENT: 6788fbad2542709a 27 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 6788fbad2542709a 28 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 6788fbad2542709a 29 YoEnt64v4u12felLFPYzRX0wxPI Fig 4D & E ------- COMMENT: 6788fbad2542709a 30 6Dnol0+1TbDsEACmYeN9phA5Wuk Fig 5B, Table 1; (comment: also inferred from localization timing) ------- COMMENT: 6788fbad2542709a 31 nDvr6BcAJAvr3DMhKkvyRvA3OsU Fig 56A-D; (comment: also inferred from localization timing) ------- COMMENT: 6788fbad2542709a 32 I13mBjeo0D/e+aRXX6aGk5LE4YE Fig 56E; (comment: also inferred from localization timing) ------- COMMENT: 67ba83cbaba024a7 48 JBQwJgbRBR+dlg5jsdWChWv95aE (comment: yrosine; residue not determined) ------- COMMENT: 67c7ee73b6bc39ab 1 YfNMk50W4IYYn0jAwi12M2Ps9wo Cdc2 phosphorylates Rap1 at Thr378, Ser422, and Ser513 during M phase. Ser456 of Rap1 is also phosphorylated during M phase by an unknown kinase. Ser213 of Rap1 is phosphorylated throughout the cell cycle. These phosphorylations are required for the efficient detachment of telomeres from the nuclear envelope. ------- COMMENT: 67c7ee73b6bc39ab 6 YfNMk50W4IYYn0jAwi12M2Ps9wo Cdc2 phosphorylates Rap1 at Thr378, Ser422, and Ser513 during M phase. Ser456 of Rap1 is also phosphorylated during M phase by an unknown kinase. Ser213 of Rap1 is phosphorylated throughout the cell cycle. These phosphorylations are required for the efficient detachment of telomeres from the nuclear envelope. ------- COMMENT: 67c7ee73b6bc39ab 12 bYLaZDVV3Kr1wa9BZxwysoy6BU0 cdc2 phosphorylates rap1. phosphorylated rap1 binds bqt4 less efficiently. rap1-bqt4 binding is required for telomere tethering at nuclear periphery. In WT cells telomere tehtering is abolished during M phase. ------- COMMENT: 67c7ee73b6bc39ab 30 ETXUwrrPBMDRsUWfxFfWB4NW8q4 (comment: at Ser/Thr-Pro site) ------- COMMENT: 67f41f34003a2a4c 20 xX7x40EqeCwDcm5/7gukUh2aGPw (comment: they form parts that fail to mature) ------- COMMENT: 67f8ea3e5c1c1bfc 1 4Th/I6efUdR2DQFlnXUGvD5azdw Figue 1C ------- COMMENT: 67f8ea3e5c1c1bfc 2 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 67f8ea3e5c1c1bfc 3 4zEnbmM3DK49mCadtQW/g5pJnhs (DNS) ------- COMMENT: 67f8ea3e5c1c1bfc 4 uG8lotBx/q8WvbEL7OakITOms9k (DNS) ------- COMMENT: 67f8ea3e5c1c1bfc 5 uG8lotBx/q8WvbEL7OakITOms9k (DNS) ------- COMMENT: 67f8ea3e5c1c1bfc 6 KkFwFpgbEWpwhUqI9P43Zhsf/g8 Figure 1A (comment: vw changed to increased with low serverity as we compare to WT) ------- COMMENT: 67f8ea3e5c1c1bfc 13 1b4WycPpAcpZqhkd3G6a2UQAEEw (comment: similar to wt) ------- COMMENT: 67f8ea3e5c1c1bfc 15 vkeg6R6pUVaE4V0y1TCwiIMuGgY Although seb1- G476S asp1-H397A cells grew slowly in liquid medium at 30 °C, an assay of acid phosphatase showed that the double mutant expressed threefold higher levels of Pho1 than the seb1-G476S single mutant (Fig. 6B). (NOTE ABOUT 20 FOLD HIGHE TTHAN WT) ------- COMMENT: 67f8ea3e5c1c1bfc 16 Xk+RZCbQwWMtT3e63RX4knNAXLY ------- COMMENT: 67f8ea3e5c1c1bfc 17 yOvz34V9GfJT8yMXEUbIREkqrEQ (comment: compared to WT) ------- COMMENT: 67f8ea3e5c1c1bfc 18 yOvz34V9GfJT8yMXEUbIREkqrEQ (comment: compared to WT) ------- COMMENT: 67f8ea3e5c1c1bfc 19 yOvz34V9GfJT8yMXEUbIREkqrEQ (comment: compared to WT) ------- COMMENT: 67f8ea3e5c1c1bfc 20 gpHwLjEI9NcEfhstAQ8i1yKB00A Fig 7 (comment: compared to WT) ------- COMMENT: 67f8ea3e5c1c1bfc 21 gpHwLjEI9NcEfhstAQ8i1yKB00A Fig 7 (comment: compared to WT) ------- COMMENT: 67f8ea3e5c1c1bfc 22 MjUT/vcWhvJezDMoNKBkE578vLo rhn1∆ ts growth phenotype at 37ºC rescued by seb1-G476S ------- COMMENT: 67f8ea3e5c1c1bfc 23 b0HUV8ycSfmU9Nm1VjuoBAsEyfA ppn1∆ seb1-G476S cs growth phenotype at 25ºC ------- COMMENT: 67f8ea3e5c1c1bfc 24 KbwxcDUMgI/xWxIsAzLOGN1Tj3Q swd22∆ seb1-G476S cs growth phenotype at 25ºC ------- COMMENT: 67f8ea3e5c1c1bfc 25 H51xjCczBwJkfHPUH1O5WYleqUs (comment: CHECK seb1-G476S, aps1∆) ------- COMMENT: 67f8ea3e5c1c1bfc 26 TRkHW6XcSpBQAPeciSdOvt3PxBY (comment: CHECK (comment: CHECKseb1-G476S, rpb1-CTD-S7A)) ------- COMMENT: 67f8ea3e5c1c1bfc 27 UnhBguvEpGUHFg5eeLGzGJUubkw (comment: CHECK seb1-G476S, rpb1-S5•S5A(alternating CTD S5 residues mutated to Ala) ------- COMMENT: 67f8ea3e5c1c1bfc 28 bMMHtZzx8W+9B27znxdB6NWZtPA (comment: CHECK seb1-G476S, rpb1-P6•P6A(alternating CTD P6 residues mutated to Ala) ------- COMMENT: 67f8ea3e5c1c1bfc 29 VsGWOYVTEGA6dPj5b0bbWI3Q9Xo (comment: CHECK seb1-G476S, asp1-H397A) ------- COMMENT: 67f8ea3e5c1c1bfc 30 y6Wu1KDhdKainnC1cM7pEOlY/W4 (comment: CHECK seb1-G476S, asp1-D333A) ------- COMMENT: 67f8ea3e5c1c1bfc 31 0ePOaIRmqBQ4MQED/CyTxT8LMe8 (comment: CHECKseb1-G476S, asp1∆) ------- COMMENT: 67f8ea3e5c1c1bfc 32 by69c25akh0OgtcMlw4Aku0hbTI Fig 7a ------- COMMENT: 67f8ea3e5c1c1bfc 33 tc3rJxZshAX+tmBq7BR9TkADO4o Fig 7A ------- COMMENT: 67f8ea3e5c1c1bfc 34 tc3rJxZshAX+tmBq7BR9TkADO4o Fig 7A ------- COMMENT: 67f8ea3e5c1c1bfc 35 HSbI03SFtkcK6MT5+2DmNW9xBNw ( (comment: RNA-seq poly(A) tail reads). (Figs. 3–5 collectively fortify the case for seb1-G476S as a gain-of-function mutation in Seb1 that elicits precocious lncRNA termination dependent on lncRNA PAS and cleavage/polyadenylation factors.) ------- COMMENT: 67f8ea3e5c1c1bfc 36 rUZ2TZTibFtCpRbzeDvIZquDcIc ------- COMMENT: 67f8ea3e5c1c1bfc 38 Ocx+iB/95EBAbk5q7f3QrSYRqPw Figure 1c ------- COMMENT: 67f8ea3e5c1c1bfc 41 Xk+RZCbQwWMtT3e63RX4knNAXLY ------- COMMENT: 67f8ea3e5c1c1bfc 42 Xk+RZCbQwWMtT3e63RX4knNAXLY ------- COMMENT: 67f8ea3e5c1c1bfc 43 Xk+RZCbQwWMtT3e63RX4knNAXLY ------- COMMENT: 67f8ea3e5c1c1bfc 44 Xk+RZCbQwWMtT3e63RX4knNAXLY ------- COMMENT: 67f8ea3e5c1c1bfc 45 Xk+RZCbQwWMtT3e63RX4knNAXLY ------- COMMENT: 67f8ea3e5c1c1bfc 46 SDORamgjP8m4ECi97mGOaXtfQgg (comment: CHECK seb1-1, rpb1-S5•S5A alternating CTD S5 residues mutated to Ala) ------- COMMENT: 67f8ea3e5c1c1bfc 47 ZUJqfDsXJCWqHXgWoc9XwNMrqoE (comment: CHECK seb1-1, rpb1-P6•P6A alternating CTD P6 residues mutated to Ala) ------- COMMENT: 67f8ea3e5c1c1bfc 48 W0dIOjew4K4O1lACw3DOooDRx4E (comment: CHECK seb1-1, rpb1-CTD-S2A) ------- COMMENT: 67f8ea3e5c1c1bfc 49 WFEDg+E5m4LSOnnDTR72D8f0suk (comment: CHECK seb1-1, ctf1∆) ------- COMMENT: 67f8ea3e5c1c1bfc 50 OhQtr1S+oYeg47KPC0pvHzarb4U (comment: CHECK seb1-1, ssu72-C13S) ------- COMMENT: 67f8ea3e5c1c1bfc 51 zawsF45xSVspGE+2hFxjBS1RmUg (comment: CHECK seb1-1 ts growth phenotype at 37ºC rescued by dis2∆ ------- COMMENT: 67f8ea3e5c1c1bfc 52 5SDTvplHo+NBQ8QNkLBgNCe5Ubk (comment: CHECK dis2∆ seb1-1 cs growth phenotype at 25-20ºC) ------- COMMENT: 67f8ea3e5c1c1bfc 53 Wi3HnZzZun7WdRZs7pJ31u2JE50 (comment: CHECK seb1-1 ts growth phenotype at 37ºC rescued by rpb1-CTD-S7A) ------- COMMENT: 67f8ea3e5c1c1bfc 54 ojpnwrTDjNWrUZx5wPqZU4V2wWs (comment: CHECK ppn1∆ seb1-1 synthetic lethal at 25-20ºC) ------- COMMENT: 67f8ea3e5c1c1bfc 55 SydJPICHJ9JDXuizLtfYj4Htx0g (comment: CHECK swd22∆ seb1-1 synthetic lethal at 25-20ºC) ------- COMMENT: 67f8ea3e5c1c1bfc 57 7KDO7w/z187a6ydnFsKJOIj9Xbg Figue 3A Notable findings were that seb1-G476S rescued the ts growth defect of rhn1Δ at 37 °C...... ------- COMMENT: 67f8ea3e5c1c1bfc 59 1ql5vxcQogsKBW0z2EWi0JkTjcM ..... while rhn1Δ rescued the cs growth defect of seb1-G476S at 20 °C ------- COMMENT: 67f8ea3e5c1c1bfc 61 WC23V6Zq7g+trmJzvYRROx29kAA (Fig. 4B), Pho1 expression from the wild-type plasmid was increased sevenfold in seb1-G476S cells versus seb1-WT cells thereby echoing the derepressive effect of seb1-G476S on pho1 expression from the chromosomal prt–pho1 locus. ------- COMMENT: 67f8ea3e5c1c1bfc 62 PRX0p9PEQLKV15/IN7Ctapa9bkM Here, we found that tgp1 promoter–driven acid phosphatase expression was increased 30-fold in seb1-G476S cells versus seb1-WT cells and that this derepression was effaced by mutating the promoter-proximal nc- tgp1 PAS (Fig. 4D). ------- COMMENT: 67f8ea3e5c1c1bfc 63 aqewrAiAL65qGTdEmmSPes4JfgM The seb1-G476S and aps1Δ alleles were synthetically lethal; ------- COMMENT: 67f8ea3e5c1c1bfc 64 Xk+RZCbQwWMtT3e63RX4knNAXLY ------- COMMENT: 67f8ea3e5c1c1bfc 65 OIsbtpYwcR6yhVmNnpTQlTJMJsk ------- COMMENT: 682113d8f12b3fe4 1 mp0M7zDIFggn53e2MCluP++FNgs Fig. 5E ------- COMMENT: 682113d8f12b3fe4 2 fogVowcObkoWi79lNpy/RoRGREw Fig. 2G ------- COMMENT: 682113d8f12b3fe4 3 9Fc8tXV9RNdcZv/15EkP0jQOt00 Accordingly, Bub3 and Mad3 localized at kinetochores in a manner identical to Bub1 in spc7-12A and spc7-12E cells, ------- COMMENT: 682113d8f12b3fe4 4 9Fc8tXV9RNdcZv/15EkP0jQOt00 Accordingly, Bub3 and Mad3 localized at kinetochores in a manner identical to Bub1 in spc7-12A and spc7-12E cells, ------- COMMENT: 682113d8f12b3fe4 5 dCHNS3qJKkP38s/Yd3779Em3qe0 Fig. 1B This Bub1 enrichment is diminished in the spc7-23 mutant at a restrictive temperature24 (Fig. 1b). ------- COMMENT: 682113d8f12b3fe4 6 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 682113d8f12b3fe4 7 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 682113d8f12b3fe4 8 yoDajcQxac1S8UZ5vYpgU80P4Lk Strikingly, in spc7-12E cells, Bub1 localized at kinetochores throughout the entire cell cycle. Fig. 2H, I and S3C ------- COMMENT: 682113d8f12b3fe4 9 oQ24g6VFVa7MFs98eWbUbk5jzMQ However, the coexpression of Bub1 and Bub3 enabled this complex to interact with Spc7-12E. Fig. 4C ------- COMMENT: 682113d8f12b3fe4 10 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 682113d8f12b3fe4 15 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 682113d8f12b3fe4 16 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 682113d8f12b3fe4 17 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 682113d8f12b3fe4 18 mp0M7zDIFggn53e2MCluP++FNgs Fig. 5E ------- COMMENT: 682113d8f12b3fe4 19 sP8KzabzUEQjBg3uyC4T8bcCfUE The expression of this fusion protein impairs normal cell growth because of robust SAC activation (Supplementary Fig. S1b,c) ------- COMMENT: 682113d8f12b3fe4 22 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 682113d8f12b3fe4 23 XAWxsUqVCoJkh2k9D4NOPxRPfEk Moreover, fission yeast Mph1 (MPS1 homologue), which also localizes to kinetochores only at prometaphase (Supplementary Fig. S1a and Fig. 1D) ------- COMMENT: 682113d8f12b3fe4 24 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 682113d8f12b3fe4 25 GE55OyOOn5chuUI4w/hfesw2bkE Thus, Thr 77 and the MELT repeats in Spc7 are in vitro target sites of Mph1 kinase. Fig. 2 ------- COMMENT: 682113d8f12b3fe4 26 gLHEZS/RUqaKaX4QPSHqLuBpelQ Fig. 2I ------- COMMENT: 682113d8f12b3fe4 27 gLHEZS/RUqaKaX4QPSHqLuBpelQ Fig. 2I ------- COMMENT: 682113d8f12b3fe4 28 gLHEZS/RUqaKaX4QPSHqLuBpelQ Fig. 2I ------- COMMENT: 682113d8f12b3fe4 29 gLHEZS/RUqaKaX4QPSHqLuBpelQ Fig. 2I ------- COMMENT: 682113d8f12b3fe4 30 gLHEZS/RUqaKaX4QPSHqLuBpelQ Fig. 2I ------- COMMENT: 682113d8f12b3fe4 31 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 682113d8f12b3fe4 32 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 682113d8f12b3fe4 33 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 682113d8f12b3fe4 34 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 682113d8f12b3fe4 35 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 682113d8f12b3fe4 36 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 682113d8f12b3fe4 37 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 682113d8f12b3fe4 38 ScJ0ZXmZ1vEnvCpj/RACKed+C9g Fig. 5A. ------- COMMENT: 682113d8f12b3fe4 39 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 682113d8f12b3fe4 40 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 682113d8f12b3fe4 41 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 682113d8f12b3fe4 42 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 682113d8f12b3fe4 43 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 682113d8f12b3fe4 44 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 682113d8f12b3fe4 45 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 682113d8f12b3fe4 46 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 682113d8f12b3fe4 47 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 682113d8f12b3fe4 48 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 682113d8f12b3fe4 49 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 682113d8f12b3fe4 50 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 682113d8f12b3fe4 51 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 682113d8f12b3fe4 52 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 682113d8f12b3fe4 53 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 682113d8f12b3fe4 54 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 682113d8f12b3fe4 55 mp0M7zDIFggn53e2MCluP++FNgs Fig. 5E ------- COMMENT: 682113d8f12b3fe4 56 mp0M7zDIFggn53e2MCluP++FNgs Fig. 5E ------- COMMENT: 682113d8f12b3fe4 57 mp0M7zDIFggn53e2MCluP++FNgs Fig. 5E ------- COMMENT: 682113d8f12b3fe4 58 mp0M7zDIFggn53e2MCluP++FNgs Fig. 5E ------- COMMENT: 682113d8f12b3fe4 59 mp0M7zDIFggn53e2MCluP++FNgs Fig. 5E ------- COMMENT: 682113d8f12b3fe4 60 mp0M7zDIFggn53e2MCluP++FNgs Fig. 5E ------- COMMENT: 682113d8f12b3fe4 61 cLKKHHathQCT1JZTf0i7RHzTu8A Fig. 5F ------- COMMENT: 682113d8f12b3fe4 62 cLKKHHathQCT1JZTf0i7RHzTu8A Fig. 5F ------- COMMENT: 682113d8f12b3fe4 63 cLKKHHathQCT1JZTf0i7RHzTu8A Fig. 5F ------- COMMENT: 682113d8f12b3fe4 64 VBKX+GFn3FMuuV6uWjZAm3XuxZ0 Thus, the dual regulation of Bub1 by Bub3, suppression of ectopic activation out of the kinetochore and the promotion of its kinetochore recruitment, may play a key role in establishing the robust kinetochore-based SAC activation system. ------- COMMENT: 682113d8f12b3fe4 66 Kvhx6HnizVjoH34YOcLVmwW62ss During this analysis, we found that at least four sites (Thr 77, Thr 338, Thr 507 and Thr 552) are phosphorylated by Mph1 in vitro (Supplementary Fig. S2). ------- COMMENT: 682113d8f12b3fe4 67 Kvhx6HnizVjoH34YOcLVmwW62ss During this analysis, we found that at least four sites (Thr 77, Thr 338, Thr 507 and Thr 552) are phosphorylated by Mph1 in vitro (Supplementary Fig. S2). ------- COMMENT: 682113d8f12b3fe4 68 Kvhx6HnizVjoH34YOcLVmwW62ss During this analysis, we found that at least four sites (Thr 77, Thr 338, Thr 507 and Thr 552) are phosphorylated by Mph1 in vitro (Supplementary Fig. S2). ------- COMMENT: 682113d8f12b3fe4 69 Kvhx6HnizVjoH34YOcLVmwW62ss During this analysis, we found that at least four sites (Thr 77, Thr 338, Thr 507 and Thr 552) are phosphorylated by Mph1 in vitro (Supplementary Fig. S2). ------- COMMENT: 682113d8f12b3fe4 70 mp0M7zDIFggn53e2MCluP++FNgs Fig. 5E ------- COMMENT: 682113d8f12b3fe4 71 YGPN9PfupgLNWdVUiqNq6OnuBEg although the kinetochore localization of Spc7-12A protein was intact (Fig. 2f), ------- COMMENT: 682113d8f12b3fe4 72 KukYFdFVFiaLtg98AYVHo8FIxgs Accordingly, the ectopic localization of Bub1 and the mitotic delay induced by Cnp3C–Mph1 were abolished by the spc7-12A mutation (Supplementary Fig. S3a) ------- COMMENT: 682113d8f12b3fe4 73 gLHEZS/RUqaKaX4QPSHqLuBpelQ Fig. 2I ------- COMMENT: 682113d8f12b3fe4 74 gLHEZS/RUqaKaX4QPSHqLuBpelQ Fig. 2I ------- COMMENT: 682763c91da26aea 1 pYMeoahmb8NcwaBuSuoiREFr8is Delayed nuclear congresion in dhc1D (Fig. 1) and double deletion dhc1D klp2D completely abolishes nuclear congression (Fig. 1)) ------- COMMENT: 682763c91da26aea 2 sXNsqcvQcjwCZufYijjvYk3VdTA Delayed nuclear congresion in klp2D (Fig. 1) and double deletion dhc1D klp2D completely abolishes nuclear congression (Fig. 1)) ------- COMMENT: 682763c91da26aea 3 Ty4I3jriwifMb1SJcx3h08hJTng (comment: Phenocopies dhc1) ------- COMMENT: 682763c91da26aea 4 pygHyrFXWWG4L0HFSziYPxWak9U ------- COMMENT: 682763c91da26aea 5 pygHyrFXWWG4L0HFSziYPxWak9U ------- COMMENT: 682763c91da26aea 6 Xb1L1D0oeozHWjRnmVjhdBSDo98 Fig. S3A ------- COMMENT: 682763c91da26aea 7 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 682763c91da26aea 8 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 682763c91da26aea 9 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 682763c91da26aea 10 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 682763c91da26aea 11 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 682763c91da26aea 12 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 682763c91da26aea 13 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 682763c91da26aea 14 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 682763c91da26aea 15 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 682763c91da26aea 16 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 682763c91da26aea 17 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 682763c91da26aea 18 KXg28VcZkGVctzwWSCK9Xc66weM (comment: mild rescue of FYPO:0000513) ------- COMMENT: 682763c91da26aea 19 ahXXHvw2felGLwufB1Y7vWYro30 Single deletion slows down nuclear congression (minus-end diretcted), double deletion with klp2 inhibits it. ------- COMMENT: 682763c91da26aea 20 FoEccrt8SfOrLm0kPEA+f5egGr8 Single deletion slows down nuclear congression (minus-end diretcted), double deletion with dhc1 inhibits it. ------- COMMENT: 684efd3d317a735a 5 qJZbSpqM9vmnOu3AxPb4Pk0Htmo We tested in vitro interaction between His6-SLD2 and recombinant wild-type GST-Ubc9 or GST-Ubc9H20D by GSH-Sepharose pulldown and Western analysis, revealing that GST-Ubc9H20D abolishes the interaction (Fig. 2A). ------- COMMENT: 684efd3d317a735a 7 7A6VVZcRf8mJtr0zHhPmZGRvEbw Strikingly, compared to SUMO , SUMOD81R cells exhibited nearly wild-type growth rates and sensitivity to genotoxins (Fig. 3D). ------- COMMENT: 684efd3d317a735a 9 Grx2OirKeujWtmgC/8+s54qRGGg The positive control of wild-type SUMO forms a ladder of conjugates that correspond to the molecular weight of SUMO species ranging from di-SUMO to high-order poly-SUMO conjugates (Fig. 3C), which are absent when a nonconjugatable form of SUMO is expressed (Fig. 3C, SUMO- gg). SUMOD81R is conjugation proficient as it produced a sumoylation pattern indistinguishable from that of the wild type (Fig. 3C). ------- COMMENT: 684efd3d317a735a 10 1QBWmpqdV2f89I1TOh/hdLswoL8 We next analyzed total SUMO conjugates in SUMOD81R cells by Western blotting for comparison with those in reference strains. Notably, in both SUMOD81R and pli1  cells, the wild-type pattern of SUMO conjugates was undetectable (Fig. 3E). ------- COMMENT: 684efd3d317a735a 11 11KqJmglw9GPHolVeOCZkEdLSUI K14E at the E1 binding site, which caused HU sensitivity (Fig. 2C) ------- COMMENT: 684efd3d317a735a 12 /0C0eTqp6FmzVr5C1M8AHgIuWa8 In addition, we identified two mutations, P21L and F24S, at the noncovalent Ubc9:SLD2 interface that also result in HU sensitivity (Fig. 2C). ------- COMMENT: 684efd3d317a735a 13 /0C0eTqp6FmzVr5C1M8AHgIuWa8 In addition, we identified two mutations, P21L and F24S, at the noncovalent Ubc9:SLD2 interface that also result in HU sensitivity (Fig. 2C). ------- COMMENT: 684efd3d317a735a 14 UBD6RqgWuqwUOWPmAcPJrt9d+3g We confirmed this hypothesis through in vitro binding assays of recombinant SUMO and Ubc9/ Ubc9H20D (Fig. 3A). ------- COMMENT: 684efd3d317a735a 15 b7Vhtx8MzoAW4C/AODqaK1ZdphQ The rad60E380R mutant exhibits elevated SUMO conjugates before and after HU treatment, which, based on the foregoing results, is most likely the result of replicative stress in the sickly rad60E380R strain. Therefore ------- COMMENT: 684efd3d317a735a 16 etrsQ3SQuUWACznayjyYVd8gwj4 In contrast, bulk SUMO conjugates were readily detected in cells lacking Nse2 SUMO ligase activity (nse2-SA) or the Ubc9:SLD2 complex (Fig. 3E, rad60E380R). ------- COMMENT: 684efd3d317a735a 17 1mLjZrKnDICeO7qUKZbMFDtHe6c Thus, both Pli1 and the noncovalent Ubc9:SUMO complex are critical for bulk sumoylation but not for modifying key targets in the DNA repair response ------- COMMENT: 684efd3d317a735a 18 1mLjZrKnDICeO7qUKZbMFDtHe6c Thus, both Pli1 and the noncovalent Ubc9:SUMO complex are critical for bulk sumoylation but not for modifying key targets in the DNA repair response ------- COMMENT: 684efd3d317a735a 19 ZAqmR+zpo+861nT0rbGJjg0TvbI Thus, both Pli1 and the noncovalent Ubc9:SUMO complex are critical for bulk sumoylation but not for modifying key targets in the DNA repair response (than pmt3) ------- COMMENT: 684efd3d317a735a 21 Uw5T2MQJ4826d+QOyJZndiHpvRA Importantly, however, similar SUMO species were induced by HU in both the rad60-4 and cds1  strains in which Rad60 is both constitutively nuclear and its Ubc9 interface is intact (Fig. 3H) ------- COMMENT: 684efd3d317a735a 22 /FwDVnA6PR3habeOyWJg+XExkys Interestingly, rad60E380R mutant cells are hypersensitive to the same DNA-damaging agents as nse2-SA cells (Fig. 4A). ------- COMMENT: 684efd3d317a735a 23 /FwDVnA6PR3habeOyWJg+XExkys Interestingly, rad60E380R mutant cells are hypersensitive to the same DNA-damaging agents as nse2-SA cells (Fig. 4A). ------- COMMENT: 684efd3d317a735a 24 /FwDVnA6PR3habeOyWJg+XExkys Interestingly, rad60E380R mutant cells are hypersensitive to the same DNA-damaging agents as nse2-SA cells (Fig. 4A). ------- COMMENT: 684efd3d317a735a 25 /FwDVnA6PR3habeOyWJg+XExkys Interestingly, rad60E380R mutant cells are hypersensitive to the same DNA-damaging agents as nse2-SA cells (Fig. 4A). ------- COMMENT: 684efd3d317a735a 26 /FwDVnA6PR3habeOyWJg+XExkys Interestingly, rad60E380R mutant cells are hypersensitive to the same DNA-damaging agents as nse2-SA cells (Fig. 4A). ------- COMMENT: 684efd3d317a735a 27 /FwDVnA6PR3habeOyWJg+XExkys Interestingly, rad60E380R mutant cells are hypersensitive to the same DNA-damaging agents as nse2-SA cells (Fig. 4A). ------- COMMENT: 684efd3d317a735a 28 6GI2dldig1j+2liT1TPHkdPBDuc However, like pli1  cells, SUMOD81R mutants are not appreciably sensitive to genotoxins (Fig. 4A) ------- COMMENT: 684efd3d317a735a 29 6GI2dldig1j+2liT1TPHkdPBDuc However, like pli1  cells, SUMOD81R mutants are not appreciably sensitive to genotoxins (Fig. 4A) ------- COMMENT: 684efd3d317a735a 30 6GI2dldig1j+2liT1TPHkdPBDuc However, like pli1  cells, SUMOD81R mutants are not appreciably sensitive to genotoxins (Fig. 4A) ------- COMMENT: 684efd3d317a735a 31 6GI2dldig1j+2liT1TPHkdPBDuc However, like pli1  cells, SUMOD81R mutants are not appreciably sensitive to genotoxins (Fig. 4A) ------- COMMENT: 684efd3d317a735a 32 6GI2dldig1j+2liT1TPHkdPBDuc However, like pli1  cells, SUMOD81R mutants are not appreciably sensitive to genotoxins (Fig. 4A) ------- COMMENT: 684efd3d317a735a 33 9szqcKp8oxvaddc9ncJR5oG5mGA These data provide compelling support for the role of Ubc9:SUMO in facilitating Pli1-dependent sumoylation, which is toxic in STUbL mutant cells. ------- COMMENT: 684efd3d317a735a 34 aAcciE3liB2cbgEe8aeUGPbcTz0 Interestingly, upon STUbL inactivation SUMOD81R forms a major di-SUMO species but none of the higher-molecular- weight chains observed with wild-type SUMO (Fig. 5B). ------- COMMENT: 685768d63e59f072 1 E39iyGKUmh3DDuTcMDmkfomfuIw In the null mutant and the mutant without SNARE domain the fluorescence of prevacuolar endosome markers is reduced, and Cps1 processing is abnormal ------- COMMENT: 685768d63e59f072 2 WKRDioaVQOOkX/p8kgzc/DWA5MA co-localization with Vps35 and with Vps27 ------- COMMENT: 685768d63e59f072 3 xvOzFMb/45rI17Bs3VuBtM/NwnQ co-localization with Cfr1 ------- COMMENT: 685768d63e59f072 4 ny4HTwxoojxTnmir58XFaQZnShc ------- COMMENT: 685768d63e59f072 5 EAN5dXS2Plsl8LAJOUoydoMXyXw Interacts with a Vps29-Vps35-Vps26 fusion protein ------- COMMENT: 685768d63e59f072 6 WK8GxW37sgVjH8OxGVQPSpPakxo ------- COMMENT: 685768d63e59f072 7 uUjdQnuQDdmfmbAz/df1xKdyj1k (comment: CONDITION 80 mM MgCl2) ------- COMMENT: 685768d63e59f072 8 5S1yPNADYa/mPRnfxi3zE/uLBJ0 (comment: CONDITION 1.0 M KCl) ------- COMMENT: 685768d63e59f072 9 aWzSwtpRvt/iba9dFU33sHGUpTE (comment: Affecting Cps1 carboxypeptidase) ------- COMMENT: 685768d63e59f072 10 aWzSwtpRvt/iba9dFU33sHGUpTE (comment: Affecting Cps1 carboxypeptidase) ------- COMMENT: 685768d63e59f072 11 iMjoIVOxs4cfoeQlZoyTsxon8uw (comment: CHECK Affecting Vps10, Vps27, Vps35, Pep12 and the PI(3)P probe Cherry-FYVE) ------- COMMENT: 685768d63e59f072 14 ahnVZkNOfGcWfbZCF0f5s138Id4 (comment: CHECK Affecting Vps10 and the PI(3) probe Cherry-FYVE) ------- COMMENT: 685768d63e59f072 17 ThZ2kT8HlTB4myMyX6pTa3voou8 The mutant protein is observed at the vacuolar surface ------- COMMENT: 685768d63e59f072 18 Cs6JBeId49lKl/BxdhMWSeAS9b4 The mutant protein is observed faintly at the vacuolar surface of a low percentage of cells ------- COMMENT: 685768d63e59f072 20 w10VweHozm2zbo4Co+V/5SGT5fo (comment: same as vps35delta alone) ------- COMMENT: 685768d63e59f072 21 w10VweHozm2zbo4Co+V/5SGT5fo (comment: same as vps35delta alone) ------- COMMENT: 685768d63e59f072 23 ThZ2kT8HlTB4myMyX6pTa3voou8 The mutant protein is observed at the vacuolar surface ------- COMMENT: 685768d63e59f072 24 Cs6JBeId49lKl/BxdhMWSeAS9b4 The mutant protein is observed faintly at the vacuolar surface of a low percentage of cells ------- COMMENT: 685768d63e59f072 26 ThZ2kT8HlTB4myMyX6pTa3voou8 The mutant protein is observed at the vacuolar surface ------- COMMENT: 685768d63e59f072 27 ThZ2kT8HlTB4myMyX6pTa3voou8 The mutant protein is observed at the vacuolar surface ------- COMMENT: 685768d63e59f072 28 ThZ2kT8HlTB4myMyX6pTa3voou8 The mutant protein is observed at the vacuolar surface ------- COMMENT: 685768d63e59f072 29 ThZ2kT8HlTB4myMyX6pTa3voou8 The mutant protein is observed at the vacuolar surface ------- COMMENT: 685768d63e59f072 30 ThZ2kT8HlTB4myMyX6pTa3voou8 The mutant protein is observed at the vacuolar surface ------- COMMENT: 685768d63e59f072 31 ThZ2kT8HlTB4myMyX6pTa3voou8 The mutant protein is observed at the vacuolar surface ------- COMMENT: 685768d63e59f072 32 ThZ2kT8HlTB4myMyX6pTa3voou8 The mutant protein is observed at the vacuolar surface ------- COMMENT: 685768d63e59f072 33 Cs6JBeId49lKl/BxdhMWSeAS9b4 The mutant protein is observed faintly at the vacuolar surface of a low percentage of cells ------- COMMENT: 685768d63e59f072 34 ThZ2kT8HlTB4myMyX6pTa3voou8 The mutant protein is observed at the vacuolar surface ------- COMMENT: 685768d63e59f072 35 ThZ2kT8HlTB4myMyX6pTa3voou8 The mutant protein is observed at the vacuolar surface ------- COMMENT: 685768d63e59f072 36 ThZ2kT8HlTB4myMyX6pTa3voou8 The mutant protein is observed at the vacuolar surface ------- COMMENT: 685768d63e59f072 37 ThZ2kT8HlTB4myMyX6pTa3voou8 The mutant protein is observed at the vacuolar surface ------- COMMENT: 685768d63e59f072 38 ThZ2kT8HlTB4myMyX6pTa3voou8 The mutant protein is observed at the vacuolar surface ------- COMMENT: 685768d63e59f072 39 ThZ2kT8HlTB4myMyX6pTa3voou8 The mutant protein is observed at the vacuolar surface ------- COMMENT: 685768d63e59f072 40 ThZ2kT8HlTB4myMyX6pTa3voou8 The mutant protein is observed at the vacuolar surface ------- COMMENT: 685768d63e59f072 41 ThZ2kT8HlTB4myMyX6pTa3voou8 The mutant protein is observed at the vacuolar surface ------- COMMENT: 685768d63e59f072 42 Cs6JBeId49lKl/BxdhMWSeAS9b4 The mutant protein is observed faintly at the vacuolar surface of a low percentage of cells ------- COMMENT: 685768d63e59f072 55 SdrHQNUnvrXw2WMuFuKK0jO1KmQ (comment: CHECK same as fsv1delta alone) ------- COMMENT: 685768d63e59f072 68 aWzSwtpRvt/iba9dFU33sHGUpTE (comment: Affecting Cps1 carboxypeptidase) ------- COMMENT: 685768d63e59f072 81 aWzSwtpRvt/iba9dFU33sHGUpTE (comment: Affecting Cps1 carboxypeptidase) ------- COMMENT: 685768d63e59f072 82 aWzSwtpRvt/iba9dFU33sHGUpTE (comment: Affecting Cps1 carboxypeptidase) ------- COMMENT: 685768d63e59f072 83 aWzSwtpRvt/iba9dFU33sHGUpTE (comment: Affecting Cps1 carboxypeptidase) ------- COMMENT: 68637de9f01b5872 3 ShZkBUteoy3IxuuyMLGJoKc8qJo The loss of Dicer (Dcr1) or Argonaute (Ago1) caused only partial or no reduction in H3K9me at heterochromatin islands except island 5, which showed considerable reduction of H3K9me (fig. S4, A and B). . Moreover, de novo targeting of H3K9me to ssm4 and mei4 occurred even in the absence of Ago1, albeit at levels lower than those of the wild type (fig. S4C), suggesting that additional RNAi-independent mechanism(s) target heterochromatin to meiotic loci. W ------- COMMENT: 68637de9f01b5872 5 NOdNJfN4iyS+eG1T9X+NJquMlUE Deletion of sir2 encoding a nicotinamide adenine dinucleotide– dependent HDAC (3) caused defective H3K9me at the majority of islands (fig. S5A), but SHREC subunits were dispensable (fig. S5B). ------- COMMENT: 68637de9f01b5872 34 LQDHFjRLwz450sNNhArSIZIeLYw (comment: CHECK during vegetative growth, near genes normally expressed in meiotic cell cycle) ------- COMMENT: 68637de9f01b5872 36 LQDHFjRLwz450sNNhArSIZIeLYw (comment: CHECK during vegetative growth, near genes normally expressed in meiotic cell cycle) ------- COMMENT: 68637de9f01b5872 37 EhJ30JoNE4rKu/GXo0I7h+8l3yM (comment: CHECK negative ::) ------- COMMENT: 68637de9f01b5872 39 5lPWIMoKC0+ig6vMaBQ5CG5y97U Insertion of the mei4 DSR at the 3′ untranslated region of ura4 resulted in H3K9me at this site, especially when ura4-DSR was expressed (Fig. 2C), and ssm4 lacking its DSR failed to nucleate H3K9me (fig. S8). ------- COMMENT: 68fc7415171678cd 1 3E8BxTwukmTkrxJXgPsXQQWwnJg elg1∆ exhibits reduced direct repeat recombination associated with replication fork collapse at the RTS1 replication fork barrier ------- COMMENT: 68fc7415171678cd 2 QmeJTwFccVq9fwkV4RSk6nlphb4 Figure 3A; (comment: CHECK increased spontaneous direct repeat recombination) ------- COMMENT: 68fc7415171678cd 3 U9YWlko8hY4oUPThmrBVyrec3F0 fbh1∆ suppresses the reduced direct repeat recombination (associated with replication fork collapse at the RTS1 replication fork barrier) of an elg1∆ mutant ------- COMMENT: 68fc7415171678cd 4 H0ACNxyRrCHTH45GyWYgbmA5Nf8 pcn1(D150E) suppresses the reduced direct repeat recombination (associated with replication fork collapse at the RTS1 replication fork barrier) of an elg1∆ mutant ------- COMMENT: 68fc7415171678cd 5 ffka1qZ0X8ndHhLMjWWDAtpg6XA srs2∆ partially suppresses the reduced direct repeat recombination (associated with replication fork collapse at the RTS1 replication fork barrier) of an elg1∆ mutant ------- COMMENT: 68fc7415171678cd 7 6eoqLJLXdfz+WwxFCdUrtTiJCI4 PCNA foci persist longer than normal, and form large bright patches before disappearing (Fig 2). ------- COMMENT: 68fc7415171678cd 10 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: 68fc7415171678cd 11 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: 68fc7415171678cd 12 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 68fc7415171678cd 13 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 68fc7415171678cd 14 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 68fc7415171678cd 15 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 68fc7415171678cd 16 stvOje9clGuCl+9WPU++ikn96C0 Fig 4; (comment: very small difference from fbh1delta alone) ------- COMMENT: 68fc7415171678cd 17 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: 68fc7415171678cd 18 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: 6913a3b5e8aa3c2b 20 xBocE8c8Jvio5CewfBKtuyCIIo8 (comment: severe when both cells are cpb1delta) ------- COMMENT: 69269d206e6ff52a 1 job7MMFfwrLoiN7/qq1howAqYF0 Fig1 B ------- COMMENT: 69269d206e6ff52a 2 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 69269d206e6ff52a 3 eY1hgpChGj6Mgmp5EBp21ZlUYC4 Table 1, Figure 1B appearance of IC peak at early timepoint ------- COMMENT: 69269d206e6ff52a 4 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: 69269d206e6ff52a 5 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: 69269d206e6ff52a 6 Icclkh6MEVMn6HNOjRxa3I2OTIA Table 1, Figure 2C ------- COMMENT: 69269d206e6ff52a 7 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 69269d206e6ff52a 8 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 69269d206e6ff52a 9 CQGtsMsb1Fz3rI4URnEB3BTVeqI Data not shown ------- COMMENT: 69599d2112745bb8 1 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 69599d2112745bb8 3 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 69599d2112745bb8 4 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 69599d2112745bb8 9 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 69599d2112745bb8 13 JUKiF37j64aMaKpSH0OBgfVgnXQ Fig. 8A ------- COMMENT: 69599d2112745bb8 14 JUKiF37j64aMaKpSH0OBgfVgnXQ Fig. 8A ------- COMMENT: 69599d2112745bb8 15 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: 69599d2112745bb8 16 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: 69599d2112745bb8 17 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: 69599d2112745bb8 18 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 19 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 20 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 21 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 22 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 23 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 24 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 25 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 26 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 27 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 28 O3VYjrxPx0J5QB1JogwgkROVtt8 Fig. 10 ------- COMMENT: 69599d2112745bb8 29 O3VYjrxPx0J5QB1JogwgkROVtt8 Fig. 10 ------- COMMENT: 69599d2112745bb8 30 O3VYjrxPx0J5QB1JogwgkROVtt8 Fig. 10 ------- COMMENT: 69599d2112745bb8 31 O3VYjrxPx0J5QB1JogwgkROVtt8 Fig. 10 ------- COMMENT: 69599d2112745bb8 32 O3VYjrxPx0J5QB1JogwgkROVtt8 Fig. 10 ------- COMMENT: 69599d2112745bb8 33 O3VYjrxPx0J5QB1JogwgkROVtt8 Fig. 10 ------- COMMENT: 69599d2112745bb8 34 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 35 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 36 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 37 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 38 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 39 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 40 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 41 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 44 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 45 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 46 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 47 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 48 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 51 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 52 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 53 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 58 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 69599d2112745bb8 59 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 69599d2112745bb8 60 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 69599d2112745bb8 61 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 69599d2112745bb8 62 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 69599d2112745bb8 63 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 69599d2112745bb8 64 O3VYjrxPx0J5QB1JogwgkROVtt8 Fig. 10 ------- COMMENT: 69599d2112745bb8 65 JUKiF37j64aMaKpSH0OBgfVgnXQ Fig. 8A ------- COMMENT: 69599d2112745bb8 66 9nwBRnyCNITpNGHnDzsbOgD3FrE Fig. 5A, 6A and 7 ------- COMMENT: 69599d2112745bb8 67 WxG+BN2JY92BpK8T8RjRqaQh2xE Fig. 6B and 7 ------- COMMENT: 69599d2112745bb8 68 NCQwLPmqWo0/PvrrygKPqJcjQkc Fig. 6C and 7 ------- COMMENT: 69599d2112745bb8 69 NCQwLPmqWo0/PvrrygKPqJcjQkc Fig. 6C and 7 ------- COMMENT: 69599d2112745bb8 70 JUKiF37j64aMaKpSH0OBgfVgnXQ Fig. 8A ------- COMMENT: 69599d2112745bb8 71 JUKiF37j64aMaKpSH0OBgfVgnXQ Fig. 8A ------- COMMENT: 69599d2112745bb8 72 JUKiF37j64aMaKpSH0OBgfVgnXQ Fig. 8A ------- COMMENT: 69599d2112745bb8 73 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 74 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 75 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 76 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 77 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 78 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 79 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 80 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 81 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 82 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 83 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 84 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 85 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 86 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 87 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 88 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 69599d2112745bb8 89 O3VYjrxPx0J5QB1JogwgkROVtt8 Fig. 10 ------- COMMENT: 69599d2112745bb8 90 O3VYjrxPx0J5QB1JogwgkROVtt8 Fig. 10 ------- COMMENT: 69599d2112745bb8 91 O3VYjrxPx0J5QB1JogwgkROVtt8 Fig. 10 ------- COMMENT: 69599d2112745bb8 92 O3VYjrxPx0J5QB1JogwgkROVtt8 Fig. 10 ------- COMMENT: 69599d2112745bb8 93 O3VYjrxPx0J5QB1JogwgkROVtt8 Fig. 10 ------- COMMENT: 69599d2112745bb8 94 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 95 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 96 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 97 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 98 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 99 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 100 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69599d2112745bb8 101 74hAJeikGPU1EXRqsUT4bv58TsE Fig. 11 ------- COMMENT: 69615b5c56b7a12f 17 HoUIkqaea2q1Y7lw0+pRLWDAu58 ------- COMMENT: 697b0dd703952f05 40 b/jHXfIQMGcINVy9aQVzGAI+P40 (comment: CHECK hydroxyurea absent) ------- COMMENT: 6990ce523b5ad1e1 3 VJmmvDSSnQ7iKuFBDMC/dQhqbIc The Δght5 strain is always in 27 a "low glucose state" even when cultured in high glucose medium, and this may be the reason why the 28 lifespan extension phenotype appears. ------- COMMENT: 6990ce523b5ad1e1 14 IrEWJItlbyGLzqwIgoAUnLEhA3A (comment: CHECK decreased) ------- COMMENT: 699531ab309cc4ad 1 Zno40JIIfMde9u5cNVEbdVzh+iw Fig. 1B and C ------- COMMENT: 699531ab309cc4ad 2 Zno40JIIfMde9u5cNVEbdVzh+iw Fig. 1B and C ------- COMMENT: 699531ab309cc4ad 3 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 699531ab309cc4ad 4 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 699531ab309cc4ad 5 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 699531ab309cc4ad 6 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 699531ab309cc4ad 9 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 699531ab309cc4ad 10 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 699531ab309cc4ad 12 MpML2E+8wq5nwC1YfM2LDELzzPU Fig. 2E ------- COMMENT: 699531ab309cc4ad 13 fogVowcObkoWi79lNpy/RoRGREw Fig. 2G ------- COMMENT: 699531ab309cc4ad 14 t8sxkEPL2zX0/qNJk9Y6MIWvfzQ Chp2’s function is not exclusively associated with the establishment step and that its continued activity is critical for the maintenance of heterochromatin. Fig. 3 ------- COMMENT: 699531ab309cc4ad 15 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 699531ab309cc4ad 16 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 699531ab309cc4ad 17 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 699531ab309cc4ad 18 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 699531ab309cc4ad 19 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 699531ab309cc4ad 20 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 699531ab309cc4ad 21 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 699531ab309cc4ad 22 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 699531ab309cc4ad 23 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 699531ab309cc4ad 24 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 699531ab309cc4ad 25 vkT8uMpiBxgGlmYXEA8V3+LXigM Fig. 4E ------- COMMENT: 699531ab309cc4ad 26 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 699531ab309cc4ad 27 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 699531ab309cc4ad 28 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 699531ab309cc4ad 29 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 699531ab309cc4ad 30 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 699531ab309cc4ad 31 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 699531ab309cc4ad 32 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 699531ab309cc4ad 33 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 699531ab309cc4ad 34 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 699531ab309cc4ad 35 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 699531ab309cc4ad 36 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 699531ab309cc4ad 37 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 699531ab309cc4ad 38 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 699531ab309cc4ad 39 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 699531ab309cc4ad 40 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 699531ab309cc4ad 41 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 699531ab309cc4ad 42 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 699531ab309cc4ad 43 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 699531ab309cc4ad 44 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 699531ab309cc4ad 45 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 699531ab309cc4ad 46 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 699531ab309cc4ad 47 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 699531ab309cc4ad 48 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 699531ab309cc4ad 49 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 699531ab309cc4ad 50 sZ6VeYXOlL471tjkcyQrhCJ5I8k Fig. 8D ------- COMMENT: 699531ab309cc4ad 51 sZ6VeYXOlL471tjkcyQrhCJ5I8k Fig. 8D ------- COMMENT: 699531ab309cc4ad 52 sZ6VeYXOlL471tjkcyQrhCJ5I8k Fig. 8D ------- COMMENT: 699531ab309cc4ad 53 sZ6VeYXOlL471tjkcyQrhCJ5I8k Fig. 8D ------- COMMENT: 699531ab309cc4ad 54 Fk+vS+dhZzQTd5v1DDSy6Une3Ls Fig. 8H ------- COMMENT: 699531ab309cc4ad 55 Fk+vS+dhZzQTd5v1DDSy6Une3Ls Fig. 8H ------- COMMENT: 699531ab309cc4ad 56 Fk+vS+dhZzQTd5v1DDSy6Une3Ls Fig. 8H ------- COMMENT: 699531ab309cc4ad 57 Fk+vS+dhZzQTd5v1DDSy6Une3Ls Fig. 8H ------- COMMENT: 699531ab309cc4ad 58 FkWjCHPXelPQLtr1AkZdsc+Y128 Fig. 9A ------- COMMENT: 699531ab309cc4ad 59 FkWjCHPXelPQLtr1AkZdsc+Y128 Fig. 9A ------- COMMENT: 699531ab309cc4ad 60 rPid98WfnsfSqrHo+yYiNamohYs Fig. 9C ------- COMMENT: 699531ab309cc4ad 61 MnEx64B/zE1VdKIuzdt/kZIfiSk Fig. 9D ------- COMMENT: 699531ab309cc4ad 62 MnEx64B/zE1VdKIuzdt/kZIfiSk Fig. 9D ------- COMMENT: 699def6a055fb333 1 zUFkH+nVBQKBIqUvvJiBVMbXSCo (comment: CHECK 200 Gy; Andres SN et al. (2019)) ------- COMMENT: 699def6a055fb333 2 N3n7Bl04z/JSjdweTGscX6fILzc (comment: CHECK 75 J/m^2; Andres SN et al. (2019)) ------- COMMENT: 699def6a055fb333 3 Y3rnL26iEmW1+vf6snkGYCp41l0 (comment: CHECK Andres SN et al. (2019)) ------- COMMENT: 69a310e75d21c964 1 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 2 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 3 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 4 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 5 AanVMGTQfzGgBv8Vpfjvs/79bxY (Table S1, Fig. S2A) ------- COMMENT: 69a310e75d21c964 6 5rWxS9fXwQZIjBJ8A4gii+uKZbg (Table S1, Fig. S3A) ------- COMMENT: 69a310e75d21c964 7 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 8 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 9 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 10 5rWxS9fXwQZIjBJ8A4gii+uKZbg (Table S1, Fig. S3A) ------- COMMENT: 69a310e75d21c964 11 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 12 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 13 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 14 5rWxS9fXwQZIjBJ8A4gii+uKZbg (Table S1, Fig. S3A) ------- COMMENT: 69a310e75d21c964 15 5rWxS9fXwQZIjBJ8A4gii+uKZbg (Table S1, Fig. S3A) ------- COMMENT: 69a310e75d21c964 16 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 17 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 18 5rWxS9fXwQZIjBJ8A4gii+uKZbg (Table S1, Fig. S3A) ------- COMMENT: 69a310e75d21c964 19 5rWxS9fXwQZIjBJ8A4gii+uKZbg (Table S1, Fig. S3A) ------- COMMENT: 69a310e75d21c964 20 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 21 5rWxS9fXwQZIjBJ8A4gii+uKZbg (Table S1, Fig. S3A) ------- COMMENT: 69a310e75d21c964 22 5rWxS9fXwQZIjBJ8A4gii+uKZbg (Table S1, Fig. S3A) ------- COMMENT: 69a310e75d21c964 23 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 24 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 25 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 26 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 27 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 28 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 29 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 30 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 31 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 32 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 33 AanVMGTQfzGgBv8Vpfjvs/79bxY (Table S1, Fig. S2A) ------- COMMENT: 69a310e75d21c964 34 AanVMGTQfzGgBv8Vpfjvs/79bxY (Table S1, Fig. S2A) ------- COMMENT: 69a310e75d21c964 35 AanVMGTQfzGgBv8Vpfjvs/79bxY (Table S1, Fig. S2A) ------- COMMENT: 69a310e75d21c964 36 mNr9XOGGIQCbMg5oKk2q2eRtObQ (Table S1, Fig. 2B) ------- COMMENT: 69a310e75d21c964 37 mNr9XOGGIQCbMg5oKk2q2eRtObQ (Table S1, Fig. 2B) ------- COMMENT: 69a310e75d21c964 38 mNr9XOGGIQCbMg5oKk2q2eRtObQ (Table S1, Fig. 2B) ------- COMMENT: 69a310e75d21c964 39 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 40 5rWxS9fXwQZIjBJ8A4gii+uKZbg (Table S1, Fig. S3A) ------- COMMENT: 69a310e75d21c964 41 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 42 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 43 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 44 5rWxS9fXwQZIjBJ8A4gii+uKZbg (Table S1, Fig. S3A) ------- COMMENT: 69a310e75d21c964 45 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 46 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 47 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 48 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 49 5rWxS9fXwQZIjBJ8A4gii+uKZbg (Table S1, Fig. S3A) ------- COMMENT: 69a310e75d21c964 50 AanVMGTQfzGgBv8Vpfjvs/79bxY (Table S1, Fig. S2A) ------- COMMENT: 69a310e75d21c964 51 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 52 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 53 5rWxS9fXwQZIjBJ8A4gii+uKZbg (Table S1, Fig. S3A) ------- COMMENT: 69a310e75d21c964 54 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 55 5rWxS9fXwQZIjBJ8A4gii+uKZbg (Table S1, Fig. S3A) ------- COMMENT: 69a310e75d21c964 56 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 57 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 58 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 59 5rWxS9fXwQZIjBJ8A4gii+uKZbg (Table S1, Fig. S3A) ------- COMMENT: 69a310e75d21c964 60 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 61 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 62 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 63 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 64 5rWxS9fXwQZIjBJ8A4gii+uKZbg (Table S1, Fig. S3A) ------- COMMENT: 69a310e75d21c964 65 5rWxS9fXwQZIjBJ8A4gii+uKZbg (Table S1, Fig. S3A) ------- COMMENT: 69a310e75d21c964 66 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 67 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 68 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 69 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 70 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 71 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 72 5rWxS9fXwQZIjBJ8A4gii+uKZbg (Table S1, Fig. S3A) ------- COMMENT: 69a310e75d21c964 73 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 74 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 75 5rWxS9fXwQZIjBJ8A4gii+uKZbg (Table S1, Fig. S3A) ------- COMMENT: 69a310e75d21c964 76 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 77 AanVMGTQfzGgBv8Vpfjvs/79bxY (Table S1, Fig. S2A) ------- COMMENT: 69a310e75d21c964 78 mNr9XOGGIQCbMg5oKk2q2eRtObQ (Table S1, Fig. 2B) ------- COMMENT: 69a310e75d21c964 79 WZiiSIwIeVgxkF7Wk7WESRDkyzs (Table S1, Fig. 3A) ------- COMMENT: 69a310e75d21c964 80 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 69a310e75d21c964 81 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 69a310e75d21c964 82 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 69a310e75d21c964 83 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 69a310e75d21c964 84 gA4P48uKuk6ti5ukTRuiFvXo/Zw (Fig. S2C) ------- COMMENT: 69a310e75d21c964 85 mNr9XOGGIQCbMg5oKk2q2eRtObQ (Table S1, Fig. 2B) ------- COMMENT: 69a310e75d21c964 86 mNr9XOGGIQCbMg5oKk2q2eRtObQ (Table S1, Fig. 2B) ------- COMMENT: 69a310e75d21c964 87 WZiiSIwIeVgxkF7Wk7WESRDkyzs (Table S1, Fig. 3A) ------- COMMENT: 69a310e75d21c964 88 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 69a310e75d21c964 89 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 69a310e75d21c964 90 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 69a310e75d21c964 91 mNr9XOGGIQCbMg5oKk2q2eRtObQ (Table S1, Fig. 2B) ------- COMMENT: 69a310e75d21c964 92 WZiiSIwIeVgxkF7Wk7WESRDkyzs (Table S1, Fig. 3A) ------- COMMENT: 69a310e75d21c964 93 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 69a310e75d21c964 94 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 69a310e75d21c964 95 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 69a310e75d21c964 96 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 69a310e75d21c964 97 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: 69a310e75d21c964 98 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 69a310e75d21c964 99 tpAeqVEHQD2TwP0s+PdcBgMhHOo (Table S1) ------- COMMENT: 69a310e75d21c964 100 mNr9XOGGIQCbMg5oKk2q2eRtObQ (Table S1, Fig. 2B) ------- COMMENT: 69a310e75d21c964 101 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 69a310e75d21c964 102 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: 69a310e75d21c964 103 pQ2QvDRuUXQ4tFEUyuQUbr4kYe4 (Fig. 1E and F) ------- COMMENT: 69a310e75d21c964 104 mNr9XOGGIQCbMg5oKk2q2eRtObQ (Table S1, Fig. 2B) ------- COMMENT: 69a310e75d21c964 105 mNr9XOGGIQCbMg5oKk2q2eRtObQ (Table S1, Fig. 2B) ------- COMMENT: 69a310e75d21c964 106 mNr9XOGGIQCbMg5oKk2q2eRtObQ (Table S1, Fig. 2B) ------- COMMENT: 69a310e75d21c964 107 mNr9XOGGIQCbMg5oKk2q2eRtObQ (Table S1, Fig. 2B) ------- COMMENT: 69a310e75d21c964 108 mNr9XOGGIQCbMg5oKk2q2eRtObQ (Table S1, Fig. 2B) ------- COMMENT: 69a310e75d21c964 109 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: 69a310e75d21c964 110 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: 69a310e75d21c964 111 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: 69a310e75d21c964 112 DnQNbBCyjr9h1gZ1Ef6ybZH1kkk (Fig. 3F) ------- COMMENT: 69a310e75d21c964 113 DnQNbBCyjr9h1gZ1Ef6ybZH1kkk (Fig. 3F) ------- COMMENT: 69a310e75d21c964 114 WD3kRd80SyyYyGSzDepPv0TldzQ (Fig. 3G) ------- COMMENT: 69a310e75d21c964 115 DES5Jkiy+rZ7wehRYgobQ94L1Q8 (Fig. 2G) ------- COMMENT: 69a310e75d21c964 116 dJB6QAubOSayNATA9sbJUiUH0oE (Fig. 3H) ------- COMMENT: 69a310e75d21c964 117 dJB6QAubOSayNATA9sbJUiUH0oE (Fig. 3H) ------- COMMENT: 69a310e75d21c964 118 WZiiSIwIeVgxkF7Wk7WESRDkyzs (Table S1, Fig. 3A) ------- COMMENT: 69a310e75d21c964 119 WZiiSIwIeVgxkF7Wk7WESRDkyzs (Table S1, Fig. 3A) ------- COMMENT: 69a310e75d21c964 120 WZiiSIwIeVgxkF7Wk7WESRDkyzs (Table S1, Fig. 3A) ------- COMMENT: 69a310e75d21c964 121 WZiiSIwIeVgxkF7Wk7WESRDkyzs (Table S1, Fig. 3A) ------- COMMENT: 69a310e75d21c964 122 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 69a310e75d21c964 123 gyiLWCn6NxJajHiU/kX8pY66mI4 (Fig. 3C and D) ------- COMMENT: 69a310e75d21c964 124 gyiLWCn6NxJajHiU/kX8pY66mI4 (Fig. 3C and D) ------- COMMENT: 69a310e75d21c964 125 gyiLWCn6NxJajHiU/kX8pY66mI4 (Fig. 3C and D) ------- COMMENT: 69a310e75d21c964 126 gyiLWCn6NxJajHiU/kX8pY66mI4 (Fig. 3C and D) ------- COMMENT: 69a310e75d21c964 127 gyiLWCn6NxJajHiU/kX8pY66mI4 (Fig. 3C and D) ------- COMMENT: 69a310e75d21c964 128 gyiLWCn6NxJajHiU/kX8pY66mI4 (Fig. 3C and D) ------- COMMENT: 69a310e75d21c964 129 gyiLWCn6NxJajHiU/kX8pY66mI4 (Fig. 3C and D) ------- COMMENT: 69a310e75d21c964 130 gyiLWCn6NxJajHiU/kX8pY66mI4 (Fig. 3C and D) ------- COMMENT: 69a310e75d21c964 131 d8xLp/dA0M0C7hDu7v7MWXmIJX4 (Fig. 4C and D) ------- COMMENT: 69a310e75d21c964 132 d8xLp/dA0M0C7hDu7v7MWXmIJX4 (Fig. 4C and D) ------- COMMENT: 69a310e75d21c964 133 d8xLp/dA0M0C7hDu7v7MWXmIJX4 (Fig. 4C and D) ------- COMMENT: 69a310e75d21c964 134 4UpsW1a43iD+sQJC1myu8XcGLj4 (Fig. 5A and B) ------- COMMENT: 69a310e75d21c964 135 4UpsW1a43iD+sQJC1myu8XcGLj4 (Fig. 5A and B) ------- COMMENT: 69a310e75d21c964 136 4UpsW1a43iD+sQJC1myu8XcGLj4 (Fig. 5A and B) ------- COMMENT: 69a310e75d21c964 137 4UpsW1a43iD+sQJC1myu8XcGLj4 (Fig. 5A and B) ------- COMMENT: 69a310e75d21c964 138 4UpsW1a43iD+sQJC1myu8XcGLj4 (Fig. 5A and B) ------- COMMENT: 69a310e75d21c964 139 4UpsW1a43iD+sQJC1myu8XcGLj4 (Fig. 5A and B) ------- COMMENT: 69a310e75d21c964 140 4UpsW1a43iD+sQJC1myu8XcGLj4 (Fig. 5A and B) ------- COMMENT: 69a310e75d21c964 141 4UpsW1a43iD+sQJC1myu8XcGLj4 (Fig. 5A and B) ------- COMMENT: 69a310e75d21c964 142 4UpsW1a43iD+sQJC1myu8XcGLj4 (Fig. 5A and B) ------- COMMENT: 69a310e75d21c964 143 4UpsW1a43iD+sQJC1myu8XcGLj4 (Fig. 5A and B) ------- COMMENT: 69a310e75d21c964 144 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: 69a310e75d21c964 145 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: 69a310e75d21c964 146 tRyX+wj2TuU1VMgruorVbvut50Q (Fig. 6A and Fig. S6B) ------- COMMENT: 69a310e75d21c964 147 tRyX+wj2TuU1VMgruorVbvut50Q (Fig. 6A and Fig. S6B) ------- COMMENT: 69a310e75d21c964 148 tRyX+wj2TuU1VMgruorVbvut50Q (Fig. 6A and Fig. S6B) ------- COMMENT: 69a310e75d21c964 150 wnx2gS3IAKqzPkStlyJ0uF9GveM (Fig. 6C) ------- COMMENT: 69a310e75d21c964 151 wnx2gS3IAKqzPkStlyJ0uF9GveM (Fig. 6C) ------- COMMENT: 69a310e75d21c964 152 XwjFKIBpFnAfCL+3uv8ZHt+X4Cc (Fig. 6D) ------- COMMENT: 69a310e75d21c964 153 kIhmBd7G1n6/01SpGv20pzgvebY (Fig. 6G) ------- COMMENT: 69a310e75d21c964 154 kIhmBd7G1n6/01SpGv20pzgvebY (Fig. 6G) ------- COMMENT: 69a310e75d21c964 155 kIhmBd7G1n6/01SpGv20pzgvebY (Fig. 6G) ------- COMMENT: 69a310e75d21c964 156 kIhmBd7G1n6/01SpGv20pzgvebY (Fig. 6G) ------- COMMENT: 69a310e75d21c964 157 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: 69a310e75d21c964 158 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: 69a310e75d21c964 159 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: 69a310e75d21c964 160 xqjTztL6qGqaPgp6WgcG+iOomGI (Fig. S1B) ------- COMMENT: 69a310e75d21c964 161 xqjTztL6qGqaPgp6WgcG+iOomGI (Fig. S1B) ------- COMMENT: 69a310e75d21c964 162 UecLDdbzEF2Fd34z5as/4/0I/eg (Fig. S1E) ------- COMMENT: 69a310e75d21c964 163 UecLDdbzEF2Fd34z5as/4/0I/eg (Fig. S1E) ------- COMMENT: 69a310e75d21c964 164 UecLDdbzEF2Fd34z5as/4/0I/eg (Fig. S1E) ------- COMMENT: 69a310e75d21c964 165 UecLDdbzEF2Fd34z5as/4/0I/eg (Fig. S1E) ------- COMMENT: 69a310e75d21c964 166 UecLDdbzEF2Fd34z5as/4/0I/eg (Fig. S1E) ------- COMMENT: 69a310e75d21c964 167 UecLDdbzEF2Fd34z5as/4/0I/eg (Fig. S1E) ------- COMMENT: 69a310e75d21c964 168 UecLDdbzEF2Fd34z5as/4/0I/eg (Fig. S1E) ------- COMMENT: 69a310e75d21c964 169 AanVMGTQfzGgBv8Vpfjvs/79bxY (Table S1, Fig. S2A) ------- COMMENT: 69a310e75d21c964 170 AanVMGTQfzGgBv8Vpfjvs/79bxY (Table S1, Fig. S2A) ------- COMMENT: 69a310e75d21c964 171 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 69a310e75d21c964 172 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 69a310e75d21c964 173 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 69a310e75d21c964 174 gA4P48uKuk6ti5ukTRuiFvXo/Zw (Fig. S2C) ------- COMMENT: 69a310e75d21c964 175 gA4P48uKuk6ti5ukTRuiFvXo/Zw (Fig. S2C) ------- COMMENT: 69a310e75d21c964 176 ojGoCV7tkFuQq8EQLxws0hw4rK4 (Fig. S2D) ------- COMMENT: 69a310e75d21c964 177 ojGoCV7tkFuQq8EQLxws0hw4rK4 (Fig. S2D) ------- COMMENT: 69a310e75d21c964 178 ojGoCV7tkFuQq8EQLxws0hw4rK4 (Fig. S2D) ------- COMMENT: 69a310e75d21c964 179 ojGoCV7tkFuQq8EQLxws0hw4rK4 (Fig. S2D) ------- COMMENT: 69a310e75d21c964 180 ojGoCV7tkFuQq8EQLxws0hw4rK4 (Fig. S2D) ------- COMMENT: 69a310e75d21c964 181 tRyX+wj2TuU1VMgruorVbvut50Q (Fig. 6A and Fig. S6B) ------- COMMENT: 69a310e75d21c964 182 yYhbbbzHPoozz1GP5TKbGIhmwAY (Fig. S7C) ------- COMMENT: 69a310e75d21c964 183 68Bz+9ouR8mrmbI1EBKM8SNdm0A Our analyses show that RIXC bound to the H3K9me-Swi6 platform tethers heterochromatic regions to Amo1 at the nuclear periphery, which in turn stabilizes heterochromatin through suppression of histone turnover (Figure 7D). ------- COMMENT: 69a310e75d21c964 184 68Bz+9ouR8mrmbI1EBKM8SNdm0A Our analyses show that RIXC bound to the H3K9me-Swi6 platform tethers heterochromatic regions to Amo1 at the nuclear periphery, which in turn stabilizes heterochromatin through suppression of histone turnover (Figure 7D). ------- COMMENT: 69d5c50d65bdb7fe 1 5DWqo03puFeQtodWqcCbmJ6nfyM fig 1A ------- COMMENT: 69d5c50d65bdb7fe 2 5DWqo03puFeQtodWqcCbmJ6nfyM fig 1A ------- COMMENT: 69d5c50d65bdb7fe 3 r/Bqs8BNBRV8GYvXKzl4HFaFkAM (growing) fig 1A ------- COMMENT: 69d5c50d65bdb7fe 4 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 69d5c50d65bdb7fe 5 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 69d5c50d65bdb7fe 6 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 69d5c50d65bdb7fe 7 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 69d5c50d65bdb7fe 8 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 69d5c50d65bdb7fe 9 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 69d5c50d65bdb7fe 10 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: 69d5c50d65bdb7fe 11 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: 69d5c50d65bdb7fe 12 r4BBcVJt+i01z9OtZMxna39xhLA Figure 2D Figure 2F ------- COMMENT: 69d5c50d65bdb7fe 13 r4BBcVJt+i01z9OtZMxna39xhLA Figure 2D Figure 2F ------- COMMENT: 69d5c50d65bdb7fe 14 NTTAvTlOq0ss5aYWW9zMbCEXgzI Supplemental Figure S1, C–E ------- COMMENT: 69d5c50d65bdb7fe 15 NTTAvTlOq0ss5aYWW9zMbCEXgzI Supplemental Figure S1, C–E ------- COMMENT: 69d5c50d65bdb7fe 16 NTTAvTlOq0ss5aYWW9zMbCEXgzI Supplemental Figure S1, C–E ------- COMMENT: 69d5c50d65bdb7fe 17 5AGXFLZhbJRoknFicSMFZS1RjZo Figure 3, Supplemental Table S2, and Supplemental Figure S4 ------- COMMENT: 6a1cd0660cdba7fd 1 XGjwaJjAHMJAN+uhskmBFqZcLJg Fig1 F, H cells were pre NETO after temperature block ------- COMMENT: 6a1cd0660cdba7fd 2 gOGhTPFn7TSZjVkKWzpJpchsDHU Fig2A cdc25-22 arrest released cells ie post NETO do not branch ------- COMMENT: 6a1cd0660cdba7fd 3 uPs431BKSReZyxHjr0AjrrfAq38 Fig2 B cells were pre NETO after temperature block ------- COMMENT: 6a1cd0660cdba7fd 4 cmUPgdkAnh0qvg2eptEA9HtPhvA Fig2B cells were pre NETO after temperature block ------- COMMENT: 6a1cd0660cdba7fd 5 cmUPgdkAnh0qvg2eptEA9HtPhvA Fig2B cells were pre NETO after temperature block ------- COMMENT: 6a1cd0660cdba7fd 6 aZa4SGhtMBX5fXt/t5BXQwP4sHo Fig2C cells were pre NETO after temperature block about 5% cells are already branched at release ------- COMMENT: 6a1cd0660cdba7fd 7 aZa4SGhtMBX5fXt/t5BXQwP4sHo Fig2C cells were pre NETO after temperature block about 5% cells are already branched at release ------- COMMENT: 6a1cd0660cdba7fd 8 MU06scWYzwZcmUoTTOhgx4NAukA Fig2C arrest released cells are pre NETO but only branch at low level. ------- COMMENT: 6a1cd0660cdba7fd 9 CcFdn+190kmiYuFfLaAQqZtVUu4 Fig2C arrest released cells have NETO defect and do not branch. ------- COMMENT: 6a1cd0660cdba7fd 10 yDU+EY8Kh9KbWxrv95qAF2dSNzQ Fig 3A-C pre NETO blocked cells do not branch if TBZ is added at shift down ------- COMMENT: 6a1cd0660cdba7fd 12 3+qs2PKFPBZT4OAN4z+pClNjzBw Fig 3C cell length does not affect branching showing its not because cells are longer at high temp ------- COMMENT: 6a1cd0660cdba7fd 13 ppZ9jCokAIqKOq1eVujfZJP2MyI Fig 4 Short interphase microtubules located in the cell centre ------- COMMENT: 6a1cd0660cdba7fd 14 bHUhboU8lerbelzX5vMuJoXfsK0 Fig5 C,D ------- COMMENT: 6a1cd0660cdba7fd 15 C8vP0bSXM58h6zu40etEO+UaRvY Fig 6 F-actin localised to branch site in presence of TBZ ------- COMMENT: 6a1cd0660cdba7fd 16 MrCDIuoWcC2X0TgvUSrw4WKoO1Y Fig 6 abnormal septum in branched cell ------- COMMENT: 6a1cd0660cdba7fd 17 lEd+B6WUPoRA7HBffKvccnx695Q Fig 8A-D Actin relocalisation to old or new cell end after microtubule disruption ------- COMMENT: 6a1cd0660cdba7fd 18 qZ+3VoHEWHmuYw2/bNopDkEU47A Fig 9 tea1 can relocalise to cell ends in absence of microtubules ------- COMMENT: 6a1cd0660cdba7fd 21 4ntFhimQOQg796SB6nwcbRfhGiE Fig 11 absence of microtubules ------- COMMENT: 6a1cd0660cdba7fd 22 9KQLPqumDGnCcJmPtIqHjcdJrDA Fig 11 in the absence of microtubules and actin ------- COMMENT: 6a1cd0660cdba7fd 23 Y6xelROg336aNJZFdsVDKddhD44 Fig5 C,D Fig 12 Normal protein localisation in presence of TBZ ------- COMMENT: 6a42f3bfbbc08463 9 BNsQA5IYfHIx6/84kDia07cI7FY ------- COMMENT: 6a42f3bfbbc08463 12 TMbyaS5M07Q6rbdRioWUOecOh/E (comment: CHECK pmt3-D81R pmt3-KallR) ------- COMMENT: 6a42f3bfbbc08463 25 Ez2q9dw8CCJg1jZqs7dmDpVJCFM also assayed using ChIP to detect Rts1 binding to Npp106 ------- COMMENT: 6a42f3bfbbc08463 26 Ez2q9dw8CCJg1jZqs7dmDpVJCFM also assayed using ChIP to detect Rts1 binding to Npp106 ------- COMMENT: 6a42f3bfbbc08463 27 Ez2q9dw8CCJg1jZqs7dmDpVJCFM also assayed using ChIP to detect Rts1 binding to Npp106 ------- COMMENT: 6a42f3bfbbc08463 28 Ez2q9dw8CCJg1jZqs7dmDpVJCFM also assayed using ChIP to detect Rts1 binding to Npp106 ------- COMMENT: 6a42f3bfbbc08463 29 Ez2q9dw8CCJg1jZqs7dmDpVJCFM also assayed using ChIP to detect Rts1 binding to Npp106 ------- COMMENT: 6a42f3bfbbc08463 30 Ez2q9dw8CCJg1jZqs7dmDpVJCFM also assayed using ChIP to detect Rts1 binding to Npp106 ------- COMMENT: 6a42f3bfbbc08463 31 Ez2q9dw8CCJg1jZqs7dmDpVJCFM also assayed using ChIP to detect Rts1 binding to Npp106 ------- COMMENT: 6a42f3bfbbc08463 36 Ez2q9dw8CCJg1jZqs7dmDpVJCFM also assayed using ChIP to detect Rts1 binding to Npp106 ------- COMMENT: 6a42f3bfbbc08463 37 Ez2q9dw8CCJg1jZqs7dmDpVJCFM also assayed using ChIP to detect Rts1 binding to Npp106 ------- COMMENT: 6a42f3bfbbc08463 38 Ez2q9dw8CCJg1jZqs7dmDpVJCFM also assayed using ChIP to detect Rts1 binding to Npp106 ------- COMMENT: 6a42f3bfbbc08463 39 Ez2q9dw8CCJg1jZqs7dmDpVJCFM also assayed using ChIP to detect Rts1 binding to Npp106 ------- COMMENT: 6a42f3bfbbc08463 40 Ez2q9dw8CCJg1jZqs7dmDpVJCFM also assayed using ChIP to detect Rts1 binding to Npp106 ------- COMMENT: 6a42f3bfbbc08463 41 Ez2q9dw8CCJg1jZqs7dmDpVJCFM also assayed using ChIP to detect Rts1 binding to Npp106 ------- COMMENT: 6a42f3bfbbc08463 42 Ez2q9dw8CCJg1jZqs7dmDpVJCFM also assayed using ChIP to detect Rts1 binding to Npp106 ------- COMMENT: 6a42f3bfbbc08463 76 51jKPDIJo9CSsE9dHn0ya9Z7wS8 (comment: same as nup132delta alone) ------- COMMENT: 6a65148752d79293 25 vif5q7idlX0Yr5H6I6pfgrBe0dk (comment: global translation, not a specific gene) ------- COMMENT: 6ad90d4a01aee5f2 33 RXpBY3GTzm4Oq/uqcS4Xm/2RMZg (comment: CHECK weak sensitivity) ------- COMMENT: 6ad90d4a01aee5f2 40 +JQMdEXaNveYxxeaY9eVBDDRGHU (comment: CHECK weak sensitivity) ------- COMMENT: 6ad90d4a01aee5f2 42 zBjSy1B+o3gb/0Uh8Hp0HRe45VQ (comment: CHECK weak sensitivity) ------- COMMENT: 6ad90d4a01aee5f2 51 zBjSy1B+o3gb/0Uh8Hp0HRe45VQ (comment: CHECK weak sensitivity) ------- COMMENT: 6ad90d4a01aee5f2 53 zBjSy1B+o3gb/0Uh8Hp0HRe45VQ (comment: CHECK weak sensitivity) ------- COMMENT: 6ad90d4a01aee5f2 92 lPbYnB0FJsfu3fLqwVYMixaghxo Fluorescence microscopy of Sec13 tagged with GFP at either its N-terminal or C-terminal end. ------- COMMENT: 6ad90d4a01aee5f2 126 ANN8UD9gTeUKD/UesYVVr+WTaLs Our strains expressing GFP-tagged nucleoporins were all viable, but four of them (spNup45-GFP, spNup184-GFP, GFP-spRae1, and spNup189n-GFP) showed growth deficiencies ------- COMMENT: 6b5237fc76de962d 1 JANVttwVMFb1QEho8OMMOBh0J+s Fig. 1C, ID and 1E ------- COMMENT: 6b5237fc76de962d 2 22ILwwyMXQ3WWRL39HlsB9u2FyI Fig. 2A and 2C ------- COMMENT: 6b5237fc76de962d 3 Y2tOLrRz2aPkfaIxt6AtgxM7Axw Fig. 2A and 2B ------- COMMENT: 6b5237fc76de962d 4 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 6b5237fc76de962d 5 WHamu+TOFgRmtB4d1AIIoO2GVHg Fig. S1D and S1E ------- COMMENT: 6b5237fc76de962d 6 OeflygocAh7jqV2ZgbL9wYINk3g Supplementary Fig. S1D and S1E ------- COMMENT: 6b5237fc76de962d 7 MlOgK2hb8xk52CAVvkKN/20Pzqg Fig. 1C, Fig. 1D and Fig. 1E ------- COMMENT: 6b5237fc76de962d 8 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6b5237fc76de962d 9 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 6b5237fc76de962d 10 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 6b5237fc76de962d 11 xEygp+2JXk6u2PdKSFI9mAByzTw Fig. 1C, 1D and 1E ------- COMMENT: 6b5237fc76de962d 12 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6b5237fc76de962d 13 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 6b5237fc76de962d 14 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 6b5237fc76de962d 15 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 6b5237fc76de962d 19 YjnlKz5i1kuX95Dr5bmgYigJWTk Fig. 1A, 1B, S1A ------- COMMENT: 6b5237fc76de962d 20 YjnlKz5i1kuX95Dr5bmgYigJWTk Fig. 1A, 1B, S1A ------- COMMENT: 6b5237fc76de962d 21 YjnlKz5i1kuX95Dr5bmgYigJWTk Fig. 1A, 1B, S1A ------- COMMENT: 6b5237fc76de962d 22 22ILwwyMXQ3WWRL39HlsB9u2FyI Fig. 2A and 2C ------- COMMENT: 6b5237fc76de962d 23 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 6b5237fc76de962d 24 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 6b5ad24ed09ee3d8 1 coGbbyw23fJc83Yp2KWG4dP5Dzo Figure 1b (comment: live cell observation) ------- COMMENT: 6b5ad24ed09ee3d8 2 coGbbyw23fJc83Yp2KWG4dP5Dzo Figure 1b (comment: live cell observation) ------- COMMENT: 6b5ad24ed09ee3d8 3 coGbbyw23fJc83Yp2KWG4dP5Dzo Figure 1b (comment: live cell observation) ------- COMMENT: 6b5ad24ed09ee3d8 4 zrySppqAVs6XYFpI38b3+99WLFM Figure 1b (comment: live cell observation) ------- COMMENT: 6b5ad24ed09ee3d8 5 9e/i15obwhuc0VJZbRK49rGjgSA Figure 1b, Figure 4b (comment: live cell observation) ------- COMMENT: 6b5ad24ed09ee3d8 6 p+oVe0jJEd3iUOZ6+ONJVCdjeM4 Figure 4A ------- COMMENT: 6b5ad24ed09ee3d8 7 coGbbyw23fJc83Yp2KWG4dP5Dzo Figure 1b (comment: live cell observation) ------- COMMENT: 6b5ad24ed09ee3d8 8 coGbbyw23fJc83Yp2KWG4dP5Dzo Figure 1b (comment: live cell observation) ------- COMMENT: 6b5ad24ed09ee3d8 9 coGbbyw23fJc83Yp2KWG4dP5Dzo Figure 1b (comment: live cell observation) ------- COMMENT: 6b5ad24ed09ee3d8 10 9JRh7WJXtKmhOWnywlFEh0KHMv4 Figure 1b (comment: live cell observation) ------- COMMENT: 6b5ad24ed09ee3d8 11 W+qTVppcIahw3J28hKW92H/7Pwg Figure 4a. (comment: homologous pairing examined at C24 locus) ------- COMMENT: 6b5ad24ed09ee3d8 23 Ocx+iB/95EBAbk5q7f3QrSYRqPw Figure 1c ------- COMMENT: 6b5ad24ed09ee3d8 24 Ocx+iB/95EBAbk5q7f3QrSYRqPw Figure 1c ------- COMMENT: 6b5ad24ed09ee3d8 26 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 6b5ad24ed09ee3d8 27 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 6b5ad24ed09ee3d8 28 we3w66dklTQz74q2RIi9koeC8Us (comment: C24 locus) fig2 ------- COMMENT: 6b5ad24ed09ee3d8 29 we3w66dklTQz74q2RIi9koeC8Us (comment: C24 locus) fig2 ------- COMMENT: 6b5ad24ed09ee3d8 46 hqMblxrEOC+68kxAobwk36RTrQs Figure 4b ------- COMMENT: 6b5ad24ed09ee3d8 47 qF5FW1GiCdOpvl68LCoZZJ88/CA Figure 4b (comment: live cell observation) ------- COMMENT: 6b5ad24ed09ee3d8 48 qF5FW1GiCdOpvl68LCoZZJ88/CA Figure 4b (comment: live cell observation) ------- COMMENT: 6b6f6a7ce48fd8e7 1 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 6b6f6a7ce48fd8e7 2 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 6b6f6a7ce48fd8e7 3 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 6b6f6a7ce48fd8e7 4 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 6b6f6a7ce48fd8e7 5 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 6b6f6a7ce48fd8e7 6 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 6b6f6a7ce48fd8e7 7 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 6b8ae0c3fd41751d 6 AiPSX5fmzqTaMumhp4EUgZswnhM However, GII activity is significantly reduced in the microsomal fraction of 􏰄GII􏰀 cells (Figure 2B), suggesting that GII􏰀 is involved in ER localization of GII􏰁 ------- COMMENT: 6b8ae0c3fd41751d 9 dwE4dptwRc5f6ur9N8xD4C9nvJc GII􏰀 Is Required for an Efficient In Vitro Glucose Trimming from G2M9 and G1M9 ------- COMMENT: 6b8ae0c3fd41751d 13 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 6b8ae0c3fd41751d 14 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 6b8ae0c3fd41751d 15 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 6b8ae0c3fd41751d 16 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: 6ba7e958410b1f95 1 w2SVaC8oyyVGzSg2ABCrXFuMmY4 Quantification of the signals revealed that Ago1 contained about 3-fold more antisense than sense ura4+ siRNAs. etc....(figure 1) ------- COMMENT: 6ba7e958410b1f95 2 uF2exANjHxM3JfWT4dpekn/x5GQ We found that deletion of cid14+ resulted in a complete loss of ura4+ silencing at all the tested loci as assayed by growth on 5-FOA-containing medium (Figures 2A–2C). ------- COMMENT: 6ba7e958410b1f95 3 uF2exANjHxM3JfWT4dpekn/x5GQ We found that deletion of cid14+ resulted in a complete loss of ura4+ silencing at all the tested loci as assayed by growth on 5-FOA-containing medium (Figures 2A–2C). ------- COMMENT: 6ba7e958410b1f95 4 uF2exANjHxM3JfWT4dpekn/x5GQ We found that deletion of cid14+ resulted in a complete loss of ura4+ silencing at all the tested loci as assayed by growth on 5-FOA-containing medium (Figures 2A–2C). ------- COMMENT: 6ba7e958410b1f95 5 YhntzqJfAx/K1xfAvXMFrO3IsXM Finally, deletion of the other four members of the fission yeast Cid14/Trf4/5 poly(A) polymerase family did not affect silencing of an imr1R::ura4+ reporter gene (Figure 2H). ------- COMMENT: 6ba7e958410b1f95 6 YhntzqJfAx/K1xfAvXMFrO3IsXM Finally, deletion of the other four members of the fission yeast Cid14/Trf4/5 poly(A) polymerase family did not affect silencing of an imr1R::ura4+ reporter gene (Figure 2H). ------- COMMENT: 6ba7e958410b1f95 7 YhntzqJfAx/K1xfAvXMFrO3IsXM Finally, deletion of the other four members of the fission yeast Cid14/Trf4/5 poly(A) polymerase family did not affect silencing of an imr1R::ura4+ reporter gene (Figure 2H). ------- COMMENT: 6ba7e958410b1f95 8 YhntzqJfAx/K1xfAvXMFrO3IsXM Finally, deletion of the other four members of the fission yeast Cid14/Trf4/5 poly(A) polymerase family did not affect silencing of an imr1R::ura4+ reporter gene (Figure 2H). ------- COMMENT: 6ba7e958410b1f95 9 tL4gmksBWkfszlc4/WS/tWx5Idw Surprisingly, in cid14D cells, neither Chp1 nor Swi6 binding was signifi- cantly reduced at several heterochromatic loci, includ- ing mat3M::ura4+, imr1R::ura4+, the subtelomeric tlh1+ gene, and cen-dg and cen-dh repeats, as assayed by chromatin immunoprecipitation experiments (ChIP) (Fig- ures 3A and 3B). ------- COMMENT: 6ba7e958410b1f95 10 tL4gmksBWkfszlc4/WS/tWx5Idw Surprisingly, in cid14D cells, neither Chp1 nor Swi6 binding was signifi- cantly reduced at several heterochromatic loci, includ- ing mat3M::ura4+, imr1R::ura4+, the subtelomeric tlh1+ gene, and cen-dg and cen-dh repeats, as assayed by chromatin immunoprecipitation experiments (ChIP) (Fig- ures 3A and 3B). ------- COMMENT: 6ba7e958410b1f95 11 tL4gmksBWkfszlc4/WS/tWx5Idw Surprisingly, in cid14D cells, neither Chp1 nor Swi6 binding was signifi- cantly reduced at several heterochromatic loci, includ- ing mat3M::ura4+, imr1R::ura4+, the subtelomeric tlh1+ gene, and cen-dg and cen-dh repeats, as assayed by chromatin immunoprecipitation experiments (ChIP) (Fig- ures 3A and 3B). ------- COMMENT: 6ba7e958410b1f95 12 SrgwQMoPNNEVO8DjP8jeqGC/Aps In addition, while H3K9 methylation was lost in clr4D cells, only a slight reduction in H3K9 methylation was observed in cid14D cells (Figures 3A and 3B) ------- COMMENT: 6ba7e958410b1f95 13 c3RZOcw9etlYcU73j5PjNGpoSq4 Furthermore, consistent with a CTGS model for silencing of mat3M::ura4+, none of the tested mutants af- fected RNApII occupancy at this locus (Figure 3C). ------- COMMENT: 6ba7e958410b1f95 14 UaHfMiuaj7fz8kgUFYvVXxIAj2g Cid14 copurified with two proteins that are homologs of Mtr4 and Air1 (Figures 4A and 4B). ------- COMMENT: 6ba7e958410b1f95 15 UaHfMiuaj7fz8kgUFYvVXxIAj2g Cid14 copurified with two proteins that are homologs of Mtr4 and Air1 (Figures 4A and 4B). ------- COMMENT: 6ba7e958410b1f95 16 NmyGL3IZ+sE47RblJPIViPD05PQ Cid14 copurified with two proteins that are homologs of Mtr4 and Air1 (Figures 4A and 4B). Thus, like Trf4 in S. cerevisiae, Cid14 is found in a complex together with Air1 and Mtr4, which we refer to as spTRAMP (S. pombe TRAMP). ------- COMMENT: 6ba7e958410b1f95 17 NmyGL3IZ+sE47RblJPIViPD05PQ Cid14 copurified with two proteins that are homologs of Mtr4 and Air1 (Figures 4A and 4B). Thus, like Trf4 in S. cerevisiae, Cid14 is found in a complex together with Air1 and Mtr4, which we refer to as spTRAMP (S. pombe TRAMP). ------- COMMENT: 6ba7e958410b1f95 18 NmyGL3IZ+sE47RblJPIViPD05PQ Cid14 copurified with two proteins that are homologs of Mtr4 and Air1 (Figures 4A and 4B). Thus, like Trf4 in S. cerevisiae, Cid14 is found in a complex together with Air1 and Mtr4, which we refer to as spTRAMP (S. pombe TRAMP). ------- COMMENT: 6ba7e958410b1f95 19 PQUHWEF+TX1GAziuGFRBYBMUVl4 We found that the deletion of air1+ had no effect on heterochromatic gene silencing (Figure S2 ------- COMMENT: 6ba7e958410b1f95 20 5GGn4hj0jZO5x2Rq6UNjevAdkVo However, we observed loss of silencing of mat3M:::ura4+ in cells carrying a hypomorphic allele of mtr4+ (mtr4-1, Figures 4F and 4H), suggesting the involvement of a TRAMP-like complex. ------- COMMENT: 6ba7e958410b1f95 21 4qj846wHpM9kHCj1gDMitZ28/R4 Consistent with a role for the exosome in degrad- ing heterochromatic ura4+ transcripts, we observed elevated ura4+ transcript levels in rrp6D compared to wild-type cells (Figures 4C–4E). ------- COMMENT: 6ba7e958410b1f95 22 Vr9imrZq/liaedo4YmdepDg0lIY figure 2 &4 ------- COMMENT: 6ba7e958410b1f95 23 Vr9imrZq/liaedo4YmdepDg0lIY figure 2 &4 ------- COMMENT: 6ba7e958410b1f95 24 Vr9imrZq/liaedo4YmdepDg0lIY figure 2 &4 ------- COMMENT: 6ba7e958410b1f95 25 T3jRI++D0o5ERCfzlBZiH0adf/4 we observed 7-fold and a 25-fold increases in tlh1+ transcript levels in rrp6D and dis3-54 cells, respectively, and 33- and 100- fold increases in cid14D and clr4D cells, respectively (Figures 4G and S3) ------- COMMENT: 6ba7e958410b1f95 26 T3jRI++D0o5ERCfzlBZiH0adf/4 we observed 7-fold and a 25-fold increases in tlh1+ transcript levels in rrp6D and dis3-54 cells, respectively, and 33- and 100- fold increases in cid14D and clr4D cells, respectively (Figures 4G and S3) ------- COMMENT: 6ba7e958410b1f95 27 T3jRI++D0o5ERCfzlBZiH0adf/4 we observed 7-fold and a 25-fold increases in tlh1+ transcript levels in rrp6D and dis3-54 cells, respectively, and 33- and 100- fold increases in cid14D and clr4D cells, respectively (Figures 4G and S3) ------- COMMENT: 6ba7e958410b1f95 28 T3jRI++D0o5ERCfzlBZiH0adf/4 we observed 7-fold and a 25-fold increases in tlh1+ transcript levels in rrp6D and dis3-54 cells, respectively, and 33- and 100- fold increases in cid14D and clr4D cells, respectively (Figures 4G and S3) ------- COMMENT: 6ba7e958410b1f95 29 UeXB/bGvuaMIvBtkUTvvTyHMn9s we observed a 3-fold increase in tlh1+ RNA levels in dcr1D cells and a 10-fold increase in mtr4-1 cells (Figures 4F and 4H), indicating that both RNAi and TRAMP contribute to the full silencing of this subtelomeric gene. ------- COMMENT: 6ba7e958410b1f95 30 UeXB/bGvuaMIvBtkUTvvTyHMn9s we observed a 3-fold increase in tlh1+ RNA levels in dcr1D cells and a 10-fold increase in mtr4-1 cells (Figures 4F and 4H), indicating that both RNAi and TRAMP contribute to the full silencing of this subtelomeric gene. ------- COMMENT: 6ba7e958410b1f95 31 IAoaBdN4ZHESFnF5TZKblbNQxPg In order to directly determine whether Cid14 is a bona fide poly(A) polymerase, we assayed recombinant wild-type or mutant Cid14 (GST-Cid14wt or GST-Cid14DADA, respec- tively; Figure 5A) for polyadenylation activity in vitro and found that wild-type Cid14 was able to extend a synthetic oligo(A)15 RNA but not an oligo(dA)15 DNA substrate (Figures 5B, 5C, and 5E). ------- COMMENT: 6ba7e958410b1f95 32 5IcQW6nV/3l7AT0vgprc4CujwwA Importantly, Cid14 activity was completely abolished in Cid14DADA (Figure 5B) ------- COMMENT: 6ba7e958410b1f95 33 TSwi/jK8Zq0cyzMPW5ioPMV+3X0 In order to directly determine whether Cid14 is a bona fide poly(A) polymerase, we assayed recombinant wild-type or mutant Cid14 (GST-Cid14wt or GST-Cid14DADA, respec- tively; Figure 5A) for polyadenylation activity in vitro and found that wild-type Cid14 was able to extend a synthetic oligo(A)15 RNAno ac- tivity was detected in the presence of pyrimidines (CTP or UTP) (Figure 5D). ------- COMMENT: 6ba7e958410b1f95 34 6DjJzIazGaJQZ65jf0tEEQA9k34 Loss of imr1R::ura4+ and mat3M::ura4+ silencing in cid14D cells could be rescued by overexpressing Cid14wt (pRep- Cid14) but not by Cid14DADA (pRep-Cid14DADA) (Fig- ure 5F). ------- COMMENT: 6ba7e958410b1f95 35 /NtwXlI8hOvrguU1+w1MVFdNYYE While we were not able to detect any centromeric siRNAs in cid12D cells, centromeric siRNAs were about 22-fold reduced in cid14D compared to wild-type cells (Figure 6B). and However, centromeric siRNAs from cid14D were barely detectable on total RNA northern blots (Figure 6A). ------- COMMENT: 6ba7e958410b1f95 36 ANc+Q/5ISHO/Cv3Ql2+s0mvNIGc Like cid14D, Cid14 active site mutations had dramatically reduced centromeric siRNA levels (Fig- ure 6D), ------- COMMENT: 6ba8570e85c749bc 20 CGLnXtpNndSnP7mrVUY71DS/7/4 (comment: Cdc45 reappears quickly after shift from restrictive to permissive temperature) ------- COMMENT: 6bede5008acef48c 114 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 6bede5008acef48c 116 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 6bede5008acef48c 117 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 6c1cc5fd9503d415 1 9lPNvAkKgJ5qQsV25U21SYfMOTs The Alp7-Alp14 complex localises to kinetochores prior to meiosis I independently of microtubules, which does not seem to occur in mitosis. ------- COMMENT: 6c1cc5fd9503d415 2 9lPNvAkKgJ5qQsV25U21SYfMOTs The Alp7-Alp14 complex localises to kinetochores prior to meiosis I independently of microtubules, which does not seem to occur in mitosis. ------- COMMENT: 6c1cc5fd9503d415 7 VDkjAM73wImuLxSOsyDnY1A2Cp8 The Nuf2 complex interacts with the Alp7-Alp14 complex phosphorylated by the polo kinase Plo1 ------- COMMENT: 6c1cc5fd9503d415 8 YnWhWQXGXAubPpMiWMsg3YPpcxY (comment: CHECK meiosis I) ------- COMMENT: 6c1cc5fd9503d415 9 88B/FF9PvhJN9wjH6WXmnFBBq5Q figure 2a ------- COMMENT: 6c1cc5fd9503d415 10 ouq88xK3qYy+YROPaqohtVfHs+0 fig 2g ------- COMMENT: 6c1cc5fd9503d415 11 pAevzwJai/4qy+x9a+uGKnjCBkM Fig 2 b-d (comment: CHECK involved in kinetochore retrieval during meiotic prophase) ------- COMMENT: 6c1cc5fd9503d415 13 Tie4qOAaRo48yvgdm5JSdaGSLJo Fig 2 b-d (comment: CHECK involved in kinetochore retrieval during meiotic prophase) ------- COMMENT: 6c1cc5fd9503d415 14 U5nmlofP95W0H1PkH2mLkZ9akjs fig 2G ------- COMMENT: 6c1cc5fd9503d415 15 mmvWVVqdqXM3rUn6NSbwwq0p0iw Fig 3b ------- COMMENT: 6c1cc5fd9503d415 16 W4egHp8nZtd5iNYpwU05IAEyjwY fig 3d ------- COMMENT: 6c1cc5fd9503d415 17 W4egHp8nZtd5iNYpwU05IAEyjwY fig 3d ------- COMMENT: 6c1cc5fd9503d415 18 KksxtxDafY9Uv5u//pt/gjSIkEo (comment: CHECK Is this phase correct?) ------- COMMENT: 6c1cc5fd9503d415 19 KksxtxDafY9Uv5u//pt/gjSIkEo (comment: CHECK Is this phase correct?) ------- COMMENT: 6c1cc5fd9503d415 20 s7cvhfiYiveaAA2KzWLwMqq+fAs fig 4c ------- COMMENT: 6c1cc5fd9503d415 21 s7cvhfiYiveaAA2KzWLwMqq+fAs fig 4c ------- COMMENT: 6c1cc5fd9503d415 22 c0uMiNqcE3BgpszPax9cHtCbVbg fig 4d ------- COMMENT: 6c1cc5fd9503d415 23 c0uMiNqcE3BgpszPax9cHtCbVbg fig 4d ------- COMMENT: 6c1cc5fd9503d415 24 jc/ZDB7Nq+yNz/h3c2bU4aWYEvk figure 4c ------- COMMENT: 6c1cc5fd9503d415 26 fYqQOpzikopc+3AWAEPEUkYII7w figure 4 ------- COMMENT: 6c1cc5fd9503d415 27 4yCVBSkl6bg2aTrDdCZ1ZS1USlw fig 5b ------- COMMENT: 6c1cc5fd9503d415 28 b6GZtZ+LOqEk1uWUQUHcReNdCds Fig 5. d,e (comment: CHECK unattached) ------- COMMENT: 6c1cc5fd9503d415 29 4yCVBSkl6bg2aTrDdCZ1ZS1USlw fig 5b ------- COMMENT: 6c1cc5fd9503d415 30 /MeFOUj/tH9NgUX9Yb5pgdGWrFE Fig 6a ------- COMMENT: 6c1cc5fd9503d415 31 EVJOiRv1ppVpuJuHNikV8GSqjkY Figure 6 b/d (comment: d used chromosome tethered polo mutants, I didn't curate these phenotypes) ------- COMMENT: 6c1cc5fd9503d415 32 KksxtxDafY9Uv5u//pt/gjSIkEo (comment: CHECK Is this phase correct) ------- COMMENT: 6c1cc5fd9503d415 33 VfWhAtCIfE3AokG4g3+X1vXHDNw (comment: polo consensus) fig 6b ------- COMMENT: 6c1cc5fd9503d415 34 tC0NAi10z8NhERGAwcayOJj6jAE Supp Fig S6 ------- COMMENT: 6c1cc5fd9503d415 35 tC0NAi10z8NhERGAwcayOJj6jAE Supp Fig S6 ------- COMMENT: 6c1cc5fd9503d415 36 K4v9g4p20PbfbosqRA5YMBpAF1Y Fig 6c (comment: CHeCK or abolished) ------- COMMENT: 6c1cc5fd9503d415 37 TBMsUvnlYcDpEmeGUAH2Kz1ylZ4 Fig 7 ------- COMMENT: 6c1cc5fd9503d415 38 w2rZhBY+lrShUOkRV1+hUc6Gpg8 fig 7c ------- COMMENT: 6c1cc5fd9503d415 39 9lPNvAkKgJ5qQsV25U21SYfMOTs The Alp7-Alp14 complex localises to kinetochores prior to meiosis I independently of microtubules, which does not seem to occur in mitosis. ------- COMMENT: 6c1cc5fd9503d415 46 VDkjAM73wImuLxSOsyDnY1A2Cp8 The Nuf2 complex interacts with the Alp7-Alp14 complex phosphorylated by the polo kinase Plo1 ------- COMMENT: 6c335a4e1d1a262d 2 bdaWELkvx4jiwYUzmHUiIIRo2ig Fig1A BrdU incorporation wee1-50 strain analysed at 32°C ------- COMMENT: 6c335a4e1d1a262d 3 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: 6c335a4e1d1a262d 8 q6RbUe9PFR/arS8NPy7Iq1ZwhY4 Fig1G amount of tos1-GFP in nucleus is dependent on cdc10 ------- COMMENT: 6c335a4e1d1a262d 18 MxuKyZ67/KyR/Q31rOpFscaqVJg section titled MBF-dependent gene expression...these cells undergo G1 transcription, a seemingly normal Sphase (no region specific amplifications) and can only reinitiate replication once size per genome is minimal size. ------- COMMENT: 6c335a4e1d1a262d 19 s8zxygB4j9+cKrsniSSlYhY+9P0 Fig5A ------- COMMENT: 6c6733a743354788 1 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 6c6733a743354788 2 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 6c6733a743354788 3 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 6c6733a743354788 4 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 6c6733a743354788 5 MuQlumEsMRN8H0qv4jNwRvRTQ0g Fig 2, S2 ------- COMMENT: 6c6733a743354788 6 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 6c6733a743354788 7 W54/y9rG1OOCMJe2liPNjvR7ZW8 Fig 3 (comment: less intense Y arc in 2D gel) ------- COMMENT: 6c6733a743354788 8 W54/y9rG1OOCMJe2liPNjvR7ZW8 Fig 3 (comment: less intense Y arc in 2D gel) ------- COMMENT: 6c6733a743354788 9 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 6c6733a743354788 10 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 6c6733a743354788 11 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 6c6733a743354788 12 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 6c6733a743354788 13 2ssFNJE05JceDZCrlAudQM6sB0g Fig S1 ------- COMMENT: 6c6733a743354788 14 2ssFNJE05JceDZCrlAudQM6sB0g Fig S1 ------- COMMENT: 6c6733a743354788 15 JzyBKOIiyuH2U/GwaoWxC6/fJhQ Fig S2 ------- COMMENT: 6c6733a743354788 16 JzyBKOIiyuH2U/GwaoWxC6/fJhQ Fig S2 ------- COMMENT: 6c6733a743354788 17 L80SI+C51Oud3kemGrbAUeWd2W8 Fig EV2 ------- COMMENT: 6c6733a743354788 18 JzyBKOIiyuH2U/GwaoWxC6/fJhQ Fig S2 ------- COMMENT: 6c6733a743354788 19 JzyBKOIiyuH2U/GwaoWxC6/fJhQ Fig S2 ------- COMMENT: 6c6733a743354788 20 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 6c6733a743354788 21 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 6c6733a743354788 22 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 6c6733a743354788 23 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 6c6733a743354788 24 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 6c6733a743354788 25 tEKPG4SG06Sd+MBgYUZdTXf2msI Fig S3 ------- COMMENT: 6c6733a743354788 26 tEKPG4SG06Sd+MBgYUZdTXf2msI Fig S3 ------- COMMENT: 6c6733a743354788 27 tEKPG4SG06Sd+MBgYUZdTXf2msI Fig S3 ------- COMMENT: 6c6733a743354788 28 tEKPG4SG06Sd+MBgYUZdTXf2msI Fig S3 ------- COMMENT: 6c6733a743354788 29 tEKPG4SG06Sd+MBgYUZdTXf2msI Fig S3 ------- COMMENT: 6c6733a743354788 30 tEKPG4SG06Sd+MBgYUZdTXf2msI Fig S3 ------- COMMENT: 6c6733a743354788 31 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 6c6733a743354788 32 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 6c6733a743354788 33 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 6c6733a743354788 34 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 6c6733a743354788 35 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 6c6733a743354788 36 2TM0hbGD5RT5C4BsmHZVHFY51OA Fig EV3 ------- COMMENT: 6c6733a743354788 37 2TM0hbGD5RT5C4BsmHZVHFY51OA Fig EV3 ------- COMMENT: 6c6733a743354788 38 bW9hQ6X4na6lmbQk9RFl9Bur7Fg Fig EV3; restrictive temperature for cdc2-M68 ------- COMMENT: 6c6733a743354788 39 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 6c6733a743354788 40 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 6c6733a743354788 43 yqpfVTXfvxfP8xk888fc4w/qRH4 (comment: also inferred from orthology and various genetic interactions) ------- COMMENT: 6c6a9502b98a3bdb 95 L2gFFiSyr1ahKhxW1LfsaLaygos (comment: cDNA; no introns) ------- COMMENT: 6c6a9502b98a3bdb 98 L2gFFiSyr1ahKhxW1LfsaLaygos (comment: cDNA; no introns) ------- COMMENT: 6c6a9502b98a3bdb 99 L2gFFiSyr1ahKhxW1LfsaLaygos (comment: cDNA; no introns) ------- COMMENT: 6c6a9502b98a3bdb 101 L2gFFiSyr1ahKhxW1LfsaLaygos (comment: cDNA; no introns) ------- COMMENT: 6c6a9502b98a3bdb 128 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 6c6dece2e0762651 3 eM68CvXen3+KIwU789qQ9epzfJQ when wtf13 antidote not present (homozygous, wtf13poison/wtf13+, or wtf13poison/wtf13Δ ------- COMMENT: 6c6dece2e0762651 7 3MSWsdOf2TR3z5vcr/4bEKqevJ4 ((comment: suppressed by wtf18-2 allele) ------- COMMENT: 6c6dece2e0762651 8 GEJX1kOnDXrrApLjp0A1b+EpNng (comment: suppresses wtf13 drive) ------- COMMENT: 6c6dece2e0762651 10 vBkBuU56fOgz4mSoLTW/2J3SWKw (comment: wtf13 driver, wtf18 suppressor) ------- COMMENT: 6c6dece2e0762651 11 vBkBuU56fOgz4mSoLTW/2J3SWKw (comment: wtf13 driver, wtf18 suppressor) ------- COMMENT: 6c6dece2e0762651 12 vBkBuU56fOgz4mSoLTW/2J3SWKw (comment: wtf13 driver, wtf18 suppressor) ------- COMMENT: 6c6dece2e0762651 13 vBkBuU56fOgz4mSoLTW/2J3SWKw (comment: wtf13 driver, wtf18 suppressor) ------- COMMENT: 6c6dece2e0762651 15 h3VfonSGfQjdV559n6vvqbNDQVY (comment: wtf18-2 allele assayed) ------- COMMENT: 6c783779bf55e958 21 8JpLSphP2pmmXS6xwpdWX5EQcJ4 (comment: mah: CHECK FYPO:0001178 + PECO:0000240 captures info for requested new term (Term name: loss of viability upon long-term nutrient starvation Definition: A cell population phenotype in which a smaller than normal proportion of the population remains viable when cells in a culture in stationary phase are deprived of nitrogen. Use this term to annotate experiments in which a culture is cultivated in stationary phase under nitrogen-depleted conditions for a long time (more than 1 week), and then the number of cells viable enough to form a colony upon return to conditions supporting vegetative growth is measured and compared to wild type) ------- COMMENT: 6c7fa98f457520e1 46 Nvj6aD3CNPjB08Q5I2pfFIqqo1E (comment: after 100 generations) ------- COMMENT: 6cdebcc5bb8f2240 1 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 6cdebcc5bb8f2240 2 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 6cdebcc5bb8f2240 3 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 6cdebcc5bb8f2240 4 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 6cdebcc5bb8f2240 5 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 6cdebcc5bb8f2240 6 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 6cdebcc5bb8f2240 7 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 6d1a7154e047457b 3 ec6Pexk3YIDQbVlZSI+qHvnsa4A (comment: The phenotype is assessed by the high-throughput sequencing) ------- COMMENT: 6d1a7154e047457b 7 ec6Pexk3YIDQbVlZSI+qHvnsa4A (comment: The phenotype is assessed by the high-throughput sequencing) ------- COMMENT: 6d1a7154e047457b 8 ec6Pexk3YIDQbVlZSI+qHvnsa4A (comment: The phenotype is assessed by the high-throughput sequencing) ------- COMMENT: 6d1a7154e047457b 9 ec6Pexk3YIDQbVlZSI+qHvnsa4A (comment: The phenotype is assessed by the high-throughput sequencing) ------- COMMENT: 6d1f3a3caef131ad 6 lp2ySbXHN5XzEznRwAePTeyhYb4 Figure 2, Figure 3. ------- COMMENT: 6d1f3a3caef131ad 7 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 6d1f3a3caef131ad 8 bDuFvlon21TIwyeVb8pCdPAKpkE Figure 2, Figure 3 ------- COMMENT: 6d1f3a3caef131ad 9 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 6d1f3a3caef131ad 11 W8U2HOh5c9FHrXYcx0iyvAr6hdQ Figure 7A ------- COMMENT: 6d1f3a3caef131ad 12 jaXSMkIkUFbR7xqB4F/n82m0ucQ Figure 7C, 7D ------- COMMENT: 6d1f3a3caef131ad 13 jaXSMkIkUFbR7xqB4F/n82m0ucQ Figure 7C, 7D ------- COMMENT: 6d1f3a3caef131ad 14 W8U2HOh5c9FHrXYcx0iyvAr6hdQ Figure 7A ------- COMMENT: 6d1f3a3caef131ad 15 W8U2HOh5c9FHrXYcx0iyvAr6hdQ Figure 7A ------- COMMENT: 6d1f3a3caef131ad 16 jaXSMkIkUFbR7xqB4F/n82m0ucQ Figure 7C, 7D ------- COMMENT: 6d1f3a3caef131ad 17 jaXSMkIkUFbR7xqB4F/n82m0ucQ Figure 7C, 7D ------- COMMENT: 6d1f3a3caef131ad 18 W8U2HOh5c9FHrXYcx0iyvAr6hdQ Figure 7A ------- COMMENT: 6d1f3a3caef131ad 19 sRbiU/jtYRJxVkHoyWOhqsXRQaU Figure S5 ------- COMMENT: 6d218019d6c51a94 1 sStPLoNgUrUZCzBKk2BqTrlYILM establishment, but not maintenance, of ring localization requires F-actin (assayed using latrunculin A) ------- COMMENT: 6d218019d6c51a94 2 sStPLoNgUrUZCzBKk2BqTrlYILM establishment, but not maintenance, of ring localization requires F-actin (assayed using latrunculin A) ------- COMMENT: 6d4a9d65ed297cda 4 K0LN81c8Q9qRMXfG3I0XLvHlLrI Fig 1A growth inhibited by 0.005% MMS after 4 days ------- COMMENT: 6d4a9d65ed297cda 5 deSjNYYl8y1X17QvhYnniT1d+dM Fig 2 C (comment: RTS1-RFB assay) ------- COMMENT: 6d4a9d65ed297cda 6 haCpKk/J7+AvwVw7MsoYizTayUU The fft3-K418R-myc strain exhibited similar sensitivity to CPT and MMS than fft3Δ cells, indicating that the ATPase activity is required to promote cell re- sistance to replication stress. ------- COMMENT: 6d4a9d65ed297cda 7 u0vN6QKKPo72TTLD4G/5mnqcA+s (comment: RTS1-RFB assay) ------- COMMENT: 6d4a9d65ed297cda 8 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 6d4a9d65ed297cda 9 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 6d4a9d65ed297cda 10 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 6d4a9d65ed297cda 12 5Soo3460P0EP5KXftpJqxzqHuIs (comment: constitutive) ------- COMMENT: 6d4a9d65ed297cda 13 J/RB4adPtRAK7JmCD9Rv2hpbAHA decreased replciation restart fig1 indicating that only one-third of forks arrested at the RTS1-RFB are efficiently restarted in the absence of Fft3. ------- COMMENT: 6d4a9d65ed297cda 14 Jge7gzQ/vEwtRcbstp7MgEwVmUM normal replciation restart/ HR-mediated fork restart RTS1-RFB assay. urprisingly, the induction of downstream RS in fft3-K418R-myc strain was similar to the one observed in wild-type cells (Fig 4D, bottom panel). This finding indicates that the lack of the ATPase activity does not impact the efficiency of HR-mediated fork restart. ------- COMMENT: 6d702a6f1957248f 1 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 6d702a6f1957248f 2 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 6d702a6f1957248f 3 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 6d702a6f1957248f 4 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 6d702a6f1957248f 5 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 6d702a6f1957248f 6 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 6d702a6f1957248f 7 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 6d702a6f1957248f 8 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 6d702a6f1957248f 9 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 6d702a6f1957248f 10 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6d702a6f1957248f 11 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6d702a6f1957248f 12 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6d702a6f1957248f 13 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6d702a6f1957248f 14 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6d702a6f1957248f 15 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6d702a6f1957248f 16 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6d702a6f1957248f 17 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6d702a6f1957248f 18 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6d702a6f1957248f 19 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6d702a6f1957248f 20 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6d702a6f1957248f 21 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6d702a6f1957248f 22 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6d702a6f1957248f 23 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6d702a6f1957248f 24 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6d702a6f1957248f 25 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6d702a6f1957248f 26 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6d702a6f1957248f 27 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6d702a6f1957248f 28 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6d702a6f1957248f 29 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6d702a6f1957248f 30 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 6d702a6f1957248f 31 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6d702a6f1957248f 32 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6d702a6f1957248f 33 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6d702a6f1957248f 34 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6d702a6f1957248f 35 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6d702a6f1957248f 36 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6d702a6f1957248f 37 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6d702a6f1957248f 38 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6d702a6f1957248f 39 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6d702a6f1957248f 40 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6d702a6f1957248f 41 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6d702a6f1957248f 42 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6d702a6f1957248f 43 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6d702a6f1957248f 44 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6d702a6f1957248f 45 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6d702a6f1957248f 46 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6d702a6f1957248f 47 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6d702a6f1957248f 48 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6d702a6f1957248f 49 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6d702a6f1957248f 50 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6d702a6f1957248f 51 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 6d702a6f1957248f 52 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 6d702a6f1957248f 53 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 6d702a6f1957248f 54 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 6d702a6f1957248f 55 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 6d702a6f1957248f 56 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 6d702a6f1957248f 57 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 6d702a6f1957248f 58 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 6d702a6f1957248f 59 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 6d702a6f1957248f 60 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: 6d702a6f1957248f 61 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: 6d702a6f1957248f 62 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 6d702a6f1957248f 63 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 6d702a6f1957248f 64 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 6d702a6f1957248f 65 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 6d702a6f1957248f 66 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 6d702a6f1957248f 67 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 6d702a6f1957248f 68 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 6d702a6f1957248f 69 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 6d702a6f1957248f 70 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 6d702a6f1957248f 71 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 6d702a6f1957248f 72 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 6d702a6f1957248f 73 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 6d702a6f1957248f 74 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 6d702a6f1957248f 75 lt2pkx5XPTUurrYIIsqW8bTRKF8 Fig. 7C ------- COMMENT: 6d702a6f1957248f 76 lt2pkx5XPTUurrYIIsqW8bTRKF8 Fig. 7C ------- COMMENT: 6d702a6f1957248f 77 3Fo3nF7k/Ug4diSSuVVVLaHD90s These results indicate that RITS and Atf1-Pcr1 participate in distinct and parallel pathways to nucleate heterochromatin at the mat locus. ------- COMMENT: 6d702a6f1957248f 78 3Fo3nF7k/Ug4diSSuVVVLaHD90s These results indicate that RITS and Atf1-Pcr1 participate in distinct and parallel pathways to nucleate heterochromatin at the mat locus. ------- COMMENT: 6d702a6f1957248f 79 3Fo3nF7k/Ug4diSSuVVVLaHD90s These results indicate that RITS and Atf1-Pcr1 participate in distinct and parallel pathways to nucleate heterochromatin at the mat locus. ------- COMMENT: 6dc480135e507182 19 48/jGBWfgvr9UOhIRBYMTqb6UTE (comment: also L-gamma-glutamyl-L-cysteine) ------- COMMENT: 6dda6bff1b0363f6 1 +tzRKybcMm83cK/ndZ5rmMRVPJU Required for phosphatydil serine reorganization at the inner leaflet of plasma membrande during cell fusion ------- COMMENT: 6dda6bff1b0363f6 22 HB4Fge7ZGZMiiaGMZY1O4r3Uzpk (comment: CHECK high penetrance) ------- COMMENT: 6dda6bff1b0363f6 56 NKbVEw5untp77BVAqkTHmm4bESI (comment: CHECK low expressivity) ------- COMMENT: 6df01632fc970fbe 2 OqxJwZuCNI13yFG/V6HP/eAasZ4 (comment: assayed using GFP reporter with or without premature stop codons) ------- COMMENT: 6df01632fc970fbe 4 OqxJwZuCNI13yFG/V6HP/eAasZ4 (comment: assayed using GFP reporter with or without premature stop codons) ------- COMMENT: 6df01632fc970fbe 6 oAj7p++JxyAGFT8LJt+9GKesE0U (comment: assayed using ypt3 reporter with or without premature stop codons) ------- COMMENT: 6df01632fc970fbe 8 oAj7p++JxyAGFT8LJt+9GKesE0U (comment: assayed using ypt3 reporter with or without premature stop codons) ------- COMMENT: 6dfef8615eb3cdaa 26 RPEYaixAqptl/z5KU50n5oD5WIU (comment: mei2 promotes g1 arrest, premeiotic dna replication and meiosis I) ------- COMMENT: 6e175411383ea731 21 8nfTuAzCheSZ8UdoDWpWsW3UQys fig 2a (comment: CHECK no spindle rod like chromsomes) ------- COMMENT: 6e175411383ea731 22 8nfTuAzCheSZ8UdoDWpWsW3UQys fig 2a (comment: no spindle rod like chromsomes) ------- COMMENT: 6e175411383ea731 23 xSgq/1PyvS+43QiBpmVp7HA6Uvw fig 2a ------- COMMENT: 6e175411383ea731 24 coBTmtFSwuxkBMiY8P2UDllndpE Fig 2b ------- COMMENT: 6e175411383ea731 25 8nfTuAzCheSZ8UdoDWpWsW3UQys fig 2a (comment: CHECK no spindle rod like chromsomes) ------- COMMENT: 6e175411383ea731 26 Wn6TFpW8oRYpHKztATTLG+AQkSs fig 2b ((comment: CHECK uncondensed chromosomes) ------- COMMENT: 6e175411383ea731 27 zKxgyRAsYgWL7f58cuMZpRjtV+I fig 2B ------- COMMENT: 6e175411383ea731 28 Wn6TFpW8oRYpHKztATTLG+AQkSs fig 2b (comment: CHECK uncondensed chromosomes) ------- COMMENT: 6e175411383ea731 29 gm1Px94b5UBdjxqoHSq63NZdxOQ table1 ------- COMMENT: 6e175411383ea731 30 gm1Px94b5UBdjxqoHSq63NZdxOQ table1 ------- COMMENT: 6e175411383ea731 31 gm1Px94b5UBdjxqoHSq63NZdxOQ table1 ------- COMMENT: 6e2ab1dbcb345a0f 1 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 6e2ab1dbcb345a0f 2 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 6e2ab1dbcb345a0f 5 mKHrmdoHpr4rPKS2pKRyXvNdG7s Fig. S6 ------- COMMENT: 6e2ab1dbcb345a0f 6 mKHrmdoHpr4rPKS2pKRyXvNdG7s Fig. S6 ------- COMMENT: 6e2ab1dbcb345a0f 7 mKHrmdoHpr4rPKS2pKRyXvNdG7s Fig. S6 ------- COMMENT: 6e2ab1dbcb345a0f 8 mKHrmdoHpr4rPKS2pKRyXvNdG7s Fig. S6 ------- COMMENT: 6e2ab1dbcb345a0f 9 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 6e2ab1dbcb345a0f 10 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 6e2ab1dbcb345a0f 11 h8WWDxqyE11+2Ku4VRtm9AbY22k Fig. S5A ------- COMMENT: 6e2ab1dbcb345a0f 12 h8WWDxqyE11+2Ku4VRtm9AbY22k Fig. S5A ------- COMMENT: 6e2ab1dbcb345a0f 13 Zq2Qgvwbtq3yBSVmITg7uCIJC9M Fig. S5B ------- COMMENT: 6e2ab1dbcb345a0f 14 Zq2Qgvwbtq3yBSVmITg7uCIJC9M Fig. S5B ------- COMMENT: 6e2ab1dbcb345a0f 15 Zq2Qgvwbtq3yBSVmITg7uCIJC9M Fig. S5B ------- COMMENT: 6e2ab1dbcb345a0f 16 Zq2Qgvwbtq3yBSVmITg7uCIJC9M Fig. S5B ------- COMMENT: 6e49b33e9e6d3a5f 2 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: 6e49b33e9e6d3a5f 3 RaPiJeE4LAzmUvVItFnpkPMvmGY figure 3b ------- COMMENT: 6e49b33e9e6d3a5f 4 rkl4aDNZWsufXiagWLR2mLeKa3Q Because the same distribution pattern was observed when nmt-GFP-13g6 was expressedin the klp3 null allele (Figure 3B), we conclude that ER distribution is not affected by the disruption of klp3 in Fission yeast. ------- COMMENT: 6e49b33e9e6d3a5f 8 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: 6e4bd6933c454826 1 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 6e4bd6933c454826 2 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 6e4bd6933c454826 3 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 6e4bd6933c454826 4 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 6e4bd6933c454826 5 QxyA7Kxt4u0SZftovXPYBddoxhU Fig 1D ------- COMMENT: 6e4bd6933c454826 6 QxyA7Kxt4u0SZftovXPYBddoxhU Fig 1D ------- COMMENT: 6e4bd6933c454826 7 QxyA7Kxt4u0SZftovXPYBddoxhU Fig 1D ------- COMMENT: 6e4bd6933c454826 8 QxyA7Kxt4u0SZftovXPYBddoxhU Fig 1D ------- COMMENT: 6e4bd6933c454826 9 QxyA7Kxt4u0SZftovXPYBddoxhU Fig 1D ------- COMMENT: 6e4bd6933c454826 10 YiR+IBb1kniED4g2sWxwBTTam3E HULC revealed that slower migrating band representing ubH2B was missing in cells lacking either Bre1 homologs (i.e. Rfp1 or Rfp2) or Shf1. These analyses suggest that components of HULC are required for ubiquitination of H2B. ------- COMMENT: 6e4bd6933c454826 11 YiR+IBb1kniED4g2sWxwBTTam3E HULC revealed that slower migrating band representing ubH2B was missing in cells lacking either Bre1 homologs (i.e. Rfp1 or Rfp2) or Shf1. These analyses suggest that components of HULC are required for ubiquitination of H2B. ------- COMMENT: 6e4bd6933c454826 14 VKHYOuJqPgB7JvDiTVDbckmnvM8 figure 2b ------- COMMENT: 6e4bd6933c454826 15 VKHYOuJqPgB7JvDiTVDbckmnvM8 figure 2b ------- COMMENT: 6e4bd6933c454826 16 VKHYOuJqPgB7JvDiTVDbckmnvM8 figure 2b ------- COMMENT: 6e4bd6933c454826 17 VKHYOuJqPgB7JvDiTVDbckmnvM8 figure 2b ------- COMMENT: 6e4bd6933c454826 18 VKHYOuJqPgB7JvDiTVDbckmnvM8 figure 2b ------- COMMENT: 6e4bd6933c454826 19 VrYcuhffXpcQvsPZp3GqcYoFh7Q figure 2d 􏰀rhp6 resulted in enhanced silencing of the otr1::ura4􏰁, as shown by reduced growth on medium lacking uracil (Fig. 2D) ------- COMMENT: 6e4bd6933c454826 20 VrYcuhffXpcQvsPZp3GqcYoFh7Q figur 2d 􏰀rhp6 resulted in enhanced silencing of the otr1::ura4􏰁, as shown by reduced growth on medium lacking uracil (Fig. 2D) ------- COMMENT: 6e4bd6933c454826 21 VrYcuhffXpcQvsPZp3GqcYoFh7Q figur 2d 􏰀rhp6 resulted in enhanced silencing of the otr1::ura4􏰁, as shown by reduced growth on medium lacking uracil (Fig. 2D) ------- COMMENT: 6e4bd6933c454826 22 VrYcuhffXpcQvsPZp3GqcYoFh7Q figur 2d 􏰀rhp6 resulted in enhanced silencing of the otr1::ura4􏰁, as shown by reduced growth on medium lacking uracil (Fig. 2D) ------- COMMENT: 6e4bd6933c454826 23 hDPdDoFWYq5SXkHJ6MukWq/1x7Q Overexpression of Rhp6 abro- gates silencing of the otr1::ura4􏰁 reporter, resulting in the loss of cell viability on medium supple- mented with FOA (Fig. 3A) ------- COMMENT: 6e4bd6933c454826 25 GE+d9JqOXRUcwJ7qvQDn7EYQ3h8 Interestingly, levels of trim- ethylated H3K9 (H3K9me3) were significantly reduced, although the levels of monomethylated H3K9 (H3K9me1) were increased at the dg repeat element and otr1::ura4􏰁 (Fig. 3B). ------- COMMENT: 6e4bd6933c454826 26 GE+d9JqOXRUcwJ7qvQDn7EYQ3h8 Interestingly, levels of trim- ethylated H3K9 (H3K9me3) were significantly reduced, although the levels of monomethylated H3K9 (H3K9me1) were increased at the dg repeat element and otr1::ura4􏰁 (Fig. 3B). ------- COMMENT: 6e4bd6933c454826 27 uKl4ijynWB8fpUQkfe+SqvdIazA (supplemental Fig. 2) ------- COMMENT: 6e4bd6933c454826 28 7cbi9wm0cwYeupDHHOWWCgupQJ4 Fig. 4A,B ------- COMMENT: 6e4bd6933c454826 29 7cbi9wm0cwYeupDHHOWWCgupQJ4 Fig. 4A,B ------- COMMENT: 6e4bd6933c454826 30 7cbi9wm0cwYeupDHHOWWCgupQJ4 Fig. 4A,B ------- COMMENT: 6e4bd6933c454826 31 7cbi9wm0cwYeupDHHOWWCgupQJ4 Fig. 4A,B ------- COMMENT: 6e4bd6933c454826 32 7cbi9wm0cwYeupDHHOWWCgupQJ4 Fig. 4A,B ------- COMMENT: 6e4bd6933c454826 33 7cbi9wm0cwYeupDHHOWWCgupQJ4 Fig. 4A,B ------- COMMENT: 6e4bd6933c454826 34 qlk6CRDxYAROXQxQWXaxWcc2Svc Interestingly, the H2B-K119R mutation sig- nificantly enhanced silencing of the otr1::ura4􏰁 (Fig. 5A), ------- COMMENT: 6e4bd6933c454826 35 +8KlaMrbC9Ein2bFXjX3ccRmUQw fig 5D ------- COMMENT: 6e4bd6933c454826 36 +8KlaMrbC9Ein2bFXjX3ccRmUQw fig 5D ------- COMMENT: 6e5ece46806fb848 2 7N2ouPXTxT4pB727+61TFdgcIYA (comment: Recombination assay; assayed region: leu1-his5 interval) ------- COMMENT: 6e5ece46806fb848 3 urmGXtTAv31GW4SNZKgE0auOWS8 (comment: mbs1 hotspot quantification) ------- COMMENT: 6e5ece46806fb848 5 iJIuBWNOeET7eUe2LwhPSvObyD4 (comment: Recombination assay; assayed region: ade6 gene) ------- COMMENT: 6e5ece46806fb848 6 7N2ouPXTxT4pB727+61TFdgcIYA (comment: Recombination assay; assayed region: leu1-his5 interval) ------- COMMENT: 6e5ece46806fb848 7 urmGXtTAv31GW4SNZKgE0auOWS8 (comment: mbs1 hotspot quantification) ------- COMMENT: 6e5ece46806fb848 8 iJIuBWNOeET7eUe2LwhPSvObyD4 (comment: Recombination assay; assayed region: ade6 gene) ------- COMMENT: 6e5ece46806fb848 9 7N2ouPXTxT4pB727+61TFdgcIYA (comment: Recombination assay; assayed region: leu1-his5 interval) ------- COMMENT: 6e5ece46806fb848 10 urmGXtTAv31GW4SNZKgE0auOWS8 (comment: mbs1 hotspot quantification) ------- COMMENT: 6e5ece46806fb848 11 urmGXtTAv31GW4SNZKgE0auOWS8 (comment: mbs1 hotspot quantification) ------- COMMENT: 6e5ece46806fb848 12 bz/HvCBRQFICmocPK4MHPQoi184 (comment: Rec25 visualization) ------- COMMENT: 6e5ece46806fb848 13 bz/HvCBRQFICmocPK4MHPQoi184 (comment: Rec25 visualization) ------- COMMENT: 6e5ece46806fb848 14 R/MmNyY2Hzemu3Omq2nkDMSWpg0 (comment: Cellular fractionation; affecting Rec25) ------- COMMENT: 6e5ece46806fb848 15 R/MmNyY2Hzemu3Omq2nkDMSWpg0 (comment: Cellular fractionation; affecting Rec25) ------- COMMENT: 6e5ece46806fb848 17 urmGXtTAv31GW4SNZKgE0auOWS8 (comment: mbs1 hotspot quantification) ------- COMMENT: 6e5ece46806fb848 18 iJIuBWNOeET7eUe2LwhPSvObyD4 (comment: Recombination assay; assayed region: ade6 gene) ------- COMMENT: 6e5ece46806fb848 19 7N2ouPXTxT4pB727+61TFdgcIYA (comment: Recombination assay; assayed region: leu1-his5 interval) ------- COMMENT: 6e5ece46806fb848 20 urmGXtTAv31GW4SNZKgE0auOWS8 (comment: mbs1 hotspot quantification) ------- COMMENT: 6e5ece46806fb848 21 bz/HvCBRQFICmocPK4MHPQoi184 (comment: Rec25 visualization) ------- COMMENT: 6e5ece46806fb848 22 R/MmNyY2Hzemu3Omq2nkDMSWpg0 (comment: Cellular fractionation; affecting Rec25) ------- COMMENT: 6e5ece46806fb848 25 iJIuBWNOeET7eUe2LwhPSvObyD4 (comment: Recombination assay; assayed region: ade6 gene) ------- COMMENT: 6e5ece46806fb848 26 iJIuBWNOeET7eUe2LwhPSvObyD4 (comment: Recombination assay; assayed region: ade6 gene) ------- COMMENT: 6e5ece46806fb848 28 iJIuBWNOeET7eUe2LwhPSvObyD4 (comment: Recombination assay; assayed region: ade6 gene) ------- COMMENT: 6e5ece46806fb848 29 iJIuBWNOeET7eUe2LwhPSvObyD4 (comment: Recombination assay; assayed region: ade6 gene) ------- COMMENT: 6e5ece46806fb848 30 iJIuBWNOeET7eUe2LwhPSvObyD4 (comment: Recombination assay; assayed region: ade6 gene) ------- COMMENT: 6e5ece46806fb848 31 iJIuBWNOeET7eUe2LwhPSvObyD4 (comment: Recombination assay; assayed region: ade6 gene) ------- COMMENT: 6e5ece46806fb848 32 iJIuBWNOeET7eUe2LwhPSvObyD4 (comment: Recombination assay; assayed region: ade6 gene) ------- COMMENT: 6e5ece46806fb848 33 iJIuBWNOeET7eUe2LwhPSvObyD4 (comment: Recombination assay; assayed region: ade6 gene) ------- COMMENT: 6e5ece46806fb848 34 iJIuBWNOeET7eUe2LwhPSvObyD4 (comment: Recombination assay; assayed region: ade6 gene) ------- COMMENT: 6e5ece46806fb848 35 iJIuBWNOeET7eUe2LwhPSvObyD4 (comment: Recombination assay; assayed region: ade6 gene) ------- COMMENT: 6e5ece46806fb848 36 iJIuBWNOeET7eUe2LwhPSvObyD4 (comment: Recombination assay; assayed region: ade6 gene) ------- COMMENT: 6e5ece46806fb848 37 iJIuBWNOeET7eUe2LwhPSvObyD4 (comment: Recombination assay; assayed region: ade6 gene) ------- COMMENT: 6e5ece46806fb848 39 cZv/LZWPjqm0GRAJq9Q1fBmcJ0o Figure S1A ------- COMMENT: 6e5ece46806fb848 40 cZv/LZWPjqm0GRAJq9Q1fBmcJ0o Figure S1A ------- COMMENT: 6e5ece46806fb848 41 cZv/LZWPjqm0GRAJq9Q1fBmcJ0o Figure S1A ------- COMMENT: 6e5ece46806fb848 42 cZv/LZWPjqm0GRAJq9Q1fBmcJ0o Figure S1A ------- COMMENT: 6e5ece46806fb848 46 R0ybwCfuPvIoUaX4v9l28IsZc4Y Figure S2 ------- COMMENT: 6e5ece46806fb848 47 R0ybwCfuPvIoUaX4v9l28IsZc4Y Figure S2 ------- COMMENT: 6e5ece46806fb848 48 R0ybwCfuPvIoUaX4v9l28IsZc4Y Figure S2 ------- COMMENT: 6e5ece46806fb848 50 urmGXtTAv31GW4SNZKgE0auOWS8 (comment: mbs1 hotspot quantification ------- COMMENT: 6e5ece46806fb848 54 uP2b450kx3y/u6z3D7bDUa+eEuM fig 8 ------- COMMENT: 6e5ece46806fb848 58 acGVpmK5ESo19l4S0TGlsnyofys Figure S4 ------- COMMENT: 6e5ece46806fb848 59 uP2b450kx3y/u6z3D7bDUa+eEuM fig8 ------- COMMENT: 6e5ece46806fb848 60 jp3qjq8tl9E9blZytyUZqOHskEM fig S4 ------- COMMENT: 6e5ece46806fb848 61 R0ybwCfuPvIoUaX4v9l28IsZc4Y Figure S2 ------- COMMENT: 6e5ece46806fb848 62 ZA2H50HmqkxmmoKkzTBHh5YFE6s Figure S1 ------- COMMENT: 6e7137c59c7c4231 1 sOOlAv/SlkDHhiRxDi1nwRnCAok the Chp1 chromodomain bound both H3K9me2 and H3K9me3 peptides with significantly higher affinity than either Clr4 or Swi6 (Table 1), and all proteins bound H3K9me3 more tightly than H3K9me2. ||we solved the crystal structure of the Chp1 chromodomain (CD) in complex with an H3K9me3 peptide (Figure 1C; Table 2). ------- COMMENT: 6e7137c59c7c4231 2 qfAIhmHtrXvRMRS23tJbpe2wvQM the Chp1 chromodomain bound both H3K9me2 and H3K9me3 peptides with significantly higher affinity than either Clr4 or Swi6 (Table 1), and all proteins bound H3K9me3 more tightly than H3K9me2. || we solved the crystal structure of the Chp1 chromodomain (CD) in complex with an H3K9me3 peptide (Figure 1C; Table 2). ------- COMMENT: 6e7137c59c7c4231 3 qfAIhmHtrXvRMRS23tJbpe2wvQM the Chp1 chromodomain bound both H3K9me2 and H3K9me3 peptides with significantly higher affinity than either Clr4 or Swi6 (Table 1), and all proteins bound H3K9me3 more tightly than H3K9me2. || we solved the crystal structure of the Chp1 chromodomain (CD) in complex with an H3K9me3 peptide (Figure 1C; Table 2). ------- COMMENT: 6e7137c59c7c4231 4 3MPnJlF/6yc725P1TcUJ6z08jM8 the Chp1 chromodomain bound both H3K9me2 and H3K9me3 peptides with significantly higher affinity than either Clr4 or Swi6 (Table 1), and all proteins bound H3K9me3 more tightly than H3K9me2. || we solved the crystal structure of the Chp1 chromodomain (CD) in complex with an H3K9me3 peptide (Figure 1C; Table 2). S10 of histone H3 is phosphorylated during mitosis and displaces HP1 proteins (including Swi6) from chromatin (Fischle et al., 2005; Hirota et al., 2005; Yamada et al., 2005). We investigated whether binding of Swi6 and Chp1 to H3K9me peptides was affected by S10 phosphorylation and found a strong reduction in both Chp1- and Swi6-binding affinity (Table S2; Figure S2B), ------- COMMENT: 6e7137c59c7c4231 5 zoHoQv543tiDn0d/BXWIjR0/2/s the Chp1 chromodomain bound both H3K9me2 and H3K9me3 peptides with significantly higher affinity than either Clr4 or Swi6 (Table 1), and all proteins bound H3K9me3 more tightly than H3K9me2. || we solved the crystal structure of the Chp1 chromodomain (CD) in complex with an H3K9me3 peptide (Figure 1C; Table 2).S10 of histone H3 is phosphorylated during mitosis and displaces HP1 proteins (including Swi6) from chromatin (Fischle et al., 2005; Hirota et al., 2005; Yamada et al., 2005). We investigated whether binding of Swi6 and Chp1 to H3K9me peptides was affected by S10 phosphorylation and found a strong reduction in both Chp1- and Swi6-binding affinity (Table S2; Figure S2B), ------- COMMENT: 6e7137c59c7c4231 6 zoHoQv543tiDn0d/BXWIjR0/2/s the Chp1 chromodomain bound both H3K9me2 and H3K9me3 peptides with significantly higher affinity than either Clr4 or Swi6 (Table 1), and all proteins bound H3K9me3 more tightly than H3K9me2. || we solved the crystal structure of the Chp1 chromodomain (CD) in complex with an H3K9me3 peptide (Figure 1C; Table 2).S10 of histone H3 is phosphorylated during mitosis and displaces HP1 proteins (including Swi6) from chromatin (Fischle et al., 2005; Hirota et al., 2005; Yamada et al., 2005). We investigated whether binding of Swi6 and Chp1 to H3K9me peptides was affected by S10 phosphorylation and found a strong reduction in both Chp1- and Swi6-binding affinity (Table S2; Figure S2B), ------- COMMENT: 6e7137c59c7c4231 7 Mw5dA2uo+hhwbt73AtqVN6geBns and the V24R mutant abolished the specificity of the chromodomain interaction for K9 methylated peptides (Kd > 500 mM). ------- COMMENT: 6e7137c59c7c4231 8 HZyVkRPqjvYWx3/xtzj4O+81sLI A third class showed a more profound reduction in binding affinity: the E23V,V24M mutant reduced binding affinity 40 fold, ------- COMMENT: 6e7137c59c7c4231 9 IPznO6/c59E4f7oDGcwcZ+ItF6g A second class of mutants showed a 5- to 17-fold reduction in binding affinity for H3K9me2 compared with the wild-type Chp1 chromodomain (V21A, E23V, N59A, and V24M). A ------- COMMENT: 6e7137c59c7c4231 10 IPznO6/c59E4f7oDGcwcZ+ItF6g A second class of mutants showed a 5- to 17-fold reduction in binding affinity for H3K9me2 compared with the wild-type Chp1 chromodomain (V21A, E23V, N59A, and V24M). A ------- COMMENT: 6e7137c59c7c4231 11 IPznO6/c59E4f7oDGcwcZ+ItF6g A second class of mutants showed a 5- to 17-fold reduction in binding affinity for H3K9me2 compared with the wild-type Chp1 chromodomain (V21A, E23V, N59A, and V24M). A ------- COMMENT: 6e7137c59c7c4231 12 IPznO6/c59E4f7oDGcwcZ+ItF6g A second class of mutants showed a 5- to 17-fold reduction in binding affinity for H3K9me2 compared with the wild-type Chp1 chromodomain (V21A, E23V, N59A, and V24M). A ------- COMMENT: 6e7137c59c7c4231 13 Anjqu908NkIa79+hyv5McbjAm+I The observed affinities ranged from close to wild-type to complete loss of specific binding. The F61A mutant showed little reduction in binding affinity compared with wild-type Chp1. A ------- COMMENT: 6e7137c59c7c4231 14 eIcDrS6zp7+/LD6uw5q7DmA3Ai8 The Swi6 V82E mutant bound H3K9me2 with 5-fold higher affinity than wild-type Swi6 (Table 1; Figure S2A), ------- COMMENT: 6e7137c59c7c4231 15 WAFx9hD7+7pwU6xp1J2k4i9vYQg Introduction of an E80V mutation, corresponding to V21 of Chp1, into Swi6V82E further increased Swi6’s affinity by 2-fold (Table 1; Figure S2A). ------- COMMENT: 6e7137c59c7c4231 16 lXZK/KXdtmJEtlh+nYBEjNfPum4 Interestingly, cells expressing most of the mutant alleles of chp1 showed no defect in heterochromatin assembly as measured in this assay (Figure 3A), with the exception of the double mutant E23V; V24M and the V24R chp1 mutant, which did show silencing defects. ------- COMMENT: 6e7137c59c7c4231 17 lXZK/KXdtmJEtlh+nYBEjNfPum4 Interestingly, cells expressing most of the mutant alleles of chp1 showed no defect in heterochromatin assembly as measured in this assay (Figure 3A), with the exception of the double mutant E23V; V24M and the V24R chp1 mutant, which did show silencing defects. ------- COMMENT: 6e7137c59c7c4231 18 lXZK/KXdtmJEtlh+nYBEjNfPum4 Interestingly, cells expressing most of the mutant alleles of chp1 showed no defect in heterochromatin assembly as measured in this assay (Figure 3A), with the exception of the double mutant E23V; V24M and the V24R chp1 mutant, which did show silencing defects. ------- COMMENT: 6e7137c59c7c4231 19 lXZK/KXdtmJEtlh+nYBEjNfPum4 Interestingly, cells expressing most of the mutant alleles of chp1 showed no defect in heterochromatin assembly as measured in this assay (Figure 3A), with the exception of the double mutant E23V; V24M and the V24R chp1 mutant, which did show silencing defects. ------- COMMENT: 6e7137c59c7c4231 20 lXZK/KXdtmJEtlh+nYBEjNfPum4 Interestingly, cells expressing most of the mutant alleles of chp1 showed no defect in heterochromatin assembly as measured in this assay (Figure 3A), with the exception of the double mutant E23V; V24M and the V24R chp1 mutant, which did show silencing defects. ------- COMMENT: 6e7137c59c7c4231 21 JO4ijbs6iGS3xmFsWpZEXihcYbY Unlike chp1 null cells, which showed 23% of mitotic cells undergoing chromosome missegregation, the chromodomain point-mutated strains, with the exception of V24Rchp1, showed few cells undergoing aberrant mitoses (Table S3). ------- COMMENT: 6e7137c59c7c4231 22 ZlZ9ubtN/+TDPpcXeKqdGXutoRU While chp1D and chp1CDD cells lack centromeric siRNAs, they were present in all other mutants with the exception of V24R. ------- COMMENT: 6e7137c59c7c4231 23 ZlZ9ubtN/+TDPpcXeKqdGXutoRU While chp1D and chp1CDD cells lack centromeric siRNAs, they were present in all other mutants with the exception of V24R. ------- COMMENT: 6e7137c59c7c4231 24 ZlZ9ubtN/+TDPpcXeKqdGXutoRU While chp1D and chp1CDD cells lack centromeric siRNAs, they were present in all other mutants with the exception of V24R. ------- COMMENT: 6e7137c59c7c4231 25 ZlZ9ubtN/+TDPpcXeKqdGXutoRU While chp1D and chp1CDD cells lack centromeric siRNAs, they were present in all other mutants with the exception of V24R. ------- COMMENT: 6e7137c59c7c4231 26 ZlZ9ubtN/+TDPpcXeKqdGXutoRU While chp1D and chp1CDD cells lack centromeric siRNAs, they were present in all other mutants with the exception of V24R. ------- COMMENT: 6e7137c59c7c4231 27 ZlZ9ubtN/+TDPpcXeKqdGXutoRU While chp1D and chp1CDD cells lack centromeric siRNAs, they were present in all other mutants with the exception of V24R. ------- COMMENT: 6e7137c59c7c4231 28 ZlZ9ubtN/+TDPpcXeKqdGXutoRU While chp1D and chp1CDD cells lack centromeric siRNAs, they were present in all other mutants with the exception of V24R. ------- COMMENT: 6e7137c59c7c4231 29 ZlZ9ubtN/+TDPpcXeKqdGXutoRU While chp1D and chp1CDD cells lack centromeric siRNAs, they were present in all other mutants with the exception of V24R. ------- COMMENT: 6e7137c59c7c4231 30 ZlZ9ubtN/+TDPpcXeKqdGXutoRU While chp1D and chp1CDD cells lack centromeric siRNAs, they were present in all other mutants with the exception of V24R. ------- COMMENT: 6e7137c59c7c4231 31 ZlZ9ubtN/+TDPpcXeKqdGXutoRU While chp1D and chp1CDD cells lack centromeric siRNAs, they were present in all other mutants with the exception of V24R. ------- COMMENT: 6e7137c59c7c4231 32 Ow6CayMBVr5iqmGO91UA5m+rvMM Surprisingly, in contrast to robust association of wild-type Chp1 at the centromeric outer repeat sites, we found only very low levels of many of the mutant Chp1 proteins at centromeres under our standard ChIP conditions (Figure 4A and Figure S5A) ------- COMMENT: 6e7137c59c7c4231 33 Ow6CayMBVr5iqmGO91UA5m+rvMM Surprisingly, in contrast to robust association of wild-type Chp1 at the centromeric outer repeat sites, we found only very low levels of many of the mutant Chp1 proteins at centromeres under our standard ChIP conditions (Figure 4A and Figure S5A) ------- COMMENT: 6e7137c59c7c4231 34 ZlZ9ubtN/+TDPpcXeKqdGXutoRU While chp1D and chp1CDD cells lack centromeric siRNAs, they were present in all other mutants with the exception of V24R. ------- COMMENT: 6e7137c59c7c4231 35 ZlZ9ubtN/+TDPpcXeKqdGXutoRU While chp1D and chp1CDD cells lack centromeric siRNAs, they were present in all other mutants with the exception of V24R. ------- COMMENT: 6e7137c59c7c4231 36 Ow6CayMBVr5iqmGO91UA5m+rvMM Surprisingly, in contrast to robust association of wild-type Chp1 at the centromeric outer repeat sites, we found only very low levels of many of the mutant Chp1 proteins at centromeres under our standard ChIP conditions (Figure 4A and Figure S5A) ------- COMMENT: 6e7137c59c7c4231 37 Ow6CayMBVr5iqmGO91UA5m+rvMM Surprisingly, in contrast to robust association of wild-type Chp1 at the centromeric outer repeat sites, we found only very low levels of many of the mutant Chp1 proteins at centromeres under our standard ChIP conditions (Figure 4A and Figure S5A) ------- COMMENT: 6e7137c59c7c4231 38 Ow6CayMBVr5iqmGO91UA5m+rvMM Surprisingly, in contrast to robust association of wild-type Chp1 at the centromeric outer repeat sites, we found only very low levels of many of the mutant Chp1 proteins at centromeres under our standard ChIP conditions (Figure 4A and Figure S5A) ------- COMMENT: 6e7137c59c7c4231 39 Ow6CayMBVr5iqmGO91UA5m+rvMM Surprisingly, in contrast to robust association of wild-type Chp1 at the centromeric outer repeat sites, we found only very low levels of many of the mutant Chp1 proteins at centromeres under our standard ChIP conditions (Figure 4A and Figure S5A) ------- COMMENT: 6e7137c59c7c4231 40 Ow6CayMBVr5iqmGO91UA5m+rvMM Surprisingly, in contrast to robust association of wild-type Chp1 at the centromeric outer repeat sites, we found only very low levels of many of the mutant Chp1 proteins at centromeres under our standard ChIP conditions (Figure 4A and Figure S5A) ------- COMMENT: 6e7137c59c7c4231 41 Ow6CayMBVr5iqmGO91UA5m+rvMM Surprisingly, in contrast to robust association of wild-type Chp1 at the centromeric outer repeat sites, we found only very low levels of many of the mutant Chp1 proteins at centromeres under our standard ChIP conditions (Figure 4A and Figure S5A) ------- COMMENT: 6e7137c59c7c4231 42 Ow6CayMBVr5iqmGO91UA5m+rvMM Surprisingly, in contrast to robust association of wild-type Chp1 at the centromeric outer repeat sites, we found only very low levels of many of the mutant Chp1 proteins at centromeres under our standard ChIP conditions (Figure 4A and Figure S5A) ------- COMMENT: 6e7137c59c7c4231 43 gVKrbT1NV18exdB/eV554fFOvsg unlike chp1 null cells, there was no significant decrease in centromeric H3K9me2 or Swi6 association in any of the chp1 mutants tested (Figures 4B and 4C; Figure S5B). ------- COMMENT: 6e7137c59c7c4231 44 gVKrbT1NV18exdB/eV554fFOvsg unlike chp1 null cells, there was no significant decrease in centromeric H3K9me2 or Swi6 association in any of the chp1 mutants tested (Figures 4B and 4C; Figure S5B). ------- COMMENT: 6e7137c59c7c4231 45 gVKrbT1NV18exdB/eV554fFOvsg unlike chp1 null cells, there was no significant decrease in centromeric H3K9me2 or Swi6 association in any of the chp1 mutants tested (Figures 4B and 4C; Figure S5B). ------- COMMENT: 6e7137c59c7c4231 46 gVKrbT1NV18exdB/eV554fFOvsg unlike chp1 null cells, there was no significant decrease in centromeric H3K9me2 or Swi6 association in any of the chp1 mutants tested (Figures 4B and 4C; Figure S5B). ------- COMMENT: 6e7137c59c7c4231 47 gVKrbT1NV18exdB/eV554fFOvsg unlike chp1 null cells, there was no significant decrease in centromeric H3K9me2 or Swi6 association in any of the chp1 mutants tested (Figures 4B and 4C; Figure S5B). ------- COMMENT: 6e7137c59c7c4231 48 gVKrbT1NV18exdB/eV554fFOvsg unlike chp1 null cells, there was no significant decrease in centromeric H3K9me2 or Swi6 association in any of the chp1 mutants tested (Figures 4B and 4C; Figure S5B). ------- COMMENT: 6e7137c59c7c4231 49 gVKrbT1NV18exdB/eV554fFOvsg unlike chp1 null cells, there was no significant decrease in centromeric H3K9me2 or Swi6 association in any of the chp1 mutants tested (Figures 4B and 4C; Figure S5B). ------- COMMENT: 6e7137c59c7c4231 50 uI0iEc5jdcF/shnfFk8CBDtryM0 We found, however, that introduction of F276Aago1 into either the E23Vchp1 or V24Mchp1 mutants resulted in loss of silencing of cen::ura4+ . ------- COMMENT: 6e7137c59c7c4231 51 uI0iEc5jdcF/shnfFk8CBDtryM0 We found, however, that introduction of F276Aago1 into either the E23Vchp1 or V24Mchp1 mutants resulted in loss of silencing of cen::ura4+ . ------- COMMENT: 6e7137c59c7c4231 52 jbopQNXSe6J6cQeMQYPbnE94mxk In contrast, the F61Achp1; F276Aago1 mutant strain showed no defect in the silencing of the reporter (Figure 4D). ------- COMMENT: 6e7137c59c7c4231 53 LidpqExX31Zqef0NVJXz8Akq+XU loss of maintenance of heterochromatin was seen in cells expressing both Tas3WG and E23Vchp1, unlike cells expressing either single mutant ------- COMMENT: 6e7137c59c7c4231 54 LidpqExX31Zqef0NVJXz8Akq+XU loss of maintenance of heterochromatin was seen in cells expressing both Tas3WG and E23Vchp1, unlike cells expressing either single mutant ------- COMMENT: 6e7137c59c7c4231 55 NKtK72LzEIKQC3UCfvE42xEPKBc (comment: CHECK ******abolished /de novo**********) Reintegration of clr4+ into cells bearing the chp1 chromodomain mutants showed a striking separation of phenotypes, with some mutants unable to re-establish centromeric heterochromatin (e.g., E23Vchp1clr4D to clr4+, V24Mchp1clr4D to clr4+, and N59Achp1clr4D to clr4+) and others showing efficient re-establishment (e.g., F61Achp1clr4D to clr4+), as assessed by silencing of the cen::ura4+ transgene (Figure 5A). ------- COMMENT: 6e7137c59c7c4231 56 waRCfB8Wxny7MYD8lP9Fl7Q2WwU (comment: CHECK ******abolished /de novo**********) Reintegration of clr4+ into cells bearing the chp1 chromodomain mutants showed a striking separation of phenotypes, with some mutants unable to re-establish centromeric heterochromatin (e.g., E23Vchp1clr4D to clr4+, V24Mchp1clr4D to clr4+, and N59Achp1clr4D to clr4+) and others showing efficient re-establishment (e.g., F61Achp1clr4D to clr4+), as assessed by silencing of the cen::ura4+ transgene (Figure 5A). ------- COMMENT: 6e7137c59c7c4231 57 MXix7OtRLwQii5jlZfkdww7/Sw4 (comment: CHECK ******abolished /de novo**********) Reintegration of clr4+ into cells bearing the chp1 chromodomain mutants showed a striking separation of phenotypes, with some mutants unable to re-establish centromeric heterochromatin (e.g., E23Vchp1clr4D to clr4+, V24Mchp1clr4D to clr4+, and N59Achp1clr4D to clr4+) and others showing efficient re-establishment (e.g., F61Achp1clr4D to clr4+), as assessed by silencing of the cen::ura4+ transgene (Figure 5A). ------- COMMENT: 6e7137c59c7c4231 58 TcO9ycMmYRbt9Z8/H03zqQdrV34 (comment: CHAECK ******abolished /de novo**********) Reintegration of clr4+ into cells bearing the chp1 chromodomain mutants showed a striking separation of phenotypes, with some mutants unable to re-establish centromeric heterochromatin (e.g., E23Vchp1clr4D to clr4+, V24Mchp1clr4D to clr4+, and N59Achp1clr4D to clr4+) and others showing efficient re-establishment (e.g., F61Achp1clr4D to clr4+), as assessed by silencing of the cen::ura4+ transgene (Figure 5A). ------- COMMENT: 6e7137c59c7c4231 59 TcO9ycMmYRbt9Z8/H03zqQdrV34 (comment: CHAECK ******abolished /de novo**********) Reintegration of clr4+ into cells bearing the chp1 chromodomain mutants showed a striking separation of phenotypes, with some mutants unable to re-establish centromeric heterochromatin (e.g., E23Vchp1clr4D to clr4+, V24Mchp1clr4D to clr4+, and N59Achp1clr4D to clr4+) and others showing efficient re-establishment (e.g., F61Achp1clr4D to clr4+), as assessed by silencing of the cen::ura4+ transgene (Figure 5A). ------- COMMENT: 6e7137c59c7c4231 60 4mdVbIO5V0FehrqubzJg1MzKcQU High levels of centromeric transcripts also accumulated in these establishment-defective clr4+ reintroduction strains E23Vchp1clr4D to clr4+, V24Mchp1clr4D to clr4+, and N59Achp1clr4D to clr4+, but not in the establishment-competent F61Achp1clr4D to clr4+ cells (Figure 5B; Figure S9A) ------- COMMENT: 6e7137c59c7c4231 61 4mdVbIO5V0FehrqubzJg1MzKcQU High levels of centromeric transcripts also accumulated in these establishment-defective clr4+ reintroduction strains E23Vchp1clr4D to clr4+, V24Mchp1clr4D to clr4+, and N59Achp1clr4D to clr4+, but not in the establishment-competent F61Achp1clr4D to clr4+ cells (Figure 5B; Figure S9A) ------- COMMENT: 6e7137c59c7c4231 62 4mdVbIO5V0FehrqubzJg1MzKcQU High levels of centromeric transcripts also accumulated in these establishment-defective clr4+ reintroduction strains E23Vchp1clr4D to clr4+, V24Mchp1clr4D to clr4+, and N59Achp1clr4D to clr4+, but not in the establishment-competent F61Achp1clr4D to clr4+ cells (Figure 5B; Figure S9A) ------- COMMENT: 6e7137c59c7c4231 63 sCyAj0GU6TGS3s2E+AFqmkjvWPE The efficiency of chromosome segregation was also monitored in clr4+ reintroduction chp1 mutant cells (Table S4) and closely correlated with Chp1’s binding efficiency. Mutants with>5-fold reduction in H3K9me-binding efficiency that cannot establish centromeric heterochromatin exhibited elevated rates of chromosome missegregation. ------- COMMENT: 6e7137c59c7c4231 64 sCyAj0GU6TGS3s2E+AFqmkjvWPE The efficiency of chromosome segregation was also monitored in clr4+ reintroduction chp1 mutant cells (Table S4) and closely correlated with Chp1’s binding efficiency. Mutants with >5-fold reduction in H3K9me-binding efficiency that cannot establish centromeric heterochromatin exhibited elevated rates of chromosome missegregation. ------- COMMENT: 6e7137c59c7c4231 65 sCyAj0GU6TGS3s2E+AFqmkjvWPE The efficiency of chromosome segregation was also monitored in clr4+ reintroduction chp1 mutant cells (Table S4) and closely correlated with Chp1’s binding efficiency. Mutants with >5-fold reduction in H3K9me-binding efficiency that cannot establish centromeric heterochromatin exhibited elevated rates of chromosome missegregation. ------- COMMENT: 6e7137c59c7c4231 66 sCyAj0GU6TGS3s2E+AFqmkjvWPE The efficiency of chromosome segregation was also monitored in clr4+ reintroduction chp1 mutant cells (Table S4) and closely correlated with Chp1’s binding efficiency. Mutants with >5-fold reduction in H3K9me-binding efficiency that cannot establish centromeric heterochromatin exhibited elevated rates of chromosome missegregation. ------- COMMENT: 6e7137c59c7c4231 67 sCyAj0GU6TGS3s2E+AFqmkjvWPE The efficiency of chromosome segregation was also monitored in clr4+ reintroduction chp1 mutant cells (Table S4) and closely correlated with Chp1’s binding efficiency. Mutants with >5-fold reduction in H3K9me-binding efficiency that cannot establish centromeric heterochromatin exhibited elevated rates of chromosome missegregation. ------- COMMENT: 6e7137c59c7c4231 68 sCyAj0GU6TGS3s2E+AFqmkjvWPE The efficiency of chromosome segregation was also monitored in clr4+ reintroduction chp1 mutant cells (Table S4) and closely correlated with Chp1’s binding efficiency. Mutants with >5-fold reduction in H3K9me-binding efficiency that cannot establish centromeric heterochromatin exhibited elevated rates of chromosome missegregation. ------- COMMENT: 6e7137c59c7c4231 69 QRMWxS355d6uwNrmIs472+UrcHo Surprisingly, when we monitored the presence of centromeric siRNAs in these clr4+ reintroduction strains, we found that even mutants that were defective for establishment of centromeric heterochromatin efficiently synthesized siRNAs derived from both the dg and dh centromeric repeats (Figure 5C) ------- COMMENT: 6e7137c59c7c4231 70 QRMWxS355d6uwNrmIs472+UrcHo Surprisingly, when we monitored the presence of centromeric siRNAs in these clr4+ reintroduction strains, we found that even mutants that were defective for establishment of centromeric heterochromatin efficiently synthesized siRNAs derived from both the dg and dh centromeric repeats (Figure 5C) ------- COMMENT: 6e7137c59c7c4231 71 EFt0kjaKmFrcJlmXqRzFZnmX5IM Results for Chp1 localization were similar to those seen in maintenance strains with mutants such as E23Vchp1clr4D to clr4+ and V24Mchp1clr4D to clr4+ showing little association with centromeres, whereas F61Achp1clr4D to clr4+ was enriched at levels close to wild-type Chp1clr4D to clr4+ ------- COMMENT: 6e7137c59c7c4231 72 EFt0kjaKmFrcJlmXqRzFZnmX5IM Results for Chp1 localization were similar to those seen in maintenance strains with mutants such as E23Vchp1clr4D to clr4+ and V24Mchp1clr4D to clr4+ showing little association with centromeres, whereas F61Achp1clr4D to clr4+ was enriched at levels close to wild-type Chp1clr4D to clr4+ ------- COMMENT: 6e7137c59c7c4231 73 EFt0kjaKmFrcJlmXqRzFZnmX5IM Results for Chp1 localization were similar to those seen in maintenance strains with mutants such as E23Vchp1clr4D to clr4+ and V24Mchp1clr4D to clr4+ showing little association with centromeres, whereas F61Achp1clr4D to clr4+ was enriched at levels close to wild-type Chp1clr4D to clr4+ ------- COMMENT: 6e7137c59c7c4231 74 ieb0Rt6z6+Ww4Se4wQ3ywpMjwsw centromeric H3K9me2 levels were low in many of the chp1 chromodomain clr4+ reintroduction strains and closely mirrored the levels of Chp1 recruitment in the various centromeric H3K9me2 levels were low in many of the chp1 chromodomain clr4+ reintroduction strains and closely mirrored the levels of Chp1 recruitment in the various mutant backgrounds (Figure 6B). ------- COMMENT: 6e7137c59c7c4231 75 ieb0Rt6z6+Ww4Se4wQ3ywpMjwsw centromeric H3K9me2 levels were low in many of the chp1 chromodomain clr4+ reintroduction strains and closely mirrored the levels of Chp1 recruitment in the various centromeric H3K9me2 levels were low in many of the chp1 chromodomain clr4+ reintroduction strains and closely mirrored the levels of Chp1 recruitment in the various mutant backgrounds (Figure 6B). ------- COMMENT: 6e7137c59c7c4231 76 ieb0Rt6z6+Ww4Se4wQ3ywpMjwsw centromeric H3K9me2 levels were low in many of the chp1 chromodomain clr4+ reintroduction strains and closely mirrored the levels of Chp1 recruitment in the various centromeric H3K9me2 levels were low in many of the chp1 chromodomain clr4+ reintroduction strains and closely mirrored the levels of Chp1 recruitment in the various mutant backgrounds (Figure 6B). ------- COMMENT: 6e7137c59c7c4231 77 ieb0Rt6z6+Ww4Se4wQ3ywpMjwsw centromeric H3K9me2 levels were low in many of the chp1 chromodomain clr4+ reintroduction strains and closely mirrored the levels of Chp1 recruitment in the various centromeric H3K9me2 levels were low in many of the chp1 chromodomain clr4+ reintroduction strains and closely mirrored the levels of Chp1 recruitment in the various mutant backgrounds (Figure 6B). ------- COMMENT: 6e7137c59c7c4231 78 ibk1E3izRwJTtvgIWBVW+uiZMAA High copy expression of clr4+ in the E23Vchp1clr4D and in the V24Mchp1clr4D cells allowed efficient establishment of centromeric heterochromatin (Figure 6C). Thus, the establishment defect of chp1 mutants with reduced H3K9me-binding affinity can be compensated by an increased dosage of clr4+ . ------- COMMENT: 6e7137c59c7c4231 79 ibk1E3izRwJTtvgIWBVW+uiZMAA High copy expression of clr4+ in the E23Vchp1clr4D and in the V24Mchp1clr4D cells allowed efficient establishment of centromeric heterochromatin (Figure 6C). Thus, the establishment defect of chp1 mutants with reduced H3K9me-binding affinity can be compensated by an increased dosage of clr4+ . ------- COMMENT: 6e7137c59c7c4231 80 3MPnJlF/6yc725P1TcUJ6z08jM8 the Chp1 chromodomain bound both H3K9me2 and H3K9me3 peptides with significantly higher affinity than either Clr4 or Swi6 (Table 1), and all proteins bound H3K9me3 more tightly than H3K9me2. || we solved the crystal structure of the Chp1 chromodomain (CD) in complex with an H3K9me3 peptide (Figure 1C; Table 2). S10 of histone H3 is phosphorylated during mitosis and displaces HP1 proteins (including Swi6) from chromatin (Fischle et al., 2005; Hirota et al., 2005; Yamada et al., 2005). We investigated whether binding of Swi6 and Chp1 to H3K9me peptides was affected by S10 phosphorylation and found a strong reduction in both Chp1- and Swi6-binding affinity (Table S2; Figure S2B), ------- COMMENT: 6e7137c59c7c4231 81 zoHoQv543tiDn0d/BXWIjR0/2/s the Chp1 chromodomain bound both H3K9me2 and H3K9me3 peptides with significantly higher affinity than either Clr4 or Swi6 (Table 1), and all proteins bound H3K9me3 more tightly than H3K9me2. || we solved the crystal structure of the Chp1 chromodomain (CD) in complex with an H3K9me3 peptide (Figure 1C; Table 2).S10 of histone H3 is phosphorylated during mitosis and displaces HP1 proteins (including Swi6) from chromatin (Fischle et al., 2005; Hirota et al., 2005; Yamada et al., 2005). We investigated whether binding of Swi6 and Chp1 to H3K9me peptides was affected by S10 phosphorylation and found a strong reduction in both Chp1- and Swi6-binding affinity (Table S2; Figure S2B), ------- COMMENT: 6e7137c59c7c4231 82 zoHoQv543tiDn0d/BXWIjR0/2/s the Chp1 chromodomain bound both H3K9me2 and H3K9me3 peptides with significantly higher affinity than either Clr4 or Swi6 (Table 1), and all proteins bound H3K9me3 more tightly than H3K9me2. || we solved the crystal structure of the Chp1 chromodomain (CD) in complex with an H3K9me3 peptide (Figure 1C; Table 2).S10 of histone H3 is phosphorylated during mitosis and displaces HP1 proteins (including Swi6) from chromatin (Fischle et al., 2005; Hirota et al., 2005; Yamada et al., 2005). We investigated whether binding of Swi6 and Chp1 to H3K9me peptides was affected by S10 phosphorylation and found a strong reduction in both Chp1- and Swi6-binding affinity (Table S2; Figure S2B), ------- COMMENT: 6e84b9c4c93de258 2 NGVx0xLnYF5mLnERcqhR0waCwjc Figure 1, A and B All of these required passage through G1 (second mitosis) ------- COMMENT: 6e84b9c4c93de258 4 jeuxtksFysT9VRz4w0lVK8E/i/o Figure 1F & 7B (second mitosis) ------- COMMENT: 6e84b9c4c93de258 8 PxGYNeSxrV5PD+XotSQQNBxfnSo fig 1a ------- COMMENT: 6e84b9c4c93de258 17 YnI3sHg7ttDSlmqEiY2AgChnjTc figure3 ------- COMMENT: 6e84b9c4c93de258 20 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 6e84b9c4c93de258 24 asaP9qj2LqfTkEGT3ytMhpkNnAU figue 4 ------- COMMENT: 6e84b9c4c93de258 27 JkuhWFsmVfTfGpFB05nadNgyT4k These results showed that Mis12 was localized at centromeres throughout the cell cycle ------- COMMENT: 6e84b9c4c93de258 33 qZ03F2KTka3Ibes5t6oWQTPtpOQ (Fig. 6D): Mis12 is thus required for maintaining the inner centromere structure. ------- COMMENT: 6e84b9c4c93de258 37 NGVx0xLnYF5mLnERcqhR0waCwjc Figure 1, A and B All of these required passage through G1 (second mitosis) ------- COMMENT: 6e84b9c4c93de258 38 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 6e84b9c4c93de258 39 XVqC+jnxTIe8Ay+zDZV35y7bHp0 figure 3 (comment: before phase 3 extension) ------- COMMENT: 6e84b9c4c93de258 40 6Xon/1mGcjkBNojPWzDNT/QoPJ0 figure 6 Hence, mis6–HA could interact with the centromere in the absence of functional Mis12. ------- COMMENT: 6e84b9c4c93de258 41 PlacBpkdPi9tVQZdg5YknFgmk1o figure 6 Conversely, the mis6-302 strain integrated with the Mis12–HA gene was used. ------- COMMENT: 6e84b9c4c93de258 42 mCO5iz18oDQQfDMWgLhu65RcYfk suggesting that sister centromeres were separated in the metaphase-arrested cells. ------- COMMENT: 6e84b9c4c93de258 43 TdI9+f5nKDENfchGFxxKCcyaRDg figure 8a ------- COMMENT: 6e84b9c4c93de258 44 TdI9+f5nKDENfchGFxxKCcyaRDg figure 8a ------- COMMENT: 6e84b9c4c93de258 45 TdI9+f5nKDENfchGFxxKCcyaRDg figure 8a ------- COMMENT: 6e84b9c4c93de258 46 CCPEa9a1WkVUsR7CIFP2L4jykmA (comment: CHECK dis1-delta (cs) viability is rescued by mis12 mutant) ------- COMMENT: 6e84b9c4c93de258 47 lJV3h7JL4NPWFKNycczzQmd9ExM (comment: CHECK dis1-delta (cs) is usually viable at 33) ------- COMMENT: 6e89c33ec1a6bb2d 2 ad2R+yUdH3RglR2OS18pD2NmefE (Figure 1) ------- COMMENT: 6e89c33ec1a6bb2d 3 ad2R+yUdH3RglR2OS18pD2NmefE (Figure 1) ------- COMMENT: 6e89c33ec1a6bb2d 4 ad2R+yUdH3RglR2OS18pD2NmefE (Figure 1) ------- COMMENT: 6e89c33ec1a6bb2d 5 ad2R+yUdH3RglR2OS18pD2NmefE (Figure 1) ------- COMMENT: 6e89c33ec1a6bb2d 6 lpq/bp1uc8Qhf3FWWrXV+GmfMNo (Figure 2) ------- COMMENT: 6e89c33ec1a6bb2d 7 lpq/bp1uc8Qhf3FWWrXV+GmfMNo (Figure 2) ------- COMMENT: 6e89c33ec1a6bb2d 8 lpq/bp1uc8Qhf3FWWrXV+GmfMNo (Figure 2) ------- COMMENT: 6e89c33ec1a6bb2d 9 lpq/bp1uc8Qhf3FWWrXV+GmfMNo (Figure 2) ------- COMMENT: 6e89c33ec1a6bb2d 10 lpq/bp1uc8Qhf3FWWrXV+GmfMNo (Figure 2) ------- COMMENT: 6e89c33ec1a6bb2d 13 lpq/bp1uc8Qhf3FWWrXV+GmfMNo (Figure 2) ------- COMMENT: 6e89c33ec1a6bb2d 14 lpq/bp1uc8Qhf3FWWrXV+GmfMNo (Figure 2) ------- COMMENT: 6e89c33ec1a6bb2d 15 lpq/bp1uc8Qhf3FWWrXV+GmfMNo (Figure 2) ------- COMMENT: 6e89c33ec1a6bb2d 16 lpq/bp1uc8Qhf3FWWrXV+GmfMNo (Figure 2) ------- COMMENT: 6e89c33ec1a6bb2d 17 KexZJLcBsx+PTMTb52RZYuVDR5U (Figure 4) ------- COMMENT: 6e89c33ec1a6bb2d 18 KexZJLcBsx+PTMTb52RZYuVDR5U (Figure 4) ------- COMMENT: 6e89c33ec1a6bb2d 19 KexZJLcBsx+PTMTb52RZYuVDR5U (Figure 4) ------- COMMENT: 6e89c33ec1a6bb2d 21 KexZJLcBsx+PTMTb52RZYuVDR5U (Figure 4) ------- COMMENT: 6e89c33ec1a6bb2d 22 KexZJLcBsx+PTMTb52RZYuVDR5U (Figure 4) ------- COMMENT: 6e89c33ec1a6bb2d 23 KexZJLcBsx+PTMTb52RZYuVDR5U (Figure 4) ------- COMMENT: 6e89c33ec1a6bb2d 24 KexZJLcBsx+PTMTb52RZYuVDR5U (Figure 4) ------- COMMENT: 6e89c33ec1a6bb2d 25 KexZJLcBsx+PTMTb52RZYuVDR5U (Figure 4) ------- COMMENT: 6e89c33ec1a6bb2d 26 KexZJLcBsx+PTMTb52RZYuVDR5U (Figure 4) ------- COMMENT: 6e89c33ec1a6bb2d 27 KexZJLcBsx+PTMTb52RZYuVDR5U (Figure 4) ------- COMMENT: 6e89c33ec1a6bb2d 28 KexZJLcBsx+PTMTb52RZYuVDR5U (Figure 4) ------- COMMENT: 6e89c33ec1a6bb2d 29 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 30 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 31 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 32 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 33 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 34 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 35 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 36 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 37 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 38 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 39 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 40 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 41 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 42 0yR2ShxM14tkgqmGgwG6Tx+C4/Y (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 44 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 45 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 46 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 47 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 48 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 49 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 50 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 51 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 52 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 53 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 54 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 55 z8Gq+5yZWzElpMNz1TTOuCaYrEE (Figure 5) ------- COMMENT: 6e89c33ec1a6bb2d 56 lpq/bp1uc8Qhf3FWWrXV+GmfMNo (Figure 2) ------- COMMENT: 6e89c33ec1a6bb2d 57 lpq/bp1uc8Qhf3FWWrXV+GmfMNo (Figure 2) ------- COMMENT: 6e89c33ec1a6bb2d 59 o0WcM27MjKxQKAST1Jc4QuWNqlM ev4 ------- COMMENT: 6e89c33ec1a6bb2d 60 o0WcM27MjKxQKAST1Jc4QuWNqlM ev4 ------- COMMENT: 6e89c33ec1a6bb2d 61 ecG9iJYKwZlm374BpDtd4C/XAzA (Figure 7) ------- COMMENT: 6e89c33ec1a6bb2d 62 ecG9iJYKwZlm374BpDtd4C/XAzA (Figure 7) ------- COMMENT: 6e89c33ec1a6bb2d 63 ecG9iJYKwZlm374BpDtd4C/XAzA (Figure 7) ------- COMMENT: 6e89c33ec1a6bb2d 64 ecG9iJYKwZlm374BpDtd4C/XAzA (Figure 7) ------- COMMENT: 6e89c33ec1a6bb2d 65 ecG9iJYKwZlm374BpDtd4C/XAzA (Figure 7) ------- COMMENT: 6e89c33ec1a6bb2d 66 ecG9iJYKwZlm374BpDtd4C/XAzA (Figure 7) ------- COMMENT: 6e89c33ec1a6bb2d 67 ecG9iJYKwZlm374BpDtd4C/XAzA (Figure 7) ------- COMMENT: 6e89c33ec1a6bb2d 68 ecG9iJYKwZlm374BpDtd4C/XAzA (Figure 7) ------- COMMENT: 6e89c33ec1a6bb2d 69 ecG9iJYKwZlm374BpDtd4C/XAzA (Figure 7) ------- COMMENT: 6e89c33ec1a6bb2d 70 ecG9iJYKwZlm374BpDtd4C/XAzA (Figure 7) ------- COMMENT: 6e89c33ec1a6bb2d 71 ecG9iJYKwZlm374BpDtd4C/XAzA (Figure 7) ------- COMMENT: 6e89c33ec1a6bb2d 72 ecG9iJYKwZlm374BpDtd4C/XAzA (Figure 7) ------- COMMENT: 6e89c33ec1a6bb2d 73 ecG9iJYKwZlm374BpDtd4C/XAzA (Figure 7) ------- COMMENT: 6e89c33ec1a6bb2d 74 ecG9iJYKwZlm374BpDtd4C/XAzA (Figure 7) ------- COMMENT: 6e89c33ec1a6bb2d 75 ecG9iJYKwZlm374BpDtd4C/XAzA (Figure 7) ------- COMMENT: 6e89c33ec1a6bb2d 76 ecG9iJYKwZlm374BpDtd4C/XAzA (Figure 7) ------- COMMENT: 6e89c33ec1a6bb2d 77 ecG9iJYKwZlm374BpDtd4C/XAzA (Figure 7) ------- COMMENT: 6e89c33ec1a6bb2d 78 dZ64kJEfIQ86jDaQwhjzstaoVn4 ev4,ef ------- COMMENT: 6e903e61b25296eb 1 c+wFOAsjXJODqPGHOQ+OXharw60 FIG. 1. Zhf is required for growth on high and low zinc ------- COMMENT: 6e903e61b25296eb 2 c+wFOAsjXJODqPGHOQ+OXharw60 FIG. 1. Zhf is required for growth on high and low zinc ------- COMMENT: 6e903e61b25296eb 3 CxNIMIM1v57+31u3xweNrKLjD/A Most importantly, the zhf strain was hypersensitive to zinc showing impaired growth on rich medium (YE5S) compared with the equivalent control strain and was unable to grow in medium supplemented with 20 M ZnSO4 (Fig. 1C). ------- COMMENT: 6e903e61b25296eb 4 OArcQ6IwT9JmpLrRJ1h94XVxOBM Zhf Is Required for Growth on Low Zinc ------- COMMENT: 6e903e61b25296eb 5 Fg0vojhj2jr7v+xmLlBysRM1qUY Zinc caused the accumulation of zym1 transcripts with up to 10 –20-fold increase 30 min after the addition of ZnSO4 (Fig. 3, A and C). ------- COMMENT: 6e903e61b25296eb 6 poz4i9cxYysJhgUpxFP1Ck251hM Both the basal and zinc-induced levels of zym1 transcripts were severely reduced in wis1 (Fig. 3C). ------- COMMENT: 6e903e61b25296eb 7 GVhRLBxZLd6gN0UYDomumDphwJw but this pathway is not obligatory for zinc perception because zinc induction was retained in Wis1 mutants albeit at reduced magnitude. ------- COMMENT: 6e903e61b25296eb 8 KW/4k01bP4usR43eoCoOzTn3qws The abundance of zym1 transcripts was also re- duced in cells lacking Pcr1, a bZIP transcription factor that in conjunction with Atf1 functions downstream of Sty1 (41, 42) (Fig. 3C). ------- COMMENT: 6e903e61b25296eb 11 Tw6qwLvjfpaj9Xczf4lENsNUFzo The growth of the resulting zym1 showed only a small but reproducible impairment in rich me- dium (YE5S) supplemented with 10 –100 M zinc (Fig. 5A). ------- COMMENT: 6e903e61b25296eb 12 EDkaGvE0lR2bn3bVjULShGLyeMo Cadmium increased the proportion of longer zym1 transcripts as observed previously following the exposure to copper (Fig. 6B). ------- COMMENT: 6ed5c1503e2a7ae8 1 hp8CQMHiJwpzdInF8Zhen96N3Wg (comment: Binding site: 200-307a.a.) ------- COMMENT: 6ed5c1503e2a7ae8 3 yCFEZaWGJ+JNZypXgLYarcaGC6Q (comment: DNA binding site: 1-60 a.a.) ------- COMMENT: 6ed5c1503e2a7ae8 4 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 6ed5c1503e2a7ae8 5 dHLvVMp6Ijyx6xzq1CDzWUAd4gs Deletion of LEM domain decreases the association of Lem2 at the centromere ------- COMMENT: 6ed5c1503e2a7ae8 6 lQZGB5rmWiDphVocVhC5EStIhBw fig 1a (comment: residues 200-307) ------- COMMENT: 6ed5c1503e2a7ae8 7 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 6ed5c1503e2a7ae8 8 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 6ed5c1503e2a7ae8 9 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 6ed5c1503e2a7ae8 10 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 6ed5c1503e2a7ae8 11 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 6ed5c1503e2a7ae8 13 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 6ed5c1503e2a7ae8 14 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 6ed5c1503e2a7ae8 15 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 6ed5c1503e2a7ae8 16 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 6ed5c1503e2a7ae8 17 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 6ed5c1503e2a7ae8 18 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 6ed5c1503e2a7ae8 19 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 6ed5c1503e2a7ae8 20 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 6ed5c1503e2a7ae8 21 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 6ed5c1503e2a7ae8 22 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 6ed5c1503e2a7ae8 23 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 6ed5c1503e2a7ae8 26 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 6edf172f7a86963f 1 cE7ByCxBVEwc5r/XYlEwfX+Pd3I Exo2-GFP localizes to stress granules ------- COMMENT: 6edf172f7a86963f 2 HDRX4Z36lbWOjINiI/Us84wvR3o SPAC12G12.09-mCherry localizes to stress granules ------- COMMENT: 6f2bb502b0b09c2b 2 vu4LWuoK4bm1ZM2RmA/WZiA8OFo (comment: CHECK tRNA) ------- COMMENT: 6f2c464590b4ee1b 1 OjkSF4eQvWXTvSjGgGe5Pf5ItWE (comment: ubiquitin conjugate) ------- COMMENT: 6f2c464590b4ee1b 2 OjkSF4eQvWXTvSjGgGe5Pf5ItWE (comment: ubiquitin conjugate) ------- COMMENT: 6f44322282953a7c 1 oAQJxN0m+ULu4EefQZ660auP/ug fig 2C ------- COMMENT: 6f44322282953a7c 2 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: 6f48f05c223d7948 2 Er+JRCRz6pA1lzwVyPMjNKjmH9s figure 2d ------- COMMENT: 6f48f05c223d7948 3 Er+JRCRz6pA1lzwVyPMjNKjmH9s figure 2d ------- COMMENT: 6f48f05c223d7948 4 vyqzvqKaHQFDEnwKyOWox8+eZBM figure 2e ------- COMMENT: 6f48f05c223d7948 5 vyqzvqKaHQFDEnwKyOWox8+eZBM figure 2e ------- COMMENT: 6f48f05c223d7948 6 vyqzvqKaHQFDEnwKyOWox8+eZBM figure 2e ------- COMMENT: 6f48f05c223d7948 7 vyqzvqKaHQFDEnwKyOWox8+eZBM figure 2e ------- COMMENT: 6f48f05c223d7948 8 vyqzvqKaHQFDEnwKyOWox8+eZBM figure 2e ------- COMMENT: 6f48f05c223d7948 9 h3o7SOr8wdlQlvGfpVCNIZVstfY figure 2f ------- COMMENT: 6f48f05c223d7948 10 h3o7SOr8wdlQlvGfpVCNIZVstfY figure 2f ------- COMMENT: 6f48f05c223d7948 11 h3o7SOr8wdlQlvGfpVCNIZVstfY figure 2f ------- COMMENT: 6f48f05c223d7948 12 wHWja8QHJNpQ8VpcQ6wkAKwvdqk figure 2g ------- COMMENT: 6f48f05c223d7948 13 V5KMiVezUnja/RciehCCfRfe1y0 Figure 3b ------- COMMENT: 6f48f05c223d7948 14 V5KMiVezUnja/RciehCCfRfe1y0 Figure 3b ------- COMMENT: 6f48f05c223d7948 15 FnI8kzviS6kGF4O7mHuCSZyPdg4 Figure 3c ------- COMMENT: 6f48f05c223d7948 16 FnI8kzviS6kGF4O7mHuCSZyPdg4 Figure 3c ------- COMMENT: 6f48f05c223d7948 19 NjPx0oyNCLmXXf+Ikj3kTqyGIEU Figure 3d ------- COMMENT: 6f48f05c223d7948 20 NjPx0oyNCLmXXf+Ikj3kTqyGIEU Figure 3d ------- COMMENT: 6f7cf08e7e6712ec 1 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 6f7cf08e7e6712ec 2 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 6fa5a621a1388c10 6 CGrj3zms0oyjSnhqqNtdU9QCtdQ inviable mononucleate aseptate vegetative cell with cell cycle arrest in mitotic G2 phase ------- COMMENT: 6fc9b53e91be9283 25 oxF+crVEqbiHO4p52B4h6Vz3dFw (comment: CHECK Background Atf1.10D) ------- COMMENT: 6fc9b53e91be9283 58 IF1zxRv4IWY17Sb4gndq6MBvoCk (comment: same as mas5delta alone) ------- COMMENT: 6fc9b53e91be9283 59 IF1zxRv4IWY17Sb4gndq6MBvoCk (comment: same as mas5delta alone) ------- COMMENT: 6ffc63e387ccfe13 1 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 6ffc63e387ccfe13 2 4rFzUSjNIANFax7rUho3km4AlJI (Fig. 1A, B and C and Fig. 5) ------- COMMENT: 6ffc63e387ccfe13 3 77BX97Fy/WpQshPpHe6LX4ohkq4 (Fig. 1A and B) ------- COMMENT: 6ffc63e387ccfe13 4 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 6ffc63e387ccfe13 5 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 6ffc63e387ccfe13 6 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 6ffc63e387ccfe13 7 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 6ffc63e387ccfe13 8 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: 6ffc63e387ccfe13 9 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 6ffc63e387ccfe13 10 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 6ffc63e387ccfe13 11 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 6ffc63e387ccfe13 12 p3x2dDTjQlS9jbO+IMHpHbED4nw (Fig. 1D) ------- COMMENT: 6ffc63e387ccfe13 13 p3x2dDTjQlS9jbO+IMHpHbED4nw (Fig. 1D) ------- COMMENT: 6ffc63e387ccfe13 14 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: 6ffc63e387ccfe13 15 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: 6ffc63e387ccfe13 16 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: 6ffc63e387ccfe13 17 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: 6ffc63e387ccfe13 18 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: 6ffc63e387ccfe13 19 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: 6ffc63e387ccfe13 20 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: 6ffc63e387ccfe13 21 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: 6ffc63e387ccfe13 22 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: 6ffc63e387ccfe13 23 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: 6ffc63e387ccfe13 24 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A,B) ------- COMMENT: 6ffc63e387ccfe13 25 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: 6ffc63e387ccfe13 26 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: 6ffc63e387ccfe13 27 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: 6ffc63e387ccfe13 28 JGUAK0hR0qRb2BJ50/d3pqvhqJ0 (Fig. 1A,3A) ------- COMMENT: 6ffc63e387ccfe13 29 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 6ffc63e387ccfe13 30 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: 6ffc63e387ccfe13 31 JGUAK0hR0qRb2BJ50/d3pqvhqJ0 (Fig. 1A,3A) ------- COMMENT: 6ffc63e387ccfe13 32 JGUAK0hR0qRb2BJ50/d3pqvhqJ0 (Fig. 1A,3A) ------- COMMENT: 6ffc63e387ccfe13 33 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: 6ffc63e387ccfe13 34 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: 6ffc63e387ccfe13 35 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 6ffc63e387ccfe13 36 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 6ffc63e387ccfe13 37 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 6ffc63e387ccfe13 38 lzDpBhI+e1KR8hAG7s00IgdEh2I (Fig. 5A) ------- COMMENT: 6ffc63e387ccfe13 39 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: 6ffc63e387ccfe13 40 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: 6ffc63e387ccfe13 41 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: 6ffc63e387ccfe13 42 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: 6ffc63e387ccfe13 43 DVJMM6AvJyLz8J8dkwtY+pFizxc (Fig. 5E) ------- COMMENT: 6ffc63e387ccfe13 44 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: 6ffc63e387ccfe13 45 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: 6ffc63e387ccfe13 46 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: 6ffc63e387ccfe13 47 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: 6ffc63e387ccfe13 48 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: 6ffc63e387ccfe13 49 lzDpBhI+e1KR8hAG7s00IgdEh2I (Fig. 5A) ------- COMMENT: 6ffc63e387ccfe13 50 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: 6ffc63e387ccfe13 51 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: 6ffc63e387ccfe13 52 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: 6ffc63e387ccfe13 53 lzDpBhI+e1KR8hAG7s00IgdEh2I (Fig. 5A) ------- COMMENT: 6ffc63e387ccfe13 54 lzDpBhI+e1KR8hAG7s00IgdEh2I (Fig. 5A) ------- COMMENT: 6ffc63e387ccfe13 55 DVJMM6AvJyLz8J8dkwtY+pFizxc (Fig. 5E) ------- COMMENT: 6ffedac206257aa0 8 5GMubp1LHAOzITjde9LMzsg8LDo proteasomal ------- COMMENT: 705d6edbff5f2b70 73 c0k+YozmTuKOUBmwUAR3hn/X2xM Finally, we examined whether Lem2 affected their subcellular localization. GFP-Cho2, GFP-Ole1 and Erg11-GFP were localized in the cortical ER and NE (or perinuclear ER), and deletion of the lem2+ gene did not affect the localization (Fig. 2B), indicating that Lem2 is not necessary for their NE localization ------- COMMENT: 705d6edbff5f2b70 74 c0k+YozmTuKOUBmwUAR3hn/X2xM Finally, we examined whether Lem2 affected their subcellular localization. GFP-Cho2, GFP-Ole1 and Erg11-GFP were localized in the cortical ER and NE (or perinuclear ER), and deletion of the lem2+ gene did not affect the localization (Fig. 2B), indicating that Lem2 is not necessary for their NE localization ------- COMMENT: 705d6edbff5f2b70 75 c0k+YozmTuKOUBmwUAR3hn/X2xM Finally, we examined whether Lem2 affected their subcellular localization. GFP-Cho2, GFP-Ole1 and Erg11-GFP were localized in the cortical ER and NE (or perinuclear ER), and deletion of the lem2+ gene did not affect the localization (Fig. 2B), indicating that Lem2 is not necessary for their NE localization ------- COMMENT: 705d6edbff5f2b70 82 mHdtzeTcAFdxcj8kzeTygLBubtQ We performed an IP–WB experiment on the bqt4 background (‘bqt4’ in Fig.2A). Deletion of bqt4+ did not affect these interactions, indicating that Cho2, Ole1 and Erg11 interact with Lem2 independent of Bqt4. ------- COMMENT: 705d6edbff5f2b70 83 mHdtzeTcAFdxcj8kzeTygLBubtQ We performed an IP–WB experiment on the bqt4 background (‘bqt4’ in Fig.2A). Deletion of bqt4+ did not affect these interactions, indicating that Cho2, Ole1 and Erg11 interact with Lem2 independent of Bqt4. ------- COMMENT: 705d6edbff5f2b70 84 mHdtzeTcAFdxcj8kzeTygLBubtQ We performed an IP–WB experiment on the bqt4 background (‘bqt4’ in Fig.2A). Deletion of bqt4+ did not affect these interactions, indicating that Cho2, Ole1 and Erg11 interact with Lem2 independent of Bqt4. ------- COMMENT: 705d6edbff5f2b70 85 uKtqxUrhRaQ0cXZDQhpGMeBfick Fig.3D ------- COMMENT: 70672a5b4182aefe 4 00CUlcYgBdrvP4xa2MBSbm+6zh8 (comment: CHECK cdc15 localization to actin cortical patch) ------- COMMENT: 708918cfe8faf590 1 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: 708918cfe8faf590 2 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: 708918cfe8faf590 4 Sgz7vXnf2J5Z7p1T/Ap1WwZ9cMA DNS ------- COMMENT: 708918cfe8faf590 6 L9FLH8aW2kJ8lvcBQY6PfeYC00A (Figure 1) ------- COMMENT: 708918cfe8faf590 7 pcVgnw1nOLLLkoYu3XgHvG/vCRY (Figure 1) after 8 hours, medial region of the cells continued to accumulate excess cell wall material ------- COMMENT: 708918cfe8faf590 8 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 708918cfe8faf590 9 z2Fv8/DvN7hbBbqC3NvMdXSUHZg (DNS) In all tetrads, the viable colonies were Arp21 Ura2, indicating that arp21 is an essential gene ------- COMMENT: 708918cfe8faf590 10 D2rx4yAZrjf5h4M+WfV0CazQj0U (Fig 3) ------- COMMENT: 708918cfe8faf590 11 D2rx4yAZrjf5h4M+WfV0CazQj0U (Fig 3) ------- COMMENT: 708918cfe8faf590 12 bGa9JBt8xbo48S6rdGBDD7l2DrE (Fig 3) (comment: CHECK synthetic rescue of cdc3) ------- COMMENT: 708918cfe8faf590 13 KexZJLcBsx+PTMTb52RZYuVDR5U (Figure 4) ------- COMMENT: 708918cfe8faf590 14 KexZJLcBsx+PTMTb52RZYuVDR5U (Figure 4) ------- COMMENT: 708918cfe8faf590 15 KexZJLcBsx+PTMTb52RZYuVDR5U (Figure 4) ------- COMMENT: 708918cfe8faf590 16 XTCZUJAAP/OV9IA4v7isdmdbLCM In arp2-1 mutant cells grown at the restrictive temperature, the protein was not detected in patches. Rather, it appeared to be diffusely distributed throughout the cytoplasm (Figure 4C). ------- COMMENT: 708918cfe8faf590 17 iYDrQg8KLFw2GQzk5xYyeRfmDbA (Fig 6) ------- COMMENT: 708918cfe8faf590 18 iYDrQg8KLFw2GQzk5xYyeRfmDbA (Fig 6) ------- COMMENT: 708918cfe8faf590 19 iYDrQg8KLFw2GQzk5xYyeRfmDbA (Fig 6) ------- COMMENT: 708918cfe8faf590 20 3RrNbqeHzMEBfXx/hmmUnKq6CPo suggesting that the mutant protein is likely less stable than the wild-type Arp2 protein (Figure 6B), a hypothesis confirmed below (see Figure 8C). ------- COMMENT: 708918cfe8faf590 21 iYDrQg8KLFw2GQzk5xYyeRfmDbA (Fig 6) ------- COMMENT: 708918cfe8faf590 22 iYDrQg8KLFw2GQzk5xYyeRfmDbA (Fig 6) ------- COMMENT: 708918cfe8faf590 23 AC/92QxfNf9KjwT5Rn6whpm5X4E (Figure 6) ------- COMMENT: 708918cfe8faf590 24 3ZQ14VSnxZvdcgPVIoibiam7cxQ (Figure 6D) ------- COMMENT: 708918cfe8faf590 25 B4XHTH6J/T4HYGfcvpfE9/8tT8w Fig 8 A After UV irradiation, we found that both wild-type Arp2p and Arp3p were labeled by 8-azido-[a-32P]ATP, indicating that these actin-related proteins bind ATP as predicted ------- COMMENT: 708918cfe8faf590 26 B4XHTH6J/T4HYGfcvpfE9/8tT8w Fig 8 A After UV irradiation, we found that both wild-type Arp2p and Arp3p were labeled by 8-azido-[a-32P]ATP, indicating that these actin-related proteins bind ATP as predicted ------- COMMENT: 708918cfe8faf590 27 r2uUr3Zh9dHQLVYYBN7C1aOwkFU (Figure 8A). T12A protein was also labeled by the ATP analogue, but to a much lesser degree than wild-type Arp2p ------- COMMENT: 708918cfe8faf590 28 N0leq1BZU77pBzawREXp6y3yUr0 (Figure 8A). Arp2-E316K mutant protein had a reduced affinity for ATP compared with wild type Arp2p ------- COMMENT: 708918cfe8faf590 29 RiChWsEhQwSqS0Gl9brs2PAG16M (Figure 8B & C) These results clearly establish that the mutant Arp2-E316K protein turns over more rapidly than wild-type Arp2 protein ------- COMMENT: 708918cfe8faf590 30 +w95GdYS0tEM26BIcHjbq1rHiU0 (Figure 8) The most striking difference between wild-type Arp2p and Arp2-E316K was the failure to coimmunoprecipitate labeled Arp3p with Arp2-E316K ------- COMMENT: 7097a362b55929af 1 YYgzGAUr7wJgZmsQ2/iXYW6vcCI (comment: CHECK during premeiotic DNA replication) ------- COMMENT: 7097a362b55929af 2 YYgzGAUr7wJgZmsQ2/iXYW6vcCI (comment: CHECK during premeiotic DNA replication) ------- COMMENT: 7097a362b55929af 4 YYgzGAUr7wJgZmsQ2/iXYW6vcCI (comment: CHECK during premeiotic DNA replication) ------- COMMENT: 709eb266115f50d4 1 DCLtWtoUdb2ehcbL+nmH3js6ow8 Fig 1 (comment: vw interpretation for "active form") ------- COMMENT: 709eb266115f50d4 4 Rfx5nkFRaHAfV7gMRA6c+/2DuO0 Fig2A loss of cdc16 function does not affect cdc7 kinase activity ------- COMMENT: 709eb266115f50d4 5 56BCvUXBV0ZDpZuRpd+6xbpE8zM Fig2A loss of spg1 function does not affect cdc7 kinase activity ------- COMMENT: 709eb266115f50d4 7 YbvnrWrKjI53aMDHFnKMMxBNnyw Fig3A,C spg1-HA observed at SPB throughout the mitotic cell cycle ------- COMMENT: 709eb266115f50d4 11 s8zxygB4j9+cKrsniSSlYhY+9P0 Fig5A ------- COMMENT: 709eb266115f50d4 12 s8zxygB4j9+cKrsniSSlYhY+9P0 Fig5A ------- COMMENT: 709eb266115f50d4 13 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: 709eb266115f50d4 14 1J48IUFBzbYF2nj8G4pnipiFcUE Fig 6A in late anaphase cdc7 is normally localized only one SPB ------- COMMENT: 709eb266115f50d4 15 VsC/srIahxVdUF00NUsxJtpm5hA Fig 6C in late anaphase cdc7 is normally localized only one SPB ------- COMMENT: 709eb266115f50d4 19 XjaBcCAQhuYBsHt8KrYohYGQqMc cdc7 is associated with both SPBs when a short spindle is present ------- COMMENT: 709eb266115f50d4 21 F2zn5UdsqPHhbd1uzDnWj6Filoo (comment: From this paper we don't actuallyyet know that it's the new one, that comes later, but ..)cdc7 is associated with both SPBs when a short spindle is present) ------- COMMENT: 709eb266115f50d4 22 9bHK75NDpTZGjjDyF3KjjZR+KSs (comment: GTP bound) ------- COMMENT: 709eb266115f50d4 23 9bHK75NDpTZGjjDyF3KjjZR+KSs (comment: GTP bound) ------- COMMENT: 709eb266115f50d4 24 9bHK75NDpTZGjjDyF3KjjZR+KSs (comment: GTP bound) ------- COMMENT: 711d04e89064d5c4 6 sgMmi3wK+dJBlkGL3QsG5hsV9c0 Fig. 1a ------- COMMENT: 711d04e89064d5c4 7 sgMmi3wK+dJBlkGL3QsG5hsV9c0 Fig. 1a ------- COMMENT: 711d04e89064d5c4 8 gb7DgwEfPqpvSSwDFuzUnD7Guhg Fig. 1c,d ------- COMMENT: 711d04e89064d5c4 9 Axs1T6sc/VTv9NoOw/OOuxwIAh0 Fig. 2a,b and Extended Data Fig. 2a ------- COMMENT: 711d04e89064d5c4 10 Axs1T6sc/VTv9NoOw/OOuxwIAh0 Fig. 2a,b and Extended Data Fig. 2a ------- COMMENT: 711d04e89064d5c4 11 Axs1T6sc/VTv9NoOw/OOuxwIAh0 Fig. 2a,b and Extended Data Fig. 2a ------- COMMENT: 711d04e89064d5c4 12 Axs1T6sc/VTv9NoOw/OOuxwIAh0 Fig. 2a,b and Extended Data Fig. 2a ------- COMMENT: 711d04e89064d5c4 13 Axs1T6sc/VTv9NoOw/OOuxwIAh0 Fig. 2a,b and Extended Data Fig. 2a ------- COMMENT: 711d04e89064d5c4 14 Axs1T6sc/VTv9NoOw/OOuxwIAh0 Fig. 2a,b and Extended Data Fig. 2a ------- COMMENT: 711d04e89064d5c4 15 Axs1T6sc/VTv9NoOw/OOuxwIAh0 Fig. 2a,b and Extended Data Fig. 2a ------- COMMENT: 711d04e89064d5c4 16 Axs1T6sc/VTv9NoOw/OOuxwIAh0 Fig. 2a,b and Extended Data Fig. 2a ------- COMMENT: 711d04e89064d5c4 17 DW9oVq+RaaI5aBDTf1Psevgn8TY (Fig. 2e) Fig. 2f) Fig. 3a,b). ------- COMMENT: 711d04e89064d5c4 18 claZotsGb1Fk2t/vg1ldJzXIso8 Fig. 1c,d ------- COMMENT: 711d04e89064d5c4 20 eNDD+3Nl5kkwbIol7GZvm4EqtYQ (Fig. 3b), suggesting that cohesin in principle achieves topological loading onto DNA independently of a cohesin loader, albeit inefficiently. ------- COMMENT: 711d04e89064d5c4 21 nBJVCz2TYqYcRLFNA8kTQoRhtXQ (Fig. 2e) Fig. 2f) Fig. 3a,b). ------- COMMENT: 711d04e89064d5c4 22 eNDD+3Nl5kkwbIol7GZvm4EqtYQ (Fig. 3b), suggesting that cohesin in principle achieves topological loading onto DNA independently of a cohesin loader, albeit inefficiently. ------- COMMENT: 711d04e89064d5c4 23 eNDD+3Nl5kkwbIol7GZvm4EqtYQ (Fig. 3b), suggesting that cohesin in principle achieves topological loading onto DNA independently of a cohesin loader, albeit inefficiently. ------- COMMENT: 711d04e89064d5c4 24 eNDD+3Nl5kkwbIol7GZvm4EqtYQ (Fig. 3b), suggesting that cohesin in principle achieves topological loading onto DNA independently of a cohesin loader, albeit inefficiently. ------- COMMENT: 711d04e89064d5c4 27 AwmSPF/sGX31E3z0qk4h769rlKg fig 4 b ------- COMMENT: 71258840c50eb005 2 b+00gXuAJVmG6MoHxSub6s9WxVw Figure 4C ------- COMMENT: 71258840c50eb005 3 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: 71258840c50eb005 4 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 71258840c50eb005 5 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 71258840c50eb005 6 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 71258840c50eb005 7 rI8g1ktTDBN/1s3YKqCB+XXrKnw (comment: CHECK ~35% zygote of this mutant underwent equational division in meiosis I) ------- COMMENT: 71258840c50eb005 8 y7rmiLBb1JuNymnsCUDAvDwhf9o Figure 3C. For the homologs that segregated reductionally in psm3-K105R/K106R/K1013R cells, ~50% showed nondisjunction in meiosis II, consistent with random segregation. This suggests that psm3-K105R/K106R/K1013R cells are defective in pericentric cohesion (or cohesion protection) in addition to core centromeric cohesion. ------- COMMENT: 71284b5f82561435 2 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: 71284b5f82561435 3 JJ61Rr+BzZ+yYkS4ndaMB26Ouxc Fig 2B ------- COMMENT: 71284b5f82561435 4 JJ61Rr+BzZ+yYkS4ndaMB26Ouxc Fig 2B ------- COMMENT: 71284b5f82561435 5 JJ61Rr+BzZ+yYkS4ndaMB26Ouxc Fig 2B ------- COMMENT: 71284b5f82561435 6 JJ61Rr+BzZ+yYkS4ndaMB26Ouxc Fig 2B ------- COMMENT: 71284b5f82561435 7 ZVEbdgUuPB2v1iWSSxSF2AsRJEc figure 2C ------- COMMENT: 71284b5f82561435 8 ZVEbdgUuPB2v1iWSSxSF2AsRJEc figure 2C ------- COMMENT: 71284b5f82561435 9 FGLZyz0AtytP5MxZymuv5gLFmmc figure 3a (comment: CHECK cell cycle arrest in mitotic metaphase) ------- COMMENT: 71284b5f82561435 11 T72FZCApPHkrBel/SxObTqm++wU figure 3a (comment: CHECK cell cycle arrest in mitotic metaphase) ------- COMMENT: 71284b5f82561435 12 BPUcUFJ6w7ZOM75eZQQsX8FnLQs figure 3a (comment: CHECK DECREASED cell cycle arrest in mitotic anaphase) ------- COMMENT: 71284b5f82561435 14 H0MnaHI1FiMjP1CYNeqjyYIN72Q figure 5A ------- COMMENT: 7145919e461c7ff3 4 v9wxAWmxjv1538wSl7/083ytFF8 assayed with Ub-Pcn1 fusion; wild type Pcn1 absent; Pcn1-K164R present but previously shown not to be ubiquitinated at all ------- COMMENT: 7145919e461c7ff3 5 v9wxAWmxjv1538wSl7/083ytFF8 assayed with Ub-Pcn1 fusion; wild type Pcn1 absent; Pcn1-K164R present but previously shown not to be ubiquitinated at all ------- COMMENT: 7145919e461c7ff3 6 v9wxAWmxjv1538wSl7/083ytFF8 assayed with Ub-Pcn1 fusion; wild type Pcn1 absent; Pcn1-K164R present but previously shown not to be ubiquitinated at all ------- COMMENT: 7145919e461c7ff3 7 v9wxAWmxjv1538wSl7/083ytFF8 assayed with Ub-Pcn1 fusion; wild type Pcn1 absent; Pcn1-K164R present but previously shown not to be ubiquitinated at all ------- COMMENT: 7145919e461c7ff3 8 v9wxAWmxjv1538wSl7/083ytFF8 assayed with Ub-Pcn1 fusion; wild type Pcn1 absent; Pcn1-K164R present but previously shown not to be ubiquitinated at all ------- COMMENT: 7145919e461c7ff3 9 v9wxAWmxjv1538wSl7/083ytFF8 assayed with Ub-Pcn1 fusion; wild type Pcn1 absent; Pcn1-K164R present but previously shown not to be ubiquitinated at all ------- COMMENT: 7145919e461c7ff3 10 v9wxAWmxjv1538wSl7/083ytFF8 assayed with Ub-Pcn1 fusion; wild type Pcn1 absent; Pcn1-K164R present but previously shown not to be ubiquitinated at all ------- COMMENT: 7145919e461c7ff3 11 v9wxAWmxjv1538wSl7/083ytFF8 assayed with Ub-Pcn1 fusion; wild type Pcn1 absent; Pcn1-K164R present but previously shown not to be ubiquitinated at all ------- COMMENT: 7145919e461c7ff3 16 ITO695x9nnZCEXqEEz/5vguX5AQ (comment: PCNA trimerization) ------- COMMENT: 7145919e461c7ff3 17 ITO695x9nnZCEXqEEz/5vguX5AQ (comment: PCNA trimerization) ------- COMMENT: 714b38967995ac5b 1 gxgFTvLhlhrpDx43jGBeZaqNCKE Fig. 1B Fig. 1C (14.04􏰃0.25 versus 11.98􏰃0.29􏰋m, respectively) ------- COMMENT: 714b38967995ac5b 7 wj2VSE893AMBlEWR8+9Ey55om+8 Fig 2B ------- COMMENT: 714b38967995ac5b 13 PTpMYwh2+s8bXNDA7b0eFL810NQ (Fig. 1D). In addition, basal Sty1 activity was significantly higher in exponentially growing rnc1􏰂 cells ex- pressing a genomic C-terminal hemagglutinin (HA)-tagged version of the MAP kinase, compared to wild-type cells or a pmk1􏰂 mutant ------- COMMENT: 714b38967995ac5b 15 PbVAOwijuNaiG5wus0soAnKSjRU fig 3f ------- COMMENT: 714b38967995ac5b 17 yhm5gldwcfhiTVdMfXfJ7abViCA Fig2 ------- COMMENT: 714b38967995ac5b 40 I6LboU4Lw6xVx+Dw0i0iWz2pKDM Pyp2 protein levels increased 􏰁2 times in the mutant background (Fig. 5C), but they were of a lower magnitude than that in rnc1􏰂 versus wild-type cells (􏰁8 to 9 times) (Fig. 2C ------- COMMENT: 714b38967995ac5b 44 TU1kaif559//pN6KhSOWklkJ5Yc ------- COMMENT: 714b38967995ac5b 45 4LvpwOYEVIBzwElVS/YLEnyFv4w ------- COMMENT: 714b38967995ac5b 46 TU1kaif559//pN6KhSOWklkJ5Yc ------- COMMENT: 714b38967995ac5b 50 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 714b38967995ac5b 51 j2KqeifxSGw3vAyl0+dfwkIKUAY fig2C ------- COMMENT: 714b38967995ac5b 52 S0timjkd1LJkK4GhURIMYwvBG7I (comment:12.13 + 0.1) ------- COMMENT: 714b38967995ac5b 56 3oQVe2HxNI+v5oTDmvRuA3rr2ls fig 4h ------- COMMENT: 714b38967995ac5b 63 MRTl98rKQY4u69JsQq5X5gUaN4M fig 5C ------- COMMENT: 714b38967995ac5b 68 pZSJQXvoT1cUExD0UaNqQ5922mo fig 5e ------- COMMENT: 714b38967995ac5b 69 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 714b38967995ac5b 85 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 714b38967995ac5b 91 lwvtzHZDwUJr4l2PQNJlnYkvnjo Fig. 1B cell length at division either of pmk1􏰂 cells or in a mutant strain lacking the dual-specificity phos- phatase Pmp1 that dephosphorylates and inactivates Pmk1 in vivo (14), ... was similar to that of wild-type cells (Fig. 1B) ------- COMMENT: 714b38967995ac5b 92 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 714b38967995ac5b 93 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 714b38967995ac5b 94 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 714b38967995ac5b 96 fxMvAbPD4bqeysUNHyiZEgnYVQI These results suggest that while T50 is a main phosphorylation site for Sty1 within Rnc1, other phosphosites are likely targeted by this kinase in vivo. ------- COMMENT: 714b38967995ac5b 99 TU1kaif559//pN6KhSOWklkJ5Yc ------- COMMENT: 714b38967995ac5b 100 dPuDqQWZsmgZDRnfopuEJjTaGYU A nonphosphorylatable GST-Rnc1 fusion [GST-Rnc1(S/T6A)] expressed in fission yeast was several times less effective than the wild type (GST-Rnc1) in binding wak1􏰀, wis1􏰀 , atf1􏰀 , pyp1􏰀 , and pyp2􏰀 mRNAs in vitro (Fig. 5A) ------- COMMENT: 714b38967995ac5b 101 dPuDqQWZsmgZDRnfopuEJjTaGYU A nonphosphorylatable GST-Rnc1 fusion [GST-Rnc1(S/T6A)] expressed in fission yeast was several times less effective than the wild type (GST-Rnc1) in binding wak1􏰀, wis1􏰀 , atf1􏰀 , pyp1􏰀 , and pyp2􏰀 mRNAs in vitro (Fig. 5A) ------- COMMENT: 714b38967995ac5b 102 dPuDqQWZsmgZDRnfopuEJjTaGYU A nonphosphorylatable GST-Rnc1 fusion [GST-Rnc1(S/T6A)] expressed in fission yeast was several times less effective than the wild type (GST-Rnc1) in binding wak1􏰀, wis1􏰀 , atf1􏰀 , pyp1􏰀 , and pyp2􏰀 mRNAs in vitro (Fig. 5A) ------- COMMENT: 714b38967995ac5b 103 dPuDqQWZsmgZDRnfopuEJjTaGYU A nonphosphorylatable GST-Rnc1 fusion [GST-Rnc1(S/T6A)] expressed in fission yeast was several times less effective than the wild type (GST-Rnc1) in binding wak1􏰀, wis1􏰀 , atf1􏰀 , pyp1􏰀 , and pyp2􏰀 mRNAs in vitro (Fig. 5A) ------- COMMENT: 714b38967995ac5b 104 dPuDqQWZsmgZDRnfopuEJjTaGYU A nonphosphorylatable GST-Rnc1 fusion [GST-Rnc1(S/T6A)] expressed in fission yeast was several times less effective than the wild type (GST-Rnc1) in binding wak1􏰀, wis1􏰀 , atf1􏰀 , pyp1􏰀 , and pyp2􏰀 mRNAs in vitro (Fig. 5A) ------- COMMENT: 714b38967995ac5b 105 a4WqZY/2ExzPbpdC59NSqBXJaT4 and enhanced expression of Wak1, Wis1, and Pyp1 proteins during unperturbed growth (Fig. 5C) ------- COMMENT: 7169c40527631f2c 18 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment:same as either single mutant) ------- COMMENT: 7169c40527631f2c 19 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment:same as either single mutant) ------- COMMENT: 7169c40527631f2c 20 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment:same as either single mutant) ------- COMMENT: 7169c40527631f2c 23 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment:same as either single mutant) ------- COMMENT: 7169c40527631f2c 24 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment:same as either single mutant) ------- COMMENT: 7186b912b1fbca8b 2 kGeOzbFcTUaiwvvY1pzlEJ5VhL8 (comment: CHECK **SYNTHETIC LETHAL) ------- COMMENT: 7186b912b1fbca8b 8 ZWwvOAJhjQ0/kr9r3bedR2r+1jM (comment: CONDITION toxic aa-analog) ------- COMMENT: 719521c232a83155 1 rzyqYgfia7yqjHV/K4iXeE2zfLM (comment: CONDITION at 37C) ------- COMMENT: 719521c232a83155 2 rzyqYgfia7yqjHV/K4iXeE2zfLM (comment: CONDITION at 37C) ------- COMMENT: 719521c232a83155 3 rzyqYgfia7yqjHV/K4iXeE2zfLM (comment: CONDITION at 37C) ------- COMMENT: 719521c232a83155 4 rzyqYgfia7yqjHV/K4iXeE2zfLM (comment: CONDITION at 37C) ------- COMMENT: 719521c232a83155 16 Rb1A3GakLTHKlUodgyjzvoiNMPI (comment: CHECK TBZ) ------- COMMENT: 719521c232a83155 17 c3H/3XXIg9zyBkaTCGSZXF3ruSU silencing defect at the outher centromeric region ------- COMMENT: 719521c232a83155 18 Rb1A3GakLTHKlUodgyjzvoiNMPI (comment: CHECK TBZ) ------- COMMENT: 719521c232a83155 19 c3H/3XXIg9zyBkaTCGSZXF3ruSU silencing defect at the outher centromeric region ------- COMMENT: 719521c232a83155 20 Rb1A3GakLTHKlUodgyjzvoiNMPI (comment: CHECK TBZ) ------- COMMENT: 719521c232a83155 23 fIgB0qdf14lJfp+f7Ri+siFnTbE (comment: genome-wide average) ------- COMMENT: 719521c232a83155 24 YWwKDZ1P7M9x2Qrm3vMDYZa0HAg (comment: genome-wide average; slightly increased amplitudes of the -2, -1, +1 nucleosome peaks (relative to NDR) ------- COMMENT: 719521c232a83155 61 rzyqYgfia7yqjHV/K4iXeE2zfLM (comment: CONDITION at 37C) ------- COMMENT: 719521c232a83155 62 KV7UDDXMkPXK9TlBRr+q2DKA+yY ------- COMMENT: 71e50efc69bcd7d5 1 iJjQZcnth1RBxGcibySwvOIKSPY (comment: Observed with probe for active Cdc42 (CRIB)) ------- COMMENT: 71e50efc69bcd7d5 6 iJjQZcnth1RBxGcibySwvOIKSPY (comment: Observed with probe for active Cdc42 (CRIB)) ------- COMMENT: 71e50efc69bcd7d5 14 T8bErFw9QYSAss1FgqueDAIaE2I (comment: inferred from localization plus GTPase activity) ------- COMMENT: 71e68c1b5b6dc976 12 yJmZF51XSxaGoRHYRlLokx0IbZE (comment: same as swi7-H4 alone, i.e. it's dominant) ------- COMMENT: 720a7afb1d87000c 44 f/OSDtZd0yBmD83jZzZr/y+/Jho Consistently, the phenotype of cut9-T98 was indistinguishable from that of cut9-665 ------- COMMENT: 720a7afb1d87000c 45 f/OSDtZd0yBmD83jZzZr/y+/Jho Consistently, the phenotype of cut9-T98 was indistinguishable from that of cut9-665 ------- COMMENT: 720a7afb1d87000c 46 f/OSDtZd0yBmD83jZzZr/y+/Jho Consistently, the phenotype of cut9-T98 was indistinguishable from that of cut9-665 ------- COMMENT: 721bf4355105bfb4 8 71yEvK2qeXXzCSbc9WulMnBYSaA localizes to division site before Scd1, and before contractile ring constriction begins ------- COMMENT: 721bf4355105bfb4 9 71yEvK2qeXXzCSbc9WulMnBYSaA localizes to division site before Scd1, and before contractile ring constriction begins ------- COMMENT: 721bf4355105bfb4 10 9zFFwwamd2KLH2hq67g07jXcKpA localizes to division site after Gef1 and Scd2, but before contractile ring constriction begins ------- COMMENT: 721bf4355105bfb4 30 LzDRNL7k4MwbZtNs3p+zxhjyIbE cdc42-G12V suppresses localization of scd1 phenotype of gef1delta ------- COMMENT: 722def8c5161e3f1 151 GyA7QzXVWNjR4Yo/S077fGQy4z4 detectable in mutants that increase bound GTP:GDP ratio, implying that protein-protein interaction is GTP-dependent ------- COMMENT: 72405b0ba0db4d73 2 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: 72405b0ba0db4d73 3 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: 72405b0ba0db4d73 4 9961hfGTWu8r0QbEJ9OhS3HVSI0 highly bent or branched morphology (Figure 2a) (penetrance from Figure 6B) ------- COMMENT: 72405b0ba0db4d73 5 9961hfGTWu8r0QbEJ9OhS3HVSI0 highly bent or branched morphology (Figure 2a) (penetrance from Figure 6B) ------- COMMENT: 72405b0ba0db4d73 6 M9rbOuESD3nRZhmfvaozfJPQeGE On the other hand, oxidative stress by hydrogen peroxide, which also induces Spc1 activation [34], did not significantly affect Δwsh3 cells (data not shown). ------- COMMENT: 72405b0ba0db4d73 8 hVBF0JlxBq71UOcxW8PFL8HBUQ0 (Figure 2B) ------- COMMENT: 72405b0ba0db4d73 9 /P9aN1/TFWM96w5dljecO/1+Cmw 2c Δwsh3 cells were found to grow exclusively in a monopolar fashion. ------- COMMENT: 72405b0ba0db4d73 10 cMrpFwowUDz9gVqF9O0fiWNthNU Actin patches, which are localized to the growing tips of fission yeast cells [38], were detected mostly in one tip of the Δwsh3 cell (Figure 2C). ------- COMMENT: 72405b0ba0db4d73 13 66TzU6Ot3OaEj4iovMuVCq+C5jk Wsh3-GFP was abrogated by a mutation in β- tubulin, nda3-KM311 [39] even at its permissive temperature, 30oC (Figure 3B, left). ------- COMMENT: 72405b0ba0db4d73 14 OuO9hSAyTlOBNX7DFXVrLlNUZPg The specific localization of Wsh3-GFP was lost in the Δtea1 mutant and the Wsh3-GFP signal was diffused throughout the cytoplasm (Figure 4A) ------- COMMENT: 72405b0ba0db4d73 16 UUAc6gZtemlvLasUw4lNM3RZh7M In contrast, most of Δwsh3 cells showed highly concentrated Tea1-GFP signals at one end, while the other end contained significantly less Tea1-GFP dots (Figure 5A, right panel) ------- COMMENT: 72405b0ba0db4d73 17 Ya5TE8ChhE+4ljVqp3MT2JNdaeA Δwsh3 cells, the cell-end localization of Pom1 was lost and Pom1-GFP often accumulated in vesicle-like structures in the cytoplasm (Figure 5D) ------- COMMENT: 72405b0ba0db4d73 19 y1I5hXVUkl3xGH3gC4oWgWLlFx0 (penetrance from 6B) nWe found that high osmolarity stress by 0.6 M KCl also promotes appearance of T-shaped cells in the Δtea1 strain, to the levels comparable to the Δwsh3 mutant (Figure 6B). ------- COMMENT: 72405b0ba0db4d73 20 5+6SMYf9hpTTARxCv95yFzqBeKE Cell polarity defects with bent and branched morphology were observed after shifting the Δspc1 mutant from 25oC to 36oC (Figure 6C). ------- COMMENT: 72405b0ba0db4d73 21 +vFTer9/g5Gt8sBvUU21QBsqzjU We also found that, even under the optimal growth condition, the Δspc1 mutation exacerbates the morphology defects of the Δtea1 mutant; the Δtea1 Δspc1 double mutant grown at 30oC in rich YES medium contain large fractions of significantly bent and branched cells (Figure 6D). ------- COMMENT: 72405b0ba0db4d73 22 +vFTer9/g5Gt8sBvUU21QBsqzjU We also found that, even under the optimal growth condition, the Δspc1 mutation exacerbates the morphology defects of the Δtea1 mutant; the Δtea1 Δspc1 double mutant grown at 30oC in rich YES medium contain large fractions of significantly bent and branched cells (Figure 6D). ------- COMMENT: 72405b0ba0db4d73 23 dpxB8dMFi8LfDdzr8bNliIfCKEA (penetrance from 6B) highly bent or branched morphology (Figure 2a) ------- COMMENT: 72862a5b1395f450 3 hARLHXsSx2nKoEeXX1uJPlMD/HU three-hybrid assay involving Uaf2, Prp2, and an RNA fragment containing the heterologous beta-globin 3′ splice site ------- COMMENT: 72862a5b1395f450 4 hARLHXsSx2nKoEeXX1uJPlMD/HU three-hybrid assay involving Uaf2, Prp2, and an RNA fragment containing the heterologous beta-globin 3′ splice site ------- COMMENT: 72862a5b1395f450 5 hARLHXsSx2nKoEeXX1uJPlMD/HU three-hybrid assay involving Uaf2, Prp2, and an RNA fragment containing the heterologous beta-globin 3′ splice site ------- COMMENT: 72862a5b1395f450 6 hARLHXsSx2nKoEeXX1uJPlMD/HU three-hybrid assay involving Uaf2, Prp2, and an RNA fragment containing the heterologous beta-globin 3′ splice site ------- COMMENT: 72862a5b1395f450 8 hARLHXsSx2nKoEeXX1uJPlMD/HU three-hybrid assay involving Uaf2, Prp2, and an RNA fragment containing the heterologous beta-globin 3′ splice site ------- COMMENT: 729f627c0beaa1b2 2 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: 729f627c0beaa1b2 4 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: 729f627c0beaa1b2 5 fYqQOpzikopc+3AWAEPEUkYII7w figure 4 ------- COMMENT: 729f627c0beaa1b2 6 rcyfyqDl+Tvg7TF6X/T5T2esPDE figure 4 (comment: spindle is still present, normally disassembled by cytokinesis) ------- COMMENT: 729f627c0beaa1b2 13 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: 729f627c0beaa1b2 14 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: 729fd40714dad360 3 h1ytoEgBucGWJqqzvY2G0SmEcGc ------- COMMENT: 729fd40714dad360 9 bkKuDMQGTJ69i8Wtw/JU4HGR8hQ ------- COMMENT: 72c1277f6497035f 2 QGsIrdKYh0PwBVE+nvrH2Enokkg figure 1a ------- COMMENT: 72c1277f6497035f 3 kB+EBPu80XwQ2a0IFAU7bZsNTqA fig1 Lose telomere signal, much like trt1∆ cells. ------- COMMENT: 72c1277f6497035f 4 qoNnpot/3HHhBCcmWeAfIIwiXsw figure 1a Telomere shortening is similar to pof8∆ cells. ------- COMMENT: 72c1277f6497035f 7 jgrcDrNDw0ORn/nOZZIpcAZarLg pof8∆ taz1∆ showed much shorter telomere length (almost like wild-type cells) than taz1∆ cells, but showed some rearrangement in sub-telomeres. ------- COMMENT: 72c1277f6497035f 9 3ff828d35apFTqX+iG6wubOOce8 pof8∆ rap1∆ cells showed shortened telomeres, more similar to pof8∆, rather than highly elongated telomeres in rap1∆ cells. ------- COMMENT: 72c1277f6497035f 11 yLG6SoGiT34eoNgqj+Id5SVaExo pof8∆ poz1∆ cells showed very slightly shortend telomeres, rather than highly elongated telomeres in poz1∆ cells. (This strain showed longer telomere than pof8∆ cells.) ------- COMMENT: 72c1277f6497035f 13 IvtCAzFKGPm7Q51H3gNiuMB4wpg pof8∆ rif1∆ cells showed short telomeres, very similar to pof8∆ cells. ------- COMMENT: 72c1277f6497035f 16 S31FoOvBS1BPDxPQtj2ySr4kY/s fig 1CD Trt1-myc ChIP ------- COMMENT: 72c1277f6497035f 18 lDexoz/+wOx3jZZc3CpDqAb3dKY Based on ChIP, ter1∆ cause loss of telomerase (Trt1) localization at telomeres. ------- COMMENT: 72c1277f6497035f 19 HGDAmoylxEk58BMAVQO2PA2uBvw Trt1 expression level detected by western blot is reduced in ter1∆ cells. ------- COMMENT: 72c1277f6497035f 20 OoYzUcS0zu6ueYu1hF1wR4H3ac0 moderately reduced less severe thanin ter1∆ cells. ------- COMMENT: 72c1277f6497035f 23 QSgoSd9AbBioIwojkRO0hEvXmEM Telomere binding of Est1 is reduced in pof8∆ cells. ------- COMMENT: 72c1277f6497035f 24 XCXb2K1g7h1t93P95F21N+cCGbg Est1 showed similar expression level in pof8∆ cells as wild-type cells. ------- COMMENT: 72c1277f6497035f 25 yWdL52jCFcZ1/qjddM2M2VMKUeQ Fig. 3c Interaction between Est1 and TER1 was not affected by pof8∆. ------- COMMENT: 72c1277f6497035f 26 YBFBut5qi9nGLEjnRFEx94qv4ks fig 3b ------- COMMENT: 72c1277f6497035f 27 C11lsCZBNF049DTZjP8wFcfQJSo Est1 binding to telomeres is reduced to near no binding in ter1∆, based on ChIP assay. ------- COMMENT: 72c1277f6497035f 28 vcECO4B5NzvEzzWPrFKKeC6V/Oo Est1 expression level detected by western in ter1∆ cells was similar to Est1 level in ter1+ (wild-type) cells. ------- COMMENT: 72c1277f6497035f 34 FjBoFdHRmaUmQ+9SFHNcdYZgM+A fig 1e Localization of Pof8 at telomeres is reduced but not eliminated in ter1∆. ------- COMMENT: 72c1277f6497035f 35 cIsqRQa/f9Ys6gH7xtZE0oLR2Mc Pof8 expression level was not altered in ter1∆ cells. ------- COMMENT: 72c1277f6497035f 37 H4ol9GvV+ebp8f5KugxPrK2LSNA fig 1e Localization of Pof8 at telomeres is reduced but not eliminated in ccq1∆. ------- COMMENT: 72c1277f6497035f 38 ttQgkjPtL+97xrL09pCGNbDmFQ4 Pof8 expression level is not affected in ccq1∆. ------- COMMENT: 72c1277f6497035f 39 aLMQgzPC3MazGdap1e9rB9W1hMA fig 1e Localization of Pof8 at telomeres is reduced but not eliminated in trt1∆. ------- COMMENT: 72c1277f6497035f 40 xMTIGoFq2qlnKPFKxNbpol54PFo Pof8 expression level is not affected by trt1∆. ------- COMMENT: 72c1277f6497035f 41 odrMSmWu4wjVs0IStH9iuP4I4R8 fig 1e Localization of Pof8 at telomeres is reduced but not eliminated in est1∆. ------- COMMENT: 72c1277f6497035f 42 eUd17/P9O6TDg9ciNDLhfYKLCWA Pof8 expression was not affected by est1∆. ------- COMMENT: 72c1277f6497035f 43 v0aGZCUwu7SZz9Oe8n5Ryf80BtA Fig. 3a Pof8-TER1 interaction is reduced but not eliminated in ccq1∆. ------- COMMENT: 72c1277f6497035f 44 eAh+j+rnAxw0GXwVac29Rj2jYd4 Fig. 3a Pof8-TER1 interaction is reduced but not eliminated in trt1∆ cells. ------- COMMENT: 72c1277f6497035f 45 Fin1SEB3MsbrwD1YWHIsqnf/mgs Fig. 3a Pof8-TER1 interaction is not affected by est1∆. ------- COMMENT: 72c1277f6497035f 46 acZkuHb+JmBNR6ducM2Ckw7wqCk Trt1-TER1 interaction is reduced but not eliminated in pof8-∆[289-4020]. Extent of reduction in Trt1-TER1 is similar to pof8∆ cells. ------- COMMENT: 72c1277f6497035f 48 QkPrXh7rwVVur9qw1/hs0CdDJvo Fig 4a Expression level of telomerase RNA TER1 is reduced but not eliminated in pof8∆ cells. Expression level for telomerase RNA pre-cursor was not affected by pof8∆. ------- COMMENT: 72c1277f6497035f 50 4iBHvlHNb6gMFXuTKD1ThBNPecY Fig. 4c Lsm3-TER1 interaction is abolished in pof8∆ cells. ------- COMMENT: 72c1277f6497035f 53 zYNlNdmm9eCYhXO5kdpbQyDzr4k Fig. 4f ------- COMMENT: 72c1277f6497035f 56 zdDLyq8xmDcfqg3xuocPheON4oo Lsm3 protein level was not affected by pof8∆. ------- COMMENT: 72c1277f6497035f 57 UdF2LfKWSU3QXEwO3iEdKjUSViQ Fig. 4f Lsm3 binding at telomeres is increased by ter1∆. ------- COMMENT: 72c1277f6497035f 58 IAiqEIVSLgITG+5/FwmELpYUVIs Lsm3 binding was detected at ars2004, non-ARS, ade6+ and his1+ loci. In ter1∆ cells, Lsm3 binding to those non-telmeric sites were increased. ------- COMMENT: 72c1277f6497035f 59 2+jvSNKikfvlBxTwHMQrWxF7++Y Lsm3 protein level was not affected by ter1∆. ------- COMMENT: 72c1277f6497035f 60 CUQx2jt4YUBKBb6xrdf+B4fUEXI Fig. 4f Lsm3 binding to telomeres was not affected by trt1∆. ------- COMMENT: 72c1277f6497035f 61 cLGtZJGDhhdfiPAVfubGpwXl2HI Lsm3 protein level was not affected by trt1∆. ------- COMMENT: 72c1277f6497035f 62 3nMRrQ4p04RUPKs9nyKAUR7sE9g Fig. 4f Lsm3 binding to telomeres was not affected by est1∆. ------- COMMENT: 72c1277f6497035f 63 8QZtBP/njvFcKNHxJwDT1g2WVlU Lsm3 protein level was not affected by est1∆. ------- COMMENT: 72c1277f6497035f 66 NOecXQmUdKw3ue9XajAeXxyFvEA Fig. 4d ------- COMMENT: 72c1277f6497035f 68 kYrV4rGR3n5Jqn79Ftv3+1XhUAU Fig. 4e ------- COMMENT: 72c1277f6497035f 69 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 72c1277f6497035f 70 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 72c1277f6497035f 71 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 72c1277f6497035f 72 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 72c1277f6497035f 75 gXscRF6aLlh4cKi08jcFfkjzxbQ Trt1 binding is reduced to ~69% of pof8+ cells, but not as severely reduced as pof8∆ cells (~35%). ------- COMMENT: 72c1277f6497035f 76 hEpdQSqo7JSDAqNrz8e0hQA0Sks fig 6 pof8-Y330A cells show as short telomere as pof8∆ cells. ------- COMMENT: 72c1277f6497035f 77 SRaXICiIPe6eI4OO7v9oR6e+H4Q fig 6 pof8-R343A cells show as short telomere as pof8∆ cells. ------- COMMENT: 72c1277f6497035f 78 za40yFVtqM7KqzV2l9Xn20Cnv84 fig 6 pof8-∆[390-402] cells show as short telomere as pof8∆ cells. ------- COMMENT: 72c1277f6497035f 79 xsEGmJX2aD0VwGa+rtVDQar+NcM fig 6 pof8-∆[289-402] cells show as short telomere as pof8∆ cells. ------- COMMENT: 72c1277f6497035f 81 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: 72c1277f6497035f 82 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: 72c1277f6497035f 83 VMESfstBuFagFlbRIQT7UqQGkSI fig 6b ------- COMMENT: 72c1277f6497035f 97 /LLrHY0GyPx9UfvJ3RIA3bGCJk4 Trt1 binding is reduced to ~58% of pof8+ cells, but not as severely reduced as pof8∆ cells (~35%). ------- COMMENT: 72c1277f6497035f 99 W4dYkAmC5EbC5GEuoRBe3716Z4c Trt1 binding is reduced to ~70% of pof8+ cells, but not as severely reduced as pof8∆ cells (~35%). ------- COMMENT: 72c1277f6497035f 101 0H9Hz41jwUbACJpaHn3BlZADknE Trt1 binding is reduced to ~80% of pof8+ cells, but not as severely reduced as pof8∆ cells (~35%). ------- COMMENT: 72c1277f6497035f 126 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 72c1277f6497035f 127 iF1VrVXm5+4hsUg8PuwWmtVR29s Fig. 1e ------- COMMENT: 72c1277f6497035f 129 ygva1KLVMAo2mNSESCgCd04BlAE fig 1C,D ------- COMMENT: 72c1277f6497035f 130 ygva1KLVMAo2mNSESCgCd04BlAE fig 1C,D ------- COMMENT: 72c1277f6497035f 131 ygva1KLVMAo2mNSESCgCd04BlAE fig 1C,D ------- COMMENT: 72c1277f6497035f 132 jm8mYL7A83JV0f+8wo95EwZUvb8 fig 1C,D ------- COMMENT: 72c1277f6497035f 133 jm8mYL7A83JV0f+8wo95EwZUvb8 fig 1E ------- COMMENT: 72c1277f6497035f 134 jm8mYL7A83JV0f+8wo95EwZUvb8 fig 1E ------- COMMENT: 72c1277f6497035f 136 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: 72c1277f6497035f 137 J6/0wvE2yMKyXpYLPy//94yUWTg fig 3a ------- COMMENT: 72c1277f6497035f 138 x6rMWQ6kb46Nq/Zeft4z2cRsgf4 ------- COMMENT: 72c1277f6497035f 139 5Pzn4TrbQxIKWB3nJQzOY7/e9yE ------- COMMENT: 72c1277f6497035f 140 0P6PNV61Mi7VBqPeKRvc2YdCLU8 fig 3 b ------- COMMENT: 72c1277f6497035f 141 igy+QMUpfYzBZE0UDw3dlZBp21s ------- COMMENT: 72c1277f6497035f 142 Yx3h6BWV6gnCiod15Lr4eLsnbEw ------- COMMENT: 72c1277f6497035f 143 hB/i1rZRVyJ1Xf7vMyfjwGsdNao ------- COMMENT: 72c1277f6497035f 144 VOZDinX9sTn7uddb3A1hFfKx49c ------- COMMENT: 72c1277f6497035f 145 VOZDinX9sTn7uddb3A1hFfKx49c ------- COMMENT: 72c1277f6497035f 146 igy+QMUpfYzBZE0UDw3dlZBp21s ------- COMMENT: 72c1277f6497035f 148 S6Ei6UT/oKacex0CmLHcP3vmM0Y figs 1-3 ------- COMMENT: 72c1277f6497035f 149 4Zqzab3UzX8IHcxf3wSa0sXT23U Supplementary Fig. 4 ------- COMMENT: 72c1277f6497035f 150 4Zqzab3UzX8IHcxf3wSa0sXT23U Supplementary Fig. 4 ------- COMMENT: 72c1277f6497035f 151 4Zqzab3UzX8IHcxf3wSa0sXT23U Supplementary Fig. 4 ------- COMMENT: 72c1277f6497035f 152 hB/i1rZRVyJ1Xf7vMyfjwGsdNao ------- COMMENT: 72c1277f6497035f 153 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: 72c1277f6497035f 157 S6Ei6UT/oKacex0CmLHcP3vmM0Y figs 1-3 ------- COMMENT: 72c1277f6497035f 160 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: 72c1277f6497035f 161 4Zqzab3UzX8IHcxf3wSa0sXT23U Supplementary Fig. 4 ------- COMMENT: 72f780a0a113152a 94 qbuz245yY2dHUiJ8DO/v+2JGATE isp7+ overexpression decreases Gad8's kinase activity towards substrate Fkh2 ------- COMMENT: 72f780a0a113152a 123 6X7Rj88mo+hooJsZQIIOjh/j1T0 same as isp7+ overexpression alone ------- COMMENT: 72f780a0a113152a 124 6X7Rj88mo+hooJsZQIIOjh/j1T0 same as isp7+ overexpression alone ------- COMMENT: 72fbfb14c00e0ddd 13 uKCmshVDBskTEAmkf7qZrHuK5IQ (comment: CHECK also present in early anaphase; disappears by late anaphase) ------- COMMENT: 72fbfb14c00e0ddd 15 uKCmshVDBskTEAmkf7qZrHuK5IQ (comment: CHECK also present in early anaphase; disappears by late anaphase) ------- COMMENT: 733ed151713320db 28 u4IHZzq9aaQbMqvsp3KrNZTNHqk ------- COMMENT: 733ed151713320db 29 u4IHZzq9aaQbMqvsp3KrNZTNHqk ------- COMMENT: 733ed151713320db 30 u4IHZzq9aaQbMqvsp3KrNZTNHqk ------- COMMENT: 733ed151713320db 31 u4IHZzq9aaQbMqvsp3KrNZTNHqk ------- COMMENT: 733ed151713320db 32 u4IHZzq9aaQbMqvsp3KrNZTNHqk ------- COMMENT: 7341b39d1e6a56ef 1 gR3uDCAKlnncwTn32hpOM/vFFBs Fig. 4A and S7 ------- COMMENT: 7341b39d1e6a56ef 2 rkTnCwVdwrjwSUoFQUTUY/3rKfA Fig. 4A and S8 ------- COMMENT: 7341b39d1e6a56ef 3 rkTnCwVdwrjwSUoFQUTUY/3rKfA Fig. 4A and S8 ------- COMMENT: 7341b39d1e6a56ef 4 xEXA2rF3YIbR0ZM/fIIhl0F9cLk abolished both kinetochore localization and SAC signaling (Fig. 1C,D), suggesting that kinetochore localization is crucial for SAC activity. ------- COMMENT: 7341b39d1e6a56ef 5 yeemavsrj1BvGnyydiXb6AjoVwQ comment: CHECK ditto ------- COMMENT: 7341b39d1e6a56ef 6 V9+ByOoFt1dlxI49Pk8R8C6l7dw The shorter truncation (Mph1-D1-150) maintained kinetochore localization and SAC signaling, ------- COMMENT: 7341b39d1e6a56ef 7 V9+ByOoFt1dlxI49Pk8R8C6l7dw The shorter truncation (Mph1-D1-150) maintained kinetochore localization and SAC signaling, ------- COMMENT: 7341b39d1e6a56ef 8 4LLB5rhBibJYT56EY9izlxSb2W8 (Fig. 4C). ------- COMMENT: 7341b39d1e6a56ef 9 gR3uDCAKlnncwTn32hpOM/vFFBs Fig. 4A and S7 ------- COMMENT: 7341b39d1e6a56ef 10 v9pgPRaGi3vKrB4GKjVT2ujySkI Mph1 localizes to unattached kinetochores in bub3D cells (Fig. 2A). ------- COMMENT: 7341b39d1e6a56ef 11 lK27pwNWYLOIRwJ9sZy8DUKTA2I Fig. 4B Fig. S5 ------- COMMENT: 7341b39d1e6a56ef 12 bFfwUNY+gDbRZtLe4Kd5GumGlyw Fig. 4A and S1 ------- COMMENT: 7341b39d1e6a56ef 13 Zb68HTxB1rftRUFbTrfNrCY0m7M Fig. 4A and S9 ------- COMMENT: 7341b39d1e6a56ef 14 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 7341b39d1e6a56ef 15 9lXNgrffSLDAj+sDNKnAX8zcoRc Fig. S6 Indeed, Ark1 and Mph1 are fully or partially required for the kinetochore enrichment of all other SAC proteins (Fig. 4A; supplementary material Figs S5–S10). ------- COMMENT: 7341b39d1e6a56ef 16 82rmuE2qaElnGkj7kcgJKzGNVCs Fig. S7 ------- COMMENT: 7341b39d1e6a56ef 17 7fbuic9J4nxECAcRt8dTP1kf/5Q Fig. S8 ------- COMMENT: 7341b39d1e6a56ef 18 jsC8DvDp1FiE9EG3/mNb2dkRIpQ (Fig. 4B) ------- COMMENT: 7341b39d1e6a56ef 19 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 7341b39d1e6a56ef 20 K1q+PpDtg+Om3QXtpxFSYyHAtxA Indeed, Ark1 and Mph1 are fully or partially required for the kinetochore enrichment of all other SAC proteins (Fig. 4A; supplementary material Figs S5–S10). ------- COMMENT: 7341b39d1e6a56ef 21 Eom/AREuey77KEIwv3rXioz/+bk Fig.3A. When proper chromosome attachment was prevented by a conditional mutation in kinesin-5 (cut7-446), Mph1 localized to kinetochores, but the enrichment was abrogated by chemical genetic inhibition of Ark1 with the small molecule 1NM-PP1 (Fig. 3A). ------- COMMENT: 7341b39d1e6a56ef 22 VHLrcArSW8pH1cn4W6HyCHBmwr8 Figure S8E and F. Indeed, Ark1 and Mph1 are fully or partially required for the kinetochore enrichment of all other SAC proteins (Fig. 4A; supplementary material Figs S5–S10). ------- COMMENT: 7341b39d1e6a56ef 23 WFSdeyToXFtF28ZLmFgH5UtSYOQ Figure S4B and C ------- COMMENT: 7341b39d1e6a56ef 24 6bVMxxkDlPeCmSmzNl3sE+DBW4Y (comment: CHECK 60% cells) Forced recruitment of wild-type Mph1 to kinetochores lead to apronounced delay in mitosis and a growth defect (Fig. 1E,F), ------- COMMENT: 7341b39d1e6a56ef 25 /3ubQIwIBtrJTl/qQyKVohPnxWM rescued by deletion of mad2, which (forced recruitment of Mph1 artificially promoted SAC signaling andthat the fusion to Mis12 did not impair kinetochore function. ------- COMMENT: 7341b39d1e6a56ef 26 npxMfhSJxhr8Lkz0yfTC02fGpqs (comment: CHECK 35% cells) (Fig. 1E,F). ------- COMMENT: 7341b39d1e6a56ef 28 UeAdYpAbq0GQCB1F+1DkltMftlw and the fraction of cells, in which a signal could be detected (supplementary material Fig. S1), were similar between bub3+ and bub3D cells ------- COMMENT: 7341b39d1e6a56ef 29 UeAdYpAbq0GQCB1F+1DkltMftlw and the fraction of cells, in which a signal could be detected (supplementary material Fig. S1), were similar between bub3+ and bub3D cells ------- COMMENT: 7341b39d1e6a56ef 30 1JnV4Zjkl0UpJnL356Z/u46h+rw In the presence of Mph1-D1-150, the SAC was still functional in bub3D cells, although the mitotic delay was shorter than in mph1-D1-150 or bub3D cells (Fig. 2C). ------- COMMENT: 7341b39d1e6a56ef 31 KVssmgiuupOn0GvKrbsfrci++ng In the presence of Mph1-D1-302, the SAC response in bub3D cells was abrogated (Fig. 2C), demonstrating that recruitment of Mph1 to kinetochores is necessary for SAC function in bub3D cells. ------- COMMENT: 7341b39d1e6a56ef 33 0hij56pqk1zu343Yd2m+n8fRBgA Together this suggests that Ark1 is directly and continuously required to maintain Mph1 localization to kinetochores. ------- COMMENT: 7341b39d1e6a56ef 40 X36cLSPaf2ikf46TcPrzQFiuycE Fig. 3B. Indeed, Ark1 and Mph1 are fully or partially required for the kinetochore enrichment of all other SAC proteins (Fig. 4A; supplementary material Figs S5–S10). ------- COMMENT: 7341b39d1e6a56ef 41 5wq82zwN0jmqXgmAqyRJUb+zZwQ (Fig. S9F) ------- COMMENT: 7341b39d1e6a56ef 42 gRGB3LomdyAooOAvikkhq6Ef0io (Fig. 3C) (inhibiting Ark1 does not rescue the Mph1-kinetochore targeting, arguing that Ark1 is upstream) ------- COMMENT: 7341b39d1e6a56ef 43 PLPUcLlz36h3ZgdfY9QUtZf+tVc (Fig. 3C)increased mitotic index (Fig. S4A) ------- COMMENT: 734a10ae784c8c6b 5 s3unKLcc+VOcN7wdKHKCzqhVXJI (comment: CHECK M-Pol I complex) ------- COMMENT: 734a10ae784c8c6b 6 s3unKLcc+VOcN7wdKHKCzqhVXJI comment: CHECK M-Pol I complex) ------- COMMENT: 734a10ae784c8c6b 14 Jpl2vaXMJYnwohm38hFbx8PalEg comment: CHECK swolle) ------- COMMENT: 734a10ae784c8c6b 21 VhR9XiY8KLNdxrG/hliXpXhBBdA figures 1,2,4. These results suggested that the elongation of mannan takes place sequentially by the actions of the a-mannosyltransferases in the order of SpOch1p, SpMnn9p and SpAnp1p. ------- COMMENT: 734a10ae784c8c6b 22 jVcNeSsr92NrI0Hlz76mFcET67M figures 1,2,4 These results suggested that the elongation of mannan takes place sequentially by the actions of the a-mannosyltransferases in the order of SpOch1p, SpMnn9p and SpAnp1p. ------- COMMENT: 734a10ae784c8c6b 27 vWGPGoMJXnBRJsH8vvujoFnBoPg figure 4. These results suggested that the elongation of mannan takes place sequentially by the actions of the a-mannosyltransferases in the order of SpOch1p, SpMnn9p and SpAnp1p. ------- COMMENT: 734a10ae784c8c6b 28 R8amQ7x3XQVMVz21dViLJ6sPsw8 (comment: split YFP and affinity capture) ------- COMMENT: 7363f18fe5e26614 24 HZQzCeympVt/Hc42NAs05y3bb7U (comment: provides the sulfur....seems ok based on the def "The process in which a uridine residue at position 34 in the anticodon of a tRNA is post-transcriptionally thiolated at the C2 position. **This process involves transfer of a sulfur from cysteine to position C2 by several steps" ------- COMMENT: 736ae506059f399d 7 max4HEZNUxQSXwmzYVBOGtNvtHw (comment: CONDITION 25 degrees) ------- COMMENT: 736ae506059f399d 18 ewfspod7Bin5Lx5/B/NV8EarpfI (comment: CONDITION 30 degrees) ------- COMMENT: 736ae506059f399d 19 ewfspod7Bin5Lx5/B/NV8EarpfI (comment: CONDITION 30 degrees) ------- COMMENT: 736ae506059f399d 37 max4HEZNUxQSXwmzYVBOGtNvtHw (comment: CONDITION 25 degrees) ------- COMMENT: 736ae506059f399d 38 max4HEZNUxQSXwmzYVBOGtNvtHw (comment: CONDITION 25 degrees) ------- COMMENT: 738fa42d26db17a4 1 fk5kvD73MHrhVm/Vb8ULpgRlVGs Precise observation revealed that Mei2p dots could be visible in conjugating cells that completed cell fusion but did not undergo karyogamy yet. ------- COMMENT: 739c64f329b534e9 2 i0iVG0rAHq1pk1NFUzMWKWOFOP4 (comment: increased 25S/18S ratio) ------- COMMENT: 739f6a8893d0f034 3 RYwJvHuXVyagSc1DZG+WOCm2L+Y Diffuse cytoplasmic localisation at 37°C, no stress granules ------- COMMENT: 739f6a8893d0f034 4 hH7eANEO5wdjj1Sv8hETssZkc0Y Nuclear localization at 30°C ------- COMMENT: 739f6a8893d0f034 5 v7u6P6bxBM5PFfbtcA7NTXyWCvs Dcr1 localizes in electron-dense cytoplasmic inclusions at 37°C together with hsp104. Hsp104 is required for dissolution of these inclusions. ------- COMMENT: 739f6a8893d0f034 6 hH7eANEO5wdjj1Sv8hETssZkc0Y Nuclear localization at 30°C ------- COMMENT: 739f6a8893d0f034 7 O88GjjvmmRKttC9uFQK9gAgqALU Cytoplasmic localisation in electron-dense inclusions at 37°C ------- COMMENT: 739f6a8893d0f034 8 Z5K6ZeCvQ/EYwK/tQr+umWh1fVY hsp104 refolds dicer and is required for robust centromeric silencing at 37°C ------- COMMENT: 739f6a8893d0f034 9 Gtq4JrG/7lL2Kpa8ZHgf/LWrUFE Dcr1 represses hsp104 levels ------- COMMENT: 739f6a8893d0f034 12 1Cvj/4GOwD4BEebjhlfRapQEXQs A prionogenic reporter (S. cerevisiae Sup35 prion domain) aggregates in cytoplasmic inclusions in dcr1Δ ------- COMMENT: 739f6a8893d0f034 13 TYELeKAyCeP7LMBg+hNQNmBX+/A Dcr1 is not released from cytoplasmic inclusions at 37°C in hsp104Δ ------- COMMENT: 744e598799f5d648 1 7Fdz9/SaMaQl+X2bYOXm2W/kMOo Figure 4BD ------- COMMENT: 744e598799f5d648 2 7Fdz9/SaMaQl+X2bYOXm2W/kMOo Figure 4BD ------- COMMENT: 744e598799f5d648 3 x2kbXL/EWEreLGyc0rlf2m1SsE0 Figure 4BD, Figure 6BDE ------- COMMENT: 744e598799f5d648 4 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: 744e598799f5d648 5 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: 744e598799f5d648 6 FK4ODdotBEYO6vZBCDH14ZqONAk Figure 6BDE, Figure S2 ------- COMMENT: 744e598799f5d648 7 4+v0WjX0Tmo45YsFUgwtj/JJ6/k Figure 3AD, Figure 6ACE ------- COMMENT: 744e598799f5d648 8 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 744e598799f5d648 9 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 744e598799f5d648 10 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 744e598799f5d648 11 VRsYn0OmtRjt43iqVSLrELz97X4 Figure 5AB ------- COMMENT: 744e598799f5d648 12 VRsYn0OmtRjt43iqVSLrELz97X4 Figure 5AB ------- COMMENT: 744e598799f5d648 14 grYGNI/AtshVlAB8lyel78b+dj0 Figure 5AB, Figure S3 ------- COMMENT: 744e598799f5d648 15 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 744e598799f5d648 16 lgFe0LkcUT7B5dixpalwBKCJDOg Figure 3BE, Figure 6ACE ------- COMMENT: 744e598799f5d648 19 PBhF5yc5VS6q2Ab258bpo79RAQk Figure 4AC ------- COMMENT: 744e598799f5d648 20 PBhF5yc5VS6q2Ab258bpo79RAQk Figure 4AC ------- COMMENT: 744e598799f5d648 21 zRPiQ7B9o3MlO9NEUd+1WOESSX8 Figure 4AC, Figure 6BDE ------- COMMENT: 744e598799f5d648 22 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 744e598799f5d648 23 yjMlYrFKWVakNVO2vBWky7uhzg0 Figure 3CF, Figure 6ACE ------- COMMENT: 744e598799f5d648 24 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: 7482369b55e6cbf6 44 Z57aSDM+NQf54FiXEbhg73IC02E (comment: Can't say if they are viable vegetative because it is in a pyp2+ background) ------- COMMENT: 74dade3ace393390 9 E3iTbnZaDWigmAMnDAM6oET8r+4 Figure 1C ------- COMMENT: 74dade3ace393390 10 E3iTbnZaDWigmAMnDAM6oET8r+4 Figure 1C ------- COMMENT: 74dade3ace393390 14 Z5jbDMnRnf8OxN3NI9VAYy17wWk (fig 2) ------- COMMENT: 74dade3ace393390 15 Z5jbDMnRnf8OxN3NI9VAYy17wWk (fig 2) ------- COMMENT: 74dade3ace393390 16 z3LJO1Us83Ht/0UEJ+YP0eVilLg (Figure 2B) (comment: dependent on pds5) ------- COMMENT: 74dade3ace393390 17 hVBF0JlxBq71UOcxW8PFL8HBUQ0 (Figure 2B) ------- COMMENT: 74dade3ace393390 24 4oom2exDFoA4S+FHUoWDrlZek30 figure 3B ------- COMMENT: 74dade3ace393390 25 4oom2exDFoA4S+FHUoWDrlZek30 figure 3B ------- COMMENT: 74dade3ace393390 26 8F8k7RN2g6ngxsyw43muHmOOOzA figure 3B ------- COMMENT: 74dade3ace393390 27 8F8k7RN2g6ngxsyw43muHmOOOzA figure 3B ------- COMMENT: 74dade3ace393390 28 8F8k7RN2g6ngxsyw43muHmOOOzA figure 3B ------- COMMENT: 74dade3ace393390 32 C9q+H4ghbn4lvQCiMc6bjw1KPOE (Figure S4C) ------- COMMENT: 74dade3ace393390 35 lJWh/oU8xch24KKtvOsrodMwICU (Figure 6A) ------- COMMENT: 74dade3ace393390 36 lJWh/oU8xch24KKtvOsrodMwICU (Figure 6A) ------- COMMENT: 74dade3ace393390 37 aw+aavyuaA4vcKjC6tXGX3OI+Wo (Figure 6B) (comment: CHECK acetylated form acts as a DNA sensor) ------- COMMENT: 74dade3ace393390 38 AqR6pEt4KJ+lmaI+kSmhguV++co (Figure 6C) ------- COMMENT: 74e623dba5700603 1 zv+Er/ofd76SqcPDc1Z/2M//LuU (comment: CHECK inhibited_by L-lysine) ------- COMMENT: 7519fc48320b66a8 43 AtrUe1slx2j9qzn+tLxyzuPD+PQ (comment: there is good evidence for this, but not bullet proof) ------- COMMENT: 7537db502e936856 51 e3I1HSA6aGnEFj6ge9oWTxX7ODo (comment: they interacted in the Y2H experiment, so inferring this relationship) ------- COMMENT: 755324957441e428 5 RsdC3K4j13cvuRV/00rvdOEDtA4 (comment: CHECK level of mutant cdc18deltaCDK1-5 protein) ------- COMMENT: 7574a76221b0ed26 1 yf317FUsOstAy4uaXajU9oB5Rpo Fig. 1D-E ------- COMMENT: 7574a76221b0ed26 2 IlJQhTk5Ek+O0t0PZFSi2MjRYIU (Fig. 1F-G ------- COMMENT: 7574a76221b0ed26 4 OA199IQrhjShATw4GPoko6BalJw (comment: interaction mediated by Opy1 PH1 domain (aa1-128)) ------- COMMENT: 7574a76221b0ed26 5 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 7574a76221b0ed26 6 Nc4FYEwdG13iU1xH2pi7gNFT0yk Figure 4E-F ------- COMMENT: 7574a76221b0ed26 7 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 7574a76221b0ed26 8 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 7574a76221b0ed26 9 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 7574a76221b0ed26 10 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 7574a76221b0ed26 11 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: 7574a76221b0ed26 12 +kJuYLVQKW4u1qSTgb27mCD/s28 (Fig. S2B) ------- COMMENT: 7574a76221b0ed26 13 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: 7574a76221b0ed26 14 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: 7574a76221b0ed26 15 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: 7574a76221b0ed26 16 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: 7574a76221b0ed26 17 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: 7574a76221b0ed26 18 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 7574a76221b0ed26 19 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 7574a76221b0ed26 20 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 7574a76221b0ed26 22 xsdX+hxUbzv5fc2e22poy+uapk8 (comment: Opy1 PH1 (aa1-128) can directly bind phospholipids in vitro) Figure 1 ------- COMMENT: 7574a76221b0ed26 23 HpjBOwbW9DLVclETqgy3unfNjBs (comment: I changed the function annotation to this process annotation because it precisely negates the SGD annotation) ------- COMMENT: 7574a76221b0ed26 24 r/STLct0ZJ9EU6sKtRhTJIa4THE Figure 1C (comment: CHECK requested normal membrane lipid binding) ------- COMMENT: 7574a76221b0ed26 25 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 7574a76221b0ed26 26 DtRGuVj8SrYIDby9VfdnkRg+dAo (comment: PH1 domain) Fig. 2A, Table S2 ------- COMMENT: 7574a76221b0ed26 27 jCcQrGqXlX8lxKCdySPBpGO8l+s (comment: CHECK THIS IS A GUESS I COULD NOT ACCESS THE SUPP SO ANNOTATED TO THE Snider ITS3-1 growth phenotype) ------- COMMENT: 7574a76221b0ed26 30 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: 7574a76221b0ed26 31 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: 7574a76221b0ed26 33 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: 7574a76221b0ed26 34 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: 7574a76221b0ed26 35 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: 7574a76221b0ed26 36 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 758c93b03dfe5c59 47 QTgQAFXn5vHPyWbzktB9dHcCkGM (comment: CHECK more specifically, response to mitotic DNA replication checkpoint signaling) ------- COMMENT: 75dadd0ae174dd58 18 0Sds6XG2TsR7xUvmOXE51Ep+MIU (comment: Proxy assay for hydrolase function used and IMP evidence for catalytic activity) ------- COMMENT: 75f4f061893fee16 2 NWoZK3kTsExUV00Ywo1G5jlUKKs Figure 1 ------- COMMENT: 75f4f061893fee16 4 LZGiCiD8rrCuYLUYm4EL34SBsdc Figure 1b ------- COMMENT: 75f4f061893fee16 5 LZGiCiD8rrCuYLUYm4EL34SBsdc Figure 1b ------- COMMENT: 75f4f061893fee16 6 LZGiCiD8rrCuYLUYm4EL34SBsdc Figure 1b ------- COMMENT: 75f4f061893fee16 7 LZGiCiD8rrCuYLUYm4EL34SBsdc Figure 1b ------- COMMENT: 75f4f061893fee16 8 LZGiCiD8rrCuYLUYm4EL34SBsdc Figure 1b ------- COMMENT: 75f4f061893fee16 9 LZGiCiD8rrCuYLUYm4EL34SBsdc Figure 1b ------- COMMENT: 75f4f061893fee16 10 LZGiCiD8rrCuYLUYm4EL34SBsdc Figure 1b ------- COMMENT: 75f4f061893fee16 11 LZGiCiD8rrCuYLUYm4EL34SBsdc Figure 1b ------- COMMENT: 75f4f061893fee16 12 LZGiCiD8rrCuYLUYm4EL34SBsdc Figure 1b ------- COMMENT: 75f4f061893fee16 13 LZGiCiD8rrCuYLUYm4EL34SBsdc Figure 1b ------- COMMENT: 75f4f061893fee16 14 LZGiCiD8rrCuYLUYm4EL34SBsdc Figure 1b ------- COMMENT: 75f4f061893fee16 15 LZGiCiD8rrCuYLUYm4EL34SBsdc Figure 1b ------- COMMENT: 75f4f061893fee16 16 HBL6UR1SGUpWge6L5BoeOY2F8pA Figure S1 ------- COMMENT: 75f4f061893fee16 17 CFnQxPG6p9ywfLGuxKEmPsOSKeQ Figure 1e ------- COMMENT: 75f4f061893fee16 18 CFnQxPG6p9ywfLGuxKEmPsOSKeQ Figure 1e ------- COMMENT: 75f4f061893fee16 20 vTiQ0qPlitMtMEsoxrALoD2tXpE Figure 1f ------- COMMENT: 75f4f061893fee16 22 2kuSN7rMzfGcB2DKt67EqDWQELA Figure 2 ------- COMMENT: 75f4f061893fee16 23 2kuSN7rMzfGcB2DKt67EqDWQELA Figure 2 ------- COMMENT: 75f4f061893fee16 24 2kuSN7rMzfGcB2DKt67EqDWQELA Figure 2 ------- COMMENT: 75f4f061893fee16 25 2kuSN7rMzfGcB2DKt67EqDWQELA Figure 2 ------- COMMENT: 75f4f061893fee16 26 2kuSN7rMzfGcB2DKt67EqDWQELA Figure 2 ------- COMMENT: 75f4f061893fee16 27 2kuSN7rMzfGcB2DKt67EqDWQELA Figure 2 ------- COMMENT: 75f4f061893fee16 28 iDRq5uBjot6W0MbUCGD0VhIyaGM Figure 2f ------- COMMENT: 75f4f061893fee16 29 iDRq5uBjot6W0MbUCGD0VhIyaGM Figure 2f ------- COMMENT: 75f4f061893fee16 30 iDRq5uBjot6W0MbUCGD0VhIyaGM Figure 2f ------- COMMENT: 75f4f061893fee16 31 iDRq5uBjot6W0MbUCGD0VhIyaGM Figure 2f ------- COMMENT: 75f4f061893fee16 34 QgVxTN/hTtnj0DDd94h3gblk9RA Figure S2 ------- COMMENT: 75f4f061893fee16 35 QgVxTN/hTtnj0DDd94h3gblk9RA Figure S2 ------- COMMENT: 75f4f061893fee16 40 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 75f4f061893fee16 47 G2RTiSRzpGfQc3LUXrBavCAxZHo Figure 4 ------- COMMENT: 75f4f061893fee16 48 rDR41po8gfpi5g9cNpYWWk5easQ Figure 5 ------- COMMENT: 75f4f061893fee16 49 rDR41po8gfpi5g9cNpYWWk5easQ Figure 5 ------- COMMENT: 75f4f061893fee16 50 rDR41po8gfpi5g9cNpYWWk5easQ Figure 5 ------- COMMENT: 75f4f061893fee16 51 wd/ZbuqMwrYnhSdbyjisJhJW4ng Figure 6 ------- COMMENT: 75f4f061893fee16 52 wd/ZbuqMwrYnhSdbyjisJhJW4ng Figure 6 ------- COMMENT: 75f4f061893fee16 53 wd/ZbuqMwrYnhSdbyjisJhJW4ng Figure 6 ------- COMMENT: 75f4f061893fee16 54 wd/ZbuqMwrYnhSdbyjisJhJW4ng Figure 6 ------- COMMENT: 75f4f061893fee16 55 wd/ZbuqMwrYnhSdbyjisJhJW4ng Figure 6 ------- COMMENT: 75f4f061893fee16 56 wd/ZbuqMwrYnhSdbyjisJhJW4ng Figure 6 ------- COMMENT: 75f4f061893fee16 57 F62KvGgnp7fbYopogjhGB4ixHow Figure 6b ------- COMMENT: 75f4f061893fee16 58 wd/ZbuqMwrYnhSdbyjisJhJW4ng Figure 6 ------- COMMENT: 75f4f061893fee16 59 wd/ZbuqMwrYnhSdbyjisJhJW4ng Figure 6 ------- COMMENT: 75f4f061893fee16 60 wd/ZbuqMwrYnhSdbyjisJhJW4ng Figure 6 ------- COMMENT: 75f4f061893fee16 61 kCujzaGIOAFZS24bRSeQzFOUj9o Figure 7 ------- COMMENT: 75f4f061893fee16 62 kCujzaGIOAFZS24bRSeQzFOUj9o Figure 7 ------- COMMENT: 75f4f061893fee16 63 kCujzaGIOAFZS24bRSeQzFOUj9o Figure 7 ------- COMMENT: 75f4f061893fee16 64 kCujzaGIOAFZS24bRSeQzFOUj9o Figure 7 ------- COMMENT: 75f4f061893fee16 65 kCujzaGIOAFZS24bRSeQzFOUj9o Figure 7 ------- COMMENT: 75f4f061893fee16 66 kCujzaGIOAFZS24bRSeQzFOUj9o Figure 7 ------- COMMENT: 75f4f061893fee16 67 k2MK3IUMcXyWGFrlmUBwSpNgZpE Figure 7, (comment: type II cells) ------- COMMENT: 760045d7bb2de1aa 3 TFpCqcnaxhracPZXtqhXNsY2Hkk Previous work demonstrated that Rho2 GTPase, one of the six Rho GTPases found in S. pombe proteome (Rho1 to Rho5, and Cdc42) which controls cell polarity and cell wall biosynthesis, is a positive regulator operating upstream of the CIP [13,14] ------- COMMENT: 760045d7bb2de1aa 4 gt7EK2yblQV/DFaRYekl2/wrRes In this study we show for the first time that Rho1 and Pck1 are true activators of this signalling cascade in addition to Rho2 and Pck2 under specific environmental contexts ------- COMMENT: 760045d7bb2de1aa 5 gt7EK2yblQV/DFaRYekl2/wrRes In this study we show for the first time that Rho1 and Pck1 are true activators of this signalling cascade in addition to Rho2 and Pck2 under specific environmental contexts ------- COMMENT: 760045d7bb2de1aa 7 ygmbqarD9+fzQDAt3JS6mQUfo6I Also, Pmk1 hyperactivation triggered by rho2+ overexpression is fully attenuated in mutants lacking Pck2 (Figure 1A). ------- COMMENT: 760045d7bb2de1aa 8 ygmbqarD9+fzQDAt3JS6mQUfo6I Also, Pmk1 hyperactivation triggered by rho2+ overexpression is fully attenuated in mutants lacking Pck2 (Figure 1A). ------- COMMENT: 760045d7bb2de1aa 9 5vMgi76mQ0/FNCbxHeZ5G2J9vPM Taken as a whole, these results support that Pck1 might act as a Rho1 target during signal transmission to the CIP, although its role within this pathway seems restricted to specific situations. ------- COMMENT: 760045d7bb2de1aa 10 jSKBtkVrUgDMjlN6/dRWMoptqGQ Rho1 GTPase might modulate the activity Pmk1 by acting upstream of Pck2 because it has been described that overexpres- sion of wild type or a constitutively active allele of rho1+ (G15V mutant) induced a marked hyperactivation of Pmk1 (Figure 1B ------- COMMENT: 760045d7bb2de1aa 11 rI0ufQN5SQzSFn4ihBkcRCkJ0GA However, a careful examination of these experiments revealed that Pmk1 basal phosphorylation in rho1-596 rho2D cells was actually lower than in rho2D cells, and this difference was statistically significant (P,0.04; Figure 1C). On the contrary, basal Pmk1 activity was nearly identical in pck2D and rho1-596 pck2D cells (Figure 1D), strongly suggesting that enhanced Pmk1 activation in rho1-596 cells is transmitted to the MAPK cascade mainly through Pck2. ------- COMMENT: 760045d7bb2de1aa 12 qT5gKD3TsE7BkbGepml5/vGUf9E As seen in Figure 1E, rho2D cells showed a partial VIC phenotype in medium supplemented with 0.2 M MgCl2 and became VIC negative in the presence of 0.3 M MgCl2. ------- COMMENT: 760045d7bb2de1aa 13 qT5gKD3TsE7BkbGepml5/vGUf9E As seen in Figure 1E, rho2D cells showed a partial VIC phenotype in medium supplemented with 0.2 M MgCl2 and became VIC negative in the presence of 0.3 M MgCl2. ------- COMMENT: 760045d7bb2de1aa 14 r4V8ZanDCWdx1bMa4K17DN2mJl4 As expected, both wild type and rho1- 596 cells were VIC negative under any condition (Figure 1D). ------- COMMENT: 760045d7bb2de1aa 15 KZuUWbTZbdk1gC/yLgAY99pgHCg Importantly, the VIC phenotype in rho1-596 rho2D double mutant was markedly enhanced as compared to that shown by rho2D cells (Figure 1E), which is in good agreement with basal Pmk1 phosphorylation data (Figure 1C). ------- COMMENT: 760045d7bb2de1aa 16 XneQ3Twek7SjYGg2oUZhvN8kaNo As a whole, these results sustain that Rho1 GTPase is a true positive regulator of the cell integrity pathway which operates during vegetative growth in an alternative fashion to Rho2 and using Pck2 as a main target. ------- COMMENT: 760045d7bb2de1aa 17 zfLzoZChiNcxLLHj/KxAKxXwZRE Pmk1 activation induced by hypo- and hyper-osmotic stress totally depends upon the signaling mediated by Rho2 (Figure 2A) ------- COMMENT: 760045d7bb2de1aa 18 sfWNV6/W00fxSZ5grwzd/02nfrA (comment: CHECK in response to oxidative stress) On the contrary, MAPK activation triggered by oxidative (hydrogen peroxide) and cell wall (Caspofungin) stresses is only partially dependent on this GTPase (Figure 2B and C) ------- COMMENT: 760045d7bb2de1aa 19 3FCB0YW5TcuRI+nWurVwHRHF9fs (comment: CHECK in response to oxidative stress) As shown in Figure 2B, Pmk1 activation in rho2D cells subjected to oxidative stress was not affected by simultaneous expression of the rho1-596 hypoactive allele. ------- COMMENT: 760045d7bb2de1aa 20 lj6X/kSxEQp+JOv0HiJ7gY4Kxu8 However, in comparison to either control or rho2D and rho1-596 single mutant cells, MAPK activation was severely compromised in cells from the rho2D rho1-596 double mutant treated with Caspofungin (Figure 2C). ------- COMMENT: 760045d7bb2de1aa 21 aZbV5yPLPTGemCYVny44YFZTWaQ Figure 3A indicates that deletion of rho2+ gene alleviated the increased Pmk1 basal phosphorylation present in pck1D cells. ------- COMMENT: 760045d7bb2de1aa 22 aZbV5yPLPTGemCYVny44YFZTWaQ Figure 3A indicates that deletion of rho2+ gene alleviated the increased Pmk1 basal phosphorylation present in pck1D cells. ------- COMMENT: 760045d7bb2de1aa 23 aZbV5yPLPTGemCYVny44YFZTWaQ Figure 3A indicates that deletion of rho2+ gene alleviated the increased Pmk1 basal phosphorylation present in pck1D cells. ------- COMMENT: 760045d7bb2de1aa 24 AHqB6GzFmDDdrzGU2hAIwo95/sg (comment: note in Figure 3B the robust growth of rho1-596 rho2D pck1D cells in medium supplemented with 0.2 M MgCl2 as compared to rho2D pck1D cells). ------- COMMENT: 760045d7bb2de1aa 25 hj6EXXvZ1eG8nSqKWlqMczH6aVY Notably, as shown Figure 3F, we detected the Mkh1-myc fusion after Pck1 immunoprecip`ıtation only in Caspofungin-treated pck2D cells, but not in growing cells or strains expressing Pck2. ------- COMMENT: 760d48a1560efbfc 8 RW9kpTbxr70cmlIRnX2ybOCGZlM (comment: CHECK high temp is permissive) ------- COMMENT: 760d48a1560efbfc 10 S+PWCwRFSMYvKUQTFqIrJgdtGDQ (comment: CHECK standard temp is restrictive) ------- COMMENT: 760d48a1560efbfc 22 RW9kpTbxr70cmlIRnX2ybOCGZlM (comment: CHECK high temp is permissive) ------- COMMENT: 760d48a1560efbfc 27 RW9kpTbxr70cmlIRnX2ybOCGZlM (comment: CHECK high temp is permissive) ------- COMMENT: 760d48a1560efbfc 34 2+alL/PLlxRpgLmUVA+imGLI38w comment: CHECK temperature restrictive for dis2cs alone) ------- COMMENT: 760d48a1560efbfc 35 bh3p6+rVtk7S5WiPt+fd9YIgrHQ comment: CHECK temperature restrictive for dis2cs alone) ------- COMMENT: 760d48a1560efbfc 40 2+alL/PLlxRpgLmUVA+imGLI38w comment: CHECK temperature restrictive for dis2cs alone) ------- COMMENT: 760d48a1560efbfc 41 2+alL/PLlxRpgLmUVA+imGLI38w comment: CHECK temperature restrictive for dis2cs alone) ------- COMMENT: 760d48a1560efbfc 42 2+alL/PLlxRpgLmUVA+imGLI38w comment: CHECK temperature restrictive for dis2cs alone) ------- COMMENT: 760d48a1560efbfc 43 2+alL/PLlxRpgLmUVA+imGLI38w comment: CHECK temperature restrictive for dis2cs alone) ------- COMMENT: 7622b54e59b561d0 5 8Ng01IkMamC5e7yxjEIeIIidD8I (comment: residues include one or more of S77, T78, T79, S87, and T89, and others) ------- COMMENT: 7622b54e59b561d0 81 1FFFMhEmQWokGtjb9DP5VDMgZ7w (comment: ctp-Phosphorylated) ------- COMMENT: 76625df886d53d47 1 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 76625df886d53d47 2 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 76625df886d53d47 7 jAWL+XgsuEzdACcFWh1U9YqTd7s we observed Cdc42 activation in early anaphase, at the time of ring assembly. Fig. 2 ------- COMMENT: 76625df886d53d47 8 a4y8z2wG3lSlNCfG5QVMi1DmwAw we found that the Rho probe RBD–tdTomato localized to the division site in late anaphase, immediately preceding the onset of ring constriction and septum ingression. Fig. 2 ------- COMMENT: 76625df886d53d47 9 oSUoPHzYlm8X08d2UX8mTPNMSD8 we found that in most cells, Rgf1– GFP and Rgf3–eGFP was localized to the division site at early stages in anaphase (Fig. S3D,E). ------- COMMENT: 76625df886d53d47 10 oSUoPHzYlm8X08d2UX8mTPNMSD8 we found that in most cells, Rgf1– GFP and Rgf3–eGFP was localized to the division site at early stages in anaphase (Fig. S3D,E). ------- COMMENT: 76625df886d53d47 11 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 76625df886d53d47 12 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 76625df886d53d47 13 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 76625df886d53d47 14 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 76625df886d53d47 15 lcOMVTa4EHt0FNqnoS9y+DS5qao However, early RBD–mNG localization at the division site observed in gef1Δ cells was rescued upon pak1OE, restoring it to late anaphase (Fig. S6A, B). ------- COMMENT: 76625df886d53d47 16 C7VXIe4xnEBJZFh+t5OmOTsfZkg Fig. S7D ------- COMMENT: 76625df886d53d47 17 RmaypUGy3x3DjMhTAhkf8bEgGN4 Fig. S7C ------- COMMENT: 76625df886d53d47 18 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 76625df886d53d47 19 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 76625df886d53d47 20 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 76625df886d53d47 21 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 76625df886d53d47 22 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: 76625df886d53d47 23 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: 76625df886d53d47 24 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 76625df886d53d47 25 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 76625df886d53d47 26 xLld+U4XtO1Yq5XJ/vA+lPgihjU Fig. 6D ------- COMMENT: 76625df886d53d47 27 xLld+U4XtO1Yq5XJ/vA+lPgihjU Fig. 6D ------- COMMENT: 76625df886d53d47 28 q3+3BbedTFTMvhIMxAYDGxfKWwQ Fig. 6D and Fig. S7F ------- COMMENT: 76625df886d53d47 29 q3+3BbedTFTMvhIMxAYDGxfKWwQ Fig. 6D and Fig. S7F ------- COMMENT: 76625df886d53d47 30 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 76625df886d53d47 31 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 76625df886d53d47 32 MaAy50huyr2Z8YhZcJ6Dm4BZBgE Fig. S4A ------- COMMENT: 76625df886d53d47 33 MaAy50huyr2Z8YhZcJ6Dm4BZBgE Fig. S4A ------- COMMENT: 76625df886d53d47 34 MaAy50huyr2Z8YhZcJ6Dm4BZBgE Fig. S4A ------- COMMENT: 76625df886d53d47 35 MaAy50huyr2Z8YhZcJ6Dm4BZBgE Fig. S4A ------- COMMENT: 767451d8f8ef6abe 1 Pb4tXoKNPLvHK9ZZz1ZL8MBYriY (comment: CHECK i) unknown kinase ii) asynchronous cells iii) unknown) ------- COMMENT: 767451d8f8ef6abe 2 wCY3eXpUUP0RFgEAKLlRaOcxKss (comment: CHECKi) unknown kinase ii) asynchronous cells iii) unknown) ------- COMMENT: 767451d8f8ef6abe 4 wCY3eXpUUP0RFgEAKLlRaOcxKss (comment: CHECK i) unknown kinase ii) asynchronous cells iii) unknown) ------- COMMENT: 767451d8f8ef6abe 5 Pb4tXoKNPLvHK9ZZz1ZL8MBYriY (comment: CHECK i) unknown kinase ii) asynchronous cells iii) unknown) ------- COMMENT: 767451d8f8ef6abe 6 wCY3eXpUUP0RFgEAKLlRaOcxKss (comment: CHECK i) unknown kinase ii) asynchronous cells iii) unknown) ------- COMMENT: 767451d8f8ef6abe 7 Pb4tXoKNPLvHK9ZZz1ZL8MBYriY (comment: CHECK i) unknown kinase ii) asynchronous cells iii) unknown) ------- COMMENT: 767451d8f8ef6abe 8 Pb4tXoKNPLvHK9ZZz1ZL8MBYriY (comment: CHECK i) unknown kinase ii) asynchronous cells iii) unknown) ------- COMMENT: 767451d8f8ef6abe 9 Pb4tXoKNPLvHK9ZZz1ZL8MBYriY (comment: CHECK i) unknown kinase ii) asynchronous cells iii) unknown) ------- COMMENT: 767451d8f8ef6abe 10 Pb4tXoKNPLvHK9ZZz1ZL8MBYriY (comment: CHECK i) unknown kinase ii) asynchronous cells iii) unknown) ------- COMMENT: 767451d8f8ef6abe 11 Pb4tXoKNPLvHK9ZZz1ZL8MBYriY (comment: CHECK i) unknown kinase ii) asynchronous cells iii) unknown) ------- COMMENT: 767451d8f8ef6abe 12 wCY3eXpUUP0RFgEAKLlRaOcxKss (comment: CHECK i) unknown kinase ii) asynchronous cells iii) unknown) ------- COMMENT: 767451d8f8ef6abe 13 Pb4tXoKNPLvHK9ZZz1ZL8MBYriY (comment: CHECK i) unknown kinase ii) asynchronous cells iii) unknown) ------- COMMENT: 767451d8f8ef6abe 14 Pb4tXoKNPLvHK9ZZz1ZL8MBYriY (comment: CHECK i) unknown kinase ii) asynchronous cells iii) unknown) ------- COMMENT: 767451d8f8ef6abe 15 Pb4tXoKNPLvHK9ZZz1ZL8MBYriY (comment: CHECK i) unknown kinase ii) asynchronous cells iii) unknown) ------- COMMENT: 767451d8f8ef6abe 16 Pb4tXoKNPLvHK9ZZz1ZL8MBYriY (comment: CHECK i) unknown kinase ii) asynchronous cells iii) unknown) ------- COMMENT: 767451d8f8ef6abe 17 Pb4tXoKNPLvHK9ZZz1ZL8MBYriY (comment: CHECK i) unknown kinase ii) asynchronous cells iii) unknown) ------- COMMENT: 767451d8f8ef6abe 18 Pb4tXoKNPLvHK9ZZz1ZL8MBYriY (comment: CHECK i) unknown kinase ii) asynchronous cells iii) unknown) ------- COMMENT: 767451d8f8ef6abe 19 wCY3eXpUUP0RFgEAKLlRaOcxKss (comment: CHECK i) unknown kinase ii) asynchronous cells iii) unknown) ------- COMMENT: 767451d8f8ef6abe 20 wCY3eXpUUP0RFgEAKLlRaOcxKss (comment: CHECK i) unknown kinase ii) asynchronous cells iii) unknown) ------- COMMENT: 767451d8f8ef6abe 21 Pb4tXoKNPLvHK9ZZz1ZL8MBYriY (comment: CHECK i) unknown kinase ii) asynchronous cells iii) unknown) ------- COMMENT: 767451d8f8ef6abe 22 wCY3eXpUUP0RFgEAKLlRaOcxKss (comment: CHECK i) unknown kinase ii) asynchronous cells iii) unknown) ------- COMMENT: 76a3cc41b9c66144 16 OVl8mbEx2lEW7GhurI0Z0Bb6WIA ------- COMMENT: 76a3cc41b9c66144 17 OVl8mbEx2lEW7GhurI0Z0Bb6WIA ------- COMMENT: 76a3cc41b9c66144 23 FtBZwrZnNMAv7cU3+qzs+elOxy4 (comment: same as cdc20-M10 alone) ------- COMMENT: 76a3cc41b9c66144 26 OVl8mbEx2lEW7GhurI0Z0Bb6WIA ------- COMMENT: 76a3cc41b9c66144 27 OVl8mbEx2lEW7GhurI0Z0Bb6WIA ------- COMMENT: 76dce1150495eda7 1 dSuM4+IyDolwEuKsWneeLfc4zg4 (Fig. 1B and C) ------- COMMENT: 76dce1150495eda7 2 dSuM4+IyDolwEuKsWneeLfc4zg4 (Fig. 1B and C) ------- COMMENT: 76dce1150495eda7 3 dSuM4+IyDolwEuKsWneeLfc4zg4 (Fig. 1B and C) ------- COMMENT: 76dce1150495eda7 4 dSuM4+IyDolwEuKsWneeLfc4zg4 (Fig. 1B and C) ------- COMMENT: 76dce1150495eda7 5 dSuM4+IyDolwEuKsWneeLfc4zg4 (Fig. 1B and C) ------- COMMENT: 76dce1150495eda7 6 dSuM4+IyDolwEuKsWneeLfc4zg4 (Fig. 1B and C) ------- COMMENT: 76dce1150495eda7 7 dSuM4+IyDolwEuKsWneeLfc4zg4 (Fig. 1B and C) ------- COMMENT: 76dce1150495eda7 8 dSuM4+IyDolwEuKsWneeLfc4zg4 (Fig. 1B and C) ------- COMMENT: 76dce1150495eda7 9 dSuM4+IyDolwEuKsWneeLfc4zg4 (Fig. 1B and C) ------- COMMENT: 76dce1150495eda7 10 dSuM4+IyDolwEuKsWneeLfc4zg4 (Fig. 1B and C) ------- COMMENT: 76dce1150495eda7 11 dSuM4+IyDolwEuKsWneeLfc4zg4 (Fig. 1B and C) ------- COMMENT: 76dce1150495eda7 12 dSuM4+IyDolwEuKsWneeLfc4zg4 (Fig. 1B and C) ------- COMMENT: 76dce1150495eda7 13 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A and B) ------- COMMENT: 76dce1150495eda7 14 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A and B) ------- COMMENT: 76dce1150495eda7 15 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A and B) ------- COMMENT: 76dce1150495eda7 16 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: 76dce1150495eda7 17 ojGoCV7tkFuQq8EQLxws0hw4rK4 (Fig. S2D) ------- COMMENT: 76dce1150495eda7 18 aKI0ZBbuMtzcCHGSKLtz2gPBU6E Cid12 had robust adenylation activity when assembled into the RDRC complex, containing either wild-type Rdp1 or catalytically inac- tive Rdp1D903A (Figure 2G, lanes 1, 3, and 4), suggesting that Cid12 activity was allosterically regulated. ------- COMMENT: 76dce1150495eda7 19 5a7fvmcTov3dOR2/Er4Wu3j4EM0 (Fig. 3A and B) ------- COMMENT: 76dce1150495eda7 20 5a7fvmcTov3dOR2/Er4Wu3j4EM0 (Fig. 3A and B) ------- COMMENT: 76dce1150495eda7 21 5a7fvmcTov3dOR2/Er4Wu3j4EM0 (Fig. 3A and B) ------- COMMENT: 76dce1150495eda7 22 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 76dce1150495eda7 23 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 76dce1150495eda7 24 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 76dce1150495eda7 25 y7Yq/bA6j8EwABo1e7r6dVZsuN8 (Fig. 4I) ------- COMMENT: 76dce1150495eda7 26 y7Yq/bA6j8EwABo1e7r6dVZsuN8 (Fig. 4I) ------- COMMENT: 76dce1150495eda7 27 9SfVXX3Z0+oPWGxDwp4evI86bGw (Fig. 5 and Fig. S5) ------- COMMENT: 76dce1150495eda7 28 9SfVXX3Z0+oPWGxDwp4evI86bGw (Fig. 5 and Fig. S5) ------- COMMENT: 76dce1150495eda7 29 9SfVXX3Z0+oPWGxDwp4evI86bGw (Fig. 5 and Fig. S5) ------- COMMENT: 76dce1150495eda7 30 SlilJ9LAT1PbyRW0Ha5OfgJITG0 (Fig. 5C and E) ------- COMMENT: 76dce1150495eda7 31 SlilJ9LAT1PbyRW0Ha5OfgJITG0 (Fig. 5C and E) ------- COMMENT: 76dce1150495eda7 32 SlilJ9LAT1PbyRW0Ha5OfgJITG0 (Fig. 5C and E) ------- COMMENT: 76dce1150495eda7 33 SlilJ9LAT1PbyRW0Ha5OfgJITG0 (Fig. 5C and E) ------- COMMENT: 76dce1150495eda7 34 DVJMM6AvJyLz8J8dkwtY+pFizxc (Fig. 5E) ------- COMMENT: 76dce1150495eda7 35 DVJMM6AvJyLz8J8dkwtY+pFizxc (Fig. 5E) ------- COMMENT: 76dce1150495eda7 36 qUSVfuyqqt9F2ec/EoCkJxpjOWY (Fig. 5G and Fig. S5B) ------- COMMENT: 76dce1150495eda7 37 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: 76dce1150495eda7 38 DVJMM6AvJyLz8J8dkwtY+pFizxc (Fig. 5E) ------- COMMENT: 76dce1150495eda7 39 DVJMM6AvJyLz8J8dkwtY+pFizxc (Fig. 5E) ------- COMMENT: 76dce1150495eda7 40 FWjK3X3nwePZ2SJOx4aDNWjulKg (Fig. 6B, D and E) ------- COMMENT: 76dce1150495eda7 41 RqLSGZPI+V40IwJrnFT8AnPDyCo we provide evidence that siRNAs undergo processing at their 3'ends, which involves the addition of untemplated nucleotides by the Cid12 and Cid14 nucleotidyltransferases ------- COMMENT: 76dce1150495eda7 43 RqLSGZPI+V40IwJrnFT8AnPDyCo we provide evidence that siRNAs undergo processing at their 3'ends, which involves the addition of untemplated nucleotides by the Cid12 and Cid14 nucleotidyltransferases ------- COMMENT: 77007fae1e2ad4f9 1 ROeCx0JTXFQl/rfcZ7X2RPqZhp8 (comment: CHECK during mitotic DNA replication initiation) ------- COMMENT: 77007fae1e2ad4f9 2 4DZINgrMJu1M0ws/mhveAbL/q50 (comment: delete if superseded; authors not sure if it's just a detection issue, but they don't see Rad4 moving away from origins as Mcm10 does) ------- COMMENT: 77007fae1e2ad4f9 3 nyVpiP68jrO+F7vPOHgjt01L5pk (comment: also inferred from interaction with Cdc23 and from timing of localization to chromatin at origins) ------- COMMENT: 77007fae1e2ad4f9 7 ROeCx0JTXFQl/rfcZ7X2RPqZhp8 (comment: CHECK during mitotic DNA replication initiation) ------- COMMENT: 77007fae1e2ad4f9 8 ROeCx0JTXFQl/rfcZ7X2RPqZhp8 (comment: CHECK during mitotic DNA replication initiation) ------- COMMENT: 77007fae1e2ad4f9 9 ErMu9wbCD7k8xsOZJdhkc7RUUfk (comment: during replication fork processing) ------- COMMENT: 77007fae1e2ad4f9 10 ErMu9wbCD7k8xsOZJdhkc7RUUfk (comment: during replication fork processing) ------- COMMENT: 77007fae1e2ad4f9 11 ErMu9wbCD7k8xsOZJdhkc7RUUfk (comment: during replication fork processing) ------- COMMENT: 77007fae1e2ad4f9 12 ErMu9wbCD7k8xsOZJdhkc7RUUfk (comment: during replication fork processing) ------- COMMENT: 77214573d0c216fa 1 FbS+xiKKnMv289rZzBzE2CKOMPk ------- COMMENT: 772e462e74088043 2 vmEJiaZHWs8NOGPIR/b25oDdqHU Figure 6C ------- COMMENT: 772e462e74088043 3 vmEJiaZHWs8NOGPIR/b25oDdqHU Figure 6C ------- COMMENT: 772e462e74088043 4 /YkIe7CDyr/VR6iTzZSJbo1KYjA Figure 6c ------- COMMENT: 772e462e74088043 5 5Leuj0nQtQ/oH58v8XLkYXSDMog Figure 6B ------- COMMENT: 772e462e74088043 6 vmEJiaZHWs8NOGPIR/b25oDdqHU Figure 6C ------- COMMENT: 772e462e74088043 7 5Leuj0nQtQ/oH58v8XLkYXSDMog Figure 6B ------- COMMENT: 772e462e74088043 8 vmEJiaZHWs8NOGPIR/b25oDdqHU Figure 6C ------- COMMENT: 772e462e74088043 9 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: 772e462e74088043 10 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: 772e462e74088043 11 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: 772e462e74088043 12 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: 772e462e74088043 13 vmEJiaZHWs8NOGPIR/b25oDdqHU Figure 6C ------- COMMENT: 772e462e74088043 14 vmEJiaZHWs8NOGPIR/b25oDdqHU Figure 6C ------- COMMENT: 7744c021aa35dd69 32 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 7744c021aa35dd69 33 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 7744c021aa35dd69 34 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 7744c021aa35dd69 35 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 7744c021aa35dd69 47 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 7744c021aa35dd69 48 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 7744c021aa35dd69 49 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 7744c021aa35dd69 50 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 7744c021aa35dd69 52 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 7744c021aa35dd69 53 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 7744c021aa35dd69 54 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 7744c021aa35dd69 55 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 7744c021aa35dd69 57 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 7744c021aa35dd69 58 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 7744c021aa35dd69 59 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 7744c021aa35dd69 60 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 7744c021aa35dd69 62 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 7744c021aa35dd69 63 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 7744c021aa35dd69 64 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 7744c021aa35dd69 65 wgQVuyuEMLLI6mf8hAWnGN++Xvk (comment: no expressivity extension because of decreased growth when untreated) ------- COMMENT: 774b7f1a9f489629 39 UmjVdyxKy7qGZI9KQNTFRw2t9hU (comment: same as cdc25-22 single mutant) ------- COMMENT: 774b7f1a9f489629 40 UmjVdyxKy7qGZI9KQNTFRw2t9hU (comment: same as cdc25-22 single mutant) ------- COMMENT: 774b7f1a9f489629 41 UmjVdyxKy7qGZI9KQNTFRw2t9hU (comment: same as cdc25-22 single mutant) ------- COMMENT: 774b7f1a9f489629 42 UmjVdyxKy7qGZI9KQNTFRw2t9hU (comment: same as cdc25-22 single mutant) ------- COMMENT: 7777e3bab77e1283 1 icgHlILYf0hjbhQtO4OUh/HO+6w (comment: Promoter analysis) ------- COMMENT: 7777e3bab77e1283 2 icgHlILYf0hjbhQtO4OUh/HO+6w (comment: Promoter analysis) ------- COMMENT: 7777e3bab77e1283 7 icgHlILYf0hjbhQtO4OUh/HO+6w (comment: Promoter analysis) ------- COMMENT: 7792eb73b01ab912 1 qtUQgEMnkx/nMGlWJlTWq4/ZT9Q (comment: SDS-PAGE followed by western blotting and proteinase K treatment. Dot plots with extracts for pellet, soluble and total cell fractions with and without pre-treatment with 2% SDS. SDD-AGE gels of samples treated at room temperature and at 95°C, both with and without curing with GdnHCl.) ------- COMMENT: 7792eb73b01ab912 2 PoD/X5wog2XSob3LlHeeirMaY7s (comment: Phenotype is inherited in non-Mendelian manner, via protein aggregates (prion-like)) ------- COMMENT: 779cb119eb8301e0 34 EBDtec6LozGidbGA83U9EFSWOT0 ------- COMMENT: 779cb119eb8301e0 35 4zbnQ5N3OvTOuQQrYwwLzHGqKGE (comment: polysome profile) ------- COMMENT: 779cb119eb8301e0 36 BrfIbYRWSQXRJuEBq4H5V6IodbY fig 2B ------- COMMENT: 779cb119eb8301e0 37 iBbX2sDkL6uH6Slmt1+LO2KaCk8 figure 2C ------- COMMENT: 779cb119eb8301e0 39 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig 4 ------- COMMENT: 779cb119eb8301e0 40 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 779cb119eb8301e0 41 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig 4 ------- COMMENT: 779cb119eb8301e0 43 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 779cb119eb8301e0 57 MRTl98rKQY4u69JsQq5X5gUaN4M fig 5C ------- COMMENT: 779cb119eb8301e0 58 MRTl98rKQY4u69JsQq5X5gUaN4M fig 5C ------- COMMENT: 779cb119eb8301e0 59 MRTl98rKQY4u69JsQq5X5gUaN4M fig 5C ------- COMMENT: 779cb119eb8301e0 60 MRTl98rKQY4u69JsQq5X5gUaN4M fig 5C ------- COMMENT: 779cb119eb8301e0 61 /MeFOUj/tH9NgUX9Yb5pgdGWrFE Fig 6a ------- COMMENT: 779cb119eb8301e0 62 /MeFOUj/tH9NgUX9Yb5pgdGWrFE Fig 6a ------- COMMENT: 779cb119eb8301e0 63 /MeFOUj/tH9NgUX9Yb5pgdGWrFE Fig 6a ------- COMMENT: 779cb119eb8301e0 64 /MeFOUj/tH9NgUX9Yb5pgdGWrFE Fig 6a ------- COMMENT: 779cb119eb8301e0 65 lPer2ncYxtkCY1bCl13x5ILsRG4 Figure 6E ------- COMMENT: 779cb119eb8301e0 66 lPer2ncYxtkCY1bCl13x5ILsRG4 Figure 6E ------- COMMENT: 779cb119eb8301e0 67 T9uaeTqCYhA8EU0WkpUZ15olvkQ figure 6F ------- COMMENT: 779cb119eb8301e0 68 T9uaeTqCYhA8EU0WkpUZ15olvkQ figure 6F ------- COMMENT: 779cb119eb8301e0 69 TBMsUvnlYcDpEmeGUAH2Kz1ylZ4 Fig 7 ------- COMMENT: 779cb119eb8301e0 70 TBMsUvnlYcDpEmeGUAH2Kz1ylZ4 Fig 7 ------- COMMENT: 779cb119eb8301e0 71 TBMsUvnlYcDpEmeGUAH2Kz1ylZ4 Fig 7 ------- COMMENT: 779cb119eb8301e0 72 TBMsUvnlYcDpEmeGUAH2Kz1ylZ4 Fig 7 ------- COMMENT: 779cb119eb8301e0 73 kTvB5rbZANgdq/lfui571ZaBluI Fig 7B ------- COMMENT: 779cb119eb8301e0 74 kTvB5rbZANgdq/lfui571ZaBluI Fig 7B ------- COMMENT: 779cb119eb8301e0 75 kTvB5rbZANgdq/lfui571ZaBluI Fig 7B ------- COMMENT: 779cb119eb8301e0 76 kTvB5rbZANgdq/lfui571ZaBluI Fig 7B ------- COMMENT: 779cb119eb8301e0 77 4zbnQ5N3OvTOuQQrYwwLzHGqKGE (comment: polysome profile) ------- COMMENT: 779cb119eb8301e0 78 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 779cb119eb8301e0 79 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 779cb119eb8301e0 81 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 77b8b1e0a2875e4f 1 Apm6T5QQNCiZn+hyRr4Gd22GgV8 After 15 min, vacuoles were visible in all three strains, and this observa- tion demonstrates that, as in budding yeast [8, 9], endocy- tosis does not require a functional type V myosin. ------- COMMENT: 77b8b1e0a2875e4f 2 Apm6T5QQNCiZn+hyRr4Gd22GgV8 After 15 min, vacuoles were visible in all three strains, and this observa- tion demonstrates that, as in budding yeast [8, 9], endocy- tosis does not require a functional type V myosin. ------- COMMENT: 77b8b1e0a2875e4f 3 zdaPWM8M0IJKxASkBUl1/BKgnns vacuoles in myo52 were smaller (0.21  0.14 m). (Figure 1) ------- COMMENT: 77bdec1cc4f8a9e5 28 PY8VEKgk7qU2pwmYKnzl483I5hc (comment: at genes) ------- COMMENT: 77d6af7bf719b589 2 5/bTljEdhnePFKMcWRGfHcYQpgk (comment: CHECK abolished) ------- COMMENT: 77d6af7bf719b589 16 ILhCwwUNO1bq/TRVPRO6o9boGAc (comment: CHECK at anaphase?) ------- COMMENT: 77d6af7bf719b589 20 ILhCwwUNO1bq/TRVPRO6o9boGAc (comment: at anaphase?) ------- COMMENT: 77dd875a9dd1dce4 20 68I2PPKrLocaMgS/nSnC7SR+xoM (comment: same as rad51d alone) ------- COMMENT: 77dd875a9dd1dce4 40 2FQq8dJaGxp+Tv7Mtx3w3Kh1IIY (comment: same as taz1d alone) ------- COMMENT: 77dd875a9dd1dce4 41 2FQq8dJaGxp+Tv7Mtx3w3Kh1IIY (comment: same as taz1d alone) ------- COMMENT: 77dd875a9dd1dce4 42 2FQq8dJaGxp+Tv7Mtx3w3Kh1IIY (comment: same as taz1d alone) ------- COMMENT: 77dd875a9dd1dce4 43 2FQq8dJaGxp+Tv7Mtx3w3Kh1IIY (comment: same as taz1d alone) ------- COMMENT: 77dd875a9dd1dce4 44 2FQq8dJaGxp+Tv7Mtx3w3Kh1IIY (comment: same as taz1d alone) ------- COMMENT: 77dd875a9dd1dce4 45 2FQq8dJaGxp+Tv7Mtx3w3Kh1IIY (comment: same as taz1d alone) ------- COMMENT: 77dd875a9dd1dce4 46 2FQq8dJaGxp+Tv7Mtx3w3Kh1IIY (comment: same as taz1d alone) ------- COMMENT: 77f717dc87015fe5 2 UrSU2pgkrHwM0fvJRSPQHOF7EgA (comment: Auto-phosphorylation occurred in the presence of ATP in vitro) ------- COMMENT: 77f717dc87015fe5 4 34q+bSZkNpHFc4MsjJ11g//pkfY (comment: Auto-thiophosphorylation occurred in the presence of ATP gamma-S in vitro) ------- COMMENT: 77f717dc87015fe5 5 34q+bSZkNpHFc4MsjJ11g//pkfY (comment: Auto-thiophosphorylation occurred in the presence of ATP gamma-S in vitro) ------- COMMENT: 77f717dc87015fe5 6 BsmFa//horpW76Fs92r4y/n0LBY (comment: of condensin complex) ------- COMMENT: 77f717dc87015fe5 11 TkVXoRCPTP4TUu5f+sWXXGTGkMY (comment: CHECK full-length Cut14 present; not sure how to interpret this) ------- COMMENT: 77f717dc87015fe5 13 TkVXoRCPTP4TUu5f+sWXXGTGkMY (comment: CHECK full-length Cut14 present; not sure how to interpret this) ------- COMMENT: 77f717dc87015fe5 14 TkVXoRCPTP4TUu5f+sWXXGTGkMY (comment: CHECK full-length Cut14 present; not sure how to interpret this) ------- COMMENT: 77f717dc87015fe5 15 MdCGfBAwy6B6FWCZHo+3fARnygc (comment: only captured the OEX Experiment) ------- COMMENT: 77f717dc87015fe5 17 lsGshnFttkoDpvctpX5bNSOoCtI (comment: only captured the OEX experiment) ------- COMMENT: 7803457eba12e494 2 fSF0u34g++n+Z9bW1UWdNs+CnLg Fig 2C, 2D, 2G ------- COMMENT: 7803457eba12e494 3 fSF0u34g++n+Z9bW1UWdNs+CnLg Fig 2C, 2D, 2G ------- COMMENT: 7803457eba12e494 4 cg4jEXeioLszCggk+0GJsar7NPo Fig 4H ------- COMMENT: 7803457eba12e494 5 Fye2ZONmFva6tn1h88sFaN1pAhA Fig 4F, 4H ------- COMMENT: 7803457eba12e494 7 +T5wf8JS07CfaxxC9arLokmlxjs Fig S2A, S2C ------- COMMENT: 7803457eba12e494 8 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: 7803457eba12e494 11 jfqywyOOjGdAZDM711/CIUXr0B0 Fig 2AB ------- COMMENT: 7803457eba12e494 12 jfqywyOOjGdAZDM711/CIUXr0B0 Fig 2AB ------- COMMENT: 7803457eba12e494 13 06RGRv6eMFjM1IQPXon6kF19DP8 Fig 2 B ------- COMMENT: 7803457eba12e494 14 06RGRv6eMFjM1IQPXon6kF19DP8 Fig 2 B ------- COMMENT: 7803457eba12e494 15 jfqywyOOjGdAZDM711/CIUXr0B0 Fig 2AB ------- COMMENT: 7803457eba12e494 16 jfqywyOOjGdAZDM711/CIUXr0B0 Fig 2AB ------- COMMENT: 7803457eba12e494 17 7BdhN/tLh9Xc/SOh8ZkNtnwHQPg Fig 2C (comment: vw: this represents Yoshi's suggestion" An Ark1 reduction can become a reason of merotelic attachment, which is also caused by a defect in kinetochore structures.)" ------- COMMENT: 7803457eba12e494 18 TV84vCOfix5Yk+1TJCL5Sfp26zM Fig 2C (comment: this represents Yoshi's suggestion" An Ark1 reduction can become a reason of merotelic attachment, which is also caused by a defect in kinetochore structures.)" (comment: CHECK has_penetrance high , assayed_using ark1) ------- COMMENT: 7803457eba12e494 19 ggKjklHO2Uz3kUjoDIlDevB34oQ Fig 2F ------- COMMENT: 7803457eba12e494 20 ggKjklHO2Uz3kUjoDIlDevB34oQ Fig 2F ------- COMMENT: 7803457eba12e494 21 hobAwDDkcGv8lsWinHMoy3Abxoo Fig 2E ------- COMMENT: 7803457eba12e494 22 hobAwDDkcGv8lsWinHMoy3Abxoo Fig 2E ------- COMMENT: 7803457eba12e494 23 WkadTvRlNWenw5b+frc+nicyUho Fig 2F ------- COMMENT: 7803457eba12e494 29 ywCsB7ZTEFqtb3n+o2YWea16rvw fig S4B ------- COMMENT: 7803457eba12e494 30 CdGJkpdT8o7O/3Fn7WIGSS+xLOk Fig S4A ------- COMMENT: 7803457eba12e494 31 CdGJkpdT8o7O/3Fn7WIGSS+xLOk Fig S4A ------- COMMENT: 7803457eba12e494 32 O+ogVpvOU5jD1UOIYSW5k9q1UZE fig 4b ------- COMMENT: 7803457eba12e494 33 O+ogVpvOU5jD1UOIYSW5k9q1UZE fig 4b ------- COMMENT: 7803457eba12e494 34 /A8yRiCY+eNZFl4L/mLnXXU2oJQ Fig 4C ------- COMMENT: 7803457eba12e494 35 /A8yRiCY+eNZFl4L/mLnXXU2oJQ Fig 4C ------- COMMENT: 7803457eba12e494 36 8Bfe9Cak+Lc8NE4KYnce9AOU+B8 Fig 4E ------- COMMENT: 7803457eba12e494 37 8Bfe9Cak+Lc8NE4KYnce9AOU+B8 Fig 4E ------- COMMENT: 7803457eba12e494 38 KiRbQLP6bxiYbxcvaiykp2tWHpk Fig 4f ------- COMMENT: 7803457eba12e494 39 KiRbQLP6bxiYbxcvaiykp2tWHpk Fig 4f ------- COMMENT: 7803457eba12e494 40 KiRbQLP6bxiYbxcvaiykp2tWHpk Fig 4f ------- COMMENT: 7803457eba12e494 41 KiRbQLP6bxiYbxcvaiykp2tWHpk Fig 4f ------- COMMENT: 7803457eba12e494 42 BOxDxegsDiMSDwbZfchTbsPYSKE Fig 4G ------- COMMENT: 7803457eba12e494 43 BOxDxegsDiMSDwbZfchTbsPYSKE Fig 4G ------- COMMENT: 7803457eba12e494 44 GnSGXeHwG9ypB9gbwG1U5xE1GyA Fig S4A ------- COMMENT: 7803457eba12e494 45 GnSGXeHwG9ypB9gbwG1U5xE1GyA Fig S4A ------- COMMENT: 7803457eba12e494 46 GnSGXeHwG9ypB9gbwG1U5xE1GyA Fig S4A ------- COMMENT: 7803457eba12e494 47 doS38X1V3m45G9L4Lv70VYTaEZw (Figures S4D and S4E) ------- COMMENT: 7803457eba12e494 48 0ARXa8nV/JKkrkNvQmG+awzM/eA (Figures S4D and S4E) ------- COMMENT: 7803457eba12e494 49 GnSGXeHwG9ypB9gbwG1U5xE1GyA Fig S4A ------- COMMENT: 7803457eba12e494 50 GnSGXeHwG9ypB9gbwG1U5xE1GyA Fig S4A ------- COMMENT: 7803457eba12e494 53 rLJ6fgRno0UNkBVQ/R+oEd1nuWk (comment: CHECK specifically, biorientation) ------- COMMENT: 7803457eba12e494 54 rLJ6fgRno0UNkBVQ/R+oEd1nuWk (comment: CHECK specifically, biorientation) ------- COMMENT: 7803457eba12e494 55 jl0LUY3SuiGHi6jMta0D31H+fQo Here we identify a conserved Hrk1-interacting motif (HIM) in Pds5 and a Pds5-interacting motif (PIM) in Hrk1 in fission yeast. Mutations in either motif result in the displacement of Hrk1 from centromeres. We also show that the mechanism of Pds5-dependent Hrk1 recruitment is conserved in human cells | (Figure 1 E) Thus, although Eso1, Wpl1, and Hrk1 all bind to the same surface of Pds5, we assume that they do not always compete for binding because of the excess amounts of Pds5 in the cells. Thus, HIM in Pds5 and PIM in Hrk1 are required solely for centromeric Hrk1 localization and its function, at least in the context of targeting the CPC to centromeres. ------- COMMENT: 781f89391335e10b 8 cmn3VMwBVk6123IEV3ocJ4SWdnE inhibition of origin firing requires intra-S checkpoint (fig. 5) ------- COMMENT: 781f89391335e10b 9 cmn3VMwBVk6123IEV3ocJ4SWdnE inhibition of origin firing requires intra-S checkpoint (fig. 5) ------- COMMENT: 781f89391335e10b 10 m/aSUzVN40BQooqk+HF6chD5ywQ replication forks slow independently of intra-S checkpoint (fig. 6) ------- COMMENT: 781f89391335e10b 11 EXwEtwiKJYX8HoyPMbZkoU1sX6o (table S1) ------- COMMENT: 781f89391335e10b 12 TMvrTrpj4SnjzRML1W+uAl+EgEo replication forks stall with partial dependence on intra-S checkpoint (fig. 6) ------- COMMENT: 781f89391335e10b 13 TMvrTrpj4SnjzRML1W+uAl+EgEo replication forks stall with partial dependence on intra-S checkpoint (fig. 6) ------- COMMENT: 781f89391335e10b 16 cmn3VMwBVk6123IEV3ocJ4SWdnE inhibition of origin firing requires intra-S checkpoint (fig. 5) ------- COMMENT: 781f89391335e10b 17 TMvrTrpj4SnjzRML1W+uAl+EgEo replication forks stall with partial dependence on intra-S checkpoint (fig. 6) ------- COMMENT: 784a98102f711f7f 4 IPobgffsyHjN8hTQFAFl2kQYF8I (comment: assayed using reporter based on S. cerevisiae MFA2) ------- COMMENT: 784a98102f711f7f 12 IPobgffsyHjN8hTQFAFl2kQYF8I (comment: assayed using reporter based on S. cerevisiae MFA2) ------- COMMENT: 784a98102f711f7f 13 IPobgffsyHjN8hTQFAFl2kQYF8I (comment: assayed using reporter based on S. cerevisiae MFA2) ------- COMMENT: 784a98102f711f7f 15 IPobgffsyHjN8hTQFAFl2kQYF8I (comment: assayed using reporter based on S. cerevisiae MFA2) ------- COMMENT: 784a98102f711f7f 16 IPobgffsyHjN8hTQFAFl2kQYF8I (comment: assayed using reporter based on S. cerevisiae MFA2) ------- COMMENT: 7857fadc3a2fd43a 10 dSXNtyBWVOjnHImFCvFP7ST1xkE The decrease in cell growth on maltose medium is suppressed by neighboring wild-type cells but not by agl1 delta cells, which are defective in maltase secretion. ------- COMMENT: 7857fadc3a2fd43a 15 VBY8I/zrAyLjEKTgEUO/KhoQpsY in response to carbon source change from glucose to maltose (comment: CHECK regulates agl1) ------- COMMENT: 7857fadc3a2fd43a 16 VBY8I/zrAyLjEKTgEUO/KhoQpsY in response to carbon source change from glucose to maltose (comment: CHECK regulates agl1) ------- COMMENT: 7881e95072db6f1d 579 0V+1+P006cpxw5F0quZB3moqrhY Inferred from in vitro biochemical assay using K63-linked di-ubiquitinase ------- COMMENT: 789cb7143f17138e 16 svBxNnTw+yb5Uct0Z8oNIifoRu0 (comment: same pathway) ------- COMMENT: 78ac09df4c6c5d08 4 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: 78e9527109bc9ba8 1 JTtxkodc4xqgJGKW5sVdzPowLbw (Figure 2a). However, in ΔSPCC417.09c cells, sulfur depletion did not induce the cleavage of GFP-Atg8, indicating that autophagy was not induced. ------- COMMENT: 78e9527109bc9ba8 2 Myk/HneLUozAsMrTWhWFMcXi2Sk The induction of sdr1+ expression, yeast cell growth was significantly suppressed (Figure 2d), indicating that excessive expression of sdr1+ plays an active role in sup- pressing yeast cell growth. ------- COMMENT: 78e9527109bc9ba8 4 z7gR91jCjBoUnG8KJVzsMeonqIQ Additionally, we confirmed gene expressions that showed significant differences due to the deletion of sdr1+ through a real-time polymerase chain reaction (PCR) assay (Figure 3a). ------- COMMENT: 78e9527109bc9ba8 5 z7gR91jCjBoUnG8KJVzsMeonqIQ Additionally, we confirmed gene expressions that showed significant differences due to the deletion of sdr1+ through a real-time polymerase chain reaction (PCR) assay (Figure 3a). ------- COMMENT: 78e9527109bc9ba8 9 z+MNUED4kB0vyZ243CHboTWIs2Q However, dal51+ is an adjacent gene to sdr1+ (Figure S1). Therefore, the increased expression of dal51+ in Δsdr1 cells was potentially due to the loss of the regulatory sequence of dal51+ expression rather than the deletion of the sdr1+. ------- COMMENT: 78e9527109bc9ba8 10 9WQCOTZV98NgTyVN8DLGsoQ1CJY (Figure 3a). However, dal51+ is an adjacent gene to sdr1+ (Figure S1). Therefore, the increased expression of dal51+ in Δsdr1 cells was potentially due to the loss of the regulatory sequence of dal51+ expression rather than the deletion of the sdr1+. ------- COMMENT: 78e9527109bc9ba8 14 z3q9aYUD1BNNFVfyq87dWFG9D8M These include mei2+, which encodes the master meiosis-regulator (Harigaya et al., 2006; Sugiyama et al., 2016), and phosphate depletion-responsive genes ecl3+, pho1+, and pho84+, ------- COMMENT: 78e9527109bc9ba8 16 z3q9aYUD1BNNFVfyq87dWFG9D8M These include mei2+, which encodes the master meiosis-regulator (Harigaya et al., 2006; Sugiyama et al., 2016), and phosphate depletion-responsive genes ecl3+, pho1+, and pho84+, ------- COMMENT: 78e9527109bc9ba8 18 z3q9aYUD1BNNFVfyq87dWFG9D8M These include mei2+, which encodes the master meiosis-regulator (Harigaya et al., 2006; Sugiyama et al., 2016), and phosphate depletion-responsive genes ecl3+, pho1+, and pho84+, ------- COMMENT: 78e9527109bc9ba8 20 z7gR91jCjBoUnG8KJVzsMeonqIQ Additionally, we confirmed gene expressions that showed significant differences due to the deletion of sdr1+ through a real-time polymerase chain reaction (PCR) assay (Figure 3a). ------- COMMENT: 78e9527109bc9ba8 22 z7gR91jCjBoUnG8KJVzsMeonqIQ Additionally, we confirmed gene expressions that showed significant differences due to the deletion of sdr1+ through a real-time polymerase chain reaction (PCR) assay (Figure 3a). ------- COMMENT: 78e9527109bc9ba8 23 juVM0S9L0Z99o6d7DEXNigJDXFE In contrast, in Δsdr1 diploid cells, almost no sporulation occurred under nitrogen or sulfur starvation, and the sporulation rate during phosphate starvation was also significantly less. ------- COMMENT: 78e9527109bc9ba8 24 juVM0S9L0Z99o6d7DEXNigJDXFE In contrast, in Δsdr1 diploid cells, almost no sporulation occurred under nitrogen or sulfur starvation, and the sporulation rate during phosphate starvation was also significantly less. ------- COMMENT: 78e9527109bc9ba8 26 GMZhm1UNs64TRbQXwy+n0g9OqLE We found that, similar to a phosphate-depleted environ- ment (Ohtsuka et al., 2023), the expression levels in Δpho7 cells were significantly reduced under sulfur- depleted conditions (Figure 4). ------- COMMENT: 78e9527109bc9ba8 27 GMZhm1UNs64TRbQXwy+n0g9OqLE We found that, similar to a phosphate-depleted environ- ment (Ohtsuka et al., 2023), the expression levels in Δpho7 cells were significantly reduced under sulfur- depleted conditions (Figure 4). ------- COMMENT: 78e9527109bc9ba8 31 7n2w3cAODKLiGlNT1bpfn7S7Q1o In Δfal1 cells, sulfur depletion induced the cleavage of GFP-Atg8, resulting in free-GFP, which con- firms the occurrence of autophagy (Figure 2a). ------- COMMENT: 78e9527109bc9ba8 32 MeDROcWQ1/YXTmxWZI3nf8oJ7XM autophagy was triggered in ΔSPCC417.09c cells under nitrogen depletion but not under sulfur depletion. ------- COMMENT: 78e9527109bc9ba8 33 Z5KyGXlxmC0NuKj+n3kigqLJryg Contrary to our predic- tion, the presence or absence of sdr1+ did not have signif- icant effect on atg1+ and atg20+ induction by sulfur starvation. ------- COMMENT: 78e9527109bc9ba8 34 Z5KyGXlxmC0NuKj+n3kigqLJryg Contrary to our predic- tion, the presence or absence of sdr1+ did not have signif- icant effect on atg1+ and atg20+ induction by sulfur starvation. ------- COMMENT: 78e9527109bc9ba8 35 z7gR91jCjBoUnG8KJVzsMeonqIQ Additionally, we confirmed gene expressions that showed significant differences due to the deletion of sdr1+ through a real-time polymerase chain reaction (PCR) assay (Figure 3a). ------- COMMENT: 78e9527109bc9ba8 36 g4LwglpJOCAWgl+Uorvyd9HaQzY similar to sdr1+, overexpression of fal1+ did not rectify the morphological abnormalities of Δecl cells under sulfur depletion. ------- COMMENT: 78e9527109bc9ba8 37 PvB+a/Yn0GfQeFvW30EvlsIbKpg Although the morphological abnormalities of Δsdr1 cells under sulfur depletion were rectified by overexpres- sing sdr1+, those of Δecls cells were not (Figure 5). ------- COMMENT: 78e9527109bc9ba8 38 M4dlCZWg1dkiWpZ49yNITyS/7n4 In this regard, we also confirmed that the sdr1+ deletion mutant exhibited a shorter CLS than the wild-type during the stationary phase in our assay system (Figure 6). ------- COMMENT: 791d831636b06eee 3 DHKmqGyZ7YK1kG1yvVGsvGva4oI There were a negligible number of BODIPY 493/503-stained droplets throughout those elongated double knockout cells compared to positive controls (Figure 1F,G). ------- COMMENT: 791d831636b06eee 4 2PYP6wqk/QJrU3cCRs2WC56xlW8 This was especially true in the case of Are1p. mYFP-Are1p and mYFP-Are2p were both localized throughout the nuclear and cortical/peripheral ER (Figure 2A,B). We repeated these experiments in wild-type genetic backgrounds and saw qualitatively similar YFP signal patterns (Figure 2C,D). Thus, the localizations of these two enzymes do not provide evidence to explain polarized lipid droplet formation in either cdc25-22 or wild-type fission yeast cells ------- COMMENT: 791d831636b06eee 5 2PYP6wqk/QJrU3cCRs2WC56xlW8 This was especially true in the case of Are1p. mYFP-Are1p and mYFP-Are2p were both localized throughout the nuclear and cortical/peripheral ER (Figure 2A,B). We repeated these experiments in wild-type genetic backgrounds and saw qualitatively similar YFP signal patterns (Figure 2C,D). Thus, the localizations of these two enzymes do not provide evidence to explain polarized lipid droplet formation in either cdc25-22 or wild-type fission yeast cells ------- COMMENT: 791d831636b06eee 6 2PYP6wqk/QJrU3cCRs2WC56xlW8 This was especially true in the case of Are1p. mYFP-Are1p and mYFP-Are2p were both localized throughout the nuclear and cortical/peripheral ER (Figure 2A,B). We repeated these experiments in wild-type genetic backgrounds and saw qualitatively similar YFP signal patterns (Figure 2C,D). Thus, the localizations of these two enzymes do not provide evidence to explain polarized lipid droplet formation in either cdc25-22 or wild-type fission yeast cells ------- COMMENT: 791d831636b06eee 7 2PYP6wqk/QJrU3cCRs2WC56xlW8 This was especially true in the case of Are1p. mYFP-Are1p and mYFP-Are2p were both localized throughout the nuclear and cortical/peripheral ER (Figure 2A,B). We repeated these experiments in wild-type genetic backgrounds and saw qualitatively similar YFP signal patterns (Figure 2C,D). Thus, the localizations of these two enzymes do not provide evidence to explain polarized lipid droplet formation in either cdc25-22 or wild-type fission yeast cells ------- COMMENT: 791d831636b06eee 8 mN3ufNO88yd/mGO23J8Y4mE+qhk analysis revealed that both strains had reduced whole-cell and lipid droplet TAG levels (Figure 3F,I). ------- COMMENT: 791d831636b06eee 9 mN3ufNO88yd/mGO23J8Y4mE+qhk analysis revealed that both strains had reduced whole-cell and lipid droplet TAG levels (Figure 3F,I). ------- COMMENT: 791d831636b06eee 10 mN3ufNO88yd/mGO23J8Y4mE+qhk analysis revealed that both strains had reduced whole-cell and lipid droplet TAG levels (Figure 3F,I). ------- COMMENT: 791d831636b06eee 11 mN3ufNO88yd/mGO23J8Y4mE+qhk analysis revealed that both strains had reduced whole-cell and lipid droplet TAG levels (Figure 3F,I). ------- COMMENT: 791d831636b06eee 12 6AxHcegky6FYAFCS8qbEyhxU0oM Yeast cells lacking both genes (plh1Δdga1Δ) had no droplets but instead showed vesicle-shaped BODIPY 493/503-stained structures when grown in YE5S (Figure 3J). ------- COMMENT: 791d831636b06eee 13 mIcaxgd1iHInWJvKO2KliEGnRh0 The same cells were not viable when grown in YPO (Figure S2B,C). ------- COMMENT: 791d831636b06eee 16 90K6i6i7h2Mhg6XbrSP0XdxEXkM Thus, plh1Δdga1Δ double knockouts appear to have hampered droplet biogenesis events and it is probable that TAG plays a crucial role in the ER escape hatch mechanism with minimal amounts needed even for SE lipid droplet formation [40]. ------- COMMENT: 791d831636b06eee 17 90K6i6i7h2Mhg6XbrSP0XdxEXkM Thus, plh1Δdga1Δ double knockouts appear to have hampered droplet biogenesis events and it is probable that TAG plays a crucial role in the ER escape hatch mechanism with minimal amounts needed even for SE lipid droplet formation [40]. ------- COMMENT: 791d831636b06eee 18 gNMBWX54ulObo7Z65grexvE9JZ4 As expected, these cells contained negligible amounts of TAG after lysis and TLC analysis (Figure 3K). ------- COMMENT: 791d831636b06eee 19 pXiETOxUjl0ZhAc5OFJspGOyBmQ mYFP-Dga1p and mYFP-Plh1p were both localized throughout the nuclear and cortical/peripheral ER (Figure 4A,B). ------- COMMENT: 791d831636b06eee 20 pXiETOxUjl0ZhAc5OFJspGOyBmQ mYFP-Dga1p and mYFP-Plh1p were both localized throughout the nuclear and cortical/peripheral ER (Figure 4A,B). ------- COMMENT: 791d831636b06eee 21 pXiETOxUjl0ZhAc5OFJspGOyBmQ mYFP-Dga1p and mYFP-Plh1p were both localized throughout the nuclear and cortical/peripheral ER (Figure 4A,B). ------- COMMENT: 791d831636b06eee 22 pXiETOxUjl0ZhAc5OFJspGOyBmQ mYFP-Dga1p and mYFP-Plh1p were both localized throughout the nuclear and cortical/peripheral ER (Figure 4A,B). ------- COMMENT: 791d831636b06eee 23 pXiETOxUjl0ZhAc5OFJspGOyBmQ mYFP-Dga1p and mYFP-Plh1p were both localized throughout the nuclear and cortical/peripheral ER (Figure 4A,B). ------- COMMENT: 7927a4b16214fe51 1 4CYTlF2LH4PocIwYTilECi7TVfw (comment: hhf2 and hhf3 are wild-type. Only hhf2 is mutated) ------- COMMENT: 7927a4b16214fe51 2 dSUBbhdyN6NyTCY90MN2yrdOEwo (comment: hhf1 and hhf3 are wild-type. Only hhf2 is mutated) ------- COMMENT: 7927a4b16214fe51 3 aJjQfpx870H2IpSBeUOAdDfboPo (comment: CHECK camptothecin sensitivity) ------- COMMENT: 7927a4b16214fe51 4 dSUBbhdyN6NyTCY90MN2yrdOEwo (comment: hhf1 and hhf3 are wild-type. Only hhf2 is mutated) ------- COMMENT: 7927a4b16214fe51 5 aJjQfpx870H2IpSBeUOAdDfboPo (comment: CHECK camptothecin sensitivity) ------- COMMENT: 7927a4b16214fe51 6 aJjQfpx870H2IpSBeUOAdDfboPo (comment: CHECK camptothecin sensitivity) ------- COMMENT: 7927a4b16214fe51 7 N2K9nuTolE7LWEUl5ky2pmAnGhI (comment: CHECK trichostatin A sensitivity) ------- COMMENT: 7927a4b16214fe51 8 dSUBbhdyN6NyTCY90MN2yrdOEwo (comment: hhf1 and hhf3 are wild-type. Only hhf2 is mutated) ------- COMMENT: 7927a4b16214fe51 9 dSUBbhdyN6NyTCY90MN2yrdOEwo (comment: hhf1 and hhf3 are wild-type. Only hhf2 is mutated) ------- COMMENT: 7927a4b16214fe51 10 Dv4PqFgwIC6vq6PN064lGGliggc (comment: When both hhf1 and hhf3 are deleted, hhf2-K5/12R cells are sensitive to camptothecin) ------- COMMENT: 7927a4b16214fe51 11 Dv4PqFgwIC6vq6PN064lGGliggc (comment: When both hhf1 and hhf3 are deleted, hhf2-K5/12R cells are sensitive to camptothecin) ------- COMMENT: 7927a4b16214fe51 12 7JseYZzz9UxoSMH2pB/S+EGlue0 (comment: When both hht1 and hht3 are deleted, hht2-K9R and hht2-K56 cells are sensitive to camptothecin) ------- COMMENT: 7927a4b16214fe51 13 45sxvH94hWA+9iow3EBLcgRMxNc (comment: When both hht1 and hht3 are deleted, hht3-K9R and hht2-K56 cells are sensitive to camptothecin) ------- COMMENT: 7927a4b16214fe51 14 JY5T/B0hgZu8V0jKH5DSfi6KPBU (comment: grows normally at 25 degrees but not at 30 degrees) ------- COMMENT: 7927a4b16214fe51 16 aJjQfpx870H2IpSBeUOAdDfboPo (comment: CHECK camptothecin sensitivity) ------- COMMENT: 7927a4b16214fe51 21 aJjQfpx870H2IpSBeUOAdDfboPo (comment: CHECK camptothecin sensitivity ------- COMMENT: 7927a4b16214fe51 22 gM7uY6KHhH9pn8D22qDZasr7rug (comment: CHECK methyl methanesulfonate sensitivity) ------- COMMENT: 7927a4b16214fe51 28 JY5T/B0hgZu8V0jKH5DSfi6KPBU grows normally at 25 degrees but not at 30 degrees ------- COMMENT: 7927a4b16214fe51 29 JY5T/B0hgZu8V0jKH5DSfi6KPBU (comment: grows normally at 25 degrees but not at 30 degrees) ------- COMMENT: 7927a4b16214fe51 31 vLtet6gkJkkwl35VdFxIKf3HxYA (comment: same as swi1delta alone) ------- COMMENT: 7927a4b16214fe51 32 JY5T/B0hgZu8V0jKH5DSfi6KPBU (comment: grows normally at 25 degrees but not at 30 degrees) ------- COMMENT: 7927a4b16214fe51 40 4CYTlF2LH4PocIwYTilECi7TVfw (comment: hhf2 and hhf3 are wild-type. Only hhf2 is mutated) ------- COMMENT: 7927a4b16214fe51 56 AvTWx+5n9aaceAKbOEa6D1jAubs (comment: 25 degrees; same as mst1-L344S alone) ------- COMMENT: 7927a4b16214fe51 57 r6oP6q4sY843EVbbAhjCph035u4 (comment: CHECK same as nmt81-vid21 alone) ------- COMMENT: 796a4851ed930759 20 eHA5RMWCRmTHLm2t3JgzesgddpQ ( is not a resolvase - makes symmetric cuts on opposed strands across the junction but does not convert products to linear DNA molecules) ------- COMMENT: 796a4851ed930759 21 166jrMEEZeV+ErqI1sQkWlrGh88 (comment: specific for dsDNA at ds/ssDNA junction) ------- COMMENT: 797e7567a449409b 1 7JVIqcEBAWNQpr8zvaKlw11O5vc (comment: bound by the C-terminal dsrbd domain) ------- COMMENT: 7999293403fb7249 1 gZNo/xWKvKsHWWGOazkQEqseH2U (Fig. 1) ------- COMMENT: 7999293403fb7249 2 gZNo/xWKvKsHWWGOazkQEqseH2U (Fig. 1) ------- COMMENT: 7999293403fb7249 3 PEijj9MIrNSBSecC4MlXqO1v4Zk (Fig. 3) ------- COMMENT: 7999293403fb7249 4 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 7999293403fb7249 5 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 7999293403fb7249 6 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 7999293403fb7249 7 GkI93EYSqP6NybAtsJjxO2fy3Ic (Fig. 4D) ------- COMMENT: 7999293403fb7249 8 GkI93EYSqP6NybAtsJjxO2fy3Ic (Fig. 4D) ------- COMMENT: 7999293403fb7249 9 GkI93EYSqP6NybAtsJjxO2fy3Ic (Fig. 4D) ------- COMMENT: 7999293403fb7249 10 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: 7999293403fb7249 11 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: 7999293403fb7249 12 VUMagwwUEp+HC5aJcs9wTo5GBF4 (Fig. 5D and E) ------- COMMENT: 7999293403fb7249 13 iMlVJjSclU5vaYJ0uPqYIMBEQx0 Here we identified an additional mechanism of MOR inhibition by the SIN through Sid2 phosphorylation of Sog2. ------- COMMENT: 7999293403fb7249 14 kuRdbpnFrU9sjksgW9GQwEQ1424 (Fig. 5) ------- COMMENT: 79a78f7ee5ca3dc5 1 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 79a78f7ee5ca3dc5 2 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 79a78f7ee5ca3dc5 3 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 79a78f7ee5ca3dc5 4 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 79a78f7ee5ca3dc5 5 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 79a78f7ee5ca3dc5 6 kHdOAkxyIc88c07XjJf0Tzb8l3s Fig. 1B, S3 ------- COMMENT: 79a78f7ee5ca3dc5 7 kHdOAkxyIc88c07XjJf0Tzb8l3s Fig. 1B, S3 ------- COMMENT: 79a78f7ee5ca3dc5 8 kHdOAkxyIc88c07XjJf0Tzb8l3s Fig. 1B, S3 ------- COMMENT: 79a78f7ee5ca3dc5 9 kHdOAkxyIc88c07XjJf0Tzb8l3s Fig. 1B, S3 ------- COMMENT: 79a78f7ee5ca3dc5 10 nLUQxqO7XIgHOT9D6uceA0P76QA Fig. 2A (comment: assayed with plo1 GFP) ------- COMMENT: 79a78f7ee5ca3dc5 11 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 79a78f7ee5ca3dc5 12 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 79a78f7ee5ca3dc5 13 oC3zAXkKuEPXJoxMX5W5VuRpFs4 Fig. 3B/C ------- COMMENT: 79a78f7ee5ca3dc5 14 mGT9O9MMx7M90uvZMscjACbfNRM Fig. 3B/C. ------- COMMENT: 79a78f7ee5ca3dc5 15 GZ/vJ2xCWLYxqDW5ImP7MMNuOGE Fig. 3B/C ------- COMMENT: 79a78f7ee5ca3dc5 16 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: 79a78f7ee5ca3dc5 17 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: 79a78f7ee5ca3dc5 18 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: 79a78f7ee5ca3dc5 19 THw2I19k1sJPzl0NkRZ91C7BBwI Fig. 3E, S9 ------- COMMENT: 79a78f7ee5ca3dc5 20 THw2I19k1sJPzl0NkRZ91C7BBwI Fig. 3E, S9 ------- COMMENT: 79a78f7ee5ca3dc5 21 ifrqlcy7G0f4EFipSEbm7b0gMNU Fig. S2A ------- COMMENT: 79a78f7ee5ca3dc5 22 ifrqlcy7G0f4EFipSEbm7b0gMNU Fig. S2A ------- COMMENT: 79a78f7ee5ca3dc5 23 ifrqlcy7G0f4EFipSEbm7b0gMNU Fig. S2A ------- COMMENT: 79a78f7ee5ca3dc5 24 +Ek/y8FI1jr63JOLTj8F9c2kaEQ Fig. S2B ------- COMMENT: 79a78f7ee5ca3dc5 25 +Ek/y8FI1jr63JOLTj8F9c2kaEQ Fig. S2B ------- COMMENT: 79a78f7ee5ca3dc5 26 +Ek/y8FI1jr63JOLTj8F9c2kaEQ Fig. S2B ------- COMMENT: 79a78f7ee5ca3dc5 27 NTzqOyFLy4TesPVE8RIfn1dft08 Fig. S6A/B ------- COMMENT: 79a78f7ee5ca3dc5 28 lnnKMeUh19HKevDybRnr5G/EhqU Fig. S6C/D ------- COMMENT: 79a78f7ee5ca3dc5 29 lnnKMeUh19HKevDybRnr5G/EhqU Fig. S6C/D ------- COMMENT: 79a78f7ee5ca3dc5 30 82rmuE2qaElnGkj7kcgJKzGNVCs Fig. S7 ------- COMMENT: 79a78f7ee5ca3dc5 31 N37xxxrAlgzqUDtu0sMaL2u0hvI Fig. S10 ------- COMMENT: 79a78f7ee5ca3dc5 35 zbQj+JhjuUnwEM6kFpUCCr3hDXc (Fig 3C), showed a similar fraction of mono-oriented chromosomes as wild-type cells ------- COMMENT: 79a78f7ee5ca3dc5 36 wfAaERzFlcRu9hF1olp2+IS2nZk rarely showed a delay in bi-orienting chromosomes that had been pulled towards one SPB (Fig 3B,D; supplementary Fig S4F online). ------- COMMENT: 79c4b71f272e27d6 50 GWgs9r9yLOQqn+7WFMPmzNTlqVM fig2e ------- COMMENT: 79cfb1652794d9bc 2 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 79cfb1652794d9bc 3 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 79cfb1652794d9bc 4 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 79cfb1652794d9bc 5 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 79cfb1652794d9bc 6 Dd1ADzgiGLHzFen/1qUv9Bv68Sk Figure 4 (comment: ALSO TFIIH but not sure which sunbunit) ------- COMMENT: 79cfb1652794d9bc 7 Dd1ADzgiGLHzFen/1qUv9Bv68Sk Figure 4 (comment: ALSO TFIIH but not sure which sunbunit) ------- COMMENT: 79cfb1652794d9bc 8 iqeAlJyX1iMDETB85p+10L3p6gs Figure 4 (comment: CHECK SAP155K700E restored splicing to prp10-1) ------- COMMENT: 79cfb1652794d9bc 9 7wnyPcu2r8UFN3UWfgUqmFxIp0o Figure 3 & S4 (comment: ALSO TFIIH but not sure which sunbunit) ------- COMMENT: 79cfb1652794d9bc 10 7wnyPcu2r8UFN3UWfgUqmFxIp0o Figure 3 & S4 (comment: ALSO TFIIH but not sure which sunbunit) ------- COMMENT: 79cfb1652794d9bc 11 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 79cfb1652794d9bc 12 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 79ecbc11c0699149 2 dquSggBTOiJBBACjuKfCMatq2pY (comment: genes in extensions are assayed as represntative of highly transcribed genes) ------- COMMENT: 7a38c014384c9f45 5 3fA2G7LV7vjLpWTGdqM8VZ1wisU (comment: assayed using purified HeLa histone octamers) ------- COMMENT: 7a38c014384c9f45 6 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment: same as either single mutant) ------- COMMENT: 7a38c014384c9f45 7 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment: same as either single mutant) ------- COMMENT: 7a38c014384c9f45 8 1bEOJ/uo4tUIV5/cK8I36avE7WI (comemnt: same as either single mutant) ------- COMMENT: 7a38c014384c9f45 9 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment: same as either single mutant) ------- COMMENT: 7a38c014384c9f45 10 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment: same as either single mutant) ------- COMMENT: 7a38c014384c9f45 11 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment: same as either single mutant) ------- COMMENT: 7a38c014384c9f45 12 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment: same as either single mutant) ------- COMMENT: 7a38c014384c9f45 13 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment: same as either single mutant) ------- COMMENT: 7a38c014384c9f45 14 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment: same as either single mutant) ------- COMMENT: 7a38c014384c9f45 15 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment: same as either single mutant) ------- COMMENT: 7a6602af54fdea54 5 aSH1UoHehaOSoogIzECd67T4pn4 figure 1 ------- COMMENT: 7a6602af54fdea54 6 aSH1UoHehaOSoogIzECd67T4pn4 figure 1 ------- COMMENT: 7a6602af54fdea54 7 aSH1UoHehaOSoogIzECd67T4pn4 figure 1 ------- COMMENT: 7a6602af54fdea54 8 aSH1UoHehaOSoogIzECd67T4pn4 figure 1 ------- COMMENT: 7ac74c1d6b6f1521 6 DK9FwGx8GprsZ0cZGvjm4qKEfds (comment: intron 2) ------- COMMENT: 7ac7ea383ba244b2 1 OFPguoxyiZ3vBqdIj9ZWZsIO2tw These data show that Fin1p- mediated compaction of the chromosomes is not func- tionally related to mitotic chromosome condensation and the mechanism by which it occurs remains obscure. ------- COMMENT: 7ac7ea383ba244b2 2 OFPguoxyiZ3vBqdIj9ZWZsIO2tw These data show that Fin1p- mediated compaction of the chromosomes is not func- tionally related to mitotic chromosome condensation and the mechanism by which it occurs remains obscure. ------- COMMENT: 7ac7ea383ba244b2 3 OFPguoxyiZ3vBqdIj9ZWZsIO2tw These data show that Fin1p- mediated compaction of the chromosomes is not func- tionally related to mitotic chromosome condensation and the mechanism by which it occurs remains obscure. ------- COMMENT: 7ac7ea383ba244b2 4 OFPguoxyiZ3vBqdIj9ZWZsIO2tw These data show that Fin1p- mediated compaction of the chromosomes is not func- tionally related to mitotic chromosome condensation and the mechanism by which it occurs remains obscure. ------- COMMENT: 7ac7ea383ba244b2 8 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 7ac7ea383ba244b2 9 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 7ac7ea383ba244b2 10 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 7ac7ea383ba244b2 11 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 7ac7ea383ba244b2 12 Zo9kwzKX8C8TvhcBNXm6pyDqzSU fig 6 resulted in a synthetic arrest at metaphase of mitosis. This contrasts with the G1 cell cycle arrest of pim1-d1 single mutant cells (Krien et al., 1998). ------- COMMENT: 7ac7ea383ba244b2 13 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: 7ac7ea383ba244b2 14 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 7ac7ea383ba244b2 15 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 7ac7ea383ba244b2 16 XbRsGRehbS+i4dEpwxqjnhEcrDg Figure 6 which was enhanced by the presence of the pim1-d1 mutation at 36°C to include all mitotic cells (arrowed ------- COMMENT: 7ac7ea383ba244b2 17 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: 7ac7ea383ba244b2 18 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: 7ac7ea383ba244b2 19 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: 7ac7ea383ba244b2 20 B9On+0uC91d6vp1XquamzAHRHzY The double ®n1Dbub1D mutants were viable, though substantially retarded in colony formation and showed extensive chromosome segregation defects (Figure 7A). ------- COMMENT: 7ac7ea383ba244b2 21 B9On+0uC91d6vp1XquamzAHRHzY The double ®n1Dbub1D mutants were viable, though substantially retarded in colony formation and showed extensive chromosome segregation defects (Figure 7A). ------- COMMENT: 7ac7ea383ba244b2 23 dwmpUDO+Wde/NMb0ZMWg21iedOU Double mutants between ®n1D and the temperature-sensitive allele rad21-K1 (Tatebayashi et al., 1998) were synthetically lethal at all temperatures ------- COMMENT: 7ac7ea383ba244b2 24 JMQD6VQr6pUNM4L58QB/OT8mWO0 (in. non mitotic cells) ------- COMMENT: 7ac7ea383ba244b2 26 0yyeyIGmyhsDRX9JXKZmyyahlrA suggesting the lethal synthetic interaction between ®n1D and cut11 might relate of the nuclear envelope rather than SPB anchoring during mitosis ------- COMMENT: 7ad9eb5a73f1bab1 3 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 4 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 5 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 6 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 7 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 8 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 9 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 10 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 11 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 12 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 13 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 14 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 15 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 16 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 17 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 18 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 19 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 20 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 21 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 22 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 23 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 24 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 25 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 26 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 27 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 28 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 29 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7ad9eb5a73f1bab1 30 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 7ad9eb5a73f1bab1 31 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 7ad9eb5a73f1bab1 32 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 7ad9eb5a73f1bab1 33 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 7ad9eb5a73f1bab1 34 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 7ad9eb5a73f1bab1 35 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 7ad9eb5a73f1bab1 36 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 7ad9eb5a73f1bab1 37 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 7ad9eb5a73f1bab1 38 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 7ad9eb5a73f1bab1 39 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 7ad9eb5a73f1bab1 40 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 7ad9eb5a73f1bab1 41 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 7ad9eb5a73f1bab1 42 2GQ0b5iy5W0nY9FFuZUnroSD1CM Fig. S2 and text ------- COMMENT: 7ad9eb5a73f1bab1 43 2GQ0b5iy5W0nY9FFuZUnroSD1CM Fig. S2 and text ------- COMMENT: 7ad9eb5a73f1bab1 44 2GQ0b5iy5W0nY9FFuZUnroSD1CM Fig. S2 and text ------- COMMENT: 7ad9eb5a73f1bab1 45 2GQ0b5iy5W0nY9FFuZUnroSD1CM Fig. S2 and text ------- COMMENT: 7ad9eb5a73f1bab1 46 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 7ad9eb5a73f1bab1 47 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 7ad9eb5a73f1bab1 48 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 7ad9eb5a73f1bab1 49 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 7ad9eb5a73f1bab1 50 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 7ad9eb5a73f1bab1 51 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 7ad9eb5a73f1bab1 52 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 7ad9eb5a73f1bab1 53 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 7ad9eb5a73f1bab1 54 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 7ad9eb5a73f1bab1 55 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 7ad9eb5a73f1bab1 56 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 7ad9eb5a73f1bab1 57 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 7ad9eb5a73f1bab1 58 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 7ad9eb5a73f1bab1 59 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 7ad9eb5a73f1bab1 60 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 7ad9eb5a73f1bab1 61 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 7ad9eb5a73f1bab1 62 aNKxUDmLkPWzGXk1THjjLBZPcI4 Fig. S12 ------- COMMENT: 7ad9eb5a73f1bab1 63 aNKxUDmLkPWzGXk1THjjLBZPcI4 Fig. S12 ------- COMMENT: 7ad9eb5a73f1bab1 64 aNKxUDmLkPWzGXk1THjjLBZPcI4 Fig. S12 ------- COMMENT: 7ad9eb5a73f1bab1 65 aNKxUDmLkPWzGXk1THjjLBZPcI4 Fig. S12 ------- COMMENT: 7ad9eb5a73f1bab1 66 aNKxUDmLkPWzGXk1THjjLBZPcI4 Fig. S12 ------- COMMENT: 7ad9eb5a73f1bab1 67 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 7ad9eb5a73f1bab1 68 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 7ad9eb5a73f1bab1 69 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 7ad9eb5a73f1bab1 70 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 7ad9eb5a73f1bab1 71 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 7ad9eb5a73f1bab1 72 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 7ad9eb5a73f1bab1 73 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 7ad9eb5a73f1bab1 74 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 7ad9eb5a73f1bab1 75 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 7ad9eb5a73f1bab1 76 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 7ad9eb5a73f1bab1 77 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 7ad9eb5a73f1bab1 78 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 7ad9eb5a73f1bab1 79 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 7ad9eb5a73f1bab1 80 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: 7b30a1cc7d87ace2 57 noyX1hwehi/64BZ+bjJW7SNWlG8 (comment: phosphorylates rgf1 during HU response, to maintain of protein lcoation in nucleus) ------- COMMENT: 7bbe318b26434db4 1 Nedh1r7TjfebCOSy8N/8iqB4Ims Fig1 supp data ------- COMMENT: 7bbe318b26434db4 2 Nedh1r7TjfebCOSy8N/8iqB4Ims Fig1 supp data ------- COMMENT: 7bbe318b26434db4 3 Nedh1r7TjfebCOSy8N/8iqB4Ims Fig1 supp data ------- COMMENT: 7bbe318b26434db4 4 Nedh1r7TjfebCOSy8N/8iqB4Ims Fig1 supp data ------- COMMENT: 7bbe318b26434db4 5 Nedh1r7TjfebCOSy8N/8iqB4Ims Fig1 supp data ------- COMMENT: 7bbe318b26434db4 6 Nedh1r7TjfebCOSy8N/8iqB4Ims Fig1 supp data ------- COMMENT: 7bbe318b26434db4 7 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 7bbe318b26434db4 8 EsdDg2Wd/l5Tsy4qA+b1oqFR7b0 Fig 1a,b,c ------- COMMENT: 7bbe318b26434db4 9 P/QKXS9CMs3cWfeWB07YHCHklOs Fig 1a shows the lem2 chromatin binding domain is not required to restrict enhancement of the NC ratio of rae1-167 ------- COMMENT: 7bbe318b26434db4 11 5Ajb9/sEGSlWq1kQxezXIiC5cso Fig2a,b,c ------- COMMENT: 7bbe318b26434db4 12 zgkguKifbw3QlKqp3+1GvhtcPlI Fig2 d,e,f,g,h supp fig6b ------- COMMENT: 7bbe318b26434db4 13 wQ0Kx153L1EJKkmXGo69yfn4Qeg Fig3a,b ------- COMMENT: 7bbe318b26434db4 14 K4nxH71hb5LFinkeplopuZ/AYb4 Fig 3c ------- COMMENT: 7bbe318b26434db4 15 aVaRAOW+xFfre0PWDyjjk+wWXPo Fig3c,d, (comment: CHECK OE SUPRESSOR OF NEM1delta lem2 supresses the increased NC ratio of nem1 delta) ------- COMMENT: 7bbe318b26434db4 16 kk6iIns0wO0WwX+IfbJnW6n6hHk Fig3f ------- COMMENT: 7bbe318b26434db4 17 YI9yGLkIwSZW1l8Ro0Uw3DoFTqQ supp Fig6a ------- COMMENT: 7bbe318b26434db4 18 4DxGbIRj+Wjdd6OfjytbBQgF2jY Supp Fig6 ------- COMMENT: 7bbe318b26434db4 19 6F6mvPiIMzbm0j6hMDhPsD6TXEw Fig4 a,c ------- COMMENT: 7bbe318b26434db4 20 5K5jsjWxrRPbIsFfyz9SXJ5P/oo Fig 4,b,c (comment: CHECK ENHANCER OF N/C ratio of lem2/rae1) ------- COMMENT: 7bbe318b26434db4 21 mB1krpri6tmvGZImVrDjsGOqvvo Fig 4a,c ------- COMMENT: 7bbe318b26434db4 22 kk6iIns0wO0WwX+IfbJnW6n6hHk Fig3f ------- COMMENT: 7bbe318b26434db4 23 K4nxH71hb5LFinkeplopuZ/AYb4 Fig 3c ------- COMMENT: 7bbe318b26434db4 24 K4nxH71hb5LFinkeplopuZ/AYb4 Fig 3c ------- COMMENT: 7bbe318b26434db4 25 +lOC+ZdEKnq1TknIfxZCJE59SCg supp Fig 7 (comment: supression of lem2delta) ------- COMMENT: 7bbe318b26434db4 26 StfkHr1OXVMG4AHZ3jt1G13UK+0 supp data Fig 1b,c ------- COMMENT: 7bbe318b26434db4 27 +kO+g2T6p89lfPWegexWEzCsIDM Fig1 and Fig1supp data ------- COMMENT: 7bbe318b26434db4 28 h86cfyVGg7xtVhKGXA3Q6lxutOs Fig3d ------- COMMENT: 7bbe318b26434db4 29 xplXL1m6EpxlS2BwBmzW+eA//NQ Fig3d, OE lem2 (comment: supresses the increased NC ratio of rae1-167) ------- COMMENT: 7bbe318b26434db4 30 7dN9jUXAFPP89Ls1r3YoliWajNg Fig3e (comment: suppression of nem1delta) ------- COMMENT: 7bbe318b26434db4 31 M9hf5+zj5g8ZyOpygTNTyptK4kc Fig 3e ------- COMMENT: 7bbe318b26434db4 33 PFLMdMyHutCLuC2m5Ly5aK1JHiQ Fig 1 (comment: normal compaction) ------- COMMENT: 7bbe318b26434db4 34 mB1krpri6tmvGZImVrDjsGOqvvo Fig 4a,c ------- COMMENT: 7bbe318b26434db4 35 6F6mvPiIMzbm0j6hMDhPsD6TXEw Fig4 a,c ------- COMMENT: 7bf1fc1e6f06a613 1 Ski7jVa1khHMpk9Nnbq/Bf1W+eM figure 1C, 1E ------- COMMENT: 7bf1fc1e6f06a613 3 Ski7jVa1khHMpk9Nnbq/Bf1W+eM figure 1C, 1E ------- COMMENT: 7bf1fc1e6f06a613 4 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: 7bf1fc1e6f06a613 5 rGoHYzRj3Jvr9rA7qfuhNAUcJbA figure 2 (comment: requires phosphorylated T89, T154, T155 to bind Nbs1 FHA domain) ------- COMMENT: 7bf1fc1e6f06a613 6 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: 7bf1fc1e6f06a613 7 8F8k7RN2g6ngxsyw43muHmOOOzA figure 3B ------- COMMENT: 7bf1fc1e6f06a613 8 8F8k7RN2g6ngxsyw43muHmOOOzA figure 3B ------- COMMENT: 7bf1fc1e6f06a613 9 8F8k7RN2g6ngxsyw43muHmOOOzA figure 3B ------- COMMENT: 7bf1fc1e6f06a613 10 8F8k7RN2g6ngxsyw43muHmOOOzA figure 3B ------- COMMENT: 7bf1fc1e6f06a613 11 8F8k7RN2g6ngxsyw43muHmOOOzA figure 3B ------- COMMENT: 7bf1fc1e6f06a613 12 oJN940NlrCwYqfXpg4AMW5yPjdw figure 3C ------- COMMENT: 7bf1fc1e6f06a613 13 oJN940NlrCwYqfXpg4AMW5yPjdw figure 3C ------- COMMENT: 7bf1fc1e6f06a613 14 oJN940NlrCwYqfXpg4AMW5yPjdw figure 3C ------- COMMENT: 7bf1fc1e6f06a613 15 oJN940NlrCwYqfXpg4AMW5yPjdw figure 3C ------- COMMENT: 7bf1fc1e6f06a613 16 oJN940NlrCwYqfXpg4AMW5yPjdw figure 3C ------- COMMENT: 7bf1fc1e6f06a613 17 WR4eY9sTthO3PnzjKXFbZFO6hx0 figure 3D ------- COMMENT: 7bf1fc1e6f06a613 18 WR4eY9sTthO3PnzjKXFbZFO6hx0 figure 3D ------- COMMENT: 7bf1fc1e6f06a613 19 WR4eY9sTthO3PnzjKXFbZFO6hx0 figure 3D ------- COMMENT: 7bf1fc1e6f06a613 20 WR4eY9sTthO3PnzjKXFbZFO6hx0 figure 3D ------- COMMENT: 7bf1fc1e6f06a613 21 WR4eY9sTthO3PnzjKXFbZFO6hx0 figure 3D ------- COMMENT: 7bf1fc1e6f06a613 22 WR4eY9sTthO3PnzjKXFbZFO6hx0 figure 3D ------- COMMENT: 7bf1fc1e6f06a613 23 fBg5Xd5d1NNZ14nMgFWzbAraZKE We therefore introduced a synthetic CT15 peptide into an endonuclease assay containing the MR complex, but lacking Nbs1 (Fig. 4A). Strikingly, the CT15 peptide stimulated the endonuclease activity of MR similarly to the unphosphorylated, full-length Ctp1 (Fig. 4B). Moreover, stimulation of MR at higher concentrations of the CT15 peptide (100 μM) was comparable to the maximal levels achieved with the MRN complex and phosphorylated full-length Ctp1 (Ctp1p in Fig. 4C). ------- COMMENT: 7bf1fc1e6f06a613 24 aSH1UoHehaOSoogIzECd67T4pn4 figure 1 ------- COMMENT: 7bf1fc1e6f06a613 25 aSH1UoHehaOSoogIzECd67T4pn4 figure 1 ------- COMMENT: 7bf1fc1e6f06a613 26 aSH1UoHehaOSoogIzECd67T4pn4 figure 1 ------- COMMENT: 7bf1fc1e6f06a613 28 iyMraj5qmLEJtewo/fSEFxmllqQ figure 2D ------- COMMENT: 7bf1fc1e6f06a613 29 iyMraj5qmLEJtewo/fSEFxmllqQ figure 2D ------- COMMENT: 7bf1fc1e6f06a613 30 FHkRXhIRTMUTituCJXGWi089Lbw figure 2E ------- COMMENT: 7bf1fc1e6f06a613 31 FHkRXhIRTMUTituCJXGWi089Lbw figure 2E ------- COMMENT: 7bf893a86d94196c 2 42IG26ur7vQoaySvq1fRBIVInws (comment: CONDITION restrictive temp 32) ------- COMMENT: 7bf893a86d94196c 3 tRjAecHtDbzAH/5K+4DdEh8g0fQ (comment: CONDITION restrictive temp 36) ------- COMMENT: 7bf893a86d94196c 5 DFzn9Z3/vk9i3U1h7lv1Ghfixa4 (comment: CONDITION 29 degrees C) ------- COMMENT: 7bf893a86d94196c 7 AYCjehAu9sPE8NAVGuF7gexrZ5A (comment: CONDITION 27 degrees C) ------- COMMENT: 7bf893a86d94196c 8 DFzn9Z3/vk9i3U1h7lv1Ghfixa4 (comment: CONDITION 29 degrees C) ------- COMMENT: 7bf893a86d94196c 9 AYCjehAu9sPE8NAVGuF7gexrZ5A (comment: CONDITION 27 degrees C) ------- COMMENT: 7bf893a86d94196c 10 SQ8YEqCwSBzVSa/iiwM0Jy15EZg (condition 25 degrees C) ------- COMMENT: 7bf893a86d94196c 11 SQ8YEqCwSBzVSa/iiwM0Jy15EZg (comment: CONDITION 25 degrees C) ------- COMMENT: 7bf893a86d94196c 12 SQ8YEqCwSBzVSa/iiwM0Jy15EZg (comment: CONDITION 25 degrees C) ------- COMMENT: 7c2513328f82d364 1 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 7c2513328f82d364 2 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 7c2513328f82d364 3 9m+5L5kEwhOnF8foCoBh+5mxVN4 Figure 5e ------- COMMENT: 7c2513328f82d364 4 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 7c2513328f82d364 5 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 7c2513328f82d364 7 2G1ThBaB169UATfmKUzqhtGVh/w Figure 4. (comment: Sty1 interacted and phosphorylated Rpb1-CTD at Ser5) ------- COMMENT: 7c2513328f82d364 8 2G1ThBaB169UATfmKUzqhtGVh/w Figure 4. (comment: Sty1 interacted and phosphorylated Rpb1-CTD at Ser5) ------- COMMENT: 7c2513328f82d364 9 D90Pfvz5MDXKgCr8NnCMgGZ7ttQ (comment: Sty1 interacted with Rpb1-CTD) ------- COMMENT: 7c2513328f82d364 10 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 7c2513328f82d364 11 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 7c2513328f82d364 12 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 7c2513328f82d364 13 j8lx0cjXOogtvtAy6trm2XWxJ9Q fig2a ------- COMMENT: 7c2513328f82d364 18 IJhozGvAtybFbpC4pLgt+84cgCo fig? (comment: under calf alkaline phosphatase treated) ------- COMMENT: 7c2513328f82d364 23 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 7c2513328f82d364 24 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 7c2513328f82d364 25 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 7c2513328f82d364 26 JJ61Rr+BzZ+yYkS4ndaMB26Ouxc 2B ------- COMMENT: 7c2513328f82d364 27 JJ61Rr+BzZ+yYkS4ndaMB26Ouxc 2B ------- COMMENT: 7c2513328f82d364 28 JJ61Rr+BzZ+yYkS4ndaMB26Ouxc 2B ------- COMMENT: 7c2513328f82d364 30 6ZgX/1GFhS3j8zAGghRo9NuUJOc As shown in Figure 3C and D, upon H2O2 stress, Rpb1 was recruited to the promoter and extensively phosphorylated at Ser5. ------- COMMENT: 7c2513328f82d364 31 6ZgX/1GFhS3j8zAGghRo9NuUJOc As shown in Figure 3C and D, upon H2O2 stress, Rpb1 was recruited to the promoter and extensively phosphorylated at Ser5. ------- COMMENT: 7c2513328f82d364 33 8+ZIsTar4IYngzRalbB9uVFJgbw In the absence of Pin1, Ser5 phosphorylated Rpb1 was associated and accumulated at the promoter region following H2O2 stress but was defective in entering elongation to generate transcripts of the corresponding genes (Figure 1C) ------- COMMENT: 7c2513328f82d364 34 SPwHySqqOJsxih0be/f74FPhLRc In line with these results, Ser2 phosphorylation of Rpb1-CTD, which facilitated transcription elongation, was reduced in pin1 mutant as a secondary effect derived from defect in transcription initiation to elongation (Figure 3A and B). ------- COMMENT: 7c2513328f82d364 36 yGbMN54koYw0G6ihqn21e419cKQ In line with these results, Ser2 phosphorylation of Rpb1-CTD, which facilitated transcription elongation, was reduced in pin1 mutant as a secondary effect derived from defect in transcription initiation to elongation (Figure 3A and B). ------- COMMENT: 7c2513328f82d364 40 Kx4t+AElJJcogLNh1t2J1fuejFc Intriguingly, upon oxidative stress, the association between Rpb1 and Sty1 was decreased in wild type cells and up regulated in the pin1 mutant with reduced phosphorylation of Ser2 (Figure 4B). ------- COMMENT: 7c2513328f82d364 43 XUxeeVgyiZEtqoodwT4lJ+UUpJY These results suggested that, in addition to the binding to theRpb1-CTD, the isomerization activity was also required for the fu ------- COMMENT: 7c2513328f82d364 44 UrIMPNFCW9vcuzGj1eo3zITz0pc Figure 6c (comment: The anti-myc, anti-Rpb1 CTD (8WG16) and anti-pS5-Rpb1 CTD (H14) antibodies were used for immunoprecipitation.) ------- COMMENT: 7c2513328f82d364 45 2G1ThBaB169UATfmKUzqhtGVh/w Figure 4. (comment: Sty1 interacted and phosphorylated Rpb1-CTD at Ser5) ------- COMMENT: 7c2513328f82d364 46 RaxQAPfna+6A9ZKCxKST+/X02+8 Ssu72, but not with the GST control in the pull down experiment. These results suggested that Pin1 directly interacted with and recruited Ssu72 for pSer5 dephosphorylation to facilitate progression of transcription important for cellular response to oxidative stress ------- COMMENT: 7c2513328f82d364 47 tstc2KE4KhRgalH149Jp+FoWjVc (comment: CHECK is this +H2o2) the intracellular level of ROS was elevated in pin1 and ssu72 mutants (Figure 6H), ------- COMMENT: 7c2513328f82d364 48 R8gEyac+Z86ZCMR+BOiXMxs5jh8 (comment: CHECK is this +h2o2) the intracellular level of ROS was elevated in pin1 and ssu72 mutants (Figure 6H), ------- COMMENT: 7c2513328f82d364 50 hNCD04UpWOa//cC5A/9duo6/sCA (Figure 5D) ------- COMMENT: 7c2513328f82d364 51 LcxzzPvVnlnZiDwsZuce34W57bI Figure 2c (comment: CHECK this replaces the sty1 WT annotation Should this be normal? i.e. normal for the conditions? ) ------- COMMENT: 7c2513328f82d364 52 LcxzzPvVnlnZiDwsZuce34W57bI Figure 2c (comment: CHECK this replaces the sty1 WT annotation Should this be normal? i.e. normal for the conditions? ) ------- COMMENT: 7c2f0cff132ea0bb 18 zKQ3OYTeCAFiShSZOw+vKsNwF+U (comment: CHECK spacing is wrong as well as occupancy) ------- COMMENT: 7c5c740e8a4e0e0c 1 EaJ1cIJCgxH1h7dmT6mEA3YTf4A Figure 1d ------- COMMENT: 7c5c740e8a4e0e0c 2 Ld17rIK+EPvvSll8ZZCtyZm8/zg (comment: CHECK bent spindle in meiosis I of skp1-a7 is suppressed by rec8∆) ------- COMMENT: 7c5c740e8a4e0e0c 3 m//+EaK+5v88/2XdsWze0C3QYio (comment: CHECK bent spindle in meiosis I of skp1-a7 is suppressed by rec8∆) ------- COMMENT: 7c5c740e8a4e0e0c 4 WGlk2EZ2HHtyyh5K1OtY5I9FCKs (comment: CHECK during meiosis I) ------- COMMENT: 7c5c740e8a4e0e0c 5 bVsL7x4+sBpc8Uxo2ipM9nHZWSo (comment: CHECK bent spindle in meiosis I of skp1-a7 is suppressed by rec8∆) ------- COMMENT: 7c5c740e8a4e0e0c 6 75w7eSnzhv+rzyuEYMNv/BHFPgc (comment: CHECK bent spindle in meiosis I of skp1-a7 is suppressed by rec8∆) ------- COMMENT: 7c5c740e8a4e0e0c 7 mywygMzqC6QIJwxFGFv7zTYWQjc figure 1a ------- COMMENT: 7c5c740e8a4e0e0c 8 EaJ1cIJCgxH1h7dmT6mEA3YTf4A figure 1d ------- COMMENT: 7c5c740e8a4e0e0c 9 EaJ1cIJCgxH1h7dmT6mEA3YTf4A figure 1d ------- COMMENT: 7c5c740e8a4e0e0c 10 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 7c5c740e8a4e0e0c 11 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 7c5c740e8a4e0e0c 12 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 7c5c740e8a4e0e0c 13 etstGK1JRFYsvr4eVHgIXA/aR7I fig3d ------- COMMENT: 7c5c740e8a4e0e0c 14 p8SgknyuBDrKpf5LkPKSpMYQ37k fig3e ------- COMMENT: 7c5c740e8a4e0e0c 15 p8SgknyuBDrKpf5LkPKSpMYQ37k fig3e ------- COMMENT: 7c5c740e8a4e0e0c 16 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 7c5c740e8a4e0e0c 18 rDR41po8gfpi5g9cNpYWWk5easQ Figure 5 ------- COMMENT: 7c5c740e8a4e0e0c 19 rDR41po8gfpi5g9cNpYWWk5easQ Figure 5 ------- COMMENT: 7c5c740e8a4e0e0c 20 rDR41po8gfpi5g9cNpYWWk5easQ Figure 5 ------- COMMENT: 7c5c740e8a4e0e0c 22 rDR41po8gfpi5g9cNpYWWk5easQ Figure 5 ------- COMMENT: 7c5c740e8a4e0e0c 25 /ChKLnjfSaPsDfyP4sFkeAOTl40 Figure 5c ------- COMMENT: 7c5c740e8a4e0e0c 26 /ChKLnjfSaPsDfyP4sFkeAOTl40 Figure 5c ------- COMMENT: 7c5c740e8a4e0e0c 27 /ChKLnjfSaPsDfyP4sFkeAOTl40 Figure 5c ------- COMMENT: 7c5c740e8a4e0e0c 28 /ChKLnjfSaPsDfyP4sFkeAOTl40 Figure 5c ------- COMMENT: 7c5c740e8a4e0e0c 29 1Sm0+hKUZ/gEPwKD9fyd/ro8PvA Figure 5d ------- COMMENT: 7c5c740e8a4e0e0c 30 1Sm0+hKUZ/gEPwKD9fyd/ro8PvA Figure 5d ------- COMMENT: 7c5c740e8a4e0e0c 31 1Sm0+hKUZ/gEPwKD9fyd/ro8PvA Figure 5d ------- COMMENT: 7c5c740e8a4e0e0c 36 rDR41po8gfpi5g9cNpYWWk5easQ Figure 5 ------- COMMENT: 7c5c740e8a4e0e0c 37 rDR41po8gfpi5g9cNpYWWk5easQ Figure 5 ------- COMMENT: 7c5c740e8a4e0e0c 42 WGlk2EZ2HHtyyh5K1OtY5I9FCKs (comment: CHECK during meiosis I) ------- COMMENT: 7c5c740e8a4e0e0c 43 WGlk2EZ2HHtyyh5K1OtY5I9FCKs (comment: CHECK during meiosis I) ------- COMMENT: 7c5c740e8a4e0e0c 44 rU1PLFa55jvFaT+XlIrJecFqAoU (comment: CHECK strong contender for GO's "acts upstream of or within" (RO:0002264) gp-term relation) ------- COMMENT: 7c5c740e8a4e0e0c 45 rU1PLFa55jvFaT+XlIrJecFqAoU (comment: CHECK strong contender for GO's "acts upstream of or within" (RO:0002264) gp-term relation) ------- COMMENT: 7c6f6dac1e623770 2 KntnJkiXmLt+bu4ukaXFz0c82Wk figure 5B ------- COMMENT: 7c6f6dac1e623770 4 CFYJqZFU1rEhQXJEB5RmzbKieTQ figure 5B ------- COMMENT: 7c6f6dac1e623770 9 UyAEOKYPANvRqqpESXNNhDBQ3AI (fig 1) ------- COMMENT: 7c6f6dac1e623770 10 UyAEOKYPANvRqqpESXNNhDBQ3AI (fig 1) ------- COMMENT: 7c6f6dac1e623770 11 UyAEOKYPANvRqqpESXNNhDBQ3AI (fig 1) ------- COMMENT: 7c6f6dac1e623770 12 DTUGXgk2qZUYX4suMyuyDBjQ+1M (comment: CHECK *******during copper excess******) fig2 ------- COMMENT: 7c6f6dac1e623770 13 DjTqYh8vFXNxd38ppHoNR5erX2w (comment: CHECK *******during copper excess******) fig2 ------- COMMENT: 7c6f6dac1e623770 14 ihVaAQkI5j2m6h/4jb556tTn3ao Observed at this location during spore maturation by indirect immunofluorescence ------- COMMENT: 7c6f6dac1e623770 16 zPOQNuIAo36/f1ygoHfO9nlS9uU fig2 ------- COMMENT: 7c7509b7ad78573d 1 0FbS4PjxotAy1IaktOHNZ4up6E0 (Figure S2) ------- COMMENT: 7c7509b7ad78573d 2 0FbS4PjxotAy1IaktOHNZ4up6E0 (Figure S2) ------- COMMENT: 7c80e0a2a39ec200 3 U1iqHDzucs74WA5reOw3zlhvf8Y Data not shown cdc13 protein level in asynchronous culture of wee1-50 cells at restrictive temperature is reduced by 40% compared to asynchronous culture of WT cells ------- COMMENT: 7c80e0a2a39ec200 4 QolKbjAeAeoMB/DYRKoh/PnAm5o Uses elutriation synchrony. Fig5 cdc13 protein level in synchronous culture of wee1-50 cells at restrictive temperature is absent during longer G1 phase ------- COMMENT: 7c80e0a2a39ec200 5 VQxJ7Fgy4AXFizD/LrGEeQwC0lc Fig 9 ------- COMMENT: 7c80e0a2a39ec200 6 qmRe2Zk3DvHbpl7dKpKdqWgTn7Q Fig10 ------- COMMENT: 7c93f34f9f6c11b6 1 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 7c93f34f9f6c11b6 2 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 7c93f34f9f6c11b6 3 02l+3cqTh7UTFJSYjyKhzJabH68 fig1c ------- COMMENT: 7c93f34f9f6c11b6 4 02l+3cqTh7UTFJSYjyKhzJabH68 fig1c ------- COMMENT: 7c93f34f9f6c11b6 5 02l+3cqTh7UTFJSYjyKhzJabH68 fig1c ------- COMMENT: 7c93f34f9f6c11b6 6 02l+3cqTh7UTFJSYjyKhzJabH68 fig1c ------- COMMENT: 7c93f34f9f6c11b6 7 Xk4XVif8/nUlu5nQ7w+waCmWtLo As shown in Figure 2C, H2O2 induced robust phosphorylation of Spc1 in csx1D cells..... Csx1 is not necessary for Spc1 activation. ------- COMMENT: 7c93f34f9f6c11b6 8 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 7c93f34f9f6c11b6 9 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 7c93f34f9f6c11b6 10 dLr5g5+3ZehSeJ+gY4dFLBD7LlU The csx1D single mutant was less sensitive to H2O2 than the spc1D mutant, whereas the csx1D spc1D double mutant was more sensitive than the spc1D strain. These ®ndings were consistent with the idea that Csx1 and Spc1 have independent functions in oxidative stress tolerance. ------- COMMENT: 7c93f34f9f6c11b6 16 7zc8EueiEqyYNl3bLA1Xjdibee4 the large increase in atf1+ mRNA that is induced by H2O2 in wildtype cells was abolished in csx1D cells. ------- COMMENT: 7c93f34f9f6c11b6 17 wvQtt1Zj3MuSqe4eRX79MtmQ3DA This decrease correlated with a large drop in the amount of Atf1 protein (Figure 4C). ------- COMMENT: 7c93f34f9f6c11b6 18 Ovcplqg7WdCLaWTUSvsCnaQdcKk The H2O2-induced increase in expression of pcr1+ mRNA, which encodes a binding partner of Atf1, was similarly eliminated in csx1D cells (Figure 4A ------- COMMENT: 7c93f34f9f6c11b6 19 63JdiXXzHK5jnjBxSdCqQZfav5E mRNA expression levels of two other transcription factor genes involved in oxidative stress, pap1+ and prr1+, were unaffected by the csx1D mutation (Figure 4A). ------- COMMENT: 7c93f34f9f6c11b6 20 63JdiXXzHK5jnjBxSdCqQZfav5E mRNA expression levels of two other transcription factor genes involved in oxidative stress, pap1+ and prr1+, were unaffected by the csx1D mutation (Figure 4A). ------- COMMENT: 7c93f34f9f6c11b6 21 63JdiXXzHK5jnjBxSdCqQZfav5E mRNA expression levels of two other transcription factor genes involved in oxidative stress, pap1+ and prr1+, were unaffected by the csx1D mutation (Figure 4A). ------- COMMENT: 7c93f34f9f6c11b6 22 bcvJ2mOQDH7SwyqbZIOhf0qcYKs Figure 4E ------- COMMENT: 7c93f34f9f6c11b6 23 e2ynp3fAJmipbp1jzZfn6nYRuBk Figure 6A, Csx1±GFP was detected in the cytoplasm and appeared to be excluded from the nucleus. This pattern of Csx1±GFP localization was unaffected by oxidative stress. ------- COMMENT: 7c93f34f9f6c11b6 24 e2ynp3fAJmipbp1jzZfn6nYRuBk Figure 6A, Csx1±GFP was detected in the cytoplasm and appeared to be excluded from the nucleus. This pattern of Csx1±GFP localization was unaffected by oxidative stress. ------- COMMENT: 7c93f34f9f6c11b6 25 nzQ+53LeMrj7JbOBrd2EU91C4gw (Figure 6B). decreased stability in response to oxidative stress ------- COMMENT: 7c93f34f9f6c11b6 26 8rx+45pLWr7zql5YXTF33bQnt9o decreased stability in response to oxidative stress (Figure 6B) ------- COMMENT: 7c93f34f9f6c11b6 27 4qEio0jxTSbE7md1CqIUZYFTd3I (Figure 6B) decreased stability in response to oxidative stress ------- COMMENT: 7c93f34f9f6c11b6 28 4qEio0jxTSbE7md1CqIUZYFTd3I (Figure 6B) decreased stability in response to oxidative stress ------- COMMENT: 7c93f34f9f6c11b6 29 W8U2HOh5c9FHrXYcx0iyvAr6hdQ Figure 7A ------- COMMENT: 7c93f34f9f6c11b6 32 uhSuyJkMv6GGLo1Dbk6vms2LWX0 (comment: from genetics and Sty1 consensus. Later papers say Activated Sty1 also phosphorylates Csx1) ------- COMMENT: 7ca7b767d537166d 1 G0Jmna16KdoqAi13wpZ+OeCMeo4 (comment: binds O6-alkylguanine, 2-aminopurine and 2,6-diaminopurine) ------- COMMENT: 7cc0d044cd0fd080 2 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 7cc0d044cd0fd080 3 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 7cc0d044cd0fd080 4 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 7cc0d044cd0fd080 6 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 7cc0d044cd0fd080 7 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 7cc0d044cd0fd080 8 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 7cc0d044cd0fd080 10 glJW1aOyj4jhjS/C9sk9B+zU/dU Figure 4a ------- COMMENT: 7cc0d044cd0fd080 13 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 7cc0d044cd0fd080 14 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 7cc0d044cd0fd080 15 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 7cc0d044cd0fd080 17 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: 7cc0d044cd0fd080 18 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: 7cc0d044cd0fd080 19 NvYyQa7tc1oQpCaS4vE4cpgoQPM (comment: CHECK extension, of cdc25) Figure 7b ------- COMMENT: 7cc0d044cd0fd080 20 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: 7cc0d044cd0fd080 21 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: 7cc0d044cd0fd080 22 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 7cc0d044cd0fd080 23 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 7cc0d044cd0fd080 24 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 7cc0d044cd0fd080 25 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 7cc0d044cd0fd080 27 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: 7cd37f3825c76d6e 2 kW49rsDhGivgAAVyhNOfLM9t2iU (comment: endogenous ade6) ------- COMMENT: 7cd37f3825c76d6e 37 SNQ3blWY22NRpR9UopbBIpL+HXc (comment: this is the endogenous dg repeat) ------- COMMENT: 7cd37f3825c76d6e 39 vIp0zRvopKwpnM33RQ472Gauvjo (comment: abolished at exogenous RNA polII transcribed gene) ------- COMMENT: 7cd37f3825c76d6e 67 jyztoHI3LmjE0q9WFW5yYpGWQNs (comment: not sure which clrc subunit it binds to?) ------- COMMENT: 7cec44eee583f82d 13 aSX3r/a9NyoO5UaKHWOCN9pkoy8 (comment: CONDITION Grown in EMM + 200uM ZnSo) (comment: Measurements made via ICP-MS) ------- COMMENT: 7cec44eee583f82d 15 aSX3r/a9NyoO5UaKHWOCN9pkoy8 (comment: CONDITION Grown in EMM + 200uM ZnSo). (comment: Measurements made via ICP-MS) ------- COMMENT: 7cec44eee583f82d 18 aSX3r/a9NyoO5UaKHWOCN9pkoy8 (comment: CONDITION Grown in EMM + 200uM ZnSo). (comment: Measurements made via ICP-MS) ------- COMMENT: 7cec44eee583f82d 19 aAFtlSCbFyNeSVjbnEBHqMi9Zdk (comment: CONDITION Visualized via Florescence using an integrated LOZ1::GFP construct) (comment: CONDITION grown in EMM +/- ZnSo4) ------- COMMENT: 7cec44eee583f82d 20 qvxEZQNP9LCgBEoAKgxxDN1ET18 (comment: CHECK directly regulates adh4) ------- COMMENT: 7cec44eee583f82d 23 ZJG+NWcHaIhspWwPsOOpusLqSgA (comment: Via EMSA binds directly to adh4 promoter). ------- COMMENT: 7ced7ee21306a509 14 djoDCN638I4UuRgK8inSMaeTZN8 (comment: cdc12 froms a cortical spot) ------- COMMENT: 7d087bec74eeb3b6 2 C1m4M1d4/BCjvsTAp4A0Qd7IDL8 (comment: CHECK link to GEO dataset- GSE71820) ------- COMMENT: 7d087bec74eeb3b6 3 C1m4M1d4/BCjvsTAp4A0Qd7IDL8 (comment: CHECK link to GEO dataset- GSE71820) ------- COMMENT: 7d2c05ef7277f909 13 E49Ss1UWnp9pN2FhB+GyCXcdWzM (comment: centromeric) ------- COMMENT: 7d2c05ef7277f909 14 yZS8nv1DkzoZ0oCkfOzCQ5CL4h4 (comment: H3-pS10 used to detect ark1 activity) ------- COMMENT: 7d2c05ef7277f909 25 r+0DIDOhS50JzvHN/ITEolcA5TI (comment: decreased along arms) ------- COMMENT: 7d2c05ef7277f909 39 bSgnpY/XAeGXD7ZQyiihbEkZshM (comment: all tested chromosome loci) (Fig. 2g). ------- COMMENT: 7d2c05ef7277f909 40 bSgnpY/XAeGXD7ZQyiihbEkZshM (comment: all tested chromosome loci) (Fig. 2g). ------- COMMENT: 7d2c05ef7277f909 53 XfJ/SSxsv8jDd5JUHMQa5HxU+5Q (comment: CHECK condensin, which subunit assayed?) ------- COMMENT: 7d2c05ef7277f909 58 KoH3MXRlbFf0fLlNRr04jbTp3jM we engineered a Cnd2–Cnp3C fusion protein, which targets kinetochores through the Cnp3C domain32, even in pcs1Δ cells. The expression of this fusion protein largely suppresses the growth defect, sensitivity to thiabendazole (TBZ, a microtubule destabilizing drug) and the incidence of lagging chromosomes in pcs1Δ cells, whereas neither Cnd2 nor Cnp3C protein alone suppresses these phenotypes (Fig. 1c and Supplementary Fig. 7). These ------- COMMENT: 7d2c05ef7277f909 59 6fOWlrvgaZ6KX7jWnAwW6xUjyYw In contrast, the growth defect and TBZ sensitivity of pcs1Δ cells are not suppressed by the sfc3-1 mutation, whereas they are suppressed by an increase in kinetochore condensin (Fig. 1d). ------- COMMENT: 7d409d497eb075ca 1 5pkWHnIxh3PKbppSMN/ZmpbTnig we surveyed several additional ts lethal splicing mutants for silencing defects at the permissive temperature (11-14). Only particular splicing mutants affected silencing. Silencing of a centromeric cen1:ade6+ marker gene (Fig. 1A) remained intact in the presence of prp1 (Prp6Sc/Hs), prp2 (U2AFHs), prp3 (Prp3Sc/PRPF3Hs), or prp4 (PRPF4BHs) mutations ------- COMMENT: 7d409d497eb075ca 2 5pkWHnIxh3PKbppSMN/ZmpbTnig we surveyed several additional ts lethal splicing mutants for silencing defects at the permissive temperature (11-14). Only particular splicing mutants affected silencing. Silencing of a centromeric cen1:ade6+ marker gene (Fig. 1A) remained intact in the presence of prp1 (Prp6Sc/Hs), prp2 (U2AFHs), prp3 (Prp3Sc/PRPF3Hs), or prp4 (PRPF4BHs) mutations ------- COMMENT: 7d409d497eb075ca 3 5pkWHnIxh3PKbppSMN/ZmpbTnig we surveyed several additional ts lethal splicing mutants for silencing defects at the permissive temperature (11-14). Only particular splicing mutants affected silencing. Silencing of a centromeric cen1:ade6+ marker gene (Fig. 1A) remained intact in the presence of prp1 (Prp6Sc/Hs), prp2 (U2AFHs), prp3 (Prp3Sc/PRPF3Hs), or prp4 (PRPF4BHs) mutations ------- COMMENT: 7d409d497eb075ca 4 5pkWHnIxh3PKbppSMN/ZmpbTnig we surveyed several additional ts lethal splicing mutants for silencing defects at the permissive temperature (11-14). Only particular splicing mutants affected silencing. Silencing of a centromeric cen1:ade6+ marker gene (Fig. 1A) remained intact in the presence of prp1 (Prp6Sc/Hs), prp2 (U2AFHs), prp3 (Prp3Sc/PRPF3Hs), or prp4 (PRPF4BHs) mutations ------- COMMENT: 7d409d497eb075ca 5 XuGHRmj48yqUTsl36PFh2CMLEKg In contrast, mutations in prp5 (Prp46Sc/PLRG1Hs), prp8 (Prp2Sc/DHX16Hs), prp10 (Hsh155Sc/ SF3B1Hs), and prp12 (Rse1Sc/SF3B2Hs), like cwf10 and prp39, alleviated cen1:ade6+ silencing (Fig. 1B) and increased cen1:ade6+ transcript accumulation (Fig. 1C, ade6) ------- COMMENT: 7d409d497eb075ca 6 XuGHRmj48yqUTsl36PFh2CMLEKg In contrast, mutations in prp5 (Prp46Sc/PLRG1Hs), prp8 (Prp2Sc/DHX16Hs), prp10 (Hsh155Sc/ SF3B1Hs), and prp12 (Rse1Sc/SF3B2Hs), like cwf10 and prp39, alleviated cen1:ade6+ silencing (Fig. 1B) and increased cen1:ade6+ transcript accumulation (Fig. 1C, ade6) ------- COMMENT: 7d409d497eb075ca 7 XuGHRmj48yqUTsl36PFh2CMLEKg In contrast, mutations in prp5 (Prp46Sc/PLRG1Hs), prp8 (Prp2Sc/DHX16Hs), prp10 (Hsh155Sc/ SF3B1Hs), and prp12 (Rse1Sc/SF3B2Hs), like cwf10 and prp39, alleviated cen1:ade6+ silencing (Fig. 1B) and increased cen1:ade6+ transcript accumulation (Fig. 1C, ade6) ------- COMMENT: 7d409d497eb075ca 8 XuGHRmj48yqUTsl36PFh2CMLEKg In contrast, mutations in prp5 (Prp46Sc/PLRG1Hs), prp8 (Prp2Sc/DHX16Hs), prp10 (Hsh155Sc/ SF3B1Hs), and prp12 (Rse1Sc/SF3B2Hs), like cwf10 and prp39, alleviated cen1:ade6+ silencing (Fig. 1B) and increased cen1:ade6+ transcript accumulation (Fig. 1C, ade6) ------- COMMENT: 7d409d497eb075ca 9 XuGHRmj48yqUTsl36PFh2CMLEKg In contrast, mutations in prp5 (Prp46Sc/PLRG1Hs), prp8 (Prp2Sc/DHX16Hs), prp10 (Hsh155Sc/ SF3B1Hs), and prp12 (Rse1Sc/SF3B2Hs), like cwf10 and prp39, alleviated cen1:ade6+ silencing (Fig. 1B) and increased cen1:ade6+ transcript accumulation (Fig. 1C, ade6) ------- COMMENT: 7d409d497eb075ca 10 XuGHRmj48yqUTsl36PFh2CMLEKg In contrast, mutations in prp5 (Prp46Sc/PLRG1Hs), prp8 (Prp2Sc/DHX16Hs), prp10 (Hsh155Sc/ SF3B1Hs), and prp12 (Rse1Sc/SF3B2Hs), like cwf10 and prp39, alleviated cen1:ade6+ silencing (Fig. 1B) and increased cen1:ade6+ transcript accumulation (Fig. 1C, ade6) ------- COMMENT: 7d409d497eb075ca 11 XuGHRmj48yqUTsl36PFh2CMLEKg In contrast, mutations in prp5 (Prp46Sc/PLRG1Hs), prp8 (Prp2Sc/DHX16Hs), prp10 (Hsh155Sc/ SF3B1Hs), and prp12 (Rse1Sc/SF3B2Hs), like cwf10 and prp39, alleviated cen1:ade6+ silencing (Fig. 1B) and increased cen1:ade6+ transcript accumulation (Fig. 1C, ade6) ------- COMMENT: 7d409d497eb075ca 12 XuGHRmj48yqUTsl36PFh2CMLEKg In contrast, mutations in prp5 (Prp46Sc/PLRG1Hs), prp8 (Prp2Sc/DHX16Hs), prp10 (Hsh155Sc/ SF3B1Hs), and prp12 (Rse1Sc/SF3B2Hs), like cwf10 and prp39, alleviated cen1:ade6+ silencing (Fig. 1B) and increased cen1:ade6+ transcript accumulation (Fig. 1C, ade6) ------- COMMENT: 7d409d497eb075ca 13 XuGHRmj48yqUTsl36PFh2CMLEKg In contrast, mutations in prp5 (Prp46Sc/PLRG1Hs), prp8 (Prp2Sc/DHX16Hs), prp10 (Hsh155Sc/ SF3B1Hs), and prp12 (Rse1Sc/SF3B2Hs), like cwf10 and prp39, alleviated cen1:ade6+ silencing (Fig. 1B) and increased cen1:ade6+ transcript accumulation (Fig. 1C, ade6) ------- COMMENT: 7d409d497eb075ca 14 XuGHRmj48yqUTsl36PFh2CMLEKg In contrast, mutations in prp5 (Prp46Sc/PLRG1Hs), prp8 (Prp2Sc/DHX16Hs), prp10 (Hsh155Sc/ SF3B1Hs), and prp12 (Rse1Sc/SF3B2Hs), like cwf10 and prp39, alleviated cen1:ade6+ silencing (Fig. 1B) and increased cen1:ade6+ transcript accumulation (Fig. 1C, ade6) ------- COMMENT: 7d409d497eb075ca 15 XuGHRmj48yqUTsl36PFh2CMLEKg In contrast, mutations in prp5 (Prp46Sc/PLRG1Hs), prp8 (Prp2Sc/DHX16Hs), prp10 (Hsh155Sc/ SF3B1Hs), and prp12 (Rse1Sc/SF3B2Hs), like cwf10 and prp39, alleviated cen1:ade6+ silencing (Fig. 1B) and increased cen1:ade6+ transcript accumulation (Fig. 1C, ade6) ------- COMMENT: 7d409d497eb075ca 16 XuGHRmj48yqUTsl36PFh2CMLEKg In contrast, mutations in prp5 (Prp46Sc/PLRG1Hs), prp8 (Prp2Sc/DHX16Hs), prp10 (Hsh155Sc/ SF3B1Hs), and prp12 (Rse1Sc/SF3B2Hs), like cwf10 and prp39, alleviated cen1:ade6+ silencing (Fig. 1B) and increased cen1:ade6+ transcript accumulation (Fig. 1C, ade6) ------- COMMENT: 7d409d497eb075ca 17 r8qHahdhgYYfoTHxOMiHBQZ0bqM Moreover, mutants that alleviated cen1:ade6+ silencing also displayed increased levels of noncoding centromeric otr transcripts and concomitant reductions in centromeric siRNA accumulation, with prp10-1 showing the most severe silencing defects (Fig. 1C ------- COMMENT: 7d409d497eb075ca 18 r8qHahdhgYYfoTHxOMiHBQZ0bqM Moreover, mutants that alleviated cen1:ade6+ silencing also displayed increased levels of noncoding centromeric otr transcripts and concomitant reductions in centromeric siRNA accumulation, with prp10-1 showing the most severe silencing defects (Fig. 1C ------- COMMENT: 7d409d497eb075ca 19 r8qHahdhgYYfoTHxOMiHBQZ0bqM Moreover, mutants that alleviated cen1:ade6+ silencing also displayed increased levels of noncoding centromeric otr transcripts and concomitant reductions in centromeric siRNA accumulation, with prp10-1 showing the most severe silencing defects (Fig. 1C ------- COMMENT: 7d409d497eb075ca 20 r8qHahdhgYYfoTHxOMiHBQZ0bqM Moreover, mutants that alleviated cen1:ade6+ silencing also displayed increased levels of noncoding centromeric otr transcripts and concomitant reductions in centromeric siRNA accumulation, with prp10-1 showing the most severe silencing defects (Fig. 1C ------- COMMENT: 7d409d497eb075ca 21 lZTYvAzRn072/qmC6A8FTCy+HrE However, at the permissive temperature of 25°C (at which centromeric silencing was alleviated), splicing efficiency was similar to that in wild-type cells (Fig. 2A). ------- COMMENT: 7d409d497eb075ca 22 lZTYvAzRn072/qmC6A8FTCy+HrE However, at the permissive temperature of 25°C (at which centromeric silencing was alleviated), splicing efficiency was similar to that in wild-type cells (Fig. 2A). ------- COMMENT: 7d409d497eb075ca 23 lZTYvAzRn072/qmC6A8FTCy+HrE However, at the permissive temperature of 25°C (at which centromeric silencing was alleviated), splicing efficiency was similar to that in wild-type cells (Fig. 2A). ------- COMMENT: 7d409d497eb075ca 24 lZTYvAzRn072/qmC6A8FTCy+HrE However, at the permissive temperature of 25°C (at which centromeric silencing was alleviated), splicing efficiency was similar to that in wild-type cells (Fig. 2A). ------- COMMENT: 7d409d497eb075ca 25 lZTYvAzRn072/qmC6A8FTCy+HrE However, at the permissive temperature of 25°C (at which centromeric silencing was alleviated), splicing efficiency was similar to that in wild-type cells (Fig. 2A). ------- COMMENT: 7d409d497eb075ca 26 lZTYvAzRn072/qmC6A8FTCy+HrE However, at the permissive temperature of 25°C (at which centromeric silencing was alleviated), splicing efficiency was similar to that in wild-type cells (Fig. 2A). ------- COMMENT: 7d409d497eb075ca 27 IOmVN48nqX77KRQVQvaIyd6uD1E We therefore constructed strains in which the endogenous ago1+ and hrr1+ genes were replaced by cDNAs. Even in these strains, the prp10-1 mutation alleviated silencing as in wild-type cells (Fig. 2C, white colonies). ------- COMMENT: 7d409d497eb075ca 28 IOmVN48nqX77KRQVQvaIyd6uD1E We therefore constructed strains in which the endogenous ago1+ and hrr1+ genes were replaced by cDNAs. Even in these strains, the prp10-1 mutation alleviated silencing as in wild-type cells (Fig. 2C, white colonies). ------- COMMENT: 7d409d497eb075ca 29 BQt4Sgj8TvxRMCs2BMwQda9uAeY Chromatin immunoprecipitation (ChIP) revealed that splicing mutants cwf10-1 and prp10-1 (but not prp2-1) exhibited only a modest decrease in levels of H3K9me2 associated with both centromere repeats and cen1:ura4+ (Fig. 3A and fig. S3). ------- COMMENT: 7d409d497eb075ca 30 BQt4Sgj8TvxRMCs2BMwQda9uAeY Chromatin immunoprecipitation (ChIP) revealed that splicing mutants cwf10-1 and prp10-1 (but not prp2-1) exhibited only a modest decrease in levels of H3K9me2 associated with both centromere repeats and cen1:ura4+ (Fig. 3A and fig. S3). ------- COMMENT: 7d409d497eb075ca 31 Enr8e8NM4SRuEd7kH5J6kZs1zk0 (Fig. 3A and fig. S3). ------- COMMENT: 7d409d497eb075ca 32 BQt4Sgj8TvxRMCs2BMwQda9uAeY Chromatin immunoprecipitation (ChIP) revealed that splicing mutants cwf10-1 and prp10-1 (but not prp2-1) exhibited only a modest decrease in levels of H3K9me2 associated with both centromere repeats and cen1:ura4+ (Fig. 3A and fig. S3). ------- COMMENT: 7d409d497eb075ca 33 BQt4Sgj8TvxRMCs2BMwQda9uAeY Chromatin immunoprecipitation (ChIP) revealed that splicing mutants cwf10-1 and prp10-1 (but not prp2-1) exhibited only a modest decrease in levels of H3K9me2 associated with both centromere repeats and cen1:ura4+ (Fig. 3A and fig. S3). ------- COMMENT: 7d409d497eb075ca 34 BQt4Sgj8TvxRMCs2BMwQda9uAeY Chromatin immunoprecipitation (ChIP) revealed that splicing mutants cwf10-1 and prp10-1 (but not prp2-1) exhibited only a modest decrease in levels of H3K9me2 associated with both centromere repeats and cen1:ura4+ (Fig. 3A and fig. S3). ------- COMMENT: 7d409d497eb075ca 35 OjOOhxlj4agxMPK6Fg/gyEuXWNM Many splicing factors were found to specifically associate with Cid12-FLAG. These included Cwf10, Prp10, Prp5, and Prp12, which were required for centromeric silencing, along with splicing factors such as Prp3 that did not affect silencing (Fig. 4C) ------- COMMENT: 7d409d497eb075ca 39 7bmP0LoJfIN+QOFu/dmE8DCqk7Y The association of Cwf10 with Cid12 was also verified by coimmunoprecipitation (Fig. 4D). ------- COMMENT: 7d41b40fbc72d8a6 1 5r/SowRZHb4XDpzvu3mD+08aVDg (comment: only amino acid auxotrophic cell) ------- COMMENT: 7d41b40fbc72d8a6 4 5r/SowRZHb4XDpzvu3mD+08aVDg (comment: only amino acid auxotrophic cell) ------- COMMENT: 7d41b40fbc72d8a6 5 isaE1gOZtNZ9id82K1nqta0cKfc The control strain ED668 expressed ecl1+ when Mg2+ was depleted but not in a strain lacking fil1+ (Figure 2a) ------- COMMENT: 7d41b40fbc72d8a6 6 YmTxJTDzHzOjfrxGZmwhHI/Yh4o (comment: to capture target of ecl1) ------- COMMENT: 7d41b40fbc72d8a6 7 5r/SowRZHb4XDpzvu3mD+08aVDg (comment: only amino acid auxotrophic cell) ------- COMMENT: 7d86a078e24b65b7 2 PUgpLk0nFPEcKt8na2EJceBopRk (comment: storage) ------- COMMENT: 7d86a078e24b65b7 3 oIrfCjpBSH9bG4y2ZvCQY+7C3q4 In the presence of elevated Zn2 levels, however, they were severely growth-inhibited (Fig. 1, A and B). ------- COMMENT: 7d86a078e24b65b7 4 nu/vRNENr0zWyWjZrCHs6js0rDM zhf strain were viable and showed only a minor reduction in growth rate under control conditions without any added heavy metal salts. ------- COMMENT: 7d86a078e24b65b7 5 FK6NeGsHNjV26tD4+SPw58ZbQe4 Similarly, the absence of a functional Zhf protein rendered the S. pombe cells Co2-hypersensitive (Fig. 1C). At 1 mM Co2 in EMM, wild-type cells showed 94% (11%) of the optical density of untreated control cells, whereas zhf cells reached only 36% (3.5%). ------- COMMENT: 7d86a078e24b65b7 6 v5f5FuRmFeMX6ckiwi4ip5CIlGA In contrast to that, the zhf disruption was found to significantly protect cells from toxicity of Cd2 ions, the third known substrate of CDF proteins. At the IC50 con- centration for wild-type cells of 100 M, zhf cells were inhib- ited by only 4% (3%) (Fig. 1D). ------- COMMENT: 7d86a078e24b65b7 7 YCWnd1pvR19qx/wm5FBW54h09Xk a pronounced protective effect of the Zhf inactivation could be detected also for Ni2 ------- COMMENT: 7d86a078e24b65b7 8 sIQGBUB/ybsYlnskjIZFWcc/4Js To determine whether the main pathway for Cd2 de- toxification in S. pombe, the formation of phytochelatins, is required for the zhf-dependent protection, the zhf gene was disrupted in the pcs strain Sp27 (20). Toxicity assays showed that the protective effect was even more pronounced in this genetic background. ------- COMMENT: 7d8eddd77bc43a59 20 CWjnqxzLI3uV7XUvFms9+R1QUdE We found that Nsk1 localizes pre- dominantly in the nucleolus during interphase, as judged by colo- calization with fission yeast fibrillarin, Fib1 (Beauregard et al., 2009; Figures 2A and S3A). ------- COMMENT: 7d8eddd77bc43a59 21 1RONM5G0b5l1EPpIwkPVUGPF140 During prometaphase and metaphase, Nsk1 localizes broadly throughout the nucleoplasm, suggesting it is re- leased from the nucleolus at the G2/M transition (Figure 2A). ------- COMMENT: 7d8eddd77bc43a59 23 WO+bc9KqG9cxFQeiSSbvyU7SMC8 During anaphase B, Nsk1 appears as two prominent dots that seem to co- localize with SPBs, before it returns to the nucleolus at the end of anaphase B, indicating that Nsk1 undergoes cell cycle–dependent changes in its localization (Figure 2A). (vw Plus that it is at the SPB-kinetochore interface) ------- COMMENT: 7d8eddd77bc43a59 27 sjrRgVWr2cfI6P1/zulEeBy9pOg To our surprise, although loss of Msd1 or Alp7 had little or no effect on Nsk1 localization, we were unable to observe Nsk1 at the spindle pole in the absence of Dlc1 in fixed-cell preparations (Figure 6A). ------- COMMENT: 7d8eddd77bc43a59 31 01oGtk1smHLVHYQjmnrfPZYYhj8 This strongly suggests that Nsk1 is required for accurate chromosome segrega- tion, promoting the tethering of kinetochores to SPBs during ana- phase B (see Discussion). In 9.0% of interphase Δnsk1 cells, one pair of sister kinetochores failed to cluster near the SPB during interphase (Figure 7A). ------- COMMENT: 7d8eddd77bc43a59 33 csK7z6GFlsmvFkMNhF6y4vUxvsk In 9.0% of interphase Δnsk1 cells, one pair of sister kinetochores failed to cluster near the SPB during interphase (Figure 7A). ------- COMMENT: 7d8eddd77bc43a59 35 OuqASzt7LWerM5jIwz+u8OtfiBI dis2 is required for the retreival of unclustered kinetochores in nsk delete (additive chromosome segregation defects) ------- COMMENT: 7d8eddd77bc43a59 36 NJzyVcGmQTIpNhwQTAz6mOaxLUE To test whether Nsk1 is dephosphorylated by the proline-directed Cdc14- like phosphatase Clp1, cell cycle–dependent modification of Nsk1 was examined in nda3-KM311 nsk1-gfp clp1(C286S) cells, which express a substrate-trapping allele of Clp1 that binds, but does not release, its phosphosubstrates (Jia et al., 1995; Chen et al., 2008). In these cells, the proportion of faster-migrating Nsk1 increases only very slowly after release into anaphase, suggesting that Clp1 dephosphorylates Nsk1 (Figure 5, A and B). Consistent with this, we found that Clp1(C286S) coimmunoprecipitates with Nsk1 from cell lysates (Figure 5C) ------- COMMENT: 7d8eddd77bc43a59 37 NJzyVcGmQTIpNhwQTAz6mOaxLUE To test whether Nsk1 is dephosphorylated by the proline-directed Cdc14- like phosphatase Clp1, cell cycle–dependent modification of Nsk1 was examined in nda3-KM311 nsk1-gfp clp1(C286S) cells, which express a substrate-trapping allele of Clp1 that binds, but does not release, its phosphosubstrates (Jia et al., 1995; Chen et al., 2008). In these cells, the proportion of faster-migrating Nsk1 increases only very slowly after release into anaphase, suggesting that Clp1 dephosphorylates Nsk1 (Figure 5, A and B). Consistent with this, we found that Clp1(C286S) coimmunoprecipitates with Nsk1 from cell lysates (Figure 5C) ------- COMMENT: 7d8eddd77bc43a59 38 He75xToeN30Nxc5ymbo2KXnhkAY In the absence of Nsk1, some kinetochores become detached from spindle poles during anaphase B ------- COMMENT: 7d8eddd77bc43a59 39 7TFojaKU9mZJK+FNRnny2Rk93eE Since Δnsk1 Δdis2 double mutants displayed mitotic abnormalities, we chose Nsk1 for further analysis (Figure 1B). ------- COMMENT: 7d8eddd77bc43a59 40 Cc9mmPJnTjqM/T2sKN6tCTtUnYM We found that both Δnsk1 and Δdis2 cells are delayed in the timing of anaphase onset and that this effect is exacerbated in Δnsk1 Δdis2 double mutants (Figure 1D). ------- COMMENT: 7d8eddd77bc43a59 41 Cc9mmPJnTjqM/T2sKN6tCTtUnYM We found that both Δnsk1 and Δdis2 cells are delayed in the timing of anaphase onset and that this effect is exacerbated in Δnsk1 Δdis2 double mutants (Figure 1D). ------- COMMENT: 7d8eddd77bc43a59 42 Cc9mmPJnTjqM/T2sKN6tCTtUnYM We found that both Δnsk1 and Δdis2 cells are delayed in the timing of anaphase onset and that this effect is exacerbated in Δnsk1 Δdis2 double mutants (Figure 1D). ------- COMMENT: 7d8eddd77bc43a59 43 1T8WvxSbWegRlO4BAdqdBKXOhE4 Nevertheless, we found that both Δnsk1 and Δdis2 cells missegregate chromosome 1, and that this is exacerbated in Δnsk1 Δdis2 double mutants (Figure 1C). ------- COMMENT: 7d8eddd77bc43a59 44 1T8WvxSbWegRlO4BAdqdBKXOhE4 Nevertheless, we found that both Δnsk1 and Δdis2 cells missegregate chromosome 1, and that this is exacerbated in Δnsk1 Δdis2 double mutants (Figure 1C). ------- COMMENT: 7d8eddd77bc43a59 45 6pzgWvM9lSBTLEk8PB1wThdlUnc The delay in anaphase onset observed in Δnsk1 cells is abolished by deletion of either mad2 or mad3, indicating that this is due either to activation of, or failure to silence, the SAC (Figure 1E). ------- COMMENT: 7d8eddd77bc43a59 46 6pzgWvM9lSBTLEk8PB1wThdlUnc The delay in anaphase onset observed in Δnsk1 cells is abolished by deletion of either mad2 or mad3, indicating that this is due either to activation of, or failure to silence, the SAC (Figure 1E). ------- COMMENT: 7d8eddd77bc43a59 47 HwKiwTjQpNxepgSQh+x5MAx+vF0 To distinguish between these possibilities, we moni- tored Cdc13 (Cyclin B) destruction in single cells following chemical inactivation of Ark1 (Aurora B) kinase in mitotically arrested nda3- KM311 cells. The nda3-KM311 allele encodes a cold-sensitive β-tubulin protein that causes cells to arrest in mitosis at 18°C with no microtubules (Hiraoka et al., 1984). We found that loss of nsk1 had no effect in this assay, indicating that Nsk1 is not required to silence the SAC (Figure S2A). ------- COMMENT: 7d8eddd77bc43a59 48 CvZV2rO3QdEejyOzJBK6F1pb1bc (Figure S2A). ------- COMMENT: 7d8eddd77bc43a59 49 r7EFuAsc9eIj1VcGRIUQI7CvTxY Indeed, we found that Δdis2, but not Δnsk1, mutants suppress the proliferation defect of a temperature-sensitive ark1-T7 mutant, indicating that Nsk1 does not counteract Ark1 function (Figure S2B) ------- COMMENT: 7d8eddd77bc43a59 50 r7EFuAsc9eIj1VcGRIUQI7CvTxY Indeed, we found that Δdis2, but not Δnsk1, mutants suppress the proliferation defect of a temperature-sensitive ark1-T7 mutant, indicating that Nsk1 does not counteract Ark1 function (Figure S2B) ------- COMMENT: 7d8eddd77bc43a59 51 r7EFuAsc9eIj1VcGRIUQI7CvTxY Indeed, we found that Δdis2, but not Δnsk1, mutants suppress the proliferation defect of a temperature-sensitive ark1-T7 mutant, indicating that Nsk1 does not counteract Ark1 function (Figure S2B) ------- COMMENT: 7d8eddd77bc43a59 52 sahMZTbg7y/CzlEJkjGuiK/nB9M Nevertheless, the chromosome loss rate was considerably worse in Δnsk1 Δmad2 cells than in Δnsk1 single mu- tant (Table 1). ------- COMMENT: 7d8eddd77bc43a59 53 sahMZTbg7y/CzlEJkjGuiK/nB9M Nevertheless, the chromosome loss rate was considerably worse in Δnsk1 Δmad2 cells than in Δnsk1 single mu- tant (Table 1). ------- COMMENT: 7d8eddd77bc43a59 54 xHjZ53eA6f+7UZaoVLP6C1Fuh8g (Table 1). ------- COMMENT: 7d8eddd77bc43a59 55 xHjZ53eA6f+7UZaoVLP6C1Fuh8g (Table 1). ------- COMMENT: 7d8eddd77bc43a59 56 sahMZTbg7y/CzlEJkjGuiK/nB9M Nevertheless, the chromosome loss rate was considerably worse in Δnsk1 Δmad2 cells than in Δnsk1 single mu- tant (Table 1). ------- COMMENT: 7d8eddd77bc43a59 57 mg642HtBqNcU4/9a226pAsIoavI FIGURE 5: Dephosphorylation of Nsk1 by Clp1 promotes its association to the kinetochore–SPB junction. ------- COMMENT: 7d8eddd77bc43a59 58 jAz9ld7pVjgzscahg4rxm1T/1Ss These data indicate that Nsk1 is phosphorylated by Cdk1/Cdc2 kinase on multiple sites in early mitosis, and this prevents Nsk1 localization to the spindle and kinetochore–SPB interface until it is dephosphorylated by Clp1 at anaphase onset. ------- COMMENT: 7d8eddd77bc43a59 59 VTvG7N9yCbxeZkPrMUUfRg4HHY4 Similar results were obtained when kinetochore position was assessed with other GFP-tagged kinetochore proteins and in clp1(C286S) mutant, in which relocalization of Nsk1 to the kineto- chore–SPB junction is impaired (Figure S6A). ------- COMMENT: 7d8eddd77bc43a59 60 qrMAOHeX+Uvjym/DCSoXB7g4q+0 Whereas cells lacking Nsk1 dis- played no defect in kinetochore retrieval, cells lacking either Dis2 or Dam1 were substantially impaired (Figure 8D). ------- COMMENT: 7da922bfc1ae22b3 1 9E26J2JjW1ZsIOTGwfqolUqXwd4 Cdc12 pulls down only 27% as much Cdc15 in a similar block and release experiment (Supp. Fig. 1B) ------- COMMENT: 7da99c40d10d0680 16 zYG3T4cvmLOFzfEflKV8ZiNKc7A (comment: mah: deleted this extension because it refers to a pseudogene: annotation_extension=assayed_using(PomBase:SPBPB10D8.03)) ------- COMMENT: 7db555db2a36a8e7 3 AX5dYFgK8yQXAgv0XXyDync06/M (comment: Also related to FYPO:0002822 for alp6) ------- COMMENT: 7db555db2a36a8e7 21 389kqiIjaLSNsE72n49O8KsJaHU (comment: CHECK monopolar spindle formation) ------- COMMENT: 7db555db2a36a8e7 22 389kqiIjaLSNsE72n49O8KsJaHU (comment: CHECK monopolar spindle formation) ------- COMMENT: 7db555db2a36a8e7 23 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 7db555db2a36a8e7 24 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7db555db2a36a8e7 25 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7db555db2a36a8e7 26 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7db555db2a36a8e7 27 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7db555db2a36a8e7 28 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7db555db2a36a8e7 29 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7db555db2a36a8e7 30 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7db555db2a36a8e7 31 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7db555db2a36a8e7 32 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7db555db2a36a8e7 33 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7db555db2a36a8e7 34 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7db555db2a36a8e7 35 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7db555db2a36a8e7 36 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7db555db2a36a8e7 37 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7db555db2a36a8e7 38 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7db555db2a36a8e7 39 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7db555db2a36a8e7 40 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7db555db2a36a8e7 42 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 7db555db2a36a8e7 43 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7db555db2a36a8e7 44 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7db555db2a36a8e7 45 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 7db555db2a36a8e7 46 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 7db555db2a36a8e7 47 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 7db555db2a36a8e7 48 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 7db555db2a36a8e7 49 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 7db555db2a36a8e7 50 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 7db555db2a36a8e7 51 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 7db555db2a36a8e7 52 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 7db555db2a36a8e7 56 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 7db555db2a36a8e7 57 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 7dcc48c984dda0bd 5 5DWqo03puFeQtodWqcCbmJ6nfyM fig 1A ------- COMMENT: 7dcc48c984dda0bd 6 JNgSLxGLb23ybSyBetITezKI9as fig 1C/D ------- COMMENT: 7dcc48c984dda0bd 7 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 7dcc48c984dda0bd 8 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 7dcc48c984dda0bd 9 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 7dcc48c984dda0bd 10 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 7dcc48c984dda0bd 11 zvy6HVkPUGt0msFBfOM8j5Xwy2Q Fig. 2C/D ------- COMMENT: 7dcc48c984dda0bd 12 zvy6HVkPUGt0msFBfOM8j5Xwy2Q Fig. 2C/D ------- COMMENT: 7dcc48c984dda0bd 13 zvy6HVkPUGt0msFBfOM8j5Xwy2Q Fig. 2C/D ------- COMMENT: 7dcc48c984dda0bd 14 zvy6HVkPUGt0msFBfOM8j5Xwy2Q Fig. 2C/D ------- COMMENT: 7dcc48c984dda0bd 15 ZFFCy3C/1OLrEfwM/r1FU7Mfuvw fig 2E/F ------- COMMENT: 7dcc48c984dda0bd 16 ZFFCy3C/1OLrEfwM/r1FU7Mfuvw fig 2E/F ------- COMMENT: 7dcc48c984dda0bd 17 ZFFCy3C/1OLrEfwM/r1FU7Mfuvw fig 2E/F ------- COMMENT: 7dcc48c984dda0bd 18 ZFFCy3C/1OLrEfwM/r1FU7Mfuvw fig 2E/F ------- COMMENT: 7dcc48c984dda0bd 19 WR4eY9sTthO3PnzjKXFbZFO6hx0 figure 3D ------- COMMENT: 7dcc48c984dda0bd 20 zvy6HVkPUGt0msFBfOM8j5Xwy2Q Fig. 2C/D ------- COMMENT: 7dcc48c984dda0bd 21 WR4eY9sTthO3PnzjKXFbZFO6hx0 figure 3D ------- COMMENT: 7dcc48c984dda0bd 22 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 7dcc48c984dda0bd 23 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 7dcc48c984dda0bd 24 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 7dcc48c984dda0bd 25 /BZjPAvUyZcRggNcLIb+lQAvvFI table2 ------- COMMENT: 7dcc48c984dda0bd 26 /BZjPAvUyZcRggNcLIb+lQAvvFI table2 ------- COMMENT: 7dcc48c984dda0bd 27 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 7dda0b52c4bd8bfa 21 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 7dda0b52c4bd8bfa 24 jgPxh0S8rof4lSz1TG30wnuWFWk Figure 4e ------- COMMENT: 7dda0b52c4bd8bfa 25 jgPxh0S8rof4lSz1TG30wnuWFWk Figure 4e ------- COMMENT: 7dda0b52c4bd8bfa 36 eWbxVFB5JTCpritU2Gd/1qI/pvg Ste9 was indispensable for the growth of the wee1 cells, which had to lengthen the pre-Start G1 period to restrain DNA synthesis until the critical size to override Start control was attained....Ste9 might be required for main- tenance of the Cdc2 kinase in a pre-Start form, suggested by the fact that overexpression of Ste9 in- duced rereplication of the genome due to reduction of the mitotic kinase activity of the Cdc13/Cdc2 com- plex, and rereplication in the cdc2ts strain was pre- vented by the ste9 mutation. ------- COMMENT: 7dda9fd3efb63699 9 3WTSfD6hDCwHmt04B9hR6GhWqYk (comment: also assayed using bulk histones from calf thymus) ------- COMMENT: 7dda9fd3efb63699 10 3WTSfD6hDCwHmt04B9hR6GhWqYk (comment: also assayed using bulk histones from calf thymus) ------- COMMENT: 7e0c631c21160a2d 12 nubF1IZITGlWNGFgsBJ+5oaxpbE (comment: inferred from FYPO:0000825, FYPO:0001117, FYPO:0005743, FYPO:0007674 phenotypes (including conditions)) ------- COMMENT: 7e2a2611a978753c 1 7Ub1+CI9H9KZmMOQfFysIkJm17c Fig. 1C ------- COMMENT: 7e2a2611a978753c 2 7Ub1+CI9H9KZmMOQfFysIkJm17c Fig. 1C ------- COMMENT: 7e2a2611a978753c 3 7Ub1+CI9H9KZmMOQfFysIkJm17c Fig. 1C ------- COMMENT: 7e2a2611a978753c 4 7Ub1+CI9H9KZmMOQfFysIkJm17c Fig. 1C ------- COMMENT: 7e2a2611a978753c 5 jgoGuBgb+Ot76esr6oM9CSFJl3I Fig 1E ------- COMMENT: 7e2a2611a978753c 6 jgoGuBgb+Ot76esr6oM9CSFJl3I Fig 1E ------- COMMENT: 7e2a2611a978753c 7 7Ub1+CI9H9KZmMOQfFysIkJm17c Fig. 1C ------- COMMENT: 7e2a2611a978753c 9 7C4v+NWOowf/qEK/lmsSvW4+ADs Fig. 1C, Fig3 ------- COMMENT: 7e2a2611a978753c 11 EmurKrrLioI2sbzAAEaMCU9NGzo Fig. 4D ------- COMMENT: 7e2a2611a978753c 12 EmurKrrLioI2sbzAAEaMCU9NGzo Fig. 4D ------- COMMENT: 7e2a2611a978753c 15 Hs6/5ONBSV4p6V72kaqANUGhVX4 fig 4F ------- COMMENT: 7e2a2611a978753c 16 Hs6/5ONBSV4p6V72kaqANUGhVX4 fig 4F ------- COMMENT: 7e2a2611a978753c 17 Hs6/5ONBSV4p6V72kaqANUGhVX4 fig 4F ------- COMMENT: 7e2a2611a978753c 18 Hs6/5ONBSV4p6V72kaqANUGhVX4 fig 4F ------- COMMENT: 7e2a2611a978753c 19 jXSWdd6gLBYKGs46rmwvGU0692M (G2) Fig. 6B ------- COMMENT: 7e2a2611a978753c 20 g+6KsSh7KXgwLI/OlQhwLpBudG0 Fig. 6B (comment: CHECK G2) ------- COMMENT: 7e2a2611a978753c 21 v74p86ZyTl9JdH15BhfWqT3EBjc Fig. 6C (comment: CHECK G2) ------- COMMENT: 7e2a2611a978753c 22 xrTmEWzY6RJdOU8Tzjvostj3pEs fig 6F ------- COMMENT: 7e2a2611a978753c 23 xrTmEWzY6RJdOU8Tzjvostj3pEs fig 6F ------- COMMENT: 7e2a2611a978753c 24 xrTmEWzY6RJdOU8Tzjvostj3pEs fig 6F ------- COMMENT: 7e66641cb8bf60c5 1 aeIrnWSoZxVYN6XBhr6mSpq6F6I (comment: assayed substrate: myelin basic protein; assayed enzyme is, or is bound to, Pmo25) ------- COMMENT: 7e66641cb8bf60c5 2 aeIrnWSoZxVYN6XBhr6mSpq6F6I (comment: assayed substrate: myelin basic protein; assayed enzyme is, or is bound to, Pmo25) ------- COMMENT: 7e66641cb8bf60c5 3 aeIrnWSoZxVYN6XBhr6mSpq6F6I (comment: assayed substrate: myelin basic protein; assayed enzyme is, or is bound to, Pmo25) ------- COMMENT: 7e66641cb8bf60c5 4 aeIrnWSoZxVYN6XBhr6mSpq6F6I (comment: assayed substrate: myelin basic protein; assayed enzyme is, or is bound to, Pmo25) ------- COMMENT: 7e66641cb8bf60c5 5 aeIrnWSoZxVYN6XBhr6mSpq6F6I (comment: assayed substrate: myelin basic protein; assayed enzyme is, or is bound to, Pmo25) ------- COMMENT: 7e66641cb8bf60c5 6 aeIrnWSoZxVYN6XBhr6mSpq6F6I (comment: assayed substrate: myelin basic protein; assayed enzyme is, or is bound to, Pmo25) ------- COMMENT: 7e7c2c9e26201c7d 10 Rdt406mHEMbid6fma7an+LmOdhE (comment: evidence is combination of ChIP in this paper plus data in other publications showing that Rad50 orthologs (and therefore almost certainly Sc Rad50) binds DNA directl) ------- COMMENT: 7e7c2c9e26201c7d 11 EzXdPPZZm05Cc2hbvJQqyV4/ovI (comment: evidence is combination of ChIP in this paper plus data in other publications showing that Rad52 binds DNA directly) ------- COMMENT: 7e7c2c9e26201c7d 12 806FS203qyMIWwV3jsARCqBcvww (comment: evidence is combination of ChIP in this paper plus data in other publications showing that Ctp1 binds DNA directly) ------- COMMENT: 7e7d155c2e0f6d6b 1 VaXiSZZO7R52KMDhEepRtSmtYgA Fig.1 ------- COMMENT: 7e7d155c2e0f6d6b 2 VaXiSZZO7R52KMDhEepRtSmtYgA Fig.1 ------- COMMENT: 7e7d155c2e0f6d6b 3 VaXiSZZO7R52KMDhEepRtSmtYgA Fig.1 ------- COMMENT: 7e7d155c2e0f6d6b 4 VaXiSZZO7R52KMDhEepRtSmtYgA Fig.1 ------- COMMENT: 7e7d155c2e0f6d6b 5 VaXiSZZO7R52KMDhEepRtSmtYgA Fig.1 ------- COMMENT: 7e7d155c2e0f6d6b 6 VaXiSZZO7R52KMDhEepRtSmtYgA Fig.1 ------- COMMENT: 7e7d155c2e0f6d6b 7 VaXiSZZO7R52KMDhEepRtSmtYgA Fig.1 ------- COMMENT: 7e7d155c2e0f6d6b 8 VaXiSZZO7R52KMDhEepRtSmtYgA Fig.1 ------- COMMENT: 7e7d155c2e0f6d6b 9 VaXiSZZO7R52KMDhEepRtSmtYgA Fig.1 ------- COMMENT: 7e7d155c2e0f6d6b 10 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 11 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 12 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 13 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 14 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 15 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 16 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 17 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 18 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 19 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 20 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 21 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 22 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 23 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 24 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 25 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 26 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 27 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 28 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 29 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 30 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 31 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 32 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 33 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 34 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 35 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 36 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 37 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 38 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 39 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 40 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 41 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 42 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 43 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 44 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 45 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 46 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 47 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 48 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 49 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 50 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e7d155c2e0f6d6b 51 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 7e83e3410ced8531 5 hIhcVkZ2wSA///OLSjScMLMuYIc (comment: greater range of lengths) ------- COMMENT: 7e83e3410ced8531 6 hIhcVkZ2wSA///OLSjScMLMuYIc (comment: greater range of lengths) ------- COMMENT: 7e83e3410ced8531 7 hIhcVkZ2wSA///OLSjScMLMuYIc (comment: greater range of lengths) ------- COMMENT: 7e83e3410ced8531 14 psYW5bzGkuqI8VXIek1PY0YjKWM (comment: WT 3%) ------- COMMENT: 7e83e3410ced8531 15 psYW5bzGkuqI8VXIek1PY0YjKWM (comment: WT 3%) ------- COMMENT: 7e83e3410ced8531 16 psYW5bzGkuqI8VXIek1PY0YjKWM (comment: WT 3%) ------- COMMENT: 7e83e3410ced8531 46 psYW5bzGkuqI8VXIek1PY0YjKWM (comment: WT 3%) ------- COMMENT: 7e83e3410ced8531 50 v1zR0ETyaOby9jj/mDo1M95M7kE fig 3A ------- COMMENT: 7e8ed360ac228b68 1 Am0kjmUysRAWL/4jN3S5VzotyGw Figure 1a ------- COMMENT: 7e8ed360ac228b68 2 MB8HfFxX5AmOgwbeZDObRjaHiEY Figure 1b ------- COMMENT: 7e8ed360ac228b68 3 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 7e8ed360ac228b68 4 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 7e8ed360ac228b68 5 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 7e8ed360ac228b68 6 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 7e8ed360ac228b68 7 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 7e8ed360ac228b68 8 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 7e8ed360ac228b68 9 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 7e8ed360ac228b68 10 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 7e8ed360ac228b68 11 ky/+0122BdU7xvxUw6twS2LfsEw Figure 3a ------- COMMENT: 7e8ed360ac228b68 12 ky/+0122BdU7xvxUw6twS2LfsEw Figure 3a ------- COMMENT: 7e8ed360ac228b68 13 ky/+0122BdU7xvxUw6twS2LfsEw Figure 3a ------- COMMENT: 7e8ed360ac228b68 14 ky/+0122BdU7xvxUw6twS2LfsEw Figure 3a ------- COMMENT: 7e8ed360ac228b68 15 V5KMiVezUnja/RciehCCfRfe1y0 Figure 3b ------- COMMENT: 7e8ed360ac228b68 16 V5KMiVezUnja/RciehCCfRfe1y0 Figure 3b ------- COMMENT: 7e8ed360ac228b68 17 V5KMiVezUnja/RciehCCfRfe1y0 Figure 3b ------- COMMENT: 7e8ed360ac228b68 18 V5KMiVezUnja/RciehCCfRfe1y0 Figure 3b ------- COMMENT: 7e8ed360ac228b68 19 FnI8kzviS6kGF4O7mHuCSZyPdg4 Figure 3c ------- COMMENT: 7e8ed360ac228b68 20 FnI8kzviS6kGF4O7mHuCSZyPdg4 Figure 3c ------- COMMENT: 7e8ed360ac228b68 21 FnI8kzviS6kGF4O7mHuCSZyPdg4 Figure 3c ------- COMMENT: 7e8ed360ac228b68 22 FnI8kzviS6kGF4O7mHuCSZyPdg4 Figure 3c ------- COMMENT: 7e8ed360ac228b68 23 3pioZFSOwSBdtd9rsLGb0VgguUc Figure 3c, rst2∆ partially rescues the pps1∆ pka1∆ strain on KCl ------- COMMENT: 7e8ed360ac228b68 24 PExxPdSJXlfl9M5u1zX3PyeLfU4 Figure 3c, rst2∆ partially rescues the pps1∆ pka1∆ strain on CaCl2 ------- COMMENT: 7e8ed360ac228b68 25 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 7e8ed360ac228b68 26 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 7e8ed360ac228b68 27 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 7e8ed360ac228b68 28 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 7e8ed360ac228b68 29 p+oVe0jJEd3iUOZ6+ONJVCdjeM4 Figure 4a ------- COMMENT: 7e8ed360ac228b68 30 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 7e8ed360ac228b68 31 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 7e8ed360ac228b68 32 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 7e8ed360ac228b68 33 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 7e8ed360ac228b68 34 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 7e8ed360ac228b68 35 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 7e8ed360ac228b68 36 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 7e8ed360ac228b68 37 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 7e8ed360ac228b68 38 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 7e8ed360ac228b68 39 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 7e8ed360ac228b68 40 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 7e8ed360ac228b68 41 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 7e8ed360ac228b68 42 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 7e8ed360ac228b68 43 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 7e8ed360ac228b68 44 p+oVe0jJEd3iUOZ6+ONJVCdjeM4 Figure 4a ------- COMMENT: 7e8ed360ac228b68 45 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 7e8ed360ac228b68 46 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 7e8ed360ac228b68 47 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 7e8ed360ac228b68 48 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 7e8ed360ac228b68 49 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 7e8ed360ac228b68 50 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 7e8ed360ac228b68 51 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 7e8ed360ac228b68 52 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 7e8ed360ac228b68 53 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 7e8ed360ac228b68 54 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 7e8ed360ac228b68 55 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 7e8ed360ac228b68 56 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 7e8ed360ac228b68 57 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 7ea26987e7982227 1 BKf8VbIhcT9iwQOLIxS2chrjtnE Figure 1, C and D restrictive temperature mutant. ------- COMMENT: 7ea26987e7982227 2 FUT8pwvxzsItGu1EiT3PIUJOAY4 (comment: recruits glucanases and glucan synthases to division site) ------- COMMENT: 7ea26987e7982227 6 Ndg6+iBaB6qy/c5DeHLFkX3/YGg Supplemental Figure S1A (comment: Smi1 was more abundant in pull downs of cells express- ing GFP-Bgs4 than in those from GFP-Bgs1) ------- COMMENT: 7ea26987e7982227 8 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 7ea26987e7982227 10 vwnYpS/CMrPjtCsAufVv567v2f0 We confirmed the defects in septa ....... In cells with closed septa, the primary septum was uneven (Figure 3, red arrows) and thinner in smi1-1 cells than in WT (Figure 3A). ------- COMMENT: 7ea26987e7982227 17 C6hMMGicPZZZ/BLDfPl+G7OyQ6M (comment: CHECK see above) ------- COMMENT: 7ea26987e7982227 18 WrwMeuIgondmqDRf7Y9y1DzpB6w (comment: CONDITION 36 degrees) ------- COMMENT: 7ea26987e7982227 19 WrwMeuIgondmqDRf7Y9y1DzpB6w (comment: CONDITION 36 degrees) ------- COMMENT: 7ea26987e7982227 20 WrwMeuIgondmqDRf7Y9y1DzpB6w (comment: CONDITION 36 degrees) ------- COMMENT: 7ea26987e7982227 21 WrwMeuIgondmqDRf7Y9y1DzpB6w (comment: CONDITION 36 degrees) ------- COMMENT: 7ea26987e7982227 22 WrwMeuIgondmqDRf7Y9y1DzpB6w (comment: CONDITION 36 degrees) ------- COMMENT: 7ea26987e7982227 24 WrwMeuIgondmqDRf7Y9y1DzpB6w (comment: CONDITION 36 degrees) ------- COMMENT: 7ea26987e7982227 26 Lips/k+YNFbai3LqvoKx4BXpItU Supplemental Figure S2, A–D ------- COMMENT: 7ea26987e7982227 27 Lips/k+YNFbai3LqvoKx4BXpItU Supplemental Figure S2, A–D ------- COMMENT: 7ea26987e7982227 30 WrwMeuIgondmqDRf7Y9y1DzpB6w (comment: CONDITION 36 degrees) ------- COMMENT: 7ea26987e7982227 31 WrwMeuIgondmqDRf7Y9y1DzpB6w (comment: CONDITION 36 degrees) ------- COMMENT: 7ea26987e7982227 33 WrwMeuIgondmqDRf7Y9y1DzpB6w (comment: CONDITION 36 degrees) ------- COMMENT: 7ea26987e7982227 34 WrwMeuIgondmqDRf7Y9y1DzpB6w (comment: CONDITION 36 degrees) ------- COMMENT: 7ea26987e7982227 35 3hdHUQOVgJfW9POdSinv9BUafr8 (comment: CHECK smi1-1 bgs1-191 and bgs1-D277N) ------- COMMENT: 7ea26987e7982227 36 Af808samZZiHXclAKh8R/qdPAmc (comment: CHECK smi1-1 cwg1-1 and cwg1-2) ------- COMMENT: 7ea26987e7982227 37 98Ua0Lcsx0tUvWWSU5Ix0ssCBn8 (comment: CHECK pck1Δ) ------- COMMENT: 7ea26987e7982227 38 dBs/5fzwvMvvP1k3oJSAbksknqQ (comment: CHECK pck2-Δ1) ------- COMMENT: 7ea26987e7982227 39 HS1183DYIji2KmzxpgNmJIzu9mw (comment: CHECK rga7Δ) ------- COMMENT: 7ea26987e7982227 40 zMX1TdG7waY2/DgZX5bJtghISyo (comment: CHECK rng10Δ) ------- COMMENT: 7ea26987e7982227 42 aF8jJjYBomJR6yHb7Oavv8gVxRU (comment: CHECK mok1-664) ------- COMMENT: 7ea26987e7982227 43 YIjVajbCHJ6ST9GQe810XAhMYeI (comment: CHECK art1Δ) ------- COMMENT: 7ea26987e7982227 44 9I17f6rr3TQ4EheqhHEn4fu5y6c (comment: CHECK mob1-M17) ------- COMMENT: 7ea26987e7982227 45 2x6eoEbcTAdD8/yKrm+3j5ZVOO0 (comment: CHECK ync13-4 (mild interactin)() ------- COMMENT: 7ea26987e7982227 47 cCQMO4UwQKkjq//Y2C87L++Wzps (comment: CHECK 38.5% cf WT 11.5%) Figure 1F ------- COMMENT: 7ea26987e7982227 48 FUT8pwvxzsItGu1EiT3PIUJOAY4 recruits glucanases and glucan synthases to division site ------- COMMENT: 7ea26987e7982227 49 9VMohh/STRT9xZQYj0MMjXM8y2Q Together, these results confirmed that Sbg1 is specific to Bgs1, while Smi1 regulates the levels of both Bgs4 and Bgs1 at the division site, with a more important role for Bgs4 (Figure 7, A–C). ------- COMMENT: 7eac5353ab8b141d 1 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 7eac5353ab8b141d 2 2PILl4QpQTRfb9rYJPm6XFi/Rp4 As expected from the two-hybrid results, neither the U-box (His6-Prp19p 1–58) nor the C terminus containing the WD40 repeats (His6-Prp19p 165–503) was able to tetramerize (data not shown). ------- COMMENT: 7eac5353ab8b141d 4 2PILl4QpQTRfb9rYJPm6XFi/Rp4 As expected from the two-hybrid results, neither the U-box (His6-Prp19p 1–58) nor the C terminus containing the WD40 repeats (His6-Prp19p 165–503) was able to tetramerize (data not shown). ------- COMMENT: 7eac5353ab8b141d 5 2PILl4QpQTRfb9rYJPm6XFi/Rp4 As expected from the two-hybrid results, neither the U-box (His6-Prp19p 1–58) nor the C terminus containing the WD40 repeats (His6-Prp19p 165–503) was able to tetramerize (data not shown). ------- COMMENT: 7f0d3ce30ca3af6f 6 p23VaE2MmzhdxFHAPnFMA17hdOk Fig. 2A and B ------- COMMENT: 7f0d3ce30ca3af6f 7 p23VaE2MmzhdxFHAPnFMA17hdOk Fig. 2A and B ------- COMMENT: 7f0d3ce30ca3af6f 8 p23VaE2MmzhdxFHAPnFMA17hdOk Fig. 2A and B ------- COMMENT: 7f0d3ce30ca3af6f 9 p23VaE2MmzhdxFHAPnFMA17hdOk Fig. 2A and B ------- COMMENT: 7f0d3ce30ca3af6f 10 p23VaE2MmzhdxFHAPnFMA17hdOk Fig. 2A and B ------- COMMENT: 7f17aec8a060844d 1 u1Jns5dAs9WRl44AZEGl0xCCD34 Failure of NE fenestration during mitosis in the double tts1del cut1-6 mutant ------- COMMENT: 7f17aec8a060844d 37 Bp0GqPRux3q5VxGinjpmM6IvZ8Y ------- COMMENT: 7f89392aaf70c882 17 iypPNmshYFGQah+VqNgmGKwPEik (comment: CHECK during meiosis) ------- COMMENT: 7f8b3d85a47ad977 6 3R5wL0RvnSF3VYswLOUj4LV+zXw (comment: CHECK low penetrance) ------- COMMENT: 7f8b3d85a47ad977 7 3R5wL0RvnSF3VYswLOUj4LV+zXw (comment: CHECK low penetrance) ------- COMMENT: 7f8b3d85a47ad977 8 PBJWKWKnYbf/9sQJsgPw2YYz1cw large fractions of both abnormally long and abnormally short cells are present in the population ------- COMMENT: 7f8b3d85a47ad977 17 bUc32oU9ITX2O0znm/rpH2KtYeU ------- COMMENT: 7f8b3d85a47ad977 20 9xaJLLpuRkvyfRxNZEa0ahgOOm8 determined by expression microarrays from cbf11 knock-out cells growing exponentially in YES. targets: SPAC22A12.06c, ptl1, lcf1, lcf2, cut6, SPCC1281.06c ------- COMMENT: 7f8b3d85a47ad977 21 H8+VFdSwlnogoeDVSBl3MMM3w8Q determined by EMSA. Substrate: dsDNA oligonucleotide derived from promoters of cut6 and ptl1 genes (contain the CSL_response_element) ------- COMMENT: 7f8b3d85a47ad977 22 6G6xXeBdXRsK6yfTgOB4uJ76t9Q ChIP-seq and microarray data indicate that Cbf11 regulates lipid metabolism genes. ------- COMMENT: 7f8b3d85a47ad977 29 3R5wL0RvnSF3VYswLOUj4LV+zXw (comment: CHECK low penetrance) ------- COMMENT: 7f9fcf3e0233cf42 4 T7L8A3vGKezVIUWh99YvVOXa6d0 (comment: major) ------- COMMENT: 7fae84167ce708af 2 kYBdraOL4yWMe1yZ0f/pXZScgio (comment: ubiquitinated probably at K263) ------- COMMENT: 7fae84167ce708af 70 mBqoKLtV2R/gixlLBIkJZ2R3iK0 (comment: CHECK internalization abolished) ------- COMMENT: 7fae84167ce708af 71 bRZOB3quhlQAwsex4E8omD7+Pf4 (comment: CHECK internalization abolished) ------- COMMENT: 7fae84167ce708af 72 bRZOB3quhlQAwsex4E8omD7+Pf4 (comment: CHECK internalization abolished) ------- COMMENT: 7fae84167ce708af 73 I5Mq25RUudT+JmYXsBZsVg6Z8vU (comment: CHECK internalization abolished) ------- COMMENT: 7fae84167ce708af 83 /8PM4g/jqXgX9GGklQAsf4DqoUU (comment: CHECK have guessed at deleted residues) ------- COMMENT: 7fb114bb93891bd9 8 LDxHy+hn+f63l/2wIISPOrPcPGA (comment: CHECK splicing of artificial construct with wt or mutated splice sites assayed in mutants) ------- COMMENT: 7fc40d32cfb206ac 5 PUAvfk/yO9XiiX5xd/qMGbeLgeA mutant Cdc6 is not positively regulated by PCNA to the same extent as Cdc6+ ------- COMMENT: 7fc40d32cfb206ac 6 PUAvfk/yO9XiiX5xd/qMGbeLgeA mutant Cdc6 is not positively regulated by PCNA to the same extent as Cdc6+ ------- COMMENT: 7fecd247a7c91847 1 JIX1kQPtzpmnlKfkB4hIOYCjKAE As shown in Fig. 1A, its3-1 mutant cells could not grow at 33 °C, 36 °C, or in the YPD plate containing FK506, whereas wild-type cells grew normally. ------- COMMENT: 7fecd247a7c91847 2 JIX1kQPtzpmnlKfkB4hIOYCjKAE As shown in Fig. 1A, its3-1 mutant cells could not grow at 33 °C, 36 °C, or in the YPD plate containing FK506, whereas wild-type cells grew normally. ------- COMMENT: 7fecd247a7c91847 3 59TvY8voJrj92ZJkiMkZpLm1KUc PI(4)5K activity kinase: As shown in Fig. 1A, its3-1 mutant cells could not grow at 33 °C, 36 °C, or in the YPD plate containing FK506, whereas wild-type cells grew normally. ------- COMMENT: 7fecd247a7c91847 4 B6cxUr3jH2wois68DHcPJWyokDE Purified GST fusion pro- teins were subjected to in vitro kinase reaction as described under “Experimental Procedures.” Fig. 6 shows that mutant Its3 tagged with GST had detectable but reduced PI(4)5K ac- tivity compared with the wild-type. ------- COMMENT: 7fecd247a7c91847 5 IbObJE3YugpC4KEaOLuFtb0fDvY 􏰇ppb1 (􏰇CN in Fig. 2) mutant. As expected, no double mutant was obtained, indicating that its3 mutation and calcineurin deletion was synthetically lethal. ------- COMMENT: 7fecd247a7c91847 6 SKV2GaKg4X3hTcjacZ5ZyDYDYT0 Tetrad analysis of the heterozygous diploid showed two viable (Ura􏰄) and two inviable spores (Fig. 3D), indicating that the its3􏰀 gene is essential for cell growth. ------- COMMENT: 7fecd247a7c91847 7 iykECQVEkbZrmKgAwXLrMSDopCc (comment: CHECK NOT PLASMA MEMBRANE) As shown in Fig. 5B, its3-1 mutant cells contained about 10% of the amount of PI(4,5)P2 found in wild- type cells, indicating that the mutation caused a significant decrease in PI(4)P5K activity of Its3. ------- COMMENT: 7fecd247a7c91847 8 Ifj5KftPOis+uGDouYDYWp/lcGk Interestingly, the level of PI(4)P was significantly higher than that of the wild-type cells. ------- COMMENT: 7fecd247a7c91847 9 3SMVha64fwzEPN5W0YN9GKNUn0o (comment: CHECK NOT PLASMA MEMBRANE) ------- COMMENT: 7fecd247a7c91847 10 1HsWUMpdy7D4cqeC5CYL9jQaYTM In wild-type cells, a shift from 27 to 33 °C caused a transient heat-induced disorganization of actin patches, ------- COMMENT: 7fecd247a7c91847 11 kPxTFpRn0niPs03LQLsStsejWOk On the other hand, in the its3-1 mutant cells, actin patches were partially polarized at 27 °C, and the polarization was completely lost upon temperature upshift or FK506 treatment (Fig. 7A). ------- COMMENT: 7fecd247a7c91847 12 60KznKyWhartvzOBr2fzYlgJjnU Micro- scopic observation revealed that some mutant cells have a thick septum that was brightly stained with Calcofluor and was hardly seen in wild-type cells (Fig. 8A). ------- COMMENT: 7fecd247a7c91847 13 85vDydD6ti55tJm3a7bJKcw4bUI In addition, its3-1 mutant had a septation index approximately twice that seen in wild-type cells at the permissive temperature. ------- COMMENT: 7fecd247a7c91847 14 esNMWdKBdk7Afomz6Bev6BhJQbk GFP-Its3 localized to the plasma membrane at all stages of the cell cycle (Fig. 9A). ------- COMMENT: 7fecd247a7c91847 15 esNMWdKBdk7Afomz6Bev6BhJQbk GFP-Its3 localized to the plasma membrane at all stages of the cell cycle (Fig. 9A). ------- COMMENT: 7fecd247a7c91847 16 NURAh+Ky2Ze3sghFOiCcLSUuPss As shown in Fig. 9B, the GFP-Its3-1 mutant protein (GFP-mIts3) was no longer localized to the plasma membrane and instead... ------- COMMENT: 800a3d249c222a4d 1 T5AiOdAWcND8KshioIUsZ2HmZFw These results were also described in Hickey et al (H Levin lab) PMID: 26358720 ------- COMMENT: 809618c56c11583b 6 vcr+7qtELpi85citxIC3W+pDl8I fig 6B ------- COMMENT: 809618c56c11583b 7 vcr+7qtELpi85citxIC3W+pDl8I fig 6B ------- COMMENT: 809618c56c11583b 8 vcr+7qtELpi85citxIC3W+pDl8I fig 6B ------- COMMENT: 809618c56c11583b 9 vcr+7qtELpi85citxIC3W+pDl8I fig 6B ------- COMMENT: 80aa26a88a0428ec 1 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 3 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 4 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 5 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 6 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 7 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 9 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 10 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 11 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 12 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 14 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 15 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 16 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 17 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 18 ySgD0A+Wv9KWquaT6UjImd4o4ac acetaldehyde sensitivity ------- COMMENT: 80aa26a88a0428ec 19 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 21 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 24 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 25 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 29 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 42 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 44 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 45 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 46 ySgD0A+Wv9KWquaT6UjImd4o4ac (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 114 IjCVELuAS7NRejdbES93Q4kocs4 (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 116 IjCVELuAS7NRejdbES93Q4kocs4 (comment: CHECK acetaldehyde sensitivity) ------- COMMENT: 80aa26a88a0428ec 118 IjCVELuAS7NRejdbES93Q4kocs4 (comment: acetaldehyde absent) ------- COMMENT: 80b382d361649949 10 YtfB0XWWoDU8frX4mArpZtjKJJs (commentL P.P. Bgs4 and Ags1 abnormal localization in the septum membrane) ------- COMMENT: 80b382d361649949 11 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: 80b382d361649949 12 63lJrspdngi85iCcW7MnJFo9JsY figure 1 A,B ------- COMMENT: 80b382d361649949 13 0fEUXwIwv2/viJc02RU06bVPYRA fig 3C&D ------- COMMENT: 80b382d361649949 15 SS7ow250Z6k6xT2nGDsHqqhOzRo fig 5 (comment: SH3 domain of Cdc15 is required for the proper concentration of Pxl1 at the CAR) ------- COMMENT: 80b382d361649949 16 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: 80b382d361649949 17 BD97fzgWSjv8GWx6mpm0KJ8Qkik fig6 Coupling of the Actomyosin ring contraction and septation onsetRing sliding even after the onset of septum synthesis, causing a longitudinal deposition along the plasma membrane of linear β-glucan as detected by CW staining until septum ingression started ------- COMMENT: 80b382d361649949 18 aSH1UoHehaOSoogIzECd67T4pn4 figure 1 ------- COMMENT: 80b382d361649949 19 qbtDkxJJOQ6Xof/nxESUw63Tl88 Fig 2A and B fragmented with RLC strands ------- COMMENT: 80b382d361649949 21 e6VN1qquAtJEeY7Eq8Nb3sBCipg Fig 2D and 2E ------- COMMENT: 80b382d361649949 22 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 80b382d361649949 23 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: 80b382d361649949 26 +lGokglJTKvk0vvTDRIpieifdEo figure 4B ------- COMMENT: 80b382d361649949 27 9QQ7HthEXp/JWeCIYT7IquPfxAo fig 4C ------- COMMENT: 811ad3c8dcd877f3 1 uqAMsAh2LyhvpMQ3gzQYOGea4jk Deletion of the PPR motifs in Ppr10 moderately affected the growth of S. pombe cells on glucose-containing media but severely impaired the growth on glycerol-containing media (Fig. 1E), ------- COMMENT: 811ad3c8dcd877f3 2 vK7+ygbHGKk9d2g9sK8PeIX3zC0 The level of Ppr10ΔPPR-Myc is reduced compared with that of WT protein (Fig. 1B). We found that only the full- length Ppr10-Myc coimmunoprecipitated with Mpa1 from whole-cell extract, whereas the mutant Ppr10 did not (Fig. 1C). ------- COMMENT: 811ad3c8dcd877f3 3 zWjqgOXVRkkNYh8KEsrtpTLF5R8 deletion of PPR motifs in Ppr10 severely reduced the steady-state levels of Cob1 (subunit of the cyto- chrome bc 1 complex), the core subunits of cytochrome c oxidase (Cox1, Cox2, Cox3), and Atp6 (subunit of ATP syn- thase) (Fig. 1D). ------- COMMENT: 811ad3c8dcd877f3 4 zWjqgOXVRkkNYh8KEsrtpTLF5R8 deletion of PPR motifs in Ppr10 severely reduced the steady-state levels of Cob1 (subunit of the cyto- chrome bc 1 complex), the core subunits of cytochrome c oxidase (Cox1, Cox2, Cox3), and Atp6 (subunit of ATP syn- thase) (Fig. 1D). ------- COMMENT: 811ad3c8dcd877f3 5 zWjqgOXVRkkNYh8KEsrtpTLF5R8 deletion of PPR motifs in Ppr10 severely reduced the steady-state levels of Cob1 (subunit of the cyto- chrome bc 1 complex), the core subunits of cytochrome c oxidase (Cox1, Cox2, Cox3), and Atp6 (subunit of ATP syn- thase) (Fig. 1D). ------- COMMENT: 811ad3c8dcd877f3 6 zWjqgOXVRkkNYh8KEsrtpTLF5R8 deletion of PPR motifs in Ppr10 severely reduced the steady-state levels of Cob1 (subunit of the cyto- chrome bc 1 complex), the core subunits of cytochrome c oxidase (Cox1, Cox2, Cox3), and Atp6 (subunit of ATP syn- thase) (Fig. 1D). ------- COMMENT: 811ad3c8dcd877f3 7 zWjqgOXVRkkNYh8KEsrtpTLF5R8 deletion of PPR motifs in Ppr10 severely reduced the steady-state levels of Cob1 (subunit of the cyto- chrome bc 1 complex), the core subunits of cytochrome c oxidase (Cox1, Cox2, Cox3), and Atp6 (subunit of ATP syn- thase) (Fig. 1D). ------- COMMENT: 811ad3c8dcd877f3 8 HEGhnCfT8u8n80isATUKd/s+BTA We consistently found that disruption of ppr10, mpa1, or the PPR motifs of Ppr10 resulted in dissociation of Mti2 and Mti3 from the mt-SSU and reduced the association of Mti2 and Mti3 with mitor- ibosomes (Fig. 2, B–D). ------- COMMENT: 811ad3c8dcd877f3 9 HEGhnCfT8u8n80isATUKd/s+BTA We consistently found that disruption of ppr10, mpa1, or the PPR motifs of Ppr10 resulted in dissociation of Mti2 and Mti3 from the mt-SSU and reduced the association of Mti2 and Mti3 with mitor- ibosomes (Fig. 2, B–D). ------- COMMENT: 811ad3c8dcd877f3 10 HEGhnCfT8u8n80isATUKd/s+BTA We consistently found that disruption of ppr10, mpa1, or the PPR motifs of Ppr10 resulted in dissociation of Mti2 and Mti3 from the mt-SSU and reduced the association of Mti2 and Mti3 with mitor- ibosomes (Fig. 2, B–D). ------- COMMENT: 811ad3c8dcd877f3 11 HEGhnCfT8u8n80isATUKd/s+BTA We consistently found that disruption of ppr10, mpa1, or the PPR motifs of Ppr10 resulted in dissociation of Mti2 and Mti3 from the mt-SSU and reduced the association of Mti2 and Mti3 with mitor- ibosomes (Fig. 2, B–D). ------- COMMENT: 811ad3c8dcd877f3 12 HEGhnCfT8u8n80isATUKd/s+BTA We consistently found that disruption of ppr10, mpa1, or the PPR motifs of Ppr10 resulted in dissociation of Mti2 and Mti3 from the mt-SSU and reduced the association of Mti2 and Mti3 with mitor- ibosomes (Fig. 2, B–D). ------- COMMENT: 811ad3c8dcd877f3 13 HEGhnCfT8u8n80isATUKd/s+BTA We consistently found that disruption of ppr10, mpa1, or the PPR motifs of Ppr10 resulted in dissociation of Mti2 and Mti3 from the mt-SSU and reduced the association of Mti2 and Mti3 with mitor- ibosomes (Fig. 2, B–D). ------- COMMENT: 811ad3c8dcd877f3 14 fq+zj0yuOf1HiqO0iWj6MjGoaTI Disruption of ppr10, mpa1, or the PPR motifs of Ppr10 did not impair the assembly of mitoribosomes or their subunits (Fig. 2, A–D) and did not affect the steady-state levels of the protein subunits of the mt-SSU and mt-LSU (Fig. 2E). ------- COMMENT: 811ad3c8dcd877f3 15 fq+zj0yuOf1HiqO0iWj6MjGoaTI Disruption of ppr10, mpa1, or the PPR motifs of Ppr10 did not impair the assembly of mitoribosomes or their subunits (Fig. 2, A–D) and did not affect the steady-state levels of the protein subunits of the mt-SSU and mt-LSU (Fig. 2E). ------- COMMENT: 811ad3c8dcd877f3 16 fq+zj0yuOf1HiqO0iWj6MjGoaTI Disruption of ppr10, mpa1, or the PPR motifs of Ppr10 did not impair the assembly of mitoribosomes or their subunits (Fig. 2, A–D) and did not affect the steady-state levels of the protein subunits of the mt-SSU and mt-LSU (Fig. 2E). ------- COMMENT: 811ad3c8dcd877f3 17 fq+zj0yuOf1HiqO0iWj6MjGoaTI Disruption of ppr10, mpa1, or the PPR motifs of Ppr10 did not impair the assembly of mitoribosomes or their subunits (Fig. 2, A–D) and did not affect the steady-state levels of the protein subunits of the mt-SSU and mt-LSU (Fig. 2E). ------- COMMENT: 811ad3c8dcd877f3 18 fq+zj0yuOf1HiqO0iWj6MjGoaTI Disruption of ppr10, mpa1, or the PPR motifs of Ppr10 did not impair the assembly of mitoribosomes or their subunits (Fig. 2, A–D) and did not affect the steady-state levels of the protein subunits of the mt-SSU and mt-LSU (Fig. 2E). ------- COMMENT: 811ad3c8dcd877f3 19 fq+zj0yuOf1HiqO0iWj6MjGoaTI Disruption of ppr10, mpa1, or the PPR motifs of Ppr10 did not impair the assembly of mitoribosomes or their subunits (Fig. 2, A–D) and did not affect the steady-state levels of the protein subunits of the mt-SSU and mt-LSU (Fig. 2E). ------- COMMENT: 811ad3c8dcd877f3 20 fq+zj0yuOf1HiqO0iWj6MjGoaTI Disruption of ppr10, mpa1, or the PPR motifs of Ppr10 did not impair the assembly of mitoribosomes or their subunits (Fig. 2, A–D) and did not affect the steady-state levels of the protein subunits of the mt-SSU and mt-LSU (Fig. 2E). ------- COMMENT: 811ad3c8dcd877f3 21 fq+zj0yuOf1HiqO0iWj6MjGoaTI Disruption of ppr10, mpa1, or the PPR motifs of Ppr10 did not impair the assembly of mitoribosomes or their subunits (Fig. 2, A–D) and did not affect the steady-state levels of the protein subunits of the mt-SSU and mt-LSU (Fig. 2E). ------- COMMENT: 811ad3c8dcd877f3 22 fq+zj0yuOf1HiqO0iWj6MjGoaTI Disruption of ppr10, mpa1, or the PPR motifs of Ppr10 did not impair the assembly of mitoribosomes or their subunits (Fig. 2, A–D) and did not affect the steady-state levels of the protein subunits of the mt-SSU and mt-LSU (Fig. 2E). ------- COMMENT: 811ad3c8dcd877f3 23 fq+zj0yuOf1HiqO0iWj6MjGoaTI Disruption of ppr10, mpa1, or the PPR motifs of Ppr10 did not impair the assembly of mitoribosomes or their subunits (Fig. 2, A–D) and did not affect the steady-state levels of the protein subunits of the mt-SSU and mt-LSU (Fig. 2E). ------- COMMENT: 811ad3c8dcd877f3 24 fq+zj0yuOf1HiqO0iWj6MjGoaTI Disruption of ppr10, mpa1, or the PPR motifs of Ppr10 did not impair the assembly of mitoribosomes or their subunits (Fig. 2, A–D) and did not affect the steady-state levels of the protein subunits of the mt-SSU and mt-LSU (Fig. 2E). ------- COMMENT: 811ad3c8dcd877f3 25 p8+yrgunZXdU5CcUlVzwaGY/IHQ The association of cob1 and cox1 mRNAs with assembled mitor- ibosomes was reduced by ppr10 deletion, whereas their asso- ciation with the mt-SSU was increased (Fig. 3). ------- COMMENT: 811ad3c8dcd877f3 26 p8+yrgunZXdU5CcUlVzwaGY/IHQ The association of cob1 and cox1 mRNAs with assembled mitor- ibosomes was reduced by ppr10 deletion, whereas their asso- ciation with the mt-SSU was increased (Fig. 3). ------- COMMENT: 811ad3c8dcd877f3 38 c64R74to37N8OsqhOj3B23pGgI8 The PPR motifs of Ppr10 are not involved in the association of Ppr10 with a subset of mitoribosomal proteins ------- COMMENT: 811ad3c8dcd877f3 39 TxKz/uTGsX2cFBc2TtdfAdC5O1I Taken together, these data reveal that the PPR motifs in Ppr10 are critical for the Ppr10-Mpa1 interaction and that disruption of the PPR motifs in Ppr10 impairs mitochondrial protein synthesis and, consequently, respiratory growth of S. pombe cells. ------- COMMENT: 811ad3c8dcd877f3 41 EPXaHTG3lpVhF0+nGI8jOD2YHUU Disruption of ppr10, mpa1, or the PPR motifs of Ppr10 did not impair the assembly of mitoribosomes or their subunits (Fig. 2, A–D) a ------- COMMENT: 811ad3c8dcd877f3 42 oanSUSqoUI6O23TiAYTY6BnN7Hg We consistently found that disruption of ppr10, mpa1, or the PPR motifs of Ppr10 resulted in dissociation of Mti2 and Mti3 from the mt-SSU (Fig. 2, B–D). ------- COMMENT: 811ad3c8dcd877f3 43 oanSUSqoUI6O23TiAYTY6BnN7Hg We consistently found that disruption of ppr10, mpa1, or the PPR motifs of Ppr10 resulted in dissociation of Mti2 and Mti3 from the mt-SSU (Fig. 2, B–D). ------- COMMENT: 811ad3c8dcd877f3 44 oanSUSqoUI6O23TiAYTY6BnN7Hg We consistently found that disruption of ppr10, mpa1, or the PPR motifs of Ppr10 resulted in dissociation of Mti2 and Mti3 from the mt-SSU (Fig. 2, B–D). ------- COMMENT: 817e75f5ee938916 4 P9K/bHWScSDsN7CyxFwIH7xGW7c (comment: target genes: cut6, vht1, bio2) ------- COMMENT: 817e75f5ee938916 17 sI3SDi+HtiDlnnwcEmgSqT9V+uY (comment: CHECK with cut6-621 mutant allele) ------- COMMENT: 817e75f5ee938916 18 4ZmmyrNwePjR4EwQ9bNmmHIiza4 (comment: CHECK by cut6+ overexpression) ------- COMMENT: 819935350ed7d37f 113 zQxRNYpYchD2vELKxRXkPcWrQUE (comment: I think this is real i.e. downregulation of growth to allow differentiation) ------- COMMENT: 81cc9cd6e028de43 1 xiAQ3bHH4yDJfcTXWz8arqpAAxM Deletion of mrh5 did not reduce the protein level of Ppr4 (Fig. 1A) ------- COMMENT: 81cc9cd6e028de43 2 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 81cc9cd6e028de43 3 ItjOi6+FdyfbN78DEb6bjvafZn4 Additionally, the Mtf2 protein level was moderately reduced in Δmrh5 cells (Fig. 1A). ------- COMMENT: 81cc9cd6e028de43 4 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 81cc9cd6e028de43 6 wXBJHV9hA44rU2yQmxcxk0dUnZA Deletion of sls1 resulted in a drastic reduction in the protein level of Mtf2 (Fig. 1B). A ------- COMMENT: 81cc9cd6e028de43 7 WIMmKyBW8LZOXuTUBAr65g907Ok In contrast, the deletion of ppr4 and mtf2 did not or only moderately affect the protein levels of other Mrh5C subunits (Fig. 1, C and D). ------- COMMENT: 81cc9cd6e028de43 8 WIMmKyBW8LZOXuTUBAr65g907Ok In contrast, the deletion of ppr4 and mtf2 did not or only moderately affect the protein levels of other Mrh5C subunits (Fig. 1, C and D). ------- COMMENT: 81cc9cd6e028de43 9 WIMmKyBW8LZOXuTUBAr65g907Ok In contrast, the deletion of ppr4 and mtf2 did not or only moderately affect the protein levels of other Mrh5C subunits (Fig. 1, C and D). ------- COMMENT: 81cc9cd6e028de43 10 WIMmKyBW8LZOXuTUBAr65g907Ok In contrast, the deletion of ppr4 and mtf2 did not or only moderately affect the protein levels of other Mrh5C subunits (Fig. 1, C and D). ------- COMMENT: 81cc9cd6e028de43 11 WIMmKyBW8LZOXuTUBAr65g907Ok In contrast, the deletion of ppr4 and mtf2 did not or only moderately affect the protein levels of other Mrh5C subunits (Fig. 1, C and D). ------- COMMENT: 81cc9cd6e028de43 12 WIMmKyBW8LZOXuTUBAr65g907Ok In contrast, the deletion of ppr4 and mtf2 did not or only moderately affect the protein levels of other Mrh5C subunits (Fig. 1, C and D). ------- COMMENT: 81cc9cd6e028de43 13 AYWEjUdRLzLXYdDLzHTtrPLbnCk Co- immunoprecipitation experiments with anti-FLAG beads showed that deletion of mtf2 abolished the interaction of Sls1- FLAG with Mrh5-Myc and Ppr4-CBP (Fig. 2A). ------- COMMENT: 81cc9cd6e028de43 14 AYWEjUdRLzLXYdDLzHTtrPLbnCk Co- immunoprecipitation experiments with anti-FLAG beads showed that deletion of mtf2 abolished the interaction of Sls1- FLAG with Mrh5-Myc and Ppr4-CBP (Fig. 2A). ------- COMMENT: 81cc9cd6e028de43 15 AYWEjUdRLzLXYdDLzHTtrPLbnCk Co- immunoprecipitation experiments with anti-FLAG beads showed that deletion of mtf2 abolished the interaction of Sls1- FLAG with Mrh5-Myc and Ppr4-CBP (Fig. 2A). ------- COMMENT: 81cc9cd6e028de43 16 W20DUqBkhE3iivzBJD949Niswng Co- immunoprecipitation experiments using anti-Myc beads revealed that loss of sls1 abolished the association of Mrh5-Myc with Prp4-CBP and Mtf2-HA (Fig. 2C). ------- COMMENT: 81cc9cd6e028de43 17 W20DUqBkhE3iivzBJD949Niswng Co- immunoprecipitation experiments using anti-Myc beads revealed that loss of sls1 abolished the association of Mrh5-Myc with Prp4-CBP and Mtf2-HA (Fig. 2C). ------- COMMENT: 81cc9cd6e028de43 18 UT9kKEqVrwvgcbStxiXLtf7KdMo Deletion of mrh5 did not impair the interaction among Prp4-CBP, Sls1-FLAG, and Mtf2-HA (Fig. 2D). ------- COMMENT: 81cc9cd6e028de43 19 UT9kKEqVrwvgcbStxiXLtf7KdMo Deletion of mrh5 did not impair the interaction among Prp4-CBP, Sls1-FLAG, and Mtf2-HA (Fig. 2D). ------- COMMENT: 81cc9cd6e028de43 20 0XwEatrpzzK7QOTg0QioUxobiig Similarly, deletion of ppr4 did not impair the inter- action among Mrh5-Myc, Sls1-FLAG, and Mtf2-HA (Fig. 2E). ------- COMMENT: 81cc9cd6e028de43 21 0XwEatrpzzK7QOTg0QioUxobiig Similarly, deletion of ppr4 did not impair the inter- action among Mrh5-Myc, Sls1-FLAG, and Mtf2-HA (Fig. 2E). ------- COMMENT: 81cc9cd6e028de43 22 mkGqcsmcemYfNFZc7NptCPom5yI Mrh5C subunits no longer associate with each other. Mrh5 co- immunoprecipitated with the mtSSU as expected (Fig. 3A). Deletion of mtf2 dramatically reduced this association, suggesting that the whole complex is involved in mtSSU binding (Fig. 3A). ------- COMMENT: 81cc9cd6e028de43 24 OvDGGDowFPpEaPrRMB9qiJc4JyE Deletion of mtf2 or other subunits of Mrh5C dramatically reduced the levels of mtSSU proteins but not the large subunit of the mitoribosome (mtLSU) proteins tested (Figure 3, B and C), ------- COMMENT: 81cc9cd6e028de43 25 OvDGGDowFPpEaPrRMB9qiJc4JyE Deletion of mtf2 or other subunits of Mrh5C dramatically reduced the levels of mtSSU proteins but not the large subunit of the mitoribosome (mtLSU) proteins tested (Figure 3, B and C), ------- COMMENT: 81cc9cd6e028de43 26 OvDGGDowFPpEaPrRMB9qiJc4JyE Deletion of mtf2 or other subunits of Mrh5C dramatically reduced the levels of mtSSU proteins but not the large subunit of the mitoribosome (mtLSU) proteins tested (Figure 3, B and C), ------- COMMENT: 81cc9cd6e028de43 27 OvDGGDowFPpEaPrRMB9qiJc4JyE Deletion of mtf2 or other subunits of Mrh5C dramatically reduced the levels of mtSSU proteins but not the large subunit of the mitoribosome (mtLSU) proteins tested (Figure 3, B and C), ------- COMMENT: 81cc9cd6e028de43 28 OvDGGDowFPpEaPrRMB9qiJc4JyE Deletion of mtf2 or other subunits of Mrh5C dramatically reduced the levels of mtSSU proteins but not the large subunit of the mitoribosome (mtLSU) proteins tested (Figure 3, B and C), ------- COMMENT: 81cc9cd6e028de43 29 MMJVDJSb6duz1MFP/BzJuaMchnU In contrast, Mrh5C subunits no longer co-sedimented with the mtSSU in Δmtf2 cells (Fig. 3D) ------- COMMENT: 81cc9cd6e028de43 30 d0DDk/nrHeoyiYgQMj1V/l9l8yI In contrast, Mrh5C subunits no longer co-sedimented with the mtSSU in Δmtf2 cells (Fig. 3D) ------- COMMENT: 81cc9cd6e028de43 31 np8RUO/21+QEwtP15SzgxbvQFxo In contrast, Mrh5C subunits no longer co-sedimented with the mtSSU in Δmtf2 cells (Fig. 3D) ------- COMMENT: 81cc9cd6e028de43 32 tP4yCiHiQA8Nw7gnLMHEGgdEI40 In control cells (Δpnu1[Δi]), the cox1 and cob1 mRNAs appeared in two peaks, a major peak (peak A) comigrating with the mtSSU and a second peak (peak B) comigrating with the mitoribosome (Fig. 4). In Δmtf2 cells, peak A was up-shifted to the lower-density regions and did not comigrate with the mtSSU, and peak B decreased dramatically (Fig. 4). ------- COMMENT: 81cc9cd6e028de43 33 tP4yCiHiQA8Nw7gnLMHEGgdEI40 In control cells (Δpnu1[Δi]), the cox1 and cob1 mRNAs appeared in two peaks, a major peak (peak A) comigrating with the mtSSU and a second peak (peak B) comigrating with the mitoribosome (Fig. 4). In Δmtf2 cells, peak A was up-shifted to the lower-density regions and did not comigrate with the mtSSU, and peak B decreased dramatically (Fig. 4). ------- COMMENT: 81cc9cd6e028de43 44 svEYpZGkQsc4VqhPBbeMiXTl79I A single Asp261 to Ala or Glu262 to Ala mutation gave rise to a substantially reduced level of cox1 mRNA but little or no reduction of the levels of other mt- mRNAs and mt-rRNAs (Fig. 5A). ------- COMMENT: 81cc9cd6e028de43 45 ZeZc6ZO0mncqsXihsItuVnyxHNw (comment: [35S]-methionine/cysteine labeling) ------- COMMENT: 81cc9cd6e028de43 46 JoWc4X3X4Wz2UUkQnvmUsDIe7Uo These mutations also completely abolished Cox1 synthesis and decreased the syn- thesis of other mtDNA-encoded proteins (Fig. 5, B and C). ------- COMMENT: 81cc9cd6e028de43 47 Zmq5/gF42BO5F8fKq1ZYhexTba4 (comment: CHECK ABOLISHED ********) These mutations also completely abolished Cox1 synthesis and decreased the syn- thesis of other mtDNA-encoded proteins (Fig. 5, B and C). ------- COMMENT: 81cc9cd6e028de43 48 JoWc4X3X4Wz2UUkQnvmUsDIe7Uo These mutations also completely abolished Cox1 synthesis and decreased the syn- thesis of other mtDNA-encoded proteins (Fig. 5, B and C). ------- COMMENT: 81cc9cd6e028de43 49 JoWc4X3X4Wz2UUkQnvmUsDIe7Uo These mutations also completely abolished Cox1 synthesis and decreased the syn- thesis of other mtDNA-encoded proteins (Fig. 5, B and C). ------- COMMENT: 81cc9cd6e028de43 50 JoWc4X3X4Wz2UUkQnvmUsDIe7Uo These mutations also completely abolished Cox1 synthesis and decreased the syn- thesis of other mtDNA-encoded proteins (Fig. 5, B and C). ------- COMMENT: 81cc9cd6e028de43 52 ZeZc6ZO0mncqsXihsItuVnyxHNw (comment: [35S]-methionine/cysteine labeling) ------- COMMENT: 81cc9cd6e028de43 53 Lv3mY2VxrxtU7+lrIcCWOox9KbQ These mutations also completely abolished Cox1 synthesis and decreased the syn- thesis of other mtDNA-encoded proteins (Fig. 5, B and C) ------- COMMENT: 81cc9cd6e028de43 54 oOWd2St+ed3CI8vxt/6ahZ8BG4M These mutations also completely abolished Cox1 synthesis and decreased the syn- thesis of other mtDNA-encoded proteins (Fig. 5, B and C) ------- COMMENT: 81cc9cd6e028de43 55 Lv3mY2VxrxtU7+lrIcCWOox9KbQ These mutations also completely abolished Cox1 synthesis and decreased the syn- thesis of other mtDNA-encoded proteins (Fig. 5, B and C) ------- COMMENT: 81cc9cd6e028de43 56 Lv3mY2VxrxtU7+lrIcCWOox9KbQ These mutations also completely abolished Cox1 synthesis and decreased the syn- thesis of other mtDNA-encoded proteins (Fig. 5, B and C) ------- COMMENT: 81cc9cd6e028de43 57 Lv3mY2VxrxtU7+lrIcCWOox9KbQ These mutations also completely abolished Cox1 synthesis and decreased the syn- thesis of other mtDNA-encoded proteins (Fig. 5, B and C) ------- COMMENT: 81cc9cd6e028de43 58 ZeZc6ZO0mncqsXihsItuVnyxHNw (comment: [35S]-methionine/cysteine labeling) ------- COMMENT: 81cc9cd6e028de43 65 ivChirpshXY/5LABvatlVKGj9FI It is likely that the abolishment of Cox1 synthesis results in the downregulation of OXPHOS complexes. Consistent with these results, cells harboring mrh5D261A-Myc or mrh5E262A-Myc could not grow on glycerol-containing medium, suggesting that they are respiratory-deficient (Fig. S5). ------- COMMENT: 81cc9cd6e028de43 66 ivChirpshXY/5LABvatlVKGj9FI It is likely that the abolishment of Cox1 synthesis results in the downregulation of OXPHOS complexes. Consistent with these results, cells harboring mrh5D261A-Myc or mrh5E262A-Myc could not grow on glycerol-containing medium, suggesting that they are respiratory-deficient (Fig. S5). ------- COMMENT: 81cc9cd6e028de43 67 tFaaimzlgqT+Xli+pkWwa8dwxao Mutations of the DEAD-box in Mrh5-Myc resulted in the dissociation of the Mrh5 mutants with the mtSSU (Figs. 7 and S6). ------- COMMENT: 81cc9cd6e028de43 68 tFaaimzlgqT+Xli+pkWwa8dwxao Mutations of the DEAD-box in Mrh5-Myc resulted in the dissociation of the Mrh5 mutants with the mtSSU (Figs. 7 and S6). ------- COMMENT: 81cc9cd6e028de43 69 RVxHhb+a8sqjOoxMbyCkr5Ik1Og Immunoblot analysis of factions revealed that mutation of the DEAD-box of Mrh5 abol- ished the association of the cox1 mRNA with the mtSSU (Fig. 8). ------- COMMENT: 81cc9cd6e028de43 70 RVxHhb+a8sqjOoxMbyCkr5Ik1Og Immunoblot analysis of factions revealed that mutation of the DEAD-box of Mrh5 abol- ished the association of the cox1 mRNA with the mtSSU (Fig. 8). ------- COMMENT: 81cc9cd6e028de43 71 RVxHhb+a8sqjOoxMbyCkr5Ik1Og Immunoblot analysis of factions revealed that mutation of the DEAD-box of Mrh5 abol- ished the association of the cox1 mRNA with the mtSSU (Fig. 8). ------- COMMENT: 81cc9cd6e028de43 72 RVxHhb+a8sqjOoxMbyCkr5Ik1Og Immunoblot analysis of factions revealed that mutation of the DEAD-box of Mrh5 abol- ished the association of the cox1 mRNA with the mtSSU (Fig. 8). ------- COMMENT: 81cc9cd6e028de43 73 RVxHhb+a8sqjOoxMbyCkr5Ik1Og Immunoblot analysis of factions revealed that mutation of the DEAD-box of Mrh5 abol- ished the association of the cox1 mRNA with the mtSSU (Fig. 8). ------- COMMENT: 81cc9cd6e028de43 74 RVxHhb+a8sqjOoxMbyCkr5Ik1Og Immunoblot analysis of factions revealed that mutation of the DEAD-box of Mrh5 abol- ished the association of the cox1 mRNA with the mtSSU (Fig. 8). ------- COMMENT: 81cc9cd6e028de43 75 RVxHhb+a8sqjOoxMbyCkr5Ik1Og Immunoblot analysis of factions revealed that mutation of the DEAD-box of Mrh5 abol- ished the association of the cox1 mRNA with the mtSSU (Fig. 8). ------- COMMENT: 81cc9cd6e028de43 76 RVxHhb+a8sqjOoxMbyCkr5Ik1Og Immunoblot analysis of factions revealed that mutation of the DEAD-box of Mrh5 abol- ished the association of the cox1 mRNA with the mtSSU (Fig. 8). ------- COMMENT: 81cc9cd6e028de43 77 2+NMtJTk0/So3JDyobj4/7f4ssw Mrh5C subunits no longer associate with each other. Mrh5 co- immunoprecipitated with the mtSSU as expected (Fig. 3A). Deletion of mtf2 dramatically reduced this association, suggesting that the whole complex is involved in mtSSU binding (Fig. 3A). |Mrh5C was predominantly associated with the mtSSU in WT cells (Fig. 3D) ------- COMMENT: 81cdad3b15bdd1e4 16 IRbwbmGruEtNEtDmmDbtVe0lyLk (comment: CHECK high penetrance = large fraction of cells) ------- COMMENT: 81e91219a1043368 2 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 81e91219a1043368 4 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 81e91219a1043368 13 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 81e91219a1043368 14 a1dM/ys/9G8EDQ9Dfhxcm3CjECg Figure 3 A ------- COMMENT: 81e91219a1043368 15 a1dM/ys/9G8EDQ9Dfhxcm3CjECg Figure 3 A ------- COMMENT: 81e91219a1043368 16 WJFvCpy9xDYIZUsz5hsJUgTFgi0 (comment: CHECK ABOLISHED Figure 3B) ------- COMMENT: 82e092beb9dd503e 1 a8RbYPxyb4TryQgXmYU92FlHfQM Fig. 1A and B ------- COMMENT: 82e092beb9dd503e 2 bQ398RDH40pzL6Cxr4naZ2FYtrU Fig. S1E and F ------- COMMENT: 82e092beb9dd503e 3 bQ398RDH40pzL6Cxr4naZ2FYtrU Fig. S1E and F ------- COMMENT: 82e092beb9dd503e 4 IpCb2zqaElpWeG34wcUjxYWXCVA Our studies have shed light on novel functions of the MOR in non-centrosomal MTOCs and cytoplasmic microtubule organization. ------- COMMENT: 82e092beb9dd503e 5 bQ398RDH40pzL6Cxr4naZ2FYtrU Fig. S1E and F ------- COMMENT: 82e092beb9dd503e 6 IpCb2zqaElpWeG34wcUjxYWXCVA Our studies have shed light on novel functions of the MOR in non-centrosomal MTOCs and cytoplasmic microtubule organization. ------- COMMENT: 82e092beb9dd503e 7 IpCb2zqaElpWeG34wcUjxYWXCVA Our studies have shed light on novel functions of the MOR in non-centrosomal MTOCs and cytoplasmic microtubule organization. ------- COMMENT: 82e092beb9dd503e 8 IpCb2zqaElpWeG34wcUjxYWXCVA Our studies have shed light on novel functions of the MOR in non-centrosomal MTOCs and cytoplasmic microtubule organization. ------- COMMENT: 82e092beb9dd503e 9 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 82e092beb9dd503e 10 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 82e092beb9dd503e 11 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 82e092beb9dd503e 12 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: 82e092beb9dd503e 13 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 82e092beb9dd503e 14 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 82e092beb9dd503e 15 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 82e092beb9dd503e 16 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 82e092beb9dd503e 17 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 82e092beb9dd503e 18 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 82e092beb9dd503e 19 +3YOxH0b1V3IJTmnRZsMG8IweL0 Fig. 2A, B and C ------- COMMENT: 82e092beb9dd503e 20 +3YOxH0b1V3IJTmnRZsMG8IweL0 Fig. 2A, B and C ------- COMMENT: 82e092beb9dd503e 21 +3YOxH0b1V3IJTmnRZsMG8IweL0 Fig. 2A, B and C ------- COMMENT: 82e092beb9dd503e 22 TF8ZRTMmVnD1zrHyMyCpkiqZEm0 Fig. 2D, E and F ------- COMMENT: 82e092beb9dd503e 23 TF8ZRTMmVnD1zrHyMyCpkiqZEm0 Fig. 2D, E and F ------- COMMENT: 82e092beb9dd503e 24 TF8ZRTMmVnD1zrHyMyCpkiqZEm0 Fig. 2D, E and F ------- COMMENT: 82e092beb9dd503e 25 TF8ZRTMmVnD1zrHyMyCpkiqZEm0 Fig. 2D, E and F ------- COMMENT: 82e092beb9dd503e 26 /GLZVXrrquv9q+970jbmzv8iC2Q Fig. 3A and C ------- COMMENT: 82e092beb9dd503e 27 /GLZVXrrquv9q+970jbmzv8iC2Q Fig. 3A and C ------- COMMENT: 82e092beb9dd503e 28 /GLZVXrrquv9q+970jbmzv8iC2Q Fig. 3A and C ------- COMMENT: 82e092beb9dd503e 29 Mij9SBEPt4GIt/vtUBP2T7kXS+o Fig. 3B and C ------- COMMENT: 82e092beb9dd503e 30 Mij9SBEPt4GIt/vtUBP2T7kXS+o Fig. 3B and C ------- COMMENT: 82e092beb9dd503e 31 Mij9SBEPt4GIt/vtUBP2T7kXS+o Fig. 3B and C ------- COMMENT: 82e092beb9dd503e 32 Xo246k9raXfJx7NCgHAfZSFYv0U Fig. 3D, E and F ------- COMMENT: 82e092beb9dd503e 33 Xo246k9raXfJx7NCgHAfZSFYv0U Fig. 3D, E and F ------- COMMENT: 82e092beb9dd503e 34 Xo246k9raXfJx7NCgHAfZSFYv0U Fig. 3D, E and F ------- COMMENT: 82e092beb9dd503e 35 Xo246k9raXfJx7NCgHAfZSFYv0U Fig. 3D, E and F ------- COMMENT: 82e092beb9dd503e 36 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 82e092beb9dd503e 37 bQ398RDH40pzL6Cxr4naZ2FYtrU Fig. S1E and F ------- COMMENT: 82e092beb9dd503e 38 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 82e092beb9dd503e 39 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 82e092beb9dd503e 40 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 82e092beb9dd503e 41 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 82e092beb9dd503e 42 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 82e092beb9dd503e 43 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: 83235993b938b667 8 NYZc9M9d7w29y/DwgppccuRwrTk skb1∆ and srr1∆ additively reduce the rate of gross chromosomal rearrangements in rad51 deletion background. ------- COMMENT: 83235993b938b667 9 vATwJOxfLYO2dgZ3DcgZ49LngNQ figure 3a ------- COMMENT: 83235993b938b667 10 vATwJOxfLYO2dgZ3DcgZ49LngNQ figure 3a ------- COMMENT: 83235993b938b667 11 vATwJOxfLYO2dgZ3DcgZ49LngNQ figure 3a ------- COMMENT: 83235993b938b667 12 07O03dVI4O58QvW9Qj+2YAPeJjY To our surprise, not only srr1Δ but also skb1Δ reduced GCR rates in the rad51Δ background, demonstrating that both Srr1 and Skb1 cause GCRs. ------- COMMENT: 83235993b938b667 13 zhelB5q/zocZeE+fMheWi0TM/Tw Normal Chk1 phosphorylation and cell cycle arrest ------- COMMENT: 83235993b938b667 15 05l6aFwNyWD71t3YThUzvlN6MyY srr1-W157R and rad52-R45K or pcn1-K107R additively reduce gross chromosomal rearrangement. srr1-W157R and pcn1-K107R also addi- tively reduced GCR rates in rad51Δ cells (Fig. 4a). ------- COMMENT: 83235993b938b667 16 vATwJOxfLYO2dgZ3DcgZ49LngNQ figure 3a ------- COMMENT: 83235993b938b667 17 vATwJOxfLYO2dgZ3DcgZ49LngNQ figure 3a ------- COMMENT: 83235993b938b667 18 vATwJOxfLYO2dgZ3DcgZ49LngNQ figure 3a ------- COMMENT: 83235993b938b667 19 KaKxCaJ39QxJDwR8MuYpwyIFy0Q Both srr1-D111A,P112A and srr1- H148A mutations reduced GCR rates (Fig. 5d). ------- COMMENT: 83235993b938b667 20 +8LvJaDE3WzlBy29MD9KgIbBggc figure 5e ------- COMMENT: 83235993b938b667 21 +8LvJaDE3WzlBy29MD9KgIbBggc figure 5e ------- COMMENT: 83235993b938b667 22 +8LvJaDE3WzlBy29MD9KgIbBggc figure 5e ------- COMMENT: 83235993b938b667 24 +8LvJaDE3WzlBy29MD9KgIbBggc figure 5e ------- COMMENT: 83235993b938b667 25 +8LvJaDE3WzlBy29MD9KgIbBggc figure 5e ------- COMMENT: 83235993b938b667 26 +8LvJaDE3WzlBy29MD9KgIbBggc figure 5e ------- COMMENT: 83235993b938b667 27 t/z/T8oZDP82nX3Ao9ywhXe7kzI Fig 1e Genome sequencing of one of them identified the srr1/ ber1-W157R and skb1-A377V mutations in their SRR1-like and arginine methyltransferase (RMTase) domains, respectively (Fig. 1b). ------- COMMENT: 83235993b938b667 30 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 83235993b938b667 31 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 83235993b938b667 32 hfGxDCFZtcOTsZfoc2W1x+pzVT8 (Fig. 3e) ------- COMMENT: 83235993b938b667 33 07O03dVI4O58QvW9Qj+2YAPeJjY To our surprise, not only srr1Δ but also skb1Δ reduced GCR rates in the rad51Δ background, demonstrating that both Srr1 and Skb1 cause GCRs. ------- COMMENT: 83235993b938b667 34 vATwJOxfLYO2dgZ3DcgZ49LngNQ figure 3a ------- COMMENT: 83235993b938b667 35 vATwJOxfLYO2dgZ3DcgZ49LngNQ figure 3a ------- COMMENT: 83235993b938b667 36 vATwJOxfLYO2dgZ3DcgZ49LngNQ figure 3a ------- COMMENT: 83235993b938b667 38 hfGxDCFZtcOTsZfoc2W1x+pzVT8 (Fig. 3e) ------- COMMENT: 83235993b938b667 41 zhelB5q/zocZeE+fMheWi0TM/Tw Normal Chk1 phosphorylation and cell cycle arrest ------- COMMENT: 83235993b938b667 42 gmgIobVSqeufGm4EretcPB938Fk We crossed srr1Δ and rad52Δ haploid strains and dissected the tetrads but failed to obtain srr1Δ rad52Δ progenies (Fig. 4b), ------- COMMENT: 83235993b938b667 43 RXilW2j2PHjsh5fTz5o+2hRGf60 In the wild-type background, srr1Δ but not skb1Δ slightly reduced GCR rates, showing that Srr1 is required for GCRs even in the presence of Rad51 ------- COMMENT: 83235993b938b667 47 ANQXuVQhHv7QIny6v5grm1xhAjc Unlike chk1Δ, in the srr1Δ strain, the septation index declined to the wild-type level by 6 h after MMS addition, suggesting that Srr1 is dispensable for cell cycle arrest. ------- COMMENT: 83235993b938b667 49 FYsCoJnWzT0LTR62PEoZ4AwPKQA (Fig. 3e) We found that srr1Δ and srr1-W157R increased the rate of chromosome loss. (In WT and rad51 backgrounds) ------- COMMENT: 83235993b938b667 53 Q/QOhqb7PLRjkrMLw6juyc3GNOs The rad52-R45K, rad52Δ, and srr1Δ mutations eliminate ~90% of isochromosomes in rad51Δ cells (Fig. 2c and ref. 32), indicating that both Rad52 and Srr1 are essential for the major pathway of isochromosome formation. ------- COMMENT: 83235993b938b667 55 MLY1JMzoCQBzMV/7y7yw05TfUIU Like srr1Δ cells, srr1-W157R, srr1-D111A,P112A, and srr1-H184A cells produced small colonies (Supplementary Fig. 2b), consistent with the role of the SRR1-like domain even in the absence of exogenous DNA damage. ------- COMMENT: 83235993b938b667 56 MLY1JMzoCQBzMV/7y7yw05TfUIU Like srr1Δ cells, srr1-W157R, srr1-D111A,P112A, and srr1-H184A cells produced small colonies (Supplementary Fig. 2b), consistent with the role of the SRR1-like domain even in the absence of exogenous DNA damage. ------- COMMENT: 83235993b938b667 57 MLY1JMzoCQBzMV/7y7yw05TfUIU Like srr1Δ cells, srr1-W157R, srr1-D111A,P112A, and srr1-H184A cells produced small colonies (Supplementary Fig. 2b), consistent with the role of the SRR1-like domain even in the absence of exogenous DNA damage. ------- COMMENT: 83235993b938b667 58 MLY1JMzoCQBzMV/7y7yw05TfUIU Like srr1Δ cells, srr1-W157R, srr1-D111A,P112A, and srr1-H184A cells produced small colonies (Supplementary Fig. 2b), consistent with the role of the SRR1-like domain even in the absence of exogenous DNA damage. ------- COMMENT: 83235993b938b667 59 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 83235993b938b667 60 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 8355ff75101b4d60 18 BOQK3tLZzmcurhaAcvbrk6+SuTg (comment: CHECK this should be decreased thickness at old end during veg growth) ------- COMMENT: 83887018316db0aa 7 cp//HcWaZsHHHd4bpeys+nj9pTI Thus we found that wildtype Cat1-GFP cells growing in nitrogen-starvation conditions is localised to the plasma membrane, particularly at the growing cell ends, as previously reported [12,26]. ------- COMMENT: 839bb5efe129c7dc 1 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 839bb5efe129c7dc 2 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 839bb5efe129c7dc 3 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 839bb5efe129c7dc 4 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 839bb5efe129c7dc 5 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 839bb5efe129c7dc 6 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 839bb5efe129c7dc 7 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 839bb5efe129c7dc 8 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 839bb5efe129c7dc 9 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 839bb5efe129c7dc 10 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 839bb5efe129c7dc 11 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 839bb5efe129c7dc 12 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: 839bb5efe129c7dc 13 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 839bb5efe129c7dc 14 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 839bb5efe129c7dc 15 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 839bb5efe129c7dc 16 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 839bb5efe129c7dc 17 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 839bb5efe129c7dc 18 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 839bb5efe129c7dc 19 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 839bb5efe129c7dc 20 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 839bb5efe129c7dc 21 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 839bb5efe129c7dc 22 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 839bb5efe129c7dc 23 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 839bb5efe129c7dc 24 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: 839bb5efe129c7dc 25 caVjwT7Wwv+enQt3oStONIEXJUw Fig 3; (comment: same as either single mutant) ------- COMMENT: 839bb5efe129c7dc 26 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 839bb5efe129c7dc 27 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 839bb5efe129c7dc 28 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 839bb5efe129c7dc 29 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 839bb5efe129c7dc 30 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 839bb5efe129c7dc 31 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 839bb5efe129c7dc 32 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 839bb5efe129c7dc 33 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 839bb5efe129c7dc 34 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 839bb5efe129c7dc 35 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 839bb5efe129c7dc 36 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: 839bb5efe129c7dc 37 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: 839bb5efe129c7dc 38 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 839bb5efe129c7dc 39 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: 839bb5efe129c7dc 40 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: 839bb5efe129c7dc 41 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: 839bb5efe129c7dc 42 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: 839bb5efe129c7dc 43 dh18T+jjPYHGM8Br/n0yYr02rS8 (comment: CHECK interaction occurs upon HU or ionizing radiation exposure) ------- COMMENT: 839bb5efe129c7dc 54 E4kr8YSmWMjiNrkSSColzhEV01k (comment: basal phosphorylation on T412 & S423) ------- COMMENT: 83bb6f203d9fe65a 3 +UDYpQw89iaZ9s+L2cTgm3JD/mI Fig. 1 D–F ------- COMMENT: 83bb6f203d9fe65a 4 5CAXHLOEmWSiXGYEqqhVcBX0KN4 (comment: severe leaking) Fig. 3B, arrow; Fig. 3 C and D, quantifi- cation, figure 4 B ------- COMMENT: 83bb6f203d9fe65a 5 sznUYputP2Z+UqTKhgPd6hY+DhU Fig. 2B ------- COMMENT: 83bb6f203d9fe65a 7 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: 83bb6f203d9fe65a 8 er3xQLhNtwVU9IwkiX9si0hcWpQ Fig. 1B, supp table S1 ------- COMMENT: 83bb6f203d9fe65a 9 er3xQLhNtwVU9IwkiX9si0hcWpQ Fig. 1B, supp table S1 ------- COMMENT: 83bb6f203d9fe65a 10 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 83bb6f203d9fe65a 11 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: 83bb6f203d9fe65a 12 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: 83bb6f203d9fe65a 13 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: 83bb6f203d9fe65a 14 RWWcNH5OZW8RJH+3j3XXrNOEkw4 Fig S1AB ------- COMMENT: 83bb6f203d9fe65a 15 JSxsd7LXSwDvAx25miZXphBM7a8 (comment: CHECK rescue) ------- COMMENT: 83bb6f203d9fe65a 16 jZpr1TT1LES/M5S+prS1JSQPW2o Fig S1C ------- COMMENT: 83bb6f203d9fe65a 17 jZpr1TT1LES/M5S+prS1JSQPW2o Fig S1C ------- COMMENT: 83bb6f203d9fe65a 18 4YyiSZPBjurnsaDC7VCP2f7edUA 4B, arrowheads, and Fig. 5B ------- COMMENT: 83bb6f203d9fe65a 21 hhyqpg0V3etWHhw/nx1fuL2AL0c (comment: partial leaking) Fig. 3B, arrow; Fig. 3 C and D, quantifi- cation ------- COMMENT: 83bb6f203d9fe65a 22 hhyqpg0V3etWHhw/nx1fuL2AL0c (comment: partial leaking) Fig. 3B, arrow; Fig. 3 C and D, quantifi- cation ------- COMMENT: 83bb6f203d9fe65a 23 hhyqpg0V3etWHhw/nx1fuL2AL0c (comment: partial leaking) Fig. 3B, arrow; Fig. 3 C and D, quantifi- cation ------- COMMENT: 83bb6f203d9fe65a 24 O2pwFVGiLIAzJer6Qs3SvPi6tBI cells with abnormal NE morphology at the beginning of the exper- iment, by contrast, lost nuclear GFP completely over the time course (Fig. S3B). Thus, cytoplasmic GFP resulted from loss of nuclear integrity rather than from defects in nuclear import. ------- COMMENT: 83bb6f203d9fe65a 25 +5kQUUVzzO6le7+k69wOQ2xe5KY Fig. 4B, asterisk ------- COMMENT: 83bb6f203d9fe65a 26 HxAPxPGvdi0G3vMVrDlpm6Pr8GQ and the NPCs that were present were localized to re- gions that were largely free of karmellae and tubulo-vesicular structures (Fig. 4D). ------- COMMENT: 83bb6f203d9fe65a 27 mvz/JIlhpiFCz0gyvQ1lOIGaBKQ Remarkably, double-mutant cells displayed WT-like nuclei, although a few examples of probable nuclear fenestrations were observed in lem2Δ single-mutant cells (Fig. 5G). ------- COMMENT: 83bb6f203d9fe65a 28 mvz/JIlhpiFCz0gyvQ1lOIGaBKQ Remarkably, double-mutant cells displayed WT-like nuclei, although a few examples of probable nuclear fenestrations were observed in lem2Δ single-mutant cells (Fig. 5G). ------- COMMENT: 83c8a0868d64f0a1 59 JvqKiF7syn97ZfVC9qlsi0ZVvfw fig9 ------- COMMENT: 83c8a0868d64f0a1 60 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 83c8a0868d64f0a1 61 uP2b450kx3y/u6z3D7bDUa+eEuM fig8 ------- COMMENT: 83c8a0868d64f0a1 62 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 83cfcf15c86bcfbb 1 Lpura9CaeEIcX1uDWeWY5uXReEM This revealed that Sty1- Tpx1- and Sty1-Tpx1C48S-expressing cells both divided at a significantly smaller size than wild-type cells (Figure 1F). ------- COMMENT: 83cfcf15c86bcfbb 2 /8puQaCN0MS0+DG2nr3YVgqi4SI Figure 1 Strikingly, Sty1 phosphorylation was increased in cells ex- pressing either Sty1-Tpx1 or Sty1-Tpx1C48S (Figure 1D) ------- COMMENT: 83cfcf15c86bcfbb 3 mH6DTCTL7l+JAPPtCyyIg7QX5tY Indeed, consistent with the requirement of Wis1-dependent phosphorylation of Sty1 for timely entry into mitosis, cells expressing Wis1M395G or Wis1M395A were elongated (Figure S6B). ------- COMMENT: 83cfcf15c86bcfbb 4 mH6DTCTL7l+JAPPtCyyIg7QX5tY Indeed, consistent with the requirement of Wis1-dependent phosphorylation of Sty1 for timely entry into mitosis, cells expressing Wis1M395G or Wis1M395A were elongated (Figure S6B). ------- COMMENT: 83cfcf15c86bcfbb 5 NLVNEeNxzNI12HTqBsvKWSE3gX0 Our analysis revealed that Sty15CS- mutant-expressing cells were longer at the point of division than wild-type cells, strongly suggesting that Sty15CS’s pro- mitotic function was compromised (Figures S1D and S1E). ------- COMMENT: 83cfcf15c86bcfbb 6 JQmgOwyFMZ/L70UyJ0v36HYaqIM Moreover, when we crossed strains expressing Sty1-Tpx1 fusion and Dpyp1 mutant alleles, we observed a very strong synthetic lethal interaction, with 95% of spores with a sty1-tpx1 Dpyp1 genotype failing to give rise to a colony (Figure 2I and not shown). This strongly suggests that sty1- tpx1 and Dpyp1 alleles act independently to increase Sty1 phosphorylation, thus increasing it to lethal levels when co-ex- pressed. Indeed, analysis of colonies bearing markers of both al- leles revealed that surviving cells had completely eliminated Sty1-Tpx1 expression and activity (Figures 2J and 2K and not shown). ------- COMMENT: 83cfcf15c86bcfbb 7 me9lDthI5xf63yegfNIy2IuHuFI Figure 3 Sty1-Tpx1 fusion proteins, Wis1 was hyperphosphorylated to less electrophoretically mo- bile forms even in the absence of stress (Figures 3A and 3D). ------- COMMENT: 83cfcf15c86bcfbb 9 KZUqprYhdhaT9J3VjkpwHJ/qpgo Sty1-Tpx1 disulfide formation abrogated (Figure S1C). ------- COMMENT: 83cfcf15c86bcfbb 10 ThSBgYifm40404k7tPIZeJXRFyk Consistent with lower Sty1 activity, Sty15CS-expressing cells contained less Pyp1 than wild-type cells (Figure S1F). This likely explains the slightly elevated phosphorylation of Sty15CS (Figure S1G) ------- COMMENT: 83cfcf15c86bcfbb 11 VTCwgCY0XJASItVAUjjRQkPAD1U Sty15CS-expressing cells were able to adapt to os- motic stress but were less tolerant than wild-type cells to higher levels of H2O2 (Figure 1B). ------- COMMENT: 83cfcf15c86bcfbb 12 VTCwgCY0XJASItVAUjjRQkPAD1U Sty15CS-expressing cells were able to adapt to os- motic stress but were less tolerant than wild-type cells to higher levels of H2O2 (Figure 1B). ------- COMMENT: 83cfcf15c86bcfbb 13 iOP/qWfywvCB9vUVqXNTGKAbxoM Crucially, both Sty1-Tpx1 fu- sions were expressed at similar levels to wild-type Sty1 and sup- ported growth under stress conditions, confirming retention of Sty1 function (Figures 1B and 1D). By contrast, the oxidative stress sensitivity and lower Pyp1 levels in cells expressing a Sty15CS-Tpx1 fusion protein, provided further evidence that cys- teines in Sty1 are required for Sty1 function independently from forming disulfide-bonded complexes with Tpx1 (Figures 1B and S1F).39 ------- COMMENT: 83cfcf15c86bcfbb 14 /8puQaCN0MS0+DG2nr3YVgqi4SI Figure 1 Strikingly, Sty1 phosphorylation was increased in cells ex- pressing either Sty1-Tpx1 or Sty1-Tpx1C48S (Figure 1D) ------- COMMENT: 83cfcf15c86bcfbb 15 Lpura9CaeEIcX1uDWeWY5uXReEM This revealed that Sty1- Tpx1- and Sty1-Tpx1C48S-expressing cells both divided at a significantly smaller size than wild-type cells (Figure 1F). ------- COMMENT: 83cfcf15c86bcfbb 16 UWL65rmHuy9HP9CFVIbwSq0pbjU By contrast, cells expressing Sty1-Tpx1C48S con- tained substantially increased levels of lower mobility and phos- phorylated Pyp2 (p-Pyp2), even prior to addition of H2O2. Together these data strongly support the conclusion that the Sty1-Tpx1 and Sty1-Tpx1C48S fusion proteins are constitutively hyperactive compared with wild-type Sty1 (Figures 1F and 1G). ------- COMMENT: 83cfcf15c86bcfbb 18 ePsmAViEamlTPw5azW6rYXRyk0k Hence, the lower viability of cells co-expressing sty1-tpx1 and wis1DD suggested a synthetic negative interaction, with both alleles acting independently to increase Sty1 phosphorylation to lethal levels. ------- COMMENT: 83cfcf15c86bcfbb 19 1vd3CBueyOxWhza0vYdzDhSgP2o Indeed, our exam- ination indicated that ‘‘sty1-tpx1 wis1DD’’ strains, which genotyp- ically bore both alleles, had adapted to the deleterious effect of hyperactivated Sty1 by lowering Sty1 expression to such an extent that they exhibited the long cell phenotype associated with its loss (Figures S3A and S3B). ------- COMMENT: 83cfcf15c86bcfbb 20 q8MdVCEOZy80mx4JJhEWILJQuYc As expected, Sty1 phosphorylation was increased in Dpyp1 mutant cells, confirming the importance of this phosphatase in maintaining low levels of Sty1 activity (Fig- ure 2A) ------- COMMENT: 83cfcf15c86bcfbb 21 NrWfNOhrLECZmYDOXN8XZXMmNVc By contrast, over- expression of Tpx1 increased H2O2-induced Sty1 phosphoryla- tion in wild-type cells and also restored some H2O2-inducibility to Sty1 phosphorylation in Dpyp2 cells (Figures 2A and S3E). ------- COMMENT: 83cfcf15c86bcfbb 22 NrWfNOhrLECZmYDOXN8XZXMmNVc By contrast, over- expression of Tpx1 increased H2O2-induced Sty1 phosphoryla- tion in wild-type cells and also restored some H2O2-inducibility to Sty1 phosphorylation in Dpyp2 cells (Figures 2A and S3E). ------- COMMENT: 83cfcf15c86bcfbb 24 UnOvPNT3S4B022uIdsHL9rhTNRU Consistent with Pyp1 oxidation providing a similar mechanism to reversibly inhibit Pyp1 and activate Sty1, there was a much smaller H2O2-induced increase in Sty1 phos- phorylation in Dtrx1 mutant cells, where H2O2-induced Pyp1 disul- fides were not detected (Figures 2D and 2F). ------- COMMENT: 83cfcf15c86bcfbb 25 VV99kbWKsf9UpkCgkP8K6EDnKmw (comment: CHECK Tpx1 is required for H2O2-induced activa- tion of Sty1, over a range of concentrations up to 10 mM H2O2.31) ------- COMMENT: 83cfcf15c86bcfbb 26 mGLhKLxtflgh6WuHz09VkVJ2Ing Indeed, our analysis of Dtpx1 mutant cells indicated that Tpx1 was important for maximal Wis1 phosphorylation (pppWis1) in response to 6 mM H2O2 (Figures 3C, S4E, and S4F). ------- COMMENT: 83cfcf15c86bcfbb 28 75OTOiHHLsUSIOMzBzd4qrznI/o We observed a small, but reproducible, stress-induced decrease in Wis1DD mobility in these cells, corroborating that Wis1 undergoes a stress- induced phosphorylation on different sites from those phosphor- ylated by the MAP3K (Figures 4A, S5A, S4A, S4B, and S4F). ------- COMMENT: 83cfcf15c86bcfbb 29 XDj2HGdPIPlfMv5lR6DiCyuFM7c Significantly, this stress-induced increase in Wis1DD phosphory- lation was mirrored by stress-induced increases in Sty1 phos- phorylation, strongly suggesting that it increases Wis1 activity (Figure 4A). ------- COMMENT: 83cfcf15c86bcfbb 30 p8Be08QHbb4LA0mlUniQv7hql08 Next, we co-expressed the Sty1-Tpx1C48S fusion with a Wis1AA mutant, in which the MAP3K-phosphorylated residues are substituted with alanine. Wis1AA cells are significantly elongated, reflecting the mitotic delay associated with Sty1 hypophosphorylation (Figures 4B, 4C, and S6A).50 ------- COMMENT: 83cfcf15c86bcfbb 31 p8Be08QHbb4LA0mlUniQv7hql08 Next, we co-expressed the Sty1-Tpx1C48S fusion with a Wis1AA mutant, in which the MAP3K-phosphorylated residues are substituted with alanine. Wis1AA cells are significantly elongated, reflecting the mitotic delay associated with Sty1 hypophosphorylation (Figures 4B, 4C, and S6A).50 ------- COMMENT: 83cfcf15c86bcfbb 32 wwUmy1S7hx8l9R+7LZhtqOxNLK8 Although expression of Sty-Tpx1C48S stimulated a bigger increase in Sty1 activity in cells expressing wild-type Wis1, expression of Sty1-Tpx1C48S also increased Sty1 phosphorylation and partially rescued the cell-cycle defect of Wis1AA cells (Figures 4B and 4C) ------- COMMENT: 83cfcf15c86bcfbb 33 0b01M7lKXugI73EMf/zQQjwJOFg Although, both Wis1M395G and Wis1M395A were expressed at wild-type levels, Sty1 phosphory- lation was very low and minimally increased even in response to 6 mM H2O2 in these cells (Figure 4D). ------- COMMENT: 83cfcf15c86bcfbb 34 0b01M7lKXugI73EMf/zQQjwJOFg Although, both Wis1M395G and Wis1M395A were expressed at wild-type levels, Sty1 phosphory- lation was very low and minimally increased even in response to 6 mM H2O2 in these cells (Figure 4D). ------- COMMENT: 83cfcf15c86bcfbb 35 q+qerQJn0bEfzFICvypE2vE4HBM Strikingly, the stress- induced phosphorylation of Wis1M395G and Wis1M395A was also compromised, strongly suggesting that Wis1 kinase activity was required for the stress-induced phosphorylation and activation of Wis1 (Figure 4D). ------- COMMENT: 83cfcf15c86bcfbb 36 q+qerQJn0bEfzFICvypE2vE4HBM Strikingly, the stress- induced phosphorylation of Wis1M395G and Wis1M395A was also compromised, strongly suggesting that Wis1 kinase activity was required for the stress-induced phosphorylation and activation of Wis1 (Figure 4D). ------- COMMENT: 83cfcf15c86bcfbb 40 ZV1MaJjoBqJBGGwiE2PYqg8YFHs Dmcs4 mutant cells are delayed in entry to mitosis, reaching a significantly longer size than wild-type cells before dividing (Figure 5A).45,47,48 ------- COMMENT: 83cfcf15c86bcfbb 41 G8PBqyfLNkc50wZXrp9Th9KEjvM Sty1-Tpx1 expression stimulated similar levels of constitutive Wis1 phosphorylation in Dmcs4 mutant as in wild-type (mcs4+) cells (Figure 5B). Accordingly, there were similar levels of Sty1 phosphorylati ------- COMMENT: 83cfcf15c86bcfbb 42 i+CAWmXIqIQ7IrZeR+mLruj4W6M Sty1-Tpx1 complexes provide a scaffold for Wis1 ------- COMMENT: 83d3bee37f8b40fd 3 PtzKg9EE4D400cWirrQ9H31cNYc Biochemical and mutagenic studies demonstrated that the [2Fe-2S]2+ cluster substantially inhibits the phosphatase activity of Asp1, thereby increasing its net kinase activity. ------- COMMENT: 83dde591569634d9 1 CQLQRu5r83zX12CD1Y5KJO1rLtM (comment: CHECK in vitro kinase assay using recombinant Sre1 aa 1-440) ------- COMMENT: 83dde591569634d9 2 hvzhOuD5vfa1l4y3boq5+EPwKSw Binds specifically to active Sre1 transcription factor and not full-length precursor ------- COMMENT: 83dde591569634d9 4 7WpA8Zi9dc3pKmXtyKStfvnIBk0 accelerates degradation of active Sre1 transcription factor ------- COMMENT: 83dde591569634d9 5 561HdpNY0lagmg1E5IbVSpkpnSc hhp2 deletion increases steady-state ergosterol ------- COMMENT: 83dde591569634d9 12 NTDFywNImk0LEEOEwuUAwE9+2+s (comment: Ok as a single mutant despite sre1-N mutant?) ------- COMMENT: 845e17d046d8c896 2 /MIV9twFYYkcNXGdEvJ3DjBbsF8 (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 3 /MIV9twFYYkcNXGdEvJ3DjBbsF8 (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 8 /MIV9twFYYkcNXGdEvJ3DjBbsF8 (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 9 A0rveV668ozD+BYHquABtC3xGnA (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 10 A0rveV668ozD+BYHquABtC3xGnA (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 11 A0rveV668ozD+BYHquABtC3xGnA (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 13 n5UvxDaXIvix+UAxLjLH3tM0gzw (comment: ade6B/ade6X at the ura4 locus) ------- COMMENT: 845e17d046d8c896 15 /MIV9twFYYkcNXGdEvJ3DjBbsF8 (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 17 n5UvxDaXIvix+UAxLjLH3tM0gzw (comment: ade6B/ade6X at the ura4 locus) ------- COMMENT: 845e17d046d8c896 20 /MIV9twFYYkcNXGdEvJ3DjBbsF8 (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 22 /MIV9twFYYkcNXGdEvJ3DjBbsF8 (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 23 /MIV9twFYYkcNXGdEvJ3DjBbsF8 (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 24 /MIV9twFYYkcNXGdEvJ3DjBbsF8 (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 25 n5UvxDaXIvix+UAxLjLH3tM0gzw (comment: ade6B/ade6X at the ura4 locus) ------- COMMENT: 845e17d046d8c896 26 /MIV9twFYYkcNXGdEvJ3DjBbsF8 (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 27 /MIV9twFYYkcNXGdEvJ3DjBbsF8 (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 29 89GkrXYO1vtzFQxybbY24x9TsKg (comment: ade6B/ade6X at cen1) ------- COMMENT: 845e17d046d8c896 30 89GkrXYO1vtzFQxybbY24x9TsKg (comment: ade6B/ade6X at cen1) ------- COMMENT: 845e17d046d8c896 31 89GkrXYO1vtzFQxybbY24x9TsKg (comment: ade6B/ade6X at cen1) ------- COMMENT: 845e17d046d8c896 32 n5UvxDaXIvix+UAxLjLH3tM0gzw (comment: ade6B/ade6X at the ura4 locus) ------- COMMENT: 845e17d046d8c896 33 n5UvxDaXIvix+UAxLjLH3tM0gzw ade6B/ade6X at the ura4 locus ------- COMMENT: 845e17d046d8c896 34 n5UvxDaXIvix+UAxLjLH3tM0gzw (comment: ade6B/ade6X at the ura4 locus) ------- COMMENT: 845e17d046d8c896 36 89GkrXYO1vtzFQxybbY24x9TsKg (comment: ade6B/ade6X at cen1) ------- COMMENT: 845e17d046d8c896 37 89GkrXYO1vtzFQxybbY24x9TsKg (comment: ade6B/ade6X at cen1) ------- COMMENT: 845e17d046d8c896 38 89GkrXYO1vtzFQxybbY24x9TsKg (comment: ade6B/ade6X at cen1) ------- COMMENT: 845e17d046d8c896 39 89GkrXYO1vtzFQxybbY24x9TsKg a(comment: de6B/ade6X at cen1) ------- COMMENT: 845e17d046d8c896 40 89GkrXYO1vtzFQxybbY24x9TsKg (comment: ade6B/ade6X at cen1) ------- COMMENT: 845e17d046d8c896 41 89GkrXYO1vtzFQxybbY24x9TsKg (comment: ade6B/ade6X at cen1) ------- COMMENT: 845e17d046d8c896 42 89GkrXYO1vtzFQxybbY24x9TsKg (comment: ade6B/ade6X at cen1) ------- COMMENT: 845e17d046d8c896 43 89GkrXYO1vtzFQxybbY24x9TsKg (comment: ade6B/ade6X at cen1) ------- COMMENT: 845e17d046d8c896 44 89GkrXYO1vtzFQxybbY24x9TsKg (comment: ade6B/ade6X at cen1) ------- COMMENT: 845e17d046d8c896 45 89GkrXYO1vtzFQxybbY24x9TsKg (comment: ade6B/ade6X at cen1) ------- COMMENT: 845e17d046d8c896 46 89GkrXYO1vtzFQxybbY24x9TsKg (comment: ade6B/ade6X at cen1) ------- COMMENT: 845e17d046d8c896 47 89GkrXYO1vtzFQxybbY24x9TsKg (comment: ade6B/ade6X at cen1) ------- COMMENT: 845e17d046d8c896 48 A0rveV668ozD+BYHquABtC3xGnA (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 49 A0rveV668ozD+BYHquABtC3xGnA (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 50 /MIV9twFYYkcNXGdEvJ3DjBbsF8 (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 51 /MIV9twFYYkcNXGdEvJ3DjBbsF8 (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 52 /MIV9twFYYkcNXGdEvJ3DjBbsF8 (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 53 /MIV9twFYYkcNXGdEvJ3DjBbsF8 (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 54 /MIV9twFYYkcNXGdEvJ3DjBbsF8 (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 55 n5UvxDaXIvix+UAxLjLH3tM0gzw (comment: ade6B/ade6X at the ura4 locus) ------- COMMENT: 845e17d046d8c896 56 /MIV9twFYYkcNXGdEvJ3DjBbsF8 (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 58 n5UvxDaXIvix+UAxLjLH3tM0gzw (comment: ade6B/ade6X at the ura4 locus) ------- COMMENT: 845e17d046d8c896 60 /MIV9twFYYkcNXGdEvJ3DjBbsF8 (comment: An extrachromosome ChLC) ------- COMMENT: 845e17d046d8c896 61 /MIV9twFYYkcNXGdEvJ3DjBbsF8 (comment: An extrachromosome ChLC) ------- COMMENT: 847e79ae2982da4f 1 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 847e79ae2982da4f 2 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 847e79ae2982da4f 3 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 847e79ae2982da4f 4 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 847e79ae2982da4f 5 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 847e79ae2982da4f 6 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 847e79ae2982da4f 7 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 847e79ae2982da4f 8 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 847e79ae2982da4f 11 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 847e79ae2982da4f 12 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 847e79ae2982da4f 13 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 847e79ae2982da4f 14 zQnvuu0SnwMLEjcLwjUToskAFbo Figure S4A, B, C ------- COMMENT: 847e79ae2982da4f 15 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 847e79ae2982da4f 16 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 847e79ae2982da4f 17 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 847e79ae2982da4f 18 Q2Xir0MupXiFT1sAqDJn+lGAAnQ Figure 2A Both the cytb and cox2 mRNAs were present at normal levels in the Δcbp6 mutant ------- COMMENT: 847e79ae2982da4f 19 Q2Xir0MupXiFT1sAqDJn+lGAAnQ Figure 2A Both the cytb and cox2 mRNAs were present at normal levels in the Δcbp6 mutant ------- COMMENT: 847e79ae2982da4f 20 QIeSntWWMepnVECSgosaIROUmu4 Figure 2C Thus, virtually all of the Cytb protein synthesized in the Δcbp6 mutant is degraded ------- COMMENT: 847e79ae2982da4f 21 AB4TGlDFCx3anE7tS7ehGuForcw Figure 2D In both Δcbp6 and control Δcytb mitochondria, complex III was completely lacking (lanes 3 and 4) ------- COMMENT: 847e79ae2982da4f 22 GXjtjpPz0MTmweTrzZJBjXKqlBQ absent respiratory complex III Figure 2D In both Δcbp6 and control Δcytb mitochondria, complex III was completely lacking (lanes 3 and 4) ------- COMMENT: 847e79ae2982da4f 23 eQVNAriazO7XMfuSf6oQ9rBUlDQ absent respiratory complex III. Figure 2D As expected, the higher molecular weight bands of complex III were absent in the Δppr4 mutant, which lacks complex IV ------- COMMENT: 847e79ae2982da4f 24 DvKDNSsUqRPkYRntLMD+xDz4yd4 The tagged Mss51 was detected only in the mitochondrial fraction and like Cox2 was strongly resistant to carbonate extraction (Figure 4A), indicating that it is a membrane protein. ------- COMMENT: 847e79ae2982da4f 25 D7as8L9ELc0VKyHLDYOU/S++/Vc (Figure 4B) A deletion mutants showed a clear growth defect on galactose medium ------- COMMENT: 847e79ae2982da4f 26 x3fuOkyyZgJNROg4Zkk28i6X4II Similarly to the Δcbp3 and Δcbp6 mutants, Δmss51 cells were resistant to antimycin A on glucose medium, showing that they contain a functional complex V ------- COMMENT: 847e79ae2982da4f 30 OGsIzyPzHygB+Ubd4HUnyrFWaQM Thus, in S. pombe, Mss51 appears to be required at a post-translational step of complex IV biogenesis ------- COMMENT: 847e79ae2982da4f 33 +NWc/eLKCRhb5JGME/VVXhhYkAQ Δmss51 cells showed normal cytochrome b and c1 peaks, but cytochromes aa3 were not detectable. ------- COMMENT: 847e79ae2982da4f 34 QOTCmxGgfYWyGithtQBLgUjlhIQ Cytb, Cox1, 2 and 3 were clearly visible, although Cox2 was less strongly labeled in both mutants, especially Δmss51 (Figure 5A) ------- COMMENT: 847e79ae2982da4f 35 QOTCmxGgfYWyGithtQBLgUjlhIQ Cytb, Cox1, 2 and 3 were clearly visible, although Cox2 was less strongly labeled in both mutants, especially Δmss51 (Figure 5A) ------- COMMENT: 847e79ae2982da4f 36 QOTCmxGgfYWyGithtQBLgUjlhIQ Cytb, Cox1, 2 and 3 were clearly visible, although Cox2 was less strongly labeled in both mutants, especially Δmss51 (Figure 5A) ------- COMMENT: 847e79ae2982da4f 37 hntAfntPW7BADZif6OMzJlEaOso As expected, Δppr4 cells clearly lacked Cox1 ------- COMMENT: 847e79ae2982da4f 38 ZDJylbBYvc1Rmk0ryyyPZuzU0HY Northern blots revealed no defect in the accumulation of mature messengers (Figure 5B) ------- COMMENT: 847e79ae2982da4f 39 ZDJylbBYvc1Rmk0ryyyPZuzU0HY Northern blots revealed no defect in the accumulation of mature messengers (Figure 5B) ------- COMMENT: 847e79ae2982da4f 40 fCj+ZRIlpJswyKVqgG0lB/KCGAA Cox2 was detectable in Δmss51 purified mitochondria, although its level was greatly reduced (Figure 5C), consistent with the reduced 35S labeling (Figure 5A) ------- COMMENT: 847e79ae2982da4f 41 OGgE11TjB4Ay9nL5+0hrIIDnB/Y Cox1 was clearly less stable in the Δmss51 mutant than in the wild- type, while Cox2 was poorly labeled in the mutant even before starting the chase, as noted before (Figure 5C). ------- COMMENT: 847e79ae2982da4f 42 fOtoaXyhmUGmVAhfe2+kB2F1fq4 Figure 5E ------- COMMENT: 8481a1a5bf90b23e 33 /YxBlI3s4aVADjGFRy1QJF6JtLY (comment: selective for phosphorylated Spt5) ------- COMMENT: 848fdf36e609c892 1 ekDPQHbAnbe6qq1nVLc0Qsg/Ow0 Fig1A ------- COMMENT: 848fdf36e609c892 2 +KzOGwgSY4WZ0rdmAhmVGAoSPuw Fig1B, C Table 1 ------- COMMENT: 848fdf36e609c892 3 +KzOGwgSY4WZ0rdmAhmVGAoSPuw Fig1B, C Table 1 ------- COMMENT: 848fdf36e609c892 4 +KzOGwgSY4WZ0rdmAhmVGAoSPuw Fig1B, C Table 1 ------- COMMENT: 848fdf36e609c892 6 +KzOGwgSY4WZ0rdmAhmVGAoSPuw Fig1B, C Table 1 ------- COMMENT: 848fdf36e609c892 7 +KzOGwgSY4WZ0rdmAhmVGAoSPuw Fig1B, C Table 1 ------- COMMENT: 848fdf36e609c892 9 77IpUt4TE/By2admuY1ipBfTK1Q Fig 2, Table 2 ------- COMMENT: 848fdf36e609c892 10 77IpUt4TE/By2admuY1ipBfTK1Q Fig 2, Table 2 ------- COMMENT: 848fdf36e609c892 11 IzYOyl7GlRXfCCuGRWctPeIOaRs Fig3A-F ((comment: same result in wild type background) ------- COMMENT: 848fdf36e609c892 12 NRez/fhTpyE1UMh4DAY3Deuiwf4 Fig 3A-F (comment: same result in Wild type background) ------- COMMENT: 848fdf36e609c892 13 JeaJiJimTJga5fZ38swARui5mH0 Fig3H (comment: same in wild type background) ------- COMMENT: 848fdf36e609c892 14 p4WWFYyG0bKg3chHlcWiI4W0wVI Fig 3I (comment: same in wild type background) ------- COMMENT: 848fdf36e609c892 15 PXqQ8w7WZKoenOmS6L1/H8ouUb4 Fig4A ------- COMMENT: 848fdf36e609c892 16 3xSe9DcbTqbUSJZ2IdyjdHZwfYA FigS1. delayed septation, Cellular phenotype where cells initiate growth before septation has taken place, resulting in variable cell size at division. ------- COMMENT: 848fdf36e609c892 18 1rPAo6EnWDTbsi1qTzrdEcitx/Y Fig4 B,C, D. A cellular phenotype found in multinucleated cells where nuclear division is no longer synchronous and cells with an odd number of nuclei are observed ------- COMMENT: 848fdf36e609c892 19 s8zxygB4j9+cKrsniSSlYhY+9P0 Fig5A ------- COMMENT: 848fdf36e609c892 20 s8zxygB4j9+cKrsniSSlYhY+9P0 Fig5A ------- COMMENT: 848fdf36e609c892 21 Ki0j1l7+B+8jMHI1qS2WusBgT78 Fig5C compare 4 and 6 the variability of cdc2cdc13 fusion protein is increased in mga2 delta. I don't quite know how to annotate this or whether I leave it as 'variable size at division and do a genetic interaction and Im not sure that the term I have suggested is right ------- COMMENT: 848fdf36e609c892 23 QhOwdPjdEbD70m0jusS5Lhknb2k Fig5C compare 7 and 9 When cdc2 is not tyrosine phosphorylation mga2 delta does not increase the cell size variability any further. ------- COMMENT: 848fdf36e609c892 24 SV7UvCpNK1YcaF1vWJH5as6jM0Q cellular phenotype of variable cell size at division may be further increased in the absence of another cellular protein Fig5 compare 14 and 15 Table 3 I don't quite know how to annotate this or whether I leave it as 'variable size at division and do a genetic interaction and Im not sure that the term I have suggested is right ------- COMMENT: 848fdf36e609c892 25 NYlQ8vqJhOSubNVY59LaP79yJuI Fig5C compare16 and 17 Table 3 I don't quite know how to annotate this or whether I leave it as 'variable size at division and do a genetic interaction and Im not sure that the term I have suggested is right ------- COMMENT: 848fdf36e609c892 26 51HlnZbGtMuBU59n+SOxA58JU5E Fig5C Table 3 ------- COMMENT: 848fdf36e609c892 27 n1WmqphYx0SiTXdAUnW51ChHFtk Fig5 E,F abnormal protein localisation in multinucleated cells ------- COMMENT: 848fdf36e609c892 28 s8zxygB4j9+cKrsniSSlYhY+9P0 Fig5A ------- COMMENT: 848fdf36e609c892 29 zJsAmxUY89Pm7QE++ySEwAePpnQ Fig4A. ------- COMMENT: 8490fc7b048193c4 1 I3URoPQhS9O6ycONSoHEMAVaA9Q Based on these results, we propose a model for the early step of the strand exchange reaction involving Rhp51 and the two mediators. Rad22 recruits Rhp51 to RPA-coated ssDNA. ------- COMMENT: 84cf2fbfe26627cc 2 TwTonNPNiRC9Of2r+GBDmgdw4eM ------- COMMENT: 84cf2fbfe26627cc 3 ZYSL/MHFXi4rifp+VBNCAdgFeh4 (comment: transcription read through by PCR) ------- COMMENT: 84cf2fbfe26627cc 4 Gsm1uKscABH8MqOTOn3eg6JYLZI (comment: CHECK RT-PCR) ------- COMMENT: 84cf2fbfe26627cc 8 ZYSL/MHFXi4rifp+VBNCAdgFeh4 (comment: transcription read through by PCR) ------- COMMENT: 84dc6f3caefc7153 2 RUWn8+yjGJBggtM4wQ0ldbT43gQ (comment: CHECK SLD1) ------- COMMENT: 84dc6f3caefc7153 3 pS8pdmfFLvmXCsXkU+Z1vdJtyaA Strikingly, a GST-Rad60 SLD2E380R construct did not detectably interact with Ubc9-TAP (Fig. 3a), ------- COMMENT: 84dc6f3caefc7153 6 LRnPQaJu3AxHwxcBryuEKpQxPfw slow growth phenotype and heterogeneity in cell length; reflecting elevated levels of spontaneous DNA damage and “constitutive” activation of the DNA damage checkpoint in these cells (Fig. 4a and data not shown). ------- COMMENT: 84dc6f3caefc7153 7 LRnPQaJu3AxHwxcBryuEKpQxPfw slow growth phenotype and heterogeneity in cell length; reflecting elevated levels of spontaneous DNA damage and “constitutive” activation of the DNA damage checkpoint in these cells (Fig. 4a and data not shown). ------- COMMENT: 84dc6f3caefc7153 8 LRnPQaJu3AxHwxcBryuEKpQxPfw slow growth phenotype and heterogeneity in cell length; reflecting elevated levels of spontaneous DNA damage and “constitutive” activation of the DNA damage checkpoint in these cells (Fig. 4a and data not shown). ------- COMMENT: 84dc6f3caefc7153 11 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 84dc6f3caefc7153 12 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 84dc6f3caefc7153 13 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 84dc6f3caefc7153 14 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 84dc6f3caefc7153 15 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 84dc6f3caefc7153 16 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 84dc6f3caefc7153 17 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 84dc6f3caefc7153 18 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 84dc6f3caefc7153 19 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 84dcea5bf4f580d2 1 7gso0Lknn+iu27KO1FbYdRKWHwM Pap1 is ubiquitylated by Rhp6 and Ubr1 ------- COMMENT: 84dcea5bf4f580d2 2 7gso0Lknn+iu27KO1FbYdRKWHwM Pap1 is ubiquitylated by Rhp6 and Ubr1 ------- COMMENT: 84dcea5bf4f580d2 67 rFUsNf9WD7lncl9cYXAoJiBuRjw MBC resistance phenotype and pap1 ubiquitylation phenotype ------- COMMENT: 84dcea5bf4f580d2 68 dqRGOREUpsPOK4BUWXlLpQMFp20 MBC sensitivity phenotype and ubiquitylation and degradation phenotype ------- COMMENT: 84dcea5bf4f580d2 70 rFUsNf9WD7lncl9cYXAoJiBuRjw MBC resistance phenotype and pap1 ubiquitylation phenotype ------- COMMENT: 84dcea5bf4f580d2 71 dqRGOREUpsPOK4BUWXlLpQMFp20 MBC sensitivity phenotype and ubiquitylation and degradation phenotype ------- COMMENT: 84f0f5ee8d42bdac 2 xm2ofQmTLiTIBaTSH0nJwGqPn6c figS2A ------- COMMENT: 84f0f5ee8d42bdac 3 lD9a3Mm/dLRixe5LYihCtpLx2Mw fig1E ------- COMMENT: 84f0f5ee8d42bdac 8 lD9a3Mm/dLRixe5LYihCtpLx2Mw fig1E ------- COMMENT: 84f0f5ee8d42bdac 9 lD9a3Mm/dLRixe5LYihCtpLx2Mw fig1E ------- COMMENT: 84f0f5ee8d42bdac 10 GKf6tV7oqFclFsVFJi7uNThL+Fo fig1F ------- COMMENT: 84f0f5ee8d42bdac 11 GKf6tV7oqFclFsVFJi7uNThL+Fo fig1F ------- COMMENT: 84f0f5ee8d42bdac 15 rz4HawhEFv4YVXGYmGTAwGoamQU fig1F/figS2A ------- COMMENT: 84f0f5ee8d42bdac 16 GKf6tV7oqFclFsVFJi7uNThL+Fo fig1F ------- COMMENT: 84f0f5ee8d42bdac 19 gbsEZDL2WsTbMBJ8nTkh0vGNYlA fig 2A ------- COMMENT: 84f0f5ee8d42bdac 20 gbsEZDL2WsTbMBJ8nTkh0vGNYlA fig 2A ------- COMMENT: 84f0f5ee8d42bdac 21 60ysKND9FJM88kC14cRKIvzlGGM figure 2c Figures 3B and S3B Figures 4C and 4D ------- COMMENT: 84f0f5ee8d42bdac 22 60ysKND9FJM88kC14cRKIvzlGGM figure 2c Figures 3B and S3B Figures 4C and 4D ------- COMMENT: 84f0f5ee8d42bdac 23 q8fDfLFBYviVRffPRG0km1LMRcI Figures 4E and 4F ------- COMMENT: 84f0f5ee8d42bdac 24 q8fDfLFBYviVRffPRG0km1LMRcI Figures 4E and 4F ------- COMMENT: 84f0f5ee8d42bdac 25 q8fDfLFBYviVRffPRG0km1LMRcI Figures 4E and 4F ------- COMMENT: 85219fa3fd50b9ee 28 Ohh5/taKUUUjvohRPPgF1v9FTYc ------- COMMENT: 85219fa3fd50b9ee 30 Ohh5/taKUUUjvohRPPgF1v9FTYc ------- COMMENT: 85219fa3fd50b9ee 33 Ohh5/taKUUUjvohRPPgF1v9FTYc ------- COMMENT: 85219fa3fd50b9ee 35 Ohh5/taKUUUjvohRPPgF1v9FTYc ------- COMMENT: 855fbb0238fc409f 3 wK0QljG6r4JTmws2boTgfkX+Zo0 (Fig. 4C and 4E) ------- COMMENT: 855fbb0238fc409f 4 wK0QljG6r4JTmws2boTgfkX+Zo0 (Fig. 4C and 4E) ------- COMMENT: 855fbb0238fc409f 6 SvlFFz9CaCJByQRBTLvdFnPqSYg (Fig. 1F) ------- COMMENT: 855fbb0238fc409f 7 wK0QljG6r4JTmws2boTgfkX+Zo0 (Fig. 4C and 4E) ------- COMMENT: 855fbb0238fc409f 8 SvlFFz9CaCJByQRBTLvdFnPqSYg (Fig. 1F) ------- COMMENT: 855fbb0238fc409f 9 SvlFFz9CaCJByQRBTLvdFnPqSYg (Fig. 1F) ------- COMMENT: 855fbb0238fc409f 10 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: 855fbb0238fc409f 11 xF1NHfVTsuFLAECwYSMLt7u5f4M (Fig. 4C, 4D and 4E) ------- COMMENT: 855fbb0238fc409f 12 +VNR+ja40el0beIXEpBKIHxV8Sk (Fig. 4D and 4E) ------- COMMENT: 855fbb0238fc409f 13 +VNR+ja40el0beIXEpBKIHxV8Sk (Fig. 4D and 4E) ------- COMMENT: 855fbb0238fc409f 14 +VNR+ja40el0beIXEpBKIHxV8Sk (Fig. 4D and 4E) ------- COMMENT: 855fbb0238fc409f 15 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: 855fbb0238fc409f 16 p3x2dDTjQlS9jbO+IMHpHbED4nw (Fig. 1D) ------- COMMENT: 855fbb0238fc409f 17 p3x2dDTjQlS9jbO+IMHpHbED4nw (Fig. 1D) ------- COMMENT: 855fbb0238fc409f 18 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: 855fbb0238fc409f 19 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: 855fbb0238fc409f 20 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: 855fbb0238fc409f 21 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: 855fbb0238fc409f 22 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: 855fbb0238fc409f 23 UecLDdbzEF2Fd34z5as/4/0I/eg (Fig. S1E) ------- COMMENT: 8570af08389340e2 33 LLc/UbXMA+Keib2btioGY9sBGNA Consistent with this, we observed that the nuclear envelope formed thin protrusions that grew and shrank (Figure 4, G and H, Supplementary Movie 8). Such intranuclear MTs and nuclear envelope distortions were notobserved in wild-type cells. Several criteria confirmed that these cells with intranuclear MTs were in interphase and not in mitosis ------- COMMENT: 8570af08389340e2 41 vapiw+Zhy3P9ORnRiVCJXYypBO8 (comment: normal length) ------- COMMENT: 8589306346786b20 4 XZXDTYAhNEp+TNSh6KDQwc3ugOM (comment: Gaf1-GFP is found in the nucleus following nitrogen starvation but not glucose starvation) ------- COMMENT: 8589306346786b20 8 tgoJNUwk1I8Krh8gGshOvISP5B8 (comment: CONDITION 1h in proline medium) ------- COMMENT: 8589306346786b20 9 glXdRs606pDDVxwpiK99YdwEM78 ------- COMMENT: 8589306346786b20 10 glXdRs606pDDVxwpiK99YdwEM78 ------- COMMENT: 8589306346786b20 12 glXdRs606pDDVxwpiK99YdwEM78 ------- COMMENT: 8589306346786b20 13 l0g8SCDHjiVJdyMxsIgyu0+ZVpw (comment: CONDITION 1h in proline medium) (comment: CHECK a mild phenotype) ------- COMMENT: 8589306346786b20 14 l0g8SCDHjiVJdyMxsIgyu0+ZVpw (comment: CONDITION 1h in proline medium) (comment: CHECK a mild phenotype) ------- COMMENT: 8589306346786b20 15 l0g8SCDHjiVJdyMxsIgyu0+ZVpw (comment: CONDITION 1h in proline medium) (comment: CHECK a mild phenotype) ------- COMMENT: 8589306346786b20 16 DJFcizWz0SnvxIZusurzcdGdis4 (comment: CHECK a mild phenotype) ------- COMMENT: 8589306346786b20 17 WIQobKVmOSwF4pnw3zyHiiYk2Lk fig1a ------- COMMENT: 8589306346786b20 18 WIQobKVmOSwF4pnw3zyHiiYk2Lk fig1a ------- COMMENT: 8589306346786b20 19 WIQobKVmOSwF4pnw3zyHiiYk2Lk fig1a ------- COMMENT: 8589306346786b20 20 WIQobKVmOSwF4pnw3zyHiiYk2Lk fig1a ------- COMMENT: 8589306346786b20 21 WIQobKVmOSwF4pnw3zyHiiYk2Lk fig1a ------- COMMENT: 8589306346786b20 22 WIQobKVmOSwF4pnw3zyHiiYk2Lk fig1a ------- COMMENT: 8589306346786b20 23 WIQobKVmOSwF4pnw3zyHiiYk2Lk fig1a ------- COMMENT: 8589306346786b20 24 WIQobKVmOSwF4pnw3zyHiiYk2Lk fig1a ------- COMMENT: 8589306346786b20 25 WIQobKVmOSwF4pnw3zyHiiYk2Lk fig1a ------- COMMENT: 8589306346786b20 33 XZXDTYAhNEp+TNSh6KDQwc3ugOM (comment: Gaf1-GFP is found in the nucleus following nitrogen starvation but not glucose starvation) ------- COMMENT: 8589306346786b20 36 DJFcizWz0SnvxIZusurzcdGdis4 (comment: CHECK a mild phenotype) ------- COMMENT: 8589306346786b20 37 glXdRs606pDDVxwpiK99YdwEM78 ------- COMMENT: 8589306346786b20 41 glXdRs606pDDVxwpiK99YdwEM78 ------- COMMENT: 8589306346786b20 42 glXdRs606pDDVxwpiK99YdwEM78 ------- COMMENT: 8589306346786b20 43 glXdRs606pDDVxwpiK99YdwEM78 ------- COMMENT: 8589306346786b20 44 glXdRs606pDDVxwpiK99YdwEM78 ------- COMMENT: 858ecbd8337e9ead 1 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 858ecbd8337e9ead 2 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 858ecbd8337e9ead 3 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 858ecbd8337e9ead 4 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 858ecbd8337e9ead 5 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 858ecbd8337e9ead 6 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 85b46136eb31ff35 10 5d4EJ3/XXAYJehrdv2wUgNbbhzc (comment: salt stres) ------- COMMENT: 85b46136eb31ff35 14 5d4EJ3/XXAYJehrdv2wUgNbbhzc (comment: salt stress) ------- COMMENT: 85bcd881821a0d07 8 ic3BJrocotT6E/Qg9TC4IYDWef0 ------- COMMENT: 85bcd881821a0d07 9 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: 85bcd881821a0d07 20 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: 85bcd881821a0d07 22 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: 85bcd881821a0d07 25 deQWSPIrLu3JNbLj6To9/eL5abQ Fig. 1EF ------- COMMENT: 85bcd881821a0d07 26 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: 85bcd881821a0d07 27 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: 85bcd881821a0d07 29 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: 85bcd881821a0d07 30 r/R8vI6TkLOmQr47F7QOhGGegy4 Figure 3b ------- COMMENT: 85bcd881821a0d07 31 r/R8vI6TkLOmQr47F7QOhGGegy4 Figure 3b ------- COMMENT: 85bcd881821a0d07 35 6ETquB6qf7XZAg8RDXUoPTp/g3Q Fig 6A ------- COMMENT: 85bcd881821a0d07 36 7ORt3I/HK4LBUYz+zRL2eYwvW0w Fig 6C ------- COMMENT: 85bcd881821a0d07 37 7ORt3I/HK4LBUYz+zRL2eYwvW0w Fig 6C ------- COMMENT: 85bcd881821a0d07 38 ZFoc+FAMeGMUiqu3YsX7C2wdfqM Figure 6EF ------- COMMENT: 85bcd881821a0d07 39 uibya5DFuhoEFRLoKNiZPm/fAW4 (Fig. 6G). ------- COMMENT: 85bcd881821a0d07 40 tc3rJxZshAX+tmBq7BR9TkADO4o Fig 7A ------- COMMENT: 85bcd881821a0d07 41 tc3rJxZshAX+tmBq7BR9TkADO4o Fig 7A ------- COMMENT: 85bcd881821a0d07 42 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 85bcd881821a0d07 43 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 85bcd881821a0d07 46 j0sFwPNhhHJ/lr9E3wNigYLUxSM figure 1D ------- COMMENT: 85e293d86887ff6e 1 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 85e293d86887ff6e 2 a8RbYPxyb4TryQgXmYU92FlHfQM Fig. 1A and B ------- COMMENT: 85e293d86887ff6e 3 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 85e293d86887ff6e 4 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 85e293d86887ff6e 5 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 85e293d86887ff6e 6 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 85e293d86887ff6e 7 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 85e293d86887ff6e 8 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 85e293d86887ff6e 9 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 85e293d86887ff6e 10 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 85e293d86887ff6e 11 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 85e293d86887ff6e 12 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 85e293d86887ff6e 13 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 85e293d86887ff6e 14 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 85e293d86887ff6e 15 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 85e293d86887ff6e 16 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 85e293d86887ff6e 17 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 85e293d86887ff6e 18 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 85e293d86887ff6e 19 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 85e293d86887ff6e 20 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 85e293d86887ff6e 21 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 85e293d86887ff6e 22 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 85e293d86887ff6e 23 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 85e293d86887ff6e 24 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 85e293d86887ff6e 25 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 85e293d86887ff6e 26 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 85e293d86887ff6e 27 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 85e293d86887ff6e 28 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 85e293d86887ff6e 29 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 85e293d86887ff6e 30 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 85e293d86887ff6e 31 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 85e293d86887ff6e 32 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 85e293d86887ff6e 33 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 85e293d86887ff6e 34 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 85e293d86887ff6e 35 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 85e293d86887ff6e 36 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 85e293d86887ff6e 37 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 85e293d86887ff6e 38 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 85e293d86887ff6e 39 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 85e293d86887ff6e 40 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 85e293d86887ff6e 41 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 85e293d86887ff6e 42 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 85e293d86887ff6e 43 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 85e293d86887ff6e 44 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 85e293d86887ff6e 45 LR03zDKtXw9LS9E+z8jX9MTPg+E Fig. 5A and B ------- COMMENT: 85e293d86887ff6e 46 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 85e293d86887ff6e 47 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 85e293d86887ff6e 48 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 85e293d86887ff6e 49 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 85e293d86887ff6e 50 xLld+U4XtO1Yq5XJ/vA+lPgihjU Fig. 6D ------- COMMENT: 85e293d86887ff6e 51 xLld+U4XtO1Yq5XJ/vA+lPgihjU Fig. 6D ------- COMMENT: 85e6122e0094ad3d 1 7jTMslpcfUj2lA5jgmWoBbN1ag4 (comment: cryptic dihydroorotase domain) ------- COMMENT: 865ebc50ec806a07 10 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: 865ebc50ec806a07 11 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: 865ebc50ec806a07 12 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 865ebc50ec806a07 13 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 865ebc50ec806a07 14 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 865ebc50ec806a07 15 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 865ebc50ec806a07 16 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 865ebc50ec806a07 17 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 865ebc50ec806a07 18 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 865ebc50ec806a07 19 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 865ebc50ec806a07 20 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 865ebc50ec806a07 21 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 865ebc50ec806a07 22 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 865ebc50ec806a07 23 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 865ebc50ec806a07 24 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 865ebc50ec806a07 25 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 865ebc50ec806a07 26 fYqQOpzikopc+3AWAEPEUkYII7w figure 4 ------- COMMENT: 865ebc50ec806a07 27 fYqQOpzikopc+3AWAEPEUkYII7w figure 4 ------- COMMENT: 865ebc50ec806a07 28 fYqQOpzikopc+3AWAEPEUkYII7w figure 4 ------- COMMENT: 865ebc50ec806a07 29 fYqQOpzikopc+3AWAEPEUkYII7w figure 4 ------- COMMENT: 865ebc50ec806a07 30 fYqQOpzikopc+3AWAEPEUkYII7w figure 4 ------- COMMENT: 865ebc50ec806a07 31 zZPPrIAJVHgTFjPOw7lZioI06ZA figure 4F ------- COMMENT: 865ebc50ec806a07 32 fYqQOpzikopc+3AWAEPEUkYII7w figure 4 ------- COMMENT: 865ebc50ec806a07 33 fYqQOpzikopc+3AWAEPEUkYII7w figure 4 ------- COMMENT: 86f3e1c248376b23 3 FW5RcZ6EFIuf7CBdulUpeCaMLDw ((comment: severity correlates positively with overexpression level) ------- COMMENT: 86f3e1c248376b23 4 jO3nkoq4IfODJ6woXuGeiZmiqYU (comment: severity correlates positively with overexpression level, and different isolates with same construct integrated show different Cdc18 level) ------- COMMENT: 86f3e1c248376b23 16 Qz/2v4Hb+5VU1rVA4lwDCPUg96s (comment: cdc18+ low level overexpression) ------- COMMENT: 86f72e4a0d568a17 6 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 86f72e4a0d568a17 7 o4Zv40MWp+EuQEggZ8qvp7Di/R0 FACS analysis of germinating haploid cdc13delta spores. Up to 32C DNA content was observed by 19 hour after referring spores to allow germination Figure 2 ------- COMMENT: 86f72e4a0d568a17 8 Gx6vqNWRS2BOE4V17R98K4nm4FQ germinating haploid cdc13delta spores, observed by 14 hour after spores allowed to germination (Figure 2b). cdc13delete cells kept alive by a multicopy plasmid, pSM2-cdc13. Cell phenotype was observed after plasmid loss same phenotype as in Figure 2b ------- COMMENT: 86f72e4a0d568a17 9 ZHh7Jk+8tOBcsz8gHIx0+STZQfc p34cdc2 cdc13-9 complex was pulled down using p13suc1 beads and then western blotted using anti cdc13 antibody SP4 to show reduced levels of Cdc13 complexed with cdc2 compared to the wild type control ------- COMMENT: 86f72e4a0d568a17 11 VKfLyWEObSbyLUogoEjpWLEjc1Q cdc10-129 cells blocked in G1 over expressing cdc2 from integrated pREP5cdc2 and cdc13 from episomal pREP41-cdc13. The nmt1 promoter is derepressed Figure 4 ------- COMMENT: 86f72e4a0d568a17 12 VKfLyWEObSbyLUogoEjpWLEjc1Q cdc10-129 cells blocked in G1 over expressing cdc2 from integrated pREP5cdc2 and cdc13 from episomal pREP41-cdc13. The nmt1 promoter is derepressed Figure 4 ------- COMMENT: 86f72e4a0d568a17 13 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 86f72e4a0d568a17 15 aD05ugqbDb473I14n+Qcq6QWv0s cdc13delete cells were kept alive by episomal pSM2 cdc13. Cell phenotype was observed after plasmid loss. Figure 2C ------- COMMENT: 86f72e4a0d568a17 16 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 86f9dd6401c5e32f 1 a1+byItgZTj3gKYhNrsDd0SMGNY When the branched actin nucleator Arp2/3 complex is inhibited, Pak1 stabilizes at a cell end, preventing localization of the GEF and Scd1, and disrupting the positive and negative feedback loops needed for periodic Cdc42 activity at that end. ------- COMMENT: 86f9dd6401c5e32f 4 CVkVKvsXHG8UAbnXos1ukcCDxw0 Fig. 7G ------- COMMENT: 86f9dd6401c5e32f 5 jAMzhtDCJtWQ8rgYQGX/0GfKI78 Fig. 7C and D ------- COMMENT: 86f9dd6401c5e32f 9 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 86f9dd6401c5e32f 10 QTnBz7fX8qu/SUPGfwkEyZ9lYGc Fig. S1A and B ------- COMMENT: 86f9dd6401c5e32f 11 qmvA+5SYIVf67cGxS+Xs4pnKS2o Fig. S1C and D ------- COMMENT: 86f9dd6401c5e32f 12 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 86f9dd6401c5e32f 13 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 86f9dd6401c5e32f 14 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 86f9dd6401c5e32f 15 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 86f9dd6401c5e32f 16 cqXE7Tt2P7hHYEEXckG/T8pYKeE The pak1-ts cells are characteristically mono- polar; however, we unexpectedly found that around 40% of these cells treated with CK-666 showed bipolar localization of Scd1-mNG (Fig. 5 A, asterisks). ------- COMMENT: 86f9dd6401c5e32f 17 qvLfj6uBlfZpMczhMXEF9D3liv8 Fig. 7A and B ------- COMMENT: 86f9dd6401c5e32f 18 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: 86f9dd6401c5e32f 19 vK/XEl5CoshbJfFXhN7pS9MQoGM Interestingly, we also find that Pak1 is required for normal endocytic patch dynamics. Fig. 9 ------- COMMENT: 8702be0070bbe887 2 oY8lGSOnUP3hzGS1yFY/Q7nshZk (Fig. 1A) ------- COMMENT: 8702be0070bbe887 3 oY8lGSOnUP3hzGS1yFY/Q7nshZk (Fig. 1A) ------- COMMENT: 8702be0070bbe887 4 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 8702be0070bbe887 5 Zi+v6L92VGscfcy0DWygtNIwIc8 Figure 3B indicates a bypass of cytokinesis checkpoint ------- COMMENT: 8702be0070bbe887 6 JGYyOc6NrKwNpeZZbXnjw99lpM4 Figure 3C (comment: CHECK shrunken cell) ------- COMMENT: 8702be0070bbe887 7 JGYyOc6NrKwNpeZZbXnjw99lpM4 Figure 3C (comment: CHECK shrunken cell) ------- COMMENT: 8702be0070bbe887 8 NkgiSoXnl2eU2ovsETC7Gc+HG6I (comment: CHECK allele esh2-1) ------- COMMENT: 8702be0070bbe887 9 G6i3RGudYTdmuHsSHF7YnLlGaOc Figure 4 GI Rho1 OEX rescues echinocandin sensitivity ------- COMMENT: 8702be0070bbe887 10 wZCpt3ogWqZrRsau9v1KCZUh1ZE figure4 ------- COMMENT: 8702be0070bbe887 11 fy0VyKx5akH5+sI1poX9cNIyFrE Figure 5 These results indicate that Rgf3p acts as a specific Rho1p activator in S. pombe. ------- COMMENT: 8702be0070bbe887 12 RZ3TOyr5VgKEQ1LlQNRKxe7XpbE Fig. 6A + DAPI staining revealed that, in most multiseptate cells, each compartment contained one nucleus, indicative of a defect in cell separation after septum assembly (not shown) ------- COMMENT: 8702be0070bbe887 13 IoakjHMl8a8N79rlq1sH5NgzHAs (Fig. 6B)....an increase in enzymatic activity was detected in cells overexpressing rgf3+ compared with the activity observed in the wild-type strain ------- COMMENT: 8702be0070bbe887 14 w7mM2RA6EHRmV3ykrXkJ/7A7k+A (1-3 beta D) As shown in Fig. 6C, there was an increase in the amount of β-glucan in cells that overexpressed rgf3+ compared with wild-type cells (16% and 10%, respectively), ------- COMMENT: 8702be0070bbe887 15 lPmiOafs7qAbrZr6WnWQEMSHP18 As shown in Fig. 6C, there was an increase in the amount of β-glucan in cells that overexpressed rgf3+ compared with wild-type cells (16% and 10%, respectively), ------- COMMENT: 8702be0070bbe887 17 oJHpZlkj82yR0draFwNGeYr9m1o (comment: CHECK replace with cytokinetic phase) ------- COMMENT: 87061c0c995c08bb 52 e7pEH9cwtM2aH/1ueSlhNpPoCoM (comment: also has AP1 binding site) ------- COMMENT: 8725231a57c6eeb3 6 nDRftXy0hwLokvGKvCLlnHuwHy0 cortical location/microtubules did not show a lateral interaction with the cell corte/Ninety-two percent of microtubules in num1D cells underwent catastrophe Figure 3.—Nuclear behavior in wild-type and num1D zy- gotes. Chromosomal DNA in zygotes (JY450 or JV627) was stained with Hoechst 33342 and monitored. The numbers on the left indicate time in minutes. Microtubules were visu- alized simultaneously by GFP-tagged a-tubulin. Stained DNA is shown in red, and GFP fluorescence in green. Bar, 5 mm. within 2 min of contacting the cell cortex (n 1⁄4 59). In contrast, 80% of microtubules that interacted with the cell cortex laterally in wild-type cells remained at the cell ends for .2 min (n 1⁄4 15). ------- COMMENT: 8725231a57c6eeb3 7 cHwi5OFv7+fxd40eZs/8MDaDXuQ (comment: CHECK membrane anchor) ------- COMMENT: 8742732cbb42bffd 1 GCJVELJKmCcJDAB10/EQ7G2M3Ac The increased proteasome ac- tivity correlates with the upregulation of the 20S CP assembly chaperone Ump1 and with an increase in proteasome assembly. Ump1 inactivation compromises 20S proteasome assembly, resulting in a drop in mature 26S/30S proteasomes. ------- COMMENT: 8742732cbb42bffd 3 p3x2dDTjQlS9jbO+IMHpHbED4nw (Fig. 1D) ------- COMMENT: 8742732cbb42bffd 4 SvlFFz9CaCJByQRBTLvdFnPqSYg (Fig. 1F) ------- COMMENT: 8742732cbb42bffd 5 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A and B) ------- COMMENT: 8742732cbb42bffd 8 lzDpBhI+e1KR8hAG7s00IgdEh2I (Fig. 5A) ------- COMMENT: 8742732cbb42bffd 10 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 8742732cbb42bffd 11 csvhQNV2cJjc7HiofC4evIQsxOM (Fig. 4C and Fig. S4A) ------- COMMENT: 8742732cbb42bffd 12 na8boxULcmrLrpxyNgxZlFRYT9g (Fig. S3B) ------- COMMENT: 8742732cbb42bffd 13 GkI93EYSqP6NybAtsJjxO2fy3Ic (Fig. 4D) ------- COMMENT: 8742732cbb42bffd 14 MfhXrratyk9HEpPvYNKyPqJLEoI (Fig. 4E) ------- COMMENT: 8742732cbb42bffd 15 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 8742732cbb42bffd 16 DES5Jkiy+rZ7wehRYgobQ94L1Q8 (Fig. 2G) ------- COMMENT: 8742732cbb42bffd 18 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: 8742732cbb42bffd 19 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: 8742732cbb42bffd 20 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: 8742732cbb42bffd 21 lzDpBhI+e1KR8hAG7s00IgdEh2I (Fig. 5A) ------- COMMENT: 8742732cbb42bffd 23 lzDpBhI+e1KR8hAG7s00IgdEh2I (Fig. 5A) ------- COMMENT: 8742732cbb42bffd 24 uytlu1SOwRPui227BsVN/IEzOy4 (Fig. S2A) ------- COMMENT: 8742732cbb42bffd 25 5CJGbfbqbHyxKnUlsBcTlwER03M (Fig. S2B and C) ------- COMMENT: 8742732cbb42bffd 27 5E/U+ckfaoKS/iVzTeQ1yIDhSN0 (Fig. S2G) ------- COMMENT: 8742732cbb42bffd 28 RD7246lbLWeEd/53Eqj3QTu+YBk (Fig. S3D) ------- COMMENT: 8742732cbb42bffd 29 RD7246lbLWeEd/53Eqj3QTu+YBk (Fig. S3D) ------- COMMENT: 8742732cbb42bffd 31 4CJ6vx8Kjku2u9dbK5ZoujERcjU (Fig. S3F) ------- COMMENT: 8742732cbb42bffd 32 UiG5r5ufQlxFfkfkP/H+hJFW9Sk (Fig. S3E) ------- COMMENT: 8742732cbb42bffd 33 UiG5r5ufQlxFfkfkP/H+hJFW9Sk (Fig. S3E) ------- COMMENT: 8742732cbb42bffd 34 bVkLbvkED/e2N/w+Sqbzfpr5oTc (Fig. S3G) ------- COMMENT: 8742732cbb42bffd 35 bVkLbvkED/e2N/w+Sqbzfpr5oTc (Fig. S3G) ------- COMMENT: 8742732cbb42bffd 36 AZkNyt6hRyyDl+KCz1eh2ak1x6g (Fig. S4A) ------- COMMENT: 8742732cbb42bffd 37 AZkNyt6hRyyDl+KCz1eh2ak1x6g (Fig. S4A) ------- COMMENT: 8742732cbb42bffd 38 W4xkP4H/sC1fm9WHOucBJfKfR7Y (Fig. 2) ------- COMMENT: 8742732cbb42bffd 39 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: 8742732cbb42bffd 40 lEBc1mIs7o84aIwMeFBPA4V17No (Fig. S3H) ------- COMMENT: 8742732cbb42bffd 41 lEBc1mIs7o84aIwMeFBPA4V17No (Fig. S3H) ------- COMMENT: 8742732cbb42bffd 42 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: 8742732cbb42bffd 43 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: 8742732cbb42bffd 44 gA4P48uKuk6ti5ukTRuiFvXo/Zw (Fig. S2C) ------- COMMENT: 8742732cbb42bffd 46 B+51PdZSwSquo7jRIKWKTvEByG4 (Fig. S2E) ------- COMMENT: 8742732cbb42bffd 47 AZkNyt6hRyyDl+KCz1eh2ak1x6g (Fig. S4A) ------- COMMENT: 8742732cbb42bffd 48 AZkNyt6hRyyDl+KCz1eh2ak1x6g (Fig. S4A) ------- COMMENT: 8742732cbb42bffd 50 NuNHLmtoq4GzF6sDw6D9LwteRCo (Fig. S4B) ------- COMMENT: 8742732cbb42bffd 51 NuNHLmtoq4GzF6sDw6D9LwteRCo (Fig. S4B) ------- COMMENT: 876a4a699dfe65d2 1 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: 876a4a699dfe65d2 4 wb8+3kAgYc7JGuHj/SRKrzp4b3M figb ------- COMMENT: 876a4a699dfe65d2 10 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 876a4a699dfe65d2 11 mvNOPGekPiUp9WnSf8gbLPEyYVY figa ------- COMMENT: 878405351e803458 4 9BSDRu77gCcpiKi803iu7V3zM+k table 2 ------- COMMENT: 878405351e803458 9 88B/FF9PvhJN9wjH6WXmnFBBq5Q figure 2a ------- COMMENT: 878405351e803458 10 88B/FF9PvhJN9wjH6WXmnFBBq5Q figure 2a ------- COMMENT: 878405351e803458 22 LGfpHLn9h4uWmR3sx2qpmMIutWw figure 5 b ------- COMMENT: 87c7e4f7306c2d8c 31 PAdW0rPzATDEKwybGU7BwkyjkKs (comment: inferred directness from effects of different alleles and of mutations elsewhere (swi1delta, clr4delta, or mat1-SS2)) ------- COMMENT: 87c7e4f7306c2d8c 32 PAdW0rPzATDEKwybGU7BwkyjkKs (comment: inferred directness from effects of different alleles and of mutations elsewhere (swi1delta, clr4delta, or mat1-SS2)) ------- COMMENT: 87c7e4f7306c2d8c 82 CFSaA8GNBbxtP3X77DUxzHcLQ/g (comment: inferred indirectness from author description and different effect of swi1delta) ------- COMMENT: 87c7e4f7306c2d8c 99 4GaS2e2iHc99kYj83FxAKApTVR0 (comment: same as lsd1-E918 single mutant) ------- COMMENT: 87c7e4f7306c2d8c 100 4GaS2e2iHc99kYj83FxAKApTVR0 (comment: same as lsd1-E918 single mutant) ------- COMMENT: 87c7e4f7306c2d8c 101 4GaS2e2iHc99kYj83FxAKApTVR0 (comment: same as lsd1-E918 single mutant) ------- COMMENT: 87c7e4f7306c2d8c 102 4GaS2e2iHc99kYj83FxAKApTVR0 (comment: same as lsd1-E918 single mutant) ------- COMMENT: 87c7e4f7306c2d8c 103 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 87f2700b47b2492e 6 jdATZwhdt5cEevWcMPKnhyHjASE (comment: says increased proportion, which is a synonym) ------- COMMENT: 87f689d02b0fb234 6 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 87f689d02b0fb234 7 4kVu9GcjvL+FA2tY0zalDwHR204 figure 3A/B ------- COMMENT: 87f689d02b0fb234 8 m7LnMUBifspGwe9xdjkomYoLWVc Figure 5B the iss1-DC mutation significantly reduced H3K9me2 at both ssm4 and mei4). ------- COMMENT: 87f689d02b0fb234 10 NCpVtxWVkVd/CC2TTkJAwIl3ZfA figure 3E ------- COMMENT: 87f689d02b0fb234 11 m9PuQZMN4JgcGJV1KB/6bsVsuIQ figure 3F ------- COMMENT: 87f689d02b0fb234 12 JXoxAqq/HDpJH7JuadLnmIVxSvQ Figure 4C; Figure S3 Although the percentage of total reads was relatively small, the iss1-DC mutation caused a reproducible and statistically sig- nificant extension of the 30 end of transcripts by about 200 nt. ------- COMMENT: 87f689d02b0fb234 14 yGZkJ5y+5u2XUNrmqh0yLeWB89o fig 4e The 73 genes with increased expression in the iss1-DC mutant were evaluated for common functions and were strongly enriched for factors important for iron assimilation GO:0033212. ------- COMMENT: 87f689d02b0fb234 15 6cGtC/+YDY6LhIbht5aEwH4xG3M fig 4g & S4. /Figure 5A) ------- COMMENT: 87f689d02b0fb234 16 6cGtC/+YDY6LhIbht5aEwH4xG3M fig 4g & S4. /Figure 5A) ------- COMMENT: 87f689d02b0fb234 17 m7LnMUBifspGwe9xdjkomYoLWVc Figure 5B the iss1-DC mutation significantly reduced H3K9me2 at both ssm4 and mei4). ------- COMMENT: 87f689d02b0fb234 19 IqOLX8J+ceKoCWW3ZqCa96S9Ogc Figures 5D–5G: Our coIP experiments revealed Iss1 interacts with Rrp6, Mmi1, and Pla1, indicating that Iss1 is associated with this network of elimination factors ------- COMMENT: 87f689d02b0fb234 20 IqOLX8J+ceKoCWW3ZqCa96S9Ogc Figures 5D–5G: Our coIP experiments revealed Iss1 interacts with Rrp6, Mmi1, and Pla1, indicating that Iss1 is associated with this network of elimination factors ------- COMMENT: 87f689d02b0fb234 21 IqOLX8J+ceKoCWW3ZqCa96S9Ogc Figures 5D–5G: Our coIP experiments revealed Iss1 interacts with Rrp6, Mmi1, and Pla1, indicating that Iss1 is associated with this network of elimination factors ------- COMMENT: 87f689d02b0fb234 22 YOIBVg8X4DEnnhbMkJ0pik0xV30 Figure 5F the iss1-DC truncation did disrupt its interaction with Mmi1. ------- COMMENT: 87f689d02b0fb234 23 gCg1fu2aXIq2QTjnD+O4EauhLbc Figure S5 However, the truncation did not reduce Iss1 interaction with Rrp6 ------- COMMENT: 87f689d02b0fb234 24 Lww8Fj6eGJj00KwUKWP+XojXsUg Figure 5H. We found that Iss1 assembles into nuclear dots, and these co-localized with Pla1, indicating that Iss1 also assembles in vivo with RNA elimination factors ------- COMMENT: 883442658ddcd9a9 1 9QYjTBqL6QTIBGzkALOzDzbwqoE Serine 481 is phosphorylated by Cig2/Cdc2 during meiosis I. Phosphorylation decreases Fkh2 DNA binding affinity ------- COMMENT: 883442658ddcd9a9 23 jNkvbZWqY9apSFavkRpgBmMC0w8 EMSA fig4 ------- COMMENT: 883442658ddcd9a9 31 jNkvbZWqY9apSFavkRpgBmMC0w8 EMSA fig4 ------- COMMENT: 884c35ae47e3fec8 1 ZfGypdrrv/TjcCOseWai+w8c0vM These phosphorylation sites were identified by the phos-tag analysis, phospho-specific antibodies, and in vitro phosphorylation assay ------- COMMENT: 884c35ae47e3fec8 2 hMi8I4gfEsr4Y0AWMJ2GiNqCi+s ------- COMMENT: 884c35ae47e3fec8 5 Oe7Rm1DjiE9i0AO246Kud6e5AVE This phenotype is observed in the presence of ICRF-193, a bisdioxopiperazine derivative [meso-4,4-(2,3-butanediyl)-bis (2,6-piperazinedione)], a catalytic topo II inhibitor. ------- COMMENT: 884c35ae47e3fec8 6 nEhfmFAag9kHy0l8exRMKU0maQ4 (comment: ICRF-193, a bisdioxopiperazine derivative [meso-4,4-(2,3-butanediyl)-bis (2,6-piperazinedione)], is a catalytic topo II inhibitor) ------- COMMENT: 884c35ae47e3fec8 8 hMi8I4gfEsr4Y0AWMJ2GiNqCi+s ------- COMMENT: 884c35ae47e3fec8 9 hMi8I4gfEsr4Y0AWMJ2GiNqCi+s ------- COMMENT: 884c35ae47e3fec8 10 hMi8I4gfEsr4Y0AWMJ2GiNqCi+s ------- COMMENT: 884c35ae47e3fec8 11 hMi8I4gfEsr4Y0AWMJ2GiNqCi+s ------- COMMENT: 884c35ae47e3fec8 12 hMi8I4gfEsr4Y0AWMJ2GiNqCi+s ------- COMMENT: 884c35ae47e3fec8 13 aNoUk0Tp4SAyvsePQJvOQiSLEIs (comment: also assayed directly using human CKII) ------- COMMENT: 885eefd237119afe 18 olZLboBjIw7ABIhPKmy3m8HNK08 (comment: normal with and without spindle checkpoint activation) ------- COMMENT: 885eefd237119afe 21 8Qt4lNnmUGv/1BBZGVbkQo+qpYI (comment: CHECK in vitro) ------- COMMENT: 885eefd237119afe 22 olZLboBjIw7ABIhPKmy3m8HNK08 (comment: normal with and without spindle checkpoint activation) ------- COMMENT: 885eefd237119afe 25 8Qt4lNnmUGv/1BBZGVbkQo+qpYI (comment: CHECK in vitro) ------- COMMENT: 885eefd237119afe 26 8Qt4lNnmUGv/1BBZGVbkQo+qpYI (comment: CHECK in vitro) ------- COMMENT: 885eefd237119afe 31 8Qt4lNnmUGv/1BBZGVbkQo+qpYI (comment: CHECK in vitro) ------- COMMENT: 885eefd237119afe 41 jJRiuPI9LuEVNUvuUosQYs5+fyY Hhp1 accumulates at SPB when spindle checkpoint activated ------- COMMENT: 885eefd237119afe 48 8Qt4lNnmUGv/1BBZGVbkQo+qpYI (comment: CHECK in vitro) ------- COMMENT: 885eefd237119afe 52 8Qt4lNnmUGv/1BBZGVbkQo+qpYI (comment: CHECK in vitro) ------- COMMENT: 885eefd237119afe 78 8Qt4lNnmUGv/1BBZGVbkQo+qpYI (comment: CHECK in vitro) ------- COMMENT: 885eefd237119afe 79 8Qt4lNnmUGv/1BBZGVbkQo+qpYI (comment: CHECK in vitro) ------- COMMENT: 886d6f892477dd42 1 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: 886d6f892477dd42 3 I9vbDpNSv0iNjoZRYtmRou259sU We confirmed it by microscopy as well. Fig 3A and B. Deletion of doa10+ partially restored GFP–Bqt4 levels in the absence of Bqt3, whereas deletion of hrd1+ did not (Fig. 3A,B, comparing hrd1Δ to doa10Δ) ------- COMMENT: 886d6f892477dd42 4 UD2O2HhDWM4S6L8B7m6wW5ZtAAg We confirmed it by microscopy as well. Fig 3C and D. We also measured the amount of GFP–Bqt4 in mutants lacking Ubc6 and Ubc7, which are E2 ubiquitin- conjugating enzymes associated with Doa10 (Swanson et al., 2001). We found that GFP–Bqt4 levels increased to some extent in ubc6Δ and ubc7Δ single and ubc6Δ ubc7Δ double mutants (Fig. 3C,D). ------- COMMENT: 886d6f892477dd42 5 UD2O2HhDWM4S6L8B7m6wW5ZtAAg We confirmed it by microscopy as well. Fig 3C and D. We also measured the amount of GFP–Bqt4 in mutants lacking Ubc6 and Ubc7, which are E2 ubiquitin- conjugating enzymes associated with Doa10 (Swanson et al., 2001). We found that GFP–Bqt4 levels increased to some extent in ubc6Δ and ubc7Δ single and ubc6Δ ubc7Δ double mutants (Fig. 3C,D). ------- COMMENT: 886d6f892477dd42 6 IjSALdczVY/wzRYo5NYTM8BALtU Fig5C and D ------- COMMENT: 886d6f892477dd42 7 8XcY9/O98BDEJ6FveF1jvF46iLA We confirmed it by microscopy as well. Fig 2A and B. We found that GFP–Bqt4 degradation was suppressed in cut8-563 cells shifted to the nonpermissive temperature of 36°C (Fig. 2A,B). ------- COMMENT: 886d6f892477dd42 8 UMLjWSmZt6FaPWhzQFge3C/ejRg We confirmed it by microscopy as well. Fig 1C and D. ------- COMMENT: 886d6f892477dd42 9 M4LomI/tSsWZzCzXTfSxGVaX5s4 Fig S1. ------- COMMENT: 886d6f892477dd42 10 JdwO+d2W5mFifm9/jyMjQuAFebo GFP–Bqt4 fluorescence in the nuclei of both bqt3+ and bqt3Δ cells was elevated (Fig. 1A) ------- COMMENT: 886d6f892477dd42 11 0RX7oFbiD/We/tnFoUUgbhsJUDI To confirm this result, we measured protein levels by western blotting, which consistently showed an increase in GFP–Bqt4 protein levels in these strains upon proteasomal inhibition by BZ Fig. 1B ------- COMMENT: 886d6f892477dd42 12 o4Zn/p5W7v/5j5bm8HDaUQlAdkc Ubiquitinated forms of Bqt4 were detected in bqt3Δ cells and were enriched in both bqt3+ and bqt3Δ cells when proteasomal activity was inhibited (Fig. 1E), suggesting that Bqt4 was targeted to the proteasome by polyubiquitin modification. ------- COMMENT: 886d6f892477dd42 13 o4Zn/p5W7v/5j5bm8HDaUQlAdkc Ubiquitinated forms of Bqt4 were detected in bqt3Δ cells and were enriched in both bqt3+ and bqt3Δ cells when proteasomal activity was inhibited (Fig. 1E), suggesting that Bqt4 was targeted to the proteasome by polyubiquitin modification. ------- COMMENT: 886d6f892477dd42 17 HpgjyQ6q/lz3st7Ov6fsQOEETeI We constructed mutants of E3 ligases and their components that have been suggested to localize to or function in the ER and nucleus, namely ......... The mutants tested showed no detectable increase in fluorescence, except for the hul5Δ mutant (Fig. S2) ------- COMMENT: 886d6f892477dd42 18 HpgjyQ6q/lz3st7Ov6fsQOEETeI We constructed mutants of E3 ligases and their components that have been suggested to localize to or function in the ER and nucleus, namely ......... The mutants tested showed no detectable increase in fluorescence, except for the hul5Δ mutant (Fig. S2) ------- COMMENT: 886d6f892477dd42 19 HpgjyQ6q/lz3st7Ov6fsQOEETeI We constructed mutants of E3 ligases and their components that have been suggested to localize to or function in the ER and nucleus, namely ......... The mutants tested showed no detectable increase in fluorescence, except for the hul5Δ mutant (Fig. S2) ------- COMMENT: 886d6f892477dd42 20 HpgjyQ6q/lz3st7Ov6fsQOEETeI We constructed mutants of E3 ligases and their components that have been suggested to localize to or function in the ER and nucleus, namely ......... The mutants tested showed no detectable increase in fluorescence, except for the hul5Δ mutant (Fig. S2) ------- COMMENT: 886d6f892477dd42 21 HpgjyQ6q/lz3st7Ov6fsQOEETeI We constructed mutants of E3 ligases and their components that have been suggested to localize to or function in the ER and nucleus, namely ......... The mutants tested showed no detectable increase in fluorescence, except for the hul5Δ mutant (Fig. S2) ------- COMMENT: 886d6f892477dd42 22 HpgjyQ6q/lz3st7Ov6fsQOEETeI We constructed mutants of E3 ligases and their components that have been suggested to localize to or function in the ER and nucleus, namely ......... The mutants tested showed no detectable increase in fluorescence, except for the hul5Δ mutant (Fig. S2) ------- COMMENT: 886d6f892477dd42 23 HpgjyQ6q/lz3st7Ov6fsQOEETeI We constructed mutants of E3 ligases and their components that have been suggested to localize to or function in the ER and nucleus, namely ......... The mutants tested showed no detectable increase in fluorescence, except for the hul5Δ mutant (Fig. S2) ------- COMMENT: 886d6f892477dd42 24 62q83LMsuEanDxiICfiTUzqC4Uo (comment: Bqt4 is an integral membrane protein) ------- COMMENT: 886d6f892477dd42 26 7yM0y2RwNnOCQSE3+rsHIwaliH8 The Bqt4 fragment containing the helix domain and the adjacent intrinsically disordered sequence (Bqt4C369–432) reproduced the behavior of full-length Bqt4 (Fig. 1A), that is, localization to the NE and responses to BZ, both in the presence and absence of Bqt3 (Fig. 6A, Bqt4C369–432). ------- COMMENT: 886d6f892477dd42 27 7yM0y2RwNnOCQSE3+rsHIwaliH8 The Bqt4 fragment containing the helix domain and the adjacent intrinsically disordered sequence (Bqt4C369–432) reproduced the behavior of full-length Bqt4 (Fig. 1A), that is, localization to the NE and responses to BZ, both in the presence and absence of Bqt3 (Fig. 6A, Bqt4C369–432). ------- COMMENT: 886d6f892477dd42 28 fR+GRyu98IdjaU74OAizhZmHesY The helix domain alone (Bqt4C394–432) showed weaker localization to the NE and was somewhat diffused to the membrane compartments in the cytoplasm; (Fig. 6A, Bqt4C394–432) ------- COMMENT: 886d6f892477dd42 29 2/pkI4qEviTmWGZXD6J+ym8tq90 nevertheless, this fragment was degraded in the absence of Bqt3 and its levels increased with BZ treatment (Fig. 6A, Bqt4C394–432) These results indicate that the C-terminal fragment containing residues 369–432 was necessary and sufficient for the NE localization of Bqt4, and its truncation successively reduced NE localization. These results also indicate that the C-terminal TMD of Bqt4 is sufficient for its proteasome-mediated degradation. ------- COMMENT: 886d6f892477dd42 30 JCojVCdZA1syG5KFRopwWfSUuFw The TMD alone (Bqt4C414–432) showed even weaker localization at the NE with diffusion to the cytoplasm, but showed the same responses, that is, degradation in the absence of Bqt3 and increased levels upon BZ treatment (Fig. 6A, Bqt4C414–432). ------- COMMENT: 886d6f892477dd42 31 JCojVCdZA1syG5KFRopwWfSUuFw The TMD alone (Bqt4C414–432) showed even weaker localization at the NE with diffusion to the cytoplasm, but showed the same responses, that is, degradation in the absence of Bqt3 and increased levels upon BZ treatment (Fig. 6A, Bqt4C414–432). ------- COMMENT: 886d6f892477dd42 32 pz59fOp3EzaOtnSq9KZ4qMZdGkk The fragment Bqt4C369–425 lost its dependency on Bqt3 for degradation and exhibited similar behaviors in the presence or absence of Bqt3 (Fig. 6A, Bqt4C369–425): ------- COMMENT: 886d6f892477dd42 33 pz59fOp3EzaOtnSq9KZ4qMZdGkk The fragment Bqt4C369–425 lost its dependency on Bqt3 for degradation and exhibited similar behaviors in the presence or absence of Bqt3 (Fig. 6A, Bqt4C369–425): ------- COMMENT: 886d6f892477dd42 34 wfdEeMBa/h2iAjx5Uo8UD2IE2ZQ The results of the yeast-two-hybrid assay showed that the fragments lacking the last seven residues did not bind to Bqt3 (Fig. 6B). ------- COMMENT: 886d6f892477dd42 35 1NNO28kba1djTh81pKYNIP+OEZw To ascertain whether this abnormal phenotype was caused by an accumulation of Bqt4, we overexpressed GFP–Bqt4 under the nmt1 promoter using the chemical compound YAM2 to control the expression level. YAM2 suppresses nmt1 promoter activity depending on its concentration (Nakamura et al., 2011). Overexpression of GFP–Bqt4 reproduced the nuclear- deformed morphology (Fig. 7B), ------- COMMENT: 886d6f892477dd42 36 JbP0vJO3OFuFm+9E2QwX182NG1c These results are consistent with previous studies that showed that Doa10 partially localizes to the INM and is involved in the degradation of certain nuclear and INM substrates, whereas Hrd1 is found exclusively in the ER (Deng and Hochstrasser, 2006; Boban et al., 2014). ------- COMMENT: 888814a3416fe952 1 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: 888814a3416fe952 2 QxyA7Kxt4u0SZftovXPYBddoxhU Fig 1D ------- COMMENT: 888814a3416fe952 3 jgoGuBgb+Ot76esr6oM9CSFJl3I Fig 1E ------- COMMENT: 888814a3416fe952 4 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: 888814a3416fe952 5 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: 888814a3416fe952 6 QxyA7Kxt4u0SZftovXPYBddoxhU Fig 1D ------- COMMENT: 888814a3416fe952 7 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: 888814a3416fe952 8 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: 888814a3416fe952 9 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: 888814a3416fe952 10 cWU1gxrBhSdpW25jqWFTdTZ9qBA fig 3A ------- COMMENT: 888814a3416fe952 11 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: 888814a3416fe952 12 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: 888814a3416fe952 13 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: 888814a3416fe952 14 1DSx+sDsm3xYDLtMfzxH/90R880 Fig. 3C. ------- COMMENT: 888814a3416fe952 15 1DSx+sDsm3xYDLtMfzxH/90R880 Fig. 3C. ------- COMMENT: 888814a3416fe952 16 t6uyTtt4P/8kRlIjC2qelSNbzfs Fig 3C ------- COMMENT: 888814a3416fe952 17 t6uyTtt4P/8kRlIjC2qelSNbzfs Fig 3C ------- COMMENT: 888814a3416fe952 18 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 888814a3416fe952 19 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: 888814a3416fe952 20 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: 888814a3416fe952 21 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: 888814a3416fe952 22 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: 888814a3416fe952 24 1DSx+sDsm3xYDLtMfzxH/90R880 Fig. 3C. ------- COMMENT: 888814a3416fe952 25 1DSx+sDsm3xYDLtMfzxH/90R880 Fig. 3C. ------- COMMENT: 888814a3416fe952 26 zX8cnyVWPKQU6GmVTQcqhfULA0o Fig 5DE ------- COMMENT: 888814a3416fe952 28 cZv/LZWPjqm0GRAJq9Q1fBmcJ0o Supp: S1A ------- COMMENT: 888814a3416fe952 29 iR65dJNlxazjxQ7EePXf/0HAddk Supp: S1B ------- COMMENT: 888814a3416fe952 30 4hxGT7pLh8CfRDWZXwMEvfPcDPM Fig. S1C ------- COMMENT: 888814a3416fe952 31 4hxGT7pLh8CfRDWZXwMEvfPcDPM Fig. S1C ------- COMMENT: 889ae07851beec32 1 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 889ae07851beec32 2 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 889ae07851beec32 3 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 889ae07851beec32 4 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 889ae07851beec32 5 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 889ae07851beec32 6 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 889ae07851beec32 7 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 889ae07851beec32 8 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 889ae07851beec32 9 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 889ae07851beec32 10 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 889ae07851beec32 11 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 889ae07851beec32 14 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 889ae07851beec32 15 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 889ae07851beec32 16 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 889ae07851beec32 17 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 889ae07851beec32 18 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 889ae07851beec32 19 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 889ae07851beec32 20 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 889ae07851beec32 21 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 889ae07851beec32 22 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C,D ------- COMMENT: 889ae07851beec32 23 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C,D ------- COMMENT: 889ae07851beec32 24 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C,D ------- COMMENT: 889ae07851beec32 25 i7zdy0Zq11VC88wcz9jFrMoWmKo We conclude that fission yeast cytokinesis uses two over- lapping mechanisms to position Mid1 at the central cortex. First, Cdr2 anchors Mid1 at the medial cortex during interphase through a physical interaction. ------- COMMENT: 889ae07851beec32 26 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 889b897cf264bfe3 1 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 889b897cf264bfe3 2 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 889b897cf264bfe3 3 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 889b897cf264bfe3 5 33liLed3vbaDqIxtg+l7aKZrZ/w fig2 lane 11-12 ------- COMMENT: 889b897cf264bfe3 6 /fmAK5m1BOv744L376+H/Db/gJA fig2,5 ------- COMMENT: 889b897cf264bfe3 7 bEkfsF17u46pwPhPfGB1qqF946Q fig2,3,4 ------- COMMENT: 88a0961bc71fcefc 1 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 88a0961bc71fcefc 2 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 88a0961bc71fcefc 3 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 88a0961bc71fcefc 4 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 88a0961bc71fcefc 5 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 88a0961bc71fcefc 6 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 88a0961bc71fcefc 7 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 88a0961bc71fcefc 8 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 88a0961bc71fcefc 9 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 88a0961bc71fcefc 10 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 88a0961bc71fcefc 11 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 88a0961bc71fcefc 12 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 88a0961bc71fcefc 13 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 88a0961bc71fcefc 14 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 88a0961bc71fcefc 15 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 88d2e1acdc9993bc 2 1oHM4plJlukbgINk0PhiwCs1+yU Figure 2a (comment: Boundary of non growing cell end maintained) ------- COMMENT: 88d2e1acdc9993bc 3 F1Eicu0DEeQ5zwpXxZjGyPcZcwE Figure 2b (comment: Boundary of non growing cell end maintained) ------- COMMENT: 88d2e1acdc9993bc 4 xMqsLYymIPpTICeuk6VlML3UDmc Figure 2c (comment: Boundary of the non growing cell end not maintained) ------- COMMENT: 88d2e1acdc9993bc 5 BoiUajKU22FNSUGh76OS2OSFZ8Q Figure 3 (comment: F actin is absent from non growing end) ------- COMMENT: 88d2e1acdc9993bc 6 kMIQbKkt2b1/jTIM2ff0RpTfzME Figure 4 (comment: Ral3/cor-CGFP fusion is expressed from pMral3/cor-C) ------- COMMENT: 88d2e1acdc9993bc 7 /pR4s11rtq7Yj5/EplEepsmJsRk Figure 4 (comment: Ral3/cor-CGFP fusion is expressed from pMral3/cor-C) ------- COMMENT: 88d2e1acdc9993bc 8 /pR4s11rtq7Yj5/EplEepsmJsRk Figure 4 (comment: Ral3/cor-CGFP fusion is expressed from pMral3/cor-C) ------- COMMENT: 88d2e1acdc9993bc 9 4nrR2C+ZjhJhAt/N6t/sOn/8oTQ (comment: vw: jacky suggested "protein localisation to the lateral plasma membrane" but will keep as parent Figures 1, S1 and S2. Cor-C GFP is probably episomal but it is not clear) ------- COMMENT: 88d2e1acdc9993bc 10 DFpJ+TbG6nhqe6+WCJoi0TRg84I Figure 3 (comment: normal at non-growing end) ------- COMMENT: 88d2e1acdc9993bc 11 DFpJ+TbG6nhqe6+WCJoi0TRg84I Figure 3 (comment: normal at non-growing end) ------- COMMENT: 88f3e63970618c74 1 xBaYcsRhwnmYXuj504BDOLRygP4 In contrast, the monomeric Mid13A remained concentrated in the nucleus and its signals on the plasma membrane were more widespread, even reached the cell poles (Figure 6E ------- COMMENT: 88f3e63970618c74 4 6DRxb39Eecyakwvox/mq9fam9cc Interestingly, it binds to PI(4,5)P2 strongly, with a Kd up to 0.12 μM (Figure 3C, 3D). ------- COMMENT: 88f3e63970618c74 5 xZkwg8xqrXqJZBEnXyhCER/Q79I Mutations of the hydrophobic C2-C2 interface shifted Mid1 into monomeric state (Figure 6A), ------- COMMENT: 88f3e63970618c74 6 DDbG/bmGetrETmFYOvwMxrFbKX4 which showed > 10-fold lower affinity to PI(4,5)P2 (Figure 3D). ------- COMMENT: 88f3e63970618c74 7 WjTpgU9BgJpGZSTGMG3bvtGc/n4 In contrast, monomerization only slightly reduced the affinity to PS. ------- COMMENT: 88f3e63970618c74 8 d5aMji3xsTCq0QfYCwXzMdhCIRc Indeed, the morphology and positioning of the contractile ring marked with myosin regulatory light chain Rlc1 were normal in mid13A cells (Figure S6, C-D) ------- COMMENT: 88f3e63970618c74 9 wB3LMa9aOXWmTmC2Qtiz1wBYJfY However, contractile ring assembly was significantly faster in mid13A than in mid1+ cells (Figure S6, C–F), ------- COMMENT: 88f3e63970618c74 10 SnGGyuCxi7peJFUAuS+J2b41eYg which interacts with the N-terminus of Mid1 and stabilizes Mid1 at the division plane (Ye et al., 2012; Zhu et al., 2013). As expected, mid13A gef2Δ double mutants had strong synthetic defects in division-plane placement and septum formation at 36°C (Figure 6, F–H). ------- COMMENT: 88f605195653964e 6 TCgWy6SfOISByfzS9E8DJuhfKmI transcription run-on assay ------- COMMENT: 88f605195653964e 9 UCJP9HKC6RZgy7vWNweyvIFEXgc steady-state labeling assay; stability increases in wt but not mutant upon UV exposure ------- COMMENT: 88f605195653964e 13 TCgWy6SfOISByfzS9E8DJuhfKmI transcription run-on assay ------- COMMENT: 88f605195653964e 14 Y/6U3jZJRq2yCC0E4JGAHBZbpYE steady-state labeling assay; stability increases in wt but not sty1delta upon UV exposure ------- COMMENT: 88f8cd06ecf00d84 1 jZSxJjgagcL8b1t+2EHOQsTdLWg present throughout cell cycle but at lower level in S phase ------- COMMENT: 88f8cd06ecf00d84 2 JcUv06Cj8MJroMJTlXgkP/KvGck present throughout cell cycle but at higher level in S phase ------- COMMENT: 88f8cd06ecf00d84 3 PJ46GceEjIBrhcjfyCzFteoSH5s DNA polymerases present in late S; epsilon (cdc20) earlier than alpha (pol1) or delta (cdc6) ------- COMMENT: 88f8cd06ecf00d84 4 B99tjjPcTFkdCFBVBxDjhqo+5bw present in late S ------- COMMENT: 88f8cd06ecf00d84 5 3sFyMgQdrko3inbbEqHI7CGLmTU present at roughly constant level throughout cell cycle ------- COMMENT: 88f8cd06ecf00d84 6 PJ46GceEjIBrhcjfyCzFteoSH5s DNA polymerases present in late S; epsilon (cdc20) earlier than alpha (pol1) or delta (cdc6) ------- COMMENT: 88f8cd06ecf00d84 7 WxwjEZ/m+qHmsoS5AC2vdcAFEAA present in late S, as late as pols alpha & delta ------- COMMENT: 88f8cd06ecf00d84 8 PJ46GceEjIBrhcjfyCzFteoSH5s DNA polymerases present in late S; epsilon (cdc20) earlier than alpha (pol1) or delta (cdc6) ------- COMMENT: 88f8cd06ecf00d84 9 B99tjjPcTFkdCFBVBxDjhqo+5bw present in late S ------- COMMENT: 88f8cd06ecf00d84 10 JcUv06Cj8MJroMJTlXgkP/KvGck present throughout cell cycle but at higher level in S phase ------- COMMENT: 88f8cd06ecf00d84 11 B99tjjPcTFkdCFBVBxDjhqo+5bw present in late S ------- COMMENT: 88f8cd06ecf00d84 12 +S/6Y3uyJvJuN7OLTSn9WXGvLzc present throughout cell cycle but at higher level in late S phase ------- COMMENT: 89027fe6a1134146 1 zlVZSciQhkT3ddCUv7WQzTTKgUw a reminder why we compounded these phenotypes: nuclear accumulation of poly(A)+ RNA was observed only in cells with the cut phenotype in ptr11-1. No nuclear accumulation was observed in cells without the cut phenotype indicating a possible relationship between the cut phenotype and the nuclear accumulation of poly(A)+ RNA in this mutant. ------- COMMENT: 89027fe6a1134146 3 zlVZSciQhkT3ddCUv7WQzTTKgUw a reminder why we compounded these phenotypes: nuclear accumulation of poly(A)+ RNA was observed only in cells with the cut phenotype in ptr11-1. No nuclear accumulation was observed in cells without the cut phenotype indicating a possible relationship between the cut phenotype and the nuclear accumulation of poly(A)+ RNA in this mutant. ------- COMMENT: 89027fe6a1134146 4 zlVZSciQhkT3ddCUv7WQzTTKgUw a reminder why we compounded these phenotypes: nuclear accumulation of poly(A)+ RNA was observed only in cells with the cut phenotype in ptr11-1. No nuclear accumulation was observed in cells without the cut phenotype indicating a possible relationship between the cut phenotype and the nuclear accumulation of poly(A)+ RNA in this mutant. ------- COMMENT: 89027fe6a1134146 5 zlVZSciQhkT3ddCUv7WQzTTKgUw a reminder why we compounded these phenotypes: nuclear accumulation of poly(A)+ RNA was observed only in cells with the cut phenotype in ptr11-1. No nuclear accumulation was observed in cells without the cut phenotype indicating a possible relationship between the cut phenotype and the nuclear accumulation of poly(A)+ RNA in this mutant. ------- COMMENT: 89027fe6a1134146 6 zlVZSciQhkT3ddCUv7WQzTTKgUw a reminder why we compounded these phenotypes: nuclear accumulation of poly(A)+ RNA was observed only in cells with the cut phenotype in ptr11-1. No nuclear accumulation was observed in cells without the cut phenotype indicating a possible relationship between the cut phenotype and the nuclear accumulation of poly(A)+ RNA in this mutant. ------- COMMENT: 89027fe6a1134146 7 zlVZSciQhkT3ddCUv7WQzTTKgUw a reminder why we compounded these phenotypes: nuclear accumulation of poly(A)+ RNA was observed only in cells with the cut phenotype in ptr11-1. No nuclear accumulation was observed in cells without the cut phenotype indicating a possible relationship between the cut phenotype and the nuclear accumulation of poly(A)+ RNA in this mutant. ------- COMMENT: 89027fe6a1134146 8 zlVZSciQhkT3ddCUv7WQzTTKgUw a reminder why we compounded these phenotypes: nuclear accumulation of poly(A)+ RNA was observed only in cells with the cut phenotype in ptr11-1. No nuclear accumulation was observed in cells without the cut phenotype indicating a possible relationship between the cut phenotype and the nuclear accumulation of poly(A)+ RNA in this mutant. ------- COMMENT: 89027fe6a1134146 9 zlVZSciQhkT3ddCUv7WQzTTKgUw a reminder why we compounded these phenotypes: nuclear accumulation of poly(A)+ RNA was observed only in cells with the cut phenotype in ptr11-1. No nuclear accumulation was observed in cells without the cut phenotype indicating a possible relationship between the cut phenotype and the nuclear accumulation of poly(A)+ RNA in this mutant. ------- COMMENT: 89027fe6a1134146 10 zlVZSciQhkT3ddCUv7WQzTTKgUw a reminder why we compounded these phenotypes: nuclear accumulation of poly(A)+ RNA was observed only in cells with the cut phenotype in ptr11-1. No nuclear accumulation was observed in cells without the cut phenotype indicating a possible relationship between the cut phenotype and the nuclear accumulation of poly(A)+ RNA in this mutant. ------- COMMENT: 89027fe6a1134146 11 zlVZSciQhkT3ddCUv7WQzTTKgUw a reminder why we compounded these phenotypes: nuclear accumulation of poly(A)+ RNA was observed only in cells with the cut phenotype in ptr11-1. No nuclear accumulation was observed in cells without the cut phenotype indicating a possible relationship between the cut phenotype and the nuclear accumulation of poly(A)+ RNA in this mutant. ------- COMMENT: 89027fe6a1134146 12 zlVZSciQhkT3ddCUv7WQzTTKgUw a reminder why we compounded these phenotypes: nuclear accumulation of poly(A)+ RNA was observed only in cells with the cut phenotype in ptr11-1. No nuclear accumulation was observed in cells without the cut phenotype indicating a possible relationship between the cut phenotype and the nuclear accumulation of poly(A)+ RNA in this mutant. ------- COMMENT: 89027fe6a1134146 13 zlVZSciQhkT3ddCUv7WQzTTKgUw a reminder why we compounded these phenotypes: nuclear accumulation of poly(A)+ RNA was observed only in cells with the cut phenotype in ptr11-1. No nuclear accumulation was observed in cells without the cut phenotype indicating a possible relationship between the cut phenotype and the nuclear accumulation of poly(A)+ RNA in this mutant. ------- COMMENT: 89027fe6a1134146 16 zlVZSciQhkT3ddCUv7WQzTTKgUw a reminder why we compounded these phenotypes: nuclear accumulation of poly(A)+ RNA was observed only in cells with the cut phenotype in ptr11-1. No nuclear accumulation was observed in cells without the cut phenotype indicating a possible relationship between the cut phenotype and the nuclear accumulation of poly(A)+ RNA in this mutant. ------- COMMENT: 89027fe6a1134146 17 zlVZSciQhkT3ddCUv7WQzTTKgUw a reminder why we compounded these phenotypes: nuclear accumulation of poly(A)+ RNA was observed only in cells with the cut phenotype in ptr11-1. No nuclear accumulation was observed in cells without the cut phenotype indicating a possible relationship between the cut phenotype and the nuclear accumulation of poly(A)+ RNA in this mutant. ------- COMMENT: 89027fe6a1134146 18 zlVZSciQhkT3ddCUv7WQzTTKgUw a reminder why we compounded these phenotypes: nuclear accumulation of poly(A)+ RNA was observed only in cells with the cut phenotype in ptr11-1. No nuclear accumulation was observed in cells without the cut phenotype indicating a possible relationship between the cut phenotype and the nuclear accumulation of poly(A)+ RNA in this mutant. ------- COMMENT: 89027fe6a1134146 20 zlVZSciQhkT3ddCUv7WQzTTKgUw a reminder why we compounded these phenotypes: nuclear accumulation of poly(A)+ RNA was observed only in cells with the cut phenotype in ptr11-1. No nuclear accumulation was observed in cells without the cut phenotype indicating a possible relationship between the cut phenotype and the nuclear accumulation of poly(A)+ RNA in this mutant. ------- COMMENT: 891bbad03583f932 1 ymtvGXDLG+HOoA8acYBzSfP7c1o (comment: (Sub lethal and lethat doese)) Figure 5 and Figure S3. Also in the Table 3. ------- COMMENT: 891bbad03583f932 2 Nj2P2uLJ/oGsKYOyTwbBIBduiUk Figure 3A and Figure S1A. Table 3. (comment: CHECK Suppression of the lytic phenotype at cytokinesis) ------- COMMENT: 891bbad03583f932 3 JkKCFAknZ9okDhxOkBC7baanIcU Figure 3B and Figure S1B. Also in the Table 3. Cytokinesis is blocked in both wild-type and pbr1-8 strains treated with lethal concentrations of the echinocandin drug anidulafungin, suggesting that this drug affects the function of Bgs4 and Bgs1 and/or Bgs3 ------- COMMENT: 891bbad03583f932 4 deB90ruMn+LGJQnIejIC02KBZM8 Figure 4A and Figure S2A (comment: Sublethal concentrations of caspofungin) ------- COMMENT: 891bbad03583f932 5 B2EADsCxlI13U836v2KwUytIQt4 Table 5 and Figure S3, and Figure S4C. Vegetative cell lysis caused by lethal and sublethal concentrations of micafungin is suppressed in the pbr1-8 ------- COMMENT: 891bbad03583f932 6 ozu4D7c/7bKkRTPPPKlnIjak5Rs Figure 4B and Figure S2B. Also in the Table 3. The pbr1-8 mutation partially suppresses the slowing cytokinesis caused by lethal concentrations of caspofungin, suggesting that besides Bgs4, this drug affects other Bgs subunits (Bgs1 and/or Bgs3) ------- COMMENT: 891bbad03583f932 10 deB90ruMn+LGJQnIejIC02KBZM8 Figure 4A and Figure S2A (comment: Sublethal concentrations of caspofungin) ------- COMMENT: 891bbad03583f932 11 ymtvGXDLG+HOoA8acYBzSfP7c1o (comment: (Sub lethal and lethat doese)) Figure 5 and Figure S3. Also in the Table 3. ------- COMMENT: 891bbad03583f932 12 B2EADsCxlI13U836v2KwUytIQt4 Table 5 and Figure S3, and Figure S4C. Vegetative cell lysis caused by lethal and sublethal concentrations of micafungin is suppressed in the pbr1-8 ------- COMMENT: 891bbad03583f932 13 ozu4D7c/7bKkRTPPPKlnIjak5Rs Figure 4B and Figure S2B. Also in the Table 3. The pbr1-8 mutation partially suppresses the slowing cytokinesis caused by lethal concentrations of caspofungin, suggesting that besides Bgs4, this drug affects other Bgs subunits (Bgs1 and/or Bgs3) ------- COMMENT: 89222eafc99f3372 26 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: 89222eafc99f3372 30 RALdV5r7BhvDDbTt+je/1Dr+PQA Fig. S1b ------- COMMENT: 89222eafc99f3372 33 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 89222eafc99f3372 34 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig. 2 ------- COMMENT: 89222eafc99f3372 36 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 89222eafc99f3372 37 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 89222eafc99f3372 38 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 89222eafc99f3372 39 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 89222eafc99f3372 42 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 89222eafc99f3372 44 7nkFkejREgDThj8O5WegYsaDowo Fig. 4G ------- COMMENT: 89222eafc99f3372 45 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 89222eafc99f3372 46 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 89222eafc99f3372 47 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 89222eafc99f3372 48 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 89222eafc99f3372 49 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 89222eafc99f3372 50 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 89222eafc99f3372 51 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 89222eafc99f3372 54 wd/ZbuqMwrYnhSdbyjisJhJW4ng Fig. 6 ------- COMMENT: 893c5c2ae65e3990 3 24+aUF1s7n3/gp5Nva1ebm2FBjg Figure 3B, Figure 3C ------- COMMENT: 893c5c2ae65e3990 4 kNh09J9Cz0Y2Km2wt9hyqVs4f/A Figure S15 ------- COMMENT: 893c5c2ae65e3990 10 eXWPNIslufPlAy5Ve5KCTnJvMXM Figure S2A ------- COMMENT: 893c5c2ae65e3990 11 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: 893c5c2ae65e3990 12 wf185u4DRDkg7Sbpsn3gLe6+2NY Figure 4E ------- COMMENT: 893c5c2ae65e3990 13 wf185u4DRDkg7Sbpsn3gLe6+2NY Figure 4E ------- COMMENT: 893c5c2ae65e3990 14 pYgX/kMr2UIygvYA5v5k2C6KhGw (comment: CHECK in vitro) Figure S1A, right pane ------- COMMENT: 893c5c2ae65e3990 15 pYgX/kMr2UIygvYA5v5k2C6KhGw (comment: CHECK in vitro) Figure S1A, right pane ------- COMMENT: 893c5c2ae65e3990 16 oJN940NlrCwYqfXpg4AMW5yPjdw figure 3C ------- COMMENT: 893c5c2ae65e3990 17 oJN940NlrCwYqfXpg4AMW5yPjdw figure 3C ------- COMMENT: 893c5c2ae65e3990 18 2k8cM50dNlhIwNL+99X8GPaiIAA figure 3C (comment: the N erminal domain has a dominent -ve effect in in vitro assay (not expression should ne n/a)) ------- COMMENT: 893c5c2ae65e3990 20 yoPV7T3XPl9iHWtD8GwLOeE2T4s Figure 4A) ------- COMMENT: 8945fdb7cb39b74b 1 5mX1z4CHzF6vU50VoKDjsGG7TFI (comment: assayed using 160-bp palindromic sequence inserted into ade6 locus) ------- COMMENT: 8945fdb7cb39b74b 2 5mX1z4CHzF6vU50VoKDjsGG7TFI (comment: assayed using 160-bp palindromic sequence inserted into ade6 locus) ------- COMMENT: 8945fdb7cb39b74b 3 5mX1z4CHzF6vU50VoKDjsGG7TFI (comment: assayed using 160-bp palindromic sequence inserted into ade6 locus) ------- COMMENT: 8945fdb7cb39b74b 4 5mX1z4CHzF6vU50VoKDjsGG7TFI (comment: assayed using 160-bp palindromic sequence inserted into ade6 locus) ------- COMMENT: 89847d660dffdb32 1 0SeX300iaPEXtAmSp5rUFEoOKkA (comment: CHECK inhibited_by CHEBI:17191) ------- COMMENT: 89991a632fbaa28c 87 iAe7l1rvE0yND0L+PVO+PaSfu74 (comment: results in retaining specifically modified histone H3 at the genes in question) ------- COMMENT: 89f6dc6bc010963b 1 6uSprN6ZjjDdgcPsaFM4nK91caQ (comment: CHECK double mutant telomere structure phenotype qualitatively different from either single mutant) ------- COMMENT: 89f6dc6bc010963b 2 6uSprN6ZjjDdgcPsaFM4nK91caQ (comment: CHECK double mutant telomere structure phenotype qualitatively different from either single mutant) ------- COMMENT: 89f6dc6bc010963b 29 YRD3sMf+n9l/IVHHTJnlWGX4xT0 (comment: CHECK region between NsiI sites deleted) ------- COMMENT: 89f6dc6bc010963b 30 FGlMKF0gSu/WKuPd5YruYCVrpDA (comment: CHECK truncated at PacI site) ------- COMMENT: 89f6dc6bc010963b 31 eRH2XpZmzPtiEvUHosX1ETeVysw (comment: CHECK region between NdeI and XhoI sites deleted) ------- COMMENT: 8a0da786fa9e1aed 1 KMkKZYd8PbS5ODPaPw9zjVM4qvs (comment: activated by ATP) ------- COMMENT: 8a3c8a8742417b66 1 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 8a3c8a8742417b66 2 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 8a3c8a8742417b66 3 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 8a3c8a8742417b66 4 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 8a3c8a8742417b66 5 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 8a3c8a8742417b66 6 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 8a3c8a8742417b66 7 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 8a3c8a8742417b66 8 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 8a3c8a8742417b66 9 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 8a3c8a8742417b66 10 PtWz3QLtFNOk+hQdKIzxMwXnm5Q Fig. 3A, B ------- COMMENT: 8a3c8a8742417b66 11 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 8a3c8a8742417b66 12 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 8a3c8a8742417b66 13 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 8a3c8a8742417b66 14 q1Wbilz7XLNvKihwplRXI8cTHkw our results indicate that Pob3 function at the centromere does not appear to affect the production or accumulation of both unprocessed transcripts and siRNAs. ------- COMMENT: 8a3c8a8742417b66 15 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 8a3c8a8742417b66 16 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 8a3c8a8742417b66 17 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 8a3c8a8742417b66 18 s0LMevRjNsm+dxiJbpnwPvFignE Fig. S6A ------- COMMENT: 8a3c8a8742417b66 19 ZSoIdAAkba/kthzeLNn7KxqZ/Bw Fig. S6B ------- COMMENT: 8a3c8a8742417b66 20 AoxCF6165PlYpVxgfR9odj3O31Y Fig. S6C ------- COMMENT: 8a3c8a8742417b66 21 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: 8a3c8a8742417b66 22 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: 8a3c8a8742417b66 23 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: 8a3c8a8742417b66 26 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 27 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 28 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 29 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 30 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 31 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 32 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 33 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 34 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 35 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 36 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 37 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 38 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 39 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 40 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 41 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 42 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 43 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 44 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 45 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 46 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 47 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 48 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 49 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 50 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 51 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 52 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 53 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 54 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 55 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 56 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 57 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 58 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 59 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 60 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 61 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 62 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 63 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 64 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 65 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 66 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 67 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 68 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 69 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 70 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 71 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 72 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 73 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 74 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 75 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 76 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 77 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a3c8a8742417b66 78 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 8a6340471870ad39 5 o0qbPtDN0pWqoRZmd/y02O9ZcJU Fig. 2c ------- COMMENT: 8a6340471870ad39 13 c37HjMWoW5QnpfnuO9PILwRxlUk they don't actually use pombe isu1 because they couldn't purify it, but they try both C. thermophilum and S. cerevisiae Isu1 and get similar results & sequence conservation is good to pombe. ------- COMMENT: 8a6340471870ad39 14 c37HjMWoW5QnpfnuO9PILwRxlUk they don't actually use pombe isu1 because they couldn't purify it, but they try both C. thermophilum and S. cerevisiae Isu1 and get similar results & sequence conservation is good to pombe. ------- COMMENT: 8a653c867356cc80 1 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 8a653c867356cc80 2 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 8a653c867356cc80 3 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 8a653c867356cc80 4 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 8a653c867356cc80 5 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 8a653c867356cc80 6 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 8a653c867356cc80 7 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 8a653c867356cc80 8 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 8a653c867356cc80 9 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 8a653c867356cc80 10 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 8a653c867356cc80 11 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 8a653c867356cc80 12 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 8a653c867356cc80 13 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 8a653c867356cc80 14 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 8a653c867356cc80 15 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 8a653c867356cc80 16 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 8a653c867356cc80 17 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 8a653c867356cc80 18 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 8a653c867356cc80 19 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 8a653c867356cc80 20 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 8a653c867356cc80 21 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 8a653c867356cc80 22 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 8a653c867356cc80 23 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 8a66efe1aac64683 1 V8aY9cMSuflWPDnZXK1Ej8D/EEk fig 1b ------- COMMENT: 8a66efe1aac64683 2 iVadS/SnP2wfVOb9aCKtc/aRBXg Fig 1B vw: corrected back to dis2 not cdk9! ------- COMMENT: 8a66efe1aac64683 3 V8aY9cMSuflWPDnZXK1Ej8D/EEk fig 1b ------- COMMENT: 8a66efe1aac64683 4 M9hf5+zj5g8ZyOpygTNTyptK4kc Fig 3e ------- COMMENT: 8a66efe1aac64683 5 aO9T3xnJGkRYlz6mM+BBFGBLfGc (comment: vw: I changed the allele to the multi gene genotype to reflect the comment.) Extended Data Fig 2c Fcp1 inactivation stabilizes Rpb1 Ser2 phosphorylation after Lsk1 inhibition. Fission-yeast strains, lsk1as or lsk1as fcp1-452, were grown at 30 °C and shifted to 37 °C (or not shifted), treated for the indicated time with 20 μM 3-MB-PP1, and analysed by immunoblotting for Pol II Ser2 phosphorylation ------- COMMENT: 8a66efe1aac64683 6 EJDCTdkRx77h2WcfZMUB/nZ3DHA Extended Data Fig 2f .(comment: CHECK (cdk9as, cdk9as ssu72C13S, ssu72C13S)) ------- COMMENT: 8a66efe1aac64683 7 8IjrniGe3UJyAa5E289BRp9RsNk Extended Data Fig 5d . ------- COMMENT: 8a66efe1aac64683 11 Jw4n0vyfD8LN9CSgroS/8BNrs34 Extended Data Fig 6a,b ChIP–qPCR analysis at the rps17a+ gene. Comparison of pSpt5:Spt5 ratio in the indicated strains upstream and downstream of the CPS at 30 °C (left) and comparison of the ratio between dis2+ and dis2-11 cells at 18 °C (right). Extended Data Fig 6a,b ------- COMMENT: 8a66efe1aac64683 12 2qUeH3RNQLEb0nFsW5C2X/z3LVU Extended Data Fig 6e Cdk9 does not restrict chromatin recruitment of Sds21. ------- COMMENT: 8a66efe1aac64683 13 SQ+EBOE9aalQsoLDATg4c9xVmEc Extended Data Fig 4a Cdk9 inhibition increased chromatin recruitment of Dis2 ------- COMMENT: 8a66efe1aac64683 15 Oj3DWdu4BkhwcQ/IVt1bFPQ5ZVU Extended Data Fig 4d (comment: Added Dis2 extension) ------- COMMENT: 8a66efe1aac64683 16 0BRYiaBYgOqhQ/f9yOObgWrBAAc Extended Data Fig 4d (comment: (vw: fixed allele)) ------- COMMENT: 8a66efe1aac64683 17 5YPkZ9xI98cR4EsqbXEnmKSp+qA Extended Data Fig 5a (comment: vw: fixed allele and target) ------- COMMENT: 8a66efe1aac64683 18 5YPkZ9xI98cR4EsqbXEnmKSp+qA Extended Data Fig 5a (comment: vw: fixed allele and target) ------- COMMENT: 8a66efe1aac64683 19 zKaSr7UHaBDVAN0K0H1DI3cqymk Extended Data Fig 5a (comment: vw: based on EXP and comment changed allele from psf2 to cdk9 (P.P. Core CPF recruitment to chromatin is unaffected by Cdk9 inhibition). pfs2 pla1 cft1) ------- COMMENT: 8a66efe1aac64683 20 M9hf5+zj5g8ZyOpygTNTyptK4kc Fig 3e ------- COMMENT: 8a66efe1aac64683 22 ZT/NAYvakEmN06B3uNsDomnH+4o Fig 1b ------- COMMENT: 8a66efe1aac64683 23 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: 8a66efe1aac64683 24 Z+gvfzV4nnjpJNqBl/5+8V5d40k Fig 1c, Extended Data Fig 2a ------- COMMENT: 8a66efe1aac64683 25 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: 8a66efe1aac64683 26 06b6B1WqXBfXU/8ae8sO2XMgE/U Fig. 1d, Extended Data Fig. 1d) ------- COMMENT: 8a66efe1aac64683 27 06b6B1WqXBfXU/8ae8sO2XMgE/U Fig. 1d, Extended Data Fig. 1d) ------- COMMENT: 8a66efe1aac64683 28 jNxywcdh/pf5NvrXNFgqVsfo/zg (comment: check this addition) Fig. 2a, Extended Data Fig. 2a ------- COMMENT: 8a66efe1aac64683 29 TsROi2mvrE44Lmm8+szMl1+DEEw Fig S2, Extended Data Fig. 2c ------- COMMENT: 8a66efe1aac64683 30 0e3dRoT0NqnWXbhEqXwoDrC9+Cs Fig S2, Extended Data Fig. 2d ------- COMMENT: 8a66efe1aac64683 32 HmGP9VrBbip8IDaQBN5QRSa5dXc Fig. 2d, ------- COMMENT: 8a66efe1aac64683 33 R6tsw27EJe3jlq/pY3I5wLhWXlI Extended Data Fig 2f ------- COMMENT: 8a66efe1aac64683 34 R6tsw27EJe3jlq/pY3I5wLhWXlI Extended Data Fig 2f ------- COMMENT: 8a66efe1aac64683 36 9NsfEqlA1QR5eL72H7vfMeTkVf4 Extended Data Fig 3a, Fig. 2c, Extended Data Fig. 3b–d) ------- COMMENT: 8a66efe1aac64683 38 ZU8sUkSYtpv1jUEC6l7/s7dsc9Y Fig. 2e, Extended Data Fig. 4b ------- COMMENT: 8a66efe1aac64683 40 vDf8vOWyMk2RH3OC+l8YB2whniU Fig. 2e, Extended Data Fig. 4c ------- COMMENT: 8a66efe1aac64683 45 ca8/PyubDo3nSmOz2tati9skIq0 fig 6a, b ------- COMMENT: 8a66efe1aac64683 46 ca8/PyubDo3nSmOz2tati9skIq0 fig 6a, b ------- COMMENT: 8a66efe1aac64683 48 TczzOGdG4sRNTlyR8kqIoHZCZWg FIgure 3d. Extended fig 8d (comment: (vw: some suppression?)) ------- COMMENT: 8a66efe1aac64683 49 fz4LFS3CPGl2iE41JYkB4S0Tm14 extended data figure 9 decreased RNA pol2 localization to chromatin (occurs at termination sites) ------- COMMENT: 8a66efe1aac64683 50 H2ZUsZNzolnfw1wxPfUGwc8CoKw Extended data figure 9 ------- COMMENT: 8a66efe1aac64683 51 9NsfEqlA1QR5eL72H7vfMeTkVf4 Extended Data Fig 3a, Fig. 2c, Extended Data Fig. 3b–d) ------- COMMENT: 8a66efe1aac64683 53 Y3l7JuGKQKiGovG7/DBxO+/YP1o figure 3e, Also increased termination index Fig. 4e ------- COMMENT: 8a66efe1aac64683 54 Wg5WazyvHPgJ6g8MTpHt7ixeXNw figure 3e ------- COMMENT: 8a66efe1aac64683 55 3IIZKd5m6W0pGR2ur3U7rMT0A9A fig 1d ------- COMMENT: 8a66efe1aac64683 56 3IIZKd5m6W0pGR2ur3U7rMT0A9A fig 1d ------- COMMENT: 8a66efe1aac64683 57 3IIZKd5m6W0pGR2ur3U7rMT0A9A fig 1d ------- COMMENT: 8a66efe1aac64683 58 cVAzkK3zDElVbx6tZv6XPRHtlnM Fig 5c. ------- COMMENT: 8a66efe1aac64683 59 2qajYXZruUR+x5PO0T5FLIqHxzA Extended Data Fig 10 ------- COMMENT: 8a66efe1aac64683 60 2qajYXZruUR+x5PO0T5FLIqHxzA Extended Data Fig 10 ------- COMMENT: 8a66efe1aac64683 61 2qajYXZruUR+x5PO0T5FLIqHxzA Extended Data Fig 10 ------- COMMENT: 8a93d8122afe38cd 1 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 8a93d8122afe38cd 2 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 8a93d8122afe38cd 3 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 8a93d8122afe38cd 4 YFzBr39cewfGsPKoh3UJd1VZqIo (Fig. 4A, Fig. EV2 and EV3) ------- COMMENT: 8a93d8122afe38cd 5 9Oq73bIhZMSkApdqEIPcLSS6SDY (Fig. 3A, Fig. EV1A) ------- COMMENT: 8a93d8122afe38cd 6 qG6B4/iygbuzbDTNpYYepr+6QcM (Fig. 4A, Fig. EV2) ------- COMMENT: 8a93d8122afe38cd 7 XuvUOKbZQdA8jGDmVVmz2iuY+UI (Fig. EV4) ------- COMMENT: 8a93d8122afe38cd 8 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: 8a93d8122afe38cd 9 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: 8a93d8122afe38cd 10 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 8a93d8122afe38cd 11 YFzBr39cewfGsPKoh3UJd1VZqIo (Fig. 4A, Fig. EV2 and EV3) ------- COMMENT: 8a93d8122afe38cd 12 9Oq73bIhZMSkApdqEIPcLSS6SDY (Fig. 3A, Fig. EV1A) ------- COMMENT: 8a93d8122afe38cd 13 qG6B4/iygbuzbDTNpYYepr+6QcM (Fig. 4A, Fig. EV2) ------- COMMENT: 8a93d8122afe38cd 14 XuvUOKbZQdA8jGDmVVmz2iuY+UI (Fig. EV4) ------- COMMENT: 8a93d8122afe38cd 15 kb7NOBRiqlmzY3vQrPFeR5NkglI (Fig. 7C) ------- COMMENT: 8a93d8122afe38cd 16 v6UHEdyRVNwZ2qyI+J9KpwaNVKQ The mobility shift observed in Phos-tag gels was almost completely abolished in this EGFP-Sfr1-7A mutant protein, indicating that it was related to modifications in these residues of the protein (Fig. 1D). ------- COMMENT: 8a93d8122afe38cd 17 AZkNyt6hRyyDl+KCz1eh2ak1x6g (Fig. S4A) ------- COMMENT: 8a93d8122afe38cd 18 NuNHLmtoq4GzF6sDw6D9LwteRCo (Fig. S4B) ------- COMMENT: 8a93d8122afe38cd 19 38JLmwB14uYdFmDsenSDv0PccWU (Fig. 4, Fig. EV2) ------- COMMENT: 8a93d8122afe38cd 20 j30KwK8QbZkLhoky9eRwFbLzgAY (Fig. S4C) ------- COMMENT: 8a93d8122afe38cd 21 D8FaEDlsZI9q7iZa1n3/xZenzgg (Fig. S3A and B) ------- COMMENT: 8a93d8122afe38cd 22 D8FaEDlsZI9q7iZa1n3/xZenzgg (Fig. S3A and B) ------- COMMENT: 8a93d8122afe38cd 23 D8FaEDlsZI9q7iZa1n3/xZenzgg (Fig. S3A and B) ------- COMMENT: 8a93d8122afe38cd 24 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: 8a93d8122afe38cd 28 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: 8a93d8122afe38cd 29 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: 8a93d8122afe38cd 30 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 8a93d8122afe38cd 31 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 8a93d8122afe38cd 32 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: 8a93d8122afe38cd 34 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 8a93d8122afe38cd 35 wVOVtTym3UG/na5CQkcvuOXkP7Q (Fig. 4A, Fig. S2) ------- COMMENT: 8a93d8122afe38cd 37 khsisV/+a3EzkwQ6iLhnNm1HrpM (Fig. 6) ------- COMMENT: 8a93d8122afe38cd 40 tydJs/e79FU6St5o7YEqIS3rSks (Fig. 1 and 5) ------- COMMENT: 8a93d8122afe38cd 41 oii3W+9T6tN00ECmfiW4sRniC6w EGFP-Sfr1 protein was clearly detected from the end of S-phase (2.5 h after meiotic induction) to the entry into the first chromosome segregation (meiosis I, 4.5 h), with the highest levels during meiotic prophase (Fig. 1A). ------- COMMENT: 8a93d8122afe38cd 42 kb7NOBRiqlmzY3vQrPFeR5NkglI (Fig. 7C) ------- COMMENT: 8a93d8122afe38cd 43 kb7NOBRiqlmzY3vQrPFeR5NkglI (Fig. 7C) ------- COMMENT: 8a93d8122afe38cd 45 zOWVHh2W040Fd2ZkPEcqVaa2r70 (Fig. 1B and 2C) ------- COMMENT: 8a93d8122afe38cd 46 3T8i+bYtS2y41hvQE6M1DUDurGw This mobility shift was diminished in cells of the same culture treated in parallel with 1-NM- PP1, showing a 50% reduction in the slow-migrating band compared to DMSO-treated cells at 4 h after meiotic induction (n = 4, P value = 0.0337). This result suggests that Cdc2 is responsible for the phosphorylation of Sfr1 during meiotic prophase. (Fig. 2A) ------- COMMENT: 8a93d8122afe38cd 47 wVOVtTym3UG/na5CQkcvuOXkP7Q (Fig. 4A, Fig. S2) ------- COMMENT: 8a93d8122afe38cd 48 wVOVtTym3UG/na5CQkcvuOXkP7Q (Fig. 4A, Fig. S2) ------- COMMENT: 8a93d8122afe38cd 49 kuRdbpnFrU9sjksgW9GQwEQ1424 (Fig. 5) ------- COMMENT: 8a93d8122afe38cd 50 khsisV/+a3EzkwQ6iLhnNm1HrpM (Fig. 6) ------- COMMENT: 8a93d8122afe38cd 52 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 8a93d8122afe38cd 53 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: 8a93d8122afe38cd 54 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: 8a93d8122afe38cd 55 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: 8a93d8122afe38cd 56 kb7NOBRiqlmzY3vQrPFeR5NkglI (Fig. 7C) ------- COMMENT: 8a93d8122afe38cd 57 kb7NOBRiqlmzY3vQrPFeR5NkglI (Fig. 7C) ------- COMMENT: 8a93d8122afe38cd 58 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 8a93d8122afe38cd 60 gRK0bumYnCVPmKXmgeHPXg/LLNc (Fig. S3C) ------- COMMENT: 8a93d8122afe38cd 61 kuRdbpnFrU9sjksgW9GQwEQ1424 (Fig. 5) ------- COMMENT: 8a93d8122afe38cd 62 gRK0bumYnCVPmKXmgeHPXg/LLNc (Fig. S3C) ------- COMMENT: 8a93d8122afe38cd 63 kuRdbpnFrU9sjksgW9GQwEQ1424 (Fig. 5) ------- COMMENT: 8a93d8122afe38cd 64 gRK0bumYnCVPmKXmgeHPXg/LLNc (Fig. S3C) ------- COMMENT: 8a93d8122afe38cd 65 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: 8ac46a804d130afe 1 ggdpDvEiBIIHCTQA6vbnSfyoa90 ) Rad21 locates to centromere in dfp1-3A mutants. ------- COMMENT: 8ac46a804d130afe 2 dSPX6y6WOfJsnFOv644TSmy1+6M (comment: (vw: changed genotype to add swi6 delt)) dfp1-CFP-2CD restores lagging chromosomes in the absence of Swi6. Rad21 locates to centromere in dfp1-CFP-2CD mutants. ------- COMMENT: 8ac46a804d130afe 3 f3pnhgDBAhf+jFjX+vcDV1wCdkc (Figure 3B) Chp1 fails to accumulate at noncentromeric location in the absence of Chp2 and Swi6. ------- COMMENT: 8ac46a804d130afe 4 tB9W29P1VkeX76XvactEM9UkWUM (Figure 3B) (comment: vw changed more specific to lagging chromosmes) Increase the frequency of mitotic cells showing lagging chromosomes. Rad21 fails to accumulate at centromere in the absence of Swi6. ------- COMMENT: 8ac46a804d130afe 5 sLl2zcxTxxGAtDkaYaTcbSD7nS8 Fig 7 (comment: using minichromosome) ------- COMMENT: 8ac46a804d130afe 6 sLl2zcxTxxGAtDkaYaTcbSD7nS8 Fig 7 (comment: using minichromosome) ------- COMMENT: 8ac46a804d130afe 7 sLl2zcxTxxGAtDkaYaTcbSD7nS8 Fig 7 (comment: using minichromosome) ------- COMMENT: 8ac46a804d130afe 8 UnQw94nU5T+QzGDesDT6Zls7XHA fig 7B ------- COMMENT: 8ac46a804d130afe 9 sJQr2wKA4JZdSpWnZ3qr4VqJ9Hw fig 7B ------- COMMENT: 8ac46a804d130afe 10 kTvB5rbZANgdq/lfui571ZaBluI Fig 7B ------- COMMENT: 8ac46a804d130afe 11 K8J3Ts6CLLIzv5gCTYxHWk2S0u8 Fig. 5B (comment: CHECK abolish Swi6 protein localization to centromere during vegetative growth) ------- COMMENT: 8ac46a804d130afe 13 xX6YaC9Q9r2nodU7YzzTAj/ihYs (comment: (VW changed to multi allele)) The delay of m-to-G1/S phase transition in swi6∆ and dfp1-3A was abolished after deleting mad2. ------- COMMENT: 8ac46a804d130afe 16 akTkUEBbgacJ646b3AQ12QF2z/M Fig. 2A (comment: Spindle pole-to-pole distance was measured based on the distance of duplicated SPBs revealed by Sad1-DsRed.) ------- COMMENT: 8ac46a804d130afe 18 V1nnM7f8PmcbzyWgPa2QIIN3gNE (comment: (vw 3B? changed from normal to lagging, added penetrance)) dfp1-CFP-2CD rescues minichromosome loss in the absence of Swi6. ------- COMMENT: 8ac46a804d130afe 19 akTkUEBbgacJ646b3AQ12QF2z/M Fig. 2A (comment: Spindle pole-to-pole distance was measured based on the distance of duplicated SPBs revealed by Sad1-DsRed.) ------- COMMENT: 8ac46a804d130afe 20 0gHc17GFWDwnmi2q5LtiXm9TDl8 Fig. 2C ------- COMMENT: 8ac46a804d130afe 21 0gHc17GFWDwnmi2q5LtiXm9TDl8 Fig. 2C ------- COMMENT: 8ac46a804d130afe 22 0gHc17GFWDwnmi2q5LtiXm9TDl8 Fig. 2C ------- COMMENT: 8ac46a804d130afe 25 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 8ac46a804d130afe 26 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: 8ac46a804d130afe 27 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 8ac46a804d130afe 28 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 8ac46a804d130afe 29 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: 8ac46a804d130afe 30 48scMHgSmB2K43R1mHE+8bvHNmo Fig. S1 ------- COMMENT: 8ac46a804d130afe 32 2cOqTmW3huCWJhEAtk2iWb2EJjU Fig. 5B ------- COMMENT: 8ac46a804d130afe 33 fOtoaXyhmUGmVAhfe2+kB2F1fq4 Fig. 5E ------- COMMENT: 8ac46a804d130afe 35 pqPnkrRQwjEW6xsms+hgbdsXzFw Fig. 5C ------- COMMENT: 8ac46a804d130afe 38 sLl2zcxTxxGAtDkaYaTcbSD7nS8 Fig 7 (comment: using minichromosome) ------- COMMENT: 8ac46a804d130afe 39 puKgaCb894GSgUExJ3gx3kz0l28 The swi6-sm1 allele disrupts silencing without lagging chro- mosomes (Yamagishi et al. 2008) (Figure S4, A and B). We observed a similar frequency of lagging chromosomes in wild-type (1%) and swi6-sm1 mutants (1.03%). ------- COMMENT: 8ac46a804d130afe 40 OH/67G1ErCbqtMzoyVoplxvMipY Rad21-GFP enrichment at the centromere is unaffected in swi6-sm1 (Figure S4C) ------- COMMENT: 8ac46a804d130afe 41 UnQw94nU5T+QzGDesDT6Zls7XHA fig 7B ------- COMMENT: 8ac46a804d130afe 42 UnQw94nU5T+QzGDesDT6Zls7XHA fig 7B ------- COMMENT: 8acb880886aa8d18 19 nk5qMlXMVcZYOTkbvSLuumt1tAM kinetochore localization requires MIND complex ------- COMMENT: 8acb880886aa8d18 33 CL0ezVECo5Hw8AMAcufjlSQcyNs implies that MIND complex is required for Sos7 to localize to the kinetochore ------- COMMENT: 8ad14ac446a38d4f 8 WOZDQPaEiySJdZanXoQOHw3wJ54 Pom1 does not relocalize to cell sides ------- COMMENT: 8ad14ac446a38d4f 9 WOZDQPaEiySJdZanXoQOHw3wJ54 Pom1 does not relocalize to cell sides ------- COMMENT: 8ad14ac446a38d4f 10 WOZDQPaEiySJdZanXoQOHw3wJ54 Pom1 does not relocalize to cell sides ------- COMMENT: 8ad14ac446a38d4f 11 WOZDQPaEiySJdZanXoQOHw3wJ54 Pom1 does not relocalize to cell sides ------- COMMENT: 8ad14ac446a38d4f 12 F25bX6PEg+7qnPdVjdZAU5q2aoo absent when glucose limited ------- COMMENT: 8ad14ac446a38d4f 14 WOZDQPaEiySJdZanXoQOHw3wJ54 Pom1 does not relocalize to cell sides ------- COMMENT: 8ad14ac446a38d4f 15 QyeRiMRvTz1LzknwXAnZ+rgdefo Tea4 does not relocalize to cell sides ------- COMMENT: 8ad14ac446a38d4f 16 1NT3P5/O/mo8rcp/mtatdKM1rCw (comment: CHECK microtubule dynamics rescued) ------- COMMENT: 8ad14ac446a38d4f 18 6GS38MCQimKqfeEvqsVAiVJC/K4 (comment: CHECK through negative regulation of Cls1) ------- COMMENT: 8ad14ac446a38d4f 20 9tJP+1C8LAULT0Pzd+iBin83stE Pom1 relocalizes to cell sides ------- COMMENT: 8ad14ac446a38d4f 21 9tJP+1C8LAULT0Pzd+iBin83stE Pom1 relocalizes to cell sides ------- COMMENT: 8ad14ac446a38d4f 22 9tJP+1C8LAULT0Pzd+iBin83stE Pom1 relocalizes to cell sides ------- COMMENT: 8ad14ac446a38d4f 23 9tJP+1C8LAULT0Pzd+iBin83stE Pom1 relocalizes to cell sides ------- COMMENT: 8ad14ac446a38d4f 24 9tJP+1C8LAULT0Pzd+iBin83stE Pom1 relocalizes to cell sides ------- COMMENT: 8ad14ac446a38d4f 26 V92BlEYOaGIe20XKKh7h04zQnDQ Pom1 relocalization to cell sides ------- COMMENT: 8ad14ac446a38d4f 28 WOZDQPaEiySJdZanXoQOHw3wJ54 Pom1 does not relocalize to cell sides ------- COMMENT: 8ad14ac446a38d4f 29 WOZDQPaEiySJdZanXoQOHw3wJ54 Pom1 does not relocalize to cell sides ------- COMMENT: 8ad9bcb87e5ff032 3 mbheR9uFmdp1p8IDbA/OIPg+sJI (comment: CHECK convert to double mutant (cnp1 overexpression)) ------- COMMENT: 8ad9bcb87e5ff032 5 xQsfALYtJQDXGoVGPqi/m3i/WYU (comment: central core) ------- COMMENT: 8ad9bcb87e5ff032 33 xQsfALYtJQDXGoVGPqi/m3i/WYU (comment: central core) ------- COMMENT: 8b51c7a94991f857 4 sp+YKda8NeVhCAzVsZAaLWCkNSE indicated by high level of H1 kinase activity ------- COMMENT: 8b51c7a94991f857 5 WeK7yxLE1WYcbMMFfcCh4W0c0Ns Fig. 2D ------- COMMENT: 8b51c7a94991f857 6 aDDkf0LunWdqVxgyZq/tqXiu8zY Fig. 2E ------- COMMENT: 8b51c7a94991f857 8 aDDkf0LunWdqVxgyZq/tqXiu8zY Fig. 2E ------- COMMENT: 8b5476ba0daf2d2d 1 OMNA5QZVl21HnwDcnIKd2awbvIc fig 1F ------- COMMENT: 8b5476ba0daf2d2d 2 FE6zuZbGiSBq/0/SH7GfHwpnv+k cells lacking Sty1 grew in these low LatA concentrations (Figure 1B). ------- COMMENT: 8b5476ba0daf2d2d 9 txMGZUn2chO1FIUwaxq3xMdFaKk fig. 1 Supp5 ------- COMMENT: 8b5476ba0daf2d2d 10 gp17BSgEpOEuAa745WFepeWrabI fig 1 SuppF ------- COMMENT: 8b5476ba0daf2d2d 11 2JgqEhtO9CI9QCJdBcMALdKyr2c upstream elements of this signaling cascade shared this phenotype... Mcs4, the redundant MAPKKK´s Wak1 and Win1, and MAPKK Wis1 (Figure 1A, Figure 1—figure supplement 1 ------- COMMENT: 8b5476ba0daf2d2d 20 OYNkWkP5WWhBf1rO2p7VPJT5mrM fig2 supp1 ------- COMMENT: 8b5476ba0daf2d2d 21 4yCVBSkl6bg2aTrDdCZ1ZS1USlw fig 5b ------- COMMENT: 8b5476ba0daf2d2d 24 4yCVBSkl6bg2aTrDdCZ1ZS1USlw fig 5b ------- COMMENT: 8b5476ba0daf2d2d 25 XhFk42+F7J5SyY4h37VUbpqsXKs (comment: CHECK 25%) ------- COMMENT: 8b5476ba0daf2d2d 28 m2lDTj7/5DSh4hMqcYuT5wB/oiA explicit delay in ring constriction and disassembly (21 ± 0.6 min in wild-type cells vs 36 ± 1.6 min in for3D cells; Figure 2—figure supplement 5 ------- COMMENT: 8b5476ba0daf2d2d 29 nnuWhrFU7jaQ2CsXT4A21I2oLbM fig 3a ------- COMMENT: 8b5476ba0daf2d2d 30 ltrQkoUJm6Wn2k5av1wL1F55HgU fig 3b ------- COMMENT: 8b5476ba0daf2d2d 36 vZoGti4XgtXZm493hllUtUYMSRg (comment: CHECK Overexpression under the control of B-estradiol promoter (vw: I added an allele synonym, later these will be searchable and visible)) ------- COMMENT: 8b5476ba0daf2d2d 37 ZQbBWnqnB6OP4e6B4j3k7jJOdLY (Figure 2—figure supplement 5). ngs formed and constricted correctly in >85% of sty1D cells (Figure 2D). ------- COMMENT: 8b5476ba0daf2d2d 41 y/tijFQF5K4U3oKbL/BwGFa57sQ Figure 2A and C) (comment: CHECK check, has synonym increased stability (better than increased length?)) ------- COMMENT: 8b5476ba0daf2d2d 42 KntnJkiXmLt+bu4ukaXFz0c82Wk figure 5B ------- COMMENT: 8b5476ba0daf2d2d 43 tG2m7DcDCbkRgV/mQIIH/2UD5rY (comment: CHECK 50%) ------- COMMENT: 8b5476ba0daf2d2d 45 qW0fooJwQWtfFzpmyf80rEliP/A fig 5c ------- COMMENT: 8b5476ba0daf2d2d 48 Zgwtj0cv+dVSsubDF2lbtPEuWxw Cells expressing a mutant allele Mcs4(D512N) that does not activate the SAPK pathway upon stimulation with hydro- gen peroxide (Shieh et al., 1997), displayed Sty1 activation during LatA treatment, ------- COMMENT: 8b5476ba0daf2d2d 49 2JgqEhtO9CI9QCJdBcMALdKyr2c upstream elements of this signaling cascade shared this phenotype... Mcs4, the redundant MAPKKK´s Wak1 and Win1, and MAPKK Wis1 (Figure 1A, Figure 1—figure supplement 1 ------- COMMENT: 8b5476ba0daf2d2d 50 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 8b5476ba0daf2d2d 51 gp17BSgEpOEuAa745WFepeWrabI fig1 SuppF ------- COMMENT: 8b5476ba0daf2d2d 52 gp17BSgEpOEuAa745WFepeWrabI fig1 SuppF ------- COMMENT: 8b5476ba0daf2d2d 53 nnuWhrFU7jaQ2CsXT4A21I2oLbM fig 3a ------- COMMENT: 8b5476ba0daf2d2d 54 7EN/W7J56AkhkjSK6NvdsePEkSk fig 3c ------- COMMENT: 8b5476ba0daf2d2d 55 c0uMiNqcE3BgpszPax9cHtCbVbg fig 4d ------- COMMENT: 8b5476ba0daf2d2d 56 ukb1VO4g9bYLoqcV9BLOixAHEbk We found that total For3 levels also decline in S. pombe wild-type cells in response to stimuli that activate Sty1, like heat shock (40 ̊C), osmotic saline (0.6 M KCl), and oxidative stress (1 mM H2O2) (Pe ́rez and Cansado, 2010) in a MAPK-dependent manner (Figure 5—figure supplement 3). ------- COMMENT: 8b5476ba0daf2d2d 57 ukb1VO4g9bYLoqcV9BLOixAHEbk We found that total For3 levels also decline in S. pombe wild-type cells in response to stimuli that activate Sty1, like heat shock (40 ̊C), osmotic saline (0.6 M KCl), and oxidative stress (1 mM H2O2) (Pe ́rez and Cansado, 2010) in a MAPK-dependent manner (Figure 5—figure supplement 3). ------- COMMENT: 8b5476ba0daf2d2d 58 ukb1VO4g9bYLoqcV9BLOixAHEbk We found that total For3 levels also decline in S. pombe wild-type cells in response to stimuli that activate Sty1, like heat shock (40 ̊C), osmotic saline (0.6 M KCl), and oxidative stress (1 mM H2O2) (Pe ́rez and Cansado, 2010) in a MAPK-dependent manner (Figure 5—figure supplement 3). ------- COMMENT: 8b5476ba0daf2d2d 59 j5plG9Kwrx8k+uBIUwapPtkcjiA Figure 5—figure supplement 4 ------- COMMENT: 8b5476ba0daf2d2d 60 j5plG9Kwrx8k+uBIUwapPtkcjiA Figure 5—figure supplement 4 ------- COMMENT: 8b5476ba0daf2d2d 62 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 8b5476ba0daf2d2d 63 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 8b5476ba0daf2d2d 64 P3W+2oA5vdGOnCudaiQuI0u4xN8 (comment: CHECK replaces wt annotation) ------- COMMENT: 8b5476ba0daf2d2d 66 P3W+2oA5vdGOnCudaiQuI0u4xN8 (comment: CHECK replaces wt annotation) ------- COMMENT: 8b6f74049ec3405d 1 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: 8b6f74049ec3405d 2 PmWoqbQNc+GnOIqQDTOz63HDm2s (APC) activation occurred and chromosome cohesion was lost (Figure 1A and 1B). ------- COMMENT: 8b6f74049ec3405d 3 PmWoqbQNc+GnOIqQDTOz63HDm2s (APC) activation occurred and chromosome cohesion was lost (Figure 1A and 1B). ------- COMMENT: 8b6f74049ec3405d 7 +Q7IhIu/Mr5HR7hKvPZpl1DtdAY Figure S4 /Figure 3A and 3B, and Video S3) ------- COMMENT: 8b6f74049ec3405d 8 YXWsv4jcxCr03CfCX+cTX5yvL+E Figure S4) ------- COMMENT: 8b6f74049ec3405d 9 mq0Uhx73hldQjZkG8hvtWQseK+U Figure 2C (comment: check (also nuclear envelope protrusion?) ------- COMMENT: 8b6f74049ec3405d 10 EIGjLZm263nLLLa0aiwUyQlHvus Figure 3A and 3B, and Video S3) ------- COMMENT: 8b6f74049ec3405d 11 cDXQ4ZZ9yIXm/X8AviPkhr+6oY8 (Figure 1). ------- COMMENT: 8b6f74049ec3405d 12 bJKDrWNX7AHJFQi9ezE4Uxdy0L8 Fig. 4G ------- COMMENT: 8b6f74049ec3405d 13 vmEJiaZHWs8NOGPIR/b25oDdqHU Figure 6C ------- COMMENT: 8b6f74049ec3405d 14 Iqhbd5nYEECxkfBJKKBiAING3Ws Figure 6C (ablated Nuclear envelope) ------- COMMENT: 8b7a4bf27652b9d5 12 HgJodLmIfbQiEnUZ/47NUXPlgGk Consistently, mutations in the php3􏰀 - and php5􏰀 -encoded CCAAT-binding proteins were phenocopies of php2􏰂 ------- COMMENT: 8b7a4bf27652b9d5 13 HgJodLmIfbQiEnUZ/47NUXPlgGk Consistently, mutations in the php3􏰀 - and php5􏰀 -encoded CCAAT-binding proteins were phenocopies of php2􏰂 ------- COMMENT: 8b7a4bf27652b9d5 14 F62KvGgnp7fbYopogjhGB4ixHow Fig.n 6b ------- COMMENT: 8b7a4bf27652b9d5 16 GHTCOTEOOuZS5ufdGBIeaYUW7zI using the cross-linking agent EGS, we found that the Php4 protein associates with the Php2/Php3/Php5 complex ------- COMMENT: 8b8d3cc2e5f5c371 2 l165mgamoT9Y90wthcjZQM/bn5E table 1 ------- COMMENT: 8b8d3cc2e5f5c371 5 Ya1ePZe7coVca74VKVwFN2qy9xc we failed to detect a band corresponding to the full-length Nup189 fused with GFP (Nup98–Nup96–GFP), indicating that autocleavage occurs with no remains of the joint molecule. The same bands corresponding to Nup98 and Nup96-GFP were also detected in the splicing-defective mutant, as expected (Fig. 2B and C, ------- COMMENT: 8b91b02e39a87d36 1 b3uouz/R/Afl3Mf7bQriOepDTUg We used Dcp2 constructs of increasing length (Figure 1D) and found that a Dcp2 region located between residues 255 and 266 is required for the interaction with Edc3 (Figure 1D, lane 3 versus lanes 1 and 2). B ------- COMMENT: 8b91b02e39a87d36 2 iWyNvUys+HS8JYcHiyqPCNogygk In summary, our data show that the Edc3 and Scd6 LSm domains compete for the same Dcp2- binding motifs and that both interactions are mutually exclusive ------- COMMENT: 8b91b02e39a87d36 7 lJWh/oU8xch24KKtvOsrodMwICU (Figure 6A) ------- COMMENT: 8b91b02e39a87d36 13 1QuFLyFqXY6gte0lmwFKPywoFq0 (Supplementary Figure S8C ------- COMMENT: 8bbc6f872ab18094 1 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: 8bbc6f872ab18094 4 PtGDdfoCajveO68urdvZmuK6VhE In prior work the mutant cells appeared to assemble normal contractile rings, but our quantitative measurements revealed that the cdc12-4A mutation reduced by about half both the rate of accumulation and the peak numbers of polymerized actin in the ring (Fig. 2E and Table 1). ------- COMMENT: 8bbc6f872ab18094 5 5UdueyaVjCZnPKA7cai1ZgCkGNo Contractile rings of adf1-M3 mutant cells accumulated actin twice as fast over a similar period of time as wild-type cells (Table 2). ------- COMMENT: 8bbc6f872ab18094 6 Q0fZ8PVegQItHDr7xGt3NSrpAFs Therefore, mature rings of the mutant had on average about 1.9 times as much actin as wild-type cells ------- COMMENT: 8bbc6f872ab18094 7 iKqa5ALcwOCo6RhFWbd7A3qlgYM (Fig. 3C) The number of Cdc12-3GFP molecules in the contractile rings of adf1-M3 mutant cells was on average about twice that of wild-type cells and much more variable ------- COMMENT: 8bbc6f872ab18094 9 jOyo+vUoQVgxkEb7qzz/9UURYaA (Fig. 3A-B and Table 2). Contractile rings of adf1-M3 mutant cells that were able to constrict had twice as many myosin molecules as the wild-type cells, translating to one myosin motor domain for every 70 nm of filament ------- COMMENT: 8bbc6f872ab18094 10 jOyo+vUoQVgxkEb7qzz/9UURYaA (Fig. 3A-B and Table 2). Contractile rings of adf1-M3 mutant cells that were able to constrict had twice as many myosin molecules as the wild-type cells, translating to one myosin motor domain for every 70 nm of filament ------- COMMENT: 8bbc6f872ab18094 11 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: 8bbc6f872ab18094 12 c8iyUeLVtNnttgbDRHyIFz0wyYs Second, the normalized disassembly rate, which took the number of actin molecules in the ring into consideration, was 40% lower in the mutant than wild-type cells. ------- COMMENT: 8bbc6f872ab18094 13 tD99HerYa20DmSFYHW2w9QlCJ+s In contrast, these myosins persisted at nearly their highest levels for an hour and the time course of the process was much more variable in the adf1-M3 mutant cells (Fig. 4C and D). In a few cofilin mutant cells, the myosins dwelled at the cell division site for more than 10 minutes after the completion of the ring constriction (Fig. 5A). ------- COMMENT: 8bbc6f872ab18094 15 9hDTQf31RzAD6UbOcPavKI4PS2o Mutations of either type II myosin gene in the myo2-E1 or myp2Δ strains reduced the numbers of actin molecules in contractile rings by more than half compared with wild-type cells at the end of 10 the maturation period and the onset of constriction (Fig. 6A-B and Table 1). ------- COMMENT: 8bbc6f872ab18094 16 9hDTQf31RzAD6UbOcPavKI4PS2o Mutations of either type II myosin gene in the myo2-E1 or myp2Δ strains reduced the numbers of actin molecules in contractile rings by more than half compared with wild-type cells at the end of 10 the maturation period and the onset of constriction (Fig. 6A-B and Table 1). ------- COMMENT: 8bbc6f872ab18094 17 KsecWmeJA2b5ZiK2nx4a8kzmOBQ (comment: CHECK WHY ISNNT THIS PART. OF ????FYPO:0000230 abnormal actomyosin contractile ring actin filament organization) ------- COMMENT: 8bbc6f872ab18094 18 KsecWmeJA2b5ZiK2nx4a8kzmOBQ (comment: CHECK WHY ISNNT THIS PART. OF ????FYPO:0000230 abnormal actomyosin contractile ring actin filament organization) ------- COMMENT: 8bbc6f872ab18094 19 hUPztZhW8wyvgxgLV96W76CoCaY We conclude that type II myosins contribute to both the assembly and disassembly of actin filaments in contractile rings. ------- COMMENT: 8bbc6f872ab18094 20 hUPztZhW8wyvgxgLV96W76CoCaY We conclude that type II myosins contribute to both the assembly and disassembly of actin filaments in contractile rings. ------- COMMENT: 8bbc6f872ab18094 21 hUPztZhW8wyvgxgLV96W76CoCaY We conclude that type II myosins contribute to both the assembly and disassembly of actin filaments in contractile rings. ------- COMMENT: 8bbc6f872ab18094 22 hUPztZhW8wyvgxgLV96W76CoCaY We conclude that type II myosins contribute to both the assembly and disassembly of actin filaments in contractile rings. ------- COMMENT: 8bbf965b2fdf46b6 1 E9c9PF6Tcpe8eqhY4ak9xFEwdUE (comment: I changed the evidence from IDA to IMP /AL) ------- COMMENT: 8bbf965b2fdf46b6 2 IBz2qm5bhU5o72VoANdo5hDQIg8 Deletion of both homologues fex1 and fex2 make cells highly sensitive to fluoride. Expression of fex1 from a plasmid in fex1Del/fex2Del double deletion mutant rescues fluoride sensitivity. ------- COMMENT: 8bbf965b2fdf46b6 3 E9c9PF6Tcpe8eqhY4ak9xFEwdUE (comment: I changed the evidence from IDA to IMP /AL) ------- COMMENT: 8bbf965b2fdf46b6 4 XeguU2lQXqk/h/rwN3OqKYIjTkc Expression of fex1 from a plasmid in fex1Del/fex2Del double deletion mutant rescues fluoride sensitivity. ------- COMMENT: 8bbf965b2fdf46b6 5 WxuOjivtBrnTH1mpn7JTsZoQ9Ug fig S2, 3 ------- COMMENT: 8bbf965b2fdf46b6 6 WxuOjivtBrnTH1mpn7JTsZoQ9Ug fig S2, 3 ------- COMMENT: 8bbf965b2fdf46b6 7 WxuOjivtBrnTH1mpn7JTsZoQ9Ug fig S2, 3 ------- COMMENT: 8bbf965b2fdf46b6 8 OHWp5mbBnqnCCXYjMEUJhXxCslo Figures 2 and 3A, B ------- COMMENT: 8bbf965b2fdf46b6 10 WxuOjivtBrnTH1mpn7JTsZoQ9Ug fig S2, 3 ------- COMMENT: 8bd8f9428a9ef0ef 1 +scXnx3yc6w/Sw8cgdLzz+BcuQI (comment: Work with Dpb4 for parental histone H3-H4 transfer on the leading strand) ------- COMMENT: 8bd8f9428a9ef0ef 2 asQ9OuAgMOhSc1VeZ4fmpFbvBFs (comment: Work with Dpb3 for parental histone H3-H4 transfer on the leading strand) ------- COMMENT: 8bd8f9428a9ef0ef 3 XMsFCwhI1nyuGtvJAXM5Tw79Z0A (comment: Transfers parental histone H3-H4 on the lagging strand.) ------- COMMENT: 8bd8f9428a9ef0ef 4 KcsyMIzYtO3afzfhOiZZod1/5IA (comment: Deposits parental histone H3-H4 on both daughter strands during DNA replication.) ------- COMMENT: 8bd8f9428a9ef0ef 5 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 8bd8f9428a9ef0ef 6 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 8bd8f9428a9ef0ef 7 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 8bd8f9428a9ef0ef 8 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 8bd8f9428a9ef0ef 9 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 8bd8f9428a9ef0ef 10 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 8bd8f9428a9ef0ef 11 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 8bd8f9428a9ef0ef 12 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 8bd8f9428a9ef0ef 13 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 8bd8f9428a9ef0ef 14 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: 8bd8f9428a9ef0ef 15 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 8bd8f9428a9ef0ef 16 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 8bd8f9428a9ef0ef 17 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 8bd8f9428a9ef0ef 18 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 8bd8f9428a9ef0ef 19 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 8bd8f9428a9ef0ef 20 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: 8bd8f9428a9ef0ef 21 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: 8bd8f9428a9ef0ef 22 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: 8bd8f9428a9ef0ef 23 tOmOPs9JendY5r39VoSXnsV78ik Fig. 3F ------- COMMENT: 8bd8f9428a9ef0ef 24 tOmOPs9JendY5r39VoSXnsV78ik Fig. 3F ------- COMMENT: 8bd8f9428a9ef0ef 25 tOmOPs9JendY5r39VoSXnsV78ik Fig. 3F ------- COMMENT: 8bd8f9428a9ef0ef 26 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: 8bd8f9428a9ef0ef 29 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 8bd8f9428a9ef0ef 30 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 8bd8f9428a9ef0ef 31 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 8bd8f9428a9ef0ef 32 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 8bd8f9428a9ef0ef 33 7jcBxO0h2UrMIaQPBRF4i8t2X9M Fig. 6B, C and D ------- COMMENT: 8bd8f9428a9ef0ef 34 7jcBxO0h2UrMIaQPBRF4i8t2X9M Fig. 6B, C and D ------- COMMENT: 8bd8f9428a9ef0ef 35 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: 8bd8f9428a9ef0ef 36 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 8bd8f9428a9ef0ef 37 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 8bd8f9428a9ef0ef 38 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 8bd8f9428a9ef0ef 39 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 8bd8f9428a9ef0ef 40 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 8bd8f9428a9ef0ef 41 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 8bd8f9428a9ef0ef 42 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 8bd8f9428a9ef0ef 43 2BgYhS2/liLf73JDBHL1ddxHYq8 (comment: Deposits parental histone H3-H4 on both daughter strands during DNA replication) ------- COMMENT: 8bd8f9428a9ef0ef 44 Kt0VnvS5NcgvzzqO3NcL2cvWUBg (comment: Works with Dpb3 for parental histone H3-H4 transfer on the leading strand) ------- COMMENT: 8bd8f9428a9ef0ef 45 XMsFCwhI1nyuGtvJAXM5Tw79Z0A (comment: Transfers parental histone H3-H4 on the lagging strand.) ------- COMMENT: 8bda08ab65cfc49b 2 anl6ND4KbWNPqEwSK8VhU6wWsik The wildtype cross yielded 81 ± 7.5% viable spores, the pnu1Δ cross 82 ± 3%. As far as tested, no change of meiosis and recombination was detected in mutants abolishing the function of the Pnu1 (End1) nuclease. ------- COMMENT: 8bfda07133be8ba6 1 I+uPi2BRyTGy20ayVfzbAQTdBoQ fig 1 (double mutant with cyr1 is more sensitive) ------- COMMENT: 8bfda07133be8ba6 3 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: 8bfda07133be8ba6 4 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: 8bfda07133be8ba6 5 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 8bfda07133be8ba6 6 JzyBKOIiyuH2U/GwaoWxC6/fJhQ Fig S2 ------- COMMENT: 8bfda07133be8ba6 7 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 8bfda07133be8ba6 8 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 8bfda07133be8ba6 9 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 8bfda07133be8ba6 10 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 8bfda07133be8ba6 11 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 8bfda07133be8ba6 13 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 8bfda07133be8ba6 15 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig 4 ------- COMMENT: 8bfda07133be8ba6 18 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: 8bfda07133be8ba6 19 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 8bfda07133be8ba6 20 j2SG4+FGU82dUT//jcUUlN5XK/c figure S2 ------- COMMENT: 8bfda07133be8ba6 21 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: 8bfda07133be8ba6 22 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: 8bfda07133be8ba6 26 w0wKkAH6w7GsNsCMiOGd8acmq1c figure 2b ------- COMMENT: 8bfda07133be8ba6 33 2Jx3cRBZBqp5hXcI7Owyc+W4IiQ cgs1∆ partially rescues plb1∆ on KCl ------- COMMENT: 8bfda07133be8ba6 34 skPH1SSY074g/rtlF9d5IUgNtoQ Fig 4 rst2∆ partially rescues plb1∆ on KCl ------- COMMENT: 8bfda07133be8ba6 35 po3OFU5YGpgsUAm1Zw9vNbwv3yQ rst2∆ rescues pka1∆ on KCl ------- COMMENT: 8bfda07133be8ba6 36 pcLtb5mPm5SG2C/hd35asAVQIFo cgs1∆ partially rescues cyr1∆ plb1∆ on KCl ------- COMMENT: 8bfda07133be8ba6 37 pnMJB1L7hQCaCpux9xLzJjwu78E cgs1∆ rescues cyr1∆ plb1∆ on sorbitol ------- COMMENT: 8bfda07133be8ba6 38 uiHL8rKrYLOE6LMKbZQVeP9Cwc8 Fig.1 Overexpression of Pka1 restores the KCl-sensitive pheno- type of the plb1∆ strain. ------- COMMENT: 8bfda07133be8ba6 39 ZT/NAYvakEmN06B3uNsDomnH+4o Fig 1b ------- COMMENT: 8bfda07133be8ba6 41 8P+ZvqUYBBPbZdvlsyL5RLH3nLw rst2∆ partially rescues pka1∆ plb1∆ on KCl ------- COMMENT: 8bfda07133be8ba6 42 eIGTx8sVdMnLplVL0I6KEyAY4kk rst2∆ rescues pka1∆ plb1∆ on KCl ------- COMMENT: 8bfda07133be8ba6 43 P8IiPumJ1Tgj7VS8gPT+HaGw/aM (comment: CHECK glucose MM) ------- COMMENT: 8bfda07133be8ba6 44 P8IiPumJ1Tgj7VS8gPT+HaGw/aM (comment: CHECK glucose MM) ------- COMMENT: 8bfda07133be8ba6 48 WAmKs/pXCrQG0cYWYBiQdvpPTOQ (comment: CHECK low glucose MM) ------- COMMENT: 8bfda07133be8ba6 49 ecB1qBnav/ryxMjOANxiFdQN+1M (comment: observed Pka1-GFP) ------- COMMENT: 8bfda07133be8ba6 50 ecB1qBnav/ryxMjOANxiFdQN+1M (comment: observed Pka1-GFP) ------- COMMENT: 8bfda07133be8ba6 51 ecB1qBnav/ryxMjOANxiFdQN+1M (comment: observed Pka1-GFP) ------- COMMENT: 8bfda07133be8ba6 52 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig 5 ------- COMMENT: 8bfda07133be8ba6 53 ecB1qBnav/ryxMjOANxiFdQN+1M (comment: observed Pka1-GFP) ------- COMMENT: 8c12b3f9cea0dcc2 3 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig 2 ------- COMMENT: 8c12b3f9cea0dcc2 11 IDQW37ca8/Kez2NohNhhvt5xNLY At anaphase II, however, many of the Dma1-GFP signals did not accumulate at SPBs (Fig 2 B&C) ------- COMMENT: 8c12b3f9cea0dcc2 12 2ssFNJE05JceDZCrlAudQM6sB0g Fig S1 ------- COMMENT: 8c12b3f9cea0dcc2 13 2ssFNJE05JceDZCrlAudQM6sB0g Fig S1 ------- COMMENT: 8c12b3f9cea0dcc2 16 RcWbGtzQBVyY33V2BWA4btRE+WE the initiation of spore formation was delayed for 􏰃2 h com- pared with wild-type cells, and also the efficiency of spore formation was dramatically dropped with only 􏰃60% of cells containing spores (Figure 3B). ------- COMMENT: 8c12b3f9cea0dcc2 17 U0beSB0BiAPOCveXjYnwl7T0MGg Figure 3B, Figure 4B the initiation of spore formation was delayed for 􏰃2 h com- pared with wild-type cells, and also the efficiency of spore formation was dramatically dropped with only 􏰃60% of cells containing spores ------- COMMENT: 8c12b3f9cea0dcc2 18 wf185u4DRDkg7Sbpsn3gLe6+2NY Figure 4E ------- COMMENT: 8c12b3f9cea0dcc2 19 wf185u4DRDkg7Sbpsn3gLe6+2NY Figure 4E ------- COMMENT: 8c12b3f9cea0dcc2 20 Zym0fzc6BYtPvn/9f4RJ4XOeWK8 Supplemental Figure S3 ------- COMMENT: 8c12b3f9cea0dcc2 21 Zym0fzc6BYtPvn/9f4RJ4XOeWK8 Supplemental Figure S3 ------- COMMENT: 8c12b3f9cea0dcc2 22 ye8mMQXnG9BWZKuocjPSVgGhKAk several types of defects in FSM de- velopment in dma1􏰁 cells (Figure 6B). These defects roughly fell into three classes: (1) initially FSM formation was normal and appeared as sphere structure, but subse- quently it became smaller or collapsed (asterisks in Figure 6B); (2) crescent-shaped structures did not properly develop into cup-like structures (open arrows in Figure 6B); and (3) crescent-shaped structures broke into multiple GFP-Psy1- contaning structures which could not develop into round mature FSMs (arrows in Figure 6B). ------- COMMENT: 8c12b3f9cea0dcc2 23 Pnql0VTi+VGGG4+34HsGxY8dMN0 dma1􏰁 spg1-106 and dma1􏰁 mob1-1 cells were com- pletely unable to sporulate under conditions in which single spg1-106 or mob1-1 mutants were not apparently compro- mised for sporulation (Figure 8, A and B), suggesting that Dma1 might function in parallel with Spg1 and Mob1 in sporulation. ------- COMMENT: 8c12b3f9cea0dcc2 24 Pnql0VTi+VGGG4+34HsGxY8dMN0 dma1􏰁 spg1-106 and dma1􏰁 mob1-1 cells were com- pletely unable to sporulate under conditions in which single spg1-106 or mob1-1 mutants were not apparently compro- mised for sporulation (Figure 8, A and B), suggesting that Dma1 might function in parallel with Spg1 and Mob1 in sporulation. ------- COMMENT: 8c12b3f9cea0dcc2 25 Pnql0VTi+VGGG4+34HsGxY8dMN0 dma1􏰁 spg1-106 and dma1􏰁 mob1-1 cells were com- pletely unable to sporulate under conditions in which single spg1-106 or mob1-1 mutants were not apparently compro- mised for sporulation (Figure 8, A and B), suggesting that Dma1 might function in parallel with Spg1 and Mob1 in sporulation. ------- COMMENT: 8c288b3ac3dc79cb 1 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 8c288b3ac3dc79cb 4 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 8c288b3ac3dc79cb 5 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 8c288b3ac3dc79cb 6 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 8c288b3ac3dc79cb 7 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 8c288b3ac3dc79cb 8 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 8c288b3ac3dc79cb 9 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 8c288b3ac3dc79cb 10 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 8c288b3ac3dc79cb 11 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 8c288b3ac3dc79cb 12 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 8c288b3ac3dc79cb 13 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 8c288b3ac3dc79cb 15 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 8c288b3ac3dc79cb 16 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 8c288b3ac3dc79cb 17 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 8c288b3ac3dc79cb 18 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 8c288b3ac3dc79cb 19 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 8c288b3ac3dc79cb 20 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 8c288b3ac3dc79cb 21 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 8c288b3ac3dc79cb 22 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 8c288b3ac3dc79cb 23 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 8c288b3ac3dc79cb 24 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 8c288b3ac3dc79cb 25 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 8c288b3ac3dc79cb 26 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 8c288b3ac3dc79cb 27 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 8c288b3ac3dc79cb 28 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 8c288b3ac3dc79cb 29 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 8c288b3ac3dc79cb 30 cAHtE8rtqkXXrgd7/1YkIe5uSJQ Importantly, by using a strain expressing a mutant version of Cpc2 (R36D/K38E) with reduced ability to associate with ribosomes in vivo (22), we also showed that ribosome binding of Cpc2 is critical for proper control of cell size at the G2/M boundary (Fig. 4A). ------- COMMENT: 8c288b3ac3dc79cb 31 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 8c288b3ac3dc79cb 32 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 8c288b3ac3dc79cb 33 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 8c288b3ac3dc79cb 34 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 8c288b3ac3dc79cb 35 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 8c288b3ac3dc79cb 36 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 8c288b3ac3dc79cb 37 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 8c288b3ac3dc79cb 38 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: 8c288b3ac3dc79cb 39 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: 8c288b3ac3dc79cb 40 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 8c288b3ac3dc79cb 41 mp0M7zDIFggn53e2MCluP++FNgs Fig. 5E ------- COMMENT: 8c288b3ac3dc79cb 42 mp0M7zDIFggn53e2MCluP++FNgs Fig. 5E ------- COMMENT: 8c288b3ac3dc79cb 43 T44vR21/o+u/qtfx00leUPdjBlI Fig. 5G ------- COMMENT: 8c288b3ac3dc79cb 44 T44vR21/o+u/qtfx00leUPdjBlI Fig. 5G ------- COMMENT: 8c288b3ac3dc79cb 45 cLKKHHathQCT1JZTf0i7RHzTu8A Fig. 5F ------- COMMENT: 8c288b3ac3dc79cb 46 cLKKHHathQCT1JZTf0i7RHzTu8A Fig. 5F ------- COMMENT: 8c288b3ac3dc79cb 47 EAZqTgYe6YyrU1HgFAFYIqm8jSY As a whole, our findings suggest that both Cdr2-dependent and -independent mechanisms are responsible for the increased Wee1 levels and the G2/M defect in cpc2D cells. ------- COMMENT: 8c5b305baa755d41 12 rqVjxfx6Iw222zYedqXZmN+9KNA (comment: CHECK Not affected by short-term actin cytoskeleton depolymerization by Latrunculin A) ------- COMMENT: 8c5b305baa755d41 13 /3y6sTuHoe5gqW7ihx9XpGtuVlQ Decreased levels of Cdc42 and Cdc42-GTP ------- COMMENT: 8c5b305baa755d41 14 Ked+VaDnqypbim7XaCEp8hY0faA Decreased levels of Cdc42 and Cdc42-GTP (CRIB) ------- COMMENT: 8c5b305baa755d41 15 uqZZje9wt5Rn92a3NBu7zHBjeeA Normal levels of Cdc42 and Cdc42-GTP (CRIB) ------- COMMENT: 8c5b305baa755d41 16 MydYyQlV2js4lcsuI4iK3ajWjy4 Increased levels of Cdc42 and Cdc42-GTP (CRIB) ------- COMMENT: 8c5b305baa755d41 17 uqZZje9wt5Rn92a3NBu7zHBjeeA Normal levels of Cdc42 and Cdc42-GTP (CRIB) ------- COMMENT: 8c5b305baa755d41 36 ekZaxXYUjXGgDy5m+Y6qqFi++9A (comment: Internally tagged functional allele, allowing live-imaging of Cdc42) ------- COMMENT: 8c5b305baa755d41 37 MydYyQlV2js4lcsuI4iK3ajWjy4 Increased levels of Cdc42 and Cdc42-GTP (CRIB) ------- COMMENT: 8c5b305baa755d41 55 uZEnkcUjV1amMdCOKina8lEKVyg (comment: CHECK more severe in presence of LatA) ------- COMMENT: 8c6ccb0ae9834279 73 YgW8BlVnHpUjpipMskXCZ+8euMY (comment: CHECK fragmented) ------- COMMENT: 8c6ccb0ae9834279 106 YgW8BlVnHpUjpipMskXCZ+8euMY (comment: CHECK fragmented) ------- COMMENT: 8c6ccb0ae9834279 108 YgW8BlVnHpUjpipMskXCZ+8euMY (comment: CHECK fragmented) ------- COMMENT: 8c6ccb0ae9834279 110 YgW8BlVnHpUjpipMskXCZ+8euMY (comment: CHECK fragmented) ------- COMMENT: 8c6ccb0ae9834279 112 YgW8BlVnHpUjpipMskXCZ+8euMY (comment: CHECK fragmented) ------- COMMENT: 8c6ccb0ae9834279 114 YgW8BlVnHpUjpipMskXCZ+8euMY (comment: CHECK fragmented) ------- COMMENT: 8c6ccb0ae9834279 115 YgW8BlVnHpUjpipMskXCZ+8euMY (comment: CHECK fragmented) ------- COMMENT: 8c6ccb0ae9834279 116 YgW8BlVnHpUjpipMskXCZ+8euMY (comment: CHECK fragmented) ------- COMMENT: 8c6ccb0ae9834279 117 YgW8BlVnHpUjpipMskXCZ+8euMY (comment: CHECK fragmented) ------- COMMENT: 8c6ccb0ae9834279 118 YgW8BlVnHpUjpipMskXCZ+8euMY (comment: CHECK fragmented) ------- COMMENT: 8c6ccb0ae9834279 120 YgW8BlVnHpUjpipMskXCZ+8euMY (comment: CHECK fragmented) ------- COMMENT: 8c6ccb0ae9834279 135 YgW8BlVnHpUjpipMskXCZ+8euMY (comment: CHECK fragmented) ------- COMMENT: 8c6ccb0ae9834279 137 YgW8BlVnHpUjpipMskXCZ+8euMY (comment: CHECK fragmented) ------- COMMENT: 8c6d16a0b6951d6d 1 p+ILnRrT1T+pEFFpgZaGcdFiAxk GST protein purified from cultures under each condition revealed the phosphorylation of Thr7, Ser22, Ser61, Thr63, and Thr69 of Ecl1 protein in the nutrient-rich environment (Figure 1d; Figure S1). Under conditions of sulfur or metal starvation,...... but those of Thr7 and Ser22 (situated near the N-terminus) decreased. ------- COMMENT: 8c6d16a0b6951d6d 2 p+ILnRrT1T+pEFFpgZaGcdFiAxk GST protein purified from cultures under each condition revealed the phosphorylation of Thr7, Ser22, Ser61, Thr63, and Thr69 of Ecl1 protein in the nutrient-rich environment (Figure 1d; Figure S1). Under conditions of sulfur or metal starvation,...... but those of Thr7 and Ser22 (situated near the N-terminus) decreased. ------- COMMENT: 8c6d16a0b6951d6d 6 Lm3GfvnNrmqZQ1eTr1pmG0jonNY Furthermore, the overexpression of the Ecl1 fusion protein using this plasmid led to CLS extension, suggesting that the protein was functional (Figure 1c). ------- COMMENT: 8c6d16a0b6951d6d 7 WdZLLxfaoUI9O759ISTV5h8t124 In addition, Ecl1 fusion protein overex- pression slowed the growth rate, similar to a previous report of Ecl1- overexpression (Ohtsuka et al., 2024a) (Figure 1c). ------- COMMENT: 8c6d16a0b6951d6d 8 oW7svT6ilDRBqH/8FRSxX/VPlqo The pulled-down Ecl1-GST protein was collected along with the Mip1-HA protein, suggesting a physi- cal interaction between Ecl1 and Mip1. Additionally, immunoprecip- itation with HA antibody resulted in the coprecipitation of Ecl1-GST with Mip1-HA (Figure 5c). ------- COMMENT: 8c6d16a0b6951d6d 9 X/bikvOEjrNQ8D0XA38aIj24gkU Furthermore, the pulled-down Ecl1-GST protein was also collected along with the Wat1-GFP-HA protein, suggesting a physicinteraction between Ecl1 and Wat1 (Figure 5d) ------- COMMENT: 8c6d16a0b6951d6d 10 c4dC02V8GyrP19KaGLXvAov1+X4 When Δecls cells were cultured in EMM, mating did not occur, not even in the stationary phase, but this defect was restored by the expression of wild-type Ecl1 or Ecl1-AA proteins by plasmids (Figure 2b). ------- COMMENT: 8c6d16a0b6951d6d 11 MtgZ+wmUb9BfqQr0IV0M1OPcbaQ This suggested that the Ecl1-AA mutant protein activity was comparable to that of the wild-type Ecl1 protein. Meanwhile, when Ecl1-DD was expressed, the recovery was low, and the degree of recovery was significantly lower than that of Ecl1 and Ecl1-AA. This suggested that the activity or stability of the Ecl1-DD mutant protein, which mimics the phosphorylation of Thr7 and Ser22, was reduced compared with the wild-type Ecl1 protein. (comment: (vw: there was no WT assay, but compared to WT in the same background)) ------- COMMENT: 8c6d16a0b6951d6d 12 MtgZ+wmUb9BfqQr0IV0M1OPcbaQ This suggested that the Ecl1-AA mutant protein activity was comparable to that of the wild-type Ecl1 protein. Meanwhile, when Ecl1-DD was expressed, the recovery was low, and the degree of recovery was significantly lower than that of Ecl1 and Ecl1-AA. This suggested that the activity or stability of the Ecl1-DD mutant protein, which mimics the phosphorylation of Thr7 and Ser22, was reduced compared with the wild-type Ecl1 protein. (comment: (vw: there was no WT assay, but compared to WT in the same background)) ------- COMMENT: 8c6d16a0b6951d6d 13 WdZLLxfaoUI9O759ISTV5h8t124 In addition, Ecl1 fusion protein overex- pression slowed the growth rate, similar to a previous report of Ecl1- overexpression (Ohtsuka et al., 2024a) (Figure 1c). ------- COMMENT: 8c6d16a0b6951d6d 14 S1qtRGsPRyyQfftvZyHwLMx77uo The number of cells in the G1 phase was considerably reduced in cells harboring pE- cl1-DD (Figure 2e).. ------- COMMENT: 8c6d16a0b6951d6d 15 4GmHFug5IXv5kt8ovWwESQa89JY First, we investigated the conjugation rate in the stationary phase. Overexpression of wild-type Ecl1 and Ecl1-22D, but not the empty vector or Ecl1-7D, rescued the conjugation defect in the sta- tionary phase of Δecls cells (Figure 3b), suggesting a significant loss of activity in Ecl1-7D. ------- COMMENT: 8c6d16a0b6951d6d 16 MtgZ+wmUb9BfqQr0IV0M1OPcbaQ This suggested that the Ecl1-AA mutant protein activity was comparable to that of the wild-type Ecl1 protein. Meanwhile, when Ecl1-DD was expressed, the recovery was low, and the degree of recovery was significantly lower than that of Ecl1 and Ecl1-AA. This suggested that the activity or stability of the Ecl1-DD mutant protein, which mimics the phosphorylation of Thr7 and Ser22, was reduced compared with the wild-type Ecl1 protein. (comment: (vw: there was no WT assay, but compared to WT in the same background)) ------- COMMENT: 8c6d16a0b6951d6d 17 VMjBxRrpHKY/54Z++5Dl9l2S6ws To investigate this, we observed the intracellular localization of the Ecl1-GFP fusion protein (Figure 4a). Regardless of mutations at Thr7 and Ser22, the Ecl1-GFP fusion pro- tein was mainly localized in the nucleus during logarithmic growth and was also observed in the cytoplasm. ------- COMMENT: 8c6d16a0b6951d6d 18 VMjBxRrpHKY/54Z++5Dl9l2S6ws To investigate this, we observed the intracellular localization of the Ecl1-GFP fusion protein (Figure 4a). Regardless of mutations at Thr7 and Ser22, the Ecl1-GFP fusion pro- tein was mainly localized in the nucleus during logarithmic growth and was also observed in the cytoplasm. ------- COMMENT: 8c6d16a0b6951d6d 19 CdJj4Z0LgBt6o8IpWAsTmr5eT1w This suggested that phosphoryla- tion of the N-terminus of Ecl1 was unlikely to significantly affect the intracellular localization of Ecl1. ------- COMMENT: 8c6d16a0b6951d6d 20 CdJj4Z0LgBt6o8IpWAsTmr5eT1w This suggested that phosphoryla- tion of the N-terminus of Ecl1 was unlikely to significantly affect the intracellular localization of Ecl1. ------- COMMENT: 8c6d16a0b6951d6d 21 568tXGJtK0IjxZ/FVI92y+ketVc TORC1 activity was reported to decrease in an ecl gene-dependent manner under conditions of sulfur or phosphate starvation (Ohtsuka et al., 2022a, 2023c) (Figure 5a). ------- COMMENT: 8c6d16a0b6951d6d 22 dT5R+XauUiuGOQYdkDGTT1Bju7U Ecl1, which physically interacts with Mip1, reduces TORC1 activity regardless of Thr7 mutation ------- COMMENT: 8c6d16a0b6951d6d 23 M02rCkrwkCrLytlSjX5fo90UiC8 Furthermore, the Ecl1-overexpression reduced TORC1 activity regardless of the pres- ence or absence of sulfur (Figure 5e,f). In other words, even during the logarithmic growth phase, when Thr7 is phosphorylated and Ecl function is suppressed, Ecl1 overexpression sufficiently reduces TORC1 activity. These results indicated that phosphorylation of the N-terminus of Ecl1 had little effect on TORC1 repression and that the expression of Ecl1, rather than starvation conditions, was im- portant for TORC1 repression. ------- COMMENT: 8c6d16a0b6951d6d 24 M02rCkrwkCrLytlSjX5fo90UiC8 Furthermore, the Ecl1-overexpression reduced TORC1 activity regardless of the pres- ence or absence of sulfur (Figure 5e,f). In other words, even during the logarithmic growth phase, when Thr7 is phosphorylated and Ecl function is suppressed, Ecl1 overexpression sufficiently reduces TORC1 activity. These results indicated that phosphorylation of the N-terminus of Ecl1 had little effect on TORC1 repression and that the expression of Ecl1, rather than starvation conditions, was im- portant for TORC1 repression. ------- COMMENT: 8c6d16a0b6951d6d 25 ZzIx1d/OolTmGXwo3UyX9DqQogo We investigated the effect of phosphorylation mutations of Ecl1 on the binding of TORC1 subunit Mip1 to Ecl1 (Figure 5i). Mip1-HA protein did not coprecipitate with GFP but did coprecip- itate wild-type Ecl1 and with Ecl1 proteins with mutations at Thr7 or Ser22. ------- COMMENT: 8c6d16a0b6951d6d 26 ZzIx1d/OolTmGXwo3UyX9DqQogo We investigated the effect of phosphorylation mutations of Ecl1 on the binding of TORC1 subunit Mip1 to Ecl1 (Figure 5i). Mip1-HA protein did not coprecipitate with GFP but did coprecip- itate wild-type Ecl1 and with Ecl1 proteins with mutations at Thr7 or Ser22. ------- COMMENT: 8c6d16a0b6951d6d 27 G/33szqC87uj2ALezGG32KRa2SI If CLS extension by Ecl1 is mediated by the same pathway as TORC1, no additive CLS extension would be observed if Ecl1 was overexpressed in a TORC1 subunit–defi- cient cell. Although wat1+ deletion or ecl1+ overexpression extended the CLS, overexpression of ecl1+ in the wat1-deficient cells did not result in further CLS extension, suggesting that Ecl1 and Wat1 function in CLS extension through the same path- way. ------- COMMENT: 8c6d16a0b6951d6d 28 G/33szqC87uj2ALezGG32KRa2SI If CLS extension by Ecl1 is mediated by the same pathway as TORC1, no additive CLS extension would be observed if Ecl1 was overexpressed in a TORC1 subunit–defi- cient cell. Although wat1+ deletion or ecl1+ overexpression extended the CLS, overexpression of ecl1+ in the wat1-deficient cells did not result in further CLS extension, suggesting that Ecl1 and Wat1 function in CLS extension through the same path- way. ------- COMMENT: 8c6d16a0b6951d6d 29 edPQqJIQYU1WC2oTxJyIgWuenRk If CLS extension by Ecl1 is mediated by the same pathway as TORC1, no additive CLS extension would be observed if Ecl1 was overexpressed in a TORC1 subunit–defi- cient cell. Although wat1+ deletion or ecl1+ overexpression extended the CLS, overexpression of ecl1+ in the wat1-deficient cells did not result in further CLS extension, suggesting that Ecl1 and Wat1 function in CLS extension through the same path- way. (However, because Wat1 is a component of TORC1 and TORC2 (Ahamad et al., 2018), further experiments are required to clarify whether Ecl1-induced CLS extension is exclusively mediated by TORC1) ------- COMMENT: 8c76b5840b281ac1 3 5qayz6Up7bm2Kyyp5WDvKCH9lPs (Fig. S1C) ------- COMMENT: 8c76b5840b281ac1 4 eb0t0w6QMb+FNn1f7AA3v1zc5k8 (Fig. 1F, Fig. S1G and Fig. 2C) ------- COMMENT: 8c76b5840b281ac1 6 38oJljKulSMlRPNwWoNbzCEOlHM (Fig. 4G) ------- COMMENT: 8c76b5840b281ac1 7 lzDpBhI+e1KR8hAG7s00IgdEh2I (Fig. 5A) ------- COMMENT: 8c76b5840b281ac1 8 kb7NOBRiqlmzY3vQrPFeR5NkglI (Fig. 7C) ------- COMMENT: 8c76b5840b281ac1 9 qgy2UtS49Rc8vkPrVMir2qiYd3M (Fig. 5F) ------- COMMENT: 8c76b5840b281ac1 10 BlZAYgg6W/+/O+S+HMuoElV4g98 (Fig. S6A) ------- COMMENT: 8c76b5840b281ac1 11 BlZAYgg6W/+/O+S+HMuoElV4g98 (Fig. S6A) ------- COMMENT: 8c76b5840b281ac1 12 bIIKqoJN5UwmvfrVyk8af1jehAo (Fig. S1K) ------- COMMENT: 8c76b5840b281ac1 13 cSAJ7EaUAvs+Q7QOjqSCJdTTlcs (Fig. 1D and E) ------- COMMENT: 8c76b5840b281ac1 14 QbxgMRvUU7IU0A828w/EYemDwWI (Fig. S1E and F) ------- COMMENT: 8c76b5840b281ac1 15 HfCxJCcyz5xKGwTBvEL3WRxBnVw (Fig. 4D and E) ------- COMMENT: 8c76b5840b281ac1 16 WryGMgYSnchRCvE8BK+aNVipgRI (Fig. 5B, Fig. S6C) ------- COMMENT: 8c76b5840b281ac1 17 KLOzvR5EIb1BMigGZXVDVLWmsW0 (Fig. S7B) ------- COMMENT: 8c76b5840b281ac1 18 mHHK047Ybq4WyRgqcXmo6GlF13I (Fig. S6F) ------- COMMENT: 8c76b5840b281ac1 19 SamTvRZvEvQIrgSzuqc8al8jR1s (Fig. S6B) ------- COMMENT: 8c76b5840b281ac1 20 SamTvRZvEvQIrgSzuqc8al8jR1s (Fig. S6B) ------- COMMENT: 8c76b5840b281ac1 21 KJ0O0ViKt6nyQ8qzrjrgosQi9K8 (Fig. S1I and J) ------- COMMENT: 8c76b5840b281ac1 22 cSAJ7EaUAvs+Q7QOjqSCJdTTlcs (Fig. 1D and E) ------- COMMENT: 8c76b5840b281ac1 23 xqjTztL6qGqaPgp6WgcG+iOomGI (Fig. S1B) ------- COMMENT: 8c76b5840b281ac1 24 bLmE8UPDTdmI/f/9a2LtNFsl3Z8 (Fig. S1G) ------- COMMENT: 8c76b5840b281ac1 25 bLmE8UPDTdmI/f/9a2LtNFsl3Z8 (Fig. S1G) ------- COMMENT: 8c76b5840b281ac1 26 bnUafqclLDStdQTkMGrue+BNj6k (Fig. S2B) ------- COMMENT: 8c76b5840b281ac1 27 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: 8c76b5840b281ac1 28 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: 8c76b5840b281ac1 29 ojGoCV7tkFuQq8EQLxws0hw4rK4 (Fig. S2D) ------- COMMENT: 8c76b5840b281ac1 30 gA4P48uKuk6ti5ukTRuiFvXo/Zw (Fig. S2C) ------- COMMENT: 8c76b5840b281ac1 31 mb04tZ6Um34qKdq5nQlfpYSAjoQ (Fig. S2F) ------- COMMENT: 8c76b5840b281ac1 32 mb04tZ6Um34qKdq5nQlfpYSAjoQ (Fig. S2F) ------- COMMENT: 8c76b5840b281ac1 33 mb04tZ6Um34qKdq5nQlfpYSAjoQ (Fig. S2F) ------- COMMENT: 8c76b5840b281ac1 34 mb04tZ6Um34qKdq5nQlfpYSAjoQ (Fig. S2F) ------- COMMENT: 8c76b5840b281ac1 35 mb04tZ6Um34qKdq5nQlfpYSAjoQ (Fig. S2F) ------- COMMENT: 8c76b5840b281ac1 36 mb04tZ6Um34qKdq5nQlfpYSAjoQ (Fig. S2F) ------- COMMENT: 8c76b5840b281ac1 37 mb04tZ6Um34qKdq5nQlfpYSAjoQ (Fig. S2F) ------- COMMENT: 8c76b5840b281ac1 38 mb04tZ6Um34qKdq5nQlfpYSAjoQ (Fig. S2F) ------- COMMENT: 8c76b5840b281ac1 39 mb04tZ6Um34qKdq5nQlfpYSAjoQ (Fig. S2F) ------- COMMENT: 8c76b5840b281ac1 40 mb04tZ6Um34qKdq5nQlfpYSAjoQ (Fig. S2F) ------- COMMENT: 8c76b5840b281ac1 41 mb04tZ6Um34qKdq5nQlfpYSAjoQ (Fig. S2F) ------- COMMENT: 8c76b5840b281ac1 42 mb04tZ6Um34qKdq5nQlfpYSAjoQ (Fig. S2F) ------- COMMENT: 8c76b5840b281ac1 43 mb04tZ6Um34qKdq5nQlfpYSAjoQ (Fig. S2F) ------- COMMENT: 8c76b5840b281ac1 44 caisk3ZmrdjW+p2nhfP0pw86f5c (Fig. S4) ------- COMMENT: 8c76b5840b281ac1 45 1j0rRUX0Bo3jZuDiINhW0c1EQck (Fig. 3, Fig. S3) ------- COMMENT: 8c76b5840b281ac1 46 WD3kRd80SyyYyGSzDepPv0TldzQ (Fig. 3G) ------- COMMENT: 8c76b5840b281ac1 47 PEijj9MIrNSBSecC4MlXqO1v4Zk (Fig. 3) ------- COMMENT: 8c76b5840b281ac1 48 caisk3ZmrdjW+p2nhfP0pw86f5c (Fig. S4) ------- COMMENT: 8c76b5840b281ac1 50 L84RMYxcmJK2Zj7t0R82RKoWDok (Fig. S6C) ------- COMMENT: 8c76b5840b281ac1 51 HfCxJCcyz5xKGwTBvEL3WRxBnVw (Fig. 4D and E) ------- COMMENT: 8c76b5840b281ac1 52 5hBvcfhVbME7Bc0BRA9dbyTF2rc (Fig. S7D) ------- COMMENT: 8c76b5840b281ac1 53 5hBvcfhVbME7Bc0BRA9dbyTF2rc (Fig. S7D) ------- COMMENT: 8c798b5961e500db 2 7Fq0aCT+RuDSs6eQ9msUN1P+wDo Only the unphosphorylated form of Cdc2 binds to Chk1. ------- COMMENT: 8c798b5961e500db 3 oDfHcsu/wER8SANtOxlEZZH4G3Q Chk1 binds to the unphosphorylated form of Cdc2 kinase ------- COMMENT: 8c798b5961e500db 4 nU+ijMLmAYB/6hyQREqI3ehpkNo Cdc13 (cyclin B) associates with the highly phosphorylated, but not with the medium phosphorylated form of Cdc2. It binds also to the unphosphorylated form of Cdc2 to which also Chk1 binds. ------- COMMENT: 8c798b5961e500db 5 oDfHcsu/wER8SANtOxlEZZH4G3Q Chk1 binds to the unphosphorylated form of Cdc2 kinase ------- COMMENT: 8c798b5961e500db 6 oDfHcsu/wER8SANtOxlEZZH4G3Q Chk1 binds to the unphosphorylated form of Cdc2 kinase ------- COMMENT: 8c798b5961e500db 19 tTpttj0CtGxZ8e0Ue4CkyqXmOsU modified forms of Cdc2 present differ from wild type ------- COMMENT: 8c798b5961e500db 20 LprYJbn1eGXCSjIQEQM9OJ6koQs modified forms of Cdc2 present differ from wild type, but are same as in cdc2-1w alone ------- COMMENT: 8c798b5961e500db 21 LprYJbn1eGXCSjIQEQM9OJ6koQs modified forms of Cdc2 present differ from wild type, but are same as in cdc2-1w alone ------- COMMENT: 8c798b5961e500db 22 Gyck+QxdlxgSKLIXGpXf3Ymhq4U modified forms of Cdc2 differ from both wild type and cdc2-1w alone ------- COMMENT: 8c7b04a443ae9d43 1 xSqw2JTQELuUd2X/MyKyxDCIJU8 In vitro RNA helicase activity using recombinant protein encoded by the helicase domain of Prp16 ------- COMMENT: 8c7b04a443ae9d43 2 mRSSZP/b/Dp8kA+QAjgBj4CrWlY Required for the splicing of several genome-wide transcripts. Inferred from transcriptome sequencing of the mutant strain prp16F528S. Splicing defects in transcripts validated by RT-PCR assays. ------- COMMENT: 8c7b04a443ae9d43 8 3GPtV+MNaYbzngMZ65pi3AMp26I (comment: CHECK synthetic sick interaction between prp16F528S and prp8-1) ------- COMMENT: 8c7b04a443ae9d43 9 AOo33gkuNtSQeavEzlVpTKQwcLc (comment: CHECK synthetic lethal interaction between prp16F528S and dbr1∆) ------- COMMENT: 8c7b04a443ae9d43 10 cW01MPlJQTlD8C+cvrHG3aLYAz4 (comment: CHECK cold sensitivity of prp16F528S rescued by cwf10-1) ------- COMMENT: 8c7b04a443ae9d43 11 PSXw0LetrQj6+jYxiAChx8sts8U (comment: CHECK splicing defect of candidate transcripts in prp16F528S partially rescued by overexpression of prp22) ------- COMMENT: 8c7b04a443ae9d43 12 g5uQB/GrSWlnT7SsuCAsQRWdgXQ (comment: Required for splicing of introns with strong 5' splice site - U6 snRNA and branch site - U2 snRNA interactions) ------- COMMENT: 8c7b04a443ae9d43 13 oXrahwH/EUTq3pU54FPDXMsc++Y fig 1 ------- COMMENT: 8c7b04a443ae9d43 14 oXrahwH/EUTq3pU54FPDXMsc++Y fig 1 ------- COMMENT: 8c7b04a443ae9d43 15 oXrahwH/EUTq3pU54FPDXMsc++Y fig 1 ------- COMMENT: 8c7b04a443ae9d43 16 oXrahwH/EUTq3pU54FPDXMsc++Y fig 1 ------- COMMENT: 8c7b04a443ae9d43 17 oXrahwH/EUTq3pU54FPDXMsc++Y fig 1 ------- COMMENT: 8c7b04a443ae9d43 18 U/PvCZ3/sYCTc+VkXd+IsT2uQcI fig 1b ------- COMMENT: 8c7b04a443ae9d43 19 U/PvCZ3/sYCTc+VkXd+IsT2uQcI fig 1b ------- COMMENT: 8c7b04a443ae9d43 20 v1TpBbz/Qz9zVqySIhVgbedkM1A fig1b - for dga1 normal splicing of inton 3, abnormal intron 2, Fig. S4 ------- COMMENT: 8c7b04a443ae9d43 21 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 8c7b04a443ae9d43 22 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 8c7b04a443ae9d43 23 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 8c7b04a443ae9d43 24 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 8c7b04a443ae9d43 25 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 8c7b04a443ae9d43 27 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 8c7b04a443ae9d43 28 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 8c7b04a443ae9d43 29 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 8c7b04a443ae9d43 30 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: 8c7b04a443ae9d43 31 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: 8c7b04a443ae9d43 33 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: 8c7b04a443ae9d43 34 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: 8c7b04a443ae9d43 35 gXh5RXfN+qM+IuMhJ1DiFbf/lVU fig7 (comment: CHECK they say fragmented nucleus but they stained chromosomes, not nuclear envelope) ------- COMMENT: 8c7b04a443ae9d43 36 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: 8c7b04a443ae9d43 37 HWqAfzN+KmzBpOBhEC62KXrfunE figS6, 7 ------- COMMENT: 8c7b04a443ae9d43 38 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: 8c7b04a443ae9d43 40 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: 8c7b04a443ae9d43 41 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: 8c7b04a443ae9d43 42 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: 8c7b04a443ae9d43 43 xSqw2JTQELuUd2X/MyKyxDCIJU8 In vitro RNA helicase activity using recombinant protein encoded by the helicase domain of Prp16 ------- COMMENT: 8ca59104c61e0b02 1 RdYnsIukvKe4TVUPQY9FyQ4zmRc Fig1B At 25°C the permissive temperature rad3ts the checkpoint is active and cells expressing the mutant form of pREP4X cdc18 from the screen elongate ------- COMMENT: 8ca59104c61e0b02 2 4Rdp+fQ6GJPxymAjKkG3i4H0f+U Fig1C rad3ts cells over expressing the mutated pREP4X-cdc18 construct do not rereplicate at 25°C or 36°C (the permissive and non permissive temperatures for rad3ts). This is in contrast to Control cells expressing cdc18+ from pREP3X promoter at 32°C (Fig1D , Table 2) which don't activate the mitotic DNA checkpoint and undergo DNA re-replicatiom ------- COMMENT: 8ca59104c61e0b02 3 HlYUNgC0Ss7x61FYrUNMr8WEbZc Fig 2A cdc18T6A expression at 25°C causes cell elongation due to activation of the mitotic DNA replication checkpoint ( permissive temperature for rad3ts) ------- COMMENT: 8ca59104c61e0b02 4 qvb8QQ2bPGf63TksStdLN4qmycY Fig2B cells arrested due to activation of the rad3 checkpoint gene do not rereplicate ------- COMMENT: 8ca59104c61e0b02 5 wxcmAvzIkaHaNFbOb0UJlLtrtY8 Fig2 A,C cells arrested due to activation of the rad3 (permissive temperature) have normal nuclear to cell size ratio (NC ratio) compared to cdc25-22 at restrictive temp 2 hr ------- COMMENT: 8ca59104c61e0b02 6 gaw6etPnIStf9nB1LpC25ilgB8g Fig2D different to when pREP3X cdc18+ is over expressed in G2 block which show replicate intermediates and cells undergo re replication ------- COMMENT: 8ca59104c61e0b02 10 PXqQ8w7WZKoenOmS6L1/H8ouUb4 Fig4A ------- COMMENT: 8ca59104c61e0b02 12 Hg9Eu4qbBQzKQgDHIQdXmeKgbSI Fig4C At the permissive temperature 25*C rad3 is active but checkpoint cannot be activated in absence of chk1 ------- COMMENT: 8ca59104c61e0b02 13 b7sVO8oOdEGVVlrt5s5INXxeeU8 Fig4C At 25°C rad3 is active but checkpoint cannot be activated in absence of rad9 ------- COMMENT: 8ca59104c61e0b02 14 K22ngHURULMCyIM8NUaAEfoY26A Fig4C At 25°C rad3 is active but checkpoint cannot be activated in absence of rad17 ------- COMMENT: 8ca59104c61e0b02 15 LniqOQ0356FmAYepfz+OgPuYWZE Fig4C At 25°C rad3 is active but checkpoint cannot be activated in absence of rad1 ------- COMMENT: 8ca59104c61e0b02 16 hglOjxBsyVF0jI8KHkRUSifOSTU Fig4C At 25°C rad3 is active but checkpoint cannot be activated in absence of rad26 ------- COMMENT: 8ca59104c61e0b02 17 d6aQc1rHVAzRvL+Yh+mirGLc92Y Fig4C At 25°C rad3 is active but checkpoint cannot be activated in absence of crb2 ------- COMMENT: 8ca59104c61e0b02 19 lv4dFJcUrjYRBi1q0xtO8my8jAQ Fig4C at 25°C rad3ts is active and the checkpoint is activated in absence of cds1 ------- COMMENT: 8ca59104c61e0b02 20 SyocknQprYyhqoDrkWJAobftZ04 Fig4C at the permissive temperature rad3ts is active and the checkpoint is activated in absence of mrc1 ------- COMMENT: 8ca59104c61e0b02 21 VFkskau1qxV6L9SVWfIqbfw500g Fig 5A ------- COMMENT: 8ca59104c61e0b02 22 UGKLbMSoFrW9s5nNAMBghTw/TiA Fig 5B ------- COMMENT: 8ca59104c61e0b02 23 REqA86wgEckE1+po1IZQ8wC5RUg Fig5C ------- COMMENT: 8ca59104c61e0b02 24 REqA86wgEckE1+po1IZQ8wC5RUg Fig5C ------- COMMENT: 8ca59104c61e0b02 25 ROtrA44QfG8vGVjFfBul/AgxTYg Fig6A, B ------- COMMENT: 8ca59104c61e0b02 26 49/1/QWjRTngAYyAc5o/yFVlY2w Fig1B This is the mutant form of pREP4X cdc18 from the screen At 25°C rad3 checkpoint is active and cells elongate, At 36°C rad3 is inactive and cells do not elongate when cdc18+ is expressed from pREP4X promoter, ------- COMMENT: 8ca59104c61e0b02 27 iP3pkPBTDOTChEj2nZVx69lcDMY Fig 2A when cdc18TA6 is overexpressed at 36°C (non permissive temperature for rad3ts ) cells continue to grow and divide normally ( permissive temperature for cdc18TA6) ------- COMMENT: 8ca59104c61e0b02 28 VlnEBP561+VD/mf5rgCDZ/AK4v4 Fig 4C At 25°C rad3 is active but checkpoint cannot be activated in absence of hus1 ------- COMMENT: 8ca59104c61e0b02 29 cIf1t2Y8AyZrsKux7vFINo+t4Wo Fig3 and previous figs shows lack of re replication with moderate increase in cdc18 protein level ------- COMMENT: 8ca59104c61e0b02 30 cIf1t2Y8AyZrsKux7vFINo+t4Wo Fig3 and previous figs shows lack of re replication with moderate increase in cdc18 protein level ------- COMMENT: 8ca59104c61e0b02 31 6apakWTWbxsUEkE6cmbF5XBxv9k Fig 6C shows Chromosome III smear is present throughout the cell cycle ------- COMMENT: 8ca59104c61e0b02 32 DQirL21t4AjTBZSUysE0Wb+pN5I Fig7A, B, C after crossing out of cdc18TA6 chromosome size is stabilised and a single band is seen on PFGE which varies in size. If strains with larger Chr III are culture for <30 generations Chromosome III gradually reduces in size ------- COMMENT: 8ca59104c61e0b02 33 noQMLfPHCj6qZ6Df6FY3Pg1I3Gw Fig 8A, B ------- COMMENT: 8ca59104c61e0b02 34 fTaut3N1YxuZQ4hRn2yoaHHeNMQ Fig9A ------- COMMENT: 8ca59104c61e0b02 35 Mpf9sUECD7MFv+y7+oGh+/vxU3k Fig9B, C reb1D reduces amount inappropriate recombination at DNA repeats leading to a reduction in cell elongation during checkpoint activation ------- COMMENT: 8ca59104c61e0b02 36 B20onHCE0tl6NBcOSmlLCG0P1IE The western blot in Fig 3 actually shows the TAP tagged version but they use the two strains interchangeable so don't actually show data for this strain CCL9 ------- COMMENT: 8ca59104c61e0b02 37 xuov7m+xjCmceHrwp6vXqReVyX4 Fig3 (comment: CHECK This is the mutant form of pREP4X cdc18 from the screen) ------- COMMENT: 8ca59104c61e0b02 38 vfYrqv1hh+6uhPcIPDsYe0J4YHc Fig1B At 36°C the restrictive temperature for rad3ts the checkpoint is inactive and cells expressing the mutant form of pREP4X cdc18 from the screen do not elongate and are able to form colonies ------- COMMENT: 8ca59104c61e0b02 39 SgeHMqTp8ZBj7+8PmN9KHatob70 Fig 2A cells expressing cdc18T6A at 36°C do not elongate at the nonpermissive temperature for rad3ts due to inactivation of the mitotic DNA replication checkpoint ------- COMMENT: 8ca59104c61e0b02 41 0o03xRkqQwyHIEuII85ZMWBn9g8 Fig3 cdc18 expressed from pREP3X is at a high level. They argue that higher levels cdc18 lead to rereplication and lower levels lead to a rad3 dependent block but no rereplication ------- COMMENT: 8ca59104c61e0b02 43 AvYbqbdb/bl/xKp3vqDOvLOzFN0 Fig9B, C rad52D reduces amount inappropriate recombination at DNA repeats leading to a reduction in cell elongation during checkpoint activation ------- COMMENT: 8ca59104c61e0b02 44 4Rdp+fQ6GJPxymAjKkG3i4H0f+U Fig1C rad3ts cells over expressing the mutated pREP4X-cdc18 construct do not rereplicate at 25°C or 36°C (the permissive and non permissive temperatures for rad3ts). This is in contrast to Control cells expressing cdc18+ from pREP3X promoter at 32°C (Fig1D , Table 2) which don't activate the mitotic DNA checkpoint and undergo DNA re-replicatiom ------- COMMENT: 8ca59104c61e0b02 45 wxcmAvzIkaHaNFbOb0UJlLtrtY8 Fig2 A, C cells arrested due to activation of the rad3 (permissive temperature) have normal nuclear to cell size ratio (NC ratio) compared to cdc25-22 at restrictive temp 2 hr ------- COMMENT: 8ca59104c61e0b02 46 ROtrA44QfG8vGVjFfBul/AgxTYg Fig6A, B ------- COMMENT: 8cb6518bf2bf38dc 7 o4eSmlYoiGueN41DSPF64jz0Tdo Figure1G-H ------- COMMENT: 8cb6518bf2bf38dc 8 o4eSmlYoiGueN41DSPF64jz0Tdo Figure1G-H ------- COMMENT: 8cb6518bf2bf38dc 9 IrJs8uOP5XO9bP/l4OTqbhYX6qc Figure 1G-H ------- COMMENT: 8cb6518bf2bf38dc 10 o4eSmlYoiGueN41DSPF64jz0Tdo Figure1G-H ------- COMMENT: 8cef0a6f9aaae17e 12 ka+CEFcKVsJEK0VdR/KleNT7faM (comment: TOR kinase activity was measured using immunoprecipitated proteins) (Fig.2) ------- COMMENT: 8cef0a6f9aaae17e 17 LR5ITXFPegTrBh8JYTLf7X/yKRA Figure 4e ------- COMMENT: 8cef0a6f9aaae17e 20 vDxiz1txQQ29bqsbM4rQZGHcejg Figure 4a, b ------- COMMENT: 8cef0a6f9aaae17e 21 59tU52sNyni5zQsSsfF+Sv5GLtM Figure 4d ------- COMMENT: 8cef0a6f9aaae17e 24 preevNra+95j0Wsfhl0K+KSDRAs Figure 1a ------- COMMENT: 8cef0a6f9aaae17e 25 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 8cef0a6f9aaae17e 59 y/9QJLPFSO3OmLTxqZTTEGsQOF0 Figure 4d ------- COMMENT: 8cef0a6f9aaae17e 76 Y9aqecoJtgbjS/1NkSqFn7IX0Ik Figure 1a, 6a ------- COMMENT: 8cef0a6f9aaae17e 77 preevNra+95j0Wsfhl0K+KSDRAs Figure 1a ------- COMMENT: 8cef0a6f9aaae17e 78 preevNra+95j0Wsfhl0K+KSDRAs Figure 1a ------- COMMENT: 8cef0a6f9aaae17e 79 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 8cef0a6f9aaae17e 80 JyKnxZeJ5mGBsqVFTMpU7nIJMXo Figure 6 ------- COMMENT: 8cfa36fcddaa98fa 1 gZNo/xWKvKsHWWGOazkQEqseH2U (Fig. 1) ------- COMMENT: 8cfa36fcddaa98fa 2 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: 8cfa36fcddaa98fa 3 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: 8cfa36fcddaa98fa 4 p3x2dDTjQlS9jbO+IMHpHbED4nw (Fig. 1D) ------- COMMENT: 8cfa36fcddaa98fa 5 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: 8cfa36fcddaa98fa 6 38oJljKulSMlRPNwWoNbzCEOlHM (Fig. 4G) ------- COMMENT: 8cfa36fcddaa98fa 7 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 8cfa36fcddaa98fa 8 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 8cfa36fcddaa98fa 9 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 8cfa36fcddaa98fa 10 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 8cfa36fcddaa98fa 12 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 8cfa36fcddaa98fa 13 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 8cfa36fcddaa98fa 14 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 8cfa36fcddaa98fa 15 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 8cfa36fcddaa98fa 16 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: 8cfa36fcddaa98fa 17 5eJ/kC0YLArHAvSN9OYC+z2Nkxc (Fig. EV2) ------- COMMENT: 8cfa36fcddaa98fa 18 knNFrysPynL9jm8GAW0UBUJGzwI This analysis revealed that aal1 RNAs predominantly localize in the cytoplasm in multiple foci (Fig. 2B). ------- COMMENT: 8cfa36fcddaa98fa 87 3rMl5sm1L2+fy/l2guSBEzoj4b8 (Fig. 4A and C) ------- COMMENT: 8cfa36fcddaa98fa 88 5eLctGd5Yaxs629EV/9EPcMYv2w (Fig. 4B and C) ------- COMMENT: 8cfa36fcddaa98fa 89 5tEr19lZ889dGeKurZ925NdOZno (Fig. 4E and F) ------- COMMENT: 8cfa36fcddaa98fa 90 5tEr19lZ889dGeKurZ925NdOZno (Fig. 4E and F) ------- COMMENT: 8cfa36fcddaa98fa 91 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: 8cfa36fcddaa98fa 92 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: 8cfa36fcddaa98fa 93 qgy2UtS49Rc8vkPrVMir2qiYd3M (Fig. 5F) ------- COMMENT: 8cfa36fcddaa98fa 94 qgy2UtS49Rc8vkPrVMir2qiYd3M (Fig. 5F) ------- COMMENT: 8cfa36fcddaa98fa 95 kuRdbpnFrU9sjksgW9GQwEQ1424 (Fig. 5) ------- COMMENT: 8d0ac74703887de6 1 dUzWFB5TVNdJNGnwzpWMUv1usco interaction between full length Atg101 and N-terminal HORMA domain of Atg13 ------- COMMENT: 8d0ac74703887de6 2 Hih0ucyPplOUb2XAobW4VSz0V5E interaction between full length Atg17 and Atg13 C-terminal domain ------- COMMENT: 8d0ac74703887de6 3 xPjSsRzUeX9TSAZxNME42YSs5VQ interaction between C-terminal domains of Atg1 and Atg13 ------- COMMENT: 8d0ac74703887de6 4 HhkwtOGCSbk7Y3O2wlzRUW+jR9o weak interaction with C-terminal domain of Atg1 ------- COMMENT: 8d0ac74703887de6 6 UNphfuWjKYDIxGZcU419RfBLDLQ Based on negative stain analysis ------- COMMENT: 8d0ac74703887de6 7 Ntwrd88Pz+HCZljUiVZgCHLnfq8 fig 1D ------- COMMENT: 8d0ac74703887de6 8 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 8d0ac74703887de6 9 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 8d0ac74703887de6 10 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 8d0ac74703887de6 12 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 8d41506ec01aec5b 6 sLLAlSmB84/2gM6823jfAOf/cPc Figure 2, Table 2 ------- COMMENT: 8d41506ec01aec5b 9 88j8JKPLV2+OfoCnKPKiRAKBJx8 Figure 4B and D ------- COMMENT: 8d41506ec01aec5b 10 88j8JKPLV2+OfoCnKPKiRAKBJx8 Figure 4B and D ------- COMMENT: 8d41506ec01aec5b 21 WXP0U5MC7CFt29Wl+3GG8EhgFBQ (comment: arrested) ------- COMMENT: 8d41506ec01aec5b 23 WXP0U5MC7CFt29Wl+3GG8EhgFBQ (comment: arrested) ------- COMMENT: 8d41506ec01aec5b 25 WXP0U5MC7CFt29Wl+3GG8EhgFBQ (comment: arrested) ------- COMMENT: 8db0c2a727e8b74f 13 mYW/fLjn8O8WqoN/ssH7ZCMuRWk (comment: CHECK is this OK? its aseptate?) ------- COMMENT: 8ddb4e6192c755eb 1 uIC6LVCRmi+EMNxRz8nQg912LK4 Interestingly, we found that Psc3 is enriched at the heterochromatic locations containing Swi6, such as the silent mating-type locus and the centromeric repeats (Fig. 1b, c, wild type). ------- COMMENT: 8ddb4e6192c755eb 2 uIC6LVCRmi+EMNxRz8nQg912LK4 Interestingly, we found that Psc3 is enriched at the heterochromatic locations containing Swi6, such as the silent mating-type locus and the centromeric repeats (Fig. 1b, c, wild type). ------- COMMENT: 8ddb4e6192c755eb 3 uIC6LVCRmi+EMNxRz8nQg912LK4 Interestingly, we found that Psc3 is enriched at the heterochromatic locations containing Swi6, such as the silent mating-type locus and the centromeric repeats (Fig. 1b, c, wild type). ------- COMMENT: 8ddb4e6192c755eb 4 uIC6LVCRmi+EMNxRz8nQg912LK4 Interestingly, we found that Psc3 is enriched at the heterochromatic locations containing Swi6, such as the silent mating-type locus and the centromeric repeats (Fig. 1b, c, wild type). ------- COMMENT: 8ddb4e6192c755eb 5 eJi++8dijm8qM3lsy9kw0ksv5tE shown), and the association with otr and KR was much reduced (Fig. 1d). ------- COMMENT: 8ddb4e6192c755eb 6 Z7oCbgCq2hBWfqLXC0BFfz01Szc However, this mutation did not affect Swi6 localization at otr and KR (Fig. 1e). ------- COMMENT: 8ddb4e6192c755eb 7 eiViN9l0q5nMQxdz6yckeXSufLA We consistently observed that psc3-4T and the other psc3 mutant alleles had no detectable effect on the silencing of a marker gene inserted at either centromeric (otr1R::ura4+) repeats or in the silent mating-type (Kint2::ura4+) region (see Supplementary Information). ------- COMMENT: 8ddb4e6192c755eb 8 eiViN9l0q5nMQxdz6yckeXSufLA We consistently observed that psc3-4T and the other psc3 mutant alleles had no detectable effect on the silencing of a marker gene inserted at either centromeric (otr1R::ura4+) repeats or in the silent mating-type (Kint2::ura4+) region (see Supplementary Information). ------- COMMENT: 8ddb4e6192c755eb 9 yLok4IdLZ3BY8/k99VCphxiK7wY Psc3 localization was strikingly disrupted in the swi6∆ strain at both loci (Fig. 1b). ------- COMMENT: 8ddb4e6192c755eb 10 yLok4IdLZ3BY8/k99VCphxiK7wY Psc3 localization was strikingly disrupted in the swi6∆ strain at both loci (Fig. 1b). ------- COMMENT: 8ddb4e6192c755eb 11 yLok4IdLZ3BY8/k99VCphxiK7wY Psc3 localization was strikingly disrupted in the swi6∆ strain at both loci (Fig. 1b). ------- COMMENT: 8ddb4e6192c755eb 12 yLok4IdLZ3BY8/k99VCphxiK7wY Psc3 localization was strikingly disrupted in the swi6∆ strain at both loci (Fig. 1b). ------- COMMENT: 8ddb4e6192c755eb 13 YHyq59oYd+mZP9P/flQQJmkVc8I psc3-1T also displays the lagging- chromosome phenotype and mis-segregated the mini-chromosome Ch16 (ref. 17) at a higher rate than the wild type, mimicking the swi6∆ phenotype (Fig. 2a, b). ------- COMMENT: 8ddb4e6192c755eb 14 YHyq59oYd+mZP9P/flQQJmkVc8I psc3-1T also displays the lagging- chromosome phenotype and mis-segregated the mini-chromosome Ch16 (ref. 17) at a higher rate than the wild type, mimicking the swi6∆ phenotype (Fig. 2a, b). ------- COMMENT: 8ddb4e6192c755eb 15 occ4x+wdmfgPpNJZ+tW/imFWsEo (Fig. 2a, b). ------- COMMENT: 8ddb4e6192c755eb 16 occ4x+wdmfgPpNJZ+tW/imFWsEo (Fig. 2a, b). ------- COMMENT: 8ddb4e6192c755eb 19 cIb3Za6y+h8N9WcDRGQnYaHA5XQ Figure 2b ------- COMMENT: 8ddb4e6192c755eb 20 kzku/qTck5Fa3TgCekHaumeFV50 Moreover, the psc3+–GFP swi6∆ cells showed increased mini-chromosome loss and exhibited a high incidence of lagging chromosome and other chromosome segregation abnormalities (Fig. 2c) ------- COMMENT: 8ddb4e6192c755eb 21 L61uD+SLmlP3w0oapzTYCvtD4Po we found that cohesin mutants (psc3-1T, psc3-2T, psc3-4T and rad21-K1) pro- duced switching-defective colonies at a rate nearly 100-fold that of the wild type (Fig.3a,b). ------- COMMENT: 8ddb4e6192c755eb 22 L61uD+SLmlP3w0oapzTYCvtD4Po we found that cohesin mutants (psc3-1T, psc3-2T, psc3-4T and rad21-K1) pro- duced switching-defective colonies at a rate nearly 100-fold that of the wild type (Fig.3a,b). ------- COMMENT: 8ddb4e6192c755eb 23 L61uD+SLmlP3w0oapzTYCvtD4Po we found that cohesin mutants (psc3-1T, psc3-2T, psc3-4T and rad21-K1) pro- duced switching-defective colonies at a rate nearly 100-fold that of the wild type (Fig.3a,b). ------- COMMENT: 8ddb4e6192c755eb 24 L61uD+SLmlP3w0oapzTYCvtD4Po we found that cohesin mutants (psc3-1T, psc3-2T, psc3-4T and rad21-K1) pro- duced switching-defective colonies at a rate nearly 100-fold that of the wild type (Fig.3a,b). ------- COMMENT: 8ddb4e6192c755eb 25 1T6QwpBGXRo0+b4Y7gh/Ya53WIM (Fig. 3b, data not shown) ------- COMMENT: 8de8896718b55bd8 4 8mqvm35PuxahOa7ZaB/RMIWZ8hQ Together, these results indicate that Wsc1 clustering may be triggered by local surface compression, independently of puta- tive ‘‘trans’’ homotypic interactions between extracellular sensors from neighbor cells or general cell-to-cell signaling. ------- COMMENT: 8de8896718b55bd8 5 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 6 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 7 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 8 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 9 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 10 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 11 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 12 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 13 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 14 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 15 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 16 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 17 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 18 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 19 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 20 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 21 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 22 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 23 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 24 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 25 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 26 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: 8de8896718b55bd8 28 ML/5xDSQNr8V0IZOUNUHQPrStUE Remarkably, the Wsc1DCC-GFP lacking a large fraction of the cytoplasmic C-ter- minal tail, and thus presumably defective in downstream signal transduction was dispensable for clustering. This finding rein- forces the notion that Wsc1 clustering occurs independently of downstream CWI signaling. ------- COMMENT: 8de8896718b55bd8 29 FsayeJDBWcKgaC6O8ECYodhJm3k In sharp contrast, Wsc1DSTR-GFP, Wsc1DWSC-GFP and Wsc1DSTRDWSC-GFP cells were completely devoid of any clustering phenotype, with an enrichment at con- tacts close to $2 (Figures 5D, 5E, and S2F–S2H). ------- COMMENT: 8de8896718b55bd8 30 FsayeJDBWcKgaC6O8ECYodhJm3k In sharp contrast, Wsc1DSTR-GFP, Wsc1DWSC-GFP and Wsc1DSTRDWSC-GFP cells were completely devoid of any clustering phenotype, with an enrichment at con- tacts close to $2 (Figures 5D, 5E, and S2F–S2H). ------- COMMENT: 8de8896718b55bd8 31 FsayeJDBWcKgaC6O8ECYodhJm3k In sharp contrast, Wsc1DSTR-GFP, Wsc1DWSC-GFP and Wsc1DSTRDWSC-GFP cells were completely devoid of any clustering phenotype, with an enrichment at con- tacts close to $2 (Figures 5D, 5E, and S2F–S2H). ------- COMMENT: 8de8896718b55bd8 32 NU4wFKiLJ1XWezbHUiVTnlbbrJ8 increased lateral diffusion in membrane. Finally, Wsc1DWSC-GFP and Wsc1DSTR-GFP, which are incapable of clustering, exhibited much smaller half-times—closer to that of mCherry-Psy1 (Figures 6A, 6B, and S6A). ------- COMMENT: 8de8896718b55bd8 38 0yOcVWGgG3PQ2pLgWzhEy+hFCXQ n these videos, we did not detect any lysis in WT red cells, but a high incidence of dying wsc1DCC-GFP of $26% ------- COMMENT: 8de8896718b55bd8 39 j4o1ge/GVHC9Si+5VC3W0BTvR2w In microchannels, where clusters form at high frequency, due to larger and more frequent compressive mechanical stresses onto the CWs, the survival behavior was markedly different. First, wsc1D cells exhibited a much higher yield of death of $28%. Second, all alleles exhibited a high yield of death around $25% including wsc1DWSC ------- COMMENT: 8de8896718b55bd8 44 Kvg+8vWgab/FC+nMcbDDqQ8puxU (comment: CHECK (vw: 25% cell death)) ------- COMMENT: 8de8896718b55bd8 45 NU4wFKiLJ1XWezbHUiVTnlbbrJ8 increased lateral diffusion in membrane. Finally, Wsc1DWSC-GFP and Wsc1DSTR-GFP, which are incapable of clustering, exhibited much smaller half-times—closer to that of mCherry-Psy1 (Figures 6A, 6B, and S6A). ------- COMMENT: 8df35cf35591f150 21 bpmwJ6bOZcTf+xmp0EC3BzyMYvQ (comment: IPI and IMP evidence) ------- COMMENT: 8e81367dbe1eb41c 1 Npj+BCAIQn3pYGuZcL4H27F4s6o Fig. 2D ------- COMMENT: 8e81367dbe1eb41c 2 d95o2uzYI7q7tY7bHI4U1xBug7s Fig. 3 ------- COMMENT: 8e81367dbe1eb41c 4 G2RTiSRzpGfQc3LUXrBavCAxZHo Fig. 4 ------- COMMENT: 8e81367dbe1eb41c 5 G2RTiSRzpGfQc3LUXrBavCAxZHo Fig. 4 ------- COMMENT: 8e81367dbe1eb41c 6 G2RTiSRzpGfQc3LUXrBavCAxZHo Fig. 4 ------- COMMENT: 8e81367dbe1eb41c 7 G2RTiSRzpGfQc3LUXrBavCAxZHo Fig. 4 ------- COMMENT: 8e81367dbe1eb41c 8 G2RTiSRzpGfQc3LUXrBavCAxZHo Fig. 4 ------- COMMENT: 8e81367dbe1eb41c 9 G2RTiSRzpGfQc3LUXrBavCAxZHo Fig. 4 ------- COMMENT: 8e81367dbe1eb41c 10 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: 8e81367dbe1eb41c 11 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: 8e81367dbe1eb41c 12 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: 8eba150e69a624ea 1 goc4pfsV8r9ID4Yr5CXkBWFf0o0 Figure 3D; (comment: CHECK endogenous atf1+ was deleted) ------- COMMENT: 8eba150e69a624ea 2 uXx2JTMai7qkEzYv9hg7P4Kddy0 Figure 3- S2A, B ------- COMMENT: 8eba150e69a624ea 3 Qo+Oo6xZUniwOPj5J6CsTWE3FsM Figure 5B; Notably, cells expressing two copies, but not one copy, of wis1-DD showed severe gene silencing defects (Figure 5B and Figure 5—figure supplement 1B), and consistently the mRNA levels of the kΔ::ade6+ increased dramatically in these cells (Figure 5C and Figure 5—figure supplement 1C). ------- COMMENT: 8eba150e69a624ea 4 pRiqIwjFkbJ9y35iIPlQrSYYg+w Figure 6B; (comment: CHECK endogenous atf1+ was deleted) ------- COMMENT: 8eba150e69a624ea 9 xCbKZnANhFixEzZd8f2L384Cqpk Figure 8A ------- COMMENT: 8eba150e69a624ea 11 pRiqIwjFkbJ9y35iIPlQrSYYg+w Figure 6B; (comment: CHECK endogenous atf1+ was deleted) ------- COMMENT: 8eba150e69a624ea 12 pRiqIwjFkbJ9y35iIPlQrSYYg+w Figure 6B; (comment: CHECK endogenous atf1+ was deleted) ------- COMMENT: 8eba150e69a624ea 13 pRiqIwjFkbJ9y35iIPlQrSYYg+w Figure 6B; (comment: CHECK endogenous atf1+ was deleted) ------- COMMENT: 8eba150e69a624ea 14 hc+StrlvlUY2LAdVGdWF45Z5TfI Figure 6B; (comment: CHECK endogenous atf1 was deleted) ------- COMMENT: 8eba150e69a624ea 15 4rvOas8+7W342QjYbtT/Ua1IGuc Figure 6B, Figure 3-S1; (comment: CHECK endogenous atf1+ was deleted) ------- COMMENT: 8eba150e69a624ea 16 Zwx5I/slN1xwCBsYnzozp8j7TEA Figure 3-S2A B More strikingly, Patf1-atf1(10D/E) also visibly compromised epigenetic silencing even at 30°C (Figure 3—figure supplement 2A, B). ------- COMMENT: 8eba150e69a624ea 17 TeBVbdg7t6HJpGzQFGldnZIUAXI cells at 37°C restored gene silencing rapidly at normal temperature 30°C after being re-plated on medium containing limited adenine (Figure 2A and B). However, when this re-plating assay was applied to dcr1Δ, one of the RNAi mutants, a considerable proportion of cells still emerged as variegated colonies (designated as dcr1ΔV), which was in sharp contrast to wild type cells (Figure 2B). ------- COMMENT: 8eba150e69a624ea 18 dzvFgAeJsWb9h+4dbOAWUmlo7XU Binding affinity between Atf1 and Swi6HP1 is maintained for non- phosphorylatable Atf1(10A/I) at 37°C, but disrupted for phosphomimetic Atf1(10D/E) even at 30°C. ------- COMMENT: 8eba150e69a624ea 19 dzvFgAeJsWb9h+4dbOAWUmlo7XU Binding affinity between Atf1 and Swi6HP1 is maintained for non- phosphorylatable Atf1(10A/I) at 37°C, but disrupted for phosphomimetic Atf1(10D/E) even at 30°C. ------- COMMENT: 8eba150e69a624ea 20 dj2Pumu6gBljw9eNNs8PppEroYQ Furthermore, in vitro pull-down assays demonstrated that the interaction between Atf1 and Swi6HP1 was also largely disrupted in wis1-DD mutants (Figure 5E and Figure 5—figure supplement 1E). ------- COMMENT: 8eba150e69a624ea 21 KOV4WsHihzq3VjFcHBxReJnV/eY Consistently, ChIP- qPCR analyses showed that the abundance of both H3K9me3 and Swi6HP1 bound at the mat locus but not at pericentromere decreased dramatically in cells with two copies of wis1-DD (Figure 5F and Figure 5—figure supplement 1F). ------- COMMENT: 8eba150e69a624ea 22 KOV4WsHihzq3VjFcHBxReJnV/eY Consistently, ChIP- qPCR analyses showed that the abundance of both H3K9me3 and Swi6HP1 bound at the mat locus but not at pericentromere decreased dramatically in cells with two copies of wis1-DD (Figure 5F and Figure 5—figure supplement 1F). ------- COMMENT: 8eba150e69a624ea 23 LHnAB124eciNuBO3igKuBsGLkmw Intriguingly, removal of Sty1 kinase activity by introducing either sty1 deletion mutant (sty1Δ) or ATP analogue-sensitive mutant of sty1 (sty1-T97A, i.e. sty1-as2) (Zuin et al., 2010) into wis1-DD mutant background could relieve the negative effect of constitutive activation of MAPK Sty1 on kΔ::ade6+ reporter gene silencing, binding affinity between Atf1 and Swi6HP1 and heterochromatin stability at the mat locus (Figure 5 and Figure 5—figure supplement 2). ------- COMMENT: 8eba150e69a624ea 24 LHnAB124eciNuBO3igKuBsGLkmw Intriguingly, removal of Sty1 kinase activity by introducing either sty1 deletion mutant (sty1Δ) or ATP analogue-sensitive mutant of sty1 (sty1-T97A, i.e. sty1-as2) (Zuin et al., 2010) into wis1-DD mutant background could relieve the negative effect of constitutive activation of MAPK Sty1 on kΔ::ade6+ reporter gene silencing, binding affinity between Atf1 and Swi6HP1 and heterochromatin stability at the mat locus (Figure 5 and Figure 5—figure supplement 2). ------- COMMENT: 8eba150e69a624ea 25 LHnAB124eciNuBO3igKuBsGLkmw Intriguingly, removal of Sty1 kinase activity by introducing either sty1 deletion mutant (sty1Δ) or ATP analogue-sensitive mutant of sty1 (sty1-T97A, i.e. sty1-as2) (Zuin et al., 2010) into wis1-DD mutant background could relieve the negative effect of constitutive activation of MAPK Sty1 on kΔ::ade6+ reporter gene silencing, binding affinity between Atf1 and Swi6HP1 and heterochromatin stability at the mat locus (Figure 5 and Figure 5—figure supplement 2). ------- COMMENT: 8eba150e69a624ea 26 LHnAB124eciNuBO3igKuBsGLkmw Intriguingly, removal of Sty1 kinase activity by introducing either sty1 deletion mutant (sty1Δ) or ATP analogue-sensitive mutant of sty1 (sty1-T97A, i.e. sty1-as2) (Zuin et al., 2010) into wis1-DD mutant background could relieve the negative effect of constitutive activation of MAPK Sty1 on kΔ::ade6+ reporter gene silencing, binding affinity between Atf1 and Swi6HP1 and heterochromatin stability at the mat locus (Figure 5 and Figure 5—figure supplement 2). ------- COMMENT: 8eba150e69a624ea 38 X2UdK22eg1pkCnBpQp6Z5GHvIhg indicating full rescue of silencing defects at the mat locus under heat stress. ------- COMMENT: 8eba150e69a624ea 39 iEWI/TJ2us9qIeCrvy6SsV1GhI8 demonstrating that tethering Swi6HP1 to the mat locus was sufficient to rescue heat stress-induced defective epigenetic mainte- nance of heterochromatin. ------- COMMENT: 8ebbc7dc165ca3b5 9 kyqLO89Zuet2iX2DTK0/yCHyNCw the ring seems to start off forming normally but maturation is delayed, this leads to delayed constriction. ------- COMMENT: 8ebbc7dc165ca3b5 17 tkSvHSizcmp4RehtH92JFYmNIxE (comment: independent of actin) ------- COMMENT: 8ebbc7dc165ca3b5 54 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: 8ebbc7dc165ca3b5 55 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: 8eca88feb01d8e15 1 MkxS0vW6XuW2WNurv+H156H2GW4 This result corroborated our crystallographic finding and confirmed that Poz1 indeed exists as a dimer in solution. ------- COMMENT: 8eca88feb01d8e15 3 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 8eca88feb01d8e15 4 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 8eca88feb01d8e15 5 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 8eca88feb01d8e15 6 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 8eca88feb01d8e15 7 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 8eca88feb01d8e15 9 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: 8eca88feb01d8e15 10 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: 8eca88feb01d8e15 11 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: 8eca88feb01d8e15 12 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: 8eca88feb01d8e15 13 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 8eca88feb01d8e15 14 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 8eca88feb01d8e15 15 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 8eca88feb01d8e15 16 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 8eca88feb01d8e15 17 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: 8eca88feb01d8e15 21 9Xb+uz4ftlqagKGp7TY1gJc7WdA Fig. 4F ------- COMMENT: 8eca88feb01d8e15 22 9Xb+uz4ftlqagKGp7TY1gJc7WdA Fig. 4F ------- COMMENT: 8eca88feb01d8e15 23 9Xb+uz4ftlqagKGp7TY1gJc7WdA Fig. 4F ------- COMMENT: 8eca88feb01d8e15 24 9Xb+uz4ftlqagKGp7TY1gJc7WdA Fig. 4F ------- COMMENT: 8eca88feb01d8e15 25 9Xb+uz4ftlqagKGp7TY1gJc7WdA Fig. 4F ------- COMMENT: 8eca88feb01d8e15 26 9Xb+uz4ftlqagKGp7TY1gJc7WdA Fig. 4F ------- COMMENT: 8eca88feb01d8e15 27 9Xb+uz4ftlqagKGp7TY1gJc7WdA Fig. 4F ------- COMMENT: 8eca88feb01d8e15 28 9Xb+uz4ftlqagKGp7TY1gJc7WdA Fig. 4F ------- COMMENT: 8eca88feb01d8e15 29 9Xb+uz4ftlqagKGp7TY1gJc7WdA Fig. 4F ------- COMMENT: 8eca88feb01d8e15 30 9Xb+uz4ftlqagKGp7TY1gJc7WdA Fig. 4F ------- COMMENT: 8eca88feb01d8e15 31 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 8eca88feb01d8e15 32 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 8eca88feb01d8e15 33 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 8eca88feb01d8e15 34 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 8eca88feb01d8e15 35 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 8eca88feb01d8e15 36 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 8eca88feb01d8e15 37 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 8eca88feb01d8e15 38 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 8eca88feb01d8e15 39 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 8eca88feb01d8e15 40 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 8eca88feb01d8e15 41 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 8eca88feb01d8e15 42 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 8eca88feb01d8e15 43 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 8eca88feb01d8e15 44 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 8eca88feb01d8e15 45 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 8eca88feb01d8e15 46 mp0M7zDIFggn53e2MCluP++FNgs Fig. 5E ------- COMMENT: 8eca88feb01d8e15 47 mp0M7zDIFggn53e2MCluP++FNgs Fig. 5E ------- COMMENT: 8eca88feb01d8e15 48 mp0M7zDIFggn53e2MCluP++FNgs Fig. 5E ------- COMMENT: 8eca88feb01d8e15 49 mp0M7zDIFggn53e2MCluP++FNgs Fig. 5E ------- COMMENT: 8eca88feb01d8e15 50 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: 8eca88feb01d8e15 51 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: 8eca88feb01d8e15 52 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: 8eca88feb01d8e15 53 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: 8eca88feb01d8e15 54 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: 8f43f684255db562 2 vTPDJGojerOKVlXnKRECdXuREvI Additionally, the non-essential uncharacterized protein encoded by SPCC594.01 emerged as a top hit in purifications from both its3-TurboID and opy1-TurboID cells (Fig. 1A; Table S1) ------- COMMENT: 8f43f684255db562 3 vTPDJGojerOKVlXnKRECdXuREvI Additionally, the non-essential uncharacterized protein encoded by SPCC594.01 emerged as a top hit in purifications from both its3-TurboID and opy1-TurboID cells (Fig. 1A; Table S1) ------- COMMENT: 8f43f684255db562 4 Ig6SRn4ECAUCMrXH9J6hu/gwFjE To determine if Scs2 and Duc1 directly interact we recombinantly produced His6-Scs2(1-127), which encodes the MSP domain, and GST-Duc1(351-380), which contain the FFAT-like sequence. In vitro binding assays confirmed that the Scs2 MSP domain directly bound Duc1, but not GST alone (Figure 4E). ------- COMMENT: 8f43f684255db562 7 X52kVgWXcYGnJWy1JziXetMtdvI check was plasma membrane /To dissect the contribution of each class of ER-PM contact to Duc1-mNG localization, we analyzed its distribution in hob2Δ, ltc2Δ, ist2Δ, and tcb1Δ tcb2Δ tcb3Δ strains. We found that Duc1-mNG was excluded from the cell division site in all four strains, as in wildtype cells (Fig. S2A). ------- COMMENT: 8f43f684255db562 8 jaen9AiJNJ/cT5HopE1qq8BDovI To dissect the contribution of each class of ER-PM contact to Duc1-mNG localization, we analyzed its distribution in hob2Δ, ltc2Δ, ist2Δ, and tcb1Δ tcb2Δ tcb3Δ strains. We found that Duc1-mNG was excluded from the cell division site in all four strains, as in wildtype cells (Fig. S2A). ------- COMMENT: 8f43f684255db562 9 HhT52CGsAnDJLiLt/Dsxu2tiIhk The localizations of Scs2-mNG, Scs22-mNG and mCherry-AHDL all appeared unchanged in duc1Δ cells compared to wildtype cells (Figure S4B). ------- COMMENT: 8f43f684255db562 10 jaen9AiJNJ/cT5HopE1qq8BDovI To dissect the contribution of each class of ER-PM contact to Duc1-mNG localization, we analyzed its distribution in hob2Δ, ltc2Δ, ist2Δ, and tcb1Δ tcb2Δ tcb3Δ strains. We found that Duc1-mNG was excluded from the cell division site in all four strains, as in wildtype cells (Fig. S2A). ------- COMMENT: 8f43f684255db562 11 MKIHNk5pIHAjvejizV0e2G4a+bI In contrast to its exclusion from the division site in wildtype cells, Duc1- mNG localized along the septa in scs2Δ scs22Δ cells (Fig. 2A,B). This observation was confirmed by live-cell time-lapse imaging with CR and SPB markers (Fig. 2C). Thus, we conclude that Duc1 exclusion from the cell division site depends specifically on Scs2 and Scs22 and not ER-PM contact sites per se. ------- COMMENT: 8f43f684255db562 12 F0ffIkF+JQk2QovBbTGQiHpl1n4 Analysis of Duc1-mNG localization showed that, unlike in wildtype cells, Duc1- mNG localized at the septum in scs2-T39A,T40A scs22Δ cells (Fig. 4F). ------- COMMENT: 8f43f684255db562 13 gc1BTCGeOgCWpJRTTsGxSnSy8fg (comment: CHECK (mislocalized to division site).) As anticipated if these mutations successfully disrupted Duc1 association with Scs2 and Scs22, Duc1-Y374A,F375A-mNG localized at the division site (Fig. 4H). ------- COMMENT: 8f43f684255db562 14 GDmxCr41f9CiFlg6h1Y8brBq6tA Also, Opy1 and Its3 displayed localization along the cortex consistently throughout the cell cycle whereas Duc1 accumulated at different cortical sites depending on the cell-cycle stage (Fig. 1E). These results indicate that Duc1 may be proximal to Its3 and Opy1 at the PM only at certain times. || In scs2Δ scs22Δ cells, Duc1-mNG remained along the cell cortex, confirming its PM localization (Fig. 2A). ------- COMMENT: 8f43f684255db562 16 Dv2z33zevBRtgnQiFGI0/2DwjPU Further, we noticed that over-production of Duc1, like overproduction of Opy1 (Snider et al., 2020), resulted in cells with off- centered septation (Fig. 5E,F), consistent with both of these proteins associating with PI(4,5)P2 and out-competing CR anchoring proteins for a limiting amount of PI(4,5)P2. ------- COMMENT: 8f43f684255db562 17 Zt9ewe4AfUIKQlFCdVnlk2ZJqHk Live-cell imaging revealed that over-production of Duc1 indeed reduced Opy1-mNG at the PM by ~35% (Fig. 5C,D). ------- COMMENT: 8f43f684255db562 18 Zt9ewe4AfUIKQlFCdVnlk2ZJqHk Live-cell imaging revealed that over-production of Duc1 indeed reduced Opy1-mNG at the PM by ~35% (Fig. 5C,D). ------- COMMENT: 8f43f684255db562 19 qlaE1WrCFnITywS46W8sjQWYC24 Live-cell imaging revealed that they all localized either uniformly or in a punctate pattern along the lateral cell cortex and, all but Ist2 lacked any detectable localization at the cell division site (Fig. 3A) ------- COMMENT: 8f43f684255db562 20 qlaE1WrCFnITywS46W8sjQWYC24 Live-cell imaging revealed that they all localized either uniformly or in a punctate pattern along the lateral cell cortex and, all but Ist2 lacked any detectable localization at the cell division site (Fig. 3A) ------- COMMENT: 8f43f684255db562 21 qlaE1WrCFnITywS46W8sjQWYC24 Live-cell imaging revealed that they all localized either uniformly or in a punctate pattern along the lateral cell cortex and, all but Ist2 lacked any detectable localization at the cell division site (Fig. 3A) ------- COMMENT: 8f43f684255db562 22 qlaE1WrCFnITywS46W8sjQWYC24 Live-cell imaging revealed that they all localized either uniformly or in a punctate pattern along the lateral cell cortex and, all but Ist2 lacked any detectable localization at the cell division site (Fig. 3A) ------- COMMENT: 8f43f684255db562 23 LRSoh/mOwT1TjsmrYE3T8BL1Hzw Duc1-mNG levels were also reduced at the lateral PM in its3-1 cells at a semi-restrictive temperature compared to wildtype (Fig. 5A,B). These results are consistent with Duc1 requiring PM PI(4,5)P2 for its PM localization. ------- COMMENT: 8f43f684255db562 24 mVoE+W6bCq/GIflLncEQ+n9wm5s (comment: CHECK (check was normal protein localization to plasma membrane)) We found that Duc1-mNG PM localization along the lateral cell cortex was diminished by ~25% in efr3Δ cells compared to wildtype (Fig. 5A,B). ------- COMMENT: 8f43f684255db562 26 Lc1NrTmawKYd2PVYGTtowKH4PSY There was no significant change in lateral cortex or septum PI4P levels, as determined by the GFP-P4CSidC biosensor (Fig. 6D-F). ------- COMMENT: 8f43f684255db562 27 pKvkcX+nUnHY+TqtUu1W4GqSC2c In contrast, lateral PM and septum PI(4,5)P2 levels, indicated by the GFP-2xPHPlc biosensor, were 36% and 48%, respectively, in duc1Δ compared to wildtype (Fig. 6D-F). ------- COMMENT: 8f43f684255db562 28 SNDhewCARNZN1rLlPLnqmiPU5yU We detected no change in lateral cortex Efr3-mNG but a ~30% decrease in lateral cortex Its3-mNG in duc1Δ compared to wildtype cells (Fig 6G,H and Fig. S4C,D) ------- COMMENT: 8f43f684255db562 29 Q7hiMGzDNz9lVcdxqxLC4ws1HIk Its3-mNG was increased at the septum in scs2Δ scs22Δ cells compared to wildtype cells by ~50% without a change in the lateral cortex levels (Fig. 7A-C). ------- COMMENT: 8f43f684255db562 30 zyc2chPsu8J8eouCOYIhqNYKn9c We confirmed a lack of change in lateral cortical PI(4,5)P2 signal but found that the PI(4,5)P2 signal was increased at the septum by ~20% in scs2Δ scs22Δ cells compared to wildtype (Fig. 7D-F). ------- COMMENT: 8f43f684255db562 31 o83t49RLO1m1OILDGzYoDh46Fxw However, a quantification of the ratio of short- to-long duc1Δ cells at septation demonstrated a significant fraction of duc1Δ cells divided asymmetrically (Fig. 6A,B). Wildtype cells formed a medial CR that slid in 1/13 cells examined, but duc1Δ cells formed a medial CR that slid along the cortex towards one cell end in 12/15 cells observed (Fig. 6C). Thus, Duc1 promotes proper CR anchoring. ------- COMMENT: 8f43f684255db562 32 7YAwjyqu0/AAmjwENk4UGb3UlqE We also examined if Scs2 or Scs22 alone was responsible for preventing Duc1 division site localization but, as in wildtype cells, Duc1- mNG was excluded from the cell division site in both single deletion strains (Fig. S2B). ------- COMMENT: 8f43f684255db562 33 7YAwjyqu0/AAmjwENk4UGb3UlqE We also examined if Scs2 or Scs22 alone was responsible for preventing Duc1 division site localization but, as in wildtype cells, Duc1- mNG was excluded from the cell division site in both single deletion strains (Fig. S2B). ------- COMMENT: 8f43f684255db562 34 HhT52CGsAnDJLiLt/Dsxu2tiIhk The localizations of Scs2-mNG, Scs22-mNG and mCherry-AHDL all appeared unchanged in duc1Δ cells compared to wildtype cells (Figure S4B). ------- COMMENT: 8f43f684255db562 35 SNDhewCARNZN1rLlPLnqmiPU5yU We detected no change in lateral cortex Efr3-mNG but a ~30% decrease in lateral cortex Its3-mNG in duc1Δ compared to wildtype cells (Fig 6G,H and Fig. S4C,D) ------- COMMENT: 8f43f684255db562 47 y0k7+Ta+uPVadBy71bOXACJAoXo (comment: CHECK check was plasma menbrane/) To dissect the contribution of each class of ER-PM contact to Duc1-mNG localization, we analyzed its distribution in hob2Δ, ltc2Δ, ist2Δ, and tcb1Δ tcb2Δ tcb3Δ strains. We found that Duc1-mNG was excluded from the cell division site in all four strains, as in wildtype cells (Fig. S2A). ------- COMMENT: 8f43f684255db562 49 E8PptZQnFEdaVyg1hQHfAEqjrFY There was no change in Its3-mNG septum intensity in duc1Δ cells compared to wildtype, as expected (Fig. S4E). ------- COMMENT: 8f43f684255db562 60 qlaE1WrCFnITywS46W8sjQWYC24 Live-cell imaging revealed that they all localized either uniformly or in a punctate pattern along the lateral cell cortex and, all but Ist2 lacked any detectable localization at the cell division site (Fig. 3A) ------- COMMENT: 8f43f684255db562 61 qlaE1WrCFnITywS46W8sjQWYC24 Live-cell imaging revealed that they all localized either uniformly or in a punctate pattern along the lateral cell cortex and, all but Ist2 lacked any detectable localization at the cell division site (Fig. 3A) ------- COMMENT: 8f43f684255db562 62 qlaE1WrCFnITywS46W8sjQWYC24 Live-cell imaging revealed that they all localized either uniformly or in a punctate pattern along the lateral cell cortex and, all but Ist2 lacked any detectable localization at the cell division site (Fig. 3A) ------- COMMENT: 8f43f684255db562 63 qlaE1WrCFnITywS46W8sjQWYC24 Live-cell imaging revealed that they all localized either uniformly or in a punctate pattern along the lateral cell cortex and, all but Ist2 lacked any detectable localization at the cell division site (Fig. 3A) ------- COMMENT: 8f43f684255db562 64 qlaE1WrCFnITywS46W8sjQWYC24 Live-cell imaging revealed that they all localized either uniformly or in a punctate pattern along the lateral cell cortex and, all but Ist2 lacked any detectable localization at the cell division site (Fig. 3A) ------- COMMENT: 8f47d042c3550bb8 1 TObTO9f3mGUwcnY8pWq+zK8Cd/4 (comment: assayed using assembled Arp2/3 complex, so perhaps some subunits should have contributes_to (but most subunits, maybe all, make contact with actin in the model in http://jcb.rupress.org/content/180/5/887)) ------- COMMENT: 8f47d042c3550bb8 2 TObTO9f3mGUwcnY8pWq+zK8Cd/4 (comment: assayed using assembled Arp2/3 complex, so perhaps some subunits should have contributes_to (but most subunits, maybe all, make contact with actin in the model in http://jcb.rupress.org/content/180/5/887)) ------- COMMENT: 8f47d042c3550bb8 3 TObTO9f3mGUwcnY8pWq+zK8Cd/4 (comment: assayed using assembled Arp2/3 complex, so perhaps some subunits should have contributes_to (but most subunits, maybe all, make contact with actin in the model in http://jcb.rupress.org/content/180/5/887)) ------- COMMENT: 8f47d042c3550bb8 4 TObTO9f3mGUwcnY8pWq+zK8Cd/4 (comment: assayed using assembled Arp2/3 complex, so perhaps some subunits should have contributes_to (but most subunits, maybe all, make contact with actin in the model in http://jcb.rupress.org/content/180/5/887)) ------- COMMENT: 8f47d042c3550bb8 5 TObTO9f3mGUwcnY8pWq+zK8Cd/4 (comment: assayed using assembled Arp2/3 complex, so perhaps some subunits should have contributes_to (but most subunits, maybe all, make contact with actin in the model in http://jcb.rupress.org/content/180/5/887)) ------- COMMENT: 8f47d042c3550bb8 6 TObTO9f3mGUwcnY8pWq+zK8Cd/4 (comment: assayed using assembled Arp2/3 complex, so perhaps some subunits should have contributes_to (but most subunits, maybe all, make contact with actin in the model in http://jcb.rupress.org/content/180/5/887)) ------- COMMENT: 8f47d042c3550bb8 7 TObTO9f3mGUwcnY8pWq+zK8Cd/4 (comment: assayed using assembled Arp2/3 complex, so perhaps some subunits should have contributes_to (but most subunits, maybe all, make contact with actin in the model in http://jcb.rupress.org/content/180/5/887)) ------- COMMENT: 8f53c0c80571a308 5 rRs3aeUME4NBua/V7poQDnl84eY (comment: (it basically the same as cut except the nucleus is not bisected)) ------- COMMENT: 8f53c0c80571a308 13 rRs3aeUME4NBua/V7poQDnl84eY (comment: (it basically the same as cut except the nucleus is not bisected)) ------- COMMENT: 8f6a751681cb343e 7 CEUIZg7aan/BFs55Ma2KODV5nyg (comment: CONDITION 28 celcius) ------- COMMENT: 8f6a751681cb343e 9 nt/Y7zSDPWkShYnsbZexxwtmr8I (Fig. 1C ------- COMMENT: 8f6a751681cb343e 10 nt/Y7zSDPWkShYnsbZexxwtmr8I (Fig. 1C ------- COMMENT: 8f6a751681cb343e 11 lh6cfwYrVg1A0YG2XgC27tfDuMo (Fig. 1C (comment: CONDITION 28 Celcius) ------- COMMENT: 8f6a751681cb343e 12 nt/Y7zSDPWkShYnsbZexxwtmr8I (Fig. 1C ------- COMMENT: 8f6a751681cb343e 13 nt/Y7zSDPWkShYnsbZexxwtmr8I (Fig. 1C ------- COMMENT: 8f6a751681cb343e 14 nt/Y7zSDPWkShYnsbZexxwtmr8I (Fig. 1C ------- COMMENT: 8f6a751681cb343e 22 RUmFhnNdpLlarO7qpu64pK50d/8 ~30% of the double mutant cells exhibited the monopolar spindle phenotype. ------- COMMENT: 8f6a751681cb343e 28 S8zQvXyQzxo8h2j9/hwZoA1Y5tk fig 4. Klp2 is not required, but acts collaboratively with Pkl1, in anchoring the spindle microtubule to the mitotic SPB ------- COMMENT: 8f6a751681cb343e 31 L52EYo+nVzldig3nO8VbQU8Hjjc (comment: often in a punctate manner) ------- COMMENT: 8f9a11dd49d50293 1 cXtdgXbRnJiZ51MmR/4BDbxCwsI These results imply that: (1) Pho84 is multiply ubiquitinated; (2) ubiquitination of Pho84 depends mainly on Pqr1. ------- COMMENT: 8f9a11dd49d50293 5 ZZUEJYA58+VOOt0gPonwIe2rg+0 (comment: CHECK rescued by double deletion of pho84 and pho842) ------- COMMENT: 8f9a11dd49d50293 6 SWDDUSBGdid2YREUMFuNUyA+aOw (comment: CHECK rescued by double deletion of pho84 and pho843) ------- COMMENT: 8f9a11dd49d50293 63 4LHtvG+bx+L4oiyDEzNv324e3T4 inferred from polyphosphate absent from cell ------- COMMENT: 8fac2b6e6e2e1578 1 DNbyXieCEbk1ommni7hPHFArlNw Figure 1D, 2D (comment: - examined via RT-PCR) Figure 2A (comment: examined via RNA-Seq) ------- COMMENT: 8fac2b6e6e2e1578 2 8Hy3BqFFXLt6BygpBw3HupWweMk Figure 1C (comment: transient) ------- COMMENT: 8fac2b6e6e2e1578 3 ifahMAO75xCPaWD3Y3KTLoCbS8E Figure 1D (comment: efficiency/ of introns displaying weak splice sites) ------- COMMENT: 8fac2b6e6e2e1578 4 ifahMAO75xCPaWD3Y3KTLoCbS8E Figure 1D (comment: efficiency/ of introns displaying weak splice sites) ------- COMMENT: 8fac2b6e6e2e1578 5 3SX/ZIkkECk8Hor4/TKu1K3YAL4 Figure 1A-C (comment: transient growth arrest) ------- COMMENT: 8fac2b6e6e2e1578 6 KknSPiafWtvXdClqx1qo+F14KUg Figure 3, 5 (comment: efficiency/ of introns displaying weak splice sites) ------- COMMENT: 8fac2b6e6e2e1578 7 q2b3dzEsY6HUbnVpjMYmsDirm3I Figure 4 (comment: efficiency of introns displaying weak splice sites) ------- COMMENT: 8fac2b6e6e2e1578 8 iAcbpl1ew+nFwb8AiAqOLZxpvN8 Figure 1D ------- COMMENT: 8fac2b6e6e2e1578 9 iAcbpl1ew+nFwb8AiAqOLZxpvN8 Figure 1D ------- COMMENT: 8fac2b6e6e2e1578 10 uOyYkqIJOXU+0+oxbAqORQvq7vA (Fig. S1) ------- COMMENT: 8fac2b6e6e2e1578 11 hqQOPc3Aq7m9CP4atgywTMLv1hI (comment: regulation of efficiency at weak donor) ------- COMMENT: 8fac2b6e6e2e1578 14 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 8fac2b6e6e2e1578 16 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 8fac2b6e6e2e1578 17 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 8fac2b6e6e2e1578 18 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 8fac2b6e6e2e1578 19 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 8fac2b6e6e2e1578 20 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 8fac2b6e6e2e1578 21 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 8fac2b6e6e2e1578 22 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 8fac2b6e6e2e1578 23 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 8fac2b6e6e2e1578 24 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 8fac2b6e6e2e1578 25 Ag6GoP3zsrPsg4IZ5Mmwasgbws4 Fig3 ------- COMMENT: 8fac2b6e6e2e1578 26 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 8fac2b6e6e2e1578 27 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 8fac2b6e6e2e1578 28 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 8fac2b6e6e2e1578 29 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 8fac2b6e6e2e1578 30 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 8fac2b6e6e2e1578 31 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 8fac2b6e6e2e1578 32 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 8fac2b6e6e2e1578 33 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 8fac2b6e6e2e1578 34 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: 8fac2b6e6e2e1578 35 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: 8fac2b6e6e2e1578 36 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: 8fac2b6e6e2e1578 37 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: 8fac2b6e6e2e1578 38 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: 8fac2b6e6e2e1578 39 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: 8fac2b6e6e2e1578 40 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: 8fac2b6e6e2e1578 41 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: 8fb498d6aad39666 1 WcZLaIBZkrZH7qsBmqIeL6+Xi0c Fig 1 (comment: tip1 expressed from pREP3X) ------- COMMENT: 8fb498d6aad39666 2 WcZLaIBZkrZH7qsBmqIeL6+Xi0c Fig 1 (comment: tip1 expressed from pREP3X) ------- COMMENT: 8fb498d6aad39666 3 WcZLaIBZkrZH7qsBmqIeL6+Xi0c Fig 1 (comment: tip1 expressed from pREP3X) ------- COMMENT: 8fb498d6aad39666 4 XZVR4rrs64mxC1Cl1ydVJug21uM Fig 1 (comment: tip1 expressed from pREP3X. I've used this term as it is the nearest to schmoozing which is the term they use in the paper. To be honest I think tapered is better as they don't know that the cells are shmooing) ------- COMMENT: 8fb498d6aad39666 5 Vg36Pb3XgZZRHTX+vSWCnG6rMUM Fig 3B ------- COMMENT: 8fb498d6aad39666 6 L2os8ptCtgreBuhqXtyqppPjqj8 Fig 3H ((comment: methanol fixation)) ------- COMMENT: 8fb498d6aad39666 7 iugwOICngLmkyma0auuN7ZPWnmQ Fig 3D ------- COMMENT: 8fb498d6aad39666 8 VgC27gEeBah9FUdIh3Zfw8FzDbU Fig 3F (comment: (methanol fixation)) ------- COMMENT: 8fb498d6aad39666 9 HjZ8zQil07zsvXjUglO9UCjS25U Figures 4C, 4G ------- COMMENT: 8fb498d6aad39666 10 X9ky+fHCJkDPyRMRh1ZJbt+Xe1Y ( data for these cells not shown) ------- COMMENT: 8fb498d6aad39666 12 0gcMSI18MgD3USiRmriu2ud36QE Fig 3K (comment: (methanol fixation)) ------- COMMENT: 8fb498d6aad39666 13 0gcMSI18MgD3USiRmriu2ud36QE Fig 3K (comment: (methanol fixation)) ------- COMMENT: 8fb498d6aad39666 14 nUFaJcA+R2lQsdvrwODz9ku3nnM Fig3I ------- COMMENT: 8fb498d6aad39666 15 2Cv9LNU4lUsZcBCAegdJgsMHvlM Fig 3I (comment: (Formaldehyde fixation)) ------- COMMENT: 8fb498d6aad39666 16 /tu3QbB3owrlj8QrxQ7TooH2L6M Fig 4A, fig 3G ------- COMMENT: 8fb498d6aad39666 17 69cQ0fDndIKtQRowjK3rCl3sw3c Fig 4F However, tip1p was observed at the tips of the astral microtubules that ema- nated from the cytoplasmic face of the nuclear-located spindle pole body during anaphase and at the tips of the microtubules generated from the central region of postmitotic cells (Figures 4F). ------- COMMENT: 8fb498d6aad39666 18 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: 8fb498d6aad39666 20 zV3UF6KHU0cP3xV2/nVfIkVqXJU Fig 6 (comment: (live cell imaging) GFP-tubulin expressed from nmt1 promoter on multi copy plasmid) ------- COMMENT: 8fb498d6aad39666 21 ayzzkiljnX8KLeVfOiGnZf+tnGA Fig 6 (comment: (live cell imaging) GFP-tubulin expressed from nmt1 promoter on multi copy plasmid) ------- COMMENT: 8fb498d6aad39666 22 xVNAOgV9097ydaHPhWH1qDTl9VY Fig 3 (comment: (methanol fixation)) ------- COMMENT: 8fb498d6aad39666 24 qx3WcvjTjwGaFyxtWL1XpJeJChI Fig 3C ------- COMMENT: 8fb498d6aad39666 27 fNu0+vQoFrTl9I7CMAo8UCdc2hs Fig 1B ------- COMMENT: 8fb498d6aad39666 28 93lO9fKo+pfeeqdP+WODl7I5DaY "These phenotypes establish that tip1p is required to properly position the growth zones at the antipodes of the cells." ------- COMMENT: 8fb498d6aad39666 29 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 8fb498d6aad39666 30 uqt3TKIo9eLvn/IHVviBOWWsh70 "We conclude that tip1p is required for the correct organization of the microtubule cytoskeleton and for the proper localization of the tea1p marker to the cell ends" ------- COMMENT: 8fb498d6aad39666 31 ca1k9evFlu6at+AXppQNvII6SQA figure 4H (comment: CHECK in vitro) ------- COMMENT: 8fb498d6aad39666 32 2m26LSsEUhMtzgdVB0B7Uh+fmng (Figure 4I) (comment: I'm not sure if we knew it was the plus end then, but we do now ;)) ------- COMMENT: 8fb498d6aad39666 33 mxWxtFj9vZKP6ayalZwMJhoc80M (Figure 4I) ------- COMMENT: 8fbfc2228b1d145e 1 vGtIAlSSPNnMrmPTCV9WpCBeLHE (Fig. 7B) ------- COMMENT: 8fbfc2228b1d145e 8 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: 8fbfc2228b1d145e 9 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: 8fbfc2228b1d145e 10 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: 8fbfc2228b1d145e 11 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: 8fbfc2228b1d145e 12 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: 8fbfc2228b1d145e 13 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: 8fbfc2228b1d145e 14 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: 8fbfc2228b1d145e 15 scLHnxlZahYRJPv+7UnEhy1UEic (Fig. 2A, 3A) ------- COMMENT: 8fbfc2228b1d145e 16 baShzNPxM26FnZQ+NSBH5QB4gmE (Fig. 2C, 3A) ------- COMMENT: 8fbfc2228b1d145e 17 baShzNPxM26FnZQ+NSBH5QB4gmE (Fig. 2C, 3A) ------- COMMENT: 8fbfc2228b1d145e 18 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: 8fbfc2228b1d145e 19 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: 8fbfc2228b1d145e 20 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 8fbfc2228b1d145e 21 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: 8fbfc2228b1d145e 22 kuRdbpnFrU9sjksgW9GQwEQ1424 (Fig. 5) ------- COMMENT: 8fbfc2228b1d145e 23 hPFNK9pXn9s6uyKJPTkg3DIM2XE (Fig. 6A) ------- COMMENT: 8fbfc2228b1d145e 24 hPFNK9pXn9s6uyKJPTkg3DIM2XE (Fig. 6A) ------- COMMENT: 8fbfc2228b1d145e 25 sUCS79Rs9xFD1wViTRlsgeGdNFs (Fig. 6B) ------- COMMENT: 8fbfc2228b1d145e 26 sUCS79Rs9xFD1wViTRlsgeGdNFs (Fig. 6B) ------- COMMENT: 8fbfc2228b1d145e 27 sUCS79Rs9xFD1wViTRlsgeGdNFs (Fig. 6B) ------- COMMENT: 8fbfc2228b1d145e 28 sUCS79Rs9xFD1wViTRlsgeGdNFs (Fig. 6B) ------- COMMENT: 8fbfc2228b1d145e 29 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: 8fbfc2228b1d145e 30 j6G2s1mlHnX+grtKdaKc3y1QZCw (Fig. 8B) ------- COMMENT: 8fbfc2228b1d145e 31 j6G2s1mlHnX+grtKdaKc3y1QZCw (Fig. 8B) ------- COMMENT: 8fbfc2228b1d145e 32 j6G2s1mlHnX+grtKdaKc3y1QZCw (Fig. 8B) ------- COMMENT: 8fbfc2228b1d145e 33 j6G2s1mlHnX+grtKdaKc3y1QZCw (Fig. 8B) ------- COMMENT: 8fbfc2228b1d145e 34 j6G2s1mlHnX+grtKdaKc3y1QZCw (Fig. 8B) ------- COMMENT: 8fbfc2228b1d145e 35 j6G2s1mlHnX+grtKdaKc3y1QZCw (Fig. 8B) ------- COMMENT: 8fbfc2228b1d145e 36 a/9dWx0StQxoXUIcLBF0HsvDtKY (Fig. 9A) ------- COMMENT: 8fbfc2228b1d145e 37 XGT7f8Gq1YHjODM0qo4Qzy0rI4w (Fig. 9B) ------- COMMENT: 8fbfc2228b1d145e 38 XGT7f8Gq1YHjODM0qo4Qzy0rI4w (Fig. 9B) ------- COMMENT: 8fbfc2228b1d145e 39 a/9dWx0StQxoXUIcLBF0HsvDtKY (Fig. 9A) ------- COMMENT: 8fbfc2228b1d145e 40 XGT7f8Gq1YHjODM0qo4Qzy0rI4w (Fig. 9B) ------- COMMENT: 8fbfc2228b1d145e 41 XGT7f8Gq1YHjODM0qo4Qzy0rI4w (Fig. 9B) ------- COMMENT: 8fbfc2228b1d145e 42 HUn5xqwsoiC9v7eYxndTBjsYBfM (Fig. 10A) ------- COMMENT: 8fbfc2228b1d145e 43 HUn5xqwsoiC9v7eYxndTBjsYBfM (Fig. 10A) ------- COMMENT: 8fbfc2228b1d145e 44 HUn5xqwsoiC9v7eYxndTBjsYBfM (Fig. 10A) ------- COMMENT: 8fbfc2228b1d145e 45 HUn5xqwsoiC9v7eYxndTBjsYBfM (Fig. 10A) ------- COMMENT: 8fbfc2228b1d145e 46 tbjZyk8FtsauhCybrCMQF6f5pbQ (Fig. 10C) ------- COMMENT: 8fbfc2228b1d145e 47 tbjZyk8FtsauhCybrCMQF6f5pbQ (Fig. 10C) ------- COMMENT: 8fbfc2228b1d145e 48 tbjZyk8FtsauhCybrCMQF6f5pbQ (Fig. 10C) ------- COMMENT: 8fbfc2228b1d145e 49 tbjZyk8FtsauhCybrCMQF6f5pbQ (Fig. 10C) ------- COMMENT: 8fbfc2228b1d145e 50 1OKO1ScUtsLgBQN+fozMA+O4/KA (Fig. 10B) ------- COMMENT: 8fbfc2228b1d145e 51 1OKO1ScUtsLgBQN+fozMA+O4/KA (Fig. 10B) ------- COMMENT: 8fbfc2228b1d145e 52 1OKO1ScUtsLgBQN+fozMA+O4/KA (Fig. 10B) ------- COMMENT: 8fe26054c4dd9003 1 joUktSPUGXl1ezDJB/dL25jTXFA figure1 ------- COMMENT: 8fe26054c4dd9003 2 K1+1XnJGz3kIYfxAy0pgn4guZ+s figure1 (comment: CHECK cdr phenotype) ------- COMMENT: 8fe26054c4dd9003 3 joUktSPUGXl1ezDJB/dL25jTXFA figure1 ------- COMMENT: 8fe26054c4dd9003 4 joUktSPUGXl1ezDJB/dL25jTXFA figure1 ------- COMMENT: 8fe26054c4dd9003 5 eKlQdeiq1wjkh3T9WNqFatVfqR0 figure2a ------- COMMENT: 8fe26054c4dd9003 7 eKlQdeiq1wjkh3T9WNqFatVfqR0 figure2a ------- COMMENT: 8fe26054c4dd9003 9 tw2Ox+vPIVbpojY226CIElxYTJY figure 2C, D ------- COMMENT: 8fe26054c4dd9003 11 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 8fe26054c4dd9003 12 rJUxYYrAUknyCetVV3uMOvM8voU figure 4 (comment: CHECK cdr phenotype) ------- COMMENT: 8fe26054c4dd9003 13 rJUxYYrAUknyCetVV3uMOvM8voU figure 4 (comment: CHECK cdr phenotype) ------- COMMENT: 8fe26054c4dd9003 14 rJUxYYrAUknyCetVV3uMOvM8voU figure 4 (comment: CHECK cdr phenotype) ------- COMMENT: 8fe26054c4dd9003 15 rJUxYYrAUknyCetVV3uMOvM8voU figure 4 (comment: CHECK cdr phenotype) ------- COMMENT: 8fe26054c4dd9003 16 rJUxYYrAUknyCetVV3uMOvM8voU figure 4 (comment: CHECK cdr phenotype) ------- COMMENT: 8fe26054c4dd9003 17 rJUxYYrAUknyCetVV3uMOvM8voU figure 4 (comment: CHECK cdr phenotype) ------- COMMENT: 8fe26054c4dd9003 18 rJUxYYrAUknyCetVV3uMOvM8voU figure 4 (comment: CHECK cdr phenotype) ------- COMMENT: 8fe26054c4dd9003 19 rJUxYYrAUknyCetVV3uMOvM8voU figure 4 (comment: CHECK cdr phenotype) ------- COMMENT: 8fe26054c4dd9003 20 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 8fe26054c4dd9003 21 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 8fe26054c4dd9003 22 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 8fe26054c4dd9003 23 lVvMgkFNoEp4aURnzoIpcy7J//0 fig 5A ------- COMMENT: 8fe26054c4dd9003 24 lVvMgkFNoEp4aURnzoIpcy7J//0 fig 5A ------- COMMENT: 8fe26054c4dd9003 25 lVvMgkFNoEp4aURnzoIpcy7J//0 fig 5A ------- COMMENT: 8fe26054c4dd9003 26 MoxVelGL3QQAQeBoUU79+6ix3ac figure 5C, D ------- COMMENT: 8fe26054c4dd9003 27 ZCc5JmdUcHx48wJNZOtVysapXjQ fig 5C, D ------- COMMENT: 8fe26054c4dd9003 28 lxThXDhufcNcXlVZIa8TqAZkI3k figure 6A ------- COMMENT: 8fe26054c4dd9003 33 g6A/A79USGLSU7eLAhJM0Hxj98Y (comment: semi wee) ------- COMMENT: 8fe26054c4dd9003 34 sLtEESm0GlzMXlfRdKWyYZPhylE figure 6b (comment: CHECK during x phase?) ------- COMMENT: 8fe26054c4dd9003 36 L2o5+XkpBSzDXdRS/Ej502eTDFA table 4 ------- COMMENT: 8fe26054c4dd9003 37 K1+1XnJGz3kIYfxAy0pgn4guZ+s figure1 (comment: CHECK cdr phenotype) ------- COMMENT: 901cd8f6d579af4e 1 rciebarHThye/O38NNxO5olvvDU Mdb1 binds to Hta1 phosphorylated on Ser-129. (comment: CHECK PR:000027566 = H2A phosphorylated on S129) ------- COMMENT: 901cd8f6d579af4e 6 +gUKzWH6gUo0O/MP188AeLbJWUQ This localisation requires phosphorylated histone H2A. ------- COMMENT: 903cb37e16a1d237 1 lRPbaEP9vud9mZgNMAMiuGj+nGU 38.5% of wild-type recombination assayed between ura4+-aim2 and his3+-aim (Fig. 4B, Table S5) ------- COMMENT: 903cb37e16a1d237 2 QlpmrkxoA4dQHw5bwntF7jed090 33.7% of wild-type recombination assayed between ade6-3083 and ade6-469 (Fig. 4A, Table S5) ------- COMMENT: 903cb37e16a1d237 3 MweVwMLPwj6BMPEkwIuJQBuRCcM (Fig. 4C, Table S5) ------- COMMENT: 903cb37e16a1d237 4 gdAT5zhIFRu92WqfrsjSvr6Q/Kc (comment: CHECK 83.9% of wild-type spore viability) (Fig. 4D, Table S5) ------- COMMENT: 903cb37e16a1d237 5 TUuITIl6F2OQCO1WDl0dJhF3dgU (Table S2) ------- COMMENT: 903cb37e16a1d237 6 TUuITIl6F2OQCO1WDl0dJhF3dgU (Table S2) ------- COMMENT: 903cb37e16a1d237 7 NzkbDzTONIqRyOOClFPeMk5FyQQ (comment: CHECK 117% of wild-type recombination assayed at various loci) (Fig. 1, Table S2) ------- COMMENT: 903cb37e16a1d237 8 9VsuGZ6GId2UMR/dt1SIMB49ioQ (comment: CHECK 78.4% of wild-type spore viability) (Table S2) ------- COMMENT: 903cb37e16a1d237 9 0HVkUGw7plFqQr04NU4z23cjlxA (comment: CHECK 28.9% of wild-type recombination assayed between ura4+-aim2 and his3+-aim) (Fig. 4B, Table S5) ------- COMMENT: 903cb37e16a1d237 10 kMyfuwjOzpMyLpf/TQxuT6DPepE (comment: CHECK 39.7% of wild-type recombination assayed between ade6-3083 and ade6-469) (Fig. 4A, Table S5) ------- COMMENT: 903cb37e16a1d237 11 u4KJPSx+luWe2WOUFwiAvADBAHk (comment: CHECK 85.9% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim) (Fig. 4C, Table S5) ------- COMMENT: 903cb37e16a1d237 12 STcZANmS1OObvxC2+byrT3pqZm0 (comment: CHECK 61.1% of wild-type spore viability) (Fig. 4D, Table S5) ------- COMMENT: 903cb37e16a1d237 13 /8BqsoXpCy/+ck7JYs+YkrRstTs (comment: CHECK 61.0% of wild-type recombination assayed between ura4+-aim2 and his3+-aim) (Fig. 3B, Table S4) ------- COMMENT: 903cb37e16a1d237 14 8a/hxF18dGebYOF09tE3to8B0YQ (comment: CHECK 42.4% of wild-type recombination assayed between ade6-3083 and ade6-469) (Fig. 3A, Table S4) ------- COMMENT: 903cb37e16a1d237 15 3w8+RGksyF7H48VREfj9mwgcm7M (comment: CHECK 82.9% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim) (Fig. 4C, Table S5) ------- COMMENT: 903cb37e16a1d237 16 FW9ecJFyrV2RfVUFqo2fZJnIMTo (comment: CHECK 95.2% of wild-type spore viability) (Fig. 3D, Table S4) ------- COMMENT: 903cb37e16a1d237 17 H2CT/+IKKWPhn2zoEaR5OEbJ6rs (comment: CHECK 48.0% of wild-type recombination assayed between ura4+-aim2 and his3+-aim) (Fig. 3B, Table S4) ------- COMMENT: 903cb37e16a1d237 18 m6SZpdJr3zmPNmWFcnJ7rHih09o (comment: CHECK 27.7% of wild-type recombination assayed between ade6-3083 and ade6-469) (Fig. 3A, Table S4) ------- COMMENT: 903cb37e16a1d237 19 buIHtUNTej91FBbs8kmXpS893ng (comment: CHECK 82.2% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim) (Fig. 3C, Table S4) ------- COMMENT: 903cb37e16a1d237 20 5StRUYt6isWos9E9pyCiYPmhKCY (comment: CHECK 108.5% of wild-type spore viability) (Fig. 3D, Table S4) ------- COMMENT: 903cb37e16a1d237 21 xIIgittVIFQ1v7Cmp206lLYEgsE (comment: CHECK 49.0% of wild-type recombination assayed between ura4+-aim2 and his3+-aim) (Fig. 3B, Table S4) ------- COMMENT: 903cb37e16a1d237 22 FqHHC4o3nkRLMIH2dD5t0vnIBgU (comment: CHECK 49.2% of wild-type recombination assayed between ade6-3083 and ade6-469) (Fig. 3A, Table S4) ------- COMMENT: 903cb37e16a1d237 23 wVHdXTYg0AZFPtepIBRgNfElTlM (comment: CHECK 90.5% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim) (Fig. 3C, Table S4) ------- COMMENT: 903cb37e16a1d237 24 cptDaJmr677JAh4UWonuxws1+4U (comment: CHECK 133.5% of wild-type spore viability) (Fig. 3D, Table S4) ------- COMMENT: 903cb37e16a1d237 25 /Y+Rb2h3aA87kB9OLlVX4Ld+cZE (comment: CHECK 16.3% of wild-type recombination assayed between ura4+-aim2 and his3+-aim) (Fig. 4B, Table S5) ------- COMMENT: 903cb37e16a1d237 26 rjkgbOcXF57M3aVrXmAhZIwOKig (comment: CHECK 9.7% of wild-type recombination assayed between ade6-3083 and ade6-469) (Fig. 4A, Table S5) ------- COMMENT: 903cb37e16a1d237 27 jUgR2KHiyLxT2dMIKLL/VnNK/aA (comment: CHECK 87.6% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim) (Fig. 4C, Table S5) ------- COMMENT: 903cb37e16a1d237 28 qw+MBOP2vi1cttfpQOOOAKJhKHk (comment: CHECK 77.7% of wild-type spore viability) (Fig. 4D, Table S5) ------- COMMENT: 903cb37e16a1d237 29 dEX5K4/F/X4SB0dM+Fws2d+FvFU (comment: CHECK 31.4% of wild-type recombination assayed between ura4+-aim2 and his3+-aim) (Table S6) ------- COMMENT: 903cb37e16a1d237 30 3D7TVQZmXwu+IwO5uc+SkFht42Q (comment: CHECK 8.0% of wild-type recombination assayed between ade6-3083 and ade6-469) (Fig. 6, Table S6) ------- COMMENT: 903cb37e16a1d237 31 NwXbjzFPo6yJXpQwMJFX7wbrE5E (Fig. 5, Table S6) ------- COMMENT: 903cb37e16a1d237 32 22fpWc0zsj9Pt0KB4G4D2jHzRSE (comment: CHECK 43.6% of wild-type spore viability) (Table S6) ------- COMMENT: 903cb37e16a1d237 34 OsPEUx/XNweMZPiN8eHm9qfvrU4 (comment: CHECK 0.9% of wild-type recombination assayed between ura4+-aim2 and his3+-aim) (Table S3) ------- COMMENT: 903cb37e16a1d237 35 mD7Vy5/CBVuF9EU94OozAeHKz7A (comment: CHECK 12.3% of wild-type recombination assayed between ade6-3083 and ade6-469) (Table S3) ------- COMMENT: 903cb37e16a1d237 36 OzX2PdbSrf7ueQDM5wBHBb93fss (comment: CHECK 1.7% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim) (Fig. 2B, Table S3) ------- COMMENT: 903cb37e16a1d237 37 7RzYTA0jBKaNJecgljc9LHN31GE (comment: CHECK 47% of wild-type spore viability) (Fig. 2A, Table S3), (comment: 18.3-fold higher spore viability than mus81 single mutant) ------- COMMENT: 903cb37e16a1d237 38 t2pGzI6CPzxn3g0ZQKPHfw4euGI (comment: CHECK 19.1% of wild-type recombination assayed between ura4+-aim2 and his3+-aim) (Table S3) ------- COMMENT: 903cb37e16a1d237 39 KZ+l/vJEjegVXLyaKO6K/G7nDnA (comment: CHECK 2.2% of wild-type recombination assayed between ade6-3083 and ade6-469) (Table S3) ------- COMMENT: 903cb37e16a1d237 40 MyVGusDpg0cua//oKzyvdClFWuE (comment: CHECK 10.5% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim) (Fig. 2B, Table S3) ------- COMMENT: 903cb37e16a1d237 41 hYw2v21HphWuCt72vwpH2uHvtLw (comment: CHECK 42.9% of wild-type spore viability, 16.7-fold higher spore viability than mus81 single mutant) (Fig. 2A, Table S3) ------- COMMENT: 903cb37e16a1d237 42 XIBAFtt8FIcLUG4icmra+RgMbn0 (comment: CHECK 26.0% of wild-type recombination assayed between ura4+-aim2 and his3+-aim) (Table S3) ------- COMMENT: 903cb37e16a1d237 43 YYGbJSy7a8P6GAfeWPADqtEMkLw (comment: CHECK 30.1% of wild-type recombination assayed between ade6-3083 and ade6-469) (Table S3) ------- COMMENT: 903cb37e16a1d237 44 fB49Tww0q9XrBsVGE9/DLz4bQlw (comment: CHECK 9.9% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim) (Fig. 2B, Table S3) ------- COMMENT: 903cb37e16a1d237 45 zIYY5nXpwYSoGf0psg3G+HacEfQ (comment: CHECK 21.2% of wild-type spore viability, 8.3-fold higher spore viability than mus81 single mutant) (Fig. 2A, Table S3) ------- COMMENT: 903cb37e16a1d237 46 yaD27FQbKJOd3ZkQYn5PvABe2l8 (comment: CHECK 6.4% of wild-type recombination assayed between ura4+-aim2 and his3+-aim) (Table S3) ------- COMMENT: 903cb37e16a1d237 47 bK+irtsn7OSwQAGbPA2Z1PX3IE4 (comment: CHECK 57.9% of wild-type recombination assayed between ade6-3083 and ade6-469) (Table S3) ------- COMMENT: 903cb37e16a1d237 48 HhhQ4e3wBxYT+PiE63nndhbAP4I (comment: CHECK 7.8% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim) (Fig. 2B, Table S3) ------- COMMENT: 903cb37e16a1d237 49 WiIvc9TlXh+SlGu17jNY2L5nMbk (comment: CHECK 28.9% of wild-type spore viability, 11.3-fold higher spore viability than mus81 single mutant) (Fig. 2A, Table S3) ------- COMMENT: 903cb37e16a1d237 50 5QdEsOvxvObk1I4u/Iyku1raBxs (comment: CHECK 58.1% of wild-type recombination assayed between ura4+-aim2 and his3+-aim) (Table S3) ------- COMMENT: 903cb37e16a1d237 51 TRbyGSkktxQePT5jUhzYPRpa6rA (comment: CHECK 36.3% of wild-type recombination assayed between ade6-3083 and ade6-469) (Table S3) ------- COMMENT: 903cb37e16a1d237 52 mEdUUyb4fYrQMgjO0fbYsnGyJLU (comment: CHECK 6.9% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim) (Fig. 2B, Table S3) ------- COMMENT: 903cb37e16a1d237 53 hyt25rdxMhx+zvo9znW1yVLCOno (comment: CHECK 44.7% of wild-type spore viability, 17.4-fold higher spore viability than mus81 single mutant) (Fig. 2A, Table S3) ------- COMMENT: 903cb37e16a1d237 54 bedkuwrb7iTnQK7gFEk7AygZ5Ek (comment: CHECK 48.0% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, epistatic relationship) (Fig.3B, Table S4) ------- COMMENT: 903cb37e16a1d237 55 /nUddQ6NB5GrwWFdAJFK0hP7QS4 (comment: CHECK 32.3% of wild-type recombination assayed between ade6-3083 and ade6-469, epistatic relationship) (Fig. 3A, Table S4) ------- COMMENT: 903cb37e16a1d237 56 LZSyMMToLvc2HMqZ2Q9BbOzisdo (comment: CHECK 85.8% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim, epistatic relationship) (Fig. 3C, Table S4) ------- COMMENT: 903cb37e16a1d237 57 VFxucbgSu4FyieZBNkJbcCOx/tM (comment: CHECK 117.1% of wild-type spore viability) (Fig. 3D, Table S4) ------- COMMENT: 903cb37e16a1d237 58 lIkEGMieunPNpfpYqnLo8Lor7fM (comment: CHECK 55.5% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, epistatic relationship) (Fig. 3B, Table S4) ------- COMMENT: 903cb37e16a1d237 59 1ab3NwtJA8e/fzLfKP1CrJysGcQ (comment: CHECK 44.4% of wild-type recombination assayed between ade6-3083 and ade6-469, epistatic relationship) (Fig. 3A, Table S4) ------- COMMENT: 903cb37e16a1d237 60 pnNq7ezFxIlvtORMinKQuUNSGDc (comment: CHECK 78.6% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim, epistatic relationship) (Fig. 3C, Table S4) ------- COMMENT: 903cb37e16a1d237 61 ElxrNps41MIujhlq5PcXDGTqgHs (comment: CHECK 104.8% of wild-type spore viability) (Fig. 3D, Table S4) ------- COMMENT: 903cb37e16a1d237 62 Jh8Jwn8DNZpNyvuh1FJj70A/3mw (comment: CHECK 0% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, synergistic relationship) (Fig. 4B, Table S5) ------- COMMENT: 903cb37e16a1d237 63 /SEP+ctD0ibUrHnxO7KjyPGAjS0 (comment: CHECK 0.42% of wild-type recombination assayed between ade6-3083 and ade6-469, synergistic relationship) (Fig. 4A, Table S5) ------- COMMENT: 903cb37e16a1d237 64 6Y8CW0eREE3cMK+7/cIRk9jUykw (comment: CHECK 3.7% of wild-type spore viability, synergistic relationship) (Fig. 4D, Table S5) ------- COMMENT: 903cb37e16a1d237 65 MwqyAISNupEXQzmXZed6yBGswKc (comment: CHECK 4.5% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, synergistic relationship) (Fig. 4B, Table S5) ------- COMMENT: 903cb37e16a1d237 66 Wk05lOpVL3Fb3is4LaEipOLCFaE (comment: CHECK 2.5% of wild-type recombination assayed between ade6-3083 and ade6-469, synergistic relationship) (Fig. 4A, Table S5) ------- COMMENT: 903cb37e16a1d237 67 MCxzFWnYZ0Wp1vE/rF6BYlHaEvw (comment: CHECK 18.5% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim, synergistic relationship) (Fig. 4C, Table S5) ------- COMMENT: 903cb37e16a1d237 68 QBWK00wRopCpKmwnZ0vGJ01n90M (comment: CHECK 13.0% of wild-type spore viability, synergistic relationship) (Fig. 4D, Table S5) ------- COMMENT: 903cb37e16a1d237 69 Xm1R9x23HvUvYqyfvI/fM0Nd3k0 (comment: CHECK 33.5% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, epistatic relationship) (Fig. 4B, Table S5) ------- COMMENT: 903cb37e16a1d237 70 y9uMKz8rD67tfjEWGx9Q76YlIok (comment: CHECK 14.2% of wild-type recombination assayed between ade6-3083 and ade6-469, epistatic relationship) (Fig. 4A, Table S5) ------- COMMENT: 903cb37e16a1d237 71 U15Wf5Efv6dBRmrCzw2l8xxU4Gg (comment: CHECK 84.1% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim, epistatic relationship) (Fig. 4C, Table S5) ------- COMMENT: 903cb37e16a1d237 72 cBYUZ2nTDlZCEllZqARsgoKgero (comment: CHECK 93.8% of wild-type spore viability, epistatic relationship) (Fig. 4D, Table S5) ------- COMMENT: 903cb37e16a1d237 73 6/2KIpn6gPMO2OQFDns3x85AAD4 (comment: CHECK 26.4% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, epistatic relationship) (Fig. 4B, Table S5) ------- COMMENT: 903cb37e16a1d237 74 MUzCa+wIModNlIKzUWaG1igs5/s (comment: CHECK 25.7% of wild-type recombination assayed between ade6-3083 and ade6-469, epistatic relationship) (Fig. 4A, Table S5) ------- COMMENT: 903cb37e16a1d237 75 3yUrz0GHb16FW0ofOk4pPstnbEo (comment: CHECK 91.4% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim, epistatic relationship) (Fig. 4C, Table S5) ------- COMMENT: 903cb37e16a1d237 76 7eGu0+gl8ixmn5hJJvzANWtBh4I (comment: CHECK 76.9% of wild-type spore viability, epistatic relationship) (Fig. 4D, Table S5) ------- COMMENT: 903cb37e16a1d237 77 Jh8Jwn8DNZpNyvuh1FJj70A/3mw (comment: CHECK 0% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, synergistic relationship) (Fig. 4B, Table S5) ------- COMMENT: 903cb37e16a1d237 78 QeNxBv40LZYDTWQq63XzxQC1aug (comment: CHECK 2.1% of wild-type recombination assayed between ade6-3083 and ade6-469, synergistic relationship) (Fig. 4A, Table S5) ------- COMMENT: 903cb37e16a1d237 79 mtgj6yFp3VYSgFfHKVtgsIdIlGs (comment: CHECK 106.7% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim, epistatic relationship) (Fig. 4C, Table S5) ------- COMMENT: 903cb37e16a1d237 80 FwXCBvIJBi1tUwxDT8iMo+B1TTE (comment: CHECK 9.9% of wild-type spore viability, synergistic relationship) (Fig. 4D, Table S5) ------- COMMENT: 903cb37e16a1d237 81 maDAHPH3dAj05cJyCku3q6F6TrA (comment: CHECK 65.3% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, epistatic relationship) (Fig. 4B, Table S5) ------- COMMENT: 903cb37e16a1d237 82 YhWBU+t6CZJPhD8nnQseLZKmTgU (comment: CHECK 21.1% of wild-type recombination assayed between ade6-3083 and ade6-469, epistatic relationship) (Fig. 4A, Table S5) ------- COMMENT: 903cb37e16a1d237 83 Bu42v0ToovPRKxe6dU4C7CoieUs (comment: CHECK 76.7% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim, synergistic relationship; skewed recombinant classes) (Figs. 4C, 7A, Table S5) ------- COMMENT: 903cb37e16a1d237 84 XdMTPFLogoh1OLXP7lX5o9jV51E (comment: CHECK 55.5% of wild-type spore viability, synergistic relationship) (Fig. 4D, Table S5) ------- COMMENT: 903cb37e16a1d237 85 veDLZIqfkQDLgGxUrMVgTpWULw8 (comment: CHECK 54.0% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, epistatic relationship) (Table S5) ------- COMMENT: 903cb37e16a1d237 86 DGaaYQq6ZI16+/WfsFVAsAwSpl0 (comment: CHECK 31.1% of wild-type recombination assayed between ade6-3083 and ade6-469, epistatic relationship) (Table S5) ------- COMMENT: 903cb37e16a1d237 87 e5ekHAjRft4sr8ywyMLQV2rX1cA (comment: CHECK 75.2% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim, synergistic relationship) (Fig. S2, Table S5) ------- COMMENT: 903cb37e16a1d237 88 siV9vjOVT0fqmSb99qzGfMbTKhg (comment: CHECK 50.7% of wild-type spore viability, synergistic relationship) (Table S5) ------- COMMENT: 903cb37e16a1d237 89 SY/YIJMDZEVT8aK7jW7iJikRtPY (comment: CHECK 15.4% of wild-type spore viability, synergistic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 90 uXOLxBg1MP27gYOuMzgMTE9Qiwk (comment: CHECK 76.1% of wild-type recombination assayed between ade6-3083 and ade6-469) (Table S6) ------- COMMENT: 903cb37e16a1d237 91 FN4gU0BpQBJe6oTzWSrf5Ehfv/E (comment: CHECK 7.2% of wild-type recombination assayed between ade6-3083 and ade6-469, epistatic relationship - similar to rqh1delta) (Fig. 6, Table S6) ------- COMMENT: 903cb37e16a1d237 92 w+TeMC6j2Uhg2NSwUeBREYpjLdQ (comment: CHECK 124.3% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim, epistatic relationship - similar to fml1delta) (Fig. 5, Table S6) ------- COMMENT: 903cb37e16a1d237 93 T1mJQQv/gQqNg0yniGpC2la6af4 (comment: CHECK 60.64% of wild-type spore viability, epistatic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 94 yBTgmO5734jmm0Y8BENFTRSCdwk (comment: CHECK 7.4% of wild-type recombination assayed between ade6-3083 and ade6-469, epistatic relationship) (Fig.6A, Table S6) ------- COMMENT: 903cb37e16a1d237 95 cCX+Afupp9UgmotzirArfDSexfs (comment: CHECK 24.3% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, epistatic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 96 fjkkaXHMscRjNuM+8Op614XX5Ko (comment: CHECK 115.17% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim, epistatic relationship) (Fig. 5A, Table S6) ------- COMMENT: 903cb37e16a1d237 97 p6U3wrJdfHKhRuOl/KT3FwK3V+0 (comment: CHECK 25.9% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, epistatic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 98 suO1L1DMsQB24nSZ9HrHDol8Z0A (comment: CHECK 2.55% of wild-type recombination assayed between ade6-3083 and ade6-469, synergistic relationship) (Fig.6A, Table S6) ------- COMMENT: 903cb37e16a1d237 99 PPfG4zodHD+6ke/Uj1HyoSTk9EA (comment: CHECK 115.12% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim, synergistic relationship) (Fig. 5A, Table S6) ------- COMMENT: 903cb37e16a1d237 100 /IubNdTvlTojm9E/Y+WYYua9SJ8 (comment: CHECK 54.78% of wild-type spore viability, epistatic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 101 nj/CNLuGGraTaoXi/D+C472Vj+c (comment: CHECK 28.85% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, epistatic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 102 /JlpWpZ9SL3yKvFmfM7q0Ri7v1U (comment: CHECK 3.55% of wild-type recombination assayed between ade6-3083 and ade6-469) (Fig.6A, Table S6) ------- COMMENT: 903cb37e16a1d237 103 WUNGzpRE0kaTyJruDyMjWc9uI10 (comment: CHECK 119.91% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim, epistatic relationship) (Fig. 5A, Table S6) ------- COMMENT: 903cb37e16a1d237 104 4GkM+Ih+yDmZFR2qHjJkJa3ioNY (comment: CHECK 55.62% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, partial rescue from rad55Δ levels) (Table S6) ------- COMMENT: 903cb37e16a1d237 105 0lu1PsDlPG1wMTwumaY/rUtf8v4 (comment: CHECK 35.42% of wild-type recombination assayed between ade6-3083 and ade6-469, epistatic relationship) (Fig.6B, Table S6) ------- COMMENT: 903cb37e16a1d237 106 I4DTxJIATaRvxMDJKhZsPdv6Npo (comment: CHECK 111.64% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim, epistatic relationship) (Fig. 5B, Table S6) ------- COMMENT: 903cb37e16a1d237 107 ha1BdgHVn9Vs9bAXHTDc2HGEFRw (comment: CHECK 53.76% of wild-type spore viability, epistatic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 108 raQfcNg08E9o5Se0ocFE6/7CDBs (comment: CHECK 15.19% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, epistatic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 109 2SDCparp4OZOsk56RpE+XuNMdS4 (comment: CHECK 8.87% of wild-type recombination assayed between ade6-3083 and ade6-469, epistatic relationship) (Fig.6B, Table S6) ------- COMMENT: 903cb37e16a1d237 110 GKWVWvKXtaG+FBDOhxaM7yfm98c (comment: CHECK 127.22% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim, synergistic relationship) (Fig. 5B, Table S6) ------- COMMENT: 903cb37e16a1d237 111 saGwpnc2KnGgFB5msS5DJCfe/F8 (comment: CHECK 3.68% of wild-type spore viability, synergistic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 112 u5prTMgh12MOwyrkjQtvgqBdcyQ (comment: CHECK 15.53% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, epistatic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 113 SUN52/uYXFl8Y378egbct8qZat0 (comment: CHECK 12.19% of wild-type recombination assayed between ade6-3083 and ade6-469, epistatic relationship) (Fig.6B, Table S6) ------- COMMENT: 903cb37e16a1d237 114 a0I7vdiA3wSfzpvHH7V4LYjbG2U (comment: CHECK 136.75% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim, synergistic relationship) (Fig. 5B, Table S6) ------- COMMENT: 903cb37e16a1d237 115 xy2Fe29+ooyv9KCXR9haaVmZJzg (comment: CHECK 4.11% of wild-type spore viability) (Table S6) ------- COMMENT: 903cb37e16a1d237 116 cEcCXcOhkFnM4wP4qk4cD9Ki9TA (comment: CHECK 69.41% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, epistatic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 117 8rf9/fIormV82CXOb3hEf9JPbf0 (comment: CHECK 36.88% of wild-type recombination assayed between ade6-3083 and ade6-469, epistatic relationship) (Fig.6C, Table S6) ------- COMMENT: 903cb37e16a1d237 118 IdwPk0XCIq6QBwvoG2+iuiraeW0 (comment: CHECK 101.33% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim; higher than rlp1Δ-7, lower than fml1Δ, skewed recombinant categories) (Figs. 5C, 7A, Table S6) ------- COMMENT: 903cb37e16a1d237 120 hehUx7nn6wr1TjhNAaNZxvwy3ls (comment: CHECK 46.52% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, epistatic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 121 sMHTteer3YQzFpwc+M1i9ftfUS0 (comment: CHECK 52.08% of wild-type recombination assayed between ade6-3083 and ade6-469, epistatic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 122 6j3gKaFHFdXnYQ5qUyRGfQoE7ZE (comment: CHECK 102.17% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim, higher than rdl1Δ-25, lower than fml1Δ, skewed recombinant categories) (Fig. S3, Table S6) ------- COMMENT: 903cb37e16a1d237 123 4n0oJySVEWQa6TZu/dbFZx+T02Y (comment: CHECK 73.46% of wild-type spore viability, epistatic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 124 6TszM9T7TuZZwBjDDTZIfJhpgwk (comment: CHECK 32.13% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, epistatic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 125 E8f71BrS/xUVTJKQokADlkoP6vU (comment: CHECK 8.33% of wild-type recombination assayed between ade6-3083 and ade6-469, epistatic relationship) (Fig.6C, Table S6) ------- COMMENT: 903cb37e16a1d237 126 QOHKm51x2OpSepUAS86t2+YrBR8 (comment: CHECK 116.19% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim, synergistic relationship) (Fig. 5C, Table S6) ------- COMMENT: 903cb37e16a1d237 127 1W/8GkzrzUkgyal4CnQDnW/BbuA (comment: CHECK 37.75% of wild-type spore viability, epistatic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 128 drhTGDkXSpG6s8wns927vqncYUI (comment: CHECK 59.57% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, epistatic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 129 E+SUsxALQV+oiM5o7NHGUiq0wxQ (comment: CHECK 7.33% of wild-type recombination assayed between ade6-3083 and ade6-469, epistatic relationship) (Fig.6C, Table S6) ------- COMMENT: 903cb37e16a1d237 130 eAIT7m7PZIuHsZeT3sXd2vqYaiI (comment: CHECK 130.25% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim, synergistic relationship) (Fig. 5C, Table S6) ------- COMMENT: 903cb37e16a1d237 131 I66cm/cVOb2amK348NMEJJDXxho (comment: CHECK 21.45% of wild-type spore viability, synergistic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 132 nT60kCIXdwXeaxim4ISQQhq2Huc (comment: CHECK 49.8% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, epistatic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 133 el8lTSe7055etJ2b1IZMSQetmCI (comment: CHECK 27.08% of wild-type recombination assayed between ade6-3083 and ade6-469, epistatic relationship) (Fig.6D, Table S6) ------- COMMENT: 903cb37e16a1d237 134 V2/GkZ81ypqxwPNEMF8PeEZ82gA (comment: CHECK 122.65% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim, epistatic relationship) (Fig. 5D, Table S6) ------- COMMENT: 903cb37e16a1d237 135 ZzoDEMjIM15+2x57Zq2xEepnqsk (comment: CHECK 91.68% of wild-type spore viability, epistatic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 136 WTdrK2kkMrT3LZMCdgrfj6V4ROY (comment: CHECK 38.89% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, epistatic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 137 LXEdcRoI+gt8tAa6yh2yOhArfpE (comment: CHECK 10.34% of wild-type recombination assayed between ade6-3083 and ade6-469, epistatic relationship) (Fig.6D, Table S6) ------- COMMENT: 903cb37e16a1d237 138 MaU5vlNt2tX+MaMoVFMumCzE1hI (comment: CHECK 101.4% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim; skewed recombinant categories) (Fig. 5D, Table S6) ------- COMMENT: 903cb37e16a1d237 139 GTEDKhkLEKNO0ej9REec7uT/GGw (comment: CHECK 21.46% of wild-type spore viability, synergistic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 140 jz13Mg9bM/biKXU2vRxQZbeFE1M (comment: CHECK 45.45% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, epistatic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 141 uRB6Q/fCWKOXn6snzFsVya+DQZc (comment: CHECK 15.59% of wild-type recombination assayed between ade6-3083 and ade6-469) (Fig.6D, Table S6) ------- COMMENT: 903cb37e16a1d237 142 S78L4nP3WowfL3FqsKOMR4Pr0Ng (comment: CHECK 114.54% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim; skewed recombinant categories) (Fig. 5D, Table S6) ------- COMMENT: 903cb37e16a1d237 143 gt3HO5GjZxzWoloabQpI3ygWo2k (comment: CHECK 20.24% of wild-type spore viability) (Table S6) ------- COMMENT: 903cb37e16a1d237 144 WPSzkvQkSeF8HqBZnnO9mR3QXss (comment: CHECK 55.49% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, epistatic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 145 pdQACsWNVOrxjddE14kReXWSYlg (comment: CHECK 37.89% of wild-type recombination assayed between ade6-3083 and ade6-469, similar to dmc1Δ-12 and rlp1Δ single mutants, but higher than dmc1Δ-12 rlp1Δ double mutant) (Fig.6E, Table S6) ------- COMMENT: 903cb37e16a1d237 146 uXW7WtL/mrF6MJm2SGYUIbZPWNU (comment: CHECK 79.92% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim; skewed recombinant categories) (Figs. 5E, 7A, Table S6) ------- COMMENT: 903cb37e16a1d237 147 Go6M3Tb8oiSalqf/+UjCqKSF43k (comment: CHECK 7.8% of wild-type spore viability, synergistic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 148 recObfp2pmYvAihvaDbDdX72pzI (comment: CHECK 30.92% of wild-type recombination assayed between ura4+-aim2 and his3+-aim, epistatic relationship) (Table S6) ------- COMMENT: 903cb37e16a1d237 149 IDfRvNDm0iN/e48VY9lI+DjwTFg (comment: CHECK 9.88% of wild-type recombination assayed between ade6-3083 and ade6-469, epistatic relationship) (Fig.6E, Table S6) ------- COMMENT: 903cb37e16a1d237 150 Hg2I2/SkQzLTzCfne9HVAcDSfCw (comment: CHECK 101.76% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim; skewed recombinant categories) (Figs. 5E, 7A, Table S6) ------- COMMENT: 903cb37e16a1d237 151 YQa4lB+58k45U6+3wAq1f17Rk+w (comment: CHECK 31.18% of wild-type spore viability) (Table S6) ------- COMMENT: 903cb37e16a1d237 152 LoXrRu2qd7Ig/+jkLwwcBNFePJ0 (comment: CHECK 20.15% of wild-type recombination assayed between ura4+-aim2 and his3+-aim) (Table S6) ------- COMMENT: 903cb37e16a1d237 153 xEuaQj3ZUMy7BDOXD9gsgZhf0/8 (comment: CHECK 6.87% of wild-type recombination assayed between ade6-3083 and ade6-469, epistatic relationship) (Fig.6E, Table S6) ------- COMMENT: 903cb37e16a1d237 154 JpOCetDKh8F1VS30u2ibh0yGGV8 (comment: CHECK 111.2% of wild-type recombination assayed between ura4+-aim2 - ade6-3083 and ade6-469 - his3+-aim; skewed recombinant classes) (Figs. 5E, 7A, Table S6) ------- COMMENT: 903cb37e16a1d237 155 T3OMOh8h0+tnALj7F1gt8PMNXRg (comment: CHECK 13.87% of wild-type spore viability) (Table S6) ------- COMMENT: 904ada6c1059d0f1 1 YBC7hhpyQU6ZVwD2USouFsosS8c (comment: (transient expression)) most surviving cells generated diploid or tetraploid clones/ if rum was derepressed for only a short period, most surviving cells generated diploid or tetraploid clones, as would be expected if there were complete rounds of DNA replication. Fig1b ------- COMMENT: 904ada6c1059d0f1 2 AZNLi2+GWij0ZQAWQhaVkAr7JRQ Fig1A Overreplicating haploid cells become highly enlarged (Fig. la, left), ------- COMMENT: 904ada6c1059d0f1 3 ZgPGknJRMq+ArrSVE64TONESBXA Table 1 (comment: CHECK increased cell size required for the G1/S transition.) ------- COMMENT: 904ada6c1059d0f1 4 gxEvW0K1T1cegCrQHpT9A3J5IKA (comment: CHECK in vitro assay) data not shown ------- COMMENT: 904ada6c1059d0f1 5 UjnwhJzVRznLxSqab/Ukk8GMR2o Fig2B. (comment: prestart) ------- COMMENT: 904ada6c1059d0f1 7 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 904ada6c1059d0f1 9 rjx2unJ5A7BMqnAXqbBbuC/SKAM data not shown, cells become arrest in G2 ------- COMMENT: 904ada6c1059d0f1 10 f1GzbyY5VORHWGuwulOiSJ4fUBQ Fig 4A, B. In cdc10-129 rum1 delta strain cells fail to activate the DNA replication checkpoint in the absence of DNA replication and ------- COMMENT: 904ada6c1059d0f1 11 PZKNPjzeiR2fbYeY5Ak7Z/PIjEU (comment: negative regulation/(I now think this is the same as G1 checkpoint).) expression results in a small delay of S phase onset until cells attain a higher mass, suggesting the rumr gene product may act as a transient inhibitor of progres- sion through GI into S phase. The appearance of a small popu- lation of IC cells at 16 h is consistent with this interpretation (Fig. lb). ------- COMMENT: 904ada6c1059d0f1 12 bi5+e4MJuKsjLUpuhUGDkxuvDAQ Fig1B (comment: they give a pulse of rum1) ------- COMMENT: 904ada6c1059d0f1 13 sZctA2lyYQPRQ34lNdy1SdX/bVY At both temperatures the DNA content per cell continued to increase, demonstrating that the G2-arrested cells were able to undergo further rounds of S phase (Fig. 2c and d, right) . ------- COMMENT: 904ada6c1059d0f1 14 nqGpvTQ/N+o6VJ09xwSPOs6cm8s Fig3a ------- COMMENT: 904ada6c1059d0f1 15 pPJkRMh2R5SETzz8AscaCvIyHWI Fig. 4a elongated cell, cells do not replicate DNA (never enter S phase), but keep growing ------- COMMENT: 904ada6c1059d0f1 16 fujxw5sI6QH2R+dKfdnBuxlfR2A xpression results in a small delay of S phase onset until cells attain a higher mass, suggesting the rumr gene product may act as a transient inhibitor of progres- sion through GI into S phase. The appearance of a small popu- lation of IC cells at 16 h is consistent with this interpretation (Fig. lb). ------- COMMENT: 904ada6c1059d0f1 17 fujxw5sI6QH2R+dKfdnBuxlfR2A xpression results in a small delay of S phase onset until cells attain a higher mass, suggesting the rumr gene product may act as a transient inhibitor of progres- sion through GI into S phase. The appearance of a small popu- lation of IC cells at 16 h is consistent with this interpretation (Fig. lb). ------- COMMENT: 904ada6c1059d0f1 18 xJtI7ELMDHAnIlAISFviS4/Nqvs Fig 4d, f. In cdc10-129 rum1 delta strain cells fail to activate the DNA replication checkpoint in the absence of rum1 and cells proceed into mitosis in the absence of DNA replication and enter mitosis with reduced DNA content proce of DNA replication and ------- COMMENT: 904ada6c1059d0f1 19 IL84HVloaiPFihtHSIt+7pBdo/k Fig 4E G. In cdc10-129 rum1 delta strain cells at 36 fail to activate the DNA replication checkpoint in the absence of rum1 and cells proceed into mitosis in the presence of HU and enter mitosis with reduced DNA content ------- COMMENT: 9075dee80f064d3a 10 ej7s5n3gU0nNYhRYCC9b7Y5H9pY (comment: CHECK taf73 does not substitute for taf5) ------- COMMENT: 9079e758e75e287f 1 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 9079e758e75e287f 2 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 9079e758e75e287f 3 OZxRwThKT0crpw346BheciIuaew Figure 1C In contrast, in the absence of the bouquet, the duplicated SPBs often fail to separate. Indeed, 75.5% of bqt1Δ cells with defective meiosis show problems in SPB separation at MI ------- COMMENT: 9079e758e75e287f 4 MU4uHAO59XT8+JMo4nlGZSPbsqA Figure 1C In contrast, in the absence of the bouquet, the duplicated SPBs often fail to separate . Indeed, 75.5% of bqt1Δ cells with defective meiosis show problems in SPB separation at MI ------- COMMENT: 9079e758e75e287f 5 iAcbpl1ew+nFwb8AiAqOLZxpvN8 Figure 1D ------- COMMENT: 9079e758e75e287f 6 iAcbpl1ew+nFwb8AiAqOLZxpvN8 Figure 1D ------- COMMENT: 9079e758e75e287f 7 9qepF10SXAgw3gr/14QMoWI8cYQ Figure 1E In contrast, Alp4 localization is defective (ie one or both SPB signals lack any detectable Alp4 colocalization at MI onset) in 59% of bqt1Δ meiocytes (n= 100, p<0.01) from the onset of MI onwards, ------- COMMENT: 9079e758e75e287f 8 nbffKiLJZHfuiCUCaP1eIAHN5Ik Figure 1E and all (n=50, p<0.01) those SPBs failing to recruit Alp4 show SPB separation problems and failed spindle nucleation (Figure 1E). ------- COMMENT: 9079e758e75e287f 10 ELp/4rfqN+DaXGb3+iiKpuJCbNE (comment: Figure) In 100% of the bqt1Δ cells that show monopolar spindles (n=11), those spindles are nucleated specifically from the old SPB (Figure S1I). Hence, failed spindle nucleation in the absence of the bouquet is specific to the new SPB. ------- COMMENT: 9079e758e75e287f 12 7dFYGy5mINchSPf+KjgDFDD6QP8 Figure 1C, Figure 1F, 1G. We previously observed a tendency for the SPB to dissociate from the NE just prior to meiotic spindle formation in the bqt1Δ setting (Fennell et al., 2015; Tomita and Cooper, 2007); indeed, SPBs showing problems in separation typically appear to dislodge into the cytoplasm (Figure 1C, yellow arrowheads ------- COMMENT: 9079e758e75e287f 13 FAqoHipqt+tFsE+DqieqtDXJRg8 Figure 1 ------- COMMENT: 9079e758e75e287f 14 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 9079e758e75e287f 15 UIv506AdFFBg8sXygkJ7nMdoHmI fig2c ------- COMMENT: 9079e758e75e287f 16 UIv506AdFFBg8sXygkJ7nMdoHmI fig2c ------- COMMENT: 9079e758e75e287f 17 UIv506AdFFBg8sXygkJ7nMdoHmI fig2c ------- COMMENT: 9079e758e75e287f 18 UIv506AdFFBg8sXygkJ7nMdoHmI fig2c ------- COMMENT: 9079e758e75e287f 19 tpSwAUFFfiAJs6TVEDKOZkgQR5Y Figure 3A. Sad1.2-GFP remains stably associated with the SPB throughout interphase, in contrast to Sad1.1-GFP, which is destabilized at 36°C ------- COMMENT: 9079e758e75e287f 21 tpSwAUFFfiAJs6TVEDKOZkgQR5Y Figure 3A. Sad1.2-GFP remains stably associated with the SPB throughout interphase, in contrast to Sad1.1-GFP, which is destabilized at 36°C ------- COMMENT: 9079e758e75e287f 22 7CjHfwj9Bpw3XG9Zd32G3N5+vmc Figures 3B–C, S4A ------- COMMENT: 9079e758e75e287f 23 7CjHfwj9Bpw3XG9Zd32G3N5+vmc Figures 3B–C, S4A ------- COMMENT: 9079e758e75e287f 24 pwYo4qq3zpJvUEZsuqNj7EY7jzA Figure S4B ------- COMMENT: 9079e758e75e287f 25 pwYo4qq3zpJvUEZsuqNj7EY7jzA Figure S4B ------- COMMENT: 9079e758e75e287f 26 PbaH6nZsUDq1SnkLn65OoNp1VHw fig 3DE sad1.2 cells often show extra Mis6-GFP foci unassociated with the SPB, even at permissive temperature ------- COMMENT: 9079e758e75e287f 27 3w1EtA3b0PKVYmmEuc2D5mPkgFY (Figure 3D, E) At restrictive temperature, a population of sad1.2 cells emerges in which all three centromeres are clearly dissociated from the SPB ------- COMMENT: 9079e758e75e287f 28 KNyy8axuxhy+fCoJXGv5k2dpVdQ fig 3D, E ------- COMMENT: 9079e758e75e287f 29 oinmh1+D/RUF0ZDT82BGcN7gPco (Figure 3D, E) ------- COMMENT: 9079e758e75e287f 30 fEPPdd8vcIyAT29IadgAwvAhaOE (Figure 4B) ------- COMMENT: 9079e758e75e287f 31 dCjXSxMfsf7K0xUpFf2teRGmnvw (Figures 4D–E, S5B, S5D) sad1.2 cells exhibiting total centromere dissociation not only fail to insert but also appear to separate from the NE, dislodging into the cytoplasm ------- COMMENT: 9079e758e75e287f 32 UIaVxpnj+1eTshZdfli78HnlASU fig 5C ------- COMMENT: 9079e758e75e287f 33 GFvYmM9Pw89BQAQC0bRAgz1MmvQ Figure 5B. (comment: centromeres are also released from LINC in bouquet-defective cells) ------- COMMENT: 9079e758e75e287f 34 hmOmeK4DIXkToAjA9OPexwUxLQw Figure 5C. spindle formation occurs normally at both MI and MII in sad1.2 meiosis ------- COMMENT: 9079e758e75e287f 35 5/bTljEdhnePFKMcWRGfHcYQpgk (comment: CHECK abolished) ------- COMMENT: 9079e758e75e287f 37 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: 9079e758e75e287f 38 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: 9079e758e75e287f 39 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 90ac205089d49c8b 12 XU6w6+OcYvrGbiFZV7PLcIdlPdk (comment: CHECK meiosis II) ------- COMMENT: 90e7f1df3e7c70af 1 nnuWhrFU7jaQ2CsXT4A21I2oLbM fig 3a ------- COMMENT: 90e7f1df3e7c70af 3 sx+0SFokrvLQ0K5C6ktpPYZzmtc (comment: CHECK (with decreased total volume -. new term requested)) Throughout the period of glucose starvation, mito- chondria in dnm1􏰇 cells did not appear to fragment but shrunk over time (Fig. 3A). mitochondrion numbers remained largely unchanged during glucose starvation (Fig. 3B) ------- COMMENT: 90e7f1df3e7c70af 5 jc/ZDB7Nq+yNz/h3c2bU4aWYEvk figure 4c ------- COMMENT: 90e7f1df3e7c70af 6 jc/ZDB7Nq+yNz/h3c2bU4aWYEvk figure 4c ------- COMMENT: 90e7f1df3e7c70af 7 /VwjLgC6NAd+7zhgRwG0v8HvrA0 figure 4a, b ------- COMMENT: 90e7f1df3e7c70af 8 /VwjLgC6NAd+7zhgRwG0v8HvrA0 figure 4a, b ------- COMMENT: 90e7f1df3e7c70af 9 gRZB/YpvIHkwr9r0jOi8SE7FLbw As shown in Fig. 5A, the changes in mitochondrial morphology were similar within 40 min of glucose starvation in the three mutant and WT cells ------- COMMENT: 90e7f1df3e7c70af 12 NmCf+ND01GSpjYKSWq6pMJcVMkg Indeed, no noticeable change in mitochondrial morphology or altered mitochondrion numbers were found in the three mutant cells cultured in glucose-rich EMM (Fig. 5, C and D). ------- COMMENT: 90e7f1df3e7c70af 15 rhksm65CKwvVwYamaDVwfyEciHA As shown in Fig. 6, A and B, ROS production under glucose starvation was reduced, but not abolished, in the absence of Dnm1 because only 􏰆25% of dnm1􏰇 cells were DCDHF-DA– positive after glucose starvation. ------- COMMENT: 914a897d725c3b1c 3 8LBoeF+Wq7VMqJg7oHceMrR/2vo Consistent with their observed structural roles, charge reversal mutations Arg616Glu, Lys617Glu, and Lys619Glu all abolished Crb2–BRCT2 interaction with the peptide (Fig. 3A), ------- COMMENT: 914a897d725c3b1c 4 Ks5iwb76GePqN91Svp9tUIxRY+Y (comment: CHECK ") ------- COMMENT: 914a897d725c3b1c 5 8LBoeF+Wq7VMqJg7oHceMrR/2vo Consistent with their observed structural roles, charge reversal mutations Arg616Glu, Lys617Glu, and Lys619Glu all abolished Crb2–BRCT2 interaction with the peptide (Fig. 3A), ------- COMMENT: 914a897d725c3b1c 6 8LBoeF+Wq7VMqJg7oHceMrR/2vo Consistent with their observed structural roles, charge reversal mutations Arg616Glu, Lys617Glu, and Lys619Glu all abolished Crb2–BRCT2 interaction with the peptide (Fig. 3A), ------- COMMENT: 914a897d725c3b1c 7 Ks5iwb76GePqN91Svp9tUIxRY+Y (comment: CHECK ") ------- COMMENT: 914a897d725c3b1c 8 Ks5iwb76GePqN91Svp9tUIxRY+Y (comment: CHECK ") ------- COMMENT: 914a897d725c3b1c 9 8LBoeF+Wq7VMqJg7oHceMrR/2vo Consistent with their observed structural roles, charge reversal mutations Arg616Glu, Lys617Glu, and Lys619Glu all abolished Crb2–BRCT2 interaction with the peptide (Fig. 3A), ------- COMMENT: 914a897d725c3b1c 10 8LBoeF+Wq7VMqJg7oHceMrR/2vo Consistent with their observed structural roles, charge reversal mutations Arg616Glu, Lys617Glu, and Lys619Glu all abolished Crb2–BRCT2 interaction with the peptide (Fig. 3A), ------- COMMENT: 914a897d725c3b1c 11 8LBoeF+Wq7VMqJg7oHceMrR/2vo Consistent with their observed structural roles, charge reversal mutations Arg616Glu, Lys617Glu, and Lys619Glu all abolished Crb2–BRCT2 interaction with the peptide (Fig. 3A), ------- COMMENT: 914a897d725c3b1c 12 0U7XqVATtPJOlksUfvoiN/15fBI the Ser666Arg and Cys663Arg mutants ran as monomeric species in gel filtration, indicative of disruption of their dimerization ------- COMMENT: 914a897d725c3b1c 13 0U7XqVATtPJOlksUfvoiN/15fBI the Ser666Arg and Cys663Arg mutants ran as monomeric species in gel filtration, indicative of disruption of their dimerization ------- COMMENT: 914a897d725c3b1c 14 cosfVR8+rUfPp01JsEOB+spUqNI Conversely, mutations disrupting dimerization did not disrupt  -H2A.1 binding (Fig. 3C). ------- COMMENT: 914a897d725c3b1c 15 cosfVR8+rUfPp01JsEOB+spUqNI Conversely, mutations disrupting dimerization did not disrupt  -H2A.1 binding (Fig. 3C). ------- COMMENT: 914a897d725c3b1c 16 cosfVR8+rUfPp01JsEOB+spUqNI Conversely, mutations disrupting dimerization did not disrupt  -H2A.1 binding (Fig. 3C). ------- COMMENT: 914a897d725c3b1c 17 cosfVR8+rUfPp01JsEOB+spUqNI Conversely, mutations disrupting dimerization did not disrupt  -H2A.1 binding (Fig. 3C). ------- COMMENT: 914a897d725c3b1c 18 ePp0YOfOTv2D+J9pccopDWRT6v0 fig 4a ------- COMMENT: 914a897d725c3b1c 19 ePp0YOfOTv2D+J9pccopDWRT6v0 fig 4a ------- COMMENT: 914a897d725c3b1c 20 ePp0YOfOTv2D+J9pccopDWRT6v0 fig 4a ------- COMMENT: 914a897d725c3b1c 21 ePp0YOfOTv2D+J9pccopDWRT6v0 fig 4a ------- COMMENT: 914a897d725c3b1c 22 ePp0YOfOTv2D+J9pccopDWRT6v0 fig 4a ------- COMMENT: 914a897d725c3b1c 23 ePp0YOfOTv2D+J9pccopDWRT6v0 fig 4a ------- COMMENT: 914a897d725c3b1c 24 ePp0YOfOTv2D+J9pccopDWRT6v0 fig 4a ------- COMMENT: 914a897d725c3b1c 25 ePp0YOfOTv2D+J9pccopDWRT6v0 fig 4a ------- COMMENT: 914a897d725c3b1c 26 ePp0YOfOTv2D+J9pccopDWRT6v0 fig 4a ------- COMMENT: 914a897d725c3b1c 27 ePp0YOfOTv2D+J9pccopDWRT6v0 fig 4a ------- COMMENT: 9178dbaf766a122a 1 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 9178dbaf766a122a 2 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 9178dbaf766a122a 3 oSiXUWiPOABFqHFVQxLfu3X60hM fig 1 (comment: nuclear) ------- COMMENT: 9178dbaf766a122a 4 XPnM32shLX68Eo2rR66Yrm1uKgQ fig 1c ------- COMMENT: 9178dbaf766a122a 5 FaLWaEQ3IAs+bjT9xTnBgKoQbFk fig 2e suggesting that Vgl1 might escort RNA from ER-associated polyribosomes to the cytosol under thermal stress. ------- COMMENT: 9178dbaf766a122a 6 mrzFP8HqSEhkWH0BkIHE+1FHffk fig 5e ------- COMMENT: 9178dbaf766a122a 8 fYqQOpzikopc+3AWAEPEUkYII7w figure 4 ------- COMMENT: 9178dbaf766a122a 9 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: 9178dbaf766a122a 10 Jlk1V51+Ro3/iw8g0EtgXjlKa3U fig 5a ------- COMMENT: 9178dbaf766a122a 11 8N5pCuJDvQ4tU27Tb2MYBaI8aOw fig 5b ------- COMMENT: 9178dbaf766a122a 12 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: 9178dbaf766a122a 15 etqTRpW8rN0qMBvhiHMFy/NG+Vo fig 6D ------- COMMENT: 9178dbaf766a122a 16 srNPEOHUR5maeOFJvV9hJaeBsr4 fig 6c ------- COMMENT: 9178dbaf766a122a 17 BwFGbt+AOrQ+ChKB9KLeEsmu598 Figure 6E) ------- COMMENT: 9178dbaf766a122a 18 LHYPi7f4v765/FZ3eTZ7K3ZwOqA (Figure 7A) ------- COMMENT: 9178dbaf766a122a 19 qRfErBu0efCkJmXI29Ug1GX1SOE (Figure 7B) ------- COMMENT: 9178dbaf766a122a 20 fVtielsr+m1x6ypFbIJ+YNj5v8Y Figure 8A and B (comment: (fairly similar expression orofiles)) ------- COMMENT: 9178dbaf766a122a 21 8N5pCuJDvQ4tU27Tb2MYBaI8aOw fig 5b ------- COMMENT: 9178dbaf766a122a 22 PKKClEb5YvfUv3yoSQYBgiC/qvE figure 6a ------- COMMENT: 9197675c3ba9f5d1 60 QO5Z27AlwemJyLf9LmONdHkhXj4 (comment: mRNA co-immunoprecipitated with ribosomes) ------- COMMENT: 9197a3d2cbe7051e 2 j1Y39RlIVdtqbMP0OKaNBnydhes (comment: physiologically relevant?) ------- COMMENT: 9197a3d2cbe7051e 4 j1Y39RlIVdtqbMP0OKaNBnydhes (comment: physiologically relevant?) ------- COMMENT: 91da8e4c4e7b6d48 1 FHwGyCagIDkIHMKU98zQzEk3ENE cells divide at 51% of control cell length at division ------- COMMENT: 91da8e4c4e7b6d48 2 z2/ghUEJ7EzR1CjDX6hhh2/YLIo small cell size at division is partially suppressed in the presence of sup3-5 an opal nonsense suppressor mutation in the sup3 tRNA gene. Cells divide at 89% of control cell length at division so are not really normal size ------- COMMENT: 91da8e4c4e7b6d48 3 7k7VboLjb80yWDoiJDLavT4apIw cells septate at 87% of wild type diploid length ------- COMMENT: 91da8e4c4e7b6d48 4 sxshwsiJzW6FfTxqUMCiUSTM3d8 cells septate at 77% of wild type diploid length ------- COMMENT: 91da8e4c4e7b6d48 5 ljInuZNUUZ6mZikAb5XAOXKsAEA cells septate at 85% of wild type diploid length ------- COMMENT: 91da8e4c4e7b6d48 6 lkVb4CuFhtbk/1AYO+cVQpbMxfY cells septate at 82% of wild type diploid length ------- COMMENT: 91da8e4c4e7b6d48 7 FiPXmVZ+OnfqnW1D2uGeKpM4A6U cells septate at 81% of wild type diploid length ------- COMMENT: 91da8e4c4e7b6d48 8 a6G5ORPWHluRHxO7nne1tQE0J0s cells septate at 52% of wild type diploid length ------- COMMENT: 91da8e4c4e7b6d48 9 iDoKs8bK7BfUmiXIrqrHojDFBSs cells septate at 58% of wild type diploid length ------- COMMENT: 91da8e4c4e7b6d48 10 IZJJuRG2/204uWW9lhj+qQd8VZ0 cells septate at 54% of wild type diploid length ------- COMMENT: 91da8e4c4e7b6d48 11 iDoKs8bK7BfUmiXIrqrHojDFBSs cells septate at 58% of wild type diploid length ------- COMMENT: 91da8e4c4e7b6d48 12 4QSPeC82eCYNsedd0Kw8tevHQoo cells septate at 56% of wild type diploid length ------- COMMENT: 91da8e4c4e7b6d48 13 6gcKzFVozjqRHo9EL0CFLX0Ve5E (comment: cdc2-1w was previously called wee2-1) ------- COMMENT: 91da8e4c4e7b6d48 14 UOmUv42w0ytPu+0NgAVxJglW10c cells divide at 22.4µm at 25°C ------- COMMENT: 91da8e4c4e7b6d48 15 XUc9NOqfJOdTh+TcQAYIH0X/Z3s cells divide at 10.2µm at 25°C ------- COMMENT: 91da8e4c4e7b6d48 16 PCSgQ5Ip1c1bVuZOgksJOTP4wag (comment: This mutation is probably allelic with cdc2-56. Cells divide at 10.0 µm. I think it is useful to have it annotated as it is in old literature and people may wonder what it is) ------- COMMENT: 91da8e4c4e7b6d48 17 qEZz8rNqd/CfEZJ2Kibz/NXoxyE cells divide at 16.7µm at 25°C ------- COMMENT: 91da8e4c4e7b6d48 18 92tlsQqXx9KOBBOZRbBe8nv0+aI Cells divide at 65% of wild type diploid cell length ------- COMMENT: 91da8e4c4e7b6d48 19 FHP7DqqwTK7M3u1xPvxRCftsAzo cells divide at 56% of the size at division of wild type diploids ------- COMMENT: 91da8e4c4e7b6d48 20 O4AY6tUIpNhindwNF2Xwdg1CGX0 cells divide at 82% of wild diploid size at division ------- COMMENT: 91da8e4c4e7b6d48 22 Yt3EnuPBJ1pvdRZGSWqZs8T/BDg cells divide at 7% longer than wild diploid cells at division ------- COMMENT: 91da8e4c4e7b6d48 24 gtqEMJMngJC0SKtISJF6DRaGGK8 cells divide at 12% longer than wild diploid cells at division ------- COMMENT: 91da8e4c4e7b6d48 26 4lgt2Rcf8JRDINnTdkeaGiv9Agg cells divide at 10% longer than wild diploid cells at division ------- COMMENT: 91da8e4c4e7b6d48 27 VaVHlfxXnaDyjElu9bCyXEElp9s cells divide at 11% longer than wild diploid cells at division ------- COMMENT: 91da8e4c4e7b6d48 28 VaVHlfxXnaDyjElu9bCyXEElp9s cells divide at 11% longer than wild diploid cells at division ------- COMMENT: 91da8e4c4e7b6d48 29 /fdnRrgztTLzYRs9FvLDXdnVoaE cells divide at 6% longer than wild diploid cells at division ------- COMMENT: 91da8e4c4e7b6d48 30 4lgt2Rcf8JRDINnTdkeaGiv9Agg cells divide at 10% longer than wild diploid cells at division ------- COMMENT: 91da8e4c4e7b6d48 31 p8IQIdF92xca9T/4BuGNBS12umc cells divide at 8% longer than wild diploid cells at division ------- COMMENT: 91da8e4c4e7b6d48 32 8VVheh/srUe7rmrAbnD78OxmR7k cells divide at 9% longer than wild diploid cells at division ------- COMMENT: 91da8e4c4e7b6d48 33 mxSaWXURFty+wlI/1YEaNgn/v+o cells divide at 75% of wild type diploid cell length at division at 25°C ------- COMMENT: 91fbedafa14f14da 1 xBwJNiQ7wvH2QtheFxpIafkQhd4 Assayed using S. japonicus rop1 in vitro. Fig. 4 ------- COMMENT: 91fbedafa14f14da 4 fW30aVhWks941NA/lHoMBVjgieY Fig. 1D and E ------- COMMENT: 91fbedafa14f14da 5 vsnTBDdTc5piO0MaQ4C8xYGaxLU Assayed using S. japonicus yop1 in vitro. Fig. 4 ------- COMMENT: 91fbedafa14f14da 7 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 91fbedafa14f14da 8 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 91fbedafa14f14da 13 Zno40JIIfMde9u5cNVEbdVzh+iw Fig. 1B and C ------- COMMENT: 91fbedafa14f14da 14 Zno40JIIfMde9u5cNVEbdVzh+iw Fig. 1B and C ------- COMMENT: 91fbedafa14f14da 15 Zno40JIIfMde9u5cNVEbdVzh+iw Fig. 1B and C ------- COMMENT: 91fbedafa14f14da 16 Zno40JIIfMde9u5cNVEbdVzh+iw Fig. 1B and C ------- COMMENT: 91fbedafa14f14da 17 Zno40JIIfMde9u5cNVEbdVzh+iw Fig. 1B and C ------- COMMENT: 91fbedafa14f14da 18 Zno40JIIfMde9u5cNVEbdVzh+iw Fig. 1B and C ------- COMMENT: 91fbedafa14f14da 19 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 91fbedafa14f14da 20 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 91fbedafa14f14da 21 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 91fbedafa14f14da 22 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 91fbedafa14f14da 23 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 91fbedafa14f14da 24 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 91fbedafa14f14da 25 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 91fbedafa14f14da 26 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 91fbedafa14f14da 27 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 91fbedafa14f14da 28 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 91fbedafa14f14da 29 FzDOQ+uADGjO1EkfbBvT7AVQpPM Fig. S2D ------- COMMENT: 91fbedafa14f14da 30 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: 91fbedafa14f14da 31 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: 91fbedafa14f14da 32 hjAVLgiVd8IEhJQDMnaOhYI2WA4 Fig. S2G and H ------- COMMENT: 91fbedafa14f14da 33 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 91fbedafa14f14da 34 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 91fbedafa14f14da 35 hjAVLgiVd8IEhJQDMnaOhYI2WA4 Fig. S2G and H ------- COMMENT: 91fbedafa14f14da 36 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 91fbedafa14f14da 37 MQHdVxWEBXLXSvso6B2z8ZaGNUE Fig. 3G ------- COMMENT: 91fbedafa14f14da 38 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 91fbedafa14f14da 39 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 91fbedafa14f14da 40 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 91fbedafa14f14da 41 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 91fbedafa14f14da 42 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 91fbedafa14f14da 43 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 91fbedafa14f14da 44 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 91fbedafa14f14da 45 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 91fbedafa14f14da 46 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 91fbedafa14f14da 47 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 91fbedafa14f14da 48 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 91fbedafa14f14da 49 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 92141eb3ba63c234 1 LR5JVp8U1dJ2A8C5leLYcMz/O0Q In the absence of Rad52, newly replicated strands are extensively resected at dysfunctional replication forks thus generating mitotic sister chromatid bridging. ------- COMMENT: 92141eb3ba63c234 3 ine2Q0aXA4Gbdj/HYBKiOLcaCJU In the absence of Rad51, newly replicated strands are extensively resected at dysfunctional replication forks thus generating mitotic sister chromatid bridging ------- COMMENT: 92141eb3ba63c234 4 J/3X7QempEnyRrhjHqrB/PHL7EI (comment: in response to a single blocked replisome) ------- COMMENT: 92141eb3ba63c234 5 J/3X7QempEnyRrhjHqrB/PHL7EI (comment: in response to a single blocked replisome) ------- COMMENT: 92141eb3ba63c234 10 J/3X7QempEnyRrhjHqrB/PHL7EI (comment: in response to a single blocked replisome) ------- COMMENT: 92141eb3ba63c234 11 J/3X7QempEnyRrhjHqrB/PHL7EI (comment: in response to a single blocked replisome) ------- COMMENT: 92141eb3ba63c234 12 J/3X7QempEnyRrhjHqrB/PHL7EI (comment: in response to a single blocked replisome) ------- COMMENT: 92141eb3ba63c234 15 J/3X7QempEnyRrhjHqrB/PHL7EI (comment: in response to a single blocked replisome) ------- COMMENT: 9248f079e7e06dd5 1 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 9248f079e7e06dd5 2 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 9248f079e7e06dd5 3 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 9248f079e7e06dd5 4 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 9248f079e7e06dd5 5 CvUw/JS9dgxB4thoZipZtChOkbY Fig. 3D and E ------- COMMENT: 9248f079e7e06dd5 6 tOmOPs9JendY5r39VoSXnsV78ik Fig. 3F ------- COMMENT: 9248f079e7e06dd5 7 tOmOPs9JendY5r39VoSXnsV78ik Fig. 3F ------- COMMENT: 9248f079e7e06dd5 8 MQHdVxWEBXLXSvso6B2z8ZaGNUE Fig. 3G ------- COMMENT: 9248f079e7e06dd5 9 MQHdVxWEBXLXSvso6B2z8ZaGNUE Fig. 3G ------- COMMENT: 9248f079e7e06dd5 10 4JQt0wN2TH7w8wc2RC2zx0BwanM Fig. S1A ------- COMMENT: 9248f079e7e06dd5 11 7/mZzx8fsG3c4+5psXJodgvVfaQ Fig. 4A and B ------- COMMENT: 9248f079e7e06dd5 12 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: 9248f079e7e06dd5 13 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 9248f079e7e06dd5 14 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 9248f079e7e06dd5 15 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 9248f079e7e06dd5 16 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 9248f079e7e06dd5 17 9Xb+uz4ftlqagKGp7TY1gJc7WdA Fig. 4F ------- COMMENT: 9248f079e7e06dd5 18 9Xb+uz4ftlqagKGp7TY1gJc7WdA Fig. 4F ------- COMMENT: 9248f079e7e06dd5 19 LR03zDKtXw9LS9E+z8jX9MTPg+E Fig. 5A and B ------- COMMENT: 9248f079e7e06dd5 20 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 9248f079e7e06dd5 21 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 9248f079e7e06dd5 22 IgMaceIZ231d1S3E/q+kBcsqFpo Fig. 4H ------- COMMENT: 9248f079e7e06dd5 23 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: 9248f079e7e06dd5 24 mp0M7zDIFggn53e2MCluP++FNgs Fig. 5E ------- COMMENT: 9248f079e7e06dd5 25 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 9248f079e7e06dd5 27 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 9248f079e7e06dd5 28 xLld+U4XtO1Yq5XJ/vA+lPgihjU Fig. 6D ------- COMMENT: 9248f079e7e06dd5 29 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 9248f079e7e06dd5 30 /EKJDn1kiFepVVy1Uoj4agCFYLE Fig. 6E ------- COMMENT: 9248f079e7e06dd5 31 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 9248f079e7e06dd5 32 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 9248f079e7e06dd5 33 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 9248f079e7e06dd5 34 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: 9248f079e7e06dd5 35 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: 9248f079e7e06dd5 36 lKgWJxo2iYvVI77eYhQyWLNq+ZI Fig. 4C, Fig. 4F, Fig. 5A and B, Fig. 5E ------- COMMENT: 92800aaf61c226d9 1 e6fzQMNxxcbMU6MU2EwyJjoDtxc (comment: vw, moved down to -decreased protein targeting to vacuole, with protein secreted) ------- COMMENT: 92800aaf61c226d9 2 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 92800aaf61c226d9 3 TrpGwSfXDB6pmWhIEsjPwKgACUM fig4 (comment: (I moved this down from abnormal endocytisis, is that OK?)) ------- COMMENT: 92800aaf61c226d9 5 CpLPgm8i0a3op3IoshdhYxoBsbU fig 2c ------- COMMENT: 92800aaf61c226d9 6 ssUPqcbJYiCo3qgvOoizS47vzUQ fig 2d ------- COMMENT: 92800aaf61c226d9 7 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 92800aaf61c226d9 8 JncP9WiJ+Fl2byoSVOzu4RAMUAc (comment: CHECK (protein)) ------- COMMENT: 92800aaf61c226d9 11 qW0fooJwQWtfFzpmyf80rEliP/A fig 5c ------- COMMENT: 92a03014a03e35ad 5 Jpl2vaXMJYnwohm38hFbx8PalEg (comment: CHECK swollen) ------- COMMENT: 92a03014a03e35ad 23 Jpl2vaXMJYnwohm38hFbx8PalEg (comment: CHECK swollen) ------- COMMENT: 92a03014a03e35ad 25 z0PUzxcVABiKPOH81fUnXBAhVow (comment: this might be dumbbell ask Jacky) ------- COMMENT: 92bb4e6ba2d974fd 2 2HEdtq538w+ApWoUl0eajizH2BA (comment: low activity) ------- COMMENT: 92bb4e6ba2d974fd 15 mITl9oqD+Gml16+taFNT9Bv62mQ at the second po- sition (Gal-Man-O) ------- COMMENT: 92e8195e77f492c6 11 /7ZjocRSYe/aWBLsirvBrQWQEXs fig2b, c ------- COMMENT: 92e8195e77f492c6 12 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 92e8195e77f492c6 13 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 92e8195e77f492c6 15 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: 92e8195e77f492c6 17 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: 92e8195e77f492c6 18 6FncdJbVGTtZPOl2GRb7uQ+Wx38 fig 1, 3b ------- COMMENT: 92e8195e77f492c6 19 yyMNf1x4ofpAA8TPiE7bhUbV4SU fig 3b ------- COMMENT: 92e8195e77f492c6 20 yyMNf1x4ofpAA8TPiE7bhUbV4SU fig 3b ------- COMMENT: 92e8195e77f492c6 21 yyMNf1x4ofpAA8TPiE7bhUbV4SU fig 3b ------- COMMENT: 92e8195e77f492c6 22 yyMNf1x4ofpAA8TPiE7bhUbV4SU fig 3b ------- COMMENT: 92e8195e77f492c6 23 yyMNf1x4ofpAA8TPiE7bhUbV4SU fig 3b ------- COMMENT: 92e8195e77f492c6 24 y5d5JSva+mfGzPY6bLmr1zIuFbI fig 3e ------- COMMENT: 92e8195e77f492c6 25 y5d5JSva+mfGzPY6bLmr1zIuFbI fig 3e ------- COMMENT: 92e8195e77f492c6 26 y5d5JSva+mfGzPY6bLmr1zIuFbI fig 3e ------- COMMENT: 92e8195e77f492c6 27 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: 92e8195e77f492c6 28 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 92e8195e77f492c6 29 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 92e8195e77f492c6 30 +d8BA6f8pCQnfhdq73s0taZZKpo inferred from combined experiments ------- COMMENT: 92e8195e77f492c6 31 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 92e8195e77f492c6 32 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 92e8195e77f492c6 33 owoQMOffrXW5Zizu2tAmAcvkgAY Table 3 ------- COMMENT: 92e8195e77f492c6 34 owoQMOffrXW5Zizu2tAmAcvkgAY Table 3 ------- COMMENT: 92e8195e77f492c6 35 owoQMOffrXW5Zizu2tAmAcvkgAY Table 3 ------- COMMENT: 92e8195e77f492c6 36 owoQMOffrXW5Zizu2tAmAcvkgAY Table 3 ------- COMMENT: 92e8195e77f492c6 37 owoQMOffrXW5Zizu2tAmAcvkgAY Table 3 ------- COMMENT: 9340627d98142a43 43 v7aVk9xNS+N/el2RIbSwp0P/AZ0 same as crb2delta alone ------- COMMENT: 934af5b83ca408f9 10 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 934af5b83ca408f9 11 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 934af5b83ca408f9 12 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 934af5b83ca408f9 13 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 934af5b83ca408f9 14 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 934af5b83ca408f9 15 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 934af5b83ca408f9 16 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 934af5b83ca408f9 17 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 934af5b83ca408f9 18 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 934af5b83ca408f9 19 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 934af5b83ca408f9 20 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 934af5b83ca408f9 21 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 934af5b83ca408f9 22 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 934af5b83ca408f9 23 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 934af5b83ca408f9 24 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 934af5b83ca408f9 25 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 934af5b83ca408f9 26 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 934af5b83ca408f9 27 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 934af5b83ca408f9 28 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 934af5b83ca408f9 29 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 934af5b83ca408f9 30 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 934af5b83ca408f9 31 Y/rK+VW4RXs7JXT+KWmX2SxPkAs figure 2A ------- COMMENT: 934af5b83ca408f9 32 Y/rK+VW4RXs7JXT+KWmX2SxPkAs figure 2A ------- COMMENT: 934af5b83ca408f9 33 Y/rK+VW4RXs7JXT+KWmX2SxPkAs figure 2A ------- COMMENT: 934af5b83ca408f9 34 Y/rK+VW4RXs7JXT+KWmX2SxPkAs figure 2A ------- COMMENT: 934af5b83ca408f9 35 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 934af5b83ca408f9 36 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 934af5b83ca408f9 37 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 934af5b83ca408f9 38 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 934af5b83ca408f9 39 oJN940NlrCwYqfXpg4AMW5yPjdw figure 3C ------- COMMENT: 934af5b83ca408f9 40 oJN940NlrCwYqfXpg4AMW5yPjdw figure 3C ------- COMMENT: 934af5b83ca408f9 41 oJN940NlrCwYqfXpg4AMW5yPjdw figure 3C ------- COMMENT: 934af5b83ca408f9 42 oJN940NlrCwYqfXpg4AMW5yPjdw figure 3C ------- COMMENT: 934af5b83ca408f9 43 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 934af5b83ca408f9 44 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: 934af5b83ca408f9 45 0fIS+FEv1yhhY+O2Vwe86OJQaZg Figure 4, Table 3 ------- COMMENT: 934af5b83ca408f9 46 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 934af5b83ca408f9 49 owoQMOffrXW5Zizu2tAmAcvkgAY Table 3 ------- COMMENT: 934af5b83ca408f9 50 rPEVwCpvVUMbZFXrjFM4jCtig4w Table 4 ------- COMMENT: 936a8cb7143741d2 3 OBaxwnjXafhawJ9KRK/hZseilw0 The observed pattern was identical, regardless of the presence or not of amino acids in the medium. To confirm the localization to the vacuole membrane, we stained the gtr1-gfp cells with the lipophilic vacuolar membrane fluorescent dye FM4-64. As shown in Fig. 2B, Gtr1–GFP colocalized with FM4-64 staining, indicating that Gtr1–GFP is concentrated at the membranes of vacuoles in S. pombe. ------- COMMENT: 936a8cb7143741d2 5 cPYeNyHKDyljUoJ8k5xXsIzyAcs Fig. 1A Loss of Gtr1 or Gtr2 resulted in the inability of the cells to grow properly, and they divided with a doubling time longer than that of wild-type cells ------- COMMENT: 936a8cb7143741d2 6 cPYeNyHKDyljUoJ8k5xXsIzyAcs Fig. 1A Loss of Gtr1 or Gtr2 resulted in the inability of the cells to grow properly, and they divided with a doubling time longer than that of wild-type cells ------- COMMENT: 936a8cb7143741d2 7 cPYeNyHKDyljUoJ8k5xXsIzyAcs Fig. 1A Loss of Gtr1 or Gtr2 resulted in the inability of the cells to grow properly, and they divided with a doubling time longer than that of wild-type cells ------- COMMENT: 936a8cb7143741d2 8 cPYeNyHKDyljUoJ8k5xXsIzyAcs Fig. 1A Loss of Gtr1 or Gtr2 resulted in the inability of the cells to grow properly, and they divided with a doubling time longer than that of wild-type cells ------- COMMENT: 936a8cb7143741d2 9 cPYeNyHKDyljUoJ8k5xXsIzyAcs Fig. 1A Loss of Gtr1 or Gtr2 resulted in the inability of the cells to grow properly, and they divided with a doubling time longer than that of wild-type cells ------- COMMENT: 936a8cb7143741d2 10 cPYeNyHKDyljUoJ8k5xXsIzyAcs Fig. 1A Loss of Gtr1 or Gtr2 resulted in the inability of the cells to grow properly, and they divided with a doubling time longer than that of wild-type cells ------- COMMENT: 936a8cb7143741d2 11 JrppOcC8yKXkmxKaF7MovZlkJnU Fig. 1B and Fig. 1C, respectively Gtr2, and in particular Gtr1, inhibit sexual differentiation in rich medium. ------- COMMENT: 936a8cb7143741d2 12 JrppOcC8yKXkmxKaF7MovZlkJnU Fig. 1B and Fig. 1C, respectively Gtr2, and in particular Gtr1, inhibit sexual differentiation in rich medium. ------- COMMENT: 936a8cb7143741d2 13 OBaxwnjXafhawJ9KRK/hZseilw0 The observed pattern was identical, regardless of the presence or not of amino acids in the medium. To confirm the localization to the vacuole membrane, we stained the gtr1-gfp cells with the lipophilic vacuolar membrane fluorescent dye FM4-64. As shown in Fig. 2B, Gtr1–GFP colocalized with FM4-64 staining, indicating that Gtr1–GFP is concentrated at the membranes of vacuoles in S. pombe. ------- COMMENT: 936a8cb7143741d2 14 Q7YCUdELEfb4f+tV91if5TOYDyY We observed that Gtr2–RFP co-precipitated with Gtr1 (Fig. 2D) and that the Gtr1–Gtr2 interaction was stronger in cells growing in the presence of amino acids, indicating that the formation of the heterodimer is stimulated by amino acids. ------- COMMENT: 936a8cb7143741d2 16 9jHxqYblM0dQtKA+Ljcq99/O5rk As shown in Fig. 3, GFP–Tor2, GFP–Mip1 and Pop3–GFP showed similar GFP signals that colocalized with FM4-64 staining. Thus, the three components of the TORC1 complex showed vacuolar membrane localization, independently of the presence or not of amino acids in the medium. ------- COMMENT: 936a8cb7143741d2 17 9jHxqYblM0dQtKA+Ljcq99/O5rk As shown in Fig. 3, GFP–Tor2, GFP–Mip1 and Pop3–GFP showed similar GFP signals that colocalized with FM4-64 staining. Thus, the three components of the TORC1 complex showed vacuolar membrane localization, independently of the presence or not of amino acids in the medium. ------- COMMENT: 936a8cb7143741d2 18 9jHxqYblM0dQtKA+Ljcq99/O5rk As shown in Fig. 3, GFP–Tor2, GFP–Mip1 and Pop3–GFP showed similar GFP signals that colocalized with FM4-64 staining. Thus, the three components of the TORC1 complex showed vacuolar membrane localization, independently of the presence or not of amino acids in the medium. ------- COMMENT: 936a8cb7143741d2 19 J0aqpbBvwP2S79icCGoEZimYMvY fig 4a ------- COMMENT: 936a8cb7143741d2 20 J0aqpbBvwP2S79icCGoEZimYMvY fig 4a ------- COMMENT: 936a8cb7143741d2 24 B98ADekPRFSxvZ8vRAUUGPUjUEU the Gtr1–Gtr2 heterodimer and TORC1 are located in the vacuolar membrane independently of the presence of amino acids. However, only when amino acids are present in the medium does the Gtr1–Gtr2 heterodimer interact physically with TORC1 and activate the TOR pathway to induce cell growth and repress sexual differentiation. ------- COMMENT: 936a8cb7143741d2 25 B98ADekPRFSxvZ8vRAUUGPUjUEU the Gtr1–Gtr2 heterodimer and TORC1 are located in the vacuolar membrane independently of the presence of amino acids. However, only when amino acids are present in the medium does the Gtr1–Gtr2 heterodimer interact physically with TORC1 and activate the TOR pathway to induce cell growth and repress sexual differentiation. ------- COMMENT: 936a8cb7143741d2 26 U9Wq7BixA0EDs71Zt6I9pFc0K24 (comment: CHECK ********nitrogen replete/with aa) ------- COMMENT: 936a8cb7143741d2 27 oWkEjhYLw4D2JuwDsi4fpWSznXw The doubling time of vam6D was shorter in the presence of amino acids, indicating that these cells were still able to respond, at least partially, to the presence of amino acids (Fig. 5A) and that Vam6 has an important role in regulating cell growth in S. pombe but is not essential for responding to the availability of amino acids. ------- COMMENT: 936a8cb7143741d2 29 6SRfjVXVeznegnLlcdLxxGLEnqY We introduced Gtr1Q61L in a vam6D background and found that the double mutant was able to grow normally (Fig. 5B), indicating that constitutively active Gtr1 rescues the cell growth defect of the vam6D mutant. ------- COMMENT: 936a8cb7143741d2 30 SbLJYCPLW01aGZKsa2TjAyaJfY8 These results suggest that Vam6 functions upstream of Gtr1, possibly by acting as a GEF. ------- COMMENT: 936a8cb7143741d2 33 m0uMq8OPij/3dRudP5Nju1ZxIVg FM4-64 stained only small vesicles in the cytoplasm of vam6D cells, confirming a defect in vacuolar fusion in these cells. ------- COMMENT: 936a8cb7143741d2 34 m0uMq8OPij/3dRudP5Nju1ZxIVg FM4-64 stained only small vesicles in the cytoplasm of vam6D cells, confirming a defect in vacuolar fusion in these cells. ------- COMMENT: 936a8cb7143741d2 35 m0uMq8OPij/3dRudP5Nju1ZxIVg FM4-64 stained only small vesicles in the cytoplasm of vam6D cells, confirming a defect in vacuolar fusion in these cells. ------- COMMENT: 936a8cb7143741d2 36 htVi9PGBSxtWOqfnvzkm4TQqYI0 (comment: CHECK *****nitrogen replete/with aa*****) vam6D mutant cells showed similar Rps6 phosphorylation levels to that of wild-type cells in the absence of amino acids. However, in contrast to wild- type cells, vam6D cells did not show an increase in Rps6 phosphorylation in the presence of amino acids. ------- COMMENT: 936a8cb7143741d2 37 EnutmpqfIW+nG16aiLgMNJxlFgY (comment: CHECK ***********nitrogen replete/with aa. AND during aa starvation***********)vam6D mutant cells showed similar Rps6 phosphorylation levels to that of wild-type cells in the absence of amino acids. However, in contrast to wild- type cells, vam6D cells did not show an increase in Rps6 phosphorylation in the presence of amino acids. ------- COMMENT: 936a8cb7143741d2 38 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: 936cb01541f616b7 1 vp4kQmZpgu8kiPNKMIU/Bg3sdqw (comment: CHECK in vitro) (Figure 4A, Video 1). ------- COMMENT: 936cb01541f616b7 2 vp4kQmZpgu8kiPNKMIU/Bg3sdqw (comment: CHECK in vitro) (Figure 4A, Video 1). ------- COMMENT: 936cb01541f616b7 3 vp4kQmZpgu8kiPNKMIU/Bg3sdqw (comment: CHECK in vitro) (Figure 4A, Video 1). ------- COMMENT: 936cb01541f616b7 4 vp4kQmZpgu8kiPNKMIU/Bg3sdqw (comment: CHECK in vitro) (Figure 4A, Video 1). ------- COMMENT: 936cb01541f616b7 5 vp4kQmZpgu8kiPNKMIU/Bg3sdqw (comment: CHECK in vitro) (Figure 4A, Video 1). ------- COMMENT: 936cb01541f616b7 6 vp4kQmZpgu8kiPNKMIU/Bg3sdqw (comment: CHECK in vitro) (Figure 4A, Video 1). ------- COMMENT: 938360b9812110c8 40 0HeRLkEe7egImGZc1cl5GYSDJjI actually inferred (IC) from combination of phenotype plus GO:0000014 MF ------- COMMENT: 9390d5b48f1d21af 1 DqBBifI3GI+BjGChioI0oqLo36c (comment: single molecule analysis) ------- COMMENT: 9390d5b48f1d21af 2 DqBBifI3GI+BjGChioI0oqLo36c (comment: single molecule analysis) ------- COMMENT: 9390d5b48f1d21af 3 fePuTyzDqnYQy+RLaoj9pDiVnA8 a stable complex with Sfr1 ------- COMMENT: 9390d5b48f1d21af 4 NihgQ0lL941GeJJihtfCVXAOTUI a stable complex with Swi5 ------- COMMENT: 93c624d8e120755e 1 NOO5bs7UUk7q9ZW6KQ/i/2DSacs Fig1 (comment: Histone H1 used as substrate) ------- COMMENT: 93c624d8e120755e 2 6Nfe5uZWLZqL1x2Njs5sVdUx91k Fig 1 (comment: Histone H1 used as substrate) ------- COMMENT: 93c624d8e120755e 3 IF4IRj5cWh9oIbxC5/0+BIbpcq0 (comment: CHECK [MOVE 'occurs at' TO M-PHASE CYCLIN]) Fig1 and 2B 0.26nM pRum1 inhibits in vitro cdc2-cdc13 kinase activity by ~80%, Fig3C ig5B,C, D addition of 2.6nM rum1 reduces cdc2 associated kinase activity ------- COMMENT: 93c624d8e120755e 4 +BEUXPRilDeUs/UpRi9O5A59chg Fig2C (comment: rum1+ driven by nmt1 promoter in pREP6X is integrated) ------- COMMENT: 93c624d8e120755e 5 oT/OcXiSsH6RWhVLJJEUtUifcUE Fig3A ------- COMMENT: 93c624d8e120755e 6 oT/OcXiSsH6RWhVLJJEUtUifcUE Fig3A ------- COMMENT: 93c624d8e120755e 7 oT/OcXiSsH6RWhVLJJEUtUifcUE Fig3A ------- COMMENT: 93c624d8e120755e 8 oT/OcXiSsH6RWhVLJJEUtUifcUE Fig3A ------- COMMENT: 93c624d8e120755e 9 oT/OcXiSsH6RWhVLJJEUtUifcUE Fig3A ------- COMMENT: 93c624d8e120755e 10 psJbSkNdsDHlhumDf18+Zf6KWvI Fig3B ------- COMMENT: 93c624d8e120755e 11 psJbSkNdsDHlhumDf18+Zf6KWvI Fig3B ------- COMMENT: 93c624d8e120755e 12 GyoNeqKdPv1ukfRUJ8C9QHvcNVI Fig4 (comment: rum1HA pulldown brings down both cdc13 and cdc2 to form rum1-cdc13-cdc2 complex. so I really need to add 3 proteins not two in the annotation extensions) ------- COMMENT: 93c624d8e120755e 13 t6uyTtt4P/8kRlIjC2qelSNbzfs Fig 3C ------- COMMENT: 93c624d8e120755e 14 t6uyTtt4P/8kRlIjC2qelSNbzfs Fig 3C ------- COMMENT: 93c624d8e120755e 15 s8zxygB4j9+cKrsniSSlYhY+9P0 Fig5A ------- COMMENT: 93c624d8e120755e 16 s8zxygB4j9+cKrsniSSlYhY+9P0 Fig5A ------- COMMENT: 93c624d8e120755e 19 JWhAq2HJfej28Yj5AN0YbLX5ck4 FigB over expression reduces cdc2 kinase activity even in absence of added rum1 protein ------- COMMENT: 93c624d8e120755e 20 y/zARJtmjyufy0Qe8ntFqLoX15E Fig5B over expression abolishes cdc13 associated kinase activity even in absence of added rum1 protein ------- COMMENT: 93c624d8e120755e 21 rmUmSxtFO2rGxOtPWW2UNAdzNEA (comment: CHECK [ move to specific cyclin]) Fig6 2.6nM rum1 inhibits cig2 associated cdc2 kinase activity by ~50% ------- COMMENT: 93c624d8e120755e 23 3eCtstf8T3NMz/r3t2XyyDu0+6c Fig 6 cdc2-cig1 complex is insensitive to inhibition by rum1. There is ~100% activity in the presence of 26nM rum ------- COMMENT: 93c99c3ad75ffaf1 14 cnhrL1Ut5IcQVByukqZHkVwss3o (comment: converted from bp by cc) ------- COMMENT: 93c99c3ad75ffaf1 15 cnhrL1Ut5IcQVByukqZHkVwss3o (comment: converted from bp by cc) ------- COMMENT: 93cc304cf9878a34 1 uii7nIsYEYOcLpNZpgfS5nzsuFU Conclusion is dawn by comparing Fig. 1H and Fig. 1I in https://www.micropublication.org/journals/biology/micropub-biology-000508. ------- COMMENT: 93cc304cf9878a34 2 uii7nIsYEYOcLpNZpgfS5nzsuFU Conclusion is dawn by comparing Fig. 1H and Fig. 1I in https://www.micropublication.org/journals/biology/micropub-biology-000508. ------- COMMENT: 93dfcb17ff61262c 15 vpIabTxjcEgArAwgxDayuI8mp4Q (comment: CHECK very mild as shown in xp) ------- COMMENT: 93e1b181a0463890 2 5c4JG5/clsMdXs/QyR9OsxUOGp8 mutant defective in maintenance of quiescence ------- COMMENT: 93e1b181a0463890 3 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 4 KpoRTsEBisav0K7W6KsQsi/tDV8 suppression of G0 maintenance defect ------- COMMENT: 93e1b181a0463890 6 IK3so82hbvRh/RD4PXQK6Q7rW6c Fig S6; (comment: CHECK 15 ug/ml or 20 ug/ml thiabendazole ------- COMMENT: 93e1b181a0463890 7 IK3so82hbvRh/RD4PXQK6Q7rW6c Fig S6; (comment: CHECK 15 ug/ml or 20 ug/ml thiabendazole) ------- COMMENT: 93e1b181a0463890 8 IK3so82hbvRh/RD4PXQK6Q7rW6c Fig S6; (comment: CHECK 15 ug/ml or 20 ug/ml thiabendazole) ------- COMMENT: 93e1b181a0463890 9 IK3so82hbvRh/RD4PXQK6Q7rW6c Fig S6; (comment: CHECK 15 ug/ml or 20 ug/ml thiabendazole) ------- COMMENT: 93e1b181a0463890 11 5c4JG5/clsMdXs/QyR9OsxUOGp8 mutant defective in maintenance of quiescence ------- COMMENT: 93e1b181a0463890 14 5c4JG5/clsMdXs/QyR9OsxUOGp8 mutant defective in maintenance of quiescence ------- COMMENT: 93e1b181a0463890 18 H9U72JPYSJlaGV9AzK09QXlPkKQ 15 ug/ml thiabendazole ------- COMMENT: 93e1b181a0463890 33 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 34 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 35 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 36 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 37 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 38 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 39 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 40 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 43 H9U72JPYSJlaGV9AzK09QXlPkKQ 15 ug/ml thiabendazole ------- COMMENT: 93e1b181a0463890 46 H9U72JPYSJlaGV9AzK09QXlPkKQ 15 ug/ml thiabendazole ------- COMMENT: 93e1b181a0463890 49 H9U72JPYSJlaGV9AzK09QXlPkKQ 15 ug/ml thiabendazole ------- COMMENT: 93e1b181a0463890 50 8TzGX/enMZB38b0U+Cs6hHzE2LQ (comment: defect specific to cycling cells) ------- COMMENT: 93e1b181a0463890 53 H9U72JPYSJlaGV9AzK09QXlPkKQ 15 ug/ml thiabendazole ------- COMMENT: 93e1b181a0463890 56 H9U72JPYSJlaGV9AzK09QXlPkKQ 15 ug/ml thiabendazole ------- COMMENT: 93e1b181a0463890 59 H9U72JPYSJlaGV9AzK09QXlPkKQ 15 ug/ml thiabendazole ------- COMMENT: 93e1b181a0463890 60 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 61 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 62 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 63 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 64 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 65 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 66 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 67 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 68 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 69 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 70 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 71 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 72 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 73 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 74 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 75 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 76 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 77 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 78 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 79 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 80 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 81 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 82 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 83 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 84 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 85 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 86 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 87 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 88 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 89 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 90 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 91 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 92 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 93 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 94 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 95 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 96 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 97 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 98 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 99 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 101 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 102 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 103 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 104 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 105 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 106 gEjWXoV6sUuiwzgInH/wfOIYXjA 24h G0 cell microscopy ------- COMMENT: 93e1b181a0463890 107 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 110 H9U72JPYSJlaGV9AzK09QXlPkKQ 15 ug/ml thiabendazole ------- COMMENT: 93e1b181a0463890 111 H9U72JPYSJlaGV9AzK09QXlPkKQ 15 ug/ml thiabendazole ------- COMMENT: 93e1b181a0463890 112 H9U72JPYSJlaGV9AzK09QXlPkKQ 15 ug/ml thiabendazole ------- COMMENT: 93e1b181a0463890 113 H9U72JPYSJlaGV9AzK09QXlPkKQ 15 ug/ml thiabendazole ------- COMMENT: 93e1b181a0463890 118 MwtweSHdktlmAgd4VaApEqAex/4 suppression by H3K9R mutants ------- COMMENT: 93e1b181a0463890 119 MwtweSHdktlmAgd4VaApEqAex/4 suppression by H3K9R mutants ------- COMMENT: 93e1b181a0463890 120 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 121 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 122 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 124 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 125 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 126 w7hp4dU8O+UiO6WC3ICHGBOWmuU 2 day G0 ChIP ------- COMMENT: 93e1b181a0463890 127 w7hp4dU8O+UiO6WC3ICHGBOWmuU 2 day G0 ChIP ------- COMMENT: 93e1b181a0463890 128 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 129 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 130 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 131 r24usPmvbLxpt3INteBAPGDtOCA S13; Tetrad dissection ------- COMMENT: 93e1b181a0463890 132 r24usPmvbLxpt3INteBAPGDtOCA S13; Tetrad dissection ------- COMMENT: 93e1b181a0463890 133 r24usPmvbLxpt3INteBAPGDtOCA S13; Tetrad dissection ------- COMMENT: 93e1b181a0463890 134 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 135 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 136 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 139 H9U72JPYSJlaGV9AzK09QXlPkKQ 15 ug/ml thiabendazole ------- COMMENT: 93e1b181a0463890 140 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 141 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 142 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 143 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 144 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 145 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 146 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 147 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 148 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 149 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 150 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 151 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 152 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 153 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 154 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 155 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 156 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 157 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 158 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 159 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 160 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 161 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 162 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 163 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 164 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 165 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 166 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 167 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 168 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 169 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 170 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 171 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 175 DLEVulp0yoQ7Hdka9OuDdI0Y5HI Decreased RNA polymerase I at rDNA during vegetative growth; nuc1-FLAG ------- COMMENT: 93e1b181a0463890 176 npZKaeO4AS9M+L7iwxGqrgMV/7k Increase of stalled RNA polymerase I at rDNA during G0 phase; nuc1-FLAG ------- COMMENT: 93e1b181a0463890 177 npZKaeO4AS9M+L7iwxGqrgMV/7k Increase of stalled RNA polymerase I at rDNA during G0 phase; nuc1-FLAG ------- COMMENT: 93e1b181a0463890 178 npZKaeO4AS9M+L7iwxGqrgMV/7k Increase of stalled RNA polymerase I at rDNA during G0 phase; nuc1-FLAG ------- COMMENT: 93e1b181a0463890 179 npZKaeO4AS9M+L7iwxGqrgMV/7k Increase of stalled RNA polymerase I at rDNA during G0 phase; nuc1-FLAG ------- COMMENT: 93e1b181a0463890 183 D7mEDFyl77Rlt8trCDrYlmdTvjQ Increased RNA polymerase II at rDNA during vegetative growth ------- COMMENT: 93e1b181a0463890 184 Jt9bC/xsp3JFjUD6U/0HioUhp84 Increased gH2AX/H2A ratio (marker of DNA damage) at rDNA during G0; 2 day G0 ChIP ------- COMMENT: 93e1b181a0463890 185 Jt9bC/xsp3JFjUD6U/0HioUhp84 Increased gH2AX/H2A ratio (marker of DNA damage) at rDNA during G0; 2 day G0 ChIP ------- COMMENT: 93e1b181a0463890 186 Jt9bC/xsp3JFjUD6U/0HioUhp84 Increased gH2AX/H2A ratio (marker of DNA damage) at rDNA during G0; 2 day G0 ChIP ------- COMMENT: 93e1b181a0463890 187 Jt9bC/xsp3JFjUD6U/0HioUhp84 Increased gH2AX/H2A ratio (marker of DNA damage) at rDNA during G0; 2 day G0 ChIP ------- COMMENT: 93e1b181a0463890 188 jtIEiBDqlPKBKeIkS5bZ2EB5zL8 (comment: CHECK G0-exit) ------- COMMENT: 93e1b181a0463890 189 BB9KrAtpf5+yQqLVrEoymIY4NIs (comment: CHECK Rad22-YFP, G0-exit) ------- COMMENT: 93e1b181a0463890 190 2YgksmC0JY/QjF+Kd4A/VJRxRCE small-RNA-seq ------- COMMENT: 93e1b181a0463890 191 2YgksmC0JY/QjF+Kd4A/VJRxRCE small-RNA-seq ------- COMMENT: 93e1b181a0463890 194 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 195 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 196 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 197 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93e1b181a0463890 198 F6jWrEE4GHmpHxor/Ojz0MnOYK4 G0 viability assay ------- COMMENT: 93eee643544f462e 30 mR98Jr2WH4waSPLdaP7dguUKmX0 (comment: residue not determined experimentally, but probably Y173) ------- COMMENT: 93eee643544f462e 31 mR98Jr2WH4waSPLdaP7dguUKmX0 (comment: residue not determined experimentally, but probably Y173) ------- COMMENT: 93eee643544f462e 33 mR98Jr2WH4waSPLdaP7dguUKmX0 (comment: residue not determined experimentally, but probably Y173) ------- COMMENT: 93eee643544f462e 35 mR98Jr2WH4waSPLdaP7dguUKmX0 (comment: residue not determined experimentally, but probably Y173) ------- COMMENT: 93eee643544f462e 36 mR98Jr2WH4waSPLdaP7dguUKmX0 (comment: residue not determined experimentally, but probably Y173) ------- COMMENT: 93eee643544f462e 38 mR98Jr2WH4waSPLdaP7dguUKmX0 (comment: residue not determined experimentally, but probably Y173) ------- COMMENT: 9431349797d152cf 3 Jesx1GY9jRvA3FCJ0mg01G4d168 does not undergo meiosis under conditions where pat1-114 single mutant does ------- COMMENT: 9431349797d152cf 10 XJkIl+fww2Uv6+Md0AtFc5cAooU (comment: not really sure freq is normal, because wt not shown, but text suggests it's close) ------- COMMENT: 9431349797d152cf 21 NWfvgsnI6RJ8sGe89o5ZWG3/G34 Rad24 sequesters phosphorylated Mei2, preventing Mei2 binding to meiRNA (sme2) ------- COMMENT: 9442751303a5f327 1 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 9442751303a5f327 2 vypE/9RkJ/g9EJO+RWnvoFzccKo fig 3a ------- COMMENT: 9442751303a5f327 3 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: 9442751303a5f327 4 OlMwUr61fG5qlouRE6pMve9rUDo partial rescue of chk1, fig 2b ------- COMMENT: 9442751303a5f327 6 cobSazNkG8nDwK9d1S+b/MM6tJ8 fig 2c ------- COMMENT: 9442751303a5f327 12 ecMu6fH+ipRoDd3Fgf//dOw59tY fig 2a (comment: DROPS TO ZERO) ------- COMMENT: 9442751303a5f327 14 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: 9442751303a5f327 15 b/eo5S//hg5MxZwy8eSQi6WfHVM fig 3 (comment: CHECK fypo/issues/2818) ------- COMMENT: 9442751303a5f327 16 b/eo5S//hg5MxZwy8eSQi6WfHVM fig 3 (comment: CHECK fypo/issues/2818) ------- COMMENT: 9442751303a5f327 17 cobSazNkG8nDwK9d1S+b/MM6tJ8 fig 2c ------- COMMENT: 9442751303a5f327 18 cobSazNkG8nDwK9d1S+b/MM6tJ8 fig 2c ------- COMMENT: 9442751303a5f327 19 FYdmGW4vUjRf/f0A6/tYxYyFPMU fig 3 cells fail to separate and are clupmed together, multiple rounds of nuclear division ------- COMMENT: 9442751303a5f327 20 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 9442751303a5f327 21 cobSazNkG8nDwK9d1S+b/MM6tJ8 fig 2c ------- COMMENT: 947219ec962a1b36 13 +ELajcoaXR08gMX/MFl943K+KSg (comment: CHECK inhibited_by(CHEBI:48828)) ------- COMMENT: 94b54d8b1a931fb9 8 0HRunq8Gfr20S8vY4qdlMMPy8S4 (comment: same as rad51delta alone) ------- COMMENT: 94b54d8b1a931fb9 9 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment: same as either single mutant) ------- COMMENT: 94b54d8b1a931fb9 10 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment: same as either single mutant) ------- COMMENT: 94b54d8b1a931fb9 11 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment: same as either single mutant) ------- COMMENT: 94b54d8b1a931fb9 16 C1XbpcDqRyIpHPhxzvAuiN2V7FI (comment: same as nbs1delta alone) ------- COMMENT: 94b54d8b1a931fb9 17 C1XbpcDqRyIpHPhxzvAuiN2V7FI (comment: same as nbs1delta alone) ------- COMMENT: 94b54d8b1a931fb9 18 C1XbpcDqRyIpHPhxzvAuiN2V7FI (comment: same as nbs1delta alone) ------- COMMENT: 94b54d8b1a931fb9 19 C1XbpcDqRyIpHPhxzvAuiN2V7FI (comment: same as nbs1delta alone) ------- COMMENT: 94d96dad24c762d7 1 otU+boGzgwWc+xfgr+LAF3tLpH8 Figure 2A, Figure 2, D and E ------- COMMENT: 94d96dad24c762d7 2 qp9o6DNrI+TQ7vZs24V94B1bHdU Figure 2A, Figure 2, D and E ------- COMMENT: 94d96dad24c762d7 3 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 94d96dad24c762d7 6 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 94d96dad24c762d7 7 oS07Xg5+tf8cmcJheZqphmFcrp4 Figure 2A Figure 2B and C) ------- COMMENT: 94d96dad24c762d7 8 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 94d96dad24c762d7 9 A6VaqfgQ/A1k15NPioqrDtqiFpM Figure 1H, Table 1 ------- COMMENT: 94d96dad24c762d7 10 OVBD7j1mDXMOhPbh3tHltwZzM6c Figure 1I ------- COMMENT: 94d96dad24c762d7 11 UAXUMVJvPzB/+iCS6kCGmAL1BJs Figure 1J Based on the lower recovery of coimmunoprecipitated proteins, this Spn1p-Spn4p complex appears to be less stable than in the presence of Spn2p or Spn3p. ------- COMMENT: 94d96dad24c762d7 12 vMX1IqxJhT6vQyAnHCShVzl44uo Figure 2, D and E ------- COMMENT: 94d96dad24c762d7 13 xl/GL4rebs2ofn9Ex4dR8GykNjE Figure 5C ------- COMMENT: 94d96dad24c762d7 14 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 94d96dad24c762d7 15 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 94d96dad24c762d7 16 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 94d96dad24c762d7 17 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 94d96dad24c762d7 18 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 94d96dad24c762d7 19 8vBL4cBB7CRJqUvvcjyZsUz9G1A Figure 6A, D, and E ------- COMMENT: 94d96dad24c762d7 20 8vBL4cBB7CRJqUvvcjyZsUz9G1A Figure 6A, D, and E ------- COMMENT: 94d96dad24c762d7 21 8vBL4cBB7CRJqUvvcjyZsUz9G1A Figure 6A, D, and E ------- COMMENT: 94d96dad24c762d7 22 8vBL4cBB7CRJqUvvcjyZsUz9G1A Figure 6A, D, and E ------- COMMENT: 94d96dad24c762d7 23 8vBL4cBB7CRJqUvvcjyZsUz9G1A Figure 6A, D, and E ------- COMMENT: 94d96dad24c762d7 24 8vBL4cBB7CRJqUvvcjyZsUz9G1A Figure 6A, D, and E ------- COMMENT: 94d96dad24c762d7 25 8vBL4cBB7CRJqUvvcjyZsUz9G1A Figure 6A, D, and E ------- COMMENT: 94d96dad24c762d7 26 8vBL4cBB7CRJqUvvcjyZsUz9G1A Figure 6A, D, and E ------- COMMENT: 94d96dad24c762d7 27 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 94d96dad24c762d7 28 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 94d96dad24c762d7 29 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: 94d96dad24c762d7 30 EPUWLoScC8rEyNpFGAqUcB2EMcM Combining this observation with our biochemical data, we conclude that a subcomplex of Spn1p-Spn4p is sufficient for formation of ectopic structures and localizing to the medial cortex, but at least one other septin is required for assembly of a ring structure. ------- COMMENT: 94d96dad24c762d7 31 EPUWLoScC8rEyNpFGAqUcB2EMcM Combining this observation with our biochemical data, we conclude that a subcomplex of Spn1p-Spn4p is sufficient for formation of ectopic structures and localizing to the medial cortex, but at least one other septin is required for assembly of a ring structure. ------- COMMENT: 94d96dad24c762d7 32 UtGYhNEpEg8FAAwb+UPncwc3nJA In this case, Spn3p-GFP was recruited to the medial region before mitosis as before (Figure 8A) and it was now assembled into highly organized ring structures that were easily visualized as split rings once septa had formed (Fig- ure 8, B and C). Virtually no diffuse disk structures were observed (Figure 8, B–D). We conclude from this experiment that Mid2p is solely responsible directly or indirectly for regulating septin ring coalesence in S. pombe. ------- COMMENT: 94d96dad24c762d7 33 EIQ8ZwohAaD1qeRsZvn/9OxJEaA erexpression was the relative persistence of septin rings and the inhibition of mitotic progression, as determined by monitoring the for- mation of binucleates (Figure 8A). This result is consistent with our previous results, indicating that prolonged expres- sion of Mid2p stabilizes septin ring structures and influences cell cycle progression (Tasto et al., 2003). erexpression was the relative persistence of septin rings and the inhibition of mitotic progression, as determined by monitoring the for- mation of binucleates (Figure 8A). This result is consistent with our previous results, indicating that prolonged expres- sion of Mid2p stabilizes septin ring structures and influences cell cycle progression (Tasto et al., 2003). ------- COMMENT: 94e20d207ffa7a12 30 zqIXmvykuzxQkuxzi/YLuntUALM (comment: they don't say whether the OEP populations continue to grow like normal (viable/inviable).) Also data not shown. ------- COMMENT: 94edce7baeb65d9d 4 PxJEJpLb/bwnDIgckm9f2mJQEZs (comment: CHECK The SO ID's correspond to tRNA lys/gln/glu) ------- COMMENT: 94f70c9135257d87 1 qCDacVLrDdH3ZhtMiHO1KMUFw+E figure 1B. ------- COMMENT: 94f70c9135257d87 3 qCDacVLrDdH3ZhtMiHO1KMUFw+E figure 1B. ------- COMMENT: 94f70c9135257d87 4 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: 94f70c9135257d87 5 M1v7Pkv5JpvhTYtTL2+KZqAmhjQ Figure 2A. ------- COMMENT: 94f70c9135257d87 6 M1v7Pkv5JpvhTYtTL2+KZqAmhjQ Figure 2A. ------- COMMENT: 94f70c9135257d87 7 vm11+z0P2PKfJPENV+hMU0ztBW0 Figure 2B. ------- COMMENT: 94f70c9135257d87 8 vm11+z0P2PKfJPENV+hMU0ztBW0 Figure 2B. ------- COMMENT: 94f70c9135257d87 9 vm11+z0P2PKfJPENV+hMU0ztBW0 Figure 2B. ------- COMMENT: 94f70c9135257d87 10 weE0zZ/3cvvqsnBpKD+y6yRP8rM Figure 2B. The sensitivities of Dis1N3A and Dis1C3A were similar to that of the wild-type. ------- COMMENT: 94f70c9135257d87 11 weE0zZ/3cvvqsnBpKD+y6yRP8rM Figure 2B. The sensitivities of Dis1N3A and Dis1C3A were similar to that of the wild-type. ------- COMMENT: 94f70c9135257d87 12 Ow2KdMmahenMAtb60IrQ6XZWgPs Figure 2C. The loss rate of CN2 minichromosome in Dis16A was much higher than that of the wild-type integrant... ------- COMMENT: 94f70c9135257d87 13 6C4gde8IYMXSErYGDtWOAxItCh0 Figure 2C. .... whereas Dis1N3A and Dis1C3A had loss rates that were comparable to those of the wild- type Dis1 integrant. ------- COMMENT: 94f70c9135257d87 14 6C4gde8IYMXSErYGDtWOAxItCh0 Figure 2C. .... whereas Dis1N3A and Dis1C3A had loss rates that were comparable to those of the wild- type Dis1 integrant. ------- COMMENT: 94f70c9135257d87 15 T5GbjnrNxm8yMuXDM324b2354dQ Figure 2C. The loss rate in Dis16E was lower than Dis16A and slightly higher than that in the wild-type. (The Dis16E mutant appears to mimic at least partially the Cdc2-phosphory- lated form of Dis1) ------- COMMENT: 94f70c9135257d87 16 D7UUuQUlIPRI82SRzEU8aM4hPYY These results established that phosphorylation of Dis1 by Cdc2 is required for the high-fidelity segregation of a minichromosome. (comment: (A little bit of curator licence here)) ------- COMMENT: 94f70c9135257d87 17 eL3P0kDkOODN/YwDtKNV+vmemdI Figure 2D. The double mutant mis12 Dis16A failed to produce colonies at 33 ------- COMMENT: 94f70c9135257d87 18 4mEImzaD/C7hcD03r0mYBQNB5NA Figure 2D. whereas mis12 Dis1N3A and mis12 Dis1C3A showed weak inhibition of colony formation ------- COMMENT: 94f70c9135257d87 19 4mEImzaD/C7hcD03r0mYBQNB5NA Figure 2D. whereas mis12 Dis1N3A and mis12 Dis1C3A showed weak inhibition of colony formation ------- COMMENT: 94f70c9135257d87 20 IN9mj9p+1TCK+e3Ri48SRv4JTCM Figure 2D. However, the double mutant mis12 Dis16E could form colonies at 33􏰀C. ------- COMMENT: 94f70c9135257d87 21 CnZ0s0guaE3cdS+2gyih+1a+ON0 (comment: CHECK PHOSPHORYLATED.) Fig3A The Dis1WT-GFP signals are seen as the kinetochore dots in metaphase. AND 4b ------- COMMENT: 94f70c9135257d87 22 EPChj8R/xujJ3uZbg2cu2zCvhaI (comment: CHECK protein localized to spindle (also a child of mislocalized protein)) ------- COMMENT: 94f70c9135257d87 26 5bv1zR6hFUS1Q+pk9Gx5BXmqizE Measurements of the durations of phase 1 (prophase to metaphase), 2 (metaphase to anaphase), and 3 (ana- phase B) on each of the 30 movies of Dis1WT, Dis16A, and Dis16E strains indicated that the timing of mitosis did not seem to be affected by any mutations because mea- sured differences were within the boundaries of experi- mental error (Figure S1C) ------- COMMENT: 94f70c9135257d87 27 5bv1zR6hFUS1Q+pk9Gx5BXmqizE Measurements of the durations of phase 1 (prophase to metaphase), 2 (metaphase to anaphase), and 3 (ana- phase B) on each of the 30 movies of Dis1WT, Dis16A, and Dis16E strains indicated that the timing of mitosis did not seem to be affected by any mutations because mea- sured differences were within the boundaries of experi- mental error (Figure S1C) ------- COMMENT: 94f70c9135257d87 28 Nk6AiKn5R6TYDrkYostev2zi50s Curiously, bent spindles were observed in late anaphase of 53 of 121 Dis16A cells in movies, whereas only 18 of 104 Dis1WT and ten of 127 Dis16E cells examined showed the bent spindle (Figure S1D). ------- COMMENT: 94f70c9135257d87 29 8UW0rfaSLKRoAgAHLnSIL/y1qrM (comment: CHECK UNPHOSPHORYLATED.) In anaphase, Dis1WT-GFP signals abruptly increased along the spindle and at the SPBs despite being absent from the central zone. ------- COMMENT: 94f70c9135257d87 30 qAqchxTQ1iKpFeSXzXmKtwTWzoA Because Dis1WT, Dis16A, and Dis16E all associated with anaphase SPBs, this as- sociation was independent of modification of the mole- cule on the Cdc2 phosphorylation sites. ------- COMMENT: 94f70c9135257d87 32 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: 94f70c9135257d87 33 9eLwe8scFHQDMGaZjVjr4CO1Vs0 In contrast, the Dis16E mutant, which shows the synthetic lethality with Dmtc1, dimin- ished signals along the metaphase spindle. Thus, there is a correlation between the affinity for microtubules of the mutant versions of Dis1 and these mutants’ ability to rescue the Dmtc1 defect. ------- COMMENT: 94fed49a02da5f4a 1 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 94fed49a02da5f4a 2 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 94fed49a02da5f4a 3 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 94fed49a02da5f4a 4 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 94fed49a02da5f4a 6 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 94fed49a02da5f4a 7 PdwhseqlrW5Xuya8UXCRybLS6G0 (comment: DNS) ------- COMMENT: 94fed49a02da5f4a 8 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 94fed49a02da5f4a 9 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 94fed49a02da5f4a 10 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 94fed49a02da5f4a 11 UZGHYRfiPzdTyu+FvIx9wT+AVKw Figure 3B, a–c ------- COMMENT: 94fed49a02da5f4a 12 /ucjDZOEbWBiOOpLw67Qd7/hC7Y Figure 3B, a–c accumulation of presumptive post-Golgi secretory vesicles and abnormal Golgi-like structures were also characteristic of the ypt3-i5 mutants that we reported previously (Cheng et al., 2002). ------- COMMENT: 94fed49a02da5f4a 21 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: 94fed49a02da5f4a 22 HCnes/z8Lqq4SjsoDCUVMcvtTKI When the cells were labeled with FM4-64 for 60 min, the 􏰀apm1 cells were highly frag- mented compared with wild-type cells, consistent with the findings obtained by (Figure 7B) ------- COMMENT: 94fed49a02da5f4a 23 HCnes/z8Lqq4SjsoDCUVMcvtTKI When the cells were labeled with FM4-64 for 60 min, the 􏰀apm1 cells were highly frag- mented compared with wild-type cells, consistent with the findings obtained by (Figure 7B) ------- COMMENT: 94fed49a02da5f4a 25 YtO1nOkgx5dPdCSQSklIJLr3t4g fig8c ------- COMMENT: 94fed49a02da5f4a 27 hQUoVsXaqV8kJGotyEDZ2geMUos (comment: CHECK ypt3-S24N) ------- COMMENT: 95043240cd8d761e 11 mR98Jr2WH4waSPLdaP7dguUKmX0 (comment: residue not determined experimentally, but probably Y173) ------- COMMENT: 95569fb1c0b4c224 1 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: 95569fb1c0b4c224 2 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 95569fb1c0b4c224 3 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: 95569fb1c0b4c224 4 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: 95569fb1c0b4c224 5 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: 95569fb1c0b4c224 6 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: 95569fb1c0b4c224 7 O667UCx0ASHujTPeDrrLDdY4G4w Fig. 1H ------- COMMENT: 95569fb1c0b4c224 8 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 95569fb1c0b4c224 9 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 95569fb1c0b4c224 10 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 95569fb1c0b4c224 11 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 95569fb1c0b4c224 12 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 95569fb1c0b4c224 13 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 95569fb1c0b4c224 14 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 95569fb1c0b4c224 15 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 95569fb1c0b4c224 16 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 95569fb1c0b4c224 17 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 95569fb1c0b4c224 18 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 95569fb1c0b4c224 19 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 95569fb1c0b4c224 20 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 95569fb1c0b4c224 21 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 95569fb1c0b4c224 22 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 95569fb1c0b4c224 23 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 95569fb1c0b4c224 24 hNs98aAZ5GIsSm5wSKzlGrd0S4w Table S1 ------- COMMENT: 95569fb1c0b4c224 25 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 95569fb1c0b4c224 26 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 95569fb1c0b4c224 27 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 95569fb1c0b4c224 28 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 95569fb1c0b4c224 29 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 95569fb1c0b4c224 30 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 95569fb1c0b4c224 31 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 95569fb1c0b4c224 32 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 95569fb1c0b4c224 33 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 95569fb1c0b4c224 34 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: 95569fb1c0b4c224 35 vkT8uMpiBxgGlmYXEA8V3+LXigM Fig. 4E ------- COMMENT: 95569fb1c0b4c224 36 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 95569fb1c0b4c224 37 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 95569fb1c0b4c224 38 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: 957765f909e36351 1 eyr9/uhnCmnEBWeKHfuv0l6Uhlc spExo5 showed activity on either substrate, with a preference for the 5’-ended substrate (Supplementary Fig. S2B). ------- COMMENT: 957765f909e36351 2 nTRXIPUc+7VsOQ5ArGJUgSCVs0A The purified enzyme shows an absorption at 410 nm, characteristic of a [4Fe-4S] iron-sulfur cluster (Fig. 1B,C). ------- COMMENT: 957765f909e36351 3 JGXkzMVEKMUSnJazij4qlQwIDv4 Consistent with these studies, mutation of either of the analogous active site aspartates to alanines (D176A, D207A), abrogated the nuclease activity of spExo5 (Supplementary Fig. S2A). ------- COMMENT: 957765f909e36351 4 JGXkzMVEKMUSnJazij4qlQwIDv4 Consistent with these studies, mutation of either of the analogous active site aspartates to alanines (D176A, D207A), abrogated the nuclease activity of spExo5 (Supplementary Fig. S2A). ------- COMMENT: 957765f909e36351 5 sKKxUBSdRlCvtoxyF1t9Xxh+mQ4 Under these highly inducing conditions, Exo5-FLAG levels were increased dramatically, and cells carrying the Exo5+ plasmid showed a negative growth phenotype (Supplementary Fig. S3B). ------- COMMENT: 957765f909e36351 6 rqj8dQrmH+6TQM88io8oj2xJNZI The affinity-purified wild-type Exo5-FLAG protein showed two prominent species by immunoblot blot analysis (Fig. 2A). The upper band is consistent with the predicted molecular weight of spExo5-3XFLAG protein (~50 kDa), while the lower band is consistent with that of a protein starting at Met58, followed by loss of a small signal peptide upon mitochondrial entry (~43 kDa). Importantly, the M58A mutant lacked the lower band, as one would expect if translation of the mitochondrial species started at Met58 with the M58A mutation eliminating this initiation. Conversely, the Δ(1-57) mutant showed only the lower band, further supporting our model. ------- COMMENT: 957765f909e36351 7 rqj8dQrmH+6TQM88io8oj2xJNZI The affinity-purified wild-type Exo5-FLAG protein showed two prominent species by immunoblot blot analysis (Fig. 2A). The upper band is consistent with the predicted molecular weight of spExo5-3XFLAG protein (~50 kDa), while the lower band is consistent with that of a protein starting at Met58, followed by loss of a small signal peptide upon mitochondrial entry (~43 kDa). Importantly, the M58A mutant lacked the lower band, as one would expect if translation of the mitochondrial species started at Met58 with the M58A mutation eliminating this initiation. Conversely, the Δ(1-57) mutant showed only the lower band, further supporting our model. ------- COMMENT: 957765f909e36351 8 yusPz4kvNZWVhkZbYfhV8UzwD6o Wild-type Exo5+ showed diffuse cytoplasmic fluorescence and both nuclear and punctate mitochondrial fluorescence. The Exo5-M58A mutant showed diffuse cytoplasmic/nuclear fluorescence, but lacked punctate fluorescence suggesting its exclusion from the mitochondria. The Δ(1-57) mutant showed only punctate staining suggesting that this truncated form of Exo5 is solely localized to the mitochondria (Supplementary Table 2). Therefore, both sets of data are consistent with a model in which mitochondrial localization of spExo5 proceeds through translational initiation at Met58, whereas initiation at Met1 yields predominantly the cytoplasmic and nuclear forms. ------- COMMENT: 957765f909e36351 9 yusPz4kvNZWVhkZbYfhV8UzwD6o Wild-type Exo5+ showed diffuse cytoplasmic fluorescence and both nuclear and punctate mitochondrial fluorescence. The Exo5-M58A mutant showed diffuse cytoplasmic/nuclear fluorescence, but lacked punctate fluorescence suggesting its exclusion from the mitochondria. The Δ(1-57) mutant showed only punctate staining suggesting that this truncated form of Exo5 is solely localized to the mitochondria (Supplementary Table 2). Therefore, both sets of data are consistent with a model in which mitochondrial localization of spExo5 proceeds through translational initiation at Met58, whereas initiation at Met1 yields predominantly the cytoplasmic and nuclear forms. ------- COMMENT: 957765f909e36351 10 QEEyIWDcsC0xMc2Als9WHXW0lxg Mass finger printing identified several DNA replication and repair proteins with high significance. The full list of hits with high Mascot scores is in Supplementary Table 3. ------- COMMENT: 957765f909e36351 11 QEEyIWDcsC0xMc2Als9WHXW0lxg Mass finger printing identified several DNA replication and repair proteins with high significance. The full list of hits with high Mascot scores is in Supplementary Table 3. ------- COMMENT: 957765f909e36351 12 QEEyIWDcsC0xMc2Als9WHXW0lxg Mass finger printing identified several DNA replication and repair proteins with high significance. The full list of hits with high Mascot scores is in Supplementary Table 3. ------- COMMENT: 957765f909e36351 13 QEEyIWDcsC0xMc2Als9WHXW0lxg Mass finger printing identified several DNA replication and repair proteins with high significance. The full list of hits with high Mascot scores is in Supplementary Table 3. ------- COMMENT: 957765f909e36351 14 QEEyIWDcsC0xMc2Als9WHXW0lxg Mass finger printing identified several DNA replication and repair proteins with high significance. The full list of hits with high Mascot scores is in Supplementary Table 3. ------- COMMENT: 957765f909e36351 15 QEEyIWDcsC0xMc2Als9WHXW0lxg Mass finger printing identified several DNA replication and repair proteins with high significance. The full list of hits with high Mascot scores is in Supplementary Table 3. ------- COMMENT: 957765f909e36351 16 QEEyIWDcsC0xMc2Als9WHXW0lxg Mass finger printing identified several DNA replication and repair proteins with high significance. The full list of hits with high Mascot scores is in Supplementary Table 3. ------- COMMENT: 957765f909e36351 17 QEEyIWDcsC0xMc2Als9WHXW0lxg Mass finger printing identified several DNA replication and repair proteins with high significance. The full list of hits with high Mascot scores is in Supplementary Table 3. ------- COMMENT: 957765f909e36351 18 5CAZ2G7gJfWJI8emj/dYdZHsvb8 This lethality was not due to the nuclease activity of the protein, since overexpression of the nuclease-deficient mutant (exo5-D207A) showed similar lethality. ------- COMMENT: 957765f909e36351 19 uwKuphIyG/u2X/SIqa7r+B91q2Y Examination of the cell morphology revealed that the cells were elongated, indicative of checkpoint activation [17] (Fig. 4B ------- COMMENT: 957765f909e36351 20 uwKuphIyG/u2X/SIqa7r+B91q2Y Examination of the cell morphology revealed that the cells were elongated, indicative of checkpoint activation [17] (Fig. 4B ------- COMMENT: 957765f909e36351 21 TMbN5Kc+CR8UKGvvQiCNGJt2zlw However, while overexpression of exo5-D207A in a rad3Δ background eliminated the cell elongation phenotype, it did not suppress lethality (Fig. 4A,B) ------- COMMENT: 957765f909e36351 22 TMbN5Kc+CR8UKGvvQiCNGJt2zlw However, while overexpression of exo5-D207A in a rad3Δ background eliminated the cell elongation phenotype, it did not suppress lethality (Fig. 4A,B) ------- COMMENT: 957765f909e36351 23 /gfZtxgEuZIbqvXUv24DcUcRw7A S. pombe exo5Δ strains are viable, indicating that spExo5 is not essential for mitochondrial genome stability (Fig. 2B). ------- COMMENT: 957765f909e36351 24 XVgKdLtipT7pgvD5F0aKq3pexjM Interestingly, while neither the single exo5Δ nor rad2Δ mutant is associated with a detectable mitochondrial growth phenotype, the double mutant exo5Δ rad2Δ showed a failure to grow on media lacking a fermentable carbon source (Fig. 2B). ------- COMMENT: 957765f909e36351 25 508vg/XuUPm8aNp2hS/+pG5u0RE The double mutant also showed a minor growth defect on rich media containing glucose (Fig. 5D) ------- COMMENT: 957765f909e36351 26 ol9sCu6l5bdf4VUco6fMkvE0ko4 We observed a severe loss of mitochondrial DNA from the exo5Δ rad2Δ strain compared to the wild-type and single mutants, suggesting that Exo5 and FEN1 are redundantly required for mitochondrial DNA maintenance (Fig. 2C). ------- COMMENT: 957765f909e36351 27 VL1Kub1Btvu4cdS0s9TqcmkvG8w The results from these experiments establish a redundant function in mitochondria for Exo5 and FEN1, presumably operating during the final steps of DNA replication in order to generate ligatable nicks. ------- COMMENT: 957765f909e36351 28 VL1Kub1Btvu4cdS0s9TqcmkvG8w The results from these experiments establish a redundant function in mitochondria for Exo5 and FEN1, presumably operating during the final steps of DNA replication in order to generate ligatable nicks. ------- COMMENT: 957765f909e36351 29 S82IYoH9QQyZ1CJy/7kSCxjGjfA However, the exo5Δ mutant is more sensitive than isogenic wild-type to UV-irradiation and alkylating agents (Fig. 5A). ------- COMMENT: 957765f909e36351 31 Z/Cg9pgKtKPAWkJsPpdlkgKxFvY Furthermore, the deletion is particularly hypersensitive to interstrand crosslinking (ICL) agents such as 8-methoxypsoralen (Fig. 5B) and cis-platin (Fig. 5C). 8- methoxypsoralen intercalates into the DNA and forms interstrand crosslinks upon irradiation with visible light [23]. ------- COMMENT: 957765f909e36351 35 CwijIbdvBDd0FwP/qBqPY+hUyQQ Fission yeast Exo1 exonuclease is involved in Okazaki fragment maturation, double-strand break repair, mismatch repair, and interstrand crosslink repair [27–29]. While the single exo1Δ and exo5Δ mutants showed a comparable sensitivity to UV, MMS and ICL agents, the double mutant exo1Δ exo5Δ showed an increased sensitivity to these agents, indicating that Exo1 and Exo5 repair these damages with partial redundancy (Fig. 5A, Supplementary Fig. S4E). ------- COMMENT: 957765f909e36351 36 fmUVvrmhh2mILXwm4uYF46+2DiQ The UV sensitivity of a rad13Δ mutant, the 3’-endonuclease that functions in nucleotide excision repair (ortholog of human XPG) is increased in the double mutant with exo5Δ, suggesting that Exo5 does not have a function in nucleotide excision repair. ------- COMMENT: 957765f909e36351 37 Z/Cg9pgKtKPAWkJsPpdlkgKxFvY Furthermore, the deletion is particularly hypersensitive to interstrand crosslinking (ICL) agents such as 8-methoxypsoralen (Fig. 5B) and cis-platin (Fig. 5C). 8- methoxypsoralen intercalates into the DNA and forms interstrand crosslinks upon irradiation with visible light [23]. ------- COMMENT: 957765f909e36351 38 Nu8BLXG/o0NQXa/dgq4bT+JOpag The crosslink sensitivity of pso2Δ is substantially higher than that of exo5Δ (Fig. 5B, C), while the double mutant exo5Δ pso2Δ shows an increased sensitivity to cis-platin (Fig. 5C, Supplementary Fig. S5B ------- COMMENT: 957765f909e36351 39 a+UiTLl2GE3vSVeGt2c7Ho1Kg24 The Fanconi branch of ICL repair is represented by fml1+ and fan1+. Exo5+ is epistatic with fml1+, i.e. the double mutant is not more sensitive than the single mutants (Fig. 5E). Likewise, the exo5Δfan1Δ double mutant is not more sensitive than the single mutants (Supplementary Fig. S5A). These data suggest that Exo5 functions in the Fanconi pathway of ICL repair. ------- COMMENT: 957765f909e36351 40 NnAu6rKHM2BTRksmyr3w/igvs1Y Exo5Δ and pli1Δ show synergistic interactions indicating that they operate in different, competing pathways (Fig. 5F). ------- COMMENT: 957765f909e36351 41 NnAu6rKHM2BTRksmyr3w/igvs1Y Exo5Δ and pli1Δ show synergistic interactions indicating that they operate in different, competing pathways (Fig. 5F). ------- COMMENT: 957765f909e36351 42 eyr9/uhnCmnEBWeKHfuv0l6Uhlc spExo5 showed activity on either substrate, with a preference for the 5’-ended substrate (Supplementary Fig. S2B). ------- COMMENT: 9598b4bb67597469 1 o4bOollYg5nJ/ZdIpQ+huwQsYO0 The capacity of Nth-Spo to generate strand breaks in a variety of damaged plasmid DNA substrates was investigated. Figure 4shows the activity of Nth-Spo and Nth-Eco in incising super- coiled damaged DNA. None of the proteins caused breaks in undamaged DNA. ------- COMMENT: 9598b4bb67597469 2 53mTCcNfFsv/cHRwkxUvgtQhPSU (comment: CHECK thymine glycols, urea) ------- COMMENT: 95b85083b1b32feb 34 umNfifsXZKdwzLVAKvlRE7Gisqg (comment: affects intermolecular, but not intramolecular, end joining) ------- COMMENT: 95b85083b1b32feb 35 umNfifsXZKdwzLVAKvlRE7Gisqg (comment: affects intermolecular, but not intramolecular, end joining) ------- COMMENT: 95b85083b1b32feb 36 umNfifsXZKdwzLVAKvlRE7Gisqg (comment: affects intermolecular, but not intramolecular, end joining) ------- COMMENT: 95b85083b1b32feb 37 umNfifsXZKdwzLVAKvlRE7Gisqg (comment: affects intermolecular, but not intramolecular, end joining) ------- COMMENT: 95b85083b1b32feb 38 umNfifsXZKdwzLVAKvlRE7Gisqg (comment: affects intermolecular, but not intramolecular, end joining) ------- COMMENT: 95b85083b1b32feb 39 umNfifsXZKdwzLVAKvlRE7Gisqg (comment: affects intermolecular, but not intramolecular, end joining) ------- COMMENT: 95b85083b1b32feb 40 umNfifsXZKdwzLVAKvlRE7Gisqg (comment: affects intermolecular, but not intramolecular, end joining) ------- COMMENT: 95c831769f7efdd6 2 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 95c831769f7efdd6 3 7CaA1LG2zBWoTatC9hQXBfh6T5Y fig 2A, Figure 2A ------- COMMENT: 95c831769f7efdd6 4 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: 95c831769f7efdd6 5 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 95c831769f7efdd6 10 y5d5JSva+mfGzPY6bLmr1zIuFbI fig 3e ------- COMMENT: 95c831769f7efdd6 11 YMa3oy4DxKGLS2blIOZW692V4VA fig 3e ------- COMMENT: 95c831769f7efdd6 12 y5d5JSva+mfGzPY6bLmr1zIuFbI fig 3e ------- COMMENT: 95c831769f7efdd6 13 albsh4c4fJ5n6/A0pXFq63upAmc fig 3c ------- COMMENT: 95c831769f7efdd6 14 albsh4c4fJ5n6/A0pXFq63upAmc fig 3c ------- COMMENT: 95c831769f7efdd6 15 23A7gKYH++mwxpDaLPxFOcAYx6Y fig 3c ------- COMMENT: 95c831769f7efdd6 16 FJrUrY/cNbL7O7xtjP6w5yuBdIM fig 2b ------- COMMENT: 95c831769f7efdd6 21 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: 95c831769f7efdd6 32 BLtOmF7J+j17BqYR3EnX/nKOsuc GFP-Rga7(277–695) alone, lacking the majority of the F-BAR domain, could not localize to the PM, resulting in massive cell lysis ------- COMMENT: 95c831769f7efdd6 33 2f2di+eIZFKEvfz1Rum+wKMRCpg (comment: CHECK F-BAR/BAR domain adaptors) Rng10(751–950) interacts directly with the Rga7 F-BAR domain ------- COMMENT: 95c831769f7efdd6 34 2AkAxbcMUJI/Aj1ZFsYukkxEtzc To test for a direct interaction, we performed in vitro binding assays using recombinant Rng10 C terminus and the Rga7 F-BAR (Figures 1D and S2A). GST- Rng10(751–1,038) efficiently bound His6-Rga7(1–320) with a dissociation constant (Kd) of 0.43 μM (Figures 1D, 1E, and S2B). ------- COMMENT: 95c831769f7efdd6 35 gZS0Wt4UMx1Et3BJ6mezRuHqln8 Defining the Rga7-binding motif within Rng10 further, we found that Rng10(751–950) bound Rga7(1–320) with a similar Kd of 0.69 μM (Figures 1F, 1G, and S2B) ------- COMMENT: 95c831769f7efdd6 36 4uEfO+7sibdqWR0GAQArfRpHAHY Fig. 1F ------- COMMENT: 95c831769f7efdd6 37 LDuCn/3qQi1ugZqERWtQz5tyv40 Fig. 2b (comment: CHECK normal lipid binding) ------- COMMENT: 95c831769f7efdd6 38 7CaA1LG2zBWoTatC9hQXBfh6T5Y Figure 2A ------- COMMENT: 95c831769f7efdd6 39 /vWNZMnGjqUzxC2ShnpJejYHDb4 (comment: CHECK MEMBRANE LIPID BINDING) Rga7 F-BAR preferred membranes rich in PI(4)P and PI(4,5)P2 (Figure 3D) ------- COMMENT: 95c831769f7efdd6 40 RYr897pZu5EqG7v4WthlDqXBKGY (comment: CHECK MEMBRANE LIPID BINDING) Rga7 F-BAR preferred membranes rich in PI(4)P and PI(4,5)P2 (Figure 3D) ------- COMMENT: 95ec493fb8fa90cf 1 RzhXXEhfmljM6kUuoeygVxjfskA (comment: CHECK ssu72-C13S) ------- COMMENT: 95ec493fb8fa90cf 7 a5giDoPmZHqaGma5agZ4f+p4Mv8 (comment: CHECK rpb1-T4A and rpb1-S7A) ------- COMMENT: 95ec493fb8fa90cf 9 QaWZUIiVngS/4qTLQyvxxHVifcg (comment: CHECK rpb1-S7A,) Pho1 activity ------- COMMENT: 95ec493fb8fa90cf 10 RQOfo6KX89XwU2z9+6ViicROlSM (comment: CHECK rpb1-T4A,) Pho1 activity ------- COMMENT: 95ec493fb8fa90cf 11 xUWAxdgdPGbVYZKwOzZW32uWpzs Pho1 activity ------- COMMENT: 95ec493fb8fa90cf 12 xUWAxdgdPGbVYZKwOzZW32uWpzs Pho1 activity ------- COMMENT: 95ec493fb8fa90cf 13 ITpyY4SnQmIzAVyWnxEv3YqpIA4 (comment: CHECK ssu72-C13S,) Pho1 activity ------- COMMENT: 95ec493fb8fa90cf 14 xUWAxdgdPGbVYZKwOzZW32uWpzs Pho1 activity ------- COMMENT: 95ec493fb8fa90cf 15 xUWAxdgdPGbVYZKwOzZW32uWpzs Pho1 activity ------- COMMENT: 95ec493fb8fa90cf 17 CtTnGhCjBW/ODzCIB2oZhJQ3/NI (comment: CHECK asp1-H397A) ------- COMMENT: 95ec493fb8fa90cf 19 n2rixW1+NGksXvBzSuKwk5rLT5Q (comment: CHECK asp1-D333A,) Pho1 activity ------- COMMENT: 95ec493fb8fa90cf 20 xUWAxdgdPGbVYZKwOzZW32uWpzs Pho1 activity ------- COMMENT: 95ec493fb8fa90cf 21 riH6PuZeW9pd2w953irT9kPPwpE Figure 8 ------- COMMENT: 95ec493fb8fa90cf 22 riH6PuZeW9pd2w953irT9kPPwpE Figure 8 ------- COMMENT: 95ec493fb8fa90cf 23 riH6PuZeW9pd2w953irT9kPPwpE Figure 8 ------- COMMENT: 95ec493fb8fa90cf 24 riH6PuZeW9pd2w953irT9kPPwpE Figure 8 ------- COMMENT: 95ec493fb8fa90cf 25 riH6PuZeW9pd2w953irT9kPPwpE Figure 8 ------- COMMENT: 95ec493fb8fa90cf 32 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 95ec493fb8fa90cf 33 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 95ec493fb8fa90cf 34 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 95ec493fb8fa90cf 35 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 95ec493fb8fa90cf 36 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 95ec493fb8fa90cf 37 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 95ec493fb8fa90cf 38 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 95ec493fb8fa90cf 39 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 95ec493fb8fa90cf 40 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 95ec493fb8fa90cf 41 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: 95ec493fb8fa90cf 42 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: 95ec493fb8fa90cf 43 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: 95ec493fb8fa90cf 44 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: 95ec493fb8fa90cf 45 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 95ec493fb8fa90cf 46 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: 95ec493fb8fa90cf 47 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 95ec493fb8fa90cf 48 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 95ec493fb8fa90cf 49 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 95ec493fb8fa90cf 50 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 95ec493fb8fa90cf 51 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 95ec493fb8fa90cf 52 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 95ec493fb8fa90cf 53 nHD2Fu3S+9Zw8j4bygLgh1nILhc Northern blotting, Figure 6 ------- COMMENT: 95ec493fb8fa90cf 54 pE2AyeVqk1dZZgE2J8UQU1YFj24 Northern blotting and primer extension, Figure 6 ------- COMMENT: 95ec493fb8fa90cf 55 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 95ec493fb8fa90cf 56 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 95ec493fb8fa90cf 57 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 95ec493fb8fa90cf 58 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 95ec493fb8fa90cf 59 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 95ec493fb8fa90cf 60 YZnuMoJESOcoRynlXoH+KNzXTSM Figure 2C the fivefold de-repression of Pho1 in the aps1Δ strain was enhanced additively to 12- fold in the erh1Δ aps1Δ background ------- COMMENT: 95ec493fb8fa90cf 61 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 95ec493fb8fa90cf 62 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: 95ec493fb8fa90cf 63 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 95ec493fb8fa90cf 64 4mAUCR98QzjQgA+Ly9r98q9nuyU Figure 2 (comment: vw changed from decreased to normal (compared to WT)) ------- COMMENT: 95ec493fb8fa90cf 65 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 95ec493fb8fa90cf 66 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 95ec493fb8fa90cf 67 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 95ec493fb8fa90cf 68 GY9pThaad15PnfYnPJekcdGd8G8 Figure 2 We obtained viable erh1Figure 2A Δ asp1-D333A haploids after mating and sporulation; the double- mutant was slow-growing on YES agar and cold-sensitive: he de-re- pression of Pho1 activity by erh1Δ was erased in the asp1-D333A back- ground ------- COMMENT: 95ec493fb8fa90cf 69 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 95ec493fb8fa90cf 70 ooN6VtLIcekVL7VvwgMA1UAOpbk (comment: (Normal compared to WT)) The instructive findings were that the de-repression of Pho1 by erh1Δ was effaced in rhn1Δ, ssu72-C13S, ctf1Δ, ppn1Δ, and swd22Δ cells and was attenuated in dis2Δ cells (Fig. 4B ------- COMMENT: 95ec493fb8fa90cf 72 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: 95ec493fb8fa90cf 73 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: 95ec493fb8fa90cf 74 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: 95ec493fb8fa90cf 75 6/q1alBKY+SzOo6bNYZuMesD1Rk In addition, using the prt–pho1 reporter plas- mid to gauge Pho1 acid phosphatase expression, we found that Pho1 activity was lower in mmi1Δ cells than in wild-type cells (Fig. 6C) ------- COMMENT: 95ec493fb8fa90cf 76 5DvC1REACbhx6ASNOZKAcBCiyeY suggest ... Erh1 acts as a brake on Mmi1’s ability to promote CPF-de- pendent termination during prt lncRNA synthesis. ------- COMMENT: 95ec493fb8fa90cf 77 10GIoatHUlT04ukieWGVf1cmejg a scenario in which Erh1 acts as a brake on Mmi1’s ability to promote CPF-de- pendent termination during prt lncRNA synthesis. ------- COMMENT: 95faf941a4dcefab 1 eUpn53XkJ7xbUAZHkXQnygsotgI (kcat) was computed as 9.08 s-1. The Michaelis-Menten constant, KM was determined as 0.65 × 101 μM with a maximum velocity (Vmax) of 0.045 μM/s. The substrate specificity ratio, kcat/KM was calculated to be 1.39 × 106 M-1 s-1. ------- COMMENT: 9665521870dab722 2 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 9665521870dab722 3 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 9665521870dab722 4 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: 9665521870dab722 5 uea/SMia5E64tkzXDlnxMYBY2nA (data not shown). ------- COMMENT: 9665521870dab722 6 uea/SMia5E64tkzXDlnxMYBY2nA (data not shown). ------- COMMENT: 9665521870dab722 7 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: 9665521870dab722 8 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 9665521870dab722 9 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 9665521870dab722 10 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: 9665521870dab722 11 BZuCxMac/vSezgqi/zyLm1fasig Figure 4C ------- COMMENT: 9665521870dab722 12 BZuCxMac/vSezgqi/zyLm1fasig Figure 4C ------- COMMENT: 9665521870dab722 13 BZuCxMac/vSezgqi/zyLm1fasig Figure 4C ------- COMMENT: 9665521870dab722 14 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 9665521870dab722 15 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 9665521870dab722 16 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 9665521870dab722 17 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 9665521870dab722 26 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: 9665521870dab722 27 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: 9665521870dab722 28 X9ejb0cfkV1eO4ptUJL9bM2EvDw figure 7A ------- COMMENT: 9665521870dab722 29 vGtIAlSSPNnMrmPTCV9WpCBeLHE (Fig. 7B) ------- COMMENT: 9665521870dab722 30 vGtIAlSSPNnMrmPTCV9WpCBeLHE (Fig. 7B) ------- COMMENT: 9665521870dab722 31 vGtIAlSSPNnMrmPTCV9WpCBeLHE (Fig. 7B) ------- COMMENT: 9665521870dab722 32 vGtIAlSSPNnMrmPTCV9WpCBeLHE (Fig. 7B) ------- COMMENT: 9665521870dab722 33 vGtIAlSSPNnMrmPTCV9WpCBeLHE (Fig. 7B) ------- COMMENT: 9665521870dab722 34 vGtIAlSSPNnMrmPTCV9WpCBeLHE (Fig. 7B) ------- COMMENT: 96964a18b157de92 1 uIg6j8OsyMbew+56IXzqqW6N2M0 Nse4 ubiquitination at K181 and Nse3 at K195 (Data Files S3 and S4). ------- COMMENT: 96964a18b157de92 2 uIg6j8OsyMbew+56IXzqqW6N2M0 Nse4 ubiquitination at K181 and Nse3 at K195 (Data Files S3 and S4). ------- COMMENT: 96964a18b157de92 6 0uHEqzL+Nuq+x8oGbKCon077NZM (Figure 3A) ubiquitin ligase mutant ------- COMMENT: 96964a18b157de92 7 0uHEqzL+Nuq+x8oGbKCon077NZM (Figure 3A) ubiquitin ligase mutant ------- COMMENT: 96964a18b157de92 9 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 96964a18b157de92 10 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: 96964a18b157de92 11 uNHsJ98WbxvxbMcxDeekv0qtlZs (Figure 2D ------- COMMENT: 96964a18b157de92 12 VGfhjqOxHgALIXbuFNmYwKpjF20 Among the Nse1-bound factors, we repeatedly ob- served the Ubc13, Mms2, and Uba1 (Data File S1A,B, Data File S2A–C—ProteomeXchange: PXD029573, and Table S3). ------- COMMENT: 96964a18b157de92 13 esgzthBruLoWWebkR5nqRfX8ViQ Further, using the yeast two-hybrid assay, we discovered that Nse1 interacts with ubiquitin itself, and the interaction site is located in the WHB (aa 117–178) domain of Nse1 (Figures 2E and S4). ------- COMMENT: 96964a18b157de92 14 rTvlsLVKmUoYw6kDPH/eBD/SGFk Nse1- R188E mutant shows synthetic lethality with smc6-74 ------- COMMENT: 96964a18b157de92 16 Z9nJb67mBtObr/fNXzz7qICNu5s severe growth defects with smc6-X and nse6∆ ------- COMMENT: 96964a18b157de92 17 Z9nJb67mBtObr/fNXzz7qICNu5s severe growth defects with smc6-X and nse6∆ ------- COMMENT: 96964a18b157de92 21 DuTYBLfAE/oQYnz94MYxLbLdNhw The Nse1 ubiquitin ligase mutant showed a synthetic relationship with the Nse2 SUMO ligase mutant (C195S, H197A), indicating their separate roles in SMC5/6 function ------- COMMENT: 96964a18b157de92 22 Qvqjljq+NpUmyQ0Fx/OB8ApBdFA Interestingly, the addition of nse1- C216S mutation suppressed the R188E phenotypes (Figure 3A), suggesting that it leads to a ubiquitin-ligase-independent outcome. ------- COMMENT: 96964a18b157de92 23 Qvqjljq+NpUmyQ0Fx/OB8ApBdFA Interestingly, the addition of nse1- C216S mutation suppressed the R188E phenotypes (Figure 3A), suggesting that it leads to a ubiquitin-ligase-independent outcome. ------- COMMENT: 96964a18b157de92 24 3T2pdlLeVaVcrS5X+gJHewu4KYI These synthetic phenotypes were again suppressed by the nse1-C216S mutation (Figure S5). ------- COMMENT: 96964a18b157de92 25 1uFM/w6n8nJGoley/hM8nJAsPyU Figure 1A The MS analysis of the Nse1/3/4- and Ubc13/Mms2-containing in vitro ubiquitination assay led to the identification of Nse4 ubiquitination at K181 and Nse3 at K195 (Data Files S3 and S4). ------- COMMENT: 96964a18b157de92 26 1uFM/w6n8nJGoley/hM8nJAsPyU Figure 1A The MS analysis of the Nse1/3/4- and Ubc13/Mms2-containing in vitro ubiquitination assay led to the identification of Nse4 ubiquitination at K181 and Nse3 at K195 (Data Files S3 and S4). ------- COMMENT: 96d9bd593bfd06c1 1 zEwVxRWoOSkOSRzBQ2W65vqv6UE (comment: CHECK in presence of tschimganine) ------- COMMENT: 96d9bd593bfd06c1 2 zEwVxRWoOSkOSRzBQ2W65vqv6UE (comment: CHECK in presence of tschimganine) ------- COMMENT: 96d9bd593bfd06c1 3 zEwVxRWoOSkOSRzBQ2W65vqv6UE (comment: CHECK in presence of tschimganine) ------- COMMENT: 96d9bd593bfd06c1 4 zEwVxRWoOSkOSRzBQ2W65vqv6UE (comment: CHECK in presence of tschimganine) ------- COMMENT: 96d9bd593bfd06c1 5 zEwVxRWoOSkOSRzBQ2W65vqv6UE (comment: CHECK in presence of tschimganine) ------- COMMENT: 96d9bd593bfd06c1 6 zEwVxRWoOSkOSRzBQ2W65vqv6UE (comment: CHECK in presence of tschimganine) ------- COMMENT: 96d9bd593bfd06c1 7 zEwVxRWoOSkOSRzBQ2W65vqv6UE (comment: CHECK in presence of tschimganine) ------- COMMENT: 96d9bd593bfd06c1 8 zEwVxRWoOSkOSRzBQ2W65vqv6UE (comment: CHECK in presence of tschimganine) ------- COMMENT: 96f359bfc036b3a3 1 /eCx3inf8ahAdVN7dODMYS4LqXo Microscopic examination of hob1D cells revealed them to be slightly but not significantly elongated, relative to an isogenic hob1+ strain (Figure 2b). ------- COMMENT: 96f359bfc036b3a3 2 rrQ8GnF32mBYtv61ZGHaraXWf1A On nitrogen-poor medium or on medium of high osmolarity (i.e. YE/250 mm NaCl), we observed no differences in the growth of hob1+ and hob1D cells (Figure 2c). ------- COMMENT: 96f359bfc036b3a3 3 WnwLchiDK9jFLmycabYQzbxCBKc Thus, unlike RVS167 in budding yeast, hob1+ was dispensable for endocytosis in fission yeast. ------- COMMENT: 96f359bfc036b3a3 4 YjBhD+HXlycA2kBUA6hDNbFwSuY Treat- ment of hob1D cells with the DNA strand-breaking drug phleomycin, a bleomycin analog that is cytotoxic to yeasts (Pramanik et al., 1995), resulted in a marked hypersensitivity in hob1D cells (Figure 4a). In ------- COMMENT: 96f359bfc036b3a3 5 D6yh7IUgRRMEOLNnnWp55aJvE2Q (Figure 4a). In contrast, treatment of hob3D cells did not produce a hypersensi- tive phenotype. ------- COMMENT: 96f359bfc036b3a3 6 2Y1BTGnYTS40AvqbIKEAe2R3ovc UV irradiation resulted in the same phenotypes as those seen in the case of phleomycin: hob1D cells were more sensitive to the effects of UV irradiation than wild-type cells, while hob3D cells were as resistant to UV as were their wild-type cohorts (Figure 4b). ------- COMMENT: 96f359bfc036b3a3 7 2Y1BTGnYTS40AvqbIKEAe2R3ovc UV irradiation resulted in the same phenotypes as those seen in the case of phleomycin: hob1D cells were more sensitive to the effects of UV irradiation than wild-type cells, while hob3D cells were as resistant to UV as were their wild-type cohorts (Figure 4b). ------- COMMENT: 973dc493726740c9 18 WTX0GR/LwEyrdon6Xuudcuu+jq8 requires intact mitotic spindle, as shown by cold-depolymerizing microtubules and nda3 mad2 double mutant phenotype ------- COMMENT: 9754872522d7e5a0 4 a7YmJaWLbI7SUGxV7qq28SlD8Pg (comment: depolymerization (cytoplasmic?)) ------- COMMENT: 9754872522d7e5a0 11 MRTl98rKQY4u69JsQq5X5gUaN4M fig 5C ------- COMMENT: 9754872522d7e5a0 12 zKDIpA3ZI2EIVGn//0dKReACDQ0 Fig. S5B–E ------- COMMENT: 977c41e138dc2e0d 1 5qer2gpHaL28St/Hn65YXSZhChM Cells carrying pREP41Xmid1 exhibited a striking phenotype: they formed bulges near the cell center (Figure 1B). Medial bulges were exhibited in 40% of the cells 20 h after removal of thiamine (Figure 1C middle). Cells were longer than normal, suggestive of a cell cycle delay in interphase (Figure 1B) ------- COMMENT: 977c41e138dc2e0d 2 bAYC4thwAzFAKVtKPLSSndi95Mg and the generation time of the population was increased approximately two-fold (Figure 1C top). ------- COMMENT: 977c41e138dc2e0d 5 RNqxU8k2/bdcE8FAfOkwrHEQNoQ No defects in nuclear positioning were apparent, as nuclei were positioned properly at the middle of the cell or in the bulge region (see Figure 2). Mid1p localization in these ------- COMMENT: 977c41e138dc2e0d 6 mpQ3gtByGy9CPMyGxf+pfftTPoE DNES1-mid1p had a clear defect in nuclear export– in contrast to wild-type mid1p, which exits the nucleus during mitosis, it remained in the nucleus throughout the cell cycle, although some faint rings were occasionally seen (Figure 6, A-B). ------- COMMENT: 977c41e138dc2e0d 7 mpQ3gtByGy9CPMyGxf+pfftTPoE DNES1-mid1p had a clear defect in nuclear export– in contrast to wild-type mid1p, which exits the nucleus during mitosis, it remained in the nucleus throughout the cell cycle, although some faint rings were occasionally seen (Figure 6, A-B). ------- COMMENT: 977c41e138dc2e0d 8 64MIoDabr9Q3VaoymUZHgvthXm0 DNES2-mid1p had a weaker, but demonstrable nuclear export defect: it retained some weak cortical staining and weak rings in addition to nuclear staining (Figure 6C), but ------- COMMENT: 977c41e138dc2e0d 9 64MIoDabr9Q3VaoymUZHgvthXm0 DNES2-mid1p had a weaker, but demonstrable nuclear export defect: it retained some weak cortical staining and weak rings in addition to nuclear staining (Figure 6C), but ------- COMMENT: 977c41e138dc2e0d 10 mpQ3gtByGy9CPMyGxf+pfftTPoE DNES1-mid1p had a clear defect in nuclear export– in contrast to wild-type mid1p, which exits the nucleus during mitosis, it remained in the nucleus throughout the cell cycle, although some faint rings were occasionally seen (Figure 6, A-B). ------- COMMENT: 977c41e138dc2e0d 11 mpQ3gtByGy9CPMyGxf+pfftTPoE DNES1-mid1p had a clear defect in nuclear export– in contrast to wild-type mid1p, which exits the nucleus during mitosis, it remained in the nucleus throughout the cell cycle, although some faint rings were occasionally seen (Figure 6, A-B). ------- COMMENT: 977c41e138dc2e0d 12 ifxpHMHuTJqWt6BV/iUWYNVMZFE inferred from Nuclear export of mid1p was sensitive to leptomycin B (LMB), a drug that blocks the nuclear export factor crm1p (see Figure 8; Nishi et al., 1994; Kudo et al.; 1999), showing that the nuclear export of mid1p is crm1 dependent. ------- COMMENT: 977c41e138dc2e0d 13 SB/Fb/9JzgaIEhgoken5BihkUIA no NLS*-mid1p was detectable in the nucleus when expressed under the control of mid1 promoter ( ------- COMMENT: 977c41e138dc2e0d 14 kapmt86EWQBBxleVwR+DMZRdZvk noNLS*-mid1p was detectable in the nucleus when expressed under the control of mid1 promoter ( ------- COMMENT: 977c41e138dc2e0d 16 RYMyOwY12IIG4VMjpHfPVjfO2mI localized in an identical manner to wild-type mid1p (Figure 9A) and was fully functional (Figure 7 and Table 2). ------- COMMENT: 977c41e138dc2e0d 17 mpQ3gtByGy9CPMyGxf+pfftTPoE DNES1-mid1p had a clear defect in nuclear export– in contrast to wild-type mid1p, which exits the nucleus during mitosis, it remained in the nucleus throughout the cell cycle, although some faint rings were occasionally seen (Figure 6, A-B). ------- COMMENT: 977c41e138dc2e0d 19 Z/tbmYBTV0dw9Lj8/YvRw4mJMpA (comment: CHECK diffuse cytoplsmic throughout the cell cycle) ------- COMMENT: 977c41e138dc2e0d 21 Z/tbmYBTV0dw9Lj8/YvRw4mJMpA (comment: CHECK diffuse cytoplsmic throughout the cell cycle) ------- COMMENT: 97822a9fb07b8414 7 g67bb09Ozj9mU8BpeRAD0uT1fwY Fig. 1a 'delocalized actin' ------- COMMENT: 97822a9fb07b8414 8 G1njoQt5fNvk6qXQsRXUWrn4QOc Fig. 1c ------- COMMENT: 97822a9fb07b8414 11 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 97822a9fb07b8414 15 olmvRps8Xas6jLuIR/DGdCi9y+4 The other was observed as pairs of di- vided cells associated side-by-side (12%), the cell wall of which appeared fragile and often lysed upon division. ------- COMMENT: 97822a9fb07b8414 16 y3HWZIa48QpobZ559pnCl2wIom0 translocation of actin from one end t the other (also fig7) ------- COMMENT: 97822a9fb07b8414 17 0EHdJ6i+rDFIbpK5J4cd7Cuyw3I more than threefold over compared with wild-type cells (344%) ------- COMMENT: 97822a9fb07b8414 19 n3G7hju0gQH1Q5hltA4Uhxa9cnM These results suggested that Mok1 is an stable integral membrane pro- tein, which is consistent with the presence of several trans- membrane domains (Fig. 2 a). ------- COMMENT: 97822a9fb07b8414 20 QqmZ2VpT2ZYYHTme8ncyeGJ8Pr8 combined localization and membrane fraction ------- COMMENT: 97822a9fb07b8414 21 QqmZ2VpT2ZYYHTme8ncyeGJ8Pr8 combined localization and membrane fraction ------- COMMENT: 97822a9fb07b8414 26 pQYbWJWIT7jnP2c4EJOz5zxCCUU mok1-664􏰌pck2 was synthetically lethal at 30􏰊C, a temperature at which either single mutant could grew (Fig. 10b) ------- COMMENT: 97822a9fb07b8414 27 yiAMaY95TPaV9C/0AURl89JoyEg Synthetic lethality was not observed between mok1- 664 and 􏰌pck1, consistent with our previous result showing that pck2􏰇 plays the major role (Toda et al., 1993) ------- COMMENT: 97822a9fb07b8414 28 pQYbWJWIT7jnP2c4EJOz5zxCCUU mok1-664􏰌pck2 was synthetically lethal at 30􏰊C, a temperature at which either single mutant could grew (Fig. 10b) ------- COMMENT: 97822a9fb07b8414 29 56upH219LzmKLOA4rWYgM2tB5PY pck2delta rescues ags1 ox defect, we cant do this genetic interaction type in new interface ------- COMMENT: 9786a292cff06a8a 2 MY9+2pMHcEj6rqpMDSkZva2dHHY (comment: assayed using Tf1 transposon plasmid construct) ------- COMMENT: 9786a292cff06a8a 4 ax6r+zPtZqDRVmgHyqW246ZndUc (comment: assayed using Tf1 transposon) ------- COMMENT: 9786a292cff06a8a 6 RbdncrupoKaqVzf/Y53fgiAbXrY (comment: assayed using Tf1 transposon Gag and IN) ------- COMMENT: 9786a292cff06a8a 8 ax6r+zPtZqDRVmgHyqW246ZndUc (comment: assayed using Tf1 transposon) ------- COMMENT: 9786a292cff06a8a 10 MY9+2pMHcEj6rqpMDSkZva2dHHY (comment: assayed using Tf1 transposon plasmid construct) ------- COMMENT: 97c70bbdfd19df1d 3 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 97c70bbdfd19df1d 4 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 97c70bbdfd19df1d 7 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 97c70bbdfd19df1d 8 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 97c70bbdfd19df1d 9 0UQlRhQ1/a4KCrQD1/b/QvEiY7E Fig. S2, 3 ------- COMMENT: 97c70bbdfd19df1d 10 0UQlRhQ1/a4KCrQD1/b/QvEiY7E Fig. S2, 3 ------- COMMENT: 97c70bbdfd19df1d 11 26IPEgtpLQDbYGhd/m+Y0wNgOZM Fig. 1D ------- COMMENT: 97c70bbdfd19df1d 12 26IPEgtpLQDbYGhd/m+Y0wNgOZM Fig. 1D ------- COMMENT: 97c70bbdfd19df1d 13 26IPEgtpLQDbYGhd/m+Y0wNgOZM Fig. 1D ------- COMMENT: 97c70bbdfd19df1d 14 26IPEgtpLQDbYGhd/m+Y0wNgOZM Fig. 1D ------- COMMENT: 97c70bbdfd19df1d 16 26IPEgtpLQDbYGhd/m+Y0wNgOZM Fig. 1D ------- COMMENT: 97c70bbdfd19df1d 17 26IPEgtpLQDbYGhd/m+Y0wNgOZM Fig. 1D ------- COMMENT: 97c70bbdfd19df1d 20 26IPEgtpLQDbYGhd/m+Y0wNgOZM Fig. 1D ------- COMMENT: 97c70bbdfd19df1d 21 26IPEgtpLQDbYGhd/m+Y0wNgOZM Fig. 1D ------- COMMENT: 97c70bbdfd19df1d 22 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 97c70bbdfd19df1d 23 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 97c70bbdfd19df1d 24 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 97c70bbdfd19df1d 26 wvBb1syfMpxkifxUXOCpt0ZwJoQ (comment: CHECK pcp1-18 rescued by) ------- COMMENT: 97c70bbdfd19df1d 27 wvBb1syfMpxkifxUXOCpt0ZwJoQ (comment: CHECK pcp1-18 rescued by) ------- COMMENT: 97c70bbdfd19df1d 31 88HmluttrLrvFgJFx9FDMacr3OM fig6a ------- COMMENT: 97c70bbdfd19df1d 32 wvBb1syfMpxkifxUXOCpt0ZwJoQ (comment: CHECK pcp1-18 rescued by) ------- COMMENT: 97c70bbdfd19df1d 33 88HmluttrLrvFgJFx9FDMacr3OM fig6a ------- COMMENT: 9835916579ea9c49 16 Wk7vYA8G7ONVLRKrQWbOixaKSZ4 enhanced H3K9me loss at centromere in double mutants ------- COMMENT: 9835916579ea9c49 19 qz+I0kAF2yQMspnmIt22Doh/D68 (comment: CONDITION 18 °C) ------- COMMENT: 9835916579ea9c49 20 qz+I0kAF2yQMspnmIt22Doh/D68 (comment: CONDITION 18 °C) ------- COMMENT: 9835916579ea9c49 21 qz+I0kAF2yQMspnmIt22Doh/D68 (comment: CONDITION 18 °C) ------- COMMENT: 9835916579ea9c49 47 qz+I0kAF2yQMspnmIt22Doh/D68 (comment: CONDITION 18 °C) ------- COMMENT: 9835916579ea9c49 50 qz+I0kAF2yQMspnmIt22Doh/D68 (comment: CONDITION 18 °C) ------- COMMENT: 9835916579ea9c49 51 qz+I0kAF2yQMspnmIt22Doh/D68 (comment: CONDITION 18 °C) ------- COMMENT: 9835916579ea9c49 52 qz+I0kAF2yQMspnmIt22Doh/D68 (comment: CONDITION 18 °C) ------- COMMENT: 9835916579ea9c49 53 qz+I0kAF2yQMspnmIt22Doh/D68 (comment: CONDITION 18 °C) ------- COMMENT: 9835916579ea9c49 54 kXGiWqo2JiHHRRwWVyg3zrCkCyQ (comment: non-canonical termination sites) ------- COMMENT: 9835916579ea9c49 56 qz+I0kAF2yQMspnmIt22Doh/D68 (comment: CONDITION 18 °C) ------- COMMENT: 9835916579ea9c49 59 qz+I0kAF2yQMspnmIt22Doh/D68 (comment: CONDITION 18 °C) ------- COMMENT: 9835916579ea9c49 60 qz+I0kAF2yQMspnmIt22Doh/D68 (comment: CONDITION 18 °C) ------- COMMENT: 9835916579ea9c49 61 qz+I0kAF2yQMspnmIt22Doh/D68 (comment: CONDITION 18 °C) ------- COMMENT: 9835916579ea9c49 62 qz+I0kAF2yQMspnmIt22Doh/D68 (comment: CONDITION 18 °C) ------- COMMENT: 9835916579ea9c49 63 kXGiWqo2JiHHRRwWVyg3zrCkCyQ (comment: non-canonical termination sites) ------- COMMENT: 9835916579ea9c49 65 qz+I0kAF2yQMspnmIt22Doh/D68 (comment: CONDITION 18 °C) ------- COMMENT: 9835916579ea9c49 66 qz+I0kAF2yQMspnmIt22Doh/D68 (comment: CONDITION 18 °C) ------- COMMENT: 9835916579ea9c49 71 kXGiWqo2JiHHRRwWVyg3zrCkCyQ (comment: non-canonical termination sites) ------- COMMENT: 9835916579ea9c49 74 IwnlfZZQbLb6mtkCcmlyOsbC8sc (comment: si independent pericentric heterochromatin formation CPF and RNAi Act in Parallel to Assemble Centromeric Heterochromatin) ------- COMMENT: 9835916579ea9c49 75 IwnlfZZQbLb6mtkCcmlyOsbC8sc (comment: si independent pericentric heterochromatin formation CPF and RNAi Act in Parallel to Assemble Centromeric Heterochromatin) ------- COMMENT: 9846a03793b7b22e 2 EHG4nZ6lZZfhNlkSUuEJu+asA7E (comment: CHECK residue T11) ------- COMMENT: 9846a03793b7b22e 3 EHG4nZ6lZZfhNlkSUuEJu+asA7E (comment: CHECK residue T11) ------- COMMENT: 9846a03793b7b22e 4 EHG4nZ6lZZfhNlkSUuEJu+asA7E (comment: CHECK residue T11) ------- COMMENT: 9846a03793b7b22e 5 EHG4nZ6lZZfhNlkSUuEJu+asA7E (comment: CHECK residue T11) ------- COMMENT: 9846a03793b7b22e 7 EHG4nZ6lZZfhNlkSUuEJu+asA7E (comment: CHECK residue T11) ------- COMMENT: 9846a03793b7b22e 11 EHG4nZ6lZZfhNlkSUuEJu+asA7E (comment: CHECK residue T11) ------- COMMENT: 9846a03793b7b22e 15 EHG4nZ6lZZfhNlkSUuEJu+asA7E (comment: CHECK residue T11) ------- COMMENT: 9846a03793b7b22e 19 EHG4nZ6lZZfhNlkSUuEJu+asA7E (comment: CHECK residue T11) ------- COMMENT: 9846a03793b7b22e 24 EHG4nZ6lZZfhNlkSUuEJu+asA7E (comment: CHECK residue T11) ------- COMMENT: 9846a03793b7b22e 25 EHG4nZ6lZZfhNlkSUuEJu+asA7E (comment: CHECK residue T11) ------- COMMENT: 9846a03793b7b22e 26 EHG4nZ6lZZfhNlkSUuEJu+asA7E (comment: CHECK residue T11) ------- COMMENT: 9846a03793b7b22e 33 QwMGXUFI/jnz6zGL0jZ1+BwMLy4 inferred from phenotypes of mrc1delta, rad3delta, Cds1-Rad26 fusion, other cds1 alleles, and combinations thereof ------- COMMENT: 985b694ee778db28 1 7GqLSHvzmbNE9wTX04txMXtDKJk Tolerance of the 8oxoguanine lesion during DNA gap-filling inserting the ribonucleotide ATP. This acitivity can be coupled to the non-homologous end joining (NHEJ) of double strand breaks (DSBs). (comment: changed from dna repair to translesion synthesis. /AL) ------- COMMENT: 985b694ee778db28 2 3ZT+aLh6RqhDw45W1Ag7ZnvPghI Incision of ribonucleotides paired to 8oxoguanine in the DNA ------- COMMENT: 985b694ee778db28 3 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 985b694ee778db28 7 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: 985b694ee778db28 9 aAcKoQEm9P32YQalyHOis4v+JY4 (comment: RER should probably be a child of this) ------- COMMENT: 9869aba8a5a2370a 1 ifYimhGsTrqlU8Gj6pbXj6dINzU (comment: small) ------- COMMENT: 9869aba8a5a2370a 2 mvWSBrK2wcrQ+JAH6p3oiaDrKpk (comment: bulky) ------- COMMENT: 9869aba8a5a2370a 14 qR5y+jb5N6vxpNoZC69c5VTBNVc (comment: CHECK NMeed to check, its decreased duration of replication arrest?) ------- COMMENT: 987e13c5387ab8d6 11 yCSpItIDxcKeoL55xd28dp/0xaY (comment: CHECK affecting sua1 affecting cys11 affecting met14) ------- COMMENT: 987e13c5387ab8d6 14 E6+razSHhRyDduXZivGAOU7WGnw (comment: CHECK affecting gst2) ------- COMMENT: 987e13c5387ab8d6 15 E6+razSHhRyDduXZivGAOU7WGnw (comment: CHECK affecting gst2) ------- COMMENT: 987e13c5387ab8d6 16 E6+razSHhRyDduXZivGAOU7WGnw (comment: CHECK affecting gst2) ------- COMMENT: 987e13c5387ab8d6 17 EW/zOIU4SfkceVCQLFoGrM/Qu1k Cys2 is a serine O-acetyltransferase required for cysteine biosynthesis Functional profiling revealed that Cys2 is essential for As/Cd tolerance (Table S5). Cys2 has greatest sequence homology to homoserine O-acetyltransferases, predicting that it should be involved with methionine biosynthesis. However, as noted previously, Met6 is also predicted to be a homoserine O-acetyltransferase (Ma et al. 2007). S. cerevisiae homoserine O-acetyltransferase Met2 is significantly more similar to Met6 than Cys2 in S. pombe, suggesting that in S. pombe Met6 is more likely the authentic homoserine O-acetyltransferase (Figure 3). Furthermore, met6D mutants require methionine supplementation for growth on defined minimal medium whereas cys2D cells require cysteine supplementation (Ma et al. 2007). Thus our data showing that cys2D but not met6D cells are highly sensitive to As/Cd toxicity, as well as our data indicating that methionine biosynthesis is not required for cadmium or arsenic resistance, support the notion that Cys2 is actually a serine O-acetyltransferase that is specifically essential for cysteine biosynthesis (Figure 3). ------- COMMENT: 987e13c5387ab8d6 18 EW/zOIU4SfkceVCQLFoGrM/Qu1k Cys2 is a serine O-acetyltransferase required for cysteine biosynthesis Functional profiling revealed that Cys2 is essential for As/Cd tolerance (Table S5). Cys2 has greatest sequence homology to homoserine O-acetyltransferases, predicting that it should be involved with methionine biosynthesis. However, as noted previously, Met6 is also predicted to be a homoserine O-acetyltransferase (Ma et al. 2007). S. cerevisiae homoserine O-acetyltransferase Met2 is significantly more similar to Met6 than Cys2 in S. pombe, suggesting that in S. pombe Met6 is more likely the authentic homoserine O-acetyltransferase (Figure 3). Furthermore, met6D mutants require methionine supplementation for growth on defined minimal medium whereas cys2D cells require cysteine supplementation (Ma et al. 2007). Thus our data showing that cys2D but not met6D cells are highly sensitive to As/Cd toxicity, as well as our data indicating that methionine biosynthesis is not required for cadmium or arsenic resistance, support the notion that Cys2 is actually a serine O-acetyltransferase that is specifically essential for cysteine biosynthesis (Figure 3). ------- COMMENT: 987e13c5387ab8d6 21 yCSpItIDxcKeoL55xd28dp/0xaY (comment: CHECK affecting sua1 affecting cys11 affecting met14) ------- COMMENT: 987e13c5387ab8d6 22 yCSpItIDxcKeoL55xd28dp/0xaY (comment: CHECK affecting sua1 affecting cys11 affecting met14) ------- COMMENT: 987e13c5387ab8d6 23 QmMjZWSPGH4F1C+KFQ9bk/UwK8k A requirement for Mms19 is explained by its role in directing iron-sulfur cluster assembly into sulfite reductase as opposed to promoting DNA repair ------- COMMENT: 98f0242de16564e8 1 Ci+bKhUitsutwHeHgf5TiETS96Y Fig1 ------- COMMENT: 98f0242de16564e8 2 r/WoVd5+uqabzpDBNhmn0l39Tv4 Fig 2B (comment: Histone H1 used as cdc2 substrate Chk2 expressed from nmt1 promoter) ------- COMMENT: 98f0242de16564e8 3 N5iyUwdxEZ4wQQ1PrkqW4eij1Cs Fig 2C ------- COMMENT: 98f0242de16564e8 4 4oQlUUq2rcPaKwNNOwtKM57xzMo Fig 2A ------- COMMENT: 98f0242de16564e8 5 ydaU/PBxwwbn75pTND6x70f4omk Fig 2D ------- COMMENT: 98f0242de16564e8 6 ydaU/PBxwwbn75pTND6x70f4omk Fig 2D ------- COMMENT: 98f0242de16564e8 7 72lJP5My3jst3/bg3iy19wAA0lM Fig 3C cell elongation as a result of chk1 over expression is dependent on wee1+ ------- COMMENT: 98f0242de16564e8 8 LouYBmQo7D4GRE0Z+srWoVnIe5Q Fig 3C wee cell phenotype cell cycle distribution FACS profile of vegetatively growing wee cells show a cell cycle distribution with increased number of cells with a 1C DNA content compared to wild type cells. Note the increase in the G1 peak depends on the size of the cell and semi-wee cells do not always shown an increased G1 peak ------- COMMENT: 98f0242de16564e8 9 YGTHdFPWAmmR96d6Ldv0NPUQzEU Fig 3A ------- COMMENT: 98f0242de16564e8 10 Vg36Pb3XgZZRHTX+vSWCnG6rMUM Fig 3B ------- COMMENT: 98f0242de16564e8 11 Vg36Pb3XgZZRHTX+vSWCnG6rMUM Fig 3B ------- COMMENT: 98f0242de16564e8 12 nf7bG32w+5tFKwTaxzTpAog+x40 Data not shown chk1+ over expression phenotype is suppressed by over expressing cdc25+ independently of cdr1 ------- COMMENT: 98f0242de16564e8 13 L4AiPxBRq4n+PJSzamBTjFepO14 Fig 4 ------- COMMENT: 98f0242de16564e8 14 UtjEPPWnPQLimbycy6uPOnbUP1E Fig 5, Fig6 ------- COMMENT: 98f0242de16564e8 19 29Iedki/OxlajbtXN0DoObPj/+A Fig 6 GST wee1 has been phosphorylated in vitro by chk1. This assay shows that phosphorylation of wee1 by chk1 does not affect wee1 kinase activity ------- COMMENT: 99136d907cd60958 1 qlmYYYdGnX9E3//TAq1AX+zXJBQ (comment: CHECK assayed using casein) ------- COMMENT: 9917fbd53b49c243 28 rq72vfLynyqo5qzyEy/awBLPW08 fig5 (comment: CHECK fusion mutant not currently capturable) ------- COMMENT: 9917fbd53b49c243 35 mgO6I8Bf4LAoyCVfdLYO6a3Si/4 (comment: CHECK Supp) ------- COMMENT: 9917fbd53b49c243 36 r80/zV3Pl+SptjPD03JiMJl0XW0 Fig. S2 ------- COMMENT: 9917fbd53b49c243 37 r80/zV3Pl+SptjPD03JiMJl0XW0 Fig. S2 ------- COMMENT: 9917fbd53b49c243 38 r80/zV3Pl+SptjPD03JiMJl0XW0 Fig. S2 ------- COMMENT: 9917fbd53b49c243 39 r80/zV3Pl+SptjPD03JiMJl0XW0 Fig. S2 ------- COMMENT: 9917fbd53b49c243 41 r80/zV3Pl+SptjPD03JiMJl0XW0 Fig. S2 ------- COMMENT: 992e706ca563bba4 1 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: 992e706ca563bba4 2 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: 992e706ca563bba4 3 ajdfne87YaY/1k5k0Oqi5suprzc Fig. 2C and D ------- COMMENT: 992e706ca563bba4 4 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 992e706ca563bba4 5 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: 992e706ca563bba4 6 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: 992e706ca563bba4 7 Mij9SBEPt4GIt/vtUBP2T7kXS+o Fig. 3B and C ------- COMMENT: 992e706ca563bba4 8 Ryj8ZjtzJ5IswZaD2I1tM2tj06g Tetrad analysis revealed a 2:2 segregation of viability, and all viable progeny were G418 sensitive, indicating that cdk9 + is essential (data not shown). ------- COMMENT: 992e706ca563bba4 9 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 992e706ca563bba4 10 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 992e706ca563bba4 11 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 992e706ca563bba4 12 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 992e706ca563bba4 13 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 992e706ca563bba4 14 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 992e706ca563bba4 15 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 992e706ca563bba4 16 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 992e706ca563bba4 17 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 992e706ca563bba4 18 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 992e706ca563bba4 19 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 992e706ca563bba4 20 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 992e706ca563bba4 21 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 992e706ca563bba4 22 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 992e706ca563bba4 23 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 992e706ca563bba4 24 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 992e706ca563bba4 25 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 992e706ca563bba4 27 D3XvqLa+6AJaJ5MTEGdxcModiTQ Thus, the carboxyl terminus of Cdk9 [...] is required for cell viability. Table 2 ------- COMMENT: 992e706ca563bba4 28 xLld+U4XtO1Yq5XJ/vA+lPgihjU Fig. 6D ------- COMMENT: 992e706ca563bba4 29 xLld+U4XtO1Yq5XJ/vA+lPgihjU Fig. 6D ------- COMMENT: 9937be178c9390b9 11 CwyuurKs7aAxAcxWM53N25ehgmY (comment: CHECK residue=T235 | residue=T187, annotation_extension=added_by(CDK COMPLEX, CDC2 AND CDC13) | residue=T215) ------- COMMENT: 995720e27a54ba1b 34 g3kXLSLDPusFzUiSzIjWT7QL6zQ (comment: CHECK centromere outer repeat transcripts) ------- COMMENT: 995720e27a54ba1b 54 ph5E62fwDm/SkzLoeVAwymA3dac (comment: CONDITION 32 °C) ------- COMMENT: 995720e27a54ba1b 59 ph5E62fwDm/SkzLoeVAwymA3dac (comment: CONDITION 32 °C) ------- COMMENT: 995720e27a54ba1b 60 mHpydKFR1A1+2sN8df1Jx3g3Hac (comment: CONDITION 32 °C;) very slightly worse than without cid12delta ------- COMMENT: 995720e27a54ba1b 61 ph5E62fwDm/SkzLoeVAwymA3dac (comment: CONDITION 32 °C) ------- COMMENT: 995720e27a54ba1b 62 CX5K7ImaQREnUt4bzbqCTu0ZjnA (comment: CONDITION 32 °C;) better than without cid12delta ------- COMMENT: 995720e27a54ba1b 63 ph5E62fwDm/SkzLoeVAwymA3dac (comment: CONDITION 32 °C) ------- COMMENT: 995720e27a54ba1b 64 XYsMWzVfjVeSGjzd2autt/uc/iI (comment: CONDITION 32 °C;) same as without cid12delta ------- COMMENT: 995720e27a54ba1b 65 ph5E62fwDm/SkzLoeVAwymA3dac (comment: CONDITION 32 °C) ------- COMMENT: 995720e27a54ba1b 66 ph5E62fwDm/SkzLoeVAwymA3dac (comment: CONDITION 32 °C) ------- COMMENT: 995720e27a54ba1b 67 cZeQVAD8pPSz05NdjL3BuvSXaAY (comment: CONDITION 26 °C) ------- COMMENT: 995720e27a54ba1b 68 cZeQVAD8pPSz05NdjL3BuvSXaAY (comment: CONDITION 26 °C) ------- COMMENT: 995720e27a54ba1b 69 ph5E62fwDm/SkzLoeVAwymA3dac (comment: CONDITION 32 °C) ------- COMMENT: 99bae54ee5a8b7f0 2 qC5evTwJefgmatLJ/uakkP0Uc/g required for spindle repair following laser ablation ------- COMMENT: 99bae54ee5a8b7f0 3 qC5evTwJefgmatLJ/uakkP0Uc/g required for spindle repair following laser ablation ------- COMMENT: 99bae54ee5a8b7f0 4 6CVIcDt+/y1iDtsq9fRPQTPujPI Not required for spindle repair following laser ablation ------- COMMENT: 99bae54ee5a8b7f0 5 n3ncgl0xoApdWoN7OSiW2Zhsxz4 Microtubule dynamics required for spindle repair following laser ablation ------- COMMENT: 99bc68402289c0b8 1 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 99bc68402289c0b8 2 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: 99bc68402289c0b8 3 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 99bc68402289c0b8 4 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: 99bc68402289c0b8 5 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 99bc68402289c0b8 6 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: 99bc68402289c0b8 7 nzmq7PVenZhB5roCjzaOuFTHs8U If RTS1 function in imprinting and sporulation had been affected because of top1p deficiency, an increase in imprinting and sporulation would be expected. However, no increase was observed, showing that top1p was not involved in termination of replication. ------- COMMENT: 99bc68402289c0b8 8 qnEd6mSTGsCHw0WR7meK1ot8lWM a swi1–111, Dtop1 double mutant revealed a decreased growth rate, suggesting a swi1 and top1 interaction. ------- COMMENT: 99bc68402289c0b8 9 iovJS7KTdBstux9t1k1nxqQYJ14 A similar slow growth phenotype was also observed for a Dtop1, swi3 double mutant (JZ454) when compared to a swi3 mutant strain (E157). ------- COMMENT: 99bc68402289c0b8 10 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 99bc68402289c0b8 11 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 99bc68402289c0b8 12 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 99bc68402289c0b8 13 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 99bc68402289c0b8 15 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 99bc68402289c0b8 16 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: 99bc68402289c0b8 17 HbVMWohg0rhDgD7SIrA9dA+rmAo the swi1p and swi3p proteins, necessary for imprinting, were shown to pause the replication fork at the site of imprinting. ------- COMMENT: 99bc68402289c0b8 18 HbVMWohg0rhDgD7SIrA9dA+rmAo the swi1p and swi3p proteins, necessary for imprinting, were shown to pause the replication fork at the site of imprinting. ------- COMMENT: 99beef0215ee9477 10 L3gOSoBmRfk7HKcZQsxkUk8Wd/4 (comment: CHECK foci disappear in HU; without HU foci appear but with abnormal dynamics) ------- COMMENT: 99beef0215ee9477 11 L3gOSoBmRfk7HKcZQsxkUk8Wd/4 (comment: CHECK foci disappear in HU; without HU foci appear but with abnormal dynamics) ------- COMMENT: 99c47a6d111cc12a 2 GzQQRIirPoWD2NK+XSQSE9jSc3U (comment: CHECK in vitro) ------- COMMENT: 99dde499b1f33c52 4 lzgHTdajFn461pcb7q7tsJ0Qimw The gmn2∆ cells were found to be viable despite growing slightly slower than the wild type (Fig. 3A MM (leu-)) and exhibited the same phenotypes as those of the original gmn2 mutant. ------- COMMENT: 99dde499b1f33c52 5 KtfsniMFylB0SkGlWFLL+LlvZ98 The gmn2∆ cells were highly sen- sitive to hygromycin B, being unable to grow on YES plates containing 25 μg/ml of the drug (Fig. 3A) ------- COMMENT: 99dde499b1f33c52 6 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: 99dde499b1f33c52 7 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: 99dde499b1f33c52 8 40n+UKjQ/dtFnPy7LA1gdrX1V2s As expected, Och1-EGFP expressed in the wild type strain showed strong fluorescence as a typical Golgi-like dots, but faint fluorescent dots were confirmed in gmn2∆ cells (Fig. 4A). ------- COMMENT: 99dde499b1f33c52 9 40n+UKjQ/dtFnPy7LA1gdrX1V2s As expected, Och1-EGFP expressed in the wild type strain showed strong fluorescence as a typical Golgi-like dots, but faint fluorescent dots were confirmed in gmn2∆ cells (Fig. 4A). ------- COMMENT: 99dde499b1f33c52 10 lK3CqQfJELiJc5WjNHATuJYEKjU In contrast, gmn2∆ cells missorted and secreted a significant amount of BiP to the cell surface. These re- sults indicate that Gmn2p is required for normal retention of a luminal ER protein in S. pombe cells. ------- COMMENT: 99dde499b1f33c52 11 Onw8PUUMsPjNJzw4KBr7LvCkq9o (comment: changed from protein retention in ER lumen) ------- COMMENT: 99dde499b1f33c52 12 lK3CqQfJELiJc5WjNHATuJYEKjU In contrast, gmn2∆ cells missorted and secreted a significant amount of BiP to the cell surface. These re- sults indicate that Gmn2p is required for normal retention of a luminal ER protein in S. pombe cells. ------- COMMENT: 99dde499b1f33c52 13 aZ6cVKvy6yvNpbQ1Yxu6hYgGTKI The Gmn2-EGFP protein was recycled back into the ER just as Gms1-EGFP, indicating that Gmn2-EGFP localized mostly to the Golgi membranes (Fig. 6B). ------- COMMENT: 99e9b5b486ebd287 1 kkN5Emmekl8mD04sysZDi+xqaeQ viable spore yield assay ------- COMMENT: 99e9b5b486ebd287 2 kkN5Emmekl8mD04sysZDi+xqaeQ viable spore yield assay ------- COMMENT: 99e9b5b486ebd287 3 kkN5Emmekl8mD04sysZDi+xqaeQ viable spore yield assay ------- COMMENT: 99e9b5b486ebd287 4 ajmnN/OXuKz1gjH2TIbFiBzB1Xc viable spore yield assay; 16% of the surviving spores had inherited two copies of chromosome 3 and were thus aneuploid/diploid. ------- COMMENT: 99e9b5b486ebd287 5 O7x1meg7LPyLAXRmI09NXXeZwek viable spore yield assay; 30% of the surviving spores had inherited the two centromere 3-linked markers suggesting they are aneuploid/diploid. ------- COMMENT: 99e9b5b486ebd287 6 1xAv+w7tKIpMzSRYj50M4RPo2yU viable spore yield assay; 20% of the surviving spores had inherited the two centromere 3-linked markers suggesting they are aneuploid/diploid. ------- COMMENT: 99e9b5b486ebd287 7 kkN5Emmekl8mD04sysZDi+xqaeQ viable spore yield assay ------- COMMENT: 99f58cdf989ca814 4 lSTcsHwGrrlXruLVWUROjYFkeyo This signal was abolished in cell extracts prepared from h2a-SA cells, in which Ser 121 is replaced with alanine (H2A-S121A) in both the hta1+ and hta2+ genes (Fig. 1G). ------- COMMENT: 99f58cdf989ca814 18 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 99f58cdf989ca814 19 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 99f58cdf989ca814 20 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 99f58cdf989ca814 21 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 99f58cdf989ca814 27 MpML2E+8wq5nwC1YfM2LDELzzPU Fig. 2E ------- COMMENT: 99f58cdf989ca814 28 MpML2E+8wq5nwC1YfM2LDELzzPU Fig. 2E ------- COMMENT: 99f58cdf989ca814 29 MpML2E+8wq5nwC1YfM2LDELzzPU Fig. 2E ------- COMMENT: 99f58cdf989ca814 30 MpML2E+8wq5nwC1YfM2LDELzzPU Fig. 2E ------- COMMENT: 99f58cdf989ca814 31 LX30Pzyp0dXIvIlY0jjx8zyNWPA Fig. 2F ------- COMMENT: 99f58cdf989ca814 32 LX30Pzyp0dXIvIlY0jjx8zyNWPA Fig. 2F ------- COMMENT: 99f58cdf989ca814 33 LX30Pzyp0dXIvIlY0jjx8zyNWPA Fig. 2F ------- COMMENT: 99f58cdf989ca814 34 LX30Pzyp0dXIvIlY0jjx8zyNWPA Fig. 2F ------- COMMENT: 99f58cdf989ca814 35 CqEHdyMOJjpHNvsXuKEmvN799p8 (comment: CHECK H2A-SE) fig S4 ------- COMMENT: 99f58cdf989ca814 36 CqEHdyMOJjpHNvsXuKEmvN799p8 (comment: CHECK H2A-SE) fig S4 ------- COMMENT: 99f58cdf989ca814 37 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 99f58cdf989ca814 38 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 99f58cdf989ca814 39 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 99f58cdf989ca814 40 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 99f58cdf989ca814 41 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 99f58cdf989ca814 42 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 99f58cdf989ca814 43 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: 99f58cdf989ca814 44 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 99f58cdf989ca814 45 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 99f58cdf989ca814 46 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 99f58cdf989ca814 47 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 99f58cdf989ca814 48 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 99f58cdf989ca814 49 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: 99f58cdf989ca814 50 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 99f58cdf989ca814 51 mKHrmdoHpr4rPKS2pKRyXvNdG7s Fig. S6 ------- COMMENT: 99f58cdf989ca814 52 mKHrmdoHpr4rPKS2pKRyXvNdG7s Fig. S6 ------- COMMENT: 99f58cdf989ca814 59 mKHrmdoHpr4rPKS2pKRyXvNdG7s Fig. S6 ------- COMMENT: 99f58cdf989ca814 60 mKHrmdoHpr4rPKS2pKRyXvNdG7s Fig. S6 ------- COMMENT: 99f58cdf989ca814 61 mKHrmdoHpr4rPKS2pKRyXvNdG7s Fig. S6 ------- COMMENT: 99f58cdf989ca814 62 mKHrmdoHpr4rPKS2pKRyXvNdG7s Fig. S6 ------- COMMENT: 99f58cdf989ca814 64 mKHrmdoHpr4rPKS2pKRyXvNdG7s Fig. S6 ------- COMMENT: 99f58cdf989ca814 79 glJW1aOyj4jhjS/C9sk9B+zU/dU Fig. 4a ------- COMMENT: 99f58cdf989ca814 80 xK236mXgYloo+mx4w8mXINu2p+I Fig. 4c ------- COMMENT: 99f58cdf989ca814 81 xK236mXgYloo+mx4w8mXINu2p+I Fig. 4c ------- COMMENT: 99f58cdf989ca814 82 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 99f58cdf989ca814 83 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: 99f58cdf989ca814 88 7nkFkejREgDThj8O5WegYsaDowo Fig. 4G ------- COMMENT: 99f58cdf989ca814 89 7nkFkejREgDThj8O5WegYsaDowo Fig. 4G ------- COMMENT: 99f58cdf989ca814 90 7nkFkejREgDThj8O5WegYsaDowo Fig. 4G ------- COMMENT: 99f58cdf989ca814 91 7nkFkejREgDThj8O5WegYsaDowo Fig. 4G ------- COMMENT: 99f58cdf989ca814 92 PboB/GYuErKzkFUYfOYjj0QOgZE fusion experiments (Figure 3) ------- COMMENT: 99f58cdf989ca814 93 PboB/GYuErKzkFUYfOYjj0QOgZE fusion experiments (Figure 3) ------- COMMENT: 99f58cdf989ca814 94 PboB/GYuErKzkFUYfOYjj0QOgZE fusion experiments (Figure 3) ------- COMMENT: 99f58cdf989ca814 95 PboB/GYuErKzkFUYfOYjj0QOgZE fusion experiments (Figure 3) ------- COMMENT: 99f58cdf989ca814 102 d01mbxaRiD1nhaJIcstkO6c29tY (comment: CHECK H2A-S121) phosphorylation was completely abolished in bub1-KD cells, although a similar amount of H2A was detected (Fig. 1G). ------- COMMENT: 9a10046246e64418 58 QzPXsErYMV8Puaxja+1U3s61Pcw (comment: CHECK referred to in PMID:33137119) ------- COMMENT: 9a207bd4727d5b29 20 j4NatnI+vpaWEq11w/rVyU+J/ms single micrograph, so can't tell if they're viable ------- COMMENT: 9a207bd4727d5b29 21 j4NatnI+vpaWEq11w/rVyU+J/ms single micrograph, so can't tell if they're viable ------- COMMENT: 9a3197e7865f3d22 1 ekDPQHbAnbe6qq1nVLc0Qsg/Ow0 Fig1A ------- COMMENT: 9a3197e7865f3d22 2 m+qX6dptcidIfdrWwPm0KrF9Lq0 Fig1D peptide 3 ------- COMMENT: 9a3197e7865f3d22 4 d7sIPFdPZrC35hhhmRqatpq3Vu8 Fig1D peptide 3 and peptide1 ------- COMMENT: 9a3197e7865f3d22 5 d9WnpA++BofNkKyPXc1wm0N54fQ Fig1D peptide 2 ------- COMMENT: 9a3197e7865f3d22 10 yJqhG3hGbolbSidw+G0CSkpZHQA Fig3 chk1 1 is not required for T14 phosphorylation by wee1 ------- COMMENT: 9a3197e7865f3d22 12 VhMUfUk4HLnoom8kSRGVU55KD/Q Table 2 cdc2-T14A is present on multicopy plasmid cells are viable but have a semi wee phenotype ------- COMMENT: 9a3197e7865f3d22 13 6/DwBV4QOg0jmfv/8a8eCHa2Ksw Table 2 cdc2-T14A is present on multicopy plasmid cells are viable and have a normal cell size phenotype ------- COMMENT: 9a3197e7865f3d22 14 8pyXAwWaxj80TUk2EM/uerAOOnA Fig 4A middle panel cells average size 11.6µm This strain is a gene replacement of cdc2+ ------- COMMENT: 9a3197e7865f3d22 15 E79dCaA/kQe6lypFM3Ofkpdwb1w Fig 4A right panel cells average size 16.6µm This strain is a gene replacement of cdc2+ ------- COMMENT: 9a3197e7865f3d22 16 Lji0tNAyLkIJsCpvCJlxQt5QtMA Fig 4B absence of peptide 3 This strain is a gene replacement of cdc2+ ------- COMMENT: 9a3197e7865f3d22 17 vlSlWiM3+axjh3gJVfLCVzi1Lgo Fig 4B middle right panel presence of peptide 3 This strain is a gene replacement of cdc2+ ------- COMMENT: 9a3197e7865f3d22 18 PT9zNvFqJ8D1nnaC89WMkCSm61A Fig 4B absence of peptide 3 Overexpression of wee1 does not phosphorylate T14A residue. This strain is a gene replacement of cdc2+ ------- COMMENT: 9a3197e7865f3d22 19 4+HEKi59CDfOaJOW+gCamNQ2/eo Fig 4B increased peptide 3 compared to when wee1 is not overexpressed . This strain is a gene replacement of cdc2+ ------- COMMENT: 9a3197e7865f3d22 20 xzxLTvPrkKv/e+/tS5QY2nYovDM Fig5B wee1 is necessary for T14 phosphorylation no peptide 3 is observed when wee1 is deleted ------- COMMENT: 9a3197e7865f3d22 22 Vvq4D2jO7B8T1Y4N0voL69J0zzg Fig7A, B At restrictive temperature T14 is not phosphorylated (no peptide when cells blocked at RT.) ------- COMMENT: 9a3197e7865f3d22 23 p34SZqFQN/Ytd0bVK5LMnrsPzSY Fig7A, B At shift to permissive temperature T14 becomes phosphorylated. Peptide 3 is only present at low stoichiometry ------- COMMENT: 9a3197e7865f3d22 24 4R+lBpYtzgzk2vhxC1Wq1WZ0svw Fig8 ------- COMMENT: 9a3197e7865f3d22 25 4R+lBpYtzgzk2vhxC1Wq1WZ0svw Fig8 ------- COMMENT: 9a3197e7865f3d22 26 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: 9a3197e7865f3d22 27 mkG4SVEKKgvGatdN8jne0pKLlPE Fig1B, C and D x = a small phospho peptide of T14Y15. T14 phosphorylation only occurs when wee1 is overexpressed ------- COMMENT: 9a676547ac396223 21 vYqrQBieXhKnCE61pXHc8jTpIXo Fig. S3C ------- COMMENT: 9a676547ac396223 24 hkAYTwXptZJW0cTNkvnkV5Y/U5s Fig. 2d ------- COMMENT: 9a676547ac396223 25 hkAYTwXptZJW0cTNkvnkV5Y/U5s Fig. 2d ------- COMMENT: 9a676547ac396223 26 hkAYTwXptZJW0cTNkvnkV5Y/U5s Fig. 2d ------- COMMENT: 9a676547ac396223 27 pi8tR/OzhaiNy9nAOGhKmN/XxhM Fig. 3c ------- COMMENT: 9a676547ac396223 32 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 9a6cbb66c43cb971 2 FUkFWmlXMrqi9Q39DuXLYTqEFgs microarray data shows 111 genes affected ------- COMMENT: 9a6cbb66c43cb971 10 pQDviJvynclc4ExDDOO7luaLvUs (comment: assayed using ade6-M26) ------- COMMENT: 9a6cbb66c43cb971 28 9zpGGbw2Ekc+gfIBe0SxqCKW5Sc These findings showed that Upf1 is re- quired for degradation of the ade6-M26 mRNA in S. pombe. ------- COMMENT: 9a6cbb66c43cb971 29 9zpGGbw2Ekc+gfIBe0SxqCKW5Sc These findings showed that Upf1 is re- quired for degradation of the ade6-M26 mRNA in S. pombe. ------- COMMENT: 9a6d1a60bd8f2c4f 42 He0wU9A2MHmk5oGjtwBDXW2IApA (comment: CHECK weak) ------- COMMENT: 9a74e80e076097ef 1 dxsPKmPCl1yzyWT5XX6U7TQf/5A (comment: CHECK at division) ------- COMMENT: 9b08e4a8102fdd6a 1 AzPJ3uDvFTdY3knT3/RTd9/xOKw (Fig. 1A-D) ------- COMMENT: 9b08e4a8102fdd6a 2 SvlFFz9CaCJByQRBTLvdFnPqSYg (Fig. 1F) ------- COMMENT: 9b08e4a8102fdd6a 3 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: 9b3c0d00dc3aedb7 29 FRj5MRqYh1ZxdPoR1QPRnT/6f20 (comment: in zygotic nucleus) ------- COMMENT: 9b42d44e8fba951f 4 6xPx2xvncf7TJ9AItOYe0hNCeL8 Our in vitro dephosphor- ylation assay results demonstrated that individually overexpressing PP2A subunits indeed boosted the over- all phosphatase activity compared to expressing endog- enous subunits alone (Figure 3B). ------- COMMENT: 9b42d44e8fba951f 8 FPNU1SIyNlNsDwxx95B3jB8wnqo Pab1 slightly suppressed the SAC silencing defects and allowed dis2Δ cells to enter anaphase earlier after arrest- and-release in the nda3-KM311 mutant background (Figure 2 and Figure S2). ------- COMMENT: 9b42d44e8fba951f 9 GhARQeR9SIfpEe+CWgiWfzpkNeo Overexpression of all Ppa1 slightly suppressed the SAC silencing defects and allowed dis2Δ cells to enter anaphase earlier after arrest-and-release in the nda3-KM311 mutant background. ------- COMMENT: 9b42d44e8fba951f 10 7AhEDlT4TtSI9VVe8qPexjShNSA Overexpression of Ppa2 slightly suppressed the SAC silencing defects and allowed dis2Δ cells to enter anaphase earlier after arrest-and-release in the nda3-KM311 mutant background. ------- COMMENT: 9b42d44e8fba951f 11 7AhEDlT4TtSI9VVe8qPexjShNSA Overexpression of Ppa2 slightly suppressed the SAC silencing defects and allowed dis2Δ cells to enter anaphase earlier after arrest-and-release in the nda3-KM311 mutant background. ------- COMMENT: 9b42d44e8fba951f 12 pORRMRYNlE7SGIhWdXIUVYHiLzk Overexpression of Par1 slightly suppressed the SAC silencing defects and allowed dis2Δ cells to enter anaphase earlier after arrest-and-release in the nda3-KM311 mutant background. ------- COMMENT: 9b42d44e8fba951f 13 A0O6DZYz+iDS33qyuCRZAAL34oI Overexpression of Par2 suppressed the SAC silencing defects and allowed dis2Δ cells to enter anaphase earlier after arrest-and-release in the nda3-KM311 mutant background. ------- COMMENT: 9b42d44e8fba951f 14 jakyqE4bA5KDsNgxh1j6mFICJ8U figure 1D ------- COMMENT: 9b42d44e8fba951f 15 jakyqE4bA5KDsNgxh1j6mFICJ8U figure 1D ------- COMMENT: 9b42d44e8fba951f 16 jakyqE4bA5KDsNgxh1j6mFICJ8U figure 1D ------- COMMENT: 9b42d44e8fba951f 17 jakyqE4bA5KDsNgxh1j6mFICJ8U figure 1D ------- COMMENT: 9b42d44e8fba951f 18 jakyqE4bA5KDsNgxh1j6mFICJ8U figure 1D ------- COMMENT: 9b42d44e8fba951f 19 jakyqE4bA5KDsNgxh1j6mFICJ8U figure 1D ------- COMMENT: 9b42d44e8fba951f 20 jakyqE4bA5KDsNgxh1j6mFICJ8U figure 1D ------- COMMENT: 9b42d44e8fba951f 21 jakyqE4bA5KDsNgxh1j6mFICJ8U figure 1D ------- COMMENT: 9b42d44e8fba951f 22 jakyqE4bA5KDsNgxh1j6mFICJ8U figure 1D ------- COMMENT: 9b4ac98c99b4f86c 5 VK/E07z4ngEJDB2yOcuROADoNeQ KΔ::ade6+ monitored by qRT-PCR ------- COMMENT: 9b4ac98c99b4f86c 30 /Lr1mu0HBtaHGWE6vfYNbnmPHCA Loss of the HMG domain of Lsd2 (but not Lsd1) produces inviable cells (lethal). ------- COMMENT: 9b4ac98c99b4f86c 44 VK/E07z4ngEJDB2yOcuROADoNeQ KΔ::ade6+ monitored by qRT-PCR ------- COMMENT: 9b4ac98c99b4f86c 71 eOxwRrM7o+bL/krf8O4pQ8R1X3Y (comment: CHECK compared to lsd1-ao single mutant) ------- COMMENT: 9b4ac98c99b4f86c 72 wH3PK55V1ckwMJSi4KVyL/IxTKE (comment: CHECK compared to Lsd1-ao single mutant) ------- COMMENT: 9b4ac98c99b4f86c 121 VK/E07z4ngEJDB2yOcuROADoNeQ KΔ::ade6+ monitored by qRT-PCR ------- COMMENT: 9b4ac98c99b4f86c 128 Ns1iTeKT3zUUNS86yokJP27GXxQ (comment: CHECK ago1delta, lsd1-deltaHMG) ------- COMMENT: 9b4ac98c99b4f86c 129 ySNixlT4hHI1S565xJs1o7A6WbU (comment: CHECK ago1delta, lsd2-deltaC) ------- COMMENT: 9b4ac98c99b4f86c 151 ruuevdtdIWilBqbDBbXNxNG8PoA (comment: CHECK ago1delta, lsd2-ao) ------- COMMENT: 9b4ac98c99b4f86c 190 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 9b4ac98c99b4f86c 191 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 9b4ac98c99b4f86c 192 cgiu0qmHSEmD2t0EukCUkMPtO9U At all constitutive heterochromatic domains, lsd C-terminal mutants showed strong cumulative genetic interactions with clr3∆ and sir2∆, indicating that they play overlapping functions in epigenetic silencing. In particular, lsd2-∆C sir2∆ double mutants show the strongest silencing defects at centromeric regions and sub-telomeric regions, while lsd1-∆HMG clr3∆ double mutants have the most robust effect at the mating-type locus (Figure 7D). ------- COMMENT: 9b4ac98c99b4f86c 193 cgiu0qmHSEmD2t0EukCUkMPtO9U At all constitutive heterochromatic domains, lsd C-terminal mutants showed strong cumulative genetic interactions with clr3∆ and sir2∆, indicating that they play overlapping functions in epigenetic silencing. In particular, lsd2-∆C sir2∆ double mutants show the strongest silencing defects at centromeric regions and sub-telomeric regions, while lsd1-∆HMG clr3∆ double mutants have the most robust effect at the mating-type locus (Figure 7D). ------- COMMENT: 9b4ac98c99b4f86c 194 cgiu0qmHSEmD2t0EukCUkMPtO9U At all constitutive heterochromatic domains, lsd C-terminal mutants showed strong cumulative genetic interactions with clr3∆ and sir2∆, indicating that they play overlapping functions in epigenetic silencing. In particular, lsd2-∆C sir2∆ double mutants show the strongest silencing defects at centromeric regions and sub-telomeric regions, while lsd1-∆HMG clr3∆ double mutants have the most robust effect at the mating-type locus (Figure 7D). ------- COMMENT: 9b5edbe6f0efcb45 24 LujE2QqTS5L2GjlD+aZhr4mfm0w However, H3-K56R, rtt109Δ, and the H3-K56R/rtt109Δ mutant cells formed colonies of variable pink indicating a slight decrease in silencing at centromeres (Fig. 4A). This might indicate a cross-talk of H3 Lys-56-Ac with the establishment or the maintenance of other activating or repressing histone modifications required for proper centromeric heterochromatin formation. ------- COMMENT: 9b6d37d028f7da71 1 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 9b6d37d028f7da71 2 iRxQ8LNGDFWKZch0u5NXVcN1+yE Fig. 1D. The cells also presented a defect in the degradation of the cyclin Cdc13 and a delay in the dephosphorylation of Ste9 ------- COMMENT: 9b6d37d028f7da71 3 3oUK/FxWnUfhzon8g79+uOTqKac Fig. 2G. the use of cdc10 mutant backgrounds is common for checking the ability of cells to arrest in G1 ------- COMMENT: 9b6d37d028f7da71 4 iNxlIjTcX4tpnNc1FUVyNcP2wwk fig7A ------- COMMENT: 9b6d37d028f7da71 5 x5dxxUovdrdcGHUlBQOXH2mTm1I Delay in the dephosphoryaltion of Ste9 and defect in the degradation of the cyclin Cdc13 in nitrogen starvation ------- COMMENT: 9b6d37d028f7da71 11 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 9b6d37d028f7da71 12 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 9b6d37d028f7da71 13 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: 9b6d37d028f7da71 14 19wtgd4o8xRlMW+VlkJw08n7nLE fig S1A ------- COMMENT: 9b6d37d028f7da71 15 19wtgd4o8xRlMW+VlkJw08n7nLE fig S1A ------- COMMENT: 9b6d37d028f7da71 16 nK6HeFDYijcE/UXroa6RA29ukqU fig 1C (comment: i.e normal TOR signalloing) ------- COMMENT: 9b6d37d028f7da71 17 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: 9b6d37d028f7da71 18 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: 9b6d37d028f7da71 19 ndbSJFK5bAshJ399UHeR+p7vwAg fig 2 This conserved inhibitory phosphorylation occurs as the Cdc2-Cdc13 complex is being formed to prevent its premature activation during G2 phase ------- COMMENT: 9b6d37d028f7da71 20 L2xntQsyydRJH8G0d78lnSausGc fig 2D length is 7.5 micron cf WT 6.2 in same conditions ------- COMMENT: 9b6d37d028f7da71 21 fW/p1oMZfKJ5vh1RHX5kqlxlQXs fig 2D length is 10.299 micron cf WT 12.7 in same conditions ------- COMMENT: 9b6d37d028f7da71 22 ctBaHyYXdgMsNELpg1F9GZ8G7kY fig 2E, S2A ------- COMMENT: 9b6d37d028f7da71 24 NJjexYHvo5CfkN0JOfp+Ru2EdHk fig S2B ------- COMMENT: 9b6d37d028f7da71 25 x7T3PPddCwSF6Ljb6VMt6c/BeP4 fig 2F ------- COMMENT: 9b6d37d028f7da71 27 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 9b6d37d028f7da71 28 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: 9b6d37d028f7da71 29 x7T3PPddCwSF6Ljb6VMt6c/BeP4 fig 2F ------- COMMENT: 9b6d37d028f7da71 30 ltrQkoUJm6Wn2k5av1wL1F55HgU fig3b ------- COMMENT: 9b6d37d028f7da71 31 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: 9b6d37d028f7da71 32 JcBZNgnYXe+imHdYCpMT/ONAz2I deletion mutants of either ste9 or rum1 fail to degrade Cdc13 (Figures S4A and S4B). ------- COMMENT: 9b6d37d028f7da71 33 JcBZNgnYXe+imHdYCpMT/ONAz2I deletion mutants of either ste9 or rum1 fail to degrade Cdc13 (Figures S4A and S4B). ------- COMMENT: 9b6d37d028f7da71 34 +YmWZBtF4r6G00V18ie7Psg8Rn8 Figure S4D ------- COMMENT: 9b6d37d028f7da71 35 +YmWZBtF4r6G00V18ie7Psg8Rn8 Figure S4D ------- COMMENT: 9b6d37d028f7da71 36 +YmWZBtF4r6G00V18ie7Psg8Rn8 Figure S4D ------- COMMENT: 9b6d37d028f7da71 37 +YmWZBtF4r6G00V18ie7Psg8Rn8 Figure S4D ------- COMMENT: 9b6d37d028f7da71 38 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: 9b6d37d028f7da71 39 VH8rvWatr38c1U+cm6YZYHrqmwE fig 4 Notably, loss of cig1 and cig2 utterly overrode these defects, ------- COMMENT: 9b6d37d028f7da71 40 tpUGoIu6oqS6cFh01iuTuuGxMlo Deletion of par1 also affected the survival of the wee1-50 mutant (Figure 4C), and this worsening of the phenotype corre- lated with the inability of the double wee1-50 par1D mutant to accumulate Rum1 (Figure 4D). ------- COMMENT: 9b6d37d028f7da71 41 tpUGoIu6oqS6cFh01iuTuuGxMlo Deletion of par1 also affected the survival of the wee1-50 mutant (Figure 4C), and this worsening of the phenotype corre- lated with the inability of the double wee1-50 par1D mutant to accumulate Rum1 (Figure 4D). ------- COMMENT: 9b6d37d028f7da71 45 fYaqi3KQ/dydOqnzi8pTaIGWgLU (TAP-Par1F314Q), this interaction was reduced (Figure 6C) ------- COMMENT: 9b6d37d028f7da71 46 LeNTmZM165nThXD7k/juer9NLio (comment: protein phophatase substrate adaptor) ------- COMMENT: 9b6d37d028f7da71 47 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: 9b739862e4c2c98b 3 8ytXQRVjDTAsu6Irk+fgLFA+Dz4 assayed using ArgDHFRts–HA–Mcm4ts construct or AspRec8c–FGFP construct ------- COMMENT: 9b739862e4c2c98b 18 OBTclmL1wfUbWtTYLYGCRu828rQ also assayed using AspRec8c–FGFP construct, which persists longer than unmodified full-length Rec8 ------- COMMENT: 9b739862e4c2c98b 20 8ytXQRVjDTAsu6Irk+fgLFA+Dz4 assayed using ArgDHFRts–HA–Mcm4ts construct or AspRec8c–FGFP construct ------- COMMENT: 9b739862e4c2c98b 24 Bi+G9UaQgUmw7xCvwFe38ZyENEc assayed using AspRec8c–FGFP construct ------- COMMENT: 9b739862e4c2c98b 25 Bi+G9UaQgUmw7xCvwFe38ZyENEc assayed using AspRec8c–FGFP construct ------- COMMENT: 9b739862e4c2c98b 26 3wL5izHGGtiw4bMtksyy1ZLMlpM assayed using cell growth with AspRec8c–FGFP–Mei2SATA construct (degradation frees Mei2SATA to arrest cell cycle) ------- COMMENT: 9b7a8fcbcc0f3510 1 IxCFgq/5pnBFdhV8pGSoKso7nuo fig2B, Fig. S2B-D ------- COMMENT: 9b7a8fcbcc0f3510 2 IxCFgq/5pnBFdhV8pGSoKso7nuo fig2B, Fig. S2B-D ------- COMMENT: 9b7a8fcbcc0f3510 3 IxCFgq/5pnBFdhV8pGSoKso7nuo fig2B, Fig. S2B-D ------- COMMENT: 9b7a8fcbcc0f3510 4 IxCFgq/5pnBFdhV8pGSoKso7nuo fig2B, Fig. S2B-D ------- COMMENT: 9b7a8fcbcc0f3510 5 IxCFgq/5pnBFdhV8pGSoKso7nuo fig2B, Fig. S2B-D ------- COMMENT: 9b7a8fcbcc0f3510 6 IxCFgq/5pnBFdhV8pGSoKso7nuo fig2B, Fig. S2B-D ------- COMMENT: 9b7a8fcbcc0f3510 7 IxCFgq/5pnBFdhV8pGSoKso7nuo fig2B, Fig. S2B-D ------- COMMENT: 9b7a8fcbcc0f3510 23 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: 9b7a8fcbcc0f3510 24 4/plyOvcotX6m2wKjxi1geqxXe8 Figure 1B. ------- COMMENT: 9b7a8fcbcc0f3510 27 +w8GNksoF9uxaAZAbz60EV18e/8 37 C is a moderate heat shock for fission yeast, which does not slow growth nor exerts toxicity to wild-type cultures, but significantly affects the viability of cells lacking stress signaling components, such as the MAP kinase Sty1 (Figures 1E and S1B). ------- COMMENT: 9b7a8fcbcc0f3510 33 +BFm9WdI3pS7EJA0I5feL4/8wnk Fig S2A ------- COMMENT: 9b7a8fcbcc0f3510 34 JzyBKOIiyuH2U/GwaoWxC6/fJhQ Fig S2 ------- COMMENT: 9b7a8fcbcc0f3510 35 JzyBKOIiyuH2U/GwaoWxC6/fJhQ Fig S2 ------- COMMENT: 9b7a8fcbcc0f3510 36 JzyBKOIiyuH2U/GwaoWxC6/fJhQ Fig S2 ------- COMMENT: 9b7a8fcbcc0f3510 37 JzyBKOIiyuH2U/GwaoWxC6/fJhQ Fig S2 ------- COMMENT: 9b7a8fcbcc0f3510 38 baZ+XVLWmlHNTi2mxxw2d7DfAJw fig 3D) Lack of Mas5 abolishes both PAC formation and the assembly of stress granules. ------- COMMENT: 9b7a8fcbcc0f3510 39 WI5tAkcerjyhlsXweoj8DX9nnxU fig 4D ------- COMMENT: 9b7a8fcbcc0f3510 40 WI5tAkcerjyhlsXweoj8DX9nnxU fig 4D ------- COMMENT: 9bba111aad473ae7 1 XPnM32shLX68Eo2rR66Yrm1uKgQ fig 1c ------- COMMENT: 9bba111aad473ae7 2 RdPxKy6TA/oocuhfrRm1ufsavvc fig 1d ------- COMMENT: 9bba111aad473ae7 3 RdPxKy6TA/oocuhfrRm1ufsavvc fig 1d ------- COMMENT: 9bba111aad473ae7 4 KvJuNoZSSkf9bYRBmFEE/lSLr8w fig 1d (comment: CHECK this term should really be trafficing) ------- COMMENT: 9bc7b364a9234861 7 oaoiyXStbmH2sN6zbLJuCbKZIAU fig2d - number 7 and 8 ------- COMMENT: 9bc7b364a9234861 8 oaoiyXStbmH2sN6zbLJuCbKZIAU fig2d - number 7 and 8 ------- COMMENT: 9bc7b364a9234861 9 1mScjghnno5oh+804//pCkEsJgg The profile of pmt3􏰗 cells showed a decrease of the number of cells with 2C DNA content and an increase in the number of cells with a DNA content greater or less than 2C DNA content (at times 􏰔2 and 0 h in Fig. 3C) ------- COMMENT: 9bc7b364a9234861 10 1mScjghnno5oh+804//pCkEsJgg The profile of pmt3􏰗 cells showed a decrease of the number of cells with 2C DNA content and an increase in the number of cells with a DNA content greater or less than 2C DNA content (at times 􏰔2 and 0 h in Fig. 3C) ------- COMMENT: 9bc7b364a9234861 15 pi8tR/OzhaiNy9nAOGhKmN/XxhM Fig. 3c ------- COMMENT: 9bde74c8ee3e19f5 4 9lEf8lM9MfMtCeysJeqSQlOWrgE (comment: two-hybrid assay) ------- COMMENT: 9bde74c8ee3e19f5 7 9lEf8lM9MfMtCeysJeqSQlOWrgE (comment: two-hybrid assay) ------- COMMENT: 9bde74c8ee3e19f5 9 9lEf8lM9MfMtCeysJeqSQlOWrgE (comment: two-hybrid assay) ------- COMMENT: 9bde74c8ee3e19f5 10 9lEf8lM9MfMtCeysJeqSQlOWrgE (comment: two-hybrid assay) ------- COMMENT: 9c097ba571c89868 74 pOepbznoNJk9+Ik5druivHnVs8A (comment: occurs at LTR and ncRNA) ------- COMMENT: 9c097ba571c89868 75 uQncOM0SVl1OXX2wqYFcf7WC4i4 (comment: LTR and ncRNA) ------- COMMENT: 9c097ba571c89868 87 pOepbznoNJk9+Ik5druivHnVs8A (comment: occurs at LTR and ncRNA) ------- COMMENT: 9c097ba571c89868 88 pOepbznoNJk9+Ik5druivHnVs8A (comment: occurs at LTR and ncRNA) ------- COMMENT: 9c368b73d2d16728 10 ovfoQStNT8Duy+3GvNFoYIgdb70 Fig. 4E ------- COMMENT: 9c50d59be8aa45eb 15 keOF2M9ZAz2eOSv5OjXgC6Bm/ZM (comment: CHECK localization independent of actin cytoskeleton (assayed using latrunculin A) and microtubule cytoskeleton (assayed using carbendazim)) ------- COMMENT: 9c63dd7d54df2fb5 1 b4kEiwyLBOnr9Jly/xlNKMICVBs Of the TSA-sensitive HDACs, Clr3 was the only one that had an effect on pho1 expression, with its deletion resulting in increased pho1 mRNA levels (Figure 1B). ------- COMMENT: 9c63dd7d54df2fb5 2 NAfBSnPVIHiihiDvosY7ctVmvRM In contrast, deletion of TSA- insensitive sir2, which contributes to transcriptional silenc- ing at constitutive heterochromatin, had no effect on pho1 expression (Figure 1B, lane 5). ------- COMMENT: 9c63dd7d54df2fb5 3 hG11d4sMQXpWuKwzFXAVxxEetuQ This revealed that Clr3 indeed local- izes to the gene, particularly at the non-coding region (Fig- ure 1C). ------- COMMENT: 9c63dd7d54df2fb5 4 8QqhbWeWCXvKIfLMBsjzImLbjoU Northern blot (Supplementary Figure S1A). Compared to wild-type pho1 mRNA levels, a slight accumulation was de- tected for all strains. However, this is less pronounced than in clr3, indicating that Clr3 is unlikely to entirely depend on Clr2 or other components of SHREC for recruitment to pho1. ------- COMMENT: 9c63dd7d54df2fb5 5 8QqhbWeWCXvKIfLMBsjzImLbjoU Northern blot (Supplementary Figure S1A). Compared to wild-type pho1 mRNA levels, a slight accumulation was de- tected for all strains. However, this is less pronounced than in clr3, indicating that Clr3 is unlikely to entirely depend on Clr2 or other components of SHREC for recruitment to pho1. ------- COMMENT: 9c63dd7d54df2fb5 6 8QqhbWeWCXvKIfLMBsjzImLbjoU Northern blot (Supplementary Figure S1A). Compared to wild-type pho1 mRNA levels, a slight accumulation was de- tected for all strains. However, this is less pronounced than in clr3, indicating that Clr3 is unlikely to entirely depend on Clr2 or other components of SHREC for recruitment to pho1. ------- COMMENT: 9c63dd7d54df2fb5 7 3u+AolitiYnQyEW4fEglHZzdXDE (Figure 1D). We found that loss of Clr3 leads to in- creased Pol II levels upstream of the pho1 promoter and particularly across the gene body. ------- COMMENT: 9c63dd7d54df2fb5 8 tJIQHMHr7IiVEnUMeOUjP+8t1Z4 an increase in the lev- els of H3K14ac could be detected by ChIP-qPCR (Figure 1E); ------- COMMENT: 9c63dd7d54df2fb5 9 RUWSfq9OoCOaGFaEnCjTChdaZks Re- markably, this mutant showed an additive accumulation of pho1 mRNA levels (Figure 2A) and displayed a slow growth phenotype considerably more severe than either of the sin- gle mutants, clr3 or clr4 (Figure 2B). ------- COMMENT: 9c63dd7d54df2fb5 11 p0zUJInpsC1PCb3WD5E/xmgLoEM We found that, in the absence of non-coding transcription in a strain lacking the prt promoter (ncpro) (5), Clr3 recruitment to pho1 was re- duced (Figure 2C). ------- COMMENT: 9c63dd7d54df2fb5 12 aKunemArNcg80BiHt7Z1RSVuqlU H3K14ac levels were also increased in this strain (Figure 2D), similar to that seen in clr3 (Fig- ure 1E) ------- COMMENT: 9c63dd7d54df2fb5 13 nEfoVqy9wL4bj4NQ5TJlLXq2DZ8 as expected, northern blot analysis revealed no obvious additive effect compared to the ncpro single mu- tant (Figure 2E). ------- COMMENT: 9c63dd7d54df2fb5 15 W/flHIrs1aTcvNVF6z8kMtXM8fo Interestingly, elevated pho1 levels were observed in prt-1, prt-2, and prt-3 (Figure 3B, lanes 1– 4) suggesting that element(s) responsible for pho1 silencing are located within the 5′ part of prt. ------- COMMENT: 9c63dd7d54df2fb5 16 W/flHIrs1aTcvNVF6z8kMtXM8fo Interestingly, elevated pho1 levels were observed in prt-1, prt-2, and prt-3 (Figure 3B, lanes 1– 4) suggesting that element(s) responsible for pho1 silencing are located within the 5′ part of prt. ------- COMMENT: 9c63dd7d54df2fb5 17 W/flHIrs1aTcvNVF6z8kMtXM8fo Interestingly, elevated pho1 levels were observed in prt-1, prt-2, and prt-3 (Figure 3B, lanes 1– 4) suggesting that element(s) responsible for pho1 silencing are located within the 5′ part of prt. ------- COMMENT: 9c63dd7d54df2fb5 18 gH8XvHKtF/TG7ge1ehDZ4AfBuC8 No change in pho1 ex- pression could be detected for either prt-4 or prt-5 (Fig- ure 3B, lanes 5 and 6). ------- COMMENT: 9c63dd7d54df2fb5 19 gH8XvHKtF/TG7ge1ehDZ4AfBuC8 No change in pho1 ex- pression could be detected for either prt-4 or prt-5 (Fig- ure 3B, lanes 5 and 6). ------- COMMENT: 9c63dd7d54df2fb5 20 t0lwHD1r1ZLasA5Cz+FV1TiGXv4 In the case of prt-3, this phenotype is likely a result of lost Mmi1 recruitment since this mu- tant lacks the DSR motifs we previously mapped ((5,37), Figure 3A, Mmi1 CRAC). We tested Mmi1 recruitment to the prt locus in this mutant and it is indeed defective (data not shown). ------- COMMENT: 9c63dd7d54df2fb5 22 LTsPFCKxPY/3dKgiqZnI/6+aBWk . Interest- ingly, deletion of the H3K4 methyltransferase Set1 leads to derepression of pho1 (Figure 3C). In contrast, deletion of the H3K36 methyltransferase Set2 only had a minor effect on pho1 derepression, suggesting that the mechanism un- derlying pho1 silencing primarily relies on histone methy- lation by Set1 ------- COMMENT: 9c63dd7d54df2fb5 24 sOOrTOdly9jQ/qCswcE3MZXXW+g To directly assess whether this is the case and that the two proteins function as part of the same pathway we performed a Northern blot to compare a double mu- tant clr3set1 with the respective single mutants (Figure 3D). We found that the double mutant did not lead to higher pho1 derepression as compared to single set1, indicating that both proteins do indeed function in the same pathway. ------- COMMENT: 9c63dd7d54df2fb5 25 PQd45NJPW/araacZKgBG1mphQzU To test whether Clr3 recruitment depends on Set1 we per- formed ChIP-qPCR. In support of Set1 acting upstream of Clr3 we found the HDAC’s recruitment to the pho1 locus to be compromised in a set1 strain (Figure 3E). ------- COMMENT: 9c63dd7d54df2fb5 26 RfbShxqMrjeg37lNE7yYb/VMDh0 We found accumulation of tgp1 in the seb1-1 mutant compared to wild-type (Supplementary Figure S3A, compare lanes 1 and 2). ------- COMMENT: 9c63dd7d54df2fb5 27 pxXYE2pgtkSE1qurmKmk7u2g2hY Co- incident with Seb1 loss, tgp1 mRNA levels start to accrue (Supplementary Figure S3A, lane 13) ------- COMMENT: 9c63dd7d54df2fb5 28 y56A33oPYIlC0GTmafaSKVCh6RU Unlike at pho1, we found that deletion of clr3 has no discernible induction ef- fect on tgp1 ------- COMMENT: 9c63dd7d54df2fb5 29 DHTXbDwS298u/v/LfmX87H1ZDEA No detectable increase in either tgp1 or nc-tgp1 was observed in sir2, strains harbouring a clr6 mutation (Supplementary Figure S3A, lanes 6–8) ------- COMMENT: 9c63dd7d54df2fb5 30 DHTXbDwS298u/v/LfmX87H1ZDEA No detectable increase in either tgp1 or nc-tgp1 was observed in sir2, strains harbouring a clr6 mutation (Supplementary Figure S3A, lanes 6–8) ------- COMMENT: 9c63dd7d54df2fb5 31 7ZRQ22m3tP3KgCjaUOb9q88otLg . clr4, or the dou- ble mutant clr3clr4 (Supplementary Figure S3A, lane 4 and 5), also revealed no change in mRNA expression lev- els. These results indicate that unlike pho1, tgp1 repression ------- COMMENT: 9c63dd7d54df2fb5 32 7ZRQ22m3tP3KgCjaUOb9q88otLg . clr4, or the dou- ble mutant clr3clr4 (Supplementary Figure S3A, lane 4 and 5), also revealed no change in mRNA expression lev- els. These results indicate that unlike pho1, tgp1 repression ------- COMMENT: 9c63dd7d54df2fb5 33 B/xMeHyekNpWDSf9Ng/MzfC8uX4 In agreement with a previously demonstrated role for the nuclear exosome complex in the degradation of meiotic transcripts during mitosis, we observe increased levels of meu19 and meu31 in rrp6 (Figure 5B and C). ------- COMMENT: 9c63dd7d54df2fb5 34 B/xMeHyekNpWDSf9Ng/MzfC8uX4 In agreement with a previously demonstrated role for the nuclear exosome complex in the degradation of meiotic transcripts during mitosis, we observe increased levels of meu19 and meu31 in rrp6 (Figure 5B and C). ------- COMMENT: 9c63dd7d54df2fb5 35 fCucuN6Gfk0uYB/Rw8h8TDIn7ls However, mug14 is not affected in this exosome mutant, suggesting that the gene is likely to be regulated only at the transcrip- tional level (Figure 5a). ------- COMMENT: 9c76de107a5d2e55 30 3CDyDJKZQSp0ridXv35P5WPPZ+Y starts with longer telomeres than wild type, which then shorten ------- COMMENT: 9c941de80b158052 30 ouYWzQQ3aR7ddi+SPUg7bMnEShs especially at centromere; also at other regions where Ino80 complex normally binds ------- COMMENT: 9c941de80b158052 33 ewfspod7Bin5Lx5/B/NV8EarpfI (comment: CONDITION 30 degrees) ------- COMMENT: 9c941de80b158052 34 max4HEZNUxQSXwmzYVBOGtNvtHw (comment: CONDITION 25 degrees) ------- COMMENT: 9c941de80b158052 38 ouYWzQQ3aR7ddi+SPUg7bMnEShs (comment: especially at centromere; also at other regions where Ino80 complex normally binds) ------- COMMENT: 9cf15ade89e44016 1 RImhCAmBWqUXVvBBfpHPNZjT/CU Figure 1B ------- COMMENT: 9cf15ade89e44016 2 fI2mVM1XbhoLKLg+g0t+WoowkVo Figure 1A ------- COMMENT: 9cf15ade89e44016 3 p1rz26Ixeostfqw6pFdimcwMdJA Figure 1A and 1B ------- COMMENT: 9cf15ade89e44016 4 XgkJPKPzFeXFr4xWGwbuI8Z/1hI Figure 3 ------- COMMENT: 9cf15ade89e44016 7 XgkJPKPzFeXFr4xWGwbuI8Z/1hI Figure 3 ------- COMMENT: 9cf15ade89e44016 9 XgkJPKPzFeXFr4xWGwbuI8Z/1hI Figure 3 ------- COMMENT: 9cf15ade89e44016 11 XgkJPKPzFeXFr4xWGwbuI8Z/1hI Figure 3 ------- COMMENT: 9cf15ade89e44016 13 XgkJPKPzFeXFr4xWGwbuI8Z/1hI Figure 3 ------- COMMENT: 9cf15ade89e44016 15 XgkJPKPzFeXFr4xWGwbuI8Z/1hI Figure 3 ------- COMMENT: 9cf15ade89e44016 17 XgkJPKPzFeXFr4xWGwbuI8Z/1hI Figure 3 ------- COMMENT: 9cf15ade89e44016 19 XgkJPKPzFeXFr4xWGwbuI8Z/1hI Figure 3 ------- COMMENT: 9cf15ade89e44016 20 hZ8QNzJNllWTBSHosfmhKuBcba8 Figure 8D ------- COMMENT: 9cf15ade89e44016 21 hZ8QNzJNllWTBSHosfmhKuBcba8 Figure 8D ------- COMMENT: 9cf15ade89e44016 22 hZ8QNzJNllWTBSHosfmhKuBcba8 Figure 8D ------- COMMENT: 9cf15ade89e44016 23 hZ8QNzJNllWTBSHosfmhKuBcba8 Figure 8D ------- COMMENT: 9cf15ade89e44016 25 9fq2FUbl2kYKzWYwXMLBMZie9Ho Figure 8B ------- COMMENT: 9cf15ade89e44016 26 RTzqxu+qcZMlwHb25hmwfi3kBxk Figure 8A ------- COMMENT: 9cf15ade89e44016 28 UJWU69SPPzoy/8cvmeXI5qgYo64 Figure 8B ------- COMMENT: 9cf15ade89e44016 29 UJWU69SPPzoy/8cvmeXI5qgYo64 Figure 8B ------- COMMENT: 9cf15ade89e44016 30 WJc2ZBSDl9y+GaQ1rxRfC24Ic4w Figure 8E ------- COMMENT: 9cf15ade89e44016 31 WJc2ZBSDl9y+GaQ1rxRfC24Ic4w Figure 8E ------- COMMENT: 9cf15ade89e44016 32 DO8meEUBgbl8/NgcARM294FNj1U Figure 6A and 6C ------- COMMENT: 9cf15ade89e44016 33 5x7dmMl7XPCumpMBQi6q0lmO4b8 Figure 6A ------- COMMENT: 9cf15ade89e44016 34 P9pTlttdL9/3qvGWBFfoD+1AZWQ Figure 7A ------- COMMENT: 9cf15ade89e44016 35 P9pTlttdL9/3qvGWBFfoD+1AZWQ Figure 7A ------- COMMENT: 9cf15ade89e44016 37 hZ8QNzJNllWTBSHosfmhKuBcba8 Figure 8D ------- COMMENT: 9cf15ade89e44016 39 hZ8QNzJNllWTBSHosfmhKuBcba8 Figure 8D ------- COMMENT: 9cf15ade89e44016 43 tGe5tIr8g+sFV98HNga5ic6R5J0 (comment: CHECK Requested new term from Sequence Ontology: CArG-box) ------- COMMENT: 9cf15ade89e44016 44 hZ8QNzJNllWTBSHosfmhKuBcba8 Figure 8D ------- COMMENT: 9d08f7029974925e 6 DBXF1M0/AOAL0syhqRtIw2BJ2AI (comment: at time 0. they don't look at nitrogen starvation for very long, only 60 mins) ------- COMMENT: 9d4e23c125e5a359 7 ZfEKtTkzdrommMXKReI3D4Iz0DM (comment: unstressed cells) ------- COMMENT: 9d4e23c125e5a359 13 ZfEKtTkzdrommMXKReI3D4Iz0DM (comment: unstressed cells) ------- COMMENT: 9d4e23c125e5a359 15 ZfEKtTkzdrommMXKReI3D4Iz0DM (comment: unstressed cells) ------- COMMENT: 9d4e23c125e5a359 19 ZfEKtTkzdrommMXKReI3D4Iz0DM (comment: unstressed cells) ------- COMMENT: 9d4e23c125e5a359 21 ZfEKtTkzdrommMXKReI3D4Iz0DM (comment: unstressed cells) ------- COMMENT: 9d4e23c125e5a359 24 ZfEKtTkzdrommMXKReI3D4Iz0DM (comment: unstressed cells) ------- COMMENT: 9d4e23c125e5a359 26 ZfEKtTkzdrommMXKReI3D4Iz0DM (comment: unstressed cells) ------- COMMENT: 9d4e23c125e5a359 28 ZfEKtTkzdrommMXKReI3D4Iz0DM (comment: unstressed cells) ------- COMMENT: 9d4e23c125e5a359 29 ZfEKtTkzdrommMXKReI3D4Iz0DM (comment: unstressed cells) ------- COMMENT: 9d4e23c125e5a359 39 ZfEKtTkzdrommMXKReI3D4Iz0DM (comment: unstressed cells) ------- COMMENT: 9d4e23c125e5a359 43 ZfEKtTkzdrommMXKReI3D4Iz0DM (comment: unstressed cells) ------- COMMENT: 9d4e23c125e5a359 44 ZfEKtTkzdrommMXKReI3D4Iz0DM (comment: unstressed cells) ------- COMMENT: 9d4e23c125e5a359 48 ZfEKtTkzdrommMXKReI3D4Iz0DM (comment: unstressed cells) ------- COMMENT: 9d4e23c125e5a359 49 ZfEKtTkzdrommMXKReI3D4Iz0DM (comment: unstressed cells) ------- COMMENT: 9d4e23c125e5a359 51 ZfEKtTkzdrommMXKReI3D4Iz0DM (comment: unstressed cells) ------- COMMENT: 9d4e23c125e5a359 53 ZfEKtTkzdrommMXKReI3D4Iz0DM (comment: unstressed cells) ------- COMMENT: 9d4e23c125e5a359 54 ZfEKtTkzdrommMXKReI3D4Iz0DM (comment: unstressed cells) ------- COMMENT: 9d4e23c125e5a359 55 ZfEKtTkzdrommMXKReI3D4Iz0DM (comment: unstressed cells) ------- COMMENT: 9d547e188c364e28 4 2GA2svWLmHVKqBgxbHAcYOM+FUA boosts expression of the APC activator Fzr1/Mfr1 ------- COMMENT: 9d547e188c364e28 6 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: 9d547e188c364e28 10 7EN/W7J56AkhkjSK6NvdsePEkSk fig3c ------- COMMENT: 9d547e188c364e28 11 7EN/W7J56AkhkjSK6NvdsePEkSk fig3c ------- COMMENT: 9d547e188c364e28 14 rqsSDQjfQ5VN09DCESecgl7+zmQ binds chromatin at promoter, and phenotypes suggest this ------- COMMENT: 9d56e0cfc18f0d09 1 Rf0jwViWTjUJFLoz0lXgF8jmQlQ Fig. 2J ------- COMMENT: 9d56e0cfc18f0d09 2 mF68BQ92TsVsG6F9Jn4pbshZhF4 Fig. 1I and J ------- COMMENT: 9d56e0cfc18f0d09 3 K1/6gPRHPtmeLsRJXY2N2wAugPE Fig. 1C and D ------- COMMENT: 9d56e0cfc18f0d09 4 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 9d56e0cfc18f0d09 5 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 9d56e0cfc18f0d09 6 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 9d56e0cfc18f0d09 7 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 9d56e0cfc18f0d09 8 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 9d56e0cfc18f0d09 9 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 9d56e0cfc18f0d09 10 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 9d56e0cfc18f0d09 11 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: 9d56e0cfc18f0d09 12 Rf0jwViWTjUJFLoz0lXgF8jmQlQ Fig. 2J ------- COMMENT: 9d56e0cfc18f0d09 13 gLHEZS/RUqaKaX4QPSHqLuBpelQ Fig. 2I ------- COMMENT: 9d56e0cfc18f0d09 14 gLHEZS/RUqaKaX4QPSHqLuBpelQ Fig. 2I ------- COMMENT: 9d56e0cfc18f0d09 15 PtWz3QLtFNOk+hQdKIzxMwXnm5Q Fig. 3A and B ------- COMMENT: 9d56e0cfc18f0d09 16 Wqys6SwMR959PtPaBZJBiD3oqCg Fig. 3C and D ------- COMMENT: 9d56e0cfc18f0d09 17 9L6beT6Zl7a2c8STl7X/W6NQMLE Fig. 4C and D ------- COMMENT: 9d56e0cfc18f0d09 18 S2mSjF7Z4ojuYMjqY4fkfS9ipVI Fig. 4F and G ------- COMMENT: 9d56e0cfc18f0d09 19 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: 9d56e0cfc18f0d09 20 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 9d56e0cfc18f0d09 21 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: 9d56e0cfc18f0d09 22 /y/lT91toEt8826JxjLWsOWE/wk Fig. 5C and D ------- COMMENT: 9d56e0cfc18f0d09 23 /y/lT91toEt8826JxjLWsOWE/wk Fig. 5C and D ------- COMMENT: 9d56e0cfc18f0d09 24 PDDP3qNWaZBNS6EHtyKIhTv69IM Fig. 5E and F ------- COMMENT: 9d58ec3ae4a32b92 1 u2TuB59gADSFUIn8HhyMyuRtC6A spSir2 efficiently deacetylated an H4 peptide with acetyl-lysine at position 16 (AcK16) and an H3 peptide with acetyl-lysine at posi- tion 9 (AcK9), compared to H4 peptides with acetyl- lysine at positions 5 (AcK5), 8 (AcK8), and 12 (AcK12) (Figure 1B). ------- COMMENT: 9d58ec3ae4a32b92 2 u2TuB59gADSFUIn8HhyMyuRtC6A spSir2 efficiently deacetylated an H4 peptide with acetyl-lysine at position 16 (AcK16) and an H3 peptide with acetyl-lysine at posi- tion 9 (AcK9), compared to H4 peptides with acetyl- lysine at positions 5 (AcK5), 8 (AcK8), and 12 (AcK12) (Figure 1B). ------- COMMENT: 9d58ec3ae4a32b92 3 yBnXSI6SpARLuHlCnL75j23G0+8 In contrast, sir2 L(BglII)::ade6 cells formed white colonies, indicating loss of silencing of the reporter gene (Figure 2C). ------- COMMENT: 9d58ec3ae4a32b92 4 GQblzhyxhbeSSgDy9wYPj5tlDZ8 We observed a strong reduction in staining of sir2 compared to sir2 cells, which indicated a reduced rate of switching to the opposite mating type in sir2 cells (Figure 2E, column 1). ------- COMMENT: 9d58ec3ae4a32b92 5 NwPc+5Sfqk3BlntepwH12JCirq8 We combined the sir2 mutant with REII and found that the sir2 REII double mutant had a strong haploid meiosis phenotype (Figure 2E). ------- COMMENT: 9d58ec3ae4a32b92 6 cTBlLQ1iWr9lNB04Wv8BSAA+3vA Deletion of sir2 caused derepression of ura4 at both loci (Figure 2D). However, this effect was much stronger at the imr repeats than at the otr repeats. While sir2 cells carrying imr1R::ura4 did not form colonies on FOA-containing plates, mutant cells carrying otr1R::ura4 showed ap- preciable growth on FOA plates (Figure 2D). These re- sults indicated that Sir2 was required for silencing at the S. pombe centromeric DNA regions. ------- COMMENT: 9d58ec3ae4a32b92 7 cTBlLQ1iWr9lNB04Wv8BSAA+3vA Deletion of sir2 caused derepression of ura4 at both loci (Figure 2D). However, this effect was much stronger at the imr repeats than at the otr repeats. While sir2 cells carrying imr1R::ura4 did not form colonies on FOA-containing plates, mutant cells carrying otr1R::ura4 showed ap- preciable growth on FOA plates (Figure 2D). These re- sults indicated that Sir2 was required for silencing at the S. pombe centromeric DNA regions. ------- COMMENT: 9d58ec3ae4a32b92 8 naMDWoxunbUbx3ZiCk40QOASZeY Compared to wild-type cells, in sir2 cells, acetylation of K9 was increased by 16-, 11-, and 7-fold at the ura4 reporter inserted in the mating locus (Kint2::ura4), the inner (imr1R::ura4), and the outer centromeric repeats (otr1R::ura4), respectively (Figure 3A). ------- COMMENT: 9d58ec3ae4a32b92 9 naMDWoxunbUbx3ZiCk40QOASZeY Compared to wild-type cells, in sir2 cells, acetylation of K9 was increased by 16-, 11-, and 7-fold at the ura4 reporter inserted in the mating locus (Kint2::ura4), the inner (imr1R::ura4), and the outer centromeric repeats (otr1R::ura4), respectively (Figure 3A). ------- COMMENT: 9d58ec3ae4a32b92 10 naMDWoxunbUbx3ZiCk40QOASZeY Compared to wild-type cells, in sir2 cells, acetylation of K9 was increased by 16-, 11-, and 7-fold at the ura4 reporter inserted in the mating locus (Kint2::ura4), the inner (imr1R::ura4), and the outer centromeric repeats (otr1R::ura4), respectively (Figure 3A). ------- COMMENT: 9d58ec3ae4a32b92 11 naMDWoxunbUbx3ZiCk40QOASZeY Compared to wild-type cells, in sir2 cells, acetylation of K9 was increased by 16-, 11-, and 7-fold at the ura4 reporter inserted in the mating locus (Kint2::ura4), the inner (imr1R::ura4), and the outer centromeric repeats (otr1R::ura4), respectively (Figure 3A). ------- COMMENT: 9d58ec3ae4a32b92 12 naMDWoxunbUbx3ZiCk40QOASZeY Compared to wild-type cells, in sir2 cells, acetylation of K9 was increased by 16-, 11-, and 7-fold at the ura4 reporter inserted in the mating locus (Kint2::ura4), the inner (imr1R::ura4), and the outer centromeric repeats (otr1R::ura4), respectively (Figure 3A). ------- COMMENT: 9d58ec3ae4a32b92 13 P5nRKC9NJrgDHCk9pgu2KkzyQ8E ChIP analy- sis showed that the level of Swi6 associated with Kint2::ura4 was reduced 47-fold in sir2 compared to wild-type cells (Figure 3B). ------- COMMENT: 9d58ec3ae4a32b92 14 zawRPylUUgYAcTIFYLXHVMmkkGg Moreover, the level of Swi6 associated with imr1R::ura4 and otr1R::ura4 report- ers was reduced 8- and 3-fold, respectively, in sir2 compared to wild-type cells (Figure 3B). ------- COMMENT: 9d58ec3ae4a32b92 15 zawRPylUUgYAcTIFYLXHVMmkkGg Moreover, the level of Swi6 associated with imr1R::ura4 and otr1R::ura4 report- ers was reduced 8- and 3-fold, respectively, in sir2 compared to wild-type cells (Figure 3B). ------- COMMENT: 9d58ec3ae4a32b92 16 AHav1XWts0PaXPcrufjcp7zli8Q Histone H3-K9 methylation levels at Kint2::ura4 and imr1R::ura4 were strongly reduced in sir2 com- pared to sir2 cells (32- and 13-fold, respectively) (Figure 3B). ------- COMMENT: 9d58ec3ae4a32b92 17 MSMT/ust6zwpGsCno3ywFltm2iQ Consistent with its weaker effect on silencing of otr1R::ura4, deletion of sir2 had only a weak effect on methylation of this ura4 reporter (Figure 3B). ------- COMMENT: 9d58ec3ae4a32b92 18 MSMT/ust6zwpGsCno3ywFltm2iQ Consistent with its weaker effect on silencing of otr1R::ura4, deletion of sir2 had only a weak effect on methylation of this ura4 reporter (Figure 3B). ------- COMMENT: 9d76f3efe27ac062 2 q7yQ4AbC2bEj6KiCpphVWV06cCo (comment: dns) ------- COMMENT: 9d76f3efe27ac062 5 Li7d0DRo31XhhVS5YgLaqCvXbI4 I inferred new because it's asymmetric and we know sin is new ------- COMMENT: 9d76f3efe27ac062 6 Li7d0DRo31XhhVS5YgLaqCvXbI4 I inferred new because it's asymmetric and we know sin is new ------- COMMENT: 9d76f3efe27ac062 7 hjudDkhxGUCalt7kRvYfkn9rMpY After shift to restrictive temperature, cdc16-116 cells display two phenotypes termed type I and type II cells (Minet et al., 1979; Cerutti and Simanis, 1999). Type I cells have two nuclei and make multiple septa. Type II cells have a single nucleus and a septa. It has been proposed that the type II cells immediately septate again after division because they inherit the SPB that contains active Spg1p (Cerutti and Simanis, 1999). In support of this hypothesis, Sid1p was present at the SPB in type II cells (Figure 3A; see arrow). ------- COMMENT: 9d76f3efe27ac062 8 hjudDkhxGUCalt7kRvYfkn9rMpY After shift to restrictive temperature, cdc16-116 cells display two phenotypes termed type I and type II cells (Minet et al., 1979; Cerutti and Simanis, 1999). Type I cells have two nuclei and make multiple septa. Type II cells have a single nucleus and a septa. It has been proposed that the type II cells immediately septate again after division because they inherit the SPB that contains active Spg1p (Cerutti and Simanis, 1999). In support of this hypothesis, Sid1p was present at the SPB in type II cells (Figure 3A; see arrow). ------- COMMENT: 9d76f3efe27ac062 10 23A7gKYH++mwxpDaLPxFOcAYx6Y fig 3c ------- COMMENT: 9d76f3efe27ac062 11 W4egHp8nZtd5iNYpwU05IAEyjwY fig 3d ------- COMMENT: 9d76f3efe27ac062 13 aYtiIx2BJYyKTEHqZ59/H7QKaDM Figure4; TableI; datanotshown ------- COMMENT: 9d76f3efe27ac062 14 aYtiIx2BJYyKTEHqZ59/H7QKaDM Figure4; TableI; datanotshown ------- COMMENT: 9d76f3efe27ac062 15 aYtiIx2BJYyKTEHqZ59/H7QKaDM Figure4; TableI; datanotshown ------- COMMENT: 9d76f3efe27ac062 16 aYtiIx2BJYyKTEHqZ59/H7QKaDM Figure4; TableI; datanotshown ------- COMMENT: 9d76f3efe27ac062 17 aYtiIx2BJYyKTEHqZ59/H7QKaDM Figure4; TableI; datanotshown ------- COMMENT: 9d76f3efe27ac062 18 aYtiIx2BJYyKTEHqZ59/H7QKaDM Figure4; TableI; datanotshown ------- COMMENT: 9d76f3efe27ac062 19 TTE9jP4Ik5DfmsKxHhmM1Daq/JA Cdc7p cannot localize to the SPB(s) in cdc11 (Figure 4; Table I) ------- COMMENT: 9d76f3efe27ac062 20 Li7d0DRo31XhhVS5YgLaqCvXbI4 I inferred new because it's asymmetric and we know sin is new ------- COMMENT: 9d76f3efe27ac062 22 aYtiIx2BJYyKTEHqZ59/H7QKaDM Figure4; TableI; datanotshown ------- COMMENT: 9d76f3efe27ac062 23 aYtiIx2BJYyKTEHqZ59/H7QKaDM Figure4; TableI; datanotshown ------- COMMENT: 9d76f3efe27ac062 24 aYtiIx2BJYyKTEHqZ59/H7QKaDM Figure4; TableI; datanotshown ------- COMMENT: 9d76f3efe27ac062 25 aYtiIx2BJYyKTEHqZ59/H7QKaDM Figure4; TableI; datanotshown ------- COMMENT: 9d76f3efe27ac062 26 aYtiIx2BJYyKTEHqZ59/H7QKaDM Figure4; TableI; datanotshown ------- COMMENT: 9d76f3efe27ac062 30 MLm1/qI7V84E3L2P9+3RMCi9rK8 GFP±Sid1p signal was not observed at SPBs in these cells although faint nuclear signal was observed (Figure 7A). This suggests that some aspect of completion of mitosis is required in order for Sid1p to localize. ------- COMMENT: 9d76f3efe27ac062 33 5lMJAWkQLL3oiwhsmaQN9vqvccc Afterashifttorestrictivetemperaturefor50min to inactivate Cdc2p, cells could now be observed that were septating without undergoing anaphase (Figure 7E and F, see arrows). At this point, 96% (68/71) of cells displaying Sid1p signal at the SPB were septating without having undergone anaphase (Figure 7E and F, see arrows ------- COMMENT: 9d8a86ce7ba5c91b 31 mclwzuGxW0ZkWeXpSDkNVHiRi8U (comment: decreased overall) ------- COMMENT: 9d8e022526960ee2 6 J8SVlBDy+mbJ6DJtu2mk0DKUYJE (comment: CHECK throughout cell cycle, with peak at M/G1) ------- COMMENT: 9d9a265db15a87cd 1 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: 9d9a265db15a87cd 7 4O0rJt3G3c5u8iBioRL4h0NVJjY Fig 6E-G ------- COMMENT: 9d9a265db15a87cd 10 2a2WKSnjM07Fv8OBv7hx2O3lGyA happens during cellular resposne to BFA Fig 3A, Fig 4A-B, Fig 5B, Fig S3 ------- COMMENT: 9d9a265db15a87cd 14 PcD6G7VtXwb+xjON4bc+6c+jXzo Fig 1C, Fig 4 ------- COMMENT: 9d9a265db15a87cd 15 rIu3ZpVAlTat2JxBOyGp4dwYUp0 Fig 1C, Fig 6C, Fig 7C, Fig S5 ------- COMMENT: 9d9a265db15a87cd 16 VFkskau1qxV6L9SVWfIqbfw500g Fig 5A ------- COMMENT: 9d9a265db15a87cd 19 urOpmJ7V2cOTeJDX6yi2ZCNyVFA Fig 4D ------- COMMENT: 9d9a265db15a87cd 20 8Bfe9Cak+Lc8NE4KYnce9AOU+B8 Fig 4E ------- COMMENT: 9d9a265db15a87cd 21 7ORt3I/HK4LBUYz+zRL2eYwvW0w Fig 6C ------- COMMENT: 9d9a265db15a87cd 22 7ORt3I/HK4LBUYz+zRL2eYwvW0w Fig 6C ------- COMMENT: 9d9a265db15a87cd 23 t06dwlfQjN9Tx1OFuUhULmgZu5c Fig 6B ------- COMMENT: 9d9a265db15a87cd 24 7ORt3I/HK4LBUYz+zRL2eYwvW0w Fig 6C ------- COMMENT: 9d9a265db15a87cd 25 7ORt3I/HK4LBUYz+zRL2eYwvW0w Fig 6C ------- COMMENT: 9d9a265db15a87cd 26 t06dwlfQjN9Tx1OFuUhULmgZu5c Fig 6B ------- COMMENT: 9d9a265db15a87cd 27 FzPW38FvaNYcNujiUEn6O/THKyM Fig 6D ------- COMMENT: 9d9a265db15a87cd 28 wlfKELQf/IKYM3k8IjN+k4pjQ/8 Fig 7C ------- COMMENT: 9d9a265db15a87cd 29 wlfKELQf/IKYM3k8IjN+k4pjQ/8 Fig 7C ------- COMMENT: 9d9a265db15a87cd 36 PcD6G7VtXwb+xjON4bc+6c+jXzo Fig 1C, Fig 4 ------- COMMENT: 9dba3a4623d24c82 1 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 9dba3a4623d24c82 6 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 9dba3a4623d24c82 7 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 9dba3a4623d24c82 8 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 9dba3a4623d24c82 9 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 9dba3a4623d24c82 10 w/T9gFx19+Bp2juHCVUBdckYQtk We serendipitously discovered that fission yeast cells exhibit significant proliferation even at 38°C and 39°C, but not at 40°C, on agar medium supplemented with rapamycin, a TORC1-specific inhibitor [...] the fkh1 null mutant failed to grow at 39°C even in the presence of rapamycin. Figure 1 ------- COMMENT: 9dba3a4623d24c82 11 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 9dba3a4623d24c82 12 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 9dba3a4623d24c82 13 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 9dba3a4623d24c82 14 aHH98Y5slOMW7n/EjJb3XN+mOLs Figure 2 and 3 ------- COMMENT: 9dba3a4623d24c82 15 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 9dba3a4623d24c82 16 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 9dba3a4623d24c82 17 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 9dba3a4623d24c82 18 aHH98Y5slOMW7n/EjJb3XN+mOLs Figure 2 and 3 ------- COMMENT: 9dba3a4623d24c82 19 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 9dba3a4623d24c82 20 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 9dba3a4623d24c82 21 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 9dba3a4623d24c82 22 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 9dba3a4623d24c82 25 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 9dba3a4623d24c82 26 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 9dba3a4623d24c82 28 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 9dba3a4623d24c82 29 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 9dba3a4623d24c82 32 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 9dba3a4623d24c82 33 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 34 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 35 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 36 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 37 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 38 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 39 aHH98Y5slOMW7n/EjJb3XN+mOLs Figure 2 and 3 ------- COMMENT: 9dba3a4623d24c82 40 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 9dba3a4623d24c82 41 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 9dba3a4623d24c82 42 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 9dba3a4623d24c82 43 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 9dba3a4623d24c82 44 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 9dba3a4623d24c82 45 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 9dba3a4623d24c82 46 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 9dba3a4623d24c82 47 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 9dba3a4623d24c82 48 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 9dba3a4623d24c82 49 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 9dba3a4623d24c82 50 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 9dba3a4623d24c82 51 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 9dba3a4623d24c82 52 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 9dba3a4623d24c82 53 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 9dba3a4623d24c82 54 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 9dba3a4623d24c82 55 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 9dba3a4623d24c82 56 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 9dba3a4623d24c82 57 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 58 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 59 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 60 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 61 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 62 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 63 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 64 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 65 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: 9dba3a4623d24c82 73 82rmuE2qaElnGkj7kcgJKzGNVCs Fig. S7 ------- COMMENT: 9dba3a4623d24c82 74 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 9dba3a4623d24c82 75 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 9dba3a4623d24c82 76 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 9dba3a4623d24c82 77 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 9dba3a4623d24c82 78 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 9dba3a4623d24c82 79 82rmuE2qaElnGkj7kcgJKzGNVCs Fig. S7 ------- COMMENT: 9dba3a4623d24c82 80 82rmuE2qaElnGkj7kcgJKzGNVCs Fig. S7 ------- COMMENT: 9dba3a4623d24c82 81 82rmuE2qaElnGkj7kcgJKzGNVCs Fig. S7 ------- COMMENT: 9dba3a4623d24c82 82 82rmuE2qaElnGkj7kcgJKzGNVCs Fig. S7 ------- COMMENT: 9dba3a4623d24c82 83 82rmuE2qaElnGkj7kcgJKzGNVCs Fig. S7 ------- COMMENT: 9dba3a4623d24c82 84 82rmuE2qaElnGkj7kcgJKzGNVCs Fig. S7 ------- COMMENT: 9dba3a4623d24c82 85 82rmuE2qaElnGkj7kcgJKzGNVCs Fig. S7 ------- COMMENT: 9dba3a4623d24c82 86 82rmuE2qaElnGkj7kcgJKzGNVCs Fig. S7 ------- COMMENT: 9dba3a4623d24c82 87 82rmuE2qaElnGkj7kcgJKzGNVCs Fig. S7 ------- COMMENT: 9dba3a4623d24c82 88 82rmuE2qaElnGkj7kcgJKzGNVCs Fig. S7 ------- COMMENT: 9dba3a4623d24c82 89 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: 9dba3a4623d24c82 90 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 9dba3a4623d24c82 91 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: 9dba3a4623d24c82 93 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 9dba3a4623d24c82 94 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 9dba3a4623d24c82 95 xHgWiQGxcgBO0xJRn3WgCICGeX4 Figure S6 ------- COMMENT: 9dba3a4623d24c82 96 xHgWiQGxcgBO0xJRn3WgCICGeX4 Figure S6 ------- COMMENT: 9dba3a4623d24c82 97 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 9dba3a4623d24c82 98 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 9dba3a4623d24c82 99 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 9dba3a4623d24c82 100 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 9dba3a4623d24c82 101 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 9dba3a4623d24c82 102 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 9dba3a4623d24c82 103 df7aw2u5dlg2cVd9KKYGT9Wr0zU Table S4 ------- COMMENT: 9dba3a4623d24c82 104 df7aw2u5dlg2cVd9KKYGT9Wr0zU Table S4 ------- COMMENT: 9dba3a4623d24c82 105 df7aw2u5dlg2cVd9KKYGT9Wr0zU Table S4 ------- COMMENT: 9dba3a4623d24c82 106 df7aw2u5dlg2cVd9KKYGT9Wr0zU Table S4 ------- COMMENT: 9dba3a4623d24c82 107 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 108 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 109 X9Lza+wTmLq4ADVIIkcpplPt12U Tabls S2 ------- COMMENT: 9dba3a4623d24c82 110 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 111 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 112 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 113 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 114 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 115 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 116 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 117 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 118 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 119 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 120 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 121 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 122 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 123 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 124 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 125 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 126 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 127 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 128 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 129 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 130 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 131 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 132 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 133 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 134 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 135 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 136 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 137 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 138 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 139 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 140 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 141 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 142 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 143 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 144 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 145 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 146 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 147 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 148 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: 9dba3a4623d24c82 149 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 9dba3a4623d24c82 150 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 9dba3a4623d24c82 151 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 9dba3a4623d24c82 152 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: 9dba3a4623d24c82 153 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 9dba3a4623d24c82 154 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 9dba3a4623d24c82 155 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 9dba3a4623d24c82 156 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 9dba3a4623d24c82 157 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: 9dba3a4623d24c82 158 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: 9dba3a4623d24c82 159 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: 9dba3a4623d24c82 160 cLKKHHathQCT1JZTf0i7RHzTu8A Fig. 5F ------- COMMENT: 9dba3a4623d24c82 161 T44vR21/o+u/qtfx00leUPdjBlI Fig. 5G ------- COMMENT: 9dba3a4623d24c82 162 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 163 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 164 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 165 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 166 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 167 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 168 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 169 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 170 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 171 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 172 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 173 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 174 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 175 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 176 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 177 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 178 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 179 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 180 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 181 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 182 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 183 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 184 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 185 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 186 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 187 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 188 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 189 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 190 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 191 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 192 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 193 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 194 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 195 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 196 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 197 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 198 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 199 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 200 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 201 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 202 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 203 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 204 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 205 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 206 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 207 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 208 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 209 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 210 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 211 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 212 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 213 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 214 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 215 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 216 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 217 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 218 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 219 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 220 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 221 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 222 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 223 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 224 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 225 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 226 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 227 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 228 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 229 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 230 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 231 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 232 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 233 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 234 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 235 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 236 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 237 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 238 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 239 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 240 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 241 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 242 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 243 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 244 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 245 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 246 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 247 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 248 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 249 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 250 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 251 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 252 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 253 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 254 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 255 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 256 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: 9dba3a4623d24c82 257 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: 9dba3a4623d24c82 258 aHH98Y5slOMW7n/EjJb3XN+mOLs Figure 2 and 3 ------- COMMENT: 9dba3a4623d24c82 259 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 9dba3a4623d24c82 260 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 9dba3a4623d24c82 261 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 9dba3a4623d24c82 262 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 9dba3a4623d24c82 263 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 9dba3a4623d24c82 264 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 9dba3a4623d24c82 265 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 9dba3a4623d24c82 266 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 9dba3a4623d24c82 267 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 9dba3a4623d24c82 268 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: 9dba3a4623d24c82 269 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 9dba3a4623d24c82 270 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 9dba3a4623d24c82 271 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 9dba3a4623d24c82 272 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 9dba3a4623d24c82 273 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 9dba3a4623d24c82 274 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 9dba3a4623d24c82 275 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 9dba3a4623d24c82 276 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 9dba3a4623d24c82 277 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 9dba3a4623d24c82 278 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 9dba3a4623d24c82 279 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: 9dba3a4623d24c82 280 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 9dba3a4623d24c82 281 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 9dba3a4623d24c82 282 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 9dba3a4623d24c82 283 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 9dba3a4623d24c82 284 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 9dba3a4623d24c82 285 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 9dba3a4623d24c82 286 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: 9dba3a4623d24c82 287 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 9dba3a4623d24c82 288 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 9dba3a4623d24c82 289 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 9dba3a4623d24c82 290 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 9dba3a4623d24c82 291 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: 9dba3a4623d24c82 292 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 293 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 294 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 295 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 296 xLld+U4XtO1Yq5XJ/vA+lPgihjU Fig. 6D ------- COMMENT: 9dba3a4623d24c82 297 xLld+U4XtO1Yq5XJ/vA+lPgihjU Fig. 6D ------- COMMENT: 9dba3a4623d24c82 298 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 299 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 300 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 301 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 302 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 303 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 304 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 305 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 306 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 307 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 308 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 309 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 310 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: 9dba3a4623d24c82 311 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 9dba3a4623d24c82 312 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 9dba3a4623d24c82 313 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 9dba3a4623d24c82 314 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: 9dba3a4623d24c82 315 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 9dba3a4623d24c82 316 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 9dba3a4623d24c82 317 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 9dba3a4623d24c82 318 vZczsf7Z9Wep2Zjg5PatQRDbsXk Figure S4 ------- COMMENT: 9dc7446c41f69cf6 1 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 9dc7446c41f69cf6 2 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 9dc7446c41f69cf6 3 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 9dc7446c41f69cf6 4 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: 9dc7446c41f69cf6 5 LkGQQw8FQBPnHuF6zSR3Jquhnkc fig 1C ------- COMMENT: 9dc7446c41f69cf6 6 oxLUFWLWOtqMfkVqvM+kjut9kg0 fig 1 (3-4um normal metaphese lenght 2-2.5 um ------- COMMENT: 9dc7446c41f69cf6 12 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: 9dc7446c41f69cf6 13 7IzPVIuZhuKqIVjB4l/cExHiwb8 fig 2D ------- COMMENT: 9dc7446c41f69cf6 14 7IzPVIuZhuKqIVjB4l/cExHiwb8 fig 2D ------- COMMENT: 9dc7446c41f69cf6 15 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: 9dc7446c41f69cf6 17 HBL6UR1SGUpWge6L5BoeOY2F8pA S1 ------- COMMENT: 9dc7446c41f69cf6 18 HBL6UR1SGUpWge6L5BoeOY2F8pA S1 ------- COMMENT: 9dc7446c41f69cf6 19 HBL6UR1SGUpWge6L5BoeOY2F8pA S1 ------- COMMENT: 9dc7446c41f69cf6 20 HBL6UR1SGUpWge6L5BoeOY2F8pA S1 ------- COMMENT: 9dc7446c41f69cf6 21 fNpmaE7MG19qjD0+PlLVAROJUuU fig 5a ------- COMMENT: 9dc7446c41f69cf6 22 i4SAQZFitZ0PD6HyIHCmIL+frgE fig 5a (comment: ie wt like) ------- COMMENT: 9df2954cb30abd20 1 K5gjAB31akzzj+hu5zvvwaJJLRA figure1B ------- COMMENT: 9df2954cb30abd20 2 k7DxcEIbc7RprBs8K3u3n+JLKO4 figure1A ------- COMMENT: 9df2954cb30abd20 3 k7DxcEIbc7RprBs8K3u3n+JLKO4 figure1A ------- COMMENT: 9df2954cb30abd20 4 k7DxcEIbc7RprBs8K3u3n+JLKO4 figure1A ------- COMMENT: 9df2954cb30abd20 5 k7DxcEIbc7RprBs8K3u3n+JLKO4 figure1A ------- COMMENT: 9df2954cb30abd20 6 k7DxcEIbc7RprBs8K3u3n+JLKO4 figure1A ------- COMMENT: 9df2954cb30abd20 7 k7DxcEIbc7RprBs8K3u3n+JLKO4 figure1A ------- COMMENT: 9df2954cb30abd20 8 k7DxcEIbc7RprBs8K3u3n+JLKO4 figure1A ------- COMMENT: 9df2954cb30abd20 9 k7DxcEIbc7RprBs8K3u3n+JLKO4 figure1A ------- COMMENT: 9df2954cb30abd20 10 EpzXTL5F1NMbPe7/MaskRpLU338 figure 4 DAPI staining ------- COMMENT: 9df2954cb30abd20 11 0fkrIz/ZtVkDVeFqOWK9GOjn6gw figure 4C mini-chromosome Ch16 loss assay ------- COMMENT: 9df2954cb30abd20 12 H0MnaHI1FiMjP1CYNeqjyYIN72Q figure 5A ------- COMMENT: 9df2954cb30abd20 13 /pwpixLIYiIW7Ro7MdVcpbHinhA mini-chromosome Ch16 loss assay ------- COMMENT: 9df2954cb30abd20 17 w38mvcPb/q8JvvK8HI+i/zGhYu0 Mal3 (1-143) did not interact with Tip1 ------- COMMENT: 9df2954cb30abd20 18 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: 9df2954cb30abd20 19 QxyA7Kxt4u0SZftovXPYBddoxhU Fig 1D ------- COMMENT: 9df2954cb30abd20 20 QxyA7Kxt4u0SZftovXPYBddoxhU Fig 1D ------- COMMENT: 9df2954cb30abd20 21 Xj3hyO6vLoFEKeG2yaNYCB3qa2U Overexpression of Mal3 (1-308 full length) rescues the TBZ-sensitive phenotype of pka1∆ ------- COMMENT: 9df2954cb30abd20 27 3FLHrdo4jpfeSIzCerPzpKZZQfo Overexpression of Mal3 (1-143) rescues the TBZ-sensitive phenotype of pka1∆ ------- COMMENT: 9df2954cb30abd20 28 KpOgkyXcDQqUA61sEKG/m042fG0 Overexpression of Mal3 (1-197) rescues the TBZ-sensitive phenotype of pka1∆ ------- COMMENT: 9df2954cb30abd20 29 vY4vgUjnqqjLOllGdWDMIKXkhTo Overexpression of Mal3 (1-218) weakly rescues the TBZ-sensitive phenotype of pka1∆ ------- COMMENT: 9df2954cb30abd20 30 5Nre3QOTaTwUyS2o9XNknGA4eXI Overexpression of Mal3 (1-241) shows growth defect of pka1∆ ------- COMMENT: 9df2954cb30abd20 31 1zq3yfNePZUYrzR2PU6LDGIp5xs Overexpression of Mal3 (135-308) does not rescue the TBZ-sensitive phenotype of pka1∆ ------- COMMENT: 9df2954cb30abd20 32 7ZsIL/PdJsxY/bENzkKENf+oKgs Overexpression of Mal3 (Q89R) shows growth defect of pka1∆ ------- COMMENT: 9df2954cb30abd20 33 HACIZv2uGCf1kfapxaYhBe2jqIU Overexpression of Mal3 (Q89E) rescues the TBZ-sensitive phenotype of pka1∆ ------- COMMENT: 9df2954cb30abd20 34 zV9IYjZFbDsOrrpvd/XnZH8UPNk Overexpression of Mal3 (1-143 Q89R) rescues the TBZ-sensitive phenotype of pka1∆ ------- COMMENT: 9df2954cb30abd20 35 9jEOWpOU573ekQO1sXjsYUketgw Overexpression of Mal3 (1-143 Q89E) does not rescues the TBZ-sensitive phenotype of pka1∆ ------- COMMENT: 9df2954cb30abd20 36 WSnEQlQsdG8i43ZeY7Jmd9nfnjk Overexpression of Tip1 does not rescues the TBZ-sensitive phenotype of pka1∆ ------- COMMENT: 9df2954cb30abd20 37 XusWawr2gsXHbQ8inQKZR75M9mY Overexpression of Tea1 does not rescues the TBZ-sensitive phenotype of pka1∆ ------- COMMENT: 9df2954cb30abd20 38 GDrd6eFW1gl4Oj/774zbHgSYI5g Overexpression of Tea2 shows growth defect of pka1∆ ------- COMMENT: 9df2954cb30abd20 39 mNuS1oAH0gKPE2EbmTnk6OjDPWQ Overexpression of Alp7 does not rescues the TBZ-sensitive phenotype of pka1∆ ------- COMMENT: 9df2954cb30abd20 40 RptpjkcinKcFKiknr4n3Py91Dxc Overexpression of Alp14 does not rescues the TBZ-sensitive phenotype of pka1∆ ------- COMMENT: 9df2954cb30abd20 41 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 9df2954cb30abd20 46 wTidM/bvPAbDSaI5k3lcMen2Rls figure S2A ------- COMMENT: 9df2954cb30abd20 47 T7TKFiCgC/Wkl8l0s9QdUf6uz8o figure S2B ------- COMMENT: 9df2954cb30abd20 48 0+UzsUY4qwQZU3FRltAShZDDH0c figure 2B (comment: (two hybrid)) ------- COMMENT: 9df2954cb30abd20 49 ukWhci5DTBLYfW/9WJKT6tjYASM figure 2B (comment: (two hybrid)) ------- COMMENT: 9df2954cb30abd20 50 ukWhci5DTBLYfW/9WJKT6tjYASM figure 2B (comment: (two hybrid)) ------- COMMENT: 9df2954cb30abd20 51 ukWhci5DTBLYfW/9WJKT6tjYASM figure 2B (comment: (two hybrid)) ------- COMMENT: 9df2954cb30abd20 52 iBbX2sDkL6uH6Slmt1+LO2KaCk8 figure 2C ------- COMMENT: 9df2954cb30abd20 53 iBbX2sDkL6uH6Slmt1+LO2KaCk8 figure 2C ------- COMMENT: 9df2954cb30abd20 54 iBbX2sDkL6uH6Slmt1+LO2KaCk8 figure 2C ------- COMMENT: 9df2954cb30abd20 55 iBbX2sDkL6uH6Slmt1+LO2KaCk8 figure 2C ------- COMMENT: 9df2954cb30abd20 57 Mc1Dl/8ePjG+W3BQIevC1vaEvqw Figure 2D ------- COMMENT: 9df2954cb30abd20 58 Mc1Dl/8ePjG+W3BQIevC1vaEvqw Figure 2D ------- COMMENT: 9df2954cb30abd20 59 iyMraj5qmLEJtewo/fSEFxmllqQ figure 2D ------- COMMENT: 9df2954cb30abd20 60 iyMraj5qmLEJtewo/fSEFxmllqQ figure 2D ------- COMMENT: 9df2954cb30abd20 61 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 9df2954cb30abd20 62 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 9df2954cb30abd20 63 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 9df2954cb30abd20 66 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: 9df2954cb30abd20 67 POQ2C6IwAqJBwYWm3lz4xhY0ORA figure 4C ------- COMMENT: 9df2954cb30abd20 68 POQ2C6IwAqJBwYWm3lz4xhY0ORA figure 4C ------- COMMENT: 9df2954cb30abd20 69 H0MnaHI1FiMjP1CYNeqjyYIN72Q figure 5A ------- COMMENT: 9df2954cb30abd20 70 H0MnaHI1FiMjP1CYNeqjyYIN72Q figure 5A ------- COMMENT: 9df2954cb30abd20 71 H0MnaHI1FiMjP1CYNeqjyYIN72Q figure 5A ------- COMMENT: 9df2954cb30abd20 72 wTidM/bvPAbDSaI5k3lcMen2Rls figure S2A ------- COMMENT: 9df2954cb30abd20 73 T7TKFiCgC/Wkl8l0s9QdUf6uz8o figure S2B ------- COMMENT: 9df2954cb30abd20 74 H0MnaHI1FiMjP1CYNeqjyYIN72Q figure 5A ------- COMMENT: 9df2954cb30abd20 75 H0MnaHI1FiMjP1CYNeqjyYIN72Q figure 5A ------- COMMENT: 9e08a5b153885482 2 W5pOvN21FopBF79WgXMlLYHfGgw (comment: CHECK TERM REQUESTED growth auxotrophic for isoleucine) ------- COMMENT: 9e08a5b153885482 3 wegf+IfnsXqfR/evbaCCCHSjANg (comment: CHECK TERM REQUESTED growth auxotrophic for valine) ------- COMMENT: 9e29d45d34f5263a 13 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 9e29d45d34f5263a 15 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: 9e29d45d34f5263a 16 Kgp5LJbZ6dTebhOm5Q01+YvY76k mild over expression of cdc13+ on multi copy plasmid pYep13 rescues the cdc13-117 ts phenotype. . ------- COMMENT: 9e29d45d34f5263a 17 B5qltGIcvJ74CE8gbB98WMWZ+RQ mild over expression of cdc13+ on multi copy plasmid pYep13 causes slow growth ------- COMMENT: 9e29d45d34f5263a 18 6h5drxbzklmWSg1D+GXGlvwhdVQ mild over expression of cdc2+ on multi copy plasmid rescues the cdc13-117 ts phenotype ------- COMMENT: 9e6658619f3b13c3 7 +1M9TZOY1JcDGmE7uqD2lVqun8M Figure 4, A and B, and Supplemen- tal Movie S1 ------- COMMENT: 9e870ff297c803f8 1 HnxvlYyg5FOErZ0VhNxmDe19Brg ChIP ------- COMMENT: 9e870ff297c803f8 2 N6xHtZNvesaGZ48X7+5ElGz/Kn4 forms covalent linkage upon binding (wouldn't normally use ChIP as IDA for DNA binding MF, but the phenol extraction with or without protease adds more confidence) ------- COMMENT: 9e870ff297c803f8 3 E8BewJUPIW9Wx9mOCvREZALbkFI Southern blot ------- COMMENT: 9e870ff297c803f8 4 SmefE6t74HQlXgn5sJfmzuQDSAg microarray ------- COMMENT: 9e870ff297c803f8 5 E8BewJUPIW9Wx9mOCvREZALbkFI Southern blot ------- COMMENT: 9e870ff297c803f8 7 E8BewJUPIW9Wx9mOCvREZALbkFI Southern blot ------- COMMENT: 9e870ff297c803f8 8 E8BewJUPIW9Wx9mOCvREZALbkFI Southern blot ------- COMMENT: 9e870ff297c803f8 9 E8BewJUPIW9Wx9mOCvREZALbkFI Southern blot ------- COMMENT: 9e9172c11d4beefc 16 /8uR5ZPVNEhKSWLmBcpei43cbEA (comment: CHECK osmotic stress) ------- COMMENT: 9e9172c11d4beefc 17 /8uR5ZPVNEhKSWLmBcpei43cbEA (comment: CHECK osmotic stress) ------- COMMENT: 9ef4b740616f8870 12 cYoNcIXJEnjLr7sTmmxZa9aeK5w because slp1 can bypass wee1 it must independently inhibit cd2 ------- COMMENT: 9ef4b740616f8870 14 qICgub4puyvEhuhT3k5UQOKAbHM (comment: CHECK add penetrance?) ------- COMMENT: 9ef4b740616f8870 29 fNpmaE7MG19qjD0+PlLVAROJUuU fig 5a ------- COMMENT: 9ef4b740616f8870 32 1uc7ZgaD3vfwg2cJItcWc1Jpqyw fig 5c (comment: switched from conjugtion freqeuncy to sterility as can only capture penetance on cell phenotypes) ------- COMMENT: 9ef4b740616f8870 35 EmYuYkrwrNxWA1tAWsMmK7MX03A (comment: nitrogen induced arrest) ------- COMMENT: 9ef4b740616f8870 36 e0wmRVwMRbf7qUGt26hC82zTV/g (comment: G1 phase nitrogen induced arrest) ------- COMMENT: 9ef4b740616f8870 37 cYoNcIXJEnjLr7sTmmxZa9aeK5w because slp1 can bypass wee1 it must independently inhibit cd2 ------- COMMENT: 9ef4c870fd50a8b6 1 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: 9ef4c870fd50a8b6 2 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: 9ef4c870fd50a8b6 3 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: 9ef4c870fd50a8b6 4 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: 9ef8610874465dee 1 7NEba+HhzRjSNOGEoHIyulwi2mU (Figure 7) rec8-F204S mutant is defective in LinE formation and recombination (Rec10-mCherry forms aberrant dotty or filamentous aggregates within the nucleus, similar to rec8∆.) ------- COMMENT: 9ef8610874465dee 2 7nXZEfrbVGKlinshQMv1L0+aOlI (Figure 7) rec8-F204S mutant is defective in LinE formation and recombination ------- COMMENT: 9ef8610874465dee 3 E5LiFE6jPwnnJa55PltTxqauud4 (Figure 5C) The rec8-F204S mutant maintained sister chromatid cohesion as assessed at the cut3 gene locus ------- COMMENT: 9ef8610874465dee 4 /nBJmt6zTnXsd6xwa/K0QhCAVQs (Figure 6B). The alignment index of chromosome 1 at meiotic prophase (2.5 h) decreased in rec8-F204S (1.6) compared with the wild-type (rec8-wt, 2.6) ------- COMMENT: 9ef8610874465dee 5 ecG9iJYKwZlm374BpDtd4C/XAzA (Figure 7) ------- COMMENT: 9ef8610874465dee 6 +DOCCQVmxYgJnquQBi1bj9BfBts (Figure 5D, Supplemental Movies 1, and 3) In the rec8-F204S mutant, only the leading edge of the nucleus followed the horsetail movement, while the bulk of chromosomes were left behind, similar to rec8Δ ------- COMMENT: 9ef8610874465dee 7 CgRsGGkxrNu9x7G4fZykZx8cV3k (comment: DECREASED NUMBER) (figure 6 and figure 5e, S6BC) defective loop formation also supported by increased distance between the telomere-ade8 distance was longer in the rec8-F204S mutant than in the wild-type, suggesting that the chromatin of the rec8-F204S mutant was flexible and was abnormally stretched by the traction of the horsetail movement ------- COMMENT: 9ef8610874465dee 8 pwYo4qq3zpJvUEZsuqNj7EY7jzA Figure S4B ------- COMMENT: 9ef8610874465dee 11 rNYASkWOIaI050onJx0D65YEGZc figure 4c,e also supported by (Figure 4A), Fluorescence images of Rec8- GFP showed that axial structures in meiotic chromosomes were more prominent in wpl1Δ than in the wild-type ------- COMMENT: 9ef8610874465dee 12 DbDQ1txSZWVJs9Jc3J889zOSG7M Supplementary Figure S4B) wpl1Δ rarely showed torsional turning (Supplementary Figure S4C, Supplemen- tal Movies 1, and 2) during horsetail movements that is important for the alignment of homologs ------- COMMENT: 9ef8610874465dee 13 5DmH2gSwPSD0skgpo/CI0EMbLk8 (Figures 2A and B) Hi-C analysis for rec10Δ and rec12Δ cells showed X-shaped contacts similar to wild-type cells AND (Figure 3A).... punctate Hi-C interactions observed in the wild-type were mostly lost in rec8Δ ------- COMMENT: 9ef8610874465dee 14 ZugkGhcYQElsFj/b7/RZxDxhhjc (Figures 2A and B) Hi-C analysis for rec10Δ and rec12Δ cells showed X-shaped contacts similar to wild-type cells AND (Figure 3A).... punctate Hi-C interactions observed in the wild-type were mostly lost in rec8Δ ------- COMMENT: 9ef8610874465dee 19 2gutc7Arw8OVy8HbleCZRcd/bH0 These results suggest that Wpl1 plays a role in alignment of homologs through Rec8-dependent formation of axis-loop chromatin structure. ------- COMMENT: 9ef8610874465dee 22 B/aRND74BLV7rTuFjLSB+Da8Zug rec8-S552P and rec8Δ, which showed the cohesion defect, were used as a control strain (see Supplementary Figure S5C, S5D, and S5E for details of the rec8-S552P mutant) ------- COMMENT: 9ef8610874465dee 23 c1LzqEMwgo9nia5r8q0utw+fYZE These results sugest that in the rec8-F204S mutant, as in rec8Δ, the Rec8- dependent meiosis-specific short chromatin loop structures are lost, resulting in a concomitant loss of the structural property of the chromosome required for proper alignment. ------- COMMENT: 9ef8610874465dee 28 f9TMfP4DaQk6htD1dFDHRe7OlqA (comment: CHECK during horsetail/ prophase) ------- COMMENT: 9ef8610874465dee 29 I4mc9xgiYVJ608jmJLOCFeIjpX8 (Figure S5B) ------- COMMENT: 9ef8610874465dee 30 I4mc9xgiYVJ608jmJLOCFeIjpX8 (Figure S5B) ------- COMMENT: 9ef8610874465dee 31 2gutc7Arw8OVy8HbleCZRcd/bH0 These results suggest that Wpl1 plays a role in alignment of homologs through Rec8-dependent formation of axis-loop chromatin structure. ------- COMMENT: 9f0301dcfc800f2a 1 R80bIZbJxVvzq1jsaR2u5plUYbQ Cells lacking SPNCRNA.1343 (ncRNA.1343 for short) displayed a phenotype: hypersensitivity to TBZ, HU and caffeine but not to temperature extremities, ultraviolet- irradiation or oxidative stress (Supplementary Fig. 1c and Supplementary Fig. 2). ------- COMMENT: 9f0301dcfc800f2a 4 uGPrLIZeDnNhDADtteFC0G7bu9M (repressed condition; Fig. 1c) ------- COMMENT: 9f0301dcfc800f2a 5 a1xmQruQRwIeEItJDM7W+5Uf8u8 To determine whether the drug sensitivity of 1343D cells is a direct result of increased tgp1þ expression, the tgp1þ gene was deleted from 1343D cells (tgp1D1343D). This manipulation restored TBZ, HU and caffeine tolerance to levels comparable with wild-type cells (Fig. 1d). We conclude that increased tgp1þ expression is directly responsible for the drug-sensitivity phenotype of cells lacking ncRNA.1343. ------- COMMENT: 9f0301dcfc800f2a 8 8qO5rypduCkUSgkWRblDUdIrmWI The size and levels of the nc-1343 transcript increased in exosome defective (rrp6D) cells, but not cells lacking Mmi1 or Red1 (Fig. 2c,d; Supplementary Fig. 4). T ------- COMMENT: 9f0301dcfc800f2a 9 8qO5rypduCkUSgkWRblDUdIrmWI The size and levels of the nc-1343 transcript increased in exosome defective (rrp6D) cells, but not cells lacking Mmi1 or Red1 (Fig. 2c,d; Supplementary Fig. 4). T ------- COMMENT: 9f0301dcfc800f2a 10 FyyabeACOv7aHI79VbdyYPnoChI We identified a consensus DSR motif for Mmi1 binding at position þ820 nt within the nc-tgp1 transcript and RNA IP (RIP) experiments confirmed a direct interaction between Mmi1 and the nc-tgp1 RNA (Supplementary Fig. 5). ------- COMMENT: 9f0301dcfc800f2a 11 x+AAuK1eS8s5sI/tgRXuV+GiaCU Northern analysis identified that an B1.9kb nc-tgp1 RNA accumulates in rrp6D, mmi1D and red1D, but not in wild-type cells (Fig. 2e,f; Supplementary Fig. 4). ------- COMMENT: 9f0301dcfc800f2a 12 x+AAuK1eS8s5sI/tgRXuV+GiaCU Northern analysis identified that an B1.9kb nc-tgp1 RNA accumulates in rrp6D, mmi1D and red1D, but not in wild-type cells (Fig. 2e,f; Supplementary Fig. 4). ------- COMMENT: 9f0301dcfc800f2a 13 x+AAuK1eS8s5sI/tgRXuV+GiaCU Northern analysis identified that an B1.9kb nc-tgp1 RNA accumulates in rrp6D, mmi1D and red1D, but not in wild-type cells (Fig. 2e,f; Supplementary Fig. 4). ------- COMMENT: 9f0301dcfc800f2a 14 p+q4NENGTJlAhiTeOKrGdndtMnM Trun- cations of nc-1343 (that is, AD and BD) that retain its 50 end did not result in the drug-sensitivity phenotype presented by 1343D cells (Fig. 3b) and, similarly, did not induce tgp1þ expression (Fig. 3c) ------- COMMENT: 9f0301dcfc800f2a 18 p+q4NENGTJlAhiTeOKrGdndtMnM Trun- cations of nc-1343 (that is, AD and BD) that retain its 50 end did not result in the drug-sensitivity phenotype presented by 1343D cells (Fig. 3b) and, similarly, did not induce tgp1þ expression (Fig. 3c) ------- COMMENT: 9f0301dcfc800f2a 22 XBGaoSIzhBmjUBqRHuXROUCJpZM prevented nc-tgp1 transcription, induced tgp1þ expression to levels observed in 1343D levels and increased sensitivity of these cells to TBZ, HU and caffeine (Fig. 3b,c). These analyses demonstrate that it is nc-tgp1, not nc-1343, that is critical for repressing tgp1þ in the presence of phosphate. ------- COMMENT: 9f0301dcfc800f2a 28 9MGLgYFH6Nw5VPJlyt+hhDwiQRk In agreement with this, significantly less RNAPII associates with the nc-tgp1 transcription unit in both phosphate- starved wild-type cells and phosphate-replete 1343D cells, which do not transcribe nc-tgp1 (Fig. 4c). ------- COMMENT: 9f0301dcfc800f2a 29 8qO5rypduCkUSgkWRblDUdIrmWI The size and levels of the nc-1343 transcript increased in exosome defective (rrp6D) cells, but not cells lacking Mmi1 or Red1 (Fig. 2c,d; Supplementary Fig. 4). T ------- COMMENT: 9f0301dcfc800f2a 30 Tgvurqq508xruGkCzaFlqno5IuI In addition, the transcript levels of tgp1þ, nc-tgp1, nc-1343, pho1þ and nc-pho1 were unaffected by loss of RNAi (for example, ago1D or dcr1D) or heterochromatin components (for example, clr4D or swi6D) (Fig. 5b; Supplementary Fig. 7a ------- COMMENT: 9f0301dcfc800f2a 34 Tgvurqq508xruGkCzaFlqno5IuI In addition, the transcript levels of tgp1þ, nc-tgp1, nc-1343, pho1þ and nc-pho1 were unaffected by loss of RNAi (for example, ago1D or dcr1D) or heterochromatin components (for example, clr4D or swi6D) (Fig. 5b; Supplementary Fig. 7a ------- COMMENT: 9f0301dcfc800f2a 35 Tgvurqq508xruGkCzaFlqno5IuI In addition, the transcript levels of tgp1þ, nc-tgp1, nc-1343, pho1þ and nc-pho1 were unaffected by loss of RNAi (for example, ago1D or dcr1D) or heterochromatin components (for example, clr4D or swi6D) (Fig. 5b; Supplementary Fig. 7a ------- COMMENT: 9f0301dcfc800f2a 36 Tgvurqq508xruGkCzaFlqno5IuI In addition, the transcript levels of tgp1þ, nc-tgp1, nc-1343, pho1þ and nc-pho1 were unaffected by loss of RNAi (for example, ago1D or dcr1D) or heterochromatin components (for example, clr4D or swi6D) (Fig. 5b; Supplementary Fig. 7a ------- COMMENT: 9f0301dcfc800f2a 37 Tgvurqq508xruGkCzaFlqno5IuI In addition, the transcript levels of tgp1þ, nc-tgp1, nc-1343, pho1þ and nc-pho1 were unaffected by loss of RNAi (for example, ago1D or dcr1D) or heterochromatin components (for example, clr4D or swi6D) (Fig. 5b; Supplementary Fig. 7a ------- COMMENT: 9f0301dcfc800f2a 38 Jhb1eIDHtTJ67zVJHoK83Ev8VDQ In contrast, nc-tgp1, nc-pho1 and sme2þ RNA levels were clearly elevated in cells lacking Mmi1-mediated exosome degradation (mmi1D and rrp6D). Th ------- COMMENT: 9f0301dcfc800f2a 40 /WbKe6DUjVM1sHemkaHURk81kQ4 Our ChIP analyses confirmed that Pho7–green fluorescent protein (Pho7–GFP) accumulates on the pho1 þ promoter in phosphate- depleted cells (Supplementary Fig. 8). In addition, Pho7–GFP levels accumulate over the region upstream of tgp1þ when activated (Fig. 6a ------- COMMENT: 9f0301dcfc800f2a 41 hMzvi0VIYtlDQjViCGHinD0soMo We therefore conclude that tgp1(+) is regulated by transcriptional interference. ------- COMMENT: 9f1403044bcde8c5 1 vbReqaNkaFP3nnr8Jzp1Fct3JKw disruption of ckb1 alleviated the silencing of marker genes inserted in centromeric heterochromatin (Fig. 1A,B). ------- COMMENT: 9f1403044bcde8c5 2 307//g1DPg3G8eot0JFDRNkyNyI The extent of the silencing defects in ckb1D cells was comparable with that caused by disruption of other heterochromatic genes, including clr4, a histone H3K9-specific histone methyltransferase; clr3, a histone deacetylase and a subunit of SHREC; and swi6, a HP1 homolog (Fig. 1B). ------- COMMENT: 9f1403044bcde8c5 3 307//g1DPg3G8eot0JFDRNkyNyI The extent of the silencing defects in ckb1D cells was comparable with that caused by disruption of other heterochromatic genes, including clr4, a histone H3K9-specific histone methyltransferase; clr3, a histone deacetylase and a subunit of SHREC; and swi6, a HP1 homolog (Fig. 1B). ------- COMMENT: 9f1403044bcde8c5 4 307//g1DPg3G8eot0JFDRNkyNyI The extent of the silencing defects in ckb1D cells was comparable with that caused by disruption of other heterochromatic genes, including clr4, a histone H3K9-specific histone methyltransferase; clr3, a histone deacetylase and a subunit of SHREC; and swi6, a HP1 homolog (Fig. 1B). ------- COMMENT: 9f1403044bcde8c5 5 hR0jpZnDfFqOg/+xwy4RkzbUvLw Both H3K9me and Swi6 were enriched on centromeric repeats (dg223) in ckb1D cells similar to wild-type or clr3D cells (Fig. 1C). ------- COMMENT: 9f1403044bcde8c5 6 hR0jpZnDfFqOg/+xwy4RkzbUvLw Both H3K9me and Swi6 were enriched on centromeric repeats (dg223) in ckb1D cells similar to wild-type or clr3D cells (Fig. 1C). ------- COMMENT: 9f1403044bcde8c5 7 6BYwtUwGJ3nmU3uTOJNcDuUCpxs In contrast, levels of H3K9me and Swi6 at imr::ura4 were significantly decreased in clr3D and ckb1D cells (Fig. 1C). ------- COMMENT: 9f1403044bcde8c5 9 RHoQPGxo/FBnQuqH2a2c7e8SpyU this result suggests that Ckb1 is involved in the spreading of heterochromatin. (in ~GO, spreading is included in formation) ------- COMMENT: 9f1403044bcde8c5 10 bXP73MHXJodZ4fj4SGHXDAMUXwc H3K9me on the ura4 inserted at the mating locus heterochromatin (Kint2 ::ura4) was hardly affected by deletion of either atf1 or dcr1, an essential component of the RNAi-directed path- way (Fig. 1D). ------- COMMENT: 9f1403044bcde8c5 11 bXP73MHXJodZ4fj4SGHXDAMUXwc H3K9me on the ura4 inserted at the mating locus heterochromatin (Kint2 ::ura4) was hardly affected by deletion of either atf1 or dcr1, an essential component of the RNAi-directed path- way (Fig. 1D). ------- COMMENT: 9f1403044bcde8c5 12 CQsL7EWMQ4mdbc4isf/eUkHTIi8 Similarly, H3K9me on Kint2::ura4 was diminished in dcr1Dckb1D but still retained in atf1Dckb1D cells (Fig. 1D). T ------- COMMENT: 9f1403044bcde8c5 13 CQsL7EWMQ4mdbc4isf/eUkHTIi8 Similarly, H3K9me on Kint2::ura4 was diminished in dcr1Dckb1D but still retained in atf1Dckb1D cells (Fig. 1D). T ------- COMMENT: 9f1403044bcde8c5 14 UHsgcbIZRzvlScLGAMrEORY/d/k Therefore, Clr3 and Ckb1 function similarly in Atf1/Pcr1-dependent heterochromatin formation at the mating locus. ------- COMMENT: 9f1403044bcde8c5 15 UHsgcbIZRzvlScLGAMrEORY/d/k Therefore, Clr3 and Ckb1 function similarly in Atf1/Pcr1-dependent heterochromatin formation at the mating locus. ------- COMMENT: 9f1403044bcde8c5 16 Dl6/V6eFcOblYEryz7sOV7t7AoY Similarly, CK2 efficiently phos- phorylated bacteria-prepared Swi6 in a Ckb1-dependent manner, resulting in slower migrating bands in SDS- PAGE (Fig. 2C). ------- COMMENT: 9f1403044bcde8c5 17 MQm/Z2JYruZD5Vadd7/zSs+g+ow In addition, we found that the under- phosphorylated form of Swi6 was coprecipitated with Cka1-Flag (Fig. 2D), ------- COMMENT: 9f1403044bcde8c5 18 1nWjXQprN0g668V8jKocBdQFSYw whereas mutant Swi6 harboring S18A and S24A showed a slight mobility change, ------- COMMENT: 9f1403044bcde8c5 19 BGNdG6jSVouK3VWwjtwV3xNqIiI Mutations at the five CK2 sites located in the N-terminal half (S18-117A in Fig. 3A) resulted in an increase in mobility, ------- COMMENT: 9f1403044bcde8c5 20 xqsDbzrkJpJK2AUcmVdluw04Osk The lesser mobility change in ckb1D cells than that of Swi6-S18-117A would reflect the partial phosphorylation of Swi6 by residual activity of CK2 in ckb1D cells (Fig. 2A,C). ------- COMMENT: 9f1403044bcde8c5 21 ed50Cv31gkNrVtuMNOGwbMdc1pw Conversely, mutations at the five CK2 sites located in the C-terminal region of Swi6 (Fig. 3A) (swi6-S192-274A) did not influence the mobility of Swi6 (Fig. 3B). ------- COMMENT: 9f1403044bcde8c5 22 GCifHz/MBieYC0Cmbu5zV6mbfwA Mass spectrometric analysis of Swi6 prepared from wild-type and ckb1D cells showed that five potential CK2 phosphorylation sites (S18, S24, S46, S52, and S117) in the N-terminal half are really phosphorylated in vivo, and the phosphorylation of four of them (S18, S24, S46, and S117) decreased in ckb1D cells (Supplemental Fig. S5). ------- COMMENT: 9f1403044bcde8c5 23 GCifHz/MBieYC0Cmbu5zV6mbfwA Mass spectrometric analysis of Swi6 prepared from wild-type and ckb1D cells showed that five potential CK2 phosphorylation sites (S18, S24, S46, S52, and S117) in the N-terminal half are really phosphorylated in vivo, and the phosphorylation of four of them (S18, S24, S46, and S117) decreased in ckb1D cells (Supplemental Fig. S5). ------- COMMENT: 9f1403044bcde8c5 24 GCifHz/MBieYC0Cmbu5zV6mbfwA Mass spectrometric analysis of Swi6 prepared from wild-type and ckb1D cells showed that five potential CK2 phosphorylation sites (S18, S24, S46, S52, and S117) in the N-terminal half are really phosphorylated in vivo, and the phosphorylation of four of them (S18, S24, S46, and S117) decreased in ckb1D cells (Supplemental Fig. S5). ------- COMMENT: 9f1403044bcde8c5 25 GCifHz/MBieYC0Cmbu5zV6mbfwA Mass spectrometric analysis of Swi6 prepared from wild-type and ckb1D cells showed that five potential CK2 phosphorylation sites (S18, S24, S46, S52, and S117) in the N-terminal half are really phosphorylated in vivo, and the phosphorylation of four of them (S18, S24, S46, and S117) decreased in ckb1D cells (Supplemental Fig. S5). ------- COMMENT: 9f1403044bcde8c5 26 GCifHz/MBieYC0Cmbu5zV6mbfwA Mass spectrometric analysis of Swi6 prepared from wild-type and ckb1D cells showed that five potential CK2 phosphorylation sites (S18, S24, S46, S52, and S117) in the N-terminal half are really phosphorylated in vivo, and the phosphorylation of four of them (S18, S24, S46, and S117) decreased in ckb1D cells (Supplemental Fig. S5). ------- COMMENT: 9f1403044bcde8c5 28 2umfXJM+5r7rUvIrldfW0F1U+XY swi6-S192-274A mutations did not affect silencing at the centromere (imr::ura4), while swi6-S18-24A and S18- 117A mutants displayed decreases in silencing (Fig. 3C). ------- COMMENT: 9f1403044bcde8c5 29 2umfXJM+5r7rUvIrldfW0F1U+XY swi6-S192-274A mutations did not affect silencing at the centromere (imr::ura4), while swi6-S18-24A and S18- 117A mutants displayed decreases in silencing (Fig. 3C). ------- COMMENT: 9f1403044bcde8c5 30 2umfXJM+5r7rUvIrldfW0F1U+XY swi6-S192-274A mutations did not affect silencing at the centromere (imr::ura4), while swi6-S18-24A and S18- 117A mutants displayed decreases in silencing (Fig. 3C). ------- COMMENT: 9f1403044bcde8c5 31 paancIxiksiECg+G1Yy4bsEVftw In wild-type cells, both Clr3 and Mit1 were enriched on centromeric repeats; this localization was decreased in swi6D or chp2D cells, though substantial Mit1 was retained in swi6D cells, as reported previously (Sadaie et al. 2008). ------- COMMENT: 9f1403044bcde8c5 32 paancIxiksiECg+G1Yy4bsEVftw In wild-type cells, both Clr3 and Mit1 were enriched on centromeric repeats; this localization was decreased in swi6D or chp2D cells, though substantial Mit1 was retained in swi6D cells, as reported previously (Sadaie et al. 2008). ------- COMMENT: 9f1403044bcde8c5 33 paancIxiksiECg+G1Yy4bsEVftw In wild-type cells, both Clr3 and Mit1 were enriched on centromeric repeats; this localization was decreased in swi6D or chp2D cells, though substantial Mit1 was retained in swi6D cells, as reported previously (Sadaie et al. 2008). ------- COMMENT: 9f1403044bcde8c5 34 paancIxiksiECg+G1Yy4bsEVftw In wild-type cells, both Clr3 and Mit1 were enriched on centromeric repeats; this localization was decreased in swi6D or chp2D cells, though substantial Mit1 was retained in swi6D cells, as reported previously (Sadaie et al. 2008). ------- COMMENT: 9f1403044bcde8c5 35 paancIxiksiECg+G1Yy4bsEVftw In wild-type cells, both Clr3 and Mit1 were enriched on centromeric repeats; this localization was decreased in swi6D or chp2D cells, though substantial Mit1 was retained in swi6D cells, as reported previously (Sadaie et al. 2008). ------- COMMENT: 9f1403044bcde8c5 36 paancIxiksiECg+G1Yy4bsEVftw In wild-type cells, both Clr3 and Mit1 were enriched on centromeric repeats; this localization was decreased in swi6D or chp2D cells, though substantial Mit1 was retained in swi6D cells, as reported previously (Sadaie et al. 2008). ------- COMMENT: 9f1403044bcde8c5 37 sR8zGdkCQC9Udxef7lMVE9GV0VM Similarly, Clr3 and Mit1 localization was decreased in ckb1D and swi6-S18-117A mutants, al- though this decrease was less in swi6-S18-117A than in ckb1D cells. ------- COMMENT: 9f1403044bcde8c5 38 sR8zGdkCQC9Udxef7lMVE9GV0VM Similarly, Clr3 and Mit1 localization was decreased in ckb1D and swi6-S18-117A mutants, al- though this decrease was less in swi6-S18-117A than in ckb1D cells. ------- COMMENT: 9f1403044bcde8c5 39 sR8zGdkCQC9Udxef7lMVE9GV0VM Similarly, Clr3 and Mit1 localization was decreased in ckb1D and swi6-S18-117A mutants, al- though this decrease was less in swi6-S18-117A than in ckb1D cells. ------- COMMENT: 9f1403044bcde8c5 40 sR8zGdkCQC9Udxef7lMVE9GV0VM Similarly, Clr3 and Mit1 localization was decreased in ckb1D and swi6-S18-117A mutants, al- though this decrease was less in swi6-S18-117A than in ckb1D cells. ------- COMMENT: 9f25f26d17f2ec78 9 E6CgmvljMH2TSFllmLYY0HvfjkY zygotic meiosis random spore analysis, ------- COMMENT: 9f25f26d17f2ec78 10 xlWeIzs6M2Vz2fb/kKng37/oI2s (comment: azygotic meiotic cell cyle) ------- COMMENT: 9f25f26d17f2ec78 11 ARVKhee5O7YO+lfNU76CmyLndHc azygotic meiotic cell cycle/timing of pre-meiotic DNA replication is normal ------- COMMENT: 9f25f26d17f2ec78 12 E6CgmvljMH2TSFllmLYY0HvfjkY zygotic meiosis random spore analysis, ------- COMMENT: 9f25f26d17f2ec78 13 cnPY2VRFlJTmlWXXVnJQPRAtDQ0 zygotic meiosis random spore analysis ------- COMMENT: 9f25f26d17f2ec78 14 IVx7U1gBB/cJaW0j5ghvBjnDNac (comment: This phenotype is not seen when cells undergo azygotic meiosis) ------- COMMENT: 9f25f26d17f2ec78 15 3QMVAtNTpoISHs65ZyGcFsQ6Dqc presence of more than 2 SPBs dots after meiotic nuclear divisions 19.6% zygotes exhibit abnormal meiotic division during zygotic meiosis ------- COMMENT: 9f25f26d17f2ec78 19 cnPY2VRFlJTmlWXXVnJQPRAtDQ0 zygotic meiosis random spore analysis ------- COMMENT: 9f25f26d17f2ec78 20 47y3lODAwKTvtPtHVzg7CctKTx0 (comment: zygotic) ------- COMMENT: 9f25f26d17f2ec78 22 47y3lODAwKTvtPtHVzg7CctKTx0 (comment: zygotic) ------- COMMENT: 9f25f26d17f2ec78 24 cnPY2VRFlJTmlWXXVnJQPRAtDQ0 zygotic meiosis random spore analysis ------- COMMENT: 9f25f26d17f2ec78 26 AKO5uKYW7w0d2ZvjUWLeAGVgcAE zygotic random spore analysis ------- COMMENT: 9f25f26d17f2ec78 27 47y3lODAwKTvtPtHVzg7CctKTx0 (comment: zygotic) ------- COMMENT: 9f25f26d17f2ec78 28 47y3lODAwKTvtPtHVzg7CctKTx0 (comment: zygotic) ------- COMMENT: 9f25f26d17f2ec78 29 49QJpoA2u5fO4IU1USjJA7j1vyc zygotic / >80% of asci have 4 spores ------- COMMENT: 9f25f26d17f2ec78 30 OdqRksqoybPAdbI3Wu5DuATn6/U zygotic/ random spore analysis ------- COMMENT: 9f25f26d17f2ec78 31 gAxxW9JFFBVekXcAxflpVXBeyJY azygotic/ slight advance in the timing of MI and MII ------- COMMENT: 9f25f26d17f2ec78 32 QcaQDOZ18qAjSvbFZpFkkzXt1YU zygotic//presence of 4x fusion protein restores the ability of cig1 cig2 puc1 rem1 quadruple deletion strain to undergo pre meiotic DNA replication ------- COMMENT: 9f25f26d17f2ec78 33 q0aemHDaywr35XSFs4zVWD7wpEA azygotic// presence of 4x fusion protein restores the ability of cig1 cig2 puc1 rem1 quadruple deletion strain to undergo pre meiotic DNA replication ------- COMMENT: 9f25f26d17f2ec78 34 VKIeaUWBniSE94ll33JGFg/xu/w (comment: azygotic) ------- COMMENT: 9f25f26d17f2ec78 35 KouL+tIyxU5b/w6hDUYXM+wpN64 (comment: zygotic meiosis) ------- COMMENT: 9f25f26d17f2ec78 36 PHKPnxpiiWL5U12UBsLI+VZAdAs zygotic meiosis/ Random spore analysis ------- COMMENT: 9f25f26d17f2ec78 37 6EHiP0Vng8vGsb59V9U6qqS+d4Y azygotic meiosis, rem1 and crs1 do not have a major role in azygotic meiosis ------- COMMENT: 9f25f26d17f2ec78 38 47y3lODAwKTvtPtHVzg7CctKTx0 (comment: zygotic) ------- COMMENT: 9f25f26d17f2ec78 39 gAxxW9JFFBVekXcAxflpVXBeyJY azygotic/ slight advance in the timing of MI and MII ------- COMMENT: 9f25f26d17f2ec78 40 47y3lODAwKTvtPtHVzg7CctKTx0 (comment: zygotic) ------- COMMENT: 9f25f26d17f2ec78 41 47y3lODAwKTvtPtHVzg7CctKTx0 (comment: zygotic) ------- COMMENT: 9f25f26d17f2ec78 42 47y3lODAwKTvtPtHVzg7CctKTx0 (comment: zygotic) ------- COMMENT: 9f25f26d17f2ec78 43 47y3lODAwKTvtPtHVzg7CctKTx0 (comment: zygotic) ------- COMMENT: 9f25f26d17f2ec78 44 47y3lODAwKTvtPtHVzg7CctKTx0 (comment: zygotic) ------- COMMENT: 9f25f26d17f2ec78 45 47y3lODAwKTvtPtHVzg7CctKTx0 (comment: zygotic) ------- COMMENT: 9f729989228125e4 6 D+hey0afH4HJFl0VtQKzi67EOnA The rap1∆ trt1∆ strain with circular chromosomes and lacking telomere DNA showed no accelera- tion in replication timing compared to the control trt1∆ strain, ------- COMMENT: 9f729989228125e4 8 R0ybwCfuPvIoUaX4v9l28IsZc4Y Fig. S2 ------- COMMENT: 9f729989228125e4 9 R0ybwCfuPvIoUaX4v9l28IsZc4Y Fig. S2 ------- COMMENT: 9f729989228125e4 10 R0ybwCfuPvIoUaX4v9l28IsZc4Y Fig. S2 ------- COMMENT: 9f729989228125e4 11 R0ybwCfuPvIoUaX4v9l28IsZc4Y Fig. S2 ------- COMMENT: 9f729989228125e4 15 glJW1aOyj4jhjS/C9sk9B+zU/dU Fig. 4a ------- COMMENT: 9f729989228125e4 19 rDR41po8gfpi5g9cNpYWWk5easQ Fig. 5 ------- COMMENT: 9f729989228125e4 20 rDR41po8gfpi5g9cNpYWWk5easQ Fig. 5 ------- COMMENT: 9f89b5e1df7ace40 1 b2OP+ZvYUKXzYlQbzTV4yYdbU1g fig 1 The data sug- gest that the mutants are not deficient in termination effi- ciency. ------- COMMENT: 9f89b5e1df7ace40 2 KP1VI/gcVDvTADfswyvYmP+/M6s Mutated Rpc11p subunits associate with Pol III and impair its RNA 3􏰌 cleavage activity. ------- COMMENT: 9f89b5e1df7ace40 5 b2OP+ZvYUKXzYlQbzTV4yYdbU1g fig 1 The data sug- gest that the mutants are not deficient in termination effi- ciency. ------- COMMENT: 9f89b5e1df7ace40 6 b2OP+ZvYUKXzYlQbzTV4yYdbU1g fig 1 The data sug- gest that the mutants are not deficient in termination effi- ciency. ------- COMMENT: 9f89b5e1df7ace40 9 gX8+TEIqZcDwT9bJijXHzSMxzis (comment: trna chaperone) ------- COMMENT: 9fbdcdc0e32c2c2c 1 hzKs3EwIAZ5kR/wlIBGmUDcmTGA multinucleate inferred from DNA content ------- COMMENT: 9fbdcdc0e32c2c2c 4 hzKs3EwIAZ5kR/wlIBGmUDcmTGA multinucleate inferred from DNA content ------- COMMENT: 9fbdcdc0e32c2c2c 13 hzKs3EwIAZ5kR/wlIBGmUDcmTGA multinucleate inferred from DNA content ------- COMMENT: 9fbdcdc0e32c2c2c 22 hzKs3EwIAZ5kR/wlIBGmUDcmTGA multinucleate inferred from DNA content ------- COMMENT: 9fbdcdc0e32c2c2c 23 hzKs3EwIAZ5kR/wlIBGmUDcmTGA multinucleate inferred from DNA content ------- COMMENT: 9fbdcdc0e32c2c2c 27 UgWpsw9EtT5muNLfGEaaXupdNKw same as spt20delta alone ------- COMMENT: 9fbdcdc0e32c2c2c 28 UgWpsw9EtT5muNLfGEaaXupdNKw same as spt20delta alone ------- COMMENT: 9fbdcdc0e32c2c2c 29 UgWpsw9EtT5muNLfGEaaXupdNKw same as spt20delta alone ------- COMMENT: 9fbdcdc0e32c2c2c 52 igXq3WWOr1iIQF5+MLB7PHM2X0c (comment: CHECK punctate) ------- COMMENT: a02efb01ac764f2e 84 2ngHVES5cT2J6g856KqY6zVZ3hI conditions under which pat1-114 alone induces meiosis & sporulation ------- COMMENT: a03f8c91ccf38c01 26 U5nmlofP95W0H1PkH2mLkZ9akjs fig 2G ------- COMMENT: a03f8c91ccf38c01 27 U5nmlofP95W0H1PkH2mLkZ9akjs fig 2G ------- COMMENT: a03f8c91ccf38c01 28 U5nmlofP95W0H1PkH2mLkZ9akjs fig 2G ------- COMMENT: a03f8c91ccf38c01 29 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: a03f8c91ccf38c01 30 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: a03f8c91ccf38c01 31 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: a03f8c91ccf38c01 32 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: a03f8c91ccf38c01 33 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: a03f8c91ccf38c01 36 ILeF24xBgYJHr7h036KuTVLPgBo fig 5 (measured at 4 um spindle. WT has 6%) ------- COMMENT: a03f8c91ccf38c01 37 aMY425eWti6R4WLdB/FEzEmzVqk Fig. 5B and D ------- COMMENT: a03f8c91ccf38c01 38 uCi/2FKciHz2j1KRzqP7u0u1+AM increased duration of metaphase Fig. 5E ------- COMMENT: a03f8c91ccf38c01 39 FZQVLLvybg1r2PzJ0EfTWb3LWh0 fig 5f ------- COMMENT: a03f8c91ccf38c01 40 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: a03f8c91ccf38c01 41 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: a059f85434b62eb8 3 pwiwp5F6d0/rGXJxW/JMmlh2yGE Microscopy co-localization ------- COMMENT: a07f7b8bc158c1e0 3 rqJFPUO9Wxrstv6ZBK0Ix/0/+OE in vitro assay for activity, phenotype for process ------- COMMENT: a07f7b8bc158c1e0 4 B3qgmlNznrzD7D+TXoSTLTPKRtM Figure 3A during spindle checkpoint ------- COMMENT: a07f7b8bc158c1e0 11 W8U2HOh5c9FHrXYcx0iyvAr6hdQ Figure 7A ------- COMMENT: a07f7b8bc158c1e0 16 YeSKXTuBNlcZ1BPbB4mqa07PHuU figure 7A ------- COMMENT: a07f7b8bc158c1e0 17 YeSKXTuBNlcZ1BPbB4mqa07PHuU figure 7A ------- COMMENT: a07f7b8bc158c1e0 18 YeSKXTuBNlcZ1BPbB4mqa07PHuU figure 7A ------- COMMENT: a07f7b8bc158c1e0 22 wf185u4DRDkg7Sbpsn3gLe6+2NY Figure 4E ------- COMMENT: a07f7b8bc158c1e0 24 1U5lP9ZgJub272Ft6ZiWZgVRrlw In zygotes undergoing meiotic divisions, WT Bub1±GFPp became associated with the centromeres after meiotic prophase and remained associated until anaphase I, con®rming previous reports using ®xed cells (Bernard et al., 2001; Figure 7B, WT, f±h). ------- COMMENT: a07f7b8bc158c1e0 25 1U5lP9ZgJub272Ft6ZiWZgVRrlw In zygotes undergoing meiotic divisions, WT Bub1±GFPp became associated with the centromeres after meiotic prophase and remained associated until anaphase I, con®rming previous reports using ®xed cells (Bernard et al., 2001; Figure 7B, WT, f±h). ------- COMMENT: a07f7b8bc158c1e0 26 QL4xLcCxm6uRgsW87Av5uehYmAA figure 7B ------- COMMENT: a07f7b8bc158c1e0 27 QL4xLcCxm6uRgsW87Av5uehYmAA figure 7B ------- COMMENT: a07f7b8bc158c1e0 28 /lBu8hCtk+JMcFZ436vl7DRdmj0 Figure 7Ca ------- COMMENT: a07f7b8bc158c1e0 29 tg+8xWJI45ikBShzAqRa+96Eapk decreased rate of spindle phase I elongation (70 mins. vs 40 wt) ------- COMMENT: a07f7b8bc158c1e0 30 dz5PbkAlKdjsc5IZYj96z2cNx18 normal rate of spindle phase I elongation. MI spindle elongation time is reduced to 40 min in rec7-146 bub1D cells (Figure 8Ad, upper panel) ------- COMMENT: a09af17a2956146d 1 xt6pE06e3dmBmxAYxJ3L8y9Ajb0 partial derepression of marker gene at silent mating-type cassette; measured by cell growth spot assay ------- COMMENT: a09af17a2956146d 2 PBjLmEYjWekHeOdSDlr291+SHHk measured by cell growth spot assay ------- COMMENT: a09af17a2956146d 3 03ienHN103hzbhokIapL7vIAbk8 spot assay ------- COMMENT: a09af17a2956146d 4 PBjLmEYjWekHeOdSDlr291+SHHk measured by cell growth spot assay ------- COMMENT: a09af17a2956146d 5 PBjLmEYjWekHeOdSDlr291+SHHk measured by cell growth spot assay ------- COMMENT: a09af17a2956146d 30 i2lyHvH8fxKgf0dzC3ElB5BsYUw colocalizes with H3K9me2 ------- COMMENT: a09af17a2956146d 31 i2lyHvH8fxKgf0dzC3ElB5BsYUw colocalizes with H3K9me2 ------- COMMENT: a09af17a2956146d 32 i2lyHvH8fxKgf0dzC3ElB5BsYUw colocalizes with H3K9me2 ------- COMMENT: a0c8d1e63698e9f9 1 LTCc6QxUmhKkdYinRocxWnxrUac structure ------- COMMENT: a0db0af7ec94b4a9 17 47ieiKqN2qlN40xtUtZrbUvdmY0 (comment: tetrads only) ------- COMMENT: a0eb5775b3ce7599 3 ezBplEN2zFCDKyPuiuWLTiDhI5Y (comment: (vw: changed from nuclear lumen to nuclear periphery)) ------- COMMENT: a0f6f6a0c5ab5507 1 GIix5668G6m/1tCgCIYkzNaf7aU (comment: through conserved cysteines) ------- COMMENT: a11f462edea7ec81 6 fusLTTvnwRKkmznIKBnxLbEdZiQ (comment: CHECK during mitotic M phase) ------- COMMENT: a11f462edea7ec81 15 fusLTTvnwRKkmznIKBnxLbEdZiQ (comment: CHECK during mitotic M phase) ------- COMMENT: a11f462edea7ec81 16 fusLTTvnwRKkmznIKBnxLbEdZiQ (comment: CHECK during mitotic M phase) ------- COMMENT: a11f462edea7ec81 63 2kntFv+JmXko+I+TbyD43GFlnR4 (comment: non additive) ------- COMMENT: a11f462edea7ec81 85 Gp2Yls0E0vrmCUqrxdNKiLBEyLk (comment: I think the pkl rigor is spb tethered here?) ------- COMMENT: a11f462edea7ec81 93 Nxts7V0BYse5/+KwGg6F7CiCsPI (comment: I want to represent the microtubule based-transporter function and cargo) ------- COMMENT: a11f462edea7ec81 94 Nxts7V0BYse5/+KwGg6F7CiCsPI (comment: I want to represent the microtubule based-transporter function and cargo) ------- COMMENT: a11f462edea7ec81 97 sreBbjl2gDaomxCok/tn8tXFMjw Fig. 5B and Videos 1–4 ------- COMMENT: a11f462edea7ec81 98 sreBbjl2gDaomxCok/tn8tXFMjw Fig. 5B and Videos 1–4 ------- COMMENT: a11f462edea7ec81 99 sreBbjl2gDaomxCok/tn8tXFMjw Fig. 5B and Videos 1–4 ------- COMMENT: a11f462edea7ec81 102 sreBbjl2gDaomxCok/tn8tXFMjw Fig. 5B and Videos 1–4 ------- COMMENT: a17bd0ab5d302ba7 1 9nWcaClICOgQQHAthS9f66+wgVU (comment: hypermutator) ------- COMMENT: a17bd0ab5d302ba7 4 4WkoqjqH1Dvfno4NiPZ7dJ+f7Nc (comment: CHECK cdc22-D57N) ------- COMMENT: a17bd0ab5d302ba7 10 sEeVy4dUT7IjmFcgoHEONnKMajc (comment: CHECK Chk1 is partially phosphorylated) ------- COMMENT: a17bd0ab5d302ba7 11 dip7/1JJJPkS0b5QBs70WWyx9to (comment: CHECK Cds1 is partially phosphorylated) ------- COMMENT: a17bd0ab5d302ba7 12 01XnE2NhDnLDLGTr4eBoWlnMpb4 pfh1 repression by thiamine ------- COMMENT: a17bd0ab5d302ba7 13 YxBiY0akvwxH9CHmjlQrDwgwI54 overexpression of Pfh1 suppresses growth defects at 30˚ C in minimal medium and the sensitivity to MMS ------- COMMENT: a17bd0ab5d302ba7 15 cDCcu4b9DLGWUJnay9lmnPOM+k4 reduced mutation rate ------- COMMENT: a17bd0ab5d302ba7 16 cDCcu4b9DLGWUJnay9lmnPOM+k4 reduced mutation rate ------- COMMENT: a17bd0ab5d302ba7 28 GjSltmwWmoK8R14n4+WxYjssvkQ (comment: equivalent substitution to cdc20-P287R) ------- COMMENT: a17bd0ab5d302ba7 29 +1PyLfrjIVL8knr6ekLHGFWo3dU double mutants are frequently slow growing ------- COMMENT: a17bd0ab5d302ba7 30 +1PyLfrjIVL8knr6ekLHGFWo3dU double mutants are frequently slow growing ------- COMMENT: a17bd0ab5d302ba7 34 aUGMASF73A9GDLF4TDy6pYJrne4 DNA combing ------- COMMENT: a19f8cb91e1b57a2 3 3EfvjaMCp77T+8C8sptTG84+fIc Fig1B ------- COMMENT: a19f8cb91e1b57a2 5 bbL88bVVvfd44kn4qk0riac2hgA Fig1B normal interphase MTs required to establish early orientation of mitotic spindle by aligning SPBs with long axis of cell ------- COMMENT: a19f8cb91e1b57a2 6 ULvfvAJ3D6sihAg7IIt78oBY0hE Fig1B and 2B,C normal MTs required to establish early orientation of mitotic spindle by aligning SPBs with long axis of cell ------- COMMENT: a19f8cb91e1b57a2 11 psJbSkNdsDHlhumDf18+Zf6KWvI Fig3B ------- COMMENT: a19f8cb91e1b57a2 12 SIQcQi6JpMR+PRA1HramfjG+5Sw Fig3B, C ------- COMMENT: a19f8cb91e1b57a2 13 3LXC8TEEqa+JID7DDQJADVBmKtc Fig3B, C As expected, the range of SPB trajectory angles was much wider than in wild-type cells (Fig. 2C, Fig. 3B,C ------- COMMENT: a19f8cb91e1b57a2 15 psJbSkNdsDHlhumDf18+Zf6KWvI Fig3B ------- COMMENT: a19f8cb91e1b57a2 18 wj2VSE893AMBlEWR8+9Ey55om+8 Fig 2B ------- COMMENT: a19f8cb91e1b57a2 26 6s3Jap7liSgVy81i8YRu2QU4hjs Fig 2A-C This oscillatory movement was not perturbed by Latrunculin A treatment, but was lost in cells treated with MBC or in mto1Δ cells, and was reduced in tip1Δ cells ------- COMMENT: a1aa202992e46314 1 pX+f/flJj6s1BA676WLV5FPGhBk MIPC was not detected, IPC had accumulated and one unidentified sphingolipid spot was observed (Fig. 2A). These results indicate that all three imt genes are required for MIPCsynthesis and that MIPC was not produced in imt1imt2imt3 disruptant mutants. ------- COMMENT: a1aa202992e46314 2 AouUohPub3lo/oVIprJPM5nBADA MIPC was not detected, IPC had accumulated and one unidentified sphingolipid spot was observed (Fig. 2A). These results indicate that all three imt genes are required for MIPC synthesis and that MIPC was not produced in imt1imt2imt3 disruptant mutants. ------- COMMENT: a1aa202992e46314 3 AouUohPub3lo/oVIprJPM5nBADA MIPC was not detected, IPC had accumulated and one unidentified sphingolipid spot was observed (Fig. 2A). These results indicate that all three imt genes are required for MIPC synthesis and that MIPC was not produced in imt1imt2imt3 disruptant mutants. ------- COMMENT: a1aa202992e46314 4 AouUohPub3lo/oVIprJPM5nBADA MIPC was not detected, IPC had accumulated and one unidentified sphingolipid spot was observed (Fig. 2A). These results indicate that all three imt genes are required for MIPC synthesis and that MIPC was not produced in imt1imt2imt3 disruptant mutants. ------- COMMENT: a1aa202992e46314 5 v9fJE72c4TClTWziDVZscdRk21c Interestingly, a significant amount of IPC accumulated and MIPC decreased in sur2 cells. The scs7 mutation also caused a reduction in MIPC content. These results suggest that Imt proteins exhibit weak activity towards dihydroceramide-containing IPC, such that a reduction in MIPC was observed in sur2 and scs7 mutants. ------- COMMENT: a1aa202992e46314 6 v9fJE72c4TClTWziDVZscdRk21c Interestingly, a significant amount of IPC accumulated and MIPC decreased in sur2 cells. The scs7 mutation also caused a reduction in MIPC content. These results suggest that Imt proteins exhibit weak activity towards dihydroceramide-containing IPC, such that a reduction in MIPC was observed in sur2 and scs7 mutants. ------- COMMENT: a1aa202992e46314 7 v9fJE72c4TClTWziDVZscdRk21c Interestingly, a significant amount of IPC accumulated and MIPC decreased in sur2 cells. The scs7 mutation also caused a reduction in MIPC content. These results suggest that Imt proteins exhibit weak activity towards dihydroceramide-containing IPC, such that a reduction in MIPC was observed in sur2 and scs7 mutants. ------- COMMENT: a1aa202992e46314 8 v9fJE72c4TClTWziDVZscdRk21c Interestingly, a significant amount of IPC accumulated and MIPC decreased in sur2 cells. The scs7 mutation also caused a reduction in MIPC content. These results suggest that Imt proteins exhibit weak activity towards dihydroceramide-containing IPC, such that a reduction in MIPC was observed in sur2 and scs7 mutants. ------- COMMENT: a1aa202992e46314 9 m1qTHAApOhvpqcquCSBFToOt7u0 These results indicate that all three Imt-GFP fusion proteins primarily localize to the Golgi and trans-Golgi network, similar to S. cerevisiae Sur1p (Lisman et al., 2004). ------- COMMENT: a1aa202992e46314 10 m1qTHAApOhvpqcquCSBFToOt7u0 These results indicate that all three Imt-GFP fusion proteins primarily localize to the Golgi and trans-Golgi network, similar to S. cerevisiae Sur1p (Lisman et al., 2004). ------- COMMENT: a1aa202992e46314 11 m1qTHAApOhvpqcquCSBFToOt7u0 These results indicate that all three Imt-GFP fusion proteins primarily localize to the Golgi and trans-Golgi network, similar to S. cerevisiae Sur1p (Lisman et al., 2004). ------- COMMENT: a1aa202992e46314 12 DPwcdbV3XuC2B+/A/FJB0A+hXwk Single and double disruptants had a normal cell shape, but the imt1imt2imt3 disruptants were round or pear shaped under normal growth conditions (Fig. 4). (comment: note that these do not look particularly rounded or pear-shaped and fit our "stubby" morphology better) ------- COMMENT: a1aa202992e46314 13 enTpHRliOm7hKqDek3e8xgNFMRs Sensitivity to Ca2+ was determined by a visual spot assay on YES medium (Fig. 5). Single imt mutants did not exhibit obvious Ca2+ sensitivity, whereas the imt1imt2imt3 mutant did. ------- COMMENT: a1aa202992e46314 14 AmLwbHcUyUTKnr9iWJK41ucI/5A imt1imt2imt3 cells were also found to be sensitive to 3 g/ml nystatin and to 0.5 g/ml amphotericin B (Fig. 5). ------- COMMENT: a1aa202992e46314 15 AmLwbHcUyUTKnr9iWJK41ucI/5A imt1imt2imt3 cells were also found to be sensitive to 3 g/ml nystatin and to 0.5 g/ml amphotericin B (Fig. 5). ------- COMMENT: a1aa202992e46314 16 19kf1VWq6oCys5vLEuLPajDozGQ By contrast, imt1imt2imt3 cells had slightly smaller vacuoles compared with those of wild- type cells under normal conditions (Fig. 6) ------- COMMENT: a1aa202992e46314 17 19kf1VWq6oCys5vLEuLPajDozGQ By contrast, imt1imt2imt3 cells had slightly smaller vacuoles compared with those of wild- type cells under normal conditions (Fig. 6) ------- COMMENT: a1aa202992e46314 18 T05MtgpzaD4brGn/FICD4DSR4bE Whereas imt1imt2imt3 cells exhibited enhanced levels of fluorescence, sterols were detected throughout the plasma membrane (Fig. 7). These results suggest that aberrant localization or enrichment of sterols in the plasma membrane caused sensitivity to polyene antibiotics in MIPC-deficient cells. ------- COMMENT: a1aa202992e46314 19 AcRzAr6VIUWUQUEeJlwReBw+Z7M Expressed in wild-type S. pombe grown in rich medium, Aat1-GFP exhibited punctate fluorescence, suggesting that Aat1-GFP localized to the Golgi apparatus (Fig. 8A,B). When the cells were shifted to a nitrogen- free medium, Aat1-GFP was transported from the Golgi apparatus to the plasma membrane within 30 minutes, followed by endocytosis and transport to the vacuolar lumen. ------- COMMENT: a1aa202992e46314 20 rBtGE1zQXZIHM9MoXdqrFozlls8 Interestingly, endocytosis of Aat1-GFP was severely impaired and Aat1-GFP remained at the plasma membrane after 5 hours of incubation (Fig. 8A). Thus, we conclude that the MIPC- deficient mutant is impaired in its ability to internalize plasma- membrane proteins to the vacuole. ------- COMMENT: a1aa202992e46314 21 rBtGE1zQXZIHM9MoXdqrFozlls8 Interestingly, endocytosis of Aat1-GFP was severely impaired and Aat1-GFP remained at the plasma membrane after 5 hours of incubation (Fig. 8A). Thus, we conclude that the MIPC- deficient mutant is impaired in its ability to internalize plasma- membrane proteins to the vacuole. ------- COMMENT: a206afb9651e2886 31 KXQDzB8BhqRRf/7yNJqJYrAzxHI We conclude that Mid1p recruits Rng2p to cortical nodes at the division site and that Rng2p, in turn, recruits other components of the actomyosin ring to cortical nodes, thereby ensuring correct placement of the division site. ------- COMMENT: a21807569990efc2 1 FnI8kzviS6kGF4O7mHuCSZyPdg4 Figure 3c ------- COMMENT: a21807569990efc2 4 LQmuKZbL9ND56pzzWctDT58tWRE Figure 3C ------- COMMENT: a21807569990efc2 6 ixRkOLFohv02Tg0l7oDklz6nMyQ Figure 4b ------- COMMENT: a21807569990efc2 7 +9U0KAAWhidPxfr8K6AizO375Zg Figure S2B ------- COMMENT: a21807569990efc2 15 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: a21807569990efc2 17 FnI8kzviS6kGF4O7mHuCSZyPdg4 Figure 3c ------- COMMENT: a21807569990efc2 19 NjPx0oyNCLmXXf+Ikj3kTqyGIEU Figure 3d ------- COMMENT: a21807569990efc2 20 bBKa+cOSgxLimLqWZYMQioRzSdA Figure 4c ------- COMMENT: a21807569990efc2 22 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: a21807569990efc2 23 KegEtMf0f7ynQlwOoepubhFA9CI Figure 5D These observations indicate that the mip1 mutation does not affect the TORC1-dependent phosphorylation of Sck1, Sck2 and Maf1. ------- COMMENT: a21807569990efc2 26 0JtWWbGerTmkzK8lAu6rlAYe7tU Figure 5E ------- COMMENT: a21807569990efc2 28 D4YOkgqUkb74Inu/nWmTeYeP9CQ Fig. 1A, Fig. S1A ------- COMMENT: a21807569990efc2 33 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: a21807569990efc2 39 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: a21807569990efc2 45 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: a21807569990efc2 48 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: a21807569990efc2 49 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: a21807569990efc2 50 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: a21807569990efc2 56 zKaOHTxo+hQor7o/KoPoFH5sAGc Thus, in addition to Psk1, Atg13 also appears to be phosphorylated by TORC1 in a manner dependent on Mip1 Tyr-533, a residue critical for the TOS motif-mediated recruitment of TORC1 substrates. ------- COMMENT: a2468b284d810cf8 1 hOuXb+VHjFOEPsLaiFuHHF7gdso (Figure 1A) In a mob1-R4 byr4::ura4􏰀 mutant, and also in cdc16-116 grown for 5 hr at 36􏰁C, cdc11p accumulated in the hyperphosphorylated (3) form ------- COMMENT: a2468b284d810cf8 2 c1QJ/p/6BJbtXZKe+lxqTv/85hc (Figure 1A) In a mob1-R4 byr4::ura4􏰀 mutant, and also in cdc16-116 grown for 5 hr at 36􏰁C, cdc11p accumulated in the hyperphosphorylated (3) form. Later: in mutants such as cdc16-116 or byr4::ura4􏰀, in which cdc7p is present on both spindle pole bodies, the hyperphosphorylated form (3) of cdc11p is more abundant. ------- COMMENT: a2468b284d810cf8 3 /yElxHxKI2kvnsOe0zcBYV8QsSQ (Figure 1B) n G2-arrested cdc2-17 cells overexpressing spg1p, cdc11p was predominantly in the hyperphosphorylated form ------- COMMENT: a2468b284d810cf8 4 br3w6gmvHLENnDyOSNpY3aNnp4U (comment: CHECK HYPERPHOSPHORYLATD FORM) Together, these data demonstrate, first, that mitotic cdc2p activity is not required for hyperphosphorylation of cdc11p and, second, that activation of the SIN correlates with accu- mulation of hyperphosphorylated cdc11p. ------- COMMENT: a2468b284d810cf8 5 7ghF7TKXHSy5POP0myHogOD9Alg (comment: CHECK HYPERPHOSPHORYLATION) (Figure 1C) In the mutant plo1-ts4, which is defective in SIN signaling but not spindle formation [10], hyperphosphorylated cdc11p was observed during mi- tosis, even though the cells were not septating . ------- COMMENT: a2468b284d810cf8 6 ojGSPFJ8KWbo2iRKGWpxJZclCLE (Figure 1C, and data not shown) Immunofluorescence indicated that both byr4p and cdc7p showed a normal, asymmetric distribution during mitosis ------- COMMENT: a2468b284d810cf8 7 ojGSPFJ8KWbo2iRKGWpxJZclCLE (Figure 1C, and data not shown) Immunofluorescence indicated that both byr4p and cdc7p showed a normal, asymmetric distribution during mitosis ------- COMMENT: a2468b284d810cf8 8 CNEpmpq6zY92cmttiLQIACzwG70 (see the Supplementary) Additional experiments indicated that neither mph1p nor fin1p regulate the phosphorylation of cdc11p ------- COMMENT: a2468b284d810cf8 9 CNEpmpq6zY92cmttiLQIACzwG70 (see the Supplementary) Additional experiments indicated that neither mph1p nor fin1p regulate the phosphorylation of cdc11p ------- COMMENT: a2468b284d810cf8 10 CNEpmpq6zY92cmttiLQIACzwG70 (see the Supplementary) Additional experiments indicated that neither mph1p nor fin1p regulate the phosphorylation of cdc11p ------- COMMENT: a2468b284d810cf8 11 TcNtc60dFPuz1yHHQeGonGrD2BM (Figure 1D) The hyperphosphorylated form (3) of cdc11p was observed during mitosis in both sid2-250 and sid1- 239 mutants ------- COMMENT: a2468b284d810cf8 12 TcNtc60dFPuz1yHHQeGonGrD2BM (Figure 1D) The hyperphosphorylated form (3) of cdc11p was observed during mitosis in both sid2-250 and sid1- 239 mutants ------- COMMENT: a2468b284d810cf8 13 TcNtc60dFPuz1yHHQeGonGrD2BM (Figure 1D) The hyperphosphorylated form (3) of cdc11p was observed during mitosis in both sid2-250 and sid1- 239 mutants ------- COMMENT: a2468b284d810cf8 14 sqcAIg5KkdWZoghKhY8aD1MsvyI (Figure 1E) In contrast, in cdc7-24, the hyperphosphorylated form of cdc11p (3) was greatly reduced, and the intensity of the hypophos- phorylated forms (1 and 2) increased ------- COMMENT: a2468b284d810cf8 15 5Xcdtag7XKhUDJ4tzy9kogdBQYQ (comment: DNS) ------- COMMENT: a2468b284d810cf8 16 +5MjNHjzZ2mSV5vt6a1N18VH+ek (Figure 1G) No significant hyperphosphorylation of cdc11p occurred at 36􏰁C. ------- COMMENT: a2468b284d810cf8 17 +rW2X0ugbgFJB4MvYDKht+lE4hY In contrast, in cdc7-24, the hyperphosphorylated form of cdc11p (3) was greatly reduced, and the intensity of the hypophos- phorylated forms (1 and 2) increased (Figure 1E). A simi- lar result was observed in cdc7-A20 (data not shown). Furthermore, no mitotic hyperphosphorylation of cdc11p was seen in spg1-B8 at the nonpermissive temperature (Figure 1E), when cdc7p does not localize to the SPB [13]. Finally, when cdc7 was expressed ectopically in G2-arrested cells, cdc11p accumulated in the hyper- phosphorylated form (3) (Figure 1F). ------- COMMENT: a2468b284d810cf8 20 EbadZaRKET3cTMNWOm2RxVGjFT8 (Figure 1G) No significant hyperphosphorylation of cdc11p occurred at 36􏰁C . ------- COMMENT: a2468b284d810cf8 22 VtDKzj3DhzuMOHRZjhDGUO4QTTI (Figure 2B). Immunofluorescence showed that byr4p was also present on the SPB in the arrested cells ------- COMMENT: a2468b284d810cf8 25 no/vcsdP/bCtEGR9unMsDqvrPoI Less than 10% of these cells had cdc7p on the spindle pole body, consistent with previous studies [25]. ------- COMMENT: a2468b284d810cf8 28 WvkTlCCd6RPYuH6HSLiCMFJ3UPg (Figure 3B). However, at 36􏰁C, when cdc11p is no longer associated with the SPB [3, 4], most of the cdc11p was hypophosphory- lated (form 1) ------- COMMENT: a265654194a9e17a 9 uP2b450kx3y/u6z3D7bDUa+eEuM fig8 ------- COMMENT: a265654194a9e17a 10 uP2b450kx3y/u6z3D7bDUa+eEuM fig8 ------- COMMENT: a265654194a9e17a 11 uP2b450kx3y/u6z3D7bDUa+eEuM fig8 ------- COMMENT: a265654194a9e17a 12 uP2b450kx3y/u6z3D7bDUa+eEuM fig8 ------- COMMENT: a285637adcbb8409 18 4VfYO4Uxdqxx4nA27ykZpmSYxxE (comment: 30% at 120 min. (archery bow)) ------- COMMENT: a285637adcbb8409 19 odvl346FIHrJFN7+pqESow7UNYQ (comment: 85% at 160 min) ------- COMMENT: a285637adcbb8409 22 a1dM/ys/9G8EDQ9Dfhxcm3CjECg Figure 3A ------- COMMENT: a285637adcbb8409 23 a1dM/ys/9G8EDQ9Dfhxcm3CjECg Figure 3A ------- COMMENT: a285637adcbb8409 24 a1dM/ys/9G8EDQ9Dfhxcm3CjECg Figure 3A ------- COMMENT: a28d9f4e4dcc8ef3 53 B85ugDIk/64vaPNJ4WqzSLw/94c more sensitive than either single mutant ------- COMMENT: a28d9f4e4dcc8ef3 54 B85ugDIk/64vaPNJ4WqzSLw/94c more sensitive than either single mutant ------- COMMENT: a28d9f4e4dcc8ef3 55 B85ugDIk/64vaPNJ4WqzSLw/94c more sensitive than either single mutant ------- COMMENT: a28d9f4e4dcc8ef3 56 B85ugDIk/64vaPNJ4WqzSLw/94c more sensitive than either single mutant ------- COMMENT: a2c14f18afef292a 30 34fkuDSh/wTrg+nydpNeIzlBEcM anti-alpha-tubulin antibody used; included both pombe alpha-tubulin gene names in extension ------- COMMENT: a2ee596ebb2b3e9a 5 Dx0qqvhp3Ladj/K4GdaNvLdbjMU fig 6E ------- COMMENT: a2ee596ebb2b3e9a 6 nEaa8YKAJQrgM8JMf+0co+MNKbo Fig. 6E ------- COMMENT: a2ee596ebb2b3e9a 7 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: a2ee596ebb2b3e9a 9 qW0fooJwQWtfFzpmyf80rEliP/A fig 5c ------- COMMENT: a2ee596ebb2b3e9a 10 hQCQL3NZ4HqU46mI91XPhZQAeNk (comment: vw: changed to increased activation, and D333A allele) ------- COMMENT: a2ee596ebb2b3e9a 11 KjKmuhh6GKxt1Fo+IS8JFmXslFY (comment: CHECK asp1 365-920 overexpression only) ------- COMMENT: a2ee596ebb2b3e9a 12 KjKmuhh6GKxt1Fo+IS8JFmXslFY (comment: CHECK asp1 365-920 overexpression only) ------- COMMENT: a2ee596ebb2b3e9a 13 KjKmuhh6GKxt1Fo+IS8JFmXslFY (comment: CHECK asp1 365-920 overexpression only) ------- COMMENT: a2ee596ebb2b3e9a 14 FBUfDj7DevXRZGhxgziALtQsL0g (comment: CHECK asp1D333A allele only) ------- COMMENT: a2ee596ebb2b3e9a 15 FBUfDj7DevXRZGhxgziALtQsL0g (comment: CHECK asp1D333A allele only) ------- COMMENT: a2ee596ebb2b3e9a 16 5DWqo03puFeQtodWqcCbmJ6nfyM fig 1A ------- COMMENT: a2ee596ebb2b3e9a 17 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: a2ee596ebb2b3e9a 18 8cV7yovnTEPUG/+J1/TcJFLCfiw fig4E ------- COMMENT: a2ee596ebb2b3e9a 19 qW0fooJwQWtfFzpmyf80rEliP/A fig 5c ------- COMMENT: a2ee596ebb2b3e9a 20 uua1Qia/6wWRlPmDhEjrHoaiAuE fig6, fig 7 ------- COMMENT: a2ee596ebb2b3e9a 21 6sE0z6pXK73moUzXLeTpJRG0Nxg fig6A ------- COMMENT: a2ee596ebb2b3e9a 22 Dx0qqvhp3Ladj/K4GdaNvLdbjMU fig 6E ------- COMMENT: a2ee596ebb2b3e9a 23 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: a2ee596ebb2b3e9a 24 2SkyLksWV7akLwZiR4xvvnTu/2Q (comment: CHECK Fig.) ------- COMMENT: a321fd3e3c26eb98 1 jVL2SI6gvVCmQY4n6M/I8BuWB4c Neither the pol I/Rrn3p (Fig. 3, lane 3) nor the SpRrn7h complex fraction (Fig. 3, lanes 1 and 2) support accurate initiation of the S. pombe rRNA template, 30 D 131, on their own. However, when both are present, accurate transcriptional initiation of the S. pombe rDNA gene promoter is reconstituted (Fig. 3, lanes 4 and 5). ------- COMMENT: a321fd3e3c26eb98 2 jVL2SI6gvVCmQY4n6M/I8BuWB4c Neither the pol I/Rrn3p (Fig. 3, lane 3) nor the SpRrn7h complex fraction (Fig. 3, lanes 1 and 2) support accurate initiation of the S. pombe rRNA template, 30 D 131, on their own. However, when both are present, accurate transcriptional initiation of the S. pombe rDNA gene promoter is reconstituted (Fig. 3, lanes 4 and 5). ------- COMMENT: a321fd3e3c26eb98 3 UvbxxU4Piya/A8T17qzDQjEpdA8 3.4. The putative SpRrn11h co-fractionates with Sp-MHRrn7h and rDNA transcription initiation activity ------- COMMENT: a321fd3e3c26eb98 4 XXGFj0NWy1EWvQiVV7indJudrRc 3.7. Association of SpRrn7h with rDNA core promoter sequences ------- COMMENT: a387442f1547e80a 4 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a387442f1547e80a 7 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a387442f1547e80a 9 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a387442f1547e80a 24 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a39841010d3ec10f 1 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: a39841010d3ec10f 2 wmvcPI6EhbLjepW8SN1tY/jbppk Figure 1A inferred from increased duration of mitosis ------- COMMENT: a39841010d3ec10f 3 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: a39841010d3ec10f 4 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: a39841010d3ec10f 8 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: a39841010d3ec10f 9 kZVrPEANr5X5GR1vK2dw57kmBKc Figure 1C rows 2 and 3, note chromosome missegregation in the cell of row 3, and see Supplementary Figure S1 for quantifica- tion of spindle intensity ------- COMMENT: a39841010d3ec10f 10 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: a39841010d3ec10f 11 iAcbpl1ew+nFwb8AiAqOLZxpvN8 Figure 1D ------- COMMENT: a39841010d3ec10f 12 iAcbpl1ew+nFwb8AiAqOLZxpvN8 Figure 1D ------- COMMENT: a39841010d3ec10f 13 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: a39841010d3ec10f 14 MpUPCfz+cLLC02XlUuLxi0SdM6M On temperature shift down to 20°C, Alp7-YFP localized only to the SPB (Figure 2B). This also indicates that Alp7 does not require a microtubule cytoskeleton to localize to the SPB. ------- COMMENT: a39841010d3ec10f 15 Mc1Dl/8ePjG+W3BQIevC1vaEvqw Figure 2D ------- COMMENT: a39841010d3ec10f 16 iAcbpl1ew+nFwb8AiAqOLZxpvN8 Figure 1D ------- COMMENT: a39841010d3ec10f 17 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: a39841010d3ec10f 18 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: a39841010d3ec10f 19 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: a39841010d3ec10f 20 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: a39841010d3ec10f 21 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: a39841010d3ec10f 22 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: a39841010d3ec10f 23 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: a39841010d3ec10f 24 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: a39841010d3ec10f 25 wf185u4DRDkg7Sbpsn3gLe6+2NY Figure 4E ------- COMMENT: a39841010d3ec10f 26 5IGc3difXx4+SJNj9Vf3lGyEbhs Figure 4F ------- COMMENT: a39841010d3ec10f 27 SLs5aXgEIyO20i/u/O4jUYXx2z8 Figure 5A, lane 4 ------- COMMENT: a39841010d3ec10f 28 SLs5aXgEIyO20i/u/O4jUYXx2z8 Figure 5A, lane 4 ------- COMMENT: a39841010d3ec10f 29 bQrAvw5v3pdeGEjTasNZSNQl2dw Figure 6A ------- COMMENT: a39841010d3ec10f 30 bQrAvw5v3pdeGEjTasNZSNQl2dw Figure 6A ------- COMMENT: a39841010d3ec10f 31 5Leuj0nQtQ/oH58v8XLkYXSDMog Figure 6B ------- COMMENT: a39841010d3ec10f 32 TIUZrzT9Y0GaidscfJ9Hf9+bnS8 Fig. 8C ------- COMMENT: a39841010d3ec10f 35 wM1j2mH6/TW+/5OVQ9k1Yx4RjSs Figure 8E ------- COMMENT: a39841010d3ec10f 36 wM1j2mH6/TW+/5OVQ9k1Yx4RjSs Figure 8E ------- COMMENT: a39841010d3ec10f 37 wM1j2mH6/TW+/5OVQ9k1Yx4RjSs Figure 8E ------- COMMENT: a39841010d3ec10f 38 wM1j2mH6/TW+/5OVQ9k1Yx4RjSs Figure 8E ------- COMMENT: a39841010d3ec10f 39 wM1j2mH6/TW+/5OVQ9k1Yx4RjSs Figure 8E ------- COMMENT: a3a97d79735bef9a 22 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 23 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 24 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 25 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 26 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 27 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 28 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 29 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 30 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 31 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 32 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 33 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 34 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 35 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 36 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 37 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 38 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 39 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 40 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 41 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 42 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 43 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 44 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 45 BAe0oolihMKfWC0lCiTZEN31Fn8 correlation with gel shift assays ------- COMMENT: a3a97d79735bef9a 48 fCBY0Q8gypVkJdUR5GVKRwcmB4k co-crystal structure of Pho7 DNA binding domain with the pho7 binding site in the tgp1 promoter; DNA binding domain determined to be aa279-336; structure shows the monomeric DNA binding domain co-ordinating two zinc atoms with a Zn2Cys6 domain architecture (similar to Gal4); Structure shows all protein-DNA contacts ------- COMMENT: a3ae4b87ad040aa7 1 kws7dmtH8vmM7O6TeyEIlqrqXGM (comment: independent of F-actin (assayed using Latrunculin A)) ------- COMMENT: a3ae4b87ad040aa7 2 goEO9S3UHQnaYolcF2Vye6cN6UI (comment: dependent on F-actin (assayed using Latrunculin A)) ------- COMMENT: a3ae4b87ad040aa7 3 goEO9S3UHQnaYolcF2Vye6cN6UI (comment: dependent on F-actin (assayed using Latrunculin A)) ------- COMMENT: a3ae4b87ad040aa7 4 goEO9S3UHQnaYolcF2Vye6cN6UI (comment: dependent on F-actin (assayed using Latrunculin A)) ------- COMMENT: a3ae4b87ad040aa7 5 goEO9S3UHQnaYolcF2Vye6cN6UI (comment: dependent on F-actin (assayed using Latrunculin A)) ------- COMMENT: a3ae4b87ad040aa7 6 tysqQ+WkMtJBI0O87/5WtfpPcuo (comment: dependent on F-actin (assayed using Latruncilin A)) ------- COMMENT: a3ae4b87ad040aa7 7 goEO9S3UHQnaYolcF2Vye6cN6UI (comment: dependent on F-actin (assayed using Latrunculin A)) ------- COMMENT: a3ae4b87ad040aa7 8 goEO9S3UHQnaYolcF2Vye6cN6UI (comment: dependent on F-actin (assayed using Latrunculin A)) ------- COMMENT: a3ae4b87ad040aa7 11 aDwilgrxMcd2Tcy9YM8LwfYTm34 (comment: late interphase; independent of F-actin (assayed using Latrunculin A)) ------- COMMENT: a3ae4b87ad040aa7 12 aDwilgrxMcd2Tcy9YM8LwfYTm34 (comment: late interphase; independent of F-actin (assayed using Latrunculin A)) ------- COMMENT: a3ae4b87ad040aa7 13 aDwilgrxMcd2Tcy9YM8LwfYTm34 (comment: late interphase; independent of F-actin (assayed using Latrunculin A)) ------- COMMENT: a3ae4b87ad040aa7 14 aDwilgrxMcd2Tcy9YM8LwfYTm34 (comment: late interphase; independent of F-actin (assayed using Latrunculin A)) ------- COMMENT: a3ae4b87ad040aa7 15 aDwilgrxMcd2Tcy9YM8LwfYTm34 (comment: late interphase; independent of F-actin (assayed using Latrunculin A)) ------- COMMENT: a3ae4b87ad040aa7 16 ji8/lNkv8d3LSHFtimVdxLCsiqQ (comment: before late interphase) ------- COMMENT: a3ae4b87ad040aa7 17 aDwilgrxMcd2Tcy9YM8LwfYTm34 (comment: late interphase; independent of F-actin (assayed using Latrunculin A)) ------- COMMENT: a3ae4b87ad040aa7 19 aDwilgrxMcd2Tcy9YM8LwfYTm34 (comment: late interphase; independent of F-actin (assayed using Latrunculin A)) ------- COMMENT: a3ae4b87ad040aa7 20 aDwilgrxMcd2Tcy9YM8LwfYTm34 (comment: late interphase; independent of F-actin (assayed using Latrunculin A)) ------- COMMENT: a3ae4b87ad040aa7 21 aDwilgrxMcd2Tcy9YM8LwfYTm34 (comment: late interphase; independent of F-actin (assayed using Latrunculin A)) ------- COMMENT: a3ae4b87ad040aa7 22 aDwilgrxMcd2Tcy9YM8LwfYTm34 (comment: late interphase; independent of F-actin (assayed using Latrunculin A)) ------- COMMENT: a3ce872c46b27485 10 z/qISWvrRLf6pChAqYskTqvBD38 inferred from normal localization of CMG proteins at origin ------- COMMENT: a3ce872c46b27485 11 OVl8mbEx2lEW7GhurI0Z0Bb6WIA BrdU incorporation ------- COMMENT: a3ce872c46b27485 13 5TU1nVxL6C5NYMjzDdzYrphx+Cw inferred from localization of proteins distal to origin ------- COMMENT: a3ce872c46b27485 18 OVl8mbEx2lEW7GhurI0Z0Bb6WIA BrdU incorporation ------- COMMENT: a3ce872c46b27485 21 5TU1nVxL6C5NYMjzDdzYrphx+Cw inferred from localization of proteins distal to origin ------- COMMENT: a3ce872c46b27485 29 OVl8mbEx2lEW7GhurI0Z0Bb6WIA BrdU incorporation ------- COMMENT: a3ce872c46b27485 33 5TU1nVxL6C5NYMjzDdzYrphx+Cw inferred from localization of proteins distal to origin ------- COMMENT: a3ce872c46b27485 36 5TU1nVxL6C5NYMjzDdzYrphx+Cw inferred from localization of proteins distal to origin ------- COMMENT: a3ce872c46b27485 41 OVl8mbEx2lEW7GhurI0Z0Bb6WIA BrdU incorporation ------- COMMENT: a3d4e7143693a96d 1 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 2 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 3 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 4 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 5 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 6 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 7 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 8 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 9 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 10 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 11 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 12 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 13 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 14 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 15 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 16 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 17 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 18 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 19 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 20 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 21 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 22 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 23 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 24 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 25 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 26 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 27 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 28 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 29 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a3d4e7143693a96d 30 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: a3d4e7143693a96d 31 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a3d4e7143693a96d 32 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a3d4e7143693a96d 33 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a3d4e7143693a96d 34 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a3d4e7143693a96d 35 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a3d4e7143693a96d 36 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a3d4e7143693a96d 38 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a3d4e7143693a96d 39 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a3d4e7143693a96d 40 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: a3d4e7143693a96d 41 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a3d4e7143693a96d 42 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: a3d4e7143693a96d 43 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: a3d4e7143693a96d 44 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: a3de75bdb576bfd5 1 AQKgJcgDVW72a3eCHixrlXHPv98 (comment: erratum corrected previous annotation) ------- COMMENT: a40d7474e17bb4c0 2 rJBdj4Iht6VwXcOzn/xnjlEhsQA Thus, Alm1 and Nup60 promote RDR in a pre- and post-anchoring manner, respectively. ------- COMMENT: a40d7474e17bb4c0 3 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: a40d7474e17bb4c0 4 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: a40d7474e17bb4c0 5 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: a40d7474e17bb4c0 6 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: a40d7474e17bb4c0 7 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: a40d7474e17bb4c0 8 9NbdUBVwEIM5E75sjWyhsYxnrWU Fig. 6A and D ------- COMMENT: a40d7474e17bb4c0 9 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: a40d7474e17bb4c0 10 u7vB737cal6u9a+rck5c9M4tNA4 Fig. 5D and E ------- COMMENT: a40d7474e17bb4c0 11 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: a40d7474e17bb4c0 12 mp0M7zDIFggn53e2MCluP++FNgs Fig. 5E ------- COMMENT: a40d7474e17bb4c0 13 xLld+U4XtO1Yq5XJ/vA+lPgihjU Fig. 6D ------- COMMENT: a40d7474e17bb4c0 14 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: a40d7474e17bb4c0 20 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: a40d7474e17bb4c0 21 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: a40d7474e17bb4c0 22 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: a40d7474e17bb4c0 23 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: a40d7474e17bb4c0 24 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: a40d7474e17bb4c0 25 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: a40d7474e17bb4c0 26 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: a40d7474e17bb4c0 27 PtWz3QLtFNOk+hQdKIzxMwXnm5Q Fig. 3A and B ------- COMMENT: a40d7474e17bb4c0 28 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: a40d7474e17bb4c0 29 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: a40d7474e17bb4c0 30 ZSoIdAAkba/kthzeLNn7KxqZ/Bw Fig. S6B ------- COMMENT: a40d7474e17bb4c0 31 Zno40JIIfMde9u5cNVEbdVzh+iw Fig. 1B and C ------- COMMENT: a40d7474e17bb4c0 32 4JQt0wN2TH7w8wc2RC2zx0BwanM Fig. S1A ------- COMMENT: a40d7474e17bb4c0 33 4JQt0wN2TH7w8wc2RC2zx0BwanM Fig. S1A ------- COMMENT: a40d7474e17bb4c0 34 4JQt0wN2TH7w8wc2RC2zx0BwanM Fig. S1A ------- COMMENT: a40d7474e17bb4c0 35 4JQt0wN2TH7w8wc2RC2zx0BwanM Fig. S1A ------- COMMENT: a40d7474e17bb4c0 36 4JQt0wN2TH7w8wc2RC2zx0BwanM Fig. S1A ------- COMMENT: a40d7474e17bb4c0 37 4JQt0wN2TH7w8wc2RC2zx0BwanM Fig. S1A ------- COMMENT: a40d7474e17bb4c0 38 4JQt0wN2TH7w8wc2RC2zx0BwanM Fig. S1A ------- COMMENT: a40d7474e17bb4c0 39 4JQt0wN2TH7w8wc2RC2zx0BwanM Fig. S1A ------- COMMENT: a40d7474e17bb4c0 40 4JQt0wN2TH7w8wc2RC2zx0BwanM Fig. S1A ------- COMMENT: a40d7474e17bb4c0 41 4JQt0wN2TH7w8wc2RC2zx0BwanM Fig. S1A ------- COMMENT: a40d7474e17bb4c0 42 AJM3UzZfcNA51z7jraSDCDOL5LY Fig. S1B ------- COMMENT: a40d7474e17bb4c0 43 AJM3UzZfcNA51z7jraSDCDOL5LY Fig. S1B ------- COMMENT: a40d7474e17bb4c0 44 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: a40d7474e17bb4c0 45 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: a40d7474e17bb4c0 46 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: a40d7474e17bb4c0 47 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: a40d7474e17bb4c0 48 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: a40d7474e17bb4c0 49 ifrqlcy7G0f4EFipSEbm7b0gMNU Fig. S2A ------- COMMENT: a40d7474e17bb4c0 50 ifrqlcy7G0f4EFipSEbm7b0gMNU Fig. S2A ------- COMMENT: a40d7474e17bb4c0 51 ifrqlcy7G0f4EFipSEbm7b0gMNU Fig. S2A ------- COMMENT: a40d7474e17bb4c0 52 ifrqlcy7G0f4EFipSEbm7b0gMNU Fig. S2A ------- COMMENT: a40d7474e17bb4c0 53 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: a40d7474e17bb4c0 54 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: a40d7474e17bb4c0 55 JzjGSncvho67kPPmO3S/jqEqvk0 The Ulp1 SUMO protease ensures an efficient initiation of restarted DNA synthesis. This mechanism requires the sequestration of Ulp1 at the NP which is coordinated by the Y complex and the nuclear basket nucleoporin Nup60 ------- COMMENT: a40d7474e17bb4c0 56 JzjGSncvho67kPPmO3S/jqEqvk0 The Ulp1 SUMO protease ensures an efficient initiation of restarted DNA synthesis. This mechanism requires the sequestration of Ulp1 at the NP which is coordinated by the Y complex and the nuclear basket nucleoporin Nup60 ------- COMMENT: a40d7474e17bb4c0 57 JzjGSncvho67kPPmO3S/jqEqvk0 The Ulp1 SUMO protease ensures an efficient initiation of restarted DNA synthesis. This mechanism requires the sequestration of Ulp1 at the NP which is coordinated by the Y complex and the nuclear basket nucleoporin Nup60 ------- COMMENT: a42509fe62ac69ca 31 u1igVeBZct7DfZqMWg/JXrZsWOw (comment: indicates a G2 delay) ------- COMMENT: a4361279dd5b39bb 1 uVCVOJsEpFkEU4bAoAnyTLogHL0 Figure 1B ------- COMMENT: a4361279dd5b39bb 2 uVCVOJsEpFkEU4bAoAnyTLogHL0 Figure 1B ------- COMMENT: a4361279dd5b39bb 3 uVCVOJsEpFkEU4bAoAnyTLogHL0 Figure 1B ------- COMMENT: a4361279dd5b39bb 6 /j3HmmmiVY0Ai07WYAm0hRFB00k Supplemental data ------- COMMENT: a4361279dd5b39bb 9 uVCVOJsEpFkEU4bAoAnyTLogHL0 Figure 1B ------- COMMENT: a4361279dd5b39bb 10 uVCVOJsEpFkEU4bAoAnyTLogHL0 Figure 1B ------- COMMENT: a4361279dd5b39bb 20 myxbYBU7m8nPVLH5GDyqAnU8Sk4 (comment: morphology) ------- COMMENT: a4361279dd5b39bb 21 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: a4361279dd5b39bb 22 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: a4361279dd5b39bb 23 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: a4361279dd5b39bb 24 QOimfNEgIg0vlR1UCc9yZ3fghL4 fig 5d ------- COMMENT: a4361279dd5b39bb 25 mrzFP8HqSEhkWH0BkIHE+1FHffk fig 5e ------- COMMENT: a4361279dd5b39bb 27 shW5yeEDDVYLJkTilfIO5ap39Jo table 4 ------- COMMENT: a4361279dd5b39bb 28 shW5yeEDDVYLJkTilfIO5ap39Jo table 4 ------- COMMENT: a4361279dd5b39bb 29 shW5yeEDDVYLJkTilfIO5ap39Jo table 4 ------- COMMENT: a4361279dd5b39bb 30 shW5yeEDDVYLJkTilfIO5ap39Jo table 4 ------- COMMENT: a4361279dd5b39bb 31 shW5yeEDDVYLJkTilfIO5ap39Jo table 4 ------- COMMENT: a4361279dd5b39bb 33 FD+jsQtkRMOECvlJlVrlOQfJqd0 Figur 6B, C ------- COMMENT: a4361279dd5b39bb 34 FD+jsQtkRMOECvlJlVrlOQfJqd0 Figur 6B, C ------- COMMENT: a4361279dd5b39bb 35 qtZLxTGc4oVhD6PzyM79iubQnj8 fig 7b ------- COMMENT: a4361279dd5b39bb 36 hX7vaTi1BRs3/LhLfgU7aL6G+rs fig 7a ------- COMMENT: a4361279dd5b39bb 37 hX7vaTi1BRs3/LhLfgU7aL6G+rs fig 7a ------- COMMENT: a4361279dd5b39bb 40 GUEAo5UF/ckyPJHgHNsC+q/4mB4 Figure 8A, B ------- COMMENT: a4361279dd5b39bb 41 oUIsPa1LvwuSlMzCPy+FhvtsRvQ Figure 8C ------- COMMENT: a4361279dd5b39bb 42 yEV8zSLmgX2rWCqNwlItgKKdq80 Figure 8D ------- COMMENT: a4361279dd5b39bb 44 yEV8zSLmgX2rWCqNwlItgKKdq80 Figure 8D ------- COMMENT: a4361279dd5b39bb 45 yEV8zSLmgX2rWCqNwlItgKKdq80 Figure 8D ------- COMMENT: a4361279dd5b39bb 46 pC4KdgYYDOIwlfWjWv9ATkCNTqQ Figure 9 ------- COMMENT: a4361279dd5b39bb 47 J2w95/Cs2hhrV4dKD3Q8YH9/l6U both the growth and shrinkage rates were decreased down to 33 and 60%, respectively, ------- COMMENT: a4361279dd5b39bb 48 J2w95/Cs2hhrV4dKD3Q8YH9/l6U both the growth and shrinkage rates were decreased down to 33 and 60%, respectively, ------- COMMENT: a4361279dd5b39bb 49 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: a4361279dd5b39bb 50 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: a4361279dd5b39bb 54 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: a4361279dd5b39bb 55 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: a4361279dd5b39bb 56 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: a475a61f06526c04 1 gm1Px94b5UBdjxqoHSq63NZdxOQ table1 ------- COMMENT: a475a61f06526c04 2 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a475a61f06526c04 3 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a475a61f06526c04 4 V8aY9cMSuflWPDnZXK1Ej8D/EEk fig 1b ------- COMMENT: a475a61f06526c04 5 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a475a61f06526c04 6 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a475a61f06526c04 7 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a475a61f06526c04 8 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a475a61f06526c04 9 gm1Px94b5UBdjxqoHSq63NZdxOQ table1 ------- COMMENT: a475a61f06526c04 10 gm1Px94b5UBdjxqoHSq63NZdxOQ table1 ------- COMMENT: a475a61f06526c04 11 gm1Px94b5UBdjxqoHSq63NZdxOQ table1 ------- COMMENT: a475a61f06526c04 12 gm1Px94b5UBdjxqoHSq63NZdxOQ table1 ------- COMMENT: a475a61f06526c04 13 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a475a61f06526c04 14 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a475a61f06526c04 15 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a475a61f06526c04 16 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a475a61f06526c04 17 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a475a61f06526c04 18 gm1Px94b5UBdjxqoHSq63NZdxOQ table1 ------- COMMENT: a475a61f06526c04 19 gm1Px94b5UBdjxqoHSq63NZdxOQ table1 ------- COMMENT: a475a61f06526c04 21 9CG7PpA7NLedIuW/VVp4ASqyadk fig 1d ie not blocked in g2 ------- COMMENT: a475a61f06526c04 23 Unxy2Chsi3AwbN/o8dyNh1X91iY Fig. 1e, Fig. 1f) ------- COMMENT: a475a61f06526c04 24 9z7jP/2MkjgBT0yuKquAWVzklC8 Fig. 1e in vitro link from epistastis and delayed cdc2 phosphorylation ------- COMMENT: a475a61f06526c04 25 cY1xY2Rk9ie7I4p27WqS3ghCM4s fig 1g ------- COMMENT: a475a61f06526c04 26 WA6sLzlO1oTZdARG+gt299Uk67g Supplementary Fig. 2 ------- COMMENT: a475a61f06526c04 27 WA6sLzlO1oTZdARG+gt299Uk67g Supplementary Fig. 2 ------- COMMENT: a475a61f06526c04 28 MDXsX/DFqszsnj94DJ8wcIA4tu4 Supplementary Fig. 3 ------- COMMENT: a475a61f06526c04 29 MDXsX/DFqszsnj94DJ8wcIA4tu4 Supplementary Fig. 3 ------- COMMENT: a475a61f06526c04 30 4Zqzab3UzX8IHcxf3wSa0sXT23U Supplementary Fig. 4 ------- COMMENT: a475a61f06526c04 31 x9K3fAc4TktRtaGY9tA8I4n79RU Fig. 2a and data not shown ------- COMMENT: a475a61f06526c04 32 x9K3fAc4TktRtaGY9tA8I4n79RU Fig. 2a and data not shown ------- COMMENT: a475a61f06526c04 33 x9K3fAc4TktRtaGY9tA8I4n79RU Fig. 2a and data not shown ------- COMMENT: a475a61f06526c04 35 iD/ywZFp3LP2EzYpaZhepDbAXSQ (comment: cortex) ------- COMMENT: a475a61f06526c04 36 V8aY9cMSuflWPDnZXK1Ej8D/EEk fig 1b ------- COMMENT: a475a61f06526c04 37 V8aY9cMSuflWPDnZXK1Ej8D/EEk fig 1b ------- COMMENT: a475a61f06526c04 38 e5BV2ogMLcyUeKKgzkDo71CmjQs (comment: or is this reduced with low exressivity?) ------- COMMENT: a475a61f06526c04 39 JCGsnmNjBQbDK0l2f7XMZJZv5+A Table 1, Fig. 2d ------- COMMENT: a475a61f06526c04 40 JCGsnmNjBQbDK0l2f7XMZJZv5+A Table 1, Fig. 2d ------- COMMENT: a475a61f06526c04 41 JCGsnmNjBQbDK0l2f7XMZJZv5+A Table 1, Fig. 2d ------- COMMENT: a475a61f06526c04 43 jpKG02Lq3+wnUQTjQpeWybmuDMM (Fig. 3b). ------- COMMENT: a475a61f06526c04 44 jpKG02Lq3+wnUQTjQpeWybmuDMM (Fig. 3b). ------- COMMENT: a475a61f06526c04 45 jpKG02Lq3+wnUQTjQpeWybmuDMM (Fig. 3b). ------- COMMENT: a475a61f06526c04 46 jpKG02Lq3+wnUQTjQpeWybmuDMM (Fig. 3b). ------- COMMENT: a475a61f06526c04 47 jpKG02Lq3+wnUQTjQpeWybmuDMM (Fig. 3b). ------- COMMENT: a475a61f06526c04 48 9z7jP/2MkjgBT0yuKquAWVzklC8 Fig. 1e in vitro link from epistastis and delayed cdc2 phosphorylation ------- COMMENT: a475b51d06fe1b16 4 BdAhtQ4o7DA0nIGWiJtZMb55oTI Good evidence for this is the colocalisation with cnp3 in Fig. 3A in the csi1D background, where alp7 does not go to the spindle, or nda3 mutant where the spindle does not form, but alp7 still goes to the kinetochore, as seen by cnp3 ------- COMMENT: a475b51d06fe1b16 6 Se4hEWISAA0ZpOyCWIA1KyjPDwQ Thorough experiments throughout using both full deletions as well as phospho mutants. Direct physical interaction is confirmed by Y2H ------- COMMENT: a475b51d06fe1b16 7 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a475b51d06fe1b16 8 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a475b51d06fe1b16 9 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a475b51d06fe1b16 12 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: a475b51d06fe1b16 13 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: a475b51d06fe1b16 14 FeX3nNhC/gudGSWvsa359Zi1Hgk Fig. S1D ------- COMMENT: a475b51d06fe1b16 15 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: a475b51d06fe1b16 16 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: a475b51d06fe1b16 17 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: a475b51d06fe1b16 18 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a475b51d06fe1b16 19 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a475b51d06fe1b16 20 8qcE6r5/VcApJKkdLLqCS4XB93w Fig. 4 (comment: BiFC) ------- COMMENT: a475b51d06fe1b16 21 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a475b51d06fe1b16 22 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a475b51d06fe1b16 23 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a475b51d06fe1b16 24 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a475b51d06fe1b16 25 8qcE6r5/VcApJKkdLLqCS4XB93w Fig. 4 (comment: BiFC) ------- COMMENT: a475b51d06fe1b16 26 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a475b51d06fe1b16 27 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a475b51d06fe1b16 28 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a475b51d06fe1b16 29 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a475b51d06fe1b16 30 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a475b51d06fe1b16 31 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a475b51d06fe1b16 32 PVhL0V3OV06fjEegM5fWNW/f9SI Fig. S5D ------- COMMENT: a475b51d06fe1b16 33 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: a475b51d06fe1b16 34 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: a475b51d06fe1b16 35 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: a493fe4ede17fe68 1 bQLIj7hPPSpprNk8NYFg1PoGsZc Fig. 4B The dga1Δplh1Δ mutant possessed few lipid droplets. ------- COMMENT: a493fe4ede17fe68 2 FWCOqMkS6ERKjAeHaEuKRNmPKSc Fig. 4A, 4C, and 4D Using spore colony formation assay and microscopic observation, most of the dga1Δplh1Δ mutant spores failed to form colonies showed no sign of germination. ------- COMMENT: a493fe4ede17fe68 3 bQLIj7hPPSpprNk8NYFg1PoGsZc Fig. 4B The dga1Δplh1Δ mutant possessed few lipid droplets. ------- COMMENT: a493fe4ede17fe68 4 r/y9NE6LcAjau9VVcwvVMY/sooY Fig. 4F Isp3-GFP was improperly assembled in the dga1Δplh1Δ mutant. ------- COMMENT: a493fe4ede17fe68 6 cZm8FA11m8mbMEYFNq2mQLNtVaU Fig. 1F, Fig. 2C ------- COMMENT: a493fe4ede17fe68 7 bTqC8VYzj0ig5CIU/5R9F7cZRxw Fig. 1H, Fig. 3B ------- COMMENT: a493fe4ede17fe68 8 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: a493fe4ede17fe68 9 qZorvNd55HXcALMv1Q7hLAOSzgE Fig. 4F Assembly of Isp3-GFP onto the spore surface was defective in the dga1Δplh1Δ mutant. ------- COMMENT: a493fe4ede17fe68 10 j2SG4+FGU82dUT//jcUUlN5XK/c figure S2 ------- COMMENT: a493fe4ede17fe68 11 j2SG4+FGU82dUT//jcUUlN5XK/c figure S2 ------- COMMENT: a493fe4ede17fe68 12 SDmhJVEaY4D9YZ/VRn+lI/mR0Ug Figure S3 LP clustering at nucleus ------- COMMENT: a493fe4ede17fe68 13 0cn96O+PUHoWOuDN0dy+AYGNHtA Fig. 2C ------- COMMENT: a493fe4ede17fe68 14 YN2yTrJO5Uo2fqdyZyApQJw/joc Fig. 2C (comment: also in lantrunculin treated sceels indicating actin dependeny) ------- COMMENT: a493fe4ede17fe68 15 YN2yTrJO5Uo2fqdyZyApQJw/joc Fig. 2C (comment: also in lantrunculin treated sceels indicating actin dependeny) ------- COMMENT: a493fe4ede17fe68 16 YN2yTrJO5Uo2fqdyZyApQJw/joc Fig. 2C (comment: also in lantrunculin treated sceels indicating actin dependeny) ------- COMMENT: a493fe4ede17fe68 17 8mNMCYh7URBFzU5U2b1U7i4TQ5Q Fig. 3B normal lipid droplet localization to FSM leading edge ------- COMMENT: a493fe4ede17fe68 18 VukLLkbbZBWYyv0CLEX5uEoNsJY Fig. 3B (comment: also in lantrunculin treated sceels indicating actin dependeny) ------- COMMENT: a493fe4ede17fe68 20 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: a493fe4ede17fe68 21 VukLLkbbZBWYyv0CLEX5uEoNsJY Fig. 3B (comment: also in lantrunculin treated sceels indicating actin dependeny) ------- COMMENT: a493fe4ede17fe68 22 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: a493fe4ede17fe68 23 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: a493fe4ede17fe68 27 gzikuMKB/H4aFmAG2Jw7gD7YKoA Fig. 4A,C) ------- COMMENT: a4941387dd36601f 1 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 2 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 3 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: a4941387dd36601f 4 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 5 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 6 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 7 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 8 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 9 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 10 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 11 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 12 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 13 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 14 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 15 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 16 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 17 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 18 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 19 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 20 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 21 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 22 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 23 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 24 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 25 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 26 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 27 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: a4941387dd36601f 28 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 29 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 30 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 31 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 32 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 33 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 34 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 35 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 36 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 38 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 39 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 40 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 41 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 42 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 43 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 44 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 45 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 46 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 47 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 48 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 49 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 50 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 51 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 52 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 53 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: a4941387dd36601f 54 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 55 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 56 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 57 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 58 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 59 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 60 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 61 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 62 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 63 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 64 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 65 v42EApS7a04ZBZyrpK4bHDINEQM Fig. 1B, Table 1 ------- COMMENT: a4941387dd36601f 66 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: a4941387dd36601f 67 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: a4941387dd36601f 68 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: a4941387dd36601f 69 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: a4941387dd36601f 70 krSNa73S9t2LuF+otHi/az3pilA Fig. 2H ------- COMMENT: a4941387dd36601f 71 krSNa73S9t2LuF+otHi/az3pilA Fig. 2H ------- COMMENT: a4941387dd36601f 72 krSNa73S9t2LuF+otHi/az3pilA Fig. 2H ------- COMMENT: a4941387dd36601f 73 ifrqlcy7G0f4EFipSEbm7b0gMNU Fig. S2A ------- COMMENT: a4941387dd36601f 74 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: a4941387dd36601f 75 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: a4941387dd36601f 76 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: a4941387dd36601f 77 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: a4941387dd36601f 78 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: a4941387dd36601f 79 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: a4941387dd36601f 80 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: a4941387dd36601f 81 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: a4941387dd36601f 82 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: a4941387dd36601f 83 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: a4941387dd36601f 84 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: a4941387dd36601f 85 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: a4941387dd36601f 86 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: a4941387dd36601f 87 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: a4941387dd36601f 88 9Xb+uz4ftlqagKGp7TY1gJc7WdA Fig. 4F ------- COMMENT: a4941387dd36601f 89 9Xb+uz4ftlqagKGp7TY1gJc7WdA Fig. 4F ------- COMMENT: a4941387dd36601f 90 dJ55jf9F7w1w+3+W5RpemgHi8WI Fig. 5B and C ------- COMMENT: a4941387dd36601f 91 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: a4941387dd36601f 92 mp0M7zDIFggn53e2MCluP++FNgs Fig. 5E ------- COMMENT: a4941387dd36601f 93 cLKKHHathQCT1JZTf0i7RHzTu8A Fig. 5F ------- COMMENT: a4941387dd36601f 94 cLKKHHathQCT1JZTf0i7RHzTu8A Fig. 5F ------- COMMENT: a4941387dd36601f 95 cLKKHHathQCT1JZTf0i7RHzTu8A Fig. 5F ------- COMMENT: a4941387dd36601f 96 T44vR21/o+u/qtfx00leUPdjBlI Fig. 5G ------- COMMENT: a4941387dd36601f 97 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: a4941387dd36601f 98 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: a4941387dd36601f 99 YUvhVmsK5HihxtoTGdMDWBN3eC0 Fig. 6F and G ------- COMMENT: a4941387dd36601f 100 YUvhVmsK5HihxtoTGdMDWBN3eC0 Fig. 6F and G ------- COMMENT: a4941387dd36601f 101 /EKJDn1kiFepVVy1Uoj4agCFYLE Fig. 6E ------- COMMENT: a4941387dd36601f 102 /EKJDn1kiFepVVy1Uoj4agCFYLE Fig. 6E ------- COMMENT: a4941387dd36601f 103 /EKJDn1kiFepVVy1Uoj4agCFYLE Fig. 6E ------- COMMENT: a4941387dd36601f 104 /EKJDn1kiFepVVy1Uoj4agCFYLE Fig. 6E ------- COMMENT: a4941387dd36601f 105 clW0KYjVSszqiWZZf78RB8Z3Z+o Fig. 6H ------- COMMENT: a4941387dd36601f 106 clW0KYjVSszqiWZZf78RB8Z3Z+o Fig. 6H ------- COMMENT: a4941387dd36601f 107 clW0KYjVSszqiWZZf78RB8Z3Z+o Fig. 6H ------- COMMENT: a4941387dd36601f 108 clW0KYjVSszqiWZZf78RB8Z3Z+o Fig. 6H ------- COMMENT: a4ab4922ffecbd92 1 SuAKpBDEMJNJNTZKxrwukiJN+ZM (Figure 1, C and D). ------- COMMENT: a4ab4922ffecbd92 3 /lc8MZKd4MJlYeNvCfre+ZzabcM (comment: mixed orientations) ------- COMMENT: a4ab4922ffecbd92 4 rOyYE3MaButXPq0Csrb/T04ZhLo (Figure 3A and Supplemental Video 3). ------- COMMENT: a4ab4922ffecbd92 7 /y3mItgcyZ/sSWGITB1oNw59IYA Fig. 6C ------- COMMENT: a4ab4922ffecbd92 8 /y3mItgcyZ/sSWGITB1oNw59IYA Fig. 6C ------- COMMENT: a4ab4922ffecbd92 9 /y3mItgcyZ/sSWGITB1oNw59IYA Fig. 6C ------- COMMENT: a4ab4922ffecbd92 10 /y3mItgcyZ/sSWGITB1oNw59IYA Fig. 6C ------- COMMENT: a4ab4922ffecbd92 11 /y3mItgcyZ/sSWGITB1oNw59IYA Fig. 6C ------- COMMENT: a4ab4922ffecbd92 12 /y3mItgcyZ/sSWGITB1oNw59IYA Fig. 6C ------- COMMENT: a4bdcc99861b6595 3 mJ9CMFYJ1LkGShGfHdjSKUS9SnY Heterochromatin structure protects native CENP-A from ubiquitin-mediated degradation. ------- COMMENT: a4bdcc99861b6595 4 EVJT3NpMKoMwrnVs7ZnNzWWXSms (comment: CHECK polyubiquitylated) ------- COMMENT: a4cb97c63c8d6665 1 bsPswdkj+kKOrupDwCy/bFXakTk figS1B ------- COMMENT: a4cb97c63c8d6665 2 bsPswdkj+kKOrupDwCy/bFXakTk figS1B ------- COMMENT: a4cb97c63c8d6665 3 bsPswdkj+kKOrupDwCy/bFXakTk figS1B ------- COMMENT: a4cb97c63c8d6665 4 bsPswdkj+kKOrupDwCy/bFXakTk figS1B ------- COMMENT: a4cb97c63c8d6665 5 bsPswdkj+kKOrupDwCy/bFXakTk figS1B ------- COMMENT: a4cb97c63c8d6665 6 bsPswdkj+kKOrupDwCy/bFXakTk figS1B ------- COMMENT: a4cb97c63c8d6665 7 bsPswdkj+kKOrupDwCy/bFXakTk figS1B ------- COMMENT: a4cb97c63c8d6665 8 bsPswdkj+kKOrupDwCy/bFXakTk figS1B ------- COMMENT: a4cb97c63c8d6665 9 bsPswdkj+kKOrupDwCy/bFXakTk figS1B ------- COMMENT: a4cb97c63c8d6665 10 bsPswdkj+kKOrupDwCy/bFXakTk figS1B ------- COMMENT: a4cb97c63c8d6665 11 bsPswdkj+kKOrupDwCy/bFXakTk figS1B ------- COMMENT: a4cb97c63c8d6665 12 zGkA6zbGoeyx7U6t0BHtWjAr06o (Fig. 1C; supplementary material Fig. S1C), ------- COMMENT: a4cb97c63c8d6665 13 zGkA6zbGoeyx7U6t0BHtWjAr06o (Fig. 1C; supplementary material Fig. S1C), ------- COMMENT: a4cb97c63c8d6665 14 zGkA6zbGoeyx7U6t0BHtWjAr06o (Fig. 1C; supplementary material Fig. S1C), ------- COMMENT: a4cb97c63c8d6665 19 j2SG4+FGU82dUT//jcUUlN5XK/c figure S2 ------- COMMENT: a4cb97c63c8d6665 20 j2SG4+FGU82dUT//jcUUlN5XK/c figure S2 ------- COMMENT: a4cb97c63c8d6665 24 5SLq4jjUZx4o2fqO4NJ9786gpN0 Figure S2C ------- COMMENT: a4cb97c63c8d6665 25 5SLq4jjUZx4o2fqO4NJ9786gpN0 Figure S2C ------- COMMENT: a4cb97c63c8d6665 32 NzmyXQAyDmr5/8R1tQkhqp4lRBk accumulates on shrinking microtubules Fig. 2B ------- COMMENT: a4cb97c63c8d6665 33 NzmyXQAyDmr5/8R1tQkhqp4lRBk accumulates on shrinking microtubules Fig. 2B ------- COMMENT: a4cb97c63c8d6665 34 NzmyXQAyDmr5/8R1tQkhqp4lRBk (comment: accumulates on shrinking microtubules) Fig. 2B ------- COMMENT: a4cb97c63c8d6665 35 4m2b+MaZ22yAOwYtShi4R6jBkn4 fig 3B, Fig. S3B) ------- COMMENT: a4cb97c63c8d6665 36 d33lUFN5LSU4EQV90o/1rAslVp0 (comment: CHECK microtubule cortical anchor (microtubule site clamp) add to other dynactin complex) ------- COMMENT: a4cb97c63c8d6665 37 kuS4rPa6QTzfUFuVWd8/vMHFZ9o fig 3B, Fig. S3B) abolished microtubule cortical anchoring ------- COMMENT: a4cb97c63c8d6665 38 CYx4n/NExzub3aafv6O7QXtdHXQ fig 4A ------- COMMENT: a4cb97c63c8d6665 41 CdGJkpdT8o7O/3Fn7WIGSS+xLOk Fig. S4A ------- COMMENT: a4cb97c63c8d6665 42 CdGJkpdT8o7O/3Fn7WIGSS+xLOk Fig. S4A ------- COMMENT: a4cb97c63c8d6665 43 CdGJkpdT8o7O/3Fn7WIGSS+xLOk Fig. S4A ------- COMMENT: a4cb97c63c8d6665 44 CdGJkpdT8o7O/3Fn7WIGSS+xLOk Fig. S4A ------- COMMENT: a4cb97c63c8d6665 45 PcUbyJ6I19w6EmtjwOn9CwuvQTg Fig. S4B ------- COMMENT: a4cb97c63c8d6665 46 PcUbyJ6I19w6EmtjwOn9CwuvQTg Fig. S4B ------- COMMENT: a4cb97c63c8d6665 47 38Oe7UIIZ6RhG2xBVPE5lJ+dwBQ Fig. 5C ------- COMMENT: a4cb97c63c8d6665 48 38Oe7UIIZ6RhG2xBVPE5lJ+dwBQ Fig. 5C ------- COMMENT: a4cb97c63c8d6665 49 PcUbyJ6I19w6EmtjwOn9CwuvQTg Fig. S4B ------- COMMENT: a4cb97c63c8d6665 50 PcUbyJ6I19w6EmtjwOn9CwuvQTg Fig. S4B ------- COMMENT: a4cb97c63c8d6665 51 mryEKeamiiFsLauzGAd/eLfktr4 fig 5c ------- COMMENT: a4cb97c63c8d6665 52 mryEKeamiiFsLauzGAd/eLfktr4 fig 5c ------- COMMENT: a4cb97c63c8d6665 53 MiKUzpxbTMApPIFioLvn5RWvEiY fig 6a ------- COMMENT: a4cb97c63c8d6665 54 MiKUzpxbTMApPIFioLvn5RWvEiY fig 6a ------- COMMENT: a4cb97c63c8d6665 55 MiKUzpxbTMApPIFioLvn5RWvEiY fig 6a ------- COMMENT: a4cb97c63c8d6665 56 uMNq/Emlapm5ihEyEJWHBmH28Ac abnormal movement of dynein ------- COMMENT: a4cb97c63c8d6665 57 uMNq/Emlapm5ihEyEJWHBmH28Ac abnormal movement of dynein ------- COMMENT: a4cb97c63c8d6665 58 +2XFMV1MQ4r+teF637sQosvJlkk fig 7a ------- COMMENT: a4cb97c63c8d6665 59 +2XFMV1MQ4r+teF637sQosvJlkk fig 7a ------- COMMENT: a4cb97c63c8d6665 60 u4h9s5wkcnm3kQoaW9fY2oQ7YqM fig 7A ------- COMMENT: a4cb97c63c8d6665 62 wcBnP1gJWsM+5upBzJhdMd+lvqY Fig. 7F; supplementary material Table S1 ------- COMMENT: a4cb97c63c8d6665 63 wcBnP1gJWsM+5upBzJhdMd+lvqY Fig. 7F; supplementary material Table S1 ------- COMMENT: a4cb97c63c8d6665 64 wcBnP1gJWsM+5upBzJhdMd+lvqY Fig. 7F; supplementary material Table S1 ------- COMMENT: a4cb97c63c8d6665 65 wcBnP1gJWsM+5upBzJhdMd+lvqY Fig. 7F; supplementary material Table S1 ------- COMMENT: a4cb97c63c8d6665 66 wcBnP1gJWsM+5upBzJhdMd+lvqY Fig. 7F; supplementary material Table S1 ------- COMMENT: a4cb97c63c8d6665 67 d33lUFN5LSU4EQV90o/1rAslVp0 (comment: CHECK microtubule cortical anchor (microtubule site clamp) add to other dynactin complex) ------- COMMENT: a4cb97c63c8d6665 68 d33lUFN5LSU4EQV90o/1rAslVp0 (comment: CHECK microtubule cortical anchor (microtubule site clamp) add to other dynactin complex) ------- COMMENT: a4cb97c63c8d6665 69 d33lUFN5LSU4EQV90o/1rAslVp0 (comment: CHECK microtubule cortical anchor (microtubule site clamp) add to other dynactin complex) ------- COMMENT: a4cb97c63c8d6665 70 k5vZQW1QmrbITvjJatsFxJiq7rk (comment: CHECK 3B?) ------- COMMENT: a4e15abcd73293ed 7 HIjvbpp3p9mvRXpD2eAVf3Bpj7w fig1 ER plasma membrane tethering ER-PM contact removal OR abnormal ER-PM contact formation ------- COMMENT: a4e15abcd73293ed 8 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: a4e15abcd73293ed 9 5neD5Z7BP1VfgDbZtd04ZI3HK8Q Figures 1C and S1C ER-PM contact removal ------- COMMENT: a4e15abcd73293ed 10 GIfXp2OfqVc2hqXDA1OPe+0dYRI Figures 1D and S1F ER-PM uncoupling (comment: *********lateral PM) ------- COMMENT: a4e15abcd73293ed 11 e2Bjgb03Mj/2UgrhhgWa4bhjNl0 Figures 1D and S1F ------- COMMENT: a4e15abcd73293ed 12 ViqgKuYm2rgYzfuOZm2enq9xj5c (Figure 1F) ER-PM uncoupling ------- COMMENT: a4e15abcd73293ed 13 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: a4e15abcd73293ed 14 /YjgqxTXk0IGbMcXyFsAA8D/074 Figure 2E ------- COMMENT: a4e15abcd73293ed 15 TTennTEgsaDXm1AhEBchVLMA/lc FIg 3C ------- COMMENT: a4e15abcd73293ed 16 LQmuKZbL9ND56pzzWctDT58tWRE Figure 3C ------- COMMENT: a4e15abcd73293ed 17 LQmuKZbL9ND56pzzWctDT58tWRE Figure 3C ------- COMMENT: a4e15abcd73293ed 18 iugwOICngLmkyma0auuN7ZPWnmQ Fig 3D ------- COMMENT: a4e15abcd73293ed 19 iugwOICngLmkyma0auuN7ZPWnmQ Fig 3D ------- COMMENT: a4e15abcd73293ed 20 iugwOICngLmkyma0auuN7ZPWnmQ Fig 3D ------- COMMENT: a4fee2d59c2592b2 5 L2ge8BNq4oNor6BLbQom+YqQ108 frequency of different stages of LE development is different though, but morphology is normal ------- COMMENT: a4fee2d59c2592b2 6 L2ge8BNq4oNor6BLbQom+YqQ108 frequency of different stages of LE development is different though, but morphology is normal ------- COMMENT: a509333787c2c0d7 14 qSBjrYWu2GM9/PPBTM33lMEWFGE Mitochondrial dye showed diffuse staining. they think it is a loss of membrane potential so the dye is not drawn in properly ------- COMMENT: a509333787c2c0d7 31 oI11QpDdf/JW8hUH6YrnNTFceSA Overexpression of Mtf1 and Rpo41 can induce mitochondrial transcription. Mtf1 and Rpo41 can bind and transcribe mitochondrial promoters in vitro and the initiating nucleotides were the same in vivo and in vitro. Mtf1 is required for efficient transcription. ------- COMMENT: a50d887380e58042 1 F1nSAJ3KYeKjwoIRLtsr9C3KPSs (Fig. 2H) ------- COMMENT: a50d887380e58042 2 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: a50d887380e58042 3 DES5Jkiy+rZ7wehRYgobQ94L1Q8 (Fig. 2G) ------- COMMENT: a50d887380e58042 4 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: a50d887380e58042 5 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: a50d887380e58042 6 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: a50d887380e58042 7 qte3ukxWsL9LRBz6BVnIetcVBmU (Fig. 2E and F) ------- COMMENT: a50d887380e58042 8 qte3ukxWsL9LRBz6BVnIetcVBmU (Fig. 2E and F) ------- COMMENT: a50d887380e58042 9 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: a50d887380e58042 11 F1nSAJ3KYeKjwoIRLtsr9C3KPSs (Fig. 2H) ------- COMMENT: a525406360ac556a 1 vu29XBp3zB1NjCOyxYxWbdV3clA (comment: CHECK residue=S200) ------- COMMENT: a5289f010104594b 65 VQ1ODuoKVy7hxQP9oyzyPE/4jtY Fig. 2f ------- COMMENT: a5289f010104594b 66 VQ1ODuoKVy7hxQP9oyzyPE/4jtY Fig. 2f ------- COMMENT: a5289f010104594b 67 VQ1ODuoKVy7hxQP9oyzyPE/4jtY Fig. 2f ------- COMMENT: a5289f010104594b 68 vGZEHr1uZfCurstAzPgDivKOdCM Fig. 2g ------- COMMENT: a5289f010104594b 70 rVbdDYkBekCj7BkR87EBxDRBJXY (comment: GI Redundancy) ------- COMMENT: a5af9fd629607a18 5 bL2R4DNoBep77QIIieBbUMnnzZg (comment: spatial extent ******)The assay is Ura4 expression as a reporter gene for whether heterochromatin is spreading beyond the normal boundry, which it isn't here and so the toxic analogue results in growth attenuation. But sensitivity to FOA isn't the phenotype of interest, that's just the tool ------- COMMENT: a5af9fd629607a18 6 QKlPCGMnOQG/elv2i+AoaWMSP04 (comment: spatial extent ********)" These results suggested that the 5-FOA-resistant phenotype of the original mutants was indeed due to ura4 repression, presumably as a result of heterochromatin assembly occurring outside the inverted repeat region." ------- COMMENT: a5af9fd629607a18 7 drDPvTeQLyeELY+8KFat4kfJz9A (comment: spatial extent. )" These results suggested that the 5-FOA-resistant phenotype of the original mutants was indeed due to ura4 repression, presumably as a result of heterochromatin assembly occurring outside the inverted repeat region." ------- COMMENT: a5af9fd629607a18 8 ZqReNQDIRb8WfX8ZlaOCdqAzkM4 (comment: spatial extent) ------- COMMENT: a5af9fd629607a18 9 ZqReNQDIRb8WfX8ZlaOCdqAzkM4 (comment: spatial extent) ------- COMMENT: a5af9fd629607a18 10 ZqReNQDIRb8WfX8ZlaOCdqAzkM4 (comment: spatial extent) ------- COMMENT: a5af9fd629607a18 12 oMg6KxbF+inyIs0pn/WGZuPVHO4 epigenetic variegation both 5-FOA-resistant and -sensitive colonies were found ------- COMMENT: a5af9fd629607a18 13 ZqReNQDIRb8WfX8ZlaOCdqAzkM4 (comment: spatial extent) ------- COMMENT: a5af9fd629607a18 14 ZqReNQDIRb8WfX8ZlaOCdqAzkM4 (comment: spatial extent) ------- COMMENT: a5af9fd629607a18 15 ZqReNQDIRb8WfX8ZlaOCdqAzkM4 (comment: spatial extent) ------- COMMENT: a5af9fd629607a18 16 ZqReNQDIRb8WfX8ZlaOCdqAzkM4 (comment: spatial extent) ------- COMMENT: a5f8ece090d3d081 4 nqK/xholnA/vjRSYRBIslGitWw4 present at basal level; increased in presence of hydroxyurea ------- COMMENT: a5f8ece090d3d081 5 nqK/xholnA/vjRSYRBIslGitWw4 present at basal level; increased in presence of hydroxyurea ------- COMMENT: a60531b182edb2d1 1 8jTjtkNnNoFhiY57qk4SkW6skr0 figure 1A ------- COMMENT: a60531b182edb2d1 2 jC9NkjeBIyfM2zIhNwBg6ZdMh60 figure 1C ------- COMMENT: a60531b182edb2d1 4 SVhmkydTTO9NIl82xLbHYUDlans figure 1A, 8A ------- COMMENT: a60531b182edb2d1 5 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: a60531b182edb2d1 6 kYr8Lf10VifJz44h912OtfSa33I Fig. 1D, Fig. 1F ------- COMMENT: a60531b182edb2d1 7 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: a60531b182edb2d1 8 Y/rK+VW4RXs7JXT+KWmX2SxPkAs figure 2A ------- COMMENT: a60531b182edb2d1 9 Y/rK+VW4RXs7JXT+KWmX2SxPkAs figure 2A ------- COMMENT: a60531b182edb2d1 10 Y/rK+VW4RXs7JXT+KWmX2SxPkAs figure 2A ------- COMMENT: a60531b182edb2d1 11 vckfisPDXrFks/nzYOHj1vL91d4 Fig 2F (comment: CHECK during veg phase) ------- COMMENT: a60531b182edb2d1 14 YlFlXBveMkhY2B5F35ofzrjil+I figure 8B ------- COMMENT: a60531b182edb2d1 15 YlFlXBveMkhY2B5F35ofzrjil+I figure 8B ------- COMMENT: a60531b182edb2d1 16 YlFlXBveMkhY2B5F35ofzrjil+I figure 8B ------- COMMENT: a60531b182edb2d1 17 YlFlXBveMkhY2B5F35ofzrjil+I figure 8B ------- COMMENT: a62542172341f528 1 t3RTQnOqHuDrGI8ksNNZhvWK3Z8 e, we found that more Tfg3-H formed complexes with GST–Tfg3 (Figure 1D), con- firming our hypothesis that Tfg3 forms dimers or multimers. Our observations indicated that Tfg3 is a component of TFIIF in S.pombe as it is in S.cerevisiae. ------- COMMENT: a62542172341f528 2 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: a62542172341f528 3 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: a62542172341f528 4 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: a62542172341f528 12 z2Zs0QqT9dUvEhiNHiyroDmjrMw our spores from each tetrad grew (Figure 2B) and each tetrad contained two Ura+ and two Ura spores (data not shown), indicating that tfg3+ was non- essential for cell growth under the conditions employed. ------- COMMENT: a62542172341f528 14 /QV70SzV5MNqudTd7UBoFqDVbYw At 38 C, however, the growth rate of both JY741/tfg3::ura4 and JY741/tfg3D was significantly reduced than JY741, and only small-sized colonies were detected after prolonged incubation, indicating that tfg3+ was essential for cell growth at elevated temperatures. ------- COMMENT: a62542172341f528 15 0tST1CU5iXMCnEEvX+HLL75sggc (Figure 2I). ------- COMMENT: a62542172341f528 16 Y2YBI8ykQI8UN850K14bNl8X8nI When S.pombe cells that lacked Tfg3 were streaked on a plate that contained 0.9 M KCl, they did not grow. In contrast, tfg3+ cells were able to grow (Figure 2G). ------- COMMENT: a63056e1f1822e23 1 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: a63056e1f1822e23 2 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: a63056e1f1822e23 4 wp5+HfgPgxtV8u3oUHY2Pfjmtyc Mass spectrometry analysis of purified protein mixtures associated with Flag–Clr4 [...] identified several peptides corresponding to Rik1 protein. Fig. S1 ------- COMMENT: a63056e1f1822e23 5 mfQIEnd+VAPeVpHFN0bvhvIH+rI Similarly, mass spectrometry of Flag–Rik1 affinity-purified proteins also identified Cul4 as a Rik1-interacting protein (Fig. 1f and see Supplementary Information, S1b) ------- COMMENT: a63056e1f1822e23 6 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: a63056e1f1822e23 7 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: a63056e1f1822e23 8 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: a63056e1f1822e23 9 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: a63056e1f1822e23 10 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: a63056e1f1822e23 11 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: a63056e1f1822e23 12 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: a63056e1f1822e23 13 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: a63056e1f1822e23 14 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: a63056e1f1822e23 15 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: a63056e1f1822e23 16 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: a63056e1f1822e23 17 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: a63056e1f1822e23 18 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: a63056e1f1822e23 19 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: a63056e1f1822e23 20 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: a63056e1f1822e23 21 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: a63056e1f1822e23 22 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: a63056e1f1822e23 23 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: a63056e1f1822e23 24 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: a63056e1f1822e23 25 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: a63056e1f1822e23 26 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: a63056e1f1822e23 27 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: a63056e1f1822e23 28 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: a63056e1f1822e23 29 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: a63056e1f1822e23 30 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: a63056e1f1822e23 31 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: a63056e1f1822e23 32 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: a63056e1f1822e23 33 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: a63056e1f1822e23 34 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: a63056e1f1822e23 35 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: a63056e1f1822e23 36 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: a64204abb2ff60b8 1 EcXcjJgK2p54fhWrRY0nevDLDtM To our surprise, cells lacking Top1 were resistant to H2O2 on plates, when compared to a wild-type strain (Figure 1A). ------- COMMENT: a64204abb2ff60b8 2 phzRtE8IxGHNZpCivadxOx77ync Cells expressing a catalytically-dead version of the enzyme, Top1.Y773F, also displayed resistance to peroxides (Supplementary Figure S1B), suggesting that lack of topoi- somerase activity is involved in this phenotype. ------- COMMENT: a64204abb2ff60b8 3 kWAU4+cpQ6azVRNMc8NF+8L50ss Regarding tolerance to oxidative stress, cells lacking Hrp3 or Snf22 are severely sensitive to oxidative stress (Figure 6A), ------- COMMENT: a64204abb2ff60b8 4 kWAU4+cpQ6azVRNMc8NF+8L50ss Regarding tolerance to oxidative stress, cells lacking Hrp3 or Snf22 are severely sensitive to oxidative stress (Figure 6A), ------- COMMENT: a64204abb2ff60b8 5 SxlbrzRXAw4i8H8qh3YHjgl7krA On the contrary, deletion of hrp1 yielded a strain resis- tant to H2 O2 , almost to the same extent as top1 (Fig- ure 6B). ------- COMMENT: a64204abb2ff60b8 6 GWYYUpdvi6XEzcjkzNmX3xwvV3Y and the combination of gene deletions of top1 with either hrp3 or snf22 yielded strains with intermediate pheno- types, suggesting that Top1 and these chromatin remodelers affect oxidative stress tolerance through independent mecha- nisms. (Figure 6A) ------- COMMENT: a64204abb2ff60b8 7 14XBl8jUp1Vhbbk0VM+Gqhwyzs0 The resistance phenotypes of the single deletes did not add in the double deletion strain, suggesting that Top1 and Hrp1 contribute to H2O2 tolerance through a similar mechanism. ------- COMMENT: a64204abb2ff60b8 8 GWYYUpdvi6XEzcjkzNmX3xwvV3Y and the combination of gene deletions of top1 with either hrp3 or snf22 yielded strains with intermediate pheno- types, suggesting that Top1 and these chromatin remodelers affect oxidative stress tolerance through independent mecha- nisms. (Figure 6A) ------- COMMENT: a64204abb2ff60b8 9 4ZVy6d9t/Byj5AbQFe+KASh8uco 15 min after stress imposition, only few nucle- osomes were still partially evicted in a wild-type background, whereas the number of nucleosomes which remained signifi- cantly less occupied relative to untreated conditions was much higher in cells lacking Top1 (Figure 4D and E; Supplementary Table S5). Of note, the differences observed for the averaged nucleosome maps of the 494 stress genes between wild-type and top1 strains was more dramatic for the highly expressed genes of the quartil 1, than for the genes of the other quartils (unpublished data). ------- COMMENT: a64204abb2ff60b8 10 rBDa9c2Q9xLBVGitrSOyaRnWCo0 In fact, the Top1 deficient strains displayed resistance to camptothecin, as expected (Supplementary Figure S1C): ------- COMMENT: a64204abb2ff60b8 11 rjlClKt0xZz4C8iM6iMkE/ErN9w We determined by northern blot that the ctt1, srx1 and hsp9 genes, coding for the anti-stress proteins catalase, sul- firedoxin and the chaperone Hsp9, respectively, were up- regulated in top1 cells to a larger extent that in a wild- type strain upon H2O2 stress (Figure 1B and Supplemen- tary Figure S1E); ------- COMMENT: a64204abb2ff60b8 12 rjlClKt0xZz4C8iM6iMkE/ErN9w We determined by northern blot that the ctt1, srx1 and hsp9 genes, coding for the anti-stress proteins catalase, sul- firedoxin and the chaperone Hsp9, respectively, were up- regulated in top1 cells to a larger extent that in a wild- type strain upon H2O2 stress (Figure 1B and Supplemen- tary Figure S1E); ------- COMMENT: a64204abb2ff60b8 13 rjlClKt0xZz4C8iM6iMkE/ErN9w We determined by northern blot that the ctt1, srx1 and hsp9 genes, coding for the anti-stress proteins catalase, sul- firedoxin and the chaperone Hsp9, respectively, were up- regulated in top1 cells to a larger extent that in a wild- type strain upon H2O2 stress (Figure 1B and Supplemen- tary Figure S1E); ------- COMMENT: a64204abb2ff60b8 14 7kr0f3b6KTLoMIa7s9datAoRsKU Never- theless, we showed by western blot that Atf1 phosphorylation was not exacerbated in cells lacking Top1 (Figure 1C). ------- COMMENT: a64204abb2ff60b8 15 q7GSv8bR8oANjVug4gEcEd2FPIU Simi- larly, we determined by ChIP that Atf1 recruitment to stress promoters was unaltered in top1 cells (Figure 1D). ------- COMMENT: a64204abb2ff60b8 16 v+5O6TAUoWFScGHeTb63VyLy5Og As shown in Figure 2A, Top1-HA accumulated at ORFs of the ctt1 and gpd1 genes after peroxide stress; ------- COMMENT: a64204abb2ff60b8 17 XqXa+xTpddyNwGokEZmu8jZ9+1c In cells lacking Top1, Pol II recruitment was more sustained than in wild-type cells (compare 60 min in both backgrounds in Figure 2B). ------- COMMENT: a64204abb2ff60b8 21 aQGgWWi/N6wHAt2j/b9if6jcpgk ells lacking Snf22 and Hrp3 were largely unable to open the chromatin structure at the ctt1 gene upon stress imposition. ------- COMMENT: a64204abb2ff60b8 22 aQGgWWi/N6wHAt2j/b9if6jcpgk ells lacking Snf22 and Hrp3 were largely unable to open the chromatin structure at the ctt1 gene upon stress imposition. ------- COMMENT: a64204abb2ff60b8 23 R5jzyjF/OFLNHU8xybA7SKOhKxc As shown in Supplementary Figure S7F, the stress-dependent recruitment of Ser2-phosphorylated Pol II to the ctt1 gene was significantly higher in cells lacking Hrp1 than in wild-type cells. ------- COMMENT: a64fc9a5b004423d 10 +f2NJC6Dk1/K3VquWSXu6TYBrMI (comment: Val: moved down from FYPO:0001429, its a fully penetrant inviable phenotype (anucleate)) ------- COMMENT: a64fc9a5b004423d 46 Iq2ukMXodAITFeZNn92PMgd5iGM (comment: CHECK cdc18delta::p[nmt*.cdc18+-LEU2]) ------- COMMENT: a64fc9a5b004423d 53 Iq2ukMXodAITFeZNn92PMgd5iGM (comment: CHECK cdc18delta::p[nmt*.cdc18+-LEU2]) ------- COMMENT: a6782fab5fd233d0 1 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a6782fab5fd233d0 2 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a6782fab5fd233d0 3 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a6782fab5fd233d0 4 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a6782fab5fd233d0 5 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a6782fab5fd233d0 6 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a6782fab5fd233d0 7 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a6782fab5fd233d0 8 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a6782fab5fd233d0 9 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: a6782fab5fd233d0 10 jBiuDADX2Oztel3zu/B6GlPRwCw Fig. 1, Fig. 8 ------- COMMENT: a6782fab5fd233d0 11 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: a6782fab5fd233d0 12 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: a6782fab5fd233d0 13 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: a6782fab5fd233d0 14 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: a6782fab5fd233d0 15 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a6782fab5fd233d0 16 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a6782fab5fd233d0 17 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a6782fab5fd233d0 18 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a6782fab5fd233d0 19 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a6782fab5fd233d0 20 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a6782fab5fd233d0 21 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: a6782fab5fd233d0 22 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: a6782fab5fd233d0 23 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: a6782fab5fd233d0 24 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: a6782fab5fd233d0 26 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: a6782fab5fd233d0 27 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a6782fab5fd233d0 28 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a6782fab5fd233d0 29 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a6782fab5fd233d0 30 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a6782fab5fd233d0 31 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a6782fab5fd233d0 32 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a6782fab5fd233d0 33 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a6782fab5fd233d0 34 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a6782fab5fd233d0 35 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a6782fab5fd233d0 36 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a6782fab5fd233d0 37 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: a6782fab5fd233d0 38 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: a6782fab5fd233d0 39 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: a6782fab5fd233d0 40 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: a6782fab5fd233d0 41 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: a6782fab5fd233d0 42 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: a6782fab5fd233d0 43 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: a6782fab5fd233d0 44 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: a6782fab5fd233d0 45 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: a6782fab5fd233d0 46 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: a6782fab5fd233d0 47 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: a6782fab5fd233d0 48 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: a6782fab5fd233d0 49 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: a6782fab5fd233d0 50 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: a6782fab5fd233d0 51 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: a6782fab5fd233d0 52 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: a6782fab5fd233d0 53 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: a6782fab5fd233d0 54 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: a6782fab5fd233d0 55 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: a6782fab5fd233d0 56 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: a6782fab5fd233d0 57 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: a6782fab5fd233d0 58 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: a6782fab5fd233d0 59 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: a6782fab5fd233d0 60 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: a6782fab5fd233d0 61 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: a6782fab5fd233d0 62 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: a6782fab5fd233d0 63 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: a6782fab5fd233d0 64 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: a6782fab5fd233d0 65 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: a67f3e355eafcf32 40 5+Kp4vg3nokc8ego4U8+EE8BIVY (comment: assayed with other MCM subunits present) ------- COMMENT: a6b360390b9f2e33 3 QTz8+fAbAlChgrEDPJIkOspK9js This result suggests that rings either slid off the center of the cell after assembly or assembled off center in ∆imp2 cells (Figure 1B). ------- COMMENT: a6b360390b9f2e33 7 Bp8wbF3QzpvnJExvNgbHO99kKPk (comment: Its localization to the ring is dependent on actin (LatA treatment).) ------- COMMENT: a6db3010aab2735f 2 w7nc2bpktlwMa32HJmnDGS1DMfo all microarray (table 1); arz1 also northern (fig 1) ------- COMMENT: a6db3010aab2735f 4 eA18qFqtgS1lrBo6FOMfXkfhp88 time course after transcription shutoff, so actually measuring degradation ------- COMMENT: a6e8deacb9e3b2bd 2 SBEke9EwwlJ+4ahdtsfX4Cv9SKA pkd2 mutants show temporary deflation followed by reinflation. pkd2-B42 has 50% lower spring constant as measured by Atomic Force Microscopy implicating reduced cellular stiffness. This indicates a reduced ability of this mutant at maintaining cellular turgor. ------- COMMENT: a6e8deacb9e3b2bd 3 UUKuYnqKkxVKBbdRKggXkE34Cqo (comment: CHECK Negative genetic interaction between ace::kanR and pkd2-B42) ------- COMMENT: a6e8deacb9e3b2bd 4 TqkaHRxd5B8Ck0SEDN75Nd87oBY (comment: CHECK Negative genetic interaction between orb6-25 and pkd2-B42.) ------- COMMENT: a6e8deacb9e3b2bd 6 qwb/Xk/0iwPRSvA+RrfTnEIWExY (comment: CHECK Negative genetic interaction mor2-282 and pkd2-B42.) ------- COMMENT: a6e8deacb9e3b2bd 7 QmX0F/B8PVP8xDWp8Viuowi1I+M (comment: CHECK Negative genetic interaction between cdc16-116 and pkd2-B42.) ------- COMMENT: a6e8deacb9e3b2bd 8 vkxtcbHwWQuZukHvq8I8rCcEri0 (comment: CHECK Positive genetic interaction between spg1-106 and pkd2-B42, spg1-106 and pkd2-81KD. Rescue in cell viability, septation, and cell lysis at restrictive temperature.) ------- COMMENT: a6e8deacb9e3b2bd 9 yYet4lHCAc1FNCI5MwkiA8csfVU (comment: CHECK Positive genetic interaction between cdc14-118 and pkd2-81KD. Rescue in cell viability, septation, and cell lysis at restrictive temperature.) ------- COMMENT: a6e8deacb9e3b2bd 10 0iL9kKb2Z/qJaxhXojRKDXmPDJU (comment: CHECK Positive genetic interaction between sid4-A1 and pkd2-81KD. Rescue in cell viability, septation, and cell lysis at restrictive temperature.) ------- COMMENT: a6e8deacb9e3b2bd 11 eazoAP0pAak5GNC/4nVpkA1wv4A (comment: CHECK Positive genetic interaction between sid2-250 and pkd2-B42. Rescue in cell viability, septation, and cell lysis at restrictive temperature.) ------- COMMENT: a6e8deacb9e3b2bd 12 ZSFkAod6jRQX1tWBdQxfVJtOtf8 (comment: CHECK Positive genetic interaction between mob1-R4 and pkd2-B42. Rescue in cell viability, septation, and cell lysis at restrictive temperature.) ------- COMMENT: a6e8deacb9e3b2bd 13 m0XJzl/7M9XDeQo/tXluFNEAdJU The growth rate of pkd2-B42 at the restrictive temperature of 36C or higher is 80% lower than wild-type cells. ------- COMMENT: a6e8deacb9e3b2bd 15 Crs4qojb/Gtc9+4GW1NvzovoU0c Increased percentage of septated cells at both permissive and restrictive temperature. ------- COMMENT: a6e8deacb9e3b2bd 16 F8VkU1lkIyeUd7GN08v46fdCKEc pkd2-B42 rescued in septation and cell lysis in cdc11-123 at restrictive temperature. ------- COMMENT: a6e8deacb9e3b2bd 17 orCJ7V70K8kSNgMgF+Hbtxt6SkQ pkd2-B42 prevented cell lysis in cdc7-24 at restrictive temperature. ------- COMMENT: a6edcf7d02f0562a 18 Yv3uPtsHDSTlF1Qu6YuTzdfh78o (comment: CHECK only in vitro data evidence) ------- COMMENT: a701986324b116dd 11 k4mpTT6NU5MWiZtYjzs7mw++xOY Our study revealed that Pho7, a TF activated by phosphate starvation80,81, specifically interacts with Rad24 and Rad25 also under stringent conditions (Figure 6A). ------- COMMENT: a701986324b116dd 27 k4mpTT6NU5MWiZtYjzs7mw++xOY Our study revealed that Pho7, a TF activated by phosphate starvation80,81, specifically interacts with Rad24 and Rad25 also under stringent conditions (Figure 6A). ------- COMMENT: a701986324b116dd 43 kX6B9d0s6QOR0reW66ztwhSR4QA Although histones were abundantly pulled down in all low stringency IPs, including the untagged control, this interaction was uniquely preserved for Pap1 and the heterodimeric TFs Atf1 and Pcr1 amongst TFs investigated under high stringency conditions (Figure 5C, Figure S5C) ------- COMMENT: a701986324b116dd 44 kX6B9d0s6QOR0reW66ztwhSR4QA Although histones were abundantly pulled down in all low stringency IPs, including the untagged control, this interaction was uniquely preserved for Pap1 and the heterodimeric TFs Atf1 and Pcr1 amongst TFs investigated under high stringency conditions (Figure 5C, Figure S5C) ------- COMMENT: a701986324b116dd 45 kX6B9d0s6QOR0reW66ztwhSR4QA Although histones were abundantly pulled down in all low stringency IPs, including the untagged control, this interaction was uniquely preserved for Pap1 and the heterodimeric TFs Atf1 and Pcr1 amongst TFs investigated under high stringency conditions (Figure 5C, Figure S5C) ------- COMMENT: a701986324b116dd 46 8slKpIBMuuh1ucTlUHatGPGfzIc Notably, Rad24 is known to negatively regulate Pho7- dependent pho1+ expression, with its deletion resulting in increased pho1+ levels even under phosphate-replete conditions82–84. Mechanistically, Rad24 has been linked to the regulation of an upstream long non-coding RNA (lncRNA), which is known to interfere with pho1+ expression in the absence of stress84,85. Our data suggests an alternative mechanism where Rad24, and potentially Rad25, directly interact with and negatively regulate Pho7, which would explain the de-repression of pho1+ in rad24Δ mutants. We identified two optimal 14-3-3 binding motifs78 in Pho7, corresponding to phosphorylated serine and threonine residues (RVCSAP (pS230) and RSFTNP (pT463))86–89 (Figure 6B). These motifs flank the Pho7 DBD, indicating that Rad24/Rad25 interactions could interfere with Pho7 DNA binding, similar to the mechanism proposed for the mammalian TF FOXO4 ------- COMMENT: a701986324b116dd 47 8slKpIBMuuh1ucTlUHatGPGfzIc Notably, Rad24 is known to negatively regulate Pho7- dependent pho1+ expression, with its deletion resulting in increased pho1+ levels even under phosphate-replete conditions82–84. Mechanistically, Rad24 has been linked to the regulation of an upstream long non-coding RNA (lncRNA), which is known to interfere with pho1+ expression in the absence of stress84,85. Our data suggests an alternative mechanism where Rad24, and potentially Rad25, directly interact with and negatively regulate Pho7, which would explain the de-repression of pho1+ in rad24Δ mutants. We identified two optimal 14-3-3 binding motifs78 in Pho7, corresponding to phosphorylated serine and threonine residues (RVCSAP (pS230) and RSFTNP (pT463))86–89 (Figure 6B). These motifs flank the Pho7 DBD, indicating that Rad24/Rad25 interactions could interfere with Pho7 DNA binding, similar to the mechanism proposed for the mammalian TF FOXO4 ------- COMMENT: a701986324b116dd 48 PrlZLDjRA3QJVPUy1YWKD6nHbVE (Figure 7) (comment: CHECK decreased localization of chromatin region to nuclear periphery) ------- COMMENT: a701986324b116dd 49 PrlZLDjRA3QJVPUy1YWKD6nHbVE (Figure 7) (comment: CHECK decreased localization of chromatin region to nuclear periphery) ------- COMMENT: a73f39d811302361 5 aGqBgDwQGf5/T1CKWPMC91jF7ss (comment: Scp1-13xMyc used for Scp1, pulled on Myc) ------- COMMENT: a73f39d811302361 6 KKuLtokTVtb/xIIqFks1p49sfsM (comment: pulled on Dsc2) ------- COMMENT: a73f39d811302361 8 NdkFjwFIzlgHhUU7HcwCBO2Am+8 Anp1-GFP mislocalized from Golgi puncta to ER and vacuole ------- COMMENT: a73f39d811302361 9 QWQRyPda2RB6Y7FlbLRmQ70RoZw Ost1-mCherry increased signal in ER ------- COMMENT: a73f39d811302361 10 gFJ4likRg+hqPm06RkVHPnqElQA Anp1-GFP degradation as assayed by appearance of free GFP ------- COMMENT: a73f39d811302361 11 70w6XCW6p8jlkAryXRNbGQDxPYE (comment: Ost1-mCherry, mCherry antibody) ------- COMMENT: a73f39d811302361 12 3LA1SfC2uJxNIr93PgpYe6dBfdM improved relative to WT Sre1 ------- COMMENT: a7499c82e9c96202 24 qh/CBcHEkB4zHn+WU3rxazsORcs inferred from Chk1 phosphorylation phenotypes ------- COMMENT: a75022bdef0034ab 1 bdgb8lN+JU9LBZmaPW8QUBFEOb0 During our investigation, we noticed that contractile ring shedding appeared prema- ture and exaggerated in ∆ain1 ∆myo51 cells when compared to wild-type cells. In wild- type cells expressing mEGFP-Myo2p, shedding begins when the ring is 62% constricted (n = 38 cells), and the fragments that shed from the constricting contractile ring extend out along the septum without reaching the full width of the cell (Figures 2A and 3A–C) ------- COMMENT: a75022bdef0034ab 2 e98ZqgJKrdbTD5kUYc9YBXK2Ito Figure 3. Contractile ring shedding is exaggerated and premature in ∆ain1 ∆myo51 cells. ------- COMMENT: a75022bdef0034ab 3 rPEbEAVEYiQJp197uoHROz0K6ts We acquired timelapse micrographs of ∆ain1 ∆myp2 cells expressing mEGFP-Myo2p to measure the duration of clumping when the onset of constriction is delayed. Forty-four percent of ∆ain1 ∆myp2 cells (n = 87 cells) showed the ring clumping phenotype, similar to ∆ain1 cells (Figure 1D). ------- COMMENT: a75022bdef0034ab 4 oqF4i8tKpFpUyoDUPpIksPnCRQw We measured the duration of clumps in ∆ain1 ∆myp2 cells and found that as expected, clumps last ~7 min longer in those cells (Figure 1E). ------- COMMENT: a75022bdef0034ab 5 e2Mk9K/ntuWd5RLnA5OJw0hsG3M node clumping, Myo2p marker figure 1A-D "The clumping phenotype suggests that nodes aggregate into clumps during contractile ring assembly in the absence of Ain1p." ------- COMMENT: a75022bdef0034ab 6 9FFsy8UDXq230azNf6SOMpa7Zk0 The onset of contractile ring constriction is delayed by ~10 min in ∆ain1 ∆myp2 cells. ------- COMMENT: a75022bdef0034ab 8 qLrgRyqObPaKfPRtrMgpl+RtOKk 3.4. Myo51 Localizes to the Inner Layer of the Contractile Ring during Constriction ------- COMMENT: a75a0c4b091bc54c 1 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: a75a0c4b091bc54c 2 joUktSPUGXl1ezDJB/dL25jTXFA figure1 ------- COMMENT: a75a0c4b091bc54c 3 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: a75a0c4b091bc54c 4 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: a75a0c4b091bc54c 5 BmoEvPinjQM3EhP3chwhidwDGsk Figure 3E) ------- COMMENT: a75a0c4b091bc54c 6 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: a75a0c4b091bc54c 7 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: a75a0c4b091bc54c 8 W6sX+zmHLLhu1V3t5LyrlkdfALk Fig. 3F ------- COMMENT: a75a0c4b091bc54c 10 BmoEvPinjQM3EhP3chwhidwDGsk Figure 3E) ------- COMMENT: a75a0c4b091bc54c 11 bQrAvw5v3pdeGEjTasNZSNQl2dw Figure 6A ------- COMMENT: a75a0c4b091bc54c 14 3y+clQlI+5kHCBL69R+N9citgBs (comment: CHECK cdc3-124, cdc15-delta2) ------- COMMENT: a75a0c4b091bc54c 15 hzALW1QLLml8QTRUggK/+WqWct4 (comment: CHECK cdc12-112, cdc15-delta2) ------- COMMENT: a75a0c4b091bc54c 16 IgCT1hgUwHzIqCia2sbLoQvMsKg (comment: CHECK cdc12-deltaC, cdc15-delta2) ------- COMMENT: a75a0c4b091bc54c 17 JtwR8HsT/x9Q4U/u/RSFBMgkiCs (comment: CHECK cdc15-delta2, fim1delta) ------- COMMENT: a75a0c4b091bc54c 18 oClnWiBNJxk2m26DHWn6YPZNnlI (comment: CHECK cdc16-116, cdc15-delta2) ------- COMMENT: a75a0c4b091bc54c 19 ftvIwrrX06JxkQUOTiQdp8C0wvs (comment: CHECK acp1delta, cdc15-delta2) ------- COMMENT: a75a0c4b091bc54c 20 tRKpxEk88MG5WDM1n9kt+c5zwic (comment: CHECK adf1-1, cdc15-delta2) ------- COMMENT: a75a0c4b091bc54c 21 6WdT31AHQjJs6sPP7oPhqCXf2KM (comment: CHECK ain1delta, cdc15-delta2) ------- COMMENT: a75a0c4b091bc54c 22 DrjrQXXtVU/T4YgvYXXArKO65D0 (comment: CHECK cdc12-4A, cdc15-delta2) ------- COMMENT: a75a0c4b091bc54c 23 jnMsOtbY/cbhoXnlLJRo8cqqmOk (comment: CHECK cps1-191, cdc15-delta2) ------- COMMENT: a75a0c4b091bc54c 24 s6WRKkAgvEOPfqReCF3Qkb4NJnQ (comment: CHECK efr3delta, cdc15-delta2) ------- COMMENT: a75a0c4b091bc54c 25 JfPqavk3psUEKTwNQq+e4J7Ai+I (comment: CHECK mid1delta, cdc15-delta2) ------- COMMENT: a75a0c4b091bc54c 26 SB65syOrDIQW/0QMDkq1XscA1h8 (comment: CHECK rlc1delta, cdc15-delta2) ------- COMMENT: a75a0c4b091bc54c 27 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: a75a0c4b091bc54c 28 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: a75a0c4b091bc54c 29 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: a75a0c4b091bc54c 30 ojJJQRPlmDWxZr90gIK5li1+arc figS3C ------- COMMENT: a75a0c4b091bc54c 31 ojJJQRPlmDWxZr90gIK5li1+arc figS3C ------- COMMENT: a75a0c4b091bc54c 32 ojJJQRPlmDWxZr90gIK5li1+arc figS3C ------- COMMENT: a75a0c4b091bc54c 33 ojJJQRPlmDWxZr90gIK5li1+arc figS3C ------- COMMENT: a75a0c4b091bc54c 34 abADctV53L9UQ2gjwODg3UcGTZc (comment: synonym =ring collapse) fig3F ------- COMMENT: a75a0c4b091bc54c 35 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: a765c0082a3d7619 1 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: a765c0082a3d7619 2 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: a765c0082a3d7619 3 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: a765c0082a3d7619 4 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: a765c0082a3d7619 5 b+fnRmdCMsF3f2HrTUzHdVvdItI Fig. 2A and C ------- COMMENT: a765c0082a3d7619 6 b+fnRmdCMsF3f2HrTUzHdVvdItI Fig. 2A and C ------- COMMENT: a765c0082a3d7619 7 b+fnRmdCMsF3f2HrTUzHdVvdItI Fig. 2A and C ------- COMMENT: a765c0082a3d7619 8 b+fnRmdCMsF3f2HrTUzHdVvdItI Fig. 2A and C ------- COMMENT: a765c0082a3d7619 9 N3sK+92PJ+HMkOBTwl3b5bW4WFo Fig. 2B and C ------- COMMENT: a765c0082a3d7619 10 N3sK+92PJ+HMkOBTwl3b5bW4WFo Fig. 2B and C ------- COMMENT: a765c0082a3d7619 11 N3sK+92PJ+HMkOBTwl3b5bW4WFo Fig. 2B and C ------- COMMENT: a765c0082a3d7619 12 N3sK+92PJ+HMkOBTwl3b5bW4WFo Fig. 2B and C ------- COMMENT: a765c0082a3d7619 13 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: a765c0082a3d7619 14 /jW72RtcYFWfOvrTHnBDcBe+Jyg Fig. 2, Fig. 3 ------- COMMENT: a765c0082a3d7619 15 /jW72RtcYFWfOvrTHnBDcBe+Jyg Fig. 2, Fig. 3 ------- COMMENT: a765c0082a3d7619 16 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: a765c0082a3d7619 17 nRGvjHkOUgQ2BW1CQOCHCA4qZPw Fig. 5A, B and C ------- COMMENT: a765c0082a3d7619 18 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: a765c0082a3d7619 19 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: a765c0082a3d7619 20 3cShD/iRi+Qd85UQ3quSnSIAZE4 These results furthered the notion that the function of Mid1p and corti- cal nodes was important for organization of normal actomy- osin rings in early mitosis. ------- COMMENT: a78bfbf7b3a5462a 10 COH5wJN8X/KWopaLm2DBstItAbQ (comment: CHECK occurs_at CSL_response_element, overexpression, in vitro) ------- COMMENT: a78bfbf7b3a5462a 18 FW56b2NQYdmcZMf9dMPs3hkUn+A (comment: CHECK to CSL_response_element) ------- COMMENT: a78bfbf7b3a5462a 21 FW56b2NQYdmcZMf9dMPs3hkUn+A (comment: CHECK to CSL_response_element) ------- COMMENT: a78bfbf7b3a5462a 23 /pDqDvHvvZ4ctgL/szCtytFKJF4 (comment: CHECK overexpression) ------- COMMENT: a78bfbf7b3a5462a 24 XM60262LjxTkLQkERyn+cTjsHpI (comment: CHECK occurs_at CSL_response_element in vivo) ------- COMMENT: a78bfbf7b3a5462a 28 mru/aT9hdnht0TOXXRfzAV8n/CM major region affecting localization between aa 395–465 ------- COMMENT: a7a3831a50c4517f 1 gAp20wxQtP7qTR+kW6K9CRjamyw Figure 3E-F, Supplementary figure 8 C-D ------- COMMENT: a7a3831a50c4517f 5 0Rl+m/x2je2GmSdaS61U6NDy30w Figure 3C-D, Supplementary Figure 8A-B ------- COMMENT: a7a3831a50c4517f 6 ttcEatvHZ12shpUw+vOJbVFNKyc (comment: Histone H3 amino terminus peptide) ------- COMMENT: a7a3831a50c4517f 7 c+yDTEr1q4Xk4DVgJ0qzXCe4HPk (comment: cnp1 amino terminus peptide) ------- COMMENT: a7a3831a50c4517f 8 gJxlJbK8EYaEFPIaNqmx+/Pyw6k (comment: cnp1 animo terminus peptide) ------- COMMENT: a7a3831a50c4517f 9 ttcEatvHZ12shpUw+vOJbVFNKyc (comment: Histone H3 amino terminus peptide) ------- COMMENT: a7a3831a50c4517f 13 qeObzl3cmqlruD6FJJBFEUp9X3A reduced interaction to cnp1 4PA peptide ------- COMMENT: a7a3831a50c4517f 14 AhG1TGcVJXg4Ca3iACtEaeOSIxQ Figure 2A, B, C ------- COMMENT: a7a3831a50c4517f 15 cefbuX43QSu8ptaTmu1cQGj+FyA Figure 2E-F, supplementary figure 5 ------- COMMENT: a7a3831a50c4517f 16 aixUC+/hX68MLNzYVne2mB68lYQ Figure 2G, supplementary figure 4 ------- COMMENT: a7a3831a50c4517f 17 c+yDTEr1q4Xk4DVgJ0qzXCe4HPk (comment: cnp1 amino terminus peptide) ------- COMMENT: a7a3831a50c4517f 18 wRqyqshugQrMWzIVhZFjUD0/o48 Figure S1A and B ------- COMMENT: a7a3831a50c4517f 20 wRqyqshugQrMWzIVhZFjUD0/o48 Figure S1A and B ------- COMMENT: a7a3831a50c4517f 21 wRqyqshugQrMWzIVhZFjUD0/o48 Figure S1A and B ------- COMMENT: a7a3831a50c4517f 22 wRqyqshugQrMWzIVhZFjUD0/o48 Figure S1A and B ------- COMMENT: a7a3831a50c4517f 23 5WQO3MjgVrp1+sQ45E8QjGjz5VM fig 1b, C ------- COMMENT: a7a3831a50c4517f 24 giS+uoL5FbLg9Wb3UIV8CCXSQ2w fig 1b, C, 3H ------- COMMENT: a7a3831a50c4517f 25 giS+uoL5FbLg9Wb3UIV8CCXSQ2w fig 1b, C, 3H ------- COMMENT: a7a3831a50c4517f 26 5WQO3MjgVrp1+sQ45E8QjGjz5VM fig 1b, C ------- COMMENT: a7a3831a50c4517f 27 5WQO3MjgVrp1+sQ45E8QjGjz5VM fig 1b, C ------- COMMENT: a7a3831a50c4517f 28 5WQO3MjgVrp1+sQ45E8QjGjz5VM fig 1b, C ------- COMMENT: a7a3831a50c4517f 29 5WQO3MjgVrp1+sQ45E8QjGjz5VM fig 1b, C ------- COMMENT: a7a3831a50c4517f 30 5WQO3MjgVrp1+sQ45E8QjGjz5VM fig 1b, C ------- COMMENT: a7a3831a50c4517f 31 5WQO3MjgVrp1+sQ45E8QjGjz5VM fig 1b, C ------- COMMENT: a7a3831a50c4517f 32 J0aqpbBvwP2S79icCGoEZimYMvY fig 4a ------- COMMENT: a7a3831a50c4517f 33 J0aqpbBvwP2S79icCGoEZimYMvY fig 4a ------- COMMENT: a7a3831a50c4517f 34 O+ogVpvOU5jD1UOIYSW5k9q1UZE fig 4b ------- COMMENT: a7a3831a50c4517f 35 O+ogVpvOU5jD1UOIYSW5k9q1UZE fig 4b ------- COMMENT: a7a3831a50c4517f 37 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: a7ac6f2d54760132 3 GMxhApamc2rFN7cwgJRSD07lPxo Figures 4B and 4C: Asp56Ser mutation endows Mag2 with the ability to excise εA at levels similar to Mag1. ------- COMMENT: a7c6118865e72409 25 xDZEpzPy+ji8VbgllRY2ZVf7wGQ (comment: CHECK positive regulation) ------- COMMENT: a7c6118865e72409 33 5IRcxemc1CYF658GXmHzjpmj+Qo (comment: All at eng2 CDS) ------- COMMENT: a7c6118865e72409 35 5IRcxemc1CYF658GXmHzjpmj+Qo (comment: All at eng2 CDS) ------- COMMENT: a7c6118865e72409 37 5IRcxemc1CYF658GXmHzjpmj+Qo (comment: All at eng2 CDS) ------- COMMENT: a7c6118865e72409 45 kqRQ8sgBvgtb8AsJJIzfpOFzrJU (comment: Mcs6 "primes" Rpb1 for phosphorylation by cdk9) ------- COMMENT: a820cd9476653a92 1 4kU2A2GJiG/O6td/XpEy21d1+M4 Figure 1a ------- COMMENT: a820cd9476653a92 2 4kU2A2GJiG/O6td/XpEy21d1+M4 Figure 1a ------- COMMENT: a820cd9476653a92 3 4kU2A2GJiG/O6td/XpEy21d1+M4 Figure 1a ------- COMMENT: a820cd9476653a92 4 4kU2A2GJiG/O6td/XpEy21d1+M4 Figure 1a ------- COMMENT: a820cd9476653a92 5 4kU2A2GJiG/O6td/XpEy21d1+M4 Figure 1a ------- COMMENT: a820cd9476653a92 6 4kU2A2GJiG/O6td/XpEy21d1+M4 Figure 1a ------- COMMENT: a820cd9476653a92 7 4kU2A2GJiG/O6td/XpEy21d1+M4 Figure 1a ------- COMMENT: a820cd9476653a92 8 4kU2A2GJiG/O6td/XpEy21d1+M4 Figure 1a ------- COMMENT: a820cd9476653a92 9 4kU2A2GJiG/O6td/XpEy21d1+M4 Figure 1a ------- COMMENT: a820cd9476653a92 10 4kU2A2GJiG/O6td/XpEy21d1+M4 Figure 1a ------- COMMENT: a820cd9476653a92 11 4kU2A2GJiG/O6td/XpEy21d1+M4 Figure 1a ------- COMMENT: a820cd9476653a92 12 4kU2A2GJiG/O6td/XpEy21d1+M4 Figure 1a ------- COMMENT: a820cd9476653a92 13 4kU2A2GJiG/O6td/XpEy21d1+M4 Figure 1a ------- COMMENT: a820cd9476653a92 14 4kU2A2GJiG/O6td/XpEy21d1+M4 Figure 1a ------- COMMENT: a820cd9476653a92 17 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: a820cd9476653a92 18 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: a820cd9476653a92 22 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: a820cd9476653a92 25 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: a820cd9476653a92 26 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: a820cd9476653a92 27 g6Jn2em/8AAfBsk05ufIaGmFks4 Fig 5J ------- COMMENT: a820cd9476653a92 28 wA2zDi2ljZyRdZxkVyg7LW8joIY Fig 5C ------- COMMENT: a820cd9476653a92 29 tebnVSgQVP8XiXJno5vZQcOggfM Fig 5G ------- COMMENT: a820d20db389b57f 1 JoZrOEaTyzHc2x9XQw/hb6w7LWA Analyzed by the GFP-tagged Cnp1 and RFP-tagged sfi1. ------- COMMENT: a820d20db389b57f 2 2uRkxuwyh0+Q/iELkk499HUb4ZI Analyzed by the GFP-visualized telomere-adjacent sod2 locus and RFP-tagged sfi1. ------- COMMENT: a820d20db389b57f 3 2uRkxuwyh0+Q/iELkk499HUb4ZI Analyzed by the GFP-visualized telomere-adjacent sod2 locus and RFP-tagged sfi1. ------- COMMENT: a820d20db389b57f 4 bLJuWeoQUtBjtyQjSFEwHTv/Rhs Analyzed by the GFP-tagged Cnp1 and RFP-tagged Sfi1. ------- COMMENT: a820d20db389b57f 5 U/5P+2JmTsyUI82VIReEhbvC2uc Enhanced telomere clustering defects ------- COMMENT: a820d20db389b57f 6 2uRkxuwyh0+Q/iELkk499HUb4ZI Analyzed by the GFP-visualized telomere-adjacent sod2 locus and RFP-tagged sfi1. ------- COMMENT: a820d20db389b57f 7 9qziQdm/wUJTtHZzNsNqP7N1ZDc Analyzed by GFP-tagged Cnp1 and RFP-tagged Sfi1. ------- COMMENT: a820d20db389b57f 8 GEHxVBuqA29wq/Rt7tJXw77JmS4 Analyzed by the GFP-visualized telomere-adjacent sod2 locus and RFP-tagged Sfi1. ------- COMMENT: a820d20db389b57f 9 bLJuWeoQUtBjtyQjSFEwHTv/Rhs Analyzed by the GFP-tagged Cnp1 and RFP-tagged Sfi1. ------- COMMENT: a820d20db389b57f 10 E7q44jxZK2JCEDqnUQIXE4l+mQg Examined by GFP-tagged Nuf2. ------- COMMENT: a820d20db389b57f 11 E7q44jxZK2JCEDqnUQIXE4l+mQg Examined by GFP-tagged Nuf2. ------- COMMENT: a820d20db389b57f 12 E7q44jxZK2JCEDqnUQIXE4l+mQg Examined by GFP-tagged Nuf2. ------- COMMENT: a820d20db389b57f 13 E7q44jxZK2JCEDqnUQIXE4l+mQg Examined by GFP-tagged Nuf2. ------- COMMENT: a820d20db389b57f 14 n2iwRTbAZwOdm4p+rszed2ZmczY (comment: CHECK Increased equational segregation of sister chromatids at meiosis I.) ------- COMMENT: a820d20db389b57f 15 n2iwRTbAZwOdm4p+rszed2ZmczY (comment: CHECK Increased equational segregation of sister chromatids at meiosis I.) ------- COMMENT: a8245a213e3baa92 3 sT989sDxiVqv9mbz3MHVS2l3Tog figure 1C ------- COMMENT: a8245a213e3baa92 4 sT989sDxiVqv9mbz3MHVS2l3Tog figure 1C ------- COMMENT: a8245a213e3baa92 5 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: a8245a213e3baa92 6 BrfIbYRWSQXRJuEBq4H5V6IodbY fig 2B ------- COMMENT: a8245a213e3baa92 7 BrfIbYRWSQXRJuEBq4H5V6IodbY fig 2B ------- COMMENT: a8245a213e3baa92 8 UGGSCnJv7jwxDqan8mqZK1eU4c0 (comment: vw: in vitro purification system) ------- COMMENT: a8245a213e3baa92 10 UGGSCnJv7jwxDqan8mqZK1eU4c0 (comment: vw: in vitro purification system) ------- COMMENT: a8245a213e3baa92 14 UGGSCnJv7jwxDqan8mqZK1eU4c0 (comment: vw: in vitro purification system) ------- COMMENT: a82c265e16b26ca6 11 A4CgLmzWlq3ONgexXA5g7VCFOlQ Figure 1B-E and supplementary Figure S1B ------- COMMENT: a82c265e16b26ca6 12 A4CgLmzWlq3ONgexXA5g7VCFOlQ Figure 1B-E and supplementary Figure S1B ------- COMMENT: a82c265e16b26ca6 14 A4CgLmzWlq3ONgexXA5g7VCFOlQ Figure 1B-E and supplementary Figure S1B ------- COMMENT: a82c265e16b26ca6 15 A4CgLmzWlq3ONgexXA5g7VCFOlQ Figure 1B-E and supplementary Figure S1B ------- COMMENT: a82c265e16b26ca6 16 A4CgLmzWlq3ONgexXA5g7VCFOlQ Figure 1B-E and supplementary Figure S1B ------- COMMENT: a82c265e16b26ca6 17 A4CgLmzWlq3ONgexXA5g7VCFOlQ Figure 1B-E and supplementary Figure S1B ------- COMMENT: a82c265e16b26ca6 18 A4CgLmzWlq3ONgexXA5g7VCFOlQ Figure 1B-E and supplementary Figure S1B ------- COMMENT: a82c265e16b26ca6 19 /YjgqxTXk0IGbMcXyFsAA8D/074 Figure 2E ------- COMMENT: a82c265e16b26ca6 20 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: a82c265e16b26ca6 21 Vz0tMA7K1B1EgrCznnKHGYD4pFI that the amount of Tas3 but not Ago1 was significantly reduced once the hsp90-G84C cells were shifted to restrictive temperature of 37 °C for 4 hours (Figure 4B) ------- COMMENT: a82c265e16b26ca6 22 fEPPdd8vcIyAT29IadgAwvAhaOE (Figure 4B) ------- COMMENT: a82c265e16b26ca6 23 EAEOvWNDLvl5r8z+WuG2eVGjq78 We then examined how the altered protein level of Tas3 affects the RITS complex formation in vivo. Notably, the low level of Tas3 in hsp90-G84C cells could only recruit minimal amount of Ago1 detected by co-immunoprecipitation assay at elevated temperatures (Figure 4C). (Figure 4B) ------- COMMENT: a82c265e16b26ca6 24 qMI5HPtwZER84ki1aXbaJOJts9M even at 25 °C, Arb1-associated Ago1 in the immunoprecipitates from hsp90-G84C cells was much reduced compared to wild-type samples (Figure 4E), ------- COMMENT: a82c265e16b26ca6 25 azuHdVIj39sJNxkn4xiGaVUJPVk It is noteworthy that the amount of Arb1 was also drastically decreased in hsp90-G84C cells at high temperatures (Figure 4E) ------- COMMENT: a82c265e16b26ca6 30 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: a82c265e16b26ca6 31 pZSJQXvoT1cUExD0UaNqQ5922mo fig 5e ------- COMMENT: a82c265e16b26ca6 32 W8U2HOh5c9FHrXYcx0iyvAr6hdQ Figure 7A ------- COMMENT: a8575ebe13d91e58 1 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: a8575ebe13d91e58 2 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: a8575ebe13d91e58 4 xwiAzWDZWsCZpLWWa+VhMiX8+fA Fig. 1B, D, E ------- COMMENT: a8575ebe13d91e58 5 xwiAzWDZWsCZpLWWa+VhMiX8+fA Fig. 1B, D, E ------- COMMENT: a8575ebe13d91e58 6 Y9v+BsNfj7JNJPyS2FxEXGjSDaA Fig. G ------- COMMENT: a8575ebe13d91e58 7 Y9v+BsNfj7JNJPyS2FxEXGjSDaA Fig. G ------- COMMENT: a8575ebe13d91e58 10 Koiej3/prPDgqBzIqkh+uTMstzA Fig. 2, F and G ------- COMMENT: a8575ebe13d91e58 11 MMr6EfkF+VFg2EgvQF6E4KCnS6Y Fig. 2B and C, Fig. 2D and E ------- COMMENT: a8575ebe13d91e58 12 GwDTRgjscvZCptfKwsmXogyTjVM Fig. 3A–D ------- COMMENT: a8575ebe13d91e58 13 GwDTRgjscvZCptfKwsmXogyTjVM Fig. 3A–D ------- COMMENT: a8575ebe13d91e58 14 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: a8575ebe13d91e58 15 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: a8575ebe13d91e58 20 7cbi9wm0cwYeupDHHOWWCgupQJ4 Fig. 4A and B ------- COMMENT: a8575ebe13d91e58 21 PdwhseqlrW5Xuya8UXCRybLS6G0 (comment: DNS) ------- COMMENT: a8575ebe13d91e58 22 PdwhseqlrW5Xuya8UXCRybLS6G0 (comment: DNS) ------- COMMENT: a8575ebe13d91e58 23 lVvMgkFNoEp4aURnzoIpcy7J//0 fig 5A ------- COMMENT: a8575ebe13d91e58 24 lVvMgkFNoEp4aURnzoIpcy7J//0 fig 5A ------- COMMENT: a8575ebe13d91e58 25 ID9jod+CYoeNtHU/1/pNRi2CyBo Fig. 5C and D ------- COMMENT: a8575ebe13d91e58 26 ID9jod+CYoeNtHU/1/pNRi2CyBo Fig. 5C and D ------- COMMENT: a882e140c77212d8 1 Ykwg70ahd1Pkt4qH4lJEBBrGj9k (comment: dependent on sme2 expression) ------- COMMENT: a8928db520ace5a9 2 UMI9MNz2LWtwZ7xh3nI7ssOLx6U Table S1, Figure S1 ------- COMMENT: a8928db520ace5a9 3 UMI9MNz2LWtwZ7xh3nI7ssOLx6U Table S1, Figure S1 ------- COMMENT: a8928db520ace5a9 4 UMI9MNz2LWtwZ7xh3nI7ssOLx6U Table S1, Figure S1 ------- COMMENT: a8928db520ace5a9 5 UMI9MNz2LWtwZ7xh3nI7ssOLx6U Table S1, Figure S1 ------- COMMENT: a8928db520ace5a9 6 UMI9MNz2LWtwZ7xh3nI7ssOLx6U Table S1, Figure S1 ------- COMMENT: a8928db520ace5a9 7 UMI9MNz2LWtwZ7xh3nI7ssOLx6U Table S1, Figure S1 ------- COMMENT: a8928db520ace5a9 8 UMI9MNz2LWtwZ7xh3nI7ssOLx6U Table S1, Figure S1 ------- COMMENT: a8928db520ace5a9 9 UMI9MNz2LWtwZ7xh3nI7ssOLx6U Table S1, Figure S1 ------- COMMENT: a8928db520ace5a9 10 UMI9MNz2LWtwZ7xh3nI7ssOLx6U Table S1, Figure S1 ------- COMMENT: a8928db520ace5a9 11 UMI9MNz2LWtwZ7xh3nI7ssOLx6U Table S1, Figure S1 ------- COMMENT: a8928db520ace5a9 12 UMI9MNz2LWtwZ7xh3nI7ssOLx6U Table S1, Figure S1 ------- COMMENT: a8928db520ace5a9 13 +r4pyuTBerkXRnSJTgxFrBqERTM Fig. 1c ------- COMMENT: a8928db520ace5a9 14 EaJ1cIJCgxH1h7dmT6mEA3YTf4A Fig. 1d ------- COMMENT: a8928db520ace5a9 15 ernVuLQ3ugzbI6GXr4Wb6RsgMe4 Fig. 2A ------- COMMENT: a8928db520ace5a9 16 Y/rK+VW4RXs7JXT+KWmX2SxPkAs figure 2A ------- COMMENT: a8928db520ace5a9 17 EaJ1cIJCgxH1h7dmT6mEA3YTf4A Fig. 1d ------- COMMENT: a8928db520ace5a9 18 ernVuLQ3ugzbI6GXr4Wb6RsgMe4 Fig. 2A ------- COMMENT: a8928db520ace5a9 19 ernVuLQ3ugzbI6GXr4Wb6RsgMe4 Fig. 2A ------- COMMENT: a8928db520ace5a9 20 ernVuLQ3ugzbI6GXr4Wb6RsgMe4 Fig. 2A ------- COMMENT: a8928db520ace5a9 21 ernVuLQ3ugzbI6GXr4Wb6RsgMe4 Fig. 2A ------- COMMENT: a8928db520ace5a9 22 ernVuLQ3ugzbI6GXr4Wb6RsgMe4 Fig. 2A ------- COMMENT: a8928db520ace5a9 23 ernVuLQ3ugzbI6GXr4Wb6RsgMe4 Fig. 2A ------- COMMENT: a8928db520ace5a9 25 DRYJSGGCd2yYeqANMtUVaEW/nts Fig. 3a ------- COMMENT: a8928db520ace5a9 26 r/R8vI6TkLOmQr47F7QOhGGegy4 Fig. 3b ------- COMMENT: a8928db520ace5a9 28 b6IAA5jq+UeIwjjz7zZu5qhGt80 Fig. 4b ------- COMMENT: a8928db520ace5a9 29 b6IAA5jq+UeIwjjz7zZu5qhGt80 Fig. 4b ------- COMMENT: a8928db520ace5a9 30 b6IAA5jq+UeIwjjz7zZu5qhGt80 Fig. 4b ------- COMMENT: a8928db520ace5a9 31 b6IAA5jq+UeIwjjz7zZu5qhGt80 Fig. 4b ------- COMMENT: a8928db520ace5a9 32 b6IAA5jq+UeIwjjz7zZu5qhGt80 Fig. 4b ------- COMMENT: a8928db520ace5a9 33 b6IAA5jq+UeIwjjz7zZu5qhGt80 Fig. 4b ------- COMMENT: a8928db520ace5a9 34 b6IAA5jq+UeIwjjz7zZu5qhGt80 Fig. 4b ------- COMMENT: a8928db520ace5a9 36 b6IAA5jq+UeIwjjz7zZu5qhGt80 Fig. 4b ------- COMMENT: a8928db520ace5a9 37 b6IAA5jq+UeIwjjz7zZu5qhGt80 Fig. 4b ------- COMMENT: a8928db520ace5a9 38 b6IAA5jq+UeIwjjz7zZu5qhGt80 Fig. 4b ------- COMMENT: a8a2700cbe528751 1 rR6ZV8K+XlKb4kl/8DRxwr7gEVQ fig 2e ------- COMMENT: a8a2700cbe528751 2 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: a8a2700cbe528751 3 GWgs9r9yLOQqn+7WFMPmzNTlqVM fig2e ------- COMMENT: a8a2700cbe528751 4 GWgs9r9yLOQqn+7WFMPmzNTlqVM fig2e ------- COMMENT: a8a2700cbe528751 5 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: a8a2700cbe528751 6 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: a8a2700cbe528751 7 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: a8a2700cbe528751 8 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: a8a2700cbe528751 14 J0aqpbBvwP2S79icCGoEZimYMvY fig 4a ------- COMMENT: a8a2700cbe528751 18 J0aqpbBvwP2S79icCGoEZimYMvY fig 4a ------- COMMENT: a8bdbe7f398d38ff 29 qFzRyJdztD6nluNTJG/C6EJE2Ds snoRNAs with extended poly(A) tails accumulate in these foci ------- COMMENT: a8bdbe7f398d38ff 32 qFzRyJdztD6nluNTJG/C6EJE2Ds snoRNAs with extended poly(A) tails accumulate in these foci ------- COMMENT: a935efede4be6aa8 2 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: a935efede4be6aa8 3 kTw+naaUc4EjcfwumeRZFSTYwqU (comment: G2 block) ------- COMMENT: a935efede4be6aa8 4 ZA9r8tZVbJmR2jvOYDkLYWwjoa4 (comment: G1 block) ------- COMMENT: a93df4140a4b1e4c 1 fsdKaLSRVAgCcnOAw+T9e0gGw8M (comment: Primer extension analysis) ------- COMMENT: a93df4140a4b1e4c 2 fsdKaLSRVAgCcnOAw+T9e0gGw8M (comment: Primer extension analysis) ------- COMMENT: a93df4140a4b1e4c 3 U8Nwf4qGK/3S5d+3PwLLtrqdaFk (comment: Primer Extension Analysis) ------- COMMENT: a93df4140a4b1e4c 4 fsdKaLSRVAgCcnOAw+T9e0gGw8M (comment: Primer extension analysis) ------- COMMENT: a93df4140a4b1e4c 5 fsdKaLSRVAgCcnOAw+T9e0gGw8M (comment: Primer extension analysis) ------- COMMENT: a93df4140a4b1e4c 6 fsdKaLSRVAgCcnOAw+T9e0gGw8M (comment: Primer extension analysis) ------- COMMENT: a93df4140a4b1e4c 9 2AiHxnfLYpHMfamTdOj1FQ+bDjc (comment: Northern blot and primer extension analysis) ------- COMMENT: a93df4140a4b1e4c 10 2AiHxnfLYpHMfamTdOj1FQ+bDjc (comment: Northern blot and primer extension analysis) ------- COMMENT: a93df4140a4b1e4c 16 2AiHxnfLYpHMfamTdOj1FQ+bDjc (comment: Northern blot and primer extension analysis) ------- COMMENT: a93df4140a4b1e4c 17 2AiHxnfLYpHMfamTdOj1FQ+bDjc (comment: Northern blot and primer extension analysis) ------- COMMENT: a93df4140a4b1e4c 18 2AiHxnfLYpHMfamTdOj1FQ+bDjc (comment: Northern blot and primer extension analysis) ------- COMMENT: a93df4140a4b1e4c 19 fsdKaLSRVAgCcnOAw+T9e0gGw8M (comment: Primer extension analysis) ------- COMMENT: a93df4140a4b1e4c 20 fsdKaLSRVAgCcnOAw+T9e0gGw8M (comment: Primer extension analysis) ------- COMMENT: a93df4140a4b1e4c 21 fsdKaLSRVAgCcnOAw+T9e0gGw8M (comment: Primer extension analysis) ------- COMMENT: a93df4140a4b1e4c 23 eitMkGsewO1J3kXvN9cqP6igLi8 Fig. 8c ------- COMMENT: a93df4140a4b1e4c 39 Ws72I+iiPoPquhonP6JL8/zBjBI (comment: CHECK GONEW: negative regulation of cellular response to phosphate starvation) ------- COMMENT: a94ed2012c4ff5bb 1 cQ08BfXoJu3sUREw3k32CA52dyc Fig. 5a ------- COMMENT: a94ed2012c4ff5bb 2 B20pQkXpP1LOyLDaV7hCpvyku/k Fig. 5b (comment: CHECK however, it colocalizes with Sgo1 at centromeres during meiosis I) ------- COMMENT: a94ed2012c4ff5bb 3 q7yQ4AbC2bEj6KiCpphVWV06cCo (comment: dns) ------- COMMENT: a94ed2012c4ff5bb 4 4tH1lGGiQ5H4CuuWXlLkKijQCjs (Fig. 5b). ------- COMMENT: a94ed2012c4ff5bb 5 OjxxWBawnCzkSRSlQzcQHUdfOFM We found that, like sgo1D cells, par1D cells mostly lost centromeric Rec8 localization at this stage (Fig. 5d) ------- COMMENT: a94ed2012c4ff5bb 6 /a3fvrFZv8uWxK7lhnxjIEZ8G6A (Supplementary Fig. 7)...both of these mutant cell types showed precocious centromeric dissociation after meiosis I, and random chromosome segregation following meiosis II ------- COMMENT: a94ed2012c4ff5bb 7 KThklxZ/dhL2o2T9wyna6N0CXF4 (Supplementary Fig. 7) ------- COMMENT: a94ed2012c4ff5bb 8 KThklxZ/dhL2o2T9wyna6N0CXF4 (Supplementary Fig. 7) ------- COMMENT: a94ed2012c4ff5bb 9 w53WTYFucZ/N/+LqKZHfoAG0qb4 (Supplementary Fig. 7). ------- COMMENT: a94ed2012c4ff5bb 11 cQ08BfXoJu3sUREw3k32CA52dyc Fig. 5a ------- COMMENT: a97bb48a3280a5fb 4 ygUku9uNwpUCYm44eVey2b776i0 ternary complex normally forms with Swi1-Swi3 and Mrc1 on DNA ------- COMMENT: a97bb48a3280a5fb 9 ygUku9uNwpUCYm44eVey2b776i0 ternary complex normally forms with Swi1-Swi3 and Mrc1 on DNA ------- COMMENT: a97bb48a3280a5fb 10 ygUku9uNwpUCYm44eVey2b776i0 ternary complex normally forms with Swi1-Swi3 and Mrc1 on DNA ------- COMMENT: a97bb48a3280a5fb 23 ygUku9uNwpUCYm44eVey2b776i0 ternary complex normally forms with Swi1-Swi3 and Mrc1 on DNA ------- COMMENT: a983b01258f7c025 1 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: a983b01258f7c025 2 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: a983b01258f7c025 3 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: a983b01258f7c025 4 JyKnxZeJ5mGBsqVFTMpU7nIJMXo Figure6 ------- COMMENT: a983b01258f7c025 5 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: a983b01258f7c025 6 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: a983b01258f7c025 7 wB7RJgwAySckrXRTm08TbE/Nmgw Figure 4 and 6 ------- COMMENT: a983b01258f7c025 8 wB7RJgwAySckrXRTm08TbE/Nmgw Figure 4 and 6 ------- COMMENT: a983b01258f7c025 9 wB7RJgwAySckrXRTm08TbE/Nmgw Figure 4 and 6 ------- COMMENT: a983b01258f7c025 10 AVbx7pOndTw+O9ruDb52p7r/HUQ Figure1 ------- COMMENT: a983b01258f7c025 11 AVbx7pOndTw+O9ruDb52p7r/HUQ Figure1 ------- COMMENT: a983b01258f7c025 14 z70v4aFd2oXCFGxGzvOXKeVfRVo (comment: Increased 4-fold,) Figure2 ------- COMMENT: a983b01258f7c025 15 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: a983b01258f7c025 16 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: a983b01258f7c025 19 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: a983b01258f7c025 20 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: a983b01258f7c025 21 b2vhogXuuKubNwqXc2ydCjT2H2s (comment: Increased 4-fold,) FigureS1 ------- COMMENT: a983b01258f7c025 22 z70v4aFd2oXCFGxGzvOXKeVfRVo (comment: Increased 4-fold,) Figure2 ------- COMMENT: a983b01258f7c025 23 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: a983b01258f7c025 24 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: a983b01258f7c025 25 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: a983b01258f7c025 26 E/3bB2Omqw0Fyq3PFCewRXf53YA Figure 3, (comment: assayed using Myo52) ------- COMMENT: a983b01258f7c025 27 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: a983b01258f7c025 28 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: a983b01258f7c025 29 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: a983b01258f7c025 30 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: a983b01258f7c025 31 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: a983b01258f7c025 32 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: a983b01258f7c025 33 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: a983b01258f7c025 34 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: a983b01258f7c025 35 VsXVAcjhhK3i/vXBjMbPn8JvElQ Figure5 ------- COMMENT: a983b01258f7c025 36 VsXVAcjhhK3i/vXBjMbPn8JvElQ Figure5 ------- COMMENT: a983b01258f7c025 37 wB7RJgwAySckrXRTm08TbE/Nmgw Figure 4 and 6 ------- COMMENT: a983b01258f7c025 38 NlBt2O1/ObVSAeuackYanIZ/biQ Figure 5,(comment: assayed using Myo52) ------- COMMENT: a983b01258f7c025 39 NlBt2O1/ObVSAeuackYanIZ/biQ Figure 5, (comment: assayed using Myo52) ------- COMMENT: a983b01258f7c025 40 NlBt2O1/ObVSAeuackYanIZ/biQ Figure 5, (comment: assayed using Myo52) ------- COMMENT: a983b01258f7c025 41 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: a983b01258f7c025 42 E/3bB2Omqw0Fyq3PFCewRXf53YA Figure 3, (comment: assayed using Myo52) ------- COMMENT: a983b01258f7c025 43 8QslXKqun9a6keC0Iz3W6AKbz3k (comment: assayed using CHD,) FigureS1 ------- COMMENT: a983b01258f7c025 44 3U7Ds/upuNQ1iaMS2E5URrRWqzQ FigureS3 ------- COMMENT: a983b01258f7c025 45 3U7Ds/upuNQ1iaMS2E5URrRWqzQ FigureS3 ------- COMMENT: a983b01258f7c025 46 3U7Ds/upuNQ1iaMS2E5URrRWqzQ FigureS3 ------- COMMENT: a983b01258f7c025 47 3U7Ds/upuNQ1iaMS2E5URrRWqzQ FigureS3 ------- COMMENT: a983b01258f7c025 48 qiPZj5rOsrsfVj40avFjt8flDLM Figure S5,(comment: assayed using LifeAct) ------- COMMENT: a983b01258f7c025 49 +3gvD2ljPpucTUkIR3anjwvLf10 Figure3, S4, (comment: assayed using Myo52 and Fus1) ------- COMMENT: a983b01258f7c025 51 UEf5BieiBBDtiZ6wSgPzy4Z5sQk Figure S1, (comment: assayed using CHD) ------- COMMENT: a983b01258f7c025 52 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: a983b01258f7c025 53 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: a983b01258f7c025 54 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: a983b01258f7c025 55 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: a983b01258f7c025 56 YEWIUG74AxfchIGAYvEoVOVkCsk (comment: 1.1 fold,) Figure S2 ------- COMMENT: a983b01258f7c025 57 xHgWiQGxcgBO0xJRn3WgCICGeX4 Figure S6 ------- COMMENT: a983b01258f7c025 58 xHgWiQGxcgBO0xJRn3WgCICGeX4 Figure S6 ------- COMMENT: a983b01258f7c025 59 xHgWiQGxcgBO0xJRn3WgCICGeX4 Figure S6 ------- COMMENT: a983b01258f7c025 60 xHgWiQGxcgBO0xJRn3WgCICGeX4 Figure S6 ------- COMMENT: a983b01258f7c025 61 3hjSuY088tlwNgfIqDAjChFpVFU FigureS5, (comment: assayed using LifeAct) ------- COMMENT: a983b01258f7c025 62 3hjSuY088tlwNgfIqDAjChFpVFU FigureS5, (comment: assayed using LifeAct) ------- COMMENT: a983b01258f7c025 63 W3QJm7ThqNyXmGP/YjtFjkWWtlk Figure2, (comment: Increased 1.5-fold, assayed using CHD) ------- COMMENT: a983b01258f7c025 64 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: a983b01258f7c025 65 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: a983b01258f7c025 66 JyKnxZeJ5mGBsqVFTMpU7nIJMXo Figure6 ------- COMMENT: a983b01258f7c025 67 JyKnxZeJ5mGBsqVFTMpU7nIJMXo Figure6 ------- COMMENT: a983b01258f7c025 68 JyKnxZeJ5mGBsqVFTMpU7nIJMXo Figure6 ------- COMMENT: a983b01258f7c025 69 JyKnxZeJ5mGBsqVFTMpU7nIJMXo Figure6 ------- COMMENT: a9ee2fb1cb19f385 1 41FlThg6fq1bXShZ7NxILAyIZbY (comment: vw edited) ------- COMMENT: a9ee2fb1cb19f385 4 5jjsmsRBt5SBDlNslNw8ziZFu90 RNA level increases upon amitrole exposure in wild type but not mutant ------- COMMENT: a9ee2fb1cb19f385 6 5jjsmsRBt5SBDlNslNw8ziZFu90 RNA level increases upon amitrole exposure in wild type but not mutant ------- COMMENT: a9ee2fb1cb19f385 47 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment: same as either single mutant) ------- COMMENT: a9ee2fb1cb19f385 48 gD2NmEDv2+zGyqUCUHAC2lX7Cmk (comment: same as cpc2delta alone) ------- COMMENT: a9ee2fb1cb19f385 49 gD2NmEDv2+zGyqUCUHAC2lX7Cmk (comment: same as cpc2delta alone) ------- COMMENT: a9ee2fb1cb19f385 50 K/KPnAFKxgb5TI6QtfPhfq2f0DE (comment: same as gcn2delta alone) ------- COMMENT: a9ee2fb1cb19f385 51 j7OjaUOpnnylPSWwBwQk31Heh7g (comment: worse than cpc2delta alone) ------- COMMENT: aa6c8a119000c53e 1 IKnDxLSWkMb1vuS7L/zUB5t6KQ8 (comment: control, functional fragment) ------- COMMENT: aa6c8a119000c53e 2 yq7YaBn7y7Sm69ITWJI9KPtf8sc Thus, Cdc12(FH1FH2)p can replace the essential functions of Cdc12p in vivo when Cdc12p is nonfunctional, but is toxic when overexpressed in the pres- ence of functional Cdc12p. ------- COMMENT: aa6c8a119000c53e 4 0dYnAmqcweXGJWee2Fx/e6bsq2Y figure 1D impressive enrichment of actin filaments in ab- errant thick cables and aster-like accumulations ------- COMMENT: aa6c8a119000c53e 5 0dYnAmqcweXGJWee2Fx/e6bsq2Y figure 1D impressive enrichment of actin filaments in ab- errant thick cables and aster-like accumulations ------- COMMENT: aa6c8a119000c53e 6 CtMXlUbqAzGNa+YzqV+Az/RpZdw figure 1D but lacked both actin con- tractile rings and polarized actin patches (Fig. 1 D) ------- COMMENT: aa6c8a119000c53e 7 CtMXlUbqAzGNa+YzqV+Az/RpZdw figure 1D but lacked both actin con- tractile rings and polarized actin patches (Fig. 1 D) ------- COMMENT: aa6c8a119000c53e 8 h7j8IAC+mPr+O47PBtWZKAeoqDs figure 1D arrested after 􏰖24 h (Fig. 1 E) ------- COMMENT: aa6c8a119000c53e 9 YGLPs0/XNVyyVu5mcPmbYhQVR/s (comment: CHECK abnormal (partial, broad, and misoriented) septa) (Fig. 1 G) ------- COMMENT: aa6c8a119000c53e 11 6fWiVurhbfxbWJzTYtrL0nDNK3E (comment: barbed end actin capping) ------- COMMENT: aa6c8a119000c53e 12 w3Nbs5CHbPyjgBQhxCPn8EfP90I (comment: MF?) ------- COMMENT: aa6c8a119000c53e 13 V5Tb8YdR+G3YDN/m7D9uraNfiYY fission yeast Cdc12 (FH1FH2)p purified from bacteria (Fig. 1 B) stimulated ac- tin polymerization, as detailed below (see Fig. 4). This is consistent with Cdc12(FH1FH2)p and MmCPcapping the barbed (fast depolymerizing) ends of the fila- ments with high affinity (Kd 􏰃 0.1 􏰂M), allowing dissocia- tion only from the slowly depolymerizing (Pollard, 1986) pointed ends (Caldwell et al., 1989) ------- COMMENT: aa6c8a119000c53e 16 FQdD8T69vhZZuqS2thftPBh+9XE (comment: actin binding inhibitor pointed end) ------- COMMENT: aa80b6544eead412 1 LANHoUXou/lrVPUA4dc267FAGjU (comment: endoglycosidase-H cleaves N-linked glycosylation) ------- COMMENT: aaac35de67e48eb3 1 KgL778IL8ENNH+U6tkd501VsFhI Experimental evidence in Fig. S7. ------- COMMENT: aaac35de67e48eb3 2 QtFkizXI9ZRHX0Y+GUFRem8zMYg Rend domain (683-847) receives mechanical force signal. It buffers force by unfolding when force is larger than 15 pN and refolding when force drops below 2 pN. ------- COMMENT: aaac35de67e48eb3 3 qUqP5cyxm6YcTAhz05Hph5gvu9M (comment: test) ------- COMMENT: aaac35de67e48eb3 4 qUqP5cyxm6YcTAhz05Hph5gvu9M (comment: test) ------- COMMENT: aaac35de67e48eb3 5 McDH+CSUIZql0VWh9DU61V0Tv1s Rend domain (Rod domain of END4): mechanosensitive five-helix bundle that unfolds over 15 pN, refolds under 2 pN; when unfolded, condensates End4 at sites of clathrin-mediated endocytosis ------- COMMENT: aabe6ef236eda9f5 3 r+2aaXLpLL3uDO1MXGQzLL8TvgQ fig S2D ------- COMMENT: aabe6ef236eda9f5 4 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: aabe6ef236eda9f5 5 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: aabe6ef236eda9f5 6 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: aabe6ef236eda9f5 7 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: aabe6ef236eda9f5 8 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: aabe6ef236eda9f5 9 yn5/s6S+P83NU/sZm1yJpcjeWbk fig 3D ------- COMMENT: aabe6ef236eda9f5 10 yn5/s6S+P83NU/sZm1yJpcjeWbk fig 3D ------- COMMENT: aabe6ef236eda9f5 11 QJKtBLGTusuUnyDiTJQzioGJDsE fig 3E ------- COMMENT: aabe6ef236eda9f5 12 QJKtBLGTusuUnyDiTJQzioGJDsE fig 3E ------- COMMENT: aabe6ef236eda9f5 17 UWOcy6qySzXZ9vKfEPX06/E3lR8 Fig. 5D (comment: a bit tenuous but we don't have this annotated..) ------- COMMENT: aabe6ef236eda9f5 18 UWOcy6qySzXZ9vKfEPX06/E3lR8 Fig. 5D (comment: a bit tenuous but we don't have this annotated..) ------- COMMENT: aabe6ef236eda9f5 24 kAjzk1ABsUcdCmmRtcM7j+Fg22s (comment: I replaced GO:0090579 dsDNA loop formation as per https://github.com/geneontology/go-annotation/issues/3610) ------- COMMENT: aac1ee2617f3c41b 1 TUl9fngQi9T2r1Ci1WAnQSqMOPg fig 1a ------- COMMENT: aac1ee2617f3c41b 2 TUl9fngQi9T2r1Ci1WAnQSqMOPg fig 1a ------- COMMENT: aac1ee2617f3c41b 3 SMJ+dJ0jvSMhIyIBGFXZrYZkDm0 fig 1b ------- COMMENT: aac1ee2617f3c41b 4 q13k6EZBmzjrx5icKY1aM/9DAt8 (comment: poly Ub,) fig1c ------- COMMENT: aac1ee2617f3c41b 20 WZQaGUi5yPkSMiu5Vop5Ig1+Sfo (comment: nuclear membrane) ------- COMMENT: aac1ee2617f3c41b 22 UEXjWzDsPEP5fOy0rwk/xLGHK8I (comment: proteasome) ------- COMMENT: aac1ee2617f3c41b 28 c9iC7BOonisl0YFfk/sRXoTAbrI (comment: CHECK short half life of cut8 depends on rhp6 dept ubiquitination) ------- COMMENT: aac1ee2617f3c41b 51 TJID2mC4jRljzfT7s2JSKKijA48 (comment: CHECK Ubr1 and Rhp18 Ligases Are Required for the Short Half-Life of Amino-Terminal Fragment) ------- COMMENT: aac1ee2617f3c41b 53 TJID2mC4jRljzfT7s2JSKKijA48 (comment: CHECK Ubr1 and Rhp18 Ligases Are Required for the Short Half-Life of Amino-Terminal Fragment) ------- COMMENT: aac2a8d125c42565 2 SLF6Fb8AlO8nD1S/3os64JFaJjE Figure 1E and Supplemental Figure S1C ------- COMMENT: aac2a8d125c42565 3 SLF6Fb8AlO8nD1S/3os64JFaJjE Figure 1E and Supplemental Figure S1C ------- COMMENT: aac2a8d125c42565 4 SLF6Fb8AlO8nD1S/3os64JFaJjE Figure 1E and Supplemental Figure S1C ------- COMMENT: aac2a8d125c42565 5 BFc5K1H1zk4tvjVujrcsU5IxPLs Figure 1C and Supplemental Figure S1C ------- COMMENT: aac2a8d125c42565 6 iAcbpl1ew+nFwb8AiAqOLZxpvN8 Figure 1D ------- COMMENT: aac2a8d125c42565 7 S8HXyeUsCSaorBAFM9hH7KFLRtQ (comment: complements deletion) ------- COMMENT: aac2a8d125c42565 8 S8HXyeUsCSaorBAFM9hH7KFLRtQ (comment: complements deletion) ------- COMMENT: aac2a8d125c42565 9 S8HXyeUsCSaorBAFM9hH7KFLRtQ (comment: complements deletion) ------- COMMENT: aac2a8d125c42565 10 S8HXyeUsCSaorBAFM9hH7KFLRtQ (comment: complements deletion) ------- COMMENT: aac2a8d125c42565 11 S8HXyeUsCSaorBAFM9hH7KFLRtQ (comment: complements deletion) ------- COMMENT: aac2a8d125c42565 12 S8HXyeUsCSaorBAFM9hH7KFLRtQ (comment: complements deletion) ------- COMMENT: aac2a8d125c42565 13 ozyhHtZvhbhzjjQFQ3hkfgotXGQ (comment: Kd ≈ 20 μM) Supplemental Figure S5B ------- COMMENT: aac2a8d125c42565 14 S8HXyeUsCSaorBAFM9hH7KFLRtQ (comment: complements deletion) ------- COMMENT: aac2a8d125c42565 15 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: aac2a8d125c42565 16 S8HXyeUsCSaorBAFM9hH7KFLRtQ (comment: complements deletion) ------- COMMENT: aac2a8d125c42565 17 5IGc3difXx4+SJNj9Vf3lGyEbhs Figure 4F ------- COMMENT: aac2a8d125c42565 18 oc5MEL5yp/a7PKINsg3J2flqOZk Figure 4F (comment: inferred penetrance because growth not m,uch affected) ------- COMMENT: aac2a8d125c42565 19 5IGc3difXx4+SJNj9Vf3lGyEbhs Figure 4F ------- COMMENT: aac2a8d125c42565 20 b+00gXuAJVmG6MoHxSub6s9WxVw Figure 4C ------- COMMENT: aac2a8d125c42565 21 b+00gXuAJVmG6MoHxSub6s9WxVw Figure 4C ------- COMMENT: aac2a8d125c42565 22 RI4VNbNTfW7lSyWZRZuArS5UdKQ (comment: complements deletion) Figure 6A ------- COMMENT: aac2a8d125c42565 23 RI4VNbNTfW7lSyWZRZuArS5UdKQ (comment: complements deletion) Figure 6A ------- COMMENT: aac2a8d125c42565 24 RI4VNbNTfW7lSyWZRZuArS5UdKQ (comment: complements deletion) Figure 6A ------- COMMENT: aac2a8d125c42565 25 RI4VNbNTfW7lSyWZRZuArS5UdKQ (comment: complements deletion) Figure 6A ------- COMMENT: aac2a8d125c42565 26 RI4VNbNTfW7lSyWZRZuArS5UdKQ (comment: complements deletion) Figure 6A ------- COMMENT: aac2a8d125c42565 30 MM6XHC+7e8HqWH308Z+psPBVz+s (comment: in interphase) ------- COMMENT: aac2a8d125c42565 33 9ihM2778M1CRHPoMIuTDS4njLVU Supple- mental Figure S9C) ------- COMMENT: aac2a8d125c42565 34 6eIbOIfqz+gsbbwVrK4+CBoNIQM (Figure 3). ------- COMMENT: aac2a8d125c42565 35 6eIbOIfqz+gsbbwVrK4+CBoNIQM (Figure 3). ------- COMMENT: aac2a8d125c42565 36 6eIbOIfqz+gsbbwVrK4+CBoNIQM (Figure 3). ------- COMMENT: ab023f5573fbf770 1 vntVqmz2uFwWB0esRouQEnZLUjc (comment: Temperature was shifted at anaphase B.) ------- COMMENT: ab023f5573fbf770 2 efKLk6V+B1DSwzHUfy/jeEIVr/U (comment: Temperature was shifted at prophase or metaphase.) ------- COMMENT: ab023f5573fbf770 3 5CwtP7YuGGdMvBlfOF6hI1IjeZs fig 1. ------- COMMENT: ab023f5573fbf770 4 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: ab023f5573fbf770 5 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: ab023f5573fbf770 6 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: ab023f5573fbf770 9 yCmdVJDtzOwNfVPxOl+QWvnifTU SFig5 ------- COMMENT: ab08277b7b3bc9c4 1 fYqQOpzikopc+3AWAEPEUkYII7w figure 4 ------- COMMENT: ab08277b7b3bc9c4 2 Zw9BumV5ekIs9EtO51SFzg5cj7E (comment: High temp - 30 degrees. (VW I changed this from a cell phenotype term to a population phenotype term) ) fig1 We interpret this suppression to indicate that the lethal mitoses, which occur in the smallest cells, require Cig2/CDK activity instead of, or in addi- tion to, Cdc13/CDK activity. ------- COMMENT: ab08277b7b3bc9c4 3 AteAC7zImI2hwjN3yqqToYNVcRI fig1, 2 (comment: Nick suggested "mitotic catastrophe"We would make this a related synonym?) ------- COMMENT: ab08277b7b3bc9c4 4 fNpmaE7MG19qjD0+PlLVAROJUuU fig 5a ------- COMMENT: ab08277b7b3bc9c4 5 xt3X3UoCDigio10KhQTAIaNEbA8 figure 6b ------- COMMENT: ab08277b7b3bc9c4 6 iRRwhkJObOW5ruvOaJe8Y8ojRTU fig2 (comment: CONDITION 30 degrees) wee1-50ts mik1D cells divide at a smaller size than wee1-50ts mik1D cig2D cells ------- COMMENT: ab08277b7b3bc9c4 9 2rGAWFu0JXTt1p5pNtLBQLqU0Iw fig3 (comment: i.e wee?) ------- COMMENT: ab08277b7b3bc9c4 10 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: ab08277b7b3bc9c4 11 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: ab08277b7b3bc9c4 12 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: ab08277b7b3bc9c4 13 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: ab08277b7b3bc9c4 14 NdJkRLzM11O37Xi3iYQRTIGW34c Fig 5b (comment: (I only curated the red line temp inc at tome zero, becase it would be difficult to specify at 60 mins, I could not think of a way to do this). I'm assuming these cells do not enter mitosis, is that correct) ------- COMMENT: ab08277b7b3bc9c4 16 MiKUzpxbTMApPIFioLvn5RWvEiY fig 6a ------- COMMENT: ab08277b7b3bc9c4 17 fYqQOpzikopc+3AWAEPEUkYII7w figure 4 ------- COMMENT: ab08277b7b3bc9c4 18 GIiSuLJi0GddtRHFJ8PNZjx4Vuc nmt81:cig2 constitutive expression suppresses the temperature-sensitive lethality of cdc13-G282Dts. ------- COMMENT: ab232c941560cd80 6 lBaAFXG9QaeOPbWle8fVxJxhhSU Hence, from these results it was evident that Hrp1, Hrp3 and Nap1 occupancy in vivo generally correlated with increased nucleosome densities in the corresponding mutants, and that this effect was most pronounced in promoter regions. ------- COMMENT: ab232c941560cd80 7 lBaAFXG9QaeOPbWle8fVxJxhhSU Hence, from these results it was evident that Hrp1, Hrp3 and Nap1 occupancy in vivo generally correlated with increased nucleosome densities in the corresponding mutants, and that this effect was most pronounced in promoter regions. ------- COMMENT: ab232c941560cd80 8 lBaAFXG9QaeOPbWle8fVxJxhhSU Hence, from these results it was evident that Hrp1, Hrp3 and Nap1 occupancy in vivo generally correlated with increased nucleosome densities in the corresponding mutants, and that this effect was most pronounced in promoter regions. ------- COMMENT: ab2c8f1287892790 60 EnNR2tViXXkHYwpDRDUwDKVLAdY (comment: CHECK during recovery from stress) ------- COMMENT: ab447353ab14b3a0 10 5aoM0prON+2u/9sTNmqZe8OQzb0 (comment: basal transcription is meaningless because emm contains calcium) ------- COMMENT: ab5171b90f5cef1d 1 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: ab5171b90f5cef1d 2 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: ab5171b90f5cef1d 3 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: ab5171b90f5cef1d 4 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: ab5171b90f5cef1d 5 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: ab5171b90f5cef1d 6 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: ab5171b90f5cef1d 7 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: ab5171b90f5cef1d 8 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: ab5171b90f5cef1d 9 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: ab5171b90f5cef1d 10 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: ab5171b90f5cef1d 11 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: ab5171b90f5cef1d 12 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: ab5171b90f5cef1d 13 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: ab5171b90f5cef1d 14 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: ab5171b90f5cef1d 15 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: ab5171b90f5cef1d 16 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: ab5171b90f5cef1d 17 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: ab5171b90f5cef1d 18 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: ab5171b90f5cef1d 19 mKHrmdoHpr4rPKS2pKRyXvNdG7s Fig. S6 ------- COMMENT: ab5171b90f5cef1d 20 mKHrmdoHpr4rPKS2pKRyXvNdG7s Fig. S6 ------- COMMENT: ab5171b90f5cef1d 21 mKHrmdoHpr4rPKS2pKRyXvNdG7s Fig. S6 ------- COMMENT: ab5171b90f5cef1d 22 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: ab5171b90f5cef1d 23 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: ab5171b90f5cef1d 24 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: ab5171b90f5cef1d 25 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: ab5171b90f5cef1d 26 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: ab5171b90f5cef1d 27 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: ab5171b90f5cef1d 34 7fbuic9J4nxECAcRt8dTP1kf/5Q Fig. S8 ------- COMMENT: ab5171b90f5cef1d 35 7fbuic9J4nxECAcRt8dTP1kf/5Q Fig. S8 ------- COMMENT: ab5171b90f5cef1d 36 wr5TXzzUQ5a8pjtOfW1cRWvVFaY Fig. 7D ------- COMMENT: ab5171b90f5cef1d 37 wr5TXzzUQ5a8pjtOfW1cRWvVFaY Fig. 7D ------- COMMENT: ab5171b90f5cef1d 38 wr5TXzzUQ5a8pjtOfW1cRWvVFaY Fig. 7D ------- COMMENT: ab7d71d62a2030e3 6 4zbnQ5N3OvTOuQQrYwwLzHGqKGE (comment: polysome profile) ------- COMMENT: ab7d71d62a2030e3 8 4zbnQ5N3OvTOuQQrYwwLzHGqKGE (comment: polysome profile) ------- COMMENT: ab801a175ed0c533 1 PxGYNeSxrV5PD+XotSQQNBxfnSo fig 1a ------- COMMENT: ab801a175ed0c533 2 HsNiYrwz2c6wTo8j6XG+/b1jEMQ S. pombe Cdk9 also generated Ser2-P and Ser5-P signals but was relatively inefficient at phosphorylating Ser7. ------- COMMENT: ab801a175ed0c533 3 Hv96GxGcfFeD0Si6kWvLIdSHk9E fig1 (comment: carboxy terminal region) ------- COMMENT: ab801a175ed0c533 4 PxGYNeSxrV5PD+XotSQQNBxfnSo fig 1a ------- COMMENT: ab801a175ed0c533 5 GRwcWiNYEoJvlEFNDOXYfUPbals fig 1a (comment: CHECK in vitro /in vivo) ------- COMMENT: ab801a175ed0c533 6 Hv96GxGcfFeD0Si6kWvLIdSHk9E fig1 (comment: carboxy terminal region) ------- COMMENT: ab801a175ed0c533 7 PxGYNeSxrV5PD+XotSQQNBxfnSo fig 1a ------- COMMENT: ab801a175ed0c533 8 QHYo8y6+voeOvRaD9HDjjb498xI fig 1e (comment: CHECK in vitro) ------- COMMENT: ab801a175ed0c533 9 iA5dY9YEw9Oz3oRtEdjLM4Hgje4 We also compared the activities of kinase complexes generated by translation in vitro toward Spt5. By this measurement also, Cdk9 and Cdk9􏰂C were stimulated to similar extents by Csk1 (Fig. 1F),(comment: is this an physiological substrate?) ------- COMMENT: ab801a175ed0c533 10 FJrUrY/cNbL7O7xtjP6w5yuBdIM fig 2b ------- COMMENT: ab801a175ed0c533 11 CpLPgm8i0a3op3IoshdhYxoBsbU fig 2c ------- COMMENT: ab801a175ed0c533 12 CpLPgm8i0a3op3IoshdhYxoBsbU fig 2c ------- COMMENT: ab801a175ed0c533 13 CpLPgm8i0a3op3IoshdhYxoBsbU fig 2c ------- COMMENT: ab801a175ed0c533 14 CpLPgm8i0a3op3IoshdhYxoBsbU fig 2c ------- COMMENT: ab801a175ed0c533 15 CpLPgm8i0a3op3IoshdhYxoBsbU fig 2c ------- COMMENT: ab801a175ed0c533 16 CpLPgm8i0a3op3IoshdhYxoBsbU fig 2c ------- COMMENT: ab801a175ed0c533 17 kF22OQpo78dl63iWtauu1nqaICc fig 2c MPA exacerbates growth impairment in mutants defective in transcript elongation (8, 11, 50), although the precise mechanism of this effect is un- known (34) ------- COMMENT: ab801a175ed0c533 18 CpLPgm8i0a3op3IoshdhYxoBsbU fig 2c ------- COMMENT: ab801a175ed0c533 19 CpLPgm8i0a3op3IoshdhYxoBsbU fig 2c ------- COMMENT: ab801a175ed0c533 20 HLZi5j47rU5wfgHVApLUWJUMGjM fig 2d (comment: CHECK Spt5-T1P (CTD repeat 1 residue)) ------- COMMENT: ab801a175ed0c533 21 HLZi5j47rU5wfgHVApLUWJUMGjM fig 2d (comment: CHECK Spt5-T1P (CTD repeat 1 residue)) ------- COMMENT: ab801a175ed0c533 22 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: ab801a175ed0c533 23 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: ab801a175ed0c533 25 c8NPwnUE9C1Wyo5NmK/u8nISuEw fig 4A, B ------- COMMENT: ab801a175ed0c533 26 c8NPwnUE9C1Wyo5NmK/u8nISuEw fig 4A, B ------- COMMENT: ab801a175ed0c533 27 7LbunRME4eqAFlNakkMGknrs0+Y . The preference of the conserved Cdk9 catalytic domain in fission yeast and metazoans for Ser7-modified CTD substrates, moreover, implies a conserved mechanism to impose order on the Pol II transcription cycle. ------- COMMENT: ab801a175ed0c533 28 DHLyh0YOfItltRxf+168kFxaHOI (Fig. 6C) Immunoblot analysis indicated that Mcs6 phosphorylates Ser2, Ser5, and Ser7 ------- COMMENT: ab801a175ed0c533 29 DHLyh0YOfItltRxf+168kFxaHOI (Fig. 6C) Immunoblot analysis indicated that Mcs6 phosphorylates Ser2, Ser5, and Ser7 ------- COMMENT: ab801a175ed0c533 30 DHLyh0YOfItltRxf+168kFxaHOI (Fig. 6C) Immunoblot analysis indicated that Mcs6 phosphorylates Ser2, Ser5, and Ser7 ------- COMMENT: ab801a175ed0c533 31 DHLyh0YOfItltRxf+168kFxaHOI (Fig. 6C) Immunoblot analysis indicated that Mcs6 phosphorylates Ser2, Ser5, and Ser7 ------- COMMENT: ab801a175ed0c533 32 HsNiYrwz2c6wTo8j6XG+/b1jEMQ S. pombe Cdk9 also generated Ser2-P and Ser5-P signals but was relatively inefficient at phosphorylating Ser7. ------- COMMENT: ab801a175ed0c533 33 HsNiYrwz2c6wTo8j6XG+/b1jEMQ S. pombe Cdk9 also generated Ser2-P and Ser5-P signals but was relatively inefficient at phosphorylating Ser7. ------- COMMENT: ab90f75fa22cff89 9 JIVcGzBXeSrO8iBVQb3AJN/WI2c (comment: has condensed chromosomes) ------- COMMENT: ab9f58e60ef0a260 1 5p9Do9oMY3QmD2M10tIewdtju+s The spotting assay revealed the enhanced pyrogallol sensitivity of wat1/pop3 delete cells as compared to wild type cells (Fig. 1A) ------- COMMENT: aba22b8a34948b96 1 ekDPQHbAnbe6qq1nVLc0Qsg/Ow0 Fig1A ------- COMMENT: aba22b8a34948b96 2 b/W/YC3L0U5kOg0yP2N2gsIXCDA Fig1C ------- COMMENT: aba22b8a34948b96 3 b/W/YC3L0U5kOg0yP2N2gsIXCDA Fig1C ------- COMMENT: aba22b8a34948b96 4 b/W/YC3L0U5kOg0yP2N2gsIXCDA Fig1C ------- COMMENT: aba22b8a34948b96 10 V/sqdJfARA3ERBq8q85r2vimXKU Fig2C the cdc2-cig2 and cdc2-cdc13 complexes have increased kinase activity ------- COMMENT: aba22b8a34948b96 12 Nukc+znT/Ak3NvrSqBlW0kPh2kA Fig2C the cdc2-cig2 and cdc2-cdc13 complexe have no tyrosine 15 phosphorylation ------- COMMENT: aba22b8a34948b96 14 /RDGMekTROyURN2SZW31BLEjkW4 Fig 4B cig2 over expression also occurs when cells blocked with HU ------- COMMENT: aba22b8a34948b96 15 HPW75PfVjX2I2Pi5ziCeNwHs7qs Fig 3A, D ------- COMMENT: aba22b8a34948b96 16 uxb4ExMdVIn4asod7PiBVeuEAeg Fig 3C (comment: cig2 over expression from ~10hours after thiamine removal) ------- COMMENT: aba22b8a34948b96 17 xLhtRN3hOjc+uKJocxGI1W2m5KU Fig 3B no G1 peak is observed showing that S phase onset is not delayed ------- COMMENT: aba22b8a34948b96 18 D6ifPAg1vGbqRn/ucCmUsat3K3U Fig5A small peak of less that 1C DNA content ------- COMMENT: aba22b8a34948b96 19 cHGDjSLTRKJdQRdU753evl54Yb0 Fig 3 data not shown cell viability is reduced at late time points ------- COMMENT: aba22b8a34948b96 20 O3lyv+byJacQta9anHIDA8HIMmI Fig5A used forward scatter to measure cell size small peak of short cells ------- COMMENT: aba22b8a34948b96 21 aeDz64nQzk/PIeNpi60y7Q1y7ZI Fig5A lower panel ------- COMMENT: aba22b8a34948b96 22 /5kYnadVgt1Fmp/ou7TuKzhTwCc Fig5A lower panel used forward scatter to measure cell size ------- COMMENT: aba22b8a34948b96 23 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: aba22b8a34948b96 26 lgsb6g5hGgtSH8SneqCBHVsZWHY Fig 4A cells block normally with 1C DNA content even when cig2 is over expressed ------- COMMENT: aba22b8a34948b96 27 0iAlVmkzj8c/9UdwHKdD/qqoKsc (comment: CHECK fypo/issues/3165) Fig5B in the absence of cig2 there is a delay in the appearance of cut cells ------- COMMENT: aba22b8a34948b96 28 REqA86wgEckE1+po1IZQ8wC5RUg Fig5C ------- COMMENT: aba22b8a34948b96 29 bYVgy/zyYY6/9ICqKfaJa2DVMhI (comment: CHECK fypo/issues/3165) Fig5C ------- COMMENT: aba22b8a34948b96 30 gYP9jiwN1IrYYHDDJpxlYSUPQJM Fig1D The protein cdc2 protein assayed is in complex with cig2 as there is no cdc2-cdc13 complex present ------- COMMENT: aba22b8a34948b96 31 3b3QSoBCKuA+mYiq+JcZzX6++1w Fig1D ------- COMMENT: abb52d9d2b18f1e0 4 a8kzl0vA/oONouX1BWa674UTzsA (comment: kinase assay, and hybridization with S. cerevisiae PKC) ------- COMMENT: abbc849e1c0c5544 13 JXI0vKZwNMIIFUrz/xOiNWELaqU (comment: over 35) ------- COMMENT: abbc849e1c0c5544 16 Ji/WV5CZagZXZjpDhSN41k/AJuw (comment: over 25) ------- COMMENT: abbc849e1c0c5544 17 Ji/WV5CZagZXZjpDhSN41k/AJuw (comment: over 25) ------- COMMENT: abbc849e1c0c5544 18 Ji/WV5CZagZXZjpDhSN41k/AJuw (comment: over 25) ------- COMMENT: abbc849e1c0c5544 19 Ji/WV5CZagZXZjpDhSN41k/AJuw (comment: over 25) ------- COMMENT: abefd0a167d6fbd7 4 TDFmDAxG4mr7tKjmU5DX2TEFPgc (comment: CHECK mhf1-L78R) ------- COMMENT: ac12e0d42489768e 2 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: ac12e0d42489768e 3 4R+lBpYtzgzk2vhxC1Wq1WZ0svw Fig8 ------- COMMENT: ac12e0d42489768e 4 4R+lBpYtzgzk2vhxC1Wq1WZ0svw Fig8 ------- COMMENT: ac29fd3d76564172 1 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: ac29fd3d76564172 2 Mc1Dl/8ePjG+W3BQIevC1vaEvqw Figure 2D ------- COMMENT: ac29fd3d76564172 3 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: ac29fd3d76564172 4 AhUTcPt2yf+eJdRcCiv/pw114ac Fig. 3B, Figure 4A and B, ------- COMMENT: ac29fd3d76564172 5 AhUTcPt2yf+eJdRcCiv/pw114ac Fig. 3B, Figure 4A and B, ------- COMMENT: ac29fd3d76564172 9 YGTHdFPWAmmR96d6Ldv0NPUQzEU Fig 3A ------- COMMENT: ac29fd3d76564172 10 YGTHdFPWAmmR96d6Ldv0NPUQzEU Fig 3A ------- COMMENT: ac29fd3d76564172 11 LKylDQ32oIRV5Cd84EhzZgc4BE4 Figure 4A and B, ------- COMMENT: ac29fd3d76564172 12 BprhfysZecSHnDRVGx+GjRUIYco fig 4D ------- COMMENT: ac29fd3d76564172 13 ERYIUtEmJTU+Azmsgm69M9t5hCM Figures 5D, 6C ------- COMMENT: ac29fd3d76564172 14 ERYIUtEmJTU+Azmsgm69M9t5hCM Figures 5D, 6C ------- COMMENT: ac29fd3d76564172 15 ERYIUtEmJTU+Azmsgm69M9t5hCM Figures 5D, 6C ------- COMMENT: ac29fd3d76564172 16 BprhfysZecSHnDRVGx+GjRUIYco fig 4D ------- COMMENT: ac29fd3d76564172 17 BprhfysZecSHnDRVGx+GjRUIYco fig 4D ------- COMMENT: ac29fd3d76564172 18 LKylDQ32oIRV5Cd84EhzZgc4BE4 Figure 4A and B, ------- COMMENT: ac29fd3d76564172 19 LKylDQ32oIRV5Cd84EhzZgc4BE4 Figure 4A and B, ------- COMMENT: ac29fd3d76564172 20 LKylDQ32oIRV5Cd84EhzZgc4BE4 Figure 4A and B, ------- COMMENT: ac29fd3d76564172 21 LKylDQ32oIRV5Cd84EhzZgc4BE4 Figure 4A and B, ------- COMMENT: ac98af394a19cfe7 15 vR4QHsxCJEwQST2EeYj9K2Gc4Ts figure2b ------- COMMENT: ac98af394a19cfe7 16 vR4QHsxCJEwQST2EeYj9K2Gc4Ts figure2b ------- COMMENT: ac98af394a19cfe7 17 vR4QHsxCJEwQST2EeYj9K2Gc4Ts figure2b ------- COMMENT: ac98af394a19cfe7 18 vR4QHsxCJEwQST2EeYj9K2Gc4Ts figure2b ------- COMMENT: acb154d034f85813 21 NxEt+GwkuOcBV8x7w1/QP0d4W68 (comment: not arrested like wee1-50 overexp alone) ------- COMMENT: acb154d034f85813 22 kB2a+eAteZeJ+iBIxDqQLs2oa1o (comment: not arrested like wee1+ overexp alone) ------- COMMENT: acb154d034f85813 23 NxEt+GwkuOcBV8x7w1/QP0d4W68 (comment: not arrested like wee1-50 overexp alone) ------- COMMENT: acb154d034f85813 24 UEjeGnyG2hd804phBRVC3+d7SXo (comment: higher than wee1 not overexp, but lower than wee1-50 overexp in wt bkg) ------- COMMENT: acd1a2d2ddcf29cc 1 BddHv3c1iiQO7DISXLf00llr4TA Cut12.V85A, Cut12G71VF87L, Cut12PDSA, Cut12.G71, Cut12PD swap mutants suppress cdc25-22 and allows growth at 36°C ------- COMMENT: acd1a2d2ddcf29cc 2 vG9EpCs6FPQSVtBpyfEB1AT/iKU Fig1D, E ------- COMMENT: acd1a2d2ddcf29cc 3 3b3QSoBCKuA+mYiq+JcZzX6++1w Fig1D ------- COMMENT: acd1a2d2ddcf29cc 4 3b3QSoBCKuA+mYiq+JcZzX6++1w Fig1D ------- COMMENT: acd1a2d2ddcf29cc 5 R3dx+QHZFLYK6Z1DnE59M93A7XY Fig1C increased interaction in 2 hybrid ------- COMMENT: acd1a2d2ddcf29cc 7 F4d7dQR1sD8hFRpA09ziMP5rR+8 Fig1E ------- COMMENT: acd1a2d2ddcf29cc 8 yquSVQOPpp3pXIjyxWokeAS7PQo Fig1E ------- COMMENT: acd1a2d2ddcf29cc 9 yquSVQOPpp3pXIjyxWokeAS7PQo Fig1E ------- COMMENT: acd1a2d2ddcf29cc 10 yquSVQOPpp3pXIjyxWokeAS7PQo Fig1E ------- COMMENT: acd1a2d2ddcf29cc 11 F4d7dQR1sD8hFRpA09ziMP5rR+8 Fig1E ------- COMMENT: acd1a2d2ddcf29cc 12 wqEcyRpv2qTVDDoBli17FqNx3j8 Fig1F reduced interaction between Dis2 and Cut12.F87L ------- COMMENT: acd1a2d2ddcf29cc 15 BBGUFTaV0e33l+HiMg53r1f0owE cut12.1 cdc25-22 only grows at 20°C ------- COMMENT: acd1a2d2ddcf29cc 16 Cfx7QAe2TE+ZCspYstaaTzggTcM Fig1A, 2A. vw"Fig 1. Rescue of cdc25-22 but not restored to full growth (partial rescue)" ------- COMMENT: acd1a2d2ddcf29cc 19 aCbHitDUhoB343UKVtNxUrLKrzU Fig2G ------- COMMENT: acd1a2d2ddcf29cc 20 fyAttHyLcx86WS5JOnosiswfhPg Fig2H ------- COMMENT: acd1a2d2ddcf29cc 21 +k8l3snHnODAbS2CQHag3m5zt9Y Fig 2E phospho mimetic cdc12 mutant rescues cdc25 mutant ------- COMMENT: acd1a2d2ddcf29cc 22 aWxzhCemKfw9SS1KBCrnz1NoDig Fig 2E unphosphorylatable cut12 mutants are unable to rescue cdc25 mutant (comment: vw changed from decreased to abolished?) ------- COMMENT: acd1a2d2ddcf29cc 23 hBw/gVXx5oOsXP+95MKidL2jsFo Fig2E single mutant T78D does not rescue cdc25-22 as well as double T75DT78D. (vw changed from increased to decreased as we are comparing to WT , bottom row) ------- COMMENT: acd1a2d2ddcf29cc 24 42p4yVULbSKvrW5HxO5h1/BDHnU Fig2E single mutant T75D does not rescue cdc25-22 as well as double T75DT78D or singleT78D mutants. (comment: vw: changed to decreased) ------- COMMENT: acd1a2d2ddcf29cc 25 haSW6TYgvtlc3dTm4N+CXS5fvSA Fig2I single mutant cut12. T75A binds dis2 ------- COMMENT: acd1a2d2ddcf29cc 26 Uq7edQvCJFOaibHDyX8l8KNzlvA Fig2I single mutant cut12.T78A binds Dis2 ------- COMMENT: acd1a2d2ddcf29cc 27 iY3qbJ3oNe+NEgQTsNM6/RymvTk Fig2I single mutant cut12. T75D reduces dis2 binding ------- COMMENT: acd1a2d2ddcf29cc 28 k2IC518czTi7/cUdOwPz0RzvOcM Fig2I single mutant cut12. T78D reduces dis2 binding ------- COMMENT: acd1a2d2ddcf29cc 29 6ATH+YmlhJE3SNGLMwW8FPunHp4 Fig2I Fig3C double mutant cut12.T75A T78A binds dis2 ------- COMMENT: acd1a2d2ddcf29cc 32 MJg/5WefHi1DdgOfcqzj7qQtZz0 Fig4A HU arrest Fig4E synchronous culture ------- COMMENT: acd1a2d2ddcf29cc 33 nXGSVrmXYOu0N7rp+7SqXAy6v/g Fig4A fin1 activation is dependent on sid1 ------- COMMENT: acd1a2d2ddcf29cc 34 XypLba/HKpZCON6K4SF7XAoKO9s Fig4A (comment: an antibody that recognized Cut12 when phosphorylated on T75) [Figure S2C] alone established that MPF phosphorylates T75 in vitro [Figure 4D]). ------- COMMENT: acd1a2d2ddcf29cc 35 wJH7L1CzLJoa71Nr8yknR2obvIU Fig4E in absence of fin1 activity dis2 remains bound to cut 12 ------- COMMENT: acd1a2d2ddcf29cc 37 BBGUFTaV0e33l+HiMg53r1f0owE cut12.1 cdc25-22 only grows at 20°C ------- COMMENT: acd1a2d2ddcf29cc 40 1uhxss5ODJkOn2vNUQ+4dTZ6O1Y Fig4F dis2 remains bound to cut12 ------- COMMENT: acd1a2d2ddcf29cc 41 mKtFJY4wIC8ueYmtUi+17gnQ2k0 Fig4F T75 T78 no longer phosphorylated and dis2 remains bound to cut12 ------- COMMENT: acd1a2d2ddcf29cc 42 3chVfETFWZ7muW/q6kcP1hzrCh4 Fig5A No increase in recruitment of plo1 to SPB when fin1 is active if T75 T78 mutated to A ------- COMMENT: acd1a2d2ddcf29cc 43 J3UhDiMk/9UbjVcbeZO65XXjMuc (comment: vw: could this one be abolished?) Fig5A No change in recruitment of plo1 to SPB when fin1 is inactivated T75 T78 mutated to D ------- COMMENT: acd1a2d2ddcf29cc 44 zDR6Lxr3A3A+xmHvCfrkHP1w5a4 Fig5A plo1 localisation to SPB is dependent on fin1 activity ------- COMMENT: acd1a2d2ddcf29cc 45 zDR6Lxr3A3A+xmHvCfrkHP1w5a4 Fig5A plo1 localisation to SPB is dependent on fin1 activity ------- COMMENT: acd1a2d2ddcf29cc 47 7dPW4m2piEI4lBTWzqMMCD/MdIA Fig5B plo1 increased specific activity ------- COMMENT: acd1a2d2ddcf29cc 48 Frk/Uz2mJ3rYvQHRoj4q7RuS5uE Fig5B. premature recruitment of protein to the mitotic SPB ------- COMMENT: acd1a2d2ddcf29cc 49 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 50 7dPW4m2piEI4lBTWzqMMCD/MdIA Fig5B plo1 increased specific activity ------- COMMENT: acd1a2d2ddcf29cc 51 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 52 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 53 7dPW4m2piEI4lBTWzqMMCD/MdIA Fig5B plo1 increased specific activity ------- COMMENT: acd1a2d2ddcf29cc 54 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 55 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 56 7dPW4m2piEI4lBTWzqMMCD/MdIA Fig5B plo1 increased specific activity ------- COMMENT: acd1a2d2ddcf29cc 57 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 58 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 59 7dPW4m2piEI4lBTWzqMMCD/MdIA Fig5B plo1 increased specific activity ------- COMMENT: acd1a2d2ddcf29cc 60 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 61 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 66 V4YP8+rMugto/vaovoPES2BSW+k (comment: CHECK promotes mitotic commitment in cut12T75AT78A) ------- COMMENT: acd1a2d2ddcf29cc 67 7dPW4m2piEI4lBTWzqMMCD/MdIA Fig5B plo1 increased specific activity ------- COMMENT: acd1a2d2ddcf29cc 68 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 69 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 70 JuFFaNvXMbhJkr2zgQDNOYnoyg8 Fig5B plo1 decreased specific activity ------- COMMENT: acd1a2d2ddcf29cc 71 vP3gYwnJ91MrfWQtYiLZ1CTGczM Fig5B (comment: CHECK DELAYED) ------- COMMENT: acd1a2d2ddcf29cc 72 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 73 JuFFaNvXMbhJkr2zgQDNOYnoyg8 Fig5B plo1 decreased specific activity ------- COMMENT: acd1a2d2ddcf29cc 74 5J1Y2YbZkaDFcUPGPHchPoNB3p8 Fig5B (comment: CHECK DELAYED) ------- COMMENT: acd1a2d2ddcf29cc 75 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 76 7dPW4m2piEI4lBTWzqMMCD/MdIA Fig5B plo1 increased specific activity ------- COMMENT: acd1a2d2ddcf29cc 77 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 78 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 79 7dPW4m2piEI4lBTWzqMMCD/MdIA Fig5B plo1 increased specific activity ------- COMMENT: acd1a2d2ddcf29cc 80 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 81 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 82 JuFFaNvXMbhJkr2zgQDNOYnoyg8 Fig5B plo1 decreased specific activity ------- COMMENT: acd1a2d2ddcf29cc 83 vP3gYwnJ91MrfWQtYiLZ1CTGczM Fig5B (comment: CHECK DELAYED) ------- COMMENT: acd1a2d2ddcf29cc 84 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 85 7dPW4m2piEI4lBTWzqMMCD/MdIA Fig5B plo1 increased specific activity ------- COMMENT: acd1a2d2ddcf29cc 86 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 87 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 88 7dPW4m2piEI4lBTWzqMMCD/MdIA Fig5B plo1 increased specific activity ------- COMMENT: acd1a2d2ddcf29cc 89 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 90 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 94 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 95 7dPW4m2piEI4lBTWzqMMCD/MdIA Fig5B plo1 increased specific activity ------- COMMENT: acd1a2d2ddcf29cc 96 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 97 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: acd1a2d2ddcf29cc 98 G0a9m9la+DaT7yfHvP9JYC89kXc Fig1H cdc25-22 cut12R531STOP can grow at higher temperature in presence of cut12. G71V mutation. (comment: vw changed form increased to normal, compared to WT) ------- COMMENT: acd1a2d2ddcf29cc 99 5fqAncwjCa7myeh6WhmWWIWN850 Fig1. Rescue of cdc25-22 but not restored to full growth (partial rescue). [ie The cut12.s11 (G71V) mutation enables cdc25+ null cells (cdc25.D) to form microcolonies of 1 to 20 cells [14]. ] ------- COMMENT: acd1a2d2ddcf29cc 100 31s9Gy/kX46wyCr/sLjEZgRbYfM Fig1. Rescue of cdc25-22 but not restored to full growth (partial rescue) ------- COMMENT: acd1a2d2ddcf29cc 101 3S7Qa/3dnJB59ftYCYEJ1bvF7zk Fig 1. Rescue of cdc25-22 but not restored to full growth (partial rescue) ------- COMMENT: acd1a2d2ddcf29cc 102 3S7Qa/3dnJB59ftYCYEJ1bvF7zk Fig 1. Rescue of cdc25-22 but not restored to full growth (partial rescue) ------- COMMENT: acd1a2d2ddcf29cc 103 31s9Gy/kX46wyCr/sLjEZgRbYfM Fig1. Rescue of cdc25-22 but not restored to full growth (partial rescue) ------- COMMENT: acd1a2d2ddcf29cc 104 31s9Gy/kX46wyCr/sLjEZgRbYfM Fig1. Rescue of cdc25-22 but not restored to full growth (partial rescue) ------- COMMENT: acd1a2d2ddcf29cc 108 M3CBRbhEfEGDD3n9lvQtTaGh2vA Fig2E ------- COMMENT: acd1a2d2ddcf29cc 109 P6Y4oAJaSIoqIIvLBCrXFfdTPuo (comment: CHECK T75T78) ------- COMMENT: acd1a2d2ddcf29cc 110 s1MM2Ng89BVn6H9UiP9URSzKTBU (comment: CHECK T75) ------- COMMENT: acd1a2d2ddcf29cc 111 vrws2ZurerKB20EHnDp8NezhGDw Fig 1b, F and G. (comment: CHECK T75T78 UNPHOSPHORYLATED FORM) ------- COMMENT: acd1a2d2ddcf29cc 112 6z5x7GPRLUVIQWPI2i/3cz6kzzQ Fig 1b, F and G. (comment: CHECK T75T78 PHOSPHORYLATED FORM) ------- COMMENT: acd1a2d2ddcf29cc 117 cJsIEQlPyulu39HafGXUh+Habdc Fig1C (comment: 2 hybrid) ------- COMMENT: acd1a2d2ddcf29cc 118 YwT2xvdza8RcwcI3oB0592I/B4g Fig1c (comment: 2-hybrid) ------- COMMENT: acd1a2d2ddcf29cc 119 cJsIEQlPyulu39HafGXUh+Habdc Fig1C (comment: 2 hybrid) ------- COMMENT: acd1a2d2ddcf29cc 120 v+a4ol+0u/wT5W5An7wRodlqDvk Fig1C (comment: 2 hybrid) ------- COMMENT: acd1a2d2ddcf29cc 121 v+a4ol+0u/wT5W5An7wRodlqDvk Fig1C (comment: 2 hybrid) ------- COMMENT: acd1a2d2ddcf29cc 122 fyAttHyLcx86WS5JOnosiswfhPg Fig2H ------- COMMENT: acd1a2d2ddcf29cc 123 IaZzwT4AfmtcFPjg6VS+eTJUuuU Fig2H (comment: VWI added this and man=de the original 'abnormal cell size' small (variable size at division, mixed sized see #3800) ------- COMMENT: acd1a2d2ddcf29cc 125 G0a9m9la+DaT7yfHvP9JYC89kXc Fig1H cdc25-22 cut12R531STOP can grow at higher temperature in presence of cut12. G71V mutation. (comment: vw changed form increased to normal, compared to WT) ------- COMMENT: acd1a2d2ddcf29cc 126 +k8l3snHnODAbS2CQHag3m5zt9Y Fig 2E phospho mimetic cdc12 mutant rescues cdc25 mutant ------- COMMENT: acd1a2d2ddcf29cc 127 +k8l3snHnODAbS2CQHag3m5zt9Y Fig 2E phospho mimetic cdc12 mutant rescues cdc25 mutant ------- COMMENT: acd1a2d2ddcf29cc 128 YwT2xvdza8RcwcI3oB0592I/B4g Fig1c (comment: 2-hybrid) ------- COMMENT: acd1a2d2ddcf29cc 129 3b3QSoBCKuA+mYiq+JcZzX6++1w Fig1D ------- COMMENT: acd89b47e1b47f21 3 j3HxK3IQKVFXWfOx3xgXARlJSIY (comment: CHECK at transcription_termination_signal) ------- COMMENT: acd89b47e1b47f21 4 j3HxK3IQKVFXWfOx3xgXARlJSIY (comment: CHECK at transcription_termination_signal) ------- COMMENT: acd89b47e1b47f21 14 j3HxK3IQKVFXWfOx3xgXARlJSIY (comment: CHECK at transcription_termination_signal) ------- COMMENT: acd89b47e1b47f21 15 j3HxK3IQKVFXWfOx3xgXARlJSIY (comment: CHECK at transcription_termination_signal) ------- COMMENT: ad37ffc7d6aa5f2a 10 wKZfkn31gcHMdS2nYmZYA8r8G7A (comment: less levere than pxl1 null) ------- COMMENT: ad37ffc7d6aa5f2a 13 wKZfkn31gcHMdS2nYmZYA8r8G7A (comment: less levere than pxl1 null) ------- COMMENT: ad37ffc7d6aa5f2a 79 d16q5ijSF/0BPlkwQFyfbSyLoMs (comment: localization dependent on filamentous actin (GO:0031941); tested using latrunculin A) ------- COMMENT: ad37ffc7d6aa5f2a 86 wxLNSr3uqqpo4TaVbQgevXfPCyE (comment: increased more than pxl1delta alone) ------- COMMENT: ad37ffc7d6aa5f2a 105 wxLNSr3uqqpo4TaVbQgevXfPCyE (comment: increased more than pxl1delta alone) ------- COMMENT: ad37ffc7d6aa5f2a 106 wxLNSr3uqqpo4TaVbQgevXfPCyE (comment: increased more than pxl1delta alone) ------- COMMENT: ad37ffc7d6aa5f2a 107 wxLNSr3uqqpo4TaVbQgevXfPCyE (comment: increased more than pxl1delta alone) ------- COMMENT: ad37ffc7d6aa5f2a 108 wxLNSr3uqqpo4TaVbQgevXfPCyE (comment: increased more than pxl1delta alone) ------- COMMENT: ad3bb22deefbbb4b 4 YoVrG5kUbw3D1QBjIDbFvSmI5m8 This mutation dissociated Mad3 from a Cdc20–C-Mad2 heterodimer when size-exclusion chromatography was performed (Supplementary Fig. 4). ------- COMMENT: ad3bb22deefbbb4b 5 YoVrG5kUbw3D1QBjIDbFvSmI5m8 This mutation dissociated Mad3 from a Cdc20–C-Mad2 heterodimer when size-exclusion chromatography was performed (Supplementary Fig. 4). ------- COMMENT: ad3bb22deefbbb4b 8 B7Gnqq2ZKgDM4Ndm81TB404qWnw Disrupting the Leu-binding pocket by repla- cing Val 196 with a bulky methionine, and substituting Ser for the Cdh1 equivalents of Asp 173, Asp 457 and Glu 458 at the putative Arg- binding site, eliminated the ability of Cdh1 to stimulate APC/C activity (Fig. 4e and Supplementary Fig. 6) but had no affect on co-activator binding to the APC/C (Supplementary Fig. 7) ------- COMMENT: ad3cea5e630841a3 3 qlFuljPBXBG9g4BNyhNXVwSitWI (comment: CHECK regulator of structure-specific DNA nuclease) ------- COMMENT: ad3cea5e630841a3 17 NBDV9KBV9kRS0BTWzTHFUkxM6nQ (comment: not sure) ------- COMMENT: ad5cdccac663e13f 11 JBQwJgbRBR+dlg5jsdWChWv95aE (comment: tyrosine; residue not determined) ------- COMMENT: ad5cdccac663e13f 13 JBQwJgbRBR+dlg5jsdWChWv95aE (comment: tyrosine; residue not determined) ------- COMMENT: adc0f6d0a5166829 1 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: adc0f6d0a5166829 2 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: adc0f6d0a5166829 3 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: adc0f6d0a5166829 4 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: adc0f6d0a5166829 5 lOG9gw8xks4VnxcVpIEbKoXCUak Fig. 1C, D and 2A, E ------- COMMENT: adc0f6d0a5166829 6 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: adc0f6d0a5166829 7 sbrJ/5Vo1V8pKyRS+c0pdprXxDc Plo1 localization to the contractile ring was abolished in the Mid1-T517A mutant (Figures S1C and S1D; 0 of 66 mitotic T517A cells with Plo1-GFP at the contractile ring compared to 26 of 58 in control mitotic cells) ------- COMMENT: adc0f6d0a5166829 8 Q9GQSc0zICU2zI+p1mmG6YUBoww Fig. 2A and E ------- COMMENT: adc0f6d0a5166829 9 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: adc0f6d0a5166829 10 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: adc0f6d0a5166829 11 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: adc0f6d0a5166829 12 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: adc0f6d0a5166829 13 +Ek/y8FI1jr63JOLTj8F9c2kaEQ Fig. S2B ------- COMMENT: adc0f6d0a5166829 14 +Ek/y8FI1jr63JOLTj8F9c2kaEQ Fig. S2B ------- COMMENT: adc0f6d0a5166829 15 +Ek/y8FI1jr63JOLTj8F9c2kaEQ Fig. S2B ------- COMMENT: adc0f6d0a5166829 16 +Ek/y8FI1jr63JOLTj8F9c2kaEQ Fig. S2B ------- COMMENT: adc0f6d0a5166829 17 +Ek/y8FI1jr63JOLTj8F9c2kaEQ Fig. S2B ------- COMMENT: adc0f6d0a5166829 18 +Ek/y8FI1jr63JOLTj8F9c2kaEQ Fig. S2B ------- COMMENT: adc0f6d0a5166829 19 +Ek/y8FI1jr63JOLTj8F9c2kaEQ Fig. S2B ------- COMMENT: adc0f6d0a5166829 20 +Ek/y8FI1jr63JOLTj8F9c2kaEQ Fig. S2B ------- COMMENT: adc0f6d0a5166829 21 EJrKWyBr/NaQhxxvZLUQXKV3oNU Fig. S2E ------- COMMENT: adc0f6d0a5166829 22 EJrKWyBr/NaQhxxvZLUQXKV3oNU Fig. S2E ------- COMMENT: adc0f6d0a5166829 23 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: adc0f6d0a5166829 24 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: adc0f6d0a5166829 25 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: adc0f6d0a5166829 26 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: adc0f6d0a5166829 27 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: adc0f6d0a5166829 28 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: adc0f6d0a5166829 29 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: adc0f6d0a5166829 30 9d4Ox2sg4MZM5ohOY5Y3J7qZ91Y Fig. S3H ------- COMMENT: adc0f6d0a5166829 31 9d4Ox2sg4MZM5ohOY5Y3J7qZ91Y Fig. S3H ------- COMMENT: adc0f6d0a5166829 32 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: adc0f6d0a5166829 33 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: adc0f6d0a5166829 34 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: adc0f6d0a5166829 35 7uDs73lOpIsv8+BNgHXuk9kGn4Q These data altogether reveal mechanisms by which Plo1 acts as a key temporal coordinator of contractile ring assembly events in fission yeast. ------- COMMENT: adc0f6d0a5166829 36 /5bIE1BYljb1ex5uYPnPFZ/VEvI Plo1 phosphorylates several residues within the first 100 amino acids of Mid1, which directly interact with the IQGAP Rng2 [17], and influences the timing of myosin II recruitment. Plo1 thereby facilitates contractile ring assembly at mitotic onset. ------- COMMENT: add2e9acc505616a 1 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: add2e9acc505616a 2 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: add2e9acc505616a 3 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: add2e9acc505616a 4 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: add2e9acc505616a 5 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: add2e9acc505616a 6 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: add2e9acc505616a 7 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: add2e9acc505616a 8 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: add2e9acc505616a 9 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: add2e9acc505616a 10 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: add2e9acc505616a 11 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: add2e9acc505616a 12 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: adf22222853d160f 12 /JN8MOaJPdWg26zAjQv2nFiAock (comment: same severity as wee1-50 alone) ------- COMMENT: adf4805a233dc936 1 jg+8xt1rmfYMmrZFbvGztSVrayk (comment: three-hybrid assay; also binds exogenous ESEs) ------- COMMENT: ae0a54cf0c62977c 1 kBRRthcuuYvBI36bFJNCthswXkU Quantification of the results shown in Fig. 4A is illustrated in Fig. 4B. Taken together, these experiments reveal that PC4 stimulates the rate of preinitiation complex formation. ------- COMMENT: ae1c2b254e8c3cee 1 B+NbSWP4sKR8MSqN60qnKVLxsHM ChIP assay indicated that Rad21 associates with ribosomal DNA (rDNA) rather than telomere-associated sequences (see below). Moreover, fluorescence microscopy revealed that Rad21 tagged with GFP colocalizes mostly with a nucleolar protein, Gar1 (3), tagged with CFP in the horsetail nucleus (Fig. 1A). These results suggest that Rad21 is enriched in the nucleolus, rather than telomere-adjacent DNA sequences, although residual association with other chromosomal regions might also occur ------- COMMENT: ae1c2b254e8c3cee 2 tTP9VcxBSMlA8Mz1u9GvegXNmkI . The rad21-K1 mutation by itself showed no meiotic defect. However, when rec8 was combined with rad21-K1, the equational segregation at meiosis I was partly disrupted with the reductional population increasing to 40% (Fig. 2B). ------- COMMENT: ae1c2b254e8c3cee 3 11PMQ3nlLc4GuCBqX9+OfzYIQls We conclude that Rad21 plays a role in ensuring equational segregation during meiosis I in rec8 cells. ------- COMMENT: ae3cdbcd00418954 1 2Z3jOz3IEJZ4WnNRFH7upIpUlos full length of Pkd2 (GFP-Pkd2) colocalized with Pmr1, a marker for the ER (Nakazawa et al., 2019), ------- COMMENT: ae3cdbcd00418954 3 cjfoXhwJYR3Q9P2zepbsXXXLMPs In accordance with previous observation, the colony did not form under the inducible condition of pkd2+ overexpres- sion (Figure 1b). ------- COMMENT: ae3cdbcd00418954 4 oQkBkTIJnqAbFp1jeemGNh4pN+8 Although the CDRE signal is disap- peared (Figure S1B), the cells did not grow in inducible condition in prz1 deletion background (Figure 1b), suggesting that the activation of calcium signaling and the cytotoxicity induced by overexpression of pkd2+ are independent. ------- COMMENT: ae3cdbcd00418954 5 uDmI4Nv85lcbYvEhlpeUlWqA13w Interestingly, the internal transmem- brane region was sufficient to induce both CDRE activa- tion and growth inhibition (Figure 1a,d). ------- COMMENT: ae3cdbcd00418954 6 +6wX9lzEjkIHLybFa+DTyi7A+PM Overexpression of either construct inhibited the growth like full length (Figure 1d), indicating that each of them is capa- ble of inducing cytotoxicity upon overexpression. ------- COMMENT: ae3cdbcd00418954 7 +6wX9lzEjkIHLybFa+DTyi7A+PM Overexpression of either construct inhibited the growth like full length (Figure 1d), indicating that each of them is capa- ble of inducing cytotoxicity upon overexpression. ------- COMMENT: ae3cdbcd00418954 9 lx8jINhjagTtIZb+reZ03uLoE4g Localizations of Pkd2ΔN170, Pkd2ΔC577, or Pkd2TM were identical to full length Pkd2 (Figures 2b and S2A). ------- COMMENT: ae3cdbcd00418954 10 lx8jINhjagTtIZb+reZ03uLoE4g Localizations of Pkd2ΔN170, Pkd2ΔC577, or Pkd2TM were identical to full length Pkd2 (Figures 2b and S2A). ------- COMMENT: ae3cdbcd00418954 11 lx8jINhjagTtIZb+reZ03uLoE4g Localizations of Pkd2ΔN170, Pkd2ΔC577, or Pkd2TM were identical to full length Pkd2 (Figures 2b and S2A). ------- COMMENT: ae3cdbcd00418954 12 O3NCVLruW72Rew79DvYkGGZGx38 N-terminus of Pkd2 (Pkd2N) localized to the cytoplasm and slightly to the ER, ------- COMMENT: ae3cdbcd00418954 13 NMP1PuKpjIlabQJ/5ThNxrqsk0Y whereas C-terminus of Pkd2 (Pkd2C) displayed a uniform cytoplasmic pattern (Figures 2b and S2A). ------- COMMENT: ae3cdbcd00418954 14 qksCVULv7eJkdoPZgxdQeE81zIo localized to the septum and the plasma membrane, especially enriched at cell tips (Figure 2d). ------- COMMENT: ae3cdbcd00418954 15 qksCVULv7eJkdoPZgxdQeE81zIo localized to the septum and the plasma membrane, especially enriched at cell tips (Figure 2d). ------- COMMENT: ae3cdbcd00418954 16 lrN5Ii5sV3GQJJ8Igb5lvn1D8HY Figure 3b, (comment: tetrad analysis) ------- COMMENT: ae3cdbcd00418954 17 sUauz89MRbilTyzOgqtuUo8y0Bg Figure 3b,(comment: tetrad analysis) ------- COMMENT: ae3cdbcd00418954 18 ir2Ucwuo1jQhmED4MiVHDAxD7Og Although C-terminal deleted cells (mCh-pkd2ΔC) did not affect to the growth under the normal condition, the strain was hypersensitive to CaCl2 (Figure 3c) ------- COMMENT: ae3cdbcd00418954 19 ir2Ucwuo1jQhmED4MiVHDAxD7Og Although C-terminal deleted cells (mCh-pkd2ΔC) did not affect to the growth under the normal condition, the strain was hypersensitive to CaCl2 (Figure 3c) ------- COMMENT: ae47abf8322de31d 1 Krj255esPTVnNiE5+IjBa6k2AuY Figure. 5A ------- COMMENT: ae47abf8322de31d 2 c+hzXJEbcUcGwNy4EqlFLAmRW7Q Figure. 6A ------- COMMENT: ae593bb3040d3c61 1 JMqCVvKTsr7EJ5VMJnVI0pfR/FM Table 1. List of the 13 TAM-sensitive heterozygous strains/ Fig. 2. Confirmation of the tamoxifen (TAM)-sensitive candidate strains by spotting assays ------- COMMENT: ae593bb3040d3c61 2 nEggoERJj3v8Xrgs67uCpLWN4zk Table 1. List of the 13 TAM-sensitive heterozygous strains/Fig. 2. Confirmation of the tamoxifen (TAM)-sensitive candidate strains by spotting assays ------- COMMENT: ae593bb3040d3c61 3 nEggoERJj3v8Xrgs67uCpLWN4zk Table 1. List of the 13 TAM-sensitive heterozygous strains/Fig. 2. Confirmation of the tamoxifen (TAM)-sensitive candidate strains by spotting assays ------- COMMENT: ae593bb3040d3c61 4 nEggoERJj3v8Xrgs67uCpLWN4zk Table 1. List of the 13 TAM-sensitive heterozygous strains/Fig. 2. Confirmation of the tamoxifen (TAM)-sensitive candidate strains by spotting assays ------- COMMENT: ae593bb3040d3c61 5 nEggoERJj3v8Xrgs67uCpLWN4zk Table 1. List of the 13 TAM-sensitive heterozygous strains/Fig. 2. Confirmation of the tamoxifen (TAM)-sensitive candidate strains by spotting assays ------- COMMENT: ae593bb3040d3c61 6 nEggoERJj3v8Xrgs67uCpLWN4zk Table 1. List of the 13 TAM-sensitive heterozygous strains/Fig. 2. Confirmation of the tamoxifen (TAM)-sensitive candidate strains by spotting assays ------- COMMENT: ae593bb3040d3c61 7 nEggoERJj3v8Xrgs67uCpLWN4zk Table 1. List of the 13 TAM-sensitive heterozygous strains/Fig. 2. Confirmation of the tamoxifen (TAM)-sensitive candidate strains by spotting assays ------- COMMENT: ae593bb3040d3c61 8 nEggoERJj3v8Xrgs67uCpLWN4zk Table 1. List of the 13 TAM-sensitive heterozygous strains/Fig. 2. Confirmation of the tamoxifen (TAM)-sensitive candidate strains by spotting assays ------- COMMENT: ae593bb3040d3c61 9 nEggoERJj3v8Xrgs67uCpLWN4zk Table 1. List of the 13 TAM-sensitive heterozygous strains/Fig. 2. Confirmation of the tamoxifen (TAM)-sensitive candidate strains by spotting assays ------- COMMENT: ae593bb3040d3c61 10 8brgMkN9GKnXkI2l8LhAsUTAZms Table 1. List of the 13 TAM-sensitive heterozygous strainsFig. 2. Confirmation of the tamoxifen (TAM)-sensitive candidate strains by spotting assays ------- COMMENT: ae593bb3040d3c61 11 JMqCVvKTsr7EJ5VMJnVI0pfR/FM Table 1. List of the 13 TAM-sensitive heterozygous strains/ Fig. 2. Confirmation of the tamoxifen (TAM)-sensitive candidate strains by spotting assays ------- COMMENT: ae593bb3040d3c61 12 nEggoERJj3v8Xrgs67uCpLWN4zk Table 1. List of the 13 TAM-sensitive heterozygous strains/Fig. 2. Confirmation of the tamoxifen (TAM)-sensitive candidate strains by spotting assays ------- COMMENT: ae593bb3040d3c61 13 vVWubXS+S5gXaCTbI1mX+u+wKeM Even without TAM treatment, the vps54 heterozygous mutant showed a high penetrance of enlarged vesicles (red arrows in Fig. 3) without any detectable change in cell shape (Fig. 3) and growth fitness (Fig. 2), compared with the SP286 control strain (comment: vw: cells are in fact quite misshapen) ------- COMMENT: ae593bb3040d3c61 14 f1HUikB+dZU1UfCRd5O5VK33xsY When treated with TAM, the vps54 heterozygous mutants showed more enlarged vesicles (yellow ar- rows in Fig. 3) ....., com- pared with the SP286 control. ------- COMMENT: ae5f72244ad85ad1 1 /0qiqEQJlugWDmwHHOsCAICXE0U rescues SPB separation defects of cut7∆ pkl1∆ in meiosis I ------- COMMENT: ae5f72244ad85ad1 2 /0qiqEQJlugWDmwHHOsCAICXE0U rescues SPB separation defects of cut7∆ pkl1∆ in meiosis I ------- COMMENT: ae5f72244ad85ad1 3 Zutm5rpXvm4EThFuP+i9MYEj7jQ temperature sensitivity of cut7∆ pkl1∆ cells deteriorated by rad21-K1 ------- COMMENT: ae5f72244ad85ad1 4 NOSZBHTaw2Nms38rVQiGIBhVlh0 temperature sensitivity of cut7∆ pkl1∆ cells deteriorated by swi6∆ ------- COMMENT: ae5f72244ad85ad1 5 8DqfjIFLEmqACSOjDGQWUdpnu6k The Nuf2-containing kinetochore complex serves as a physical fulcrum for microtubule-dependent SPB separation ------- COMMENT: ae5f72244ad85ad1 6 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: ae5f72244ad85ad1 7 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: ae5f72244ad85ad1 8 ORN6xvOEvljQrut/cXvDxHZu2Ew (comment: single nucleus) ------- COMMENT: ae5f72244ad85ad1 11 zoHfVWiJFnpiVdfesQPHiAk45w4 Figure 1D,(comment: E . monopolar?) ------- COMMENT: ae5f72244ad85ad1 12 Mq9+krVBzhxJ9XJN+XX3eBacTRc figure 1B, D ------- COMMENT: ae5f72244ad85ad1 13 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: ae5f72244ad85ad1 14 ecY54SbAZnrTnHLHpKxXXPVE+sE (comment: I didn't check the supp, but probably can only make this annotation?) ------- COMMENT: ae5f72244ad85ad1 15 0d6Iljs+jkrHHWv0Mkxpth00ZuY top, Figure 3B, C) ------- COMMENT: ae5f72244ad85ad1 16 XMpC5eXESTc98e7GuFW0L75xCAQ (comment: meiosis I inital ) Figure 3B, C ------- COMMENT: ae5f72244ad85ad1 17 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: ae5f72244ad85ad1 18 VsMm3Kx6TDhI0FadLzY87SmSG1I Figure S3B, C (comment: pentrance, frequently like quadruple) ------- COMMENT: ae5f72244ad85ad1 19 guV+jWdX7ogRDSIllFllYA2Dk9c 28 ~ 32 min, Figure 3B; and 24 ~ 28 min, Figure S3B) ------- COMMENT: ae5f72244ad85ad1 20 guV+jWdX7ogRDSIllFllYA2Dk9c 28 ~ 32 min, Figure 3B; and 24 ~ 28 min, Figure S3B) ------- COMMENT: ae5f72244ad85ad1 21 sRbiU/jtYRJxVkHoyWOhqsXRQaU Figure S5 ------- COMMENT: ae5f72244ad85ad1 22 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: ae5f72244ad85ad1 23 his1Usb45H+JWI97J7s/8XaZptQ Figure 5C, D ------- COMMENT: ae5f72244ad85ad1 24 0JtWWbGerTmkzK8lAu6rlAYe7tU Figure 5E ------- COMMENT: ae5f72244ad85ad1 25 0JtWWbGerTmkzK8lAu6rlAYe7tU Figure 5E ------- COMMENT: ae5f72244ad85ad1 26 xHgWiQGxcgBO0xJRn3WgCICGeX4 Figure S6 ------- COMMENT: ae5f72244ad85ad1 27 Q98TjDJEsp0iAoJ2tca71l2ZnUQ Figure 5G (comment: this term referes to initial) ------- COMMENT: ae5f72244ad85ad1 28 EIIPiRrvfcS+FfgdZEmIAytBkSg Figure 5H ------- COMMENT: ae5f72244ad85ad1 29 OMKzAmQE5yfXBBhH6HFjMlTL3aY Figure 5I ------- COMMENT: ae5f72244ad85ad1 30 bQrAvw5v3pdeGEjTasNZSNQl2dw Figure 6A ------- COMMENT: ae5f72244ad85ad1 31 9OvAQV+BuTaKNlgi3CemQA9dtZE Figure 6D, E ------- COMMENT: ae5f72244ad85ad1 32 FT8WC/x5fJzuB8iEqPOe2hiI67U meiotic . The Nuf2-containing kinetochore complex serves as a physical fulcrum for microtubule-dependent SPB separation ------- COMMENT: aeaa06c0eac76f9e 1 UTCk7A3+Ze39Dv3TTBQi62R7J28 figure 1A, B ------- COMMENT: aeaa06c0eac76f9e 13 8Fsj/yPjVUD6HK6J9QggLr4qNJM non detectable Fig. 1. Phosphate starvation induces ecl3+ expression in a pho7+-dependent manner. ------- COMMENT: aeaa06c0eac76f9e 16 6hnmNExo63/ENmLPBFnYalxuADs phosphate replete. Zfs1 is involved in the repression of the ecl3+ transcript level in a nutrient-rich environment but is not required for the induction by phosphate starvation. Next, ------- COMMENT: aeaa06c0eac76f9e 17 0/z3OTyn3CUg6iXekmlbOAkI9n0 Phosphate starvation did not induce ecl3+ expression in Δckb1 cells, indicating that the induction was dependent on Ckb1 (Fig. 2B) ------- COMMENT: aeadfc528237dc96 3 ls8VkmkVrF1IaHDRMVqJNPZiNBI (comment: they show transfer to a heterologous cytochrome p450 enzyme, but pombe doesn't have any mitochondrial ones.) ------- COMMENT: aeb3d0063f6b810 1 GiiMrtrOnSIoTDNyiRzkA3FJuqA (comment: PHEROMONE) ------- COMMENT: aebb22b799de8e58 1 7eX/ah1f8sR/7viFtvdYlNxjEyI As previously reported, deletion of rrp6 leads to strong accumulation of CUTs and a small group of mRNAs that are mostly involved in meiosis16,23,25,29,30,35. ------- COMMENT: aebb22b799de8e58 2 7eX/ah1f8sR/7viFtvdYlNxjEyI As previously reported, deletion of rrp6 leads to strong accumulation of CUTs and a small group of mRNAs that are mostly involved in meiosis16,23,25,29,30,35. ------- COMMENT: aebb22b799de8e58 4 9sFRunUmCT5+vkaCNpda9Nl/erU Surprisingly, deletion of the S. pombe Trf4/5 orthologue, cid14, showed only a minor effect on CUTs at a genome-wide level (Fig. 2a,b) ------- COMMENT: aebb22b799de8e58 5 XuBBX5gEVjMOzDBQ0XYRG09yh14 In contrast, deletion or mutation alleles of the MTREC complex lead to significant accumulation of all types of CUTs and also meiotic mRNAs. This effect is comparable to the level of CUT accumulation in the nuclear exosome subunit rrp6 deletion (Fig. 2a,b) ------- COMMENT: aebb22b799de8e58 6 XuBBX5gEVjMOzDBQ0XYRG09yh14 In contrast, deletion or mutation alleles of the MTREC complex lead to significant accumulation of all types of CUTs and also meiotic mRNAs. This effect is comparable to the level of CUT accumulation in the nuclear exosome subunit rrp6 deletion (Fig. 2a,b) ------- COMMENT: aebb22b799de8e58 7 XuBBX5gEVjMOzDBQ0XYRG09yh14 In contrast, deletion or mutation alleles of the MTREC complex lead to significant accumulation of all types of CUTs and also meiotic mRNAs. This effect is comparable to the level of CUT accumulation in the nuclear exosome subunit rrp6 deletion (Fig. 2a,b) ------- COMMENT: aebb22b799de8e58 8 XuBBX5gEVjMOzDBQ0XYRG09yh14 In contrast, deletion or mutation alleles of the MTREC complex lead to significant accumulation of all types of CUTs and also meiotic mRNAs. This effect is comparable to the level of CUT accumulation in the nuclear exosome subunit rrp6 deletion (Fig. 2a,b) ------- COMMENT: aebb22b799de8e58 9 XuBBX5gEVjMOzDBQ0XYRG09yh14 In contrast, deletion or mutation alleles of the MTREC complex lead to significant accumulation of all types of CUTs and also meiotic mRNAs. This effect is comparable to the level of CUT accumulation in the nuclear exosome subunit rrp6 deletion (Fig. 2a,b) ------- COMMENT: aebb22b799de8e58 10 XuBBX5gEVjMOzDBQ0XYRG09yh14 In contrast, deletion or mutation alleles of the MTREC complex lead to significant accumulation of all types of CUTs and also meiotic mRNAs. This effect is comparable to the level of CUT accumulation in the nuclear exosome subunit rrp6 deletion (Fig. 2a,b) ------- COMMENT: aebb22b799de8e58 11 vfNVU029nWi1b6oIIchIP1wbTyY Deletion of iss10 or mmi1 only affects meiotic mRNAs ------- COMMENT: aebb22b799de8e58 12 vfNVU029nWi1b6oIIchIP1wbTyY Deletion of iss10 or mmi1 only affects meiotic mRNAs ------- COMMENT: aebb22b799de8e58 13 QG+WDoSsVhfsf3JWEw48aADIhPM meiotic genes ....Overall, our experiments show that the MTREC complex is specifically recruited to CUTs and meiotic mRNAs, and it plays a key role in their degradation by the nuclear exosome. ------- COMMENT: aebb22b799de8e58 14 ox8dHj4Om5IsQW3PBbI1+51Z8Ic (comment: exosome dependent) ------- COMMENT: aebb22b799de8e58 15 Dn0OvOocipGIvMACylRWlqj74FI Similar to previous reports, we detected significantly increased intronic reads in the exosome mutant rrp6D strain (Fig. 4a,b). ------- COMMENT: aebb22b799de8e58 16 Dn0OvOocipGIvMACylRWlqj74FI Similar to previous reports, we detected significantly increased intronic reads in the exosome mutant rrp6D strain (Fig. 4a,b). ------- COMMENT: aebb22b799de8e58 17 Dn0OvOocipGIvMACylRWlqj74FI Similar to previous reports, we detected significantly increased intronic reads in the exosome mutant rrp6D strain (Fig. 4a,b). ------- COMMENT: aebb22b799de8e58 18 Dn0OvOocipGIvMACylRWlqj74FI Similar to previous reports, we detected significantly increased intronic reads in the exosome mutant rrp6D strain (Fig. 4a,b). ------- COMMENT: aebb22b799de8e58 19 hfe2S+YI6U8nfrzBeCJTYWyVXkc This analysis revealed that in the ctr1D or nrl1D mutant strains, both the intronic and also the surrounding exonic sequence coverage showed similar increases, while the expression of genes without introns was unaffected (Supplementary Fig. 4c,d). This result strongly suggests that the elevated level of intronic reads in the mutant strains is the consequence of the inefficient degradation of unspliced or mis- ------- COMMENT: aebb22b799de8e58 20 ox8dHj4Om5IsQW3PBbI1+51Z8Ic (comment: exosome dependent) ------- COMMENT: aebb22b799de8e58 21 Dn0OvOocipGIvMACylRWlqj74FI Similar to previous reports, we detected significantly increased intronic reads in the exosome mutant rrp6D strain (Fig. 4a,b). ------- COMMENT: aebb22b799de8e58 22 Dn0OvOocipGIvMACylRWlqj74FI Similar to previous reports, we detected significantly increased intronic reads in the exosome mutant rrp6D strain (Fig. 4a,b). ------- COMMENT: aebb22b799de8e58 23 ox8dHj4Om5IsQW3PBbI1+51Z8Ic (comment: exosome dependent) ------- COMMENT: aebb22b799de8e58 24 ox8dHj4Om5IsQW3PBbI1+51Z8Ic (comment: exosome dependent) ------- COMMENT: aed076cba91d48c9 1 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: aed076cba91d48c9 2 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: aed076cba91d48c9 3 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: aed076cba91d48c9 4 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: aed076cba91d48c9 5 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: aed076cba91d48c9 6 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: aed076cba91d48c9 7 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: aed076cba91d48c9 8 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: aed076cba91d48c9 9 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: aed076cba91d48c9 10 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: aed076cba91d48c9 11 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: aed076cba91d48c9 12 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: aed076cba91d48c9 13 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: aed076cba91d48c9 14 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: aed076cba91d48c9 15 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: aed076cba91d48c9 16 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: aed076cba91d48c9 17 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: aed076cba91d48c9 18 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: aed076cba91d48c9 19 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: aed076cba91d48c9 20 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: aed076cba91d48c9 21 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: aed076cba91d48c9 22 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: aed076cba91d48c9 23 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: aed076cba91d48c9 24 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: aed076cba91d48c9 25 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: aed076cba91d48c9 26 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: aed076cba91d48c9 27 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: aed076cba91d48c9 28 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: aed076cba91d48c9 29 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: aed076cba91d48c9 30 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: aed076cba91d48c9 31 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: aed076cba91d48c9 32 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: aed076cba91d48c9 33 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: aed076cba91d48c9 34 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: aed076cba91d48c9 35 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: aed076cba91d48c9 36 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: aed076cba91d48c9 37 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: aed076cba91d48c9 38 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: aed076cba91d48c9 39 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: aed076cba91d48c9 40 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: aed076cba91d48c9 41 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: aed076cba91d48c9 42 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: aed076cba91d48c9 43 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: aed076cba91d48c9 44 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: aed076cba91d48c9 45 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: aed076cba91d48c9 46 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: aed076cba91d48c9 47 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: aed076cba91d48c9 48 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: aed076cba91d48c9 49 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: aed076cba91d48c9 50 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: aed076cba91d48c9 51 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: aed076cba91d48c9 52 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: aed076cba91d48c9 53 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: aed076cba91d48c9 54 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: aed076cba91d48c9 55 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: aed076cba91d48c9 56 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: aed076cba91d48c9 57 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: aed076cba91d48c9 58 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: aed076cba91d48c9 59 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: aed076cba91d48c9 60 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: aed076cba91d48c9 61 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: aed076cba91d48c9 62 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: aed076cba91d48c9 63 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: aed076cba91d48c9 64 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: aed076cba91d48c9 65 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: aed076cba91d48c9 66 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: aed076cba91d48c9 67 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: aed076cba91d48c9 68 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: aed076cba91d48c9 75 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: aed076cba91d48c9 76 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: aedeeafb353d00c4 1 heyE7ur7W8A/8AhEyP02Ag4BPxE Fig.1d, e, f ------- COMMENT: aedeeafb353d00c4 2 6AY9yt8TA6UngrDKjYYejowSzzM Fig1d ------- COMMENT: aedeeafb353d00c4 3 6AY9yt8TA6UngrDKjYYejowSzzM Fig1d ------- COMMENT: aedeeafb353d00c4 4 6AY9yt8TA6UngrDKjYYejowSzzM Fig1d ------- COMMENT: aedeeafb353d00c4 5 6AY9yt8TA6UngrDKjYYejowSzzM Fig1d ------- COMMENT: aedeeafb353d00c4 6 kaslpacDJVemQlm5xit1fDPTnkg Fig1f (comment: evidence:immunoblot using anti-thymine dimer anitbodies) ------- COMMENT: aedeeafb353d00c4 7 I1gD4nTxgzPR9qMpR5VT+UcBbQI Fig1g ------- COMMENT: aedeeafb353d00c4 8 Mf+MS2WyaHPjhbcTU/baUDc1grc Fig1h ------- COMMENT: aedeeafb353d00c4 9 6mkVV5reODyrII6qdYSh1yazri8 Fig2e ------- COMMENT: aedeeafb353d00c4 10 6mkVV5reODyrII6qdYSh1yazri8 Fig2e ------- COMMENT: aedeeafb353d00c4 32 Kdsrpl6ZPqo/QoolfS9ZRlUDrZ4 Fig3a-g (comment: evidence: immunpflouresence) ------- COMMENT: aedeeafb353d00c4 33 h09KQpN7u/q6m3yDchMr2e10lc8 Fig4 ------- COMMENT: aedeeafb353d00c4 34 U1NiAwkwFveDm8425RQEx2O6HgY Fig5 ------- COMMENT: aedeeafb353d00c4 36 U1NiAwkwFveDm8425RQEx2O6HgY Fig5 ------- COMMENT: aedeeafb353d00c4 39 1HG7HkAze7m1hZX5NVXtHu9jutE Fig7 ------- COMMENT: aedeeafb353d00c4 41 WFFG7BeQrcemZIOAhVie47z+qJw Temperature sensitivity of cut14-Y1 (L543S) is suppressed by cut14 A527V, N533K, Q540R, S543L, S543T, G549D, G594D, G612S, or T641V. ------- COMMENT: aedeeafb353d00c4 45 WzpDwR/lAN26oOWU47HpZCoKeLw fig 2b, c ------- COMMENT: aedeeafb353d00c4 46 WzpDwR/lAN26oOWU47HpZCoKeLw fig 2b, c ------- COMMENT: aedeeafb353d00c4 57 rOKtcjWuBKJkZhBlKipKFHfPyNg Data from three experiments: DNA reannealing (renaturation assay) using heat-denatured DNA (ssDNA); Removal of RPA proteins associated with ssDNA, RPA-coated heat-denatured DNA (ssDNA) is renaturated; Removal of RNA associated with ssDNA, RNA/DNA hybrid is denatured by renaturation activity of condensin SMC ------- COMMENT: aee38813cc53380f 1 FYgoTKgpiru7KKe01yWLc5ueVAY (comment: dominat negative) ------- COMMENT: aee38813cc53380f 3 GcBEIy9kZckr+Ha5rjSkPzPEUtc Rad24-E185K mutant reduces interaction with Byr2 ------- COMMENT: aee38813cc53380f 4 ECs1jXXr13rxCCyuWQuzao+hojM Rad24-E185K mutant reduces interaction with Mei2 ------- COMMENT: aee38813cc53380f 5 I4U8qj1tvA4CNtJ+esIKLxzPpQc Rad24-E185K mutant reduces interaction with Ste11 ------- COMMENT: aee38813cc53380f 6 OdnZUAj3Aw1JDmuqaxyjyH/W1ow Rad24-E185K mutant retains interaction with Chk1 ------- COMMENT: aee38813cc53380f 13 dE9El1bJJPPANQwQsCHgfnbrGrs (comment: CHECK in sam3 mutant) ------- COMMENT: aee38813cc53380f 18 ojBD37o60OgtAxTbAs9VD8ftl8o (comment: CHECK by expression of rad24-E185K) ------- COMMENT: aee38813cc53380f 19 DvZdhpjSEUWPXCtIKPd4beAihUA exclusion from nucleoplasm is delayed in rad24 deletion background ------- COMMENT: aee38813cc53380f 21 dE9El1bJJPPANQwQsCHgfnbrGrs (comment: CHECK in sam3 mutant) ------- COMMENT: aee38813cc53380f 25 TDBs4Gwlvf7ob1mE0s5frWe2nU4 Rad24-E185K mutant retains interaction with Rad25 ------- COMMENT: af315062024a2cc5 8 YBsaO0Gzy5Iavf8E+ye0FUTjroc Fig 4a ------- COMMENT: af3180f6af4fa627 1 XD7s4lcWkZaGO+Tw6teR9tYt760 speckles in Fig. 4A ------- COMMENT: af39cc71da933c2d 1 pU6QwxYE4uBPkDsU5ixwpLiKe9w asp1∆ based pho1 gene hyper-repression suppressed by rad24-5'UTR- ACG -> ATG ------- COMMENT: af39cc71da933c2d 2 8RlPMPLYXrVdyDK7aPSL8OHRv2Q asp1∆ based pho1 gene hyper-repression suppressed by csk1-Asn284Lys, Arg285Trp, Ala286Gly ------- COMMENT: af39cc71da933c2d 3 YC8fS/BMw1xMx8hlT3mu1Ilj1AU asp1∆ based pho1 gene hyper-repression suppressed by SPBP8B7.17c Gly271Asp ------- COMMENT: af39cc71da933c2d 4 njXM//vh1hefDEpKg8cGrdDpMbw asp1∆ based pho1 gene hyper-repression suppressed by tnr3-Cys545Tyr ------- COMMENT: af39cc71da933c2d 5 U5xVd29zXar9cgFR7Q1DkERhNEc asp1∆ based pho1 gene hyper-repression suppressed by tnr3-Asp460Asn ------- COMMENT: af39cc71da933c2d 25 qwCPJbNQr4oLTXZJOC4djufCn78 (comment: CHECK Decreased acid phosphatase activity) ------- COMMENT: af39cc71da933c2d 26 qwCPJbNQr4oLTXZJOC4djufCn78 (comment: CHECK Decreased acid phosphatase activity) ------- COMMENT: af39cc71da933c2d 27 qwCPJbNQr4oLTXZJOC4djufCn78 (comment: CHECK Decreased acid phosphatase activity) ------- COMMENT: af39cc71da933c2d 28 qwCPJbNQr4oLTXZJOC4djufCn78 (comment: CHECK Decreased acid phosphatase activity) ------- COMMENT: af39cc71da933c2d 29 qwCPJbNQr4oLTXZJOC4djufCn78 (comment: CHECK Decreased acid phosphatase activity) ------- COMMENT: af39cc71da933c2d 40 ZjSqeLrKRNFMzmuQisDd0m3JQ4U (comment: Northern Blotting, RNA-Seq) ------- COMMENT: af39cc71da933c2d 41 NWi6MUAC7IaiU0kU7SuYme/+WIo (comment: RNA-Seq) ------- COMMENT: af39cc71da933c2d 42 Veu1EZG8jWNRdK1Vvy/tDD588A8 (comment: CHECK Increased acid phosphatase activity) ------- COMMENT: af542192349c135a 1 uh5g9oeqXc/OWAihnDGWAssGpB0 Figure 1H ------- COMMENT: af542192349c135a 3 aOqYL7F2KV5d9E4mVC2uoiGmluk Figure 1E ------- COMMENT: af542192349c135a 4 aOqYL7F2KV5d9E4mVC2uoiGmluk Figure 1E ------- COMMENT: af542192349c135a 5 aOqYL7F2KV5d9E4mVC2uoiGmluk Figure 1E ------- COMMENT: af542192349c135a 6 tllLUt9cplMmj2K53Ho9PnyjXm0 Figure 1G ------- COMMENT: af542192349c135a 7 iAcbpl1ew+nFwb8AiAqOLZxpvN8 Figure 1D ------- COMMENT: af542192349c135a 9 uh5g9oeqXc/OWAihnDGWAssGpB0 Figure 1H ------- COMMENT: af542192349c135a 10 uh5g9oeqXc/OWAihnDGWAssGpB0 Figure 1H ------- COMMENT: af542192349c135a 11 uh5g9oeqXc/OWAihnDGWAssGpB0 Figure 1H ------- COMMENT: af542192349c135a 12 aOqYL7F2KV5d9E4mVC2uoiGmluk Figure 1E ------- COMMENT: af81fc769ebaf5b7 18 zuCctGUhhUzslANR+Fqa1FNR7zs (comment: yes it looks like pol II?! (val: changed to DNA binding term)) ------- COMMENT: af89930c3f9430a7 1 FDgOsHbxDozuTK4BDmOe8XScBhM All subunits in the Arp2/3 complex, except for Arp2 and ARPC5, contact the mother filament directly. ------- COMMENT: af89930c3f9430a7 2 FDgOsHbxDozuTK4BDmOe8XScBhM All subunits in the Arp2/3 complex, except for Arp2 and ARPC5, contact the mother filament directly. ------- COMMENT: af89930c3f9430a7 3 FDgOsHbxDozuTK4BDmOe8XScBhM All subunits in the Arp2/3 complex, except for Arp2 and ARPC5, contact the mother filament directly. ------- COMMENT: af89930c3f9430a7 4 FDgOsHbxDozuTK4BDmOe8XScBhM All subunits in the Arp2/3 complex, except for Arp2 and ARPC5, contact the mother filament directly. ------- COMMENT: af89930c3f9430a7 5 FDgOsHbxDozuTK4BDmOe8XScBhM All subunits in the Arp2/3 complex, except for Arp2 and ARPC5, contact the mother filament directly. ------- COMMENT: af89930c3f9430a7 6 FDgOsHbxDozuTK4BDmOe8XScBhM All subunits in the Arp2/3 complex, except for Arp2 and ARPC5, contact the mother filament directly. ------- COMMENT: af89930c3f9430a7 7 L5zpjatyLgF/KQCBRhTFtUafZt0 Cryo-EM structures reveal how phosphate release from Arp3 weakens actin filament branches formed by Arp2/3 comple ------- COMMENT: af89930c3f9430a7 8 L5zpjatyLgF/KQCBRhTFtUafZt0 Cryo-EM structures reveal how phosphate release from Arp3 weakens actin filament branches formed by Arp2/3 comple ------- COMMENT: af89930c3f9430a7 9 L5zpjatyLgF/KQCBRhTFtUafZt0 Cryo-EM structures reveal how phosphate release from Arp3 weakens actin filament branches formed by Arp2/3 comple ------- COMMENT: af89930c3f9430a7 10 L5zpjatyLgF/KQCBRhTFtUafZt0 Cryo-EM structures reveal how phosphate release from Arp3 weakens actin filament branches formed by Arp2/3 comple ------- COMMENT: af89930c3f9430a7 11 L5zpjatyLgF/KQCBRhTFtUafZt0 Cryo-EM structures reveal how phosphate release from Arp3 weakens actin filament branches formed by Arp2/3 comple ------- COMMENT: af89930c3f9430a7 12 L5zpjatyLgF/KQCBRhTFtUafZt0 Cryo-EM structures reveal how phosphate release from Arp3 weakens actin filament branches formed by Arp2/3 comple ------- COMMENT: af89930c3f9430a7 13 L5zpjatyLgF/KQCBRhTFtUafZt0 Cryo-EM structures reveal how phosphate release from Arp3 weakens actin filament branches formed by Arp2/3 comple ------- COMMENT: b015a7efb76f7cb7 4 V1icdZ5T6P2fJTpWsMZzXJnXjvg This demonstrates that the ‘‘activated’’ Spc7-9TE binding platform is sufficient to recruit these three checkpoint proteins constitutively, and that this works ectopically and thus does not require additional kinetochore factors. and Figure 1C. thus, we believe that this Spc7-Bub-Mad3 complex likely acts as an independent signaling module ------- COMMENT: b015a7efb76f7cb7 6 W2HhW5WM0PwvWfAQhblGQSo94KY Figures 1D and 1E / ectopic. show a very striking result: co-expression of TetR-Spc7-9TE with TetR-D(1-302)Mph1 was sufficient to arrest cells in mitosis. ------- COMMENT: b015a7efb76f7cb7 7 qEsVLtCQ2dcbMjDswUQJ9Q0n28o Spc7-wt arrested significantly faster than Spc7-9TE, with $60% mitotic arrest after 12 hr compared to 16 hr for Spc7-9TE. ------- COMMENT: b015a7efb76f7cb7 9 YdM3ZpApvcqPhYj2sgcTxd09Pgo Strains co-expressing Spc7 and Mph1 do not accumulate Mad2-GFP at spindle poles in strains containing the mad1-KAKA mutation that disrupts the Mad1-Cut7 kinesin motor interaction. ------- COMMENT: b015a7efb76f7cb7 10 D+VRJyxV2RBxh/xeW8Ubu+CCrek 136 amino acids of Mad1 containing a coiled-coil region (CC) were removed, preventing Mad1-Mad2 interaction with Mlps and the nuclear envelope and also removing the Cut7 interaction site. These mad1-DCC cells were also able to arrest efficiently when TetR-Spc7-9TE and TetR-D(1-302)Mph1 were co-expressed (Figure 3E). We conclude that the Mad and Bub proteins do not need to be enriched at kinetochores, spindle poles, or the nuclear periphery for a robust checkpoint arrest to be generated in fission yeast. Most likely a diffuse, soluble pool of Spc7 ------- COMMENT: b015a7efb76f7cb7 12 NXU5v665Yst2S+RcyVVu0+4sQ40 advance (by 4 hr) in the timing of arrest in bub3D cells arresting due to Spc7-9TE cells (although there is no effect with Spc7-wt, see Figure S4C). ------- COMMENT: b03ae5190e2fe612 2 3GL2WzMXr82AtBrgrUdJM0xrcjg higher protein level than in absence of HU, in both wild type and mutant ------- COMMENT: b04f65f05c1bf815 1 2RQsmpqKOcjSd5vbvMDjxEBXsik As shown in Figure 2a, cells overproducing Cut7 under the induced condition is lethal ------- COMMENT: b04f65f05c1bf815 2 vhqX8dhYTTNGTgM/fZeqR/hAdq8 We notified that a small percentage of either Eg5-NLS or Cut7 overproducing cells showed the monopolar spindle phenotype (Figure 2c; after 18 h, 10% for Cut7 vs 11% for Eg5-NLS). ------- COMMENT: b04f65f05c1bf815 3 WG344hRLREPEWcPgMmFuaI4fvcs Observation of spindle morphology showed that cells in which either Cut7 or Eg5-NLS is overproduced displayed the emergence of protruding spindles, which was never observed in the vector control strain. In these cells, the spindle MTs ex- tended away from one side or both sides of the spindle poles, in which the spindle pole bodies (SPBs, fungi equivalents of animal centrosomes) localized to the MT tips (Figure 2b). ------- COMMENT: b05124975291e7b8 1 gtpQJrKVE8fzxcgoPLFwNJ3D5HY figure 2. ------- COMMENT: b05124975291e7b8 3 gtpQJrKVE8fzxcgoPLFwNJ3D5HY figure 2. ------- COMMENT: b05124975291e7b8 5 gtpQJrKVE8fzxcgoPLFwNJ3D5HY figure 2. ------- COMMENT: b05124975291e7b8 8 gtpQJrKVE8fzxcgoPLFwNJ3D5HY figure 2. ------- COMMENT: b05124975291e7b8 9 gtpQJrKVE8fzxcgoPLFwNJ3D5HY figure 2. ------- COMMENT: b05124975291e7b8 10 gtpQJrKVE8fzxcgoPLFwNJ3D5HY figure 2. ------- COMMENT: b05124975291e7b8 11 gtpQJrKVE8fzxcgoPLFwNJ3D5HY figure 2. ------- COMMENT: b08eb33b3cfbe948 3 ZS4kltoUpYQ/1o9L3xz9DfMwhR4 (comment: looks very likely it is a ser/thr kinase, but if anything comes up that contradicts it this annotation can be made less specific) ------- COMMENT: b096f47215a08c2e 12 MOHdthDGv0fUdY8HGcwMOy5AVGw Fig. 5S ------- COMMENT: b096f47215a08c2e 18 39PrS/+0aee7NQmr6Kjzs1DD/Zc Fig. 5X ------- COMMENT: b096f47215a08c2e 19 jycMipWE0YoNxHicp0QZ9xu7a/4 (comment: all independent of Sty1 (effects of H2O2 & NAC unchanged in sty1delta)) ------- COMMENT: b10feb0836d1f5f7 1 Khp4emiB2X0MPc8gyDi+Qsr6ONo Fig. 2a ------- COMMENT: b10feb0836d1f5f7 2 +FlhNRJiKGt/yK6k7YgKvkUwGBU Fig. 2c, d).(comment: CHECK "decreased CENP-A maintenance during M-phase".) defective in CENP-A maintenance during M phase, as well as defective in CENP-A loading during interphase The GFP-Cnp1 intensity at centromeres decayed more rapidly in mis6-302 cells than in WT cells (Fig. 2f, g and Supplementary Figs. 4 and 5a). Taken together, these results suggest that Mis6, but not Scm3, is responsible for the maintenance of Cnp1 at centromeres during metaphase. ------- COMMENT: b10feb0836d1f5f7 3 Khp4emiB2X0MPc8gyDi+Qsr6ONo Fig. 2a ------- COMMENT: b10feb0836d1f5f7 6 8zL3TMcXCHa0+IhIMiHBbwHCcag Defective in CENP-A maintenance. Mis15 localises to the inner regions of centromeres as does Mis6, while Mis12 and Nuf2 localise to the outer regions relative to Mis6, and Mis6 localised to centromeres in the mis12 and nuf2 mutants but not in the mis15 mutant (Supplementary Fig. 6)In the mis15-68 mutants, signal intensities of Cnp1 at centromeres during mitotic arrest were decreased like in the mis6-302 mutant, whereas not in the mis12-537 and nuf2-2 mutants (Fig. 2h–j and Supplementary Figs. 5b–d and 7) ------- COMMENT: b10feb0836d1f5f7 7 wZCpt3ogWqZrRsau9v1KCZUh1ZE figure4 ------- COMMENT: b10feb0836d1f5f7 8 wZCpt3ogWqZrRsau9v1KCZUh1ZE figure4 ------- COMMENT: b10feb0836d1f5f7 9 OP3/htBvCHSsWyKY+K/MUp7clKc (comment: CHECK normal CENP-A maintenance) ------- COMMENT: b10feb0836d1f5f7 11 AHcqcS2pRZ0m57s/kQ8rgRH9NmU (comment: CHECK decreased CENP-A maintenance) ------- COMMENT: b10feb0836d1f5f7 13 OP3/htBvCHSsWyKY+K/MUp7clKc (comment: CHECK normal CENP-A maintenance) ------- COMMENT: b10feb0836d1f5f7 15 OP3/htBvCHSsWyKY+K/MUp7clKc (comment: CHECK normal CENP-A maintenance) ------- COMMENT: b10feb0836d1f5f7 16 OP3/htBvCHSsWyKY+K/MUp7clKc (comment: CHECK normal CENP-A maintenance) ------- COMMENT: b10feb0836d1f5f7 17 CWyG6bwZGfh7c7A61zKcwGmuQEo (comment: CHECK at high temperaturecut9 mis6 cut9-665(aaA535T) mis6-302(aaG135E)) ------- COMMENT: b10feb0836d1f5f7 18 mIix/9yU1MoVqPVYfNmwN5wJwIk at high temperatureLikewise, severe growth defects were observed in the cnp1-1 cut9- 665 double mutant, suggesting that retention of Cnp1 is required for passage through mitosis (Supplementary Fig. 2). ------- COMMENT: b10feb0836d1f5f7 20 kVhLraW3LN0QXDWObFbQiCnssDY Note that an increase of transcript levels from centromeres has not been demonstrated in the paper. What has been exactly demonstrated is an increased association of RNA polII to the cnt region of centromeres. ------- COMMENT: b10feb0836d1f5f7 21 kVhLraW3LN0QXDWObFbQiCnssDY Note that an increase of transcript levels from centromeres has not been demonstrated in the paper. What has been exactly demonstrated is an increased association of RNA polII to the cnt region of centromeres. ------- COMMENT: b10feb0836d1f5f7 23 5LwipdN5GVOSsh1t0s+a2bGVaeM Fig. 2a The resultant mis6-302 cut9-665 double mutant exhibited severe growth defects, even at the semi-restrictive temperature (Fig. 2a). This suggests that the mitotic function of Mis6 is crucial for prolonged metaphase. ------- COMMENT: b10feb0836d1f5f7 24 vaJ3dv5vxNXIRancJz/SqzflCrc Figure 2a (comment: CHECK during M-phase) ------- COMMENT: b10feb0836d1f5f7 25 ITwWdhwGaXj+/zOAtLyyC/r6Q6Q (comment: CHECK during M-phase) Mis15 localises to the inner regions of centromeres as does Mis6, while Mis12 and Nuf2 localise to the outer regions relative to Mis6, and Mis6 localised to centromeres in the mis12 and nuf2 mutants but not in the mis15 mutant (Supplementary Fig. 6) ------- COMMENT: b10feb0836d1f5f7 26 9st7alDQTumNiLLDDBJYgCse4yc during M-phase Mis15 localises to the inner regions of centromeres as does Mis6, while Mis12 and Nuf2 localise to the outer regions relative to Mis6, and Mis6 localised to centromeres in the mis12 and nuf2 mutants but not in the mis15 mutant (Supplementary Fig. 6) ------- COMMENT: b10feb0836d1f5f7 27 h0lRIbLJegnRa+NXxyaEyiNXo14 (comment: CHECK during M-phase) Supplementary Fig. 9b). These results demonstrate that kinetochore mutants with the intact inner kinetochore architecture retained the ability to silence transcrip- tion at the central core region. ------- COMMENT: b10feb0836d1f5f7 28 h0lRIbLJegnRa+NXxyaEyiNXo14 (comment: CHECK during M-phase) Supplementary Fig. 9b). These results demonstrate that kinetochore mutants with the intact inner kinetochore architecture retained the ability to silence transcrip- tion at the central core region. ------- COMMENT: b10feb0836d1f5f7 29 C4CyCwrRRr+2nUSM1vHYezqggpU When we followed the temporal kinetics of GFP- Cnp1 intensity during metaphase, the reduction of GFP-Cnp1 intensity seen in mis6-302 and mis15-68 cells was rescued by the additional knockout of Fft3 (mis6-302 fft3Δ, Supplementary Fig. 11a–c; mis15-68 fft3Δ, Supplementary Fig. 11d–f), confirming that Fft3 removes Cnp1 upon transcription at centromeres. ------- COMMENT: b10feb0836d1f5f7 33 Khp4emiB2X0MPc8gyDi+Qsr6ONo Fig. 2a ------- COMMENT: b13cc9bdeed9433e 1 hCNtTDzFH5b0lY7nxo50oHHxoaM Figure 3 summarizes data ------- COMMENT: b13cc9bdeed9433e 2 hCNtTDzFH5b0lY7nxo50oHHxoaM Figure 3 summarizes data ------- COMMENT: b13cc9bdeed9433e 3 hCNtTDzFH5b0lY7nxo50oHHxoaM Figure 3 summarizes data ------- COMMENT: b13cc9bdeed9433e 4 hCNtTDzFH5b0lY7nxo50oHHxoaM Figure 3 summarizes data ------- COMMENT: b13cc9bdeed9433e 5 hCNtTDzFH5b0lY7nxo50oHHxoaM Figure 3 summarizes data ------- COMMENT: b13cc9bdeed9433e 6 hCNtTDzFH5b0lY7nxo50oHHxoaM Figure 3 summarizes data ------- COMMENT: b13cc9bdeed9433e 7 hCNtTDzFH5b0lY7nxo50oHHxoaM Figure 3 summarizes data ------- COMMENT: b13cc9bdeed9433e 8 hCNtTDzFH5b0lY7nxo50oHHxoaM Figure 3 summarizes data ------- COMMENT: b13cc9bdeed9433e 9 hCNtTDzFH5b0lY7nxo50oHHxoaM Figure 3 summarizes data ------- COMMENT: b1564cf7a7073d6f 28 S6xJ6cvWyzrMLlfPAtiXZ0SG3gI (comment: assayed using artificial reporter construct ura4 containing two introns and one exon from nda3) ------- COMMENT: b1564cf7a7073d6f 29 S6xJ6cvWyzrMLlfPAtiXZ0SG3gI (comment: assayed using artificial reporter construct ura4 containing two introns and one exon from nda3) ------- COMMENT: b1564cf7a7073d6f 33 S6xJ6cvWyzrMLlfPAtiXZ0SG3gI (comment: assayed using artificial reporter construct ura4 containing two introns and one exon from nda3) ------- COMMENT: b1564cf7a7073d6f 34 S6xJ6cvWyzrMLlfPAtiXZ0SG3gI (comment: assayed using artificial reporter construct ura4 containing two introns and one exon from nda3) ------- COMMENT: b1769602f49f8e69 1 cajrtWpZ47REAXKqz9l5ZRypk4I A tas3 deletion strain carrying the ura4+ reporter gene inserted at innermost (imr) or outermost (otr) centromeric repeats of chromosome 1 (imr1R::ura4+ and otr1R::ura4+, respectively) displayed a loss of silencing of both reporter genes (Fig. 2D) to an extent similar to that of the deletion of sir2, chp1, or ago1 (Fig. 2D) ------- COMMENT: b1769602f49f8e69 2 cajrtWpZ47REAXKqz9l5ZRypk4I A tas3 deletion strain carrying the ura4+ reporter gene inserted at innermost (imr) or outermost (otr) centromeric repeats of chromosome 1 (imr1R::ura4+ and otr1R::ura4+, respectively) displayed a loss of silencing of both reporter genes (Fig. 2D) to an extent similar to that of the deletion of sir2, chp1, or ago1 (Fig. 2D) ------- COMMENT: b1769602f49f8e69 3 cajrtWpZ47REAXKqz9l5ZRypk4I A tas3 deletion strain carrying the ura4+ reporter gene inserted at innermost (imr) or outermost (otr) centromeric repeats of chromosome 1 (imr1R::ura4+ and otr1R::ura4+, respectively) displayed a loss of silencing of both reporter genes (Fig. 2D) to an extent similar to that of the deletion of sir2, chp1, or ago1 (Fig. 2D) ------- COMMENT: b1769602f49f8e69 4 cajrtWpZ47REAXKqz9l5ZRypk4I A tas3 deletion strain carrying the ura4+ reporter gene inserted at innermost (imr) or outermost (otr) centromeric repeats of chromosome 1 (imr1R::ura4+ and otr1R::ura4+, respectively) displayed a loss of silencing of both reporter genes (Fig. 2D) to an extent similar to that of the deletion of sir2, chp1, or ago1 (Fig. 2D) ------- COMMENT: b1769602f49f8e69 5 O1ZDylq6CDRTCaaAq2G0yPsAt/8 Further, chromatin immunoprecipitation (ChIP) showed that Tas3 was required for H3-K9 methylation and Swi6 localization of a ura4+ reporter gene inserted at each of the above loci (Fig. 2E). ------- COMMENT: b1769602f49f8e69 7 B9ztCYX2jRj7+uzSNZU1pc1dOqY Furthermore, deletion of ago1+, dcr1+, or rdp1+ abolished the association of Chp1-Flag and Tas3-TAP with otr1::ura4+, as well as with centromeric repeat sequences (Fig. 4, A and B). ------- COMMENT: b1769602f49f8e69 8 B9ztCYX2jRj7+uzSNZU1pc1dOqY Furthermore, deletion of ago1+, dcr1+, or rdp1+ abolished the association of Chp1-Flag and Tas3-TAP with otr1::ura4+, as well as with centromeric repeat sequences (Fig. 4, A and B). ------- COMMENT: b1769602f49f8e69 9 B9ztCYX2jRj7+uzSNZU1pc1dOqY Furthermore, deletion of ago1+, dcr1+, or rdp1+ abolished the association of Chp1-Flag and Tas3-TAP with otr1::ura4+, as well as with centromeric repeat sequences (Fig. 4, A and B). ------- COMMENT: b1769602f49f8e69 10 U24ICplwo/0xoCTkqfWh2BWU2GI Deletion of tas3+ abolished the association of Chp1-Flag with otr1::ura4+, as well as with native cen sequences (Fig. 4C). ------- COMMENT: b19dfb2b27f82b53 2 wj2VSE893AMBlEWR8+9Ey55om+8 Fig 2B ------- COMMENT: b19dfb2b27f82b53 4 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: b19dfb2b27f82b53 5 6pSAHAPl4EEMTDqkfHAb9E42zmg Fig 2D ------- COMMENT: b19dfb2b27f82b53 6 rWXQyfHMYdVvp2i/3LLWEZKWECw Data was not shown. ------- COMMENT: b19dfb2b27f82b53 7 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: b19dfb2b27f82b53 8 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: b19dfb2b27f82b53 9 MbiHgaeQZ2Y5UK1ngqO4eZDxyO4 (comment: forms microcolonies) ------- COMMENT: b19dfb2b27f82b53 10 2tpJ2pdSU1k53FeiX74cgkWhvbE Fig 2G ------- COMMENT: b19dfb2b27f82b53 13 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: b19dfb2b27f82b53 14 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: b19dfb2b27f82b53 15 EHbNKpere9ZcTxk42WgxvY3v9H0 Fig 6 cell tip localisation increased compared to exponentially growing cells ------- COMMENT: b19dfb2b27f82b53 16 Q6KgDi1BNf7h0RRRbd6ML5GgVcw Fig 8 and Fig 5 Tea2 is not completely delocalised but is has a more extended distribution along the microtubules ------- COMMENT: b19dfb2b27f82b53 17 5yBZEPhM4GROV0qZh5yj32Jj1tA Fig 8 (comment: vw: I made this 'along micriotubule because we know its microtubule dept) ------- COMMENT: b19dfb2b27f82b53 18 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: b19dfb2b27f82b53 19 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 20 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 21 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 22 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 23 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 24 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 25 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 26 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 27 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 28 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 29 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 30 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 31 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 32 LWXBIdG1CfIbil3yMInWM+hk0BY growth assayed on agar plates at different temperature and media. ------- COMMENT: b19dfb2b27f82b53 34 5Zu3evtKO3tOGM84AIBzY5G4/OA fig 8c ------- COMMENT: b19dfb2b27f82b53 36 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 37 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 38 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 39 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 40 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 41 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 42 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 43 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 44 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 45 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 46 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 47 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 48 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 49 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 50 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 51 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 52 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 53 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 54 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 55 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 56 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 57 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 58 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 59 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 60 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 61 ZMnl4SdO3r4g+D1pXGmQevdw++0 growth assayed on agar plates at different temperature and media ------- COMMENT: b19dfb2b27f82b53 62 LWXBIdG1CfIbil3yMInWM+hk0BY growth assayed on agar plates at different temperature and media. ------- COMMENT: b19dfb2b27f82b53 63 LWXBIdG1CfIbil3yMInWM+hk0BY growth assayed on agar plates at different temperature and media. ------- COMMENT: b1bc10e390be46c7 1 Qy/y/ynfWxhe5XE/zYhGMkeDR2A (comment: CHECK synthetic lethality with sad1.2 mutant allele) ------- COMMENT: b1bc10e390be46c7 5 kktWx+HWH+Dn4dEAYAXZnINR3Rw (comment: late spindle elongation (move down /when GO reflect stages of meiotic spindle elongation)) ------- COMMENT: b1bc10e390be46c7 7 2J9D8Gu1gJ1l1r77W0bxz1Wf8bc Figure 2F ------- COMMENT: b1bc10e390be46c7 8 5Leuj0nQtQ/oH58v8XLkYXSDMog Figure 6B ------- COMMENT: b1bc10e390be46c7 19 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: b1bc10e390be46c7 20 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: b1bc10e390be46c7 28 2J9D8Gu1gJ1l1r77W0bxz1Wf8bc Figure 2F ------- COMMENT: b1bc10e390be46c7 29 iw2OU4q9pbz6nlQAA2v4bdMWaVg Figure 6 Remarkably, we found that, in the case of bqt1Δ sad1.2 alp14-26 meiocytes, despite clear dysfunction of alp14-26, self-assembled spindles were still able to form and behaved normally (Fig. 6C,D). However, they formed in a smaller percentage of meiocytes (from ∼80% to ∼30%, Fig. 6F), indicating that the contribution of Alp14 to self-assembled spindle formation and behaviour is substantial. ------- COMMENT: b1bc10e390be46c7 30 I7Y3E0LKKeNIXqOZ/a8XKWV+bfY analysis of bqt1Δ sad1.2 dis1Δ meiocytes showed that the percentage of selfassembled spindles with normal formation and function was similar to that in the bqt1Δ sad1.2 setting (Fig. 6F,H). ------- COMMENT: b1bc10e390be46c7 32 vyXnMvGrlaEE+BSyCbRlvSKpcLw figure7 maximum length of self-assembled spindles increased upon deletion of klp6 (from 7.9±3.8 μm to 12.9±4.7 μm; Fig. 7E). ------- COMMENT: b1bc10e390be46c7 33 1ftlqC3vGOhSAPfzae7f1+hJ9Do figure7C ------- COMMENT: b1bc10e390be46c7 34 53f2f49HV6z8wbV/EKJdxBf0yso (comment: CHECK decreased abnormal SPB-independent meiosis II) ------- COMMENT: b1bc10e390be46c7 35 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: b1bc10e390be46c7 36 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: b1bc10e390be46c7 41 F1uyVJcytgLEqb7EuoI9n9nPvLo Figure 5 To confirm this, we evaluatedthe behaviour of bqt1Δ sad1.2 alp4-GFP cells harbouring the SPBmarkers Sid4–mCherry and Sad1.2–mCherry, effectively showingthat Alp4–GFP molecules located far from the nucleus wereassociated with the SPBs (Fig. 5C,D) ------- COMMENT: b1bc10e390be46c7 44 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: b1bc10e390be46c7 45 FHJoZi236WuBW6DtFtvqk6h9sZk Figure 1 (comment: see above) ------- COMMENT: b1bc10e390be46c7 47 FHJoZi236WuBW6DtFtvqk6h9sZk Figure 1 (comment: see above) ------- COMMENT: b1bc10e390be46c7 48 /YjgqxTXk0IGbMcXyFsAA8D/074 Figure 2E ------- COMMENT: b1bc10e390be46c7 49 /YjgqxTXk0IGbMcXyFsAA8D/074 Figure 2E ------- COMMENT: b1bc10e390be46c7 50 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: b1bc10e390be46c7 52 vyXnMvGrlaEE+BSyCbRlvSKpcLw figure7 maximum length of self-assembled spindles increased upon deletion of klp6 (from 7.9±3.8 μm to 12.9±4.7 μm; Fig. 7E). ------- COMMENT: b1bc10e390be46c7 53 F1uyVJcytgLEqb7EuoI9n9nPvLo Figure 5 To confirm this, we evaluatedthe behaviour of bqt1Δ sad1.2 alp4-GFP cells harbouring the SPBmarkers Sid4–mCherry and Sad1.2–mCherry, effectively showingthat Alp4–GFP molecules located far from the nucleus wereassociated with the SPBs (Fig. 5C,D) ------- COMMENT: b1bc10e390be46c7 54 2J9D8Gu1gJ1l1r77W0bxz1Wf8bc Figure 2F ------- COMMENT: b1bc10e390be46c7 56 iTCvWlQsUPnD5CUXX4rd8lac4UQ figure 7C ------- COMMENT: b1bc10e390be46c7 58 iTCvWlQsUPnD5CUXX4rd8lac4UQ figure 7C ------- COMMENT: b1f93197a5f7b23e 5 82TtXx/roEvsAXHvA1wJG79SqgQ Fig. 3D and data not shown ------- COMMENT: b1f93197a5f7b23e 7 2He3fSKvXpS3YzqmKt6gbioa8J8 Table2 ------- COMMENT: b1f93197a5f7b23e 8 2He3fSKvXpS3YzqmKt6gbioa8J8 Table2 ------- COMMENT: b2192f51379c99f3 1 xY4XfsxNNk10GYzsXRrFjASwL38 (comment: Beware using aged colonies, cell size recovery observed.) ------- COMMENT: b2192f51379c99f3 8 rpkiQvGo2rounn5q4W54VSbqk54 (comment: beware using old strains, phenotypic changes observed.) ------- COMMENT: b22f1157ab22d428 38 hCKPq5n/loQHiQGtrmukJoxRSJY (comment: in response to cytokinesis after mitosis checkpoint) ------- COMMENT: b237acc888396401 33 /UTPGIw8lWoNkVl/QnR+7PJyibA (comment: CHECK CCU codon/AGG anticodon tRNA) ------- COMMENT: b237acc888396401 34 x8yf3Acm/QxF8CLYtavZAUL7NEA (comment: CHECK UAC codon/GUA anticodon tRNA) ------- COMMENT: b237acc888396401 35 U+9TzQuZqs8XoyRaNNTChrCZSb0 (comment: CHECK ACU codon/AGU anticodon tRNA) ------- COMMENT: b237acc888396401 36 /UTPGIw8lWoNkVl/QnR+7PJyibA (comment: CHECK CCU codon/AGG anticodon tRNA) ------- COMMENT: b237acc888396401 37 x8yf3Acm/QxF8CLYtavZAUL7NEA (comment: CHECK UAC codon/GUA anticodon tRNA) ------- COMMENT: b237acc888396401 38 /UTPGIw8lWoNkVl/QnR+7PJyibA (comment: CHECK CCU codon/AGG anticodon tRNA) ------- COMMENT: b237acc888396401 39 x8yf3Acm/QxF8CLYtavZAUL7NEA (comment: CHECK UAC codon/GUA anticodon tRNA) ------- COMMENT: b237acc888396401 40 /UTPGIw8lWoNkVl/QnR+7PJyibA (comment: CHECK CCU codon/AGG anticodon tRNA) ------- COMMENT: b237acc888396401 41 x8yf3Acm/QxF8CLYtavZAUL7NEA (comment: CHECK UAC codon/GUA anticodon tRNA) ------- COMMENT: b237acc888396401 42 /UTPGIw8lWoNkVl/QnR+7PJyibA (comment: CHECK CCU codon/AGG anticodon tRNA) ------- COMMENT: b237acc888396401 43 x8yf3Acm/QxF8CLYtavZAUL7NEA (comment: CHECK UAC codon/GUA anticodon tRNA) ------- COMMENT: b237acc888396401 44 /UTPGIw8lWoNkVl/QnR+7PJyibA (comment: CHECK CCU codon/AGG anticodon tRNA) ------- COMMENT: b237acc888396401 45 x8yf3Acm/QxF8CLYtavZAUL7NEA (comment: CHECK UAC codon/GUA anticodon tRNA) ------- COMMENT: b27ca821f4f21986 4 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: b27ca821f4f21986 5 DfWj/8L6P9DMkfzrj24T0Mkh3oo fig 2a For example, in the second cell cycle, Wee1 phosphorylation was initiated at the end of G2 (180 minutes) and reached a maximum level at metaphase (200 minutes), just before Cdc13 degradation in anaphase (220 minutes). ------- COMMENT: b27ca821f4f21986 6 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: b27ca821f4f21986 7 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: b27ca821f4f21986 9 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: b27ca821f4f21986 10 UBFGWG/1GVmzWcA3dQF2KZT4MO4 fig3a (comment: Cdk1 consensus sites) ------- COMMENT: b27ca821f4f21986 12 z59+Q/ZB/WS4n0ShzC3Ivt0Nj2Q changes in phosphrylation level fig4 Wee1 to remain in the partially phosphorylated form throughout the cell cycle (Fig. S2) ------- COMMENT: b27ca821f4f21986 13 z59+Q/ZB/WS4n0ShzC3Ivt0Nj2Q changes in phosphrylation level fig4 Wee1 to remain in the partially phosphorylated form throughout the cell cycle (Fig. S2) ------- COMMENT: b27ca821f4f21986 17 bJaKacHg+Y/T8v0A02Nd4/gEnCs The cell cycle was 20 min longer in clp1D cells compared with wild type cells. ------- COMMENT: b27ca821f4f21986 18 c0WjGyP4zJdg3ipjYOK2wm1IHLw changes in phosphorylation level Figs. 5A, B and S2) ------- COMMENT: b27ca821f4f21986 19 c0WjGyP4zJdg3ipjYOK2wm1IHLw changes in phosphorylation level Figs. 5A, B and S2) ------- COMMENT: b27ca821f4f21986 20 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: b27ca821f4f21986 21 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: b284d3aacd905379 3 47F1zY8um7KjNDYPymJO+C0fuxY (comment: CHECK in asf1-33 at higher temperature) ------- COMMENT: b284d3aacd905379 32 0Ignyum22RxvN1nFczB37cs8CPg (comment: CHECK mild expressivity) ------- COMMENT: b2a1e8f079c67607 2 Tgwcw/Hq/ZvZ42Dhf3L52BySLu8 (comment: CHECK abolished) figure 1E ------- COMMENT: b2a1e8f079c67607 3 Tgwcw/Hq/ZvZ42Dhf3L52BySLu8 (comment: CHECK abolished) figure 1E ------- COMMENT: b2a1e8f079c67607 4 F4ltNRjXsjaLeGz+DafmDqxOoZE figure 1j ------- COMMENT: b2ae716b0ad7c3cb 1 923NdDFpSX0NocE3j/423lZ2IXs (comment: type 2 cohesion (still bound) MITOTIC) ------- COMMENT: b2ae716b0ad7c3cb 2 bbkOFwullzevIr15GkPOR3mM1c8 (comment: MIOTOTIC) ------- COMMENT: b2ae716b0ad7c3cb 4 t6uyTtt4P/8kRlIjC2qelSNbzfs Fig 3C ------- COMMENT: b2ae716b0ad7c3cb 5 hrcIGlkP/sem5Q9C7kUi/e7UA4w Fig EV5 ------- COMMENT: b2ae716b0ad7c3cb 6 8Bfe9Cak+Lc8NE4KYnce9AOU+B8 Fig 4E ------- COMMENT: b2ae716b0ad7c3cb 7 1sT/IV/MBkW8mAcd7yAyYinHfUM Figure 5 B (comment: exacerbates ) ------- COMMENT: b2ae716b0ad7c3cb 8 VFkskau1qxV6L9SVWfIqbfw500g Fig 5A ------- COMMENT: b2ae716b0ad7c3cb 9 oHnt+5FCXDoteQ7fvGmLa4TzUcA (comment: required for Rad21 dephosphorylation) ------- COMMENT: b2ae716b0ad7c3cb 10 /P0jfJAN2NT3cj+5oi/odCzWsS4 (comment: type 2 cohesion (still bound)) ------- COMMENT: b2ae716b0ad7c3cb 11 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: b2ae716b0ad7c3cb 12 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: b2ae716b0ad7c3cb 13 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: b2ae716b0ad7c3cb 14 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: b2ae716b0ad7c3cb 15 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: b2ae716b0ad7c3cb 16 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: b2ae716b0ad7c3cb 17 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: b2ae716b0ad7c3cb 18 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: b2ae716b0ad7c3cb 19 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: b2ae716b0ad7c3cb 20 QxyA7Kxt4u0SZftovXPYBddoxhU Fig 1D ------- COMMENT: b2ae716b0ad7c3cb 21 QxyA7Kxt4u0SZftovXPYBddoxhU Fig 1D ------- COMMENT: b2ae716b0ad7c3cb 22 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: b2ae716b0ad7c3cb 23 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: b2ae716b0ad7c3cb 24 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: b2ae716b0ad7c3cb 30 t6uyTtt4P/8kRlIjC2qelSNbzfs Fig 3C ------- COMMENT: b2ae716b0ad7c3cb 31 t6uyTtt4P/8kRlIjC2qelSNbzfs Fig 3C ------- COMMENT: b2ae716b0ad7c3cb 32 t6uyTtt4P/8kRlIjC2qelSNbzfs Fig 3C ------- COMMENT: b2ae716b0ad7c3cb 33 t6uyTtt4P/8kRlIjC2qelSNbzfs Fig 3C ------- COMMENT: b2ae716b0ad7c3cb 34 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: b2ae716b0ad7c3cb 37 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: b2b583def5375c2d 2 L3rvaFYVoIGOhexXsmPqTQic718 Figure S1. In contrast, 50% of the spindles in klp2D cells underwent abrupt collapse during metaphase I (Fig. 2, A, C, and E, 3A) ------- COMMENT: b2b583def5375c2d 3 3Tgm9Duz+4JAWRiMwV/1F7+1bN0 Figure S1. ------- COMMENT: b2b583def5375c2d 4 3Tgm9Duz+4JAWRiMwV/1F7+1bN0 Figure S1. ------- COMMENT: b2b583def5375c2d 5 3Tgm9Duz+4JAWRiMwV/1F7+1bN0 Figure S1. ------- COMMENT: b2b583def5375c2d 6 jbtkjlSqVahxl6U7PUn2gXwV57w absence of Klp2 did not significantly affect spindle elongation during prophase I and only slightly lengthened the maximal spindle length during metaphase I (Fig. 2G). ------- COMMENT: b2b583def5375c2d 7 YEL3JQLDuKk098eM+bNKwEnWLI8 In both mitotic and meiotic cells, the absence of Klp2 slightly but significantly prolonged the duration of preanaphase (Fig. 2, F and G). ------- COMMENT: b2b583def5375c2d 8 djy5q6zoRqvW1iT1IGQT5zpOB2M Fig. 3F. The results show that the absence of Klp2 affects the localization of Ase1-GFP to the meiotic spindles and leads to a decrease of the Ase1-GFP intensity on the meiotic spindles. ------- COMMENT: b2b583def5375c2d 10 L3rvaFYVoIGOhexXsmPqTQic718 Figure S1. In contrast, 50% of the spindles in klp2D cells underwent abrupt collapse during metaphase I (Fig. 2, A, C, and E, 3A) ------- COMMENT: b2b583def5375c2d 11 J2u7wgpFLjvg7fRGbeL4rb3g7iQ (comment: spindle phenotype rescued) ------- COMMENT: b2b583def5375c2d 12 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: b2b583def5375c2d 13 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: b2b583def5375c2d 14 iaOX/GGexyvuEuRsqkKrS1LG5Zs Figure 5 Three typical types of plots are shown: (I) WTlike metaphase spindle length (maintenance), (II) spindle regression (regression), and (III) continuous spindle elongation (lacking metaphase). ------- COMMENT: b2b583def5375c2d 15 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: b2b583def5375c2d 18 4S49bQb4H99N8Jj+qilD7HGsICU Ase1 is required for promoting spindle elongation during mitotic prophase but synergizes with Klp2 to maintain spindle stability during metaphase I. ------- COMMENT: b2b583def5375c2d 19 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: b2b583def5375c2d 20 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: b2b583def5375c2d 21 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: b2b583def5375c2d 23 4S49bQb4H99N8Jj+qilD7HGsICU Ase1 is required for promoting spindle elongation during mitotic prophase but synergizes with Klp2 to maintain spindle stability during metaphase I. ------- COMMENT: b2b583def5375c2d 24 4S49bQb4H99N8Jj+qilD7HGsICU Ase1 is required for promoting spindle elongation during mitotic prophase but synergizes with Klp2 to maintain spindle stability during metaphase I. ------- COMMENT: b2b583def5375c2d 25 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: b2b583def5375c2d 26 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: b2b583def5375c2d 27 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: b2b583def5375c2d 28 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: b2b583def5375c2d 29 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: b2d538188151fdf1 117 RxZYBqNcJALANa9EGbo2ndT4QKw (comment: CHECK aerobic conditions) ------- COMMENT: b2d538188151fdf1 119 HuftyHK2xTCZDfTbtodvuC6eYu0 (comment: genes specified in extensions assayed in low-throughput Northern blots; additional genes assayed in high-throughput microarrays not listed) ------- COMMENT: b2f2007279faafbc 1 Yqgkr/1NT1BhwdhpnPkK4sXr/XA Fig. 2, Fig. 4A, Fig. S3A ------- COMMENT: b2f2007279faafbc 2 Yqgkr/1NT1BhwdhpnPkK4sXr/XA Fig. 2, Fig. 4A, Fig. S3A ------- COMMENT: b2f2007279faafbc 3 Yqgkr/1NT1BhwdhpnPkK4sXr/XA Fig. 2, Fig. 4A, Fig. S3A ------- COMMENT: b2f2007279faafbc 4 Yqgkr/1NT1BhwdhpnPkK4sXr/XA Fig. 2, Fig. 4A, Fig. S3A ------- COMMENT: b2f2007279faafbc 5 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: b2f2007279faafbc 6 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: b2f2007279faafbc 7 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: b2f2007279faafbc 8 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: b2f2007279faafbc 9 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: b2f2007279faafbc 10 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: b2f2007279faafbc 11 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: b2f2007279faafbc 12 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: b2f2007279faafbc 13 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: b2f2007279faafbc 14 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: b2f2007279faafbc 15 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: b2f2007279faafbc 16 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: b2f2007279faafbc 17 1lWSey39zXvAVXx+v+Yrn0KbLwQ Fig. 4A, Fig. S3A ------- COMMENT: b2f2007279faafbc 18 1lWSey39zXvAVXx+v+Yrn0KbLwQ Fig. 4A, Fig. S3A ------- COMMENT: b2f2007279faafbc 19 1lWSey39zXvAVXx+v+Yrn0KbLwQ Fig. 4A, Fig. S3A ------- COMMENT: b2f2007279faafbc 20 1lWSey39zXvAVXx+v+Yrn0KbLwQ Fig. 4A, Fig. S3A ------- COMMENT: b2f2007279faafbc 21 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: b2f2007279faafbc 22 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: b2f2007279faafbc 23 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: b2f2007279faafbc 24 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: b2f2007279faafbc 25 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: b2f2007279faafbc 26 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: b2f2007279faafbc 27 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: b2f2007279faafbc 28 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: b2f2007279faafbc 29 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: b2f2007279faafbc 30 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: b2f2007279faafbc 31 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: b2f2007279faafbc 32 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: b2f2007279faafbc 33 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: b2f2007279faafbc 34 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: b2f2007279faafbc 35 zZfEYh6YggqPbkaVD2H4rShmXMQ Fig. 4C, Fig. S3B ------- COMMENT: b2f2007279faafbc 36 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: b2f2007279faafbc 37 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: b2f2007279faafbc 38 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: b2f2007279faafbc 39 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: b2f2007279faafbc 40 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: b2f2007279faafbc 41 HELU/Ewr3/ldjjzKNomtwxDJp3o Fig. 4C, S3B ------- COMMENT: b2f2007279faafbc 42 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: b2f2007279faafbc 43 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: b2f2007279faafbc 44 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: b2f2007279faafbc 45 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: b2f2007279faafbc 46 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: b2f2007279faafbc 47 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: b2f2007279faafbc 48 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: b2f2007279faafbc 49 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: b2f2007279faafbc 50 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: b2f2007279faafbc 51 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: b2f2007279faafbc 52 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: b2f2007279faafbc 53 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: b2f2007279faafbc 54 srU5Cz/c8DGYwy3HMdKdATPxdZQ Fig. 6A, S3A ------- COMMENT: b2f2007279faafbc 55 srU5Cz/c8DGYwy3HMdKdATPxdZQ Fig. 6A, S3A ------- COMMENT: b2f2007279faafbc 56 srU5Cz/c8DGYwy3HMdKdATPxdZQ Fig. 6A, S3A ------- COMMENT: b2f2007279faafbc 57 srU5Cz/c8DGYwy3HMdKdATPxdZQ Fig. 6A, S3A ------- COMMENT: b2f2007279faafbc 58 srU5Cz/c8DGYwy3HMdKdATPxdZQ Fig. 6A, S3A ------- COMMENT: b2f2007279faafbc 59 srU5Cz/c8DGYwy3HMdKdATPxdZQ Fig. 6A, S3A ------- COMMENT: b2f2007279faafbc 60 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: b2f2007279faafbc 61 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: b2f2007279faafbc 62 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: b2f2007279faafbc 63 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: b2f2007279faafbc 64 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: b2f2007279faafbc 65 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: b2f2007279faafbc 66 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: b2f2007279faafbc 67 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: b2f2007279faafbc 69 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: b2f2007279faafbc 70 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: b2f2007279faafbc 71 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: b2f2007279faafbc 72 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: b2f2007279faafbc 73 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: b2f2007279faafbc 74 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: b2f2007279faafbc 75 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: b2f2007279faafbc 76 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: b2f2007279faafbc 78 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: b2f2007279faafbc 79 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: b2f2007279faafbc 80 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: b2f2007279faafbc 82 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: b2f2007279faafbc 83 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: b2f2007279faafbc 85 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: b2f2007279faafbc 86 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: b2f2007279faafbc 88 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: b2f2007279faafbc 89 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: b2f2007279faafbc 90 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: b2f2007279faafbc 91 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: b2f2007279faafbc 92 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: b2f2007279faafbc 93 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: b2f2007279faafbc 94 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: b2f2007279faafbc 95 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: b2f2007279faafbc 96 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: b2f2007279faafbc 97 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: b2f2007279faafbc 98 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: b2f2007279faafbc 99 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: b2f2007279faafbc 100 /voYlxikkjrZ4fz7XEUTVkV+ubc Fig. S3B ------- COMMENT: b2f2007279faafbc 101 Xb1L1D0oeozHWjRnmVjhdBSDo98 Fig. S3A ------- COMMENT: b2f2007279faafbc 102 Xb1L1D0oeozHWjRnmVjhdBSDo98 Fig. S3A ------- COMMENT: b2f2007279faafbc 103 Xb1L1D0oeozHWjRnmVjhdBSDo98 Fig. S3A ------- COMMENT: b2f2007279faafbc 104 /voYlxikkjrZ4fz7XEUTVkV+ubc Fig. S3B ------- COMMENT: b2f2007279faafbc 105 Xb1L1D0oeozHWjRnmVjhdBSDo98 Fig. S3A ------- COMMENT: b2f2007279faafbc 106 Xb1L1D0oeozHWjRnmVjhdBSDo98 Fig. S3A ------- COMMENT: b2f2007279faafbc 107 /voYlxikkjrZ4fz7XEUTVkV+ubc Fig. S3B ------- COMMENT: b2f2007279faafbc 108 Xb1L1D0oeozHWjRnmVjhdBSDo98 Fig. S3A ------- COMMENT: b2f2007279faafbc 109 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: b2f2007279faafbc 110 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: b2f2007279faafbc 132 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: b2f2007279faafbc 133 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: b2f2007279faafbc 134 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: b31214a9635efb8b 1 3yqq8mT6r2p3L3RL/sFe2Tak0os fig2A ------- COMMENT: b31214a9635efb8b 2 boS2RZga8c2ZZ1Xt2iNB5Prf2xk fig 2A (comment: CHECK abolished entry into meiosis (at pre meiosis?)) ------- COMMENT: b31214a9635efb8b 3 boS2RZga8c2ZZ1Xt2iNB5Prf2xk fig 2A (comment: CHECK abolished entry into meiosis (at pre meiosis?)) ------- COMMENT: b31214a9635efb8b 4 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: b31214a9635efb8b 5 1UwzEh35T+2C17+Fg9fWq7GonxY fig2 (comment: abolished asci formation) ------- COMMENT: b31214a9635efb8b 22 yn5/s6S+P83NU/sZm1yJpcjeWbk fig 3D ------- COMMENT: b31214a9635efb8b 23 yn5/s6S+P83NU/sZm1yJpcjeWbk fig 3D ------- COMMENT: b31214a9635efb8b 24 k2bW460MXhcptMmYUvt2sYQilh0 Figure 3F ------- COMMENT: b31214a9635efb8b 25 BBbwgZvxTtA0M6hI5zzMcf3sWZM Figure S3C-E ------- COMMENT: b31214a9635efb8b 26 BBbwgZvxTtA0M6hI5zzMcf3sWZM Figure S3C-E ------- COMMENT: b31214a9635efb8b 27 Z2yOrcpEIlpzYA+Sms+j1sa65pE Figure 4A and B:(comment: MI NDJ) ------- COMMENT: b31214a9635efb8b 28 Z2yOrcpEIlpzYA+Sms+j1sa65pE Figure 4A and B: (comment: MI NDJ) ------- COMMENT: b31214a9635efb8b 29 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: b31214a9635efb8b 31 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: b31214a9635efb8b 32 +8KlaMrbC9Ein2bFXjX3ccRmUQw fig 5D ------- COMMENT: b31214a9635efb8b 33 YcKUOPJq7WddihxWQBl5g6iZ1NI fig S5A ------- COMMENT: b31214a9635efb8b 34 d2BxjRAHnCOkutgYAosRtE51L8w fig 5E ------- COMMENT: b31214a9635efb8b 35 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: b31214a9635efb8b 39 djJSnNKEyGRVc/uSftKkGFeuo78 fig 7D, E, F ------- COMMENT: b31214a9635efb8b 41 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: b33dcb999a87be71 7 EaJ1cIJCgxH1h7dmT6mEA3YTf4A Fig. 1d ------- COMMENT: b33dcb999a87be71 14 dajcciCYqdPikaDIY5du9S3TcFs (comment: asynchronous) fig 3a ------- COMMENT: b33dcb999a87be71 15 dajcciCYqdPikaDIY5du9S3TcFs (comment: asynchronous) fig 3a ------- COMMENT: b33dcb999a87be71 16 dajcciCYqdPikaDIY5du9S3TcFs (comment: asynchronous) fig 3a ------- COMMENT: b33dcb999a87be71 25 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: b34dc47eaa1f4290 2 OYneNPAZnZ/SRWOVAdFyLrFFQLY inviable at 37 degrees; some growth at 34 degrees ------- COMMENT: b34dc47eaa1f4290 7 OYneNPAZnZ/SRWOVAdFyLrFFQLY inviable at 37 degrees; some growth at 34 degrees ------- COMMENT: b34dc47eaa1f4290 9 6ncz5cAlfYX0pnX1URxHjVAlY4I inviable at 34 or 37 degrees ------- COMMENT: b34dc47eaa1f4290 29 nB+UD++wy796pXhLsB5TJqZRJIQ (comment: temperature restrictive for cdc4-8 alone) ------- COMMENT: b34dc47eaa1f4290 30 nB+UD++wy796pXhLsB5TJqZRJIQ (comment: temperature restrictive for cdc4-8 alone) ------- COMMENT: b34dc47eaa1f4290 31 nB+UD++wy796pXhLsB5TJqZRJIQ (comment: temperature restrictive for cdc4-8 alone) ------- COMMENT: b34dc47eaa1f4290 32 nB+UD++wy796pXhLsB5TJqZRJIQ (comment: temperature restrictive for cdc4-8 alone) ------- COMMENT: b34dc47eaa1f4290 34 goEO9S3UHQnaYolcF2Vye6cN6UI (comment: dependent on F-actin (assayed using Latrunculin A)) ------- COMMENT: b34dc47eaa1f4290 37 RP6ow2YRwh5qZ0KQQhQK/T+JPqM (comment: early mitosis; independent of F-actin (assayed using Latrunculin A)) ------- COMMENT: b365bd06b75ce0d4 1 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: b365bd06b75ce0d4 2 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: b365bd06b75ce0d4 7 GzQQRIirPoWD2NK+XSQSE9jSc3U (comment: CHECK in vitro) ------- COMMENT: b3bc9cbfdcacc24c 1 Qg1uhYuICD64KLv3ekDVW/TAmyA Fig 1B,C demonstrates in vitro kinase activity. 2A in vivo ------- COMMENT: b3bc9cbfdcacc24c 2 LAxbElHsMEmkG3GLvFC8ueKlaGE Fig1 GST tea1 directly phosphorylated by Shk1 in vitro Fig2A GST-tea1 is phosphorylated in vivo in a Shk1 dependent manner ------- COMMENT: b3bc9cbfdcacc24c 3 o7eeDNtNzfw5c2GBLHCTgsVPyNc Fig2B. shk1 K415R mutant is expressed from a weak allele of nmt1 promoter ON but does not say whether it is expressed at wild type levels. pREP4XGST-tea1 is a multi copy plasmid promoter ON ------- COMMENT: b3bc9cbfdcacc24c 4 bkLNlv1zQFnqkUEMlRvfQTyu5Ps Fig3A tea1 delta is a temperature dependent suppressor of loss of skb15 ------- COMMENT: b3bc9cbfdcacc24c 5 yciAFstyWPrO4Jjxzkx+LcoFN2k Fig3B ii ------- COMMENT: b3bc9cbfdcacc24c 6 kWGPf21GWjHlwJTzda9fslGqHn4 Fig3Biii Cells shown a normal tea1 delta morphology ------- COMMENT: b3bc9cbfdcacc24c 7 InAoyMdP/QvHvIvvNV5kmSvzrUU Fig 4B ------- COMMENT: b3bc9cbfdcacc24c 8 InAoyMdP/QvHvIvvNV5kmSvzrUU Fig 4B ------- COMMENT: b3bc9cbfdcacc24c 9 EQnxyqnd3mWXYVWn7Ayc9pAaRN0 Fig4C, D Cells shown a normal tea1 delta actin morphology ------- COMMENT: b3bc9cbfdcacc24c 10 8Bfe9Cak+Lc8NE4KYnce9AOU+B8 Fig 4E ------- COMMENT: b3bc9cbfdcacc24c 12 BBWTJ95qbWj0/J4PJo0Mk8ZSYiI Fig4F Cells have a similar defect to a tea1 delta cell wall defect ------- COMMENT: b3bc9cbfdcacc24c 13 UGKLbMSoFrW9s5nNAMBghTw/TiA Fig 5B ------- COMMENT: b3bc9cbfdcacc24c 15 JKGccfibgqGyWP8ICHC7sQPQkyE Fig 5C, D ------- COMMENT: b3bc9cbfdcacc24c 16 CQGtsMsb1Fz3rI4URnEB3BTVeqI Data not shown ------- COMMENT: b3bc9cbfdcacc24c 17 B5kuU22A8v2CBb2zeFG0PcS7nzw Fig5D ------- COMMENT: b3bc9cbfdcacc24c 18 PuBwkNVO/ON8IDC3SZpV4hOQJBg Fig 6B, C ------- COMMENT: b3bc9cbfdcacc24c 19 M9juvCneX0OJZCB2t0ZBjwYHP+4 Fig6D ------- COMMENT: b3bc9cbfdcacc24c 20 tc3rJxZshAX+tmBq7BR9TkADO4o Fig 7A ------- COMMENT: b3bc9cbfdcacc24c 21 xwlCADUew2BxSTvwTxCObZXMyIk Fig 7B, C (comment: Penetrance refers to the penetrance of the NTR old-new end growth pattern) ------- COMMENT: b3bc9cbfdcacc24c 22 V7bce/xq7FxiW5sGjUhaBclPRes Fig8. (comment: CHECK pREP3X tea1 is a multi copy plasmid and is over expressed from the nmt1 promoter) ------- COMMENT: b3bc9cbfdcacc24c 23 abaxUCRt75zTda7whC5GUF/Fnxw Data not shown. (comment: CHECK pREP3X tea1 is a multi copy plasmid and is over expressed from the nmt1 promoter) ------- COMMENT: b3c499fa6bc66af4 58 AG7SbQXc4+DLVelJ4X0b47pOlsc (comment: higher than without nup132d) ------- COMMENT: b3c499fa6bc66af4 59 AG7SbQXc4+DLVelJ4X0b47pOlsc (comment: higher than without nup132d) ------- COMMENT: b3c499fa6bc66af4 66 9Dlv/HYKxu/k1mLimFwGI6o5Wok (comment: modification(s) not identified) ------- COMMENT: b3c499fa6bc66af4 69 9Dlv/HYKxu/k1mLimFwGI6o5Wok (comment: modification(s) not identified) ------- COMMENT: b401e8548347ee00 33 z69jGdEd26wb/iw7J9/T46QTd/c (comment: CHECK epistasis with Rhp51) ------- COMMENT: b401e8548347ee00 34 z69jGdEd26wb/iw7J9/T46QTd/c (comment: CHECK epistasis with Rhp51) ------- COMMENT: b40507851e928932 17 uf0AaaqiSTYkCBk53EIVIDmwBkA (comment: temperature permissive for single mutant without rad2delta) ------- COMMENT: b40507851e928932 18 uf0AaaqiSTYkCBk53EIVIDmwBkA (comment: temperature permissive for single mutant without rad2delta) ------- COMMENT: b40507851e928932 19 uf0AaaqiSTYkCBk53EIVIDmwBkA (comment: temperature permissive for single mutant without rad2delta) ------- COMMENT: b40507851e928932 20 uf0AaaqiSTYkCBk53EIVIDmwBkA (comment: temperature permissive for single mutant without rad2delta) ------- COMMENT: b40507851e928932 21 uf0AaaqiSTYkCBk53EIVIDmwBkA (comment: temperature permissive for single mutant without rad2delta) ------- COMMENT: b40507851e928932 22 uf0AaaqiSTYkCBk53EIVIDmwBkA (comment: temperature permissive for single mutant without rad2delta) ------- COMMENT: b40507851e928932 23 uf0AaaqiSTYkCBk53EIVIDmwBkA (comment: temperature permissive for single mutant without rad2delta) ------- COMMENT: b40507851e928932 24 t3fY9TjIK7cGkL49OiI0vBHHjWs (comment: temperature permissive for single mutant without cds1delta) ------- COMMENT: b40507851e928932 25 t3fY9TjIK7cGkL49OiI0vBHHjWs (comment: temperature permissive for single mutant without cds1delta) ------- COMMENT: b40507851e928932 26 t3fY9TjIK7cGkL49OiI0vBHHjWs (comment: temperature permissive for single mutant without cds1delta) ------- COMMENT: b40507851e928932 27 uf0AaaqiSTYkCBk53EIVIDmwBkA (comment: temperature permissive for single mutant without rad2delta) ------- COMMENT: b40507851e928932 28 t3fY9TjIK7cGkL49OiI0vBHHjWs (comment: temperature permissive for single mutant without cds1delta) ------- COMMENT: b40507851e928932 29 uf0AaaqiSTYkCBk53EIVIDmwBkA (comment: temperature permissive for single mutant without rad2delta) ------- COMMENT: b40507851e928932 31 uf0AaaqiSTYkCBk53EIVIDmwBkA (comment: temperature permissive for single mutant without rad2delta) ------- COMMENT: b40507851e928932 32 t3fY9TjIK7cGkL49OiI0vBHHjWs (comment: temperature permissive for single mutant without cds1delta) ------- COMMENT: b40507851e928932 33 t3fY9TjIK7cGkL49OiI0vBHHjWs (comment: temperature permissive for single mutant without cds1delta) ------- COMMENT: b40507851e928932 34 t3fY9TjIK7cGkL49OiI0vBHHjWs (comment: temperature permissive for single mutant without cds1delta) ------- COMMENT: b40507851e928932 35 t3fY9TjIK7cGkL49OiI0vBHHjWs (comment: temperature permissive for single mutant without cds1delta) ------- COMMENT: b40507851e928932 36 t3fY9TjIK7cGkL49OiI0vBHHjWs (comment: temperature permissive for single mutant without cds1delta) ------- COMMENT: b40507851e928932 37 t3fY9TjIK7cGkL49OiI0vBHHjWs (comment: temperature permissive for single mutant without cds1delta) ------- COMMENT: b40507851e928932 38 t3fY9TjIK7cGkL49OiI0vBHHjWs (comment: temperature permissive for single mutant without cds1delta) ------- COMMENT: b40507851e928932 39 t3fY9TjIK7cGkL49OiI0vBHHjWs (comment: temperature permissive for single mutant without cds1delta) ------- COMMENT: b40507851e928932 42 GM+gSGblxcgPz79mThj/wlyORoc (comment: temperature permissive for single mutant without rad26delta) ------- COMMENT: b40507851e928932 43 GM+gSGblxcgPz79mThj/wlyORoc (comment: temperature permissive for single mutant without rad26delta) ------- COMMENT: b40507851e928932 44 GM+gSGblxcgPz79mThj/wlyORoc (comment: temperature permissive for single mutant without rad26delta) ------- COMMENT: b40507851e928932 45 GM+gSGblxcgPz79mThj/wlyORoc (comment: temperature permissive for single mutant without rad26delta) ------- COMMENT: b40507851e928932 46 GM+gSGblxcgPz79mThj/wlyORoc (comment: temperature permissive for single mutant without rad26delta) ------- COMMENT: b40507851e928932 47 GM+gSGblxcgPz79mThj/wlyORoc (comment: temperature permissive for single mutant without rad26delta) ------- COMMENT: b40507851e928932 48 GM+gSGblxcgPz79mThj/wlyORoc (comment: temperature permissive for single mutant without rad26delta) ------- COMMENT: b40507851e928932 49 GM+gSGblxcgPz79mThj/wlyORoc (comment: temperature permissive for single mutant without rad26delta) ------- COMMENT: b40507851e928932 50 GM+gSGblxcgPz79mThj/wlyORoc (comment: temperature permissive for single mutant without rad26delta) ------- COMMENT: b40507851e928932 51 GM+gSGblxcgPz79mThj/wlyORoc (comment: temperature permissive for single mutant without rad26delta) ------- COMMENT: b419f152633d75f3 1 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: b419f152633d75f3 2 y8ECh8MAm66uW9NV0ySZuEY3bVo (comment: CHECK VW REQUESTED ABOLISHED) Fig. 4E Both H3K9me2 and H3K9me3 were completely abolished in clr4-GS253 and clr4-3FA strains at centromeric dg/dh repeats, being indistinguishable from clr4Δ ------- COMMENT: b419f152633d75f3 3 wu20zGpQDCqCwdS5l6NYIcOFH/Y Fig. 4D (comment: Currently, we cannot explain why the dg transcripts in the 3FA mutant are only slightly elevated while completely losing H3K9me2/3.) ------- COMMENT: b419f152633d75f3 4 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: b419f152633d75f3 5 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: b419f152633d75f3 6 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: b419f152633d75f3 7 SyPjM0SeIskqqIcpu8IzGdiP3js Fig. 4E (comment: CHECK REQUESTED ABOLISHED) ------- COMMENT: b419f152633d75f3 8 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: b419f152633d75f3 9 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: b419f152633d75f3 10 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: b419f152633d75f3 11 vkT8uMpiBxgGlmYXEA8V3+LXigM Fig. 4E ------- COMMENT: b419f152633d75f3 12 vkT8uMpiBxgGlmYXEA8V3+LXigM Fig. 4E ------- COMMENT: b419f152633d75f3 13 vkT8uMpiBxgGlmYXEA8V3+LXigM Fig. 4E ------- COMMENT: b419f152633d75f3 14 vkT8uMpiBxgGlmYXEA8V3+LXigM Fig. 4E ------- COMMENT: b419f152633d75f3 15 iGSQJJ1u4yXnDGWp/3IrsqAQ+tA hht-ub14 substrate which both manifest a similar degree of strong stimulation by H3K14ub (Figure 1C, Figure 1—figure supplement 1E–H). ------- COMMENT: b419f152633d75f3 18 YoazKYt6C+n2wpyXwqYEAMjREMo Figure 3F, fig1 These experiments confirm the observation by Oya et al., 2019 that the H3K14ub substrate triggers a dramatic and specific increase in the methyltransferase activity of Clr4. However, in contrast to the previous study, we observe that the KMT domain is sufficient to mediate this regulatory mechanism. ------- COMMENT: b419f152633d75f3 19 H/Qcd75QBezWR/kDu8O1xRriukI Figure 3F, fig1 ------- COMMENT: b419f152633d75f3 20 H/Qcd75QBezWR/kDu8O1xRriukI Figure 3F, fig1 ------- COMMENT: b419f152633d75f3 22 y7WA2jufsHAT+hE594qc5IgRr0Q (comment: consistent with Clr4’s KMT domain mediating the crosstalk between H3K14ub and H3K9me2/3 as an essential step in heterochromatin formation and maintenance.) ------- COMMENT: b42a7324f08b6a24 8 fjuCQqbgk2nDyrc5Vc3liIYljQQ (comment: CHECK during(GO:0051329)) ------- COMMENT: b42a7324f08b6a24 13 2BS5cwiaoJNCstrOO/bvGlxEPzc (comment: dependent on septation initiation signaling (GO:0031028)) ------- COMMENT: b42cb21b34629e92 6 TywczxxPzK/0pOjofj87YQhav0s (comment: CHECK activated_by(CHEBI:18420)| activated_by(CHEBI:29103)| inhibited_by(CHEBI:48607)| inhibited_by(CHEBI:26710)) ------- COMMENT: b449ba7fcde22f63 6 J0aqpbBvwP2S79icCGoEZimYMvY fig 4a ------- COMMENT: b449ba7fcde22f63 7 J0aqpbBvwP2S79icCGoEZimYMvY fig 4a ------- COMMENT: b449ba7fcde22f63 8 J0aqpbBvwP2S79icCGoEZimYMvY fig 4a ------- COMMENT: b449ba7fcde22f63 9 J0aqpbBvwP2S79icCGoEZimYMvY fig 4a ------- COMMENT: b449ba7fcde22f63 10 J0aqpbBvwP2S79icCGoEZimYMvY fig 4a ------- COMMENT: b45eb4ced4f20d69 1 G4UJkpsur2+ya17QfekCpuD6lf8 Expression of dap1+ mRNA was induced in the absence of oxygen in a Sre1-de- pendent manner (Figure 1A), ------- COMMENT: b45eb4ced4f20d69 2 cBg+CbijXCcd02nOTkScZqqIxyE dap1D cells were viable under normal growth con- ditions but ..... ------- COMMENT: b45eb4ced4f20d69 3 tDa+TCtsug9qJDzR4z0a4LvcLVQ ...contained a reduced amount of ergosterol and elevated amounts of the ergosterol biosynthetic inter- mediates 24-methylene lanosterol, ergosta-5,7,24(28)-tri- enol, and ergosta-5,7-dienol, consistent with defects at the Erg11 and Erg5 enzymatic steps (Figure 1B). ------- COMMENT: b45eb4ced4f20d69 4 1E+9o4/0be1epAoi+tfHAdp8rrA elevated amounts of the ergosterol biosynthetic inter- mediates 2. upon loss of Dap1, cells accumulated 24-methylene lanosterol, ergosta- 5,7,24(28)-trienol, and ergosta-5,7-dienol, substrates for Erg11 and Erg5. 4-methylene lanosterol, ergosta-5,7,24(28)-tri- enol, and ergosta-5,7-dienol, Figure 1B. Ergo- sta-5,7-dienol is not a normal pathway intermediate, but forms when Erg5 is inhibited (Figure S1) ------- COMMENT: b45eb4ced4f20d69 5 bmvc7TgD1TDOtYb67z+awh5c9PA sensitive to the inhibitors of sterol syn- thesis itraconazole and CoCl2 (Figure 1D). ------- COMMENT: b45eb4ced4f20d69 6 gxb/vqQUqpoSDa8eIN9MafM0Rrw These data demonstrate that Dap1 is required in vivo for the activity of Erg11 and Erg5, the entire complement of cytochrome P450 enzymes in fission yeast. ------- COMMENT: b45eb4ced4f20d69 7 gxb/vqQUqpoSDa8eIN9MafM0Rrw These data demonstrate that Dap1 is required in vivo for the activity of Erg11 and Erg5, the entire complement of cytochrome P450 enzymes in fission yeast. ------- COMMENT: b45eb4ced4f20d69 8 gxb/vqQUqpoSDa8eIN9MafM0Rrw These data demonstrate that Dap1 is required in vivo for the activity of Erg11 and Erg5, the entire complement of cytochrome P450 enzymes in fission yeast. ------- COMMENT: b45eb4ced4f20d69 9 KvTnPCHzsQpqXiHHcs5tHGYx9Mk However, similar to dap1D, yeast carrying the HA-dap1 Y138F plasmid accumulated 24-methylene lanosterol, ergosta-5,7,24(28)-trienol, and ergosta-5,7- dienol, reflecting defects in Erg11 and Erg5 (Figure 3B). These data indicate that dap1 Y138F is a loss-of-function mutation and that Dap1 function requires bound heme. ------- COMMENT: b45eb4ced4f20d69 10 1E+9o4/0be1epAoi+tfHAdp8rrA elevated amounts of the ergosterol biosynthetic inter- mediates 2. upon loss of Dap1, cells accumulated 24-methylene lanosterol, ergosta- 5,7,24(28)-trienol, and ergosta-5,7-dienol, substrates for Erg11 and Erg5. 4-methylene lanosterol, ergosta-5,7,24(28)-tri- enol, and ergosta-5,7-dienol, Figure 1B. Ergo- sta-5,7-dienol is not a normal pathway intermediate, but forms when Erg5 is inhibited (Figure S1) ------- COMMENT: b45eb4ced4f20d69 11 1E+9o4/0be1epAoi+tfHAdp8rrA elevated amounts of the ergosterol biosynthetic inter- mediates 2. upon loss of Dap1, cells accumulated 24-methylene lanosterol, ergosta- 5,7,24(28)-trienol, and ergosta-5,7-dienol, substrates for Erg11 and Erg5. 4-methylene lanosterol, ergosta-5,7,24(28)-tri- enol, and ergosta-5,7-dienol, Figure 1B. Ergo- sta-5,7-dienol is not a normal pathway intermediate, but forms when Erg5 is inhibited (Figure S1) ------- COMMENT: b466cec3cdc7c5c0 1 N/J9gJhOiLTwb7SmpD5Hae7jTHc An increased resistance to cell lysis induced by the presence of higher concentration of 2-deoxyglucose in the vegetative growth phase of life cycle of cell ------- COMMENT: b466cec3cdc7c5c0 2 6aXYyoDU6zU9gjhoHan6MAkp6QI An increased resistance to cell lysis induced by the presence of higher concentration of 2-deoxyglucose in the vegetative growth phase of cell ------- COMMENT: b466cec3cdc7c5c0 6 6aXYyoDU6zU9gjhoHan6MAkp6QI An increased resistance to cell lysis induced by the presence of higher concentration of 2-deoxyglucose in the vegetative growth phase of cell ------- COMMENT: b466cec3cdc7c5c0 7 6aXYyoDU6zU9gjhoHan6MAkp6QI An increased resistance to cell lysis induced by the presence of higher concentration of 2-deoxyglucose in the vegetative growth phase of cell ------- COMMENT: b466cec3cdc7c5c0 8 6aXYyoDU6zU9gjhoHan6MAkp6QI An increased resistance to cell lysis induced by the presence of higher concentration of 2-deoxyglucose in the vegetative growth phase of cell ------- COMMENT: b466cec3cdc7c5c0 9 bpHdoNa2V8Np4gIdI5hqijJG6Ig A phenotype in which a cell lyses, i.e. the plasma membrane ruptures and cytoplasm is lost, in presence of higher concentration of 2-deoxyglucose. ------- COMMENT: b5450581ac9e067c 3 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: b5450581ac9e067c 4 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: b5450581ac9e067c 5 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: b5450581ac9e067c 6 TUl9fngQi9T2r1Ci1WAnQSqMOPg fig 1 a ------- COMMENT: b5450581ac9e067c 15 qolWwpj100oJGsncz+ur9zL7hP0 fig1 c ------- COMMENT: b5450581ac9e067c 16 qolWwpj100oJGsncz+ur9zL7hP0 fig1 c ------- COMMENT: b5450581ac9e067c 17 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: b5450581ac9e067c 18 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: b5450581ac9e067c 30 Hy80rlav4jqSUKmEtWGY8FOaEOY (comment: CHECK TER1 level only but not telomere length) ------- COMMENT: b5450581ac9e067c 31 VwDiogZVodjIfs+85PFEhkkvU1o fig 3a ------- COMMENT: b5617d852b7dbffb 21 1ysuEsSrAeJAhpjlKDAKm/9FD/8 (comment: temperature semi-permissive for cdc8-27) ------- COMMENT: b5617d852b7dbffb 22 1ysuEsSrAeJAhpjlKDAKm/9FD/8 (comment: temperature semi-permissive for cdc8-27) ------- COMMENT: b5617d852b7dbffb 27 1ysuEsSrAeJAhpjlKDAKm/9FD/8 (comment: temperature semi-permissive for cdc8-27) ------- COMMENT: b5617d852b7dbffb 31 1ysuEsSrAeJAhpjlKDAKm/9FD/8 (comment: temperature semi-permissive for cdc8-27) ------- COMMENT: b595ffbb38089b55 1 qtxgL+U03MzeNREQMQIXylaLk1Q (comment: from PM ) (Fig. 3c ------- COMMENT: b595ffbb38089b55 5 PEijj9MIrNSBSecC4MlXqO1v4Zk (Fig. 3) ------- COMMENT: b595ffbb38089b55 6 PEijj9MIrNSBSecC4MlXqO1v4Zk (Fig. 3) ------- COMMENT: b5b2fbc60c16e523 1 nCpUAEjr+wpvt/5IjPxvd42pAEw Tetrad analysis showed that all four haploid spores of the heterozygous diploid produced colonies at 28°C, indicating that the wis2+ gene is non- essential for viability under normal growth conditions. ------- COMMENT: b5b2fbc60c16e523 2 AX/FJDZ2snZxo8VlJKh1HJjKyz8 Microscopic examination failed to detect any difference in cell size or morphology between wis2+-deleted cels (wis2A) and wild-type cels,over a range oftemperatures from 20 to 36°C. ------- COMMENT: b5b9e2e7b7d1213e 7 Ah9YUbjOEpI3moc6CZlS8TLCDZI (comment: one or more of mutated serine residues) ------- COMMENT: b5b9e2e7b7d1213e 8 Ah9YUbjOEpI3moc6CZlS8TLCDZI (comment: one or more of mutated serine residues) ------- COMMENT: b5db93caaa51792d 2 WENrqn3XpW5Z2joPis9/yxuQExw (comment: CHECK transferres from term suggestion to allow approval decreased growth under low-glucose conditions Definition: "decreased growth under low-glucose conditions" is an "decreased cell population growth" that occurs specifically on media supplied with a lower concentration of glucose than regular media. ditto all the others) ------- COMMENT: b5e73159bb3ebdf8 9 Ginn8SUbUSEXvmw8cV1DM9oKO5A cells are longer that wt under N starvation conditions, but not necessarily longer than a wt cell is when not starved for N ------- COMMENT: b5e73159bb3ebdf8 10 Ginn8SUbUSEXvmw8cV1DM9oKO5A cells are longer that wt under N starvation conditions, but not necessarily longer than a wt cell is when not starved for N ------- COMMENT: b5e73159bb3ebdf8 11 Ginn8SUbUSEXvmw8cV1DM9oKO5A cells are longer that wt under N starvation conditions, but not necessarily longer than a wt cell is when not starved for N ------- COMMENT: b5e73159bb3ebdf8 12 Ginn8SUbUSEXvmw8cV1DM9oKO5A cells are longer that wt under N starvation conditions, but not necessarily longer than a wt cell is when not starved for N ------- COMMENT: b5e73159bb3ebdf8 13 Ginn8SUbUSEXvmw8cV1DM9oKO5A cells are longer that wt under N starvation conditions, but not necessarily longer than a wt cell is when not starved for N ------- COMMENT: b5e73159bb3ebdf8 31 Ginn8SUbUSEXvmw8cV1DM9oKO5A cells are longer that wt under N starvation conditions, but not necessarily longer than a wt cell is when not starved for N ------- COMMENT: b5e73159bb3ebdf8 32 Ginn8SUbUSEXvmw8cV1DM9oKO5A cells are longer that wt under N starvation conditions, but not necessarily longer than a wt cell is when not starved for N ------- COMMENT: b5e73159bb3ebdf8 33 Ginn8SUbUSEXvmw8cV1DM9oKO5A cells are longer that wt under N starvation conditions, but not necessarily longer than a wt cell is when not starved for N ------- COMMENT: b5faa30667b9677e 3 SxdKEJ9PFYtzqKwSbQwQ8l484k0 (comment: Biochemistry) ------- COMMENT: b5faa30667b9677e 4 napGEt6quFQMrpmFS0Q4uG8yOK8 (comment: MT spindown assay.) ------- COMMENT: b5faa30667b9677e 5 t+RJvut1MyAtf5Ko0dtlFYPBiZA (comment: MT spindown assay) ------- COMMENT: b5faa30667b9677e 13 1ha5CSkDt+DHvxzi9cUzeE214Iw (comment: CHECK partially rescues FYPO:0002818 and FYPO:0005558) ------- COMMENT: b5fde7a48c34bee4 46 G1UbtpeYSsTZrZS/AhfrTV8fC1s Figure 5F ------- COMMENT: b5ff1c5ed18d1f76 1 5D8clNVuOX/E1G6sCYn6SKYEurc GFP-Rdp1 was not detected in the nuclei of most cells (Figure 1B). ------- COMMENT: b5ff1c5ed18d1f76 2 5D8clNVuOX/E1G6sCYn6SKYEurc GFP-Rdp1 was not detected in the nuclei of most cells (Figure 1B). ------- COMMENT: b5ff1c5ed18d1f76 3 Uv1Lx9P1az69KkPs6iC1oLWZ7gY localization of Dcr1-GFP and GFP-Ago1 was not affected by the loss of Sal3 activity (Figure S1). ------- COMMENT: b5ff1c5ed18d1f76 4 Wo/Jh3uo6kfOxUVzFZ5IDV4Qv2o localization of Dcr1-GFP and GFP-Ago1 was not affected by the loss of Sal3 activity (Figure S1). ------- COMMENT: b5ff1c5ed18d1f76 5 T8mjm56Z/x7MajjasCp9JC4WBBM onsistent with the scenario in which Rdp1 is a cargo for Sal3, we were able to detect stable interaction between tagged Rdp1 and Sal3 by co-immunoprecipitation (Figure 2B). ------- COMMENT: b5ff1c5ed18d1f76 6 vuB2pj0fbF/hxcCo/SUlap2WFZE Pericentric transcript levels are increased in sal3 mutants ------- COMMENT: b5ff1c5ed18d1f76 7 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: b5ff1c5ed18d1f76 8 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: b5ff1c5ed18d1f76 10 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: b5ff1c5ed18d1f76 11 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: b5ff1c5ed18d1f76 12 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: b5ff1c5ed18d1f76 13 y3keauJQYwDWKdRfjwSFVG4dK5A Moreover, expression of the Rdp1-SV40-NLS construct appeared to suppress centromeric transcript levels below that those found in wild type ------- COMMENT: b5ff1c5ed18d1f76 14 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: b6055c42bedc6e07 1 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: b6055c42bedc6e07 2 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: b6055c42bedc6e07 3 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: b6055c42bedc6e07 4 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: b6055c42bedc6e07 5 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: b6055c42bedc6e07 6 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: b6055c42bedc6e07 7 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: b6055c42bedc6e07 8 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: b6055c42bedc6e07 9 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: b6055c42bedc6e07 10 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: b6055c42bedc6e07 11 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: b6055c42bedc6e07 12 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: b6055c42bedc6e07 13 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: b6055c42bedc6e07 14 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: b6055c42bedc6e07 15 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: b6055c42bedc6e07 16 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: b6055c42bedc6e07 17 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: b6055c42bedc6e07 18 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: b6055c42bedc6e07 19 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: b6055c42bedc6e07 20 3dh6vdthNGt/28xFkGhxPSPSSBs Fig. 3B and 4B ------- COMMENT: b6055c42bedc6e07 21 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 22 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 23 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 24 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 25 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 26 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 27 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 28 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 29 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 30 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 31 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 32 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 33 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 34 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 35 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 36 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 37 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 38 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 39 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 40 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 41 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 42 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 43 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 44 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 45 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 46 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 47 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 48 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 49 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 50 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: b6055c42bedc6e07 51 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: b6055c42bedc6e07 52 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: b6055c42bedc6e07 53 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: b6055c42bedc6e07 54 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: b6055c42bedc6e07 55 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: b6055c42bedc6e07 56 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: b6055c42bedc6e07 57 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: b6055c42bedc6e07 58 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: b6055c42bedc6e07 59 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: b6055c42bedc6e07 60 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: b6055c42bedc6e07 61 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: b6055c42bedc6e07 62 dJ55jf9F7w1w+3+W5RpemgHi8WI Fig. 5B and C ------- COMMENT: b6055c42bedc6e07 63 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: b6055c42bedc6e07 64 dJ55jf9F7w1w+3+W5RpemgHi8WI Fig. 5B and C ------- COMMENT: b6055c42bedc6e07 65 dJ55jf9F7w1w+3+W5RpemgHi8WI Fig. 5B and C ------- COMMENT: b6055c42bedc6e07 66 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: b6055c42bedc6e07 67 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: b6055c42bedc6e07 68 dJ55jf9F7w1w+3+W5RpemgHi8WI Fig. 5B and C ------- COMMENT: b6055c42bedc6e07 69 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: b6055c42bedc6e07 70 EobMH5Fs9kajUdmQXd6WEl2Ly/0 Set3 is targeted to the promoters of clr4+ and rik1+, probably through its PHD finger. Set3 promotes transcription of clr4+ and rik1+. ------- COMMENT: b68179e7a7d13aff 30 PxGYNeSxrV5PD+XotSQQNBxfnSo fig 1a ------- COMMENT: b68179e7a7d13aff 31 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: b68179e7a7d13aff 32 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: b68179e7a7d13aff 33 PxGYNeSxrV5PD+XotSQQNBxfnSo fig 1a ------- COMMENT: b687ff7ab68d1820 1 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: b687ff7ab68d1820 3 Z6tEIFMvHePTiPE8ZKnvdnLWmOI Figure 1C ------- COMMENT: b687ff7ab68d1820 5 CdEXpM80T5OrSiO7etYm9wGSc/g figure 3 ------- COMMENT: b687ff7ab68d1820 7 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: b6b93831c9879682 56 W6ABl9xDpkeHDhSQSa00SyZKzZw (comment: same as cdc2-33 alone) ------- COMMENT: b6b93831c9879682 59 YxJ8oJF6kcPzaXe4NMuJV2iMI/4 (comment: same as orb3-167 alone) ------- COMMENT: b6b93831c9879682 60 YxJ8oJF6kcPzaXe4NMuJV2iMI/4 (comment: same as orb3-167 alone) ------- COMMENT: b6b93831c9879682 61 YxJ8oJF6kcPzaXe4NMuJV2iMI/4 (comment: same as orb3-167 alone) ------- COMMENT: b6b93831c9879682 62 Z9w8YyMnbA2Mm3m7O8akuD4js2w (comment: same as orb2-34 alone) ------- COMMENT: b6b93831c9879682 63 Z9w8YyMnbA2Mm3m7O8akuD4js2w (comment: same as orb2-34 alone) ------- COMMENT: b6b9d534da028509 10 UUIGAGYIlzNh3/CZeXrmr/tr93Q RTS1 inversion background abolishes DSB formation; "decreased" level in rtf1-W405G is relative to wild type and above the inverted-RTS1 background level ------- COMMENT: b6c50bbff2ebe7ce 1 7mkLHEnYLeBNaFfLUHlWAiGK6aU Collectively, these results indicate that in fission yeast, a ?-TuRC- like complex exists as a stable structure in vivo independent of the Mto1/2 complex ------- COMMENT: b6f52423002339f8 1 RWSH4JIdUCWCHDslUr8oDL7UtR0 (comment: Microscopy) ------- COMMENT: b6f52423002339f8 3 QGV1MG7chD/wY166PwZFFxDPQJU Fig 1a-c ------- COMMENT: b6f52423002339f8 4 QGV1MG7chD/wY166PwZFFxDPQJU Fig 1a-c ------- COMMENT: b6f52423002339f8 5 3IIZKd5m6W0pGR2ur3U7rMT0A9A fig 1d ------- COMMENT: b6f52423002339f8 6 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: b6f52423002339f8 7 SyaI/HpAnhvtV4eDXhRf23kjMgc (comment: Dot-Blot assay) ------- COMMENT: b6f52423002339f8 8 WgSKNHV1HJKDzBoUq+u6lvqy40s Evaluated by measuring the size of Vps10-GFP foci ------- COMMENT: b6f52423002339f8 9 CRFW7KDT9+ZTVDTvqxJkBmTliiE (comment: CONDITION 37ºC) ------- COMMENT: b6f52423002339f8 10 kti5qhXFB2yZMgNe4CY+OdwMeqQ (comment: CONDITION 28ºC) ------- COMMENT: b6f52423002339f8 12 VvffgU7cVYm5g0Xh7QeaqPOQJBk fig 1 Increased colocalization with Cfr1 ------- COMMENT: b6f52423002339f8 13 lpMkkHLBxATg2714lGLUCRMjlAQ Reduced co-localyzation with the PI3P probe Cherry-FYVE ------- COMMENT: b6f52423002339f8 14 pZERl4XmGWTN1nv4ROWByoCqDdo Reduced Vps10 exit from the Golgi ------- COMMENT: b6f52423002339f8 15 qQquvpZ595fKt67LmwLz0Vj+XXc Abnormal Vps10 proteolytic degradation ------- COMMENT: b6f52423002339f8 16 nRy16g6VnA6t0/oNYDVeCvhXp08 Abnormal Cps1 transport and processing ------- COMMENT: b6f52423002339f8 17 wjKUW1CGjkmwDPq5iQw/3WbYK/o Abnormal Syb1 recycling ------- COMMENT: b6f52423002339f8 18 tNYc1Q/6vU2pgk7WKb1xuiGEXOU Abnormal endosome organization ------- COMMENT: b6f52423002339f8 19 luYNAJcxEVtlo4yiUFGUyadf/mw Reduced growth on KCl plates ------- COMMENT: b6f52423002339f8 20 pZERl4XmGWTN1nv4ROWByoCqDdo Reduced Vps10 exit from the Golgi ------- COMMENT: b6f52423002339f8 21 qQquvpZ595fKt67LmwLz0Vj+XXc Abnormal Vps10 proteolytic degradation ------- COMMENT: b6f52423002339f8 22 nRy16g6VnA6t0/oNYDVeCvhXp08 Abnormal Cps1 transport and processing ------- COMMENT: b6f52423002339f8 23 wjKUW1CGjkmwDPq5iQw/3WbYK/o Abnormal Syb1 recycling ------- COMMENT: b6f52423002339f8 24 tNYc1Q/6vU2pgk7WKb1xuiGEXOU Abnormal endosome organization ------- COMMENT: b6f52423002339f8 25 w93p/as5N+YWfN8JIZ4Kbt5Gqus (comment CHECK Cpy1 secretion) ------- COMMENT: b6f52423002339f8 26 T0x8OWihYFb+ut/JQX/NFTTosSw Co-localization with TGN marker ------- COMMENT: b6f52423002339f8 27 YfJ8WQrH7X9iyk17YIZGSnKBhLI FIg 3 Increased co-localization with Cfr1 ------- COMMENT: b6f52423002339f8 28 DlHC8ov5E7zl+eFY5sYLxQnfk8k Reduced co-localization with the PI3P probe Cherry-FYVE ------- COMMENT: b6f52423002339f8 30 ETq9buw/Bwx74Tgahc+fjWgSjL4 Reduced growth at 37ºC on YES plates ------- COMMENT: b6f52423002339f8 31 qQquvpZ595fKt67LmwLz0Vj+XXc Abnormal Vps10 proteolytic degradation ------- COMMENT: b6f52423002339f8 32 qQquvpZ595fKt67LmwLz0Vj+XXc Abnormal Vps10 proteolytic degradation ------- COMMENT: b6f52423002339f8 34 ni3gGcaahMyX+gXz9abVqj8xKs0 Increased co-localization with Cfr1 ------- COMMENT: b6f52423002339f8 35 DlHC8ov5E7zl+eFY5sYLxQnfk8k Reduced co-localization with the PI3P probe Cherry-FYVE ------- COMMENT: b6f52423002339f8 36 ni3gGcaahMyX+gXz9abVqj8xKs0 Increased co-localization with Cfr1 ------- COMMENT: b6f52423002339f8 39 QraF2LoYi7rihNqt5zs1+hv7Fdg (comment: Dot-Blot test) ------- COMMENT: b6f52423002339f8 40 7ap5G50rV48lnHWgfnlr9HVsMOU fig 7 Syb1 co-localizes with late endosome markers ------- COMMENT: b6f52423002339f8 41 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: b6f52423002339f8 42 XLklol0gP/0SMvtIijCfNsb9YsA Reduced growth at 37ºC on YES agar plates ------- COMMENT: b6f52423002339f8 43 doK8LFxMmEw9x7MbsbkDMhIG2YY Reduced growth on 0.6M KCl plates ------- COMMENT: b6f52423002339f8 45 DMHCjgR/34n2vMwKdds5jvEqH8Y isp6 delta supresses the abnormal Vps10 proessing detected in gga21 delta gga22 delta strain ------- COMMENT: b6f52423002339f8 46 yoWa8dmJlUJiIa0VyO3xEqk/ENs isp6 delta suppresses Vps10 abnormal processing observed in ent3 delta gga22 delta strain ------- COMMENT: b6f52423002339f8 47 7ThtT3rILUwV3u5toKe02IuyvZA isp6 delta supresses the abnormal Vps10 processing detected in ent3 delta gga21 delta gga22 delta strain ------- COMMENT: b6f52423002339f8 48 RWSH4JIdUCWCHDslUr8oDL7UtR0 (comment: Microscopy) ------- COMMENT: b6f52423002339f8 49 RWSH4JIdUCWCHDslUr8oDL7UtR0 (comment: Microscopy) ------- COMMENT: b6f52423002339f8 50 n3qFPUVPnygXj7DYTh7tfFk3zQo isp6 delta supresses the abnormal Vps10 processing detected in vps35 delta strain ------- COMMENT: b6f52423002339f8 55 H/hIjIWVnHCZsgI+BmfXppn7TIw Fig1 (comment: minor) ------- COMMENT: b6f52423002339f8 56 JgZ4LGEcgK17dWQIgG5WWu9j/Uk Fig1 (comment: major) ------- COMMENT: b6f52423002339f8 57 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: b6f52423002339f8 58 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: b6f52423002339f8 60 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: b6f52423002339f8 62 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: b6f52423002339f8 63 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: b6f52423002339f8 64 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: b6f52423002339f8 68 Sm2hxno7PAYIyHCUa1s8SrjLI1s (comment: this is in a mutant but I guess it occurs physiologicall?) ------- COMMENT: b732a3ac22de7b6e 12 TZiWw2k4vxtZs8cxcKufSb3K8G8 (comment: CHECK phosphorylation of mei2 targets it for degradation via the proteasome) ------- COMMENT: b732a3ac22de7b6e 31 x/Wg8vszdh/2lnhsmWJB/MvDT6Y tor2 phosphorylates mei2. Phosphorylated mei2 is ubiquitylated which targets it for degradation via the proteasome. ------- COMMENT: b74b5e273e9ba8f0 1 NlTtAbqSqpgzr8+5ajiMQkp63/0 (comment: functionally complements S cerevisiae ABC1) ------- COMMENT: b792e3d40839cdbd 1 5vP3nJs6cQF9TmClP3dG3Jb4NIE Fig. 3B, Fig. 4A,B ------- COMMENT: b792e3d40839cdbd 2 E4sm2Z465ncVFt1m5mPXm/IyKlo Fig. 3C, D; Fig. 4A,B ------- COMMENT: b792e3d40839cdbd 3 C5DT7h4YvhaLVP2PQ2NxOMkC+IA Fig. 4A,B ------- COMMENT: b792e3d40839cdbd 4 pYPf3NE1j52RI95E8vhoEZR3VSc Fig. 4C. Resistant to 1mM spermidine at 37C. ------- COMMENT: b792e3d40839cdbd 5 GA1hfmLWARMbFRjcslcLnOjgLd8 Fig. 4C Resistant to 1 mM spermidine at 37C. ------- COMMENT: b793085c4da3e210 1 H/ebRtsvIqM9uWwBlzEF7BRoesY (comment: CHECK cdc25-dD,arf6delta) ------- COMMENT: b793085c4da3e210 2 cxsttiDXB+NwyQgembrjnNJ2r6s (comment: CHECK cdc25-22,syt22delta) ------- COMMENT: b793085c4da3e210 3 NQn1tc6c9BC555tQtMEvGHdkpdM pom1delta Septation Defect. All three double mutants exhibited synthetic defects in cell growth and cytokinesis as judged by tilted and disorganized septa (Fig. 5, C–E; and Fig. S3 M) ------- COMMENT: b793085c4da3e210 4 tcPyUks/5RR8lMRHsY4b6QPNe+w cdc4-31 Septation Defect ------- COMMENT: b793085c4da3e210 5 FbeUoO7Dl7EiH1kXJPjdTNyLT6M rng2-D5 Septation Defect ------- COMMENT: b793085c4da3e210 6 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: b793085c4da3e210 7 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: b793085c4da3e210 11 XYzZnx3w19KFXGEMjw9XeT1ovPY Fig S1A; (comment: 27 micron) ------- COMMENT: b793085c4da3e210 12 +IPBfmt9bqm+srat9tITeTvEJzg Fig S1A; (comment: 26.6 micron) ------- COMMENT: b793085c4da3e210 13 Iv7LscbPkzCWYInSCefFAIgOUNA Fig S1A; (comment: 24.6 micron) ------- COMMENT: b793085c4da3e210 14 hG84eS6eglt4b7yJMi01tHQRwsg This is from GIs, phenocopy, PLUS If the Arf6 localization defect in syt22Δ is due to loss of the GTP-bound state, then it should be suppressed by arf6(Q75L). Indeed, arf6(Q75L)-mNG localized to nodes even in syt22Δ cells (Fig. 2 G) ------- COMMENT: b793085c4da3e210 15 biSkKK9FRuBE7oq4FQWm3AmykM8 The cell length at division phenotype for arf6Δ was minor, but these cells were wider than wild type (Fig. S1 E). ------- COMMENT: b793085c4da3e210 16 V2Fs4BVM1xgB4ZGJhV9IWFXJrYc The slower-migrating, hyperphosphorylated form of Wee1 was lost in arf6Δ and syt22Δ, similar to cdr2Δ (Fig. 1 H). We conclude that activated Arf6 functions in the Cdr2 pathway to control cell size at division through inhibition of Wee1. ------- COMMENT: b793085c4da3e210 17 V2Fs4BVM1xgB4ZGJhV9IWFXJrYc The slower-migrating, hyperphosphorylated form of Wee1 was lost in arf6Δ and syt22Δ, similar to cdr2Δ (Fig. 1 H). We conclude that activated Arf6 functions in the Cdr2 pathway to control cell size at division through inhibition of Wee1. ------- COMMENT: b793085c4da3e210 18 V2Fs4BVM1xgB4ZGJhV9IWFXJrYc The slower-migrating, hyperphosphorylated form of Wee1 was lost in arf6Δ and syt22Δ, similar to cdr2Δ (Fig. 1 H). We conclude that activated Arf6 functions in the Cdr2 pathway to control cell size at division through inhibition of Wee1. ------- COMMENT: b793085c4da3e210 19 tQGm145GEqiiWH3PKAjKR4Q4OdI Arf6 localizes stably to Cdr2 nodes during interphase in a manner that depends on nucleotide binding, membrane binding, and Cdr2 itself.; strongly enriched at cortical nodes in the cell middle (Fig. 2 A). Arf6 and Cdr2 colocalized at nodes (Figs. 2 B and S2 A) ------- COMMENT: b793085c4da3e210 20 G/zLHA+Ea3pNMvsbh4IFwOkSRVs trongly enriched at cortical nodes in the cell middle (Fig. 2 A). Arf6 and Cdr2 colocalized at nodes (Figs. 2 B and S2 A) ------- COMMENT: b793085c4da3e210 21 JryXhgsjdFPGAxNs25uA1JKNGfA Arf6 node localization required Cdr2 but not other node proteins (Figs. 2 E and S2 B). ------- COMMENT: b793085c4da3e210 22 ehyRj2vKyLQu3em5Jv3b2xhWWeo A GDP-locked mutant arf6(T52N)-mNG lost node localization, (Fig. 2 F) ------- COMMENT: b793085c4da3e210 23 NynNjX8X9iq80Lc5A3/fQHZsa7o but the GTP-locked allele arf6(Q75L)-mNG remained at nodes (Fig. 2 F) ------- COMMENT: b793085c4da3e210 24 8OYWynZBsRD4lRNLSGVA5nE36lI Further, Arf6 localization to nodes was lost upon deletion of its GEF Syt22 (Fig. 2 G ------- COMMENT: b793085c4da3e210 25 jS+1UMdqmCQC1+HmSoJ6u7jdcQE Indeed, arf6(Q75L)-mNG localized to nodes even in syt22Δ cells (Fig. 2 G) ------- COMMENT: b793085c4da3e210 26 OmTwcyFUDg76JYBuR8BDXZZ4IQw Ucp3-mNG localized to spots at the cell tips, which likely represent endocytic actin patches due to colocalization with actin patch component Pan1 (Fig. S2, E and F) ------- COMMENT: b793085c4da3e210 27 D7VyKS/klZhVP9VzlidS01xSfS0 The resulting arf6 alleles reduced node localization and instead enriched at the cytoplasm (Fig. S2, H and I) ------- COMMENT: b793085c4da3e210 28 D7VyKS/klZhVP9VzlidS01xSfS0 The resulting arf6 alleles reduced node localization and instead enriched at the cytoplasm (Fig. S2, H and I) ------- COMMENT: b793085c4da3e210 29 D7VyKS/klZhVP9VzlidS01xSfS0 The resulting arf6 alleles reduced node localization and instead enriched at the cytoplasm (Fig. S2, H and I) ------- COMMENT: b793085c4da3e210 30 aZrC6/2ZndEGwLDLGTC/mCDtghY rf6Δ cells had cytoplasmic Cdr2 clusters that were absent in wild-type cells (Figs. 3 A and S2 J), indicating defects in cortical anchoring. ------- COMMENT: b793085c4da3e210 31 uFNWlXXjbIH8CcsdEVCra2zLljY Modified form is indirect because GDP bound does not localize to nodes) "These defects indicate that Arf6 anchors Cdr2 stably at nodes, meaning that Arf6 and Cdr2 reciprocally promote each other’s node localization." ------- COMMENT: b793085c4da3e210 32 9TNqLqqIytTmRSmZB4aSx+Uhedw o explain this connection, we examined the localization of Wee1 and Cdr1 at cortical nodes in arf6Δ mutants. Wee1 localized to nodes in arf6Δ, but Cdr1 did not (Fig. 3, D and E; and Fig. S3 D) ------- COMMENT: b793085c4da3e210 33 llB+5VfymfwIODoN5fV+03gThZU We combined arf6Δ with the mid1(400–450Δ) mu- tant that cannot bind Cdr2. In the resulting cells, Cdr2 was ab- sent from the cell cortex and formed large cytoplasmic puncta (Figs. 4 A and S2 J). ------- COMMENT: b793085c4da3e210 34 xRismk/xP7L7pGbNTD03hlu3AT8 Thus, Arf6 and Mid1 are partially over- lapping anchors for Cdr2 nodes. ------- COMMENT: b793085c4da3e210 35 D7VyKS/klZhVP9VzlidS01xSfS0 The resulting arf6 alleles reduced node localization and instead enriched at the cytoplasm (Fig. S2, H and I) ------- COMMENT: b793085c4da3e210 36 FVTXFC+kjqYSEsJPYR4OF1zjgB8 (comment: CHECK actually mid1-Nter) ------- COMMENT: b793085c4da3e210 37 IvEsZdmM73KewvsBA9sgDeCJrO0 All three double mutants exhibited synthetic defects in cell growth and cytokinesis as judged by tilted and disorganized septa (Fig. 5, C–E; and Fig. S3 M). ------- COMMENT: b793085c4da3e210 38 IvEsZdmM73KewvsBA9sgDeCJrO0 All three double mutants exhibited synthetic defects in cell growth and cytokinesis as judged by tilted and disorganized septa (Fig. 5, C–E; and Fig. S3 M). ------- COMMENT: b793085c4da3e210 39 IvEsZdmM73KewvsBA9sgDeCJrO0 All three double mutants exhibited synthetic defects in cell growth and cytokinesis as judged by tilted and disorganized septa (Fig. 5, C–E; and Fig. S3 M). ------- COMMENT: b7a6635419d42c62 12 VEzgSNeYPTS1+7IicDhoe4IdoNg (comment: CHECK transmembrane import into Golgi lumen) ------- COMMENT: b7a6635419d42c62 13 VEzgSNeYPTS1+7IicDhoe4IdoNg (comment: CHECK transmembrane import into Golgi lumen) ------- COMMENT: b7b22ba04350037e 7 aMijiXSoe1zanhlA4Bpw3nE91ug (comment: CHECK abolished,) fig1d ------- COMMENT: b7b22ba04350037e 8 aMijiXSoe1zanhlA4Bpw3nE91ug (comment: CHECK abolished,) fig1d ------- COMMENT: b7b22ba04350037e 9 aMijiXSoe1zanhlA4Bpw3nE91ug (comment: abolished CHECK,) fig1d ------- COMMENT: b7b22ba04350037e 10 aMijiXSoe1zanhlA4Bpw3nE91ug (comment: CHECK abolished,) fig1d ------- COMMENT: b7b22ba04350037e 11 aMijiXSoe1zanhlA4Bpw3nE91ug (comment: CHECK abolished,) fig1d ------- COMMENT: b7b22ba04350037e 12 aMijiXSoe1zanhlA4Bpw3nE91ug (comment: CHECK abolished,) fig1d ------- COMMENT: b7b22ba04350037e 35 XzIRLy1uc8Ira/2EYG/TUGC1zTs fig S10 ------- COMMENT: b7c7becd0bc7a254 2 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: b7c7becd0bc7a254 3 gDzjkryVoq/9jpcrPAkmZRSDBdY Figure2 ------- COMMENT: b7c7becd0bc7a254 6 23A7gKYH++mwxpDaLPxFOcAYx6Y fig 3c ------- COMMENT: b7c7becd0bc7a254 7 23A7gKYH++mwxpDaLPxFOcAYx6Y fig 3c ------- COMMENT: b7c7becd0bc7a254 11 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: b7c7becd0bc7a254 13 3hiic6lToU1GFVpDs58mhh2ZFg0 (comment: dominent negative effect) ------- COMMENT: b7c7becd0bc7a254 15 3hiic6lToU1GFVpDs58mhh2ZFg0 (comment: dominent negative effect) ------- COMMENT: b7c7becd0bc7a254 16 3hiic6lToU1GFVpDs58mhh2ZFg0 (comment: dominent negative effect) ------- COMMENT: b7c7becd0bc7a254 18 v1uabuLlf/voX0ZycUM0lzta+CY Figure 6A) ------- COMMENT: b7c7becd0bc7a254 19 v1uabuLlf/voX0ZycUM0lzta+CY Figure 6A) ------- COMMENT: b7c7becd0bc7a254 20 /JKH6pe5km+QScxDnXVDLMI7U/8 Pic1– 765-924, which lacks the IN box, failed to bind Ark1p, ------- COMMENT: b7d7c9ccb439768b 1 mG7oAC3qgmyIMSdyl5Wb55m3k4E 3 sites in N-terminus (1-75) and 7 in C-terminus (1221-1485), but positions not determined ------- COMMENT: b7d7c9ccb439768b 3 9+H/QoY0IKkctWuKw7SpYakdwYg phosphorylated and dephosphorylated forms both active; no PR col 17 because no evidence that dephosphorylated form is physiologically relevant (dephosphorylated in vitro) ------- COMMENT: b7d8b3c8af45db70 2 4TiyK0t5N/MB/LpQQ3rFGLFQMIY Fig 1a ------- COMMENT: b7d8b3c8af45db70 3 4TiyK0t5N/MB/LpQQ3rFGLFQMIY Fig 1a ------- COMMENT: b7d8b3c8af45db70 4 oZkKMTo18oQzVCp/R71Js7/i4Ws Figs 1B, 1D, 1F, S1, S2 ------- COMMENT: b7d8b3c8af45db70 5 teYCJPyROvA42r3qb7yXpSZWBfI Figs 1C, S1 ------- COMMENT: b7d8b3c8af45db70 7 aOqYL7F2KV5d9E4mVC2uoiGmluk Figure 1E ------- COMMENT: b7d8b3c8af45db70 8 aOqYL7F2KV5d9E4mVC2uoiGmluk Figure 1E ------- COMMENT: b7d8b3c8af45db70 9 aOqYL7F2KV5d9E4mVC2uoiGmluk Figure 1E ------- COMMENT: b7d8b3c8af45db70 10 aOqYL7F2KV5d9E4mVC2uoiGmluk Figure 1E ------- COMMENT: b7d8b3c8af45db70 11 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: b7d8b3c8af45db70 12 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: b7d8b3c8af45db70 13 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: b7d8b3c8af45db70 14 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: b7d8b3c8af45db70 15 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: b7d8b3c8af45db70 16 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: b7d8b3c8af45db70 17 umtfEjFEgMoOIO8klisUmzKDahA (comment: CHECK homodimer) ------- COMMENT: b7d8b3c8af45db70 18 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: b8319e8f5cb44f5a 21 Ui+pYH5NXyCLtRLY+zUxXebTCbo Our data only demonstrate that this true for RNA Polymerase III(comment: // MOVED UP TO 'REGULATION' FROM NEG REG BASED ON NEW PUBLICATION) ------- COMMENT: b8319e8f5cb44f5a 30 lQJbAZldxmWYLO8BoozRnFzYapE (comment: CHECK SO:0001272 = tRNA gene) ------- COMMENT: b8319e8f5cb44f5a 35 lQJbAZldxmWYLO8BoozRnFzYapE (comment: CHECK SO:0001272 = tRNA gene) ------- COMMENT: b83f0d4242c0daee 21 SJZwE+cEKRfxSTs89a5M/2RoD5A (comment: binding by Pab2) ------- COMMENT: b843706c830e7b7d 2 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: b843706c830e7b7d 4 GefMojy64qknFm9NTEYbHJ35DFQ (comment: CHECK COINCIDENT WITH 5S_rRNA_gene NTR https://github.com/The-Sequence-Ontology/SO-Ontologies/issues/472) ------- COMMENT: b843706c830e7b7d 5 sTtyXsbNQf4o07hTm5NnGTdFN7o fig 1b We have shown using ChIP-seq experiments that Sen1 associates with all types of RNA polymerase III-transcribed genes. This includes tRNA_genes, 5S rRNA_genes, snu6 and srp7 but not the TFIIIC-bound COC sites. ------- COMMENT: b843706c830e7b7d 7 daXLabciTfcn/Atql2a864yXpVY fig 1A and F ------- COMMENT: b843706c830e7b7d 9 IQKQhINWCJAwlAnMdNoemo+E1nc AL fig 4. and 5c (comment: vincent: We have assayed the presence of read-through transcripts at SPATRNAPRO.02, SPCTRNAARG.10, SPBTRNATYR.04, SPBTRNAARG.05, SPCTRNASER.09, SPCTRNATHR.10 using strand-specific RT-qPCR. We also used Northern blots and 3' RACE to confirm the presence of read-through transcripts at SPATRNAPRO.02.) ------- COMMENT: b843706c830e7b7d 11 jGneieKYmNP5Nc/4F0sW/azvHLI ChIP-qPCR of Dbl8 indicates that Dbl8 is enriched at the rDNA and at highly-expressed RNAPII-transcribed genes ------- COMMENT: b843706c830e7b7d 12 rdiDRTgVlGAsDn5hEBWc4UOQrxk Fig. 2c, 5d ------- COMMENT: b843706c830e7b7d 40 coBTmtFSwuxkBMiY8P2UDllndpE Fig. 2b ------- COMMENT: b843706c830e7b7d 41 coBTmtFSwuxkBMiY8P2UDllndpE Fig. 2b ------- COMMENT: b843706c830e7b7d 42 coBTmtFSwuxkBMiY8P2UDllndpE Fig. 2b ------- COMMENT: b843706c830e7b7d 43 coBTmtFSwuxkBMiY8P2UDllndpE Fig. 2b ------- COMMENT: b843706c830e7b7d 45 coBTmtFSwuxkBMiY8P2UDllndpE Fig. 2b ------- COMMENT: b843706c830e7b7d 46 coBTmtFSwuxkBMiY8P2UDllndpE Fig. 2b ------- COMMENT: b843706c830e7b7d 47 coBTmtFSwuxkBMiY8P2UDllndpE Fig. 2b ------- COMMENT: b843706c830e7b7d 48 coBTmtFSwuxkBMiY8P2UDllndpE Fig. 2b ------- COMMENT: b843706c830e7b7d 50 d95o2uzYI7q7tY7bHI4U1xBug7s Fig. 3 ------- COMMENT: b843706c830e7b7d 51 d95o2uzYI7q7tY7bHI4U1xBug7s Fig. 3 ------- COMMENT: b843706c830e7b7d 54 G2RTiSRzpGfQc3LUXrBavCAxZHo Fig. 4 ------- COMMENT: b843706c830e7b7d 55 G2RTiSRzpGfQc3LUXrBavCAxZHo Fig. 4 ------- COMMENT: b843706c830e7b7d 56 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: b843706c830e7b7d 58 guzcRi9yglT3nujLAc8VzXSeriE AL fig 4. (comment: vincent: We have assayed the presence of read-through transcripts at SPATRNAPRO.02, SPCTRNAARG.10, SPBTRNATYR.04, SPBTRNAARG.05, SPCTRNASER.09, SPCTRNATHR.10 using strand-specific RT-qPCR. We also used Northern blots and 3' RACE to confirm the presence of read-through transcripts at SPATRNAPRO.02.) ------- COMMENT: b843706c830e7b7d 66 ha8vF+G1Juia4S47i+tjmHxl6wM (comment: CHECK coincident with 5S_rRNA_gene NTR https://github.com/The-Sequence-Ontology/SO-Ontologies/issues/472) ------- COMMENT: b843706c830e7b7d 67 ha8vF+G1Juia4S47i+tjmHxl6wM (comment: CHECK coincident with 5S_rRNA_gene NTR https://github.com/The-Sequence-Ontology/SO-Ontologies/issues/472) ------- COMMENT: b843706c830e7b7d 68 ha8vF+G1Juia4S47i+tjmHxl6wM (comment: CHECK coincident with 5S_rRNA_gene NTR https://github.com/The-Sequence-Ontology/SO-Ontologies/issues/472) ------- COMMENT: b843706c830e7b7d 69 ha8vF+G1Juia4S47i+tjmHxl6wM (comment: CHECK coincident with 5S_rRNA_gene NTR https://github.com/The-Sequence-Ontology/SO-Ontologies/issues/472) ------- COMMENT: b843706c830e7b7d 72 Jb0MgAr1rc/YVxwosV1AmFAQbi4 Fig. ev1 ------- COMMENT: b85e8d183eac4d18 1 crYwDpB/FaOkBxEWs4Q1FcttJPc In contrast, Tet-based overexpression of cdr2(E177A) increased the size of dividing cells, consistent with dominant-negative effects for ------- COMMENT: b85e8d183eac4d18 2 LGqHrNz4a1tYQEmFtB4kcPgjDP0 Addition of Tet to PTet-cdr2 cells caused a marked and significant decrease in cell length at division (Fig. 2, A and B) ------- COMMENT: b85e8d183eac4d18 4 YnI3sHg7ttDSlmqEiY2AgChnjTc figure3 ------- COMMENT: b85e8d183eac4d18 5 YnI3sHg7ttDSlmqEiY2AgChnjTc figure3 ------- COMMENT: b85e8d183eac4d18 14 GKA/K6O+Zo9LG5QfLQyzZqdSvfI neither Cdr2(1–330) nor Cdr2(1–590) truncations reduced cell size or formed cytoplasmic clusters (Fig. 7, B–D) despite expression of all constructs to similar levels (Fig. S3A). ------- COMMENT: b85e8d183eac4d18 15 GKA/K6O+Zo9LG5QfLQyzZqdSvfI neither Cdr2(1–330) nor Cdr2(1–590) truncations reduced cell size or formed cytoplasmic clusters (Fig. 7, B–D) despite expression of all constructs to similar levels (Fig. S3A). ------- COMMENT: b85e8d183eac4d18 18 0Bm21Lr65Zv3m/7Y7u1FC6nsHYk However, induction of PTet-cdr2 in cdr1Δ cdr2Δ cells still reduced cell size, whereas PTet-cdr2(E177A) had no effect (Fig. 2, C and D). ------- COMMENT: b85e8d183eac4d18 19 PPLZdU1gCpWVbswFK7iDV8veQDo (comment: (vw: did not reduce cell size further) EPISTATIC) Consistent with this prediction, PTet-cdr2 did not reduce cell J. Biol. Chem. (2023) 299(2) 102831 3 Regulation of cell size and Wee1 by elevated levels of Cdr2 size in the temperature-sensitive wee1-50 mutant grown at 36 C (Fig. 2E). ------- COMMENT: b85e8d183eac4d18 20 l0HgujgCz3Z60bMhnNROD4Lz4SY (comment: dosage dependent) (We conclude that increased levels of Cdr2 cause hyperphosphorylation of Wee1 leading to reduced cell size at division.) ------- COMMENT: b85e8d183eac4d18 21 wki8bhojQIh2Y6KcPy8XPeri3Vk (comment: CHECK HYPERPHOSPHORYLATED WEE1 (get identifier)) ------- COMMENT: b86c94d0c7fb4dcc 17 u9d2g80JU8xlnVoocG2FiRONoa4 septation index increased gradually over time ------- COMMENT: b86c94d0c7fb4dcc 19 juM1NipDMuG1ic/+yqI+GQ4es5w septation index constantly high ------- COMMENT: b86c94d0c7fb4dcc 24 D9bSmF80Z9UBbxwamaJpyU4YCiA decreased septum closure ------- COMMENT: b86c94d0c7fb4dcc 26 QH7b+lVvCDF5aIEUvlSdkmLzkn0 decreased/delayed septum closure ------- COMMENT: b86c94d0c7fb4dcc 37 fVOfUBTQMvTH6zSm0hbPmpzqjUU normal localization in several mutants indicates that Sec3 localization is independent of exocytosis and vesicle-mediated transport along microtubules ------- COMMENT: b86c94d0c7fb4dcc 75 8mOmgWbQu4OnPi0OdTnGACvCcfY At the end of ring constriction Filamentous projections from the unclosed ring toward the cytoplasm ------- COMMENT: b86c94d0c7fb4dcc 93 MFpMkifcG/1GDhzoJuUl6Kc/qhQ (comment: Assayed by FM4-64 uptake) ------- COMMENT: b86c94d0c7fb4dcc 103 sWC8zdf96sXZNJVoCi6kxS3b3Rg Weak actin cables ------- COMMENT: b86c94d0c7fb4dcc 108 nIyXlEC9yCWDynzbVsIUz9fPWHQ (comment: assayed at 32C, which is semi-permissive for sec3-913) ------- COMMENT: b897e8a27ea98541 6 S/za2QaMm2Sqlv89aYDSttkjPsw Figure 2, E and F). We concluded that Pkd2 is calcium-permeable under the mechanical stimulus of membrane stretching. ------- COMMENT: b897e8a27ea98541 8 6OYajUruRnBdyO9MvmTUUEnbh6o Supplemental Figure S5A). We concluded that the calcium-permeable Pkd2 primarily localizes to the plasma membrane. ------- COMMENT: b897e8a27ea98541 9 4eAihL3DHkuZ4+HCIYZkp12ZQS4 At 36°C, the average calcium level of pkd2-B42 cells was 34% lower than that of wild type cells (Figure 3, C and D). ------- COMMENT: b897e8a27ea98541 10 +C5rf66tMztbyaxpwhXFuFOz/RA The peak amplitude of the cal- cium spikes in pkd2-B42 cells was similarly reduced by 62% (Figure 4D). ------- COMMENT: b8a8ea77ee8fb109 5 xxZXO8cSdsKiDRXAFplchnFrdBs figure5a-c ------- COMMENT: b8a8ea77ee8fb109 6 xxZXO8cSdsKiDRXAFplchnFrdBs figure5a-c ------- COMMENT: b8a8ea77ee8fb109 7 xxZXO8cSdsKiDRXAFplchnFrdBs figure5a-c ------- COMMENT: b8a8ea77ee8fb109 8 xxZXO8cSdsKiDRXAFplchnFrdBs figure5a-c ------- COMMENT: b8a8ea77ee8fb109 9 xxZXO8cSdsKiDRXAFplchnFrdBs figure5a-c ------- COMMENT: b8a8ea77ee8fb109 10 xxZXO8cSdsKiDRXAFplchnFrdBs figure5a-c ------- COMMENT: b8a8ea77ee8fb109 11 xxZXO8cSdsKiDRXAFplchnFrdBs figure5a-c ------- COMMENT: b8a8ea77ee8fb109 12 xxZXO8cSdsKiDRXAFplchnFrdBs figure5a-c ------- COMMENT: b8a8ea77ee8fb109 13 5J0xC+Cr4WCT7Cl8wvoZLszeMDE Fig. 6d ------- COMMENT: b8a8ea77ee8fb109 14 5J0xC+Cr4WCT7Cl8wvoZLszeMDE Fig. 6d ------- COMMENT: b8a8ea77ee8fb109 15 xEzj2OkseCGyqne3MK+npq5GJ1Q Fig. 6b, c ------- COMMENT: b8a8ea77ee8fb109 16 xEzj2OkseCGyqne3MK+npq5GJ1Q Fig. 6b, c ------- COMMENT: b8a8ea77ee8fb109 22 xEzj2OkseCGyqne3MK+npq5GJ1Q Fig. 6b, c ------- COMMENT: b8a8ea77ee8fb109 23 xEzj2OkseCGyqne3MK+npq5GJ1Q Fig. 6b, c ------- COMMENT: b8a8ea77ee8fb109 24 xEzj2OkseCGyqne3MK+npq5GJ1Q Fig. 6b, c ------- COMMENT: b8a8ea77ee8fb109 25 5J0xC+Cr4WCT7Cl8wvoZLszeMDE Fig. 6d ------- COMMENT: b9099784b5cb5119 12 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: b9099784b5cb5119 13 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: b9099784b5cb5119 14 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: b91dabbdf32d8353 1 ivfFXUKOx0dF2nU7ASfHKaQSBbs (comment: CHECK activated_by(CHEBI:18420)| inhibited_by(CHEBI:29108)) ------- COMMENT: b924eeaf3c11e580 1 varpjo2wsNU1aMvzDW1zH+Bm7TY Phenotype complementation by human RAD23A ------- COMMENT: b94d5bebcfaa526b 1 Xq1G0BPuKaP4Fkg+RIIjHxi2Iek Table1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 2 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 3 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 4 rGC7LLxeYyDLciKUnIOO7FO6p6Q (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 5 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 6 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 7 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 8 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 9 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 10 FCc+7Rhce8w4Gon4iI9yZjbCkfc Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 11 FCc+7Rhce8w4Gon4iI9yZjbCkfc Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 12 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 13 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 14 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 15 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 16 hDStnqK3+O9e7Y++NP5IoqS5Yck (comment: mutant gene expressed from multicopy plasmid pIRT2 has dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 17 MjRArSBIFYI2KTFr+BMXpAPU32Y Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 18 MjRArSBIFYI2KTFr+BMXpAPU32Y Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 19 MjRArSBIFYI2KTFr+BMXpAPU32Y Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 20 MjRArSBIFYI2KTFr+BMXpAPU32Y Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 21 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 22 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 23 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 24 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 25 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 26 apkNs7B3WXotdEt3N1VEIorC+ZM Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 suppresses the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 27 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 28 apkNs7B3WXotdEt3N1VEIorC+ZM Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 suppresses the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 29 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 30 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 31 UWNbRZ1LYAlSCKx8bmUfXffLNYw Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 does not suppress the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 32 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 34 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 35 apkNs7B3WXotdEt3N1VEIorC+ZM Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 suppresses the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 36 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 37 JEp1gcdTeUmWxwDN9VWyUuTs9Cw Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 partially supresses the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 38 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 39 UWNbRZ1LYAlSCKx8bmUfXffLNYw Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 does not suppress the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 40 rGC7LLxeYyDLciKUnIOO7FO6p6Q (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 42 rGC7LLxeYyDLciKUnIOO7FO6p6Q (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 43 rGC7LLxeYyDLciKUnIOO7FO6p6Q (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 44 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 45 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 46 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 48 O7kyX9621CKDGvn7OyB6UpomXjs Table 1, Fig 2 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 49 UWNbRZ1LYAlSCKx8bmUfXffLNYw Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 does not suppress the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 50 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 51 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 52 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 53 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 54 5etvsKswGc7jss7+zDEKW7fxqEE Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 partially suppresses the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 55 rGC7LLxeYyDLciKUnIOO7FO6p6Q (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 56 apkNs7B3WXotdEt3N1VEIorC+ZM Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 suppresses the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 57 rGC7LLxeYyDLciKUnIOO7FO6p6Q (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 58 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 59 rGC7LLxeYyDLciKUnIOO7FO6p6Q (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 60 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 61 UWNbRZ1LYAlSCKx8bmUfXffLNYw Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 does not suppress the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 62 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 63 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 64 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 65 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 66 apkNs7B3WXotdEt3N1VEIorC+ZM Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 suppresses the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 67 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 68 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 69 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 70 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 71 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 72 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 74 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 75 kXJEB0haIu1r1taDdQLyJ9Ra+2c Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 surpresses the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 76 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 77 8wPAAeSPKIkm4DUAy4ajPdezfDQ Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 partially supresses the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 78 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 79 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 80 oIBIJB6kz5Sd9wJNhsWGU3ldv5k Table1 (comment: mutant gene expressed from multicopy plasmid pIRT2 does not suppress the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 81 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 82 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 83 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 84 maAqem3f5icp0s7D9c8nykURIME Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 supresses the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 85 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 86 PBuHjV5187ro1q3DtlmhphMk8Os Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 does not supress the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 87 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 88 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 89 P/079sT5xfQd7kfFVfvaT3TT+sU Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 does not supress the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 90 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 91 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 92 maAqem3f5icp0s7D9c8nykURIME Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 supresses the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 93 rGC7LLxeYyDLciKUnIOO7FO6p6Q (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 94 P/079sT5xfQd7kfFVfvaT3TT+sU Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 does not supress the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 95 rGC7LLxeYyDLciKUnIOO7FO6p6Q (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 96 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 98 P/079sT5xfQd7kfFVfvaT3TT+sU Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 does not supress the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 99 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 100 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 101 maAqem3f5icp0s7D9c8nykURIME Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 supresses the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 102 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 103 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 105 maAqem3f5icp0s7D9c8nykURIME Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 supresses the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 106 rGC7LLxeYyDLciKUnIOO7FO6p6Q (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 107 FCc+7Rhce8w4Gon4iI9yZjbCkfc Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 108 PG3J1gH3i1kBwLqKKGb3hxfa1Eg (comment: mutant expressed from multi copy plasmid has dominant negative phenotype) Table 1 ------- COMMENT: b94d5bebcfaa526b 109 PG3J1gH3i1kBwLqKKGb3hxfa1Eg (comment: mutant expressed from multi copy plasmid has dominant negative phenotype) Table 1 ------- COMMENT: b94d5bebcfaa526b 110 2lfIDgHDS1fCcQ6dbTiZ420bKzo Table 1 (comment: mutant expressed from multi copy plasmid has dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 111 2lfIDgHDS1fCcQ6dbTiZ420bKzo Table 1 (comment: mutant expressed from multi copy plasmid has dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 113 FCc+7Rhce8w4Gon4iI9yZjbCkfc Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 114 PG3J1gH3i1kBwLqKKGb3hxfa1Eg (comment: mutant expressed from multi copy plasmid has dominant negative phenotype) Table 1 ------- COMMENT: b94d5bebcfaa526b 115 PG3J1gH3i1kBwLqKKGb3hxfa1Eg (comment: mutant expressed from multi copy plasmid has dominant negative phenotype) Table 1 ------- COMMENT: b94d5bebcfaa526b 116 PG3J1gH3i1kBwLqKKGb3hxfa1Eg (comment: mutant expressed from multi copy plasmid has dominant negative phenotype) Table 1 ------- COMMENT: b94d5bebcfaa526b 117 PG3J1gH3i1kBwLqKKGb3hxfa1Eg (comment: mutant expressed from multi copy plasmid has dominant negative phenotype) Table 1 ------- COMMENT: b94d5bebcfaa526b 118 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 119 maAqem3f5icp0s7D9c8nykURIME Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 supresses the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 120 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 121 PBuHjV5187ro1q3DtlmhphMk8Os Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 does not supress the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 122 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 123 P/079sT5xfQd7kfFVfvaT3TT+sU Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 does not supress the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 124 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 125 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 126 maAqem3f5icp0s7D9c8nykURIME Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 supresses the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 127 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 128 P/079sT5xfQd7kfFVfvaT3TT+sU Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 does not supress the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 129 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 130 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 131 P/079sT5xfQd7kfFVfvaT3TT+sU Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 does not supress the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 132 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 133 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 134 maAqem3f5icp0s7D9c8nykURIME Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 supresses the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 135 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 136 8wPAAeSPKIkm4DUAy4ajPdezfDQ Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 partially supresses the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 138 rGC7LLxeYyDLciKUnIOO7FO6p6Q (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 139 DDfQAuJvoLNwJTfbrLg3sq5EE6g Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 give partial suppression) ------- COMMENT: b94d5bebcfaa526b 140 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 141 maAqem3f5icp0s7D9c8nykURIME Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 supresses the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 142 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 143 fh9leopNUg2p4g4F5Og3vEP5wL8 Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 does not suppress the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 144 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 145 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 146 PBuHjV5187ro1q3DtlmhphMk8Os Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 does not supress the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 147 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 148 hDStnqK3+O9e7Y++NP5IoqS5Yck (comment: mutant gene expressed from multicopy plasmid pIRT2 has dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 149 MjRArSBIFYI2KTFr+BMXpAPU32Y Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 150 MjRArSBIFYI2KTFr+BMXpAPU32Y Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 151 MjRArSBIFYI2KTFr+BMXpAPU32Y Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 152 MjRArSBIFYI2KTFr+BMXpAPU32Y Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 153 FCc+7Rhce8w4Gon4iI9yZjbCkfc Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 154 FCc+7Rhce8w4Gon4iI9yZjbCkfc Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 155 FCc+7Rhce8w4Gon4iI9yZjbCkfc Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 156 FCc+7Rhce8w4Gon4iI9yZjbCkfc Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 157 FCc+7Rhce8w4Gon4iI9yZjbCkfc Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 158 FCc+7Rhce8w4Gon4iI9yZjbCkfc Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 159 eeMR8pNoc2HPVBYIrOKJZFH9zIU (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 160 eeMR8pNoc2HPVBYIrOKJZFH9zIU (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 161 eeMR8pNoc2HPVBYIrOKJZFH9zIU (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 162 FCc+7Rhce8w4Gon4iI9yZjbCkfc Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 164 eeMR8pNoc2HPVBYIrOKJZFH9zIU (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 165 FCc+7Rhce8w4Gon4iI9yZjbCkfc Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 166 FCc+7Rhce8w4Gon4iI9yZjbCkfc Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 167 eeMR8pNoc2HPVBYIrOKJZFH9zIU (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 168 eeMR8pNoc2HPVBYIrOKJZFH9zIU (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 169 FCc+7Rhce8w4Gon4iI9yZjbCkfc Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 170 FCc+7Rhce8w4Gon4iI9yZjbCkfc Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 171 FCc+7Rhce8w4Gon4iI9yZjbCkfc Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 172 FCc+7Rhce8w4Gon4iI9yZjbCkfc Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 173 FCc+7Rhce8w4Gon4iI9yZjbCkfc Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 174 FCc+7Rhce8w4Gon4iI9yZjbCkfc Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 has a dominant negative phenotype) ------- COMMENT: b94d5bebcfaa526b 175 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 176 maAqem3f5icp0s7D9c8nykURIME Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 supresses the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 177 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 178 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 179 GxSMgFEkjR5fzN9lpiUOiG4wn5I Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 no suppression) ------- COMMENT: b94d5bebcfaa526b 180 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 181 P/079sT5xfQd7kfFVfvaT3TT+sU Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 does not supress the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 182 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 183 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 184 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 185 GxSMgFEkjR5fzN9lpiUOiG4wn5I Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 no suppression) ------- COMMENT: b94d5bebcfaa526b 186 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 187 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 188 GxSMgFEkjR5fzN9lpiUOiG4wn5I Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 no suppression) ------- COMMENT: b94d5bebcfaa526b 189 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 190 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 191 GxSMgFEkjR5fzN9lpiUOiG4wn5I Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 no suppression) ------- COMMENT: b94d5bebcfaa526b 192 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 193 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 194 GxSMgFEkjR5fzN9lpiUOiG4wn5I Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 no suppression) ------- COMMENT: b94d5bebcfaa526b 195 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 196 rGC7LLxeYyDLciKUnIOO7FO6p6Q (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 197 rGC7LLxeYyDLciKUnIOO7FO6p6Q (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 198 rGC7LLxeYyDLciKUnIOO7FO6p6Q (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 200 wN3emR+KCPRdF68QVi/OeC9bGz8 Fig 4 (comment: CHECK nmt1 promoter ON) ------- COMMENT: b94d5bebcfaa526b 201 wN3emR+KCPRdF68QVi/OeC9bGz8 Fig 4 (comment: CHECK nmt1 promoter ON) ------- COMMENT: b94d5bebcfaa526b 202 TkqTHZpUmbatp28HAqR4ZblN7LE Table 1 (comment: CHECK nmt1 promoter ON) ------- COMMENT: b94d5bebcfaa526b 203 TkqTHZpUmbatp28HAqR4ZblN7LE Table 1 (comment: CHECK nmt1 promoter ON) ------- COMMENT: b94d5bebcfaa526b 204 Tudwsk7CLGZe3Zr66kndrR3a8wg Fig 4, Table 1 (comment: CHECK nmt1 promoter ON) ------- COMMENT: b94d5bebcfaa526b 205 iEXTE3t2h+QviH3i7Yzv4fPT9dg Fig 4, Table 1 (comment: CHECK nmt1 promoter ON) ------- COMMENT: b94d5bebcfaa526b 206 pRi6XfyNUeAFXuitAfj2I+/1tl8 Table 1, Fig 4 (comment: CHECK nmt1 promoter ON) ------- COMMENT: b94d5bebcfaa526b 207 pRi6XfyNUeAFXuitAfj2I+/1tl8 Table 1, Fig 4 (comment: CHECK nmt1 promoter ON) ------- COMMENT: b94d5bebcfaa526b 208 TkqTHZpUmbatp28HAqR4ZblN7LE Table 1 (comment: CHECK nmt1 promoter ON) ------- COMMENT: b94d5bebcfaa526b 209 TkqTHZpUmbatp28HAqR4ZblN7LE Table 1 (comment: CHECK nmt1 promoter ON) ------- COMMENT: b94d5bebcfaa526b 210 TkqTHZpUmbatp28HAqR4ZblN7LE Table 1 (comment: CHECK nmt1 promoter ON) ------- COMMENT: b94d5bebcfaa526b 211 TkqTHZpUmbatp28HAqR4ZblN7LE Table 1 (comment: CHECK nmt1 promoter ON) ------- COMMENT: b94d5bebcfaa526b 212 TkqTHZpUmbatp28HAqR4ZblN7LE Table 1 (comment: CHECK nmt1 promoter ON) ------- COMMENT: b94d5bebcfaa526b 213 TkqTHZpUmbatp28HAqR4ZblN7LE Table 1 (comment: CHECK nmt1 promoter ON) ------- COMMENT: b94d5bebcfaa526b 214 T8sJkJwX7gydt3yNOcx/yZgRkr0 Fig 5 (comment: CHECK nmt1 promoter ON) ------- COMMENT: b94d5bebcfaa526b 215 T8sJkJwX7gydt3yNOcx/yZgRkr0 Fig 5 (comment: CHECK nmt1 promoter ON) ------- COMMENT: b94d5bebcfaa526b 216 T8sJkJwX7gydt3yNOcx/yZgRkr0 Fig 5 (comment: CHECK nmt1 promoter ON) ------- COMMENT: b94d5bebcfaa526b 217 S4AaueSKbuLq4HzPosl5YNiMFhs Table 1, Fig 4 (comment: CHECK nmt1 ON) ------- COMMENT: b94d5bebcfaa526b 218 OolTckGy6keoiRYVDMzDlG4vHD0 Cdc2 is only mildly over expressed as it is expressed from a multi copy plasmid pIRT2. This is much lower over expression than from the nmt1 promoter and for all the other annotations were the cdc2 mutant is expressed from pIRT2 I have said 'unknown' for expression level ------- COMMENT: b94d5bebcfaa526b 219 OolTckGy6keoiRYVDMzDlG4vHD0 Cdc2 is only mildly over expressed as it is expressed from a multi copy plasmid pIRT2. This is much lower over expression than from the nmt1 promoter and for all the other annotations were the cdc2 mutant is expressed from pIRT2 I have said 'unknown' for expression level ------- COMMENT: b94d5bebcfaa526b 220 0E+9DsY3HC5K9k8GgUbWA65AAVY Fig 5 increased duration of G1 phase ------- COMMENT: b94d5bebcfaa526b 221 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 222 wiMnvwtmCq56dlxDWQiiTO+Crvo Table 1, Fig 2 (comment: mutant gene expressed from multicopy plasmid pIRT2 suppresses the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 223 k9maCEL00tue29mSQzA76G7f1tU Table 1, Fig2 (comment: mutant gene expressed from multicopy plasmid pIRT2 partially suppresses the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 224 1L2lkcQnD4EzFp6Xl4Go/P9W1Eo Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 225 DLk7VYxntIpn4BI4I4wpNLhlKl0 Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 226 PBuHjV5187ro1q3DtlmhphMk8Os Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 does not supress the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 227 nIQPYdNyLKzTTO/7dJyXNzJHygM Table 1 (comment: mutant expressed from multi copy plasmid pIRT2) ------- COMMENT: b94d5bebcfaa526b 228 P/079sT5xfQd7kfFVfvaT3TT+sU Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 does not supress the ts phenotype) ------- COMMENT: b94d5bebcfaa526b 229 P/079sT5xfQd7kfFVfvaT3TT+sU Table 1 (comment: mutant gene expressed from multicopy plasmid pIRT2 does not supress the ts phenotype) ------- COMMENT: b95bc9d658c77529 1 +CxsgMPRPJIluuHTFtSrEPgPrL0 (comment: CHECK activated_by FAD , inhibited_by L-valine) ------- COMMENT: b9761ecd7fdd2a70 4 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: b9761ecd7fdd2a70 21 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: b9761ecd7fdd2a70 22 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: b9792083a95e2fee 9 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: b9792083a95e2fee 10 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: b9792083a95e2fee 11 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: b9792083a95e2fee 12 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: b9792083a95e2fee 13 MRv4U0wptZfY8tXQDHMhzMNic0c figure 2B ------- COMMENT: b9792083a95e2fee 14 BrfIbYRWSQXRJuEBq4H5V6IodbY fig 2B ------- COMMENT: b9792083a95e2fee 15 o9LfcExe7QLxPGKhYiLbwsmshlo fig 2C ------- COMMENT: b9792083a95e2fee 16 BT7Zwp+szYIDVy+MBv3HVF2DLfE (comment: CHECK wt 68%) fig 2C ------- COMMENT: b9792083a95e2fee 17 7IzPVIuZhuKqIVjB4l/cExHiwb8 fig 2D ------- COMMENT: b9792083a95e2fee 18 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: b9792083a95e2fee 19 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: b9792083a95e2fee 20 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: b9792083a95e2fee 21 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: b9792083a95e2fee 22 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: b9792083a95e2fee 23 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: b9792083a95e2fee 24 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: b9792083a95e2fee 25 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: b9792083a95e2fee 26 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: b9792083a95e2fee 27 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: b9792083a95e2fee 28 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: b9792083a95e2fee 29 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: b9792083a95e2fee 30 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: b9792083a95e2fee 31 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: b9792083a95e2fee 32 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: b9792083a95e2fee 33 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: b9792083a95e2fee 34 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: b9792083a95e2fee 35 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: b9792083a95e2fee 36 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: b9792083a95e2fee 37 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: b9792083a95e2fee 38 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: b9792083a95e2fee 39 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: b9792083a95e2fee 40 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: b9792083a95e2fee 41 jKE0HAk4aLf+MD8iL/svYPS8rHQ fig 8 ------- COMMENT: b985cb4ee3e632ef 2 khsisV/+a3EzkwQ6iLhnNm1HrpM (Fig. 6) ------- COMMENT: b985cb4ee3e632ef 4 Y8pqWYfUPfaXzWXbnDd55sL+ZA8 (Fig. 7) ------- COMMENT: b985cb4ee3e632ef 5 kuRdbpnFrU9sjksgW9GQwEQ1424 (Fig. 5) ------- COMMENT: b985cb4ee3e632ef 6 kuRdbpnFrU9sjksgW9GQwEQ1424 (Fig. 5) ------- COMMENT: b985cb4ee3e632ef 8 Y8pqWYfUPfaXzWXbnDd55sL+ZA8 (Fig. 7) ------- COMMENT: b985cb4ee3e632ef 9 3FcJuLRqm431ZQtCu9UjfiL0o/s (Fig. 7D) ------- COMMENT: b985cb4ee3e632ef 10 wFjaOMzh+P1XGYK6Z0f9ZyrlkH8 (Fig. 7E and F) ------- COMMENT: b985cb4ee3e632ef 11 caisk3ZmrdjW+p2nhfP0pw86f5c (Fig. S4) ------- COMMENT: b985cb4ee3e632ef 12 caisk3ZmrdjW+p2nhfP0pw86f5c (Fig. S4) ------- COMMENT: b985cb4ee3e632ef 13 caisk3ZmrdjW+p2nhfP0pw86f5c (Fig. S4) ------- COMMENT: b9dddd6cca4a0b44 7 GzQQRIirPoWD2NK+XSQSE9jSc3U (comment: CHECK in vitro) ------- COMMENT: b9fe9740c46775d4 38 AtaXr2CpThUVVP5n1hOmh6ZwIcY fig S1B ------- COMMENT: b9fe9740c46775d4 39 AtaXr2CpThUVVP5n1hOmh6ZwIcY fig S1B ------- COMMENT: b9fe9740c46775d4 40 AtaXr2CpThUVVP5n1hOmh6ZwIcY fig S1B ------- COMMENT: ba339daa4ef4e392 1 122e33HQFDM68v1qlUVm15pihr8 (Fig. 4B, 6A) ------- COMMENT: ba339daa4ef4e392 2 hPFNK9pXn9s6uyKJPTkg3DIM2XE (Fig. 6A) ------- COMMENT: ba339daa4ef4e392 3 khsisV/+a3EzkwQ6iLhnNm1HrpM (Fig. 6) ------- COMMENT: ba339daa4ef4e392 4 sUCS79Rs9xFD1wViTRlsgeGdNFs (Fig. 6B) ------- COMMENT: ba339daa4ef4e392 5 sUCS79Rs9xFD1wViTRlsgeGdNFs (Fig. 6B) ------- COMMENT: ba339daa4ef4e392 6 wnx2gS3IAKqzPkStlyJ0uF9GveM (Fig. 6C) ------- COMMENT: ba339daa4ef4e392 7 wnx2gS3IAKqzPkStlyJ0uF9GveM (Fig. 6C) ------- COMMENT: ba339daa4ef4e392 8 sUCS79Rs9xFD1wViTRlsgeGdNFs (Fig. 6B) ------- COMMENT: ba339daa4ef4e392 9 2SzzzO7fhvsfj6BW9v5jsOqwj/4 (Fig. S3) ------- COMMENT: ba339daa4ef4e392 10 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: ba339daa4ef4e392 11 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: ba339daa4ef4e392 12 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: ba339daa4ef4e392 13 DbVNgyu77u7yZqFAZtbNcJCtGLw (Table 1) ------- COMMENT: ba339daa4ef4e392 14 DbVNgyu77u7yZqFAZtbNcJCtGLw (Table 1) ------- COMMENT: ba339daa4ef4e392 15 DbVNgyu77u7yZqFAZtbNcJCtGLw (Table 1) ------- COMMENT: ba339daa4ef4e392 16 DbVNgyu77u7yZqFAZtbNcJCtGLw (Table 1) ------- COMMENT: ba339daa4ef4e392 17 DbVNgyu77u7yZqFAZtbNcJCtGLw (Table 1) ------- COMMENT: ba339daa4ef4e392 18 DbVNgyu77u7yZqFAZtbNcJCtGLw (Table 1) ------- COMMENT: ba339daa4ef4e392 19 DbVNgyu77u7yZqFAZtbNcJCtGLw (Table 1) ------- COMMENT: ba339daa4ef4e392 20 DbVNgyu77u7yZqFAZtbNcJCtGLw (Table 1) ------- COMMENT: ba339daa4ef4e392 21 DbVNgyu77u7yZqFAZtbNcJCtGLw (Table 1) ------- COMMENT: ba339daa4ef4e392 22 DbVNgyu77u7yZqFAZtbNcJCtGLw (Table 1) ------- COMMENT: ba339daa4ef4e392 23 DbVNgyu77u7yZqFAZtbNcJCtGLw (Table 1) ------- COMMENT: ba339daa4ef4e392 24 DbVNgyu77u7yZqFAZtbNcJCtGLw (Table 1) ------- COMMENT: ba339daa4ef4e392 25 DbVNgyu77u7yZqFAZtbNcJCtGLw (Table 1) ------- COMMENT: ba339daa4ef4e392 26 DbVNgyu77u7yZqFAZtbNcJCtGLw (Table 1) ------- COMMENT: ba339daa4ef4e392 27 DbVNgyu77u7yZqFAZtbNcJCtGLw (Table 1) ------- COMMENT: ba339daa4ef4e392 28 DbVNgyu77u7yZqFAZtbNcJCtGLw (Table 1) ------- COMMENT: ba339daa4ef4e392 29 DbVNgyu77u7yZqFAZtbNcJCtGLw (Table 1) ------- COMMENT: ba339daa4ef4e392 30 DbVNgyu77u7yZqFAZtbNcJCtGLw (Table 1) ------- COMMENT: ba339daa4ef4e392 31 DbVNgyu77u7yZqFAZtbNcJCtGLw (Table 1) ------- COMMENT: ba339daa4ef4e392 32 DbVNgyu77u7yZqFAZtbNcJCtGLw (Table 1) ------- COMMENT: ba339daa4ef4e392 33 DbVNgyu77u7yZqFAZtbNcJCtGLw (Table 1) ------- COMMENT: ba41ae11a8a2c405 8 BmtNzRDqmHt0WF/PYGklZv+14JI eaf1 binds ebp1 only in presence of ell1 ------- COMMENT: ba41ae11a8a2c405 9 TbLtotQfUNlq1vv30N3D/Z4OxhM Figure 5. ------- COMMENT: ba41ae11a8a2c405 10 TbLtotQfUNlq1vv30N3D/Z4OxhM Figure 5. ------- COMMENT: ba41ae11a8a2c405 11 TbLtotQfUNlq1vv30N3D/Z4OxhM Figure 5. ------- COMMENT: ba41ae11a8a2c405 12 5g806F7XKpqCFoxUOhLEcwm2r6M Figures 4, 5. ------- COMMENT: ba41ae11a8a2c405 13 5g806F7XKpqCFoxUOhLEcwm2r6M Figures 4, 5. ------- COMMENT: ba41ae11a8a2c405 14 5g806F7XKpqCFoxUOhLEcwm2r6M Figures 4, 5. ------- COMMENT: ba41ae11a8a2c405 15 r7Nmtq5JU66BtIy8TTq6Y/BxgHY also inferred from orthology, interactions, and chromatin localization (ChIP) ------- COMMENT: ba41ae11a8a2c405 16 r7Nmtq5JU66BtIy8TTq6Y/BxgHY also inferred from orthology, interactions, and chromatin localization (ChIP) ------- COMMENT: ba41ae11a8a2c405 17 r7Nmtq5JU66BtIy8TTq6Y/BxgHY also inferred from orthology, interactions, and chromatin localization (ChIP) ------- COMMENT: ba747e640ba07638 1 SduXTnOAE21zNDW28Ie9MiFbuuA Assayed protein is Ypt2. GFP-Ypt2 localization was delayed during meiosis I. Figure 2B and C ------- COMMENT: ba747e640ba07638 2 x9JGh8tYvzqhdS6zBeQwJWMYvGo Assayed protein is Spo13. Figure 2D ------- COMMENT: ba7771dd7ab8e04e 1 6/5jMi8gDChy3g1+6tMBmCoAm0k Indeed, the R288K and Q364R mutations in SpHCS confer diminished sensitivity to feedback inhibition by L-lysine in vitro and in vivo (Table 2 and Fig. 4) ------- COMMENT: ba7771dd7ab8e04e 2 6/5jMi8gDChy3g1+6tMBmCoAm0k Indeed, the R288K and Q364R mutations in SpHCS confer diminished sensitivity to feedback inhibition by L-lysine in vitro and in vivo (Table 2 and Fig. 4) ------- COMMENT: ba7ce39a2e9301af 1 B1iJywxRw+9C38lKvKPJol6LLi0 (comment: CHECK Sequence LVIAMDQLNL mentioned in the text) ------- COMMENT: ba7ce39a2e9301af 2 l6in7iPD2gBVzVMS2Uuo1lcns3g See Fig. 1 ------- COMMENT: ba8221c44a2afd95 1 MLEZbI3GohLIbRZnOtgOJ7IHSP0 Fig. 6b-d; Rad52 foci quantification, G2 arrested cells by cdc2-asM17, + Thiolutin ------- COMMENT: ba8221c44a2afd95 2 KbYf6AFFa+Rq1osNQPV85gn26Pk Fig.1a,d,e,f, S3; 3D quantification of DAPI stained DNA, G2 arrested cells by cdc2-asM17, Shortened the distance between genomic loci ------- COMMENT: ba8221c44a2afd95 3 E5P6mY6aABGIt0crxwqlgg/0d8U Fig.S2; G2 arrested cells by cdc2-asM17 ------- COMMENT: ba8221c44a2afd95 4 E8Vb31E27SHqijDsEtb3Sjro5Sw Fig.2,3, S4; Hi-C, G2 arrested cells by cdc2-asM17 ------- COMMENT: ba8221c44a2afd95 5 L7Uj0YKM45WgPtOKp7xE/8Ds9IY Fig.4, S5; G2 arrested cells by cdc2-asM17 ------- COMMENT: ba8221c44a2afd95 7 Wxi79MvW3VVDYEtTcccwE8HzEvU Fig.6e; 3D quantification of DAPI stained DNA, G2 arrested cells by cdc2-asM17, + Thiolutin ------- COMMENT: ba8221c44a2afd95 9 c5Q7/sVh2Nw0ruTrl9EkRHNaGXw Fig. 6b-d; Rad52 foci quantification, G2 arrested cells by cdc2-asM17 ------- COMMENT: ba8221c44a2afd95 11 MLEZbI3GohLIbRZnOtgOJ7IHSP0 Fig. 6b-d; Rad52 foci quantification, G2 arrested cells by cdc2-asM17, + Thiolutin ------- COMMENT: ba95f673c6553975 2 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: ba95f673c6553975 3 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: ba95f673c6553975 4 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: ba95f673c6553975 5 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: ba95f673c6553975 6 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: ba95f673c6553975 7 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: ba95f673c6553975 8 02l+3cqTh7UTFJSYjyKhzJabH68 fig1c ------- COMMENT: ba95f673c6553975 9 ouq88xK3qYy+YROPaqohtVfHs+0 fig 2g ------- COMMENT: ba95f673c6553975 10 LYYqsBxDhdti+L1KtaA72Ju+/es Cdc7p–GFP never disappeared from the old SPB (Sohrmann et al., 1998) and the type 1 nodes did not reform (Fig. 3B; supplementary material Movie 3) ------- COMMENT: ba95f673c6553975 11 v0itWquY/glePmsvpj6EN0+inl0 (Fig. 3C). ------- COMMENT: ba9d607d23dfef7d 1 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: ba9d607d23dfef7d 2 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: ba9d607d23dfef7d 3 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: bad7d44a253000ca 1 emQ2SXf5nAMPALcZR7wyvkHhtVk (comment: Pulse-field gel electrophoresis (PFGE), Polymerase chain reaction (PCR), Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 2 emQ2SXf5nAMPALcZR7wyvkHhtVk (comment: Pulse-field gel electrophoresis (PFGE), Polymerase chain reaction (PCR), Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 3 emQ2SXf5nAMPALcZR7wyvkHhtVk (comment: Pulse-field gel electrophoresis (PFGE), Polymerase chain reaction (PCR), Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 4 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 5 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 6 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 7 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 8 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 9 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 10 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 11 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 12 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 13 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 14 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 15 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 16 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 17 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 18 K09S6SSlU05H9Dxa97UCmckAq+A rpd1-S7A increased the rate of gross chromosomal rearrangement in the otherwise wild-type background. (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 19 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 20 +Xdty5VKcE0npWUvN8jeJqhXLxw rpd1-S7A reduced centromere noncoding RNA in the clr4∆ strain. (comment: Northern blot assay) ------- COMMENT: bad7d44a253000ca 21 IdOpIJMMZPjWyIv0d7k4X2MfZFw tfs1∆ reduced centromere noncoding RNA in the clr4∆ strain. (comment: Northern blot assay) ------- COMMENT: bad7d44a253000ca 22 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 23 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 24 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 25 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 26 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 27 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 28 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 29 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 30 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 31 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 32 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 33 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 34 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 35 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 36 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 37 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 38 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 39 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 40 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 41 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 42 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 43 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 44 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 45 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 46 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 47 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 48 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 49 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 106 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 122 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 123 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 124 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 125 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 126 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 127 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: bad7d44a253000ca 128 dfuOBMJA7LBhupghKMVdqxZ4MBo (comment: Monitoring an extra-chromosome ChL) ------- COMMENT: baddf4c6b54fd68c 1 o7euVaRhIhOHppegPqJfyYdzK2I mal3Δ cells exhibited lower microtubule growth speed throughout anaphase B Fig. 2G ------- COMMENT: baddf4c6b54fd68c 2 0kVH4dW3B4HL1voGSaDv5NoXKHE Fig. 2 - Figure supplement 1 klp5Δklp6Δ cells exhibited slightly longer microtubule growth events ------- COMMENT: baddf4c6b54fd68c 3 X+/nwTDAK82T5KgRaY8loGuMz2c Fig. 2 - Figure supplement 2F ------- COMMENT: baddf4c6b54fd68c 4 aIsYzeGShlb1Fe47hq2kGYr74xQ Fig. 2 - Figure supplement 2E ------- COMMENT: baddf4c6b54fd68c 5 P0MregibL1zCSDsH/CDnV0BIHwY Fig. 4 - figure supplement 2 We found that in both les1Δ and nem1Δ cells microtubule growth speed inside the nuclear bridge was faster than in wild-type cells ------- COMMENT: baddf4c6b54fd68c 6 P0MregibL1zCSDsH/CDnV0BIHwY Fig. 4 - figure supplement 2 We found that in both les1Δ and nem1Δ cells microtubule growth speed inside the nuclear bridge was faster than in wild-type cells ------- COMMENT: baddf4c6b54fd68c 7 5K8aaeTVfNJpBJ9uomwN/ps63kU Fig. 5E Ase1 is required for normal rescue distribution ------- COMMENT: baddf4c6b54fd68c 8 8Zwkkk1XcsUKq5tzK+DbjOC859U Fig. 5F, G The decrease in growth speed associated with internalisation of microtubules in the nuclear membrane bridge is reduced upon Ase1 deletion ------- COMMENT: baddf4c6b54fd68c 9 5z46vdigkMimOqZBHeTpA7B0P94 Fig. 5 supp 3E ------- COMMENT: baddf4c6b54fd68c 10 3UaAcdsMSe482RFPpMBa2BQwf+A (comment: Note: not sure about the term name and the child.) Fig. 3 supp 1A, C ------- COMMENT: baddf4c6b54fd68c 11 8Zwkkk1XcsUKq5tzK+DbjOC859U Fig. 5F, G The decrease in growth speed associated with internalisation of microtubules in the nuclear membrane bridge is reduced upon Ase1 deletion ------- COMMENT: bae1d88d8aeee742 1 bHJfiW1onsRQBHQdVhY8E0mOlbA n liquid YES medium at 27 ◦ C, the cells had a doubling time of ∼8 h, in contrast to 2 h 30 min for wild-type cells. ------- COMMENT: bae1d88d8aeee742 2 to9Mx4F0qxrdZYnXW6Oq0A+E9II While both wild- type and vps33􏰗 cells grew at 26 ◦ C, vps33􏰗 cells exhibited a temperature-sensitive growth at 37 ◦C (Figure 2A). ------- COMMENT: bae1d88d8aeee742 3 coBTmtFSwuxkBMiY8P2UDllndpE Fig. 2b ------- COMMENT: bae1d88d8aeee742 4 Ag6GoP3zsrPsg4IZ5Mmwasgbws4 Fig3 ------- COMMENT: bae1d88d8aeee742 5 S18KebUzgOypbM4vJbNb1v9zkOo strong sensitiv- ity to 100 mM CaCl2 (Figure 4A) ------- COMMENT: bae1d88d8aeee742 6 AIkUlFeaobi6Lk0oE22OqvjHOg8 (comment: at 5 μg/ml) ------- COMMENT: bae1d88d8aeee742 7 uVEmeMdSlDzJGGUX0Fg4dsJSu58 (comment: 4 mM) ------- COMMENT: bae1d88d8aeee742 8 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: bae1d88d8aeee742 9 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: bae1d88d8aeee742 10 +2XFMV1MQ4r+teF637sQosvJlkk fig 7a ------- COMMENT: bae1d88d8aeee742 11 rFkLIUhtkjpqxby29qQq52eDfz8 fig 7 (comment: CHECK prevacuolar compartment membrane) ------- COMMENT: bb24b96b242a33a1 1 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: bb24b96b242a33a1 2 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: bb24b96b242a33a1 3 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: bb24b96b242a33a1 4 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: bb24b96b242a33a1 5 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: bb24b96b242a33a1 6 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: bb24b96b242a33a1 7 VaXiSZZO7R52KMDhEepRtSmtYgA Fig.1 ------- COMMENT: bb24b96b242a33a1 8 VaXiSZZO7R52KMDhEepRtSmtYgA Fig.1 ------- COMMENT: bb24b96b242a33a1 9 VaXiSZZO7R52KMDhEepRtSmtYgA Fig.1 ------- COMMENT: bb24b96b242a33a1 10 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: bb24b96b242a33a1 11 VaXiSZZO7R52KMDhEepRtSmtYgA Fig.1 ------- COMMENT: bb24b96b242a33a1 12 VaXiSZZO7R52KMDhEepRtSmtYgA Fig.1 ------- COMMENT: bb24b96b242a33a1 13 Vy2juLAORqFhAL189p3r3DNPMEI Fig.2 ------- COMMENT: bb24b96b242a33a1 14 Vy2juLAORqFhAL189p3r3DNPMEI Fig.2 ------- COMMENT: bb24b96b242a33a1 15 Vy2juLAORqFhAL189p3r3DNPMEI Fig.2 ------- COMMENT: bb24b96b242a33a1 16 Vy2juLAORqFhAL189p3r3DNPMEI Fig.2 ------- COMMENT: bb24b96b242a33a1 17 Vy2juLAORqFhAL189p3r3DNPMEI Fig.2 ------- COMMENT: bb24b96b242a33a1 18 Vy2juLAORqFhAL189p3r3DNPMEI Fig.2 ------- COMMENT: bb24b96b242a33a1 19 Vy2juLAORqFhAL189p3r3DNPMEI Fig.2 ------- COMMENT: bb24b96b242a33a1 20 Vy2juLAORqFhAL189p3r3DNPMEI Fig.2 ------- COMMENT: bb24b96b242a33a1 21 Vy2juLAORqFhAL189p3r3DNPMEI Fig.2 ------- COMMENT: bb24b96b242a33a1 22 Vy2juLAORqFhAL189p3r3DNPMEI Fig.2 ------- COMMENT: bb24b96b242a33a1 23 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: bb24b96b242a33a1 24 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: bb24b96b242a33a1 25 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: bb24b96b242a33a1 26 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: bb24b96b242a33a1 27 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: bb24b96b242a33a1 28 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: bb24b96b242a33a1 29 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: bb24b96b242a33a1 30 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: bb24b96b242a33a1 31 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: bb24b96b242a33a1 32 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: bb24b96b242a33a1 33 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: bb24b96b242a33a1 34 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: bb24b96b242a33a1 35 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: bb24b96b242a33a1 36 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: bb24b96b242a33a1 37 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: bb24b96b242a33a1 38 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: bb24b96b242a33a1 39 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: bb24b96b242a33a1 40 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: bb24b96b242a33a1 41 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: bb24b96b242a33a1 43 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: bb24b96b242a33a1 44 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: bb24b96b242a33a1 45 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: bb24b96b242a33a1 46 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: bb24b96b242a33a1 47 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: bb24b96b242a33a1 48 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: bb24b96b242a33a1 49 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: bb24b96b242a33a1 50 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: bb24b96b242a33a1 51 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: bb24b96b242a33a1 52 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: bb24b96b242a33a1 53 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: bb24b96b242a33a1 54 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: bb24b96b242a33a1 55 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: bb24b96b242a33a1 56 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: bb24b96b242a33a1 57 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: bb24b96b242a33a1 58 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: bb24b96b242a33a1 59 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: bb24b96b242a33a1 60 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: bb24b96b242a33a1 61 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: bb24b96b242a33a1 62 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: bb24b96b242a33a1 63 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: bb24b96b242a33a1 64 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: bb24b96b242a33a1 65 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: bb24b96b242a33a1 66 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: bb24b96b242a33a1 67 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: bb24b96b242a33a1 68 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: bb24b96b242a33a1 69 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: bb24b96b242a33a1 70 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: bb24b96b242a33a1 71 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: bb24b96b242a33a1 72 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: bb24b96b242a33a1 73 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: bb24b96b242a33a1 74 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: bb24b96b242a33a1 75 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: bb24b96b242a33a1 76 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: bb24b96b242a33a1 77 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: bb24b96b242a33a1 78 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: bb24b96b242a33a1 79 bmFsn3TRgwK9n3xHdQbVAdUsiO4 (comment: CHECK negative regulation of extracellular phosphate aquisition) ------- COMMENT: bb24b96b242a33a1 80 bmFsn3TRgwK9n3xHdQbVAdUsiO4 (comment: CHECK negative regulation of extracellular phosphate aquisition) ------- COMMENT: bb24b96b242a33a1 81 VcFeTgVtJroGj+ZeRRwirW6qta4 (comment: CHECK negative extracellular phosphate aquisition (S5P,P6?,S7P)) ------- COMMENT: bb24b96b242a33a1 82 VJytQgrre+css5G2g3NIncxlLOA (comment: CHECK negative extracellular phosphate aquisition) ------- COMMENT: bb24b96b242a33a1 83 VcFeTgVtJroGj+ZeRRwirW6qta4 (comment: CHECK negative extracellular phosphate aquisition (S5P,P6?,S7P)) ------- COMMENT: bb51c9f75714cc60 1 S3LzyHX+xa65qnedo3gtJRpIWt8 (comment: CHECK conditional synthetic lethal with rna14-11) ------- COMMENT: bb51c9f75714cc60 2 bSJvd/mCewIZz6auh6EwulcEj+c (comment: splicing of rad21, nda3 and mad2 is also affected) ------- COMMENT: bb53d6c6ffb1be29 1 m9Z1/KKdgo7u7L+4LFTq3UrJuIY (comment: mah: localization to DSB sites also contributes to inference) ------- COMMENT: bb53d6c6ffb1be29 22 zQOWgxaK6+t84h77FoUHUfdAftU (comment mah: assayed substrate: exogenous histone H1) ------- COMMENT: bb53d6c6ffb1be29 23 Mn0McvG+Dg4TyAlmfWIXXpfReLQ (comment: CHECK mah: residue=T215) ------- COMMENT: bb53d6c6ffb1be29 26 uhkyMgqRu+/WL7bWgbnygOHayoI (comment: mah: same as rad51delta alone) ------- COMMENT: bb53d6c6ffb1be29 27 uhkyMgqRu+/WL7bWgbnygOHayoI (comment: mah: same as rad51delta alone) ------- COMMENT: bb53d6c6ffb1be29 29 uhkyMgqRu+/WL7bWgbnygOHayoI (comment: mah: same as rad51delta alone) ------- COMMENT: bb53d6c6ffb1be29 31 uhkyMgqRu+/WL7bWgbnygOHayoI (comment: mah: same as rad51delta alone) ------- COMMENT: bb53d6c6ffb1be29 35 uhkyMgqRu+/WL7bWgbnygOHayoI (comment: mah: same as rad51delta alone) ------- COMMENT: bb53d6c6ffb1be29 36 uhkyMgqRu+/WL7bWgbnygOHayoI (comment: mah: same as rad51delta alone) ------- COMMENT: bb53d6c6ffb1be29 39 MeYHMEbwZsLw/WuDeg2LeDBA0Lg (comment: mah: same as rqh1delta alone) ------- COMMENT: bb53d6c6ffb1be29 41 RliGfeD049LmyclcGcnvmIOWKNg (comment: mah: sensitivity depends on how highly overexpressed top3+ is; more top3+ -> lower sensitivity) ------- COMMENT: bb53d6c6ffb1be29 51 uhkyMgqRu+/WL7bWgbnygOHayoI (comment: mah: same as rad51delta alone) ------- COMMENT: bb53d6c6ffb1be29 52 uhkyMgqRu+/WL7bWgbnygOHayoI (comment: mah: same as rad51delta alone) ------- COMMENT: bb53d6c6ffb1be29 57 2S4jskGWzYju/FLwMQX+u+JTpwg (comment: mah: slighly more severe than rad50delta alone) ------- COMMENT: bb591d5e45b99277 3 I3LJCbkF7PQ/QAYBeaq81kV4iCM (comment: CHECK partial rescuie) ------- COMMENT: bb6fc09fed8a5c2c 1 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 2 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 3 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 4 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 5 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 6 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 7 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 8 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 9 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 10 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 11 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 12 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 13 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 14 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 15 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 16 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 17 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 18 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 19 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 20 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 21 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 22 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 23 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 24 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 25 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 26 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 27 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb6fc09fed8a5c2c 28 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: bb94978e6c7b86d7 3 SC7eygu+2TyBWTt61B2lG53tPfc Supplemental Figure S2 ------- COMMENT: bb94978e6c7b86d7 4 SC7eygu+2TyBWTt61B2lG53tPfc Supplemental Figure S2 ------- COMMENT: bb94978e6c7b86d7 7 N6gdfNLkuPfyqKNbJqqQxYyAr9g Fig. S3 ------- COMMENT: bb94978e6c7b86d7 8 ToYDxwHNxyqyAjWf4XV2uHueMUI Fig. 3E ------- COMMENT: bb94978e6c7b86d7 9 ToYDxwHNxyqyAjWf4XV2uHueMUI Fig. 3E ------- COMMENT: bb94978e6c7b86d7 10 ToYDxwHNxyqyAjWf4XV2uHueMUI Fig. 3E ------- COMMENT: bb94978e6c7b86d7 11 ToYDxwHNxyqyAjWf4XV2uHueMUI Fig. 3E ------- COMMENT: bb94978e6c7b86d7 12 b6IAA5jq+UeIwjjz7zZu5qhGt80 Fig. 4b ------- COMMENT: bbaa96d60dc05b65 1 Cy0SbLA2JxIzmewllptqyJNNJKU (comment: heterologous cytc as acceptor. they had to include this as etp1 wouldn't accept an electron otherwise.) "S. pombe does not express any endogenous mitochondrial cytochromes P450 that could act as terminal electron acceptors" ------- COMMENT: bbbf139e374eabbc 2 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: bbbf139e374eabbc 3 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: bbbf139e374eabbc 5 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: bbbf139e374eabbc 6 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: bbbf139e374eabbc 7 cQ08BfXoJu3sUREw3k32CA52dyc Fig. 5a ------- COMMENT: bbbf139e374eabbc 8 D2MnFM/0xeBrHD02sWpK8TyenhM fig5D ------- COMMENT: bbcfc3c19fa85d53 2 keOF2M9ZAz2eOSv5OjXgC6Bm/ZM (comment: localization independent of actin cytoskeleton (assayed using latrunculin A) and microtubule cytoskeleton (assayed using carbendazim)) ------- COMMENT: bbe966c7c31d9042 1 xB7IAlx0rxTk4FhEImNfEVVze7k (comment: CHECK Phosphorylates cdr2 at S755 in vitro) ------- COMMENT: bbe966c7c31d9042 2 GjZf2VIy7i2mnO19ybnHpt+dpNU Cdr2 phosphorylated by Pom1 at the CTD negatively regulates its activity ------- COMMENT: bbe966c7c31d9042 3 dR96Cmg+Mgdew34JcWZcS6oujwE Negatively regulated by Pom1 via phosphorylation of C-ter ------- COMMENT: bbe966c7c31d9042 9 k+6d90VnJwNskoE0dyK6V8b3ygc (comment: CONDITION low concentration (<0.25 uM) 3MB-PP1) ------- COMMENT: bbe966c7c31d9042 12 k+6d90VnJwNskoE0dyK6V8b3ygc (comment: CONDITION low concentration (<0.25 uM) 3MB-PP1) ------- COMMENT: bbe966c7c31d9042 14 nlOpdDrENS19Dy7H19O4cTQMZ2g (comment: CONDITION high concentration (1 uM) 3MB-PP1) ------- COMMENT: bbe966c7c31d9042 17 k+6d90VnJwNskoE0dyK6V8b3ygc (comment: CONDITION low concentration (<0.25 uM) 3MB-PP1) ------- COMMENT: bbe966c7c31d9042 28 k+6d90VnJwNskoE0dyK6V8b3ygc (comment: CONDITION low concentration (<0.25 uM) 3MB-PP1) ------- COMMENT: bbe966c7c31d9042 32 nlOpdDrENS19Dy7H19O4cTQMZ2g (comment: CONDITION high concentration (1 uM) 3MB-PP1) ------- COMMENT: bbe966c7c31d9042 34 nlOpdDrENS19Dy7H19O4cTQMZ2g (comment: CONDITION high concentration (1 uM) 3MB-PP1) ------- COMMENT: bbe966c7c31d9042 36 nlOpdDrENS19Dy7H19O4cTQMZ2g (comment: CONDITION high concentration (1 uM) 3MB-PP1) ------- COMMENT: bbe966c7c31d9042 47 KPclMANEX82Lx//Cv+58DNV4O50 (comment: high overexpression) ------- COMMENT: bbe966c7c31d9042 49 sX3tPQklufbD6XHStLgbj2k/uI8 (comment: moderate overexpression) ------- COMMENT: bbe966c7c31d9042 57 Cl7nksfhp7vmCrXVIu7J3PB1jR0 Pom1-as1 protein may preferentially localize to non-growing end. ------- COMMENT: bbe966c7c31d9042 75 W1FrAaQ9TruZnOa2k27h/AcurPc (comment: same as cdr2-S755A-758A alone) ------- COMMENT: bbe966c7c31d9042 76 W1FrAaQ9TruZnOa2k27h/AcurPc (comment: same as cdr2-S755A-758A alone) ------- COMMENT: bc2d8e5591c96dcc 4 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: bc2d8e5591c96dcc 5 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: bc37079d00ce0453 15 BcMypYvwaLSjaZS/r7aYmOXmwYw figure2 ------- COMMENT: bc37079d00ce0453 16 BcMypYvwaLSjaZS/r7aYmOXmwYw figure2 ------- COMMENT: bc37079d00ce0453 17 BcMypYvwaLSjaZS/r7aYmOXmwYw figure2 ------- COMMENT: bc37079d00ce0453 20 BcMypYvwaLSjaZS/r7aYmOXmwYw figure2 ------- COMMENT: bc37079d00ce0453 21 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: bc37079d00ce0453 22 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: bc37079d00ce0453 23 BcMypYvwaLSjaZS/r7aYmOXmwYw figure2 ------- COMMENT: bc37079d00ce0453 25 BcMypYvwaLSjaZS/r7aYmOXmwYw figure2 ------- COMMENT: bc37079d00ce0453 26 BcMypYvwaLSjaZS/r7aYmOXmwYw figure2 ------- COMMENT: bc37079d00ce0453 27 BcMypYvwaLSjaZS/r7aYmOXmwYw figure2 ------- COMMENT: bc37079d00ce0453 28 BcMypYvwaLSjaZS/r7aYmOXmwYw figure2 ------- COMMENT: bc37079d00ce0453 29 BcMypYvwaLSjaZS/r7aYmOXmwYw figure2 ------- COMMENT: bc6337774fa106c2 1 5DWqo03puFeQtodWqcCbmJ6nfyM fig 1A ------- COMMENT: bc6337774fa106c2 2 5DWqo03puFeQtodWqcCbmJ6nfyM fig 1A ------- COMMENT: bc6337774fa106c2 3 5DWqo03puFeQtodWqcCbmJ6nfyM fig 1A ------- COMMENT: bc6337774fa106c2 4 YQMxES+jTwxENiVUCWM+1qpcW9M (comment: CHECK in vitro) (Figure 1C, 2D) ------- COMMENT: bc6337774fa106c2 9 MiDzzjS0WpfK9zuuN7C5RYzbG1I (Figure 1D, Supplemental Figure 2A) ------- COMMENT: bc6337774fa106c2 10 MiDzzjS0WpfK9zuuN7C5RYzbG1I (Figure 1D, Supplemental Figure 2A) ------- COMMENT: bc6337774fa106c2 11 MiDzzjS0WpfK9zuuN7C5RYzbG1I (Figure 1D, Supplemental Figure 2A) ------- COMMENT: bc6337774fa106c2 12 MiDzzjS0WpfK9zuuN7C5RYzbG1I (Figure 1D, Supplemental Figure 2A) ------- COMMENT: bc6337774fa106c2 14 +wokP5OPjk/WHHU8WY/VWbPCcPA (Supplemental Figure 2B) ------- COMMENT: bc6337774fa106c2 15 +wokP5OPjk/WHHU8WY/VWbPCcPA (Supplemental Figure 2B) ------- COMMENT: bc6337774fa106c2 16 +wokP5OPjk/WHHU8WY/VWbPCcPA (Supplemental Figure 2B) ------- COMMENT: bc6337774fa106c2 17 +wokP5OPjk/WHHU8WY/VWbPCcPA (Supplemental Figure 2B) ------- COMMENT: bc6337774fa106c2 18 +wokP5OPjk/WHHU8WY/VWbPCcPA (Supplemental Figure 2B) ------- COMMENT: bc6337774fa106c2 19 tWNAsg1PjbtOhw/lHv23aKQf6gw fig 2b, c ------- COMMENT: bc6337774fa106c2 20 aKSuNP01YyCAxQf7IbiB9gen1ZA fig 2b ------- COMMENT: bc6337774fa106c2 23 6A/izyMcvtTS1Od7aZo0XYdPTr8 (comment: CHECK ABOLISHED) Fig 2C ------- COMMENT: bc6337774fa106c2 24 W/Ldc+kHVU6FHYXdO+TxDwSWUiw fig 3A. bub3 Δklp5 double mutants arrest as inviable micro-colonies of cells ------- COMMENT: bc6337774fa106c2 25 tFn9+VIfbWfRDBfDI1Pi6MrO5Mo fig 3a. ------- COMMENT: bc6337774fa106c2 26 YBFBut5qi9nGLEjnRFEx94qv4ks fig 3b ------- COMMENT: bc6337774fa106c2 27 YBFBut5qi9nGLEjnRFEx94qv4ks fig 3b ------- COMMENT: bc6337774fa106c2 28 YBFBut5qi9nGLEjnRFEx94qv4ks fig 3b ------- COMMENT: bc6337774fa106c2 31 MONXOONnktZw1XN6phJqbk/dkgA Fig 4c (comment: NORMAL SILENCING) ------- COMMENT: bc6337774fa106c2 32 eAyZquWnrvCI+Z1NXLrsnc3q72c Fig 4d (comment: NORMAL SILENCING) ------- COMMENT: bc6337774fa106c2 33 eAyZquWnrvCI+Z1NXLrsnc3q72c Fig 4d (comment: NORMAL SILENCING) ------- COMMENT: bc6337774fa106c2 34 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: bc6337774fa106c2 35 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: bc6337774fa106c2 36 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: bc6337774fa106c2 37 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: bc6337774fa106c2 38 AoYVDah5xnfd/EKswBj79v6F1Kg (Figure 4E). ------- COMMENT: bc6337774fa106c2 39 AoYVDah5xnfd/EKswBj79v6F1Kg (Figure 4E). ------- COMMENT: bc6337774fa106c2 42 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: bc6337774fa106c2 43 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: bc974ef58d58c8aa 1 KgMAJXxawh1PMdTYSHKzeQ5wshI (comment: vw: changed to match previous session terms RNA ...from cuf1􏰀 mutant spores showed loss of copper starvation-dependent induction of ctr4􏰁 and ctr5􏰁 gene expression, indicating that the copper-dependent reg ulation of ctr4􏰁 and ctr5􏰁 mRNAs required Cuf1 during germination and outgrowth.) ------- COMMENT: bc974ef58d58c8aa 2 0q656t8x8X5vmwCOArRTsDbIx90 (comment: vw: changed to match previous session terms RNA ...from cuf1􏰀 mutant spores showed loss of copper starvation-dependent induction of ctr4􏰁 and ctr5􏰁 gene expression, indicating that the copper-dependent reg ulation of ctr4􏰁 and ctr5􏰁 mRNAs required Cuf1 during germination and outgrowth.) ------- COMMENT: bc974ef58d58c8aa 3 0q656t8x8X5vmwCOArRTsDbIx90 (comment: vw: changed to match previous session terms RNA ...from cuf1􏰀 mutant spores showed loss of copper starvation-dependent induction of ctr4􏰁 and ctr5􏰁 gene expression, indicating that the copper-dependent reg ulation of ctr4􏰁 and ctr5􏰁 mRNAs required Cuf1 during germination and outgrowth.) ------- COMMENT: bc974ef58d58c8aa 6 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: bc974ef58d58c8aa 9 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: bc974ef58d58c8aa 13 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: bc974ef58d58c8aa 19 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: bc974ef58d58c8aa 21 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: bc974ef58d58c8aa 22 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: bc974ef58d58c8aa 23 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: bc974ef58d58c8aa 24 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: bc974ef58d58c8aa 25 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: bcaeb3349b4e8973 2 CpLPgm8i0a3op3IoshdhYxoBsbU fig 2c ------- COMMENT: bcaeb3349b4e8973 12 m6lgZGdjTCpJs75i9ImEc5PQJwU fig3B ------- COMMENT: bcb5a5b99a75c27a 3 RCFp2TKdrNhw2NCkUMRXc3t5KQ8 Nuclear Tos4 accumulation in cdc22-M45 decreased by cds1 deletion ------- COMMENT: bcb5a5b99a75c27a 4 W8JLHt0xQdCGaK/7WlFLbYiZTuU Nuclear Tos4 accumulation in the presence of HU at restrictive temperature in mcm4-M68-ts is reduced by cds1 deletion ------- COMMENT: bcb5a5b99a75c27a 5 SyDXowvipouiGL0NWu427HHoySk Lack of nuclear Tos4 accumulation in the presence of HU at restrictive temperature in mcm4-M68-ts-degron is reversed by chk1 deletion ------- COMMENT: bcb5a5b99a75c27a 8 QzHJC0v5dShFwP3g5eDR4NOoyQg (comment: temperature restrictive for cdc22-M45) ------- COMMENT: bcb5a5b99a75c27a 9 qbyWC64Hth7RPLvBcLGObnkYP+A (comment: temperature permissive for mcm4/cdc21-M68) ------- COMMENT: bcce385e477241ad 4 8cBKe2DefxLfaIG9dbSALYYFy1E (comment: assayed Cdc20 recruitment) ------- COMMENT: bcd1635bd0c34c02 29 302cXnV0YkVzloTA+nc2sJCX+O0 Among the four predicted genes for pyruvate decarboxylase, only the Phx1-dependent genes (pdc201+ and pdc202+) contributed to long-term survival as judged by mutation and overexpression studies. These findings indicate that the Phx1-mediated long-term survival is achieved primarily through increasing the synthesis and activity of pyruvate decarboxylase. Consistent with this hypothesis, we observed that Phx1 curtailed respiration when cells entered stationary phase. ------- COMMENT: bcd1635bd0c34c02 30 302cXnV0YkVzloTA+nc2sJCX+O0 Among the four predicted genes for pyruvate decarboxylase, only the Phx1-dependent genes (pdc201+ and pdc202+) contributed to long-term survival as judged by mutation and overexpression studies. These findings indicate that the Phx1-mediated long-term survival is achieved primarily through increasing the synthesis and activity of pyruvate decarboxylase. Consistent with this hypothesis, we observed that Phx1 curtailed respiration when cells entered stationary phase. ------- COMMENT: bcf6fb6825f4c05f 1 FlqhomaBqvUxnSalb+SyNIlcUaI (comment: CHECK pcn1-K107R and cdc17-K42) ------- COMMENT: bcf6fb6825f4c05f 17 Ox+C0vBHeQERlvwZDGD9JBK2A1w (comment: CHECK partial suppression of increased GCR) ------- COMMENT: bcf6fb6825f4c05f 18 Ox+C0vBHeQERlvwZDGD9JBK2A1w (comment: CHECK partial suppression of increased GCR) ------- COMMENT: bcf6fb6825f4c05f 19 Ox+C0vBHeQERlvwZDGD9JBK2A1w (comment: CHECK partial suppression of increased GCR) ------- COMMENT: bcf6fb6825f4c05f 20 +91pc0x2tKz+sBxKRRnZ+Rg4uiQ (comment: CHECK partial suppression of increased GCR; pcn1-K107R) ------- COMMENT: bcf6fb6825f4c05f 21 Ox+C0vBHeQERlvwZDGD9JBK2A1w (comment: CHECK partial suppression of increased GCR) ------- COMMENT: bcf6fb6825f4c05f 22 Ox+C0vBHeQERlvwZDGD9JBK2A1w (comment: CHECK partial suppression of increased GCR) ------- COMMENT: bcf6fb6825f4c05f 23 Ox+C0vBHeQERlvwZDGD9JBK2A1w (comment: CHECK partial suppression of increased GCR) ------- COMMENT: bcf6fb6825f4c05f 77 9NTDzlPVzQbtrYEqJNRcGVb9tCM (comment: worse than rad51delta alone) ------- COMMENT: bcf6fb6825f4c05f 78 9NTDzlPVzQbtrYEqJNRcGVb9tCM (comment: worse than rad51delta alone) ------- COMMENT: bcf6fb6825f4c05f 81 SHobzY4RmiEZjW/Nr9B35ExmNso (comment: CHECK Increased hydroxyurea sensitivity: pcn1-K164R) ------- COMMENT: bcf6fb6825f4c05f 82 0efk19MPdhemP8nC0Ke7MzS/N0w (comment: CHECK Increased methyl methanesulfonate sensitivity: pcn1-K164R) ------- COMMENT: bcf6fb6825f4c05f 83 Wal3Xf9XP6iRyZhbJCnHZD4cybE (comment: CHECK Increased camptothecin sensitivity: pcn1-K164R) ------- COMMENT: bcf6fb6825f4c05f 84 hoK1ZDHHj30OSORawMChBW7NW3o (comment: CHECK Decreased gene conversion: rad52-R45K) ------- COMMENT: bd11a19343ab24bb 1 qBRVVFiBu2RWKsc0skxiKLXrGVI The novel finding was that the recombinant S. pombe RNA triphosphatase by itself bound specifically to CTD peptides phosphorylated on Ser-5 and not to the unphosphorylated pep- tide or the Ser2-PO4 peptide (Fig. 3B). ------- COMMENT: bd11a19343ab24bb 2 vqcDUNR8vtRFydxKOP2stU4kbvw The key findings were that the S. pombe guany- lyltransferase bound equally well to the CTD Ser5-PO4 peptide and the Ser2-PO4/Ser5-PO4 peptide. Slightly less than one-fifth of the input protein was retained on the beads in both cases ------- COMMENT: bd11a19343ab24bb 3 5jeS4J7rm+0oeHE9gUKy/lM4TNY To gauge the role of the non-reiterated protein segment, we constructed an AD-Rpb1(1516 –1752) fusion clone and tested it in a directed two-hybrid assay paired with BD-Pce1 and BD- Pct1. We found that the Rpb1 interaction with both capping enzymes persisted when the AD fusion contained little more than the CTD repeats per se. ------- COMMENT: bd11a19343ab24bb 4 5jeS4J7rm+0oeHE9gUKy/lM4TNY To gauge the role of the non-reiterated protein segment, we constructed an AD-Rpb1(1516 –1752) fusion clone and tested it in a directed two-hybrid assay paired with BD-Pce1 and BD- Pct1. We found that the Rpb1 interaction with both capping enzymes persisted when the AD fusion contained little more than the CTD repeats per se. ------- COMMENT: bd11a19343ab24bb 5 vqcDUNR8vtRFydxKOP2stU4kbvw The key findings were that the S. pombe guany- lyltransferase bound equally well to the CTD Ser5-PO4 peptide and the Ser2-PO4/Ser5-PO4 peptide. Slightly less than one-fifth of the input protein was retained on the beads in both cases ------- COMMENT: bd4f213d7cad1349 20 lkID2PRMU5v8KTMAczpybzhi2IA (comment vw: sid2 phenotype indicates that Clp1 localization is independent of SIN) ------- COMMENT: bd5224d294af5974 1 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 2 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 3 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 4 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 5 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 6 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 7 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 8 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 9 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 10 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 11 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 12 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 13 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 14 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 15 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 16 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 17 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 18 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 19 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 20 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 21 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 22 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 23 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 24 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 25 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 26 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 27 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 28 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 29 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 30 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 31 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 32 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 33 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 34 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 35 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 36 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 37 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 38 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 39 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 40 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 41 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 42 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 43 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 44 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 45 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 46 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 47 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 48 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 49 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 50 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 51 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 52 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 53 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 54 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 55 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 56 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 57 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 58 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 59 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 60 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 61 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 62 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 63 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 64 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 65 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 66 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 67 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 68 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 69 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 70 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 71 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 72 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 73 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 74 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 75 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 76 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 77 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 78 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 79 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 80 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 81 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 82 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 83 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 84 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 85 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 86 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 87 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 88 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 89 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 90 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 91 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 92 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 93 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 94 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 95 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 96 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 97 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 98 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 99 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 100 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 101 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 102 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 103 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 104 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 105 dEhNLsBYYyGbAn5pxwnsZbXsWmk Table S2 ------- COMMENT: bd5224d294af5974 106 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 107 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 108 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 109 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 110 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 111 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 112 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 113 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 114 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 115 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 116 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 117 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 118 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 119 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 120 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 121 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 122 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 123 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 124 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 125 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 126 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 127 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 128 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 129 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 130 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 131 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 132 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 133 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 134 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 135 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 136 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 137 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 138 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 139 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 140 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 141 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 142 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 143 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 144 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 145 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 146 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 147 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 148 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 149 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 150 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 151 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 152 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 153 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 154 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 155 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 156 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 157 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 158 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 159 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 160 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 161 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 162 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 163 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 164 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 165 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 166 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 167 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 168 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 169 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 170 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 171 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 172 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 173 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 174 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 175 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 176 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 177 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 178 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 179 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 180 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 181 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 182 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 183 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 184 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 185 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 186 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 187 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 188 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 189 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 190 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 191 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 192 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 193 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 194 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 195 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 196 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 197 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 198 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 199 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 200 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 201 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 202 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 203 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 204 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 205 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 206 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 207 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 208 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 209 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 210 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 211 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 212 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 213 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 214 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 215 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 216 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 217 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 218 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 219 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 220 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 221 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 222 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 223 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 224 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 225 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 226 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 227 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 228 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 229 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: bd5224d294af5974 230 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 231 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 232 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 233 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 234 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 235 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 236 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 237 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 238 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 239 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 240 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 241 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 242 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 243 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 244 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 245 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 246 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 247 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 248 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 249 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 250 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 251 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 252 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 253 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 254 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 255 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 256 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 257 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 258 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 259 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 260 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 261 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 262 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 263 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 264 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 265 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 266 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 267 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 268 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd5224d294af5974 269 QEjf9Ay0RlMTbg0W2g7Yl2euhwk In summary, our observations suggest that the factors, Clr4, Sir2, Swd1 and Clr3 probably work in the same pathway as Swi6, but Brl2, Pof3, Cbp1 and Swi2 have an effect on donor selection through Swi6-dependent and -independent mechanisms. ------- COMMENT: bd5224d294af5974 271 QEjf9Ay0RlMTbg0W2g7Yl2euhwk In summary, our observations suggest that the factors, Clr4, Sir2, Swd1 and Clr3 probably work in the same pathway as Swi6, but Brl2, Pof3, Cbp1 and Swi2 have an effect on donor selection through Swi6-dependent and -independent mechanisms. ------- COMMENT: bd5224d294af5974 272 QEjf9Ay0RlMTbg0W2g7Yl2euhwk In summary, our observations suggest that the factors, Clr4, Sir2, Swd1 and Clr3 probably work in the same pathway as Swi6, but Brl2, Pof3, Cbp1 and Swi2 have an effect on donor selection through Swi6-dependent and -independent mechanisms. ------- COMMENT: bd5224d294af5974 273 QEjf9Ay0RlMTbg0W2g7Yl2euhwk In summary, our observations suggest that the factors, Clr4, Sir2, Swd1 and Clr3 probably work in the same pathway as Swi6, but Brl2, Pof3, Cbp1 and Swi2 have an effect on donor selection through Swi6-dependent and -independent mechanisms. ------- COMMENT: bd553a3d6f928456 3 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: bd553a3d6f928456 4 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: bd553a3d6f928456 5 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: bd553a3d6f928456 6 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: bd553a3d6f928456 7 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: bd553a3d6f928456 8 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: bd553a3d6f928456 9 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd553a3d6f928456 10 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: bd553a3d6f928456 11 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: bd553a3d6f928456 12 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: bd553a3d6f928456 13 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: bd553a3d6f928456 14 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: bd553a3d6f928456 16 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: bd553a3d6f928456 17 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: bdc3c1cc3b2e49bc 18 jPuhhksLIsrWZH44l5eDBVxlchM (comment: CHECK has substrates centromere outer repeat transcripts and polyA mRNA. Activated by mg2+) ------- COMMENT: bdc3c1cc3b2e49bc 47 5HHC6fRZVs8kPVydNUYwhWM7S3s (comment: does it produce 5' monoesters?) ------- COMMENT: bdc3c1cc3b2e49bc 62 jPuhhksLIsrWZH44l5eDBVxlchM (comment: CHECK has substrates centromere outer repeat transcripts and polyA mRNA. Activated by mg2+) ------- COMMENT: bdc65ab8c484c62c 28 Qprm5aED/HQOqH9Z2PyjJDfvnd8 (comment: deleted this extension because it refers to a pseudogene: annotation_extension=assayed_using(PomBase:SPBPB10D8.03) (mah 2014-08-05)) ------- COMMENT: bdeb75b22f017c39 11 XYOg1MyomEMLLWK/J7Rzc8TXBBs (comment: facs and author comment about growth) ------- COMMENT: be0d5c94b70a919d 32 QXuZ0SvDXzJixmWX+c7BUeaeZMs (comment: CHECK anaphase, elongating beyond cell end resulting in long curved spindle, requested) ------- COMMENT: be24c30941f300dc 10 M7Qk8zY7B3J+hfCNiG2z4+2SDbA (comment: CHECK might be abolished. Sometimes you see diploidization.) ------- COMMENT: be37fa1a04795ddd 10 mY0J9zH3rug4Fw0GZXgMDgV+5Uk (comment: CHECK mitotic G2/M transition delay) ------- COMMENT: be61b9ab28ac9d91 55 aiTkVkmo3P4/+KJQ2JCXmwDiSEo (comment: the evidence isn't great) ------- COMMENT: be9521cb5c4791ae 4 TBMsUvnlYcDpEmeGUAH2Kz1ylZ4 Fig 7 ------- COMMENT: be9521cb5c4791ae 7 Rtree0xXBUC973ZqIfnd6tJ72+Y Fig 6A ------- COMMENT: be9521cb5c4791ae 8 cWOc1PXhV78eewyU4+tR1ureLPw Fig 7A ------- COMMENT: be9521cb5c4791ae 9 kTvB5rbZANgdq/lfui571ZaBluI Fig 7B ------- COMMENT: be9521cb5c4791ae 10 4r6ubKddbE+s3bj0h3dUHnzrr7w Fig 7B ------- COMMENT: be9521cb5c4791ae 11 TrefqPMXPtEIj62lVYbjJ6unm9Q fig 7c ------- COMMENT: be9521cb5c4791ae 12 UoWggpjc06sdye9g7iqB+rK6O6c figure 8A ------- COMMENT: bea1c1e13005750d 54 G7SGDtxGtdl974CYnOaBB2hARFE (comment: generated from MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 55 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 56 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 57 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 58 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 59 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 60 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 61 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 62 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 63 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 64 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 65 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 66 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 67 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 68 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 69 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 70 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 71 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 72 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 73 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 74 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 75 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 76 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 77 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 78 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 79 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 80 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 81 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 82 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: bea1c1e13005750d 83 /vjTFt5IRhwUm8+FssXlh/ijmXw (comment: generated by MMS mutagenesis) ------- COMMENT: beeee61f81d10c76 52 +wefrxHUbbXt70TOksF8n/4cVa0 (comment: residue not determined, but probably Y173) ------- COMMENT: bf037075feb20f28 2 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: bf037075feb20f28 3 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: bf037075feb20f28 4 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: bf037075feb20f28 5 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: bf037075feb20f28 8 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: bf037075feb20f28 9 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: bf037075feb20f28 10 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: bf037075feb20f28 11 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: bf037075feb20f28 12 goF6M0CmHYnx3DoBPZFgnKrkbpE fig 4g ------- COMMENT: bf037075feb20f28 13 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: bf037075feb20f28 15 cRuP2/AQmNjmvB/ouXajr6w2GBc RNA transcript expression is increased during the stress response to zinc ions, but the increase is less than the transcript levels seen with full de-repression ------- COMMENT: bf037075feb20f28 17 cRuP2/AQmNjmvB/ouXajr6w2GBc RNA transcript expression is increased during the stress response to zinc ions, but the increase is less than the transcript levels seen with full de-repression ------- COMMENT: bf037075feb20f28 19 cRuP2/AQmNjmvB/ouXajr6w2GBc RNA transcript expression is increased during the stress response to zinc ions, but the increase is less than the transcript levels seen with full de-repression ------- COMMENT: bf037075feb20f28 23 T69qCmqL9YMxnMxMOpncERGxL4c Figure 7 - the goal of the experiments in this figure is to map the minimal region of loz1 that is required for zinc-responsiveness by generating gene fusion with MtfA, a transcription factor from Aspergillus nidulans that contains a double zinc finger domain with high similarity to Loz1 from S. pombe, but contains no other similarity. When expressed in S. pombe this gene fusion was regulated by zinc, suggesting that the region necessary for zinc responsiveness in S. pombe, maps to the zinc finger domains ------- COMMENT: bf037075feb20f28 26 9H3J50askm3wKNZg8HAs3FejPfM Figure 7 - the goal of the experiments in this figure is to map the minimal region of loz1 that is required for zinc-responsiveness by generating gene fusion with MtfA, a transcription factor from Aspergillus nidulans that contains a double zinc finger domain with high similarity to Loz1 from S. pombe, but contains no other similarity. When expressed in S. pombe this gene fusion was regulated by zinc, suggesting that the region necessary for zinc responsiveness in S. pombe, maps to the zinc finger domains and an upstream accessory domain ------- COMMENT: bf037075feb20f28 27 9H3J50askm3wKNZg8HAs3FejPfM Figure 7 - the goal of the experiments in this figure is to map the minimal region of loz1 that is required for zinc-responsiveness by generating gene fusion with MtfA, a transcription factor from Aspergillus nidulans that contains a double zinc finger domain with high similarity to Loz1 from S. pombe, but contains no other similarity. When expressed in S. pombe this gene fusion was regulated by zinc, suggesting that the region necessary for zinc responsiveness in S. pombe, maps to the zinc finger domains and an upstream accessory domain ------- COMMENT: bf8f7e547025faa5 1 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: bf8f7e547025faa5 2 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: bf8f7e547025faa5 3 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: bf8f7e547025faa5 4 YXHGFHU5LadekPlbrwHvPt7VWDU (Fig. 4) ------- COMMENT: bf8f7e547025faa5 5 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: bf8f7e547025faa5 6 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: bf8f7e547025faa5 7 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: bf8f7e547025faa5 8 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: bf8f7e547025faa5 9 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: bf8f7e547025faa5 10 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: bf9273ed8cb720b1 18 PRlJ7ORTZciV5oTtjSqzeX+sWdQ (comment: mixed population) ------- COMMENT: bf9273ed8cb720b1 20 PRlJ7ORTZciV5oTtjSqzeX+sWdQ (comment: mixed population) ------- COMMENT: bf9273ed8cb720b1 68 BELB54sH9HYL7NKsK5H+vfNohDQ (comment: temp semi-permissive for spp2-8 alone) ------- COMMENT: bf9273ed8cb720b1 69 BELB54sH9HYL7NKsK5H+vfNohDQ (comment: temp semi-permissive for spp2-8 alone) ------- COMMENT: bf9273ed8cb720b1 70 BELB54sH9HYL7NKsK5H+vfNohDQ (comment: temp semi-permissive for spp2-8 alone) ------- COMMENT: bf9273ed8cb720b1 71 90gwVBiHYmfOiY0+tJzJlSjOTaU (comment: higher temp, restrictive for spp2-8 alone) ------- COMMENT: bf9273ed8cb720b1 72 90gwVBiHYmfOiY0+tJzJlSjOTaU (comment: higher temp, restrictive for spp2-8 alone) ------- COMMENT: bf9273ed8cb720b1 73 90gwVBiHYmfOiY0+tJzJlSjOTaU (comment: higher temp, restrictive for spp2-8 alone) ------- COMMENT: bf9273ed8cb720b1 74 BELB54sH9HYL7NKsK5H+vfNohDQ (comment: temp semi-permissive for spp2-8 alone) ------- COMMENT: bf9273ed8cb720b1 75 BELB54sH9HYL7NKsK5H+vfNohDQ (comment: temp semi-permissive for spp2-8 alone) ------- COMMENT: bf9273ed8cb720b1 76 BELB54sH9HYL7NKsK5H+vfNohDQ (comment: temp semi-permissive for spp2-8 alone) ------- COMMENT: bf9273ed8cb720b1 77 BELB54sH9HYL7NKsK5H+vfNohDQ (comment: temp semi-permissive for spp2-8 alone) ------- COMMENT: bf9273ed8cb720b1 78 BELB54sH9HYL7NKsK5H+vfNohDQ (comment: temp semi-permissive for spp2-8 alone) ------- COMMENT: bf9273ed8cb720b1 79 J43D7U8+KZbCvvoOwE6bEkUNUUY (comment: temp semi-permissive for spp2-9 alone) ------- COMMENT: bf9273ed8cb720b1 80 J43D7U8+KZbCvvoOwE6bEkUNUUY (comment: temp semi-permissive for spp2-9 alone) ------- COMMENT: bf9273ed8cb720b1 81 J43D7U8+KZbCvvoOwE6bEkUNUUY (comment: temp semi-permissive for spp2-9 alone) ------- COMMENT: bf9273ed8cb720b1 82 TkBKCugRjiSVyUX/2QUnuzvrz3o (comment: higher temp, restrictive for spp2-9 alone) ------- COMMENT: bf9273ed8cb720b1 83 TkBKCugRjiSVyUX/2QUnuzvrz3o (comment: higher temp, restrictive for spp2-9 alone) ------- COMMENT: bf9273ed8cb720b1 84 TkBKCugRjiSVyUX/2QUnuzvrz3o (comment: higher temp, restrictive for spp2-9 alone) ------- COMMENT: bf9273ed8cb720b1 85 J43D7U8+KZbCvvoOwE6bEkUNUUY (comment: temp semi-permissive for spp2-9 alone) ------- COMMENT: bf9273ed8cb720b1 86 J43D7U8+KZbCvvoOwE6bEkUNUUY (comment: temp semi-permissive for spp2-9 alone) ------- COMMENT: bf9273ed8cb720b1 87 J43D7U8+KZbCvvoOwE6bEkUNUUY (comment: temp semi-permissive for spp2-9 alone) ------- COMMENT: bf9273ed8cb720b1 88 J43D7U8+KZbCvvoOwE6bEkUNUUY (comment: temp semi-permissive for spp2-9 alone) ------- COMMENT: bf9273ed8cb720b1 89 J43D7U8+KZbCvvoOwE6bEkUNUUY (comment: temp semi-permissive for spp2-9 alone) ------- COMMENT: bf9273ed8cb720b1 90 BELB54sH9HYL7NKsK5H+vfNohDQ (comment: temp semi-permissive for spp2-8 alone) ------- COMMENT: bf9273ed8cb720b1 91 J43D7U8+KZbCvvoOwE6bEkUNUUY (comment: temp semi-permissive for spp2-9 alone) ------- COMMENT: bf9cd1928a11ab87 1 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: bf9cd1928a11ab87 3 b7RolTWe60CWk8HgXsOF1ftkJuI (comment: CHECK Can we say somewhere - overexpresses genes involved by amino acid starvation, or something similar?) ------- COMMENT: bf9cd1928a11ab87 5 OIpmP4ey7H5bU/dai2a1L3vBeIg the expression of most genes induced by amino acid starvation in wild-type cells was not up-regulated, confirming that Gcn2 is the major mediator of this response ------- COMMENT: bf9cd1928a11ab87 6 BD5qWz1r4PwWVHBWHjIlAKQHm6k Ribosome profiling and matching RNA-seq in gcn2Δ cells treated or untreated with 3-AT revealed that the majority of the translationally induced genes did not respond to amino acid starvation (Fig. 2B) ------- COMMENT: bf9cd1928a11ab87 8 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: bf9cd1928a11ab87 9 TqhT9mNCZvGvLNKNORkhGW4CEXo Fig. 3B (comment: CHECK during normal growth) ------- COMMENT: bf9cd1928a11ab87 12 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: bf9cd1928a11ab87 16 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: bfa0f220c8549343 3 rKN2pICjZNw1r/9jL6+3vrepwR0 (comment: tyrosine; position(s) not determined) ------- COMMENT: bfa0f220c8549343 49 5L7/bA9Jutx0hULcOyh5e46y+Bc (comment: CHECK not sure this annotation is 100% supported, can revise later if needed.) ------- COMMENT: bfad67243d7e2d78 1 K1HbT1WD9y49ks2CyQ6lCu/xqik We incubated cell ghosts in the presence of ATP and the myosin-II ATPase inhibitor blebbistatin (0.1 mM; ref. 17). Whereas rings underwent rapid contraction in the absence of blebbistatin, ring contraction was abolished in the presence of blebbistatin (Fig. 3a, nD8). ------- COMMENT: bfb9017c2ff95ae7 18 JWjJArez8NdGC6fNHoOPdRKAdcI (comment: deleted existing genome maintence term, and annotated this instead, all things considered...) ------- COMMENT: bfcb5a1106855fcd 1 aSH1UoHehaOSoogIzECd67T4pn4 figure 1 ------- COMMENT: bfcb5a1106855fcd 2 aSH1UoHehaOSoogIzECd67T4pn4 figure 1 ------- COMMENT: bfcb5a1106855fcd 4 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: bfcb5a1106855fcd 5 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: bfcb5a1106855fcd 6 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: bfcb5a1106855fcd 7 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: bfcb5a1106855fcd 8 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: bfcb5a1106855fcd 9 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: bfcb5a1106855fcd 10 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: bfcb5a1106855fcd 11 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: bfcb5a1106855fcd 12 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: bfcb5a1106855fcd 13 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: bfcb5a1106855fcd 14 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: bfcb5a1106855fcd 15 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: bfcb5a1106855fcd 16 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: bfcb5a1106855fcd 17 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: bfcb5a1106855fcd 18 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: bfcb5a1106855fcd 21 eUkwlZ3xqzhyl6ZKYaD8oxmQdho figure 2c ------- COMMENT: bfcb5a1106855fcd 22 Er+JRCRz6pA1lzwVyPMjNKjmH9s figure 2d ------- COMMENT: bfcb5a1106855fcd 23 Er+JRCRz6pA1lzwVyPMjNKjmH9s figure 2d ------- COMMENT: bfcb5a1106855fcd 24 Er+JRCRz6pA1lzwVyPMjNKjmH9s figure 2d ------- COMMENT: bfcb5a1106855fcd 25 Er+JRCRz6pA1lzwVyPMjNKjmH9s figure 2d ------- COMMENT: bfcb5a1106855fcd 26 Er+JRCRz6pA1lzwVyPMjNKjmH9s figure 2d ------- COMMENT: bfcb5a1106855fcd 27 Er+JRCRz6pA1lzwVyPMjNKjmH9s figure 2d ------- COMMENT: bfcb5a1106855fcd 28 Er+JRCRz6pA1lzwVyPMjNKjmH9s figure 2d ------- COMMENT: bfcb5a1106855fcd 29 Er+JRCRz6pA1lzwVyPMjNKjmH9s figure 2d ------- COMMENT: bfcb5a1106855fcd 30 Er+JRCRz6pA1lzwVyPMjNKjmH9s figure 2d ------- COMMENT: bfcb5a1106855fcd 31 Er+JRCRz6pA1lzwVyPMjNKjmH9s figure 2d ------- COMMENT: bfcb5a1106855fcd 32 7ModPd7vVF8tNNhbiUu3iuCsnLo figure3A (comment: increased cacineurin signalling) ------- COMMENT: bfcb5a1106855fcd 33 INKjIJhiKd5d0GrRKxw2XwM62Jo figure3B ------- COMMENT: bfcb5a1106855fcd 34 HVVGW4LNmpL3jmUJT/NRQ6xM2ek figure3C ------- COMMENT: bfcb5a1106855fcd 35 x53fXfF1hiA2T8P90IhexA0desA figure3D ------- COMMENT: bfcb5a1106855fcd 36 3eKgIGRIinHeKjS90w9DsBSOiU4 FIG 4 (comment: Ccr1 is a molecular target of TAM.) ------- COMMENT: bfcb5a1106855fcd 38 aSH1UoHehaOSoogIzECd67T4pn4 figure 1 ------- COMMENT: bfcb5a1106855fcd 39 w0wKkAH6w7GsNsCMiOGd8acmq1c figure 2b ------- COMMENT: bfcb5a1106855fcd 40 w0wKkAH6w7GsNsCMiOGd8acmq1c figure 2b ------- COMMENT: bfcb5a1106855fcd 41 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: bfd24f1d8129fc47 2 znL/6NZZ68+xZAVo6TcFJBrwPOA Figure 1a, b found that Spc1 overexpression could partially rescue the ‘wee’ phenotype of Δwee1 cells ( ------- COMMENT: bfd24f1d8129fc47 3 EZs/EHiz3C4moY+5ttuiTILAUDA Figure 1d) These mutants have a ts allele of Cdc25 which becomes non‐functional at 37°C. Cdc2, therefore, remains inactive and the cells arrest at the G2/M boundary. Prolonged incubation at this temperature leads to cell death. We found that Spc1 overexpres- sion led to an increase in their loss of viability ( ------- COMMENT: bfd24f1d8129fc47 4 09XzWahrfHrAXrJDyuRoTcGIOCo Thus, although results from experiments in a Spc1 loss of function background support a role for Spc1 in promoting mitotic entry, gain of function experiments suggest a role for Spc1 in inhibition of mitotic entry. Therefore, while Δspc1 cells can serve as a good model to study its role in mitotic promotion, the overexpression model that we are working with will be a very good model to study its role in inhibition of mitosis. ------- COMMENT: bfd24f1d8129fc47 5 Ex0CaqkT/K+YAdSKLZPrdud/Os0 Fig. 2a The high level of Cdc25 activity in these cells causes premature mitotic entry and they divide at shorter cell lengths. ------- COMMENT: bfd24f1d8129fc47 6 P0xxzSGvAJiMP8JxSyWeJLfIinY (Figure 2a). We found that Spc1 OE could partially rescue the cell length shortening in these cells ------- COMMENT: bfd24f1d8129fc47 7 LeePdO8uTfLFH4JsFj1atjFWWS0 We found that the percentage of cells showing nuclear Cdc25 was reduced upon Spc1 overexpres- sion. Quantitative estimations showed a clear decrease in the nuclear/cytoplasmic ratio of Cdc25 in these cells (Figure 2c) ------- COMMENT: bfd24f1d8129fc47 8 vR+HrhKs2kr26PhWbiv5mFjJZHY Figure 2e, f We found that, unlike in otherwise wt cells, Spc1 over- expression could not rescue the short cell length phenotype of Δrad24 mutants overexpressing Cdc25 ------- COMMENT: bfd24f1d8129fc47 9 vR+HrhKs2kr26PhWbiv5mFjJZHY Figure 2e, f We found that, unlike in otherwise wt cells, Spc1 over- expression could not rescue the short cell length phenotype of Δrad24 mutants overexpressing Cdc25 ------- COMMENT: bfd24f1d8129fc47 10 aIgNBGqVOoNFgelhklgDSvx1LYA fig3a ------- COMMENT: bfd24f1d8129fc47 11 SlzRNHNdP+yIONV0za706tB7a7A (Figure 3a). Spc1 overexpression led to a clear increase in the cell length at septation of Δsrk1 cells overexpressing Cdc25 ------- COMMENT: bfd24f1d8129fc47 12 ky/+0122BdU7xvxUw6twS2LfsEw Figure 3a ------- COMMENT: bfd24f1d8129fc47 13 tNFDNFzcz37CgVpJryYhb1aCUyE (Figure 4a) We found that Spc1 overexpression led to an increase in Rad24 levels of the wt cells overexpressing Cdc25. ------- COMMENT: bfd24f1d8129fc47 14 ZXk/msKzgzWRwDEZhoh+fSeq8F0 (Figure 4b) We also observed that Spc1 overexpression caused an increase in Rad24 levels in Δwee1 cells ------- COMMENT: bfd24f1d8129fc47 15 U10QXloQ4o/Vi6NrXLmZrrboj1I we overexpressed Rad24 in wt and Δwee1 cells.Fig 4D Indeed we found that Rad24 overexpression could increase cell length at division in both the backgrounds ------- COMMENT: bfd24f1d8129fc47 16 U10QXloQ4o/Vi6NrXLmZrrboj1I we overexpressed Rad24 in wt and Δwee1 cells.Fig 4D Indeed we found that Rad24 overexpression could increase cell length at division in both the backgrounds ------- COMMENT: bfd24f1d8129fc47 17 33owwYqak7k4OnDwRNkWHkVfzVA (Figure 4c) Another interesting fact that was also revealed in our experiments was that the Δwee1 cells had considerably lower Rad24 mRNA levels compared with wt cells ------- COMMENT: bfd3f85ba1c4e658 5 brDQor4FOn7G+XQe5b430W3FXIE Increased Cdc22 oxidation attenuated by 2 mM Glutathione. Increased Cdc22 oxidation attenuated by deleting tpx1. ------- COMMENT: bfd3f85ba1c4e658 15 brDQor4FOn7G+XQe5b430W3FXIE Increased Cdc22 oxidation attenuated by 2 mM Glutathione. Increased Cdc22 oxidation attenuated by deleting tpx1. ------- COMMENT: bfd3f85ba1c4e658 16 UJWuWn+AUr0NeM9bhD9mEfskiIE (comment: CHECK 2 mM Glutathione restores aerobic growth.) ------- COMMENT: bfd3f85ba1c4e658 24 UJWuWn+AUr0NeM9bhD9mEfskiIE (comment: CHECK 2 mM Glutathione restores aerobic growth.) ------- COMMENT: bfd3f85ba1c4e658 26 RxZYBqNcJALANa9EGbo2ndT4QKw (comment: CHECK aerobic conditions) ------- COMMENT: bfd3f85ba1c4e658 48 RxZYBqNcJALANa9EGbo2ndT4QKw (comment: CHECK aerobic conditions) ------- COMMENT: bfd3f85ba1c4e658 49 RxZYBqNcJALANa9EGbo2ndT4QKw (comment: CHECK aerobic conditions) ------- COMMENT: bfd3f85ba1c4e658 57 RxZYBqNcJALANa9EGbo2ndT4QKw (comment: CHECK aerobic conditions) ------- COMMENT: bfd3f85ba1c4e658 58 RxZYBqNcJALANa9EGbo2ndT4QKw (comment: CHECK aerobic conditions) ------- COMMENT: bfd3f85ba1c4e658 59 RxZYBqNcJALANa9EGbo2ndT4QKw (comment: CHECK aerobic conditions) ------- COMMENT: bfd3f85ba1c4e658 60 RxZYBqNcJALANa9EGbo2ndT4QKw (comment: CHECK aerobic conditions) ------- COMMENT: bfdd2ebf8fdc9f81 1 1qqwjwA9XBs85ANZdX5L4XJNLN8 (comment: CONDITION 50 mM) ------- COMMENT: bfdd2ebf8fdc9f81 2 rORKoDuStyXkb4rmMqhhtVvjpqQ Fig. 1A. ------- COMMENT: bfdd2ebf8fdc9f81 3 ST79MW/VXwZ06UKIZumA/1lpkqA Fig. 1A. All cells lysed while undergoing division and the daughter cells remained attached to one another. ------- COMMENT: bfdd2ebf8fdc9f81 4 ST79MW/VXwZ06UKIZumA/1lpkqA Fig. 1A. All cells lysed while undergoing division and the daughter cells remained attached to one another. ------- COMMENT: bfdd2ebf8fdc9f81 6 cWZBzT+AHg0K/1vTgV04/7FZdTg with lysis (these are not chained cells) (Fig. 2C).....cells septum degradation appeared to initiate at a single position around the cell circumference and the entire cell wall disappeared from this area ------- COMMENT: bfdd2ebf8fdc9f81 7 l0qUEh/kddumErLP8w8Cx45Sx5Q (Fig. 3B). Gyp10 localized to structures reminiscent of the endoplasmic reticulum (Broughton et al., 1997) when expressed from the low strength nmt81 promoter ------- COMMENT: bfdd2ebf8fdc9f81 8 6YVNUZ7WhDv/EkdczeMW+sCrc0Y Fig. 3C. ------- COMMENT: bfdd2ebf8fdc9f81 9 v0itWquY/glePmsvpj6EN0+inl0 (Fig. 3C). ------- COMMENT: bfdd2ebf8fdc9f81 10 v0itWquY/glePmsvpj6EN0+inl0 (Fig. 3C). ------- COMMENT: bfdd2ebf8fdc9f81 11 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: bfdd2ebf8fdc9f81 12 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: bfdd2ebf8fdc9f81 13 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: bfdd2ebf8fdc9f81 14 eUPTP7xUtVbc6bYGfxt15gev0zY Consistent with a role in vesicular trafficking, gyp10  showed a strong negative genetic interaction with cells lacking the exocyst subunit, Exo70p (Wang et al., 2002) (Fig. 3C). ------- COMMENT: bfdd2ebf8fdc9f81 15 5WnqXNWd678OP3UFMZ7RJu+oU9E Both proteins localized to the division site(Fig. 4B,D). Rgf1p-GFP formed rings (Fig. 4B) late in mitosis as only cells containing segregated DNA masses contained them (data not shown and Fig. 7A) ------- COMMENT: bfdd2ebf8fdc9f81 16 4JPywZyv536MIvQ5D6NTIZVAYCQ .By contrast, Rgf3p-GFP was not detected at cell ends, only at the medial region of the cell (Fig. 4D). Furthermore, Rgf3p rings constricted (Fig. 4D, inset). These differences in pattern are illustrated in Fig. 4E ------- COMMENT: bfdd2ebf8fdc9f81 17 w63sFb/hzO2xC2k1JLs4DsAQefs Rgf1p-GFP was also detected at cell ends (Fig. 4B). ------- COMMENT: bfdd2ebf8fdc9f81 18 1jLg6aKXClk3gvx7tPWq4lkph8Y Figue 5C ------- COMMENT: bfdd2ebf8fdc9f81 19 1jLg6aKXClk3gvx7tPWq4lkph8Y Figue 5C ------- COMMENT: bfdd2ebf8fdc9f81 20 H4+lz3HQJPGZV5fTgAxB9ja/xQA Figue 5C Interestingly, overexpression of gpt10+ (Fig. 5G), but not rho1+ (data not shown) restored the localization of Rgf3 in lad1-1 cells at 36°C. ------- COMMENT: bfdd2ebf8fdc9f81 21 CVK6+3nm8sI0+0/mDMmghhZejTY (comment: rgf3+ is essential) ------- COMMENT: bfdd2ebf8fdc9f81 22 CVK6+3nm8sI0+0/mDMmghhZejTY (comment: rgf3+ is essential) ------- COMMENT: bfdd2ebf8fdc9f81 23 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: bfdd2ebf8fdc9f81 24 KBFiiRuZW0kfEBey2wnEgWC5SZk (comment: CHECK `SYNTHETIC LETHAL) ------- COMMENT: bfdd2ebf8fdc9f81 25 cigkxGmyXqZOu9byfu7bXS1/YCk asked whether Rho1p was able to localize correctly to the medial region of the cell in the lad1-1 strain that we had shown lacks medially placed Rgf3p (Fig. 5C). We found that it did (Fig. 6B). ------- COMMENT: bfdd2ebf8fdc9f81 26 R9YJ4sKRQygIHXOTcAx6Kl1ct0s Ace2p, we examined whether Rgf3p levels were altered in cells lacking or overproducing Ace2p. Overproduction of Ace2p led to increased Rgf3p levels whereas Rgf3p was less abundant in cells lacking Ace2p (Fig. 7B). However, Rgf3p-Myc13 was clearly detectable in the absence of Ace2p suggesting that other factors cooperate with Ace2p to regulate rgf3+ expression. ------- COMMENT: bfdd2ebf8fdc9f81 27 IuIOXPh6YC2TPJcD7UKdK6II/GM To determine whether Rgf3p production was controlled by Ace2p, we examined whether Rgf3p levels were altered in cells lacking or overproducing Ace2p. Overproduction of Ace2p led to increased Rgf3p levels whereas Rgf3p was less abundant in cells lacking Ace2p (Fig. 7B ------- COMMENT: bfdd2ebf8fdc9f81 28 IuIOXPh6YC2TPJcD7UKdK6II/GM To determine whether Rgf3p production was controlled by Ace2p, we examined whether Rgf3p levels were altered in cells lacking or overproducing Ace2p. Overproduction of Ace2p led to increased Rgf3p levels whereas Rgf3p was less abundant in cells lacking Ace2p (Fig. 7B ------- COMMENT: bfdd2ebf8fdc9f81 29 u7Eb4xJIe7uX2EUsP5EqF/HG/qc Rgf3p appears necessary to stimulate Rho1p-mediated activation of a glucan synthase crucial after septation for proper new cell-end formation. ------- COMMENT: bfe0472c4af56347 1 cnvWi50D2+qSssDDkaRDgd9fBOE fig 1A Defect in Checkpoint Signaling. ------- COMMENT: bfe0472c4af56347 2 mwkDVfaNZDogm/jQklPtKSU8XeI fig 1A ------- COMMENT: bfe0472c4af56347 3 mwkDVfaNZDogm/jQklPtKSU8XeI fig 1A ------- COMMENT: bfe0472c4af56347 4 mwkDVfaNZDogm/jQklPtKSU8XeI fig 1A ------- COMMENT: bfe0472c4af56347 5 mwkDVfaNZDogm/jQklPtKSU8XeI fig 1A ------- COMMENT: bfe0472c4af56347 6 mwkDVfaNZDogm/jQklPtKSU8XeI fig 1A ------- COMMENT: bfe0472c4af56347 7 mwkDVfaNZDogm/jQklPtKSU8XeI fig 1A ------- COMMENT: bfe0472c4af56347 8 mwkDVfaNZDogm/jQklPtKSU8XeI fig 1A ------- COMMENT: bfe0472c4af56347 9 mwkDVfaNZDogm/jQklPtKSU8XeI fig 1A ------- COMMENT: bfe0472c4af56347 10 N3IrWkN6FUtHfTt6JCaaWP4L+8c fig 2A ------- COMMENT: bfe0472c4af56347 11 N3IrWkN6FUtHfTt6JCaaWP4L+8c fig 2A ------- COMMENT: bfe0472c4af56347 12 N3IrWkN6FUtHfTt6JCaaWP4L+8c fig 2A ------- COMMENT: bfe0472c4af56347 13 zKxgyRAsYgWL7f58cuMZpRjtV+I fig 2B ------- COMMENT: bfe0472c4af56347 14 zKxgyRAsYgWL7f58cuMZpRjtV+I fig 2B ------- COMMENT: bfe0472c4af56347 15 zKxgyRAsYgWL7f58cuMZpRjtV+I fig 2B ------- COMMENT: bfe0472c4af56347 16 oAQJxN0m+ULu4EefQZ660auP/ug fig 2C ------- COMMENT: bfe0472c4af56347 17 oAQJxN0m+ULu4EefQZ660auP/ug fig 2C ------- COMMENT: bfe0472c4af56347 18 oAQJxN0m+ULu4EefQZ660auP/ug fig 2C ------- COMMENT: bfe0472c4af56347 19 oAQJxN0m+ULu4EefQZ660auP/ug fig 2C ------- COMMENT: bfe0472c4af56347 20 cWU1gxrBhSdpW25jqWFTdTZ9qBA fig 3A ------- COMMENT: bfe0472c4af56347 21 cWU1gxrBhSdpW25jqWFTdTZ9qBA fig 3A ------- COMMENT: bfe0472c4af56347 22 cWU1gxrBhSdpW25jqWFTdTZ9qBA fig 3A ------- COMMENT: bfe0472c4af56347 23 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: bfe0472c4af56347 24 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: bfe0472c4af56347 25 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: bfe0472c4af56347 26 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: bfe0472c4af56347 27 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: bfe0472c4af56347 28 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: bfe0472c4af56347 29 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: bfe0472c4af56347 32 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: bfe0472c4af56347 33 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: bfe0472c4af56347 34 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: bfe0472c4af56347 35 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: bfe0472c4af56347 36 tc3rJxZshAX+tmBq7BR9TkADO4o Fig 7A ------- COMMENT: bfe0472c4af56347 37 kTvB5rbZANgdq/lfui571ZaBluI Fig 7B ------- COMMENT: bfe0472c4af56347 38 kTvB5rbZANgdq/lfui571ZaBluI Fig 7B ------- COMMENT: bfe0472c4af56347 39 oAQJxN0m+ULu4EefQZ660auP/ug fig 2C ------- COMMENT: c03bdfb180e8b701 1 Rn7EroX/veYhO0/YXjuXSjNMz5Q (data not shown) ------- COMMENT: c03bdfb180e8b701 5 QGsIrdKYh0PwBVE+nvrH2Enokkg figure 1a ------- COMMENT: c03bdfb180e8b701 6 3GMCD/9ZUpUng4mAm2DVyReIgpI figure 1b ------- COMMENT: c03bdfb180e8b701 7 3GMCD/9ZUpUng4mAm2DVyReIgpI figure 1b ------- COMMENT: c03bdfb180e8b701 8 2o7AxAWDxM9U1zw+Mb3TxEC3uCY figure 1b (I) ------- COMMENT: c03bdfb180e8b701 9 2o7AxAWDxM9U1zw+Mb3TxEC3uCY figure 1b (I) ------- COMMENT: c03bdfb180e8b701 10 jC9NkjeBIyfM2zIhNwBg6ZdMh60 figure 1C ------- COMMENT: c03bdfb180e8b701 11 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: c03bdfb180e8b701 17 4m0JwatGnIW3dsxSoFsHomN2lXY indicated by decreased polysome to monosome ratio ------- COMMENT: c04525e422559462 2 2uXHsRQ73TXDbRuvHl10bMac5II (comment: not much evidence of specificity for H3 vs. H4 or position) ------- COMMENT: c04525e422559462 21 mpZiEYnlUaBySC4lGE0FH/UP6pM actually inferred from combination of phenotype and sequence similarity ------- COMMENT: c04525e422559462 27 TOqmkuMqENggA5gCqsYbzZwpyZ8 (comment: CHECK not (coincident_with(SO:0001789) | coincident_with(SO:0001795))) ------- COMMENT: c04525e422559462 31 mpZiEYnlUaBySC4lGE0FH/UP6pM actually inferred from combination of phenotype and sequence similarity ------- COMMENT: c04525e422559462 32 mpZiEYnlUaBySC4lGE0FH/UP6pM actually inferred from combination of phenotype and sequence similarity ------- COMMENT: c04525e422559462 33 mpZiEYnlUaBySC4lGE0FH/UP6pM actually inferred from combination of phenotype and sequence similarity ------- COMMENT: c04525e422559462 34 WQd6RHcVjJ8TGcQ5dI5o0dp3OW8 (comment: broad specificity;) actually inferred from combination of phenotype and sequence similarity ------- COMMENT: c04525e422559462 35 WQd6RHcVjJ8TGcQ5dI5o0dp3OW8 (comment: broad specificity;) actually inferred from combination of phenotype and sequence similarity ------- COMMENT: c04525e422559462 36 WQd6RHcVjJ8TGcQ5dI5o0dp3OW8 (comment: broad specificity;) actually inferred from combination of phenotype and sequence similarity ------- COMMENT: c04525e422559462 37 WQd6RHcVjJ8TGcQ5dI5o0dp3OW8 (comment: broad specificity;) actually inferred from combination of phenotype and sequence similarity ------- COMMENT: c04525e422559462 38 WQd6RHcVjJ8TGcQ5dI5o0dp3OW8 (comment: broad specificity;) actually inferred from combination of phenotype and sequence similarity ------- COMMENT: c04525e422559462 39 WQd6RHcVjJ8TGcQ5dI5o0dp3OW8 (comment: broad specificity;) actually inferred from combination of phenotype and sequence similarity ------- COMMENT: c04525e422559462 40 WQd6RHcVjJ8TGcQ5dI5o0dp3OW8 (comment: broad specificity;) actually inferred from combination of phenotype and sequence similarity ------- COMMENT: c04525e422559462 41 WQd6RHcVjJ8TGcQ5dI5o0dp3OW8 (comment: broad specificity;) actually inferred from combination of phenotype and sequence similarity ------- COMMENT: c04525e422559462 42 WQd6RHcVjJ8TGcQ5dI5o0dp3OW8 (comment: broad specificity;) actually inferred from combination of phenotype and sequence similarity ------- COMMENT: c061f382feb3bbd0 5 TMK+Y4mPoZ1Aace0o8o9q5eGKYg Figure 4G ------- COMMENT: c061f382feb3bbd0 23 bvE35lh1IuWvAeFdfP7Qzi1ZMzM figure 5E ------- COMMENT: c061f382feb3bbd0 25 Icz9GkaldwaD6ohboqw59qoLNhU Figure 7D ------- COMMENT: c061f382feb3bbd0 26 Icz9GkaldwaD6ohboqw59qoLNhU Figure 7D ------- COMMENT: c061f382feb3bbd0 30 CeHpqh3HedaTETzfnMvct9XQVyA indicated by increased mad2 on unattached kinetochores ------- COMMENT: c061f382feb3bbd0 31 hCv5p0zcw9wEk7lg46nEXfMZA4g Tracking of the inter-SPB distance indicated that the kinetics of spindle elongation in the kis1-1 mad2D double mutant was ameliorated compared with the kis1-1 single mutant (Figure S9). ------- COMMENT: c06e8b663aa9d810 1 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: c06e8b663aa9d810 2 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: c06e8b663aa9d810 3 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: c06e8b663aa9d810 4 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: c06e8b663aa9d810 5 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: c06e8b663aa9d810 6 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: c06e8b663aa9d810 7 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: c06e8b663aa9d810 8 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: c06e8b663aa9d810 9 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: c06e8b663aa9d810 10 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: c06e8b663aa9d810 11 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: c06e8b663aa9d810 12 Aa1cvCko0341swdUX4S1pPwquOk Figure 2B ------- COMMENT: c06e8b663aa9d810 18 vOrtZ7OuqI2gSJSMkZutnYt5zRM Figure 3A EXP says increased, but is normal compared to WT (i.e ura4 insertion derepresses) ------- COMMENT: c06e8b663aa9d810 19 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: c06e8b663aa9d810 20 QM+OIervucEHfDXV9bERdBnUu3s Figure 3C spreading is still within the central domain, to the flanking tRNAs ------- COMMENT: c06e8b663aa9d810 21 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: c06e8b663aa9d810 22 b+00gXuAJVmG6MoHxSub6s9WxVw Figure 4C ------- COMMENT: c06e8b663aa9d810 23 MH1gvSjAPqOM0wfzJ6P5nVe2fro Figure S8 ------- COMMENT: c0af69aa51ff9eff 4 /1CcnxN/NJcLlIsp2kbYdrweGxE figure 2a ( stretched chromaitn along elongating spindle at anaphase B) ------- COMMENT: c0af69aa51ff9eff 7 lndeXQ0ysjk7K/pYlUA6+k0iktE figure 2b ------- COMMENT: c0af69aa51ff9eff 8 zoIll54S0Jw1KoVp9rPOHkxLj0s figure 2b chromsome detached from spindle ------- COMMENT: c0af69aa51ff9eff 9 lndeXQ0ysjk7K/pYlUA6+k0iktE figure 2b ------- COMMENT: c0af69aa51ff9eff 10 lndeXQ0ysjk7K/pYlUA6+k0iktE figure 2b ------- COMMENT: c0af69aa51ff9eff 15 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: c0af69aa51ff9eff 16 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: c0af69aa51ff9eff 17 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: c0af69aa51ff9eff 18 uP2b450kx3y/u6z3D7bDUa+eEuM fig8 ------- COMMENT: c0af69aa51ff9eff 19 uP2b450kx3y/u6z3D7bDUa+eEuM fig8 ------- COMMENT: c0af69aa51ff9eff 20 uP2b450kx3y/u6z3D7bDUa+eEuM fig8 ------- COMMENT: c1083ee2230b687c 2 itBl/ZwCPKl4Z6MS+EKDWDUdriE (Figure 1A) In normally growing cells, Swi5-EGFP localized to the nucleus and exhibited diffuse nuclear staining with a few distinct foci ------- COMMENT: c1083ee2230b687c 3 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: c1083ee2230b687c 5 I5GfafQ17aFy/UqNB5tYUZLIqhY On the other hand, the nuclei of sfr1D cells contained Swi5-EGFP foci, ------- COMMENT: c1083ee2230b687c 7 6j7XRpAtL+mSw+on9O1RNuQwFx8 (Figure 1B) The absence of Swi2 or Sfr1 did not affect the cellular expression level of the Swi5-EGFP protein . ------- COMMENT: c1083ee2230b687c 8 6j7XRpAtL+mSw+on9O1RNuQwFx8 (Figure 1B) The absence of Swi2 or Sfr1 did not affect the cellular expression level of the Swi5-EGFP protein . ------- COMMENT: c131e5fc09dccabc 58 8cBKe2DefxLfaIG9dbSALYYFy1E (comment: assayed Cdc20 recruitment) ------- COMMENT: c131e5fc09dccabc 59 8cBKe2DefxLfaIG9dbSALYYFy1E (comment: assayed Cdc20 recruitment) ------- COMMENT: c131e5fc09dccabc 60 8cBKe2DefxLfaIG9dbSALYYFy1E (comment: assayed Cdc20 recruitment) ------- COMMENT: c131e5fc09dccabc 61 8cBKe2DefxLfaIG9dbSALYYFy1E (comment: assayed Cdc20 recruitment) ------- COMMENT: c166fc9a9dd60e8d 1 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: c166fc9a9dd60e8d 2 Ci+bKhUitsutwHeHgf5TiETS96Y Fig1 ------- COMMENT: c166fc9a9dd60e8d 3 Ci+bKhUitsutwHeHgf5TiETS96Y Fig1 ------- COMMENT: c166fc9a9dd60e8d 4 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: c166fc9a9dd60e8d 5 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: c166fc9a9dd60e8d 6 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: c166fc9a9dd60e8d 7 Ci+bKhUitsutwHeHgf5TiETS96Y Fig1 ------- COMMENT: c166fc9a9dd60e8d 8 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: c166fc9a9dd60e8d 9 4cbqhematx1kQz0J39lYeJEIW88 figure S1B ------- COMMENT: c166fc9a9dd60e8d 10 MuABLv2UNPDjnQ77YOVkssbT8G8 figure S1C ------- COMMENT: c166fc9a9dd60e8d 11 o6+WxsZu1ASclTZG4sSa4MxW6Fc Figures2A and 2B ------- COMMENT: c166fc9a9dd60e8d 12 o6+WxsZu1ASclTZG4sSa4MxW6Fc Figures2A and 2B ------- COMMENT: c166fc9a9dd60e8d 13 o6+WxsZu1ASclTZG4sSa4MxW6Fc Figures2A and 2B ------- COMMENT: c166fc9a9dd60e8d 14 o6+WxsZu1ASclTZG4sSa4MxW6Fc Figures2A and 2B ------- COMMENT: c166fc9a9dd60e8d 15 o6+WxsZu1ASclTZG4sSa4MxW6Fc Figures2A and 2B ------- COMMENT: c166fc9a9dd60e8d 16 o6+WxsZu1ASclTZG4sSa4MxW6Fc Figures2A and 2B ------- COMMENT: c166fc9a9dd60e8d 17 uM6l6kGhw/GNiQwZ2bfCToLLvP0 Figures2A and 2B (comment: cortical/tubular) ------- COMMENT: c166fc9a9dd60e8d 18 uM6l6kGhw/GNiQwZ2bfCToLLvP0 Figures2A and 2B (comment: cortical/tubular) ------- COMMENT: c166fc9a9dd60e8d 19 7D4MheRSe0YOGJnHZZYFsJDmxJQ Fig 2D (comment: tubular/cortical) ------- COMMENT: c166fc9a9dd60e8d 20 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: c166fc9a9dd60e8d 21 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: c166fc9a9dd60e8d 22 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: c166fc9a9dd60e8d 23 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: c166fc9a9dd60e8d 24 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: c166fc9a9dd60e8d 25 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: c166fc9a9dd60e8d 26 9AjaGj10lNlEzmJlDnZb/+eXveA (Figure S3A) ------- COMMENT: c166fc9a9dd60e8d 27 OjgS7sKLBynHtjUUToGz9pxgF6U Fig3C (comment: protein distributed in cortex) ------- COMMENT: c166fc9a9dd60e8d 28 CWubwhZVInJYb6e7JYWPSzyNr4c Fig3C ------- COMMENT: c166fc9a9dd60e8d 29 CWubwhZVInJYb6e7JYWPSzyNr4c Fig3C ------- COMMENT: c166fc9a9dd60e8d 30 8Bfe9Cak+Lc8NE4KYnce9AOU+B8 Fig 4E ------- COMMENT: c166fc9a9dd60e8d 36 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: c1c81dc437d962d1 2 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: c1c81dc437d962d1 3 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: c1c81dc437d962d1 4 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: c1c81dc437d962d1 5 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: c1c81dc437d962d1 6 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: c1c81dc437d962d1 7 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: c1c81dc437d962d1 8 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: c1c81dc437d962d1 9 O667UCx0ASHujTPeDrrLDdY4G4w Fig. 1H ------- COMMENT: c1c81dc437d962d1 10 O667UCx0ASHujTPeDrrLDdY4G4w Fig. 1H ------- COMMENT: c1c81dc437d962d1 11 O667UCx0ASHujTPeDrrLDdY4G4w Fig. 1H ------- COMMENT: c1c81dc437d962d1 12 MpML2E+8wq5nwC1YfM2LDELzzPU Fig. 2E ------- COMMENT: c1c81dc437d962d1 13 LX30Pzyp0dXIvIlY0jjx8zyNWPA Fig. 2F ------- COMMENT: c1c81dc437d962d1 14 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: c1c81dc437d962d1 15 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: c1c81dc437d962d1 16 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: c1c81dc437d962d1 22 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: c1c81dc437d962d1 23 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: c1c81dc437d962d1 24 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: c1c81dc437d962d1 25 vkT8uMpiBxgGlmYXEA8V3+LXigM Fig. 4E ------- COMMENT: c1c81dc437d962d1 26 vkT8uMpiBxgGlmYXEA8V3+LXigM Fig. 4E ------- COMMENT: c1c81dc437d962d1 27 dJ55jf9F7w1w+3+W5RpemgHi8WI Fig. 5B and C ------- COMMENT: c1c81dc437d962d1 28 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: c1c81dc437d962d1 29 x66kkEOdt343yrWVNX9DiNxJ5eM Phenotype of rad25 deletion amplified by rex1BD deletion at otr3R10 (Fig. 5B) ------- COMMENT: c1c81dc437d962d1 30 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: c1c81dc437d962d1 31 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: c1c81dc437d962d1 33 6kveAjVr0KMJmKAjY0FD22E3Lek Our genetic assays revealed that Rex1BD acts in an RNAi- independent manner. ------- COMMENT: c1c81dc437d962d1 34 HQ2U30RA2GXkjhMJDoUG6kKlGCQ We observed that overexpressed Rex1BD-GFP was enriched within the nucleus, consistent with its role as a heterochromatin factor (SI Appendix, Fig. S3). ------- COMMENT: c1c81dc437d962d1 35 rJqwfkcBWoRyI092mTALXbe9lts We found that the rex1BDΔ cells were also sensitive to MMS, but relatively mild compared with apn2Δ (SI Appendix, Fig. S4). ------- COMMENT: c1c81dc437d962d1 37 O667UCx0ASHujTPeDrrLDdY4G4w Fig. 1H ------- COMMENT: c1c81dc437d962d1 38 Om8sraI/BXM1FjiF+1VAp1kjUts Fig. 5D ------- COMMENT: c1d8ed0226576290 2 w8joKguSnTe12Jfh5pJWkCs6Y1c (comment: glucosylceramide, galactosylceramide) ------- COMMENT: c1d8ed0226576290 3 ZdAyvbamrQMC3AV6vOcC0KCswRE (comment: CHECK glycosphingolipid transport) ------- COMMENT: c1d9cf8c5062801d 78 SFqRetGMdcDTz3I75ciqs7fdp+I (comment: allele tyep "unknown" because neither nt nor aa position 324 is A) ------- COMMENT: c22f209fe76ffcd8 3 u0xUM2zA3oAbz230gbWjKCF+JFs (comment: both SPBs in early mitosis) ------- COMMENT: c22f209fe76ffcd8 23 OwHEG8aXvEYv6Aczre78lUA/hug Pmo25 formed a complex with Nak1 and was required for both the localization and kinase activity of Nak1. ------- COMMENT: c22f209fe76ffcd8 24 KTJuDP8tKhn1gJutcJ9rY8xUX9o In sharp contrast, on a cdc7-24 or sid1-239 mutant background, no Pmo25 was detected at the mitotic SPB(s) (Figure 8B and C). ------- COMMENT: c22f209fe76ffcd8 25 KTJuDP8tKhn1gJutcJ9rY8xUX9o In sharp contrast, on a cdc7-24 or sid1-239 mutant background, no Pmo25 was detected at the mitotic SPB(s) (Figure 8B and C). ------- COMMENT: c2300e5c031f17ac 35 P9STcvYnCHRqjnvMi6V4BvSxIRM The localization of Poz1, Tpz1 and Rap1 proteins are assayed. ------- COMMENT: c2300e5c031f17ac 44 P9STcvYnCHRqjnvMi6V4BvSxIRM The localization of Poz1, Tpz1 and Rap1 proteins are assayed. ------- COMMENT: c23b5043b024b0a5 1 Wext/+AilK0FFU0g2H8XCmy6AKA (comment: CHECK phosphorylated by cdc2 phosphorylated in G2 phase inhibits nonhomologous end joining S192) ------- COMMENT: c23b5043b024b0a5 2 AmJ8nP1qm16GKpY4yq03TajdsTs (comment: CHECK phosphorylated by cdc2 phosphorylated in G2 phase inhibits nonhomologous end joining T180) ------- COMMENT: c23b5043b024b0a5 3 2qkJRnEqrNCUwCmTy7VKuGlOgTs (comment: CHECK increased end-joining activity in vegetative cells) ------- COMMENT: c23b5043b024b0a5 6 icHx4+4/VLwL3bsNSXVK1d1+wzI (comment: leu1) ------- COMMENT: c23b5043b024b0a5 27 wbVxFo4Dch2R3dkker1R+ML9cuk (comment: This looks like direct regulation because it phosphorylates xlf1) ------- COMMENT: c289620cc356e427 1 4ZYhn8FNH8KfXwbxTZvDuPf7dfE The 􏰂RING, C197A, C199A, and C197A/C199A mutants were mildly temperature sensi- tive but grew normally at 25°C (Figure 2A and data not shown) ------- COMMENT: c289620cc356e427 2 4ZYhn8FNH8KfXwbxTZvDuPf7dfE The 􏰂RING, C197A, C199A, and C197A/C199A mutants were mildly temperature sensi- tive but grew normally at 25°C (Figure 2A and data not shown) ------- COMMENT: c289620cc356e427 3 4ZYhn8FNH8KfXwbxTZvDuPf7dfE The 􏰂RING, C197A, C199A, and C197A/C199A mutants were mildly temperature sensi- tive but grew normally at 25°C (Figure 2A and data not shown) ------- COMMENT: c289620cc356e427 4 4ZYhn8FNH8KfXwbxTZvDuPf7dfE The 􏰂RING, C197A, C199A, and C197A/C199A mutants were mildly temperature sensi- tive but grew normally at 25°C (Figure 2A and data not shown) ------- COMMENT: c289620cc356e427 5 4ZYhn8FNH8KfXwbxTZvDuPf7dfE The 􏰂RING, C197A, C199A, and C197A/C199A mutants were mildly temperature sensi- tive but grew normally at 25°C (Figure 2A and data not shown) ------- COMMENT: c289620cc356e427 6 4ZYhn8FNH8KfXwbxTZvDuPf7dfE The 􏰂RING, C197A, C199A, and C197A/C199A mutants were mildly temperature sensi- tive but grew normally at 25°C (Figure 2A and data not shown) ------- COMMENT: c289620cc356e427 7 taFtyR5SfFg5RRU3UN7yVk/NtR0 The C219A mutant was not temperature sensitive (Figure 2A). ------- COMMENT: c289620cc356e427 16 MSVKX0U9X+OmRlhttoTLZpGWwT8 On the basis of these data, we propose that the Nse1 NH-RING contributes to the DNA repair functions of the Smc5-Smc6 holocomplex. ------- COMMENT: c289620cc356e427 18 4pGq7ctSMHeDwPch8tRDjgEK0s0 (comment: nulcleolus inheritance) ------- COMMENT: c289620cc356e427 19 UNMegvNeOiFb81iZMwN6mfvAycE (comment: CHECK synthetic sick when combined with a deletion of the Holliday junction endonuclease Mus81) (Figure 2, C and D). ------- COMMENT: c289620cc356e427 20 UNMegvNeOiFb81iZMwN6mfvAycE (comment: CHECK synthetic sick when combined with a deletion of the Holliday junction endonuclease Mus81) (Figure 2, C and D). ------- COMMENT: c289620cc356e427 24 4vaO3cfjhRKzSsVX70dzvhxeFEs The Nse1-Nse3 interaction is not perturbed by dele- tion of Nse1 NH-RING as tested by yeast two-hybrid assay, which is consistent with a previous report (Figure 5B and Sergeant et al., 2005). ------- COMMENT: c29c5bd54094a6f5 3 89Gp5tOr2E0HW7YUiz+Nkz4HR/I 4′,6-Diamidino-2-phenylindole (DAPI) staining of cultures grown at 25°C showed that ∼10% of nse1-15 cells display chromosome segregation defects, which rises to ∼40% of cells after two cell cycles at 36°C (Figure 1C ------- COMMENT: c29c5bd54094a6f5 4 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: c29c5bd54094a6f5 5 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: c29c5bd54094a6f5 6 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: c29c5bd54094a6f5 7 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: c29c5bd54094a6f5 8 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: c29c5bd54094a6f5 9 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: c29c5bd54094a6f5 10 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: c29c5bd54094a6f5 11 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: c29c5bd54094a6f5 12 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: c29c5bd54094a6f5 13 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: c29c5bd54094a6f5 14 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: c29c5bd54094a6f5 15 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: c29c5bd54094a6f5 16 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: c29c5bd54094a6f5 17 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: c29c5bd54094a6f5 18 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: c29c5bd54094a6f5 19 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: c29c5bd54094a6f5 20 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: c29c5bd54094a6f5 21 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: c29c5bd54094a6f5 22 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: c29c5bd54094a6f5 23 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: c29c5bd54094a6f5 24 hXCzSAh5nGw+ltXuSW+wwVG5aG4 We found that nse1-C216S suppressed the MMS, HU, and UV sen- sitivity of both smc6-74 and smc6-X, almost back to wild-type levels (Figure 2, C and D). ------- COMMENT: c29c5bd54094a6f5 25 hXCzSAh5nGw+ltXuSW+wwVG5aG4 We found that nse1-C216S suppressed the MMS, HU, and UV sen- sitivity of both smc6-74 and smc6-X, almost back to wild-type levels (Figure 2, C and D). ------- COMMENT: c29c5bd54094a6f5 26 hXCzSAh5nGw+ltXuSW+wwVG5aG4 We found that nse1-C216S suppressed the MMS, HU, and UV sen- sitivity of both smc6-74 and smc6-X, almost back to wild-type levels (Figure 2, C and D). ------- COMMENT: c29c5bd54094a6f5 27 hXCzSAh5nGw+ltXuSW+wwVG5aG4 We found that nse1-C216S suppressed the MMS, HU, and UV sen- sitivity of both smc6-74 and smc6-X, almost back to wild-type levels (Figure 2, C and D). ------- COMMENT: c29c5bd54094a6f5 28 hXCzSAh5nGw+ltXuSW+wwVG5aG4 We found that nse1-C216S suppressed the MMS, HU, and UV sen- sitivity of both smc6-74 and smc6-X, almost back to wild-type levels (Figure 2, C and D). ------- COMMENT: c29c5bd54094a6f5 29 hXCzSAh5nGw+ltXuSW+wwVG5aG4 We found that nse1-C216S suppressed the MMS, HU, and UV sen- sitivity of both smc6-74 and smc6-X, almost back to wild-type levels (Figure 2, C and D). ------- COMMENT: c29c5bd54094a6f5 30 hXCzSAh5nGw+ltXuSW+wwVG5aG4 We found that nse1-C216S suppressed the MMS, HU, and UV sen- sitivity of both smc6-74 and smc6-X, almost back to wild-type levels (Figure 2, C and D). ------- COMMENT: c29c5bd54094a6f5 31 hXCzSAh5nGw+ltXuSW+wwVG5aG4 We found that nse1-C216S suppressed the MMS, HU, and UV sen- sitivity of both smc6-74 and smc6-X, almost back to wild-type levels (Figure 2, C and D). ------- COMMENT: c29c5bd54094a6f5 32 tX6mmFSczHMk23NUqZop4cWQVTc Remarkably, nse1-C216S sup- pressed the synthetic lethality of brc1Δ smc6-74 cells but did not suppress the sen- sitivity of brc1Δ smc6-74 cells to MMS and UV irradiation. Moreover, nse1-C216S also suppressed the MMS sensitivity of brc1Δ cells (Figure 4) ------- COMMENT: c29c5bd54094a6f5 33 tX6mmFSczHMk23NUqZop4cWQVTc Remarkably, nse1-C216S sup- pressed the synthetic lethality of brc1Δ smc6-74 cells but did not suppress the sen- sitivity of brc1Δ smc6-74 cells to MMS and UV irradiation. Moreover, nse1-C216S also suppressed the MMS sensitivity of brc1Δ cells (Figure 4) ------- COMMENT: c29c5bd54094a6f5 34 3LsoUvkNy06PoqXLtbydF2ToDoU Remarkably, rhp57Δ nse1-C216S double mutants were significantly more sensitive to MMS and HU than the rhp57Δ parent. Furthermore, rhp57Δ smc6-74 nse1-C216S triple mutants were more sensitive than smc6-74 rhp57Δ cells (Figure 5, A and B). ------- COMMENT: c29c5bd54094a6f5 35 3LsoUvkNy06PoqXLtbydF2ToDoU Remarkably, rhp57Δ nse1-C216S double mutants were significantly more sensitive to MMS and HU than the rhp57Δ parent. Furthermore, rhp57Δ smc6-74 nse1-C216S triple mutants were more sensitive than smc6-74 rhp57Δ cells (Figure 5, A and B). ------- COMMENT: c29c5bd54094a6f5 36 3LsoUvkNy06PoqXLtbydF2ToDoU Remarkably, rhp57Δ nse1-C216S double mutants were significantly more sensitive to MMS and HU than the rhp57Δ parent. Furthermore, rhp57Δ smc6-74 nse1-C216S triple mutants were more sensitive than smc6-74 rhp57Δ cells (Figure 5, A and B). ------- COMMENT: c29d60efe6a85457 4 aSH1UoHehaOSoogIzECd67T4pn4 figure 1 ------- COMMENT: c29d60efe6a85457 5 aSH1UoHehaOSoogIzECd67T4pn4 figure 1 ------- COMMENT: c29d60efe6a85457 6 2kWrYgCff0AdqCoEB51+XPZcl0Q Supplemental Table S1; Supplemental Fig. S3 ------- COMMENT: c29d60efe6a85457 7 2kWrYgCff0AdqCoEB51+XPZcl0Q Supplemental Table S1; Supplemental Fig. S3 ------- COMMENT: c29d60efe6a85457 8 2kWrYgCff0AdqCoEB51+XPZcl0Q Supplemental Table S1; Supplemental Fig. S3 ------- COMMENT: c29d60efe6a85457 9 2kWrYgCff0AdqCoEB51+XPZcl0Q Supplemental Table S1; Supplemental Fig. S3 ------- COMMENT: c29d60efe6a85457 10 2kWrYgCff0AdqCoEB51+XPZcl0Q Supplemental Table S1; Supplemental Fig. S3 ------- COMMENT: c29d60efe6a85457 11 AVBEgV2yJW+UH4NSlcUVH01lTtA (Supplemental Table S1). ------- COMMENT: c29d60efe6a85457 12 AVBEgV2yJW+UH4NSlcUVH01lTtA (Supplemental Table S1). ------- COMMENT: c29d60efe6a85457 14 AVBEgV2yJW+UH4NSlcUVH01lTtA (Supplemental Table S1). ------- COMMENT: c29d60efe6a85457 15 AVBEgV2yJW+UH4NSlcUVH01lTtA (Supplemental Table S1). ------- COMMENT: c29d60efe6a85457 16 AVBEgV2yJW+UH4NSlcUVH01lTtA (Supplemental Table S1). ------- COMMENT: c29d60efe6a85457 17 AVBEgV2yJW+UH4NSlcUVH01lTtA (Supplemental Table S1). ------- COMMENT: c29d60efe6a85457 18 AVBEgV2yJW+UH4NSlcUVH01lTtA (Supplemental Table S1). ------- COMMENT: c29d60efe6a85457 20 H0MnaHI1FiMjP1CYNeqjyYIN72Q figure 5A ------- COMMENT: c29d60efe6a85457 21 H0MnaHI1FiMjP1CYNeqjyYIN72Q figure 5A ------- COMMENT: c29d60efe6a85457 22 H0MnaHI1FiMjP1CYNeqjyYIN72Q figure 5A ------- COMMENT: c29d60efe6a85457 23 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: c29d60efe6a85457 24 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: c29d60efe6a85457 25 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: c29f51c4cc86096d 19 a49Yn1fpcSYHDQmTCr6McJg9Ev8 (comment: punctate; shorter duration of heat exposure (up to ~45 min)) ------- COMMENT: c29f51c4cc86096d 37 gZvKJtr3W0lvV2hkYPoXoK5NjsU (comment: 36 degrees (not brief heat shock)) ------- COMMENT: c29f51c4cc86096d 72 YbZ/m4TxEMAQDS8ve9us/VHJUnc Global gene expression profile (RNAseq) of deletion similar to that of heat-stressed wild type. ------- COMMENT: c29f51c4cc86096d 74 YbZ/m4TxEMAQDS8ve9us/VHJUnc Global gene expression profile (RNAseq) of deletion similar to that of heat-stressed wild type. ------- COMMENT: c29f51c4cc86096d 75 mdyyAoF429gC/ETa9uJrs4F0V4Y (comment: CONDITION prolonged heat exposure (more than ~45 min)) ------- COMMENT: c29f51c4cc86096d 76 mdyyAoF429gC/ETa9uJrs4F0V4Y (comment: CONDITION prolonged heat exposure (more than ~45 min)) ------- COMMENT: c29f51c4cc86096d 77 mdyyAoF429gC/ETa9uJrs4F0V4Y (comment: CONDITION prolonged heat exposure (more than ~45 min)) ------- COMMENT: c29f51c4cc86096d 78 mdyyAoF429gC/ETa9uJrs4F0V4Y (comment: CONDITION prolonged heat exposure (more than ~45 min)) ------- COMMENT: c29f51c4cc86096d 79 mdyyAoF429gC/ETa9uJrs4F0V4Y (comment: CONDITION prolonged heat exposure (more than ~45 min)) ------- COMMENT: c29f51c4cc86096d 80 mdyyAoF429gC/ETa9uJrs4F0V4Y (comment: CONDITION prolonged heat exposure (more than ~45 min)) ------- COMMENT: c29f51c4cc86096d 81 Avn0x+4ypVOcm8bW5Z+hfBgCy8k (comment: CONDITION shorter duration of heat exposure (up to ~45 min)) ------- COMMENT: c29f51c4cc86096d 82 Avn0x+4ypVOcm8bW5Z+hfBgCy8k (comment: CONDITION shorter duration of heat exposure (up to ~45 min)) ------- COMMENT: c29f51c4cc86096d 84 Avn0x+4ypVOcm8bW5Z+hfBgCy8k (comment: CONDITION shorter duration of heat exposure (up to ~45 min)) ------- COMMENT: c29f51c4cc86096d 95 eiKQtabM16wRLbGopOX4bUQMogE "Both Gef1-3YFP and Scd1-GFP exhibited bipolar localization in majority of late wild type cells" ------- COMMENT: c29f51c4cc86096d 96 eiKQtabM16wRLbGopOX4bUQMogE "Both Gef1-3YFP and Scd1-GFP exhibited bipolar localization in majority of late wild type cells" ------- COMMENT: c2a157dbb0ccbaa8 4 alUoAexMbZqxpsYT87pu3557a60 (comment: ocalization independent of actin cytoskeleton (assayed using latrunculin A) and microtubule cytoskeleton (assayed using carbendazim)) ------- COMMENT: c2a157dbb0ccbaa8 5 alUoAexMbZqxpsYT87pu3557a60 (comment: ocalization independent of actin cytoskeleton (assayed using latrunculin A) and microtubule cytoskeleton (assayed using carbendazim)) ------- COMMENT: c2a8d0f860ace791 2 jQJP4uTaWoKW/emFzOCsPw3Ej7o (comment: not sure if this is quite right) ------- COMMENT: c2a8d0f860ace791 5 +UCr9i3DdZ6DOElnMpOvU+p3GC8 (comment: VW: added exists_during..) ------- COMMENT: c2a8d0f860ace791 6 a19tI1BlbH8BEtJY40Vl1q1lzcM Figure S1G ------- COMMENT: c2a8d0f860ace791 7 /ra7Dgo9Xvh+XUyfGWLaJvu6YCc Figure S1F ------- COMMENT: c2a8d0f860ace791 8 CWDNOTCg8XZXu3wN+tnGCm7OgGg Figure 1B, Figure S1F ------- COMMENT: c2a8d0f860ace791 9 CWDNOTCg8XZXu3wN+tnGCm7OgGg Figure 1B, Figure S1F ------- COMMENT: c2a8d0f860ace791 11 ElCEw4aIE3PdJGE72icI5g5k5gY Figures 2B and 2C ------- COMMENT: c2a8d0f860ace791 13 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: c2a8d0f860ace791 20 eNIIbCtFGqx6v/ARyjobdSYZ9NE Figure 7C) ------- COMMENT: c2a8d0f860ace791 21 RyWCtZjhsbVv7agleVIo/30Xk08 Fig- ure 7D ------- COMMENT: c2a8d0f860ace791 23 x7s04GdE/CAYKhDMjVI2pjppDKc Figure 1A. Figures S1C–S1E ------- COMMENT: c2a8d0f860ace791 24 Boxd8w6nNLAaaFaCg+g7K4omVJs Figures S1A and S1B ------- COMMENT: c2a8d0f860ace791 26 PF+C3OzDZ55NyrW3vj8lePI0K3M Figures S2A and S2B ------- COMMENT: c2a8d0f860ace791 27 amkIPLDQVnr4netHXDBnEknA370 Figures 1F and S2B ------- COMMENT: c2a8d0f860ace791 28 Mc1Dl/8ePjG+W3BQIevC1vaEvqw Figure 2D ------- COMMENT: c2a8d0f860ace791 29 cYkrsU/4y6MhVbVmPef6qIMhVpg Figures 2D and S3B ------- COMMENT: c2a8d0f860ace791 31 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: c2a8d0f860ace791 39 b+00gXuAJVmG6MoHxSub6s9WxVw Figure 4C ------- COMMENT: c2a8d0f860ace791 40 /lDA8q2quyiq/hY1oP4bUs4SfBs Figure S5B) ------- COMMENT: c2a8d0f860ace791 41 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: c2a8d0f860ace791 43 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: c2a8d0f860ace791 44 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: c2a8d0f860ace791 45 crdk8+5Su6ZjOoDfvYBv9bmPLs0 Figure 5C ------- COMMENT: c2a8d0f860ace791 46 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: c2a8d0f860ace791 47 crdk8+5Su6ZjOoDfvYBv9bmPLs0 Figure 5C ------- COMMENT: c2a8d0f860ace791 48 crdk8+5Su6ZjOoDfvYBv9bmPLs0 Figure 5C ------- COMMENT: c2a8d0f860ace791 50 lewWnS5goUq8jT/WZM96TE1+g7Q Figure 7B ------- COMMENT: c2a8d0f860ace791 51 RtBvVrITD8NDjgq945mAizXFajU Fig. 7e ------- COMMENT: c2a8d0f860ace791 52 ypCyrLQ4g1YSxpZXtumJe3ZV4Sk Fig. 7F ------- COMMENT: c2aaa605ef48b5f8 35 M+bk2tEwG1ALNdF/1ReSMFAiO0I (comment: CHECK un- ubiquitinated) ------- COMMENT: c2cd1d8a4d29ba93 25 RqGcZ6u3PScRW1nQ7yFCQZnQogM (comment: Cup1-GFP immunofluorescence) ------- COMMENT: c2d743509dce790c 13 n3RqKXoZ8ODwmkiFTWfqQ6Rhq6g (comment: vw: delayed) ------- COMMENT: c2d743509dce790c 34 2PhNEXDi5y5XAD9bKhX/9MRUK80 (comment: vw: DNA checkpoint dept) ------- COMMENT: c2d743509dce790c 45 15SE8+2IQYgIiJRUMvJmezRfijI (comment: vw: I added this as an inference, because the checkpoint is never satisfied) ------- COMMENT: c2d743509dce790c 46 15SE8+2IQYgIiJRUMvJmezRfijI (comment: vw: I added this as an inference, because the checkpoint is never satisfied) ------- COMMENT: c33d429f18cfda43 1 oyELVTb2UF7y9n7On94jcJdTnrY (comment: CHECK ark1-TS) ------- COMMENT: c33d429f18cfda43 2 Am0kjmUysRAWL/4jN3S5VzotyGw Figure 1a ------- COMMENT: c33d429f18cfda43 3 E3HXkGKmAvMiN8QFdhDGwf50Fnc figure 1b ------- COMMENT: c33d429f18cfda43 4 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: c33d429f18cfda43 5 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: c33d429f18cfda43 6 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: c33d429f18cfda43 7 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: c33d429f18cfda43 9 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: c33d429f18cfda43 10 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: c33d429f18cfda43 11 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: c33d429f18cfda43 12 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: c33d429f18cfda43 13 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: c33d429f18cfda43 14 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: c396e950a0cdf1ad 7 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: c396e950a0cdf1ad 9 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: c396e950a0cdf1ad 11 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: c396e950a0cdf1ad 12 GckyyX1S7lB71dx2la5yFUbJ99o Fig 1C (comment: vw data not shown, but assume are elongated) ------- COMMENT: c396e950a0cdf1ad 13 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: c396e950a0cdf1ad 14 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: c396e950a0cdf1ad 18 m2urrNB5df2Dj8iUz+pAN9xHVhA Figure 2B lower right panel. rad3 is not present in the chromatin fraction in the absence of cdc18 ------- COMMENT: c396e950a0cdf1ad 19 SS5ncxdUOLu9S/54tXt4GPYzX/o Figure 2B upper right panel. In the cytosol rad3 is present in absence of cdc18 ------- COMMENT: c396e950a0cdf1ad 20 cuxfpnXzaFlQ81SejObQs5qZRcw Figure 2C lower panel. cds1 is no longer phosphorylated because rad3 is absent in absence of cdc18 ------- COMMENT: c396e950a0cdf1ad 21 bkFO6nWykRbxSMxtQa0QE4m/4t0 Figure 2D top of lower panel. ------- COMMENT: c396e950a0cdf1ad 23 mqwo/7kAJX1CYdFWNwKrVEsmLJI Fig3 top 2 two panels (ve jacky syuggested Presence of stalled replication forks after DNA checkpoint inactivation, i just used normal initiation Figure 3. Replication Structures Are Not Lost When Cdc18 Is Depleted) ------- COMMENT: c396e950a0cdf1ad 24 +t2qCCGlK6xrXs1Rvm4vTpyZddI Fig 4A ------- COMMENT: c396e950a0cdf1ad 25 +t2qCCGlK6xrXs1Rvm4vTpyZddI Fig 4A ------- COMMENT: c396e950a0cdf1ad 28 RixlvlgTmMBOZ93/xdU305iyOZI Fig 4A ------- COMMENT: c396e950a0cdf1ad 29 RixlvlgTmMBOZ93/xdU305iyOZI Fig 4A ------- COMMENT: c396e950a0cdf1ad 31 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: c396e950a0cdf1ad 32 XyysdOWjUS8RfK7gvtte7sm9nZY Fig 4D In the absence of rad26, cdc18 is unable to stabilise rad3 on chromatin ------- COMMENT: c396e950a0cdf1ad 33 zwFpxxPzKyKBjldRctmg+nW06Ss Fig5A cdc18 disappears at the end of S-phase in cig2+ strain and accumulates in the absence of cig2 ------- COMMENT: c396e950a0cdf1ad 34 UGKLbMSoFrW9s5nNAMBghTw/TiA Fig 5B ------- COMMENT: c396e950a0cdf1ad 35 UGKLbMSoFrW9s5nNAMBghTw/TiA Fig 5B ------- COMMENT: c396e950a0cdf1ad 36 GKaoaDNvmiscHEUM+1GYrgxCfJ4 Fig 6B ------- COMMENT: c396e950a0cdf1ad 37 t06dwlfQjN9Tx1OFuUhULmgZu5c Fig 6B ------- COMMENT: c396e950a0cdf1ad 38 t06dwlfQjN9Tx1OFuUhULmgZu5c Fig 6B ------- COMMENT: c396e950a0cdf1ad 42 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: c396e950a0cdf1ad 43 mII7sUI1uVaA4Lid7ncQvuLk/Gk Fig. 2A; (comment: CHECK during mitotic DNA replication checkpoint) ------- COMMENT: c396e950a0cdf1ad 44 ernVuLQ3ugzbI6GXr4Wb6RsgMe4 Fig. 2A ------- COMMENT: c396e950a0cdf1ad 45 DHvP50k7vqSCqJKNkYTUl5FHISI The same experiment was repeated, but HU was added at the time of release, allowing cells to progress through mitosis and stall after initiation of DNA replication. In that case, Cdc2 tyrosine 15 phosphorylation reappeared after 100 min and increased further with time. Cig2 remained present at a high level, indicating that the Cdc2-Cig2 com- plex was inhibited (Figure 5B) ------- COMMENT: c3b8098ab8181f07 1 joUktSPUGXl1ezDJB/dL25jTXFA figure1 ------- COMMENT: c3b8098ab8181f07 5 joUktSPUGXl1ezDJB/dL25jTXFA figure1 ------- COMMENT: c3b8098ab8181f07 6 joUktSPUGXl1ezDJB/dL25jTXFA figure1 ------- COMMENT: c3b8098ab8181f07 7 joUktSPUGXl1ezDJB/dL25jTXFA figure1 ------- COMMENT: c3b8098ab8181f07 8 jTI9ygFTx+A/g+CTEJ4UVC2E9YU git2􏰇 strains themselves were severely defective for growth on YEA medium containing 1 M KCl (Fig. 1). ------- COMMENT: c3b8098ab8181f07 9 NmZvQXDa97u1Wfg7SzNorGliTv4 unlike JSP12 (git2􏰇 spc1-12) and JSP29 (git2􏰇 wis1-29), cells that elongate and die, cells of the parental strain FWP190 (git2􏰇) appear to simply arrest growth (Fig. 2) ------- COMMENT: c3b8098ab8181f07 10 BcMypYvwaLSjaZS/r7aYmOXmwYw figure2 ------- COMMENT: c3b8098ab8181f07 11 BcMypYvwaLSjaZS/r7aYmOXmwYw figure2 ------- COMMENT: c3b8098ab8181f07 12 BcMypYvwaLSjaZS/r7aYmOXmwYw figure2 ------- COMMENT: c3b8098ab8181f07 13 b0MV5PX/Hy2thGXOLwMfA9/sG8g he FWP190 (git2􏰇) cells can grow in the pres- ence of 2 M sorbitol, which is lethal to JSP12 (git2􏰇 spc1-12) and JSP29 (git2􏰇 wis1-29) cells. Therefore, the loss of adenyl- ate cyclase appears to confer a salt-sensitive, but not an os- motically sensitive, growth defect. ------- COMMENT: c3b8098ab8181f07 14 b0MV5PX/Hy2thGXOLwMfA9/sG8g he FWP190 (git2􏰇) cells can grow in the pres- ence of 2 M sorbitol, which is lethal to JSP12 (git2􏰇 spc1-12) and JSP29 (git2􏰇 wis1-29) cells. Therefore, the loss of adenyl- ate cyclase appears to confer a salt-sensitive, but not an os- motically sensitive, growth defect. ------- COMMENT: c3b8098ab8181f07 15 b0MV5PX/Hy2thGXOLwMfA9/sG8g he FWP190 (git2􏰇) cells can grow in the pres- ence of 2 M sorbitol, which is lethal to JSP12 (git2􏰇 spc1-12) and JSP29 (git2􏰇 wis1-29) cells. Therefore, the loss of adenyl- ate cyclase appears to confer a salt-sensitive, but not an os- motically sensitive, growth defect. ------- COMMENT: c3b8098ab8181f07 16 b0MV5PX/Hy2thGXOLwMfA9/sG8g he FWP190 (git2􏰇) cells can grow in the pres- ence of 2 M sorbitol, which is lethal to JSP12 (git2􏰇 spc1-12) and JSP29 (git2􏰇 wis1-29) cells. Therefore, the loss of adenyl- ate cyclase appears to confer a salt-sensitive, but not an os- motically sensitive, growth defect. ------- COMMENT: c3b8098ab8181f07 17 j4mUB1OTi6yESvcWT9dkH5/HwX8 For cgs1-1 mu- tant cells, pregrowth on YEA medium results in a nearly 100- fold loss of mating efficiency, as expected. More surprisingly, we found that pregrowth of the cgs1-1 strain on PM medium reduces the mating efficiency defect to only fourfold (Table 2). ------- COMMENT: c3b8098ab8181f07 18 j4mUB1OTi6yESvcWT9dkH5/HwX8 For cgs1-1 mu- tant cells, pregrowth on YEA medium results in a nearly 100- fold loss of mating efficiency, as expected. More surprisingly, we found that pregrowth of the cgs1-1 strain on PM medium reduces the mating efficiency defect to only fourfold (Table 2). ------- COMMENT: c3b8098ab8181f07 19 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: c3b8098ab8181f07 20 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: c3b8098ab8181f07 21 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: c3b8098ab8181f07 22 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: c3b8098ab8181f07 23 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: c3b8098ab8181f07 24 hQqbdkFhynnTdHzII0cazcBO4VQ table 3 ------- COMMENT: c3b8098ab8181f07 25 hQqbdkFhynnTdHzII0cazcBO4VQ table 3 ------- COMMENT: c3b8098ab8181f07 26 JpZxuExtaYMjFoZ2RmYVpe2Xjw0 Fig. 7 (lanes 1 and 2), we observed that both cgs1􏰀 and pka1􏰀 are transcriptionally induced by glucose starvation. ------- COMMENT: c3b8098ab8181f07 27 JpZxuExtaYMjFoZ2RmYVpe2Xjw0 Fig. 7 (lanes 1 and 2), we observed that both cgs1􏰀 and pka1􏰀 are transcriptionally induced by glucose starvation. ------- COMMENT: c3b8098ab8181f07 28 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: c3b8098ab8181f07 29 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: c3b8098ab8181f07 30 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: c3b8098ab8181f07 31 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: c3b81f2e0da5b138 11 bhSWc6l3XUxembpY1KdSchuFnEo Fig. 3f ------- COMMENT: c3b81f2e0da5b138 12 0P98av7voDlSBufur40fEQz6QvY Fig. 3f In marked contrast, subtelomeric tlh transcripts (Figure 3a) accumulated in chp1ΔC strains (Figure 3b,e Supplementary Figure 9). ------- COMMENT: c3b81f2e0da5b138 13 keW063NA53Cu9sORFJ1tFjcbE5g Fig. 3g ------- COMMENT: c3b81f2e0da5b138 14 cJUeeUF/ygQfb7IJvbU/wEbdCLI In marked contrast, subtelomeric tlh transcripts (Figure 3a) accumulated in chp1ΔC strains (Figure 3b,e Supplementary Figure 9). ------- COMMENT: c3b81f2e0da5b138 15 GMN6pDnzS5vp0U6V+RrRNTjZD1c Real time PCR analyses of transcripts derived from the dg or dh outer repeats of the centromere (Figure 3a) demonstrated that whereas cells lacking Chp1 displayed strong accumulation of transcripts, centromeric heterochromatin was unaffected by the loss of the PIN domain in chp1ΔC strains (Figure 3b,c,d Supplementary Figure 9). ------- COMMENT: c3eceb1486a6bc53 1 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: c3eceb1486a6bc53 2 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: c3eceb1486a6bc53 4 LdT0iyjTyv+jx+OPYDmUgE5cT3U We also examined cell viability of stationary phase rgf2D and rgf21 cultures incubated for 4 days at 28°; both strains were found to be .90% viable during this period. ------- COMMENT: c3eceb1486a6bc53 5 BQLepvu2yK6otDlr9++5FGD+9Lw 90% viability ? We also examined cell viability of stationary phase rgf2D and rgf21 cultures incubated for 4 days at 28°; both strains were found to be .90% viable during this period. ------- COMMENT: c3eceb1486a6bc53 7 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: c3eceb1486a6bc53 8 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: c3eceb1486a6bc53 11 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: c3eceb1486a6bc53 12 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: c3eceb1486a6bc53 13 cIb3Za6y+h8N9WcDRGQnYaHA5XQ Figure 2b ------- COMMENT: c3eceb1486a6bc53 14 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: c3eceb1486a6bc53 15 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: c3eceb1486a6bc53 17 S3V/Kie1RjoVZ3JPCVTPm0jH2AE Figure 3 "This result indicates that Rgf2p is in- volved in b-glucan biosynthesis during sporulation." figure4c These results clearly indicate that Rgf2p is involved in the regulation of b(1,3)-glucan biosynthesis. ------- COMMENT: c3eceb1486a6bc53 18 9flObMFM23QotoxR+cvR0pCCf8k Figure 4c (comment: positive ------- COMMENT: c3eceb1486a6bc53 19 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: c3eceb1486a6bc53 20 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: c3eceb1486a6bc53 21 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: c3eceb1486a6bc53 23 kx/ugTe2Efop0Wbiz0U7fBVGTbA the amount of active Rho1p increased considerably in the strain over- expressing Rgf2p as compared with the wild-type strain (Figure 4B ------- COMMENT: c3eceb1486a6bc53 24 9zUuz1qtwc7hrQD85wk3Osqy9V4 Asexpected,over- expression of the rgf2-PTTRD mutant in a pREP3X vector produced viable cells and no multiseptated phenotype was seen, even at very long times of derepresion in the absence of thiamine (Figure 4A). ------- COMMENT: c3eceb1486a6bc53 25 VrqJXPds7UQZABff0k2uzLuwwag cells were larger than wild-type cells and displayed multiple abnormal septa. ------- COMMENT: c3eceb1486a6bc53 26 PfHSLM891Xn495b6MwgiKnb/3iQ GS activity was threefold higher than that observed in the wild-type strain (Figure 4C) ------- COMMENT: c3eceb1486a6bc53 27 PfHSLM891Xn495b6MwgiKnb/3iQ GS activity was threefold higher than that observed in the wild-type strain (Figure 4C) ------- COMMENT: c3eceb1486a6bc53 28 y0CBtTOylIr/GP8pfutls4ZlA1c None of the 11 spores predicted to be rgf1This31 rgf2Tura1 was viable, strongly supporting the idea that simultaneous disruption of rgf11 and rgf21 is lethal. To eliminate the possibility that these mutations might be affecting only sporulation or germination, we also tested for synthetic lethality during vegetative growth ------- COMMENT: c3eceb1486a6bc53 38 eDiYlHR8dmv1ZYPMTFEUaHh8u2M Figure 5B viable and phenotypically in- distinguishable from the ehs2-1 mutant ------- COMMENT: c3eceb1486a6bc53 39 TcgygI/WgUovgCbBCeo123PUVoU Figue 5B As expected for the rgf31 shut-off, the cells died in the presence of thiamine (promoter off). ------- COMMENT: c3eceb1486a6bc53 40 LtYrgRd7rntYDKI8GyCSU3kCoxQ Figue 5B How- ever, their growth was rescued in the presence of sorbitol ------- COMMENT: c3eceb1486a6bc53 41 TcgygI/WgUovgCbBCeo123PUVoU Figue 5B As expected for the rgf31 shut-off, the cells died in the presence of thiamine (promoter off). ------- COMMENT: c3eceb1486a6bc53 42 LtYrgRd7rntYDKI8GyCSU3kCoxQ Figue 5B How- ever, their growth was rescued in the presence of sorbitol ------- COMMENT: c3eceb1486a6bc53 43 WG6QSDfETNnouGEv5jRRF6VkPLU Our results indicate that Rgf1p and Rgf2p share an essential role as Rho1p activators, and they suggest that in the absence of Rgf1p, Rgf2p takes over the essential functions for Rho1p during vegetative growth. ------- COMMENT: c3eceb1486a6bc53 44 7NLLy3v6WhJcChlKv7VJMqQEQbk Figure 1 Rgf2p, a Rho1-GEF Required for Sporulation in S. pombe 1329 6A (top) shows that rgf2 expressed from plasmids, containing the rgf21 genomic promoter (pGR13) or the strongest nmt1 promoter (pGR70), fully rescued the lysis and the Csp hypersensitivity of rgf1D cells in medium containing thiamine. ------- COMMENT: c3eceb1486a6bc53 45 tThhdMz1W9J1jbFnvZ38rze7nck (comment: CHECKB 47% of cells) ------- COMMENT: c3eceb1486a6bc53 46 N36G3S9I1o3O/8n6H2x8hdaNE8E (comment: CHECK 10% of cells) ------- COMMENT: c3eceb1486a6bc53 47 WG6QSDfETNnouGEv5jRRF6VkPLU Our results indicate that Rgf1p and Rgf2p share an essential role as Rho1p activators, and they suggest that in the absence of Rgf1p, Rgf2p takes over the essential functions for Rho1p during vegetative growth. ------- COMMENT: c3f91b7bec8a34a1 32 Mrd9tMFOdMiTlR1mUYkNcHawTiA fig7, (comment: sort of indirect - kinase dead mutant doesn't activate) ------- COMMENT: c3f91b7bec8a34a1 33 fFh7c9kQEloiTVlfghyLG2odtBw (comment: This one comes in "from the side", see Ladds, Bond post 2010 publication summary) ------- COMMENT: c40f69f244d75cea 1 d5zmRf5be9vU1wenojfK6hH/tcs (Fig. 1C and D) ------- COMMENT: c40f69f244d75cea 3 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: c40f69f244d75cea 4 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: c40f69f244d75cea 6 cgUZ9Rgpi1C57s7n5arqT9ZUWoE (Fig. 2B and C) ------- COMMENT: c40f69f244d75cea 7 QoKGwdnKwghslxWxKChYDvJGWbg Dataset S2 ------- COMMENT: c40f69f244d75cea 8 QoKGwdnKwghslxWxKChYDvJGWbg Dataset S2 ------- COMMENT: c40f69f244d75cea 9 HVLL9zNnsbJYh5//n8vC6gJyClM (Fig. S10A) ------- COMMENT: c40f69f244d75cea 11 40bhCqeMD1zeOaeLUSaSnnsIMJw (Fig. 3B and C) ------- COMMENT: c40f69f244d75cea 13 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: c40f69f244d75cea 14 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: c40f69f244d75cea 16 GkI93EYSqP6NybAtsJjxO2fy3Ic (Fig. 4D) ------- COMMENT: c40f69f244d75cea 17 GkI93EYSqP6NybAtsJjxO2fy3Ic (Fig. 4D) ------- COMMENT: c40f69f244d75cea 18 GkI93EYSqP6NybAtsJjxO2fy3Ic (Fig. 4D) ------- COMMENT: c40f69f244d75cea 19 VHdSfGbndL4Z/x07bz5TMyOMuP4 (Fig. 4K) ------- COMMENT: c40f69f244d75cea 20 VHdSfGbndL4Z/x07bz5TMyOMuP4 (Fig. 4K) ------- COMMENT: c40f69f244d75cea 21 VHdSfGbndL4Z/x07bz5TMyOMuP4 (Fig. 4K) ------- COMMENT: c40f69f244d75cea 22 VHdSfGbndL4Z/x07bz5TMyOMuP4 (Fig. 4K) ------- COMMENT: c40f69f244d75cea 23 VHdSfGbndL4Z/x07bz5TMyOMuP4 (Fig. 4K) ------- COMMENT: c40f69f244d75cea 24 VHdSfGbndL4Z/x07bz5TMyOMuP4 (Fig. 4K) ------- COMMENT: c40f69f244d75cea 25 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: c40f69f244d75cea 26 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: c40f69f244d75cea 27 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: c40f69f244d75cea 28 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: c40f69f244d75cea 29 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: c40f69f244d75cea 30 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: c40f69f244d75cea 31 XVHahgPXZsFOyduY4BP8CoHD6gY (Fig. 5E and F) ------- COMMENT: c40f69f244d75cea 32 H/QAYjbv2Dq+FwajtClxAiq6YPw These results suggest that the only role of Bdf1 in Tdk1-mediated killing is to bridge an association between Tdk1 and acetylated histones, leading to the attachment of Tdk1 to chromosomes. (Fig. 6) ------- COMMENT: c40f69f244d75cea 33 BgthRc9U4WbpcxmCrg4yU6vEg20 (Fig. S9C) ------- COMMENT: c40f69f244d75cea 34 PGCuy5xsv4Ra4c9Evl81+cPA35M (Fig. S9D) ------- COMMENT: c40f69f244d75cea 35 HVLL9zNnsbJYh5//n8vC6gJyClM (Fig. S10A) ------- COMMENT: c40f69f244d75cea 36 HVLL9zNnsbJYh5//n8vC6gJyClM (Fig. S10A) ------- COMMENT: c40f69f244d75cea 37 baWpAD8e8itJWdr7paA14exMOMI (Fig. S10B) ------- COMMENT: c40f69f244d75cea 38 MfhXrratyk9HEpPvYNKyPqJLEoI (Fig. 4E) ------- COMMENT: c41da3dd62aad5eb 28 +7VhmJbhpMJCCtDK+62qU+Rh8SI (comment: don't know veg or spore) ------- COMMENT: c4735d3b81a5d777 1 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: c4735d3b81a5d777 4 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: c480fb02b530ca54 1 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: c480fb02b530ca54 2 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: c480fb02b530ca54 3 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: c480fb02b530ca54 4 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: c480fb02b530ca54 5 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: c480fb02b530ca54 6 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: c480fb02b530ca54 7 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: c480fb02b530ca54 9 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: c480fb02b530ca54 10 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: c480fb02b530ca54 11 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: c480fb02b530ca54 12 5dFAlNZxTfCxQYgmImi80i42CcU Table 1 (comment: CHECK wide phenotype suppressed by sorbitol) ------- COMMENT: c480fb02b530ca54 13 wjZExu1SPIyYHykVrnlpGn8ddvA Table 1 (comment: CHECK not suppressed by sorbitol) ------- COMMENT: c480fb02b530ca54 15 wjZExu1SPIyYHykVrnlpGn8ddvA Table 1 (comment: CHECK not suppressed by sorbitol) ------- COMMENT: c480fb02b530ca54 16 wjZExu1SPIyYHykVrnlpGn8ddvA Table 1 (comment: CHECK not suppressed by sorbitol) ------- COMMENT: c480fb02b530ca54 17 wjZExu1SPIyYHykVrnlpGn8ddvA Table 1 (comment: CHECK not suppressed by sorbitol) ------- COMMENT: c480fb02b530ca54 18 wjZExu1SPIyYHykVrnlpGn8ddvA Table 1 (comment: CHECK not suppressed by sorbitol) ------- COMMENT: c480fb02b530ca54 19 wjZExu1SPIyYHykVrnlpGn8ddvA Table 1 (comment: CHECK not suppressed by sorbitol) ------- COMMENT: c480fb02b530ca54 21 wjZExu1SPIyYHykVrnlpGn8ddvA Table 1 (comment: CHECK not suppressed by sorbitol) ------- COMMENT: c480fb02b530ca54 22 wjZExu1SPIyYHykVrnlpGn8ddvA Table 1 (comment: CHECK not suppressed by sorbitol) ------- COMMENT: c480fb02b530ca54 23 wjZExu1SPIyYHykVrnlpGn8ddvA Table 1 (comment: CHECK not suppressed by sorbitol) ------- COMMENT: c480fb02b530ca54 24 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: c480fb02b530ca54 25 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: c480fb02b530ca54 26 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: c480fb02b530ca54 27 yAIQKp85P0YUzNg+1Pbocjnj86k Table 1A ------- COMMENT: c480fb02b530ca54 28 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: c480fb02b530ca54 30 RM5SatjZKgoFqykfl4kzrPU/XhE Fig1A, Fig2A ------- COMMENT: c480fb02b530ca54 31 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: c480fb02b530ca54 32 UsvNF5BPARoKed+5EMrDc4/jHRs Fig 1A, Fig2A ------- COMMENT: c480fb02b530ca54 33 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: c480fb02b530ca54 34 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: c480fb02b530ca54 35 IEXOkiUWzOm9cj/LI9e0Pz8MkSI Fig2B ------- COMMENT: c480fb02b530ca54 36 EHtZgeedYTWGQcK+s4KyN60lQg4 Fig 2C ------- COMMENT: c480fb02b530ca54 37 EHtZgeedYTWGQcK+s4KyN60lQg4 Fig 2C ------- COMMENT: c480fb02b530ca54 38 gK1UCrrhyaRpx4U0GCLU044wz1U Fig 3 (comment: increased cell width compared to single mutants) ------- COMMENT: c480fb02b530ca54 39 gK1UCrrhyaRpx4U0GCLU044wz1U Fig 3 (comment: increased cell width compared to single mutants) ------- COMMENT: c480fb02b530ca54 40 80itsWtQOwEfqq6r8Ph9GUsUxCk Fig 3 (comment: cell width is wider than either of the single mutants) ------- COMMENT: c480fb02b530ca54 41 WuHT18VQNGEkep9aj7AZu3juLD0 Fig 3 (comment: CHECK no increase in cell width compared to single mutants) ------- COMMENT: c480fb02b530ca54 43 gsk2BYzDOMOr4aLxu4mhv3Hmc98 Figure 4A ------- COMMENT: c480fb02b530ca54 44 gsk2BYzDOMOr4aLxu4mhv3Hmc98 Figure 4A ------- COMMENT: c480fb02b530ca54 45 gsk2BYzDOMOr4aLxu4mhv3Hmc98 Figure 4A ------- COMMENT: c480fb02b530ca54 46 O5ChQshWo9SMl9ef8VwaxdF3zLk Fig4B ------- COMMENT: c480fb02b530ca54 47 O5ChQshWo9SMl9ef8VwaxdF3zLk Fig4B ------- COMMENT: c480fb02b530ca54 48 27QDPj8/0j89z1YQuMNwh3zOZws Fig4B, Fig8 (comment: localisation is actin dependent) ------- COMMENT: c480fb02b530ca54 49 27QDPj8/0j89z1YQuMNwh3zOZws Fig4B, Fig8 (comment: localisation is actin dependent) ------- COMMENT: c480fb02b530ca54 50 PXqQ8w7WZKoenOmS6L1/H8ouUb4 Fig4A ------- COMMENT: c480fb02b530ca54 51 WNrCsvDaF6+KKnINvKEtBA/avXU Fig4C ------- COMMENT: c480fb02b530ca54 52 Ozp47kvNh5k2BQB6BeY/MCsDsaY Fig4D ------- COMMENT: c480fb02b530ca54 53 IEXOkiUWzOm9cj/LI9e0Pz8MkSI Fig2B ------- COMMENT: c480fb02b530ca54 54 U1NiAwkwFveDm8425RQEx2O6HgY Fig5 ------- COMMENT: c480fb02b530ca54 55 U1NiAwkwFveDm8425RQEx2O6HgY Fig5 ------- COMMENT: c480fb02b530ca54 56 U1NiAwkwFveDm8425RQEx2O6HgY Fig5 ------- COMMENT: c480fb02b530ca54 57 17w3ooyAUKy+WF4dyjOeyUH3fZ4 Fig 6A (comment: CHECK fusion protein driven from nmt41 promoter) ------- COMMENT: c480fb02b530ca54 58 Ecq5Bcmh1sMm1c1uOPqR7p4sZpc Fig 6B (comment: CHECK fusion protein driven from nmt41 promoter) ------- COMMENT: c480fb02b530ca54 59 OXqCw37/Uyk9HcquOWmbm+UAQCU Fig6C ------- COMMENT: c480fb02b530ca54 60 OXqCw37/Uyk9HcquOWmbm+UAQCU Fig6C ------- COMMENT: c480fb02b530ca54 61 EKOwnXAhEp6lw44ywqqFINFUa50 Fig7A, B ------- COMMENT: c480fb02b530ca54 62 vewXoAnKYWVTkrnp7KCogyhZvfI Fig7A, B localisation of rga4 by blt1+ is more extensive than wild type rga4 localisation but rescues the wide cell phenotype of the rga4 deletion ------- COMMENT: c480fb02b530ca54 67 O9ZeGp02Viz4sP5dzksjpvcBNXU Fig9 (comment: cdc42-CRIB-GFP localisation is actin dependent and sensitive to low levels (10mM) Lat A) ------- COMMENT: c480fb02b530ca54 68 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: c480fb02b530ca54 69 wjZExu1SPIyYHykVrnlpGn8ddvA Table 1 (comment: CHECK not suppressed by sorbitol) ------- COMMENT: c480fb02b530ca54 70 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: c480fb02b530ca54 71 Ozp47kvNh5k2BQB6BeY/MCsDsaY Fig4D ------- COMMENT: c49a19fd6aa23bc4 1 1yzJJH5lydLDDcycP/lEvuZz4gM (comment: binds both circular and linear DNA fragments) ------- COMMENT: c49a19fd6aa23bc4 2 0u8KkeINmHCh6V6nUzxQrcOqn2Y (comment: CHECK determined as homodimeric by size exclusion chromatography) ------- COMMENT: c49a19fd6aa23bc4 3 XJYCKcjQKn6gW486f/eJLUQnXwg (comment: CHECK i) xlf1 deletion is epistatic with lig4 deletion ii) IR sensitivity during spore state and inability to ligate linearised plasmids during vegetative state) ------- COMMENT: c49f61a6f4aa2c82 1 cRz9RvwZJ+DOg6OU23DPEblfFv0 (comment: telomere southern (experiment)) ------- COMMENT: c49f61a6f4aa2c82 4 0Y9SZK5kgt0becgnr5Vs9ZtTGEQ (comment: CONDITION growth >48 hrs, growth to exponential phase) ------- COMMENT: c49f61a6f4aa2c82 5 50XZaaE5rpEuzOM9mlwqEfZKh+k (comment: CHECK lost in pof8D) ------- COMMENT: c49f61a6f4aa2c82 6 2z/fNPfn6Y1TZfL0+warNV42L7k (comment: CHECK lost in pof8D) / Bmc1 interacts with the full-length, 3’ end- matured TER1 associated with the active telomerase holoenzyme / Bmc1 interacts with the primary cohort of TMG-capped TER1 transcripts ------- COMMENT: c49f61a6f4aa2c82 8 TnZ0ywacjPSuJrIKMZUocgtaXOQ (comment: CHECK Lost in bmc1D) ------- COMMENT: c49f61a6f4aa2c82 9 XWwXDPoPJqe4oXkUZ+W9Vf+9cxY (comment: CHECK not lost when treated with benzonase or ter1D) ------- COMMENT: c49f61a6f4aa2c82 10 IGdBYmLPHLyIyTl12gINo24sqrE (comment: CHECK lost when treated with benzonase or ter1D) ------- COMMENT: c49f61a6f4aa2c82 13 cRz9RvwZJ+DOg6OU23DPEblfFv0 (comment: telomere southern (experiment)) ------- COMMENT: c49f61a6f4aa2c82 19 gGYV3NLuwVoyLLcZxkHhwl2oisI (comment: CHECK rescue of FYPO:0006849) ------- COMMENT: c49f61a6f4aa2c82 35 46Ojn2fAz1xTeDDy2rqWPh2H1hM (comment: CHECK partial rescue of FYPO:0006849) ------- COMMENT: c4b723fee955f9ee 1 77BX97Fy/WpQshPpHe6LX4ohkq4 (Fig. 1A and B) ------- COMMENT: c4f2687cd9a265cf 1 nJs97Pz0XCn23e/G3M721jPSY8o fig 2A, B ------- COMMENT: c4f2687cd9a265cf 2 nJs97Pz0XCn23e/G3M721jPSY8o fig 2A, B ------- COMMENT: c4f2687cd9a265cf 3 sCIdCFk+kzKh+/LlsgEbhnbxD5I fig 2C, D ------- COMMENT: c4f2687cd9a265cf 4 sCIdCFk+kzKh+/LlsgEbhnbxD5I fig 2C, D ------- COMMENT: c4f2687cd9a265cf 5 sCIdCFk+kzKh+/LlsgEbhnbxD5I fig 2C, D ------- COMMENT: c4f2687cd9a265cf 6 Dz94FH5ZqzxKYmwf52yBFtdtNOQ fig 2E ------- COMMENT: c4f2687cd9a265cf 7 Dz94FH5ZqzxKYmwf52yBFtdtNOQ fig 2E ------- COMMENT: c4f2687cd9a265cf 8 Dz94FH5ZqzxKYmwf52yBFtdtNOQ fig 2E ------- COMMENT: c4f2687cd9a265cf 9 3yZpotVk5MekLLV12WyROmZApE4 Fig.3A top ------- COMMENT: c4f2687cd9a265cf 10 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: c4f2687cd9a265cf 11 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: c4f2687cd9a265cf 12 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: c4f2687cd9a265cf 13 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: c4f2687cd9a265cf 14 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: c4f2687cd9a265cf 15 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: c4f2687cd9a265cf 17 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: c4f2687cd9a265cf 18 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: c4f2687cd9a265cf 19 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: c4f2687cd9a265cf 20 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: c4f2687cd9a265cf 21 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: c4f2687cd9a265cf 22 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: c4f2687cd9a265cf 23 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: c4f2687cd9a265cf 24 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: c4f2687cd9a265cf 25 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: c4f2687cd9a265cf 26 aORULJjF7t7Ol2ZudPyNwVB5r+E Figure 4A, B ------- COMMENT: c4f2687cd9a265cf 27 aORULJjF7t7Ol2ZudPyNwVB5r+E Figure 4A, B ------- COMMENT: c4f2687cd9a265cf 28 aORULJjF7t7Ol2ZudPyNwVB5r+E Figure 4A, B ------- COMMENT: c4f2687cd9a265cf 29 aORULJjF7t7Ol2ZudPyNwVB5r+E Figure 4A, B ------- COMMENT: c4f2687cd9a265cf 30 aORULJjF7t7Ol2ZudPyNwVB5r+E Figure 4A, B ------- COMMENT: c4f2687cd9a265cf 31 aORULJjF7t7Ol2ZudPyNwVB5r+E Figure 4A, B ------- COMMENT: c4f2687cd9a265cf 32 aORULJjF7t7Ol2ZudPyNwVB5r+E Figure 4A, B ------- COMMENT: c4f2687cd9a265cf 33 aORULJjF7t7Ol2ZudPyNwVB5r+E Figure 4A, B ------- COMMENT: c4f2687cd9a265cf 34 aORULJjF7t7Ol2ZudPyNwVB5r+E Figure 4A, B ------- COMMENT: c4f2687cd9a265cf 35 aORULJjF7t7Ol2ZudPyNwVB5r+E Figure 4A, B ------- COMMENT: c4f2687cd9a265cf 36 aORULJjF7t7Ol2ZudPyNwVB5r+E Figure 4A, B ------- COMMENT: c4f2687cd9a265cf 37 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: c4f2687cd9a265cf 38 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: c4f2687cd9a265cf 39 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: c4f2687cd9a265cf 40 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: c4f2687cd9a265cf 41 +PogSmkFjw+MDqGd0jejmPXUWMU Fig. 5B, C) ------- COMMENT: c4f2687cd9a265cf 42 +PogSmkFjw+MDqGd0jejmPXUWMU Fig. 5B, C) ------- COMMENT: c4f2687cd9a265cf 43 +PogSmkFjw+MDqGd0jejmPXUWMU Fig. 5B, C) ------- COMMENT: c4f2687cd9a265cf 44 +PogSmkFjw+MDqGd0jejmPXUWMU Fig. 5B, C) ------- COMMENT: c4f2687cd9a265cf 45 QeR961vJ1bS6XvPSsMtP1EMfa5g Fig. 5D and Supplementary Fig. S2A,C ------- COMMENT: c4f2687cd9a265cf 46 QeR961vJ1bS6XvPSsMtP1EMfa5g Fig. 5D and Supplementary Fig. S2A,C ------- COMMENT: c4f2687cd9a265cf 47 QeR961vJ1bS6XvPSsMtP1EMfa5g Fig. 5D and Supplementary Fig. S2A,C ------- COMMENT: c4f2687cd9a265cf 48 QeR961vJ1bS6XvPSsMtP1EMfa5g Fig. 5D and Supplementary Fig. S2A,C ------- COMMENT: c4f2687cd9a265cf 49 0JtWWbGerTmkzK8lAu6rlAYe7tU Figure 5E ------- COMMENT: c4f2687cd9a265cf 50 0JtWWbGerTmkzK8lAu6rlAYe7tU Figure 5E ------- COMMENT: c4f2687cd9a265cf 51 0JtWWbGerTmkzK8lAu6rlAYe7tU Figure 5E ------- COMMENT: c4f2687cd9a265cf 52 0JtWWbGerTmkzK8lAu6rlAYe7tU Figure 5E ------- COMMENT: c4f2687cd9a265cf 53 xMb+ceyiL6+pd7TnS6nh6L8CKcI Figure 6C-E ------- COMMENT: c4f2687cd9a265cf 54 xMb+ceyiL6+pd7TnS6nh6L8CKcI Figure 6C-E ------- COMMENT: c4f2687cd9a265cf 55 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: c4f2687cd9a265cf 56 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: c4f2687cd9a265cf 57 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: c4f2687cd9a265cf 58 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: c52a25b43bc64269 1 EoTSeVT5M4ryPAnn259w0JFoLVE (comment: CONDITION YES containing hygromycin B and galactose) ------- COMMENT: c52a25b43bc64269 2 EDfJ21bgVNmX1/kK6C7WN8z/ZqE (comment: galactosylation defect) ------- COMMENT: c52a25b43bc64269 3 EDfJ21bgVNmX1/kK6C7WN8z/ZqE (comment: galactosylation defect) ------- COMMENT: c52a25b43bc64269 4 4I5IJlugkxczZdwex2EyfAHwiaw (comment: CHECK complemented by S. cerevisiae GAL2) ------- COMMENT: c52a25b43bc64269 6 sBwXSfJgtMXnVvlBgx4IQ/40VPY FIgure 2. (comment: CONDITION +25 μg/ml Hyg.B) ------- COMMENT: c52a25b43bc64269 7 zAo7t3OHgUxgq/mX7siYcq0Ew3E FIgure 2 ------- COMMENT: c52a25b43bc64269 8 zAo7t3OHgUxgq/mX7siYcq0Ew3E FIgure 2 ------- COMMENT: c52a25b43bc64269 9 zAo7t3OHgUxgq/mX7siYcq0Ew3E FIgure 2 ------- COMMENT: c52a25b43bc64269 10 zAo7t3OHgUxgq/mX7siYcq0Ew3E FIgure 2 ------- COMMENT: c52a25b43bc64269 11 zAo7t3OHgUxgq/mX7siYcq0Ew3E FIgure 2 ------- COMMENT: c52a25b43bc64269 12 zAo7t3OHgUxgq/mX7siYcq0Ew3E FIgure 2 ------- COMMENT: c52a25b43bc64269 13 zAo7t3OHgUxgq/mX7siYcq0Ew3E FIgure 2 ------- COMMENT: c52a25b43bc64269 14 zAo7t3OHgUxgq/mX7siYcq0Ew3E FIgure 2 ------- COMMENT: c52a25b43bc64269 15 c5qb/r1D6y9nzjQRKSIrjbVy0dg FIgure 2. ------- COMMENT: c52a25b43bc64269 16 c5qb/r1D6y9nzjQRKSIrjbVy0dg FIgure 2. ------- COMMENT: c52a25b43bc64269 17 c5qb/r1D6y9nzjQRKSIrjbVy0dg FIgure 2. ------- COMMENT: c52a25b43bc64269 18 c5qb/r1D6y9nzjQRKSIrjbVy0dg FIgure 2. ------- COMMENT: c52a25b43bc64269 19 c5qb/r1D6y9nzjQRKSIrjbVy0dg FIgure 2. ------- COMMENT: c52a25b43bc64269 20 c5qb/r1D6y9nzjQRKSIrjbVy0dg FIgure 2. ------- COMMENT: c52a25b43bc64269 21 c5qb/r1D6y9nzjQRKSIrjbVy0dg FIgure 2. ------- COMMENT: c52a25b43bc64269 22 c5qb/r1D6y9nzjQRKSIrjbVy0dg FIgure 2. ------- COMMENT: c52a25b43bc64269 23 c5qb/r1D6y9nzjQRKSIrjbVy0dg FIgure 2. ------- COMMENT: c52a25b43bc64269 24 sBwXSfJgtMXnVvlBgx4IQ/40VPY FIgure 2. (comment: CONDITION +25 μg/ml Hyg.B) ------- COMMENT: c52a25b43bc64269 25 sBwXSfJgtMXnVvlBgx4IQ/40VPY FIgure 2. (comment: CONDITION +25 μg/ml Hyg.B) ------- COMMENT: c52a25b43bc64269 26 sBwXSfJgtMXnVvlBgx4IQ/40VPY FIgure 2. (comment: CONDITION +25 μg/ml Hyg.B) ------- COMMENT: c52a25b43bc64269 27 sBwXSfJgtMXnVvlBgx4IQ/40VPY FIgure 2. (comment: CONDITION +25 μg/ml Hyg.B) ------- COMMENT: c52a25b43bc64269 28 sBwXSfJgtMXnVvlBgx4IQ/40VPY FIgure 2. (comment: CONDITION +25 μg/ml Hyg.B) ------- COMMENT: c52a25b43bc64269 29 sBwXSfJgtMXnVvlBgx4IQ/40VPY FIgure 2. (comment: CONDITION +25 μg/ml Hyg.B) ------- COMMENT: c52a25b43bc64269 30 sBwXSfJgtMXnVvlBgx4IQ/40VPY FIgure 2. (comment: CONDITION +25 μg/ml Hyg.B) ------- COMMENT: c52a25b43bc64269 31 sBwXSfJgtMXnVvlBgx4IQ/40VPY FIgure 2. (comment: CONDITION +25 μg/ml Hyg.B) ------- COMMENT: c52a25b43bc64269 32 sBwXSfJgtMXnVvlBgx4IQ/40VPY FIgure 2. (comment: CONDITION +25 μg/ml Hyg.B) ------- COMMENT: c52a25b43bc64269 33 13He6/SGGFDCdHpxT3yTqdll83A Fig 3. galactose-specific HRP-PNA staining was used to detect quantitative differences in the galactosyla- tion of cell-surface proteins ------- COMMENT: c52a25b43bc64269 34 13He6/SGGFDCdHpxT3yTqdll83A Fig 3. galactose-specific HRP-PNA staining was used to detect quantitative differences in the galactosyla- tion of cell-surface proteins ------- COMMENT: c52a25b43bc64269 35 XnOOYeY/WgdHnVmmmZbkO0OJeCo fig 5a There was no difference in ght2+ expression levels between wild-type and uge1Δgal10Δ cells that have a reduced level of cytosolic UDP-galactose (Suzuki et al. 2010), indicating that expression of ght2+ is not influenced by intracellular UDP-galactose concentration ------- COMMENT: c52a25b43bc64269 36 XnOOYeY/WgdHnVmmmZbkO0OJeCo fig 5a There was no difference in ght2+ expression levels between wild-type and uge1Δgal10Δ cells that have a reduced level of cytosolic UDP-galactose (Suzuki et al. 2010), indicating that expression of ght2+ is not influenced by intracellular UDP-galactose concentration ------- COMMENT: c52a25b43bc64269 37 XnOOYeY/WgdHnVmmmZbkO0OJeCo fig 5a There was no difference in ght2+ expression levels between wild-type and uge1Δgal10Δ cells that have a reduced level of cytosolic UDP-galactose (Suzuki et al. 2010), indicating that expression of ght2+ is not influenced by intracellular UDP-galactose concentration ------- COMMENT: c56a652b8b068e66 2 68p7wDLFLRdwvv/DD8pXblqy7K4 Similarly, both nup132Δ and pli1Δ cells showed sensitivity to the microtubule-stabilising drug thiabendazole (TBZ), consistent with defects in centromere function, and this TBZ sensitivity was also not rescued by the Pli1K3R mutation (Fig. 1C). T ------- COMMENT: c56a652b8b068e66 3 68p7wDLFLRdwvv/DD8pXblqy7K4 Similarly, both nup132Δ and pli1Δ cells showed sensitivity to the microtubule-stabilising drug thiabendazole (TBZ), consistent with defects in centromere function, and this TBZ sensitivity was also not rescued by the Pli1K3R mutation (Fig. 1C). T ------- COMMENT: c56a652b8b068e66 4 68p7wDLFLRdwvv/DD8pXblqy7K4 Similarly, both nup132Δ and pli1Δ cells showed sensitivity to the microtubule-stabilising drug thiabendazole (TBZ), consistent with defects in centromere function, and this TBZ sensitivity was also not rescued by the Pli1K3R mutation (Fig. 1C). T ------- COMMENT: c56a652b8b068e66 5 68p7wDLFLRdwvv/DD8pXblqy7K4 Similarly, both nup132Δ and pli1Δ cells showed sensitivity to the microtubule-stabilising drug thiabendazole (TBZ), consistent with defects in centromere function, and this TBZ sensitivity was also not rescued by the Pli1K3R mutation (Fig. 1C). T ------- COMMENT: c56a652b8b068e66 6 qQL1Bj/kOMiVWPJFM7EatDeuttc If the silencing defect in nup132Δ cells were due to destabilisation of Pli1, we would expect it to be rescued by expression of the stabilised Pli1K3R mutant. However, this was not the case – nup132Δ cells expressing Pli1K3R–Flag displayed a defect in silencing equivalent to those expressing wild-type Pli1–Flag ------- COMMENT: c56a652b8b068e66 7 Jzz9xtIRjQVkkER/m3qO5LTY0DU , deletion of either nup132+ or pli1+ results in reduced growth in the presence of 5-FOA, indicating increased expression of ura4+ and hence loss of silencing (Fig. 1C). ------- COMMENT: c56a652b8b068e66 8 Jzz9xtIRjQVkkER/m3qO5LTY0DU , deletion of either nup132+ or pli1+ results in reduced growth in the presence of 5-FOA, indicating increased expression of ura4+ and hence loss of silencing (Fig. 1C). ------- COMMENT: c56a652b8b068e66 10 2rXgkWrr9cr2xu6J4/LkYNjdurA However, in nup132Δ cells, whereas wild-type Pli1 was destabilised, Pli1K3R levels remained high (Fig. 1B). ------- COMMENT: c56a652b8b068e66 11 2rXgkWrr9cr2xu6J4/LkYNjdurA However, in nup132Δ cells, whereas wild-type Pli1 was destabilised, Pli1K3R levels remained high (Fig. 1B). ------- COMMENT: c56a652b8b068e66 14 8zdno63XFs3BFsVP161LRtfCMNM Figure 3D ------- COMMENT: c56a652b8b068e66 73 CXtsFfxBGGjIy2XS5Aym6EPTC9k (comment: CHECK inhibits) ------- COMMENT: c56a652b8b068e66 74 CXtsFfxBGGjIy2XS5Aym6EPTC9k (comment: CHECK inhibits) ------- COMMENT: c578121eb386a5c8 17 lznybir1DAL51vgYisyc4wHUgcg (comment: beta tubulin specific pathway) ------- COMMENT: c57dbb2608ee460d 1 Bt+IdkDLJzq3GUJaO7M84t9JWhA (comment: CONDITION 25ºC) ------- COMMENT: c57dbb2608ee460d 2 Bt+IdkDLJzq3GUJaO7M84t9JWhA (comment: CONDITION 25ºC) ------- COMMENT: c57dbb2608ee460d 3 Bt+IdkDLJzq3GUJaO7M84t9JWhA (comment: CONDITION 25ºC) ------- COMMENT: c57dbb2608ee460d 4 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 6 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 7 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 8 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 9 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 10 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 11 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 12 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 13 0Ij3zhRvZpbii0cGYusw2KWJDsY (comment: 25ºC, live-cell imaging, cell length at septation) ------- COMMENT: c57dbb2608ee460d 14 0Ij3zhRvZpbii0cGYusw2KWJDsY (comment: 25ºC, live-cell imaging, cell length at septation) ------- COMMENT: c57dbb2608ee460d 15 Bt+IdkDLJzq3GUJaO7M84t9JWhA (comment: CONDITION 25ºC) ------- COMMENT: c57dbb2608ee460d 16 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 17 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 18 Bt+IdkDLJzq3GUJaO7M84t9JWhA (comment: CONDITION 25ºC) ------- COMMENT: c57dbb2608ee460d 19 Bt+IdkDLJzq3GUJaO7M84t9JWhA (comment: CONDITION 25ºC) ------- COMMENT: c57dbb2608ee460d 20 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 21 1Tba1qo6uZwdZiF0EC7h/1JFYCo (comment: CONDITION 32ºC) ------- COMMENT: c57dbb2608ee460d 22 1Tba1qo6uZwdZiF0EC7h/1JFYCo (comment: CONDITION 32ºC) ------- COMMENT: c57dbb2608ee460d 23 1Tba1qo6uZwdZiF0EC7h/1JFYCo (comment: CONDITION 32ºC) ------- COMMENT: c57dbb2608ee460d 24 1Tba1qo6uZwdZiF0EC7h/1JFYCo (comment: CONDITION 32ºC) ------- COMMENT: c57dbb2608ee460d 25 1Tba1qo6uZwdZiF0EC7h/1JFYCo (comment: CONDITION 32ºC) ------- COMMENT: c57dbb2608ee460d 26 1Tba1qo6uZwdZiF0EC7h/1JFYCo (comment: CONDITION 32ºC) ------- COMMENT: c57dbb2608ee460d 27 1Tba1qo6uZwdZiF0EC7h/1JFYCo (comment: CONDITION 32ºC) ------- COMMENT: c57dbb2608ee460d 28 1Tba1qo6uZwdZiF0EC7h/1JFYCo (comment: CONDITION 32ºC) ------- COMMENT: c57dbb2608ee460d 29 1Tba1qo6uZwdZiF0EC7h/1JFYCo (comment: CONDITION 32ºC) ------- COMMENT: c57dbb2608ee460d 36 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 37 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 38 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 39 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 40 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 41 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 42 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 43 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 44 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 45 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 46 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 47 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 48 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 49 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 50 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 51 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 52 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 53 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 54 Bt+IdkDLJzq3GUJaO7M84t9JWhA (comment: CONDITION 25ºC) ------- COMMENT: c57dbb2608ee460d 55 Bt+IdkDLJzq3GUJaO7M84t9JWhA (comment: CONDITION 25ºC) ------- COMMENT: c57dbb2608ee460d 56 Bt+IdkDLJzq3GUJaO7M84t9JWhA (comment: CONDITION 25ºC) ------- COMMENT: c57dbb2608ee460d 57 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57dbb2608ee460d 58 b0MBdoEbf5s/y06CJC8bLcGZ+Ps (comment: live-cell imaging, 25ºC) ------- COMMENT: c57e1d4a37ec567b 7 B1OjZFz12XO89v/Obyy/dxAcox4 (comment: FLAG-Ago1) ------- COMMENT: c57e1d4a37ec567b 8 VepI+o8b1TkshWfDlZOFXzmp2Oc (comment: Myc-Ago1) ------- COMMENT: c57e1d4a37ec567b 15 VepI+o8b1TkshWfDlZOFXzmp2Oc (comment: Myc-Ago1) ------- COMMENT: c57e1d4a37ec567b 16 B1OjZFz12XO89v/Obyy/dxAcox4 (comment: FLAG-Ago1) ------- COMMENT: c57e1d4a37ec567b 17 aJpJEV6si3khFQQsUTFF0QT+q88 (comment: FLAG-Ago1, Arb1-Myc) ------- COMMENT: c57e1d4a37ec567b 18 aJpJEV6si3khFQQsUTFF0QT+q88 (comment: FLAG-Ago1, Arb1-Myc) ------- COMMENT: c57e1d4a37ec567b 19 x7eW2vBJ6Z2h7MlDhManA1POoZo (comment: Tas3-Myc) ------- COMMENT: c599c5b1b32b1bc9 1 ljVcY8/buZu7wpDceWvmv4FMzns Fig. 1c, Supplementary Fig. 2a ------- COMMENT: c599c5b1b32b1bc9 2 4a9wdVyIUx6/j/hL98AQAJQsh5k Fig 1a, b ------- COMMENT: c599c5b1b32b1bc9 3 4a9wdVyIUx6/j/hL98AQAJQsh5k Fig 1a, b ------- COMMENT: c599c5b1b32b1bc9 4 4a9wdVyIUx6/j/hL98AQAJQsh5k Fig 1a, b ------- COMMENT: c599c5b1b32b1bc9 8 y+Bd7x/dmx4IJoyT99V0/j/M48w Fig. 2a, Supplementary Fig. 3a ------- COMMENT: c599c5b1b32b1bc9 9 y+Bd7x/dmx4IJoyT99V0/j/M48w Fig. 2a, Supplementary Fig. 3a ------- COMMENT: c599c5b1b32b1bc9 10 y+Bd7x/dmx4IJoyT99V0/j/M48w Fig. 2a, Supplementary Fig. 3a ------- COMMENT: c599c5b1b32b1bc9 11 y+Bd7x/dmx4IJoyT99V0/j/M48w Fig. 2a, Supplementary Fig. 3a ------- COMMENT: c599c5b1b32b1bc9 12 y+Bd7x/dmx4IJoyT99V0/j/M48w Fig. 2a, Supplementary Fig. 3a ------- COMMENT: c599c5b1b32b1bc9 13 y+Bd7x/dmx4IJoyT99V0/j/M48w Fig. 2a, Supplementary Fig. 3a ------- COMMENT: c599c5b1b32b1bc9 14 oKBerele0zfDMzatmcDfvUlNtjA Supplementary Table 1 ------- COMMENT: c599c5b1b32b1bc9 16 idwTye6F2Hxyh9LiCtdXzdeqaIg Fig. 2c, d, e ------- COMMENT: c599c5b1b32b1bc9 17 idwTye6F2Hxyh9LiCtdXzdeqaIg Fig. 2c, d, e ------- COMMENT: c599c5b1b32b1bc9 18 idwTye6F2Hxyh9LiCtdXzdeqaIg Fig. 2c, d, e ------- COMMENT: c599c5b1b32b1bc9 19 y+Bd7x/dmx4IJoyT99V0/j/M48w Fig. 2a, Supplementary Fig. 3a ------- COMMENT: c599c5b1b32b1bc9 20 y+Bd7x/dmx4IJoyT99V0/j/M48w Fig. 2a, Supplementary Fig. 3a ------- COMMENT: c599c5b1b32b1bc9 21 y+Bd7x/dmx4IJoyT99V0/j/M48w Fig. 2a, Supplementary Fig. 3a ------- COMMENT: c599c5b1b32b1bc9 22 aXJLEb5auIthKy1HbJeCPFxuqBU Supplementary Fig. 6 ------- COMMENT: c599c5b1b32b1bc9 23 aXJLEb5auIthKy1HbJeCPFxuqBU Supplementary Fig. 6 ------- COMMENT: c599c5b1b32b1bc9 30 HzQO9biP7ipvXNPhPBifJYcF+mk Fig. 3a ------- COMMENT: c599c5b1b32b1bc9 31 y+Bd7x/dmx4IJoyT99V0/j/M48w Fig. 2a, Supplementary Fig. 3a ------- COMMENT: c599c5b1b32b1bc9 32 y+Bd7x/dmx4IJoyT99V0/j/M48w Fig. 2a, Supplementary Fig. 3a ------- COMMENT: c599c5b1b32b1bc9 34 y+Bd7x/dmx4IJoyT99V0/j/M48w Fig. 2a, Supplementary Fig. 3a ------- COMMENT: c599c5b1b32b1bc9 35 y+Bd7x/dmx4IJoyT99V0/j/M48w Fig. 2a, Supplementary Fig. 3a ------- COMMENT: c599c5b1b32b1bc9 38 y+Bd7x/dmx4IJoyT99V0/j/M48w Fig. 2a, Supplementary Fig. 3a ------- COMMENT: c599c5b1b32b1bc9 39 y+Bd7x/dmx4IJoyT99V0/j/M48w Fig. 2a, Supplementary Fig. 3a ------- COMMENT: c599c5b1b32b1bc9 40 WosERFUQbEyLdhwC/c5gUkGPz6Q Supplementary Figure S8 ------- COMMENT: c599c5b1b32b1bc9 41 WosERFUQbEyLdhwC/c5gUkGPz6Q Supplementary Figure S8 ------- COMMENT: c599c5b1b32b1bc9 42 WosERFUQbEyLdhwC/c5gUkGPz6Q Supplementary Figure S8 ------- COMMENT: c599c5b1b32b1bc9 43 WosERFUQbEyLdhwC/c5gUkGPz6Q Supplementary Figure S8 ------- COMMENT: c599c5b1b32b1bc9 44 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: c599c5b1b32b1bc9 45 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: c599c5b1b32b1bc9 47 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: c599c5b1b32b1bc9 48 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: c599c5b1b32b1bc9 49 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: c599c5b1b32b1bc9 50 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: c599c5b1b32b1bc9 51 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: c599c5b1b32b1bc9 52 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: c599c5b1b32b1bc9 53 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: c599c5b1b32b1bc9 54 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: c599c5b1b32b1bc9 55 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: c599c5b1b32b1bc9 56 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: c599c5b1b32b1bc9 57 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: c599c5b1b32b1bc9 58 6icJ8C6RjJqGD2CHIzeWbgIvD9o Supplementary Fig. 10 ------- COMMENT: c599c5b1b32b1bc9 59 cDD/nHq1SjcRDonTC0XBAiM4YAk all data These data indi- cate that Pom1 functions in a dose-dependent manner to delay entry into mitosis through negative regulation of the Cdr2-Cdr1-Wee1 pathway. The pom1D size phenotype is not as severe as wee11 deletion, indicating that further Wee1 regulatory mechanisms are likely to be operating. We conclude that Pom1 is a potential functional link between polarized cell growth and mitotic entry by regulating these two processes. ------- COMMENT: c599c5b1b32b1bc9 60 cDD/nHq1SjcRDonTC0XBAiM4YAk all data These data indi- cate that Pom1 functions in a dose-dependent manner to delay entry into mitosis through negative regulation of the Cdr2-Cdr1-Wee1 pathway. The pom1D size phenotype is not as severe as wee11 deletion, indicating that further Wee1 regulatory mechanisms are likely to be operating. We conclude that Pom1 is a potential functional link between polarized cell growth and mitotic entry by regulating these two processes. ------- COMMENT: c599c5b1b32b1bc9 61 cDD/nHq1SjcRDonTC0XBAiM4YAk all data These data indi- cate that Pom1 functions in a dose-dependent manner to delay entry into mitosis through negative regulation of the Cdr2-Cdr1-Wee1 pathway. The pom1D size phenotype is not as severe as wee11 deletion, indicating that further Wee1 regulatory mechanisms are likely to be operating. We conclude that Pom1 is a potential functional link between polarized cell growth and mitotic entry by regulating these two processes. ------- COMMENT: c599c5b1b32b1bc9 62 kJdBShbPgRujByGXiy/ij+T7u9w Supplementary Table 2 ------- COMMENT: c599c5b1b32b1bc9 63 8Fbvw1rAenzNMGlGY4OOJ9Lan7k Supplementary Table 2 These experiments support the pom1 gradient model, pom1 is delocalized in tea1 delete ------- COMMENT: c599c5b1b32b1bc9 64 8Fbvw1rAenzNMGlGY4OOJ9Lan7k Supplementary Table 2 These experiments support the pom1 gradient model, pom1 is delocalized in tea1 delete ------- COMMENT: c599c5b1b32b1bc9 65 8Fbvw1rAenzNMGlGY4OOJ9Lan7k Supplementary Table 2 These experiments support the pom1 gradient model, pom1 is delocalized in tea1 delete ------- COMMENT: c599c5b1b32b1bc9 66 GiVS8KF4cEzKgy28VU9kY46cVBU Figure 4d ------- COMMENT: c599c5b1b32b1bc9 67 cDD/nHq1SjcRDonTC0XBAiM4YAk all data These data indi- cate that Pom1 functions in a dose-dependent manner to delay entry into mitosis through negative regulation of the Cdr2-Cdr1-Wee1 pathway. The pom1D size phenotype is not as severe as wee11 deletion, indicating that further Wee1 regulatory mechanisms are likely to be operating. We conclude that Pom1 is a potential functional link between polarized cell growth and mitotic entry by regulating these two processes. ------- COMMENT: c5b0c32f69a1468d 1 W4xkP4H/sC1fm9WHOucBJfKfR7Y (Fig. 2) ------- COMMENT: c5b0c32f69a1468d 2 W4xkP4H/sC1fm9WHOucBJfKfR7Y (Fig. 2) ------- COMMENT: c5b0c32f69a1468d 3 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: c5b0c32f69a1468d 4 UgxlI7ceYBHjVortzQjXraCa1Ng (Fig. 4I and K) ------- COMMENT: c5b0c32f69a1468d 5 GkI93EYSqP6NybAtsJjxO2fy3Ic (Fig. 4D) ------- COMMENT: c5b0c32f69a1468d 6 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: c5b0c32f69a1468d 10 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: c5b0c32f69a1468d 11 UgxlI7ceYBHjVortzQjXraCa1Ng (Fig. 4I and K) ------- COMMENT: c5b0c32f69a1468d 12 /WWsy5Fid9XMFh2nOHtFj2aZYss (Fig. 4J) ------- COMMENT: c5b0c32f69a1468d 13 /WWsy5Fid9XMFh2nOHtFj2aZYss (Fig. 4J) ------- COMMENT: c5b0c32f69a1468d 14 gS/dfmL8tgkBYixriL3fHMfmSLA These results suggest that Ntp1-mediated trehalose degra­dation is required for cytoplasmic fluidization and rapid germi­ nation through the cAMP-PKA pathway. ------- COMMENT: c5b0c32f69a1468d 15 AqhzSZS7KIkrbbcK5CpphNisDaI These results suggest that Ntp1-mediated trehalose degra­dation is required for cytoplasmic fluidization and rapid germi­nation through the cAMP-PKA pathway. ------- COMMENT: c5c033ad2e33e885 12 p7XN1Z8xzPKIeYzDYbCvGRRtTJA Checkpoint deficiency in the presence of doxorubicin of aph1delta enhanced by cds1 hypomorphic allele ------- COMMENT: c5c033ad2e33e885 13 Q/gzNI5WHOrphfqs84Hkd+uyEQQ Mutant proliferates faster and with shorter lag than wildtype in sublethal concentrations of hydroxyurea, phleomycin or doxorubicin ------- COMMENT: c5c033ad2e33e885 14 Q/gzNI5WHOrphfqs84Hkd+uyEQQ Mutant proliferates faster and with shorter lag than wildtype in sublethal concentrations of hydroxyurea, phleomycin or doxorubicin ------- COMMENT: c5c033ad2e33e885 15 Q/gzNI5WHOrphfqs84Hkd+uyEQQ Mutant proliferates faster and with shorter lag than wildtype in sublethal concentrations of hydroxyurea, phleomycin or doxorubicin ------- COMMENT: c5face93b651d68a 1 wsUR0ZccDsrMtPzuMVz0nYr86Kc fig 1A I'm modelling this as decreased because nda3 is providing microtubule damage. Checkpoint would be expected to be o in WT in this scenario. cells failed to maintain the microtubule damage-induced checkpoint arrest and began to aberrantly form septa ------- COMMENT: c5face93b651d68a 2 Khfr3uuiu4ZDnyHcjW86qrszdWc fig 1A I'm modelling this as normal becasue nda3 is providing microtubule damage. Checkpoint would be expected to be o in WT in this scenario. cells failed to maintain the microtubule damage-induced checkpoint arrest and began to aberrantly form septa ------- COMMENT: c5face93b651d68a 3 5vZgkEYP5KNK0cJX1cDP1m4YkEo The observation that Clp1p/Flp1p is required for sep- tation in dma1􏰋 mutants is consistent with a model where Dma1p inhibits SIN activation ------- COMMENT: c5face93b651d68a 4 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: c5face93b651d68a 5 vzlamEwW+KsplQL5zdHCdpilpCA Figure 2A, panels 11 and 14 ------- COMMENT: c5face93b651d68a 6 vzlamEwW+KsplQL5zdHCdpilpCA Figure 2A, panels 11 and 14 ------- COMMENT: c5face93b651d68a 7 Mc1Dl/8ePjG+W3BQIevC1vaEvqw Figure 2D ------- COMMENT: c5face93b651d68a 8 DDnTNAOfTVU1oIJ++9p0YR9GO7M (comment: faintly) Figure 3A, panel 2, arrowhead ------- COMMENT: c5face93b651d68a 9 iAKWJg760slbJv17HGL0TfPyQUs Figure 3A, panel 3 ------- COMMENT: c5face93b651d68a 10 iAKWJg760slbJv17HGL0TfPyQUs Figure 3A, panel 3 ------- COMMENT: c5face93b651d68a 11 ywppzxnIVeqi9tuvMHmOX8k8vTA Figures 3E, 3F ------- COMMENT: c5face93b651d68a 12 ywppzxnIVeqi9tuvMHmOX8k8vTA Figures 3E, 3F ------- COMMENT: c5face93b651d68a 13 G6dLbfUp9QIs4N8ht47nPA2YlLk Figures 3E, 3F) ------- COMMENT: c5face93b651d68a 14 G6dLbfUp9QIs4N8ht47nPA2YlLk Figures 3E, 3F) ------- COMMENT: c5face93b651d68a 15 G6dLbfUp9QIs4N8ht47nPA2YlLk Figures 3E, 3F) ------- COMMENT: c5face93b651d68a 16 G6dLbfUp9QIs4N8ht47nPA2YlLk Figures 3E, 3F) ------- COMMENT: c5face93b651d68a 17 G6dLbfUp9QIs4N8ht47nPA2YlLk Figures 3E, 3F) ------- COMMENT: c5face93b651d68a 18 G6dLbfUp9QIs4N8ht47nPA2YlLk Figures 3E, 3F) ------- COMMENT: c5face93b651d68a 19 G6dLbfUp9QIs4N8ht47nPA2YlLk Figures 3E, 3F) ------- COMMENT: c5face93b651d68a 20 kfOIBv5xxjvKi18aB6TtWiSeR0M Figure 4A (comment: CHECK 10% (2/20) of anaphase cells displayed Dma1p-GFP SPB signal) ------- COMMENT: c5face93b651d68a 21 hI5t2isRnTMyTI6yWT985lhWG+s Table1 ------- COMMENT: c5face93b651d68a 22 hI5t2isRnTMyTI6yWT985lhWG+s Table1 ------- COMMENT: c5face93b651d68a 23 hI5t2isRnTMyTI6yWT985lhWG+s Table1 ------- COMMENT: c5face93b651d68a 24 hI5t2isRnTMyTI6yWT985lhWG+s Table1 ------- COMMENT: c5face93b651d68a 25 hI5t2isRnTMyTI6yWT985lhWG+s Table1 ------- COMMENT: c5face93b651d68a 26 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: c5face93b651d68a 28 lfgp4+M8/jqsUopSdZrrCvOycXs Figure 4B) ------- COMMENT: c5face93b651d68a 29 LuCbZRmDWUgoXozgTLwGoQLTlwY Figure 4C, bottom ------- COMMENT: c5face93b651d68a 30 LuCbZRmDWUgoXozgTLwGoQLTlwY Figure 4C, bottom ------- COMMENT: c5face93b651d68a 31 h09KQpN7u/q6m3yDchMr2e10lc8 Fig4 ------- COMMENT: c5face93b651d68a 32 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: c5face93b651d68a 33 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: c5face93b651d68a 34 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: c5face93b651d68a 35 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: c5face93b651d68a 36 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: c5face93b651d68a 37 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: c605477efc21b69a 2 MHZTQrtI2W3A7aChbPgGbeOTSAs (Figure 4, S4B). ------- COMMENT: c605477efc21b69a 4 MHZTQrtI2W3A7aChbPgGbeOTSAs (Figure 4, S4B). ------- COMMENT: c605477efc21b69a 5 MHZTQrtI2W3A7aChbPgGbeOTSAs (Figure 4, S4B). ------- COMMENT: c605477efc21b69a 6 MHZTQrtI2W3A7aChbPgGbeOTSAs (Figure 4, S4B). ------- COMMENT: c605477efc21b69a 7 zDsD+3G2Ft9T7qz0dog8QAK01OI decreased Pil1 protein abundance Figure S1F ------- COMMENT: c605477efc21b69a 8 2uY7rUe5RJiyswekWVKTarheVLk Figure S3D) ------- COMMENT: c605477efc21b69a 9 v9s1wgLyF0FrCnBMYYBo3IAdoGY (Figure S1F). Pil1 mis-assembled into fewer and longer filaments ------- COMMENT: c605477efc21b69a 10 WIhSIYRm6cXhSDGyP/bBoOo0CiE fig2 the cortical tubular ER pattern changes faster than wild type ------- COMMENT: c605477efc21b69a 11 AHmgIeHvMQqBTTGVnW/aJ8OnRwg fig2 increased cortical ER remodeling dynamics ------- COMMENT: c605477efc21b69a 12 AHmgIeHvMQqBTTGVnW/aJ8OnRwg fig2 increased cortical ER remodeling dynamics ------- COMMENT: c605477efc21b69a 13 apqxrQe6f//hIUYUvwP2r5FsQcw (Fig- ure S5G). the cortical tubular ER pattern changes faster than wild type ------- COMMENT: c605477efc21b69a 14 JubmMq7j21JL8TPd5IoE4bE9NTM (Figure 3A) the cortical tubular ER pattern changes slower than wild type ------- COMMENT: c605477efc21b69a 15 6DW8WWt5IqObZ0lkOZ+vV64u5GM (Figure S3C). the cortical tubular ER pattern changes slower than wild type ------- COMMENT: c605477efc21b69a 18 B5Rh6/9unYjc6DSK2+aDXT5bbs0 (Figure 3A) decreased cortical ER remodeling dynamics the cortical tubular ER pattern changes slower than wild type ------- COMMENT: c605477efc21b69a 19 Qh4eas3coZv9ufPjL1AjALbQXLE the cortical tubular ER pattern changes slower than wild type ------- COMMENT: c605477efc21b69a 20 UdCivy3GSIRLwbylIfSVuKqvO4Y increased cortical ER remodeling dynamics ------- COMMENT: c605477efc21b69a 21 UdCivy3GSIRLwbylIfSVuKqvO4Y increased cortical ER remodeling dynamics ------- COMMENT: c605477efc21b69a 22 8uTuHIGp5KOzWuXHpjw5FwbzsbA increased cortical ER remodeling dynamics the cortical tubular ER pattern changes faster than wild type ------- COMMENT: c605477efc21b69a 26 Qh4eas3coZv9ufPjL1AjALbQXLE the cortical tubular ER pattern changes slower than wild type ------- COMMENT: c605477efc21b69a 27 Qh4eas3coZv9ufPjL1AjALbQXLE the cortical tubular ER pattern changes slower than wild type ------- COMMENT: c605477efc21b69a 28 sdEycnESOPJJ4WdfA2mmK+yappY (Figures 1A, 1B) (EM) data also confirmed that eisosomes/MCC associated with the cER, especially with curved cER rims, over the lateral cell cortex in WT (Figures 1C and S1A). Such an asso- ciation was abolished in scs2Dscs22D cells lacking ER-PM con- tacts.(comment: the Exp says more but I don't know how to capture that) ------- COMMENT: c605477efc21b69a 29 zW+UqahVTer/xUnP5n9zAC3OaPA (comment: PMID:32023460) Of note, the PM coverage of eisosomes was also reduced in scs2Dscs22D cells (Figure S1B), implicating VAPs in the regulation of eisosome assembly. ------- COMMENT: c605477efc21b69a 30 XbPrOSEWIS9vx5XA6KP1Od9b018 (Figure S1F). increased Pil1 phosphorylation was detected in these cells ------- COMMENT: c605477efc21b69a 31 ok2qCCWqWWiTjTzvHE93wTfKHDA resulting in the formation of fewer punctate eisosomes (Figure S1B). ------- COMMENT: c605477efc21b69a 32 zDsD+3G2Ft9T7qz0dog8QAK01OI decreased Pil1 protein abundance Figure S1F ------- COMMENT: c605477efc21b69a 33 I7smu3FBJ2vaOTrbVFyr4kUQ3QU (comment: cortical) ------- COMMENT: c605477efc21b69a 34 WdJ0ETVFC1RAP+7RnVv1e272Clg Pil1 lacking the C terminus failed to interact with Scs2 (Figure S5F ------- COMMENT: c605477efc21b69a 36 MHZTQrtI2W3A7aChbPgGbeOTSAs (Figure 4, S4B). ------- COMMENT: c60e704e5f4cb1e4 3 aUIUvJ5XT4DhSyPDWd//S1yR4is (comment: filter binding assay) ------- COMMENT: c60e704e5f4cb1e4 12 aUIUvJ5XT4DhSyPDWd//S1yR4is (comment: filter binding assay) ------- COMMENT: c60e704e5f4cb1e4 13 aUIUvJ5XT4DhSyPDWd//S1yR4is (comment: filter binding assay) ------- COMMENT: c60e704e5f4cb1e4 15 aUIUvJ5XT4DhSyPDWd//S1yR4is (comment: filter binding assay) ------- COMMENT: c641c32144a9fceb 28 DvQjy5oOMjEQo+A5SIbei1LiguE (comment: CHECK ABOLISHED SEPARATION) ------- COMMENT: c641c32144a9fceb 29 DvQjy5oOMjEQo+A5SIbei1LiguE (comment: CHECK ABOLISHED SEPARATION) ------- COMMENT: c641c32144a9fceb 30 nXakfmA2zxKXqe8quppOhYcpA+k (comment: with re-replication) ------- COMMENT: c6538160af902d62 2 PxGYNeSxrV5PD+XotSQQNBxfnSo fig 1a ------- COMMENT: c6538160af902d62 3 BLE68/Dn7PbMviEbxgjUe9ef/vE fig 1a (comment: CHECK maintenence of) ------- COMMENT: c6538160af902d62 11 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: c6538160af902d62 12 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: c6538160af902d62 13 GAqy8t8jp/JwHdUGBgIUhMOswPI fig 8a ------- COMMENT: c6538160af902d62 14 GAqy8t8jp/JwHdUGBgIUhMOswPI fig 8a ------- COMMENT: c6538160af902d62 15 GAqy8t8jp/JwHdUGBgIUhMOswPI fig 8a ------- COMMENT: c6538160af902d62 16 GAqy8t8jp/JwHdUGBgIUhMOswPI fig 8a ------- COMMENT: c6538160af902d62 17 FmN9hI9np/JNf5Ji4weHDLzwI7Q figure 9 ------- COMMENT: c6538160af902d62 19 TJJPmjKmxVLeaPQVDBwgc9sSnRc fig 8b ------- COMMENT: c6538160af902d62 20 TJJPmjKmxVLeaPQVDBwgc9sSnRc fig 8b ------- COMMENT: c6538160af902d62 23 J88iMmtD1vy5e0yMPMB5BOF2oog fig8d ------- COMMENT: c6538160af902d62 24 J88iMmtD1vy5e0yMPMB5BOF2oog fig8d ------- COMMENT: c6538160af902d62 25 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: c6538160af902d62 26 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: c6aa95e6f262f89b 19 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: c6aa95e6f262f89b 20 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: c6aa95e6f262f89b 22 /hViNJYvOTlbBkGwC4uLSOXAvyM Fig. 3B, C ------- COMMENT: c6aa95e6f262f89b 23 ycX2lBAAq244MxuLrK65bW/SdoY Fig. 3B, C We therefore concluded that Shu1 is required for hemin acquisition when hemin is present at very low concentrations (0.075 􏰄M), whereas its presence is dispens- able under conditions of high hemin concentrations ------- COMMENT: c6aa95e6f262f89b 24 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: c6aa95e6f262f89b 25 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: c6aa95e6f262f89b 39 P44aUkKFU6DlweZLBlyh9HvB+IY (comment: assayed using heme analog ZnMP) ------- COMMENT: c6aa95e6f262f89b 40 P44aUkKFU6DlweZLBlyh9HvB+IY (comment: assayed using heme analog ZnMP) ------- COMMENT: c6aa95e6f262f89b 41 P44aUkKFU6DlweZLBlyh9HvB+IY (comment: assayed using heme analog ZnMP) ------- COMMENT: c6ced0325cbf00af 8 W0YHb7oTUY3Jw/782f3eohfNbvY (comment: CHECK increased staining of all chromatin) ------- COMMENT: c6ced0325cbf00af 18 y5c9Eu9bDfxLN/gPCZ9F8z2ifBg (comment: assayed using minichromosomes and internal telomeric repeat arrays) ------- COMMENT: c6d09a7812ec9301 11 iKQ/iCzYV92/mpppGyhhu8m6v1A (Fig. 2b) D-apl2 and D-apm1 cells was completely inhibited in the presence of FK506 ------- COMMENT: c6d09a7812ec9301 12 iKQ/iCzYV92/mpppGyhhu8m6v1A (Fig. 2b) D-apl2 and D-apm1 cells was completely inhibited in the presence of FK506 ------- COMMENT: c6d09a7812ec9301 13 Putq8xuZR0KKpOYOJEErtB9FWec (Fig. 2b) whereas that of Dapl4 and Daps1 cells was partially inhibited ------- COMMENT: c6d09a7812ec9301 14 RKp3uXu1dubzqZkgSnEnUSSIDnc Dapl2 and Dapm1 cells were more sensitive when exposed to 34 °C or to 5 mM VPA com- pared with those of Dapl4 and Daps1 cells ------- COMMENT: c6d09a7812ec9301 15 RKp3uXu1dubzqZkgSnEnUSSIDnc Dapl2 and Dapm1 cells were more sensitive when exposed to 34 °C or to 5 mM VPA com- pared with those of Dapl4 and Daps1 cells ------- COMMENT: c6d09a7812ec9301 18 RKp3uXu1dubzqZkgSnEnUSSIDnc Dapl2 and Dapm1 cells were more sensitive when exposed to 34 °C or to 5 mM VPA com- pared with those of Dapl4 and Daps1 cells ------- COMMENT: c6d09a7812ec9301 19 RKp3uXu1dubzqZkgSnEnUSSIDnc Dapl2 and Dapm1 cells were more sensitive when exposed to 34 °C or to 5 mM VPA com- pared with those of Dapl4 and Daps1 cells ------- COMMENT: c6d09a7812ec9301 22 RKp3uXu1dubzqZkgSnEnUSSIDnc Dapl2 and Dapm1 cells were more sensitive when exposed to 34 °C or to 5 mM VPA com- pared with those of Dapl4 and Daps1 cells ------- COMMENT: c6d09a7812ec9301 29 BlmGgLi1RP+1/njKa8IWgzRMZhk Fig4. suggesting that the four adaptin subunits of the AP-1 complex are all localized to the Golgi ⁄ endosomes. ------- COMMENT: c6d09a7812ec9301 37 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: c6d09a7812ec9301 38 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: c6d09a7812ec9301 39 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: c6d09a7812ec9301 40 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: c6d09a7812ec9301 41 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: c6d09a7812ec9301 42 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: c6d09a7812ec9301 43 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: c6d09a7812ec9301 44 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: c6d09a7812ec9301 45 KPhWexYwWx8Og5c/5RIEg3LbEF4 Fig. 6a, wt, arrowheads ------- COMMENT: c6d09a7812ec9301 46 KPhWexYwWx8Og5c/5RIEg3LbEF4 Fig. 6a, wt, arrowheads ------- COMMENT: c6d09a7812ec9301 47 ySY+AXrbKViuUqmPFaBHC6s10jk (comment: localized to large endosomal structures) ------- COMMENT: c6d09a7812ec9301 48 ySY+AXrbKViuUqmPFaBHC6s10jk (comment: localized to large endosomal structures) ------- COMMENT: c6d09a7812ec9301 49 ySY+AXrbKViuUqmPFaBHC6s10jk (comment: localized to large endosomal structures) ------- COMMENT: c6d09a7812ec9301 50 ySY+AXrbKViuUqmPFaBHC6s10jk (comment: localized to large endosomal structures) ------- COMMENT: c6d09a7812ec9301 51 qHh2L/Ryi9Mm91bAT7yKiLbkC9c n wild-type cells, most of Krp1- red fluorescent protein (RFP) colocalized with GFP- Vrg4 (Fig. 7a), indicating that Krp1 mainly localized to the Golgi apparatus ------- COMMENT: c6d09a7812ec9301 52 qHh2L/Ryi9Mm91bAT7yKiLbkC9c n wild-type cells, most of Krp1- red fluorescent protein (RFP) colocalized with GFP- Vrg4 (Fig. 7a), indicating that Krp1 mainly localized to the Golgi apparatus ------- COMMENT: c6d09a7812ec9301 53 ySY+AXrbKViuUqmPFaBHC6s10jk (comment: localized to large endosomal structures) ------- COMMENT: c6d09a7812ec9301 54 ySY+AXrbKViuUqmPFaBHC6s10jk (comment: localized to large endosomal structures) ------- COMMENT: c6d09a7812ec9301 55 ySY+AXrbKViuUqmPFaBHC6s10jk (comment: localized to large endosomal structures) ------- COMMENT: c6d09a7812ec9301 56 ySY+AXrbKViuUqmPFaBHC6s10jk (comment: localized to large endosomal structures) ------- COMMENT: c6d1b50aea743116 19 yt5MZpCOyzj+niTOCjv6H/N0ot0 decrease in Gad8 phosphorylation ------- COMMENT: c6d1b50aea743116 30 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: c6d1b50aea743116 31 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: c6d1b50aea743116 32 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: c6d1b50aea743116 34 sDA6jf/k9+xPKgzlhAE8KgfnesA fig2b ------- COMMENT: c6d1b50aea743116 36 sDA6jf/k9+xPKgzlhAE8KgfnesA fig2b ------- COMMENT: c6d1b50aea743116 38 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: c6d1b50aea743116 39 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: c6d1b50aea743116 40 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: c6d1b50aea743116 41 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: c6d1b50aea743116 42 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: c6d1b50aea743116 43 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: c6d1b50aea743116 44 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: c6d1b50aea743116 45 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: c6d1b50aea743116 46 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: c6d1b50aea743116 47 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: c6d1b50aea743116 48 pi8tR/OzhaiNy9nAOGhKmN/XxhM Fig. 3c ------- COMMENT: c6d1b50aea743116 49 pi8tR/OzhaiNy9nAOGhKmN/XxhM Fig. 3c ------- COMMENT: c6d1b50aea743116 50 i9bSzgxod78OSfjL38pWix/bruA Fig. S3B ------- COMMENT: c6d1b50aea743116 51 i9bSzgxod78OSfjL38pWix/bruA Fig. S3B ------- COMMENT: c6d1b50aea743116 52 i9bSzgxod78OSfjL38pWix/bruA Fig. S3B ------- COMMENT: c6f8a329b174d4e5 1 qYfIlMLb9Qnp/C3Oq38sY5ApFzU respiration deficient: Glycerol is a carbon source that can be metabolized only through oxidative phosphorylation, producing ATP through the electron transport chain in the mitochondria. . Like other respiration- deficient mutants, the reduced growth rate of the ppr2 mutant on this medium indicates that it is not able to effectively carry out this process. ------- COMMENT: c6f8a329b174d4e5 2 qYfIlMLb9Qnp/C3Oq38sY5ApFzU respiration deficient: Glycerol is a carbon source that can be metabolized only through oxidative phosphorylation, producing ATP through the electron transport chain in the mitochondria. . Like other respiration- deficient mutants, the reduced growth rate of the ppr2 mutant on this medium indicates that it is not able to effectively carry out this process. ------- COMMENT: c6f8a329b174d4e5 3 Dzi9Dg9vpTnwU4ipnR7tm3kOBXA We found that Sdh2 and Hem15 were downregulated at the protein levels in Δppr2 cells (Fig. 1B) ------- COMMENT: c6f8a329b174d4e5 4 Dzi9Dg9vpTnwU4ipnR7tm3kOBXA We found that Sdh2 and Hem15 were downregulated at the protein levels in Δppr2 cells (Fig. 1B) ------- COMMENT: c6f8a329b174d4e5 5 Dzi9Dg9vpTnwU4ipnR7tm3kOBXA We found that Sdh2 and Hem15 were downregulated at the protein levels in Δppr2 cells (Fig. 1B) ------- COMMENT: c6f8a329b174d4e5 6 furlvW07g8PuLWIUPeUbn79CeGs The results in our qRT-PCR analysis on the genes of hem15 showed to be slightly down- regulated and the expression of sdh2 was not significantly altered by deletion of ppr2 (Fig. S1). ------- COMMENT: c6f8a329b174d4e5 7 7sfPs3ZyQqPHy1TtmumI+jgxAQo . Interestingly, deletion of ppr2 increased Gpx1 in cytosolic, whereas decreased Gpx1 in the mitochondria (Fig. 2A). ------- COMMENT: c6f8a329b174d4e5 8 7sfPs3ZyQqPHy1TtmumI+jgxAQo . Interestingly, deletion of ppr2 increased Gpx1 in cytosolic, whereas decreased Gpx1 in the mitochondria (Fig. 2A). ------- COMMENT: c6f8a329b174d4e5 10 TidH5Ly8bhCR4TJ3qcTjs85EwI4 In Δppr2 cells, Php4-GFP was localized in the nucleus at time points from 6 h to 36 h, in iron-replete conditions. In wild-type strains, Php4-GFP was exported from the nucleus, exhibiting fluorescence in the cytoplasm of cells from 6hto36h(Fig.3). ------- COMMENT: c6f8a329b174d4e5 11 6Q5FNOYjij2tjIKOjdIZCMmOLyM The levels of ROS in Ppr2-deficient cells were significantly higher compared to WT cells (Fig. 4A). The ppr2 deletion mutant was sensitive to H2O2 during the stationary phase, whereas WT retained tolerance to H2O2 (Fig. 4B). ------- COMMENT: c6f8a329b174d4e5 12 LytuUJlYopdsHeqbGGnfVaSGeGA (figure 4) (comment: vw: not mentioned in text) ------- COMMENT: c6f8a329b174d4e5 13 HyWRePm34zzMqyaI4BhesLzlGVg We found that Rsv2, Zym1 and Gst2 were upregulated both at the mRNA and protein levels in Δppr2 cells in exponential phase (6 h after incu- bation) (Fig. 5). The protein levels of Sod2 was increased in Δppr2 cells but their mRNA levels were unchanged (Fig. 5). ------- COMMENT: c6f8a329b174d4e5 14 HyWRePm34zzMqyaI4BhesLzlGVg We found that Rsv2, Zym1 and Gst2 were upregulated both at the mRNA and protein levels in Δppr2 cells in exponential phase (6 h after incu- bation) (Fig. 5). The protein levels of Sod2 was increased in Δppr2 cells but their mRNA levels were unchanged (Fig. 5). ------- COMMENT: c6f8a329b174d4e5 15 HyWRePm34zzMqyaI4BhesLzlGVg We found that Rsv2, Zym1 and Gst2 were upregulated both at the mRNA and protein levels in Δppr2 cells in exponential phase (6 h after incu- bation) (Fig. 5). The protein levels of Sod2 was increased in Δppr2 cells but their mRNA levels were unchanged (Fig. 5). ------- COMMENT: c6f8a329b174d4e5 16 HyWRePm34zzMqyaI4BhesLzlGVg We found that Rsv2, Zym1 and Gst2 were upregulated both at the mRNA and protein levels in Δppr2 cells in exponential phase (6 h after incu- bation) (Fig. 5). The protein levels of Sod2 was increased in Δppr2 cells but their mRNA levels were unchanged (Fig. 5). ------- COMMENT: c6f8a329b174d4e5 17 HyWRePm34zzMqyaI4BhesLzlGVg We found that Rsv2, Zym1 and Gst2 were upregulated both at the mRNA and protein levels in Δppr2 cells in exponential phase (6 h after incu- bation) (Fig. 5). The protein levels of Sod2 was increased in Δppr2 cells but their mRNA levels were unchanged (Fig. 5). ------- COMMENT: c6f8a329b174d4e5 18 QehamcFLnPK9FE5OI0JNKJJbbJs 3.7. Sty1 and Pap1 accumulate in the nucleus during the stationary phase in Δppr2 cells ------- COMMENT: c6f8a329b174d4e5 19 QehamcFLnPK9FE5OI0JNKJJbbJs 3.7. Sty1 and Pap1 accumulate in the nucleus during the stationary phase in Δppr2 cells ------- COMMENT: c6f8a329b174d4e5 20 sUpsDVlOBzD1G8C4raCYHJvTyxg We observed reduced growth of the ppr2 deletion mutant on glycerol- and galactose-containing media compared to on the glucose-containing media (Fig. 1A). ------- COMMENT: c6f8a329b174d4e5 21 sUpsDVlOBzD1G8C4raCYHJvTyxg We observed reduced growth of the ppr2 deletion mutant on glycerol- and galactose-containing media compared to on the glucose-containing media (Fig. 1A). ------- COMMENT: c6f8a329b174d4e5 22 sUpsDVlOBzD1G8C4raCYHJvTyxg We observed reduced growth of the ppr2 deletion mutant on glycerol- and galactose-containing media compared to on the glucose-containing media (Fig. 1A). ------- COMMENT: c6f8a329b174d4e5 23 3eB2SPs6OS5sMAbl05dsZ9olJzU Figure 2C control We found that the overexpression of Gpx1 could rescue the viability of Δppr2 cells as Gpx1-HA showed significantly increased viability compared with Δppr2 cells and similar to WT strain on YE plates (Fig. 2C). Additionally, overexpression of Gpx1 could also partially rescue respiratory growth defect in Δppr2 cells as Gpx1-HA could grow on Gly plates better than Δppr2 cells (Fig. 2D). ------- COMMENT: c6f8a329b174d4e5 24 HyWRePm34zzMqyaI4BhesLzlGVg We found that Rsv2, Zym1 and Gst2 were upregulated both at the mRNA and protein levels in Δppr2 cells in exponential phase (6 h after incu- bation) (Fig. 5). The protein levels of Sod2 was increased in Δppr2 cells but their mRNA levels were unchanged (Fig. 5). ------- COMMENT: c6f8a329b174d4e5 25 HyWRePm34zzMqyaI4BhesLzlGVg We found that Rsv2, Zym1 and Gst2 were upregulated both at the mRNA and protein levels in Δppr2 cells in exponential phase (6 h after incu- bation) (Fig. 5). The protein levels of Sod2 was increased in Δppr2 cells but their mRNA levels were unchanged (Fig. 5). ------- COMMENT: c6f8a329b174d4e5 26 HyWRePm34zzMqyaI4BhesLzlGVg We found that Rsv2, Zym1 and Gst2 were upregulated both at the mRNA and protein levels in Δppr2 cells in exponential phase (6 h after incu- bation) (Fig. 5). The protein levels of Sod2 was increased in Δppr2 cells but their mRNA levels were unchanged (Fig. 5). ------- COMMENT: c6fe4aad56b8dbb9 57 Z3H2JED9pF0obpyDehQ/rdO5iJI (comment: bulk antisense transcripts) ------- COMMENT: c6fe4aad56b8dbb9 60 /UOK7yEkLUNQ8SiA34JElBzKwKI (comment: sense strand) ------- COMMENT: c6fe4aad56b8dbb9 62 Z3H2JED9pF0obpyDehQ/rdO5iJI (comment: bulk antisense transcripts) ------- COMMENT: c6fe4aad56b8dbb9 63 Z3H2JED9pF0obpyDehQ/rdO5iJI (comment: bulk antisense transcripts) ------- COMMENT: c6fe4aad56b8dbb9 64 Z3H2JED9pF0obpyDehQ/rdO5iJI (comment: bulk antisense transcripts) ------- COMMENT: c6fe4aad56b8dbb9 65 Z3H2JED9pF0obpyDehQ/rdO5iJI (comment: bulk antisense transcripts) ------- COMMENT: c6fe4aad56b8dbb9 66 Z3H2JED9pF0obpyDehQ/rdO5iJI (comment: bulk antisense transcripts) ------- COMMENT: c6fe4aad56b8dbb9 67 Z3H2JED9pF0obpyDehQ/rdO5iJI (comment: bulk antisense transcripts) ------- COMMENT: c6fe4aad56b8dbb9 81 Z3H2JED9pF0obpyDehQ/rdO5iJI (comment: bulk antisense transcripts) ------- COMMENT: c6fe4aad56b8dbb9 82 Z3H2JED9pF0obpyDehQ/rdO5iJI (comment: bulk antisense transcripts) ------- COMMENT: c6fe4aad56b8dbb9 83 Z3H2JED9pF0obpyDehQ/rdO5iJI (comment: bulk antisense transcripts) ------- COMMENT: c6fe4aad56b8dbb9 95 uGXCB/Uj8HXgCuBcE0c7AyMZv7g (comment: transcript levels - antisense & cen forward strand) ------- COMMENT: c6fe4aad56b8dbb9 96 uGXCB/Uj8HXgCuBcE0c7AyMZv7g (comment: transcript levels - antisense & cen forward strand) ------- COMMENT: c72c3efc43c47d0d 3 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: c72c3efc43c47d0d 4 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: c72c3efc43c47d0d 5 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: c72c3efc43c47d0d 6 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: c72c3efc43c47d0d 8 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: c72c3efc43c47d0d 9 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: c75277b83a634a8d 2 CN8BURm4PNOYqsiaPLAD1uBuzWQ Fig 1b (comment: CHECK me2) ------- COMMENT: c75277b83a634a8d 3 4ZwYdvVssBbgy2xmChFeF1Mi/qc Fig 1b (comment: CHECK (1.5x) (me2)) ------- COMMENT: c75277b83a634a8d 4 2VhR2CGHwbHILI6ZzBBqRnOjTSo Extended data Fig 1b, c (comment: also at MTREC independent islands) ------- COMMENT: c75277b83a634a8d 5 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: c75277b83a634a8d 6 islrucHx9huQK3ul0duymUg80+E figure 1e ------- COMMENT: c75277b83a634a8d 7 eCMi+2+M79gobfQ4X9J4EIrKsGA Fig 1a ------- COMMENT: c75277b83a634a8d 8 DMulf00G+N8hu7sffxlLcYryiVY Fig. 1f, g ------- COMMENT: c75277b83a634a8d 9 GEppxU37rZjhEcgwcDD+JNbTBF4 figure 1h ------- COMMENT: c75277b83a634a8d 10 F/7jROw6xdIMZzsgbR5pxY/Pddw Moreover, ubiquitination of Pir1 was detected in tor2-ts6 cells and increased in the tor2-ts6 mts2-1 mutant (Fig. 1h) ------- COMMENT: c75277b83a634a8d 11 eCMi+2+M79gobfQ4X9J4EIrKsGA Fig 1a ------- COMMENT: c75277b83a634a8d 16 NgHhi1OjrXAPI5RHMMk1rdI4pwc he Tor2-containing TORC1 complex phosphorylated Pir1 in vitro and mutation of the 12 serine residues to alanine attenu- ated Pir1 phosphorylation (Extended Data Fig. 2a,c). ------- COMMENT: c75277b83a634a8d 17 UpTBEbvpM4zjoYKBQOyCageiNgY Extended Data Fig. 2a, c ------- COMMENT: c75277b83a634a8d 18 6p6GBip/XuObcEpcuvke3LLmLMU Replacement of the 12 phospho-serine residues with the phospho-mimic aspartic acid residue (Pir1-12SD) indeed conferred stability in tor2-ts6 cells (Extended Data Fig. 2d) ------- COMMENT: c75277b83a634a8d 19 TRcChrtohsZ3vEOlsgkK5zht1VU Replacement of the 12 phospho-serine residues with the phospho-mimic aspartic acid residue (Pir1-12SD) indeed conferred stability in tor2-ts6 cells (Extended Data Fig. 2d); Deletion of ubi4 in tor2-ts6 cells cells indeed stabilized Pir1 (Fig. 3b) ------- COMMENT: c75277b83a634a8d 20 pNubJYTDdbDCxxx6gi0YUXltWqg Consistently, ubiquitination of Pir1-SD in the tor2-ts6 mts2-1 mutant was reduced (Fig. 2e). ------- COMMENT: c75277b83a634a8d 21 RnTxnVhZIyQU4+9ETItvAblX/Qw Interestingly, the expression of Pir1-SD in tor2-ts6 cells restored the levels of Red1 (Extended Data Fig. 2e), suggesting that the reduction in Red1 in the tor2 mutant cells (Fig. 1c) is linked to the degradation of its interaction part- ner Pir1 ------- COMMENT: c75277b83a634a8d 22 wPoylQfe01oVAKtebI/yTe6c4aU ubi4 gene, which encodes polyubiquitin implicated in sexual development34, was upregulate in tor2-ts6 cells (Fig. 3a). ------- COMMENT: c75277b83a634a8d 25 tgqtF5fePX1XNHFK7aqdqO4jjE8 Moreover, loss of the ubiquitin ligase-associated Cullin-RING finger family protein Cul4, a component of ClrC35,36 that interacts with MTREC15, also stabilized Pir1 in both tor2-ts6 and nitrogen-starved cells (Fig. 3c ------- COMMENT: c75277b83a634a8d 28 tgqtF5fePX1XNHFK7aqdqO4jjE8 Moreover, loss of the ubiquitin ligase-associated Cullin-RING finger family protein Cul4, a component of ClrC35,36 that interacts with MTREC15, also stabilized Pir1 in both tor2-ts6 and nitrogen-starved cells (Fig. 3c ------- COMMENT: c75277b83a634a8d 29 J+aZo87z2sbDjTKYePXhN2RxcP0 The addition of ubi4∆, cul4∆ or ddb1∆ dramatically reduced Pir1 ubiquitination in tor2-ts6 mts2-1 cells (Fig. 3f). ------- COMMENT: c75277b83a634a8d 30 J+aZo87z2sbDjTKYePXhN2RxcP0 The addition of ubi4∆, cul4∆ or ddb1∆ dramatically reduced Pir1 ubiquitination in tor2-ts6 mts2-1 cells (Fig. 3f). ------- COMMENT: c75277b83a634a8d 31 J+aZo87z2sbDjTKYePXhN2RxcP0 The addition of ubi4∆, cul4∆ or ddb1∆ dramatically reduced Pir1 ubiquitination in tor2-ts6 mts2-1 cells (Fig. 3f). ------- COMMENT: c75277b83a634a8d 36 Kg6vzJ844YZoVhDtwXmGOdJw7EI (comment: which?) ------- COMMENT: c75277b83a634a8d 37 Kg6vzJ844YZoVhDtwXmGOdJw7EI (comment: which?) ------- COMMENT: c75277b83a634a8d 38 h/BiE+4dTGxu4hy7OZOzG32e1Zg the deletion of ubi4, ddb1 or cul4 restored ade6-DSR silencing (Fig. 3g and Extended Data Fig. 3d). ------- COMMENT: c75277b83a634a8d 39 h/BiE+4dTGxu4hy7OZOzG32e1Zg the deletion of ubi4, ddb1 or cul4 restored ade6-DSR silencing (Fig. 3g and Extended Data Fig. 3d). ------- COMMENT: c75277b83a634a8d 40 h/BiE+4dTGxu4hy7OZOzG32e1Zg the deletion of ubi4, ddb1 or cul4 restored ade6-DSR silencing (Fig. 3g and Extended Data Fig. 3d). ------- COMMENT: c75277b83a634a8d 41 ePofpz/r3+g/dcJS8VbxF1ykSa4 The restoration of silencing required Pir1, as a loss of Ubi4 failed to silence ade6-DSR in pir1∆ cells (Fig. 3g). ------- COMMENT: c75277b83a634a8d 42 ePofpz/r3+g/dcJS8VbxF1ykSa4 The restoration of silencing required Pir1, as a loss of Ubi4 failed to silence ade6-DSR in pir1∆ cells (Fig. 3g). ------- COMMENT: c75277b83a634a8d 43 i9NZTP7+YcjI/9LrwNAfnb0uE98 Remarkably, the loss of Ubi4, Cul4 or Ddb1 in tor2-ts6 cells restored the silencing of gametogenic genes genome-wide (Fig. 3h and Extended Data Fig. 3g). ------- COMMENT: c75277b83a634a8d 44 E5unkbncUBjBVV5xdglXT4J9Q3U Remarkably, the loss of Ubi4, Cul4 or Ddb1 in tor2-ts6 cells restored the silencing of gametogenic genes genome-wide (Fig. 3h and Extended Data Fig. 3g). and Fig. 4g). ------- COMMENT: c75277b83a634a8d 45 i9NZTP7+YcjI/9LrwNAfnb0uE98 Remarkably, the loss of Ubi4, Cul4 or Ddb1 in tor2-ts6 cells restored the silencing of gametogenic genes genome-wide (Fig. 3h and Extended Data Fig. 3g). ------- COMMENT: c75277b83a634a8d 46 POGU6mtcQy0k8BiGhIlpbVRCXyM Intriguingly, cells expressing Pir1-SD, but not Pir1-WT or Pir1-SA, continued to divide on nutrient-limiting medium at a low temperature (Fig. 4a), suggesting that stabilized Pir1 supports cell proliferation under suboptimal growth conditions. ------- COMMENT: c75277b83a634a8d 47 dYxxVnj0Yj1xPXOm8RCwBfWOl3k We indeed observed that pir1∆ cells exhibited a growth defect on minimal medium ------- COMMENT: c75277b83a634a8d 48 dYxxVnj0Yj1xPXOm8RCwBfWOl3k We indeed observed that pir1∆ cells exhibited a growth defect on minimal medium ------- COMMENT: c75277b83a634a8d 49 hZ5fBD2KTaNYItu98fN9/yvDsUE Similar to pir1∆, tor2-ts6 cells showed a severe growth defect at a semi-permissive temperature (29°C) on minimal medium but not on rich medium (Fig. 4d). ------- COMMENT: c75277b83a634a8d 50 hZ5fBD2KTaNYItu98fN9/yvDsUE Similar to pir1∆, tor2-ts6 cells showed a severe growth defect at a semi-permissive temperature (29°C) on minimal medium but not on rich medium (Fig. 4d). ------- COMMENT: c75277b83a634a8d 51 0XFcZH9Dc+Gxb0ZhkkXkOn45HQA Notably, Pir1 was depleted during early meiosis (Fig. 5a) but gradually recov- ered by middle meiosis. ------- COMMENT: c75277b83a634a8d 52 xK62SVZOfaB4Bn7HzZADrWmsryY Whereas Pir1-WT disappeared, Pir1-SD per- sisted during meiosis as multiple nuclear foci coinciding with Mmi1 and Erh1 foci (Fig. 5b,c). ------- COMMENT: c75277b83a634a8d 53 k2SZW7s8gz9PJyXCrfrmyjZuieo restores the MTREC and Rrp6 association with Mmi1 and Erh1 during meiosis (Fig. 5d). ------- COMMENT: c75277b83a634a8d 54 k2SZW7s8gz9PJyXCrfrmyjZuieo restores the MTREC and Rrp6 association with Mmi1 and Erh1 during meiosis (Fig. 5d). ------- COMMENT: c75277b83a634a8d 55 WkjhE3NHUkV78Hkd0Gwp4vXWG78 Figure 6B DSBs; for example, rec25, rec27 and mug20), which are critical for recombination and proper chromosome segregation during meiosis-I4 ------- COMMENT: c75277b83a634a8d 56 Vk/aVSTJ3JGuwFkyTqVF2/UbX8Y Compared with the WT, cells expressing Pir1-SD showed a marked decrease in recombination frequency (Fig. 6f). ------- COMMENT: c75277b83a634a8d 57 gVktz7FIsA4+gLrzGmTSS2X8lGU cells showed impaired oscillation of chromosomes and a prolonged horsetail stage (approximately 160min compared with approximately 120min; Fig. 7a,b). ------- COMMENT: c75277b83a634a8d 58 JuTWCOIZyS6s/iXm1rCFuA6nBxc Defective chromosome segregation and reduced spore viability were also noted (Fig. 7a and Supplementary Videos 1–3) ------- COMMENT: c75277b83a634a8d 59 JuTWCOIZyS6s/iXm1rCFuA6nBxc Defective chromosome segregation and reduced spore viability were also noted (Fig. 7a and Supplementary Videos 1–3) ------- COMMENT: c75277b83a634a8d 60 HiQ/EGgU+Yz94K3Z6SzMP+/Z3vU abnormal asci containing fewer than four, or no, spores were frequently generated (Fig. 7c). ------- COMMENT: c757e60bdb79c5cb 14 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 15 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 16 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 18 6CISp7Ikq4bJZIgWD74kQMdHhBs (comment: affects unspliced pre-mRNA) ------- COMMENT: c757e60bdb79c5cb 19 t/tea5tVO9FmMCwQXApMDaqdFOI (comment: CHECK gene locus: rps2202) ------- COMMENT: c757e60bdb79c5cb 20 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 21 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 22 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 23 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 24 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 25 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 26 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 27 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 28 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 29 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 30 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 31 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 32 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 33 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 34 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 35 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 36 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 37 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 38 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 41 Ag46ET3Up7GmyBy+zjivTfpN1s4 (comment: CHECK gene locus affected: rps2202) ------- COMMENT: c757e60bdb79c5cb 42 8/4xxekmxhw6XyBUCMkjUTrstP0 (comment: CHECK increase > 50-fold) ------- COMMENT: c757e60bdb79c5cb 43 U39do4jCjlHsxiQkHKmWATQyTqE (comment: CHECK increase > 10-fold) ------- COMMENT: c757e60bdb79c5cb 44 U39do4jCjlHsxiQkHKmWATQyTqE (comment: CHECK increase > 10-fold) ------- COMMENT: c757e60bdb79c5cb 45 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 46 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 47 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 48 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 50 U39do4jCjlHsxiQkHKmWATQyTqE (comment: CHECK increase > 10-fold) ------- COMMENT: c757e60bdb79c5cb 51 U39do4jCjlHsxiQkHKmWATQyTqE (comment: CHECK increase > 10-fold) ------- COMMENT: c757e60bdb79c5cb 52 U39do4jCjlHsxiQkHKmWATQyTqE (comment: CHECK increase > 10-fold) ------- COMMENT: c757e60bdb79c5cb 53 U39do4jCjlHsxiQkHKmWATQyTqE (comment: CHECK increase > 10-fold) ------- COMMENT: c757e60bdb79c5cb 54 U39do4jCjlHsxiQkHKmWATQyTqE (comment: CHECK increase > 10-fold) ------- COMMENT: c757e60bdb79c5cb 55 U39do4jCjlHsxiQkHKmWATQyTqE (comment: CHECK increase > 10-fold) ------- COMMENT: c757e60bdb79c5cb 56 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 57 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 58 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 59 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 60 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 61 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 62 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 63 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 64 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 65 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 66 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 67 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 68 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 69 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 70 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 71 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 72 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 73 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 74 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 75 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 76 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 77 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 78 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 79 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 81 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 82 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 83 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 84 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 85 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 86 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 87 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 88 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 89 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 90 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 91 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 92 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 93 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 94 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 95 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 96 dUgIhJlMUS+Hxh1AGjAtgbLYkJI (comment: CHECK SPCC1235.04c) ------- COMMENT: c757e60bdb79c5cb 97 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 98 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 99 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 100 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 101 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 102 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 103 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 104 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 105 j0Waee7iuNiANJUtnHAAHpgRNFM (comment: enrichment in CRAC > 10-fold; Mmi1 binds the 5' extended region of the overlapping regulatory lncRNA prt) ------- COMMENT: c757e60bdb79c5cb 106 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 107 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 108 SmbGR3g98X5sSZ/2fwcVUa9Lmsk (comment: CHECK SPBC1289.13c) ------- COMMENT: c757e60bdb79c5cb 109 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 110 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 111 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 112 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 113 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 114 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 115 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 116 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 117 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 118 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 119 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 120 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 121 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 122 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 123 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 124 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 125 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 126 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 127 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 128 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 129 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 130 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 131 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 132 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 133 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 134 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 135 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 136 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 137 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 138 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 139 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 140 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 141 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 142 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 143 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 144 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 145 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 146 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 147 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 148 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 149 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 150 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 151 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 152 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 153 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 154 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 155 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 156 eT214DIQ72sCUoDljBcC7tAt3LM (comment: enrichment in CRAC > 10-fold) ------- COMMENT: c757e60bdb79c5cb 157 5R9I6B/byYR/QuWdqkuxMLTZo3k (comment: CHECK increase > 40-fold) ------- COMMENT: c757e60bdb79c5cb 158 U39do4jCjlHsxiQkHKmWATQyTqE (comment: CHECK increase > 10-fold) ------- COMMENT: c757e60bdb79c5cb 159 U39do4jCjlHsxiQkHKmWATQyTqE (comment: CHECK increase > 10-fold) ------- COMMENT: c757e60bdb79c5cb 160 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 162 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 163 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 164 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 165 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 166 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 167 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 168 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 170 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 171 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 172 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 173 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 174 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 175 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 176 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 178 Osiw42ag5uHtiiDJIe9ImorogrE (comment: CHECK gene locus affected: dbp2) ------- COMMENT: c757e60bdb79c5cb 179 pUTRyR5NhzhoYgQn4x2F5mt/jBk (comment: CHECK gene affected: rps2202) ------- COMMENT: c757e60bdb79c5cb 181 v9lTfVoSULS5Z9/VSmI9gxKk5kA Fig. S4B ------- COMMENT: c757e60bdb79c5cb 187 sMknIdBM1KGBwSJEDQEe/fy6yLM fig S5e ------- COMMENT: c757e60bdb79c5cb 188 5jJms7yFbVgfPtX4c7gIeevfWFI (comment: CHECK increase > 5-fold) ------- COMMENT: c757e60bdb79c5cb 189 x3vqtwhjzCjYkzE06e1k9lfzvx8 (comment: enrichment in CRAC > 10-fold (vw changed dpp2->dbp2)) ------- COMMENT: c796a227230bddce 32 pV3ioMmgkB9sur5lv9Jv7LonqE0 (comment: CHECK curved microtubule phenotype) ------- COMMENT: c7de46ee1570b585 3 NT1++07EPIXtOGaknKaG5+E+b/A Fig1 In early G2 nif1 localisation is monopolar and in late G2 it is bipolar ------- COMMENT: c7de46ee1570b585 5 Ci+bKhUitsutwHeHgf5TiETS96Y Fig1 ------- COMMENT: c7de46ee1570b585 6 Ci+bKhUitsutwHeHgf5TiETS96Y Fig1 ------- COMMENT: c7de46ee1570b585 7 Ci+bKhUitsutwHeHgf5TiETS96Y Fig1 ------- COMMENT: c7de46ee1570b585 8 Ci+bKhUitsutwHeHgf5TiETS96Y Fig1 ------- COMMENT: c7de46ee1570b585 13 vba9inRnwgihM2NToBTFG/tYq9k Fig3, Table 1 ------- COMMENT: c7de46ee1570b585 19 vba9inRnwgihM2NToBTFG/tYq9k Fig3, Table 1 ------- COMMENT: c7de46ee1570b585 20 vba9inRnwgihM2NToBTFG/tYq9k Fig3, Table 1 ------- COMMENT: c7de46ee1570b585 21 nFLM/g3imMnyxit4YBXi1PBGe94 Fig 6, shows pom1delta cells still have G2-M size control ------- COMMENT: c7de46ee1570b585 22 8TDEbaHbxXD5rnjWZPBD61qteh0 Fig 6, shows nif1delta cells still have G2-M size control ------- COMMENT: c7de46ee1570b585 23 Om0wKM+ZHkxfMdqAc3b0JzvDnuY Fig 6, shows wee1-50 cells at restrictive temperature have lost G2-M size control ------- COMMENT: c7dfdbd8467f6d60 1 P6ccOFL3CInIdI2xBAOPCe3Z/Mo Fig1. (comment: CHECK 31.94% longer than control mean) ------- COMMENT: c7dfdbd8467f6d60 2 A4Y+jCq0d8TydAeTVB7D2CPcgKs Fig3. (comment: CHECK Dea2 level reduced to 50%) ------- COMMENT: c7dfdbd8467f6d60 3 rpmrVhJmFMfhPGCdUylu31ZA8nU Fig1. (comment: CHECK 23.46% longer than control mean) ------- COMMENT: c7dfdbd8467f6d60 4 c8RgzUvrdUAxdvpaxpbrzhAPu24 Fig3. (comment: CHECK Nup184 level reduced to 30% decreased protein level in heterozygous diploid cell during vegetative growth) ------- COMMENT: c7dfdbd8467f6d60 5 +Vs5iBCXof5f/BGQ5HeEiRyeqV8 Fig1. (comment: CHECK 19.29% longer than control mean) ------- COMMENT: c7dfdbd8467f6d60 6 i+BcJDrG2fRMS9T/gE+TIE3BW8Y Fig3. (comment: CHECK Nsp1 level reduced to ~45% decreased protein level in heterozygous diploid cell during vegetative growth) ------- COMMENT: c7dfdbd8467f6d60 7 aMG+n28WM9wRV/4lFrVTTzAs570 Fig1. (comment: CHECK 18.92% longer than control mean) ------- COMMENT: c7dfdbd8467f6d60 8 UrCXg0HJFboOCQF1gWDGOvuas1A Fig3 (comment: CHECK Nup97 level reduced to ~55% decreased protein level in heterozygous diploid cell during vegetative growth) ------- COMMENT: c7dfdbd8467f6d60 9 jtFAT7CaFDShsJSi1f8DdCiYfew Fig1. (comment: CHECK 16.78% longer than control mean) ------- COMMENT: c7dfdbd8467f6d60 10 a+mZGfLdeCV4TS+TeO3pUlZPgbM Fig3. (comment: CHECK cdc13 level reduced to ~44% decreased protein level in heterozygous diploid cell during vegetative growth) ------- COMMENT: c7dfdbd8467f6d60 11 3vlytcCMSWdzOpf6DK+kA0f8qlE Fig1. (comment: CHECK 15.82% longer than control mean) ------- COMMENT: c7dfdbd8467f6d60 12 FoW6J3RtKC+Hlr+8MvKg78E7+Dc Fig3. (comment: CHECK Nup186 level reduced to ~45% decreased protein level in heterozygous diploid cell during vegetative growth) ------- COMMENT: c7dfdbd8467f6d60 13 L+dj1IIdkq7CkJFmJpCgBjRbVJo Fig1. (comment: CHECK 15.55% longer than control mean) ------- COMMENT: c7dfdbd8467f6d60 14 lAztaPIqNOpd12Ucxp7cZcKjPd0 Fig3. (comment: CHECK cdc25 level reduced to ~48% decreased protein level in heterozygous diploid cell during vegetative growth) ------- COMMENT: c7dfdbd8467f6d60 15 zH4Tfyj4w1/HOhThDUT3CRaD03I Fig1. (comment: CHECK 14.43% longer than control mean) ------- COMMENT: c7dfdbd8467f6d60 16 EJC1dZ7GyN1P3VboArfVXkddh2E Fig3. (comment: CHECK Cdc2 level reduced to about 48% decreased protein level in heterozygous diploid cell during vegetative growth) ------- COMMENT: c7dfdbd8467f6d60 17 I/buNAg+5/s8YH+fYiCiE1iLu5Y Fig1. (comment: CHECK 11.63% longer than control mean) ------- COMMENT: c7dfdbd8467f6d60 18 i9UhwGB94zwRD97DKH2jt7+q51Q Fig3. (comment: CHECK Cdr1 level reduced to ~55% decreased protein level in heterozygous diploid cell during vegetative growth) ------- COMMENT: c7dfdbd8467f6d60 19 PFlt+AeYePbV6Li3ASfRE8uDCkE Fig1. (comment: CHECK 10.81% longer than control mean) ------- COMMENT: c7dfdbd8467f6d60 20 C8dQcb0bLp8Qcqbf06SeCDdNj6M Fig3. (comment: CHECK Sal3 level reduced to ~48% decreased protein level in heterozygous diploid cell during vegetative growth) ------- COMMENT: c7dfdbd8467f6d60 21 I2YLGxFhYTNJMVNl09ULibuySy8 Fig1. (comment: CHECK 8.80% longer than control mean) ------- COMMENT: c7dfdbd8467f6d60 22 Ukno0a6q/EhwFAuOZn7QTZ4drFY Fig3. (comment: CHECK nup45 level reduced to ~45% decreased protein level in heterozygous diploid cell during vegetative growth) ------- COMMENT: c7dfdbd8467f6d60 23 nX61gsgQlfVT+FNCWXXW+BbOEzQ Fig1. (comment: CHECK 8.78% longer than control mean) ------- COMMENT: c7dfdbd8467f6d60 24 7fXIzlCLjffAc0gsn429INnuaCE Fig3. (comment: CHECK Cpc2 level reduced to about 55% decreased protein level in heterozygous diploid cell during vegetative growth) ------- COMMENT: c7dfdbd8467f6d60 25 qhTX/GWLNgcmOwL5t4pjihrsQq8 Fig1. (comment: CHECK 8.74% longer than control mean) ------- COMMENT: c7dfdbd8467f6d60 27 3/5QEpIdvcRdJjkW0SCBvUU/cjE Fig1. (comment: CHECK 9.10% shorter than control mean) ------- COMMENT: c7dfdbd8467f6d60 28 L2pkgD8Y3viZFCdJxKJPgyULhRw Fig3. (comment: CHECK Ppa2 reduced to~45% decreased protein level in heterozygous diploid cell during vegetative growth) ------- COMMENT: c7dfdbd8467f6d60 29 SFMSZEHpkVsBlR3maGTVVdIOCXM Fig3. (comment: CHECK Suc1 level reduced to ~60% decreased protein level in heterozygous diploid cell during vegetative growth) ------- COMMENT: c7dfdbd8467f6d60 30 alur3/cIFYPakqnmsZElUNEm80A Fig1. (comment: CHECK 10.86% shorter than control mean) ------- COMMENT: c7dfdbd8467f6d60 31 Zx428Rm6SgqDaluBPoUvV0pU1Z0 Fig3. (comment: CHECK Pom1 level reduced to ~55% decreased protein level in heterozygous diploid cell during vegetative growth) ------- COMMENT: c7dfdbd8467f6d60 32 ZI+r4oYyTUf5OviYSxSLCD5XABI Fig1. (comment: CHECK 11.15% shorter than control mean) ------- COMMENT: c7dfdbd8467f6d60 33 rduLaoQ4uw0ynWRveEJWYmMEbZU Fig3 (comment: CHECK Wee1 level reduced to ~32% decreased protein level in heterozygous diploid cell during vegetative growth) ------- COMMENT: c7dfdbd8467f6d60 34 L7PldlAguK4lFMSXAk6F7+53tVM Fig1. (comment: CHECK 16.50% shorter than control mean) ------- COMMENT: c7dfdbd8467f6d60 35 pwYXrSYzRwbCu7+s5GUV52ElDQc Fig3. (comment: CHECK Nup189 level reduced to ~50% decreased protein level in heterozygous diploid cell during vegetative growth) ------- COMMENT: c7ee4622fd63b2da 36 uiBw2bUkFvvJEoyHoIMDrqByXTA cut if exposed to radiation during S phase, but not if exposed during G2 ------- COMMENT: c7ee4622fd63b2da 42 +eE8Du6JJo3sSovVA8CbnARfZqY constant level throughout cell cycle ------- COMMENT: c833cf237c7875e1 1 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: c833cf237c7875e1 2 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: c833cf237c7875e1 3 5gBn77UghFlKE5e+tRzMv++wslw Fig. S1 ------- COMMENT: c833cf237c7875e1 4 X8yCxtNQHQPNBovwToX1U5cVS7Q Fig. S1 (comment: Not really abnormal, should be just bipolar) ------- COMMENT: c833cf237c7875e1 5 WerjrIUVsTSt4TqMy4j23Tgkse4 Fig. S1 (comment: CHECK rescue of FYPO:0000276) ------- COMMENT: c833cf237c7875e1 6 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: c833cf237c7875e1 7 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: c833cf237c7875e1 8 +ryRJMUoBzRk9GxtxL1OQqWo1lI Fig. 2 (comment: main text) ------- COMMENT: c833cf237c7875e1 9 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: c833cf237c7875e1 10 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: c833cf237c7875e1 11 ZuupOLtyjchGhWMgI9w5z8wdSAw (comment: Main text (Figure S2 seems wrongly labelled)) ------- COMMENT: c833cf237c7875e1 12 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: c833cf237c7875e1 13 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: c833cf237c7875e1 14 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: c833cf237c7875e1 15 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: c833cf237c7875e1 16 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: c833cf237c7875e1 17 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: c833cf237c7875e1 18 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: c833cf237c7875e1 19 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: c833cf237c7875e1 20 7znRqTImsvzQstZpkTc+M4n46dw Fig. S4 ------- COMMENT: c833cf237c7875e1 21 6AgZlT2cFUVt2rqI9hNPq11VMxU Fig. S4 (comment: CHECK rescues growth HIGH TEMP) ------- COMMENT: c833cf237c7875e1 22 6AgZlT2cFUVt2rqI9hNPq11VMxU Fig. S4 (comment: CHECK rescues growth HIGH TEMP) ------- COMMENT: c833cf237c7875e1 23 /uCtgk6K/b0Cg+MgyWF3eTPoxTA Fig. S3E ------- COMMENT: c833cf237c7875e1 24 sPdK5LWSg/eWzP8fz6zLtxwarFg Fig. S3D and the fact that is required for bipolar spindle formation ------- COMMENT: c833cf237c7875e1 25 AGLRvfFeRQDMtABOEcb/CT+qlbw (comment: CHECK cut7D pkl1D ase1D lethal) ------- COMMENT: c833cf237c7875e1 26 tq3BSz6KZjr5OABmGfYoHwKfG04 (comment: CHECK cut7D pkl1D cls1off lethal) ------- COMMENT: c833cf237c7875e1 27 CnELwxTiXPjnbJKrbKJPtaa9jhU (comment: CHECK cut7D pkl1D klp9off does not elongate during anaphase B, cut7D pkl1D klp9D lethal, deleting all other kinesins except klp9 did not affect elongation after removing cut7) ------- COMMENT: c83c3de5e5b69049 2 rTdTRYDjA+sIIR2zj+Z8zlfO2hQ (comment: prevents bipolar attachment (Ask Takeshi if this fits better rec role)) ------- COMMENT: c83c3de5e5b69049 3 sonEvlApcHeTVOvj7sxmb3E2a1g (comment: prevents bipolar attachment) ------- COMMENT: c83c3de5e5b69049 4 sonEvlApcHeTVOvj7sxmb3E2a1g (comment: prevents bipolar attachment) ------- COMMENT: c83c3de5e5b69049 8 RfkN9nj2CD7lbgGLcbbbhREWFQU ( Figure 1A) continuous rate of spindle elongation (I) ------- COMMENT: c83c3de5e5b69049 9 pqSDB2DIGeEgjzV69OPmn7Aa/y8 ( Figure 1B) ------- COMMENT: c83c3de5e5b69049 10 aYGcHq/sVOUbgToscNg3znRGaFo ( Figure 1B) We found that virtually all rec12D cells (n = 240) eventually relo- calized Ark1 to the spindle (Figure 1B), indicating that the SAC ultimately becomes satisfied in achiasmate meiosis. ------- COMMENT: c83c3de5e5b69049 11 FWwaa8Xsh3fzqxg9lsZNaGxEGtw Figure 1C, 1D (comment: CHECK bipolar attachment of univalents) ------- COMMENT: c83c3de5e5b69049 12 GSDoKVHrCsiUO6zYP0Vg8a8VAAQ fig 1D ------- COMMENT: c83c3de5e5b69049 14 y1g01zxxdAUHesX8KQ8cx+vvlmw fig 1D although sister kinetochore split, segregation ends up mostly normal ------- COMMENT: c83c3de5e5b69049 15 GSDoKVHrCsiUO6zYP0Vg8a8VAAQ fig 1D ------- COMMENT: c83c3de5e5b69049 16 GSDoKVHrCsiUO6zYP0Vg8a8VAAQ fig 1D ------- COMMENT: c83c3de5e5b69049 17 lMC0/QqsVB1LZx6mP9hhwUxeBLA fig 1D although sister kinetochore sometimes split, segregation ends up mostly normal ------- COMMENT: c83c3de5e5b69049 18 GSDoKVHrCsiUO6zYP0Vg8a8VAAQ fig 1D ------- COMMENT: c83c3de5e5b69049 19 GSDoKVHrCsiUO6zYP0Vg8a8VAAQ fig 1D ------- COMMENT: c83c3de5e5b69049 21 y1g01zxxdAUHesX8KQ8cx+vvlmw fig 1D although sister kinetochore split, segregation ends up mostly normal ------- COMMENT: c83c3de5e5b69049 22 GSDoKVHrCsiUO6zYP0Vg8a8VAAQ fig 1D ------- COMMENT: c83c3de5e5b69049 23 F7m1lgbg4VqtrUNXnyMqyf+z6Nc ( Figure 1A) phase II (metaphase) was substantially extended ------- COMMENT: c83c3de5e5b69049 24 Hcj7HDj27fkU3cSmFfUsVz2BgNE ( Figure S3A) ------- COMMENT: c83c3de5e5b69049 25 GSDoKVHrCsiUO6zYP0Vg8a8VAAQ fig 1D ------- COMMENT: c83c3de5e5b69049 30 +t2qCCGlK6xrXs1Rvm4vTpyZddI Fig 4A ------- COMMENT: c83c3de5e5b69049 31 LJSsp/rwY9tEYj0LcB5R1yHR2+4 Fig 4B ------- COMMENT: c83c3de5e5b69049 33 iLGTaLF7H9Rzh+AgsaQ4DVw/zws (comment: CHECK >inc merotelic kinetochore attachment) ------- COMMENT: c83c3de5e5b69049 34 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: c83c3de5e5b69049 35 //QleQcD3kkWGdAJo2oN7LSuYAA fig 4B ------- COMMENT: c83c3de5e5b69049 40 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: c8412d1610d7fb9b 1 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: c8412d1610d7fb9b 2 DInHiJ49K5nhPa8pwBklm1Z9y9E Fig. 1A (comment: CenH) ------- COMMENT: c8412d1610d7fb9b 3 DInHiJ49K5nhPa8pwBklm1Z9y9E Fig. 1A (comment: CenH) ------- COMMENT: c8412d1610d7fb9b 4 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: c8412d1610d7fb9b 5 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: c8412d1610d7fb9b 6 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: c8412d1610d7fb9b 7 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: c8412d1610d7fb9b 8 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: c8412d1610d7fb9b 9 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: c8412d1610d7fb9b 10 jUK5ZZ/rQ14nmoA8BqUqolwNGFk (Fig. 2B) Immunoblotting assay showed that the protein level of the Rdp1 mutant (Rdp1D903A) is comparable to that of wild-type Rdp1, suggesting that the D903A mutation does not affect the stability of the mutant protein ------- COMMENT: c8412d1610d7fb9b 11 n1eCiF/2Pen5hWUFSmgGXRKdFr4 (Fig. 2C). (otr1R::ura4􏰃) (Fig. 2B) Immunoblotting assay showed that the protein level of the Rdp1 mutant (Rdp1D903A) is comparable to that of wild-type Rdp1, suggesting that the D903A mutation does not affect the stability of the mutant protein ------- COMMENT: c8412d1610d7fb9b 12 zjZZKAl4I9ZKZ9ZGgbNsLQCnN8U (Fig. 2D). cells localization of both methylated H3–K9 and Swi6 at centromeric otr1R::ura4􏰃 was severely affected in rdp1D903A cells (Fig. 2D). ------- COMMENT: c8412d1610d7fb9b 13 zjZZKAl4I9ZKZ9ZGgbNsLQCnN8U (Fig. 2D). cells localization of both methylated H3–K9 and Swi6 at centromeric otr1R::ura4􏰃 was severely affected in rdp1D903A cells (Fig. 2D). ------- COMMENT: c8412d1610d7fb9b 14 bQSUegys9CEp6Wbj3JroiFu2Gx8 hypersensitive to TBZ, indicating that chromosome segregation is not robust in these mutant cells (Fig. 3A). ------- COMMENT: c8412d1610d7fb9b 15 asV6VShOxdSf3mkZXvo+BDiURfU . We found that rdp1 mutants have a significantly higher percentage (􏰅20%) of cells with lagging chromosomes during late anaphase than in the wild-type strain (􏰆1%) (Fig. 3B) ------- COMMENT: c8412d1610d7fb9b 16 0ILABabjLhxjqPLXfuaAYH5GHbs (Fig. 3C and D) In contrast, a noticeably larger fraction of rdp1D903A cells exhibited an increased number of Swi6 foci, and most of these Swi6 foci still colocalized with Taz1, suggesting a declustering of telomeres even though the localization of telo- meres to the nuclear periphery was unaffected ------- COMMENT: c8412d1610d7fb9b 17 q7Y5/8gnOTElaJNtlgV0SjhrU/0 In contrast, a noticeably larger fraction of rdp1D903A cells exhibited an increased number of Swi6 foci, and most of these Swi6 foci still colocalized with Taz1, suggesting a declustering of telomeres even though the localization of telo- meres to the nuclear periphery was unaffected (Fig. 3 C and D) ------- COMMENT: c8412d1610d7fb9b 18 6XWjkdEL4IA5xbZK4WQJrO6sCss herefore, we conclude from these analyses that the RdRP activity of Rdp1 is essential for the generation of RITS-associated siRNAs. plus centrromeric chromatin assays ------- COMMENT: c8412d1610d7fb9b 20 q7Y5/8gnOTElaJNtlgV0SjhrU/0 In contrast, a noticeably larger fraction of rdp1D903A cells exhibited an increased number of Swi6 foci, and most of these Swi6 foci still colocalized with Taz1, suggesting a declustering of telomeres even though the localization of telo- meres to the nuclear periphery was unaffected (Fig. 3 C and D) ------- COMMENT: c8412d1610d7fb9b 21 TtmPOLrOCSzRO7ANi1pYOZ2WUgs (Fig. 4B). We found that, whereas siRNAs could be readily detected in the affinity-purified fraction of RITS from wild-type cells, there were no detectable RITS- associated siRNAs present in rdp1D903A, rdp1􏰄, or dcr1􏰄 cells ------- COMMENT: c8412d1610d7fb9b 22 TtmPOLrOCSzRO7ANi1pYOZ2WUgs (Fig. 4B). We found that, whereas siRNAs could be readily detected in the affinity-purified fraction of RITS from wild-type cells, there were no detectable RITS- associated siRNAs present in rdp1D903A, rdp1􏰄, or dcr1􏰄 cells ------- COMMENT: c8412d1610d7fb9b 23 TtmPOLrOCSzRO7ANi1pYOZ2WUgs (Fig. 4B). We found that, whereas siRNAs could be readily detected in the affinity-purified fraction of RITS from wild-type cells, there were no detectable RITS- associated siRNAs present in rdp1D903A, rdp1􏰄, or dcr1􏰄 cells ------- COMMENT: c8412d1610d7fb9b 24 WI/3wcw/zrH4TC9IeNJkd8AJLDE (Fig. 5A) Although all three components of RITS (Ago1, Chp1, and Tas3) are found to be dramatically enriched at otr1R::ura4􏰃 and centromeric repeats in wild-type cells, these proteins completely fail to localize to these centromeric loci in rdp1D903A cells ------- COMMENT: c8412d1610d7fb9b 25 WI/3wcw/zrH4TC9IeNJkd8AJLDE (Fig. 5A) Although all three components of RITS (Ago1, Chp1, and Tas3) are found to be dramatically enriched at otr1R::ura4􏰃 and centromeric repeats in wild-type cells, these proteins completely fail to localize to these centromeric loci in rdp1D903A cells ------- COMMENT: c8412d1610d7fb9b 26 WI/3wcw/zrH4TC9IeNJkd8AJLDE (Fig. 5A) Although all three components of RITS (Ago1, Chp1, and Tas3) are found to be dramatically enriched at otr1R::ura4􏰃 and centromeric repeats in wild-type cells, these proteins completely fail to localize to these centromeric loci in rdp1D903A cells ------- COMMENT: c8ccb11c47a11d42 1 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: c8ccb11c47a11d42 2 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: c8ccb11c47a11d42 3 NvmlkQuwX9T4dFwFcHia2Z/plXU The 210-nt region resides downstream of the distal poly(A) site and it is not included in the mature rrg1+ mRNA, in contrast to other regulatory elements for post-transcriptional control. However, some recent studies on S.pombe have shown that RNA pol II transcription proceeds beyond the poly(A) site and that the downstream sequences located in the 3′ noncoding region are responsible for transcription termination and mRNA 3′-end formation, which are closely coupled to efficient gene expression ------- COMMENT: c8ce525d900e703a 5 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: c8ce525d900e703a 6 idmfQtoLRetp/fFZlSK8sV/IyyI Fig 2, S1 ------- COMMENT: c8ce525d900e703a 7 JzyBKOIiyuH2U/GwaoWxC6/fJhQ Fig S2 ------- COMMENT: c8ce525d900e703a 8 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: c8ce525d900e703a 9 OD1pyANJIJ+L/uLd5YAg/RkHIyE (comment: 25 degrees C; using "low temperature" to distinguish from 30 degrees C;) Fig 1 ------- COMMENT: c8ce525d900e703a 10 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: c8ce525d900e703a 11 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: c8ce525d900e703a 12 OD1pyANJIJ+L/uLd5YAg/RkHIyE (comment: 25 degrees C; using "low temperature" to distinguish from 30 degrees C;) Fig 1 ------- COMMENT: c8ce525d900e703a 13 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: c8ce525d900e703a 14 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: c8ce525d900e703a 15 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: c8ce525d900e703a 16 OD1pyANJIJ+L/uLd5YAg/RkHIyE (comment: 25 degrees C; using "low temperature" to distinguish from 30 degrees C;) Fig 1 ------- COMMENT: c8ce525d900e703a 17 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: c8ce525d900e703a 18 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: c8ce525d900e703a 19 OD1pyANJIJ+L/uLd5YAg/RkHIyE (comment: 25 degrees C; using "low temperature" to distinguish from 30 degrees C;) Fig 1 ------- COMMENT: c8ce525d900e703a 20 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: c8ce525d900e703a 21 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: c8ce525d900e703a 22 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: c8ce525d900e703a 23 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: c8ce525d900e703a 24 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: c8ce525d900e703a 26 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: c8ce525d900e703a 27 D+ohyCgWQ0DtLhiBJvCnotYo49o Fig 5 ------- COMMENT: c8ce525d900e703a 28 /UfpBaJXlKkIDZwBxhCsPSg7ogc (comment: [vw added to cover missing EXP annotation based on localization phenotype below]) ------- COMMENT: c8e6356a39f3ee63 3 qMYQhSGOD0WIZOxtofqQXQwthwM (comment: assayed using RTS1 mut2 or mut8 on plasmid) ------- COMMENT: c8e6356a39f3ee63 4 qMYQhSGOD0WIZOxtofqQXQwthwM (comment: assayed using RTS1 mut2 or mut8 on plasmid) ------- COMMENT: c8e6356a39f3ee63 5 qMYQhSGOD0WIZOxtofqQXQwthwM (comment: assayed using RTS1 mut2 or mut8 on plasmid) ------- COMMENT: c8e6356a39f3ee63 10 qMYQhSGOD0WIZOxtofqQXQwthwM (comment: assayed using RTS1 mut2 or mut8 on plasmid) ------- COMMENT: c8e6356a39f3ee63 12 qMYQhSGOD0WIZOxtofqQXQwthwM (comment: assayed using RTS1 mut2 or mut8 on plasmid) ------- COMMENT: c8e6356a39f3ee63 13 qMYQhSGOD0WIZOxtofqQXQwthwM (comment: assayed using RTS1 mut2 or mut8 on plasmid) ------- COMMENT: c8f784dd2ebdf2e1 2 6Z8owWnO9LoCcc4N32KGDWQSTok Fig1A ------- COMMENT: c8f784dd2ebdf2e1 11 P5tvbmkwo6vASR9W2XOXxsDSQmk Fig3A In absence of rum1 cdc2-cdc13 kinase activity remains high in presence of P factor ------- COMMENT: c8f784dd2ebdf2e1 12 0T6eqFwpv3lIobPGer3XGxvx3/8 Fig3D loss of cig2 does not restore P factor induced G1 arrest ------- COMMENT: c8f784dd2ebdf2e1 16 ruBWiZtA6Teft0vvfVSZR+lBGBk Fig4 -(comment: sows proteasome involvment as well) ------- COMMENT: c8f784dd2ebdf2e1 18 s8zxygB4j9+cKrsniSSlYhY+9P0 Fig5A ------- COMMENT: c8f784dd2ebdf2e1 19 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: c8f784dd2ebdf2e1 21 REqA86wgEckE1+po1IZQ8wC5RUg Fig5C ------- COMMENT: c8f784dd2ebdf2e1 23 REqA86wgEckE1+po1IZQ8wC5RUg Fig5C ------- COMMENT: c8f784dd2ebdf2e1 26 REqA86wgEckE1+po1IZQ8wC5RUg Fig5C ------- COMMENT: c8f784dd2ebdf2e1 27 REqA86wgEckE1+po1IZQ8wC5RUg Fig5C ------- COMMENT: c8f784dd2ebdf2e1 30 VfXfr81MaQM2aWN8kcCFYFxfbTI Fig6A (comment: CHECK transcript assayed was Mat1-Mm) ------- COMMENT: c8f784dd2ebdf2e1 37 h09KQpN7u/q6m3yDchMr2e10lc8 Fig4 ------- COMMENT: c8f784dd2ebdf2e1 39 t4aWDllIe9Rh7+XFYg0ACgrd2m0 Fig2B after addition of P factor to nitrogen starved G1 arrested cells P factor does not further increase rum1 protein level. It is inferred that rum1 is required to maintain G1 arrest rather than bring it about. ------- COMMENT: c92c4fac293808ab 3 EfL4baZEhoZxxW503AyAxGAfZnU slightly more sensitive at low temperature than standard ------- COMMENT: c92c4fac293808ab 18 50W3jZMU2ksMuP8Ar/4fWOnooMk (comment: slightly worse than sfr1delta alone) ------- COMMENT: c92c4fac293808ab 50 DzEOL1y+7tER55y+AmQyogPKJvI (comment: during vegetative growth because non-sporulating strains used) ------- COMMENT: c92c4fac293808ab 51 DzEOL1y+7tER55y+AmQyogPKJvI (comment: during vegetative growth because non-sporulating strains used) ------- COMMENT: c92c4fac293808ab 52 DzEOL1y+7tER55y+AmQyogPKJvI (comment: during vegetative growth because non-sporulating strains used) ------- COMMENT: c92c4fac293808ab 53 DzEOL1y+7tER55y+AmQyogPKJvI (comment: during vegetative growth because non-sporulating strains used) ------- COMMENT: c92c4fac293808ab 54 DzEOL1y+7tER55y+AmQyogPKJvI (comment: during vegetative growth because non-sporulating strains used) ------- COMMENT: c9666a1ab5592282 6 Tu7/A/SYbNm4h7UEDJIwhlpHREM (comment: 2nd division) ------- COMMENT: c981881e2d6e2ab9 68 GfZ2pQlCpn45f0cCiGHyH1NqmX0 (comment: worse than either single mutant) ------- COMMENT: c981881e2d6e2ab9 69 GfZ2pQlCpn45f0cCiGHyH1NqmX0 (comment: worse than either single mutant) ------- COMMENT: c981881e2d6e2ab9 70 GfZ2pQlCpn45f0cCiGHyH1NqmX0 (comment: worse than either single mutant) ------- COMMENT: c981881e2d6e2ab9 71 GfZ2pQlCpn45f0cCiGHyH1NqmX0 (comment: worse than either single mutant) ------- COMMENT: c981881e2d6e2ab9 73 ycc/TGYKaQqAVbWsAiTjIyrLtOI (comment: same as mus81delta alone) ------- COMMENT: c981881e2d6e2ab9 74 ycc/TGYKaQqAVbWsAiTjIyrLtOI (comment: same as mus81delta alone) ------- COMMENT: c981881e2d6e2ab9 75 ycc/TGYKaQqAVbWsAiTjIyrLtOI (comment: same as mus81delta alone) ------- COMMENT: c981881e2d6e2ab9 76 ycc/TGYKaQqAVbWsAiTjIyrLtOI (comment: same as mus81delta alone) ------- COMMENT: c981881e2d6e2ab9 77 GfZ2pQlCpn45f0cCiGHyH1NqmX0 (comment: worse than either single mutant) ------- COMMENT: c981881e2d6e2ab9 78 GfZ2pQlCpn45f0cCiGHyH1NqmX0 (comment: worse than either single mutant) ------- COMMENT: c981881e2d6e2ab9 79 GfZ2pQlCpn45f0cCiGHyH1NqmX0 (comment: worse than either single mutant) ------- COMMENT: c981881e2d6e2ab9 80 GfZ2pQlCpn45f0cCiGHyH1NqmX0 (comment: worse than either single mutant) ------- COMMENT: c9eb9105f66cbab8 1 /GiDVlZsrOEYLxnuH39J0VNz7GQ (comment: well this is a slight fudge. The activity was assayed and present. We know this is glutamate dehydrogenase...so sone and will make "published") ------- COMMENT: c9f65982114faaaa 1 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: c9f65982114faaaa 2 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: c9f65982114faaaa 3 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: c9f65982114faaaa 4 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: c9f65982114faaaa 5 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: c9f65982114faaaa 6 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: c9f65982114faaaa 7 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: c9f65982114faaaa 8 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: c9f65982114faaaa 9 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: c9f65982114faaaa 10 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: c9f65982114faaaa 11 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: c9f65982114faaaa 12 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: c9f65982114faaaa 13 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: c9f65982114faaaa 17 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: c9f65982114faaaa 18 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: c9f65982114faaaa 19 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: c9f65982114faaaa 20 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: c9f65982114faaaa 21 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: c9f65982114faaaa 22 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: ca29b665ec8b2f94 2 3Da90xrxzZLoNcK8IqRDHi0MfNo During mitosis, tea1p persists at the cell tips, whereas for3p and bud6p leave the cell tip and accumulate more at the cell division plane. ------- COMMENT: ca29b665ec8b2f94 3 3Da90xrxzZLoNcK8IqRDHi0MfNo During mitosis, tea1p persists at the cell tips, whereas for3p and bud6p leave the cell tip and accumulate more at the cell division plane. ------- COMMENT: ca29b665ec8b2f94 27 SXswOzbvmsHw60Y/LbRHpYO66Js (comment: CHECK affecting FYPO:0004103) ------- COMMENT: ca29b665ec8b2f94 28 SXswOzbvmsHw60Y/LbRHpYO66Js (comment: CHECK affecting FYPO:0004103) ------- COMMENT: cafc26135a141916 1 T+Ffjl+MXaMC0ztO0y5b4XFRbXQ (comment: Y2H) fig 1 ------- COMMENT: cafc26135a141916 2 eZmebctUd0OA95JXIjcqR8qYZcI fig1 (comment: only bqt1 is fused to the activation domain (that's why I am not adding this function to bqt2)) ------- COMMENT: cafc26135a141916 3 EuhArqRsQzbbtdLoKVrgkCbdk9k (comment: CHECK bqt1 is fused to the activation domain) ------- COMMENT: cafc26135a141916 5 kCUZlgwtD01tlMJmicXZ6Ijhtlg fig1 (comment: Y2H) ------- COMMENT: cafc26135a141916 6 kCUZlgwtD01tlMJmicXZ6Ijhtlg fig1 (comment: Y2H) ------- COMMENT: cb1516626fc1f680 1 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: cb1516626fc1f680 2 C3evxWzsaJkiOIb9jRgVjpvYJnY Figure 4D ------- COMMENT: cb1516626fc1f680 3 abUGseCzXjmoOrtkSXH9Wxhfm8c fig 3a ------- COMMENT: cb1516626fc1f680 4 nnuWhrFU7jaQ2CsXT4A21I2oLbM fig 3a ------- COMMENT: cb1516626fc1f680 5 a2ZFVx6Mzp8sNPP6z5EA3vUonf4 Fig. 3A, B present in interphase cells ------- COMMENT: cb1516626fc1f680 6 IjizA2X1zltJErmAD8vFW1EBrqs Fig. 3A, B present in interphase ------- COMMENT: cb1516626fc1f680 7 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: cb1516626fc1f680 8 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: cb1516626fc1f680 9 3EfvjaMCp77T+8C8sptTG84+fIc Fig1B ------- COMMENT: cb1516626fc1f680 10 t4msNwd2fonJUQE6JRh6f0ZGkr8 Fig1b ------- COMMENT: cb1516626fc1f680 11 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: cb1516626fc1f680 12 8dk6bSVTKpYOr6w4X4gysEsXgSU Fig S1G ------- COMMENT: cb1516626fc1f680 13 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: cb1516626fc1f680 14 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: cb1516626fc1f680 15 urOpmJ7V2cOTeJDX6yi2ZCNyVFA Fig 4D ------- COMMENT: cb1516626fc1f680 16 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: cb1516626fc1f680 17 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: cb1516626fc1f680 18 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: cb1516626fc1f680 19 pwcwASlWQtRBBoormzj5gHYObdE fig 4A ------- COMMENT: cb1516626fc1f680 20 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: cb1516626fc1f680 21 xUY4puK+2bmL+i2aZgbTO2W8o/4 (comment: synchronous mitotic cells) fig 1c ------- COMMENT: cb1516626fc1f680 22 /TVuKZ/fweW9VHrSTG3yYQbZrvc Fig. 1C (comment: synchronous mitotic cells) ------- COMMENT: cb1516626fc1f680 28 RKmt1omZhmNVZo4JeYOrBugdBI0 figure 3A ------- COMMENT: cb1516626fc1f680 29 RKmt1omZhmNVZo4JeYOrBugdBI0 figure 3A ------- COMMENT: cb1516626fc1f680 30 RI+0xv5mUL8+DZt4nmUoG0x32gI figure 4C. (comment: synchronous mitotic cells) ------- COMMENT: cb1516626fc1f680 31 sT7Q8AXtl4TfilsHSfnsmkDFdgs Fig. S1h ------- COMMENT: cb1516626fc1f680 32 aIdD3pPNb0+deHoaJ3p0FDOpQ8Q (Figure 1F ------- COMMENT: cb1516626fc1f680 33 6UbAvkYdqOeDQaOxKAwnbqKO504 Figures 1F, S1H). ------- COMMENT: cb1516626fc1f680 35 KfDk8D+77WXt2X5nWkop257gFtY figure 4D. s ------- COMMENT: cb1516626fc1f680 36 6k0Qunv3iIlADjLG6yiFcJpA+q8 Figure S1e ------- COMMENT: cb1516626fc1f680 37 joC6jJccwRwyV1DTiwAjoKSRPSQ Fig S3B ------- COMMENT: cb1516626fc1f680 38 4O8xcHisKrNN/tgvTKDomttTtoU Figure S3B ------- COMMENT: cb1516626fc1f680 39 4O8xcHisKrNN/tgvTKDomttTtoU Figure S3B ------- COMMENT: cb1516626fc1f680 40 4O8xcHisKrNN/tgvTKDomttTtoU Figure S3B ------- COMMENT: cb1516626fc1f680 41 4O8xcHisKrNN/tgvTKDomttTtoU Figure S3B ------- COMMENT: cb1516626fc1f680 42 4O8xcHisKrNN/tgvTKDomttTtoU Figure S3B ------- COMMENT: cb1516626fc1f680 43 4O8xcHisKrNN/tgvTKDomttTtoU Figure S3B ------- COMMENT: cb1516626fc1f680 44 0YGqnvFEFBY2YfZ72mjyXJkz/Go Fig. 2C, D, S2B, ------- COMMENT: cb1516626fc1f680 45 0YGqnvFEFBY2YfZ72mjyXJkz/Go Fig. 2C, D, S2B, ------- COMMENT: cb1516626fc1f680 46 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: cb15339cd6cc0df8 1 4dMSNns3D+Ta9zfS6gCRrIf9S+o (comment: competatively with cofilin) ------- COMMENT: cb15339cd6cc0df8 2 Md2u2AvbJamms1DnjTMl4CFVbfc SpAip1 dramatically stimulated severing by 100 nM cofilin, with a maximum rate at 1.5 􏰋M SpAip1 and lower rates at higher concentrations (Fig. 3C).At all SpAip1 concentrations tested 􏰅80% of new barbed ends created by severing events depolymerized (Fig. 3D) at rates that decreased insignificantly with SpAip1 concentration (Fig. 3E). These depolymerization rates were higher than published values (2), likely because severing near barbed ends was difficult to distin- guish from filament depolymerization. ------- COMMENT: cb15339cd6cc0df8 15 7YxczmbfAOgmKH6Yu7VmYDkjYWQ The fission yeast lacking Aip1 have normal appearing actin patches, cables, and contractile rings (Fig. 5B). ------- COMMENT: cb15339cd6cc0df8 17 pqTw8oV12ajhRYsxULnbWW/WLnA Figs. 6B and C, Table 3 ------- COMMENT: cb15339cd6cc0df8 19 3sn5qmMU1NADwLaIGNWuEgJ6sxY Surprisingly, contractile rings began to constrict earlier in 􏰀aip1 cells than wild type cells (Fig. 7, A and B, and Table 3), foreshortening the maturation period before constriction ------- COMMENT: cb15339cd6cc0df8 20 Yml5vEZToLUm0dpFjASDjWCfZn0 As a result, ring constriction in 􏰀aip1 cells takes 􏰅 5 min less than in wild type cells (Table 3). Overall, cytokinesis was 􏰅16 min (24%) faster in 􏰀aip1 cells than wild type cells, because the maturation period was shorter and the rings constricted faster. ------- COMMENT: cb309c1fc155632b 3 lAjRoAewRQ0e2hd8Vt/rPmhP+/w (comment: CHECK moved down from GO:0016706 30/8/2014 . activated_by(CHEBI:29033)) ------- COMMENT: cb3e2a895f69824b 1 uPMFmp3LT0Xum1QdT96xvCwIWUk (comment: CHECK phenotype suppressed by Scd1-3GFP Pak1-GBP (Scd1-Pak1 bridge) expression) ------- COMMENT: cb3e2a895f69824b 2 XY6DO+o4Iz/fxgbZJsWOpQn5yWU (comment: CHECK lethality suppressed by expression of Scd1-3GFP and Pak1-GBP (Scd1-Pak1 bridge)) ------- COMMENT: cb3e2a895f69824b 3 O8UcX9oFBF+fmJhBfQ4Icir0Dc0 No Scd1 recruitment to cell cortex Low levels of active Cdc42 ------- COMMENT: cb3e2a895f69824b 4 vvKDzKeXGA0sBMOpmCd5MgdxUYs (comment: Similar to scd2∆ ras1∆.) No Scd1 recruitment to cell cortex. ------- COMMENT: cb3e2a895f69824b 5 BlvG1PplP2AHDXNEuoT++KhTaYQ (comment: CHECK Lethality suppressed by the expression of Scd1-3GFP and Pak1-GBP (Scd1-Pak1 bridge)) ------- COMMENT: cb3e2a895f69824b 7 0uP0FahcHYN4oFODG50Q9b2g1w4 Decreased levels of Scd1 at the cell cortex ------- COMMENT: cb3e2a895f69824b 8 0uP0FahcHYN4oFODG50Q9b2g1w4 Decreased levels of Scd1 at the cell cortex ------- COMMENT: cb3e2a895f69824b 9 0uP0FahcHYN4oFODG50Q9b2g1w4 Decreased levels of Scd1 at the cell cortex ------- COMMENT: cb56c06e21e64b64 2 3yqq8mT6r2p3L3RL/sFe2Tak0os fig2A ------- COMMENT: cb56c06e21e64b64 3 gbsEZDL2WsTbMBJ8nTkh0vGNYlA fig 2A ------- COMMENT: cb56c06e21e64b64 4 gbsEZDL2WsTbMBJ8nTkh0vGNYlA fig 2A ------- COMMENT: cb56c06e21e64b64 5 gbsEZDL2WsTbMBJ8nTkh0vGNYlA fig 2A ------- COMMENT: cb56c06e21e64b64 6 Krt8IEN2thpV+MLPwLEVMzonARU fig2B ------- COMMENT: cb56c06e21e64b64 7 Krt8IEN2thpV+MLPwLEVMzonARU fig2B ------- COMMENT: cb56c06e21e64b64 8 Krt8IEN2thpV+MLPwLEVMzonARU fig2B ------- COMMENT: cb56c06e21e64b64 9 Krt8IEN2thpV+MLPwLEVMzonARU fig2B ------- COMMENT: cb56c06e21e64b64 10 Krt8IEN2thpV+MLPwLEVMzonARU fig2B ------- COMMENT: cb56c06e21e64b64 13 s7cvhfiYiveaAA2KzWLwMqq+fAs fig 4c ------- COMMENT: cb56c06e21e64b64 14 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: cb8f7133d0ae3b28 1 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: cb8f7133d0ae3b28 2 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: cb8f7133d0ae3b28 3 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: cb8f7133d0ae3b28 4 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: cb8f7133d0ae3b28 5 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: cb8f7133d0ae3b28 6 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: cb8f7133d0ae3b28 7 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: cb8f7133d0ae3b28 8 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: cb8f7133d0ae3b28 9 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: cb8f7133d0ae3b28 10 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: cb8f7133d0ae3b28 11 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: cb8f7133d0ae3b28 12 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: cb8f7133d0ae3b28 13 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: cb8f7133d0ae3b28 14 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: cb8f7133d0ae3b28 15 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: cb8f7133d0ae3b28 16 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: cb8f7133d0ae3b28 17 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: cb8f7133d0ae3b28 18 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: cb8f7133d0ae3b28 19 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: cb8f7133d0ae3b28 20 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: cb8f7133d0ae3b28 21 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: cb8f7133d0ae3b28 22 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: cb8f7133d0ae3b28 23 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: cb8f7133d0ae3b28 24 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: cbaaa61496b71364 7 bAykROnIk+3JsPpo8I+8tJ3PXqY truncated Gar2 accumulates in this dense body ------- COMMENT: cbc863c3c9dd38e5 1 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: cbc863c3c9dd38e5 2 BHQfyV1qRwe83/WHjKPtBeG1doM fig 2a (comment: CHECK WT 10% @36degrees) ------- COMMENT: cbc863c3c9dd38e5 3 4yacSWX1HUlpibLrHaGjlMPHIJM figure 2B, C ------- COMMENT: cbc863c3c9dd38e5 4 v1zR0ETyaOby9jj/mDo1M95M7kE fig 3A ------- COMMENT: cbc863c3c9dd38e5 5 v1zR0ETyaOby9jj/mDo1M95M7kE fig 3A ------- COMMENT: cbc863c3c9dd38e5 6 v1zR0ETyaOby9jj/mDo1M95M7kE fig 3A ------- COMMENT: cbc863c3c9dd38e5 7 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: cbc863c3c9dd38e5 8 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: cbc863c3c9dd38e5 9 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: cbc863c3c9dd38e5 10 NRpakGQuKoFHqeG1+MyKlxvUPVA fig 5, (comment: 6 min late) ------- COMMENT: cbc863c3c9dd38e5 12 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: cbc863c3c9dd38e5 13 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: cbc863c3c9dd38e5 14 EK3cJaIpApHaZfDj/il8fbVV8BY fig S2 ------- COMMENT: cbe3e38931547a9c 1 xJ2kPgXfybPA1GXdSd5ZJ0O+RGg (comment: CHECK NORMAL LENGTH) ------- COMMENT: cbe3e38931547a9c 6 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: cc3251094baf47d8 1 IaP4E6AMTZTORu+Bm0fvWurr7d0 Arginine, glutamic acid, or proline weakly support growth with an ammonium nitrogen source. The mutant grows normally when these amino acids are used as the sole nitrogen source. The mutant cannot utilize aspartic acid and asparagine as the sole nitrogen source. ------- COMMENT: cc3251094baf47d8 2 LbPQSim4xGr+XSpMgasDUt70ZJY yhm2+ works as a dosage suppressor of auxotrophic growth defect in the maa1 (glu1) mutant. ------- COMMENT: cc3251094baf47d8 3 1H9YC5kNq+QvMAjGwG5CsP6PClE Interestingly, glutamine efficiently rescues the growth defect of the glu1 mutant compared to glutamate, even in the presence of ammonium (Figure 1F, Barel and MacDonald 1993). ------- COMMENT: cc3251094baf47d8 4 3d8pdCXGDWorbg1K4tTfPo1Zcnw The glu1 mutant grew when glutamate was used as the sole nitrogen source, although glutamate poorly supported its growth in the presence of ammonium (Figures 1B and F). ( In general, their availability is regulated by nitrogen catabolite repression; thus, it is greatly affected by the presence of ammonium, a high-quality nitrogen source.) ------- COMMENT: cc3251094baf47d8 5 OqEHNlWthmFscJsubIqsjO4XMOw It catalyzes the transamination between oxaloacetate and glutamate, leading to the formation of aspartate and 2-oxoglutarate (Figure 1A). phenotypes support glutamate production. evidence also supports that this ebzyme would normally be other direction? on rich nitrogen source ------- COMMENT: cc3251094baf47d8 6 iCcwLskN30GOFjdmoaHQ4vEvzRg The glu1-NS176 mutation was rescued by sup3-5 (Figure 1E), a tRNASer (SPATRNASER.03) suppressor mutation that suppresses the TGAopal nonsense codon (Hottinger et al., 1982, Niwa et al., 1989). This is direct proof that the observed growth defect in the ammonium medium is caused by the maa1 gene nonsense mutation in the glu1- NS176 strain. ------- COMMENT: cc3251094baf47d8 7 zvn3rwjHOaVXH2yDb6rPXz3JgxI In addition to glu1/maa1, the yhm2 gene (SPBC83.13) is a multicopy weak suppressor of the growth defect in the glu1-NS176 mutant. The glu1 mutant harboring the multicopy yhm2+ plasmid grew slower than the glu1-corrected, wild-type strain (maa1+ (integrated); Figure 1B). The Yhm2 homolog in Saccharomyces cerevisiae is a mitochondrial carrier protein that imports 2-oxoglutarate into the mitochondria (Castegna et al., 2010). Therefore, increasing the 2-oxoglutarate concentration in mitochondria may alleviate the ammonium utilization defect in the glu1-NS176 mutant. The yhm2∆ strain showed no growth defects under all medium conditions examined, whereas the glu1 and yhm2∆ mutations had an additive effect. The glu1-NS176 yhm2∆ double mutant grew slower than each single mutant in yeast extract medium (Figure 1F). The S. pombe glu1-NS176 yhm2∆ double mutant grew when amino acids, except aspartate and asparagine, were used as the sole nitrogen source (Figure 1F). Interestingly, all the tested amino acids, including glutamine, did not support the growth of double mutant in the presence of ammonium. S. cerevisiae mutants, which lack all mitochondrial 2-oxoglutarate carriers including Yhm2, cannot grow when ammonium is the sole nitrogen source, and importantly, this growth defect is rescued by the addition of glutamate (Palmieri et al., 2001, Castegna et al., 2010, Scarcia et al., 2017). ------- COMMENT: cc42fec3996fecfa 17 ZAT0hsdUBE6Y5e26G20G9akTLO8 (comment: non-flocculating cells) ------- COMMENT: cd0646514a96db42 2 rNAsgHwTIF7FxF6w+e0Rp1utd7o figure 1D. ------- COMMENT: cd0646514a96db42 7 IZz0OkwHlAtDME0Qv5C1lq9yiTs Dma1 ubiquitinates Tip1 ------- COMMENT: cd0646514a96db42 19 VhfTg6ItU1b+UIWLHvI+LrS2Acw More interestingly, in the presence of HU, the binding between Tea4 and Tip1 was elevated in dma1Δ cells and in wild-type cells treated with deubiquitinating enzyme USP2 to remove Tip1 ubi- quitination (Fig. 4f), this is consistent with increased polar growth in dma1Δ cells (Fig. 4b). ------- COMMENT: cd0646514a96db42 22 bNgh/Z8oRphByXjS0l2LwW4RDGE Strikingly, Tip1 ubiquitination was abolished in both dma1 mutants (Fig. 2d), demonstrating that both functional FHA and RF domains in Dma1 are required for Tip1 ubiquitination in vivo. ------- COMMENT: cd0646514a96db42 23 pZAHxbp9YLmjh5NWTWZt/NPnaBM (Fig. 2d) ------- COMMENT: cd0646514a96db42 24 YXHGFHU5LadekPlbrwHvPt7VWDU (Fig. 4) ------- COMMENT: cd0646514a96db42 25 YXHGFHU5LadekPlbrwHvPt7VWDU (Fig. 4) ------- COMMENT: cd0646514a96db42 26 n9FB392w4AX4Opw+OmkG617200E though HU treatment caused equally efficient arrest at S phase in both wild-type and dma1Δ cells (Supplementary Fig. 4) ------- COMMENT: cd0646514a96db42 28 dHpKYqIh9vGqqtWfycKuhEoWVQw We found that Tip1 was efficiently ubiquitinated in the absence of the calcineurin catalytic subunit Ppb1, with a similar degree of modification to that of wild-type cells (Fig. 6a). ------- COMMENT: cd18b7b226e58591 5 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: cd18b7b226e58591 7 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: cd18b7b226e58591 8 xNFMkKKBAWIS2tzOEYaO+t5BS5k Fig. S2) ------- COMMENT: cd18b7b226e58591 16 QFkCESTrJhb/SL2aDPixk6tlgxU (comment: Pho8Δ60 assay) (Fig. S3A). ------- COMMENT: cd18b7b226e58591 23 xNFMkKKBAWIS2tzOEYaO+t5BS5k Fig. S2) ------- COMMENT: cd18b7b226e58591 38 4AxEkBEg5pmoNB7gyuYV/qpDL2s In contrast, Pho8Δ60 activity was only restored to about half of the wild-type level when expressing Atg38[AIM mut]. AND Tdh1-YFP processing assay ------- COMMENT: cd18b7b226e58591 39 bxEgXl6Fo3Be/ltsfCHN6tT0/J4 Figure 3A, B ------- COMMENT: cd18b7b226e58591 51 BaHmeCDoTtpcr4+QCU3ZVPgzM3o Figure 2A (comment: Pho8Δ60 autophagy assay) ------- COMMENT: cd18b7b226e58591 56 QFkCESTrJhb/SL2aDPixk6tlgxU (comment: Pho8Δ60 assay) (Fig. S3A). ------- COMMENT: cd18b7b226e58591 58 RqjHmt6Sv/pnSkR62S5BRwAL3I4 Fig. S1F ------- COMMENT: cd18b7b226e58591 59 RqjHmt6Sv/pnSkR62S5BRwAL3I4 Fig. S1F ------- COMMENT: cd18b7b226e58591 62 /+WS65tc/DD30n7xs9eLKKrO5oQ Fig. S1E ------- COMMENT: cd18b7b226e58591 69 FeX3nNhC/gudGSWvsa359Zi1Hgk Fig. S1D ------- COMMENT: cd18b7b226e58591 95 aOqYL7F2KV5d9E4mVC2uoiGmluk Figure 1E ------- COMMENT: cd18b7b226e58591 96 nUCcEDoowWdBc8w5z3A4wbzAAHc Figure 1F ------- COMMENT: cd18b7b226e58591 97 OxhXsHTWPMTWMSNHZVVAR/SH7Qk Figure 1G) ------- COMMENT: cd18b7b226e58591 98 vR/3Xi4QGY5MdXBEKAx8xZE44AQ Figure 3A, B; ut reduced the PAS accumulation of the ...t Atg14, ...s Atg18b and Atg24b, Atg2, Atg5, Atg16, and Atg8 ------- COMMENT: cd18b7b226e58591 99 vR/3Xi4QGY5MdXBEKAx8xZE44AQ Figure 3A, B; ut reduced the PAS accumulation of the ...t Atg14, ...s Atg18b and Atg24b, Atg2, Atg5, Atg16, and Atg8 ------- COMMENT: cd18b7b226e58591 100 vR/3Xi4QGY5MdXBEKAx8xZE44AQ Figure 3A, B; ut reduced the PAS accumulation of the ...t Atg14, ...s Atg18b and Atg24b, Atg2, Atg5, Atg16, and Atg8 ------- COMMENT: cd18b7b226e58591 101 vR/3Xi4QGY5MdXBEKAx8xZE44AQ Figure 3A, B; ut reduced the PAS accumulation of the ...t Atg14, ...s Atg18b and Atg24b, Atg2, Atg5, Atg16, and Atg8 ------- COMMENT: cd18b7b226e58591 102 vR/3Xi4QGY5MdXBEKAx8xZE44AQ Figure 3A, B; ut reduced the PAS accumulation of the ...t Atg14, ...s Atg18b and Atg24b, Atg2, Atg5, Atg16, and Atg8 ------- COMMENT: cd18b7b226e58591 103 vR/3Xi4QGY5MdXBEKAx8xZE44AQ Figure 3A, B; ut reduced the PAS accumulation of the ...t Atg14, ...s Atg18b and Atg24b, Atg2, Atg5, Atg16, and Atg8 ------- COMMENT: cd18b7b226e58591 104 OxhXsHTWPMTWMSNHZVVAR/SH7Qk Figure 1G) ------- COMMENT: cd18b7b226e58591 105 OxhXsHTWPMTWMSNHZVVAR/SH7Qk Figure 1G) ------- COMMENT: cd18b7b226e58591 106 OxhXsHTWPMTWMSNHZVVAR/SH7Qk Figure 1G) ------- COMMENT: cd18b7b226e58591 107 OxhXsHTWPMTWMSNHZVVAR/SH7Qk Figure 1G) ------- COMMENT: cd48646c620974e6 1 6MoW5Z9vDmc3+MsforXTRmllptY Sid2 kinase phosphorylates Klp2 on serine residues 113 and 123 based on both in vitro and in vivo evidence. Phosphorylation on these residues disrupts interaction of Klp2 with Mal3. ------- COMMENT: cd90915e77803d7f 11 JUtr7cCb7X5ejoIj+bwMk6Qu9X0 (comment: distributive; substrate preference: small gaps with a 5′-phosphate group) ------- COMMENT: cd90915e77803d7f 16 jgxL4UYIIvC1xQ8RvE1iSdwoJ1I (comment: can incorporate NTPs or dNTPs; changed from primase activity because not tested with unprimed template) ------- COMMENT: cd959543ef54eb92 1 VVZWxkFgJ/AwnIvzPgQ043CsZtI (comment: CHECK abolished homodimerization) Fig. 6, ------- COMMENT: cd959543ef54eb92 2 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: cd959543ef54eb92 4 IUO99Np9GK9dwKnaq1mu0dH6Ozs fig 7 (comment: sdj mutant is unstable) ------- COMMENT: cd959543ef54eb92 5 IUO99Np9GK9dwKnaq1mu0dH6Ozs fig 7 (comment: sdj mutant is unstable) ------- COMMENT: ce16e022a8aca226 13 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: ce16e022a8aca226 82 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: ce16e022a8aca226 83 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: ce1fc9c90e999884 1 ZWKQI9qgwBvYgV3mD3cdpWHJZ4Y Fig 1 (comment: live imaging of Tip1YFP and CFP tubulin) ------- COMMENT: ce1fc9c90e999884 2 6EJ4GZBN4CfRoh1DBJQX4uWBLdA Fig 2 (comment: CHECK same phenotype as tea2delta and tip1delta single mutants) ------- COMMENT: ce1fc9c90e999884 3 6EJ4GZBN4CfRoh1DBJQX4uWBLdA Fig 2 (comment: CHECK same phenotype as tea2delta and tip1delta single mutants) ------- COMMENT: ce1fc9c90e999884 4 6EJ4GZBN4CfRoh1DBJQX4uWBLdA Fig 2 (comment: CHECK same phenotype as tea2delta and tip1delta single mutants) ------- COMMENT: ce1fc9c90e999884 5 jBgzJNNvRsoeNnnOZpFg52VnXRo co-localises with tip1 ------- COMMENT: ce1fc9c90e999884 6 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: ce1fc9c90e999884 7 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: ce1fc9c90e999884 8 DG2W8eHvSDYPNhDsj7t7E575dOA Fig 4A, B ------- COMMENT: ce1fc9c90e999884 9 DG2W8eHvSDYPNhDsj7t7E575dOA Fig 4A, B ------- COMMENT: ce1fc9c90e999884 10 mFDDf9aTydCLwlnxSYrFs9kSamM Fig 4E-H Tip1YFP is expressed from endogenous tip1 gene tagged with YFP ------- COMMENT: ce1fc9c90e999884 11 YUVPFU/st+BZXPEXI6S27Ys8LUk Fig 4C Tip1YFP is expressed from endogenous tip1 gene tagged with YFP ------- COMMENT: ce1fc9c90e999884 14 5zLBcPfE96G7rFQPJBZsaEOa1Og Fig 4C ------- COMMENT: ce1fc9c90e999884 15 rqDi4XBN6mbRnpGi+B6FnzKf6MY Fig 4E-H ------- COMMENT: ce1fc9c90e999884 16 q8vsZxdPnK09rpVKLHRLl2b7Fjw Fig 4I ------- COMMENT: ce1fc9c90e999884 17 wA2zDi2ljZyRdZxkVyg7LW8joIY Fig 5C ------- COMMENT: ce1fc9c90e999884 18 GSI0UOpB/GWNeBkwLbrFrUXRZkc Fig 5A, B (comment: Endogenous tea2 tagged with GFP) ------- COMMENT: ce1fc9c90e999884 19 GSI0UOpB/GWNeBkwLbrFrUXRZkc Fig 5A, B (comment: Endogenous tea2 tagged with GFP) ------- COMMENT: ce1fc9c90e999884 20 GSI0UOpB/GWNeBkwLbrFrUXRZkc Fig 5A, B (comment: Endogenous tea2 tagged with GFP) ------- COMMENT: ce1fc9c90e999884 21 4U71rQFh82wvB5d5Lmk+UrCODjs Fig 5E, F (comment: CHECK tea2-GFP is mildly overexpressed from the repressed integrated nmt1 promoter) ------- COMMENT: ce1fc9c90e999884 24 cejqT17FtaDYnMg52LGBKQuDi3o Fig 5E, F (comment: CHECK tea2-GFP is mildly overexpressed from the repressed integrated nmt1 promoter) ------- COMMENT: ce1fc9c90e999884 27 ZNMUWRP2MgjRiZvNGT7fcmfCkmc (comment: plus end directed) ------- COMMENT: ce1fc9c90e999884 28 38lkpu+1UYNBPLsvW09LKBxUFiY Fig 6A, B (comment: CHECK GFPmal3 is mildly overexpressed from the repressed nmt1 promoter) ------- COMMENT: ce1fc9c90e999884 29 LwTXHfA7iLI5U8wSgEvYAEMeTiI Fig 6C, D (comment: CHECK GFPmal3 is mildly overexpressed from the repressed nmt1 promoter) ------- COMMENT: ce1fc9c90e999884 30 LRpuGvZsQNdt/Xp9OYmoYaqZEMo Fig6E ------- COMMENT: ce1fc9c90e999884 31 liz7MErC7d1Mu36fi2rldELhoJY Fig6E ------- COMMENT: ce1fc9c90e999884 33 tHZednPCYX4IuB06G96Kk763STo colocalises with tip1 ------- COMMENT: ce1fc9c90e999884 34 6EJ4GZBN4CfRoh1DBJQX4uWBLdA Fig 2 (comment: CHECK same phenotype as tea2delta and tip1delta single mutants) ------- COMMENT: ce612cb3c6fbd641 6 9zOfwWc6p512HuJ+UWGn30amXn4 fig 2H, (comment: cell free system) ------- COMMENT: ce612cb3c6fbd641 7 9zOfwWc6p512HuJ+UWGn30amXn4 fig 2H, (comment: cell free system) ------- COMMENT: ce612cb3c6fbd641 8 9zOfwWc6p512HuJ+UWGn30amXn4 fig 2H, (comment: cell free system) ------- COMMENT: ce612cb3c6fbd641 9 9zOfwWc6p512HuJ+UWGn30amXn4 fig 2H, (comment: cell free system) ------- COMMENT: ce612cb3c6fbd641 10 9zOfwWc6p512HuJ+UWGn30amXn4 fig 2H, (comment: cell free system) ------- COMMENT: ce612cb3c6fbd641 11 a16KNEiHoQ+gU5j1GMcASpsBV/M fig. 2i ------- COMMENT: ce612cb3c6fbd641 12 a16KNEiHoQ+gU5j1GMcASpsBV/M fig. 2i ------- COMMENT: ce612cb3c6fbd641 13 a16KNEiHoQ+gU5j1GMcASpsBV/M fig. 2i ------- COMMENT: ce69bd1defde2d3e 1 8vOYmGfEquwPloXIktjDELwilZM mei4-N136A combined with pat1-114 was efficiently blocked at the meiosis I onset with telomere clustered at SPBs (in the bouquet configuration) at 32˚C, which arrest can be released by a temperature shift down to 25˚C. ------- COMMENT: ce69bd1defde2d3e 4 8vOYmGfEquwPloXIktjDELwilZM mei4-N136A combined with pat1-114 was efficiently blocked at the meiosis I onset with telomere clustered at SPBs (in the bouquet configuration) at 32˚C, which arrest can be released by a temperature shift down to 25˚C. ------- COMMENT: ce86e0783b08ae9a 8 ScznxcKy7rzKCkNF/D7HtTKtmA0 Eisosomes protruding towards cell interior ------- COMMENT: ce86e0783b08ae9a 9 AcutVxDv78egBv6nLn3y1KnLW98 Sterols do not accumulate in endosomes ------- COMMENT: ce86e0783b08ae9a 25 474D4LhfSYKehBL1XRRDCswf+nQ Sterols do not accumulate in endosomes after treatement with CK-666 ------- COMMENT: ce86e0783b08ae9a 27 474D4LhfSYKehBL1XRRDCswf+nQ Sterols do not accumulate in endosomes after treatement with CK-666 ------- COMMENT: ce86e0783b08ae9a 28 p/xE61xRyI4C3TJ869w/DzKPRKs Sterols accumulate in endosomes ------- COMMENT: ce86e0783b08ae9a 30 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: ce86e0783b08ae9a 31 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: ce86e0783b08ae9a 32 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: ce86e0783b08ae9a 33 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: ce86e0783b08ae9a 34 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: ce86e0783b08ae9a 35 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: ce86e0783b08ae9a 36 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: ce86e0783b08ae9a 37 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: ce86e0783b08ae9a 38 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: ce86e0783b08ae9a 40 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: ce86e0783b08ae9a 41 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: ce86e0783b08ae9a 42 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: ce86e0783b08ae9a 43 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: ce86e0783b08ae9a 44 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: ce86e0783b08ae9a 46 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: ce86e0783b08ae9a 47 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: ce86e0783b08ae9a 48 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: ce86e0783b08ae9a 49 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: ce86e0783b08ae9a 50 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: ce86e0783b08ae9a 51 yqIotQM0YG6kTttSIxnZZHX++xs (comment: Evaluated with D4H sterol sensor; internal structures) ------- COMMENT: ce86e0783b08ae9a 52 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: ce86e0783b08ae9a 53 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: ce86e0783b08ae9a 54 cMG0EQdZZbdOrjkN9m7IJB/RXCY (comment: Evaluated with D4H sterol sensor) ------- COMMENT: cea510a27fb48927 10 oaVMQZFLnhsJzMQ85/fQD4MKl8o (comment: CHECK in vitro assay with purified proteins) ------- COMMENT: ceb4bd4bfdcc4dae 1 SUPJ1iqMOEwajzytH+FIl2Z6N6k fig 2B (comment: in vitro binding assay with Cdc15 F-BAR domain and Cdc12 peptide aa20-40) ------- COMMENT: ceb4bd4bfdcc4dae 2 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: ceb4bd4bfdcc4dae 3 59YHQXZhRbKGVyry1nGm4QbMqVU (comment: additional cewlll. poles) ------- COMMENT: ceb4bd4bfdcc4dae 5 omPbYnUTkBrJLIvpMSC8fbkAIOA Figures 3C, 3D ------- COMMENT: ceb4bd4bfdcc4dae 11 oFUwi983uXUrX8jBF9ktIFW/dOg Pxl1 direct interaction with Cdc15 F-BAR domain ------- COMMENT: ceb4bd4bfdcc4dae 12 Yh2ihWmO37OLAIlRBrR94qp4cTw (comment: In vitro binding assay with Cdc15 F-BAR domain and full length Pxl1) ------- COMMENT: ceb4bd4bfdcc4dae 13 5IGc3difXx4+SJNj9Vf3lGyEbhs Figure 4F ------- COMMENT: ceb4bd4bfdcc4dae 14 L2Ieknugoxj0KSDNS5eEIDP9T+o Figure S2B of purified Cdc15 F-BAR domain ------- COMMENT: ceb4bd4bfdcc4dae 15 5SLq4jjUZx4o2fqO4NJ9786gpN0 Figure S2C ------- COMMENT: ceb4bd4bfdcc4dae 16 kvCFqixugIv44oEmCyyRC3DF+/I (comment: moved down from abnormal localization) ------- COMMENT: ceb4bd4bfdcc4dae 17 OE5uE9qpj9mt815ae2GM4ctd5G0 These results indicate that the Cdc15 F-BAR domain can position Cdc12 directly at the PM by binding membrane and Cdc12 simultaneously. ------- COMMENT: ceb4bd4bfdcc4dae 19 rzoapsxxo5AQc2NAakt5rfXh8WQ Figure S2E ------- COMMENT: ceb652bddd3380fb 1 KWjBqSQfn0SEPoOyFkfN4SsyxiE (comment: CHECK restored by depletion of ammonium) ------- COMMENT: ceb652bddd3380fb 7 KWjBqSQfn0SEPoOyFkfN4SsyxiE (comment: CHECK restored by depletion of ammonium) ------- COMMENT: ceb652bddd3380fb 8 KWjBqSQfn0SEPoOyFkfN4SsyxiE (comment: CHECK restored by depletion of ammonium) ------- COMMENT: ceb652bddd3380fb 9 KWjBqSQfn0SEPoOyFkfN4SsyxiE (comment: CHECK restored by depletion of ammonium) ------- COMMENT: ceb652bddd3380fb 10 KWjBqSQfn0SEPoOyFkfN4SsyxiE (comment: CHECK restored by depletion of ammonium) ------- COMMENT: ceb652bddd3380fb 14 ip16eL76xdvQEY4T0FmgHDhzPgQ (comment: cell lysis induced by urea) ------- COMMENT: ceb652bddd3380fb 15 ip16eL76xdvQEY4T0FmgHDhzPgQ (comment: cell lysis induced by urea) ------- COMMENT: cecb9f2b2616cb63 2 nZS1y3/XyY6vXS8tGFqYjhuz/m8 As shown in electronic supplementary material, figure S1A, we found that a wee1-50 mad2Δ double mutant is lethal at 36°C. ------- COMMENT: cecb9f2b2616cb63 5 FJWJTo/1LExdDZfWVUXz2WB1Llo As shown in electronic supplementary material, figure S1B, introduction of deletion for mad2+ did not cause the lethality in the cdc2-1w and cdc2-3w mutants. ------- COMMENT: cecb9f2b2616cb63 6 FJWJTo/1LExdDZfWVUXz2WB1Llo As shown in electronic supplementary material, figure S1B, introduction of deletion for mad2+ did not cause the lethality in the cdc2-1w and cdc2-3w mutants. ------- COMMENT: cecb9f2b2616cb63 7 o4iO86eQghBFc2XFHPHh0ybIuVc Time-lapse imaging analysis revealed abnormal positioning of kinetochores in the wee1 (wee1Δ) mutant. ThBecause the kinetochores, which are captured and bioriented, are clus- tered and found on the spindle, the satellite kinetochores remained unattached, or detached from the spindle during the progression to anaphase in the wee1 mutant (electronic supplementary material, figure S2).e cluster of kinetochores frequently fell apart into a main cluster and a small ‘satellite kinetochore’ during mitosis (figure 1b). ------- COMMENT: cecb9f2b2616cb63 8 ArimYRsmgqpPiTR+G9tmIeFJCfI Importantly, the onset of anaphase was postponed until all the satellite kinetochores merged with the main cluster in the mutant (figure 2a,b). ------- COMMENT: cecb9f2b2616cb63 9 ArimYRsmgqpPiTR+G9tmIeFJCfI Importantly, the onset of anaphase was postponed until all the satellite kinetochores merged with the main cluster in the mutant (figure 2a,b). ------- COMMENT: cecb9f2b2616cb63 10 ArimYRsmgqpPiTR+G9tmIeFJCfI Importantly, the onset of anaphase was postponed until all the satellite kinetochores merged with the main cluster in the mutant (figure 2a,b). ------- COMMENT: cecb9f2b2616cb63 11 cWk94hUuQxQ7MYQUt2Fr9INoPZA As mitosis progressed, it was faintly found on the entire length of the spindle and SPB in the wild-type cells (figure 3a). By contrast, Mad2 was found as one or two bright speckles in the vicinity of the spindle during mitosis in the wee1 mutant (figure 3b,c). Judged by the length of the spindle, anaphase was initiated soon after Mad2 speckles disappeared (figure 4a,b). In approximately 28% (28 cells out of 101 cells observed) of the wee1 mutants, the Mad2 speckle appeared at least once (figure 4c). The observation thus suggested that the spindle checkpoint was activated in the wee1 mutants. ------- COMMENT: cecb9f2b2616cb63 12 5THLoPbpdDGaOvsU9MM4FrXKfek s shown in figure 5, the wee1 mutants with no functional spindle check- point indeed failed in accurate chromosome segregation and generated two sister cells with unequal nuclear size. ------- COMMENT: cecb9f2b2616cb63 13 5THLoPbpdDGaOvsU9MM4FrXKfek s shown in figure 5, the wee1 mutants with no functional spindle check- point indeed failed in accurate chromosome segregation and generated two sister cells with unequal nuclear size. ------- COMMENT: cecb9f2b2616cb63 14 5THLoPbpdDGaOvsU9MM4FrXKfek s shown in figure 5, the wee1 mutants with no functional spindle check- point indeed failed in accurate chromosome segregation and generated two sister cells with unequal nuclear size. ------- COMMENT: cecb9f2b2616cb63 15 5THLoPbpdDGaOvsU9MM4FrXKfek s shown in figure 5, the wee1 mutants with no functional spindle check- point indeed failed in accurate chromosome segregation and generated two sister cells with unequal nuclear size. ------- COMMENT: cf0619f59726aa6d 1 DvgOP9kFNEnVJxTyqPG/n1eKV7E (comment: assayed using mammalian proteins) ------- COMMENT: cf28246afde3f15f 1 b0CxYMjfw5JPcJSz2oQZZfndMnA (comment: CHECK mitotic interphase) ------- COMMENT: cf28246afde3f15f 5 f7SHe0Bl3398FHUNPkcyDiojl5g (comment: CHECK exists_during( metaphase? anaphase A????)) ------- COMMENT: cf28246afde3f15f 6 f7SHe0Bl3398FHUNPkcyDiojl5g (comment: CHECK exists_during( metaphase? anaphase A????)) ------- COMMENT: cf2a29ebf81ca19b 13 vqcERX5LVHQWezMyhCP3AnQE92I Fig3C, D ------- COMMENT: cf2a29ebf81ca19b 31 Myh12tndHon8OPLCiHo3dUaWw3s (comment: CHECK https://github.com/pombase/fypo/issues/3931) ------- COMMENT: cf2b7c05db3b7535 6 txQp71m3SZTeIUtltNGXL25LxnY Reduced ssp2-T189 phosphorylation under osmotic stress ------- COMMENT: cf2b7c05db3b7535 7 txQp71m3SZTeIUtltNGXL25LxnY Reduced ssp2-T189 phosphorylation under osmotic stress ------- COMMENT: cf2b7c05db3b7535 8 XTUuj1BIrU7aW8GCpaU4OMWrj+0 Abolished ssp2-T189 phosphorylation under osmotic stress ------- COMMENT: cf2b7c05db3b7535 9 UOVVZ+Di6HLGA6aRC/Q7fLD0gA8 Increased duration of ssp2-T189 phosphorylation under osmotic stress ------- COMMENT: cf2b7c05db3b7535 10 UOVVZ+Di6HLGA6aRC/Q7fLD0gA8 Increased duration of ssp2-T189 phosphorylation under osmotic stress ------- COMMENT: cf2b7c05db3b7535 11 UOVVZ+Di6HLGA6aRC/Q7fLD0gA8 Increased duration of ssp2-T189 phosphorylation under osmotic stress ------- COMMENT: cf2b7c05db3b7535 12 UOVVZ+Di6HLGA6aRC/Q7fLD0gA8 Increased duration of ssp2-T189 phosphorylation under osmotic stress ------- COMMENT: cf3005820af86b6a 1 V72DDwaHmgKfmr79RdFaihx69H4 supp fig 9A ------- COMMENT: cf34c0340e2dff28 12 jVpn1skr4JofPfIlRNtfyQYS1So (comment: Both wtf21 alleles were found at equal frequency in the viable spores.) ------- COMMENT: cf34c0340e2dff28 18 w09spSSBsGySDJfJwt5ddtb6hF0 (comment: S.p. wtf13 assayed; doesn't specify which isoform (or if it's both)) ------- COMMENT: cf34c0340e2dff28 19 UoxtpEa5yFSuJbJjYjj+lY8vumo assayed by expressing S.k. ortholog in S.p.) ------- COMMENT: cf34c0340e2dff28 20 UoxtpEa5yFSuJbJjYjj+lY8vumo assayed by expressing S.k. ortholog in S.p. ------- COMMENT: cf34c0340e2dff28 21 UoxtpEa5yFSuJbJjYjj+lY8vumo assayed by expressing S.k. ortholog in S.p. ------- COMMENT: cf34c0340e2dff28 22 uEDX9XCGf57OU8p+woHV4k7c/Wk inferred from crosses involving hemizygous diploids ------- COMMENT: cf4899040d72592d 4 Xmc0BbE7bV2J9o93gy5A/MCdTwM (comment: happens during metaphase and happens during anaphase. I can't say "decreased during cytokinesis", only option would be to say "not during cytokinesis" which isn't strictly true.) ------- COMMENT: cf4899040d72592d 7 Xmc0BbE7bV2J9o93gy5A/MCdTwM (comment: happens during metaphase and happens during anaphase. I can't say "decreased during cytokinesis", only option would be to say "not during cytokinesis" which isn't strictly true.) ------- COMMENT: cf72468ea0fb98d3 47 tYT3GZPUd7WIV0DqMm/W+nYZ0bY (comment: temperature restrictive for cdc27-P11 alone) ------- COMMENT: cf72468ea0fb98d3 48 tYT3GZPUd7WIV0DqMm/W+nYZ0bY (comment: temperature restrictive for cdc27-P11 alone) ------- COMMENT: cf76a8fb166d50ee 1 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: cf76a8fb166d50ee 4 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: cf76a8fb166d50ee 5 n+90Yw4GWpkoPQ5P6XXMhIWLmgw Since the two fusion proteins, Gar2p-mCherry and WT Pfh1p-GFP, colocalized (Fig. 2B), we conclude that nuclear Pfh1p is found throughout the nucleus but is concentrated in the nucleolus. ------- COMMENT: cf76a8fb166d50ee 6 q2jGDskOzVfyjav9LHkjwHuQqGU WT Pfh1p-GFP colocalized with Rad22p- RFP in distinct nuclear foci (Fig. 2C). ------- COMMENT: cf76a8fb166d50ee 7 y+dZyvMpnPBYn2IGi5Y5ww/NoP8 The pfh1-nuc allele (nuc) did not complement the pfh1D allele, indicating that the mitochondrial isoform of Pfh1p is essential (Table 2, line 4). ------- COMMENT: cf76a8fb166d50ee 8 EIjLrDR2jtcnMKiAG6KA7eUHiSM the pfh1-mt allele (mt) did complement pfh1D (Table 2, line 5) ------- COMMENT: cf76a8fb166d50ee 9 pqIxu6yCJ3f0jcPfSJNpjakZx3k when this strain was grown at 18°C, the pfh1-mt* allele did not complement pfh1D (402/ 407 His+ cells; Table 2, line 7). ------- COMMENT: cf76a8fb166d50ee 10 k8FXEy5dhCm9OW+Y6x1/3ppc0PE Importantly, combination of the pfh1-mt* allele with the pfh1-nuc allele (mt* X nuc) fully complemented the pfh1D strain at 18°C, arguing that the pfh1-mt* strain was indeed deficient in nuclear Pfh1p function (Table 2, line 12). ------- COMMENT: cf76a8fb166d50ee 11 fSSvhcQh6zPg+9r6n0pOleOB8LA because the pfh1-mt* allele did not complement the catalytically inactive pfh1-K338A allele (mt* X cd) (Table 2, line 13), the ATPase/helicase activity of Pfh1p was essential in the nucleus. ------- COMMENT: cf76a8fb166d50ee 12 mbgSkNHY2luMvtY//49tfxXzM9Y but the pfh1-nuc allele in combination with the pfh1-K338A allele (nuc X cd) did not (Table 2, line 16). Therefore, the ATPase/helicase activity of Pfh1p was also essential in mitochondria. ------- COMMENT: cf76a8fb166d50ee 13 UtzcOnJvAqta+q5EvLA4pCWAANI Based on their elongated cellular morphology, and large number of Rad22 foci, we conclude that cells depleted of nuclear Pfh1p suffer persistent DNA damage, and this damage triggers a G2 -phase arrest. ------- COMMENT: cf76a8fb166d50ee 14 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: cf76a8fb166d50ee 15 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: cf76a8fb166d50ee 16 Mfg961uvqsP3Bwc59eYC7fYZXc0 Pfh1p was also essential in mitochondria (Table 2), where its depletion resulted in rapid loss of mtDNA and very slow growth (Fig. 3 and 5). ------- COMMENT: cfa0410c2c949ac3 122 IP4i7+pqkl0K+lJqcpCUKeTc89c present throughout mitotic cell cycle ------- COMMENT: cfa0410c2c949ac3 130 8Cjoc37lKiuKs3UoABPfr/cCZmI (comment: CHECK slighly more severe than sir2+ overexpression alone) ------- COMMENT: cfa0410c2c949ac3 131 /ehAOYp/amXb67zn2WQqBmvb4wE (comment: CHECK same as sir2+ overexpression alone) ------- COMMENT: cfa1ac27dc7acf6c 2 xoZXsC6UhEmwiVlSgTgbMPvkyro (comment: at 33.5 degrees, which is restrictive for cdc2-33 but allows sporulation) ------- COMMENT: cfa1ac27dc7acf6c 4 7MptJRghkt4AApItLVRp7NLcrSI (comment: at 33.5 degrees, which is restrictive for cdc10129 but allows sporulation) ------- COMMENT: cfa1ac27dc7acf6c 6 a3qUYGhfvtSEMaCD1Zb21N6VDXw (comment: done in h- cells kinetics depend on medium composition (see fig 6B)) ------- COMMENT: cfa1ac27dc7acf6c 25 xoZXsC6UhEmwiVlSgTgbMPvkyro (comment: at 33.5 degrees, which is restrictive for cdc2-33 but allows sporulation) ------- COMMENT: cfa1ac27dc7acf6c 29 lcXmppDxFY/xzlh2lJRQJmGG20k (comment: at 33.5 degrees, which is restrictive for cdc10-129 but allows sporulation) ------- COMMENT: cfba7cb5d851df37 1 xvPCvQHkQpNhpzkkiEhZa0jyNeY xap5 genetically interacts with pht1 to repress antisense transcripts. In the ∆xap5∆pht1 double mutants the level of antisense transcription is exacerbated as observed using RNA-seq. Selected loci also showed antisense RNA production in histone deacetylase (HDACs) gene mutants. ------- COMMENT: cfba7cb5d851df37 77 6DHVFES2erODlBGKTyaZQbzfDmg (comment: CHECK SO:0000141 = The sequence of DNA located either at the end of the transcript that causes RNA polymerase to terminate transcription. SO:0000186 - LTR SO:0000101 - transposable element) ------- COMMENT: cfc48f029a6c9660 1 NwAiV2Wb4wTHNJoWw6fplmkRQGo Pef1 ablation or chemical inactivation of its kinase activity stimulates Rad21 and Mis4 binding to their cognates sites on chromosomes. The effect in most prominent in the G1 phase of the mitotic cycle. ------- COMMENT: cfc48f029a6c9660 2 aJqjckTbZZGGfaEYpHRt4t8YwkE Psm1 and Mis4 are found in Pef1 immunoprecipitates (Fig. 5AB) ------- COMMENT: cfc48f029a6c9660 3 nioVF4ftFxVOLXh7ck8K3OFZWqg Fig. 7. The phenotype is exacerbated by pph3 deletion and rescued by pef1 deletion ------- COMMENT: cfc48f029a6c9660 4 gVGqZ0mI08dAK/4lEzlbPbUDtlM Figure 2—figure supplement 1 ------- COMMENT: cfc48f029a6c9660 5 nubi9kk+OP5U1DidW0y8lZgZS1Y (comment: CONDITION 36.5°C) ------- COMMENT: cfc48f029a6c9660 6 Of4Bc3ljQmrBP9IvQ8cc3/G43Fk Fig.1 (comment: CONDITION 36.5°C) ------- COMMENT: cfc48f029a6c9660 7 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: cfc48f029a6c9660 8 jFebtirvxFFc71lqoXAhEfKD7TE Fig. 1 (comment: CONDITION 36.5°C) ------- COMMENT: cfc48f029a6c9660 9 02l+3cqTh7UTFJSYjyKhzJabH68 fig1c ------- COMMENT: cfc48f029a6c9660 12 VaXiSZZO7R52KMDhEepRtSmtYgA Fig.1 ------- COMMENT: cfc48f029a6c9660 13 VaXiSZZO7R52KMDhEepRtSmtYgA Fig.1 ------- COMMENT: cfc48f029a6c9660 14 g8uTLsZDkrIjSxhImLQuKYO5mwc Figure 1—figure supplement 1 ------- COMMENT: cfc48f029a6c9660 15 g8uTLsZDkrIjSxhImLQuKYO5mwc Figure 1—figure supplement 1 ------- COMMENT: cfc48f029a6c9660 16 2zKgOwlk17NcJ6CZmHX4unY8CB8 Figure 1—figure supplement 1. "although colonies were tiny and grew very slowly" ------- COMMENT: cfc48f029a6c9660 17 KDhxFzxSAty66l2akAJRiLOcPEM Fig.5 ------- COMMENT: cfc48f029a6c9660 18 GVBVibsPoz9vNHXFS686zKJO+24 Phosphorylates Rad21 on threonine 262. Fig.5 ------- COMMENT: cfc48f029a6c9660 19 W7TApI3dyxbrPGjcSGcxCOWoe2k Fig.7 ------- COMMENT: cfc48f029a6c9660 23 W7TApI3dyxbrPGjcSGcxCOWoe2k Fig.7 ------- COMMENT: cfc48f029a6c9660 24 ud4UFC/uFxWHk1t2W+DeHW7sHC8 Fig.7 (comment: CONDITION 34°C) ------- COMMENT: cfc48f029a6c9660 25 W7TApI3dyxbrPGjcSGcxCOWoe2k Fig.7 ------- COMMENT: cfc48f029a6c9660 26 0P6Z0JbREDJkhZQ6RJu3WfH9Bso Fig.7. Phenotype suppressed by the deletion of pef1 ------- COMMENT: cfc48f029a6c9660 27 Pn4ycTQ9jqHVvNJl4eliLFbKWKg (comment: CONDITION 34°C), Fig.7 ------- COMMENT: cfc48f029a6c9660 28 Pn4ycTQ9jqHVvNJl4eliLFbKWKg (comment: CONDITION 34°C), Fig.7 ------- COMMENT: cfc48f029a6c9660 29 KDhxFzxSAty66l2akAJRiLOcPEM Fig.5 ------- COMMENT: cfc48f029a6c9660 30 4sq3LSeKPdXGBEH9NIfv7ZdZqII Fig.8 ------- COMMENT: cfc48f029a6c9660 31 4sq3LSeKPdXGBEH9NIfv7ZdZqII Fig.8 ------- COMMENT: cfc48f029a6c9660 32 4sq3LSeKPdXGBEH9NIfv7ZdZqII Fig.8 ------- COMMENT: cfc48f029a6c9660 33 4sq3LSeKPdXGBEH9NIfv7ZdZqII Fig.8 ------- COMMENT: cfc48f029a6c9660 34 4sq3LSeKPdXGBEH9NIfv7ZdZqII Fig.8 ------- COMMENT: cfc48f029a6c9660 35 4sq3LSeKPdXGBEH9NIfv7ZdZqII Fig.8 ------- COMMENT: cfc48f029a6c9660 36 4sq3LSeKPdXGBEH9NIfv7ZdZqII Fig.8 ------- COMMENT: cfc48f029a6c9660 37 MsNq6r0PNjkd5LSImvSt2YgOkck (comment: CHECK mis4-367(aaG1487D) pef1Δ) ------- COMMENT: cfc48f029a6c9660 44 R7HaNIP4dcSWXs1QnFMzF+wJ4b8 (comment: CHECK mis4-367(aaG1487D) pas1Δ) ------- COMMENT: cfc48f029a6c9660 45 JVcmPkvGYxpSHvOXPEBueZLcvH0 (comment: CHECK mis4-367(aaG1487D) clg1Δ) ------- COMMENT: cfc48f029a6c9660 46 0jInezJa8OXRoSJFEmhUIy3UlFQ (comment: CHECK mis4-367(aaG1487D) psl1Δ) ------- COMMENT: cfc48f029a6c9660 47 TZq2k/8CZniCbWwnOFLSl7yvO2w (comment: CHECK mis4-367(aaG1487D) rad21-T262A) ------- COMMENT: cfc48f029a6c9660 48 xGeKBmELXVcdn8IxGUwnUIaTYAE (comment: CHECK mis4-367(aaG1487D) rad21-T262E) ------- COMMENT: cfc48f029a6c9660 49 xVmuM7whn94UaIXrXzCawQEjKAw (comment: CHECK mis4-367(aaG1487D) ppe2Δ) ------- COMMENT: cfc48f029a6c9660 50 mxJB4MVrFvQ72QcF4ZEGhvbL/Mc (comment: CHECK eso1-H17(aaG799D) pef1Δ) ------- COMMENT: cfc48f029a6c9660 51 PXArobeV1vxu/oqlYeEECvwNxa0 (comment: CHECK psm3-K105N K106N ppe2Δ) ------- COMMENT: cfc48f029a6c9660 52 VaXiSZZO7R52KMDhEepRtSmtYgA Fig.1 ------- COMMENT: cfc48f029a6c9660 56 SQgaH4n7CY+a/asdDfwvJLy6DRA Pef1 phosphorylates Rad21 in vitro ------- COMMENT: cfc48f029a6c9660 57 hXvZ3YOWPYmDLSfYblTD39G7H9E (comment: in vitro phosphorylation assay): Rad21-T262 phosphorylation by Pef1 is dependent on Psl1 ------- COMMENT: cfc48f029a6c9660 58 VaXiSZZO7R52KMDhEepRtSmtYgA Fig.1 ------- COMMENT: cfc48f029a6c9660 59 VaXiSZZO7R52KMDhEepRtSmtYgA Fig.1 ------- COMMENT: cfc48f029a6c9660 60 uVj2lqUZPErc+fxew/ap6rsaIzI Fig.6 ------- COMMENT: cfc48f029a6c9660 63 W7TApI3dyxbrPGjcSGcxCOWoe2k Fig.7 ------- COMMENT: cfc48f029a6c9660 64 uHcbP3A12RZCj3DA224zQkcAEfg Fig.4 ------- COMMENT: cfc48f029a6c9660 66 pOoq2mqyn55xFOqMk517sRxH2KY (comment: CHECK mis4-242 (G1326E aa) pef1Δ) ------- COMMENT: cfc48f029a6c9660 67 VDczTT/iS8MoqNE4gOMH/CLvcvU Fig.7. (comment: CHECK Phenotype suppressed by the deletion of the pef1 gene) ------- COMMENT: cfc48f029a6c9660 68 UE9VFTCoK+6avAkaCUPxCI0PHJE Fig.1b ------- COMMENT: cfc48f029a6c9660 69 GWSjC/oD53fs7If59RJTllkN4iQ Fig.2 supp1 ------- COMMENT: cfc48f029a6c9660 70 ar9e1aKJM0P3CHij6Xn+AMDRz94 Figure 5Dm : "In vitro Rad21 phosphorylation was abolished when Pef1 was purified from psl1 deleted cells" ------- COMMENT: cfc48f029a6c9660 71 Ox0v0gcN5se+J2WsHFoSThTpqa8 (Figure 5E, Figure 5—figure supplement 1). Replacement of T262 by an alanine abolished in vitro Rad21 phosphorylation by Pef1-GFP ------- COMMENT: cfc48f029a6c9660 73 R9OpQUO5wrwh0Tsm/4mco9I4RdY fig 5g ------- COMMENT: cfc48f029a6c9660 77 tMZMtWzkrP8fSd2/IN95M7Urh+w (comment: antagonises pef1) ------- COMMENT: cfe162f89d8c435c 31 2PgFnL2DO9EPyL5UIxRKNJHmtNM (comment: maybe not shown strongly in this paper but I'm trying to get the git genes annotated to this term because pka1 phosphorylates rst2 which excludes rst2 from the nucleus. rst2 when in the nucleus activates ste11 transcription.) ------- COMMENT: d00159ad077f1b3c 14 szKLJ1OlkHg6fWHSFxXib0hqd7g (comment: no MF possible) ------- COMMENT: d00159ad077f1b3c 69 3Ok9at/BdoRW1BEkNn8RcLM6DM0 (comment: it affects suc22 binding to cdc22. There is no evidence thta it is involved in catabolism and I dont think I can make a MF from it.) ------- COMMENT: d00aadd46396cb11 1 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: d00aadd46396cb11 2 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: d00aadd46396cb11 3 uL0IPdicnMe3HTwpu/MdIRk78EI Supplemen- tary Figure S3A ------- COMMENT: d00aadd46396cb11 4 jJGArrew4px1dGhouXfByt7M2L4 Supplemen- tary Figure S3A NaCl or KCl) ------- COMMENT: d00aadd46396cb11 5 YF0aB6QVCeKm5Q1FHPAXLuoOgxU Supplementary Figure S1B ------- COMMENT: d00aadd46396cb11 6 YF0aB6QVCeKm5Q1FHPAXLuoOgxU Supplementary Figure S1B ------- COMMENT: d00aadd46396cb11 7 ksu954H+qIPtA04cG1wZtxSXom4 subcategory of oxidative stress known as GSH or disulfide stress (4). Indeed, a lower GSH/GSSG ratio was found after treatment with diamide or Cd (Supplementary Figure S4) in WT and in the SPBC29A10.12Δ strain ------- COMMENT: d00aadd46396cb11 8 1VBkQeaYOm4enagMUWQJRUYBgaU (comment: CHECK detoxification of thiol disulphide (in response to disulphide stress)) ------- COMMENT: d00aadd46396cb11 18 1VBkQeaYOm4enagMUWQJRUYBgaU (comment: CHECK detoxification of thiol disulphide (in response to disulphide stress)) ------- COMMENT: d00aadd46396cb11 20 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: d00aadd46396cb11 22 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: d00aadd46396cb11 23 LH3s0bT+0hTdxo9FWJS6A8GiaxY (comment: diamide-induced promoters) ------- COMMENT: d00aadd46396cb11 24 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: d00aadd46396cb11 25 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: d00aadd46396cb11 31 1VBkQeaYOm4enagMUWQJRUYBgaU (comment: CHECK detoxification of thiol disulphide (in response to disulphide stress)) ------- COMMENT: d05448f3a0821419 1 N6jC+U3A2RRl/gZXzHyuvIb3pIk (comment: CHECK throughout_cell_cycle) ------- COMMENT: d0c2d05f4db2d947 1 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: d0c2d05f4db2d947 2 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: d0c2d05f4db2d947 3 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: d0c2d05f4db2d947 4 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: d0c2d05f4db2d947 5 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: d0dd7cb7b7db147e 6 hvHRWX6y/yrVikIuKsO1BmNLJCg (comment: CHECK S326, T429, S499: added by cyclin-dependent kinase (Cdk1)) ------- COMMENT: d0dd7cb7b7db147e 7 EGn39IM0bPGtT28Co0pRR6y4TBQ (comment: CHECK S248, T412, T502, S533: added by cyclin-dependent kinase (Cdk1)) ------- COMMENT: d0dd7cb7b7db147e 12 JteODbkajWu3n2DBfzqA0D6zsWQ (comment: CHECK Term name: supports establishment of SIN asymmetry Definition: characterized by asymmetric localization of the SIN initiator kinase Cdc7 in anaphase) ------- COMMENT: d0dd7cb7b7db147e 15 hROQM7fCDA/9FliyGhIhCgKvmb4 (comment: CHECK affecting Cdc7) ------- COMMENT: d0dd7cb7b7db147e 16 xYlrjp+hJ7+Cjd7EZwbonzB2QmA (comment: CHECK targets Byr4 at S248A, S326A, T412A, T429A, S499A, T502A, S533A, results in Byr4 removal from metaphase spindle pole bodies) ------- COMMENT: d0dd7cb7b7db147e 22 Wux/hBw6257W31P5vDsLfyjUu5M (comment: CHECK Cdk1-dependent, Cdk1 non-phosphorylatable Byr4 localizes to one or both SPBs in >90% of metaphase cells) ------- COMMENT: d0dd7cb7b7db147e 28 3R5wL0RvnSF3VYswLOUj4LV+zXw (comment: CHECK low penetrance) ------- COMMENT: d0dd7cb7b7db147e 37 kKXWdM1tBFiwGOJbgbnrPSA9lnQ results from collapse of actomyosin contractile ring ------- COMMENT: d0dd7cb7b7db147e 45 4c/fDrFVT8LBj/BN1qyUkfo9vO4 (comment: CHECK medium penetrance) ------- COMMENT: d0dd7cb7b7db147e 50 0i7RhI8bJXcBhlwb8cK8j8nCvO4 (comment: CHECK anaphase B) ------- COMMENT: d1339185f567b117 1 lf21VrAGM8QeNF2UeRMQ8ioEsJw By contrast, Fkbp41 is found pri- marily in the insoluble chromatin fraction (Figure S1C). ------- COMMENT: d1339185f567b117 2 BO/B8AZ876g2YJDIOk3QHokwrvY We found that chromatin-associated Fkbp39 and Fkbp41 predominately localized to the rDNA locus, similar to previous observations on budding yeast and mammalian nucle- ophosmins,20–23 but our ChIP-seq data also revealed a strong enrichment at the 25S rDNA, which had not been described before (Figures 1A, 1B, and S1D). ------- COMMENT: d1339185f567b117 3 BO/B8AZ876g2YJDIOk3QHokwrvY We found that chromatin-associated Fkbp39 and Fkbp41 predominately localized to the rDNA locus, similar to previous observations on budding yeast and mammalian nucle- ophosmins,20–23 but our ChIP-seq data also revealed a strong enrichment at the 25S rDNA, which had not been described before (Figures 1A, 1B, and S1D). ------- COMMENT: d1339185f567b117 4 Pry2lIqLjhbzUjHqUS+4aWqKYxA On the other hand, Fkbp39 could bind to DNA and nucleosomes with a 40-bp-long linker DNA (Figures 1C, S1I, and S1J) but not to nucleosomes lacking or with short 10 bp linker DNA (Figure S2A). ------- COMMENT: d1339185f567b117 5 Pry2lIqLjhbzUjHqUS+4aWqKYxA On the other hand, Fkbp39 could bind to DNA and nucleosomes with a 40-bp-long linker DNA (Figures 1C, S1I, and S1J) but not to nucleosomes lacking or with short 10 bp linker DNA (Figure S2A). ------- COMMENT: d1339185f567b117 6 HWAnys41Uq4HdYEi3IGqWaX3vrU We mutated the active site of the prolyl isomerase domain of Fkbp39 (F301C/W314C/Y337K)29 and found that it was impaired in the ability to bind nucleosomes but not DNA (Figure S2B). ------- COMMENT: d1339185f567b117 7 0boKz2ln6+m5Sqd0F7F4Tue2SgI This supports the role of histones in promoting heterotypic LLPS of Fkbp39 with DNA (Figures 1D and S2E). These observations suggest that Fkbp39 could organize the rDNA-containing chromatin into a phase- separated compartment. ------- COMMENT: d1339185f567b117 21 cmpUVIy1As1x71ftD6xU07fr5Qk We identi- fied 4 major states of nascent 60S, and each state could be further classified into multiple substates (Figures 2A and S3; Table 1). The nascent 60S associated with Fkbp39 represents early ribosome biogenesis stages as described below. ------- COMMENT: d1339185f567b117 22 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 23 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 24 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 25 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 26 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 27 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 28 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 29 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 30 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 31 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 32 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 33 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 34 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 35 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 36 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 37 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 38 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 39 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 40 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 41 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 42 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 43 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 44 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 45 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 46 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 47 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 48 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 49 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 50 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 51 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 52 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 53 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 54 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 55 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 56 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 57 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 58 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 59 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 60 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 61 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 62 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 63 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 64 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 65 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 66 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 67 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 68 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 69 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 70 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 71 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 72 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 73 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 74 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 75 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 76 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 77 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 78 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 79 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 80 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 81 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 82 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 83 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 84 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 85 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 86 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 87 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 88 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 89 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 90 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d1339185f567b117 91 3oB6eED6U+lA0IAC5uPbzbd8eLA In state 3, RNA domain III is folded and the biogenesis complex Ytm1-Erb1-Ppp1 (Ytm1-Erb1-Nop7 in S. cerevisiae) is incorporated32 for a total of 30 ribosomal proteins and 15 biogenesis factors (Figure 2A). ------- COMMENT: d1339185f567b117 92 3oB6eED6U+lA0IAC5uPbzbd8eLA In state 3, RNA domain III is folded and the biogenesis complex Ytm1-Erb1-Ppp1 (Ytm1-Erb1-Nop7 in S. cerevisiae) is incorporated32 for a total of 30 ribosomal proteins and 15 biogenesis factors (Figure 2A). ------- COMMENT: d1339185f567b117 93 3oB6eED6U+lA0IAC5uPbzbd8eLA In state 3, RNA domain III is folded and the biogenesis complex Ytm1-Erb1-Ppp1 (Ytm1-Erb1-Nop7 in S. cerevisiae) is incorporated32 for a total of 30 ribosomal proteins and 15 biogenesis factors (Figure 2A). ------- COMMENT: d1339185f567b117 94 JYlUKfxxVeOhENdooN/PsQxx5+g State 2 has 24 ribosomal proteins but only 9 biogenesis factors, and ITS2 is cleaved and its associated factors are released (Figures 2A and S3D; Table S2). ------- COMMENT: d1339185f567b117 95 JYlUKfxxVeOhENdooN/PsQxx5+g State 2 has 24 ribosomal proteins but only 9 biogenesis factors, and ITS2 is cleaved and its associated factors are released (Figures 2A and S3D; Table S2). ------- COMMENT: d1339185f567b117 96 JYlUKfxxVeOhENdooN/PsQxx5+g State 2 has 24 ribosomal proteins but only 9 biogenesis factors, and ITS2 is cleaved and its associated factors are released (Figures 2A and S3D; Table S2). ------- COMMENT: d1339185f567b117 97 JYlUKfxxVeOhENdooN/PsQxx5+g State 2 has 24 ribosomal proteins but only 9 biogenesis factors, and ITS2 is cleaved and its associated factors are released (Figures 2A and S3D; Table S2). ------- COMMENT: d1339185f567b117 111 CexiOfNMP2oN936dgpZc9zH+jtc Similarly to human nucleosphosmin,12 Fkbp39 can bind RNA in vitro and phase separate with RNA (Figures S4A and S4B). Fkbp39 robustly forms liquid-like condensates with RNA in sharp contrast to condensates with DNA (Figures S4B and S2D). ------- COMMENT: d1339185f567b117 112 iAwFjkJw9cC5t/+HxEaSuAAPKD4 We isolated nascent 40S (also known as small subunit processome) by pull- ing down fibrillarin (Nop1)-associated particles (Figure S4I; Table S3; Data S1) ------- COMMENT: d1339185f567b117 113 iAwFjkJw9cC5t/+HxEaSuAAPKD4 We isolated nascent 40S (also known as small subunit processome) by pull- ing down fibrillarin (Nop1)-associated particles (Figure S4I; Table S3; Data S1) ------- COMMENT: d1339185f567b117 114 iAwFjkJw9cC5t/+HxEaSuAAPKD4 We isolated nascent 40S (also known as small subunit processome) by pull- ing down fibrillarin (Nop1)-associated particles (Figure S4I; Table S3; Data S1) ------- COMMENT: d1339185f567b117 115 iAwFjkJw9cC5t/+HxEaSuAAPKD4 We isolated nascent 40S (also known as small subunit processome) by pull- ing down fibrillarin (Nop1)-associated particles (Figure S4I; Table S3; Data S1) ------- COMMENT: d1339185f567b117 116 iAwFjkJw9cC5t/+HxEaSuAAPKD4 We isolated nascent 40S (also known as small subunit processome) by pull- ing down fibrillarin (Nop1)-associated particles (Figure S4I; Table S3; Data S1) ------- COMMENT: d1339185f567b117 117 iAwFjkJw9cC5t/+HxEaSuAAPKD4 We isolated nascent 40S (also known as small subunit processome) by pull- ing down fibrillarin (Nop1)-associated particles (Figure S4I; Table S3; Data S1) ------- COMMENT: d1339185f567b117 118 iAwFjkJw9cC5t/+HxEaSuAAPKD4 We isolated nascent 40S (also known as small subunit processome) by pull- ing down fibrillarin (Nop1)-associated particles (Figure S4I; Table S3; Data S1) ------- COMMENT: d1339185f567b117 119 iAwFjkJw9cC5t/+HxEaSuAAPKD4 We isolated nascent 40S (also known as small subunit processome) by pull- ing down fibrillarin (Nop1)-associated particles (Figure S4I; Table S3; Data S1) ------- COMMENT: d1339185f567b117 120 iAwFjkJw9cC5t/+HxEaSuAAPKD4 We isolated nascent 40S (also known as small subunit processome) by pull- ing down fibrillarin (Nop1)-associated particles (Figure S4I; Table S3; Data S1) ------- COMMENT: d1339185f567b117 121 iAwFjkJw9cC5t/+HxEaSuAAPKD4 We isolated nascent 40S (also known as small subunit processome) by pull- ing down fibrillarin (Nop1)-associated particles (Figure S4I; Table S3; Data S1) ------- COMMENT: d1339185f567b117 122 iAwFjkJw9cC5t/+HxEaSuAAPKD4 We isolated nascent 40S (also known as small subunit processome) by pull- ing down fibrillarin (Nop1)-associated particles (Figure S4I; Table S3; Data S1) ------- COMMENT: d1339185f567b117 123 iAwFjkJw9cC5t/+HxEaSuAAPKD4 We isolated nascent 40S (also known as small subunit processome) by pull- ing down fibrillarin (Nop1)-associated particles (Figure S4I; Table S3; Data S1) ------- COMMENT: d1339185f567b117 124 iAwFjkJw9cC5t/+HxEaSuAAPKD4 We isolated nascent 40S (also known as small subunit processome) by pull- ing down fibrillarin (Nop1)-associated particles (Figure S4I; Table S3; Data S1) ------- COMMENT: d1339185f567b117 125 iAwFjkJw9cC5t/+HxEaSuAAPKD4 We isolated nascent 40S (also known as small subunit processome) by pull- ing down fibrillarin (Nop1)-associated particles (Figure S4I; Table S3; Data S1) ------- COMMENT: d1339185f567b117 126 hfUe9rPOmj32D4H1r1NRp5juCEI In wild-type cells, Ytm1 is not detectable on chromatin, in agreement with our in vitro data showing that Fkbp39 sepa- rates ribosome biogenesis intermediates from chromatin (Figures 4A, S5A, and S5B). ------- COMMENT: d1339185f567b117 127 FwAW+uBN7lmxfjalpfD6sGWhSwY By contrast, in fkbp39D cells, we de- tected Ytm1 on chromatin, primarily at the 30 end of the rDNA re- peats, specifically enriched at the 30 ETS and the non-tran- scribed spacer (NTS) DNA sites (Figures 4A, S5A, and S5B). ------- COMMENT: d1339185f567b117 128 Kk2pEJy9aDZA813BBYBK6FIMgCw Fkbp39 separates nascent ribosomes from chromatin in cells ------- COMMENT: d1339185f567b117 129 Kk2pEJy9aDZA813BBYBK6FIMgCw Fkbp39 separates nascent ribosomes from chromatin in cells ------- COMMENT: d1339185f567b117 130 gjJo1ecnWWmKl41wHcEsM6vqET4 reduced levels of 18S and 25S rRNA and accu- mulation of 50 ETS RNA and unprocessed rRNA in the mutant compared with wild-type cells (Figures S5D and S5E). ------- COMMENT: d1339185f567b117 131 gjJo1ecnWWmKl41wHcEsM6vqET4 reduced levels of 18S and 25S rRNA and accu- mulation of 50 ETS RNA and unprocessed rRNA in the mutant compared with wild-type cells (Figures S5D and S5E). ------- COMMENT: d1339185f567b117 132 nxzVxqPhN84lFqBsiKFQTEk7HMw To determine if Fkbp39 is required for transcription or RNA processing, we performed nascent RNA-seq using 4-thiouracil labeling. Immediately after labeling, the levels of nascent rRNA in wild-type or fkbp39D cells were comparable (Figures 4B, S5F, and S5G), indicating that Fkbp39 is not required for rRNA transcription. ------- COMMENT: d1339185f567b117 135 SXhFra7iw8xgp63nF4VRQ2agHoo Although the overall Fkbp39 protein levels are slightly reduced in fkbp41D total lysates (Fig- ure S7E), there was a marked reduction of Fkbp39 localization specifically at 25S rDNA in those cells compared with wild- type (Figures 6A, 6B, and S7F). ------- COMMENT: d1339185f567b117 136 03cLhDTYDCvehdwm4XAHFjg3vn0 Fkbp41 also in- teracts with components from the transcription machinery, spe- cifically Leo1, a subunit from the Paf1 complex, which promotes RNA Polymerase I transcription elongation (Figures 6E and S7I).37 No interactions between Fkbp39 and transcription elon- gation factors were detected by mass spectrometry (Table S5). Overall, our data suggest that Fkbp41 is recruited to the 25S rDNA site by interaction with the transcription machinery, where it promotes specific Fkbp39 localization. ------- COMMENT: d1339185f567b117 138 Q4/XLEClFNIoaCt7D9cFea/5/tE The structure of state 1 shows an early assembly intermediate with 23 ribosomal proteins and 19 biogenesis factors (Figure 2A; Table S2). ------- COMMENT: d150d3eeabbfefdd 4 vzMVXrKgXBBNHB3ToYqYZWtvefQ fig 1 - (comment: They say they use YES in methods and fig2) ------- COMMENT: d150d3eeabbfefdd 5 vzMVXrKgXBBNHB3ToYqYZWtvefQ fig 1 - (comment: They say they use YES in methods and fig2) ------- COMMENT: d150d3eeabbfefdd 6 vzMVXrKgXBBNHB3ToYqYZWtvefQ fig 1 - (comment: They say they use YES in methods and fig2) ------- COMMENT: d150d3eeabbfefdd 7 vzMVXrKgXBBNHB3ToYqYZWtvefQ fig 1 - (comment: They say they use YES in methods and fig2) ------- COMMENT: d150d3eeabbfefdd 8 vzMVXrKgXBBNHB3ToYqYZWtvefQ fig 1 - (comment: They say they use YES in methods and fig2) ------- COMMENT: d150d3eeabbfefdd 9 vzMVXrKgXBBNHB3ToYqYZWtvefQ fig 1 - (comment: They say they use YES in methods and fig2) ------- COMMENT: d150d3eeabbfefdd 10 vzMVXrKgXBBNHB3ToYqYZWtvefQ fig 1 - (comment: They say they use YES in methods and fig2) ------- COMMENT: d150d3eeabbfefdd 11 vzMVXrKgXBBNHB3ToYqYZWtvefQ fig 1 - (comment: They say they use YES in methods and fig2) ------- COMMENT: d150d3eeabbfefdd 12 XPnM32shLX68Eo2rR66Yrm1uKgQ fig 1c ------- COMMENT: d150d3eeabbfefdd 13 lgEOXxYwArQhMMYeB//KkZLCMV8 (comment: I can never remember why e.g. sodium chloride isn't a child to salt stress) ------- COMMENT: d150d3eeabbfefdd 14 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: d150d3eeabbfefdd 15 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: d150d3eeabbfefdd 16 3IIZKd5m6W0pGR2ur3U7rMT0A9A fig 1d ------- COMMENT: d150d3eeabbfefdd 17 3IIZKd5m6W0pGR2ur3U7rMT0A9A fig 1d ------- COMMENT: d150d3eeabbfefdd 18 3IIZKd5m6W0pGR2ur3U7rMT0A9A fig 1d ------- COMMENT: d150d3eeabbfefdd 19 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: d150d3eeabbfefdd 20 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: d150d3eeabbfefdd 21 CpLPgm8i0a3op3IoshdhYxoBsbU fig 2c ------- COMMENT: d150d3eeabbfefdd 22 CpLPgm8i0a3op3IoshdhYxoBsbU fig 2c ------- COMMENT: d150d3eeabbfefdd 23 ssUPqcbJYiCo3qgvOoizS47vzUQ fig 2d ------- COMMENT: d150d3eeabbfefdd 24 ssUPqcbJYiCo3qgvOoizS47vzUQ fig 2d ------- COMMENT: d150d3eeabbfefdd 25 iXHIAi0t+dy4Wg/hrg7PPdT1qxY fig 2e ------- COMMENT: d150d3eeabbfefdd 26 iXHIAi0t+dy4Wg/hrg7PPdT1qxY fig 2e ------- COMMENT: d150d3eeabbfefdd 27 W4egHp8nZtd5iNYpwU05IAEyjwY fig 3d ------- COMMENT: d150d3eeabbfefdd 28 W4egHp8nZtd5iNYpwU05IAEyjwY fig 3d ------- COMMENT: d150d3eeabbfefdd 29 vzMVXrKgXBBNHB3ToYqYZWtvefQ fig 1 - (comment: They say they use YES in methods and fig2) ------- COMMENT: d150d3eeabbfefdd 30 vzMVXrKgXBBNHB3ToYqYZWtvefQ fig 1 - (comment: They say they use YES in methods and fig2) ------- COMMENT: d150d3eeabbfefdd 31 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: d150d3eeabbfefdd 32 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: d150d3eeabbfefdd 33 23A7gKYH++mwxpDaLPxFOcAYx6Y fig 3c ------- COMMENT: d150d3eeabbfefdd 34 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: d150d3eeabbfefdd 35 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: d150d3eeabbfefdd 36 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: d150d3eeabbfefdd 37 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: d150d3eeabbfefdd 38 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: d150d3eeabbfefdd 39 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: d150d3eeabbfefdd 40 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: d150d3eeabbfefdd 41 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: d150d3eeabbfefdd 42 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: d150d3eeabbfefdd 43 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: d150d3eeabbfefdd 44 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: d150d3eeabbfefdd 47 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: d150d3eeabbfefdd 48 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: d150d3eeabbfefdd 49 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: d150d3eeabbfefdd 50 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: d150d3eeabbfefdd 51 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: d150d3eeabbfefdd 52 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: d150d3eeabbfefdd 53 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: d150d3eeabbfefdd 54 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: d150d3eeabbfefdd 55 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: d150d3eeabbfefdd 56 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: d150d3eeabbfefdd 57 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: d150d3eeabbfefdd 58 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: d150d3eeabbfefdd 59 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: d150d3eeabbfefdd 60 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: d150d3eeabbfefdd 61 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: d150d3eeabbfefdd 62 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: d150d3eeabbfefdd 63 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: d1ca5d873c81c5fa 3 Ep8zZzN//VHzkjusvf1UMdbMjBc An fragile actomyosin contractile ring is an abnormal actomyosin contractile ring that disassemble by treatment with a low dose of Latrunculin A, an actin depolymerizing agent. ------- COMMENT: d1ca5d873c81c5fa 4 mKw2alEwGQNN4zv2JMlOY5Yy1EM (comment: CHECK *****(abnormal distribution in...)) inhomogeneous contractile Rng2 ring An inhomogeneous contractile Rng2 ring is an abnormal actomyosin contractile ring that show an uneven distribution of 2mYFP-Rng2-12A over the ring. ------- COMMENT: d1ca5d873c81c5fa 8 Ep8zZzN//VHzkjusvf1UMdbMjBc An fragile actomyosin contractile ring is an abnormal actomyosin contractile ring that disassemble by treatment with a low dose of Latrunculin A, an actin depolymerizing agent. ------- COMMENT: d1e54bf1d660f4c8 1 TQVJlEZozWCcXVpa3KfM8kDqbok (comment: binds to 54-bp element at 1186-1239) ------- COMMENT: d1eebaf912739707 2 x4ip2frI1aOWFQ/6IcGWpQtXsJ4 (comment: Cdr2 does not exit nodes (unlike Cdr1) upon osmotic stress) ------- COMMENT: d1eebaf912739707 5 mgjFPBTpBDexuWbLqOwtzQoYEdY combination of in vitro kinase assay and mutant phenotypes ------- COMMENT: d1eebaf912739707 23 J+zvqHeb/Jg78GrulFwt50rfzH8 Cdr1-K41A remains unphosphorylated; Cdr1+ not tagged ------- COMMENT: d1eebaf912739707 32 Xq5Ip/7VG5GKAc6wi8lQ8Vhe9o8 Cdr1-K41A remains in nodes; Cdr1+ not tagged ------- COMMENT: d21245ab5467d47b 2 lIxrXlifyMhHa5RD1I9cbg2p0kA fig 3 At exg1, ecm33, eng1 et gas1, condensin is redistributed throughout the gene body instead of accumulating around transcription termination sites. ------- COMMENT: d21245ab5467d47b 3 29pYgxp1oeb8CUooiyPzWN1YcE0 fig 2b Importantly, the accumulation of condensin in sen1Δ cells could not be caused by an accumulation of either TFIIIC or Tbp1 because their levels on chromatin remained largely unaffected in the absence of Sen1, as shown by ChIP with a GFP-tagged version of Tbp1 and a myc-tagged version of the TFIIIC component Sfc6 (Figs 2B and S1). ------- COMMENT: d21245ab5467d47b 4 Fau9ghRrPQ8Q+OIguQNykF2wodw Fig 2b ------- COMMENT: d21245ab5467d47b 5 gOdbaMY+u9asYG7dR9QqL+PXwTk Fig 2 ------- COMMENT: d21245ab5467d47b 6 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: d21245ab5467d47b 7 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: d21245ab5467d47b 8 OOKOYrMc7BbgkQPciuCnfirQS58 fig3. ------- COMMENT: d21f20d1d014ccba 6 UnQw94nU5T+QzGDesDT6Zls7XHA fig 7B ------- COMMENT: d21f20d1d014ccba 8 l165mgamoT9Y90wthcjZQM/bn5E table 1 ------- COMMENT: d21f20d1d014ccba 9 l165mgamoT9Y90wthcjZQM/bn5E table 1 ------- COMMENT: d21f20d1d014ccba 10 gm1Px94b5UBdjxqoHSq63NZdxOQ table1 ------- COMMENT: d21f20d1d014ccba 11 gm1Px94b5UBdjxqoHSq63NZdxOQ table1 ------- COMMENT: d21f630f6100c4d0 1 hk/5LMotjOY5ETP0Yb7X7ZBJqTE figure 1 (comment: rap1 intron2, ftp105 intron 3 and pyp3 intron 1) ------- COMMENT: d21f630f6100c4d0 2 W9VeTu5T6JkIrTGFU/01lEWodfc Among the mutants studied, Δcay1 and Δtls1 strains also showed splicing defects spe- cific for rap1 intron 2 (Supplementary Figure S10A). ------- COMMENT: d21f630f6100c4d0 3 W9VeTu5T6JkIrTGFU/01lEWodfc Among the mutants studied, Δcay1 and Δtls1 strains also showed splicing defects spe- cific for rap1 intron 2 (Supplementary Figure S10A). ------- COMMENT: d21f630f6100c4d0 5 7x4+Aq5HE3wM3PNuiehoxoMMs3w Figure S13C (comment: A shorter form of the protein translated from intron-retained transcript) ------- COMMENT: d21f630f6100c4d0 6 7x4+Aq5HE3wM3PNuiehoxoMMs3w Figure S13C (comment: A shorter form of the protein translated from intron-retained transcript) ------- COMMENT: d21f630f6100c4d0 7 muo8AzaSFDlXR7LdHz//4LLWCFc (comment: A shorter form of the protein translated from intron-retained transcript) ------- COMMENT: d21f630f6100c4d0 8 muo8AzaSFDlXR7LdHz//4LLWCFc (comment: A shorter form of the protein translated from intron-retained transcript) ------- COMMENT: d21f630f6100c4d0 9 muo8AzaSFDlXR7LdHz//4LLWCFc (comment: A shorter form of the protein translated from intron-retained transcript) ------- COMMENT: d21f630f6100c4d0 10 muo8AzaSFDlXR7LdHz//4LLWCFc (comment: A shorter form of the protein translated from intron-retained transcript) ------- COMMENT: d21f630f6100c4d0 11 muo8AzaSFDlXR7LdHz//4LLWCFc (comment: A shorter form of the protein translated from intron-retained transcript) ------- COMMENT: d21f630f6100c4d0 12 muo8AzaSFDlXR7LdHz//4LLWCFc (comment: A shorter form of the protein translated from intron-retained transcript) ------- COMMENT: d21f630f6100c4d0 13 muo8AzaSFDlXR7LdHz//4LLWCFc (comment: A shorter form of the protein translated from intron-retained transcript) ------- COMMENT: d21f630f6100c4d0 14 muo8AzaSFDlXR7LdHz//4LLWCFc (comment: A shorter form of the protein translated from intron-retained transcript) ------- COMMENT: d21f630f6100c4d0 15 muo8AzaSFDlXR7LdHz//4LLWCFc (comment: A shorter form of the protein translated from intron-retained transcript) ------- COMMENT: d21f630f6100c4d0 16 muo8AzaSFDlXR7LdHz//4LLWCFc (comment: A shorter form of the protein translated from intron-retained transcript) ------- COMMENT: d21f630f6100c4d0 18 muo8AzaSFDlXR7LdHz//4LLWCFc (comment: A shorter form of the protein translated from intron-retained transcript) ------- COMMENT: d21f630f6100c4d0 19 muo8AzaSFDlXR7LdHz//4LLWCFc (comment: A shorter form of the protein translated from intron-retained transcript) ------- COMMENT: d21f630f6100c4d0 20 muo8AzaSFDlXR7LdHz//4LLWCFc (comment: A shorter form of the protein translated from intron-retained transcript) ------- COMMENT: d21f630f6100c4d0 21 zKB7V9+qC4OZ3BZfHdGu2UsnWsU (comment: Semi quantitative RT-PCR followed by gel electrophoresis); Intron retention observed in absence of sde2, cay1 and tls1 ------- COMMENT: d21f630f6100c4d0 22 zKB7V9+qC4OZ3BZfHdGu2UsnWsU (comment: Semi quantitative RT-PCR followed by gel electrophoresis); Intron retention observed in absence of sde2, cay1 and tls1 ------- COMMENT: d21f630f6100c4d0 23 wWgkZva67aV5aIQBFT5WXKBkVUE (Supplementary Figures S6 and S7A). ...the disruption of secondary structures by exposure to elevated temperatures could open the fold and increase the distance between the BP and 3′ss.... Indeed, excision of introns with secondary structures such as rap1 intron 2, whi5 intron 1, atg20 intron 2 and mug65 intron 2 was sensitive to 15 min treatment at 42◦C. After lower- ing the temperature to 25◦C, splicing defects of these in- trons recovered in wt cells, but the recovery with whi5 and atg20 introns was slower in Δsde2. ------- COMMENT: d21f630f6100c4d0 25 ywLQ9rVS6+uTKgjFyEUZxwzOTB4 (Supplementary Figures S6 and S7A). (comment: rap1 intron 2) ------- COMMENT: d21f630f6100c4d0 27 7ifQnN5H+tnljD5EaQAF8DmSNRM Figure S12B ------- COMMENT: d21f630f6100c4d0 28 7ifQnN5H+tnljD5EaQAF8DmSNRM Figure S12B ------- COMMENT: d21f630f6100c4d0 29 7ifQnN5H+tnljD5EaQAF8DmSNRM Figure S12B ------- COMMENT: d21f630f6100c4d0 33 aSH1UoHehaOSoogIzECd67T4pn4 figure 1 ------- COMMENT: d21f630f6100c4d0 34 aSH1UoHehaOSoogIzECd67T4pn4 figure 1 ------- COMMENT: d21f630f6100c4d0 35 aSH1UoHehaOSoogIzECd67T4pn4 figure 1 ------- COMMENT: d21f630f6100c4d0 37 uBSlvagB43MzQtGfZ7+GGW8xzCI figure 1 (rap1 intron2 branch site distance decreased) ------- COMMENT: d21f630f6100c4d0 38 xvdczmx1h6uex00FpedCpBrsV2s Figure S13C ------- COMMENT: d21f630f6100c4d0 39 xvdczmx1h6uex00FpedCpBrsV2s Figure S13C ------- COMMENT: d21f630f6100c4d0 40 xvdczmx1h6uex00FpedCpBrsV2s Figure S13C ------- COMMENT: d21f630f6100c4d0 41 xvdczmx1h6uex00FpedCpBrsV2s Figure S13C ------- COMMENT: d21f630f6100c4d0 42 xvdczmx1h6uex00FpedCpBrsV2s Figure S13C ------- COMMENT: d21f630f6100c4d0 43 xvdczmx1h6uex00FpedCpBrsV2s Figure S13C ------- COMMENT: d21f630f6100c4d0 44 xvdczmx1h6uex00FpedCpBrsV2s Figure S13C ------- COMMENT: d21f630f6100c4d0 45 xvdczmx1h6uex00FpedCpBrsV2s Figure S13C ------- COMMENT: d21f630f6100c4d0 46 xvdczmx1h6uex00FpedCpBrsV2s Figure S13C ------- COMMENT: d21f630f6100c4d0 47 xvdczmx1h6uex00FpedCpBrsV2s Figure S13C ------- COMMENT: d21f630f6100c4d0 48 xvdczmx1h6uex00FpedCpBrsV2s Figure S13C ------- COMMENT: d21f630f6100c4d0 49 hk/5LMotjOY5ETP0Yb7X7ZBJqTE figure 1 (comment: rap1 intron2, ftp105 intron 3 and pyp3 intron 1) ------- COMMENT: d21f630f6100c4d0 50 hk/5LMotjOY5ETP0Yb7X7ZBJqTE figure 1 (comment: rap1 intron2, ftp105 intron 3 and pyp3 intron 1) ------- COMMENT: d24a14abb87caf09 1 PjweyuMsOViUTG+vrmMS63mIX2c Fig4B. DAPI staining. (comment: CHECK I have requested a new term and included response to streptonigrin but should it be more general e.g. response to DNA damaging agent (if this is known)) ------- COMMENT: d24a14abb87caf09 2 vNeVjF43B1AHoBr9wanGNrQ1nK4 Fig4D. DAPI staining. (comment: CHECK I have requested a new term and included response to streptonigrin but should it be more general e.g. response to DNA damaging agent (if this is known)) ------- COMMENT: d24a14abb87caf09 3 l9NYmdZb8Apz4z5h6b+Lf8dKtoM Fig4C. DAPI staining. (comment: CHECK I have requested a new term and included response to streptonigrin but should it be more general e.g. response to DNA damaging agent (if this is known)) ------- COMMENT: d272c70052e00b9c 2 FV1qn2ScCy2LpYTAfI4koSO+CP0 (comment: CHECK 40 fold less) ------- COMMENT: d2ab88a6fe77fa6d 1 gkJ0XdMYJYAWqDF5xmSOAoB25u0 Using this system, we observed that SpHsp90-EA supported viability of Sz. pombe cells (Fig. 1b). ------- COMMENT: d2ab88a6fe77fa6d 2 Pfi5cW4Ighswi3rsh0yuigZIPZ0 Sz. pombe cells expressing SpHsp90-EA had an osmolyte-remediated temperature sensitivity phenotype, which strongly suggested conservation of EA-specific phenotypes (Fig. 3b). ------- COMMENT: d2ab88a6fe77fa6d 3 Pfi5cW4Ighswi3rsh0yuigZIPZ0 Sz. pombe cells expressing SpHsp90-EA had an osmolyte-remediated temperature sensitivity phenotype, which strongly suggested conservation of EA-specific phenotypes (Fig. 3b). ------- COMMENT: d2ab88a6fe77fa6d 4 38d1MaH8IFMQzgfdZTQDnp6Idvg The suppressive effect of TA and EK on EA defects in non-essential functions were not specific to S. cerevisiae, as these mutations also rescued SpHsp90-EA mediated temperature sensitivity in Sz. pombe (Fig. 5d). ------- COMMENT: d2ab88a6fe77fa6d 5 38d1MaH8IFMQzgfdZTQDnp6Idvg The suppressive effect of TA and EK on EA defects in non-essential functions were not specific to S. cerevisiae, as these mutations also rescued SpHsp90-EA mediated temperature sensitivity in Sz. pombe (Fig. 5d). ------- COMMENT: d2d1a8fb3af2e67e 5 pUH838ttUUDBtkTqEbaHGJe+4m8 Interestingly, the DC mutant with the deletion of the whole C-terminus between amino acid 498 and 648 was resistant to HU and MMS almost like the wild type cells (Fig. 1D and 2B). The only difference we could readily find for the DC mutant was that the protein level was higher than in the wild type cells (Fig. 2C), suggesting that the C-terminus may not contain a robust AAD (see below). ------- COMMENT: d2d1a8fb3af2e67e 14 hGkGWfQWdSyokY6SNQj8JyEld2w Fig. 1D, Fig. 2B ------- COMMENT: d2d1a8fb3af2e67e 15 kFOPRHQrTsATcFxFZzaC2KQWyMQ Fig. 1D,Fig. 2B ------- COMMENT: d2d1a8fb3af2e67e 16 26IPEgtpLQDbYGhd/m+Y0wNgOZM Fig. 1D ------- COMMENT: d2d1a8fb3af2e67e 18 rxTHSWsuaVL20MOI6Nf2mcq0B8w We found that combinations of the previously reported E368K mutation [47] with K56R or F303S were lethal suggesting a defect in DNA replication. ------- COMMENT: d2d1a8fb3af2e67e 19 rxTHSWsuaVL20MOI6Nf2mcq0B8w We found that combinations of the previously reported E368K mutation [47] with K56R or F303S were lethal suggesting a defect in DNA replication. ------- COMMENT: d2d1a8fb3af2e67e 21 hGkGWfQWdSyokY6SNQj8JyEld2w Fig. 1D, Fig. 2B ------- COMMENT: d2d1a8fb3af2e67e 22 tTwgMgeRaddFS8EbvCRHrwzBNT8 Fig. 1D, Fig. 2B ------- COMMENT: d2d1a8fb3af2e67e 23 kFOPRHQrTsATcFxFZzaC2KQWyMQ Fig. 1D,Fig. 2B ------- COMMENT: d2d1a8fb3af2e67e 24 kFOPRHQrTsATcFxFZzaC2KQWyMQ Fig. 1D,Fig. 2B ------- COMMENT: d2d1a8fb3af2e67e 25 26IPEgtpLQDbYGhd/m+Y0wNgOZM Fig. 1D ------- COMMENT: d2d1a8fb3af2e67e 26 26IPEgtpLQDbYGhd/m+Y0wNgOZM Fig. 1D ------- COMMENT: d2d1a8fb3af2e67e 30 aClKtruK7YOJABTAFKsSDfEzi+s C13Y and K56R mutations completely eliminated the phosphorylation of Chk1 in MMS-treated cells (Fig. 3A) ------- COMMENT: d2d1a8fb3af2e67e 31 aClKtruK7YOJABTAFKsSDfEzi+s C13Y and K56R mutations completely eliminated the phosphorylation of Chk1 in MMS-treated cells (Fig. 3A) ------- COMMENT: d2d1a8fb3af2e67e 32 BY4rfCPcbFJ7eh4yMVPS0aqZjVM C13Y and K56R mutations completely eliminated the phosphorylation of Chk1 in MMS-treated cells (Fig. 3A)The slight decrease in Cds1 phosphorylation may be caused indirectly by a minor defect in DNA replication ------- COMMENT: d2d1a8fb3af2e67e 33 aClKtruK7YOJABTAFKsSDfEzi+s C13Y and K56R mutations completely eliminated the phosphorylation of Chk1 in MMS-treated cells (Fig. 3A) ------- COMMENT: d2d1a8fb3af2e67e 34 ZP6F15bYwu2llNvAt7NFKkosREs Fig. 3D ------- COMMENT: d2d1a8fb3af2e67e 35 ZP6F15bYwu2llNvAt7NFKkosREs Fig. 3D ------- COMMENT: d2d1a8fb3af2e67e 36 ZP6F15bYwu2llNvAt7NFKkosREs Fig. 3D ------- COMMENT: d2d1a8fb3af2e67e 37 ZP6F15bYwu2llNvAt7NFKkosREs Fig. 3D ------- COMMENT: d2d1a8fb3af2e67e 38 YZquAp3aUoJTpq8Lphm0W6od8RU fig3 ------- COMMENT: d2d1a8fb3af2e67e 39 YZquAp3aUoJTpq8Lphm0W6od8RU fig3 ------- COMMENT: d2d1a8fb3af2e67e 40 xsFxQJUrRfU8R2Zvc0Jkh377/DY The C13Y and K56R mutations abolished the scaffolding function of Rad4 required for the activation of Chk1 but not Rad3 ------- COMMENT: d2d1a8fb3af2e67e 41 QYxvuffN9zPYpELW/h1uD0NaOSI C13Y-K56R mutation abolished the interaction with Crb2 (Fig. 5C), not Rad9 (Fig. 5A and B). ------- COMMENT: d2d1a8fb3af2e67e 42 5z/YKKUBMEsi8LrUIW3LbzaSWR0 E368K mutation abolished the binding to Rad9 as previously reported [47] (Fig. 5A and B) ------- COMMENT: d2d1a8fb3af2e67e 43 QYxvuffN9zPYpELW/h1uD0NaOSI C13Y-K56R mutation abolished the interaction with Crb2 (Fig. 5C), not Rad9 (Fig. 5A and B). ------- COMMENT: d2d1a8fb3af2e67e 44 90HXwurRcRftJwosA2gWKFVLhIM Consistent with the previous report [58], the interaction between Rad9 and Rad4 was dependent on Rad9 phosphorylation because the phosphorylation site mutant Rad9-T412A could not pull-down Rad4 (Fig. 5A, first lane on the left) and the interaction between Rad9 and Rad4 was sensitive to l-phosphatase treatment (Fig. S5A). ------- COMMENT: d2d1a8fb3af2e67e 45 LR03zDKtXw9LS9E+z8jX9MTPg+E Fig. 5A and B ------- COMMENT: d2d1a8fb3af2e67e 47 EUvsEPaArGVTWxNh0QEm5Yk5HAg Fig. 3a, b ------- COMMENT: d2d1a8fb3af2e67e 48 KwKrUQBiod6ZmgoB9zRxCIBemSY Fig. 3a, b. (comment: CHECK cds1-T11) ------- COMMENT: d2d1a8fb3af2e67e 49 EUvsEPaArGVTWxNh0QEm5Yk5HAg Fig. 3a, b ------- COMMENT: d2d1a8fb3af2e67e 50 cdZZlQfEZ2MifRdhi3dcoOd1uII (comment: CHECK affected by rad4) ------- COMMENT: d2d1a8fb3af2e67e 52 b/g6FINGyOS/ldA1NCKMePNfGOk (comment: CHECK phosphorylated rad9) ------- COMMENT: d2e620f2641d569b 2 ES4cWgjeLCMXUQa/7eMTkHVqEIk (comment: CHECK activated_by(CHEBI:18420)) ------- COMMENT: d30173fb3bf67d59 8 06RGRv6eMFjM1IQPXon6kF19DP8 Fig 2B ------- COMMENT: d30173fb3bf67d59 11 06RGRv6eMFjM1IQPXon6kF19DP8 Fig 2B ------- COMMENT: d30173fb3bf67d59 16 iRvAcjzjUT22yPIgSdDZB3dYw+w (comment: CONDITION 0.75 M) ------- COMMENT: d30173fb3bf67d59 17 iRvAcjzjUT22yPIgSdDZB3dYw+w (comment: CONDITION 0.75 M) ------- COMMENT: d30203723a93539d 1 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d30203723a93539d 2 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: d30203723a93539d 3 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: d30203723a93539d 5 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: d30203723a93539d 8 kO8/2qJENOoJ1pYseqlTOvQU2Bo enclosure arrow in Figs 4Ci,ii) ------- COMMENT: d30203723a93539d 10 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: d30dca7bfe4b2d3d 6 Sp2RSV6T4x0pYGI3gPZZNsHAPzI (comment: protein-coding genes and intergenic regions) ------- COMMENT: d30dca7bfe4b2d3d 7 Sp2RSV6T4x0pYGI3gPZZNsHAPzI (comment: protein-coding genes and intergenic regions) ------- COMMENT: d30dca7bfe4b2d3d 8 Sp2RSV6T4x0pYGI3gPZZNsHAPzI (comment: protein-coding genes and intergenic regions) ------- COMMENT: d30dca7bfe4b2d3d 9 Sp2RSV6T4x0pYGI3gPZZNsHAPzI (comment: protein-coding genes and intergenic regions) ------- COMMENT: d30dca7bfe4b2d3d 10 Sp2RSV6T4x0pYGI3gPZZNsHAPzI (comment: protein-coding genes and intergenic regions) ------- COMMENT: d30dca7bfe4b2d3d 11 Sp2RSV6T4x0pYGI3gPZZNsHAPzI (comment: protein-coding genes and intergenic regions) ------- COMMENT: d30dca7bfe4b2d3d 12 POQMQp/FFkLNn+X+D//kyYOh3MY (comment: assayed in intergenic regions) ------- COMMENT: d30dca7bfe4b2d3d 13 POQMQp/FFkLNn+X+D//kyYOh3MY (comment: assayed in intergenic regions) ------- COMMENT: d30dca7bfe4b2d3d 14 POQMQp/FFkLNn+X+D//kyYOh3MY (comment: assayed in intergenic regions) ------- COMMENT: d30dca7bfe4b2d3d 15 POQMQp/FFkLNn+X+D//kyYOh3MY (comment: assayed in intergenic regions) ------- COMMENT: d30dca7bfe4b2d3d 16 Sp2RSV6T4x0pYGI3gPZZNsHAPzI (comment: protein-coding genes and intergenic regions) ------- COMMENT: d30dca7bfe4b2d3d 19 Sp2RSV6T4x0pYGI3gPZZNsHAPzI (comment: protein-coding genes and intergenic regions) ------- COMMENT: d30dca7bfe4b2d3d 20 Sp2RSV6T4x0pYGI3gPZZNsHAPzI (comment: protein-coding genes and intergenic regions) ------- COMMENT: d30dca7bfe4b2d3d 21 Sp2RSV6T4x0pYGI3gPZZNsHAPzI (comment: protein-coding genes and intergenic regions) ------- COMMENT: d30dca7bfe4b2d3d 22 Sp2RSV6T4x0pYGI3gPZZNsHAPzI (comment: protein-coding genes and intergenic regions) ------- COMMENT: d30dca7bfe4b2d3d 23 Sp2RSV6T4x0pYGI3gPZZNsHAPzI (comment: protein-coding genes and intergenic regions) ------- COMMENT: d30dca7bfe4b2d3d 50 Ltl9sDTr79ANTtXpJHof6GAx4NY Sir2 and Clr3 act cooperatively upon histone H3 at K9/K14 throughout the genome, including all the silent regions (rDNA, centromeres, mat2/3 and telomeres). ------- COMMENT: d335f8eb4efaf35d 1 AJM3UzZfcNA51z7jraSDCDOL5LY Fig. S1B ------- COMMENT: d335f8eb4efaf35d 2 AJM3UzZfcNA51z7jraSDCDOL5LY Fig. S1B ------- COMMENT: d335f8eb4efaf35d 3 f492DpVbvxvka7nb6jD1d1zh/X4 Figure 7B We found that DTT-induced increase of Epr1 was severely diminished in ire1D (Figure 7B), indicating that Epr1 upregulation requires Ire1. ------- COMMENT: d335f8eb4efaf35d 4 NWQ0FMKyS3MZhZESZmyzW8Mzi7s Figure S1E ------- COMMENT: d335f8eb4efaf35d 5 ADDuxZftN5pqw4TdnjnEFabq06w Figure S1E nitrogen starvation-induced ER-phagy appeared to be normal in epr1D ------- COMMENT: d335f8eb4efaf35d 6 FmYGSaI1qpMZpgAxjOcVbgZQIMg Figure 2B, 2G, 2H ------- COMMENT: d335f8eb4efaf35d 7 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: d335f8eb4efaf35d 9 FDZfzUZC1Usx4Ohehbb9V9oRFl8 (Figure S6B), indicating that Ire1 is dispensable for DTT-induced bulk autophagy but is essential for DTT-induced ER-phagy. ------- COMMENT: d335f8eb4efaf35d 12 UtzUywHB1vil+X1HiAvTwe8iTQM (Figure 5A) reduced in scs2D and abolished in scs2D scs22D ------- COMMENT: d335f8eb4efaf35d 15 cJtnlEVdp7+rzEycWyo5w+fCG08 Figure 3C Epr1 interacted with both Scs2 and Scs22 in the Y2H assay ------- COMMENT: d335f8eb4efaf35d 17 WXWvWshB3EFhh4ms65XjEXXDCYM Figures 1B and S1C ------- COMMENT: d335f8eb4efaf35d 18 Jk8L9f4ComAz6/e7ZGUzFSU7/WI Figures 2G and 2H ------- COMMENT: d335f8eb4efaf35d 19 tN/dyDxxphb/QcUkcoQZMj5eZUA Figure 2a ------- COMMENT: d335f8eb4efaf35d 45 WXWvWshB3EFhh4ms65XjEXXDCYM Figures 1B and S1C ------- COMMENT: d335f8eb4efaf35d 49 2urL3Wj6zxlQ6fohTo0Ynf5SzuI Figure 3C The 42-amino-acid Epr1-C region (residues 339–380), which is capable of Atg8 bind- ing and contains the predicted FFAT motif, is sufficient for inter- acting with VAPs ------- COMMENT: d335f8eb4efaf35d 51 hXPZ9CtOptLM3oeHTJptNEcCogU Figure 3D (comment: CHECK ******check with DAN, is this an overexpression allele?) ------- COMMENT: d335f8eb4efaf35d 52 bQrAvw5v3pdeGEjTasNZSNQl2dw Figure 6A ------- COMMENT: d335f8eb4efaf35d 53 bQrAvw5v3pdeGEjTasNZSNQl2dw Figure 6A ------- COMMENT: d335f8eb4efaf35d 54 bQrAvw5v3pdeGEjTasNZSNQl2dw Figure 6A ------- COMMENT: d335f8eb4efaf35d 55 bQrAvw5v3pdeGEjTasNZSNQl2dw Figure 6A ------- COMMENT: d335f8eb4efaf35d 56 bQrAvw5v3pdeGEjTasNZSNQl2dw Figure 6A ------- COMMENT: d335f8eb4efaf35d 57 bQrAvw5v3pdeGEjTasNZSNQl2dw Figure 6A ------- COMMENT: d335f8eb4efaf35d 58 AJM3UzZfcNA51z7jraSDCDOL5LY Fig. S1B ------- COMMENT: d335f8eb4efaf35d 59 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: d335f8eb4efaf35d 60 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: d335f8eb4efaf35d 61 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: d335f8eb4efaf35d 62 tN/dyDxxphb/QcUkcoQZMj5eZUA Figure 2a ------- COMMENT: d335f8eb4efaf35d 63 TR+QfyJxfW2z+2rWcPQyxEqvZTQ (comment: CHECK check with Dan ****) Thus, Epr1 is an ER-phagy receptor required for ER stress-induced selective autophagy of both the nuclear envelope and the peripheral ER. ------- COMMENT: d335f8eb4efaf35d 64 2urL3Wj6zxlQ6fohTo0Ynf5SzuI Figure 3C The 42-amino-acid Epr1-C region (residues 339–380), which is capable of Atg8 bind- ing and contains the predicted FFAT motif, is sufficient for inter- acting with VAPs ------- COMMENT: d335f8eb4efaf35d 65 2urL3Wj6zxlQ6fohTo0Ynf5SzuI Figure 3C The 42-amino-acid Epr1-C region (residues 339–380), which is capable of Atg8 bind- ing and contains the predicted FFAT motif, is sufficient for inter- acting with VAPs ------- COMMENT: d335f8eb4efaf35d 66 4grpyXqCWFfmJVxqQLWlyg8bwCw (Figure 3D) As expected, in cells lacking both Scs2 and Scs22, Epr1 became diffusely distributed in the cytoplasm ------- COMMENT: d335f8eb4efaf35d 67 WR4eY9sTthO3PnzjKXFbZFO6hx0 figure 3D ------- COMMENT: d335f8eb4efaf35d 68 iCflUse/4qBOiVMVzCyB2TtuncI Figures S3B and S3D Epr1-C showed ER localization in vegetatively growing cells, whereas Epr1-N was diffusely distributed in the cytoplasm and the nucleus ------- COMMENT: d335f8eb4efaf35d 69 iCflUse/4qBOiVMVzCyB2TtuncI Figures S3B and S3D Epr1-C showed ER localization in vegetatively growing cells, whereas Epr1-N was diffusely distributed in the cytoplasm and the nucleus ------- COMMENT: d335f8eb4efaf35d 71 kOgc6ne82F9j+5HKOO+eSrK75A8 Remarkably, Epr1-C, but not Epr1-N, could completely rescue the defects of epr1D in DTT-induced ER- phagy ------- COMMENT: d335f8eb4efaf35d 72 d5WCU9MwsHm3HbT0ctkPxP1zPu4 Mutating the FFAT motif or the AIM abolished the ability of Epr1-C to rescue epr1D (Figures 4A, 4B, and S3D). ------- COMMENT: d335f8eb4efaf35d 73 d5WCU9MwsHm3HbT0ctkPxP1zPu4 Mutating the FFAT motif or the AIM abolished the ability of Epr1-C to rescue epr1D (Figures 4A, 4B, and S3D). ------- COMMENT: d335f8eb4efaf35d 75 aBGYLSDIe87oHbmV8f0cF+dAkTg Figure 1C AIM-mutated Epr1- C was pulled down as efficiently as wild-type Epr1-C by Scs2 but did not support the pull-down of Atg8 PLUS more experiments We hypothesized that the main role of Epr1 in ER-phagy is to mediate a connection between Atg8 and VAPs. requirement of Epr1 in ER-phagy can be by- passed by an artificial soluble tether that bridges an Atg8-VAP connection.Figure 4D). ------- COMMENT: d335f8eb4efaf35d 78 UtzUywHB1vil+X1HiAvTwe8iTQM (Figure 5A) reduced in scs2D and abolished in scs2D scs22D ------- COMMENT: d335f8eb4efaf35d 80 f75rTWBnUsKspBkchc4dLoq0Wdo fig 6b ------- COMMENT: d335f8eb4efaf35d 81 bQrAvw5v3pdeGEjTasNZSNQl2dw Figure 6A ------- COMMENT: d335f8eb4efaf35d 82 f75rTWBnUsKspBkchc4dLoq0Wdo fig 6b ------- COMMENT: d335f8eb4efaf35d 84 K/sW1Opks2jYc6R+XBAmpkWfoqg Figure 7E the ER-phagy defect of ire1D was largely rescued (Figure 7E). ------- COMMENT: d34af2b4410c9977 2 1yVvQjwRje2DNirBYv6zMzmh0hY Localization depends on Cdc2 kinase activity but not on Clp1. ------- COMMENT: d34af2b4410c9977 4 QNFaguzH18YDE/WsmON32mSZZXg (comment: The phenotype can be seen at 32 degree.) ------- COMMENT: d34af2b4410c9977 5 i12o2cs7D+JBv9VKdsSd71zjXRM Loss of viability is evident only when cells are exposed to 32 degree before and upon entry into stationary phase. ------- COMMENT: d34af2b4410c9977 6 U9oc+iec+cO9CM0PkfbiuVFbruc Cell length increases during log and stationary phases at 32 degree. ------- COMMENT: d34af2b4410c9977 8 S1wzbSJ6d5OvcN4JLw3iBEYGwyA The N/C ratio was also reduced but not significantly different from that of stationary phase cells (Fig. 1E; Fig. S1G), suggesting that the observed nuclear size differences from stationary phase originate from the cell size differences. ------- COMMENT: d34af2b4410c9977 9 zTsZKwO7cyapRF9dcD1Ni5M/3IA (comment: The phenotype can be seen at 32˚C. The phenotype can be seen at 32˚C.) Furthermore, the log phase chromosomes more actively fluctuated in cdc2-L7 cells than in WT cells, and despite a repression in chromosome fluctuation, fluctuation was still elevated in the stationary phase, as demonstrated by an upward shift of the cdc2-L7 MSD plot of the ade6 locus and a complete lack of an overlap of their 95% confidence intervals with those of the WT plot (Fig. 5H). ------- COMMENT: d351bbb0a4c9383c 81 +AwkjG2n27RVcaEoSPUwjAKmhqk (comment: CHECK nse3(1-190aa), 0.5 mM 3-AT) ------- COMMENT: d351bbb0a4c9383c 82 jx/RAcOdS1kV5Z0JJj1rYmSu3Xw (comment: CHECK nse3(200-307aa), 0.5mM 3-AT) ------- COMMENT: d351bbb0a4c9383c 83 1v3wTG/1crZ4TzizZhmhuMw2oqE (comment: CHECK nse2(2-178aa), 0.5mM 3-AT) ------- COMMENT: d351bbb0a4c9383c 84 Kulcev4UKh5vNZld10oqbVtoGds (comment: CHECK smc5 (CC region), 10mM 3-AT) ------- COMMENT: d351bbb0a4c9383c 86 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: d351bbb0a4c9383c 87 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: d351bbb0a4c9383c 88 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: d351bbb0a4c9383c 89 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: d351bbb0a4c9383c 90 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: d351bbb0a4c9383c 91 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: d351bbb0a4c9383c 92 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: d351bbb0a4c9383c 93 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: d351bbb0a4c9383c 94 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: d351bbb0a4c9383c 95 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: d351bbb0a4c9383c 98 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: d351bbb0a4c9383c 99 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: d351bbb0a4c9383c 102 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: d351bbb0a4c9383c 103 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: d351bbb0a4c9383c 104 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: d351bbb0a4c9383c 105 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: d351bbb0a4c9383c 106 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: d351bbb0a4c9383c 107 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: d351bbb0a4c9383c 187 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: d3646296251ff27d 1 PxGYNeSxrV5PD+XotSQQNBxfnSo fig 1a ------- COMMENT: d3646296251ff27d 2 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: d3646296251ff27d 3 PxGYNeSxrV5PD+XotSQQNBxfnSo fig 1a ------- COMMENT: d3646296251ff27d 4 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: d3646296251ff27d 5 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: d3646296251ff27d 7 xK236mXgYloo+mx4w8mXINu2p+I Fig. 4c ------- COMMENT: d3646296251ff27d 8 G2RTiSRzpGfQc3LUXrBavCAxZHo Fig. 4 ------- COMMENT: d3646296251ff27d 9 G2RTiSRzpGfQc3LUXrBavCAxZHo Fig. 4 ------- COMMENT: d3646296251ff27d 10 G2RTiSRzpGfQc3LUXrBavCAxZHo Fig. 4 ------- COMMENT: d3646296251ff27d 11 +2XFMV1MQ4r+teF637sQosvJlkk fig 7a ------- COMMENT: d3646296251ff27d 12 +2XFMV1MQ4r+teF637sQosvJlkk fig 7a ------- COMMENT: d3646296251ff27d 13 J10Cw4+4tH4xHHQI0Sbdm3mbMAE Figure 7 ------- COMMENT: d3646296251ff27d 14 J10Cw4+4tH4xHHQI0Sbdm3mbMAE Figure 7 ------- COMMENT: d3646296251ff27d 15 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: d378014af8ea8779 51 aXW485+NN2j6WS/A7JxklhZK3AU (comment: unspecfied RxxS site(s)) ------- COMMENT: d378014af8ea8779 52 aXW485+NN2j6WS/A7JxklhZK3AU (comment: unspecfied RxxS site(s)) ------- COMMENT: d384a52dcc0dc8a1 12 Exhcc+EgDsq9Qcu3bL0NtMpMcxo Surprisingly, the S. pombe capping enzyme subunits do not interact with each other. ------- COMMENT: d384a52dcc0dc8a1 13 Exhcc+EgDsq9Qcu3bL0NtMpMcxo Surprisingly, the S. pombe capping enzyme subunits do not interact with each other. ------- COMMENT: d3a483f9bb7842f8 1 wpbSFH6pV3UPiGTKpcWKLpdwxso (Figure ID, -Thi, Dis2),which allows for colony formation.Cells producing the mutant T316A protein under the strong promoter, on the other hand,severely blocked colony formation(Figure ID,-Thi,Dis2T316A), ------- COMMENT: d3a483f9bb7842f8 2 WT35fdt9ZHbsqr4qEaHeNP3yXyY A temperatureshift(G2blockandrelease)experi- ment using the cdc25-22 mutant showed that the level of T316-phosphorylatedDis2peakedinmitosis(Figure2A). ------- COMMENT: d3a483f9bb7842f8 3 WDnX3Tu3xk2aEQqqrL+luFvHin8 figure 3A because the spindle elongates in the absence of sister chromatid separation. ------- COMMENT: d3a483f9bb7842f8 4 5L6DHyiIh6ZZKJVEk3iEuToUv9c figure 3A However, dis2-11displayedan allele-specificmitoticphenotypewith spindle elongation (Ohkura et al., 1989; see Figure 3A). ------- COMMENT: d3a483f9bb7842f8 6 lBMtxJ7UNkhoM5MQsYcIj5RxbB0 Figure 3A abnormal cell cycle arrest in mitotic metaphase, condend chrosmosmes ------- COMMENT: d3a483f9bb7842f8 7 LhWEhRLOAieIqBVz1cn5kqXW0Bg figure 3A ------- COMMENT: d3a483f9bb7842f8 8 r7H1OswGbh9um+z0QJ0qF3GlBGU In parallel with the increase in plasmid loss, cells containing con- densed chromosomes with a short metaphase spindle dramatically increased (Figure 3C). After transferring the culture from selective to non-selective medium at 33°C, the fraction of these metaphase-arrested cells increased about nine times within 20 h (from 2 to 19%). We concluded, therefore, that the complete absence of PPI in fission yeast leads to the metaphase arrest, which is strikingly similar to the phenotype of sds22 mutants. More than 90% of the cells examined contained condensed chromosomes (Figure 3B, upper panel). ------- COMMENT: d3a483f9bb7842f8 11 lBMtxJ7UNkhoM5MQsYcIj5RxbB0 Figure 3A abnormal cell cycle arrest in mitotic metaphase, condend chrosmosmes ------- COMMENT: d3a483f9bb7842f8 12 bXZdZHgCmhjqdOphFFE/izXZpJ0 A multicopy plasmid which conferred the ability to suppress ts sds22-181 was isolated. ------- COMMENT: d3a483f9bb7842f8 17 W9JZV94WbaT5j8Inw8ag1QGdOUQ Heterozygous diploids verified by Southern hybridization (lower panel) yielded four viable spores at 26 or 33°C with Ade+ segregating 2+:2-, indicating that the sds23+ gene was not essential for viability. Colonies were not formed at 22 or 36°C, however (Figure 6B), indicating that the gene-disrupted Asds23 causes cold and temperature sensitivity. ------- COMMENT: d3a483f9bb7842f8 18 W9JZV94WbaT5j8Inw8ag1QGdOUQ Heterozygous diploids verified by Southern hybridization (lower panel) yielded four viable spores at 26 or 33°C with Ade+ segregating 2+:2-, indicating that the sds23+ gene was not essential for viability. Colonies were not formed at 22 or 36°C, however (Figure 6B), indicating that the gene-disrupted Asds23 causes cold and temperature sensitivity. ------- COMMENT: d3a483f9bb7842f8 19 W9JZV94WbaT5j8Inw8ag1QGdOUQ Heterozygous diploids verified by Southern hybridization (lower panel) yielded four viable spores at 26 or 33°C with Ade+ segregating 2+:2-, indicating that the sds23+ gene was not essential for viability. Colonies were not formed at 22 or 36°C, however (Figure 6B), indicating that the gene-disrupted Asds23 causes cold and temperature sensitivity. ------- COMMENT: d3a483f9bb7842f8 20 e9MbCQFuhPddL8xtkuDjCzK0N9E The cell division rates of Asds23 at the permissive temperatureswereexceedinglyslow.Thegenerationtimes inrichYPD mediumwere5.7(3.3)and4.3(2.2)hat26 and 33°C, respectively; the values in parenthesis are the generationtimesforthewild-typestrain. ------- COMMENT: d3a483f9bb7842f8 21 e9MbCQFuhPddL8xtkuDjCzK0N9E The cell division rates of Asds23 at the permissive temperatureswereexceedinglyslow.Thegenerationtimes inrichYPD mediumwere5.7(3.3)and4.3(2.2)hat26 and 33°C, respectively; the values in parenthesis are the generationtimesforthewild-typestrain. ------- COMMENT: d3b7363a1174a921 4 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: d3b7363a1174a921 7 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: d4228b03e96911f7 1 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: d4228b03e96911f7 2 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: d4228b03e96911f7 3 5UhI2/YYj7q9cgMIX2LzXC30tBU Fig. 2, Fig. 3 (cdc13 signal) ------- COMMENT: d4228b03e96911f7 4 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: d4228b03e96911f7 5 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: d4228b03e96911f7 6 mwMDDwglCyubWJr1G/HcFNrs9D0 Fig. 3 - mad2 signal. ------- COMMENT: d4228b03e96911f7 7 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: d4228b03e96911f7 8 npO+WuMeQkeEcFxwVbQqVyL9Xww Fig. 3 (comment: CHECK rescue of FYPO:0000324) ------- COMMENT: d4228b03e96911f7 9 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: d4228b03e96911f7 10 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: d4228b03e96911f7 11 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: d4228b03e96911f7 12 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: d4228b03e96911f7 13 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: d4228b03e96911f7 14 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: d4228b03e96911f7 15 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: d4228b03e96911f7 16 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: d4228b03e96911f7 17 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: d4228b03e96911f7 18 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: d4228b03e96911f7 19 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: d4228b03e96911f7 20 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: d4228b03e96911f7 21 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: d4228b03e96911f7 22 VbKN86eNs75pqAQEtL7/yC3+4vE Fig. 5 (comment: CHECK FYPO:0000417 and FYPO:0000339) ------- COMMENT: d4228b03e96911f7 23 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: d4228b03e96911f7 24 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: d4228b03e96911f7 25 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: d4228b03e96911f7 26 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: d4228b03e96911f7 27 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: d4228b03e96911f7 28 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: d4228b03e96911f7 29 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: d4228b03e96911f7 30 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: d4228b03e96911f7 31 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: d4228b03e96911f7 32 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: d4228b03e96911f7 33 0ND5/ygezKf+aijIUwfoGJ/deZA Fig. 6 (comment: FYPO:0000061) ------- COMMENT: d4228b03e96911f7 34 0ND5/ygezKf+aijIUwfoGJ/deZA Fig. 6 (comment: FYPO:0000061) ------- COMMENT: d4228b03e96911f7 35 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: d4228b03e96911f7 36 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: d4228b03e96911f7 37 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: d4228b03e96911f7 38 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: d4228b03e96911f7 39 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: d4228b03e96911f7 40 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: d4228b03e96911f7 41 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: d4228b03e96911f7 42 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: d4228b03e96911f7 43 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: d437b51c6564a32a 9 oAQJxN0m+ULu4EefQZ660auP/ug fig 2C ------- COMMENT: d437b51c6564a32a 10 oAQJxN0m+ULu4EefQZ660auP/ug fig 2C ------- COMMENT: d437b51c6564a32a 11 oAQJxN0m+ULu4EefQZ660auP/ug fig 2C ------- COMMENT: d437b51c6564a32a 12 oAQJxN0m+ULu4EefQZ660auP/ug fig 2C ------- COMMENT: d437b51c6564a32a 13 oAQJxN0m+ULu4EefQZ660auP/ug fig 2C ------- COMMENT: d437b51c6564a32a 14 nnuWhrFU7jaQ2CsXT4A21I2oLbM fig 3a ------- COMMENT: d437b51c6564a32a 15 nnuWhrFU7jaQ2CsXT4A21I2oLbM fig 3a ------- COMMENT: d437b51c6564a32a 16 nnuWhrFU7jaQ2CsXT4A21I2oLbM fig 3a ------- COMMENT: d437b51c6564a32a 17 nnuWhrFU7jaQ2CsXT4A21I2oLbM fig 3a ------- COMMENT: d437b51c6564a32a 18 nnuWhrFU7jaQ2CsXT4A21I2oLbM fig 3a ------- COMMENT: d437b51c6564a32a 21 oAQJxN0m+ULu4EefQZ660auP/ug fig 2C ------- COMMENT: d43ed7beb6c57947 2 9pbR8tN1XpFLI0W7pGriDshAU40 Fig. 3H ------- COMMENT: d43ed7beb6c57947 3 NrXfD5zVYn85WYOIwHhmQRQDokQ (comment: from later paper: We speculate that SPRTN is able to degrade DPCs to peptide adducts that are sufficiently small for efficient TLS. https://www.sciencedirect.com/science/article/pii/S1097276518309948) ------- COMMENT: d43ed7beb6c57947 5 9pbR8tN1XpFLI0W7pGriDshAU40 Fig. 3H ------- COMMENT: d445419299dddade 1 C1HI3PhxOJp8w8F+L5z6cdR7liY Lysine 105 and Lysine 106 are acetylated in an Eso1 dependent manner. Psm3 acetylation on K105 K106 contribute to counteract the cohesin release activity of Wpl1. ------- COMMENT: d445419299dddade 141 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: d461486b9fbbaa6e 16 goHP18I1tOuARO4HuSScMrnyeNc Mix of IMP evidence & a proxy assay for hydrolase function ------- COMMENT: d471b5535e0570fe 7 rTzwV+fy3QW3hdCdCJ4VeL+o7/g (comment: CHECK affecting binding to Mdb1) ------- COMMENT: d471b5535e0570fe 10 +N80rYg3GEEaxAMbZuTDlMhCJx8 (comment: CHECK affecting binding to histone H2A (hta1)) ------- COMMENT: d47ecc7ad5ebfe9e 1 8K0m0a7V+6RxG+fkj4Zb4kAMZyM Fig. 1B, 2A ------- COMMENT: d47ecc7ad5ebfe9e 2 8K0m0a7V+6RxG+fkj4Zb4kAMZyM Fig. 1B, 2A ------- COMMENT: d47ecc7ad5ebfe9e 3 a8RbYPxyb4TryQgXmYU92FlHfQM Fig. 1A and B ------- COMMENT: d47ecc7ad5ebfe9e 4 a8RbYPxyb4TryQgXmYU92FlHfQM Fig. 1A and B ------- COMMENT: d47ecc7ad5ebfe9e 5 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: d47ecc7ad5ebfe9e 6 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: d47ecc7ad5ebfe9e 7 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: d47ecc7ad5ebfe9e 8 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: d47ecc7ad5ebfe9e 9 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: d47ecc7ad5ebfe9e 10 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: d47ecc7ad5ebfe9e 11 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: d47ecc7ad5ebfe9e 12 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: d47ecc7ad5ebfe9e 13 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: d47ecc7ad5ebfe9e 14 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: d47ecc7ad5ebfe9e 15 vF+Zo+X0Dr4yyGr/TAboTwz1kaY Fig. 2D and E ------- COMMENT: d47ecc7ad5ebfe9e 16 MpML2E+8wq5nwC1YfM2LDELzzPU Fig. 2E ------- COMMENT: d47ecc7ad5ebfe9e 17 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: d47ecc7ad5ebfe9e 18 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: d47ecc7ad5ebfe9e 19 p+t5t2kGQJfvD15ZkjiCm6OpVFk Nonetheless Arb1, by itself and/or together with Arb2, is a direct inhibitor of the slicer activity of fission yeast Ago1. Fig. 4D ------- COMMENT: d47ecc7ad5ebfe9e 20 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: d47ecc7ad5ebfe9e 21 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: d47ecc7ad5ebfe9e 22 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: d47ecc7ad5ebfe9e 23 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: d47ecc7ad5ebfe9e 24 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: d47ecc7ad5ebfe9e 25 J9cRYjlXdFNSY3fEztLZnKFLXEU Fig. 5B ------- COMMENT: d47ecc7ad5ebfe9e 26 yBvpsomR9AjfFF+9HGx96YgOkkU These results indicate that the slicer activity of Ago1 is required for the in vivo conversion of double-stranded siRNA to single-stranded siRNA ------- COMMENT: d47ecc7ad5ebfe9e 27 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: d47ecc7ad5ebfe9e 28 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: d47ecc7ad5ebfe9e 29 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: d47ecc7ad5ebfe9e 30 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: d47ecc7ad5ebfe9e 31 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: d47ecc7ad5ebfe9e 32 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: d47ecc7ad5ebfe9e 33 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: d47ecc7ad5ebfe9e 34 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: d47ecc7ad5ebfe9e 35 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: d47ecc7ad5ebfe9e 36 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: d47ecc7ad5ebfe9e 37 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: d47ecc7ad5ebfe9e 38 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: d4e73d1a93ec5860 94 NaeNTwSBAGaIC1nVVZnfSdQ+N7o (comment: CHECK phosphorylated) ------- COMMENT: d4e73d1a93ec5860 95 S9ITgDfo9DNhUaAZsvXovShN5+M (comment: CHECK K9-mehtylated) ------- COMMENT: d4e73d1a93ec5860 96 NaeNTwSBAGaIC1nVVZnfSdQ+N7o (comment: CHECK phosphorylated) ------- COMMENT: d4e73d1a93ec5860 97 H8navjllgmDqYQs7EGErQrYP/pA (comment: CHECK K9 methyl;ated) ------- COMMENT: d4e73d1a93ec5860 98 NaeNTwSBAGaIC1nVVZnfSdQ+N7o (comment: CHECK phosphorylated) ------- COMMENT: d4e73d1a93ec5860 99 S9ITgDfo9DNhUaAZsvXovShN5+M (comment: CHECK K9-mehtylated) ------- COMMENT: d4eae3f3d5d8d194 9 +wefrxHUbbXt70TOksF8n/4cVa0 (comment: residue not determined, but probably Y173) ------- COMMENT: d4eae3f3d5d8d194 14 +wefrxHUbbXt70TOksF8n/4cVa0 (comment: residue not determined, but probably Y173) ------- COMMENT: d4eae3f3d5d8d194 34 0vWKBHRwG8gU6P69D6mx931OOBQ (comment: doesn't resume normally) ------- COMMENT: d505f490197e7842 2 TWKdWxZYg8K0TIbJBb2Trszh6cM (comment: for evidence, "BrdU incorporation assay evidence used in manual assertion" (ECO:0001155) would be applicable.) ------- COMMENT: d505f490197e7842 7 TWKdWxZYg8K0TIbJBb2Trszh6cM (comment: for evidence, "BrdU incorporation assay evidence used in manual assertion" (ECO:0001155) would be applicable.) ------- COMMENT: d51e46afca090869 16 4ul+hSDesrNwFI9iWxn76vyPX1U (comment: CHECK positive regulation of meiotic cell cycle exit) ------- COMMENT: d51e46afca090869 21 y3kI/7nq7fdurjNYNzixOWVjW1Y (comment: CHECK negative regulation of meiotic exit) ------- COMMENT: d5300c7f4c45c39f 36 b0CxYMjfw5JPcJSz2oQZZfndMnA (comment: CHECK mitotic interphase) ------- COMMENT: d5706346ead68c77 3 cobSazNkG8nDwK9d1S+b/MM6tJ8 fig 2c ------- COMMENT: d5706346ead68c77 4 cobSazNkG8nDwK9d1S+b/MM6tJ8 fig 2c ------- COMMENT: d5706346ead68c77 5 nnuWhrFU7jaQ2CsXT4A21I2oLbM fig 3a ------- COMMENT: d5706346ead68c77 6 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: d5706346ead68c77 7 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: d5706346ead68c77 19 jKE0HAk4aLf+MD8iL/svYPS8rHQ fig 8 ------- COMMENT: d5830d67c82d45cd 80 IP4i7+pqkl0K+lJqcpCUKeTc89c present throughout mitotic cell cycle ------- COMMENT: d5e1616053c30a60 2 m+Ha2jk2SQFHOz6WYMBgCaSA8OE (comment: moved down drom endocytosis.) Delayed FM4-64 uptake when in combination with a clathrin mutationSlow dynamics of endocytic patch markers ------- COMMENT: d5e1616053c30a60 6 Iwec487yfpaNBvuIhSkUlb0Drig (Fig. 6D); evidence: filipin staining ------- COMMENT: d5e1616053c30a60 7 GITHtI3IJmHBskOpdp4lUs/eHkQ (comment: moved down from abnormal protein localization to cell tip (new term)) ------- COMMENT: d5e1616053c30a60 8 TqgJ/EIetp+7zqWQqO2USpBx+Eg (Fig. 3B–D) (Fig. 4A and movies 3 and 4) slow dynamics of actin patch components: Sla1, wsc1, arc5, Crn1 ------- COMMENT: d5e1616053c30a60 16 JcWV8ynNHQVmqf23sPDPEERC+ls (comment: moved down to new term from :protein mislocalized to cytoplasm during vegetative growth) ------- COMMENT: d5e1616053c30a60 19 JcWV8ynNHQVmqf23sPDPEERC+ls (comment: moved down to new term from :protein mislocalized to cytoplasm during vegetative growth) ------- COMMENT: d5e1616053c30a60 20 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: d5e1616053c30a60 21 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: d5e1616053c30a60 22 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: d5e1616053c30a60 23 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: d5e1616053c30a60 24 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: d5e1616053c30a60 25 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: d5e1616053c30a60 26 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: d5e1616053c30a60 27 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: d5e1616053c30a60 28 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: d5e1616053c30a60 30 EG9jfXyT8Ms9D3f+RYjPjL0YaV8 fig. S2A, B ------- COMMENT: d5e1616053c30a60 32 FnoXrkDUavUxfTCif/1riD739+k Fig. S2C ------- COMMENT: d5e1616053c30a60 33 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: d5e1616053c30a60 34 RES91gMmMoAp0f8175HE7ZFD3BY fig 7b ------- COMMENT: d5e1616053c30a60 36 DKQ3+306ZcIbvQIQ6vCWLs2MBgg fig 2d ------- COMMENT: d5e1616053c30a60 37 DadqlNdFwBVy2tQgQ2txbzlcLq8 fig 2D ------- COMMENT: d5e1616053c30a60 40 VIJifWzcYy8esq6CqUEgT+B2FiI (Supporting Information Fig. S4A) ------- COMMENT: d5e1616053c30a60 41 BY2uuo1DR0/Dn+zKiec7loOOnKU (comment: FM4-64 uptake (I made Henars original annotation into a double mutant so the attribution has changed)) ------- COMMENT: d61fe0b11445626b 3 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: d61fe0b11445626b 4 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: d61fe0b11445626b 5 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: d61fe0b11445626b 6 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: d61fe0b11445626b 7 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: d61fe0b11445626b 8 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: d61fe0b11445626b 9 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: d61fe0b11445626b 10 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: d61fe0b11445626b 11 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: d61fe0b11445626b 12 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: d61fe0b11445626b 13 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: d61fe0b11445626b 14 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: d61fe0b11445626b 15 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: d61fe0b11445626b 16 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: d61fe0b11445626b 17 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: d61fe0b11445626b 20 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: d64f1fadd832246e 2 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: d64f1fadd832246e 3 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: d64f1fadd832246e 4 cCJHhVG9bMmKeWcE8qPm4dNYznE fig1 In wild-type cells, monopolar or nonpolar spindles were not observed (Fig. 4C). ------- COMMENT: d64f1fadd832246e 11 2yzHp5epI4ll3QqwtJTnDE+HITI bipolar spindle defects caused by the loss of telomere clustering were rescued by stopping nuclear movement. ------- COMMENT: d64f1fadd832246e 12 Vip4EFthsBCP9AMWlw9X0rBwtfk bipolar/spindle defects caused by the loss of telomere clustering were rescued by stopping nuclear movement. ------- COMMENT: d64f1fadd832246e 13 fx/5XTAoLn1toVrOrzWdUYANQ5o (comment: never observed 54/54) ------- COMMENT: d64f1fadd832246e 14 sU3QZqzpluF5Sh9FMS8rBZXaNdE (comment: never observed 37/37) ------- COMMENT: d64f1fadd832246e 15 zESFSthuV8uAe4keEy5ylmFcgfU Fig 6A, B even in the presence of the bipolar spindle ------- COMMENT: d64f1fadd832246e 16 jvLAsgebDGwT4mnywTPSNFIpM0A spindle defects caused by the loss of telomere clustering were rescued by stopping nuclear movement. ------- COMMENT: d6654eb8043399ed 2 kJoshqW7emo7AHAOpdALu3el1LY (comment: dependent on F-actin (asayed using Latrunculin A); independent of microtubules (assayed using MBC)) ------- COMMENT: d6654eb8043399ed 3 kJoshqW7emo7AHAOpdALu3el1LY (comment: dependent on F-actin (asayed using Latrunculin A); independent of microtubules (assayed using MBC)) ------- COMMENT: d6654eb8043399ed 5 9bEzU3Z9FtFm1BKVuhHDSebpMWY (comment: assayed using casein; doesn't quite rule out tyrosine phosphorylation) ------- COMMENT: d6654eb8043399ed 6 UN2c4xHsBpRehfx+/yhH56Yzlrs (comment: based just on this paper, candidate for involved_in_or_regulates qualifier) ------- COMMENT: d6654eb8043399ed 7 UN2c4xHsBpRehfx+/yhH56Yzlrs (comment: based just on this paper, candidate for involved_in_or_regulates qualifier) ------- COMMENT: d6654eb8043399ed 24 YxJ8oJF6kcPzaXe4NMuJV2iMI/4 (comment: CHECK same as orb3-167 alone) ------- COMMENT: d6654eb8043399ed 25 YxJ8oJF6kcPzaXe4NMuJV2iMI/4 (comment: CHECK same as orb3-167 alone) ------- COMMENT: d68133bbe06d0602 28 ZqdbKM+k6Xh+01374DU8GVCfhIo (comment: CHECK in the presence or absence of Nbs1) ------- COMMENT: d68133bbe06d0602 32 9J2smoKjBAXkcv+Pc0iRxqk4DNs (comment: CHECK in absence of Nbs1) ------- COMMENT: d68133bbe06d0602 33 9J2smoKjBAXkcv+Pc0iRxqk4DNs (comment: CHECK in absence of Nbs1) ------- COMMENT: d68133bbe06d0602 81 gQTor2PcxNfzDBJpmp+N7KB6yV0 (comment: localization of mutated protein assayed) ------- COMMENT: d68db45202ee441d 20 d8bijJl7vrKO9mbbsXCk1mFCBN0 evidence for all FYPO:0001908 = northern blot ------- COMMENT: d6a49443005b8f21 5 9IwGQj3fqdzGKzuUUiNBi6DiNDs e pyruvate kinase, thiazole biosynthetic enzyme, and ribosomal protein L25-A ------- COMMENT: d6a49443005b8f21 6 9nkIVxeWoiLrZb1mBGug941nqaU These data indicate that the decrease in ribosomal protein L25-A observed in cpc2::ura4 cells is probably due to a defect in recruitment of its mRNA to polyribosomes. The decreased amounts of both sam1 and thi2 RNAs are sufficient to account for the lowered protein abundance of each in cpc2::ura4 cells. In contrast, the decline in the level of ribosomal protein Rpl25 in cpc2::ura4 cells is not likely to be caused by an inability to accumulate its mRNA transcript. ------- COMMENT: d6a88cd70209aaf6 2 gQh6KtvOAP8Es8j/21OzWWDx8Dg transcriptional activity was dramatically increased in these 11 mitochondrial mutant strains (Figure 1i,j). ------- COMMENT: d6a88cd70209aaf6 4 2Mh+l23pAAXV5chHdqtxiV9UIPQ Figure 1m ------- COMMENT: d6a88cd70209aaf6 5 2Mh+l23pAAXV5chHdqtxiV9UIPQ Figure 1m ------- COMMENT: d6a88cd70209aaf6 6 MXUnyVedP3SARYb1ogW8BPlhcO0 To further examine whether ROS and NO mediated increased Rst2 transcriptional activity caused by mitochondrial complex III/IV inhibitors, we examined the effect of antioxidant N-acetyl-L-cysteine (NAC) which aids in ROS detoxification and 2-(4-Carboxyphenyl)-4,4,5,5-tetramethy-limidazo-line-1-oxyl-3- oxide (Carboxy-PTIO), a NO-specific scavenger on the Rst2 transcriptional activity stimulated by mitochondrial complex III/IV inhibitors. The results showed that NAC and Carboxy-PTIO significantly inhibited mitochondrial complex III/IV inhibitors-induced activation of Rst2 in a dose-dependent manner (Figure 2e–j), suggesting that ROS and NO were involved in mitochondrial respiratory chain complex III/IV inhibitors-induced activation of Rst2. To further prove this result, we also examined the effect of a mammalian NO synthesis (NOS) inhibitor, N G-nitro-l-arginine methyl ester (NAME) on the Rst2 transcriptional activity stimulated by mitochondrial complex III/IV inhibitors, since it was reported that NAME treatment can reduce NO formation by more than 60% in yeast cells (Astuti et al., 2016a). As expected, NAME dose-dependently decreased mitochondrial complex III/IV inhibitors- induced Rst2 activation (Figure 2k–m). ------- COMMENT: d6a88cd70209aaf6 7 ZVK4zltwcUwYn6ylynDcSW0YxAI (Figure 2k–m). ------- COMMENT: d6a88cd70209aaf6 8 MNtLEpDRYv1C2tqG34qluorPEyE Figure 2n ------- COMMENT: d6a88cd70209aaf6 9 MNtLEpDRYv1C2tqG34qluorPEyE Figure 2n ------- COMMENT: d6a88cd70209aaf6 10 MNtLEpDRYv1C2tqG34qluorPEyE Figure 2n ------- COMMENT: d6a88cd70209aaf6 11 2HeWMjHvW/V3Yk2oj0ob72OwMmY Fig. 3a glucose r excess ------- COMMENT: d6a88cd70209aaf6 12 dTGwj2mXZhcDYHKWsysc3tru0+w Fig. 3a glucose starve ------- COMMENT: d6a88cd70209aaf6 13 dTGwj2mXZhcDYHKWsysc3tru0+w Fig. 3a glucose starve ------- COMMENT: d6a88cd70209aaf6 26 MTXk0NRdJL2XfZcRFvZcLcLg3ds figure 4b ------- COMMENT: d6a88cd70209aaf6 27 MTXk0NRdJL2XfZcRFvZcLcLg3ds figure 4b ------- COMMENT: d6a88cd70209aaf6 28 MTXk0NRdJL2XfZcRFvZcLcLg3ds figure 4b ------- COMMENT: d6a88cd70209aaf6 29 MTXk0NRdJL2XfZcRFvZcLcLg3ds figure 4b ------- COMMENT: d6a88cd70209aaf6 30 MTXk0NRdJL2XfZcRFvZcLcLg3ds figure 4b ------- COMMENT: d6a88cd70209aaf6 31 MTXk0NRdJL2XfZcRFvZcLcLg3ds figure 4b ------- COMMENT: d6a88cd70209aaf6 32 MTXk0NRdJL2XfZcRFvZcLcLg3ds figure 4b ------- COMMENT: d6a88cd70209aaf6 33 MTXk0NRdJL2XfZcRFvZcLcLg3ds figure 4b ------- COMMENT: d6a88cd70209aaf6 34 MTXk0NRdJL2XfZcRFvZcLcLg3ds figure 4b ------- COMMENT: d6a88cd70209aaf6 35 MTXk0NRdJL2XfZcRFvZcLcLg3ds figure 4b ------- COMMENT: d6a88cd70209aaf6 36 MTXk0NRdJL2XfZcRFvZcLcLg3ds figure 4b ------- COMMENT: d6a88cd70209aaf6 37 MTXk0NRdJL2XfZcRFvZcLcLg3ds figure 4b ------- COMMENT: d6a88cd70209aaf6 38 hI5t2isRnTMyTI6yWT985lhWG+s Table1 ------- COMMENT: d6a88cd70209aaf6 39 gQh6KtvOAP8Es8j/21OzWWDx8Dg transcriptional activity was dramatically increased in these 11 mitochondrial mutant strains (Figure 1i,j). ------- COMMENT: d6a88cd70209aaf6 40 gQh6KtvOAP8Es8j/21OzWWDx8Dg transcriptional activity was dramatically increased in these 11 mitochondrial mutant strains (Figure 1i,j). ------- COMMENT: d6a88cd70209aaf6 41 gQh6KtvOAP8Es8j/21OzWWDx8Dg transcriptional activity was dramatically increased in these 11 mitochondrial mutant strains (Figure 1i,j). ------- COMMENT: d6a88cd70209aaf6 42 gQh6KtvOAP8Es8j/21OzWWDx8Dg transcriptional activity was dramatically increased in these 11 mitochondrial mutant strains (Figure 1i,j). ------- COMMENT: d6a88cd70209aaf6 43 gQh6KtvOAP8Es8j/21OzWWDx8Dg transcriptional activity was dramatically increased in these 11 mitochondrial mutant strains (Figure 1i,j). ------- COMMENT: d6a88cd70209aaf6 44 gQh6KtvOAP8Es8j/21OzWWDx8Dg transcriptional activity was dramatically increased in these 11 mitochondrial mutant strains (Figure 1i,j). ------- COMMENT: d6a88cd70209aaf6 45 gQh6KtvOAP8Es8j/21OzWWDx8Dg transcriptional activity was dramatically increased in these 11 mitochondrial mutant strains (Figure 1i,j). ------- COMMENT: d6a88cd70209aaf6 46 gQh6KtvOAP8Es8j/21OzWWDx8Dg transcriptional activity was dramatically increased in these 11 mitochondrial mutant strains (Figure 1i,j). ------- COMMENT: d6a88cd70209aaf6 47 gQh6KtvOAP8Es8j/21OzWWDx8Dg transcriptional activity was dramatically increased in these 11 mitochondrial mutant strains (Figure 1i,j). ------- COMMENT: d6a88cd70209aaf6 48 gQh6KtvOAP8Es8j/21OzWWDx8Dg transcriptional activity was dramatically increased in these 11 mitochondrial mutant strains (Figure 1i,j). ------- COMMENT: d6a88cd70209aaf6 50 hI5t2isRnTMyTI6yWT985lhWG+s Table1 ------- COMMENT: d6a88cd70209aaf6 51 hI5t2isRnTMyTI6yWT985lhWG+s Table1 ------- COMMENT: d6e965719f1ee102 1 uG37Tw7s31YUWcUeYylvsqvYHdw (comment: alpha-1,2-galactosylation of O-linked glycan) ------- COMMENT: d6e965719f1ee102 2 JP70/oo4M8KEKgUeFM6nq97bn7Q (comment: alpha-1,2-galactosylation of N-linked glycan) ------- COMMENT: d6e965719f1ee102 3 JP70/oo4M8KEKgUeFM6nq97bn7Q (comment: alpha-1,2-galactosylation of N-linked glycan) ------- COMMENT: d6e965719f1ee102 4 JP70/oo4M8KEKgUeFM6nq97bn7Q (comment: alpha-1,2-galactosylation of N-linked glycan) ------- COMMENT: d6e965719f1ee102 5 uG37Tw7s31YUWcUeYylvsqvYHdw (comment: alpha-1,2-galactosylation of O-linked glycan) ------- COMMENT: d6e965719f1ee102 6 P2F9cMHWIe4LfWG7m8d9CoDXTKE (comment: alpha-1,3-galactosylation of O-linked glycan) ------- COMMENT: d6e965719f1ee102 7 P2F9cMHWIe4LfWG7m8d9CoDXTKE (comment: alpha-1,3-galactosylation of O-linked glycan) ------- COMMENT: d6e965719f1ee102 8 P2F9cMHWIe4LfWG7m8d9CoDXTKE (comment: alpha-1,3-galactosylation of O-linked glycan) ------- COMMENT: d6f2102e260ba45e 5 ZEh0tgkZv/+Fgw34jxoRTRCMO3s (comment: CHECK actually this only occurs in 30% of cells.. I don't know if it is viable or inviable) ------- COMMENT: d6f2102e260ba45e 7 tK+02Vn/vqK4TzVaY0KmzX0Kvv0 (comment: CHECK parent child relationship with term above requested) ------- COMMENT: d6f35a8bb66ac367 1 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: d6f35a8bb66ac367 2 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: d6f35a8bb66ac367 3 PZmTqVNICZEpeq/HdDYMkrCdKYE Examination by HPLC of the nucleoside composition of purified tRNATyr(GUA) from trm6Δ and trm61Δ mutants revealed that m1A levels were less than 0.03 moles/mole, compared to 0.60 moles/mole in WT cells, whereas levels of C, m5C, and m7G were very similar in the tRNATyr(GUA) from both mutant and WT cells (Fig 1B and 1C) ------- COMMENT: d6f35a8bb66ac367 4 PZmTqVNICZEpeq/HdDYMkrCdKYE Examination by HPLC of the nucleoside composition of purified tRNATyr(GUA) from trm6Δ and trm61Δ mutants revealed that m1A levels were less than 0.03 moles/mole, compared to 0.60 moles/mole in WT cells, whereas levels of C, m5C, and m7G were very similar in the tRNATyr(GUA) from both mutant and WT cells (Fig 1B and 1C) ------- COMMENT: d6f35a8bb66ac367 5 CWz6GuGF0W/2koXxozO+8Tv2q3Q Similarly, poison primer extension showed that tRNAiMet(CAU) was nearly completely modified with m1A58 in WT cells (97%), but not visibly modified in trm6Δ and trm61Δ mutants (although quantifica- tion with the high background gave 2.0% for trm6Δ and 2.8% in trm61Δ). ------- COMMENT: d6f35a8bb66ac367 6 CWz6GuGF0W/2koXxozO+8Tv2q3Q Similarly, poison primer extension showed that tRNAiMet(CAU) was nearly completely modified with m1A58 in WT cells (97%), but not visibly modified in trm6Δ and trm61Δ mutants (although quantifica- tion with the high background gave 2.0% for trm6Δ and 2.8% in trm61Δ). ------- COMMENT: d6f35a8bb66ac367 7 lTJDrJ+pFxigCngRAd8GOfROCRU These results show that S. pombe trm6+ and trm61+ are required for all detectable m1A58 modification of cytoplasmic tRNAs. ------- COMMENT: d6f35a8bb66ac367 8 lTJDrJ+pFxigCngRAd8GOfROCRU These results show that S. pombe trm6+ and trm61+ are required for all detectable m1A58 modification of cytoplasmic tRNAs. ------- COMMENT: d6f35a8bb66ac367 11 5k6au32Okx/fyie+WcdO1HliWZ0 The northern analysis revealed that tRNAiMet(CAU) levels were substantially reduced in the S. pombe trm6Δ mutants, both at 30 ̊C and 38.5 ̊C. At 30 ̊C, tRNAiMet(CAU) levels were 49% of those in WT cells, whereas each of the other eight tRNAs had levels between 82% and 121% of those in WT cells (Figs 2A, 2B and S3). ------- COMMENT: d6f35a8bb66ac367 12 yRwZf9XXTJ/qTwioUk6mUNMJFdE Similarly, the temperature sensitivity of the S. pombe trm61Δ strain was completely suppressed by expression of the stand-alone imt06+ gene (S5 Fig). ------- COMMENT: d6f35a8bb66ac367 13 vv/RlTgntJEkY7+QLB/OajbeS+g We found that the temperature sensitive growth defect of S. pombe trm6Δ mutants on EMMC-leu media was completely suppressed by expression of the stand-alone imt06+ gene, growing identically to that of an S. pombe trm6Δ [Ptrm6 trm6+] strain at high temperature (Fig 2C), ------- COMMENT: d6f35a8bb66ac367 14 DWnCgYtrTUwp1ni9Jqq76Mi/l+A Growth analysis on plates showed that the trm6Δ dhp1-5 and trm6Δ dhp1-6 mutants were nearly as healthy at high temperatures as the WT strain on both YES and EMMC-his media, whereas the trm6Δ tol1-1 mutant was slightly less healthy at higher temperatures (Fig 3A). ------- COMMENT: d6f35a8bb66ac367 15 DWnCgYtrTUwp1ni9Jqq76Mi/l+A Growth analysis on plates showed that the trm6Δ dhp1-5 and trm6Δ dhp1-6 mutants were nearly as healthy at high temperatures as the WT strain on both YES and EMMC-his media, whereas the trm6Δ tol1-1 mutant was slightly less healthy at higher temperatures (Fig 3A). ------- COMMENT: d6f35a8bb66ac367 16 VrbRhn48Q///nJAKkenjCGFq+9U Northern analysis of tRNA from strains grown at 30 ̊C and after temperature shift to 38.5 ̊C showed that the dhp1 and tol1 suppressors substantially restored tRNAiMet(CAU) levels at both high and low temperatures, without affecting any of a number of other tRNAs (Fig 3B and 3C). ------- COMMENT: d6f35a8bb66ac367 18 +TZYTsvyjQHJcZpwM+5O2D7YfYo The discovery of dhp1 and tol1 mutations as suppressors of the S. pombe trm6Δ temperature sensitivity demonstrates the involvement of the RTD pathway in decay of tRNAiMet(CAU) lack- ing m1A58 in S. pombe. ------- COMMENT: d6f35a8bb66ac367 19 +TZYTsvyjQHJcZpwM+5O2D7YfYo The discovery of dhp1 and tol1 mutations as suppressors of the S. pombe trm6Δ temperature sensitivity demonstrates the involvement of the RTD pathway in decay of tRNAiMet(CAU) lack- ing m1A58 in S. pombe. ------- COMMENT: d6f35a8bb66ac367 21 yRwZf9XXTJ/qTwioUk6mUNMJFdE Similarly, the temperature sensitivity of the S. pombe trm61Δ strain was completely suppressed by expression of the stand-alone imt06+ gene (S5 Fig). ------- COMMENT: d6f35a8bb66ac367 22 8YdN7mK/q85W4SFom4Ne7xCRBPs As anticipated, the resulting trm6Δ imt06Δ strain grew very poorly at 30 ̊C, and was temperature sensitive at higher tem- peratures, not growing at all at 37 ̊C, ------- COMMENT: d6f35a8bb66ac367 23 JxWNmIJPTGLxgg1KDkUfkSCycMA As anticipated, the resulting trm6Δ imt06Δ strain grew very poorly at 30 ̊C, and was temperature sensitive at higher tem- peratures, not growing at all at 37 ̊C, These results show a prominent synthetic growth defect in the S. pombe trm6Δ imt06Δ strain, due only to reduced levels of tRNAiMet(CAU). ------- COMMENT: d6f35a8bb66ac367 24 kH3YACnuA8jTKrkhV1X31ApPmPE We observed little, if any, suppression of the trm6Δ growth defect in the trm6Δ cid14Δ strains (S13A Fig), and only very minor restoration of tRNAiMet(CAU) levels at high tempera- ture, relative to levels in trm6Δ mutants (21% vs 18%, compared to 39% in the trm6Δ dhp1-5 strain) (S13B and S13C Fig). Thus, we infer that tRNAiMet(CAU) is degraded in S. pombe trm6Δ and trm6Δ imt06Δ mutants primarily by the RTD pathway, and not appreciably by the TRAMP complex of the nuclear surveillance pathway. ------- COMMENT: d6f35a8bb66ac367 25 VrbRhn48Q///nJAKkenjCGFq+9U Northern analysis of tRNA from strains grown at 30 ̊C and after temperature shift to 38.5 ̊C showed that the dhp1 and tol1 suppressors substantially restored tRNAiMet(CAU) levels at both high and low temperatures, without affecting any of a number of other tRNAs (Fig 3B and 3C). ------- COMMENT: d712ca99b02b1957 1 9ndchKkkbp+dpP1QfK1Qt2z4Lw4 One of the diploid clones (C1) was allowed to sporulate and ;100 of the haploids obtained were tested for resistance to G418. We found no resis- tants, which implicates that spSNW1 is an essential gene in S. pombe. ------- COMMENT: d735a52f09029ebf 1 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: d735a52f09029ebf 3 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: d735a52f09029ebf 4 5zLBcPfE96G7rFQPJBZsaEOa1Og Fig 4C ------- COMMENT: d735a52f09029ebf 5 dyYw6ZalfdIoxY5DSVEiJgi6MUA Fig S2A We first validated the previous report of cbf11Δ cells being sensitive to TBZ. When plated on YES medium containing TBZ, the cbf11Δ mutant indeed showed strong sensitivity to the drug (Fig. S2A), ------- COMMENT: d735a52f09029ebf 6 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: d735a52f09029ebf 8 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: d735a52f09029ebf 10 ogA6CX0mAXl6Nuw5dAI8MjfxMPo Fig 4A, S3 ------- COMMENT: d735a52f09029ebf 11 oK9gS934BpczjXnrFrhg0qjS6wQ Fig 4A, S2 ------- COMMENT: d735a52f09029ebf 14 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: d735a52f09029ebf 15 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: d735a52f09029ebf 17 2tpJ2pdSU1k53FeiX74cgkWhvbE Fig 2G ------- COMMENT: d735a52f09029ebf 18 hobAwDDkcGv8lsWinHMoy3Abxoo Fig 2E ------- COMMENT: d735a52f09029ebf 19 urOpmJ7V2cOTeJDX6yi2ZCNyVFA Fig 4D ------- COMMENT: d735a52f09029ebf 23 6QWpsa+ewsdvpHOMgyngJRsVf7g Notably, the nuclear displacement phenotypewas associated with mid-anaphase spindle bending and/or detachment of one of the daughter chromosome masses from the spindle, and subsequent spindle disassembly and merger of the two daughter chromosome masses into one diploid nucleus (Figs 1B and 2B). ------- COMMENT: d77be516bab87443 1 rw3s1R/Tl7ZGqRRm413Qz1VKJsQ (Fig 3C and D). Indeed, the distribution of the GFP dots revealed aberrant segregation of sister chromatids in MI of clr4F449Y/clr4F449Y cells, with a high frequency of equational segregation that normally occurs during MII (Fig 3D ------- COMMENT: d77be516bab87443 3 scPVZSfO321X3AbYRcqd/PYk2as clr4F449Y/clr4F449Y cells displayed strongly ele- vated H3K9me2 levels when in mitosis, while H3K9me3 was absent (Figs 2A and EV3A). ------- COMMENT: d77be516bab87443 4 scPVZSfO321X3AbYRcqd/PYk2as clr4F449Y/clr4F449Y cells displayed strongly ele- vated H3K9me2 levels when in mitosis, while H3K9me3 was absent (Figs 2A and EV3A). ------- COMMENT: d77be516bab87443 5 V/zzLpgXf5Bjxg1+USU3yD6H/xU (comment: CHECK Check tomorrow should this be tri methylation) ------- COMMENT: d77be516bab87443 7 rw3s1R/Tl7ZGqRRm413Qz1VKJsQ (Fig 3C and D). Indeed, the distribution of the GFP dots revealed aberrant segregation of sister chromatids in MI of clr4F449Y/clr4F449Y cells, with a high frequency of equational segregation that normally occurs during MII (Fig 3D ------- COMMENT: d77be516bab87443 8 y/ZkHvYv4eTw57/FOJ5KdWFjRYE Consistent with earlier findings, we frequently observed spores with two GFP dots in a sin- gle nucleus of a tetrad derived from clr4D/clr4D cells (Fig 3E, green fraction). Such a pattern (i.e., normal segregation during MI but not MII) occurred in < 1% of clr4F449Y/clr4F449Y cells that we have ana- lyzed (Fig 3E) ------- COMMENT: d77be516bab87443 9 m9PuQZMN4JgcGJV1KB/6bsVsuIQ figure 3F ------- COMMENT: d77be516bab87443 10 UCtIUGrT9m9gm1WmjxyA3wHnAoI figure 4a ------- COMMENT: d77be516bab87443 11 UCtIUGrT9m9gm1WmjxyA3wHnAoI figure 4a ------- COMMENT: d77be516bab87443 12 FCYYnfKHuqCdjC7ueZs3sgqz50M Figure 4A (This obser- vation indicates that highest binding affinity of Swi6 towards H3 is necessary for proper subnuclear localization in meiocytes, for which H3K9 needs to be tri-methylated. ------- COMMENT: d77be516bab87443 13 5/84cv80Nw3civ/QtkQ00JbZa3c Furthermore, fewer clr4F449Y/clr4F449Y cells displayed lagging DNA upon expression of Swi6Chp1-like-CD (Fig 4C and D). ------- COMMENT: d77be516bab87443 14 qmsg+SlHyKujbXb1jiSnmFkSwKE spores formed colonies again (Fig 4G). ------- COMMENT: d77be516bab87443 17 1Utqfc7yL51qLU0Mowdn6fsbUAY Upon inhibition of Cdk1-as, Clr4S458 phosphorylation rapidly decreased and was undetectable 3 h postinhibition, whereas Clr4 phosphorylation levels remained unaffected in cdk1+ cells (Figs 6D and EV5D). ------- COMMENT: d77be516bab87443 18 KOHFOETV+9EX0nv9igPLjSEoHrc Indeed, CDK1/Cyclin B phosphorylated recombinant Clr4 specifically at S458 (Fig 6E). ------- COMMENT: d77be516bab87443 20 7W/KfXsgK/Y04SKDt/nh99iYMgg This revealed reduced H3K9me2, but increased H3K9me3 levels upon 1-NM-PP1 addition in cdk1-as cells specifi- cally (Fig 6F), ------- COMMENT: d77be516bab87443 21 scPVZSfO321X3AbYRcqd/PYk2as clr4F449Y/clr4F449Y cells displayed strongly ele- vated H3K9me2 levels when in mitosis, while H3K9me3 was absent (Figs 2A and EV3A). ------- COMMENT: d77c8e04bc2a8fba 1 e+EAbdrFWp0pJ4c+2eZgRHeYMgY (comment: vw: added because I saw in an intro of another [paper) ------- COMMENT: d7f404ebd5804111 1 27wD8l6ipbmXSYcdGFjhpwlwSwE (comment: dependent on actin cytoskeleton (assayed using Latrunculin A)) ------- COMMENT: d7f404ebd5804111 2 27wD8l6ipbmXSYcdGFjhpwlwSwE (comment: dependent on actin cytoskeleton (assayed using Latrunculin A)) ------- COMMENT: d7f404ebd5804111 5 yqliUHeJvGzeVLODWs//8wgAo/E in vitro bundling, detected by microscopy; Figure 2 ------- COMMENT: d7f404ebd5804111 6 xE+kiwZF4aXrZPkk+Sy1dMRlR50 (comment: assayed using purified rabbit skeletal muscle F-actin) ------- COMMENT: d7f404ebd5804111 52 1MVRGKpGIPuqC6NJ0bf9VxJX3oM (comment: temperature permissive for cdc4-8) ------- COMMENT: d7f404ebd5804111 53 Cws+vM/IABfVq7Ho44QSsB323jg (comment: temperature restrictive for cdc4-8) ------- COMMENT: d7f404ebd5804111 61 vObu/oGSQA0DEk8XapCpNjUIxw4 (comment: various abnormal shapes) ------- COMMENT: d7f404ebd5804111 67 vObu/oGSQA0DEk8XapCpNjUIxw4 (comment: various abnormal shapes) ------- COMMENT: d7f404ebd5804111 72 jV2NOcbTpDgD7VFvtc2w38dlAiE (comment: CONDITION 30 degrees C) ------- COMMENT: d7f404ebd5804111 73 AYCjehAu9sPE8NAVGuF7gexrZ5A (comment: CONDITION 27 degrees C) ------- COMMENT: d7f404ebd5804111 74 AYCjehAu9sPE8NAVGuF7gexrZ5A (comment: CONDITION 27 degrees C) ------- COMMENT: d7f404ebd5804111 75 jV2NOcbTpDgD7VFvtc2w38dlAiE (comment: CONDITION 30 degrees C) ------- COMMENT: d7f404ebd5804111 76 AYCjehAu9sPE8NAVGuF7gexrZ5A (comment: CONDITION 27 degrees C) ------- COMMENT: d800c02f9eed68d9 3 +uh4Tmv2WPwn+YWz/5dhC1YZnj4 Sdj1, carrying a C-terminal YFP, FLAG, and His6 (YFH) tag, was evenly distributed throughout the cytosol and nucleus, and did not markedly co-localize with the mitochondrial marker protein, Cox4 (Fig. 2A). ------- COMMENT: d800c02f9eed68d9 27 +uh4Tmv2WPwn+YWz/5dhC1YZnj4 Sdj1, carrying a C-terminal YFP, FLAG, and His6 (YFH) tag, was evenly distributed throughout the cytosol and nucleus, and did not markedly co-localize with the mitochondrial marker protein, Cox4 (Fig. 2A). ------- COMMENT: d800c02f9eed68d9 28 nw5FwOysn4MPbNbLH7lNIOBRwRo As expected, we found that GST-tagged Sdj1 was able to co-precipitate wild type His6- tagged Sdj1, but not His6-tagged Sdj1-L169P (Fig. 1B). In agree- ment with this, we observe that the Leu-169 residue is located in the proximity of the subunit-subunit interface in the published crystal structure of Sdj1 (33) (Fig. 1C). ------- COMMENT: d800c02f9eed68d9 29 0cqMeSyAKEYqKQf2rgJcA0iV7oc First, we observed that the steady-state level of Sdj1-L169P was reduced compared with wild type Sdj1 (Fig. 3A). In cultures where protein synthesis was blocked by addition of cyclohexi- mide, we found that wild type Sdj1 appeared stable, whereas the Sdj1-L169P protein was rapidly degraded (Fig. 3B). However, when the proteasome inhibitor bortezomib was added to the culture, the degradation was blocked (Fig. 3B), suggesting that the reduced Sdj1-L169P steady-state level was primarily caused by proteasomal degradation of the protein ------- COMMENT: d800c02f9eed68d9 32 kE0Xf5jsbeH3AYtXfZT/vs0liDE Interestingly, a few proteins were found to specifically inter- act with Sdj1-L169P (supplemental File S2). Among these were Ssa1 and Ssa2, two highly similar cytosolic Hsp70-type chaper- ones. To independently validate the data from mass spectrom- etry, we subsequently performed immunoprecipitation experi- ments with wild type Sdj1 and Sdj1-L169P. Indeed, we found that Hsp70 (Ssa1 and Ssa2) was specifically associated with Sdj1-L169P and not wild type Sdj1 (Fig. 4A). ------- COMMENT: d800c02f9eed68d9 34 95WJNioXE+MtAclCv9cD6wv0HJI . Indeed, the Sdj1-L169P level was increased in ssa1, ssa2, and hsp104 null mutants, with the most marked effect observed in the ssa2 and hsp104 strains (Fig. 4B). ------- COMMENT: d800c02f9eed68d9 35 95WJNioXE+MtAclCv9cD6wv0HJI . Indeed, the Sdj1-L169P level was increased in ssa1, ssa2, and hsp104 null mutants, with the most marked effect observed in the ssa2 and hsp104 strains (Fig. 4B). ------- COMMENT: d800c02f9eed68d9 36 95WJNioXE+MtAclCv9cD6wv0HJI . Indeed, the Sdj1-L169P level was increased in ssa1, ssa2, and hsp104 null mutants, with the most marked effect observed in the ssa2 and hsp104 strains (Fig. 4B). ------- COMMENT: d800c02f9eed68d9 37 IWBngsZeRMrHr7uQl32Lb4IUDnY (comment: =) ------- COMMENT: d800c02f9eed68d9 38 kzOwVg3e7v5zFmh4C7rp1MTleP4 only in the ltn1 strain did we observe an increased level of Sdj1-L169P (Fig. 5A). ------- COMMENT: d800c02f9eed68d9 40 se6iUbhZRAKG0Vi02fgSCP7O42E Indeed, Sdj1-L169P was more stable in both ltn1 and rqc1 deletion strains (Fig. 5B). Although degradation was still observed in the ltn1 and rqc1 strains, Sdj1-L169P was signif- icantly stabilized in these mutants compared with the wild type control (Fig. 5C). ------- COMMENT: d800c02f9eed68d9 46 Hhi4lHEm4Sz0UdjiTwNsRM/gKKc (comment: vw: this pathway appears to be proteasome independent, likely autophagy mediated) ------- COMMENT: d800c02f9eed68d9 47 jvMl2xOZGy0ZSEDYDWduaIUpaLA (comment: vw: this pathway appears to be proteasome independent, likely autohagy mediated) ------- COMMENT: d81e6d582d5b3b8f 1 HovSav234O3Vbu4sJ1EBzakFF8s A spd1 deletion partially suppresses the synthetic growth defect in a brc1 ddb1 double mutant background ------- COMMENT: d81e6d582d5b3b8f 7 pwSbRtesv5qKbvfe48nH0Io9EhE A spd1 deletion partially suppresses the synthetic growth defect in a brc1 csn1 double mutant background ------- COMMENT: d81e6d582d5b3b8f 43 pwSbRtesv5qKbvfe48nH0Io9EhE A spd1 deletion partially suppresses the synthetic growth defect in a brc1 csn1 double mutant background ------- COMMENT: d81e6d582d5b3b8f 44 HovSav234O3Vbu4sJ1EBzakFF8s A spd1 deletion partially suppresses the synthetic growth defect in a brc1 ddb1 double mutant background ------- COMMENT: d81e6d582d5b3b8f 54 XG2xrqfHMgYDsdoaMzbunWVw2xQ spd1 deletion suppresses brc1delta ddb1delta sensitivity to DNA damage agents ------- COMMENT: d81e6d582d5b3b8f 58 7ocykKo6t5O26VH9XjV00MClzsQ spd1 deletion suppresses brc1delta csn1delta sensitivity to DNA damage agents ------- COMMENT: d81e6d582d5b3b8f 203 XG2xrqfHMgYDsdoaMzbunWVw2xQ spd1 deletion suppresses brc1delta ddb1delta sensitivity to DNA damage agents ------- COMMENT: d81e6d582d5b3b8f 208 7ocykKo6t5O26VH9XjV00MClzsQ spd1 deletion suppresses brc1delta csn1delta sensitivity to DNA damage agents ------- COMMENT: d81e6d582d5b3b8f 209 7ocykKo6t5O26VH9XjV00MClzsQ spd1 deletion suppresses brc1delta csn1delta sensitivity to DNA damage agents ------- COMMENT: d81e6d582d5b3b8f 210 7ocykKo6t5O26VH9XjV00MClzsQ spd1 deletion suppresses brc1delta csn1delta sensitivity to DNA damage agents ------- COMMENT: d81e6d582d5b3b8f 211 XG2xrqfHMgYDsdoaMzbunWVw2xQ spd1 deletion suppresses brc1delta ddb1delta sensitivity to DNA damage agents ------- COMMENT: d81e6d582d5b3b8f 212 XG2xrqfHMgYDsdoaMzbunWVw2xQ spd1 deletion suppresses brc1delta ddb1delta sensitivity to DNA damage agents ------- COMMENT: d84353df94449c44 2 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: d84353df94449c44 3 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: d84353df94449c44 4 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: d84353df94449c44 5 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: d84353df94449c44 6 Mf87d+JaK4YguHC6nZu1eLfH3Ck fig 7C ------- COMMENT: d84353df94449c44 7 Mf87d+JaK4YguHC6nZu1eLfH3Ck fig 7C ------- COMMENT: d84353df94449c44 8 Mf87d+JaK4YguHC6nZu1eLfH3Ck fig 7C ------- COMMENT: d84353df94449c44 9 cKQ8OBeqWXljxOQzyOlNDdSqv/E fig 7C (comment: CHECK supression of trm7-delta) ------- COMMENT: d84353df94449c44 10 Mf87d+JaK4YguHC6nZu1eLfH3Ck fig 7C ------- COMMENT: d84353df94449c44 11 cKQ8OBeqWXljxOQzyOlNDdSqv/E fig 7C (comment: CHECK supression of trm7-delta) ------- COMMENT: d84353df94449c44 12 cKQ8OBeqWXljxOQzyOlNDdSqv/E fig 7C (comment: CHECK supression of trm7-delta) ------- COMMENT: d84353df94449c44 13 cKQ8OBeqWXljxOQzyOlNDdSqv/E fig 7C (comment: CHECK supression of trm7-delta) ------- COMMENT: d84353df94449c44 14 cKQ8OBeqWXljxOQzyOlNDdSqv/E fig 7C (comment: CHECK supression of trm7-delta) ------- COMMENT: d84353df94449c44 15 cKQ8OBeqWXljxOQzyOlNDdSqv/E fig 7C (comment: CHECK supression of trm7-delta) ------- COMMENT: d8565d86bd6dba28 1 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: d8565d86bd6dba28 2 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: d8565d86bd6dba28 3 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: d8565d86bd6dba28 4 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: d8565d86bd6dba28 5 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: d8565d86bd6dba28 6 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: d8565d86bd6dba28 7 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: d8565d86bd6dba28 8 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: d8565d86bd6dba28 9 7cOUWlY6dB5W4TN6Gwmp9LFjrCw (comment: CHECK Supplement) ------- COMMENT: d8565d86bd6dba28 11 U/PvCZ3/sYCTc+VkXd+IsT2uQcI fig1b ------- COMMENT: d8565d86bd6dba28 12 U/PvCZ3/sYCTc+VkXd+IsT2uQcI fig1b ------- COMMENT: d8565d86bd6dba28 13 U/PvCZ3/sYCTc+VkXd+IsT2uQcI fig1b ------- COMMENT: d8565d86bd6dba28 14 U/PvCZ3/sYCTc+VkXd+IsT2uQcI fig1b ------- COMMENT: d8565d86bd6dba28 15 U/PvCZ3/sYCTc+VkXd+IsT2uQcI fig1b ------- COMMENT: d86816507d979037 4 9bbFArRgBvVsWJv+LeFEaimWphg Figure S1D ------- COMMENT: d86816507d979037 8 GYIiGOUF2v7YXQ4JDAO5DVx2dXU (comment: CHECK V348A) ------- COMMENT: d86816507d979037 12 d2oxFhzrzxJun+3AkAZHjg4tqdU (Figure S7A), ------- COMMENT: d86816507d979037 13 1ndnZQ2P0yRvKtTgpVuKSqkPUdY (Figure S7A) ------- COMMENT: d86816507d979037 14 O6Up2Do5XDs/HGr68EPZ+CsSX/0 (comment: weird!) Intriguingly, the double nda2noD nda3noD mutant was less sensitive than the single ones. Therefore, a possible explanation for the lower sensitivity of the double mutant is that defects resulting from the elevated level of one monomer are compensated for by a similar overexpression of the second monomer. ------- COMMENT: d86816507d979037 15 xfr35gLLq+m6t9BL1Tua/2vFVPM Both mutants displayed meiosis defects with a reduction in gamete numbers and viability more marked in the nda2noD mutant (Figures 6A, 6B, and 6C). ------- COMMENT: d86816507d979037 16 0Vb5Pcwefa8CwP24VtOBIDlvefo Figures 6I, 6J ------- COMMENT: d86816507d979037 28 VuroDWbBJv2HvysArN+Vhz4b5Go Figure 6E When dus3D diploids were induced to enter meiosis, the time needed in prophase for spindle assembly in MI was increased, while a marked in- crease in the duration of metaphase was noted in both MI and MII (Figures 6D–6H). ------- COMMENT: d89a7babeb290b75 1 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: d89a7babeb290b75 2 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: d89a7babeb290b75 3 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: d89a7babeb290b75 4 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: d89a7babeb290b75 5 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: d89a7babeb290b75 6 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 7 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 8 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 9 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 10 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 11 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 12 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 13 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 14 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 15 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 16 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 17 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 18 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 19 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 20 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 21 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 22 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 23 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 24 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 25 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 26 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 27 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 28 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 29 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 30 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 31 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 32 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 33 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 34 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 35 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 36 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 37 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 38 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 39 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 40 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 41 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 42 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 43 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 44 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 45 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 46 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 47 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 48 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 49 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 50 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 51 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 52 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 53 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 54 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 55 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 56 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 57 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 58 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 59 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 60 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 61 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 62 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 63 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 64 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: d89a7babeb290b75 65 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: d89a7babeb290b75 66 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: d89a7babeb290b75 67 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: d89a7babeb290b75 68 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: d89a7babeb290b75 69 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: d89a7babeb290b75 70 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: d89a7babeb290b75 71 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: d89a7babeb290b75 72 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: d89a7babeb290b75 73 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: d89a7babeb290b75 74 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: d89a7babeb290b75 75 Xb1L1D0oeozHWjRnmVjhdBSDo98 Fig. S3A ------- COMMENT: d89a7babeb290b75 76 Xb1L1D0oeozHWjRnmVjhdBSDo98 Fig. S3A ------- COMMENT: d89a7babeb290b75 77 /voYlxikkjrZ4fz7XEUTVkV+ubc Fig. S3B ------- COMMENT: d89a7babeb290b75 78 cK86TZsYdAcGVMrZq6+Sao25Sok Fig. S5 ------- COMMENT: d8cd69d2eda564a7 17 iCOdnoKqePvSBdkoTwMOzq/LHRU (comment: CHECK same as cdc10-129 alone) ------- COMMENT: d8cd69d2eda564a7 18 W6ABl9xDpkeHDhSQSa00SyZKzZw (comment: CHECK same as cdc2-33 alone) ------- COMMENT: d8cd69d2eda564a7 20 N+GpfDy/gd1iZ7wMkatpJ6poXTU (comment: CHECK same as cdc13-117 alone) ------- COMMENT: d8cd69d2eda564a7 22 pKVxBj/MVK3XfFl6JfCX/N6cDxM (comment: CHECK same as rad4 cut5 allele alone) ------- COMMENT: d8cd69d2eda564a7 23 pKVxBj/MVK3XfFl6JfCX/N6cDxM (comment: CHECK same as rad4 cut5 allele alone) ------- COMMENT: d8cd69d2eda564a7 24 pKVxBj/MVK3XfFl6JfCX/N6cDxM (comment: CHECK same as rad4 cut5 allele alone) ------- COMMENT: d8cd69d2eda564a7 25 qq3zoXW7oQuBNIHqfthlt0i3ZOA (comment: CHECK same as cdc25-22 alone) ------- COMMENT: d8cd69d2eda564a7 27 pKVxBj/MVK3XfFl6JfCX/N6cDxM (comment: CHECK same as rad4 cut5 allele alone) ------- COMMENT: d8cd69d2eda564a7 28 pKVxBj/MVK3XfFl6JfCX/N6cDxM (comment: CHECK same as rad4 cut5 allele alone) ------- COMMENT: d8cd69d2eda564a7 29 pKVxBj/MVK3XfFl6JfCX/N6cDxM (comment: CHECK same as rad4 cut5 allele alone) ------- COMMENT: d8cd69d2eda564a7 30 pKVxBj/MVK3XfFl6JfCX/N6cDxM (comment: CHECK same as rad4 cut5 allele alone) ------- COMMENT: d8cd69d2eda564a7 31 pKVxBj/MVK3XfFl6JfCX/N6cDxM (comment: CHECK same as rad4 cut5 allele alone) ------- COMMENT: d8cd69d2eda564a7 32 pKVxBj/MVK3XfFl6JfCX/N6cDxM (comment: CHECK same as rad4 cut5 allele alone) ------- COMMENT: d8d0ae0fabd341f8 3 k4aJ/cz+2yW7jSVis+KQ4Ww7AUI (comment: CHECK Assays were done in the MDR-sup (multi-drug resistance-suppressed) genetic background together with nda3-TB101) ------- COMMENT: d8d0ae0fabd341f8 4 aqB0slJk8YMYW7Rg+iRxkO+GFu4 (comment: CHECK reduced frequency of microtubule catastrophe) ------- COMMENT: d8d0ae0fabd341f8 5 69UAjoDara6A7PCYZSzBvyw0vCo (comment: CHECK reduced frequency of microtubule rescue) ------- COMMENT: d8d0ae0fabd341f8 8 LDSQF7MfKWr1SN7SyycXvMqRGiY (comment: CHECK rescued by the nda3-TB101 allele) ------- COMMENT: d8d0ae0fabd341f8 9 a7D7KYJf33EAPX99ucPdQ5paTcM (comment: CHECK resistance to thiabendazole) ------- COMMENT: d8d0ae0fabd341f8 25 ZFF7KjepfkPUXwxxCVabVKbiOEA (comment: CHECK same as alp14delta alone) ------- COMMENT: d8d0ae0fabd341f8 27 ZFF7KjepfkPUXwxxCVabVKbiOEA (comment: CHECK same as alp14delta alone) ------- COMMENT: d8d0ae0fabd341f8 28 ZFF7KjepfkPUXwxxCVabVKbiOEA (comment: CHECK same as alp14delta alone) ------- COMMENT: d8d0ae0fabd341f8 29 ZFF7KjepfkPUXwxxCVabVKbiOEA (comment: CHECK same as alp14delta alone) ------- COMMENT: d8d0ae0fabd341f8 30 ZFF7KjepfkPUXwxxCVabVKbiOEA (comment: CHECK same as alp14delta alone) ------- COMMENT: d8d0ae0fabd341f8 40 /bSLX0mL8iVT4t6RX+ilb/zu4qg (comment: CHECK same as klp6delta alone) ------- COMMENT: d8d0ae0fabd341f8 41 /bSLX0mL8iVT4t6RX+ilb/zu4qg (comment: CHECK same as klp6delta alone) ------- COMMENT: d8f2845f7d04fc0b 2 8V4tblAfzihCFgwcgxItaf+j/hc ). Interestingly, growth of transformants over- expressing truncated Fxn1 with a disrupted mitochondrial localization sequence (Fxn1Δ2–11) is similar to pREP3X at all concentrations of thi- amine (Fig. 1A). These observations demonstrate that the growth inhi- bition resulting from Fxn1 overexpression is related to mitochondrial levels or improper processing of Fxn1. ------- COMMENT: d8f932ddae176e0b 1 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: d8f932ddae176e0b 2 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: d8f932ddae176e0b 3 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: d8f932ddae176e0b 4 p3x2dDTjQlS9jbO+IMHpHbED4nw (Fig. 1D) ------- COMMENT: d8f932ddae176e0b 6 p3x2dDTjQlS9jbO+IMHpHbED4nw (Fig. 1D) ------- COMMENT: d8f932ddae176e0b 7 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: d8f932ddae176e0b 9 +ztv57MbZRFnZbUybzwm4JTjc8k (Fig. 1E, F and G) ------- COMMENT: d8f932ddae176e0b 10 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: d8f932ddae176e0b 11 tdY+sTkcVXQXSK7tyg52iDlFg+Y (Fig. S1A) ------- COMMENT: d8f932ddae176e0b 12 d2NdxnatvYWNnh9afhcOiSOs0XA (Fig. S1D) ------- COMMENT: d8f932ddae176e0b 13 UecLDdbzEF2Fd34z5as/4/0I/eg (Fig. S1E) ------- COMMENT: d8f932ddae176e0b 14 3IyQZhdfRKA0GxRoOnA1cGYug+Y (Fig. 1F and G) ------- COMMENT: d8f932ddae176e0b 15 3IyQZhdfRKA0GxRoOnA1cGYug+Y (Fig. 1F and G) ------- COMMENT: d8f932ddae176e0b 16 3IyQZhdfRKA0GxRoOnA1cGYug+Y (Fig. 1F and G) ------- COMMENT: d8f932ddae176e0b 17 3IyQZhdfRKA0GxRoOnA1cGYug+Y (Fig. 1F and G) ------- COMMENT: d8f932ddae176e0b 18 3IyQZhdfRKA0GxRoOnA1cGYug+Y (Fig. 1F and G) ------- COMMENT: d8f932ddae176e0b 19 3IyQZhdfRKA0GxRoOnA1cGYug+Y (Fig. 1F and G) ------- COMMENT: d8f932ddae176e0b 20 3IyQZhdfRKA0GxRoOnA1cGYug+Y (Fig. 1F and G) ------- COMMENT: d8f932ddae176e0b 21 3IyQZhdfRKA0GxRoOnA1cGYug+Y (Fig. 1F and G) ------- COMMENT: d8f932ddae176e0b 22 3IyQZhdfRKA0GxRoOnA1cGYug+Y (Fig. 1F and G) ------- COMMENT: d8f932ddae176e0b 23 ehNYXIf0dqxYwNpXACqqZjiYgIg (Fig. 1F ang G) ------- COMMENT: d8f932ddae176e0b 24 UecLDdbzEF2Fd34z5as/4/0I/eg (Fig. S1E) ------- COMMENT: d8f932ddae176e0b 25 d2NdxnatvYWNnh9afhcOiSOs0XA (Fig. S1D) ------- COMMENT: d8f932ddae176e0b 26 W4xkP4H/sC1fm9WHOucBJfKfR7Y (Fig. 2) ------- COMMENT: d8f932ddae176e0b 27 W8PUYpe4l8g4tOXDe+H/laoo/Ws (Fig. 3A, B, C and D) ------- COMMENT: d8f932ddae176e0b 28 biv5vzYpNRqMrXY+5I3wXOO1GLA (Fig. 1G) ------- COMMENT: d8f932ddae176e0b 29 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: d8f932ddae176e0b 30 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: d8f932ddae176e0b 31 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: d8f932ddae176e0b 32 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: d8f932ddae176e0b 33 2c4p3mUUeTur77Gd44Gyo8/Q+tw (Fig. 3F and G) ------- COMMENT: d8f932ddae176e0b 34 RAQ9iCj6ucjGCQHQQosHYrKMC00 (Fig. 3I) ------- COMMENT: d8f932ddae176e0b 35 2c4p3mUUeTur77Gd44Gyo8/Q+tw (Fig. 3F and G) ------- COMMENT: d8f932ddae176e0b 36 2c4p3mUUeTur77Gd44Gyo8/Q+tw (Fig. 3F and G) ------- COMMENT: d8f932ddae176e0b 37 2c4p3mUUeTur77Gd44Gyo8/Q+tw (Fig. 3F and G) ------- COMMENT: d8f932ddae176e0b 38 2c4p3mUUeTur77Gd44Gyo8/Q+tw (Fig. 3F and G) ------- COMMENT: d8f932ddae176e0b 39 RAQ9iCj6ucjGCQHQQosHYrKMC00 (Fig. 3I) ------- COMMENT: d8f932ddae176e0b 40 RAQ9iCj6ucjGCQHQQosHYrKMC00 (Fig. 3I) ------- COMMENT: d8f932ddae176e0b 41 RAQ9iCj6ucjGCQHQQosHYrKMC00 (Fig. 3I) ------- COMMENT: d8f932ddae176e0b 42 RAQ9iCj6ucjGCQHQQosHYrKMC00 (Fig. 3I) ------- COMMENT: d8f932ddae176e0b 43 RAQ9iCj6ucjGCQHQQosHYrKMC00 (Fig. 3I) ------- COMMENT: d8f932ddae176e0b 45 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: d8f932ddae176e0b 46 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: d8f932ddae176e0b 47 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: d8f932ddae176e0b 48 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: d8f932ddae176e0b 50 kkBYIdh4MWpKs0ilnQlPhvbjZsU (Fig. 4H) ------- COMMENT: d8f932ddae176e0b 51 y7Yq/bA6j8EwABo1e7r6dVZsuN8 (Fig. 4I) ------- COMMENT: d8f932ddae176e0b 52 GkI93EYSqP6NybAtsJjxO2fy3Ic (Fig. 4D) ------- COMMENT: d8f932ddae176e0b 53 GkI93EYSqP6NybAtsJjxO2fy3Ic (Fig. 4D) ------- COMMENT: d8f932ddae176e0b 54 kkBYIdh4MWpKs0ilnQlPhvbjZsU (Fig. 4H) ------- COMMENT: d8f932ddae176e0b 55 y7Yq/bA6j8EwABo1e7r6dVZsuN8 (Fig. 4I) ------- COMMENT: d8f932ddae176e0b 59 GkI93EYSqP6NybAtsJjxO2fy3Ic (Fig. 4D) ------- COMMENT: d8f932ddae176e0b 60 5tEr19lZ889dGeKurZ925NdOZno (Fig. 4E and F) ------- COMMENT: d8f932ddae176e0b 62 5tEr19lZ889dGeKurZ925NdOZno (Fig. 4E and F) ------- COMMENT: d8f932ddae176e0b 63 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: d8f932ddae176e0b 64 xhsEwSjsS3WiGn8vmKaT8Vr1lUo (Fig. S2) ------- COMMENT: d8f932ddae176e0b 65 xhsEwSjsS3WiGn8vmKaT8Vr1lUo (Fig. S2) ------- COMMENT: d9011560b1887e36 17 jV2NOcbTpDgD7VFvtc2w38dlAiE (comment: CONDITION 30 degrees C) ------- COMMENT: d9011560b1887e36 18 jV2NOcbTpDgD7VFvtc2w38dlAiE (comment: CONDITION 30 degrees C) ------- COMMENT: d9011560b1887e36 22 jV2NOcbTpDgD7VFvtc2w38dlAiE (comment: CONDITION 30 degrees C) ------- COMMENT: d9011560b1887e36 23 jV2NOcbTpDgD7VFvtc2w38dlAiE (comment: CONDITION 30 degrees C) ------- COMMENT: d9011560b1887e36 38 ZQV9C+DHVDTsaGzmHj7VyTZcrKk (comment: 30 degrees; semi-permissive for slx8-29) ------- COMMENT: d9011560b1887e36 39 ZQV9C+DHVDTsaGzmHj7VyTZcrKk (comment: 30 degrees; semi-permissive for slx8-29) ------- COMMENT: d9011560b1887e36 40 ZQV9C+DHVDTsaGzmHj7VyTZcrKk (comment: 30 degrees; semi-permissive for slx8-29) ------- COMMENT: d9011560b1887e36 43 ZQV9C+DHVDTsaGzmHj7VyTZcrKk (comment: 30 degrees; semi-permissive for slx8-29) ------- COMMENT: d9011560b1887e36 50 ZQV9C+DHVDTsaGzmHj7VyTZcrKk (comment: 30 degrees; semi-permissive for slx8-29) ------- COMMENT: d9011560b1887e36 51 ZQV9C+DHVDTsaGzmHj7VyTZcrKk (comment: 30 degrees; semi-permissive for slx8-29) ------- COMMENT: d9011560b1887e36 52 ZQV9C+DHVDTsaGzmHj7VyTZcrKk (comment: 30 degrees; semi-permissive for slx8-29) ------- COMMENT: d9011560b1887e36 53 ZQV9C+DHVDTsaGzmHj7VyTZcrKk (comment: 30 degrees; semi-permissive for slx8-29) ------- COMMENT: d9011560b1887e36 59 ZQV9C+DHVDTsaGzmHj7VyTZcrKk (comment: 30 degrees; semi-permissive for slx8-29) ------- COMMENT: d9011560b1887e36 63 ZQV9C+DHVDTsaGzmHj7VyTZcrKk (comment: 30 degrees; semi-permissive for slx8-29) ------- COMMENT: d9011560b1887e36 64 ZQV9C+DHVDTsaGzmHj7VyTZcrKk (comment: 30 degrees; semi-permissive for slx8-29) ------- COMMENT: d9011560b1887e36 65 ZQV9C+DHVDTsaGzmHj7VyTZcrKk (comment: 30 degrees; semi-permissive for slx8-29) ------- COMMENT: d9011560b1887e36 66 ZQV9C+DHVDTsaGzmHj7VyTZcrKk (comment: 30 degrees; semi-permissive for slx8-29) ------- COMMENT: d9011560b1887e36 84 ZQV9C+DHVDTsaGzmHj7VyTZcrKk (comment: 30 degrees; semi-permissive for slx8-29) ------- COMMENT: d9011560b1887e36 88 ZQV9C+DHVDTsaGzmHj7VyTZcrKk (comment: 30 degrees; semi-permissive for slx8-29) ------- COMMENT: d9011560b1887e36 89 ZQV9C+DHVDTsaGzmHj7VyTZcrKk (comment: 30 degrees; semi-permissive for slx8-29) ------- COMMENT: d9011560b1887e36 90 ZQV9C+DHVDTsaGzmHj7VyTZcrKk (comment: 30 degrees; semi-permissive for slx8-29) ------- COMMENT: d905b7844f55c1d0 16 2Z4mEfZLaiBWRzYp20WO5dqBpd4 (comment: CHECK assayed at cdc18 and cdc22) ------- COMMENT: d905b7844f55c1d0 17 2Z4mEfZLaiBWRzYp20WO5dqBpd4 (comment: CHECK assayed at cdc18 and cdc22) ------- COMMENT: d905b7844f55c1d0 19 2Z4mEfZLaiBWRzYp20WO5dqBpd4 (comment: CHECK assayed at cdc18 and cdc22) ------- COMMENT: d905b7844f55c1d0 20 2Z4mEfZLaiBWRzYp20WO5dqBpd4 (comment: CHECK assayed at cdc18 and cdc22) ------- COMMENT: d905b7844f55c1d0 21 2Z4mEfZLaiBWRzYp20WO5dqBpd4 (comment: CHECK assayed at cdc18 and cdc22) ------- COMMENT: d905b7844f55c1d0 22 2Z4mEfZLaiBWRzYp20WO5dqBpd4 (comment: CHECK assayed at cdc18 and cdc22) ------- COMMENT: d905b7844f55c1d0 23 2Z4mEfZLaiBWRzYp20WO5dqBpd4 (comment: CHECK assayed at cdc18 and cdc22) ------- COMMENT: d905b7844f55c1d0 24 2Z4mEfZLaiBWRzYp20WO5dqBpd4 (comment: CHECK assayed at cdc18 and cdc22) ------- COMMENT: d905b7844f55c1d0 25 2Z4mEfZLaiBWRzYp20WO5dqBpd4 (comment: CHECK assayed at cdc18 and cdc22) ------- COMMENT: d905b7844f55c1d0 37 2Z4mEfZLaiBWRzYp20WO5dqBpd4 (comment: CHECK assayed at cdc18 and cdc22) ------- COMMENT: d905b7844f55c1d0 38 2Z4mEfZLaiBWRzYp20WO5dqBpd4 (comment: CHECK assayed at cdc18 and cdc22) ------- COMMENT: d905b7844f55c1d0 39 2Z4mEfZLaiBWRzYp20WO5dqBpd4 (comment: CHECK assayed at cdc18 and cdc22) ------- COMMENT: d91d901cb0a83596 2 0qtQ4wZAkGBN3jaNS6RQrc06zmk (comment: K63-ubiquitin chain from 3 to 8 ubiquitin molecules) ------- COMMENT: d91d901cb0a83596 3 bepkbBi0WBi1aCUom8qRIaV+IbQ (comment: K63-diubiquitin chain) ------- COMMENT: d91d901cb0a83596 11 2bcuiCxrjErHpGnQMX4J7D3/Mi4 (comment: Delete K63-ubiquitin chains from 3 to 8 ubiquitins) ------- COMMENT: d91d901cb0a83596 93 0qtQ4wZAkGBN3jaNS6RQrc06zmk (comment: K63-ubiquitin chain from 3 to 8 ubiquitin molecules) ------- COMMENT: d91d901cb0a83596 94 bepkbBi0WBi1aCUom8qRIaV+IbQ (comment: K63-diubiquitin chain) ------- COMMENT: d9659faec55f853e 58 iDRq5uBjot6W0MbUCGD0VhIyaGM Fig. 2f ------- COMMENT: d9659faec55f853e 59 iDRq5uBjot6W0MbUCGD0VhIyaGM Fig. 2f ------- COMMENT: d9659faec55f853e 60 R0ybwCfuPvIoUaX4v9l28IsZc4Y Fig. S2 ------- COMMENT: d96802a70862fa93 1 CQIfMFMZlug+Dj9XzQygC0Ch4rE Fig S10 (comment: tetrad analysis) ------- COMMENT: d96802a70862fa93 2 CQIfMFMZlug+Dj9XzQygC0Ch4rE Fig S10 (comment: tetrad analysis) ------- COMMENT: d96802a70862fa93 3 CQIfMFMZlug+Dj9XzQygC0Ch4rE Fig S10 (comment: tetrad analysis) ------- COMMENT: d96802a70862fa93 4 CQIfMFMZlug+Dj9XzQygC0Ch4rE Fig S10 (comment: tetrad analysis) ------- COMMENT: d96802a70862fa93 5 CQIfMFMZlug+Dj9XzQygC0Ch4rE Fig S10 (comment: tetrad analysis) ------- COMMENT: d96802a70862fa93 6 CQIfMFMZlug+Dj9XzQygC0Ch4rE Fig S10 (comment: tetrad analysis) ------- COMMENT: d96802a70862fa93 7 CQIfMFMZlug+Dj9XzQygC0Ch4rE Fig S10 (comment: tetrad analysis) ------- COMMENT: d96802a70862fa93 22 hv8TSZHrgqiIIXLuzwoW9zww/sM (comment: CHECK TBZ 15ug/ml) ------- COMMENT: d96802a70862fa93 23 hv8TSZHrgqiIIXLuzwoW9zww/sM (comment: CHECK TBZ 15ug/ml) ------- COMMENT: d96802a70862fa93 24 hv8TSZHrgqiIIXLuzwoW9zww/sM (comment: CHECK TBZ 15ug/ml) ------- COMMENT: d96802a70862fa93 25 hv8TSZHrgqiIIXLuzwoW9zww/sM (comment: CHECK TBZ 15ug/ml) ------- COMMENT: d96802a70862fa93 26 hv8TSZHrgqiIIXLuzwoW9zww/sM (comment: CHECK TBZ 15ug/ml) ------- COMMENT: d96802a70862fa93 29 OrtX9bdB4RQPiDtyPU/P5FK5/+Q Figure 5C (comment: forward strand RT-qPCR (dh repeat)) ------- COMMENT: d96802a70862fa93 30 OrtX9bdB4RQPiDtyPU/P5FK5/+Q Figure 5C (comment: forward strand RT-qPCR (dh repeat)) ------- COMMENT: d96802a70862fa93 31 uS8hg5MMNNnrRfXwVsIVoieJgMg Figure 5C (comment: forward strand RT-qPCR (dh repeat)) ------- COMMENT: d96802a70862fa93 32 uS8hg5MMNNnrRfXwVsIVoieJgMg Figure 5C (comment: forward strand RT-qPCR (dh repeat)) ------- COMMENT: d96802a70862fa93 33 uS8hg5MMNNnrRfXwVsIVoieJgMg Figure 5C (comment: forward strand RT-qPCR (dh repeat)) ------- COMMENT: d96802a70862fa93 34 uS8hg5MMNNnrRfXwVsIVoieJgMg Figure 5C (comment: forward strand RT-qPCR (dh repeat)) ------- COMMENT: d96802a70862fa93 42 QpUbB2AXylzHmWjM6tWk+F+3PuI Figure 5D ------- COMMENT: d96802a70862fa93 43 QpUbB2AXylzHmWjM6tWk+F+3PuI Figure 5D ------- COMMENT: d99000225d887e1f 1 y5Ioz1q179JKGWr1AWpMk9ee+rI Consistent with the results of the genetic screen, serial dilution analyses show that git1Δ, git3Δ, git5Δ, gpa2Δ, pka1Δ, and cyr1Δ all rescue silenc- ing defects of otr::ura4+ caused by Epe1 overexpression, as indicated by better growth on EMM medium containing 5-FOA (Fig 1D) ------- COMMENT: d99000225d887e1f 7 OHcRHZAOzd5SPVqcMbptYalx6Fs H3K9me2 levels at dh repeats are restored close to wild-type levels in git3Δ nmt41-epe1+ cells (Fig 1E). ------- COMMENT: d99000225d887e1f 21 v6XhvhkNpVLWf8ef4LUWFlrmx50 nterestingly, we found that Epe1 protein levels are significantly reduced in git3Δ nmt41-epe1+ and pka1Δ nmt41-epe1+ cells (Fig 2F) ------- COMMENT: d99000225d887e1f 22 ZnTF9QS8OLPI0u2omEP+yL4mkpU . Interestingly, although git3Δ nmt41-epe1+ cells form heterochromatin at pericentric repeats, ------- COMMENT: d99000225d887e1f 24 eCe44hPKDfiwWTRnZICuNHfqa/Y (comment: polysome profiling) ------- COMMENT: d99000225d887e1f 28 eCe44hPKDfiwWTRnZICuNHfqa/Y (comment: polysome profiling) ------- COMMENT: d99000225d887e1f 35 E3HXkGKmAvMiN8QFdhDGwf50Fnc figure 1b ------- COMMENT: d9b0a79b6b29212e 1 PjvCpjbKRpRoBAvwcylaOBD0748 (comment: assayed complex) ------- COMMENT: d9b0a79b6b29212e 2 PjvCpjbKRpRoBAvwcylaOBD0748 (comment: assayed complex) ------- COMMENT: d9b0a79b6b29212e 3 1biRlPTXTdTXWjYEad7C9T9sjP4 All six subunits of the Clr6S complex are seen in the map (Fig. 2 B and C and SI Appendix, Table S1). Unexpectedly, the structure also reveals two copies of Alp13 (hereafter referred to as Alp13a and Alp13b) (Fig. 2C) ------- COMMENT: d9b0a79b6b29212e 4 NvollpvcsX+AMsuM/Kv7nrOGc+M The structure shows that Pst2, Clr6, and Prw1, a WD40-containing subunit, form a subcomplex, within which Pst2 associates with the catalytic subunit Clr6, as well as interacting with Prw1 (Fig. 2C), ------- COMMENT: d9b0a79b6b29212e 5 NvollpvcsX+AMsuM/Kv7nrOGc+M The structure shows that Pst2, Clr6, and Prw1, a WD40-containing subunit, form a subcomplex, within which Pst2 associates with the catalytic subunit Clr6, as well as interacting with Prw1 (Fig. 2C), ------- COMMENT: d9b0a79b6b29212e 6 SMErLV4ob5Hm/KhjNssGEWW5NoA Unexpectedly, the structure also reveals two copies of Alp13 (hereafter referred to as Alp13a and Alp13b) (Fig. 2C), ------- COMMENT: d9b0a79b6b29212e 7 PG05tZq61PBSuzp+d8R8dmTcrpE Alp13b is connected to the complex by both Cph1 and Cph2 (Figs. 2C and 3A) ------- COMMENT: d9b0a79b6b29212e 8 PG05tZq61PBSuzp+d8R8dmTcrpE Alp13b is connected to the complex by both Cph1 and Cph2 (Figs. 2C and 3A) ------- COMMENT: d9b0a79b6b29212e 9 MFxG1jZBbvuIFurrnA8pY+q3NHk Pst2 evidently serves as a structural platform, bringing together almost all the other subunits of the complex. ------- COMMENT: d9b0a79b6b29212e 10 qAoO0cQCiEq1NiVkFQjEEW6FxdY Thus, Cph2 interacts with all the other subunits except Prw1, whose association with the complex occurs only through the Pst2–CTD (Fig. 4C). ------- COMMENT: d9b0a79b6b29212e 11 KvKT4RzKe8MMt+JXqTp10OUYno4 Deletion of the active center loop compromised the HDAC activity of Clr6S (Fig. 6G), consistent with a role of the loop in catalytic activity, although a secondary effect, due to perturbation of the protein structure could not be excluded., ------- COMMENT: d9b0a79b6b29212e 12 PjvCpjbKRpRoBAvwcylaOBD0748 (comment: assayed complex) ------- COMMENT: d9b0a79b6b29212e 13 PjvCpjbKRpRoBAvwcylaOBD0748 (comment: assayed complex) ------- COMMENT: d9f75c80dc374265 14 F0z10tx5I/UZ4dxpbIHVo3WH5Ds (comment: CHECK activated_by(CHEBI:17234)) ------- COMMENT: da18465232a360d6 1 m9102kTUiY2kz5cBPWUABBg/08E Among the 41 tested strains, three showed a markedly low YFP/RFP ratio when compared to the wild type strain: Δcwf12, Δsaf5 and Δsaf1. (Figure 2A) ------- COMMENT: da18465232a360d6 2 m9102kTUiY2kz5cBPWUABBg/08E Among the 41 tested strains, three showed a markedly low YFP/RFP ratio when compared to the wild type strain: Δcwf12, Δsaf5 and Δsaf1. (Figure 2A) ------- COMMENT: da18465232a360d6 5 m9102kTUiY2kz5cBPWUABBg/08E Among the 41 tested strains, three showed a markedly low YFP/RFP ratio when compared to the wild type strain: Δcwf12, Δsaf5 and Δsaf1. (Figure 2A) ------- COMMENT: da18465232a360d6 6 k2DZrIu2lNFqz9oZUXVr5wlU4P8 Of these candidates, only 4 were already known to be involved in the regulation of splicing (Cwf12, Saf5, Cwf19 and SPAC1705.02), whereas the remaining 33 were unrelated or not clearly assigned to the regula- tion of splicing ------- COMMENT: da18465232a360d6 7 k2DZrIu2lNFqz9oZUXVr5wlU4P8 Of these candidates, only 4 were already known to be involved in the regulation of splicing (Cwf12, Saf5, Cwf19 and SPAC1705.02), whereas the remaining 33 were unrelated or not clearly assigned to the regula- tion of splicing ------- COMMENT: da18465232a360d6 8 3tT5YkVHqo6qTI5FPMJtB39IxHo the effect observed in the Δmpn1 strain was comparable to that observed in Δsaf5 cells. ------- COMMENT: da18465232a360d6 10 keqw9gaCIgu7jBDDO5Mv9p9wptg As shown in S3A Fig, the amount of Mmi1 was significantly reduced in Δsaf5 cells to less than 50% (S3B Fig), when compared to a wild type strain. On the other hand, replacing the wild type mmi1 gene with a copy without introns (sfGFP-Mmi1 cDNA), nearly abolished the effect of the absence of Saf5 on the amount of Mmi1 (S3A and S3B Fig). ------- COMMENT: da18465232a360d6 11 4ig6vfbM4RNDRFzdZkTPNUnVlGI (comment: demonstrated by cDNA copy) ------- COMMENT: da18465232a360d6 12 PKBnfoBQsuHS/9lrypV4rhIvXWM Therefore, when Cdk9 was inactivated, transcription was slowed down [34,35]. We ana- lyzed the splicing of the single intron of cbf11, which is one of the most affected in the Δsaf5 strain, with 60% of intron retention. The addition of the bulky ATP analog barely affected intron retention in a wild type or a Δsaf5 strains (Fig 5A). However, upon adding 3-MB-PP1 to the culture of the cdk9as Δsaf5 strain, splicing of the cbf11 intron was noticeably improved, with intron retention reduced to 25%. Splicing also improved in the cdk9as saf5+ strain (depicted by the pale orange bars), probably as the result of decreasing transcription rate and giving more time for the splicing to occur; however, this improvement was not statistically sig- nificant. When we analyzed how inactivation of Cdk9 affected the splicing of multi-intronic genes, such as atg5 or urm1 (Fig 5B and 5C), we consistently observed improvements in all introns 30 minutes after adding 3-MB-PP1 ------- COMMENT: da18465232a360d6 13 Rr3kvH+WRJsDLVRwyJdwvmQ7p04 (comment: highly expressed genes (or conditionally expressed)) ------- COMMENT: da2ab48fecb84a55 1 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: da2ab48fecb84a55 2 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: da2ab48fecb84a55 3 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: da2ab48fecb84a55 4 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: da2ab48fecb84a55 5 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: da2ab48fecb84a55 6 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: da2ab48fecb84a55 16 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: da2ab48fecb84a55 17 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: da2ab48fecb84a55 18 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: da2ab48fecb84a55 19 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: da2ab48fecb84a55 20 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: da2ab48fecb84a55 21 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: da2ab48fecb84a55 22 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: da2ab48fecb84a55 23 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: da2ab48fecb84a55 24 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: da7b4175a523a7e1 1 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: da7b4175a523a7e1 2 PqfecLqBh98ICfzyvx3Z+CBwrQM Fig. 2 supplement 1A ------- COMMENT: da7b4175a523a7e1 5 1pIZvPz7gOb+AOntorbF0DHf7F8 In contrast, when we comple- mented the depleted extract with SpCAF-1 mutant complexes SpCAF-1-ED*, SpCAF-1-KER*, SpCAF- 1-ΔWHD we did not detect the supercoiled form I. This indicates that these mutants cannot promote nucleosome assembly (Figure 5). ------- COMMENT: da7b4175a523a7e1 6 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: da7b4175a523a7e1 7 UzQ0N0tHM6pSYyaIVOEK9eQFbxw Fig. 7B and C ------- COMMENT: da7b4175a523a7e1 8 i+bAoMpiE+e0BjHLMB4RiB+n3dQ Fig. 6C and D ------- COMMENT: da7b4175a523a7e1 9 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: da7b4175a523a7e1 10 yqCdE6uzM/fiVZPXpeC5yzFPH+k When we used the SpCAF-1-PIP* mutant, we did not detect super- coiling on labeled DNA at 45 min, yet at 2 hr supercoiling ultimately reached levels achieved using the WT SpCAF-1 (Figure 5, bottom, synthesized DNA). ------- COMMENT: da7b4175a523a7e1 11 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: da7b4175a523a7e1 12 i+bAoMpiE+e0BjHLMB4RiB+n3dQ Fig. 6C and D ------- COMMENT: da7b4175a523a7e1 13 UzQ0N0tHM6pSYyaIVOEK9eQFbxw Fig. 7B and C ------- COMMENT: da7b4175a523a7e1 14 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: da7b4175a523a7e1 15 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: da7b4175a523a7e1 16 MCtrRQtVd2nG3qNfLtakucAIPRQ The KER* mutation was then introduced in the full complex SpCAF-1-KER* (Figure 1—figure supplement 1B, Figure 2— figure supplement 2B) and we confirmed by MST and EMSA its lower affinity for dsDNA (Figure 3B, Figure 3—figure supplement 2G, Table 1). ------- COMMENT: da7b4175a523a7e1 17 1pIZvPz7gOb+AOntorbF0DHf7F8 In contrast, when we comple- mented the depleted extract with SpCAF-1 mutant complexes SpCAF-1-ED*, SpCAF-1-KER*, SpCAF- 1-ΔWHD we did not detect the supercoiled form I. This indicates that these mutants cannot promote nucleosome assembly (Figure 5). ------- COMMENT: da7b4175a523a7e1 18 i+bAoMpiE+e0BjHLMB4RiB+n3dQ Fig. 6C and D ------- COMMENT: da7b4175a523a7e1 19 UzQ0N0tHM6pSYyaIVOEK9eQFbxw Fig. 7B and C ------- COMMENT: da7b4175a523a7e1 20 1pIZvPz7gOb+AOntorbF0DHf7F8 In contrast, when we comple- mented the depleted extract with SpCAF-1 mutant complexes SpCAF-1-ED*, SpCAF-1-KER*, SpCAF- 1-ΔWHD we did not detect the supercoiled form I. This indicates that these mutants cannot promote nucleosome assembly (Figure 5). ------- COMMENT: da7b4175a523a7e1 22 wr5TXzzUQ5a8pjtOfW1cRWvVFaY Fig. 7D ------- COMMENT: da7b4175a523a7e1 23 wr5TXzzUQ5a8pjtOfW1cRWvVFaY Fig. 7D ------- COMMENT: da7b4175a523a7e1 24 wr5TXzzUQ5a8pjtOfW1cRWvVFaY Fig. 7D ------- COMMENT: da7b4175a523a7e1 25 wr5TXzzUQ5a8pjtOfW1cRWvVFaY Fig. 7D ------- COMMENT: da7b4175a523a7e1 26 3BDTr8Z2/2bHwJiyw6XwkFcqJW4 Mixing the subunits by pairs, we observed by size exclusion chromatography (SEC), stable complexes for Pcf1-Pcf2 and Pcf1-Pcf3 (Figure 1—figure supplement 2A). ------- COMMENT: da7b4175a523a7e1 27 3BDTr8Z2/2bHwJiyw6XwkFcqJW4 Mixing the subunits by pairs, we observed by size exclusion chromatography (SEC), stable complexes for Pcf1-Pcf2 and Pcf1-Pcf3 (Figure 1—figure supplement 2A). ------- COMMENT: da7b4175a523a7e1 28 PqfecLqBh98ICfzyvx3Z+CBwrQM Fig. 2 supplement 1A ------- COMMENT: da7b4175a523a7e1 29 aTiW1oCuokH5FZArHjC1Iz10zKU In addition, the NMR signals of all IDR for this mutant with or without histones were close to that of the WT (Figure 3—figure supplement 3A–B) indicating that the KER* mutation did not impair histone binding. ------- COMMENT: da7b4175a523a7e1 30 aTiW1oCuokH5FZArHjC1Iz10zKU In addition, the NMR signals of all IDR for this mutant with or without histones were close to that of the WT (Figure 3—figure supplement 3A–B) indicating that the KER* mutation did not impair histone binding. ------- COMMENT: da7b4175a523a7e1 31 ByqiP/Nvcho0GS2zVoK/1p+VU0s We observed the similar DNA binding property and IDR properties for SpCAF-1-ΔWHD and the WT complex (Table 1, Figure 3B, Figure 3—figure supple- ment 2G, Figure 3—figure supplement 3A–B). ------- COMMENT: da7b4175a523a7e1 32 6QOQfVm0410SJkiEp5lg21rL1Eo Fig. 6 ------- COMMENT: da7b4175a523a7e1 33 i+bAoMpiE+e0BjHLMB4RiB+n3dQ Fig. 6C and D ------- COMMENT: da7b4175a523a7e1 34 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: da7b4175a523a7e1 35 UzQ0N0tHM6pSYyaIVOEK9eQFbxw Fig. 7B and C ------- COMMENT: da7b4175a523a7e1 36 UzQ0N0tHM6pSYyaIVOEK9eQFbxw Fig. 7B and C ------- COMMENT: dae563ad8708026c 13 MrRlDZ1J56Xtyp8m/NugQYcmfTA (comment: even though cdc13 is present) ------- COMMENT: dae563ad8708026c 15 04AWpUIlr8PNKej75b8dFVaH0t4 (comment: premature SIN) ------- COMMENT: db0ab2817f38d5f6 92 cbJkbRS1YENLI6coTeQeqBhWKsk (comment: CHECK candidate for involved_in_or_involved_in_regulation_of qualifier) ------- COMMENT: db3533d819cff33d 18 Od4AxikB21D7OX8hc9djDIGNw9c Sal3 is required for the nuclear import of Clp1 as shown by microscopy. ------- COMMENT: db3533d819cff33d 19 ElRGPU7dE7jZh2g/H0SPr7VWBKA Localization of Clp1 to nucleoplasm requires the presence of the nuclear localization sequence (NLS), which was identified to locate right at the end of the C-terminal. ------- COMMENT: db3533d819cff33d 21 OboWL8cSJYkm7Ymw3R233d+DfJM (comment: CHECK S244, S278, S501, S755, T831, and S852 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 22 5ftSNE79GCiN0z1Fs5i1WcbheCw (comment: CHECK Phosphorylation site S265 was identified by mass spectrometry.) ------- COMMENT: db3533d819cff33d 23 29CL76Po+oY0iDBqqLOBE3xNihw (comment: CHECK S332, S700, and S732 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 24 Kw0yT563oS1BoD/bPbcaNkFvkXs (comment: CHECK T123 and S334 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 25 nJrS9WLZeM+AwthKM5gxJygXRZk (comment: CHECK S118, S143, and T379 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 26 zTMuFrRkc8P6fW1YFFPVa1oQeu8 (comment: CHECK S48, S71, S103, S113, S140, S171, S195, S206, S221, S236, T240, T255, S257, S289, S344, S379, S399, and T411 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 27 8WonbGUeFxFOJTaF3dEY7LLfay8 (comment: CHECK S267 was identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 28 m7CPKknGNY+BPLhrluMXoYpHw2w (comment: CHECK S321 was identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 29 jIOCDDuWzYAo/2oZaOJqLdgmyG4 (comment: CHECK T297 and S364 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 30 rFth8EEkHV/ohZxWxzfnZ6S/mm8 (comment: CHECK S303 was identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 31 qYg3H4B15wDjV7ekUJxJmBDKbIQ (comment: CHECK S57 and S206 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 32 agthFKOUxDhBwkXvf7d/qPbS78k (comment: CHECK S183 and S372 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 33 iY6ScKxDJBZ+rkeERL5Mx144QbU (comment: CHECK S674 was identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 34 vUG/KnCcRQE6jAbLUbxojBvBHU8 (comment: CHECK S502 was identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 35 p514DsK0SCridsnWI0lKusZ6Tus (comment: CHECK S196 and S252 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 36 GCnKwIfx1TWDKbWIGJyxFKfuK5o (comment: CHECK T61, T71, S75, S156, S171, S361, S497, and S947 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 37 CTKzTdBN8FauRdn1v/CRMkIybCg (comment: CHECK S345 was identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 38 o0O85Fl5JXlDYVmkU5nSjAOQsVU (comment: CHECK S430, T451, S479, S491, T509, and T577 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 39 1TyOOGNluTxFjQqpL7zFfLtXcBo (comment: CHECK S147, S242, S270, S316, and S354 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 40 xg5eCzYrRSLIzM40OhLMINisLm4 (comment: CHECK S411 was identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 41 ADWN5zRl6EmUf7amBMO9OmqwBHA (comment: CHECK T554 was identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 42 DQ3i5LmTdR0orGvcGmGkVA6kcng (comment: CHECK S372 was identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 43 42LClbvMK4TVPKcquy87EWbGTlc (comment: CHECK S436 was identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 44 z2k9vytWsFsmxpebZIi7DNh8bvE (comment: CHECK S220 was identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 45 dOZhO2VPc6tM1CVzuYmq8DUWrn0 (comment: CHECK T106 was identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 46 6gA+SO7jj8fYrMNoDFpYKX2np5k (comment: CHECK S148 was identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 47 soNEeylt2pEk41YfWcwP1VdyPfM (comment: CHECK S65 was identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 48 QJzFY++4N8N2VJnU7ldin10oNDA (comment: CHECK S216 and S298 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 49 +6eYZ7RO7/aQYcaJh+r5QGwsSyQ (comment: CHECK S301 and S499 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 50 8TirtIS3DyhsRSDNu+tEVnL7LtY (comment: CHECK S558 was identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 51 fVXRdAJTthxpYLflT1WdsFZ73G8 (comment: CHECK S74 and S95 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 52 I0bygi6XEuqpmMKb6Lb4GRx+Wlk (comment: CHECK S370 was identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 92 TffAzqZbXpc8+DFHKPPIPGpB9qQ (comment: serine residues, presumably some or all of those mutated) ------- COMMENT: db3533d819cff33d 93 TffAzqZbXpc8+DFHKPPIPGpB9qQ (comment: serine residues, presumably some or all of those mutated) ------- COMMENT: db3533d819cff33d 94 TffAzqZbXpc8+DFHKPPIPGpB9qQ (comment: serine residues, presumably some or all of those mutated) ------- COMMENT: db3533d819cff33d 103 OboWL8cSJYkm7Ymw3R233d+DfJM (comment: CHECK S244, S278, S501, S755, T831, and S852 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 104 GCnKwIfx1TWDKbWIGJyxFKfuK5o (comment: CHECK T61, T71, S75, S156, S171, S361, S497, and S947 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 105 jIOCDDuWzYAo/2oZaOJqLdgmyG4 (comment: CHECK T297 and S364 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 106 Kw0yT563oS1BoD/bPbcaNkFvkXs (comment: CHECK T123 and S334 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 107 nJrS9WLZeM+AwthKM5gxJygXRZk (comment: CHECK S118, S143, and T379 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 108 Kw0yT563oS1BoD/bPbcaNkFvkXs (comment: CHECK T123 and S334 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 109 nJrS9WLZeM+AwthKM5gxJygXRZk (comment: CHECK S118, S143, and T379 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 110 o0O85Fl5JXlDYVmkU5nSjAOQsVU (comment: CHECK S430, T451, S479, S491, T509, and T577 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db3533d819cff33d 111 zTMuFrRkc8P6fW1YFFPVa1oQeu8 (comment: CHECK S48, S71, S103, S113, S140, S171, S195, S206, S221, S236, T240, T255, S257, S289, S344, S379, S399, and T411 were identified as phosphorylation sites by mass spectrometry.) ------- COMMENT: db89111ac3a96189 1 nt/Y7zSDPWkShYnsbZexxwtmr8I (Fig. 1C ------- COMMENT: db89111ac3a96189 2 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: db89111ac3a96189 3 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: db89111ac3a96189 4 aM4wyEH2hyIQbEPrbIhzCn4P1aA (Fig. 1F ------- COMMENT: db89111ac3a96189 5 aM4wyEH2hyIQbEPrbIhzCn4P1aA (Fig. 1F ------- COMMENT: db89111ac3a96189 6 gbsEZDL2WsTbMBJ8nTkh0vGNYlA fig 2A ------- COMMENT: db89111ac3a96189 7 gbsEZDL2WsTbMBJ8nTkh0vGNYlA fig 2A ------- COMMENT: db89111ac3a96189 8 gbsEZDL2WsTbMBJ8nTkh0vGNYlA fig 2A ------- COMMENT: db89111ac3a96189 9 BrfIbYRWSQXRJuEBq4H5V6IodbY fig 2B ------- COMMENT: db89111ac3a96189 10 o9LfcExe7QLxPGKhYiLbwsmshlo fig 2C ------- COMMENT: db89111ac3a96189 11 o9LfcExe7QLxPGKhYiLbwsmshlo fig 2C ------- COMMENT: db89111ac3a96189 12 o9LfcExe7QLxPGKhYiLbwsmshlo fig 2C ------- COMMENT: db89111ac3a96189 13 o9LfcExe7QLxPGKhYiLbwsmshlo fig 2C ------- COMMENT: db89111ac3a96189 17 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: db89111ac3a96189 18 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: db89111ac3a96189 19 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: db89111ac3a96189 20 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: db89111ac3a96189 21 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: db89111ac3a96189 23 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: db89111ac3a96189 24 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: db89111ac3a96189 25 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: db89111ac3a96189 26 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: db8f8f5d2b131ec2 4 SDubuTc5kxyuJd4LOCHxf7Atdf8 (comment: CHECK microscopy shows "protein localization to vacuole with protein mislocalized to cytosol" but with additional vacuolar processing phenotypes I think we can make the BP phenotypes /AL) ------- COMMENT: db8f8f5d2b131ec2 40 3TOghLoiPdIxsKqWL3elkgAXvIs Fig. s3 ------- COMMENT: db8f8f5d2b131ec2 41 3TOghLoiPdIxsKqWL3elkgAXvIs Fig. s3 ------- COMMENT: db8f8f5d2b131ec2 51 UULTDBRxxNgwjgecd/1Q5zFHzXQ (comment: CHECK macroautophagy? - selective autophagy is a child of macroautophagy) ------- COMMENT: db8f8f5d2b131ec2 69 FhB9tdM4xQS/vJ5kET8b6DqRnJA (comment: 4h) ------- COMMENT: db8f8f5d2b131ec2 70 wJRWgA5W+pMhe9Jbe8pPsXAyd8M (comment: Indirect evidence, could be upstream) ------- COMMENT: db8f8f5d2b131ec2 71 wJRWgA5W+pMhe9Jbe8pPsXAyd8M (comment: Indirect evidence, could be upstream) ------- COMMENT: db8f8f5d2b131ec2 72 wJRWgA5W+pMhe9Jbe8pPsXAyd8M (comment: Indirect evidence, could be upstream) ------- COMMENT: db997f5a4f033da9 1 V8aY9cMSuflWPDnZXK1Ej8D/EEk fig 1b ------- COMMENT: db997f5a4f033da9 2 V8aY9cMSuflWPDnZXK1Ej8D/EEk fig 1b ------- COMMENT: db997f5a4f033da9 3 GcXXnUcEoxiBmWEd2k4rZvec/UI fig S1c ------- COMMENT: db997f5a4f033da9 4 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: db997f5a4f033da9 5 qW0fooJwQWtfFzpmyf80rEliP/A fig 5c ------- COMMENT: db997f5a4f033da9 6 UPB06S0UZaEo846Jtj/IyEP1A4U Fig 5c ------- COMMENT: db997f5a4f033da9 7 Pb3vD6JfIF6SyI40Izb1tpIzECw Fig 4b, c ------- COMMENT: db997f5a4f033da9 10 +XYiwbzDaT55zEhuVA6yZAtIxV4 Fig 1c, Fig 4e ------- COMMENT: db997f5a4f033da9 11 BmW+9WvSxWYKt1zKGxZq+C+AZzQ pericentric Fig 1c, In conclusion, while other tethering mechanisms in S. pombe could be functionally coupled to heterochroma- tin, the LEM-mediated centromere recruitment and the MSC-dependent silencing are independent mechanisms, although they are mediated by the same protein. ------- COMMENT: db997f5a4f033da9 12 8aC90IquHyePWwpH3EH/ddhBbhw Fig 1c (comment: CHECK SHOULD BRE ORGANIZATION) ------- COMMENT: db997f5a4f033da9 15 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: db997f5a4f033da9 16 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: db997f5a4f033da9 17 gtZhTGNqwve5IcXw8+0fjeranwI fig 6d ------- COMMENT: db997f5a4f033da9 18 X99JGex3oL7roEou4KGWIhPnrZ0 fig 6e ------- COMMENT: db997f5a4f033da9 19 INyyalFSog2xVDY7sXhapZv3Fmc fig 6c ------- COMMENT: db997f5a4f033da9 20 INyyalFSog2xVDY7sXhapZv3Fmc fig 6c ------- COMMENT: db997f5a4f033da9 21 X99JGex3oL7roEou4KGWIhPnrZ0 fig 6e ------- COMMENT: db997f5a4f033da9 22 gtZhTGNqwve5IcXw8+0fjeranwI fig 6d ------- COMMENT: db997f5a4f033da9 23 gtZhTGNqwve5IcXw8+0fjeranwI fig 6d ------- COMMENT: db997f5a4f033da9 24 gtZhTGNqwve5IcXw8+0fjeranwI fig 6d ------- COMMENT: db997f5a4f033da9 25 X99JGex3oL7roEou4KGWIhPnrZ0 fig 6e ------- COMMENT: db997f5a4f033da9 26 X99JGex3oL7roEou4KGWIhPnrZ0 fig 6e ------- COMMENT: db997f5a4f033da9 27 23A7gKYH++mwxpDaLPxFOcAYx6Y fig 3c ------- COMMENT: db997f5a4f033da9 30 YGTHdFPWAmmR96d6Ldv0NPUQzEU Fig 3A ------- COMMENT: db997f5a4f033da9 31 YGTHdFPWAmmR96d6Ldv0NPUQzEU Fig 3A ------- COMMENT: db997f5a4f033da9 32 XitM7mCzJch6HZbgPvLJZYCEdZA Fig S4a ------- COMMENT: db997f5a4f033da9 33 CyCFojxFmlSsGAFqo69Xv4vC2Ls Fig S4c ------- COMMENT: db997f5a4f033da9 34 yThDJQFZX1hJTJqF08iANDrYmfg Fig 7d ------- COMMENT: db997f5a4f033da9 35 yThDJQFZX1hJTJqF08iANDrYmfg Fig 7d ------- COMMENT: db997f5a4f033da9 36 yThDJQFZX1hJTJqF08iANDrYmfg Fig 7d ------- COMMENT: db997f5a4f033da9 37 YpbDjFxfre0r6EdbM67RlSJ8LA0 Fig 7c ------- COMMENT: db997f5a4f033da9 38 YpbDjFxfre0r6EdbM67RlSJ8LA0 Fig 7c ------- COMMENT: db997f5a4f033da9 39 Rna8PvX/GFZDp7qJnVv+xgSz/UU Fig S5c ------- COMMENT: db997f5a4f033da9 40 mmvWVVqdqXM3rUn6NSbwwq0p0iw Fig 3b ------- COMMENT: db997f5a4f033da9 41 YA9xQmX2rcEiDsER90pVSNyP5tI fig 6a (comment: CHECK in combination with csi1∆; phenocopies lem2∆ csi1∆) ------- COMMENT: db997f5a4f033da9 42 DxB/NSwKYBalDnfWef80atDuMf4 Fig 7b ------- COMMENT: db997f5a4f033da9 43 DxB/NSwKYBalDnfWef80atDuMf4 Fig 7b ------- COMMENT: db997f5a4f033da9 44 qW0fooJwQWtfFzpmyf80rEliP/A fig 5c ------- COMMENT: db997f5a4f033da9 45 0qELcA1SirTIcCVuP9VbutzcvKY Fig 5b ------- COMMENT: db997f5a4f033da9 46 0qELcA1SirTIcCVuP9VbutzcvKY Fig 5b ------- COMMENT: db997f5a4f033da9 47 0qELcA1SirTIcCVuP9VbutzcvKY Fig 5b ------- COMMENT: db997f5a4f033da9 48 0qELcA1SirTIcCVuP9VbutzcvKY Fig 5b ------- COMMENT: db997f5a4f033da9 49 CxBctyvEwuASpl2s7zUJ4vghSkg Fig S2b (comment: CHECK partial rescue) ------- COMMENT: db997f5a4f033da9 50 bveXP1+UKYlu+Xw9trv2qV3Cq7g Fig 1e (comment: CHECK partial rescue) ------- COMMENT: db997f5a4f033da9 51 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: db997f5a4f033da9 52 EK3cJaIpApHaZfDj/il8fbVV8BY fig S2 ------- COMMENT: db997f5a4f033da9 53 EK3cJaIpApHaZfDj/il8fbVV8BY fig S2 ------- COMMENT: db997f5a4f033da9 54 EaJ1cIJCgxH1h7dmT6mEA3YTf4A Fig. 1d ------- COMMENT: db997f5a4f033da9 55 EaJ1cIJCgxH1h7dmT6mEA3YTf4A Fig. 1d ------- COMMENT: db997f5a4f033da9 56 EaJ1cIJCgxH1h7dmT6mEA3YTf4A Fig. 1d ------- COMMENT: db997f5a4f033da9 57 EaJ1cIJCgxH1h7dmT6mEA3YTf4A Fig. 1d ------- COMMENT: db997f5a4f033da9 58 acGVpmK5ESo19l4S0TGlsnyofys Fig. S4 ------- COMMENT: db997f5a4f033da9 59 EK3cJaIpApHaZfDj/il8fbVV8BY fig S2 ------- COMMENT: db997f5a4f033da9 60 EK3cJaIpApHaZfDj/il8fbVV8BY fig S2 ------- COMMENT: db997f5a4f033da9 61 EK3cJaIpApHaZfDj/il8fbVV8BY fig S2 ------- COMMENT: db997f5a4f033da9 62 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: db997f5a4f033da9 63 QgVxTN/hTtnj0DDd94h3gblk9RA Fig. s2 ------- COMMENT: db997f5a4f033da9 64 QgVxTN/hTtnj0DDd94h3gblk9RA Fig. s2 ------- COMMENT: db997f5a4f033da9 65 QgVxTN/hTtnj0DDd94h3gblk9RA Fig. s2 ------- COMMENT: db997f5a4f033da9 66 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: db997f5a4f033da9 67 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: db997f5a4f033da9 68 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: db997f5a4f033da9 69 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: db997f5a4f033da9 70 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: db997f5a4f033da9 71 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: db997f5a4f033da9 72 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: db997f5a4f033da9 73 3hLt4TyV+DGXZ24uOFRJOFJ9TW4 Fig. s5a ------- COMMENT: db997f5a4f033da9 74 r/R8vI6TkLOmQr47F7QOhGGegy4 Fig. 3b ------- COMMENT: db997f5a4f033da9 75 r/R8vI6TkLOmQr47F7QOhGGegy4 Fig. 3b ------- COMMENT: db997f5a4f033da9 76 r/R8vI6TkLOmQr47F7QOhGGegy4 Fig. 3b ------- COMMENT: db997f5a4f033da9 77 r/R8vI6TkLOmQr47F7QOhGGegy4 Fig. 3b ------- COMMENT: db997f5a4f033da9 78 23A7gKYH++mwxpDaLPxFOcAYx6Y fig 3c ------- COMMENT: db997f5a4f033da9 79 23A7gKYH++mwxpDaLPxFOcAYx6Y fig 3c ------- COMMENT: db997f5a4f033da9 80 W4egHp8nZtd5iNYpwU05IAEyjwY fig 3d ------- COMMENT: db997f5a4f033da9 81 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: db997f5a4f033da9 82 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: db997f5a4f033da9 84 jgPxh0S8rof4lSz1TG30wnuWFWk Fig. 4e ------- COMMENT: db997f5a4f033da9 85 jgPxh0S8rof4lSz1TG30wnuWFWk fig. 4e ------- COMMENT: db997f5a4f033da9 86 jgPxh0S8rof4lSz1TG30wnuWFWk Fig. 4e ------- COMMENT: db997f5a4f033da9 87 rDR41po8gfpi5g9cNpYWWk5easQ Fig. 5 ------- COMMENT: db997f5a4f033da9 88 rDR41po8gfpi5g9cNpYWWk5easQ Fig. 5 ------- COMMENT: db997f5a4f033da9 89 DxB/NSwKYBalDnfWef80atDuMf4 Fig 7b ------- COMMENT: db997f5a4f033da9 90 DxB/NSwKYBalDnfWef80atDuMf4 Fig 7b ------- COMMENT: db997f5a4f033da9 91 DxB/NSwKYBalDnfWef80atDuMf4 Fig 7b ------- COMMENT: db997f5a4f033da9 92 DxB/NSwKYBalDnfWef80atDuMf4 Fig 7b ------- COMMENT: db997f5a4f033da9 93 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: db997f5a4f033da9 94 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: db997f5a4f033da9 95 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: db997f5a4f033da9 96 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: db997f5a4f033da9 97 kCujzaGIOAFZS24bRSeQzFOUj9o Fig. 7 ------- COMMENT: db997f5a4f033da9 98 kCujzaGIOAFZS24bRSeQzFOUj9o Fig. 7 ------- COMMENT: db997f5a4f033da9 99 kCujzaGIOAFZS24bRSeQzFOUj9o Fig. 7 ------- COMMENT: db997f5a4f033da9 100 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: db997f5a4f033da9 101 kCujzaGIOAFZS24bRSeQzFOUj9o Fig. 7 ------- COMMENT: db997f5a4f033da9 102 2nEa2hNfNgXVLmIGR955sIGjyFg Fig. s10 ------- COMMENT: db997f5a4f033da9 103 2nEa2hNfNgXVLmIGR955sIGjyFg Fig. s10 ------- COMMENT: db997f5a4f033da9 104 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: db997f5a4f033da9 105 2nEa2hNfNgXVLmIGR955sIGjyFg Fig. s10 ------- COMMENT: db997f5a4f033da9 106 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: db997f5a4f033da9 108 MZcfVo/YKraS9faiLeGrUA9XedA Fig 5c. (comment: CHECK parent GO:0003682?) ------- COMMENT: db997f5a4f033da9 109 MZcfVo/YKraS9faiLeGrUA9XedA Fig 5c. (comment: CHECK parent GO:0003682?) ------- COMMENT: db9b5a7b5f36c25c 1 joUktSPUGXl1ezDJB/dL25jTXFA figure1 ------- COMMENT: db9b5a7b5f36c25c 2 joUktSPUGXl1ezDJB/dL25jTXFA figure1 ------- COMMENT: db9b5a7b5f36c25c 3 joUktSPUGXl1ezDJB/dL25jTXFA figure1 ------- COMMENT: db9b5a7b5f36c25c 4 joUktSPUGXl1ezDJB/dL25jTXFA figure1 ------- COMMENT: db9b5a7b5f36c25c 5 joUktSPUGXl1ezDJB/dL25jTXFA figure1 ------- COMMENT: db9b5a7b5f36c25c 6 eKlQdeiq1wjkh3T9WNqFatVfqR0 figure2a ------- COMMENT: db9b5a7b5f36c25c 7 eKlQdeiq1wjkh3T9WNqFatVfqR0 figure2a ------- COMMENT: db9b5a7b5f36c25c 8 vR4QHsxCJEwQST2EeYj9K2Gc4Ts figure2b ------- COMMENT: db9b5a7b5f36c25c 9 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: db9b5a7b5f36c25c 10 prdWJmH3UevRBpsJRs+jRe8QaBY Figure 2C, 3b ------- COMMENT: db9b5a7b5f36c25c 11 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: db9b5a7b5f36c25c 12 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: db9b5a7b5f36c25c 13 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: db9b5a7b5f36c25c 14 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: db9b5a7b5f36c25c 15 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: db9b5a7b5f36c25c 16 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: db9b5a7b5f36c25c 17 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: db9b5a7b5f36c25c 18 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: db9b5a7b5f36c25c 19 R0ybwCfuPvIoUaX4v9l28IsZc4Y Fig. S2 ------- COMMENT: db9b5a7b5f36c25c 20 RJTc6Zfvc0VpRhyxVmWejVhxYkI Figure 2D. Although deletion of moa1+ thus causes a centro- mere-specific defect, recombination appears to promote re- ductional segregation in moa1D cells because the defect in monopolar attachment is lessened in diploid recombination- proficient meiosis compared to haploid meiosis or diploid ------- COMMENT: db9b5a7b5f36c25c 21 s0+F6pT9p79d2MCNfSsV/O9QJuI Whereas monopolar attachment is obviously impaired in moa1D rec12D meiosis I, the protection of centromere co- hesion also appears defective since almost all sister chroma- tids eventually separate. ------- COMMENT: db9b5a7b5f36c25c 22 s0+F6pT9p79d2MCNfSsV/O9QJuI Whereas monopolar attachment is obviously impaired in moa1D rec12D meiosis I, the protection of centromere co- hesion also appears defective since almost all sister chroma- tids eventually separate. ------- COMMENT: db9b5a7b5f36c25c 24 KhL0yGokn60YuRKo6YA1+kHaoec (comment: induced) ------- COMMENT: db9b5a7b5f36c25c 28 lVvMgkFNoEp4aURnzoIpcy7J//0 fig 5A ------- COMMENT: db9b5a7b5f36c25c 29 3wU29nbcWjpZG/O/aG5us+Fhjmg Figure 5C However, the ChIP assay demonstrated intact lo- calization of Moa1 in rec8D meiotic cells (Figure 5C). Instead, we discovered that Moa1 localization was abolished in cells lacking CENP-C (Cnp3) (Figure 5C), ------- COMMENT: db9b5a7b5f36c25c 30 3wU29nbcWjpZG/O/aG5us+Fhjmg Figure 5C However, the ChIP assay demonstrated intact lo- calization of Moa1 in rec8D meiotic cells (Figure 5C). Instead, we discovered that Moa1 localization was abolished in cells lacking CENP-C (Cnp3) (Figure 5C), ------- COMMENT: db9b5a7b5f36c25c 32 BqEAgV/uIJt5ruPwFUmnFLrEjVY Surprisingly, moa1D cells displayed slightly stronger signals of Rec8-GFP at the cluster of centromeres (Figure 6A, GFP dots in the nucleus). Subsequent ChIP assays revealed that the association of Rec8 to chromatin increased nearly 2-fold in moa1D cells, particularly at the centromeric central core region (Figure 6B). ------- COMMENT: db9b5a7b5f36c25c 33 kMoDAhh5qRK6L2X983qVqNWYL5Q This hypothesis makes the key prediction that the in- crease of Rec8 at the centromeric central core would de- pend on DNA replication. To test this possibility, we blocked DNA replication by adding HU to the synchronized meiotic culture and examined by ChIP the localization pattern of Rec8 (Figure 6C). Levels of central core-associated Rec8 were the same before (+HU) or after DNA replication (-HU) in wild-type cells. Remarkably, HU treatment abolished the increase of central core Rec8 in moa1D cells, and the pattern became similar to that in moa1+ cells (Figure 6C, +HU). ------- COMMENT: db9b5a7b5f36c25c 34 2ByX7wF/92sAQDUkuMa3MBgrCp8 (comment: VW, I am not sure that I captured this correctly?) A ChIP assay revealed that the association of Rec8(TEV) with chromatin is partly, but not entirely, impaired only at the centromeric central core region when cen-TEV protease is coexpressed (Figure 7C), suggesting that Rec8(TEV) is cleaved in a region-specific manner. We reasoned that, even if central core Rec8 is cleaved by cen-TEV protease, newly produced or free Rec8 complexes can be reloaded, resulting in the observed association of low levels of Rec8 at the central core. Nevertheless, such ‘‘turnover’’ of cohesin complexes would eventually abolish cohesion because newly associated cohesins do not reestablish cohesion after DNA replication. ------- COMMENT: dbb001257ca2e7c4 16 B30GSSRlhGgsC2TXdYlxcB/J/ds (comment: evidence is essentially IC, as I inferred sterility from the lack of shmoo formation (h- cells)) ------- COMMENT: dbb1e62e1d85e3b7 6 /LSBeYfLEjfEN6n7ah/sO2IXVZ8 (comment: CONDITION non-ionic osmotic stress) ------- COMMENT: dbb1e62e1d85e3b7 8 5d4EJ3/XXAYJehrdv2wUgNbbhzc (comment: CHECK salt stress) ------- COMMENT: dbb1e62e1d85e3b7 45 5d4EJ3/XXAYJehrdv2wUgNbbhzc (comment: CHECK salt stress) ------- COMMENT: dbcc669ae67615f0 1 CcAK+IFlkKcoU0LNcgj+njTcY5M Chromatin immunoprecipitation of this protein is highly enriched for centromeric sequences. ------- COMMENT: dbcc669ae67615f0 6 CcAK+IFlkKcoU0LNcgj+njTcY5M Chromatin immunoprecipitation of this protein is highly enriched for centromeric sequences. ------- COMMENT: dbcc669ae67615f0 7 CcAK+IFlkKcoU0LNcgj+njTcY5M Chromatin immunoprecipitation of this protein is highly enriched for centromeric sequences. ------- COMMENT: dbcc669ae67615f0 10 iw//icT6j0p+dJLkZY9c5Tfz7+w Chromatin immunoprecipitation of this protein is highly enriched in centromeric DNA ------- COMMENT: dbcc669ae67615f0 11 iw//icT6j0p+dJLkZY9c5Tfz7+w Chromatin immunoprecipitation of this protein is highly enriched in centromeric DNA ------- COMMENT: dbcc669ae67615f0 12 CcAK+IFlkKcoU0LNcgj+njTcY5M Chromatin immunoprecipitation of this protein is highly enriched for centromeric sequences. ------- COMMENT: dbe08e175eeaa405 1 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: dbe08e175eeaa405 2 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: dbe08e175eeaa405 4 vlVVCkH7Zax8H+iRWNfuCCAtXAM (comment: actually accumulation) ------- COMMENT: dc43fea8ff20c487 59 UdlTGE9lHvcVuChkceKGVPLygCA (comment: CHECK lys3 ura1) ------- COMMENT: dc43fea8ff20c487 63 UdlTGE9lHvcVuChkceKGVPLygCA (comment: CHECK lys3 ura1) ------- COMMENT: dc43fea8ff20c487 64 Tt2ZrI1gjtJN+8hdNa6zWyuFhLo (comment: CHECK ura1 met5) ------- COMMENT: dc43fea8ff20c487 65 XookDZOH8I7hR/4cscAaKX9b3u4 (comment: CHECK ade6 arg1) ------- COMMENT: dc43fea8ff20c487 67 UdlTGE9lHvcVuChkceKGVPLygCA (comment: CHECK lys3 ura1) ------- COMMENT: dc43fea8ff20c487 68 Tt2ZrI1gjtJN+8hdNa6zWyuFhLo (comment: CHECK ura1 met5) ------- COMMENT: dc43fea8ff20c487 72 UdlTGE9lHvcVuChkceKGVPLygCA (comment: CHECK lys3 ura1) ------- COMMENT: dc43fea8ff20c487 73 Tt2ZrI1gjtJN+8hdNa6zWyuFhLo (comment: CHECK ura1 met5) ------- COMMENT: dc43fea8ff20c487 78 UdlTGE9lHvcVuChkceKGVPLygCA (comment: CHECK lys3 ura1) ------- COMMENT: dc43fea8ff20c487 79 Tt2ZrI1gjtJN+8hdNa6zWyuFhLo (comment: CHECK ura1 met5) ------- COMMENT: dc43fea8ff20c487 82 UdlTGE9lHvcVuChkceKGVPLygCA (comment: CHECK lys3 ura1) ------- COMMENT: dc43fea8ff20c487 83 Tt2ZrI1gjtJN+8hdNa6zWyuFhLo (comment: CHECK ura1 met5) ------- COMMENT: dc5b0f7869ec439e 1 T0yyXx0m83/ddI4CkvAxZnCBdLg (comment: casein substrate) ------- COMMENT: dc5b0f7869ec439e 18 6gdIJKlVDzEA5VVo4jtlqxZHpZI (comment: casein substrate (vw changed from GO:0004674 with contributes to)) ------- COMMENT: dc8ce5293f6f8c29 1 77BX97Fy/WpQshPpHe6LX4ohkq4 (Fig. 1A and B) ------- COMMENT: dc8ce5293f6f8c29 2 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: dc8ce5293f6f8c29 3 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: dc8ce5293f6f8c29 4 p3x2dDTjQlS9jbO+IMHpHbED4nw (Fig. 1D) ------- COMMENT: dc8ce5293f6f8c29 5 77BX97Fy/WpQshPpHe6LX4ohkq4 (Fig. 1A and B) ------- COMMENT: dc8ce5293f6f8c29 6 dwqMs7wxPLJsoccjT/9Y78e2sLw (Fig. 1A and C) ------- COMMENT: dc8ce5293f6f8c29 7 dwqMs7wxPLJsoccjT/9Y78e2sLw (Fig. 1A and C) ------- COMMENT: dc8ce5293f6f8c29 8 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: dc8ce5293f6f8c29 9 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: dc8ce5293f6f8c29 10 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: dc8ce5293f6f8c29 11 p3x2dDTjQlS9jbO+IMHpHbED4nw (Fig. 1D) ------- COMMENT: dc8ce5293f6f8c29 12 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: dc8ce5293f6f8c29 13 p3x2dDTjQlS9jbO+IMHpHbED4nw (Fig. 1D) ------- COMMENT: dc8ce5293f6f8c29 14 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: dc8ce5293f6f8c29 15 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: dc8ce5293f6f8c29 16 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: dc8ce5293f6f8c29 17 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: dc8ce5293f6f8c29 18 cgUZ9Rgpi1C57s7n5arqT9ZUWoE (Fig. 2B and C) ------- COMMENT: dc8ce5293f6f8c29 19 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: dc8ce5293f6f8c29 20 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: dc8ce5293f6f8c29 21 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: dc8ce5293f6f8c29 22 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: dc8ce5293f6f8c29 23 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: dc8ce5293f6f8c29 24 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: dc8ce5293f6f8c29 25 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: dc8ce5293f6f8c29 26 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: dc8ce5293f6f8c29 27 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: dc8ce5293f6f8c29 28 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: dc8ce5293f6f8c29 29 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: dc8ce5293f6f8c29 30 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: dc8ce5293f6f8c29 31 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: dc8ce5293f6f8c29 32 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: dc8ce5293f6f8c29 33 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: dc8ce5293f6f8c29 34 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: dc8ce5293f6f8c29 35 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: dc8ce5293f6f8c29 36 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: dc8ce5293f6f8c29 37 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: dc8ce5293f6f8c29 38 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: dc8ce5293f6f8c29 39 BizWtp5a3FuRCOPlcVHe5Y8cdfA (Fig. 4A, 4B, Fig. S5B) ------- COMMENT: dc8ce5293f6f8c29 42 lzDpBhI+e1KR8hAG7s00IgdEh2I (Fig. 5A) ------- COMMENT: dc8ce5293f6f8c29 43 lzDpBhI+e1KR8hAG7s00IgdEh2I (Fig. 5A) ------- COMMENT: dc8ce5293f6f8c29 44 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: dc8ce5293f6f8c29 45 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: dc8ce5293f6f8c29 46 hPFNK9pXn9s6uyKJPTkg3DIM2XE (Fig. 6A) ------- COMMENT: dc8ce5293f6f8c29 47 hPFNK9pXn9s6uyKJPTkg3DIM2XE (Fig. 6A) ------- COMMENT: dc8ce5293f6f8c29 48 hPFNK9pXn9s6uyKJPTkg3DIM2XE (Fig. 6A) ------- COMMENT: dc8ce5293f6f8c29 49 E9ksZLZljr6NCHOE+Y+HtOZlWxo (Fig. 7B, C and D) ------- COMMENT: dc8ce5293f6f8c29 50 nnOxmYl/Vxfpx3AN26h4Xe+UFAA (Fig. 7A, C, D and E) ------- COMMENT: dc8ce5293f6f8c29 51 YSfvpxZxXYWLkz1N2bWoK/o2ZgM (Fig. 7F and G) ------- COMMENT: dc8ce5293f6f8c29 52 YSfvpxZxXYWLkz1N2bWoK/o2ZgM (Fig. 7F and G) ------- COMMENT: dc8ce5293f6f8c29 53 YSfvpxZxXYWLkz1N2bWoK/o2ZgM (Fig. 7F and G) ------- COMMENT: dc8ce5293f6f8c29 54 YSfvpxZxXYWLkz1N2bWoK/o2ZgM (Fig. 7F and G) ------- COMMENT: dc8ce5293f6f8c29 55 VDgFIXLreAO01Uq9+wAGAIFQQt4 (Fig. 6C, D and E) ------- COMMENT: dc8ce5293f6f8c29 56 sUCS79Rs9xFD1wViTRlsgeGdNFs (Fig. 6B) ------- COMMENT: dc8ce5293f6f8c29 57 9hRZH5OVi638qJERrLIHfdnrEM4 We show that Bgs1-synthetized linear b(1,3)glucan cooperates specifically with the Tea1-Tea4 complex, but not with the rest of polarisome proteins, and all together are essential for the control and maintenance of growth polarity and morphology (Figure 5C). ------- COMMENT: dc8fa114b5d1cd1f 1 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: dc8fa114b5d1cd1f 2 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: dc8fa114b5d1cd1f 3 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: dc8fa114b5d1cd1f 4 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: dc8fa114b5d1cd1f 5 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: dc8fa114b5d1cd1f 6 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: dc8fa114b5d1cd1f 7 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: dc8fa114b5d1cd1f 8 DF1L9AR2+LxgyOMYptBBoA5zAsw Nuclear displacement in cut7-22 was rescued by cdc12-112 ------- COMMENT: dc8fa114b5d1cd1f 9 hIDSV5MD+Js0PuL6EOle6OWcNvU Nuclear displacement in cut7-22 was rescued by rng3-65 ------- COMMENT: dc8fa114b5d1cd1f 10 ABLKEQmsVNw87xFFUZJkMOO6Vy4 Nuclear displacement in cut7-22 was rescued by myo2-E1 myo51Δ ------- COMMENT: dc8fa114b5d1cd1f 11 ABLKEQmsVNw87xFFUZJkMOO6Vy4 Nuclear displacement in cut7-22 was rescued by myo2-E1 myo51Δ ------- COMMENT: dc8fa114b5d1cd1f 12 YEAjGwhFpYK9DFnQuADADbgKvcc Nuclear displacement in cut7-22 was partially rescued by cdc7-24 ------- COMMENT: dc8fa114b5d1cd1f 13 TEiuPVbdaJG8xGEUYbIXUlVxh+U Nuclear displacement in cut7-22 was partially rescued by mad2Δ ------- COMMENT: dc8fa114b5d1cd1f 14 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: dc8fa114b5d1cd1f 15 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: dc8fa114b5d1cd1f 16 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: dc8fa114b5d1cd1f 17 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: dc8fa114b5d1cd1f 18 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: dc8fa114b5d1cd1f 19 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: dc8fa114b5d1cd1f 20 EdexklW1t8NlDIPrcwLP1cKj0+A fig2 (comment: live cell imaging) ------- COMMENT: dc8fa114b5d1cd1f 21 EdexklW1t8NlDIPrcwLP1cKj0+A fig2 (comment: live cell imaging) ------- COMMENT: dc8fa114b5d1cd1f 22 Sw131tbg02r5Kn//2VAn2QLOiP8 Fig 7 These results indicate that first, the main reason for lethality of cut7 is derived from the cut phenotype; second, some cells could escape from cut by displacing the nucleus from the middle of the cell axis; and finally, these cut7 survivors could resume cell division as diploid progenies at the permissive temperature. ------- COMMENT: dc8fa114b5d1cd1f 23 wreww1NjNj+qfxF5yDLCCyEIGLA fig4 (comment: If possible, please add the following comment - “The nucleus is retained in the center of the cell during mitosis.") ------- COMMENT: dc8fa114b5d1cd1f 24 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: dc8fa114b5d1cd1f 25 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: dc8fa114b5d1cd1f 26 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: dc8fa114b5d1cd1f 27 1HG7HkAze7m1hZX5NVXtHu9jutE Fig7 ------- COMMENT: dcbc3aa3fb96acba 15 TUjyQf0BwbKLg7nF/X549Hnriao Consistent with this assumption, we found that dnt1Δ cells lost minichromosomes (Ch16, ade6-M216) at an elevated rate that is almost 100 times higher than that of the wild-type (Fig 1B), and displayed increased frequency of lagging chromosomes and chromosome mis-segre- gation at mitotic anaphase (Fig 1C). ------- COMMENT: dcbc3aa3fb96acba 17 11rfYwEa21f38PI1el9MIWpd0sg inefficient anaphase initiation upon SAC inactivation/ persistent MCC-APC/C binding upon SAC activation The SAC was first robustly activated by the nda3-KM311 mutant then inactivated by shifting mitotically arrested cells back to permissive temperature(30˚C)/ We found that dnt1Δ cells retained high amounts of SPB-localized Cdc13-GFP and nuclear Cut2-GFP for much pro- longed period compared to wild-type cells, almost to the same degree as previously identified SAC-inactivation defective mutant dis2Δ ------- COMMENT: dcbc3aa3fb96acba 21 CXwIWWWJePDDVV6RaQkn30F8XEY (comment: I changed to decreased. becasue the phenotype is compared to WT,) ------- COMMENT: dcbc3aa3fb96acba 22 xDRXCBQWgCUVaFrvIFPm7vz06zs Surprisingly, Slp1Cdc20 was slightly, but appreciably and reproducibly, less abundant (ranging from roughly 20% to 50% at different time points) in dnt1Δ cells than in wild-type cells (Fig 3B), suggesting Dnt1 may indeed positively regulate the levels of intact Slp1Cdc20. In addition, this regulation of Slp1Cdc20 stability by Dnt1 is Dma1-independent, as the dnt1Δ dma1Δ double mutant has a similar level and degradation profile of Slp1Cdc20 as dnt1Δ single mutant (S5 Fig). ------- COMMENT: dcbc3aa3fb96acba 23 DCzAP4NwoF5ppzpfx0/GEa3PzIQ (comment: upon SAC activation) ------- COMMENT: dcbc3aa3fb96acba 24 DCzAP4NwoF5ppzpfx0/GEa3PzIQ (comment: upon SAC activation) ------- COMMENT: dcbc3aa3fb96acba 25 kkkKvXjj9IUDUp1KaGrwNIHlh+4 but dnt1Δ cells stayed for extended length of time at ana- phase B (Fig 1D–1F) ------- COMMENT: dcbc3aa3fb96acba 38 w8IzFmGbeYkYLHdC2hZFiCOpKYY but dnt1Δ cells stayed for extended length of time at anaphase B (Fig 1D–1F) ------- COMMENT: dcbc3aa3fb96acba 41 TfbNa+VEbMbQ5b14VKgQ8/C4REQ Consequently, sensitivity of dnt1Δ cells to TBZ was largely but not completely suppressed by excessive Slp1Cdc20 expression achieved by three copies of slp1+ (Fig 3E). ------- COMMENT: dcd04bfe23f43146 104 9ktr2xnYkIchnmVb2v3vyNEndlk in fig s4b there is septal material hanging around one cell end ------- COMMENT: dcd04bfe23f43146 106 lQNUDZiN63r5cHi22olkqtUB8xE Fig. S5A ------- COMMENT: dcdf06bafb5cb58c 1 tdY+sTkcVXQXSK7tyg52iDlFg+Y (Fig. S1A) ------- COMMENT: dcdf06bafb5cb58c 2 B+51PdZSwSquo7jRIKWKTvEByG4 (Fig. S2E) ------- COMMENT: dcdf06bafb5cb58c 3 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: dcdf06bafb5cb58c 4 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: dcdf06bafb5cb58c 5 L84RMYxcmJK2Zj7t0R82RKoWDok (Fig. S6C) ------- COMMENT: dcdf06bafb5cb58c 6 V1iAQoqm4heXcblKCi7+V8yd0mg (Fig. S6D) ------- COMMENT: dcdf06bafb5cb58c 7 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: dcdf06bafb5cb58c 8 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: dcdf06bafb5cb58c 9 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: dcdf06bafb5cb58c 10 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: dcdf06bafb5cb58c 11 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: dcdf06bafb5cb58c 12 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: dcdf06bafb5cb58c 13 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: dcdf06bafb5cb58c 14 wnx2gS3IAKqzPkStlyJ0uF9GveM (Fig. 6C) ------- COMMENT: dcdf06bafb5cb58c 15 LWu2FtVw4TGF2Mzg8ysWg92c+38 (Fig. S6E) ------- COMMENT: dcdf06bafb5cb58c 16 XwjFKIBpFnAfCL+3uv8ZHt+X4Cc (Fig. 6D) ------- COMMENT: dcdf06bafb5cb58c 17 mHHK047Ybq4WyRgqcXmo6GlF13I (Fig. S6F) ------- COMMENT: dcdf06bafb5cb58c 18 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: dcdf06bafb5cb58c 22 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: dcdf06bafb5cb58c 23 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: dcdf06bafb5cb58c 24 dSuM4+IyDolwEuKsWneeLfc4zg4 (Fig. 1B and C) ------- COMMENT: dcdf06bafb5cb58c 25 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: dcdf06bafb5cb58c 26 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: dcdf06bafb5cb58c 27 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: dcdf06bafb5cb58c 29 gZNo/xWKvKsHWWGOazkQEqseH2U (Fig. 1) ------- COMMENT: dcdf06bafb5cb58c 36 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 37 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 38 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 39 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 40 ojGoCV7tkFuQq8EQLxws0hw4rK4 (Fig. S2D) ------- COMMENT: dcdf06bafb5cb58c 41 ojGoCV7tkFuQq8EQLxws0hw4rK4 (Fig. S2D) ------- COMMENT: dcdf06bafb5cb58c 42 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: dcdf06bafb5cb58c 43 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: dcdf06bafb5cb58c 44 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: dcdf06bafb5cb58c 45 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: dcdf06bafb5cb58c 46 SyRhWZVZy+NSyjVFp7p2obx1YKw (Fig. 3A and C) ------- COMMENT: dcdf06bafb5cb58c 47 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: dcdf06bafb5cb58c 48 gRK0bumYnCVPmKXmgeHPXg/LLNc (Fig. S3C) ------- COMMENT: dcdf06bafb5cb58c 49 6VsstCmAvdpmDgIHvwkAQkedba4 (Fig. 4B, D and E) ------- COMMENT: dcdf06bafb5cb58c 50 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 51 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 52 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 53 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 54 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: dcdf06bafb5cb58c 55 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: dcdf06bafb5cb58c 56 SeRjS20UIgqJCoVGGb5GUMXIMf0 (Fig. S5F) ------- COMMENT: dcdf06bafb5cb58c 59 GkI93EYSqP6NybAtsJjxO2fy3Ic (Fig. 4D) ------- COMMENT: dcdf06bafb5cb58c 60 NuNHLmtoq4GzF6sDw6D9LwteRCo (Fig. S4B) ------- COMMENT: dcdf06bafb5cb58c 61 NuNHLmtoq4GzF6sDw6D9LwteRCo (Fig. S4B) ------- COMMENT: dcdf06bafb5cb58c 62 GkI93EYSqP6NybAtsJjxO2fy3Ic (Fig. 4D) ------- COMMENT: dcdf06bafb5cb58c 63 NuNHLmtoq4GzF6sDw6D9LwteRCo (Fig. S4B) ------- COMMENT: dcdf06bafb5cb58c 64 GkI93EYSqP6NybAtsJjxO2fy3Ic (Fig. 4D) ------- COMMENT: dcdf06bafb5cb58c 66 NuNHLmtoq4GzF6sDw6D9LwteRCo (Fig. S4B) ------- COMMENT: dcdf06bafb5cb58c 67 GkI93EYSqP6NybAtsJjxO2fy3Ic (Fig. 4D) ------- COMMENT: dcdf06bafb5cb58c 68 HbxAPepjRn9GH0creuUodcs/d7c (Fig. S4F) ------- COMMENT: dcdf06bafb5cb58c 69 HQx/nKU+Np0MQgZtcp2gru69Zq0 (Fig. S4E) ------- COMMENT: dcdf06bafb5cb58c 70 j30KwK8QbZkLhoky9eRwFbLzgAY (Fig. S4C) ------- COMMENT: dcdf06bafb5cb58c 71 B+51PdZSwSquo7jRIKWKTvEByG4 (Fig. S2E) ------- COMMENT: dcdf06bafb5cb58c 72 B+51PdZSwSquo7jRIKWKTvEByG4 (Fig. S2E) ------- COMMENT: dcdf06bafb5cb58c 73 tdY+sTkcVXQXSK7tyg52iDlFg+Y (Fig. S1A) ------- COMMENT: dcdf06bafb5cb58c 74 tdY+sTkcVXQXSK7tyg52iDlFg+Y (Fig. S1A) ------- COMMENT: dcdf06bafb5cb58c 75 wnx2gS3IAKqzPkStlyJ0uF9GveM (Fig. 6C) ------- COMMENT: dcdf06bafb5cb58c 76 XwjFKIBpFnAfCL+3uv8ZHt+X4Cc (Fig. 6D) ------- COMMENT: dcdf06bafb5cb58c 77 SyRhWZVZy+NSyjVFp7p2obx1YKw (Fig. 3A, 3C) ------- COMMENT: dcdf06bafb5cb58c 78 MfhXrratyk9HEpPvYNKyPqJLEoI (Fig. 4E) ------- COMMENT: dcdf06bafb5cb58c 79 UiG5r5ufQlxFfkfkP/H+hJFW9Sk (Fig. S3E) ------- COMMENT: dcdf06bafb5cb58c 80 DVJMM6AvJyLz8J8dkwtY+pFizxc (Fig. 5E) ------- COMMENT: dcdf06bafb5cb58c 81 DVJMM6AvJyLz8J8dkwtY+pFizxc (Fig. 5E) ------- COMMENT: dcdf06bafb5cb58c 82 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 83 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 84 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 85 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 86 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 87 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 88 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 89 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 90 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: dcdf06bafb5cb58c 91 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: dcdf06bafb5cb58c 92 gRK0bumYnCVPmKXmgeHPXg/LLNc (Fig. S3C) ------- COMMENT: dcdf06bafb5cb58c 93 UiG5r5ufQlxFfkfkP/H+hJFW9Sk (Fig. S3E) ------- COMMENT: dcdf06bafb5cb58c 94 4CJ6vx8Kjku2u9dbK5ZoujERcjU (Fig. S3F) ------- COMMENT: dcdf06bafb5cb58c 95 MfhXrratyk9HEpPvYNKyPqJLEoI (Fig. 4E) ------- COMMENT: dcdf06bafb5cb58c 96 XVHahgPXZsFOyduY4BP8CoHD6gY (Fig. 5E, 5F) ------- COMMENT: dcdf06bafb5cb58c 97 XVHahgPXZsFOyduY4BP8CoHD6gY (Fig. 5E, 5F) ------- COMMENT: dcdf06bafb5cb58c 98 9obkRBahnsvfuKPq+LTlya+asC4 (Fig. 6E) ------- COMMENT: dcdf06bafb5cb58c 99 wqgJ/LsQVMsNVTLzO9M+qdDHyXY (Fig. 6F) ------- COMMENT: dcdf06bafb5cb58c 100 kIhmBd7G1n6/01SpGv20pzgvebY (Fig. 6G) ------- COMMENT: dcdf06bafb5cb58c 101 XAsbCR5NLjCj4dGhA32wFD0xglQ (Fig. 6H) ------- COMMENT: dcdf06bafb5cb58c 102 4CJ6vx8Kjku2u9dbK5ZoujERcjU (Fig. S3F) ------- COMMENT: dcdf06bafb5cb58c 103 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 104 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 105 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 106 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 107 UiG5r5ufQlxFfkfkP/H+hJFW9Sk (Fig. S3E) ------- COMMENT: dcdf06bafb5cb58c 108 MfhXrratyk9HEpPvYNKyPqJLEoI (Fig. 4E) ------- COMMENT: dcdf06bafb5cb58c 110 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: dcdf06bafb5cb58c 111 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: dcdf06bafb5cb58c 112 gRK0bumYnCVPmKXmgeHPXg/LLNc (Fig. S3C) ------- COMMENT: dcdf06bafb5cb58c 113 UiG5r5ufQlxFfkfkP/H+hJFW9Sk (Fig. S3E) ------- COMMENT: dcdf06bafb5cb58c 114 4CJ6vx8Kjku2u9dbK5ZoujERcjU (Fig. S3F) ------- COMMENT: dcdf06bafb5cb58c 115 MfhXrratyk9HEpPvYNKyPqJLEoI (Fig. 4E) ------- COMMENT: dcdf06bafb5cb58c 116 qgy2UtS49Rc8vkPrVMir2qiYd3M (Fig. 5F) ------- COMMENT: dcdf06bafb5cb58c 117 DVJMM6AvJyLz8J8dkwtY+pFizxc (Fig. 5E) ------- COMMENT: dcdf06bafb5cb58c 118 9obkRBahnsvfuKPq+LTlya+asC4 (Fig. 6E) ------- COMMENT: dcdf06bafb5cb58c 119 wqgJ/LsQVMsNVTLzO9M+qdDHyXY (Fig. 6F) ------- COMMENT: dcdf06bafb5cb58c 120 kIhmBd7G1n6/01SpGv20pzgvebY (Fig. 6G) ------- COMMENT: dcdf06bafb5cb58c 121 XAsbCR5NLjCj4dGhA32wFD0xglQ (Fig. 6H) ------- COMMENT: dcdf06bafb5cb58c 122 SamTvRZvEvQIrgSzuqc8al8jR1s (Fig. S6B) ------- COMMENT: dcdf06bafb5cb58c 123 SamTvRZvEvQIrgSzuqc8al8jR1s (Fig. S6B) ------- COMMENT: dcdf06bafb5cb58c 124 BlZAYgg6W/+/O+S+HMuoElV4g98 (Fig. S6A) ------- COMMENT: dcdf06bafb5cb58c 125 L84RMYxcmJK2Zj7t0R82RKoWDok (Fig. S6C) ------- COMMENT: dcdf06bafb5cb58c 126 V1iAQoqm4heXcblKCi7+V8yd0mg (Fig. S6D) ------- COMMENT: dcdf06bafb5cb58c 127 L84RMYxcmJK2Zj7t0R82RKoWDok (Fig. S6C) ------- COMMENT: dcdf06bafb5cb58c 128 V1iAQoqm4heXcblKCi7+V8yd0mg (Fig. S6D) ------- COMMENT: dcdf06bafb5cb58c 129 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 130 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 131 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 132 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 133 c7jK/W7G++NtcMzBtsQ+SsC3gh8 5’&3’SSmut cells also exhibited a strong increase in signal intensity and a redistribution of the lncRNA to the nucleolus, as determined by fluorescence overlap with the nucleolar marker Nop56 (Fig. 1d, e) ------- COMMENT: dcdf06bafb5cb58c 134 dSuM4+IyDolwEuKsWneeLfc4zg4 (Fig. 1B, 1C) ------- COMMENT: dcdf06bafb5cb58c 135 x6bYail8zpFRBS2WbizW7loub2A (Fig. 3D) ------- COMMENT: dcdf06bafb5cb58c 136 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: dcdf06bafb5cb58c 137 5o1JnSr8/P6LFOlJ5ONpz6ENKwo (Fig. 1, Fig. 2D) ------- COMMENT: dcdf06bafb5cb58c 138 SyRhWZVZy+NSyjVFp7p2obx1YKw (Fig. 3A, 3C) ------- COMMENT: dcdf06bafb5cb58c 139 GkI93EYSqP6NybAtsJjxO2fy3Ic (Fig. 4D) ------- COMMENT: dcdf06bafb5cb58c 140 SeRjS20UIgqJCoVGGb5GUMXIMf0 (Fig. S5F) ------- COMMENT: dcdf06bafb5cb58c 141 NuNHLmtoq4GzF6sDw6D9LwteRCo (Fig. S4B) ------- COMMENT: dcdf06bafb5cb58c 142 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: dcdf06bafb5cb58c 143 gRK0bumYnCVPmKXmgeHPXg/LLNc (Fig. S3C) ------- COMMENT: dcdf06bafb5cb58c 144 /Iubo4If9ouXhvCNz5P0NF8X3AQ We further carried out smFISH analyses and found that snR107, as opposed to mamRNA 5’ exon, accumulated specifically in the nucleolus in wild type cells (Fig. 2d, e). ------- COMMENT: dcdf06bafb5cb58c 145 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: dcdf06bafb5cb58c 146 djYgMxdoFiNXCq97x0rHLJiSASE (Fig. 3C) ------- COMMENT: dcdf06bafb5cb58c 147 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 148 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 149 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 150 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: dcdf06bafb5cb58c 151 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: dcdf06bafb5cb58c 152 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: dcdf06bafb5cb58c 153 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: dcdf06bafb5cb58c 154 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: dcdf06bafb5cb58c 155 LWu2FtVw4TGF2Mzg8ysWg92c+38 (Fig. S6E) ------- COMMENT: dcdf06bafb5cb58c 156 mHHK047Ybq4WyRgqcXmo6GlF13I (Fig. S6F) ------- COMMENT: dcdf06bafb5cb58c 157 bVkLbvkED/e2N/w+Sqbzfpr5oTc (Fig. S3G) ------- COMMENT: dcdf06bafb5cb58c 158 bVkLbvkED/e2N/w+Sqbzfpr5oTc (Fig. S3G) ------- COMMENT: dcdf06bafb5cb58c 159 bVkLbvkED/e2N/w+Sqbzfpr5oTc (Fig. S3G) ------- COMMENT: dcdf06bafb5cb58c 160 bVkLbvkED/e2N/w+Sqbzfpr5oTc (Fig. S3G) ------- COMMENT: dcdf06bafb5cb58c 161 bVkLbvkED/e2N/w+Sqbzfpr5oTc (Fig. S3G) ------- COMMENT: dcdf06bafb5cb58c 162 1vITJkX365/kSO7rGYqR9cXiOZI (Fig. S4D) ------- COMMENT: dcdf06bafb5cb58c 163 PEijj9MIrNSBSecC4MlXqO1v4Zk (Fig. 3) ------- COMMENT: dcdf06bafb5cb58c 164 VKLy+IxIj2P1d4IT18rLQymnI60 From these experiments, we concluded that snR107 mediates 25S rRNA G2483 2’-O-Me and contributes to pre-rRNA processing and 60S subunit biogenesis in a Gm2483-independent fashion. ------- COMMENT: dd06290a49093559 11 UfuJ4U8DbBJYAwY898avt2rUBQQ (comment: chromatin recruiter) Together, we concluded that the primary defects observed in the ndc80-AK01 mutant can be attributed to impaired Mph1 recruitment to kinetochores, which leads to failure in recruitment of the other SAC components and abortive mitotic arrest. (comment: COUld also get 'upstream of/affects SAC") ------- COMMENT: dd0b314b0bd84119 2 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: dd0b314b0bd84119 3 T3SIS/qo8KjQn8PZQ9cVIdU7D0k fig 1d, 1k ------- COMMENT: dd0b314b0bd84119 4 02l+3cqTh7UTFJSYjyKhzJabH68 fig1c ------- COMMENT: dd0b314b0bd84119 5 +Ui5FUm6tm7Jtzjd3RNBnhJxqOQ The cdc13-M7 mutant is suppressed by bir1-8D ------- COMMENT: dd0b314b0bd84119 6 2iJo+Jp/wFV4Ls6GwAbMGE2jlkQ fig 1k ------- COMMENT: dd0b314b0bd84119 7 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: dd0b314b0bd84119 8 wb8+3kAgYc7JGuHj/SRKrzp4b3M (comment: CHECK figb) ------- COMMENT: dd0b314b0bd84119 10 P/N8BhvFWNlzEGOxmvC3f3ET1SU fig 1b ------- COMMENT: dd0b314b0bd84119 11 mHlQNfnsgD+vcX6287k6udxbURY fig S1. Nuclear staining of anaphase cells showed that the cdc13-M7 mutant, but not the conventional cdc13-117 mutant, often exhibited lagging chromosomes at anaphase (Fig. 1c). ------- COMMENT: dd0b314b0bd84119 13 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: dd0b314b0bd84119 14 XuKEoN5RtkeKSfEy8vtyYvrsgYM Fig. 1e, Fig. 1f ------- COMMENT: dd0b314b0bd84119 15 8NFW+NKZwYGzq0dLa8ucWpqHtzw Fig. 1e also The in vivo phosphorylation of Bir1 at prometaphase but not interphase was further confirmed by a phospho-specific antibody against one of the CDK sites, Bir1-pS244 (Supplementary Fig. 2). ------- COMMENT: dd0b314b0bd84119 16 YFyOOtey07hcj+yJNrBxoxdWY3U Fig 1h ------- COMMENT: dd0b314b0bd84119 17 IjVFFarVrcDYU5swPkVP05h696M Fig 1i ------- COMMENT: dd0b314b0bd84119 18 IruOv+/R586ggIcxQTv2D5AZTxA fig1j ------- COMMENT: dd0b314b0bd84119 19 IruOv+/R586ggIcxQTv2D5AZTxA fig1j ------- COMMENT: dd0b314b0bd84119 23 QSfoQyo9WTR4mNvS84Fa2jmXXLw fig1S4, figS5 ------- COMMENT: dd0b314b0bd84119 24 +6ooEyPOYHOcdEUgPcaC5SERWqM fig2a, 2b, figS5 ------- COMMENT: dd0b314b0bd84119 25 j8lx0cjXOogtvtAy6trm2XWxJ9Q fig2a ------- COMMENT: dd0b314b0bd84119 26 j8lx0cjXOogtvtAy6trm2XWxJ9Q fig2a ------- COMMENT: dd0b314b0bd84119 27 a1gYpwUP4TlenEFAgWwQpczNLvE figS6 ------- COMMENT: dd0b314b0bd84119 28 a1gYpwUP4TlenEFAgWwQpczNLvE figS6 ------- COMMENT: dd0b314b0bd84119 32 auyglAIihsOiF7/23PQxdIPA1C8 suppressed at comparable level to Bir1–CD, These results indicate that once they are tethered at centromeres, the functionality is indistinguishable between Bir1 and Bir1-8A. Supporting this conclusion, complex formation of the CPC was intact in bir1-8A cells (Supplementary Fig. 7). ------- COMMENT: dd0b314b0bd84119 33 jR5Xbco450w6eB6i9nKUfd5UbRE Supplementary Fig. 8a) ------- COMMENT: dd0b314b0bd84119 34 1eQTMBH/kj0hfPzJ2MLz+VXGEwI Bir1-N-5A abolished the interaction with Sgo2, whereas Bir1-N-5D retained the interaction (Fig. 2h) ------- COMMENT: dd0b314b0bd84119 35 1eQTMBH/kj0hfPzJ2MLz+VXGEwI Bir1-N-5A abolished the interaction with Sgo2, whereas Bir1-N-5D retained the interaction (Fig. 2h) ------- COMMENT: dd0b314b0bd84119 36 +6ooEyPOYHOcdEUgPcaC5SERWqM fig2a, 2b, figS5 ------- COMMENT: dd0b314b0bd84119 37 +6ooEyPOYHOcdEUgPcaC5SERWqM fig2a, 2b, figS5 ------- COMMENT: dd0b314b0bd84119 38 jR5Xbco450w6eB6i9nKUfd5UbRE Supplementary Fig. 8a) ------- COMMENT: dd0dc92db12b47e2 24 dHHh70pG9e1TqNeAWCr9tvuNCAQ (comment: CHECK during anaphase) ------- COMMENT: dd0dc92db12b47e2 25 qNjf4TmRKFetg7AUFIpfHY5AwQE (comment: CHECK delayed during anaphase) ------- COMMENT: dd0dc92db12b47e2 28 UoaqbBNphGp5a7B3vWv43Gmlz34 (comment: total protein in proteasome mutant) ------- COMMENT: dd0dc92db12b47e2 29 UoaqbBNphGp5a7B3vWv43Gmlz34 (comment: total protein in proteasome mutant) ------- COMMENT: dd0dc92db12b47e2 31 IlK6nN2JCIFdurbDVMTzoHddf9c (comment: CHECK during G1 arrest ) fig4C right hand panel ------- COMMENT: dd0dc92db12b47e2 32 IlK6nN2JCIFdurbDVMTzoHddf9c (comment: CHECK during G1 arrest ) fig4C right hand panel ------- COMMENT: dd0dc92db12b47e2 33 QxQ3mnd33iXNoSWR/ecG8unnjco (comment: total ubiquitinated) ------- COMMENT: dd0dc92db12b47e2 34 LacL/MYMMqF0ONWZeSKcGZediuA (comment: ubiquitinated) ------- COMMENT: dd0dc92db12b47e2 35 LacL/MYMMqF0ONWZeSKcGZediuA (comment: ubiquitinated) ------- COMMENT: dd3170869113df7a 1 c3Vll+UD1Qasno0ETWWKSPXduLk Spo13 interacted with both GTP- and GDP-bound forms of Ypt3 (Figure 7, Supplemental Figure S8). ------- COMMENT: dd3170869113df7a 2 c3Vll+UD1Qasno0ETWWKSPXduLk Spo13 interacted with both GTP- and GDP-bound forms of Ypt3 (Figure 7, Supplemental Figure S8). ------- COMMENT: dd3170869113df7a 3 z7oHHJl9ms4NOVJQ7vRK1Qv4kpI (comment: CHECK ypt2-S18V (GDP-bound form)) (Supplemental Figure S7) ------- COMMENT: dd3170869113df7a 4 z7oHHJl9ms4NOVJQ7vRK1Qv4kpI (comment: CHECK ypt2-S18V (GDP-bound form)) (Supplemental Figure S7) ------- COMMENT: dd3170869113df7a 5 VNLGsYbevF3mgq+3UHPkMkzKy4A Figure 1, Supplemental Figure S1 ------- COMMENT: dd3170869113df7a 6 cg4Pkd4NSpmb+881a56inkVyCgg (comment: from metaphase II to postmeiosis) (Figure 2) ------- COMMENT: dd3170869113df7a 7 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: dd3170869113df7a 8 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: dd3170869113df7a 9 AUhQ051WCvbAFc+G9YgUfcRX048 Figure 1, Supplemental Figure S1 ------- COMMENT: dd3170869113df7a 10 H+GOVWqpOW2fXY7Kgap2lS4QfPc Sec2 interacted with Ypt3 in GDP-bound form (Figure 7). ------- COMMENT: dd3170869113df7a 11 Hr4OrJDPgLN/72iMWnEUlazcsQE (comment: CHECK in vegetative cells) (Supplemental Figure S10); ------- COMMENT: dd3170869113df7a 12 hmwmdLsKHzuSb//Dm+q1clz2tpI (comment: CHECK in sporulating cells) (Figure 9, Supplemental Figure S9, Supplemental Figure S10) ------- COMMENT: dd3170869113df7a 13 bqKeN7n62Z+nOTwrGXINf57vsRE (comment: CHECK during meiosis) (Figure 5, Supplemental Figure S4) ------- COMMENT: dd3170869113df7a 14 2Xk0qBIW+FJLEP1ul3cnx4YBu30 (comment: CHECK localizations at spindle poles during meiotic anaphase I) (Figure 6, Supplemental Figure S3) ------- COMMENT: dd3170869113df7a 15 xtsD2UJbMCt6Oy0Hk8hSMC4comU (comment: CHECK localizations at spindle poles during meiotic anaphase I) (Figure 6, Supplemental Figure S6) ------- COMMENT: dd3170869113df7a 16 1RMiaDpZf6/yPoKOcDLqFxZaOVo Figure 6, Supplemental Figure S6 ------- COMMENT: dd3170869113df7a 17 z981zqc9+mASfcJbHjWdXvpKny4 Supplemental Figure S11 ------- COMMENT: dd3170869113df7a 18 z981zqc9+mASfcJbHjWdXvpKny4 Supplemental Figure S11 ------- COMMENT: dd3170869113df7a 19 Q9Nu3F/JrgmnS0I+aF4y50/vrnc (Supplemental Figure S11) ------- COMMENT: dd3170869113df7a 20 HKX7gZsCutj05q6wCMLCXdCWJOM Figure 8, Supplemental Figure S9 ------- COMMENT: dd3170869113df7a 21 /6LsY5BmFsUCkuS5mxK2o1sjylo (comment: CHECK initiation of forespore membrane delayed )(Figure 3, Table 2) ------- COMMENT: dd3170869113df7a 22 8PRDD8mHIyKxLsyb6Sdw5HJKUW8 Figure 4, 5, Supplemental Figure S4 ------- COMMENT: dd3170869113df7a 23 e0wMZpJfzgtG2VC6l3Sm05D6zUk Figure 4, 5 ------- COMMENT: dd3170869113df7a 24 s3y19l7aG6aUsie7r1JA3QQCkss Supplemental Figure S12 ------- COMMENT: dd3170869113df7a 25 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: dd3170869113df7a 26 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: dd3170869113df7a 27 2bPIi8MvQroWIsFRkArH+gpyed8 Figure 5C, Supplemental Figure S4B ------- COMMENT: dd3170869113df7a 28 hjWhsSgOhESI6QMK+fbzgWouej4 Supplemental Figure S5A ------- COMMENT: dd3170869113df7a 29 SXlJR84BKjCOA56m8bgeLBQHOzA Figure 6A, Supplemental Figure S3) ------- COMMENT: dd3170869113df7a 30 hjWhsSgOhESI6QMK+fbzgWouej4 Supplemental Figure S5A ------- COMMENT: dd3170869113df7a 31 SXlJR84BKjCOA56m8bgeLBQHOzA Figure 6A, Supplemental Figure S3) ------- COMMENT: dd3170869113df7a 32 hjWhsSgOhESI6QMK+fbzgWouej4 Supplemental Figure S5A ------- COMMENT: dd3170869113df7a 33 xNz9yGqcjTTSpWAQmVzlgIdQW/g Sec2 interacted with specifically with GTP- bound forms of Ypt3 (Figure 7, Supplemental Figure S8). ------- COMMENT: dd3170869113df7a 34 GrSt3UMF5oH6iw4sU5Cd2G2Vdo4 (Figure 8A) ------- COMMENT: dd3170869113df7a 35 GrSt3UMF5oH6iw4sU5Cd2G2Vdo4 (Figure 8A) ------- COMMENT: dd3170869113df7a 36 GrSt3UMF5oH6iw4sU5Cd2G2Vdo4 (Figure 8A) ------- COMMENT: dd3170869113df7a 37 SEah3c1fAaTjHtSnL1MQijR7fZA (Supplemental Figure S10A) ------- COMMENT: dd3170869113df7a 38 SEah3c1fAaTjHtSnL1MQijR7fZA (Supplemental Figure S10A) ------- COMMENT: dd3170869113df7a 39 SEah3c1fAaTjHtSnL1MQijR7fZA (Supplemental Figure S10A) ------- COMMENT: dd3170869113df7a 40 tAHUrz73BFr3cwRJRvaEfaoI//k (Supplemental Figure S11B) ------- COMMENT: dd3170869113df7a 41 tAHUrz73BFr3cwRJRvaEfaoI//k (Supplemental Figure S11B) ------- COMMENT: dd3170869113df7a 42 xtsD2UJbMCt6Oy0Hk8hSMC4comU (comment: CHECK localizations at spindle poles during meiotic anaphase I) (Figure 6, Supplemental Figure S6) ------- COMMENT: dd3170869113df7a 44 xtsD2UJbMCt6Oy0Hk8hSMC4comU (comment: CHECK localizations at spindle poles during meiotic anaphase I) (Figure 6, Supplemental Figure S6) ------- COMMENT: dd3467c8fe93ae16 1 68gFz4CsjRUCJ07IlncqO281oBw Dis1 uses its TOG domains to induce microtubule catastrophe, in which polymerisation turns into depolymerisation ------- COMMENT: dd3467c8fe93ae16 2 tN/dyDxxphb/QcUkcoQZMj5eZUA Figure 2a ------- COMMENT: dd3467c8fe93ae16 6 PH7YHjDZjnbyyT3bzWdVFhZA0eo (comment: CHECK Evidence: in vitro biochemical assays using purified tubulin and recombinant Dis1 protein / New GO term requested: microtubule destabilization activity) ------- COMMENT: dd3467c8fe93ae16 9 tN/dyDxxphb/QcUkcoQZMj5eZUA Figure 2a ------- COMMENT: dd3467c8fe93ae16 10 tN/dyDxxphb/QcUkcoQZMj5eZUA Figure 2a ------- COMMENT: dd81766bd2cc050a 7 27wD8l6ipbmXSYcdGFjhpwlwSwE (comment: dependent on actin cytoskeleton (assayed using Latrunculin A)) ------- COMMENT: dd81766bd2cc050a 9 27wD8l6ipbmXSYcdGFjhpwlwSwE (comment: dependent on actin cytoskeleton (assayed using Latrunculin A)) ------- COMMENT: dd81766bd2cc050a 10 27wD8l6ipbmXSYcdGFjhpwlwSwE (comment: dependent on actin cytoskeleton (assayed using Latrunculin A)) ------- COMMENT: dd81766bd2cc050a 11 27wD8l6ipbmXSYcdGFjhpwlwSwE (comment: dependent on actin cytoskeleton (assayed using Latrunculin A)) ------- COMMENT: dda3e52a2fc3105f 3 7VGL1m59wrDN9HgQnNzJeCS8Wck (comment: severity estimated because wt (i.e. not overexpressing cdc25) not shown) ------- COMMENT: dda3e52a2fc3105f 4 7VGL1m59wrDN9HgQnNzJeCS8Wck (comment: severity estimated because wt (i.e. not overexpressing cdc25) not shown) ------- COMMENT: dda3e52a2fc3105f 5 7VGL1m59wrDN9HgQnNzJeCS8Wck (comment: severity estimated because wt (i.e. not overexpressing cdc25) not shown) ------- COMMENT: dda3e52a2fc3105f 6 7VGL1m59wrDN9HgQnNzJeCS8Wck (comment: severity estimated because wt (i.e. not overexpressing cdc25) not shown) ------- COMMENT: ddb5158a6caf4996 1 pzcfEg+xymV3LdVlQrKO0MVO0os MS analyses revealed a tenfold reduction of Tra1 from SAGA when Tti2 is depleted, as compared with control conditions (Fig. 2a). Similarly, we observed about a twofold reduction of Tra2 levels from NuA4 (Fig. 2b). Both approaches showed decreased interaction between newly synthesised Tra1 and affinity purified Spt7 in cells partially depleted of Tel2. These results demonstrate that TTT contributes to the de novo incorporation of Tra1 into the SAGA complex. ------- COMMENT: ddb5158a6caf4996 2 1nyMgHHtwZk+r0WLxlTzD8d+ews . Similarly, we observed about a twofold reduction of Tra2 levels from NuA4 (Fig. 2b). ------- COMMENT: ddb5158a6caf4996 6 dLT8cRYlRsj+MbcAwmOxrlTF14g (comment: RNA-seq) ------- COMMENT: ddb5158a6caf4996 8 dLT8cRYlRsj+MbcAwmOxrlTF14g (comment: RNA-seq) ------- COMMENT: ddb5158a6caf4996 10 dLT8cRYlRsj+MbcAwmOxrlTF14g (comment: RNA-seq) ------- COMMENT: ddb5158a6caf4996 11 QEq183tmFP4m53SyU7pu4+icMRI (Fig.1e, 1f) (comment: CHECK spt7/tra1 pho84 and mei2 promoters. and spt7/tras ssa2 promoter) ------- COMMENT: ddb5158a6caf4996 13 kRV4CTJT1utgHTKiEsYFUco3KZ0 Decreased levels of Tra1 and Tra2 in SAGA and NuA4 complexes, respectively Figure 2 ------- COMMENT: ddb5158a6caf4996 15 Mgxywe6gt3RpANdwFX312Zd89M4 See Figure 3a, 3b Describes the biogenesis of a multisubunit complex from nascent proteins. (comment: Could be linked to pombase ID of one or several components of the complex) ------- COMMENT: ddb5158a6caf4996 17 bHVnW3J0KH1rHOB8CIETgL/7lao See Figure 3c, 3d ------- COMMENT: ddb5158a6caf4996 42 nt75/YxyOseu+GZlw/HmfqvH+T0 See Figure 4 ------- COMMENT: ddb5158a6caf4996 45 Uj45aKiX1pCJIKFiDZhao2sb3lA (Fig. 5d) Phenotypic analyses of tra1-Sptra2 and tra1-Sctra1 strains showed that tra1-Sptra2 mutants are sensitive to HU and caffeine, similar to tra1Δ mutants ------- COMMENT: ddb5158a6caf4996 47 dLT8cRYlRsj+MbcAwmOxrlTF14g (comment: RNA-seq) ------- COMMENT: ddb5158a6caf4996 48 bXIxM8IkyKiHalQ1Xxqph+bCn0s Abolished Tra1 interaction with SAGA complex spt20Δ mutants, without any other visible changes in its overall migration profile (Fig. 6a). Spt20 is therefore essential for Tra1 incorporation into SAGA. ------- COMMENT: ddb5158a6caf4996 49 ZsrHsp3wfiJHsEKxV4NHhzdyXfA Abolished Tra1 interaction with SAGA complex ------- COMMENT: ddb5158a6caf4996 50 ZsrHsp3wfiJHsEKxV4NHhzdyXfA Abolished Tra1 interaction with SAGA complex ------- COMMENT: ddb5158a6caf4996 51 ZsrHsp3wfiJHsEKxV4NHhzdyXfA Abolished Tra1 interaction with SAGA complex ------- COMMENT: ddb5158a6caf4996 52 ZsrHsp3wfiJHsEKxV4NHhzdyXfA Abolished Tra1 interaction with SAGA complex ------- COMMENT: ddb5158a6caf4996 53 Fg4zAy6r4a7tK/LIPZ8mZC3r84A Decreased Tra1 interaction with SAGA complex ------- COMMENT: ddb5158a6caf4996 54 uDYD2l5WX7IW/3ZZJp1vG0Rgu5s (Fig. 5d) ------- COMMENT: ddb5158a6caf4996 59 dLT8cRYlRsj+MbcAwmOxrlTF14g (comment: RNA-seq) ------- COMMENT: ddb5158a6caf4996 60 dLT8cRYlRsj+MbcAwmOxrlTF14g (comment: RNA-seq) ------- COMMENT: ddb5158a6caf4996 61 XHCvLq9vkSTV3CzMr0xoOInmjkw We thus focused our investigations on Tti2, which we confirmed interacts with both Tra1 and Tra2 in S. pombe (Supplementary Data 1) ------- COMMENT: ddb5158a6caf4996 62 XHCvLq9vkSTV3CzMr0xoOInmjkw We thus focused our investigations on Tti2, which we confirmed interacts with both Tra1 and Tra2 in S. pombe (Supplementary Data 1) ------- COMMENT: ddb5158a6caf4996 63 R0SENrkiBgg2tFA81vVAuEx1/hk (Supplementary Data 1) ------- COMMENT: ddb5158a6caf4996 64 R0SENrkiBgg2tFA81vVAuEx1/hk (Supplementary Data 1) ------- COMMENT: ddb5158a6caf4996 65 R0SENrkiBgg2tFA81vVAuEx1/hk (Supplementary Data 1) ------- COMMENT: ddb5158a6caf4996 66 R0SENrkiBgg2tFA81vVAuEx1/hk (Supplementary Data 1) ------- COMMENT: ddb5158a6caf4996 67 R0SENrkiBgg2tFA81vVAuEx1/hk (Supplementary Data 1) ------- COMMENT: ddb5158a6caf4996 68 R0SENrkiBgg2tFA81vVAuEx1/hk (Supplementary Data 1) ------- COMMENT: ddb5158a6caf4996 69 8VPoWW0w123VyWzAbUWtuIPbsYQ Silver staining analysis showed that Hsp90 inactivation causes a specific decrease of Tra1 in Spt7 purification eluates (Supplementary Fig. 6). effect is modest in this hypomorphic mutant, this observation supports the conclusion that Hsp90, like TTT, contributes to the de novo assembly of Tra1 into SAGA. ------- COMMENT: ddb5158a6caf4996 70 GCgGEurPC5gkSpnURRuVxLt9S8A Tra2 contributes to the scaffolding and stabilisation of the entire NuA4 complex. ------- COMMENT: ddb5158a6caf4996 71 qOH0dlQUxoATqzycrTLuWFo5k08 Such distinct architectural roles provide a functional validation of the recent structural studies of yeast SAGA and NuA4, which showed that Tra1 occupies a peripheral position within SAGA ------- COMMENT: ddb5158a6caf4996 72 3TabY4d0U060pxdF0ztOxv3cy+A Impor- tantly, quantitative MS analyses show that both Tra1-SpTra2 and Tra1-ScTra1 hybrid mutant proteins efficiently copurify with Tti2 (Supplementary Fig. 9b). ------- COMMENT: ddb5158a6caf4996 73 1ceiUUUVoMbAJT4+5A6Lw1J8MhM whereas tra1-Sctra1 strains show no growth defects, as compared with wild-type cells (Fig. 5d). ------- COMMENT: ddb5158a6caf4996 74 1ceiUUUVoMbAJT4+5A6Lw1J8MhM whereas tra1-Sctra1 strains show no growth defects, as compared with wild-type cells (Fig. 5d). ------- COMMENT: ddb5158a6caf4996 75 dLT8cRYlRsj+MbcAwmOxrlTF14g (comment: RNA-seq) ------- COMMENT: ddb5158a6caf4996 76 LfzqQqC72zmCT8oxWd50NOSteHU Normal Tra1 interaction with SAGA complex ------- COMMENT: ddb5158a6caf4996 80 kRV4CTJT1utgHTKiEsYFUco3KZ0 Decreased levels of Tra1 and Tra2 in SAGA and NuA4 complexes, respectively Figure 2 ------- COMMENT: ddcbe348d70876b6 1 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: ddcbe348d70876b6 2 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: ddcbe348d70876b6 3 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: ddcbe348d70876b6 4 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: ddcbe348d70876b6 5 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: dde3c6ac603a05eb 31 T7L8A3vGKezVIUWh99YvVOXa6d0 (comment: CHECK qualifier=major) ------- COMMENT: ddf4869fba46e0a3 2 9ARh6JqlL7bH7FNLsWfdeV/twss (comment: CHECK microtubule sliding brake https://www.ebi.ac.uk/interpro/entry/InterPro/IPR007882/#PUB00070924) ------- COMMENT: de3fe3d88f14e4a8 107 rcpeYUEthA4KwPAdtbMmjEQ5ykw (comment: CHECK homozygous cross) ------- COMMENT: de3fe3d88f14e4a8 108 rcpeYUEthA4KwPAdtbMmjEQ5ykw (comment: CHECK homozygous cross) ------- COMMENT: de3fe3d88f14e4a8 109 rcpeYUEthA4KwPAdtbMmjEQ5ykw (comment: CHECK homozygous cross) ------- COMMENT: de3fe3d88f14e4a8 179 goEO9S3UHQnaYolcF2Vye6cN6UI (comment: dependent on F-actin (assayed using Latrunculin A)) ------- COMMENT: de3fe5786898e2f8 1 bMSLNWuDs5xF0B20HEbeLIIyksc Figure 4E ((comment: moved from wee) (skewed towards small, low severity)) ------- COMMENT: de3fe5786898e2f8 2 rudolhzPalYa91VrHIhDrrYEQ+w increased binding by about 2 fold from Figure 4C, 4D ------- COMMENT: de3fe5786898e2f8 3 mPLJ0kLlAEXFIS7dynECbFNF+Mc Figure 3A, 3B (comment: Live-cell time-lapse imaging) ------- COMMENT: de3fe5786898e2f8 4 ACd0Khan3fss/GUDKl1JW5lxmeI Figure 1D-G (comment: live cell imaging) ------- COMMENT: de3fe5786898e2f8 5 29wbBNLWbfI55wN0QPZGByJfLIQ Figure 2A, 2B ((comment: vw changed to cell division site during M-phase from septum) (live cell imaging)) ------- COMMENT: de3fe5786898e2f8 6 mPLJ0kLlAEXFIS7dynECbFNF+Mc Figure 3A, 3B (comment: Live-cell time-lapse imaging) ------- COMMENT: de3fe5786898e2f8 7 mPLJ0kLlAEXFIS7dynECbFNF+Mc Figure 3A, 3B (comment: Live-cell time-lapse imaging) ------- COMMENT: de3fe5786898e2f8 8 7sLB7dedHX5igluytx8lO0+xlGg Figure 4A, 4B (comment: live cell imaging) ------- COMMENT: de3fe5786898e2f8 9 K4MUIihoS3JKduEOVAn2zzJKtus Figure 2E, 2F (comment: live cell imaging) ------- COMMENT: de3fe5786898e2f8 10 axfqMh/dA9C0wVB8U7as7jvTPXw Figure 4G (comment: live cell DIC) ------- COMMENT: de3fe5786898e2f8 11 wNBCPzy7nCcThN3Enlk3crNzLz0 Figure S4F, S4H (comment: live cell DIC) ------- COMMENT: de3fe5786898e2f8 12 QkF/+dmQdG7xOypSi8oqrdXwHS0 Figure S4F, S4G (comment: (vw move to FYPO:0006822 and requested parentage fix in FYPO) (live cell DIC)) ------- COMMENT: de3fe5786898e2f8 13 QkF/+dmQdG7xOypSi8oqrdXwHS0 Figure S4F, S4G (comment: (vw move to FYPO:0006822 and requested parentage fix in FYPO) (live cell DIC)) ------- COMMENT: de3fe5786898e2f8 14 mDjXqo0i+AU9KRv8VR9BrUDqj4g Figure S4C, S4D ------- COMMENT: de3fe5786898e2f8 15 2e9R2PqwAY5PBUEBnMhxKtHnlFs Figure 3D (comment: live cell DIC) ------- COMMENT: de3fe5786898e2f8 16 O9cVgzjFJMaO506ceUlnYGWZxTA Figure 3C, ,3D ------- COMMENT: de3fe5786898e2f8 17 hyHqoUxQgii9GcVUmwtSUlJDj+Q Figure 1H, 1I (comment: live cell imaging) ------- COMMENT: de3fe5786898e2f8 18 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: de3fe5786898e2f8 19 VUTPcxd5eFHpD12TE/Iuh8Oxj9U Figure S2A, S2B (comment: (vw changed from FYPO:0001677 to FYPO:0002874 to match rlc1) (live cell imaging)) ------- COMMENT: de3fe5786898e2f8 20 VUTPcxd5eFHpD12TE/Iuh8Oxj9U Figure S2A, S2B (comment: (vw changed from FYPO:0001677 to FYPO:0002874 to match rlc1) (live cell imaging)) ------- COMMENT: de3fe5786898e2f8 21 VUTPcxd5eFHpD12TE/Iuh8Oxj9U Figure S2A, S2B (comment: (vw changed from FYPO:0001677 to FYPO:0002874 to match rlc1) (live cell imaging)) ------- COMMENT: de3fe5786898e2f8 22 ta8BmvtiW2gvhkEp6/TdaHOWBL8 Figure S2A, S2B (comment: live cell imaging) ------- COMMENT: de3fe5786898e2f8 23 grc7Df6YETd9VIXKzM+GKPmappQ Figure S2C (comment: live cell imaging) ------- COMMENT: de3fe5786898e2f8 24 1KeCGf9qTqYZ0T+VN3+D0m1tgi0 Figure S3A (comment: live cell imaging) ------- COMMENT: de3fe5786898e2f8 25 8j3dKxbmgVI/iTx9Vip7Uy02/To Figure S3C, S3D (comment: live cell imaging) ------- COMMENT: de3fe5786898e2f8 28 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: de3fe5786898e2f8 29 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: de3fe5786898e2f8 30 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: de3fe5786898e2f8 32 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: de3fe5786898e2f8 34 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: de3fe5786898e2f8 35 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: de3fe5786898e2f8 37 +eox+EeRxTJq36zRWUrxf2Xu3j0 Figure 4I (comment: Live-cell time-lapse imaging) ------- COMMENT: de3fe5786898e2f8 38 axfqMh/dA9C0wVB8U7as7jvTPXw Figure 4G (comment: live cell DIC) ------- COMMENT: de3fe5786898e2f8 39 axfqMh/dA9C0wVB8U7as7jvTPXw Figure 4G (comment: live cell DIC) ------- COMMENT: de3fe5786898e2f8 40 axfqMh/dA9C0wVB8U7as7jvTPXw Figure 4G (comment: live cell DIC) ------- COMMENT: de3fe5786898e2f8 41 jT2cYqUtcP6G/z3rmUwHkETaMyA Figure 4H (comment: (vw moved down to FYPO:0003481) (live cell DIC)) ------- COMMENT: de3fe5786898e2f8 42 irg60QmNXxlNcAh6xst4ob6r3r0 Figure S4G (comment: (vw moved down to FYPO:0003481) (live cell DIC)) ------- COMMENT: de3fe5786898e2f8 43 EPhYqNiozKzwhlK6AAmrtuoQDEk Figure S4F, S4H (comment: (vw move to FYPO:0006822 and requested parentage fix in FYPO) (live cell DIC)) ------- COMMENT: de3fe5786898e2f8 44 DF+zIP3JThih2tzXHBdFP1s0WpE Figure S4A (comment: live cell imaging) ------- COMMENT: de3fe5786898e2f8 45 DF+zIP3JThih2tzXHBdFP1s0WpE Figure S4A (comment: live cell imaging) ------- COMMENT: de3fe5786898e2f8 46 1KeCGf9qTqYZ0T+VN3+D0m1tgi0 Figure S3A (comment: live cell imaging) ------- COMMENT: de3fe5786898e2f8 47 8j3dKxbmgVI/iTx9Vip7Uy02/To Figure S3C, S3D (comment: live cell imaging) ------- COMMENT: de3fe5786898e2f8 48 hyHqoUxQgii9GcVUmwtSUlJDj+Q Figure 1H, 1I (comment: live cell imaging) ------- COMMENT: de3fe5786898e2f8 49 W+ZR5n636jfyNSYDhFOu+zi36e4 Figure 3C, 3D (comment: live cell DIC) ------- COMMENT: de3fe5786898e2f8 50 As21B5r3vD7064Bd0vdL3dvx4CM FIg 1D ------- COMMENT: de3fe5786898e2f8 51 As21B5r3vD7064Bd0vdL3dvx4CM FIg 1D ------- COMMENT: de3fe5786898e2f8 52 As21B5r3vD7064Bd0vdL3dvx4CM FIg 1D ------- COMMENT: de3fe5786898e2f8 53 8HDDb9X9tViW9o/gXtxjqYiVHAc Fig 2E, 2F ------- COMMENT: de3fe5786898e2f8 56 A1vhy0FYK+SoURt6KK+A31i4v2s Figure S4A ------- COMMENT: de7fb03a2e70fba6 1 09Q/EoJb/tfgb6C5sPdO7QeHIdU (comment: Alp7 physically interacts with Mto1) ------- COMMENT: de87f5175641f7e9 2 RoLmRGOosEG5lI7cSNp+i6Yi4Yc (comment: CHECK Rad21) ------- COMMENT: de87f5175641f7e9 9 a1dM/ys/9G8EDQ9Dfhxcm3CjECg Figure 3A ------- COMMENT: de87f5175641f7e9 13 f4B5HFBwyFT+1/oXr0vQB8WOmic Figure 3A (comment: but not S phase) ------- COMMENT: de87f5175641f7e9 15 VTSEkCX3QnkLWEHeLemrmNUZOTA Supplementary Figure S2 ------- COMMENT: de87f5175641f7e9 16 z9w0SEt8I3Cs2kUPE3oPFFTPwJo Figure 3A, 3B ------- COMMENT: de87f5175641f7e9 17 vmnwBusghfhTiVXn8a2uo5sEJjg Figure 3C, 3E ------- COMMENT: de87f5175641f7e9 19 Gqeq4bgokS4vto+Wii//nX7w79k Figure 3B ------- COMMENT: de87f5175641f7e9 20 Gqeq4bgokS4vto+Wii//nX7w79k Figure 3B ------- COMMENT: de87f5175641f7e9 21 Hu0TKFbj+juYQdq1e4Y7aKUyjsg Figure 3C, 3E ------- COMMENT: de87f5175641f7e9 22 Hu0TKFbj+juYQdq1e4Y7aKUyjsg Figure 3C, 3E ------- COMMENT: de87f5175641f7e9 23 YyWqIXPf/R5o6nrgL+4ODvgQsjQ Figure 3C, 3E (comment: CHECK during G1) ------- COMMENT: de87f5175641f7e9 25 ZEJOBQzZSVfZOSx1dzYWKXc/26s (comment: maintenence) ------- COMMENT: de8b67d975d050e6 5 pr9uCL6u9WrGM4PyHRRDXt/8BO0 fig s1, (comment: can't tell after germination or before) ------- COMMENT: dea291f22cf98fe2 1 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: dea291f22cf98fe2 2 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: dea291f22cf98fe2 3 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: dea291f22cf98fe2 4 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: dea291f22cf98fe2 5 U/PvCZ3/sYCTc+VkXd+IsT2uQcI fig1b ------- COMMENT: dea291f22cf98fe2 6 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: dea291f22cf98fe2 7 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: dea291f22cf98fe2 8 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: dea291f22cf98fe2 9 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: dea291f22cf98fe2 10 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: dea291f22cf98fe2 11 rHLtAeGByyusDPeGHLYxWSnIsQk figure1e ------- COMMENT: dea291f22cf98fe2 12 rHLtAeGByyusDPeGHLYxWSnIsQk figure1e ------- COMMENT: dea291f22cf98fe2 13 rHLtAeGByyusDPeGHLYxWSnIsQk figure1e ------- COMMENT: dea291f22cf98fe2 14 rHLtAeGByyusDPeGHLYxWSnIsQk figure1e ------- COMMENT: dea291f22cf98fe2 15 rHLtAeGByyusDPeGHLYxWSnIsQk figure1e ------- COMMENT: dea291f22cf98fe2 17 ZKeBmFlyBQP586HTw2KU8J7r9D0 (comment: D.N.S?) ------- COMMENT: dea291f22cf98fe2 18 ZKeBmFlyBQP586HTw2KU8J7r9D0 (comment: D.N.S?) ------- COMMENT: dea291f22cf98fe2 19 ZKeBmFlyBQP586HTw2KU8J7r9D0 (comment: D.N.S?) ------- COMMENT: dea291f22cf98fe2 20 5GvajknUqhN6j1MJC8ho7ecMmTg Pst1p colocalizes with the otr/imr region in a cell cycle-specific manner. ------- COMMENT: dea291f22cf98fe2 21 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: dea291f22cf98fe2 22 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: dea291f22cf98fe2 24 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: dea291f22cf98fe2 25 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: dea291f22cf98fe2 26 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: dea291f22cf98fe2 27 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: dea291f22cf98fe2 28 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: dea291f22cf98fe2 29 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: deaffb81d6a5dd3d 1 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: deaffb81d6a5dd3d 2 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: deaffb81d6a5dd3d 3 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: deaffb81d6a5dd3d 5 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: dee2cb4000771ecf 2 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: dee2cb4000771ecf 3 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: dee2cb4000771ecf 4 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: dee2cb4000771ecf 5 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: dee2cb4000771ecf 6 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: dee2cb4000771ecf 7 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: dee2cb4000771ecf 8 6szRBP/6KDsT9HbtpHjWgtrzVlo Fig 1D (comment: decreased rate of cell separation) ------- COMMENT: dee2cb4000771ecf 9 1s05bTafPMW8D1W8Sn9w2UUXEVc Fig 1D ------- COMMENT: dee2cb4000771ecf 10 PCfvBOxnF37/BpYFJ2UuNLcJ1DY Fig 1E ------- COMMENT: dee2cb4000771ecf 11 PCfvBOxnF37/BpYFJ2UuNLcJ1DY Fig 1E ------- COMMENT: dee2cb4000771ecf 12 iRc67pN5dJpwz7PrdHUbbP1yjfo fig 1F ------- COMMENT: dee2cb4000771ecf 13 DvTitOYXOd9kCnMv+p50XBXgODc Fig 1F ------- COMMENT: dee2cb4000771ecf 14 DvTitOYXOd9kCnMv+p50XBXgODc Fig 1F ------- COMMENT: dee2cb4000771ecf 15 iRc67pN5dJpwz7PrdHUbbP1yjfo fig 1F ------- COMMENT: dee2cb4000771ecf 16 6YcGa1grLs/+5nYfCv75tYVHHJE Figure 3C ------- COMMENT: dee2cb4000771ecf 17 6YcGa1grLs/+5nYfCv75tYVHHJE Figure 3C ------- COMMENT: dee2cb4000771ecf 18 CQGtsMsb1Fz3rI4URnEB3BTVeqI Data not shown ------- COMMENT: dee2cb4000771ecf 19 CQGtsMsb1Fz3rI4URnEB3BTVeqI Data not shown ------- COMMENT: dee2cb4000771ecf 20 tOmOPs9JendY5r39VoSXnsV78ik Fig. 3F ------- COMMENT: dee2cb4000771ecf 21 tOmOPs9JendY5r39VoSXnsV78ik Fig. 3F ------- COMMENT: dee2cb4000771ecf 22 tOmOPs9JendY5r39VoSXnsV78ik Fig. 3F ------- COMMENT: dee2cb4000771ecf 23 CQGtsMsb1Fz3rI4URnEB3BTVeqI Data not shown ------- COMMENT: dee2cb4000771ecf 24 CQGtsMsb1Fz3rI4URnEB3BTVeqI Data not shown ------- COMMENT: dee2cb4000771ecf 25 +t2qCCGlK6xrXs1Rvm4vTpyZddI Fig 4A ------- COMMENT: dee2cb4000771ecf 26 +t2qCCGlK6xrXs1Rvm4vTpyZddI Fig 4A ------- COMMENT: dee2cb4000771ecf 27 +t2qCCGlK6xrXs1Rvm4vTpyZddI Fig 4A ------- COMMENT: dee2cb4000771ecf 28 +t2qCCGlK6xrXs1Rvm4vTpyZddI Fig 4A ------- COMMENT: dee2cb4000771ecf 29 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: dee2cb4000771ecf 30 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: dee2cb4000771ecf 31 8h7AKxnXg3VYc3EmbEFSPlRaQ24 Fig 4C, 4D, 4E ------- COMMENT: dee2cb4000771ecf 32 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: dee2cb4000771ecf 33 /1i8cPDCuLvucB7JYHREqQ2SRMs Fig 5A ------- COMMENT: dee2cb4000771ecf 34 3XDMzCzKn7uinkqE2kGUWXwZUD8 Fig 5B ------- COMMENT: dee2cb4000771ecf 36 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: dee2cb4000771ecf 37 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: dee2cb4000771ecf 38 NPv24odko/WPxsMYQ5cPiX5H/Xk Fig 6B. ------- COMMENT: dee2cb4000771ecf 39 NPv24odko/WPxsMYQ5cPiX5H/Xk Fig 6B. ------- COMMENT: dee2cb4000771ecf 40 NPv24odko/WPxsMYQ5cPiX5H/Xk Fig 6B. ------- COMMENT: dee2cb4000771ecf 41 NPv24odko/WPxsMYQ5cPiX5H/Xk Fig 6B. ------- COMMENT: dee2cb4000771ecf 42 NPv24odko/WPxsMYQ5cPiX5H/Xk Fig 6B. ------- COMMENT: dee2cb4000771ecf 43 NPv24odko/WPxsMYQ5cPiX5H/Xk Fig 6B. ------- COMMENT: dee2cb4000771ecf 44 QDyddrwuAdd2B2gpVt1pG+uRx9A Fig 6C. ------- COMMENT: dee2cb4000771ecf 45 jsz7/5UyzGUwWGLd2CjzU5vzU1s Fig 6C, 6D ------- COMMENT: dee2cb4000771ecf 47 EuTQLfr9dKOMeyAyfYXsM2WmdoE Fig 6D. ------- COMMENT: dee2cb4000771ecf 48 XwjFKIBpFnAfCL+3uv8ZHt+X4Cc (Fig. 6D) ------- COMMENT: df0514b2d11d29a7 1 OANZSU9csJMhMwLPI+uzFfjItDY The results revealed that histone H3 was the only histone methylated (Fig. 3B). ------- COMMENT: df0514b2d11d29a7 2 cscmCXcnzkm1+EgMYo5tERKRH1g As shown in ​Fig. Fig.3D,3D, SpSet2 was able to methylate an H3 peptide of residues 27 to 45, but not that of an H3 N-terminal peptide (residues 1 to 20). These data demonstrate that SpSet2 is a robust nucleosome-selective HMT specific for K36 methylation. ------- COMMENT: df0514b2d11d29a7 3 OANZSU9csJMhMwLPI+uzFfjItDY The results revealed that histone H3 was the only histone methylated (Fig. 3B). ------- COMMENT: df0514b2d11d29a7 4 OANZSU9csJMhMwLPI+uzFfjItDY The results revealed that histone H3 was the only histone methylated (Fig. 3B). ------- COMMENT: df0514b2d11d29a7 5 XIkXybad4Ns9W2lM468/Y46ZR9M As shown in Fig. 4A, dele- tion of set2 resulted in a complete abolishment of K36 meth- ylation (mono-, di-, and trimethylation), but not K4 methyl- ation or H3 K9 acetylation, in bulk histones ------- COMMENT: df0514b2d11d29a7 6 GpeLQJ1RNkg8K90uGYCjHV8GO7w et2 cells grew nor- mally on rich YEA medium, they showed a strong growth defect in synthetic medium (EMM), which is nutrient depleted compared to YEA (Fig. 4B). ------- COMMENT: df0514b2d11d29a7 7 GpeLQJ1RNkg8K90uGYCjHV8GO7w et2 cells grew nor- mally on rich YEA medium, they showed a strong growth defect in synthetic medium (EMM), which is nutrient depleted compared to YEA (Fig. 4B). ------- COMMENT: df0514b2d11d29a7 8 cscmCXcnzkm1+EgMYo5tERKRH1g As shown in Fig. ​Fig.3D,3D, SpSet2 was able to methylate an H3 peptide of residues 27 to 45, but not that of an H3 N-terminal peptide (residues 1 to 20). These data demonstrate that SpSet2 is a robust nucleosome-selective HMT specific for K36 methylation. ------- COMMENT: df0514b2d11d29a7 9 cscmCXcnzkm1+EgMYo5tERKRH1g As shown in Fig. ​Fig.3D,3D, SpSet2 was able to methylate an H3 peptide of residues 27 to 45, but not that of an H3 N-terminal peptide (residues 1 to 20). These data demonstrate that SpSet2 is a robust nucleosome-selective HMT specific for K36 methylation. ------- COMMENT: df0514b2d11d29a7 10 cscmCXcnzkm1+EgMYo5tERKRH1g As shown in Fig. ​Fig.3D,3D, SpSet2 was able to methylate an H3 peptide of residues 27 to 45, but not that of an H3 N-terminal peptide (residues 1 to 20). These data demonstrate that SpSet2 is a robust nucleosome-selective HMT specific for K36 methylation. ------- COMMENT: df0514b2d11d29a7 11 NOoRSUBKd8rm1tKY1qB2iyvRbjY . No unmodifiedPol II could be detected in these immunoprecipitates, althoughunmodified Pol II could be readily detected in the input extracts. These data demonstrate that SpSet2 is associated withthe elongating form of Pol II in S. pombe. ------- COMMENT: df17d2db3d735794 1 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: df17d2db3d735794 3 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: df17d2db3d735794 4 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: df17d2db3d735794 5 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: df17d2db3d735794 6 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: df17d2db3d735794 7 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: df17d2db3d735794 8 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: df17d2db3d735794 9 kA3kGbF8iJsR6qx2YGquKOVX+gI Extended Data Figure 9e ------- COMMENT: df1fa844a75ee0cf 1 Gn6Z5NTXceRPjy6XUep+MZt1zkw (comment: spectra looks the same as Adx) ------- COMMENT: df782d43e0f73c73 18 9SnPQiwfdQT10QSsE9tbXHY6u0E (comment: longer transcript) ------- COMMENT: df782d43e0f73c73 23 9SnPQiwfdQT10QSsE9tbXHY6u0E (comment: longer transcript) ------- COMMENT: df782d43e0f73c73 33 9SnPQiwfdQT10QSsE9tbXHY6u0E (comment: longer transcript) ------- COMMENT: dfae0c881b3c5469 55 max4HEZNUxQSXwmzYVBOGtNvtHw (comment: CONDITION 25 degrees) ------- COMMENT: dfae0c881b3c5469 56 ewfspod7Bin5Lx5/B/NV8EarpfI (comment: CONDITION 30 degrees) ------- COMMENT: dfb57891720b2902 8 SBGdDp+jzrp1KVnWxZEclIz/rmY (comment: vw: author intent) ------- COMMENT: dfb57891720b2902 9 SBGdDp+jzrp1KVnWxZEclIz/rmY (comment: vw: author intent) ------- COMMENT: dfb57891720b2902 10 SBGdDp+jzrp1KVnWxZEclIz/rmY (comment: vw: author intent) ------- COMMENT: dfb57891720b2902 11 B93A+maKtJHxf/snzZYH50I4AJM (comment: vw: binds DNA on its own) ------- COMMENT: dfb57891720b2902 12 LM4TgAnV3Fz8GWlZ7xfkVtPm7xM (comment: vw: binds DNA in complex (2,3,11)) ------- COMMENT: dfb57891720b2902 13 LM4TgAnV3Fz8GWlZ7xfkVtPm7xM (comment: vw: binds DNA in complex (2,3,11)) ------- COMMENT: dfb57891720b2902 14 LM4TgAnV3Fz8GWlZ7xfkVtPm7xM (comment: vw: binds DNA in complex (2,3,11)) ------- COMMENT: dfb57891720b2902 15 SBGdDp+jzrp1KVnWxZEclIz/rmY (comment: vw: author intent) ------- COMMENT: dfbcec2a0269ec27 1 8Equ2ecptlTW0a3m478ie4ss8R8 As expected, Rng2 localized to the nodes and ring upon entry into mitosis (Figure 4B), w ------- COMMENT: dfbcec2a0269ec27 2 n48YNn6eOuUPZPgJlB++M/GTtZU whereas the CHD (aa 1–190) localized to all F-actin structures including actin cables, actin patches, and the actin ring (Figure 4C and Video S3).44,45 ------- COMMENT: dfbcec2a0269ec27 4 1ZB6gUY+TxcWWPOEWhgYzWBl35w Strikingly, the Rng2 fragment (aa 1–300), previously called Rng2Ns,44 began to localize to the division site at the onset of anaphase and constricted during cytokinesis (Figure 4D and Video S3) ------- COMMENT: dfbcec2a0269ec27 5 U07dbucXfYCNYAeIV/QLK4uV14I Indeed, the fragment (aa 185–300) containing the CykF domain localized weakly, but consistently, to the division site during cytokinesis (Figures 4E and S4A and Video S3), suggesting that the binding affinity between CykF and the cytokinesis factor(s) is low. ------- COMMENT: dfbcec2a0269ec27 6 nRvz+/MEBgSNx6zAkBvaUwFyM/4 We found that the rng2-CHDΔ cells displayed similar cell and septum morphologies compared with the WT (rng2 +) cells at both temperatures (Figures 5B–5D and S4C). ------- COMMENT: dfbcec2a0269ec27 7 +5Aa6h+SLNa7plah74aPAEU0j+g The rng2-CykFΔ cells did not show any apparent defect in cell morphology, but ~10% of the cells formed a partial or complete septum doublet at both temperatures, suggesting a moderate defect in cytokinesis (Figures 5B–5D and S4C). ------- COMMENT: dfbcec2a0269ec27 8 lDMoF8Lmihj9BiM7cQRUADj/cVU However, when both domains were deleted together (rng2-CHDΔ-CykFΔ), most cells showed strong defects in cytokinesis, especially at 36°C, where >90% of the cells formed cell chains with various abnormal septa, including some “catastrophic” structures (Figures 5B–5D). ------- COMMENT: dfbcec2a0269ec27 9 lDMoF8Lmihj9BiM7cQRUADj/cVU However, when both domains were deleted together (rng2-CHDΔ-CykFΔ), most cells showed strong defects in cytokinesis, especially at 36°C, where >90% of the cells formed cell chains with various abnormal septa, including some “catastrophic” structures (Figures 5B–5D). ------- COMMENT: dfbcec2a0269ec27 10 y4qgrGanv2KUMtW6bAl+t4bxzlw GFP-Rng2-CHDΔ behaved like the WT in all aspects (Figures 5E–5H), except for the loss of accumulation during the slow phase (Figure 5F, from −15 to 0 min). ------- COMMENT: dfbcec2a0269ec27 11 1SHZ3pNfQI9NmbOIzD5kuwqhmw4 The removal of GFP-Rng2-CykFΔ was also slowed down (Figures 5E and 5F), and its rate of constriction was reduced by ~30% compared with GFP-Rng2 (Figure 5H, p < 0.01) ------- COMMENT: dfbcec2a0269ec27 12 4VN4lq1a4yB/pBQB518o2JM5n5Y s. Surprisingly, the rng2- CHDΔ cells formed WT-like actin rings, as revealed by phalloidin staining (Figure 6A), despite the ability of CHD to bind F-actin.3 ------- COMMENT: dfbcec2a0269ec27 13 Pev3FVHsHPLUHTAJhHhm5wJegqw The rng2-CykFΔ cells also assembled actin rings (Figures 6A and 6B), but the ring emergence and removal were significantly delayed (Figures 6B–6D) ------- COMMENT: dfd513564cb0ad94 1 rDAvogSwFon4g+MxC5cuG3yVMbU Our results revealed that Clr3 is indeed enriched through- out the 20 kb heterochromatic domain surrounded by the IR-R and IR-L boundary elements but is absent at the surrounding euchromatic regions (Figure 2A). ------- COMMENT: dfd513564cb0ad94 2 fBRa3dhwfk0y7mKRk7xVX7YqBQI Remarkably, loss of Swi6 and Chp2 but not Chp1 completely abolished the localization of Clr3 at Kint2::ura4+ (Figure 1) ------- COMMENT: dfd513564cb0ad94 3 fBRa3dhwfk0y7mKRk7xVX7YqBQI Remarkably, loss of Swi6 and Chp2 but not Chp1 completely abolished the localization of Clr3 at Kint2::ura4+ (Figure 1) ------- COMMENT: dfd513564cb0ad94 4 fBRa3dhwfk0y7mKRk7xVX7YqBQI Remarkably, loss of Swi6 and Chp2 but not Chp1 completely abolished the localization of Clr3 at Kint2::ura4+ (Figure 1) ------- COMMENT: dfd513564cb0ad94 5 7Gq74Yen8fFTYpHmzqO9Pchctqw In the absence of Swi6, Clr3 localization was confined to a small region near the mat3 locus (Figure 2A), which is distinct from the cenH element responsi- ble for RNAi-mediated targeting of heterochromatin to this region. ------- COMMENT: dfd513564cb0ad94 6 qDso6w0kDi3bCsDjGO9j2FqI0d8 mutant cells are de- fective in histone deacetylation and silencing at the mat2/3 locus (see Figure S1 ------- COMMENT: dfd513564cb0ad94 7 OMRKJtrAPThK7AcC2p9zyh1d1FI ChIP analysis revealed that the Clr3 mutant protein was mainly restricted to the nucleation site adjacent to the mat3 locus and the spreading of Clr3 across the mat2/3 region was se- verely affected (Figure 2A). ------- COMMENT: dfd513564cb0ad94 8 EWFtz//kR0LorsU9eHxKjdu5muo Surprisingly, except for a small but reproducible enrichment of Clr3 at the nu- cleation site, Clr3 was virtually absent from the entire mat2/3 region in a sir2D strain (Figure 2A). ------- COMMENT: dfd513564cb0ad94 9 /Z4COM7oYdXXMBnibv+Kb9bhopU These data, together with results showing defects in Swi6 localiza- tion at the mat locus in clr3-735 cells (see below; Figure S2), ------- COMMENT: dfd513564cb0ad94 10 Sk1/PFA43dqWbWvtmFOKy6qalGo (comment: [NUCLEATION/SPREADING]) Subsequently, Clr3 spreads across the entire mat2/3 interval that is dependent upon its own HDAC activity, Swi6 and Sir2 proteins, and possibly other fac- tors, such as Chp2, involved in heterochromatin as- sembly. These results suggest that Clr3 operates in a pathway parallel to RNAi to nucleate heterochromatin at the mat locus. ------- COMMENT: dfd513564cb0ad94 11 VUb+MqQQaunFRr+P05yHbWQKRw4 (comment: [SPREADING] ) Subsequently, Clr3 spreads across the entire mat2/3 interval that is dependent upon its own HDAC activity, Swi6 and Sir2 proteins, and possibly other fac- tors, such as Chp2, involved in heterochromatin as- sembly. ------- COMMENT: dfd513564cb0ad94 12 VUb+MqQQaunFRr+P05yHbWQKRw4 (comment: [SPREADING]) Subsequently, Clr3 spreads across the entire mat2/3 interval that is dependent upon its own HDAC activity, Swi6 and Sir2 proteins, and possibly other fac- tors, such as Chp2, involved in heterochromatin as- sembly. ------- COMMENT: dfd513564cb0ad94 13 ugqFhUPbTbnWOEAlGkkDc2BKDJk (comment: [ vw specifically to REIII/ CAS, nucleation site]) In double mutant cells lacking Pcr1 and Swi6, the localization of Clr3 was almost com- pletely abolished from REIII (Figure 3). ------- COMMENT: dfd513564cb0ad94 14 g2+UDC9GKpsn0bTH5rNkovbM25M Whereas H3K9me levels at Kint2:: ura4+ were not affected in clr3D, dcr1D, or atf1D single mutants compared to wild-type, H3K9me was com- pletely abolished in a clr3D dcr1D double mutant strain (Figure 4A). ------- COMMENT: dfd513564cb0ad94 15 VUb+MqQQaunFRr+P05yHbWQKRw4 (comment: [SPREADING] ) Subsequently, Clr3 spreads across the entire mat2/3 interval that is dependent upon its own HDAC activity, Swi6 and Sir2 proteins, and possibly other fac- tors, such as Chp2, involved in heterochromatin as- sembly. ------- COMMENT: dfd513564cb0ad94 16 CjXxGqlA7R5kcFJVBCSb9jY4yiM (comment: [NUCLEATION]) ------- COMMENT: dfd513564cb0ad94 17 QlQFyCr5cT0pgwiehT7oDSWYOHs (comment: [NUCLEATION] Same pathway as clr3) ------- COMMENT: dfd513564cb0ad94 18 ivxVwX46c99OBCqse6bws7hBMtY Remarkably, whereas clr3D or dcr1D single mutant strains still maintained H3K9 methylation, H3K9me at centromeric repeats was almost completely abolished in clr3Ddcr1D double mutant cells (Figure 4C). ------- COMMENT: dfd513564cb0ad94 19 fBRa3dhwfk0y7mKRk7xVX7YqBQI Remarkably, loss of Swi6 and Chp2 but not Chp1 completely abolished the localization of Clr3 at Kint2::ura4+ (Figure 1) ------- COMMENT: dfd513564cb0ad94 20 DxCJLLBuQL9ekAKWNpIg+aJf1LY ..... However, in clr3D cells, H3K9me3 was significantly reduced, while there was a substantial in- crease in H3K9me1. Furthermore, H3K9me2 levels were slightly elevated (Figure 5A). ------- COMMENT: dfd513564cb0ad94 21 WGCts9VQ4iMM4bxO1FZD+MUnrhg .....Interestingly, identical modifi- cation patterns were also observed in a swi6 mutant, consistent with Swi6 involvement in Clr3 spreading (Figure 5A). ------- COMMENT: dfd513564cb0ad94 22 zDhcsDkYznV0khMTOfRXXxUI0RE We next explored whether loss of Clr3 affects Swi6 binding at the mat locus. Deletion of clr3 resulted in severely reduced Swi6 levels at Kint2::ura4+ even though Swi6 expression was not affected (Figure 5C; Figure S3). ------- COMMENT: dfd513564cb0ad94 23 eLXaLH1HeW3yDarxJ8TrPCE9+Ks deletion of clr3 resulted in an increase in acetylation at H3K14 (H3K14ac) (Figure 6A), a mark of active chromatin that is absent at heterochromatic loci. ------- COMMENT: dfd513564cb0ad94 24 lmZ+am4A1+X1vQWyboP+oZoMlEY Furthermore, the levels of H3S10 phosphorylation (H3S10ph), another modification mark associated with active chromatin as well as mitotic chromosomes (Nowak and Corces, 2004), were also increased at the mat locus (Figure 6A). ------- COMMENT: dfd513564cb0ad94 25 60T2dnukzNMa3kmGnVZKuNy1HDQ Similar changes were observed in the swi6 mutant; however, the effect on H3S10ph was weaker than in the clr3 mutant (Figure 6A). ------- COMMENT: dfd513564cb0ad94 26 kFGZ4EhBDexlMwboHebGCw5ARH8 Our analyses revealed that a temper- ature-sensitive mutation in the survivin homolog Cut17/ Bir1 (cut17-275), which is known to bind centromeric repeats and is required for proper localization of fission yeast aurora kinase Ark1 (Morishita et al., 2001), almost completely abolished H3S10ph at the mat locus in clr3D cells (Figure 6C). ------- COMMENT: dfd513564cb0ad94 27 Vu+RX+9Ls8kkhE+YggQXGMi/B/k We show that Cir3, a fission yeast homolog of mammalian class |I HDACs, acts in a distinct pathway parallel to RNAi-directed heterochromatin nucleation to recruit Cl4 and mediate H3K9 methylation at the silent mating-type region and centromeres. ------- COMMENT: dfd513564cb0ad94 28 YE1P76dVmcnfT0UkGxf5mcRIKog At the mat locus, Clr3 is recruited at a specific site through a mechanism involving ATF/CREB family proteins ------- COMMENT: dfd6ea81df55ce19 5 lOu54XeY9tM7rNIuefMzwJE2PYc (comment: CHECK activated_by CHEBI:15422 | inhibited_by CHEBI:16284) ------- COMMENT: dfd6ea81df55ce19 6 lOu54XeY9tM7rNIuefMzwJE2PYc (comment: CHECK activated_by CHEBI:15422 | inhibited_by CHEBI:16284) ------- COMMENT: dff943277843288b 2 VZmWUOhWWbj9wM88G2BzsMrj8Js (comment: Evidence was from RNA-seq not from microarray) ------- COMMENT: dff943277843288b 3 pKJxf7lS47wLruzfB+CU1Dy9Clw (comment: Evidence code was RNA-seq) ------- COMMENT: dff943277843288b 4 9zu1E9lvvkTKmSJKnrYZRBdo+0Y (comment: Evidence from RNA-seq data) ------- COMMENT: dff943277843288b 5 9zu1E9lvvkTKmSJKnrYZRBdo+0Y (comment: Evidence from RNA-seq data) ------- COMMENT: dff943277843288b 6 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 7 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 8 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 9 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 10 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 11 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 12 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 13 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 14 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 15 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 16 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 17 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 18 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 19 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 20 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 21 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 22 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 23 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 24 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 25 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 26 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 27 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 28 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 29 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 30 URT/M17dQnrlJcQpfCBo0MEm8UY (comment: Evidence was from RNA-seq data) ------- COMMENT: dff943277843288b 31 5Ui1yzcWZm3mSQLS86kFr45Lajk (comment: Evidence form RNA-seq data) ------- COMMENT: dff943277843288b 32 5Ui1yzcWZm3mSQLS86kFr45Lajk (comment: Evidence form RNA-seq data) ------- COMMENT: dff943277843288b 33 5Ui1yzcWZm3mSQLS86kFr45Lajk (comment: Evidence form RNA-seq data) ------- COMMENT: dff943277843288b 34 5Ui1yzcWZm3mSQLS86kFr45Lajk (comment: Evidence form RNA-seq data) ------- COMMENT: dff943277843288b 35 5Ui1yzcWZm3mSQLS86kFr45Lajk (comment: Evidence form RNA-seq data) ------- COMMENT: dff943277843288b 36 5Ui1yzcWZm3mSQLS86kFr45Lajk (comment: Evidence form RNA-seq data) ------- COMMENT: dff943277843288b 37 5Ui1yzcWZm3mSQLS86kFr45Lajk (comment: Evidence form RNA-seq data) ------- COMMENT: dff943277843288b 38 5Ui1yzcWZm3mSQLS86kFr45Lajk (comment: Evidence form RNA-seq data) ------- COMMENT: dff943277843288b 39 5Ui1yzcWZm3mSQLS86kFr45Lajk (comment: Evidence form RNA-seq data) ------- COMMENT: dff943277843288b 40 5Ui1yzcWZm3mSQLS86kFr45Lajk (comment: Evidence form RNA-seq data) ------- COMMENT: dff943277843288b 41 5Ui1yzcWZm3mSQLS86kFr45Lajk (comment: Evidence form RNA-seq data) ------- COMMENT: dff943277843288b 42 5Ui1yzcWZm3mSQLS86kFr45Lajk (comment: Evidence form RNA-seq data) ------- COMMENT: dff943277843288b 43 9zu1E9lvvkTKmSJKnrYZRBdo+0Y (comment: Evidence from RNA-seq data) ------- COMMENT: dff943277843288b 44 9zu1E9lvvkTKmSJKnrYZRBdo+0Y (comment: Evidence from RNA-seq data) ------- COMMENT: dff943277843288b 45 9zu1E9lvvkTKmSJKnrYZRBdo+0Y (comment: Evidence from RNA-seq data) ------- COMMENT: dff943277843288b 46 9zu1E9lvvkTKmSJKnrYZRBdo+0Y (comment: Evidence from RNA-seq data) ------- COMMENT: dff943277843288b 47 9zu1E9lvvkTKmSJKnrYZRBdo+0Y (comment: Evidence from RNA-seq data) ------- COMMENT: dff943277843288b 48 9zu1E9lvvkTKmSJKnrYZRBdo+0Y (comment: Evidence from RNA-seq data) ------- COMMENT: dff943277843288b 49 9zu1E9lvvkTKmSJKnrYZRBdo+0Y (comment: Evidence from RNA-seq data) ------- COMMENT: dff943277843288b 50 9zu1E9lvvkTKmSJKnrYZRBdo+0Y (comment: Evidence from RNA-seq data) ------- COMMENT: dff943277843288b 51 9zu1E9lvvkTKmSJKnrYZRBdo+0Y (comment: Evidence from RNA-seq data) ------- COMMENT: dff943277843288b 52 9zu1E9lvvkTKmSJKnrYZRBdo+0Y (comment: Evidence from RNA-seq data) ------- COMMENT: dff943277843288b 53 9zu1E9lvvkTKmSJKnrYZRBdo+0Y (comment: Evidence from RNA-seq data) ------- COMMENT: dff943277843288b 54 9zu1E9lvvkTKmSJKnrYZRBdo+0Y (comment: Evidence from RNA-seq data) ------- COMMENT: dff943277843288b 55 9zu1E9lvvkTKmSJKnrYZRBdo+0Y (comment: Evidence from RNA-seq data) ------- COMMENT: dff943277843288b 56 9zu1E9lvvkTKmSJKnrYZRBdo+0Y (comment: Evidence from RNA-seq data) ------- COMMENT: dff943277843288b 57 9zu1E9lvvkTKmSJKnrYZRBdo+0Y (comment: Evidence from RNA-seq data) ------- COMMENT: dff943277843288b 58 9zu1E9lvvkTKmSJKnrYZRBdo+0Y (comment: Evidence from RNA-seq data) ------- COMMENT: e01b2f3ba979a8d0 1 V8aY9cMSuflWPDnZXK1Ej8D/EEk fig 1b ------- COMMENT: e01b2f3ba979a8d0 2 V8aY9cMSuflWPDnZXK1Ej8D/EEk fig 1b ------- COMMENT: e01b2f3ba979a8d0 3 V8aY9cMSuflWPDnZXK1Ej8D/EEk fig 1b ------- COMMENT: e01b2f3ba979a8d0 4 V8aY9cMSuflWPDnZXK1Ej8D/EEk fig 1b ------- COMMENT: e01b2f3ba979a8d0 5 V8aY9cMSuflWPDnZXK1Ej8D/EEk fig 1b ------- COMMENT: e01b2f3ba979a8d0 6 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: e01b2f3ba979a8d0 7 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: e01b2f3ba979a8d0 8 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: e01b2f3ba979a8d0 9 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: e01b2f3ba979a8d0 10 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: e01b2f3ba979a8d0 11 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: e01b2f3ba979a8d0 12 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: e01b2f3ba979a8d0 13 O966aea5XG1Cn/Wkw7Z0AnsiB9Q fig 2 (comment: CHECK synthetic rescue) ------- COMMENT: e01b2f3ba979a8d0 14 O966aea5XG1Cn/Wkw7Z0AnsiB9Q fig 2 (comment: CHECK synthetic rescue) ------- COMMENT: e01b2f3ba979a8d0 15 yyMNf1x4ofpAA8TPiE7bhUbV4SU fig 3b ------- COMMENT: e01b2f3ba979a8d0 16 yyMNf1x4ofpAA8TPiE7bhUbV4SU fig 3b ------- COMMENT: e01b2f3ba979a8d0 17 yyMNf1x4ofpAA8TPiE7bhUbV4SU fig 3b ------- COMMENT: e01b2f3ba979a8d0 18 fphwNxXv013sjtrPQI2L0d71dA4 fig 3b (comment: CHECK rescue) ------- COMMENT: e01b2f3ba979a8d0 19 fphwNxXv013sjtrPQI2L0d71dA4 fig 3b (comment: CHECK rescue) ------- COMMENT: e01b2f3ba979a8d0 20 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: e01b2f3ba979a8d0 21 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: e01b2f3ba979a8d0 22 J0aqpbBvwP2S79icCGoEZimYMvY fig 4a ------- COMMENT: e01b2f3ba979a8d0 23 HwHL0UToTQ8qu5C8qqOIlaSdQQc fig 4b-d ------- COMMENT: e01b2f3ba979a8d0 25 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: e01b2f3ba979a8d0 26 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: e01b2f3ba979a8d0 27 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: e01b2f3ba979a8d0 29 EV4Fuf4vWGtU1sao68buqRMkX4g (comment: poly...) ------- COMMENT: e01b2f3ba979a8d0 30 JvqKiF7syn97ZfVC9qlsi0ZVvfw fig9 ------- COMMENT: e01b2f3ba979a8d0 31 JvqKiF7syn97ZfVC9qlsi0ZVvfw fig9 ------- COMMENT: e01fd0fde5718e3f 2 ienwjKB/j3umVFDiHA2VpN1pRGQ Fig. 1f (comment: ATP-dependent) Supplementary Information, Movie 1) ------- COMMENT: e01fd0fde5718e3f 4 umtfEjFEgMoOIO8klisUmzKDahA (comment: CHECK homodimer) ------- COMMENT: e01fd0fde5718e3f 5 PF0/rezWsxQ4cOd2+QLqpqA4JMA Fig. 2c ------- COMMENT: e01fd0fde5718e3f 6 l06pGYJPxtnbXCsM/ZDUTb7eth8 Fig. 2d, lane 4 ------- COMMENT: e01fd0fde5718e3f 8 hzUwGmMKc2fb2gF6b5alNjOW3xk Fig. 2j ------- COMMENT: e01fd0fde5718e3f 9 hzUwGmMKc2fb2gF6b5alNjOW3xk Fig. 2j ------- COMMENT: e02ca2efe2171cda 1 fOgSGnRKmu0tZMJUsjbafTLeyTk (comment: localization dependent on actin cytoskeleton) ------- COMMENT: e038a0ad35ea7135 1 CFYxolcS6yzFhJkxY9Wx9XPhi7w Bundling activity inferred from pull-down experiments as well as from fluorescence microscopy ------- COMMENT: e040dc62fa860ce5 1 lDv9dhgQfmB8TDTPtJYnCTLN1uM In the array analysis, one of the most repressed genes in response to zinc deficiency was adh1, whereas one of the most highly expressed transcripts under this condition was an antisense transcript at this locus (Fig. 1A). ------- COMMENT: e040dc62fa860ce5 2 lDv9dhgQfmB8TDTPtJYnCTLN1uM In the array analysis, one of the most repressed genes in response to zinc deficiency was adh1, whereas one of the most highly expressed transcripts under this condition was an antisense transcript at this locus (Fig. 1A). ------- COMMENT: e040dc62fa860ce5 3 dQ9266LcEqKj2flTS9aN2Z1E0hE Taken together, the Northern and array analyses indicate that adh1AS transcripts preferentially accumulate in zinc-limited cells, whereas adh1 mRNAs accumulate in zinc-replete cells. ------- COMMENT: e040dc62fa860ce5 4 lDv9dhgQfmB8TDTPtJYnCTLN1uM In the array analysis, one of the most repressed genes in response to zinc deficiency was adh1, whereas one of the most highly expressed transcripts under this condition was an antisense transcript at this locus (Fig. 1A). ------- COMMENT: e040dc62fa860ce5 5 VGoUdhETQLUFBCAYtk/vgbxFOEI When adh1AS and adh1 transcript levels were examined in SPCC13B11.02c  cells, the adh1AS transcript was not detected, and adh1 mRNAs were detected in both zinc-limited and zinc-replete cells (Fig. 1C). ------- COMMENT: e040dc62fa860ce5 6 VGoUdhETQLUFBCAYtk/vgbxFOEI When adh1AS and adh1 transcript levels were examined in SPCC13B11.02c  cells, the adh1AS transcript was not detected, and adh1 mRNAs were detected in both zinc-limited and zinc-replete cells (Fig. 1C). ------- COMMENT: e040dc62fa860ce5 7 frkl5SjyGD60vItAh7CoU79dYCo However, the larger band preferentially accumulated under zinc-replete conditions in wild-type cells and constitutively accumulated in SPCC13B11.02c  cells. Thus, changes in adh1AS levels influence the levels of Adh1 protein, suggesting that this mechanism may exist to conserve zinc. ------- COMMENT: e04e3fecdc0c6d2b 1 oYlMtfHYQUTLioBkv4hcjXiZZXo figs 1, 4 ------- COMMENT: e04e3fecdc0c6d2b 2 oYlMtfHYQUTLioBkv4hcjXiZZXo figs 1, 4 ------- COMMENT: e04e3fecdc0c6d2b 3 oJ/VkTJh6uf6W7O++6g+u0RmgEw enriched at mat1 right border and cenH left border; fig 1 ------- COMMENT: e04e3fecdc0c6d2b 4 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: e04e3fecdc0c6d2b 5 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: e04e3fecdc0c6d2b 6 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: e04e3fecdc0c6d2b 7 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: e04e3fecdc0c6d2b 8 uU/wQm8EcGNfIFr1/OKlWDZ7xfk (comment: at MPS1) ------- COMMENT: e0b19b097d1d703d 4 rjovQZ4bjNoQr1qdmiOywD/vzno (comment: cross between h+ and h- deletions, allowed to sporulate immediately) ------- COMMENT: e0b19b097d1d703d 5 rjovQZ4bjNoQr1qdmiOywD/vzno (comment: cross between h+ and h- deletions, allowed to sporulate immediately) ------- COMMENT: e0b19b097d1d703d 6 rjovQZ4bjNoQr1qdmiOywD/vzno (comment: cross between h+ and h- deletions, allowed to sporulate immediately) ------- COMMENT: e0b19b097d1d703d 7 rjovQZ4bjNoQr1qdmiOywD/vzno (comment: cross between h+ and h- deletions, allowed to sporulate immediately) ------- COMMENT: e0f7d8e22ba14953 10 KICQAewL2vTaAo5qVKT4UPqrarg (comment: CHECK penetrance low if cells exposed to UV) ------- COMMENT: e0fb6894384ffb7a 1 6vjbkBWZBgTFdGQmQm6Wo/0LcCo Fig1A, 1B ------- COMMENT: e0fb6894384ffb7a 2 6vjbkBWZBgTFdGQmQm6Wo/0LcCo Fig1A, 1B ------- COMMENT: e0fb6894384ffb7a 3 6vjbkBWZBgTFdGQmQm6Wo/0LcCo Fig1A, 1B ------- COMMENT: e0fb6894384ffb7a 4 6vjbkBWZBgTFdGQmQm6Wo/0LcCo Fig1A, 1B ------- COMMENT: e0fb6894384ffb7a 5 6vjbkBWZBgTFdGQmQm6Wo/0LcCo Fig1A, 1B ------- COMMENT: e0fb6894384ffb7a 6 6vjbkBWZBgTFdGQmQm6Wo/0LcCo Fig1A, 1B ------- COMMENT: e0fb6894384ffb7a 7 6vjbkBWZBgTFdGQmQm6Wo/0LcCo Fig1A, 1B ------- COMMENT: e0fb6894384ffb7a 8 6vjbkBWZBgTFdGQmQm6Wo/0LcCo Fig1A, 1B ------- COMMENT: e0fb6894384ffb7a 9 6vjbkBWZBgTFdGQmQm6Wo/0LcCo Fig1A, 1B ------- COMMENT: e0fb6894384ffb7a 10 6vjbkBWZBgTFdGQmQm6Wo/0LcCo Fig1A, 1B ------- COMMENT: e0fb6894384ffb7a 11 6vjbkBWZBgTFdGQmQm6Wo/0LcCo Fig1A, 1B ------- COMMENT: e0fb6894384ffb7a 12 6vjbkBWZBgTFdGQmQm6Wo/0LcCo Fig1A, 1B ------- COMMENT: e0fb6894384ffb7a 13 6vjbkBWZBgTFdGQmQm6Wo/0LcCo Fig1A, 1B ------- COMMENT: e0fb6894384ffb7a 14 6vjbkBWZBgTFdGQmQm6Wo/0LcCo Fig1A, 1B ------- COMMENT: e0fb6894384ffb7a 15 n11lX6rIurbdSwiDWLbrLSmb5pY Fig1D ------- COMMENT: e0fb6894384ffb7a 16 3b3QSoBCKuA+mYiq+JcZzX6++1w Fig1D ------- COMMENT: e0fb6894384ffb7a 17 3b3QSoBCKuA+mYiq+JcZzX6++1w Fig1D ------- COMMENT: e0fb6894384ffb7a 18 3b3QSoBCKuA+mYiq+JcZzX6++1w Fig1D ------- COMMENT: e0fb6894384ffb7a 19 3b3QSoBCKuA+mYiq+JcZzX6++1w Fig1D ------- COMMENT: e0fb6894384ffb7a 20 3b3QSoBCKuA+mYiq+JcZzX6++1w Fig1D ------- COMMENT: e0fb6894384ffb7a 21 3b3QSoBCKuA+mYiq+JcZzX6++1w Fig1D ------- COMMENT: e0fb6894384ffb7a 22 3b3QSoBCKuA+mYiq+JcZzX6++1w Fig1D ------- COMMENT: e0fb6894384ffb7a 23 3b3QSoBCKuA+mYiq+JcZzX6++1w Fig1D ------- COMMENT: e0fb6894384ffb7a 24 3b3QSoBCKuA+mYiq+JcZzX6++1w Fig1D ------- COMMENT: e0fb6894384ffb7a 25 3b3QSoBCKuA+mYiq+JcZzX6++1w Fig1D ------- COMMENT: e0fb6894384ffb7a 26 3b3QSoBCKuA+mYiq+JcZzX6++1w Fig1D ------- COMMENT: e0fb6894384ffb7a 27 3b3QSoBCKuA+mYiq+JcZzX6++1w Fig1D ------- COMMENT: e0fb6894384ffb7a 28 3b3QSoBCKuA+mYiq+JcZzX6++1w Fig1D ------- COMMENT: e0fb6894384ffb7a 29 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: e0fb6894384ffb7a 30 b/W/YC3L0U5kOg0yP2N2gsIXCDA Fig1C ------- COMMENT: e0fb6894384ffb7a 31 b/W/YC3L0U5kOg0yP2N2gsIXCDA Fig1C ------- COMMENT: e0fb6894384ffb7a 32 5gWWTb9828wBx6jbC7A8HGT/Pws Fig2A, 2B ------- COMMENT: e0fb6894384ffb7a 33 5gWWTb9828wBx6jbC7A8HGT/Pws Fig2A, 2B ------- COMMENT: e0fb6894384ffb7a 34 W91AZLgGmNklfzTmva0cCQHCp0g FigS1C, S1D ------- COMMENT: e0fb6894384ffb7a 35 dLMc2lN+y4Ufz2MhLOHEIjs6B2A Fig2A ------- COMMENT: e0fb6894384ffb7a 36 JsCtrEE+qGyU9ltYXPA/sMGNAY0 Fig3A, 3B ------- COMMENT: e0fb6894384ffb7a 37 eT8JatHb8fQ7cK2SQP3Ka7Cr2z8 Fig3B, Table S4 mass spec used to show that there is bulk accumulation of nuclear localised protein rather than a few specific proteins ------- COMMENT: e0fb6894384ffb7a 38 XddPkb6OHehpChQKAi5I9hp12sg Fig2A, 2B ------- COMMENT: e0fb6894384ffb7a 39 oT/OcXiSsH6RWhVLJJEUtUifcUE Fig3A ------- COMMENT: e0fb6894384ffb7a 40 +lNO6gXlbvheQUHLB+/mKhhJuUQ Table S5 ------- COMMENT: e0fb6894384ffb7a 41 dLMc2lN+y4Ufz2MhLOHEIjs6B2A Fig2A ------- COMMENT: e0fb6894384ffb7a 42 dLMc2lN+y4Ufz2MhLOHEIjs6B2A Fig2A ------- COMMENT: e0fb6894384ffb7a 43 1QLmDgt0pgIwHkRnLwbWzTLFyWw Fig 2A ------- COMMENT: e0fb6894384ffb7a 44 yF9KJqdYmcIFxyIkvhE4FubEnn8 Fig4A, 4B ------- COMMENT: e0fb6894384ffb7a 45 yF9KJqdYmcIFxyIkvhE4FubEnn8 Fig4A, 4B ------- COMMENT: e0fb6894384ffb7a 46 RixlvlgTmMBOZ93/xdU305iyOZI Fig 4A ------- COMMENT: e0fb6894384ffb7a 47 8k4YSFTJD4UA6CiZCMg9PoJGFKQ Fig 4C, 4D ------- COMMENT: e0fb6894384ffb7a 49 y4zdnSJGbV/ZI7SHWWelf7p9Chg Fig 4c ------- COMMENT: e0fb6894384ffb7a 50 dvP2FEnAMolNvI9f77yTtMebGkI Fig4C (comment: cut6-621 partial suppresses the increased NC ratio of rae1-167 so not sure whether increased NC ration is the correct term) ------- COMMENT: e0fb6894384ffb7a 51 urOpmJ7V2cOTeJDX6yi2ZCNyVFA Fig 4D ------- COMMENT: e0fb6894384ffb7a 52 urOpmJ7V2cOTeJDX6yi2ZCNyVFA Fig 4D ------- COMMENT: e13e49a2df6b7722 9 ypwDHaIBnEpNTSa2YHzteqz6K08 fig5e ------- COMMENT: e13e49a2df6b7722 10 ypwDHaIBnEpNTSa2YHzteqz6K08 fig5e ------- COMMENT: e13e49a2df6b7722 15 pRANiKBKPjLvMhKt1F3lDg0ECow (comment: RECRUITS) ------- COMMENT: e183fe7f2ecde817 24 bJvXQ6W6cKoPIP+LBuPeWgzxSEs (comment: CHECK presence or absence of MMS) ------- COMMENT: e183fe7f2ecde817 31 2oBKBU8B1zEeYcEN8KZd4RTnoDw (comment: CHECK presence or absence of HU) ------- COMMENT: e183fe7f2ecde817 33 bJvXQ6W6cKoPIP+LBuPeWgzxSEs (comment: CHECK presence or absence of MMS) ------- COMMENT: e183fe7f2ecde817 37 bJvXQ6W6cKoPIP+LBuPeWgzxSEs (comment: CHECK presence or absence of MMS) ------- COMMENT: e183fe7f2ecde817 44 2oBKBU8B1zEeYcEN8KZd4RTnoDw (comment: CHECK presence or absence of HU) ------- COMMENT: e183fe7f2ecde817 45 Sk4Y4VjKXjj1Arpkww0FV8ZqT7U (comment: CHECK in presence of MMS) ------- COMMENT: e183fe7f2ecde817 46 xja69w/yKYl0JkgiI98vAnr6Ku0 (comment: CHECK in presence or absence of MMS) ------- COMMENT: e183fe7f2ecde817 47 xja69w/yKYl0JkgiI98vAnr6Ku0 (comment: CHECK in presence or absence of MMS) ------- COMMENT: e183fe7f2ecde817 56 xja69w/yKYl0JkgiI98vAnr6Ku0 (comment: CHECK in presence or absence of MMS) ------- COMMENT: e18451a565bca640 4 xUGNjLwJQzdSBqfj096B+s7O4m4 (Figure 1C, lane 8 ------- COMMENT: e18451a565bca640 5 umVh2un6W+Rru7wNvpsgVfS2tDc (Figure 2A). ------- COMMENT: e18451a565bca640 6 HbmNwNzRvJTcV+Qp9K7pJTaweXo (comment: SID PHENOTYPE) (Figure 2B). ------- COMMENT: e18451a565bca640 9 w0wKkAH6w7GsNsCMiOGd8acmq1c figure 2b ------- COMMENT: e18451a565bca640 13 HyQOeg8G3kIB/CDBf5YQ6DgvjFU figure 3biv ------- COMMENT: e18451a565bca640 14 vATwJOxfLYO2dgZ3DcgZ49LngNQ figure 3a ------- COMMENT: e18451a565bca640 15 vATwJOxfLYO2dgZ3DcgZ49LngNQ figure 3a ------- COMMENT: e18451a565bca640 16 vATwJOxfLYO2dgZ3DcgZ49LngNQ figure 3a ------- COMMENT: e18451a565bca640 17 HyQOeg8G3kIB/CDBf5YQ6DgvjFU figure 3biv ------- COMMENT: e18451a565bca640 18 q/HgBAKsW3WpN1CXjjFfcZ/mE+E table 2, par1/2 does not supress sin phenotype ------- COMMENT: e18451a565bca640 19 q/HgBAKsW3WpN1CXjjFfcZ/mE+E table 2, par1/2 does not supress sin phenotype ------- COMMENT: e18451a565bca640 20 q/HgBAKsW3WpN1CXjjFfcZ/mE+E table 2, par1/2 does not supress sin phenotype ------- COMMENT: e18451a565bca640 21 q/HgBAKsW3WpN1CXjjFfcZ/mE+E table 2, par1/2 does not supress sin phenotype ------- COMMENT: e18451a565bca640 22 q/HgBAKsW3WpN1CXjjFfcZ/mE+E table 2, par1/2 does not supress sin phenotype ------- COMMENT: e18451a565bca640 23 q/HgBAKsW3WpN1CXjjFfcZ/mE+E table 2, par1/2 does not supress sin phenotype ------- COMMENT: e18451a565bca640 24 q/HgBAKsW3WpN1CXjjFfcZ/mE+E table 2, par1/2 does not supress sin phenotype ------- COMMENT: e18451a565bca640 25 q/HgBAKsW3WpN1CXjjFfcZ/mE+E table 2, par1/2 does not supress sin phenotype ------- COMMENT: e18451a565bca640 26 q/HgBAKsW3WpN1CXjjFfcZ/mE+E table 2, par1/2 does not supress sin phenotype ------- COMMENT: e18451a565bca640 27 q/HgBAKsW3WpN1CXjjFfcZ/mE+E table 2, par1/2 does not supress sin phenotype ------- COMMENT: e18451a565bca640 28 q/HgBAKsW3WpN1CXjjFfcZ/mE+E table 2, par1/2 does not supress sin phenotype ------- COMMENT: e18451a565bca640 29 q/HgBAKsW3WpN1CXjjFfcZ/mE+E table 2, par1/2 does not supress sin phenotype ------- COMMENT: e18451a565bca640 30 2bqk5yEZ5qSuHrGhQ9dgsi0yInY Thefact that out of almost 200 tetrads analyzed, not a single ura  spore survived confirmed that spg1 is essential for vegetative growth, and this essential function cannot be bypassed by inactivating par1 and par2 by deletion. ------- COMMENT: e18451a565bca640 31 q7yQ4AbC2bEj6KiCpphVWV06cCo (comment: dns) ------- COMMENT: e18451a565bca640 32 UCtIUGrT9m9gm1WmjxyA3wHnAoI figure 4a ------- COMMENT: e18451a565bca640 33 UCtIUGrT9m9gm1WmjxyA3wHnAoI figure 4a ------- COMMENT: e18451a565bca640 34 OlLOeAlQ1NlpeSolWc1RnX7oxyc ------- COMMENT: e18451a565bca640 35 DFxXVrlJQmFpJ8ivuz0QFqGP/N0 (comment: asymetric localization is normal) ------- COMMENT: e1a1f6eb2da24846 1 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: e1a1f6eb2da24846 2 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: e1a1f6eb2da24846 3 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: e1a1f6eb2da24846 4 HBL6UR1SGUpWge6L5BoeOY2F8pA Fig. S1 ------- COMMENT: e1a1f6eb2da24846 8 XylehMZI/QPrJgW4wVaKCUR6tGg (comment: GFP-Cfh3) figure 1A ------- COMMENT: e1a1f6eb2da24846 9 XylehMZI/QPrJgW4wVaKCUR6tGg (comment: GFP-Cfh3) figure 1A ------- COMMENT: e1a1f6eb2da24846 10 UAo7gmGe1fE2/PqLg7rtMNrbP7c (comment: Cdc15-GFP) However, we observed that a number of the Cdc15-GFP and the GFP-Cdc4 rings were asymmetric or broken. ------- COMMENT: e1a1f6eb2da24846 11 Z/ZoDjl/VZ7wAGAwnw6O2++4mEc (comment: Cdc15-GFP) ------- COMMENT: e1a1f6eb2da24846 12 lkYbjfO2vbHWttHeyKS9tE/m2Xc (comment: Cdc15-GFP) supplemental figure S3 ------- COMMENT: e1a1f6eb2da24846 14 QDJEmMhJenvNjH9AEHLXgEA8CT0 (comment: GFP-Bgs1) ------- COMMENT: e1a1f6eb2da24846 15 QDJEmMhJenvNjH9AEHLXgEA8CT0 (comment: GFP-Bgs1) ------- COMMENT: e1a1f6eb2da24846 16 c89InDuyPBPk2VoM77srUS6PwKU figure S2, (comment: A protein distributed in cortex) ------- COMMENT: e1a1f6eb2da24846 17 2Fd1m7tYLkD986mpau9m26BnNiY Fig. 1B in which the SIN signal does not turn off, Cfh3p localized to the edge of the growing septa and it remained at the septal area after the septa had been completed. Thus, Cfh3p can arrive at the cell midzone in the absence of the SIN pathway but it requires that the SIN signal must be turned off for it to be removed from the cell equator after mitosis ------- COMMENT: e1a1f6eb2da24846 18 2Fd1m7tYLkD986mpau9m26BnNiY Fig. 1B in which the SIN signal does not turn off, Cfh3p localized to the edge of the growing septa and it remained at the septal area after the septa had been completed. Thus, Cfh3p can arrive at the cell midzone in the absence of the SIN pathway but it requires that the SIN signal must be turned off for it to be removed from the cell equator after mitosis ------- COMMENT: e1a1f6eb2da24846 19 NhkOKYbCh4Xk5344C0hpf+HgaEU Fig. 1B ------- COMMENT: e1a1f6eb2da24846 20 Fcvj+cqAON8FqgihxdppSKc/Scg (comment: used sorbitol but multiple stresses were tested) Cdc15-GFP However, we observed that a number of the Cdc15-GFP and the GFP-Cdc4 rings were asymmetric or broken. ------- COMMENT: e1b1536bc0389824 1 uFKoimRVuSLT5UgIsEqoY5Dl3h4 To confirm the binding of PA to the IDR, we performed a liposome-mediated binding assay. Liposomes with different percentages of PA were prepared and incubated with purified GFP-IDR protein. After isolation of the liposomes via density gradient centrifugation, the proteins bound to the liposomes were electrophoresed and detected using silver staining. GFP-IDR co-sedimented with PA-containing liposomes but not with control liposomes (Fig. 4B). Therefore, we conclude that the IDR region directly binds to PA in vitro. ------- COMMENT: e1b1536bc0389824 2 fbVUzXD43pZjvZQDCSu0ATIHRRc (comment: This region was identified as a lipid binding region.) ------- COMMENT: e1b1536bc0389824 3 uCGHgwVG8EdrqAKac83isRtA7Gg Figure 2 Under overexpression conditions, GFP-IDR was localized in the nucleoplasm, accompanied by a herniated structure adja- cent to the nucleus (Fig. 2E, arrows). ------- COMMENT: e1b1536bc0389824 4 5ciGMfcveiRzYaPG/MHkCc6EVf0 Upon shut-off of lem2, the cells expressing D1-140 and D259 to 383 fragments of Bqt4 did not grow, whereas the cells expressing D141 to 262 did (Fig. 1B) ------- COMMENT: e1b1536bc0389824 5 5ciGMfcveiRzYaPG/MHkCc6EVf0 Upon shut-off of lem2, the cells expressing D1-140 and D259 to 383 fragments of Bqt4 did not grow, whereas the cells expressing D141 to 262 did (Fig. 1B) ------- COMMENT: e1b1536bc0389824 6 5ciGMfcveiRzYaPG/MHkCc6EVf0 Upon shut-off of lem2, the cells expressing D1-140 and D259 to 383 fragments of Bqt4 did not grow, whereas the cells expressing D141 to 262 did (Fig. 1B) ------- COMMENT: e1b1536bc0389824 7 Bn65z+m3FAAkbT9eO8mR/qbD2BU All these fragments as well as wild-type Bqt4 (FL) were localized to the NE (Fig. 1C), suggesting that the lethality was not caused by the loss of NE localization. ------- COMMENT: e1b1536bc0389824 8 Bn65z+m3FAAkbT9eO8mR/qbD2BU All these fragments as well as wild-type Bqt4 (FL) were localized to the NE (Fig. 1C), suggesting that the lethality was not caused by the loss of NE localization. ------- COMMENT: e1b1536bc0389824 9 Bn65z+m3FAAkbT9eO8mR/qbD2BU All these fragments as well as wild-type Bqt4 (FL) were localized to the NE (Fig. 1C), suggesting that the lethality was not caused by the loss of NE localization. ------- COMMENT: e1b1536bc0389824 10 nackgUGhsxRsPU8UM8ozoQNwsUw The deletion mutants D340 to 383 and D364 to 383, but not D340 to 363, showed growth defect in the lem2-shut-off bqt4D strain (Fig. 1E), ------- COMMENT: e1b1536bc0389824 11 nackgUGhsxRsPU8UM8ozoQNwsUw The deletion mutants D340 to 383 and D364 to 383, but not D340 to 363, showed growth defect in the lem2-shut-off bqt4D strain (Fig. 1E), ------- COMMENT: e1b1536bc0389824 12 nackgUGhsxRsPU8UM8ozoQNwsUw The deletion mutants D340 to 383 and D364 to 383, but not D340 to 363, showed growth defect in the lem2-shut-off bqt4D strain (Fig. 1E), ------- COMMENT: e1b1536bc0389824 13 Bn65z+m3FAAkbT9eO8mR/qbD2BU All these fragments as well as wild-type Bqt4 (FL) were localized to the NE (Fig. 1C), suggesting that the lethality was not caused by the loss of NE localization. ------- COMMENT: e1b1536bc0389824 14 +vGZuhNaVn/9O+Hg34epCzLA9F8 and retained interaction with Bqt3 (Fig. S1B) ------- COMMENT: e1b1536bc0389824 15 gDV8WsHtffR8QWMY4UpuxQxak7g and the telomere anchoring (Fig. S1C). ------- COMMENT: e1b1536bc0389824 16 lzvE48CEJJscwLPOrzm+foSd7KE The DKSE mutant was expressed in lem2- shut-off bqt4D cells expressing GFP–GST–NLS, a nuclear protein marker, and nuclear protein leakage from the nucleus was assessed by calculating the fluorescence intensity ratio of the nucleus to the cytoplasm. Upon thiamine addition, GFP– GST–NLS severely leaked from the nucleus of DKSE mutant- expressing cells, but not from the nucleus of FL-expressing cells (Fig. 1, G and H), indicating NE rupture in the DKSE mutant-expressing cells. These results suggest that the KSE domain is vital for NE maintenance. ------- COMMENT: e1b1536bc0389824 18 GPLEjn5apwmtVvWBrHEQNe9Tqkw When GFP-IDR was overexpressed in S. pombe cells under the control of the nmt1 promoter, its overexpression resulted in severe growth defects in the spot assay (Fig. 2D). ------- COMMENT: e1b1536bc0389824 20 Y8gI+IjaiqNL1HiBl78gE+mOM9M Under overexpression conditions, GFP-IDR was localized in the nucleoplasm, accompanied by a herniated structure adja- cent to the nucleus (Fig. 2E, arrows). The Ish1 signal, which was localized in the NE under normal conditions, was distributed on cytoplasmic membranes besides the NE under overexpression conditions (Fig. 2E), suggesting that overexpression of GFP-IDR may cause abnormal mem- brane proliferation. ------- COMMENT: e1b1536bc0389824 21 3aQza7jBMz6dPIyouUOGvqlxc2M The Ish1 signal, which was localized in the NE under normal conditions, was distributed on cytoplasmic membranes besides the NE under overexpression conditions (Fig. 2E), ------- COMMENT: e1b1536bc0389824 22 K0kPS+/9dsMpLW/gVYAEXAD9WV8 Overexpression of the GFP-IDR and GFP-IDR-TM fragments, but not GFP and GFP-KSE, caused dissociation of the centromeres from the NE and their declustering (Fig. 3, A and B). ------- COMMENT: e1b1536bc0389824 23 e2uajNVPksErhNutOH3c2q5rZnA This dissociation did not affect transcriptional silencing in the centromeric region (Fig. S6A). ------- COMMENT: e1b1536bc0389824 24 qSgPo3khqwQA/pJDxxyxWv4ew1E Consequently, the chromosome was missegregated during the next mitosis (Fig. 3C).Alterna- tively, the entire chromosome moved to one side or the cells showed a cut phenotype (untimely torn cell phenotype); these cells displayed nuclear membrane extrusion along the spindle microtubule (Movie S2). ------- COMMENT: e1b1536bc0389824 25 5WHOaXxMbtp1woybgc5i0DdVV08 As predicted, the 4KRA mutant did not restore lethality upon shut-off, whereas the other three mutants did (Fig. 4C), ------- COMMENT: e1b1536bc0389824 26 5WHOaXxMbtp1woybgc5i0DdVV08 As predicted, the 4KRA mutant did not restore lethality upon shut-off, whereas the other three mutants did (Fig. 4C), ------- COMMENT: e1b1536bc0389824 27 5WHOaXxMbtp1woybgc5i0DdVV08 As predicted, the 4KRA mutant did not restore lethality upon shut-off, whereas the other three mutants did (Fig. 4C), ------- COMMENT: e1b1536bc0389824 28 5WHOaXxMbtp1woybgc5i0DdVV08 As predicted, the 4KRA mutant did not restore lethality upon shut-off, whereas the other three mutants did (Fig. 4C), ------- COMMENT: e1b1536bc0389824 29 YECRTlj7z056vMZ7dI3SM1ymLfM (comment: CHECK xxx NEW FYPO TERM nuclear lipid droplet formation XXXXXX.) As expected, the number of cells with nuclear LDs increased in the cells overexpressing Bqt4-FL (Fig. 6, C and D; 65.9%; see arrowhead in FL) compared to the control cells under the suppressive conditions (0–0.3%). ------- COMMENT: e1b1536bc0389824 30 4KuAfVkIxjAfSBjHiiDmUV4Wfi4 (comment: CHECK xxx NEW FYPO TERM nuclear lipid droplet formation XXXXXX.) Similar PA accumulation in the nucleus was observed in IDR-TM–over- expressing cells (IDR-TM in Figure 6, A and B for quantifica- tion). ------- COMMENT: e1b1536bc0389824 31 g68K6zPpoWQV+8+62aKa7cyxBpM (comment: CHECK xxx NEW FYPO TERM increased INM PA level XXXXXX.) ------- COMMENT: e1b1536bc0389824 32 g68K6zPpoWQV+8+62aKa7cyxBpM (comment: CHECK xxx NEW FYPO TERM increased INM PA level XXXXXX.) ------- COMMENT: e1b1536bc0389824 33 K0kPS+/9dsMpLW/gVYAEXAD9WV8 Overexpression of the GFP-IDR and GFP-IDR-TM fragments, but not GFP and GFP-KSE, caused dissociation of the centromeres from the NE and their declustering (Fig. 3, A and B). ------- COMMENT: e1b1536bc0389824 34 Bn65z+m3FAAkbT9eO8mR/qbD2BU All these fragments as well as wild-type Bqt4 (FL) were localized to the NE (Fig. 1C), suggesting that the lethality was not caused by the loss of NE localization. ------- COMMENT: e1b1536bc0389824 35 Bn65z+m3FAAkbT9eO8mR/qbD2BU All these fragments as well as wild-type Bqt4 (FL) were localized to the NE (Fig. 1C), suggesting that the lethality was not caused by the loss of NE localization. ------- COMMENT: e1c7a123d6b54783 1 cM/f8ArBjYGqnaElcI9u7LwRFm0 (comment: CHECK activated_by(CHEBI:16356)) ------- COMMENT: e1d7e0f4cb098e1f 1 Udy9IDpo6PsWQT4C1dP+NGALpR4 figure S1 ------- COMMENT: e1d7e0f4cb098e1f 2 Udy9IDpo6PsWQT4C1dP+NGALpR4 figure S1 ------- COMMENT: e1d7e0f4cb098e1f 3 EaCVhRRgQJ/GIuoH+ophtPwM3x4 figure S1 (comment: CHECK 20% longer) ------- COMMENT: e1d7e0f4cb098e1f 4 Udy9IDpo6PsWQT4C1dP+NGALpR4 figure S1 ------- COMMENT: e1d7e0f4cb098e1f 5 EaCVhRRgQJ/GIuoH+ophtPwM3x4 figure S1 (comment: CHECK 20% longer) ------- COMMENT: e1d7e0f4cb098e1f 6 EaCVhRRgQJ/GIuoH+ophtPwM3x4 figure S1 (comment: CHECK 20% longer) ------- COMMENT: e1d7e0f4cb098e1f 7 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: e1d7e0f4cb098e1f 8 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: e1d7e0f4cb098e1f 9 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: e1d7e0f4cb098e1f 10 Q0G+XgyLxrZiJUIfMzNZlJJpuUU Fig 1A ------- COMMENT: e1d7e0f4cb098e1f 11 9lYmhLaXwQmObFr9Gap4uun3jzE Fig 1C ------- COMMENT: e1d7e0f4cb098e1f 12 BO1GfkbcV+meMRUNCaYnYU1HxO8 (comment: DNs) ------- COMMENT: e1d7e0f4cb098e1f 13 PdwhseqlrW5Xuya8UXCRybLS6G0 (comment: DNS) ------- COMMENT: e1d7e0f4cb098e1f 14 QxyA7Kxt4u0SZftovXPYBddoxhU Fig 1D ------- COMMENT: e1d7e0f4cb098e1f 15 QxyA7Kxt4u0SZftovXPYBddoxhU Fig 1D ------- COMMENT: e1d7e0f4cb098e1f 16 sDA6jf/k9+xPKgzlhAE8KgfnesA fig2b ------- COMMENT: e1d7e0f4cb098e1f 17 sDA6jf/k9+xPKgzlhAE8KgfnesA fig2b ------- COMMENT: e1d7e0f4cb098e1f 18 j8lx0cjXOogtvtAy6trm2XWxJ9Q fig2a ------- COMMENT: e1d7e0f4cb098e1f 19 j8lx0cjXOogtvtAy6trm2XWxJ9Q fig2a ------- COMMENT: e1d7e0f4cb098e1f 20 9eIl2iWIJWroQ3R444DpXYYbDWk fig2d ------- COMMENT: e1d7e0f4cb098e1f 21 9eIl2iWIJWroQ3R444DpXYYbDWk fig2d ------- COMMENT: e1d7e0f4cb098e1f 26 lVvMgkFNoEp4aURnzoIpcy7J//0 fig 5A ------- COMMENT: e1d7e0f4cb098e1f 27 lVvMgkFNoEp4aURnzoIpcy7J//0 fig 5A ------- COMMENT: e1d7e0f4cb098e1f 28 lVvMgkFNoEp4aURnzoIpcy7J//0 fig 5A ------- COMMENT: e1d7e0f4cb098e1f 29 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: e1d7e0f4cb098e1f 30 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: e1d7e0f4cb098e1f 31 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: e1d7e0f4cb098e1f 32 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: e1d7e0f4cb098e1f 33 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: e1d7e0f4cb098e1f 34 VQwyyMBfQueRCgWSq5NNqBnXO6Q Figure 5D ------- COMMENT: e1d7e0f4cb098e1f 35 VQwyyMBfQueRCgWSq5NNqBnXO6Q Figure 5D ------- COMMENT: e1d7e0f4cb098e1f 38 VQwyyMBfQueRCgWSq5NNqBnXO6Q Figure 5D ------- COMMENT: e1d7e0f4cb098e1f 39 S8qforxCi6OCf2/391NxkiwnUT4 Figure 5D (comment: abolished pausing) ------- COMMENT: e1f04adb6bf32089 2 FGS0UaBEFsbS7vFQflcTyLEOGGc Tpz1-W498R,I501R disrupts interaction with Poz1 but retain interactions with Pot1 and Ccq1 based on co-IP experiments. In combination with ccq1 deletion, telomeres become unprotected and cells survive by circularizing chromosomes. Telomerase recruitment to telomeres is increased since Rad3/Tel1-dependent phosphorylation of Ccq1 Thr93, essential for promoting Ccq1-Est1 interaction and telomerase recruitment, is increased in tpz1-W498R,I501R cells. ------- COMMENT: e1f04adb6bf32089 5 If+69EYhEHQeUwWnjkBvi9bzUSg Tpz1-[1-485] disrupts interaction with Poz1 but retain interactions with Pot1 and Ccq1 based on co-IP experiments. In combination with ccq1 deletion, telomeres become unprotected and cells survive by circularizing chromosomes. Telomerase recruitment to telomeres is increased since Rad3/Tel1-dependent phosphorylation of Ccq1 Thr93, essential for promoting Ccq1-Est1 interaction and telomerase recruitment, is increased in tpz1-[1-485] cells. ------- COMMENT: e1f04adb6bf32089 8 PQYDpYF8qejophOwKiftHaIG140 Tpz1-L449R disrupts interaction with Ccq1 but retain interactions with Pot1 and Poz1 based on co-IP experiments. In combination with poz1 deletion, telomeres become unprotected and cells survive by circularizing chromosomes. Telomerase cannot be recruited to telomeres since Rad3/Tel1-dependent phosphorylation of Ccq1 Thr93, essential for promoting Ccq1-Est1 interaction and telomerase recruitment, is eliminated by tpz1-L449R. ------- COMMENT: e1f04adb6bf32089 11 JZtfAk0IAgJed9InxyItOyh1XG4 Tpz1-L439R,L445R disrupts interaction with Ccq1 but retain interactions with Pot1 and Poz1 based on co-IP experiments. ------- COMMENT: e1f4d0eca71f1467 4 JQX+7plDUWLyGk2LLqrn61jMjb8 Microscopic observa- tion showed that mitochondria were tubular in acb1+ cells but became fragmented in acb1Δ cells (Fig. 1A). ------- COMMENT: e1f4d0eca71f1467 5 gkkoe5IBpZCbKb8Xu/rD4MwRh9M We noticed that mito- chondrial fragmentation caused by the absence of Acb1 was more apparent when cells were cultured in nutrient- rich medium than in minimal medium. ------- COMMENT: e1f4d0eca71f1467 6 fsPftlFDU3zXvV80rf26+9zQxjM figure 1b, 3C ------- COMMENT: e1f4d0eca71f1467 7 x0owgmVy/mktaojU3C6+ua2mHvg Figure 1b, 3c ------- COMMENT: e1f4d0eca71f1467 8 C9TH031qaiyoOQvwxKl3gexjKoo As shown in Fig. 1B, acb1Δ grew slightly slower on fermentable med- ium than acb1+ cells and much slower on nonfer- mentable medium. ------- COMMENT: e1f4d0eca71f1467 9 0V+Cd6mSnTEUT7ZZBQG3TKxdRKA Consistent with the finding reported previously by our group [8], mitochondria formed a highly branch network (47.8%) in dnm1Δ cells (Fig. 2A,B) ------- COMMENT: e1f4d0eca71f1467 10 8Cdwt1h9RZHGCQ0kMjj/yctnIZw By con- trast, mitochondria became fragmented/aggregated (74.4%) PLUS This result indicates that mitochondrial mass/biogenesis was reduced by the absence of Acb1 or Dnm1. Note that dnm1-deletion in acb1Δ cells did not restore mitochondrial mass (Fig. 2C) ------- COMMENT: e1f4d0eca71f1467 11 TL3z4mhF1iNS5tqyxekbAqWLUD0 Figure 2a, 2b ------- COMMENT: e1f4d0eca71f1467 13 3a48idrR9E0q3q/MNgpN7koPDU8 (comment: phenotypoe seems to be additive on glycerol) Consistently, the growth of acb1Δ and acb1Δdnm1Δ cells on nonfer- mentable medium plates (YE plates plus 0.1% glucose and 3% glycerol) was comparable but was slower than the growth of WT and dnm1Δ cells (Fig. 2D,F). ------- COMMENT: e1f4d0eca71f1467 14 nZg9uk86XOOyloEYSfOHStYfzKk he expression of Dnm1 was comparable in wild-type and acb1Δ cells (Fig. 2E). ------- COMMENT: e1f4d0eca71f1467 15 J+cRE+X9ecqmhXlekNv/W+Yzek4 Three independent experiments consistently showed that the oxygen consumption rate of acb1Δ cells was significantly decreased when the cells were cultured in nutrient-rich medium (i.e. YE) (Fig. 3B). ------- COMMENT: e1f4d0eca71f1467 16 vePFt+MmeZ+5A59MhaZVCycI9xU Therefore, increased cell death rather than abnormal cell division was the conse- quence of the impaired cell proliferation caused by the absence of Acb1 (Fig. 3D). ------- COMMENT: e1f4d0eca71f1467 18 QiwQepQu42OXpEcGnSJW+NpBcgc Intriguingly, the size of lipid droplets became lar- ger and the number of lipid droplets became less as acb1+ and acb1Δ+acb1 cells grew older in nutrient- rich medium (Fig. 4C,D). By contrast, the size and number of lipid droplets did not change as acb1Δ cells grew older in nutrient-rich medium (Fig. 4C,D). ------- COMMENT: e1f4d0eca71f1467 19 vePFt+MmeZ+5A59MhaZVCycI9xU Therefore, increased cell death rather than abnormal cell division was the conse- quence of the impaired cell proliferation caused by the absence of Acb1 (Fig. 3D). ------- COMMENT: e1f4d0eca71f1467 20 eUkwlZ3xqzhyl6ZKYaD8oxmQdho figure 2c ------- COMMENT: e1f4d0eca71f1467 21 eUkwlZ3xqzhyl6ZKYaD8oxmQdho figure 2c ------- COMMENT: e1f4d0eca71f1467 22 eUkwlZ3xqzhyl6ZKYaD8oxmQdho figure 2c ------- COMMENT: e1f9774a7fd0a539 1 U/PvCZ3/sYCTc+VkXd+IsT2uQcI fig1b ------- COMMENT: e1f9774a7fd0a539 2 02l+3cqTh7UTFJSYjyKhzJabH68 fig1c ------- COMMENT: e1f9774a7fd0a539 3 02l+3cqTh7UTFJSYjyKhzJabH68 fig1c ------- COMMENT: e1f9774a7fd0a539 4 qzW3pb5ROo3kOBDAU8h+H7cAtRo fig1e (comment: twice their share of DNA and SPBs.) ------- COMMENT: e1f9774a7fd0a539 5 BAp+zLNeU1uUljIB2M69sH0rkRE fig1f ------- COMMENT: e1f9774a7fd0a539 6 s61o13YVaZ0tUVy0lO+Y7PfiMrI fig1B ------- COMMENT: e1f9774a7fd0a539 7 s61o13YVaZ0tUVy0lO+Y7PfiMrI fig1B ------- COMMENT: e1f9774a7fd0a539 8 s61o13YVaZ0tUVy0lO+Y7PfiMrI fig1B ------- COMMENT: e1f9774a7fd0a539 9 U/PvCZ3/sYCTc+VkXd+IsT2uQcI fig1b ------- COMMENT: e1f9774a7fd0a539 10 s61o13YVaZ0tUVy0lO+Y7PfiMrI fig1B ------- COMMENT: e1f9774a7fd0a539 11 s61o13YVaZ0tUVy0lO+Y7PfiMrI fig1B ------- COMMENT: e1f9774a7fd0a539 12 U/PvCZ3/sYCTc+VkXd+IsT2uQcI fig1b ------- COMMENT: e1f9774a7fd0a539 13 02l+3cqTh7UTFJSYjyKhzJabH68 fig1c ------- COMMENT: e1f9774a7fd0a539 14 s61o13YVaZ0tUVy0lO+Y7PfiMrI fig1B ------- COMMENT: e1f9774a7fd0a539 15 s61o13YVaZ0tUVy0lO+Y7PfiMrI fig1B ------- COMMENT: e1f9774a7fd0a539 16 s61o13YVaZ0tUVy0lO+Y7PfiMrI fig1B ------- COMMENT: e1f9774a7fd0a539 17 s61o13YVaZ0tUVy0lO+Y7PfiMrI fig1B ------- COMMENT: e1f9774a7fd0a539 20 jDW2DNDl5tnBTbprwm4YfmO0Lq0 Fig 1c ------- COMMENT: e1f9774a7fd0a539 21 jDW2DNDl5tnBTbprwm4YfmO0Lq0 Fig 1c ------- COMMENT: e1f9774a7fd0a539 22 jDW2DNDl5tnBTbprwm4YfmO0Lq0 Fig 1c ------- COMMENT: e1f9774a7fd0a539 23 2tN8jepmiJ4SHhRCOFXoyXjMN/Q In taz1Dlig4D zygotes, SPBs move normally during the horsetail stage even though they are rarely associated with chromatin. However, as the horsetail stage ends and meiosis I begins, the Pcp1-GFP signals appear brighter than in WT cells and are markedly disorganized (Figure 2B; 75 min ------- COMMENT: e1f9774a7fd0a539 27 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: e1f9774a7fd0a539 28 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: e1f9774a7fd0a539 29 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: e1f9774a7fd0a539 30 LI4jn9osmvZb7k9o6TsoBhIvkRo fig3 (comment: V-shaped patterns indicating multiple spindles) ------- COMMENT: e1f9774a7fd0a539 31 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: e1f9774a7fd0a539 32 NMooGwg2oln4ORjVb53Zjb/No84 fig3D ------- COMMENT: e1f9774a7fd0a539 33 7NgrCRGbJ6u+jsdmNrsoAGtYffY table S3 ------- COMMENT: e1f9774a7fd0a539 34 7NgrCRGbJ6u+jsdmNrsoAGtYffY table S3 ------- COMMENT: e1f9774a7fd0a539 35 7NgrCRGbJ6u+jsdmNrsoAGtYffY table S3 ------- COMMENT: e1f9774a7fd0a539 36 7NgrCRGbJ6u+jsdmNrsoAGtYffY table S3 ------- COMMENT: e1f9774a7fd0a539 37 N6gdfNLkuPfyqKNbJqqQxYyAr9g Fig. S3 ------- COMMENT: e1f9774a7fd0a539 38 N6gdfNLkuPfyqKNbJqqQxYyAr9g Fig. S3 ------- COMMENT: e1f9774a7fd0a539 39 N6gdfNLkuPfyqKNbJqqQxYyAr9g Fig. S3 ------- COMMENT: e1f9774a7fd0a539 40 N6gdfNLkuPfyqKNbJqqQxYyAr9g Fig. S3 ------- COMMENT: e1f9774a7fd0a539 41 N6gdfNLkuPfyqKNbJqqQxYyAr9g Fig. S3 ------- COMMENT: e1f9774a7fd0a539 42 N6gdfNLkuPfyqKNbJqqQxYyAr9g Fig. S3 ------- COMMENT: e1f9774a7fd0a539 43 N6gdfNLkuPfyqKNbJqqQxYyAr9g Fig. S3 ------- COMMENT: e1f9774a7fd0a539 44 qYkLkJz12cj0c2+M3nYSmiadNsM fig s4e, movie S2 ------- COMMENT: e1f9774a7fd0a539 45 /2nw5IE55ViJsqULLhZSilZIJ4M (comment: CHECK Matching synonym SPB detached from nucleuss fix syn) ------- COMMENT: e1f9774a7fd0a539 52 7NgrCRGbJ6u+jsdmNrsoAGtYffY table S3 ------- COMMENT: e1f9774a7fd0a539 55 Zl2cx0/+gWCfDGMH+fJNLBxxNtc fig3D (I) ------- COMMENT: e223a3074a0b59fa 4 RJJzPLiY5zsPd2tviTfQRSU+3rM (comment: of cell tip) ------- COMMENT: e254a152e54d321b 20 HIpcbLMjwWpkyF7M6VMMo9dVAvk (comment: cell size phenotype same as wee1 alone) ------- COMMENT: e254a152e54d321b 21 HIpcbLMjwWpkyF7M6VMMo9dVAvk (comment: cell size phenotype same as wee1 alone) ------- COMMENT: e2652e0c0cecb1f0 1 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: e2652e0c0cecb1f0 2 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: e28e479729269ce9 8 3dkg3/jSl69Q1p2ZBtNAIJpNuHQ Fig. S1B ------- COMMENT: e28e479729269ce9 9 3dkg3/jSl69Q1p2ZBtNAIJpNuHQ Fig. S1B ------- COMMENT: e28e479729269ce9 22 sGhekE4zhrWS/+P0z+XZ12K5C10 fig 6C ------- COMMENT: e28e479729269ce9 23 sGhekE4zhrWS/+P0z+XZ12K5C10 fig 6C ------- COMMENT: e28e479729269ce9 24 Za92JqfUsaM9smAsq86m4qG4NZ4 Our data suggest that Sad1 is present at the SPB early to set up structures that will trigger SPB inser- tion before the cell even enters mitosis.fig6 ------- COMMENT: e28e479729269ce9 33 +UxlsoIew0u4qLyQZzcQkl5Rca4 (comment: rename CHECK term https://github.com/geneontology/go-ontology/issues/14887) Our data suggest that Sad1 is present at the SPB early to set up structures that will trigger SPB inser- tion before the cell even enters mitosis.fig6 ------- COMMENT: e2d5c2adaa68d91d 6 EpE66EYJLV3W/ExH9F4XxKjAIxY (comment: CHECK strong phenotype = has_severity(FYPO_EXT:0000001)) ------- COMMENT: e2d5c2adaa68d91d 7 Ua/uL58gvL7tZ7ushok1muTcl0E The ght5 gene, the transcription of which is repressed in the WT cells, is transcribed at a high level in the presence of 111 mM glucose in the scr1 delta cells. ------- COMMENT: e2d5c2adaa68d91d 8 fmrOCs2xGcI1etFgVasWJeBrX+A growth defect of sds23 on low glucose MM is partially rescued by scr1 deletion ------- COMMENT: e2d5c2adaa68d91d 15 kCH4EZGvppZ8CQZu/0YMsf1NcEQ the level of ght5 transcription, which increases in the WT during glucose limitation, fails to increase in this mutant cells in low-glucose medium. ------- COMMENT: e2d5c2adaa68d91d 16 kCH4EZGvppZ8CQZu/0YMsf1NcEQ the level of ght5 transcription, which increases in the WT during glucose limitation, fails to increase in this mutant cells in low-glucose medium. ------- COMMENT: e2d5c2adaa68d91d 17 BG3c/oK4imWO4WWJMbW3NGQ7Wv0 The Ght5 protein, which is localized on the plasma membrane in the WT, fails to be localized on the plasma membrane, accumulating in the cytoplasm. ------- COMMENT: e2d5c2adaa68d91d 18 BG3c/oK4imWO4WWJMbW3NGQ7Wv0 The Ght5 protein, which is localized on the plasma membrane in the WT, fails to be localized on the plasma membrane, accumulating in the cytoplasm. ------- COMMENT: e2d5c2adaa68d91d 35 EpE66EYJLV3W/ExH9F4XxKjAIxY (comment: CHECK strong phenotype = has_severity(FYPO_EXT:0000001)) ------- COMMENT: e2d5c2adaa68d91d 36 /cAe/eYa3rMkiJpvEAMceRgzqIs (comment: CHECK during cellular response to glucose starvation) ------- COMMENT: e2d5c2adaa68d91d 37 /cAe/eYa3rMkiJpvEAMceRgzqIs (comment: CHECK during cellular response to glucose starvation) ------- COMMENT: e2d5c2adaa68d91d 38 /cAe/eYa3rMkiJpvEAMceRgzqIs (comment: CHECK during cellular response to glucose starvation) ------- COMMENT: e2d5c2adaa68d91d 39 /cAe/eYa3rMkiJpvEAMceRgzqIs (comment: CHECK during cellular response to glucose starvation) ------- COMMENT: e2d5c2adaa68d91d 55 BG3c/oK4imWO4WWJMbW3NGQ7Wv0 The Ght5 protein, which is localized on the plasma membrane in the WT, fails to be localized on the plasma membrane, accumulating in the cytoplasm. ------- COMMENT: e2d5c2adaa68d91d 56 BG3c/oK4imWO4WWJMbW3NGQ7Wv0 The Ght5 protein, which is localized on the plasma membrane in the WT, fails to be localized on the plasma membrane, accumulating in the cytoplasm. ------- COMMENT: e2d6e48248e1c105 2 dLT8cRYlRsj+MbcAwmOxrlTF14g (comment: RNA-seq) ------- COMMENT: e2d6e48248e1c105 4 dLT8cRYlRsj+MbcAwmOxrlTF14g (comment: RNA-seq) ------- COMMENT: e2d6e48248e1c105 5 IIKNlEg29kIJDQRZ/x0dy88BzpQ (comment: similar to pob3delta alone) ------- COMMENT: e2d6e48248e1c105 6 dLT8cRYlRsj+MbcAwmOxrlTF14g (comment: RNA-seq) ------- COMMENT: e2d6e48248e1c105 10 pRkbb7x5axHXmVQK1sZhjqkLARo (comment: assayed using bulk histones) ------- COMMENT: e2d6e48248e1c105 11 pRkbb7x5axHXmVQK1sZhjqkLARo (comment: assayed using bulk histones) ------- COMMENT: e2d6e48248e1c105 14 dLT8cRYlRsj+MbcAwmOxrlTF14g (comment: RNA-seq) ------- COMMENT: e2d6e48248e1c105 16 dLT8cRYlRsj+MbcAwmOxrlTF14g (comment: RNA-seq) ------- COMMENT: e2d6e48248e1c105 20 IIKNlEg29kIJDQRZ/x0dy88BzpQ (comment: similar to pob3delta alone) ------- COMMENT: e3188b7c6757274d 1 BowvK+o/ckLVllhyTOkMeuPxwlE deletion of loz1 leads to increased expression of this transcript in high zinc growth conditions (inferred from RNA seq analysis - see Table 1). ------- COMMENT: e3188b7c6757274d 3 BowvK+o/ckLVllhyTOkMeuPxwlE deletion of loz1 leads to increased expression of this transcript in high zinc growth conditions (inferred from RNA seq analysis - see Table 1). ------- COMMENT: e3188b7c6757274d 12 GN4QcUUiJx7+LCwKiwwkpJub6eA ChIP-seq, RNA-seq and northern blot analysis demonstrate that this transcript is repressed in high zinc in a manner that is dependent upon Loz1 (Table 1, Figure 3B and 3C) ------- COMMENT: e3188b7c6757274d 19 oPyP9VKHE+Mf8s4i2TZuFVBeoyE ChIP-seq, RNA-seq and reporter gene analysis demonstrate that this transcript is repressed in high zinc in a manner that is dependent upon Loz1 (Table 1, Figure 4) ------- COMMENT: e3188b7c6757274d 21 BowvK+o/ckLVllhyTOkMeuPxwlE deletion of loz1 leads to increased expression of this transcript in high zinc growth conditions (inferred from RNA seq analysis - see Table 1). ------- COMMENT: e3188b7c6757274d 23 GN4QcUUiJx7+LCwKiwwkpJub6eA ChIP-seq, RNA-seq and northern blot analysis demonstrate that this transcript is repressed in high zinc in a manner that is dependent upon Loz1 (Table 1, Figure 3B and 3C) ------- COMMENT: e3188b7c6757274d 25 GN4QcUUiJx7+LCwKiwwkpJub6eA ChIP-seq, RNA-seq and northern blot analysis demonstrate that this transcript is repressed in high zinc in a manner that is dependent upon Loz1 (Table 1, Figure 3B and 3C) ------- COMMENT: e3188b7c6757274d 26 GN4QcUUiJx7+LCwKiwwkpJub6eA ChIP-seq, RNA-seq and northern blot analysis demonstrate that this transcript is repressed in high zinc in a manner that is dependent upon Loz1 (Table 1, Figure 3B and 3C) ------- COMMENT: e3188b7c6757274d 27 GN4QcUUiJx7+LCwKiwwkpJub6eA ChIP-seq, RNA-seq and northern blot analysis demonstrate that this transcript is repressed in high zinc in a manner that is dependent upon Loz1 (Table 1, Figure 3B and 3C) ------- COMMENT: e3188b7c6757274d 28 GN4QcUUiJx7+LCwKiwwkpJub6eA ChIP-seq, RNA-seq and northern blot analysis demonstrate that this transcript is repressed in high zinc in a manner that is dependent upon Loz1 (Table 1, Figure 3B and 3C) ------- COMMENT: e3188b7c6757274d 30 IrBnf3rypuRwLbT+N2m0oxuW5g4 When Loz1 is expressed at a constant level inside of cells, it binds to the zrt1 promoter in high zinc conditions and not in low zinc conditions (comment: consistent with its role in gene repression in high zinc conditions) ------- COMMENT: e3188b7c6757274d 33 83JJaNI60gRKpFjqwI8BlUI5w7M (comment: CHECK ADD SO TERM WHEN AVAILABLE ) Mutagenesis of 3 Loz1 response elements in the SPBC1348.06c promoter resulted in the promoter no longer being repressed in high zinc in a manner that is dependent upon Loz1 (see Figure 4). The minimal Loz1 DNA binding domain (amino acids 426-522) also binds to this motif in vitro (supplemental Fig 2), and multiple copies of this element are able to confer Loz1-mediated gene repression in a minimal reporter system - see figure 6) ------- COMMENT: e3188b7c6757274d 35 7FtulUkfW7BvwQnXJwjWt9xSKQQ Loz1 represses gene expression when zinc is in excess and growth in zinc deficient media leads to de-repression of its target genes. Expression from the pgk1DTATA promoter leads to higher levels of Loz1 accumulating inside of cells, which in turn leads to higher levels of gene repression under low zinc conditions (Figure 1B) ------- COMMENT: e3188b7c6757274d 36 LkqpUW7piFvtxJQs8m/mHhpPvS0 When Loz1 is expressed at a constant level inside of cells, it binds to the adh4 promoter in high zinc conditions and not in low zinc conditions (consistent with its role in gene repression in high zinc conditions ------- COMMENT: e3b972dfd12d8605 1 cwnU9DJO/TB1ikPhAGuyfd+mPMs (comment: vw:added nucleosome assembly) ------- COMMENT: e3c8313e9bd1dabc 10 NSlOlnifJNw+HDqWgPRV/u2AqVs rec12 phenotype indicates that Sme2 role in synapsis is independent of meiotic recombination; (comment: NEEDS TO BE REGULATION OF SYNAPSISa change to the GO "pairing" definition requested"https://sourceforge.net/p/geneontology/ontology-requests/10607/) ------- COMMENT: e3f5a4777bcb5c84 1 Sv60khX7wrfe7hnBxqOGrIYs4FE fig1 (comment: cox4-GFP to label Mt) ------- COMMENT: e3f5a4777bcb5c84 2 fxJbLYRpMnz8yJzRZnbC65DB+QY fig. 1a ------- COMMENT: e3f5a4777bcb5c84 3 6qZKNYnRWWkYs/RN/pa4mjg3MWk fig. 1b (comment: n=344) ------- COMMENT: e3f5a4777bcb5c84 4 6qZKNYnRWWkYs/RN/pa4mjg3MWk fig. 1b (comment: n=344) ------- COMMENT: e3f5a4777bcb5c84 5 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: e3f5a4777bcb5c84 6 poJUXA1MoxbsUZwRwYFkjfVszR8 fig 1d. Despite the multiple types of abnormalities observed in emr1Δ cells, mitochondria of emr1Δ cells were still able to undergo fission and fusion (Fig. 1d), but improperly segregated into daughter cells after mitosis (Fig. 1e). This phenotype of defective mitochondrial segregation is consistent with the previous finding that spherical/giant mitochondria in mutant cells compromise mitochondrial movements, inheritance, and segregation7,8,2 ------- COMMENT: e3f5a4777bcb5c84 7 SESYGkC9ap7aLX1RRi2Nd60Y+F8 (comment: integral) Fig. 2a, 2b, 2c, 2d ------- COMMENT: e3f5a4777bcb5c84 9 vATwJOxfLYO2dgZ3DcgZ49LngNQ figure 3a ------- COMMENT: e3f5a4777bcb5c84 10 vATwJOxfLYO2dgZ3DcgZ49LngNQ figure 3a ------- COMMENT: e3f5a4777bcb5c84 11 RaPiJeE4LAzmUvVItFnpkPMvmGY figure 3b ------- COMMENT: e3f5a4777bcb5c84 12 3n1BoLj2EQ0xnu/x4IsErkzpwYI fig. 3b ------- COMMENT: e3f5a4777bcb5c84 13 RF1NL7BTMljAInHT1+mBTyAdZbU fig. 3d ------- COMMENT: e3f5a4777bcb5c84 14 pbGdHtjChubBcabXs32I9qoanGo fig 4 significantly decreased the number of Mdm12 (a constitutive component of the ERMES complex) foci ------- COMMENT: e3f5a4777bcb5c84 15 r7HHCI1mclYw5iZFJT0xKyAVcE8 fig 4d (comment: though the expression levels of Mdm12 were comparable in WT and emr1Δ Cells) ------- COMMENT: e3f5a4777bcb5c84 16 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: e3f5a4777bcb5c84 21 wQ5ShviHoc2l0AavQ8ARMEroo/A As shown in Fig. 6b, c, Emr1-FL and Emr1-ΔN, but not Emr1-ΔC, restored the normal number of Mdm12 foci, confirming that the C-terminus of Emr1 is required for regulating the number of ERMES foci. ------- COMMENT: e3f5a4777bcb5c84 26 KzvBhA+XMoNbzCHMc5I/giBP1kQ (comment: er to mitochondria) ------- COMMENT: e45f4e0f49c95352 4 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: e45f4e0f49c95352 5 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: e45f4e0f49c95352 6 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: e45f4e0f49c95352 7 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: e45f4e0f49c95352 8 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: e45f4e0f49c95352 9 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: e45f4e0f49c95352 10 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: e45f4e0f49c95352 11 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: e45f4e0f49c95352 12 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: e479cd586b7c6c9b 1 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: e479cd586b7c6c9b 2 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: e479cd586b7c6c9b 3 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: e479cd586b7c6c9b 4 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: e479cd586b7c6c9b 5 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: e4b31ea722f9e274 9 1PHC+mog6672vciFmpdtHimC7Wg Telomere length rescue by overexpression of Pfh1 ------- COMMENT: e583692a60c79c22 40 cWI+RTvdFdOXNQvTknINdoEKhQ8 the basal level of the 3.2 kb transcript was lower than that in h90 wild type cells, but the 3 kb transcript was properly induced upon nitrogen starvation ------- COMMENT: e583692a60c79c22 42 c7zi2hm73lByh+X/hqDXaT9VXxE (comment: 3 kb transcript) ------- COMMENT: e58982138a6526c3 10 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: e58982138a6526c3 11 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: e58982138a6526c3 12 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: e58982138a6526c3 13 w/pLOXz6HRJR0VKEC+LxuYSbXRM fig 1D, 1E (comment: CHECK ~55%) ------- COMMENT: e58982138a6526c3 14 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: e58982138a6526c3 15 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: e58982138a6526c3 16 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: e58982138a6526c3 17 lEHvwaeTUDVD7XwpLmOHHP9EAcQ (comment: CHeCK phenotypes) ------- COMMENT: e58982138a6526c3 18 gbsEZDL2WsTbMBJ8nTkh0vGNYlA fig 2A ------- COMMENT: e58982138a6526c3 19 gbsEZDL2WsTbMBJ8nTkh0vGNYlA fig 2A ------- COMMENT: e58982138a6526c3 20 iyMraj5qmLEJtewo/fSEFxmllqQ figure 2D ------- COMMENT: e58982138a6526c3 21 gbsEZDL2WsTbMBJ8nTkh0vGNYlA fig 2A ------- COMMENT: e58982138a6526c3 22 gbsEZDL2WsTbMBJ8nTkh0vGNYlA fig 2A ------- COMMENT: e58982138a6526c3 23 gbsEZDL2WsTbMBJ8nTkh0vGNYlA fig 2A ------- COMMENT: e58982138a6526c3 24 gbsEZDL2WsTbMBJ8nTkh0vGNYlA fig 2A ------- COMMENT: e58982138a6526c3 25 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: e58982138a6526c3 26 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: e58982138a6526c3 27 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: e58982138a6526c3 28 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: e58982138a6526c3 29 gbsEZDL2WsTbMBJ8nTkh0vGNYlA fig 2A ------- COMMENT: e58982138a6526c3 30 cWU1gxrBhSdpW25jqWFTdTZ9qBA fig 3A ------- COMMENT: e58982138a6526c3 31 od+EBxKErbrxOh+KczJ9KOGsCUk fig 3B ------- COMMENT: e58982138a6526c3 32 23A7gKYH++mwxpDaLPxFOcAYx6Y fig 3c ------- COMMENT: e58982138a6526c3 33 qfCpyd4bXuEZVVk93nKURYdKtTo fig S3A ------- COMMENT: e58982138a6526c3 34 yn5/s6S+P83NU/sZm1yJpcjeWbk fig 3D ------- COMMENT: e58982138a6526c3 35 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: e58982138a6526c3 36 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: e58982138a6526c3 37 nFFJT2Q6/N+nA3J1BrAqrMQ8FLU Figure S3F ------- COMMENT: e58982138a6526c3 38 nFFJT2Q6/N+nA3J1BrAqrMQ8FLU Figure S3F ------- COMMENT: e58982138a6526c3 39 nFFJT2Q6/N+nA3J1BrAqrMQ8FLU Figure S3F ------- COMMENT: e58982138a6526c3 40 nFFJT2Q6/N+nA3J1BrAqrMQ8FLU Figure S3F ------- COMMENT: e58982138a6526c3 41 nFFJT2Q6/N+nA3J1BrAqrMQ8FLU Figure S3F ------- COMMENT: e58982138a6526c3 42 nFFJT2Q6/N+nA3J1BrAqrMQ8FLU Figure S3F ------- COMMENT: e58982138a6526c3 43 8zdno63XFs3BFsVP161LRtfCMNM Figure 3D ------- COMMENT: e58982138a6526c3 44 yn5/s6S+P83NU/sZm1yJpcjeWbk fig 3D ------- COMMENT: e58982138a6526c3 45 rkK5HvLqUdB3hrv1NJJNG41QYTM fig 3h ------- COMMENT: e58982138a6526c3 46 3y9oq6+EI38xkopiy7PU8NuqM1I Figure 3H ------- COMMENT: e58982138a6526c3 47 FnsDIpqq0fiCBBbREGZxJIkdWDQ Fig 4C, 4D ------- COMMENT: e58982138a6526c3 48 FnsDIpqq0fiCBBbREGZxJIkdWDQ Fig 4C, 4D ------- COMMENT: e58982138a6526c3 49 8W5qxA5m7DdxqONqx7Ruu/PEVcA figure S4C ------- COMMENT: e58982138a6526c3 50 8W5qxA5m7DdxqONqx7Ruu/PEVcA figure S4C ------- COMMENT: e58982138a6526c3 51 8W5qxA5m7DdxqONqx7Ruu/PEVcA figure S4C ------- COMMENT: e58982138a6526c3 52 8W5qxA5m7DdxqONqx7Ruu/PEVcA figure S4C ------- COMMENT: e58982138a6526c3 53 0aFtDz4AWYlibvHdCDDq3LR3jPs Fig 4F ------- COMMENT: e58982138a6526c3 54 lzdFYksYyF15l9Q/d/mcNLA5/Pk Fig 4F (comment: lasso) ------- COMMENT: e5b469b55d5c3cba 32 VOMp6+prWIuXZNrFlTXLSHFSBWk (comment: same as rqh1delta alone) ------- COMMENT: e5b469b55d5c3cba 33 UgwUJ85cfsTRkeF8OHxXDHqt0Hg (comment: same as cds1delta alone) ------- COMMENT: e5b469b55d5c3cba 35 mbrWqZOO5jVP7gmwCvYuih5j7q8 (comment: same as cdc27-P11 alone) ------- COMMENT: e5d482e80f83526c 2 YTlT6x7rr6WqomejwvPEoIqZjK0 Figure 3B Some cells appeared abnormally round, curved, bulged, or branched, indicating that Nup211 plays a role in maintaining proper cell shape (Fig 3B). Interestingly, nup211 shut-off cells also showed severe defects in septation and cytokine- sis. These defects varied widely: some cells failed to develop a septum during division (Fig 3B- a), while others developed thicker (Fig 3B-b), misplaced (Fig 3B-c), or multiple septa (Fig 3B- d). Furthermore, septa were sometimes seen in shorter cells (Fig 3B-c), while other phenotypes like bulging (Fig 3B-c, 3B-e), branching (Fig 3B-e), curving, and swelling (Fig 3B-f) were also observed. ------- COMMENT: e5d482e80f83526c 4 nSJbK7aOZObOCbgG7eA8m3sB6Zc Fig 7. Nup211 regulates the expression of several genes involved in cytokinesis. ------- COMMENT: e5d482e80f83526c 5 nSJbK7aOZObOCbgG7eA8m3sB6Zc Fig 7. Nup211 regulates the expression of several genes involved in cytokinesis. ------- COMMENT: e5d482e80f83526c 6 nSJbK7aOZObOCbgG7eA8m3sB6Zc Fig 7. Nup211 regulates the expression of several genes involved in cytokinesis. ------- COMMENT: e5d482e80f83526c 7 nSJbK7aOZObOCbgG7eA8m3sB6Zc Fig 7. Nup211 regulates the expression of several genes involved in cytokinesis. ------- COMMENT: e5d482e80f83526c 8 nSJbK7aOZObOCbgG7eA8m3sB6Zc Fig 7. Nup211 regulates the expression of several genes involved in cytokinesis. ------- COMMENT: e5d482e80f83526c 9 nSJbK7aOZObOCbgG7eA8m3sB6Zc Fig 7. Nup211 regulates the expression of several genes involved in cytokinesis. ------- COMMENT: e5d482e80f83526c 10 nSJbK7aOZObOCbgG7eA8m3sB6Zc Fig 7. Nup211 regulates the expression of several genes involved in cytokinesis. ------- COMMENT: e5d482e80f83526c 11 nSJbK7aOZObOCbgG7eA8m3sB6Zc Fig 7. Nup211 regulates the expression of several genes involved in cytokinesis. ------- COMMENT: e5d482e80f83526c 12 nSJbK7aOZObOCbgG7eA8m3sB6Zc Fig 7. Nup211 regulates the expression of several genes involved in cytokinesis. ------- COMMENT: e5d482e80f83526c 13 crdk8+5Su6ZjOoDfvYBv9bmPLs0 Figure 5C ------- COMMENT: e5d482e80f83526c 15 WW5P1eMp7HwjIheW/F9qD41OWa0 Fig 4A ------- COMMENT: e5d482e80f83526c 16 R+u4JenWUeRMPcjsqbolGKk6CuA Exogenous expression of Nup211, Nup2111-863, or Nup2111-655 restored cell viability; however, expression of Nup2111-1033 only led to a partial recovery (Fig 4A) ------- COMMENT: e5d482e80f83526c 18 1K+BotSAjARLO5EL0LJZH7PDKkg Compared with wild type cells, a higher percentage of nup211 shut-off cells contained multiple and/or thicker septa (Fig 3C). ------- COMMENT: e5d482e80f83526c 19 UWUxfGF9FCojI7phfcP/cvlutXU some cells failed to develop a septum during division (Fig 3B- a), ------- COMMENT: e5d482e80f83526c 20 R+u4JenWUeRMPcjsqbolGKk6CuA Exogenous expression of Nup211, Nup2111-863, or Nup2111-655 restored cell viability; however, expression of Nup2111-1033 only led to a partial recovery (Fig 4A) ------- COMMENT: e5f8e6c0322f4204 4 KMkKZYd8PbS5ODPaPw9zjVM4qvs (comment: CHECK activated by ATP) ------- COMMENT: e5f8e6c0322f4204 5 KMkKZYd8PbS5ODPaPw9zjVM4qvs (comment: CHECK activated by ATP) ------- COMMENT: e5f8e6c0322f4204 6 KMkKZYd8PbS5ODPaPw9zjVM4qvs (comment: CHECK activated by ATP) ------- COMMENT: e62b222855e26bac 35 g2x7j8BGnWXdKzVOpg0nIvwQsVw (comment: dependent_on(GO:0006312)| not_dependent_on(GO:0007004)) ------- COMMENT: e630ab034081e15c 6 LJZL9UGUTfOmEp0wpsddjOgZiHk (comment: CFU counts) ------- COMMENT: e64102699ced97dc 15 cPkiWU2UQWwu72pXNUr4Vxw2ADc (comment: CHECK SO:0000286 = LTR) ------- COMMENT: e64102699ced97dc 17 cPkiWU2UQWwu72pXNUr4Vxw2ADc (comment: CHECK SO:0000286 = LTR) ------- COMMENT: e64102699ced97dc 35 v0AMcg71cSuWg7gXSqqwqq5469A (comment: independent of Clr4) ------- COMMENT: e64102699ced97dc 36 v0AMcg71cSuWg7gXSqqwqq5469A (comment: independent of Clr4) ------- COMMENT: e64102699ced97dc 40 v0AMcg71cSuWg7gXSqqwqq5469A (comment: independent of Clr4) ------- COMMENT: e64d08d70519aefd 1 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: e64d08d70519aefd 2 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: e64d08d70519aefd 3 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: e64d08d70519aefd 4 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: e64d08d70519aefd 5 0yWJCGN3F+XGdfp/lfzvPJbD2+I Figure 2A In contrast, both of the single mutants were hypersensitive to this concentration of TBZ, showing a 5- to 25-fold growth reduction compared with wt ------- COMMENT: e64d08d70519aefd 6 0yWJCGN3F+XGdfp/lfzvPJbD2+I Figure 2A In contrast, both of the single mutants were hypersensitive to this concentration of TBZ, showing a 5- to 25-fold growth reduction compared with wt ------- COMMENT: e64d08d70519aefd 7 0yWJCGN3F+XGdfp/lfzvPJbD2+I Figure 2A In contrast, both of the single mutants were hypersensitive to this concentration of TBZ, showing a 5- to 25-fold growth reduction compared with wt ------- COMMENT: e64d08d70519aefd 8 0yWJCGN3F+XGdfp/lfzvPJbD2+I Figure 2A In contrast, both of the single mutants were hypersensitive to this concentration of TBZ, showing a 5- to 25-fold growth reduction compared with wt ------- COMMENT: e64d08d70519aefd 9 igJNmDg8VeOpx8fxhYX7Ho928v0 Figure 2 In cultures without TSA, the hrp1D cells grew slightly faster than wt cells as reported previously (48). ------- COMMENT: e64d08d70519aefd 10 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: e64d08d70519aefd 11 0yWJCGN3F+XGdfp/lfzvPJbD2+I Figure 2A In contrast, both of the single mutants were hypersensitive to this concentration of TBZ, showing a 5- to 25-fold growth reduction compared with wt ------- COMMENT: e64d08d70519aefd 12 mkcBdWwWIjK7ep/BhEaZzyBs7VI Figure 2A However, growth of the double mutant cells was completely inhibited by TSA. ------- COMMENT: e64d08d70519aefd 13 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: e64d08d70519aefd 14 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: e64d08d70519aefd 15 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: e64d08d70519aefd 16 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: e64d08d70519aefd 17 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: e64d08d70519aefd 18 GTLpwwBFd/JjO6ipt7LWZ6GY7uo Figure 2 Further examination of the IF samples revealed that hrp1D single and hrp1D hrp3D double mutants cells showed elevated numbers of asymmetric segregation (large and small nuclei) in late anaphase cells ------- COMMENT: e64d08d70519aefd 20 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: e64d08d70519aefd 21 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: e64d08d70519aefd 22 Bk7Xzu3XBVAnzwkQmlqif8/kZSc Figure 3 There was a 4- fold reduction of Cnp1 at cnt2 in hrp1D cells, ------- COMMENT: e64d08d70519aefd 23 Bk7Xzu3XBVAnzwkQmlqif8/kZSc Figure 3 There was a 4- fold reduction of Cnp1 at cnt2 in hrp1D cells, ------- COMMENT: e64d08d70519aefd 24 caS7ZOfkYZm/ItaYG4rwM2XH1fw The mis6-302 hrp1D double mutant had a reduced growth at 30 C as compared with the mis6-302 and hrp1D single mutants (Figure 3C). ------- COMMENT: e64d08d70519aefd 25 kbJyYYPc+kbSaOSi3VEO+7ud/NU dhIII transcripts were detectable in hrp1D dcr1D and dcr1D cells, but not in the wild-type and hrp1D cells (Figure 4E). The dhIII transcripts were more abundant in hrp1D dcr1D cells than in dcr1D cells consistent with the reduced silencing observed at dg-dh in hrp1D (Figure 1E). If transcripts read through in hrp1D from dh-dg into the central core region, then they should be readily detectable in the intervening imrIII region. However, imrIII transcripts were not observed in hrp1D cells. From these results, we concluded that the hrp1D mutant does not cause read through of dg-dh transcripts into the central core region. Hrp1 is present at the centromere in a cell ------- COMMENT: e66906b54c5398a2 8 9xN097BflJV3+Fn7qa+2NP5MTsE (comment: CHECK persistence of the meiosis I spindle after the assembly of the meiosis II spindle) ------- COMMENT: e677bdbfedf4a422 1 iAx2YYYS9enisrUdMuR90de6pvg (comment: Promotes cell wall thickness hoemostasis) ------- COMMENT: e677bdbfedf4a422 2 iAx2YYYS9enisrUdMuR90de6pvg (comment: Promotes cell wall thickness hoemostasis) ------- COMMENT: e677bdbfedf4a422 3 iAx2YYYS9enisrUdMuR90de6pvg (comment: Promotes cell wall thickness hoemostasis) ------- COMMENT: e677bdbfedf4a422 4 g9KhOcD2Iw2cOBpQvzTeWZG5jNQ figure 2F ------- COMMENT: e677bdbfedf4a422 5 8F8k7RN2g6ngxsyw43muHmOOOzA figure 3B ------- COMMENT: e677bdbfedf4a422 6 8F8k7RN2g6ngxsyw43muHmOOOzA figure 3B ------- COMMENT: e677bdbfedf4a422 7 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: e677bdbfedf4a422 8 tqyYa/JZQyx+3vRzrh9CYJL/3uM (comment: thicker and thinner, disrupted homeostasis) ------- COMMENT: e677bdbfedf4a422 9 tqyYa/JZQyx+3vRzrh9CYJL/3uM (comment: thicker and thinner, disrupted homeostasis) ------- COMMENT: e677bdbfedf4a422 10 tqyYa/JZQyx+3vRzrh9CYJL/3uM (comment: thicker and thinner, disrupted homeostasis) ------- COMMENT: e68d23abf86a3c7c 1 wVkz5iierGK7pB9ucDLnNJ/WUgQ (comment: in vivo phosphorylation sites identified by mass spectrometry) ------- COMMENT: e68d23abf86a3c7c 2 Vz0GP0TETtgy8qlF6iTT+gPRYYo (comment: in vivo phosphorylation site identified by mass spectrometry) ------- COMMENT: e68d23abf86a3c7c 9 lGKJxGnovyzydFBo0CND/6P3TLY (comment: CHECK in vitro kinase assay showed S166 is phosphorylated by Cdk1) ------- COMMENT: e68d23abf86a3c7c 10 7a5glKlrtfQG1olxYYCxg1TFsjk (comment: CHECK in vitro kinase assay showed S251 is phosphorylated by Plo1) ------- COMMENT: e68d23abf86a3c7c 11 2sfT2C6jknx6mud9YCWo7zXCHwE (comment: CHECK in vitro kinase assay showed T18, S20, and S266 are phosphorylated by CK2) ------- COMMENT: e68d23abf86a3c7c 13 VHm3ue5jdPiwG1V3fc6HNf2a9YI Although this assay is not quantitative, we found that Sid4 was ubiquitinated to similar levels as in wild-type in both dma1-6A and dma1-6D/E but was not ubiquitinated in dma1D (Fig. 2A). ------- COMMENT: e68d23abf86a3c7c 16 +7fPpLIiAo6zQkpN7JfKKA/o3rw (comment: CHECK in vitro assay) ------- COMMENT: e68d23abf86a3c7c 17 mkjdrHoq+Lu4d8S3HKpz04Yn1i8 (comment: CHECK Decreased Dma1 auto-ubiquitination by in vitro assay) ------- COMMENT: e68d23abf86a3c7c 18 stjCUrw92iiCLkYyzZb+23hwHHk Fig. 2B). (comment: CHECK Almost abolished Dma1 auto-ubiquitination by in vitro assay) ------- COMMENT: e68d23abf86a3c7c 19 wvOsy87YrjNymDyFBWVDgqbEi9w (Fig.2B). Dma1-6D/E auto-ubiquitination was modestly but reproducibly reduced relative to wild- type. Specifically, while 82% of wild-type Dma1 became ubiquitinated on at least one site, 71% of Dma1-6D/E did. ------- COMMENT: e68d23abf86a3c7c 21 cExrvqvhPg+cAyM3hUp3QrcevmI (Fig.3A–C). Reduced contractile ring localization during mitosis. However, we observed that Dma1-6A was significantly more difficult to detect at the first instance of CR localization early in mitosis than either Dma1 or Dma1-6D/E ------- COMMENT: e68d23abf86a3c7c 25 4JgFntWIBARDSxY7Uj4DUtqzTcQ Normal localization to medial cortical nodes, SPB, and division septum as wildtype ------- COMMENT: e68d23abf86a3c7c 26 bSu36AZwXY0gvM/JKzaXMEjs32U Normal localization to medial cortical nodes, mitotic contractile ring, SPB, and septum as wildtype ------- COMMENT: e68d23abf86a3c7c 27 ZUr6czb5V6GgHMktM9GfjO0MlrQ Fig 4a We observed that nda3-km311, dma1- 6A nda3-km311, and dma1-6D/E nda3-km311 cells delayed septation relative to wild-type cells and that dma1Δ cells did not ------- COMMENT: e68d23abf86a3c7c 28 YBsaO0Gzy5Iavf8E+ye0FUTjroc Fig 4a ------- COMMENT: e68d23abf86a3c7c 30 LsgITssNetvDM7VBSdGiZBPIUY8 Localization to SPBs at the same level as wildtype during spindle stress ------- COMMENT: e68d23abf86a3c7c 31 LsgITssNetvDM7VBSdGiZBPIUY8 Localization to SPBs at the same level as wildtype during spindle stress ------- COMMENT: e68d23abf86a3c7c 33 31RpnuYWUTy5NCoBZa2iqbK72ZY (comment: CHECK ALLELELS) we combined analog-sensitive (cdc2-as, orb5-as) and temperature- sensitive (plo1-1) alleles. Despite these kinases targeting Dma1 in vitro, we found no evidence that inhibiting any of them singly (not shown) or together (Fig. 1G) chan- ged Dma1 phosphostatus as monitored by SDS/PAGE mobility suggesting that these kinases are not responsible for regulating Dma1 phosphostatus in cells. ------- COMMENT: e68d23abf86a3c7c 34 4JgFntWIBARDSxY7Uj4DUtqzTcQ Normal localization to medial cortical nodes, SPB, and division septum as wildtype ------- COMMENT: e68d23abf86a3c7c 35 2sfT2C6jknx6mud9YCWo7zXCHwE (comment: CHECK in vitro kinase assay showed T18, S20, and S266 are phosphorylated by CK2) ------- COMMENT: e69ef00f0c3516d9 7 BmvIiPrON84o88TzaI3fhPz+Fu0 cell growth is slower than wild type in glycerol and ethanol medium ------- COMMENT: e69ef00f0c3516d9 8 nQPgicxEO+adefY/u1Jw2F2pUhY Coq4 protein is decreased but Dlp1, Coq3, Coq5 and Coq8 are not ------- COMMENT: e69ef00f0c3516d9 9 HWw4NZKGRP7JRmnWMA5ADluZN1s Coq4 protein is increased but Dlp1, Coq3, Coq5, and Coq8 are not ------- COMMENT: e69ef00f0c3516d9 10 3r2HvUlAKo0GSOCs3Kprp02n0bw cell growth is faster than wild type in glycerol and ethanol medium ------- COMMENT: e69ef00f0c3516d9 11 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 12 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 13 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 14 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 15 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 16 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 17 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 18 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 19 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 20 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 21 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 22 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 23 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 24 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 25 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 26 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 27 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 28 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 29 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 30 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 31 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 32 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 33 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 34 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 35 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 36 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 37 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 38 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 39 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 40 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 41 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 42 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 43 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 44 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: e69ef00f0c3516d9 48 3V3HaMQsZES9F/qR1JrWhOrHbFU Fig6 ------- COMMENT: e69ef00f0c3516d9 49 1HG7HkAze7m1hZX5NVXtHu9jutE Fig7 ------- COMMENT: e69ef00f0c3516d9 50 1HG7HkAze7m1hZX5NVXtHu9jutE Fig7 ------- COMMENT: e69ef00f0c3516d9 51 1HG7HkAze7m1hZX5NVXtHu9jutE Fig7 ------- COMMENT: e69ef00f0c3516d9 52 NX6v/toclXh9222PH8RUlVRwEAI not shown ------- COMMENT: e69ef00f0c3516d9 53 uP2b450kx3y/u6z3D7bDUa+eEuM fig8 ------- COMMENT: e6d012dea46b05f6 13 94gANwyIY587AkzJ5+ufNB20yZU (comment: Ile AAU, Leu UAG, Leu CAG, Phe GAA, Ser GCU) ------- COMMENT: e6d012dea46b05f6 14 ZiH93jld2bCXzXlfFlGYD/pVBKc (comment: Asn GUU, Gly CCC, Ile AAU, Leu AAG, Leu CAA, Leu CAG, Leu UAG, Phe GAA, Ser AGA, Ser GCU, Thr AGU, Trp CAA) ------- COMMENT: e6d012dea46b05f6 15 bI6ac5nbr74Pb0nRfk6C7bObUBM affects tRNA-Ser UGA/CGA; suggests tRNA-Ser UGA/CGA misfolding due to decreased dimethylation of G26, but modification not assayed directly for this tRNA ------- COMMENT: e6d012dea46b05f6 16 bI6ac5nbr74Pb0nRfk6C7bObUBM affects tRNA-Ser UGA/CGA; suggests tRNA-Ser UGA/CGA misfolding due to decreased dimethylation of G26, but modification not assayed directly for this tRNA ------- COMMENT: e6d012dea46b05f6 17 FqHQXsojRx/jN9NJWlEAH3tBS8Q (comment: tRNA-Ser GCU and tRNA-Ser AGA unaffected) ------- COMMENT: e6d26db4caf74ac5 1 oXyxwMWUof8dtYaUN4Rl3+a1bXg Fig 1A rad1 is required for meiotic DNA replication checkpoint ------- COMMENT: e6d26db4caf74ac5 2 zSXJbmaJ9IwYllZ2jFx0m5MBlSk Fig1B cds1 is required for meiotic DNA replication checkpoint ------- COMMENT: e6d26db4caf74ac5 3 3EfvjaMCp77T+8C8sptTG84+fIc Fig1B ------- COMMENT: e6d26db4caf74ac5 4 3EfvjaMCp77T+8C8sptTG84+fIc Fig1B ------- COMMENT: e6d26db4caf74ac5 5 Weem98vuQIQTfgEPPr2cZ11MWS0 Fig 1B (comment: CHECK double cds1delta chk1 delta has same phenotype as single cds1delta/cds1 delta) ------- COMMENT: e6d26db4caf74ac5 7 E7q2HZYa9T2mniYQcPkJFfYj3xs Fig 2A, 2B ------- COMMENT: e6d26db4caf74ac5 8 E7q2HZYa9T2mniYQcPkJFfYj3xs Fig 2A, 2B ------- COMMENT: e6d26db4caf74ac5 10 orxQYIWqKHpBdRQj78f11rPWfBU Data not shown. kinetics same as pat1ts rad1delta diploid ------- COMMENT: e6d26db4caf74ac5 11 orxQYIWqKHpBdRQj78f11rPWfBU Data not shown. kinetics same as pat1ts rad1delta diploid ------- COMMENT: e6d26db4caf74ac5 12 orxQYIWqKHpBdRQj78f11rPWfBU Data not shown. kinetics same as pat1ts rad1delta diploid ------- COMMENT: e6d26db4caf74ac5 13 Qb8DOPQWL9mM6jy2IA7XCRXpAOg Data not shown prophase arrest with horsetail nuclear morphology see fig3A for pat1ts control ------- COMMENT: e6d26db4caf74ac5 14 Qb8DOPQWL9mM6jy2IA7XCRXpAOg Data not shown prophase arrest with horsetail nuclear morphology see fig3A for pat1ts control ------- COMMENT: e6d26db4caf74ac5 16 ZCVWSQ/j07cf98uM9w6SbVdZYRk Fig3B data not shown ------- COMMENT: e6d26db4caf74ac5 17 ZCVWSQ/j07cf98uM9w6SbVdZYRk Fig3B data not shown ------- COMMENT: e6d26db4caf74ac5 19 0hMT/FlqYC32qQmeF/kHCFxPsoA Fig3B ------- COMMENT: e6d26db4caf74ac5 20 0hMT/FlqYC32qQmeF/kHCFxPsoA Fig3B ------- COMMENT: e6d26db4caf74ac5 21 psJbSkNdsDHlhumDf18+Zf6KWvI Fig3B ------- COMMENT: e6d26db4caf74ac5 25 RxGFz0oy+Upt0jyk+ppUYcQXJCk Fig 4 ------- COMMENT: e6d26db4caf74ac5 26 v1uP5y4Q6x50PT5VNZuz0Pww2a4 Fig4 (comment: CHECK present during meiotic DNA replication checkpoint arrest) ------- COMMENT: e6d26db4caf74ac5 27 jyAMS+2VKOKw1ihqXh3TQXT1Chs Fig5A (comment: see control in Fig4A) ------- COMMENT: e6d26db4caf74ac5 31 O+goSz6zY+0pHaxT2c+UsaMZrU0 Fig5B (comment: see Fig4B for control) ------- COMMENT: e6d26db4caf74ac5 32 jyAMS+2VKOKw1ihqXh3TQXT1Chs Fig5A (comment: see control in Fig4A) ------- COMMENT: e6d26db4caf74ac5 33 /QAQ5GdJWLX7sU43WcL8guLlAqM Fig 5A (comment: see Fig4A for control) ------- COMMENT: e6d26db4caf74ac5 34 /QAQ5GdJWLX7sU43WcL8guLlAqM Fig 5A (comment: see Fig4A for control) ------- COMMENT: e6d26db4caf74ac5 35 OXqCw37/Uyk9HcquOWmbm+UAQCU Fig6C ------- COMMENT: e6d26db4caf74ac5 37 AM3X+VWEg0xFiDxUApuw/KNTrZg Data not shown when rad1 is deleted checkpoint is not activated and cells attempt meiotic nuclear divisions see also Fig1, 2, 3B ------- COMMENT: e6d26db4caf74ac5 38 vhYG0vJpeWeGkoDJM/efZppj7Gc Fig6A, 6D; meiotic cells unable to inhibit CDK1 activity in response to activation of the meiotic DNA replication checkpoint, arrest at metaphase of Meiosis I and do not undergo nuclear division ------- COMMENT: e70a1374f7a8719d 1 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: e70a1374f7a8719d 2 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: e70a1374f7a8719d 5 c+qeJtxrtyPgru7Q6m94TFlfhKM fig 4b ------- COMMENT: e70a1374f7a8719d 6 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: e70a1374f7a8719d 10 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: e70a1374f7a8719d 11 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: e70a1374f7a8719d 12 NhnU/WNmZewNqhLVsZF38VT2sgM figure 5 ------- COMMENT: e70a1374f7a8719d 13 9FBpgUoJW1+Hp5dX6qX92XJ98J0 figure 6 ------- COMMENT: e70a1374f7a8719d 14 srNPEOHUR5maeOFJvV9hJaeBsr4 fig 6c ------- COMMENT: e70a1374f7a8719d 16 UYAiwu33UOwjTIyR+DhHQ8gGrkk fig 6a ------- COMMENT: e70a1374f7a8719d 17 UYAiwu33UOwjTIyR+DhHQ8gGrkk fig 6a ------- COMMENT: e70a1374f7a8719d 18 UYAiwu33UOwjTIyR+DhHQ8gGrkk fig 6a ------- COMMENT: e70a1374f7a8719d 19 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: e70a1374f7a8719d 20 W6s5iQeJIKaeaB30bogGN7/fynU fig 7 ------- COMMENT: e720f14179755b56 1 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: e720f14179755b56 2 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: e720f14179755b56 3 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: e720f14179755b56 4 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: e720f14179755b56 5 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: e720f14179755b56 7 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: e720f14179755b56 8 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: e720f14179755b56 9 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: e720f14179755b56 10 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: e720f14179755b56 11 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: e720f14179755b56 12 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: e720f14179755b56 13 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: e720f14179755b56 14 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: e720f14179755b56 15 MpML2E+8wq5nwC1YfM2LDELzzPU Fig. 2E ------- COMMENT: e720f14179755b56 16 MpML2E+8wq5nwC1YfM2LDELzzPU Fig. 2E ------- COMMENT: e720f14179755b56 17 MpML2E+8wq5nwC1YfM2LDELzzPU Fig. 2E ------- COMMENT: e720f14179755b56 18 MpML2E+8wq5nwC1YfM2LDELzzPU Fig. 2E ------- COMMENT: e73b6293989d19a9 1 laWIOFkC+GHQyn77SnMIl9+Keas (comment: CHECK activated_by(CHEBI:29108)| activated_by(CHEBI:29035)) ------- COMMENT: e73fcd0460d8dc7f 1 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: e73fcd0460d8dc7f 2 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: e73fcd0460d8dc7f 3 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: e73fcd0460d8dc7f 4 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: e73fcd0460d8dc7f 5 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: e73fcd0460d8dc7f 6 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: e73fcd0460d8dc7f 7 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: e73fcd0460d8dc7f 8 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: e73fcd0460d8dc7f 9 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: e73fcd0460d8dc7f 10 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: e73fcd0460d8dc7f 11 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: e73fcd0460d8dc7f 12 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: e73fcd0460d8dc7f 14 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: e73fcd0460d8dc7f 15 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: e73fcd0460d8dc7f 16 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: e73fcd0460d8dc7f 17 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: e73fcd0460d8dc7f 18 p23VaE2MmzhdxFHAPnFMA17hdOk Fig. 2A, 2B ------- COMMENT: e73fcd0460d8dc7f 19 p23VaE2MmzhdxFHAPnFMA17hdOk Fig. 2A, 2B ------- COMMENT: e73fcd0460d8dc7f 20 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: e73fcd0460d8dc7f 21 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: e73fcd0460d8dc7f 22 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: e73fcd0460d8dc7f 23 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: e73fcd0460d8dc7f 24 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: e73fcd0460d8dc7f 25 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: e73fcd0460d8dc7f 26 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: e73fcd0460d8dc7f 27 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: e73fcd0460d8dc7f 28 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: e73fcd0460d8dc7f 29 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: e73fcd0460d8dc7f 30 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: e73fcd0460d8dc7f 31 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: e73fcd0460d8dc7f 32 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: e73fcd0460d8dc7f 33 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: e73fcd0460d8dc7f 34 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: e73fcd0460d8dc7f 35 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: e73fcd0460d8dc7f 36 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: e73fcd0460d8dc7f 37 9ajXo/NIe7Cu/Mz5lbMamOAvYjs Fig. 7A ------- COMMENT: e73fcd0460d8dc7f 38 JUKiF37j64aMaKpSH0OBgfVgnXQ Fig. 8A ------- COMMENT: e73fcd0460d8dc7f 39 J/L/vbnO0f4RKH9ckj3qYC1p0fc Fig. 8B ------- COMMENT: e740e740b75c99d4 15 CFbvOC4LkoqbdLfBdgrf8jzpVYo growing tips were longer and thinner than those of wild-type cells. This morphology is similar to the one caused by overexpression of Rga4. ------- COMMENT: e740e740b75c99d4 16 s5/FRC5LNDl7HbIDu8Uhy5JSS3g (comment: RhoGAP, GTPase activating protein for Cdc42 and Rho2) ------- COMMENT: e740e740b75c99d4 43 Oifi7LzNIE+jOg6idSMda0+ui24 (comment: hard to be more specific when cell shape is also abnormal (Rga6 normally goes to lateral cortex & non-growing tip)) ------- COMMENT: e740e740b75c99d4 44 Pf5ihccTlmAiBcTYs6Ln7zUDpe4 (comment: can't assess viability) ------- COMMENT: e740e740b75c99d4 45 Pf5ihccTlmAiBcTYs6Ln7zUDpe4 (comment: can't assess viability) ------- COMMENT: e740e740b75c99d4 46 Pf5ihccTlmAiBcTYs6Ln7zUDpe4 (comment: can't assess viability) ------- COMMENT: e740e740b75c99d4 47 Pf5ihccTlmAiBcTYs6Ln7zUDpe4 (comment: can't assess viability) ------- COMMENT: e740e740b75c99d4 48 Pf5ihccTlmAiBcTYs6Ln7zUDpe4 (comment: can't assess viability) ------- COMMENT: e740e740b75c99d4 49 Pf5ihccTlmAiBcTYs6Ln7zUDpe4 (comment: can't assess viability) ------- COMMENT: e740e740b75c99d4 50 Pf5ihccTlmAiBcTYs6Ln7zUDpe4 (comment: can't assess viability) ------- COMMENT: e7582f1802a42934 28 P/8qpetu6E9eYaIDTgr+3y8syL0 Fig. S2A ------- COMMENT: e7582f1802a42934 29 P/8qpetu6E9eYaIDTgr+3y8syL0 Fig. S2A ------- COMMENT: e7582f1802a42934 30 P/8qpetu6E9eYaIDTgr+3y8syL0 Fig. S2A ------- COMMENT: e75b1422bd29e157 6 O5LQLQ9NSlrv31e1n/ORHh/7nGw (comment: movement in anap[hase A) ------- COMMENT: e75b1422bd29e157 15 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: e75b1422bd29e157 16 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: e75b1422bd29e157 17 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: e75b1422bd29e157 18 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: e75b1422bd29e157 19 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: e75b1422bd29e157 20 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: e79441aacbe75066 4 J/9r++WuayHqJRn5V2wj7JGoiL8 in supp fig1 shows weak sensitivity at high cadmium concentrations ------- COMMENT: e7cc68a1da978b38 1 IW0Z7pW4r1eGq7g+qTrUfAXH4nI Fig1B cells blocked in G1 by nitrogen starvation and released in presence of nitrogen into S phase with cdc13+ switched off ------- COMMENT: e7cc68a1da978b38 2 bdrGW7nyf608ct3DD7OoWBl0NPU FigS1A, S1C cells arrested G1 in low nitrogen then released into S phase at restrictive temperature cells the tested for viability at 25°C after S phase with only ccdc13hpm ------- COMMENT: e7cc68a1da978b38 3 r8yI6nyoQJb4t5bqF51i8Zd1+Ts Fig S1D cdc13+ and cdc13HPM are not differentially sensitive to rum1. S phase same in both strains in absence of rum1 ------- COMMENT: e7cc68a1da978b38 4 tIb+Of8PIHKsI1mFMhvNNpQ1y4k FigS1-E cdc2 Y15 phosphorylation same in cdc13+ control and cdc13HPM strain endogenous cdc13+ is completely degraded so does not contribute in the HPM mutant ------- COMMENT: e7cc68a1da978b38 6 /shKXXUVwPdvHJl989R0eD9Dwtw Fig2B-D Endogenous untagged nmt 41cdc13+ is expressed to allow cells to proceed into mitosis tagged exogenous cdc13HPM or cdc13+ control can be seen at SPB ------- COMMENT: e7cc68a1da978b38 7 fI9RUyLuaIqXoINs65WsyEQ31gw Fig2A cells are unable to enter mitosis in absence of cdc13+ expression-no septated cells ------- COMMENT: e7cc68a1da978b38 8 knJMvgIYp7zolZqoJdyiwtdBGHk Fig2A cells expressing only cdc13HPM are unable to form colonies ------- COMMENT: e7cc68a1da978b38 9 yCHPxdxH5flN0rtQwgminoHtNbQ cdc13HPM mutant can localise to SPB in mitosis ------- COMMENT: e7cc68a1da978b38 12 CEP8NJyk9yHpwkUc0gvWlLict5s Fig 2G when plo1 is advanced on to the spindle pole body cdc13HPM is also advanced ------- COMMENT: e7cc68a1da978b38 13 tgTgvLFeiZFj1npdKNU3j3fftKk Fig 2H when plo1 kinase is inactivated at the restrictive temperature the HPM mutant does not bind to the SPB after release into mitosis ------- COMMENT: e7cc68a1da978b38 15 fEVdJnFlZ42YNmodTp3kEv6qnxc Fig2H cdc13HPM localisation to SPB in mitosis is dependent on plo1 activity ------- COMMENT: e7cc68a1da978b38 16 znCzJFs6+q0ug3QSjXm01QdCz7s Fig3A when an integrated copy of cdc13HPM (at leu1 locus) is expressed from the cdc13 promoter the endogenous cdc13+ cells are advanced into mitosis. This suggests cdc13HPM can do some of events required for mitotic entry. This is independent of the G1/S cyclins ------- COMMENT: e7cc68a1da978b38 18 XqdYTXwEZU2jpKGENhMAjh7dMEA cdc13HPM mutant fails to localise to the SPB during G2 ------- COMMENT: e7cc68a1da978b38 21 CEP8NJyk9yHpwkUc0gvWlLict5s Fig 2G when plo1 is advanced on to the spindle pole body cdc13HPM is also advanced ------- COMMENT: e7cc68a1da978b38 22 CEP8NJyk9yHpwkUc0gvWlLict5s Fig 2G when plo1 is advanced on to the spindle pole body cdc13HPM is also advanced ------- COMMENT: e7cc68a1da978b38 24 BTLhLt/kHBON3BxRP3zi2YBPAH8 FIGURE S1E Wee1-dependent CDK-Y15 phosphorylation was similar between Cdc13HPM-CDK and Cdc13WT-CDK ------- COMMENT: e7cc68a1da978b38 25 fEVdJnFlZ42YNmodTp3kEv6qnxc Fig2H cdc13HPM localisation to SPB in mitosis is dependent on plo1 activity ------- COMMENT: e7e1ea48e4e672fc 1 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: e7e1ea48e4e672fc 2 dv0FwaxoIZZGekUorXD8zABlJNU fig 1c ------- COMMENT: e7e1ea48e4e672fc 3 Y4oOTioFkAAoVUId7q6lTRyGWkI Fig1D (comment: actually 2 bundles) ------- COMMENT: e7e1ea48e4e672fc 4 S+kgrJ2UJ65gKPwQrdvSd5wE4jM (comment: from both ends) ------- COMMENT: e7e1ea48e4e672fc 5 S+kgrJ2UJ65gKPwQrdvSd5wE4jM (comment: from both ends) ------- COMMENT: e7e1ea48e4e672fc 6 z1is36PhN5+M+0MktOY90YfgJcE Figure 5B ------- COMMENT: e7e1ea48e4e672fc 8 cBSApSDxmqXRugy/9A5lbn1RBr4 (comment: emtoc) ------- COMMENT: e80e3736b70fa4b5 1 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: e80e3736b70fa4b5 3 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: e80e3736b70fa4b5 4 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: e80e3736b70fa4b5 5 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: e80e3736b70fa4b5 6 3f/pvMLKY9m3+KB4lHfLMSa9SG0 figure 1d ------- COMMENT: e80e3736b70fa4b5 7 XLIT4KG+CcooyfKJG7ccSGSiDzE figure 1c, 1d ------- COMMENT: e80e3736b70fa4b5 8 j0sFwPNhhHJ/lr9E3wNigYLUxSM figure 1D ------- COMMENT: e80e3736b70fa4b5 9 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: e80e3736b70fa4b5 10 umWW1WAjGP/yg9XcrngizGWIbDA Figure 2. ------- COMMENT: e80e3736b70fa4b5 11 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: e80e3736b70fa4b5 12 1uEOcFf8lGNAEmweMU80cSD2nEU Figure 3. ------- COMMENT: e80e3736b70fa4b5 13 1uEOcFf8lGNAEmweMU80cSD2nEU Figure 3. ------- COMMENT: e80e3736b70fa4b5 14 1uEOcFf8lGNAEmweMU80cSD2nEU Figure 3. ------- COMMENT: e80e3736b70fa4b5 15 1uEOcFf8lGNAEmweMU80cSD2nEU Figure 3. ------- COMMENT: e80e3736b70fa4b5 18 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: e80e3736b70fa4b5 19 iAcbpl1ew+nFwb8AiAqOLZxpvN8 Figure 1D ------- COMMENT: e80e3736b70fa4b5 20 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: e80e3736b70fa4b5 21 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: e80e3736b70fa4b5 22 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: e80e3736b70fa4b5 23 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: e80e3736b70fa4b5 25 1uEOcFf8lGNAEmweMU80cSD2nEU Figure 3. ------- COMMENT: e80e3736b70fa4b5 26 1uEOcFf8lGNAEmweMU80cSD2nEU Figure 3. ------- COMMENT: e80e3736b70fa4b5 27 1uEOcFf8lGNAEmweMU80cSD2nEU Figure 3. ------- COMMENT: e80e3736b70fa4b5 28 1uEOcFf8lGNAEmweMU80cSD2nEU Figure 3. ------- COMMENT: e80e3736b70fa4b5 29 CHpJ2wHUt5js5SYW+L/BTDKlRvw figure 1b divides longer than WT in the same conditions ------- COMMENT: e81392bd8d0bb821 23 hARLHXsSx2nKoEeXX1uJPlMD/HU (comment: three-hybrid assay involving Uaf2, Prp2, and an RNA fragment containing the heterologous beta-globin 3′ splice site) ------- COMMENT: e81eaddcfa17ed1c 6 7REl1FKjDA7OYlhgU+9qn7iaGp8 (comment: haploid, either mating type) ------- COMMENT: e81eaddcfa17ed1c 11 kccmSWlFoLWXUTSPwKVthsnf2vc (comment: changed to GTPase from signal transducer) ------- COMMENT: e83166112a9102b6 1 GZ99OjrRJLimMNpcDTRpuud2ksM (comment: CHECK inhibited_by CHEBI:27266) ------- COMMENT: e865b65eeb6f06b0 2 Ci+bKhUitsutwHeHgf5TiETS96Y Fig1 ------- COMMENT: e865b65eeb6f06b0 4 Ci+bKhUitsutwHeHgf5TiETS96Y Fig1 ------- COMMENT: e865b65eeb6f06b0 5 msbvsxkadTEsKxypvLfAhTxd78s Fig2 ------- COMMENT: e865b65eeb6f06b0 6 msbvsxkadTEsKxypvLfAhTxd78s Fig2 ------- COMMENT: e865b65eeb6f06b0 7 msbvsxkadTEsKxypvLfAhTxd78s Fig2 ------- COMMENT: e865b65eeb6f06b0 8 msbvsxkadTEsKxypvLfAhTxd78s Fig2 ------- COMMENT: e865b65eeb6f06b0 9 msbvsxkadTEsKxypvLfAhTxd78s Fig2 ------- COMMENT: e865b65eeb6f06b0 11 Ag6GoP3zsrPsg4IZ5Mmwasgbws4 Fig3 ------- COMMENT: e865b65eeb6f06b0 12 Ag6GoP3zsrPsg4IZ5Mmwasgbws4 Fig3 ------- COMMENT: e865b65eeb6f06b0 13 Ag6GoP3zsrPsg4IZ5Mmwasgbws4 Fig3 ------- COMMENT: e865b65eeb6f06b0 14 Ag6GoP3zsrPsg4IZ5Mmwasgbws4 Fig3 ------- COMMENT: e865b65eeb6f06b0 18 LEREx9B0cGjhIuNqZtZ6xLIkciQ and observed a preferential association of Swi6 with Y41F over Y41p peptide, suggest- ing that phosphorylation of H3Y41 counteracts the interac- tion of Swi6 with histone H3 (Supplementary Figure S6A). ------- COMMENT: e865b65eeb6f06b0 19 LEREx9B0cGjhIuNqZtZ6xLIkciQ and observed a preferential association of Swi6 with Y41F over Y41p peptide, suggest- ing that phosphorylation of H3Y41 counteracts the interac- tion of Swi6 with histone H3 (Supplementary Figure S6A). ------- COMMENT: e865b65eeb6f06b0 20 LEREx9B0cGjhIuNqZtZ6xLIkciQ and observed a preferential association of Swi6 with Y41F over Y41p peptide, suggest- ing that phosphorylation of H3Y41 counteracts the interac- tion of Swi6 with histone H3 (Supplementary Figure S6A). ------- COMMENT: e865b65eeb6f06b0 21 h09KQpN7u/q6m3yDchMr2e10lc8 Fig4 ------- COMMENT: e865b65eeb6f06b0 22 h09KQpN7u/q6m3yDchMr2e10lc8 Fig4 ------- COMMENT: e865b65eeb6f06b0 23 5V4z8e1gNp3HPryJ8ie/6SwTjpg Fig4, 5 ------- COMMENT: e865b65eeb6f06b0 24 5V4z8e1gNp3HPryJ8ie/6SwTjpg Fig4, 5 ------- COMMENT: e865b65eeb6f06b0 28 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: e8a53aa5aa4d49f7 3 r+bCKkbPMMvqk2R/1BUNZOb+PeU the CAF-1 complex promotes Replication-coupled homologous recombination at blocked replication forks. ------- COMMENT: e8a53aa5aa4d49f7 4 r+bCKkbPMMvqk2R/1BUNZOb+PeU the CAF-1 complex promotes Replication-coupled homologous recombination at blocked replication forks. ------- COMMENT: e8a53aa5aa4d49f7 5 r+bCKkbPMMvqk2R/1BUNZOb+PeU the CAF-1 complex promotes Replication-coupled homologous recombination at blocked replication forks. ------- COMMENT: e8ac2521a0651d1d 9 c5LBm4Gcnb7w51YLjpGAaUuuQ2A (comment: CHECK same as cdc18+ oe alone) ------- COMMENT: e8ac2521a0651d1d 10 c5LBm4Gcnb7w51YLjpGAaUuuQ2A (comment: CHECK same as cdc18+ oe alone) ------- COMMENT: e8bc7aa4408219ce 1 KCTtreA1lto08vL9ZstxrUqcLck (Fig. 1) 77% asymmetric distribution by 5 hours, aggregation observed after 1 hour ------- COMMENT: e8bc7aa4408219ce 2 Nj6OF05m70IDQp4GzpuBZHBl6tY 73% asymmetric distribution at old end by 5 hours asymmetric mitochondrial aggregation at old cell end ------- COMMENT: e8bc7aa4408219ce 3 UqFJl07W8EIqeF8s61u9eKmFLuo (comment: data not shown) ------- COMMENT: e8bc7aa4408219ce 4 OSGz5nledahmWHgSVVPqcnYpQAM data not shown, cells blocked at G1/S, cells need to complete cell cycle to observe asymmetry ------- COMMENT: e8bc7aa4408219ce 5 ywHShRvn+L6KaH29Zt3nsq+qYC8 data not shown, cells blocked in S phase, cells need to complete cell cycle to observe asymmetry ------- COMMENT: e8bc7aa4408219ce 6 IGlShg9M/XYuvI7avJ66e1iqdrQ data not shown, cells blocked at G2/M, cells need to complete cell cycle to observe asymmetry ------- COMMENT: e8bc7aa4408219ce 7 gCd5BmIeX8BdRswQLA05e1kTU5o data not shown, cells blocked in absence of septation, cells need to complete cell cycle to observe asymmetry ------- COMMENT: e8bc7aa4408219ce 8 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: e8bc7aa4408219ce 9 x+x7cO6VWNmZb5ds1icfOMEyJlY (Fig. 2) 1% of cells still have a short mitotic spindle after 5h at restrictive temperature ------- COMMENT: e8bc7aa4408219ce 10 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: e8bc7aa4408219ce 11 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: e8bc7aa4408219ce 12 h09KQpN7u/q6m3yDchMr2e10lc8 Fig4 ------- COMMENT: e8bfc899ec0c73d9 16 1bEOJ/uo4tUIV5/cK8I36avE7WI (comment: CHECK same as either single mutant) ------- COMMENT: e8bfc899ec0c73d9 30 V8fcFA8KwouLR6JuTO0WyLYM32A (comment: CHECK same as exo1delta alone) ------- COMMENT: e8bfc899ec0c73d9 38 fgtW+4SZRPx6dcylna3rmbCGIfE (comment: upstream reporter) ------- COMMENT: e8bfc899ec0c73d9 39 fgtW+4SZRPx6dcylna3rmbCGIfE (comment: upstream reporter) ------- COMMENT: e8e4518f9c7db116 1 rm0I4ZDDwbAD9L6J1c/ddi/hVvc severe growth delay on both fermentable (Glucose) and respiratory (Ethanol Glycerol) media at the restrictive temperature, while it behaved like the wild-type at permissive tempera- ture ------- COMMENT: e8e4518f9c7db116 2 4CWY+qMt2R2Os+8D3Ahlz64T6xc Fig. 1b severe growth delay on both fermentable (Glucose) and respiratory (Ethanol Glycerol) media at the restrictive temperature, while it behaved like the wild-type at permissive tempera- ture ------- COMMENT: e8e4518f9c7db116 3 rm0I4ZDDwbAD9L6J1c/ddi/hVvc severe growth delay on both fermentable (Glucose) and respiratory (Ethanol Glycerol) media at the restrictive temperature, while it behaved like the wild-type at permissive tempera- ture ------- COMMENT: e8e4518f9c7db116 4 rm0I4ZDDwbAD9L6J1c/ddi/hVvc severe growth delay on both fermentable (Glucose) and respiratory (Ethanol Glycerol) media at the restrictive temperature, while it behaved like the wild-type at permissive tempera- ture ------- COMMENT: e8e4518f9c7db116 5 rm0I4ZDDwbAD9L6J1c/ddi/hVvc severe growth delay on both fermentable (Glucose) and respiratory (Ethanol Glycerol) media at the restrictive temperature, while it behaved like the wild-type at permissive tempera- ture ------- COMMENT: e8e4518f9c7db116 6 rm0I4ZDDwbAD9L6J1c/ddi/hVvc severe growth delay on both fermentable (Glucose) and respiratory (Ethanol Glycerol) media at the restrictive temperature, while it behaved like the wild-type at permissive tempera- ture ------- COMMENT: e8e4518f9c7db116 7 rm0I4ZDDwbAD9L6J1c/ddi/hVvc severe growth delay on both fermentable (Glucose) and respiratory (Ethanol Glycerol) media at the restrictive temperature, while it behaved like the wild-type at permissive tempera- ture ------- COMMENT: e8e4518f9c7db116 8 rm0I4ZDDwbAD9L6J1c/ddi/hVvc severe growth delay on both fermentable (Glucose) and respiratory (Ethanol Glycerol) media at the restrictive temperature, while it behaved like the wild-type at permissive tempera- ture ------- COMMENT: e8e4518f9c7db116 9 +r4pyuTBerkXRnSJTgxFrBqERTM Fig. 1c ------- COMMENT: e8e4518f9c7db116 11 2kuSN7rMzfGcB2DKt67EqDWQELA Fig. 2 ------- COMMENT: e8e4518f9c7db116 12 2kuSN7rMzfGcB2DKt67EqDWQELA Fig. 2 ------- COMMENT: e8e4518f9c7db116 13 d95o2uzYI7q7tY7bHI4U1xBug7s Fig. 3 ------- COMMENT: e8e4518f9c7db116 14 d95o2uzYI7q7tY7bHI4U1xBug7s Fig. 3 ------- COMMENT: e8e4518f9c7db116 15 d95o2uzYI7q7tY7bHI4U1xBug7s Fig. 3 ------- COMMENT: e8e4518f9c7db116 16 Ny6gjKsz5xwCxlHbyDpHTTLGduo (comment: text) ------- COMMENT: e8e4518f9c7db116 17 Ny6gjKsz5xwCxlHbyDpHTTLGduo (comment: text) ------- COMMENT: e8e4518f9c7db116 18 Ny6gjKsz5xwCxlHbyDpHTTLGduo (comment: text) ------- COMMENT: e905a9d566f724ce 1 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: e905a9d566f724ce 2 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: e905a9d566f724ce 3 vfbMK2Rl/YuyqfnruckDDYPnqHM Figure 1 (comment: CHECK in interphase) ------- COMMENT: e905a9d566f724ce 4 vfbMK2Rl/YuyqfnruckDDYPnqHM Figure 1 (comment: CHECK in interphase) ------- COMMENT: e92c2476d90dea83 11 rEMPLgnSClXOzdtYaA1zHpgpizc (comment: CHECK same as cps8-185 alone) ------- COMMENT: e92fbfc95586af82 5 rxw0vmMhHrQR9gopCxGVHUagCgg (comment: CHECK this is a protein modification so should be changed once we can do RNA mods) ------- COMMENT: e930f865a30e2c82 38 jlLvUCC23POs4ZFtGDUVNdIMvwk (comment: This comes from point mutation in FLEX moitf abolished transcription. Phenotype is not captured because the precise mutant could not be established.) ------- COMMENT: e9324976499786d4 2 cqmyOQiNhSfP1GFNCNYbrt6NY7M (comment: catechol O-methyltransferase activity (Vw I kept this as o-methytransferase since no report of catachols in fission yeast)) ------- COMMENT: e9324976499786d4 3 l7PfLRmm6C4NHTYS5R7kDdsgLuE (comment: catechol O-methyltransferase activity) ------- COMMENT: e9324976499786d4 4 /3TQNr0JDgeB9lpu5Tf1WEWNXvs (comment: detoxification) ------- COMMENT: e9324976499786d4 5 /3TQNr0JDgeB9lpu5Tf1WEWNXvs (comment: detoxification) ------- COMMENT: e9611fbd9aa9473a 11 NKbVEw5untp77BVAqkTHmm4bESI (comment: CHECK low expressivity) ------- COMMENT: e9611fbd9aa9473a 13 TZ8PsNy/WqX+BVXcV790pwyphns (comment: CHECK high expressivity) ------- COMMENT: e96bae1f0363904c 9 kA92FLwfG/tu5yyKwB2NHH8HwjY assayed in S. cerevisiae cell extracts, with S.c. CDK2 substrate ------- COMMENT: e96bae1f0363904c 10 G3IQkS5HNPkZgbR/+ZJFXWUFPKM assayed in S. cerevisiae cell extracts, with S.c. CTD substrate ------- COMMENT: e9763156a337dd76 1 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: e9763156a337dd76 2 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: e9763156a337dd76 3 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: e9763156a337dd76 4 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: e9763156a337dd76 5 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: e9763156a337dd76 6 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: e9763156a337dd76 7 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: e9763156a337dd76 8 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: e9763156a337dd76 9 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: e9763156a337dd76 10 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: e9763156a337dd76 11 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: e9763156a337dd76 12 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: e9b6581da99a35cf 7 7tMaWl4dHZPsmI2c6BZ8aZyIrsc (comment: CHECK 25S rRNA positions 2304, 2497) ------- COMMENT: e9c626e3d4661f4d 1 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: e9c626e3d4661f4d 2 HJfU8mLX+ptAhOhj6X++o3ZtSQc The deletion of epe1+, which encodes a putative histone H3K9 demethylase, did not affect the establishment of silencing. Fig. 2 ------- COMMENT: e9c626e3d4661f4d 3 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: e9c626e3d4661f4d 4 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: e9c626e3d4661f4d 5 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: e9c626e3d4661f4d 6 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: e9c626e3d4661f4d 7 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: e9c626e3d4661f4d 8 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: e9c626e3d4661f4d 9 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: e9c626e3d4661f4d 10 wr5TXzzUQ5a8pjtOfW1cRWvVFaY Fig. 7D ------- COMMENT: e9c626e3d4661f4d 11 wr5TXzzUQ5a8pjtOfW1cRWvVFaY Fig. 7D ------- COMMENT: e9c626e3d4661f4d 12 wr5TXzzUQ5a8pjtOfW1cRWvVFaY Fig. 7D ------- COMMENT: e9c626e3d4661f4d 13 ecwX4alqEfu2i+w/vkmTFW1tmRs Fig. 7E ------- COMMENT: e9c626e3d4661f4d 14 ecwX4alqEfu2i+w/vkmTFW1tmRs Fig. 7E ------- COMMENT: e9c626e3d4661f4d 15 ecwX4alqEfu2i+w/vkmTFW1tmRs Fig. 7E ------- COMMENT: e9e39a22b3cf3516 2 brrAwOXKFruRtc9knlEMRwE9UjY (comment: there is another unknown gene with this activity) ------- COMMENT: e9e39a22b3cf3516 4 IrEWJItlbyGLzqwIgoAUnLEhA3A (comment: decreased) ------- COMMENT: e9f007db7b387da3 1 U1NiAwkwFveDm8425RQEx2O6HgY Fig5 ------- COMMENT: e9f007db7b387da3 2 3EfvjaMCp77T+8C8sptTG84+fIc Fig1B ------- COMMENT: e9f007db7b387da3 3 BYwbAFhRVbtdv430lGRLRz9UGxY Fig1C Peaks at the end of G2 40 min before peak of rum1 protein ------- COMMENT: e9f007db7b387da3 4 3EfvjaMCp77T+8C8sptTG84+fIc Fig1B ------- COMMENT: e9f007db7b387da3 5 msbvsxkadTEsKxypvLfAhTxd78s Fig2 ------- COMMENT: e9f007db7b387da3 6 msbvsxkadTEsKxypvLfAhTxd78s Fig2 ------- COMMENT: e9f007db7b387da3 8 rpd2in4LBuUBXAmgfXKx7+5j1Zg Fig3, data not shown phenotype similar to rum+OP ------- COMMENT: e9f007db7b387da3 9 /ikhbQ9vy+0G1oAYI+7A7ZMT4Mc Fig3B, similar to rum1+OP more severe than either single mutant. (comment: expressed from muliticopy plasmid. Colonies were integrants) ------- COMMENT: e9f007db7b387da3 10 SZu6vybOwwMmEZ65AsejJzD5hYc Fig3C (comment: integrated copy) ------- COMMENT: e9f007db7b387da3 11 Si/cNpRk4i3+L2XUC8hx6jptB14 Fig 4B (comment: inhibitory for cdc2/cdc13 and cdc2/cig2 but not cdc2/cig1. Both Rum1+ and Rum1-A58A62 can inhibit cdk1 activity) ------- COMMENT: e9f007db7b387da3 12 hKWCsgQ9BP4CeUjS4JB+UtD3J0c Fig3C ------- COMMENT: e9f007db7b387da3 14 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: e9f007db7b387da3 15 3V3HaMQsZES9F/qR1JrWhOrHbFU Fig6 ------- COMMENT: e9f007db7b387da3 16 vnLsz6TvZTTmPAXTi6y56Sl0CYo Fig7, Fig8 cdc2-cig1 complex efficiently phosphorylates rum1 T58T62 residues in vivo . Phosphorylation by cdc2-cig2 or cdc2-cdc13 only observed after a very long exposure ------- COMMENT: e9f007db7b387da3 17 lXykFaUv669xW0RbVsLyp4ZQwf8 Fig 10 ------- COMMENT: e9f007db7b387da3 18 a2TetNNNKAHSCCGQC/oMqCJvTJU Fig7B rum1 A58A62 mutant protein is unable to be phosphorylated by cdc2/cig1 ------- COMMENT: e9f007db7b387da3 19 3EfvjaMCp77T+8C8sptTG84+fIc Fig1B ------- COMMENT: e9f007db7b387da3 20 Si/cNpRk4i3+L2XUC8hx6jptB14 Fig 4B (comment: inhibitory for cdc2/cdc13 and cdc2/cig2 but not cdc2/cig1. Both Rum1+ and Rum1-A58A62 can inhibit cdk1 activity) ------- COMMENT: e9f007db7b387da3 26 eoCNDX/kIs+LWsVlrra7GnrJ0k4 (comment: this isn't quite the right way to capture this target, still thinking) ------- COMMENT: e9f007db7b387da3 27 eoCNDX/kIs+LWsVlrra7GnrJ0k4 (comment: this isn't quite the right way to capture this target, still thinking) ------- COMMENT: e9f007db7b387da3 28 vnLsz6TvZTTmPAXTi6y56Sl0CYo Fig7, Fig8 cdc2-cig1 complex efficiently phosphorylates rum1 T58T62 residues in vivo . Phosphorylation by cdc2-cig2 or cdc2-cdc13 only observed after a very long exposure ------- COMMENT: ea1aba50125a8d9b 1 vgWoY59hB7mlj3RhWSXj6hHjh80 (comment: I don't really know how to do this: I would like to say that SPAC824.04, Ppn1 and Dis2 are part of a protein module associated with the CPF. We have named this module the DPS module. Lack of this module does not affect the formation of the core CPF (all other CPF sub-units remain associated as a complex). ) ------- COMMENT: ea1aba50125a8d9b 2 vgWoY59hB7mlj3RhWSXj6hHjh80 (comment: I don't really know how to do this: I would like to say that SPAC824.04, Ppn1 and Dis2 are part of a protein module associated with the CPF. We have named this module the DPS module. Lack of this module does not affect the formation of the core CPF (all other CPF sub-units remain associated as a complex). ) ------- COMMENT: ea1aba50125a8d9b 3 vgWoY59hB7mlj3RhWSXj6hHjh80 (comment: I don't really know how to do this: I would like to say that SPAC824.04, Ppn1 and Dis2 are part of a protein module associated with the CPF. We have named this module the DPS module. Lack of this module does not affect the formation of the core CPF (all other CPF sub-units remain associated as a complex). ) ------- COMMENT: ea1ec138d2671e21 3 sgMmi3wK+dJBlkGL3QsG5hsV9c0 Fig. 1a ------- COMMENT: ea1ec138d2671e21 4 uwFh2QV/iKFbm+C5ayYF8p9FCq0 Fig. 1a–d ------- COMMENT: ea1ec138d2671e21 5 uwFh2QV/iKFbm+C5ayYF8p9FCq0 Fig. 1a–d ------- COMMENT: ea1ec138d2671e21 6 uwFh2QV/iKFbm+C5ayYF8p9FCq0 Fig. 1a–d ------- COMMENT: ea1ec138d2671e21 7 LEZz7/skiwGgie/8jlVhqNXZKCY (Supplementary Fig. 2a) We confirmed that Psc3–2CD, as well as 2CD, itself localizesat discrete nuclear dots in swi6D cell ------- COMMENT: ea1ec138d2671e21 8 1htmqGFUILiCusFYSMMZX3oY9G0 (Supplementary Fig. 2a) , but not in another heterochromatin-defect- ive strain, clr4D, which lacks H3K9me (ref. 7) ------- COMMENT: ea1ec138d2671e21 9 Sto14mIOEyGS2EZrYXqUq2gIO3o (Fig. 1e, 1f) As predicted, the additional expression of Psc3–2CD (but not of 2CD alone) improved the localization of the cohesin complex to the peri-centromeric regions and also centromeric cohesion in swi6D cells ------- COMMENT: ea1ec138d2671e21 10 NF29fS/c/agA46INLbujZukFBC4 (Supplementary Fig. 3a). We confirmed that the expression of Psc3–2CD does not restore transcriptional silencing in swi6D cells ------- COMMENT: ea1ec138d2671e21 12 dJ32a/cTMwreXa7qdWlCxMG8bro figure 1g (comment: 30% cells?) ------- COMMENT: ea1ec138d2671e21 14 aVBdXJoTkzfpTvjqIJCgpwqkx/k various: These results indicate that Swi6 is crucial in localizing Sgo1 and thereby promotes the protection of cohesin from separase during anaphase I. ------- COMMENT: ea1ec138d2671e21 17 apaLNc3Vx5TKKLd+6I1Y73uTnYo (Fig. 2a, 2b) As with sgo1D cells, swi6D cells undergo intact meiosis I but suffer a nondisjunction of sister chromatids in meiosis II ------- COMMENT: ea1ec138d2671e21 18 apaLNc3Vx5TKKLd+6I1Y73uTnYo (Fig. 2a, 2b) As with sgo1D cells, swi6D cells undergo intact meiosis I but suffer a nondisjunction of sister chromatids in meiosis II ------- COMMENT: ea1ec138d2671e21 19 hJPKZRj7jFsmRU83AOMhTRmJ9/0 (Supplementary Fig. 4). The transcriptional silencing of Swi6 is not relevant to this function, because swi6-sm1 cells have intact meiotic chromosome segregation ------- COMMENT: ea1ec138d2671e21 20 PKwrXr/tWJgTbD9P+seHpkgsJO4 (Fig. 2e) Sgo1 localization is impaired in swi6D cells ------- COMMENT: ea1ec138d2671e21 21 rqjU5rL14df9pYCrmx4zrBY1nno (Fig. 2f) Sgo1–CD did indeed localize at the centromere regardless of swi6D ------- COMMENT: ea1ec138d2671e21 22 apaLNc3Vx5TKKLd+6I1Y73uTnYo (Fig. 2a, 2b) As with sgo1D cells, swi6D cells undergo intact meiosis I but suffer a nondisjunction of sister chromatids in meiosis II ------- COMMENT: ea1ec138d2671e21 24 8jCPXzj5zy5fAmkgjOmVilFcgGo (Fig. 3c) Accordingly, the replacement of Val 242 with Glu (VE) in Sgo1 abolished the interaction with Swi6 while preserving the interaction with Par1, a subunit of PP2A. An immunoprecipitation assay also supports the loss of the interaction of Sgo1-VE with Swi6 ------- COMMENT: ea1ec138d2671e21 25 8jCPXzj5zy5fAmkgjOmVilFcgGo (Fig. 3c) Accordingly, the replacement of Val 242 with Glu (VE) in Sgo1 abolished the interaction with Swi6 while preserving the interaction with Par1, a subunit of PP2A. An immunoprecipitation assay also supports the loss of the interaction of Sgo1-VE with Swi6 ------- COMMENT: ea1ec138d2671e21 26 8jCPXzj5zy5fAmkgjOmVilFcgGo (Fig. 3c) Accordingly, the replacement of Val 242 with Glu (VE) in Sgo1 abolished the interaction with Swi6 while preserving the interaction with Par1, a subunit of PP2A. An immunoprecipitation assay also supports the loss of the interaction of Sgo1-VE with Swi6 ------- COMMENT: ea1ec138d2671e21 27 xgL7bpBBjPZ02LjqEt2Okf0tMEs (Fig. 3f). The assay of chromosome segregation further revealed that sgo1-VE cells provoke nondisjunction in meiosis II, similarly to swi6D cells ------- COMMENT: ea1ec138d2671e21 28 T5JTW6d7CnOsRUB84XD251+mU7o (Fig. 3f and Supplementary Fig. 8). The Sgo1-VE protein, when fused with CDand thereby localized to the centromere, can perform its full functionin protecting Rec8 ------- COMMENT: ea1ec138d2671e21 29 apaLNc3Vx5TKKLd+6I1Y73uTnYo (Fig. 2a, 2b) As with sgo1D cells, swi6D cells undergo intact meiosis I but suffer a nondisjunction of sister chromatids in meiosis II ------- COMMENT: ea5eb1c311e9f19d 1 Jux13oEDwydGuhCtvNYbUH5fHPM (comment: CHECK sufficient to trigger cell shape change when targeted to cell sides by fusion with Cdr2; tea1/pom1 double mutant phenotype shows that Tea4 role is independent of Pom1) ------- COMMENT: ea5eb1c311e9f19d 2 bTQw7GMReJieqN9cfOmoi7oF9iI (comment: CHECK necessary to trigger cell shape change upon Tea4 targeting to cell sides by fusion with Cdr2) ------- COMMENT: ea5eb1c311e9f19d 3 0fM7Bpy7ibsVlJld+RbZ9lMj754 (comment: CHECK sufficient to trigger cell shape change when targeted to cell sides by fusion with Cdr2) ------- COMMENT: ea5eb1c311e9f19d 4 0fM7Bpy7ibsVlJld+RbZ9lMj754 (comment: CHECK sufficient to trigger cell shape change when targeted to cell sides by fusion with Cdr2) ------- COMMENT: ea5eb1c311e9f19d 5 bTQw7GMReJieqN9cfOmoi7oF9iI (comment: CHECK necessary to trigger cell shape change upon Tea4 targeting to cell sides by fusion with Cdr2) ------- COMMENT: ea75ccdaa88fc91d 1 ngqtK3GzKSM9gzubJH5C97uwMI4 figure 1, Figure 3B, Table 2 ------- COMMENT: ea75ccdaa88fc91d 2 aSH1UoHehaOSoogIzECd67T4pn4 figure 1 ------- COMMENT: ea75ccdaa88fc91d 3 88B/FF9PvhJN9wjH6WXmnFBBq5Q figure 2a ------- COMMENT: ea75ccdaa88fc91d 4 88B/FF9PvhJN9wjH6WXmnFBBq5Q figure 2a ------- COMMENT: ea75ccdaa88fc91d 5 88B/FF9PvhJN9wjH6WXmnFBBq5Q figure 2a ------- COMMENT: ea75ccdaa88fc91d 6 2HRiZ9EuPBSKNrFlpF/yHXmObig figure 1, Figure 3B, Table 2 ------- COMMENT: ea75ccdaa88fc91d 8 4uq4HllgQA4s8+Y17tZFHfTihpQ Figure 4D, lane 3 ------- COMMENT: ea75ccdaa88fc91d 9 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: ea75ccdaa88fc91d 10 xJkr3L43cQrv1CVkG5jw5wnCtZI Figure 4D, lane 3 + figure 5 ------- COMMENT: ea75ccdaa88fc91d 11 VQwyyMBfQueRCgWSq5NNqBnXO6Q Figure 5D ------- COMMENT: ea75ccdaa88fc91d 12 n6oz6nKAXqdO0Qsp1RhzXAKTrTE Figure 5E ------- COMMENT: ea75ccdaa88fc91d 13 qo6VFDz6559eESaaM8/25PRyV5g Figure 5E (comment: to membrane) ------- COMMENT: ea75ccdaa88fc91d 14 74h+cHq+hw9UOLS6/cBhaVFhU7c (Figure 7b) (comment: heterologous complementation) ------- COMMENT: ea75ccdaa88fc91d 15 lO0rewEI7hEvVDspHNwuLN20Erw figure 7b ------- COMMENT: ea75ccdaa88fc91d 16 lO0rewEI7hEvVDspHNwuLN20Erw figure 7b ------- COMMENT: ea75ccdaa88fc91d 17 4WQ7ADqX6Ts+yZuKsX0FU70Wa1A (Figure 7D) (comment: assayed reaction products) ------- COMMENT: ea75ccdaa88fc91d 18 JKhWVuvAoATe8N2pO+d5GYy6uCc (comment: from MF) ------- COMMENT: ea75ccdaa88fc91d 19 JKhWVuvAoATe8N2pO+d5GYy6uCc (comment: from MF) ------- COMMENT: ea75ccdaa88fc91d 21 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: ea75ccdaa88fc91d 22 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: ea7f33ab6887fe6d 77 j9YF5poX7dZ61iuV8t/kNGIzF/Y (comment: not annotated to other stresses as subsequent papers show it is critical for assembly of signaling MAPKKK-MAPKKmodule) ------- COMMENT: ea89a58770744181 1 QoNIDarPzeUSxqIfvqWGOGZedIo trm401 (Trm4a) methylates C34 of tRNA-Leu (CAA) and tRNA-Pro (CGG) as well as all C48 tRNA methylation sites. Methylates C34 only on intron-containing tRNA. ------- COMMENT: ea89a58770744181 3 RVqueaKvX6rPFuLDZFx91MPRVdY abolished tRNA C34, C48 methylation (comment: trna bisulphite sequencing) ------- COMMENT: ea89a58770744181 4 quYPFBB0wLZqHuOx0pflPGpe5qc trm402 (Trm4b) methylates C49 and C50 of tRNAs ------- COMMENT: ea89a58770744181 5 aFCbr6Tn2S8ylxsINIzUK3692SE Abolished tRNA cytosine-5 methylation of C49 and C50 (comment: tRNA bisulphite sequencing) ------- COMMENT: eab9a81ad211e62f 1 LNIxfi2XeaOAm9dHNZJxTJbRA6o data not shown (comment: Non permissive temperature is 32°C and above) ------- COMMENT: eab9a81ad211e62f 2 1XFypcPzm1/J4Y5KFrxb9+16KwU data not shown, (comment: permissive temperature 25°C) ------- COMMENT: eab9a81ad211e62f 3 eRE7t54p7pek8pAkBiiUOa2PtdE Fig1 (comment: permissive temperature is 25°C) ------- COMMENT: eab9a81ad211e62f 4 4+KwXdE3oryL1ZPMm1aiXEy9x5s Fig1. (comment: They describe cells as swollen in their middle region) ------- COMMENT: eab9a81ad211e62f 5 Ci+bKhUitsutwHeHgf5TiETS96Y Fig1 ------- COMMENT: eab9a81ad211e62f 6 XyZXVIT6w2EzHi7nND5rgqOqJD0 Fig1C, Table 2 ------- COMMENT: eab9a81ad211e62f 7 ECEMzFu4FB++QHBbODppNWiOeOU Fig1, Table 2 ------- COMMENT: eab9a81ad211e62f 8 EftTJWMk5K5yy5a1Nog8/ZcjftA Table 2 (comment: This distribution is only seen in cells with a rod shaped appearance) ------- COMMENT: eab9a81ad211e62f 9 EftTJWMk5K5yy5a1Nog8/ZcjftA Table 2 (comment: This distribution is only seen in cells with a rod shaped appearance) ------- COMMENT: eab9a81ad211e62f 10 eVtiANyVPv3aIzBKeX2L/7ZC4tk Table 2 (comment: This distribution is only seen in cells with a rod shaped appearance) ------- COMMENT: eab9a81ad211e62f 11 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: eab9a81ad211e62f 13 xaOfiXN8xQmZM8w1I0aZizmWUyU Fig1B (comment: This distribution is only seen in cells with a rod shaped appearance) ------- COMMENT: eab9a81ad211e62f 14 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: eab9a81ad211e62f 15 YXw2P5RZyTE+TGidHLwJ7A4e0Fo Fig2A, 2B ------- COMMENT: eab9a81ad211e62f 16 YXw2P5RZyTE+TGidHLwJ7A4e0Fo Fig2A, 2B ------- COMMENT: eab9a81ad211e62f 17 /uS0UocOv+bpwiTHE+rAL1BgjXs Fig2D ------- COMMENT: eab9a81ad211e62f 18 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: eab9a81ad211e62f 19 R749BIiSeAnSTi3CN24PvbWQ/04 Fig2A and data not shown ------- COMMENT: eab9a81ad211e62f 21 8oocRGDkbG+AKfzr/mpG7YVeL60 Fig3A, 2B Deletion of the talin domain suppresses the premature activation of bipolar growth in a cdc10 mutant in latA ------- COMMENT: eab9a81ad211e62f 23 Ozp47kvNh5k2BQB6BeY/MCsDsaY Fig4D ------- COMMENT: eab9a81ad211e62f 24 TmEE+klYwkpWI6X34r58BJ+2pNI data for cdc25-22 block not shown but see also Fig4A ------- COMMENT: eab9a81ad211e62f 26 InAoyMdP/QvHvIvvNV5kmSvzrUU Fig 4B ------- COMMENT: eab9a81ad211e62f 27 GJ8kSalMKPIbEiEn8n/VS6NEm7A data not shown, same as Fig 4C ------- COMMENT: eab9a81ad211e62f 28 5zLBcPfE96G7rFQPJBZsaEOa1Og Fig 4C ------- COMMENT: eab9a81ad211e62f 37 O5ChQshWo9SMl9ef8VwaxdF3zLk Fig4B ------- COMMENT: eab9a81ad211e62f 38 R749BIiSeAnSTi3CN24PvbWQ/04 Fig2A and data not shown ------- COMMENT: eab9a81ad211e62f 39 4+KwXdE3oryL1ZPMm1aiXEy9x5s Fig1. (comment: They describe cells as swollen in their middle region) ------- COMMENT: eab9a81ad211e62f 43 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: eabcf681d0ea9788 1 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: eabcf681d0ea9788 2 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: eabcf681d0ea9788 3 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: eabcf681d0ea9788 4 O6flUTaI7T0VNonD0wR9Jcjud8A Phenotype of Erg25 overexpression is suppressed by Erg11 inhibition. Fig. 1E ------- COMMENT: eabcf681d0ea9788 5 4hxGT7pLh8CfRDWZXwMEvfPcDPM Fig. S1C ------- COMMENT: eabcf681d0ea9788 6 FeX3nNhC/gudGSWvsa359Zi1Hgk Fig. S1D ------- COMMENT: eabcf681d0ea9788 7 bQ398RDH40pzL6Cxr4naZ2FYtrU Fig. S1E, S1F ------- COMMENT: eabcf681d0ea9788 8 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: eabcf681d0ea9788 9 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: eabcf681d0ea9788 10 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: eabcf681d0ea9788 11 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: eabcf681d0ea9788 12 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: eabcf681d0ea9788 13 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: eabcf681d0ea9788 14 GiU9OuJkzQhTGzbi+6lSBIDTOUI Fig. 6A, 6B ------- COMMENT: eabcf681d0ea9788 15 mKHrmdoHpr4rPKS2pKRyXvNdG7s Fig. S6 ------- COMMENT: eabcf681d0ea9788 16 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: eabcf681d0ea9788 17 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: eabcf681d0ea9788 18 mc5vZAZlJJNaNb0FCzgVif7dp0c Fig. 5A ------- COMMENT: eabcf681d0ea9788 19 xLld+U4XtO1Yq5XJ/vA+lPgihjU Fig. 6D ------- COMMENT: eabcf681d0ea9788 20 xLld+U4XtO1Yq5XJ/vA+lPgihjU Fig. 6D ------- COMMENT: eaf06b3b341808fa 1 d3Xse7cGPWDuwzzqs/nAUXC/GWQ (comment: CHECK increased spatial extent of heterochromatin assembly) (comment: JUST, not at prpote4in coding gene!) ------- COMMENT: eaf06b3b341808fa 2 +V9U5P9kFoH6d8PGF44MRJm+fAE (comment: outer repeats) ------- COMMENT: eaf06b3b341808fa 3 2dRiWjKyUFpX0JbsWX6z3L5de3g (comment: CHECK increased spatial extent of heterochromatin assembly) (comment: JUST) ------- COMMENT: eaf06b3b341808fa 4 B60JyeO/4ScIdaxhw74Uds/JS3c (comment: CHECK normal spatial extent of heterochromatin assembly (comment: JUST) ------- COMMENT: eaf06b3b341808fa 7 HPkNhAnPgvjhxwR1u4+CH2PivZE Figure 6A, 6B ------- COMMENT: eaf06b3b341808fa 8 vmEJiaZHWs8NOGPIR/b25oDdqHU Figure 6C ------- COMMENT: eaf06b3b341808fa 9 vmEJiaZHWs8NOGPIR/b25oDdqHU Figure 6C ------- COMMENT: eaf06b3b341808fa 10 vmEJiaZHWs8NOGPIR/b25oDdqHU Figure 6C ------- COMMENT: eaf06b3b341808fa 11 vmEJiaZHWs8NOGPIR/b25oDdqHU Figure 6C ------- COMMENT: eaf900e1140bdecb 1 QSu9FHaiQeG1IHMv2OL1d1b9i6I (comment: localization dependent on F-actin (assayed using Latrunculin A)) ------- COMMENT: eb3460dd813ce225 1 AV4sed/IC1vRYOTKrlvQxd1S8BE An arginine substitution of Ile379 or Leu383 of Taz1 or Ile655 of SpRap1 at the center of the hydrophobic interface completely abolished the Taz1RBM-SpRap1RCT interaction (Fig. 6a). ------- COMMENT: eb3460dd813ce225 2 AV4sed/IC1vRYOTKrlvQxd1S8BE An arginine substitution of Ile379 or Leu383 of Taz1 or Ile655 of SpRap1 at the center of the hydrophobic interface completely abolished the Taz1RBM-SpRap1RCT interaction (Fig. 6a). ------- COMMENT: eb3460dd813ce225 3 AV4sed/IC1vRYOTKrlvQxd1S8BE An arginine substitution of Ile379 or Leu383 of Taz1 or Ile655 of SpRap1 at the center of the hydrophobic interface completely abolished the Taz1RBM-SpRap1RCT interaction (Fig. 6a). ------- COMMENT: eb3460dd813ce225 4 AV4sed/IC1vRYOTKrlvQxd1S8BE An arginine substitution of Ile379 or Leu383 of Taz1 or Ile655 of SpRap1 at the center of the hydrophobic interface completely abolished the Taz1RBM-SpRap1RCT interaction (Fig. 6a). ------- COMMENT: eb3460dd813ce225 5 AV4sed/IC1vRYOTKrlvQxd1S8BE An arginine substitution of Ile379 or Leu383 of Taz1 or Ile655 of SpRap1 at the center of the hydrophobic interface completely abolished the Taz1RBM-SpRap1RCT interaction (Fig. 6a). ------- COMMENT: eb3460dd813ce225 6 AV4sed/IC1vRYOTKrlvQxd1S8BE An arginine substitution of Ile379 or Leu383 of Taz1 or Ile655 of SpRap1 at the center of the hydrophobic interface completely abolished the Taz1RBM-SpRap1RCT interaction (Fig. 6a). ------- COMMENT: eb3460dd813ce225 7 AV4sed/IC1vRYOTKrlvQxd1S8BE An arginine substitution of Ile379 or Leu383 of Taz1 or Ile655 of SpRap1 at the center of the hydrophobic interface completely abolished the Taz1RBM-SpRap1RCT interaction (Fig. 6a). ------- COMMENT: eb3460dd813ce225 8 JsfIQn3e4S4gSm8L6TlPKcb5jYA Consistent with the published results, deletion of taz1+ or rap1+ from yeast cells resulted in a dramatic increase in telomere length and length heterogeneity compared to wild-type cells (Fig. 6c). ------- COMMENT: eb3460dd813ce225 10 kvST4ddIBtZJ59dKo0AOn3/BX7g Three point mutants (Taz1 I379R, Taz1 L383R, and Rap1 I655R) that completely abolished the Taz1-SpRap1 interaction in the ITC assay displayed a rap1Δ– and taz1Δ-like telomere length defect (Figs. 6b and 6c) ------- COMMENT: eb3460dd813ce225 13 s0A7fGG73/tuzrzK7nuz5hgu/C4 Three mutants (Taz1 I379R, Taz1 L383R, and Rap1 I655R) with no detectable Taz1-SpRap1 interaction clearly exhibited altered mobility bands representing intra-chromosome fusions (Fig. 6e). ------- COMMENT: eb4340d44f575950 28 P8qLmef4vtYrF+1naWdtdadzRos (comment: CHECK inhibited by CCCP) ------- COMMENT: ebbf99a628743442 1 q7yQ4AbC2bEj6KiCpphVWV06cCo dns ------- COMMENT: ebc037f099adf95b 18 9jGMzJtJqQHQx9ec9ey0cU4ZbbM (comment: CHECK YES, YES (low-glucose)) ------- COMMENT: ebc037f099adf95b 19 9jGMzJtJqQHQx9ec9ey0cU4ZbbM (comment: CHECK YES, YES (low-glucose)) ------- COMMENT: ebc037f099adf95b 96 XfgE2Kz2n02iHoO/V+3wy8dOGOw (comment: ALERTED FROM A LATER PAPER) Rst2 regulates the expression of ste11, which encodes a transcription factor to regulate sexual development (Sugimoto et al. 1991); fbp1, which encodes fructose-1,6-bisphosphatase (Hoffman and Winston 1990); and mug14, which encodes an adducin homolog (Inamura et al. 2021). ------- COMMENT: ebd6c4def00a2f50 5 YXHGFHU5LadekPlbrwHvPt7VWDU (Fig. 4) ------- COMMENT: ebd6c4def00a2f50 6 YXHGFHU5LadekPlbrwHvPt7VWDU (Fig. 4) ------- COMMENT: ebd6c4def00a2f50 7 YXHGFHU5LadekPlbrwHvPt7VWDU (Fig. 4) ------- COMMENT: ebd6c4def00a2f50 8 YXHGFHU5LadekPlbrwHvPt7VWDU (Fig. 4) ------- COMMENT: ebd6c4def00a2f50 9 YXHGFHU5LadekPlbrwHvPt7VWDU (Fig. 4) ------- COMMENT: ebd6c4def00a2f50 10 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: ebd6c4def00a2f50 11 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: ebd6c4def00a2f50 14 gZNo/xWKvKsHWWGOazkQEqseH2U (Fig. 1) ------- COMMENT: ebd6c4def00a2f50 15 gZNo/xWKvKsHWWGOazkQEqseH2U (Fig. 1) ------- COMMENT: ebd6c4def00a2f50 16 gZNo/xWKvKsHWWGOazkQEqseH2U (Fig. 1) ------- COMMENT: ebd6c4def00a2f50 17 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 18 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 19 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 20 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 21 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 22 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 23 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 24 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 25 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 26 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 27 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 28 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 29 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 30 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 31 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 32 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 33 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: ebd6c4def00a2f50 34 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: ebd6c4def00a2f50 35 xhsEwSjsS3WiGn8vmKaT8Vr1lUo (Fig. S2) ------- COMMENT: ebd6c4def00a2f50 36 xhsEwSjsS3WiGn8vmKaT8Vr1lUo (Fig. S2) ------- COMMENT: ebd6c4def00a2f50 37 xhsEwSjsS3WiGn8vmKaT8Vr1lUo (Fig. S2) ------- COMMENT: ebd6c4def00a2f50 38 xhsEwSjsS3WiGn8vmKaT8Vr1lUo (Fig. S2) ------- COMMENT: ebd6c4def00a2f50 39 xhsEwSjsS3WiGn8vmKaT8Vr1lUo (Fig. S2) ------- COMMENT: ebd6c4def00a2f50 40 xhsEwSjsS3WiGn8vmKaT8Vr1lUo (Fig. S2) ------- COMMENT: ebd6c4def00a2f50 41 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: ebd6c4def00a2f50 42 PEijj9MIrNSBSecC4MlXqO1v4Zk (Fig. 3) ------- COMMENT: ebd6c4def00a2f50 43 PEijj9MIrNSBSecC4MlXqO1v4Zk (Fig. 3) ------- COMMENT: ebd6c4def00a2f50 44 PEijj9MIrNSBSecC4MlXqO1v4Zk (Fig. 3) ------- COMMENT: ebd6c4def00a2f50 45 PEijj9MIrNSBSecC4MlXqO1v4Zk (Fig. 3) ------- COMMENT: ebd6c4def00a2f50 46 PEijj9MIrNSBSecC4MlXqO1v4Zk (Fig. 3) ------- COMMENT: ebd6c4def00a2f50 47 PEijj9MIrNSBSecC4MlXqO1v4Zk (Fig. 3) ------- COMMENT: ebd6c4def00a2f50 48 PEijj9MIrNSBSecC4MlXqO1v4Zk (Fig. 3) ------- COMMENT: ebd6c4def00a2f50 49 PEijj9MIrNSBSecC4MlXqO1v4Zk (Fig. 3) ------- COMMENT: ebd6c4def00a2f50 52 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 53 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 54 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 55 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 56 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 57 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 58 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 59 2ZBcYRNShURyrVc3i6ot5nSBLLI (Fig. 2A, 2B) ------- COMMENT: ebd6c4def00a2f50 60 PEijj9MIrNSBSecC4MlXqO1v4Zk (Fig. 3) ------- COMMENT: ebd6c4def00a2f50 61 YXHGFHU5LadekPlbrwHvPt7VWDU (Fig. 4) ------- COMMENT: ebd6c4def00a2f50 62 YXHGFHU5LadekPlbrwHvPt7VWDU (Fig. 4) ------- COMMENT: ebd6c4def00a2f50 63 YXHGFHU5LadekPlbrwHvPt7VWDU (Fig. 4) ------- COMMENT: ebd6c4def00a2f50 64 YXHGFHU5LadekPlbrwHvPt7VWDU (Fig. 4) ------- COMMENT: ebd6c4def00a2f50 65 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: ebd6c4def00a2f50 66 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: ebd6c4def00a2f50 67 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: ebd6c4def00a2f50 68 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: ebd6c4def00a2f50 69 xhsEwSjsS3WiGn8vmKaT8Vr1lUo (Fig. S2) ------- COMMENT: ebd6c4def00a2f50 70 xhsEwSjsS3WiGn8vmKaT8Vr1lUo (Fig. S2) ------- COMMENT: ebd6c4def00a2f50 71 xhsEwSjsS3WiGn8vmKaT8Vr1lUo (Fig. S2) ------- COMMENT: ebd6c4def00a2f50 72 BlZAYgg6W/+/O+S+HMuoElV4g98 (Fig. S6A) ------- COMMENT: ebd6c4def00a2f50 73 BlZAYgg6W/+/O+S+HMuoElV4g98 (Fig. S6A) ------- COMMENT: ebd6c4def00a2f50 74 SamTvRZvEvQIrgSzuqc8al8jR1s (Fig. S6B) ------- COMMENT: ebd6c4def00a2f50 75 SamTvRZvEvQIrgSzuqc8al8jR1s (Fig. S6B) ------- COMMENT: ebd6c4def00a2f50 76 BlZAYgg6W/+/O+S+HMuoElV4g98 (Fig. S6A) ------- COMMENT: ebd6c4def00a2f50 77 BlZAYgg6W/+/O+S+HMuoElV4g98 (Fig. S6A) ------- COMMENT: ebd6c4def00a2f50 78 BlZAYgg6W/+/O+S+HMuoElV4g98 (Fig. S6A) ------- COMMENT: ebd6c4def00a2f50 79 BlZAYgg6W/+/O+S+HMuoElV4g98 (Fig. S6A) ------- COMMENT: ebd6c4def00a2f50 80 SamTvRZvEvQIrgSzuqc8al8jR1s (Fig. S6B) ------- COMMENT: ebd6c4def00a2f50 81 SamTvRZvEvQIrgSzuqc8al8jR1s (Fig. S6B) ------- COMMENT: ebd7df89af69aecb 2 UUDKNtq+Gk30UMnsvpE75xH+XEM (comment: Expression level up 35 times) ------- COMMENT: ebd7df89af69aecb 3 K9y5dbjZxq1xp5a7fQnFTfkv1Fs (comment: Expression level up 8 times.) ------- COMMENT: ebd7df89af69aecb 4 5+6UR+XqwzPIIQ2vn14V92H97io (comment: Expression level up 22 times) ------- COMMENT: ebd7df89af69aecb 5 n4E+XErIO6t2bii4WWDcVCR5EuE (comment: Expression level up 31 times) ------- COMMENT: ebd7df89af69aecb 6 qCYQWhbzKJ/UD8YxaiR84TFfOyg (comment: Expression level up 43 times) ------- COMMENT: ebd7df89af69aecb 7 kPuVF/G+Xs7AMYAJoi3ixdWwtFc (comment: Expression level up 38 times) ------- COMMENT: ebd7df89af69aecb 8 1tWQcNIFJDr+bHG/8RTk2D4wl60 (comment: Expression level up 23 times) ------- COMMENT: ebd7df89af69aecb 9 NDz8kZ2VmzM79H0F46C/gk+2Y9w (comment: Expression level up 25 times) ------- COMMENT: ebd7df89af69aecb 11 +n76/bDgJPwsQ85sCdv3aEapp0g (comment: Expression level up 2 times) ------- COMMENT: ebd7df89af69aecb 12 +n76/bDgJPwsQ85sCdv3aEapp0g (comment: Expression level up 2 times) ------- COMMENT: ebd7df89af69aecb 13 +n76/bDgJPwsQ85sCdv3aEapp0g (comment: Expression level up 2 times) ------- COMMENT: ebd7df89af69aecb 14 GS7BjpkBGht9CHTYFwNxacyj2qM (comment: Expression level up 3 times) ------- COMMENT: ebd7df89af69aecb 15 +n76/bDgJPwsQ85sCdv3aEapp0g (comment: Expression level up 2 times) ------- COMMENT: ebd7df89af69aecb 16 GG4nsuAplaaeK99IN1GMqK7i9z8 (comment: Expression level up 2.5 times) ------- COMMENT: ec4393738ec076b0 1 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: ec4393738ec076b0 2 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: ec4393738ec076b0 3 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: ec4393738ec076b0 4 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: ec4393738ec076b0 5 ZA1Bp+wpf96G0pav60kAVh8AbTI (comment: A1 cleavage) ------- COMMENT: ec4393738ec076b0 7 jmzVrYiMFzRsLqSfD2izIrAWKZM (comment: A2 cleavage) ------- COMMENT: ec4393738ec076b0 8 qJx5o0VjgSoqdEHM3hIOU29J894 (comment: A0 cleavage) ------- COMMENT: ec4424d3ed6a4dc1 1 df5YwK93X7CIs2aPzSRWQgdjylM (comment: crystal structure) ------- COMMENT: ec5bea036f16d96e 1554 NAQgUb2ywUuoeyWTlXi7TcsiKS8 (comment: ChIP-seq) ------- COMMENT: ec5bea036f16d96e 1555 NAQgUb2ywUuoeyWTlXi7TcsiKS8 (comment: ChIP-seq) ------- COMMENT: ec5bea036f16d96e 1570 NAQgUb2ywUuoeyWTlXi7TcsiKS8 (comment: ChIP-seq) ------- COMMENT: ec5bea036f16d96e 1571 NAQgUb2ywUuoeyWTlXi7TcsiKS8 (comment: ChIP-seq) ------- COMMENT: ec5bea036f16d96e 1572 NAQgUb2ywUuoeyWTlXi7TcsiKS8 (comment: ChIP-seq) ------- COMMENT: ec5bea036f16d96e 1573 NAQgUb2ywUuoeyWTlXi7TcsiKS8 (comment: ChIP-seq) ------- COMMENT: ec5bea036f16d96e 1574 NAQgUb2ywUuoeyWTlXi7TcsiKS8 (comment: ChIP-seq) ------- COMMENT: ec5bea036f16d96e 1575 NAQgUb2ywUuoeyWTlXi7TcsiKS8 (comment: ChIP-seq) ------- COMMENT: ec5bea036f16d96e 1576 NAQgUb2ywUuoeyWTlXi7TcsiKS8 (comment: ChIP-seq) ------- COMMENT: ec5bea036f16d96e 1577 NAQgUb2ywUuoeyWTlXi7TcsiKS8 (comment: ChIP-seq) ------- COMMENT: ec5bea036f16d96e 1580 NAQgUb2ywUuoeyWTlXi7TcsiKS8 (comment: ChIP-seq) ------- COMMENT: ec5bea036f16d96e 1581 NAQgUb2ywUuoeyWTlXi7TcsiKS8 (comment: ChIP-seq) ------- COMMENT: ec5bea036f16d96e 1586 JRvjTB5GU2m34xxxkWGmTu44xUc (comment: ChIP-seq; same severity as spt16-1 alone) ------- COMMENT: ec5bea036f16d96e 1587 NAQgUb2ywUuoeyWTlXi7TcsiKS8 (comment: ChIP-seq) ------- COMMENT: ec5bea036f16d96e 1600 NAQgUb2ywUuoeyWTlXi7TcsiKS8 (comment: ChIP-seq) ------- COMMENT: ec5bea036f16d96e 1601 NAQgUb2ywUuoeyWTlXi7TcsiKS8 (comment: ChIP-seq) ------- COMMENT: ec5bea036f16d96e 1604 NAQgUb2ywUuoeyWTlXi7TcsiKS8 (comment: ChIP-seq) ------- COMMENT: ec5bea036f16d96e 1605 NAQgUb2ywUuoeyWTlXi7TcsiKS8 (comment: ChIP-seq) ------- COMMENT: ec98ab47f2b47ef1 1 9hrvdAllmBLddfo0riewa4KzUKo The peroxidase activity of BCP (bacterioferritin comigratory protein) was similar to that of TPx. ------- COMMENT: ecbfda73cbbd469b 1 7a0JLTLphQZvahSUwRVuQHgDQfo Rad3-dependent phosphorylation of Chk1 is observed in the presence of phleomycin, but this is suppressed by KCl concentrations above 0.3 M ------- COMMENT: ecbfda73cbbd469b 11 OJHttL6mH1Mj0AVCS7E/dEzQeyo (comment: CONDITION 100 ug/ml G418 for 4 h followed by recovery on YES) ------- COMMENT: ecbfda73cbbd469b 12 JIaljIEJOBxBKCSPLxaRYPqMi88 (comment: CONDITION 10 ug/ml phleomycin for 4 h followed by recovery on YES) ------- COMMENT: ecbfda73cbbd469b 13 4JVaBBbTziO1Sm5YP4kJzfw1vcU (comment: CONDITION Sensitivity was rescued by 0.6 M KCl 4 mM lithium for 4 h followed by recovery on YES) ------- COMMENT: ecbfda73cbbd469b 14 cX3wiMZDK3F4cMbnLS4djEpQS20 (comment: CONDITION Sensitivity was rescued by 0.6 M KCl, 10 ug/ml phleomycin for 4 h followed by recovery on YES) ------- COMMENT: ecbfda73cbbd469b 15 ykcFPvrqad575dd+nGvu2NlMuVY (comment: CONDITION 10 ug/ml G418 for 4 h followed by recovery on YES) ------- COMMENT: ecbfda73cbbd469b 16 UU7IbZrX20yfRntlp6FLjLzE6k4 (comment: CONDITION Sensitivity was rescued by 0.6 M KCl, 40 ug/ml doxorubicin for 4 h followed by recovery on YES) ------- COMMENT: ecbfda73cbbd469b 17 mGRZZV4Mv2LX+vlWr7Mdcr8NaOw (comment: CONDITION Sensitivity was rescued by 150 mM, but not 70 mM KCl, 10 ug/ml phleomycin for 4 h followed by recovery on YES) ------- COMMENT: ecbfda73cbbd469b 18 uV4Is/NlKetbQGMISjoBAqg+bs4 (comment: CONDITION Sensitivity was rescued by 0.6 M, but not 60 mM KCl, 40 ug/ml doxorubicin for 4 h followed by recovery on YES) ------- COMMENT: ecbfda73cbbd469b 19 ZKGlw5BKU0ENRoWwRTkNzAp2Ex4 (comment: CONDITION Sensitivity was rescued by 0.6 M KCl but not 60 mM KCl, 10 ug/ml phleomycin for 4 h followed by recovery on YES) ------- COMMENT: ecbfda73cbbd469b 26 5MYx3StM9prXhZq6IGt4JK7uShM (comment: CONDITION Sensitivity was rescued less efficiently than for the wt by 150 - 600 mM KCl) ------- COMMENT: ecdb1fc29168829b 1 L8rivm19XcdtPF/Ijfu+dYR4UXs In an asynchronously growing culture, Sor1- GFP localized to the nucleus in most cells (Supplementary Fig. 2a) ------- COMMENT: ecdb1fc29168829b 3 TEzE6HmEiOznvcFa+mBWblqSV+A In metaphase, sor1Δ mutant cells showed a small, but significant, increase of split sister centromeres (Fig. 2a), indicative of a cohesion defect between sister centromeres. ------- COMMENT: ecdb1fc29168829b 4 BSKGPIB3SOrZhCX8qNjikTuFZ18 Interestingly, the increase in split sister centromeres in sor1Δ mutant cells was prevented in sor1Δ wpl1Δ double mutants (compared to wild type), suggesting that similarly to mammalian cells wpl1 deletion reduces the sister chromatid cohesion defect caused by the sor1Δ mutation (Fig. 2a). ------- COMMENT: ecdb1fc29168829b 5 p6iDLg2QpRLyRYZOEgtq1UvAvYU Indeed, we observed a higher frequency of lagging chromosomes associated with a higher rate of chromosome mis-segregation in eso1-G799D sor1Δ and mis4-242 sor1Δ double mutants as compared to single mutants (Fig. 2b). ------- COMMENT: ecdb1fc29168829b 6 p6iDLg2QpRLyRYZOEgtq1UvAvYU Indeed, we observed a higher frequency of lagging chromosomes associated with a higher rate of chromosome mis-segregation in eso1-G799D sor1Δ and mis4-242 sor1Δ double mutants as compared to single mutants (Fig. 2b). ------- COMMENT: ecdb1fc29168829b 7 NnM7L/yijzF4tg3UXY486ZydmI8 We indeed observed that Pds5-Myc co-immunoprecipitated with Sor1-Pk and Sor1-Pk co-immunoprecipitated with Psm3-GFP (Fig. 2c, d). ------- COMMENT: ecdb1fc29168829b 8 NnM7L/yijzF4tg3UXY486ZydmI8 We indeed observed that Pds5-Myc co-immunoprecipitated with Sor1-Pk and Sor1-Pk co-immunoprecipitated with Psm3-GFP (Fig. 2c, d). ------- COMMENT: ecdb1fc29168829b 9 byVKDHoFrb2CETvHvOXoORoctdk Sor1-D303A-Pk co-immunoprecipitated less efficiently with the Psm3-GFP protein, compared to wild-type Sor1- Pk, suggesting that the conserved residue D303 in the Sororin domain of Sor1 is important for the association of Sor1 with cohesin (Fig. 2d). ------- COMMENT: ecdb1fc29168829b 10 by62/FY4qGatV+Oll/sWGPdwd60 (comment: referring to growth defect) Mutating three other conserved residues in the Sororin domain of Sor1 (F299A, V302A and Y305A) resulted in a similar phenotype (Fig. 2e). ------- COMMENT: ecdb1fc29168829b 11 by62/FY4qGatV+Oll/sWGPdwd60 (comment: referring to growth defect) Mutating three other conserved residues in the Sororin domain of Sor1 (F299A, V302A and Y305A) resulted in a similar phenotype (Fig. 2e). ------- COMMENT: ecdb1fc29168829b 12 by62/FY4qGatV+Oll/sWGPdwd60 (comment: referring to growth defect) Mutating three other conserved residues in the Sororin domain of Sor1 (F299A, V302A and Y305A) resulted in a similar phenotype (Fig. 2e). ------- COMMENT: ecdd339b2776e271 1 1XMTVwSq0WHPVk6APEzyMQuglo0 Figure 6C, 6D Increased mitochondrial numbers and decreased mitochondrial sizes with overall mitochondrial volume same as what is observed in wild-type cells ------- COMMENT: ecdd339b2776e271 2 Z1Y/p0tabjqd/jyXomCdDmknQQg Figure 1C, 1D ------- COMMENT: ecdd339b2776e271 3 5Leuj0nQtQ/oH58v8XLkYXSDMog Figure 6B ------- COMMENT: ecdd339b2776e271 4 wU+fX57/HDFQMNL8WNeO4cS0/xU Figure 1B the anti-parallel microtubule bundles are only about half the length of wild-type bundles ------- COMMENT: ecdd339b2776e271 5 aU2gJmucr3fgTFZ5lASje+bU1Iw Figure 2C the mitochondria have a fission frequency that is almost double that of wild-type ------- COMMENT: ecdd339b2776e271 8 FTrXtJ5zQNg2J4MTphOudFDWbkk Fig 1C observed that Klp5􏰀/Klp6􏰀 contained only 2.3 􏰁 0.4 (mean 􏰁 S.E.). ------- COMMENT: ecdd339b2776e271 9 kUt+fwwm+9FdhlUBMfqNX58inYA Klp5􏰀/Klp6􏰀 cells exhibited a fission frequency that was half that of WT ------- COMMENT: ecdd339b2776e271 12 8qtmH6dN22g9U+D9Kj7dsw/8sVE (Fig. S5B) Furthermore, in Klp4􏰀 cells, which typically contain sev- eral short mitochondria (Fig. 1A), absence of Dnm1 results in a single large, fused mitochondrion ------- COMMENT: ecdd339b2776e271 13 KlaVQllEwxogI8iXoseGlGyNAj4 WT cells highly overex- pressing Dnm1 had 11.6 􏰁 0.2 mitochondria (mean 􏰁 S.E.), which is twice that of WT cells ------- COMMENT: ecdd339b2776e271 14 KsLHmGHnpXsZGKj65FZ2ID3iCzc We counted 23.3 􏰁 1.4 (mean 􏰁 S.E.) mitochondria in Klp5􏰀/Klp6􏰀 cells lacking Mmb1􏰀 (Fig. 6C), which was not significantly different from Mmb1􏰀 cells ------- COMMENT: ece9176a66feeefa 2 PlNQW2QqNz+wDjZYoY4G0SEXQaA (comment: CONDITION 33 degrees) (comment: may be standard for them) ------- COMMENT: ece9176a66feeefa 7 PlNQW2QqNz+wDjZYoY4G0SEXQaA (comment: CONDITION 33 degrees) (comment: may be standard for them) ------- COMMENT: ece9176a66feeefa 12 PlNQW2QqNz+wDjZYoY4G0SEXQaA (comment: CONDITION 33 degrees (comment: may be standard for them) ------- COMMENT: ece9176a66feeefa 16 PlNQW2QqNz+wDjZYoY4G0SEXQaA (comment: CONDITION 33 degrees) (comment: may be standard for them) ------- COMMENT: ece9176a66feeefa 25 PlNQW2QqNz+wDjZYoY4G0SEXQaA (comment: CONDITION 33 degrees) (comment: may be standard for them) ------- COMMENT: ece9176a66feeefa 26 PlNQW2QqNz+wDjZYoY4G0SEXQaA (comment: CONDITION 33 degrees) (comment: may be standard for them) ------- COMMENT: ece9176a66feeefa 52 gTpLgShfLrCsFhXLMw0HGBAyaK0 (comment: CONDITION 33 degrees) (comment: may be standard for them); (comment: CHECK morphology same as ppe1delta alone) ------- COMMENT: ecfa7f1ddeb87155 1 5l/nTZPsffBwjxhmb19FXbbpjDw (comment: CHECK defective in microtubule growth in the alp14-26 background) ------- COMMENT: ecfa7f1ddeb87155 2 Mc1Dl/8ePjG+W3BQIevC1vaEvqw Figure 2D ------- COMMENT: ecfa7f1ddeb87155 5 b7654cqbEfH/EsiYQaxfdHfvkjg (comment: CHECK defective in microtubule growth during both interphase and mitosis) ------- COMMENT: ecfa7f1ddeb87155 6 YokFC9Xz/Oh54gn8kQjiyrvrNGI appears to retain normal microtubule nucleation activity ------- COMMENT: ecfa7f1ddeb87155 7 E6oMqphkAQqL+ON27pvHh2wecH8 (comment: CHECK temperature sensitive only in the absence of Dis1) ------- COMMENT: ecfa7f1ddeb87155 12 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: ecfa7f1ddeb87155 13 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: ecfa7f1ddeb87155 14 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: ecfa7f1ddeb87155 15 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: ecfa7f1ddeb87155 16 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: ed309cb97e2a45db 1 4C7a0tMlPkBdmoOlliEfK7ln5SQ (comment: sulphate) ------- COMMENT: ed5a1aa8c1ecb938 9 RpU7JKimqUZ5WoPw1ggXxKcL6GI (comment: CHECK residue S604) ------- COMMENT: ed5a1aa8c1ecb938 13 RpU7JKimqUZ5WoPw1ggXxKcL6GI (comment: CHECK residue S604) ------- COMMENT: ed5a1aa8c1ecb938 38 Rs0jjqy3RTKdhIdOHA16Z/odTdw (comment: CHECK in presence of hydroxyurea) ------- COMMENT: ed5a1aa8c1ecb938 43 Rs0jjqy3RTKdhIdOHA16Z/odTdw (comment: CHECK in presence of hydroxyurea) ------- COMMENT: ed5a1aa8c1ecb938 44 onsVYJPa7zYqOj9TGwJ/L6I2sXs phenotype indicates that mrc1/Phos:S604 has higher affinity for chromatin than Unphos:S604 ------- COMMENT: ed6002ed7638d5e4 1 9obkRBahnsvfuKPq+LTlya+asC4 (Fig. 6E) ------- COMMENT: ed6002ed7638d5e4 2 9obkRBahnsvfuKPq+LTlya+asC4 (Fig. 6E) ------- COMMENT: ed6002ed7638d5e4 4 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: ed6002ed7638d5e4 6 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: ed6002ed7638d5e4 7 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: ed6002ed7638d5e4 8 BlZAYgg6W/+/O+S+HMuoElV4g98 (Fig. S6A) ------- COMMENT: ed6002ed7638d5e4 9 BlZAYgg6W/+/O+S+HMuoElV4g98 (Fig. S6A) ------- COMMENT: ed6002ed7638d5e4 10 jTsQneiFN2O87OgZ9cPB1YzCYVU (Fig. S7A) ------- COMMENT: ed6002ed7638d5e4 11 KLOzvR5EIb1BMigGZXVDVLWmsW0 (Fig. S7B) ------- COMMENT: ed6002ed7638d5e4 12 SvlFFz9CaCJByQRBTLvdFnPqSYg (Fig. 1F) ------- COMMENT: ed6002ed7638d5e4 13 UecLDdbzEF2Fd34z5as/4/0I/eg (Fig. S1E) ------- COMMENT: ed6002ed7638d5e4 14 UecLDdbzEF2Fd34z5as/4/0I/eg (Fig. S1E) ------- COMMENT: ed6002ed7638d5e4 15 prmtlQDYLee+WD05YM58owCnL64 (Fig. S7E) ------- COMMENT: ed6002ed7638d5e4 16 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: ed6002ed7638d5e4 17 71ytXNMmrfZhi8SNJsA3yiVrMVc (Fig. 1E, Fig. 6A, 6E) ------- COMMENT: ed6002ed7638d5e4 18 UecLDdbzEF2Fd34z5as/4/0I/eg (Fig. S1E) ------- COMMENT: ed6002ed7638d5e4 19 prmtlQDYLee+WD05YM58owCnL64 (Fig. S7E) ------- COMMENT: ed6002ed7638d5e4 22 bLmE8UPDTdmI/f/9a2LtNFsl3Z8 (Fig. S1G) ------- COMMENT: ed6002ed7638d5e4 23 m7f9LpZJEI+U0fFKsx7mlaXFe4c Our study reveals that as a binding protein for the histone H3–H4 dimer, Djc9 acts with Hsp70 to promote the proteasomal degradation of excess histones. Its activity is restrained by the competitive binding of H3–H4 by Asf1, whose essential function in fission yeast is restricting histone degradation by Djc9. (Fig. 5 and Fig. 6) ------- COMMENT: ed6002ed7638d5e4 24 W4xkP4H/sC1fm9WHOucBJfKfR7Y (Fig. 2) ------- COMMENT: ed6002ed7638d5e4 25 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: ed6002ed7638d5e4 26 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: ed6002ed7638d5e4 27 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: ed6002ed7638d5e4 28 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: ed6002ed7638d5e4 29 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: ed6002ed7638d5e4 30 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: ed6002ed7638d5e4 31 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: ed6002ed7638d5e4 32 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: ed6002ed7638d5e4 33 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: ed6002ed7638d5e4 34 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: ed6002ed7638d5e4 35 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: ed6002ed7638d5e4 36 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: ed6002ed7638d5e4 37 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: ed6002ed7638d5e4 38 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: ed6002ed7638d5e4 39 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: ed6002ed7638d5e4 40 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: ed6002ed7638d5e4 41 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: ed6002ed7638d5e4 42 SvlFFz9CaCJByQRBTLvdFnPqSYg (Fig. 1F) ------- COMMENT: ed6002ed7638d5e4 43 SvlFFz9CaCJByQRBTLvdFnPqSYg (Fig. 1F) ------- COMMENT: ed6002ed7638d5e4 44 SvlFFz9CaCJByQRBTLvdFnPqSYg (Fig. 1F) ------- COMMENT: ed6002ed7638d5e4 45 SvlFFz9CaCJByQRBTLvdFnPqSYg (Fig. 1F) ------- COMMENT: ed6002ed7638d5e4 47 RAQ9iCj6ucjGCQHQQosHYrKMC00 (Fig. 3I) ------- COMMENT: ed6002ed7638d5e4 49 d8xLp/dA0M0C7hDu7v7MWXmIJX4 (Fig. 4C, 4D) ------- COMMENT: ed6002ed7638d5e4 50 d8xLp/dA0M0C7hDu7v7MWXmIJX4 (Fig. 4C, 4D) ------- COMMENT: ed6002ed7638d5e4 51 lzDpBhI+e1KR8hAG7s00IgdEh2I (Fig. 5A) ------- COMMENT: ed6002ed7638d5e4 52 lzDpBhI+e1KR8hAG7s00IgdEh2I (Fig. 5A) ------- COMMENT: ed6002ed7638d5e4 53 lzDpBhI+e1KR8hAG7s00IgdEh2I (Fig. 5A) ------- COMMENT: ed6002ed7638d5e4 54 lzDpBhI+e1KR8hAG7s00IgdEh2I (Fig. 5A) ------- COMMENT: ed6002ed7638d5e4 55 DVJMM6AvJyLz8J8dkwtY+pFizxc (Fig. 5E) ------- COMMENT: ed6002ed7638d5e4 56 DVJMM6AvJyLz8J8dkwtY+pFizxc (Fig. 5E) ------- COMMENT: ed6002ed7638d5e4 57 qgy2UtS49Rc8vkPrVMir2qiYd3M (Fig. 5F) ------- COMMENT: ed6002ed7638d5e4 58 qgy2UtS49Rc8vkPrVMir2qiYd3M (Fig. 5F) ------- COMMENT: ed6002ed7638d5e4 59 qgy2UtS49Rc8vkPrVMir2qiYd3M (Fig. 5F) ------- COMMENT: ed6002ed7638d5e4 60 sUCS79Rs9xFD1wViTRlsgeGdNFs (Fig. 6B) ------- COMMENT: ed6002ed7638d5e4 61 sUCS79Rs9xFD1wViTRlsgeGdNFs (Fig. 6B) ------- COMMENT: ed6002ed7638d5e4 62 9obkRBahnsvfuKPq+LTlya+asC4 (Fig. 6E) ------- COMMENT: ed6002ed7638d5e4 63 UecLDdbzEF2Fd34z5as/4/0I/eg (Fig. S1E) ------- COMMENT: ed6002ed7638d5e4 64 UecLDdbzEF2Fd34z5as/4/0I/eg (Fig. S1E) ------- COMMENT: ed6002ed7638d5e4 65 bLmE8UPDTdmI/f/9a2LtNFsl3Z8 (Fig. S1G) ------- COMMENT: ed6002ed7638d5e4 66 bLmE8UPDTdmI/f/9a2LtNFsl3Z8 (Fig. S1G) ------- COMMENT: ed6002ed7638d5e4 67 RAQ9iCj6ucjGCQHQQosHYrKMC00 (Fig. 3I) ------- COMMENT: ed6002ed7638d5e4 68 dw8XOz65Gg3J2gkmHEHp238keu0 (Fig. S5E) ------- COMMENT: ed6002ed7638d5e4 69 CRvXLy7avsfvmnFqiingXBsHs+E (Fig. S5E, S5F) ------- COMMENT: ed6002ed7638d5e4 70 SeRjS20UIgqJCoVGGb5GUMXIMf0 (Fig. S5F) ------- COMMENT: ed6002ed7638d5e4 71 SeRjS20UIgqJCoVGGb5GUMXIMf0 (Fig. S5F) ------- COMMENT: ed6002ed7638d5e4 72 jTsQneiFN2O87OgZ9cPB1YzCYVU (Fig. S7A) ------- COMMENT: ed6002ed7638d5e4 73 jTsQneiFN2O87OgZ9cPB1YzCYVU (Fig. S7A) ------- COMMENT: ed6002ed7638d5e4 74 KLOzvR5EIb1BMigGZXVDVLWmsW0 (Fig. S7B) ------- COMMENT: ed6002ed7638d5e4 75 KLOzvR5EIb1BMigGZXVDVLWmsW0 (Fig. S7B) ------- COMMENT: ed6002ed7638d5e4 76 5hBvcfhVbME7Bc0BRA9dbyTF2rc (Fig. S7D) ------- COMMENT: ed6002ed7638d5e4 77 5hBvcfhVbME7Bc0BRA9dbyTF2rc (Fig. S7D) ------- COMMENT: ed6002ed7638d5e4 78 prmtlQDYLee+WD05YM58owCnL64 (Fig. S7E) ------- COMMENT: ed6002ed7638d5e4 79 prmtlQDYLee+WD05YM58owCnL64 (Fig. S7E) ------- COMMENT: ed6002ed7638d5e4 80 2fAk/D/9Wy58C+ttX6WQcN/1tjg (Fig. S7G) ------- COMMENT: ed6002ed7638d5e4 81 2fAk/D/9Wy58C+ttX6WQcN/1tjg (Fig. S7G) ------- COMMENT: ed6002ed7638d5e4 82 2fAk/D/9Wy58C+ttX6WQcN/1tjg (Fig. S7G) ------- COMMENT: ed6002ed7638d5e4 83 2fAk/D/9Wy58C+ttX6WQcN/1tjg (Fig. S7G) ------- COMMENT: ed6002ed7638d5e4 84 2fAk/D/9Wy58C+ttX6WQcN/1tjg (Fig. S7G) ------- COMMENT: ed6002ed7638d5e4 85 2fAk/D/9Wy58C+ttX6WQcN/1tjg (Fig. S7G) ------- COMMENT: ed6002ed7638d5e4 86 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: ed6002ed7638d5e4 87 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: ed6002ed7638d5e4 88 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: ed6002ed7638d5e4 89 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: ed6002ed7638d5e4 90 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: ed6002ed7638d5e4 91 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: ed6002ed7638d5e4 92 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: ed6002ed7638d5e4 93 rMuq26pUK0Q0PxRZ520T9hK8hgk (Fig. S3A) ------- COMMENT: ed6002ed7638d5e4 94 na8boxULcmrLrpxyNgxZlFRYT9g (Fig. S3B) ------- COMMENT: ed6002ed7638d5e4 95 na8boxULcmrLrpxyNgxZlFRYT9g (Fig. S3B) ------- COMMENT: ed6002ed7638d5e4 96 LD65OchkY8RFOt3lV85b3I6laDw (Fig. S3C, S3D) ------- COMMENT: ed6002ed7638d5e4 97 LD65OchkY8RFOt3lV85b3I6laDw (Fig. S3C, S3D) ------- COMMENT: ed6002ed7638d5e4 98 LD65OchkY8RFOt3lV85b3I6laDw (Fig. S3C, S3D) ------- COMMENT: ed6002ed7638d5e4 99 RD7246lbLWeEd/53Eqj3QTu+YBk (Fig. S3D) ------- COMMENT: ed6002ed7638d5e4 100 RD7246lbLWeEd/53Eqj3QTu+YBk (Fig. S3D) ------- COMMENT: ed6002ed7638d5e4 101 RD7246lbLWeEd/53Eqj3QTu+YBk (Fig. S3D) ------- COMMENT: ed6002ed7638d5e4 102 RD7246lbLWeEd/53Eqj3QTu+YBk (Fig. S3D) ------- COMMENT: ed6002ed7638d5e4 103 RD7246lbLWeEd/53Eqj3QTu+YBk (Fig. S3D) ------- COMMENT: ed6002ed7638d5e4 104 RD7246lbLWeEd/53Eqj3QTu+YBk (Fig. S3D) ------- COMMENT: ed8fef19dce7d13a 1 YIiduypr245N9E02twiqS8PhqZc fig 1C ------- COMMENT: ed8fef19dce7d13a 2 YIiduypr245N9E02twiqS8PhqZc fig 1C ------- COMMENT: ed8fef19dce7d13a 3 YIiduypr245N9E02twiqS8PhqZc fig 1C ------- COMMENT: ed8fef19dce7d13a 4 YIiduypr245N9E02twiqS8PhqZc fig 1C ------- COMMENT: ed8fef19dce7d13a 5 Ntwrd88Pz+HCZljUiVZgCHLnfq8 fig 1D ------- COMMENT: ed8fef19dce7d13a 6 w4NbdZgeLLDMU5O+vy38ApEkoyI fig 1D, 1E ------- COMMENT: ed8fef19dce7d13a 7 w4NbdZgeLLDMU5O+vy38ApEkoyI fig 1D, 1E ------- COMMENT: ed8fef19dce7d13a 8 w4NbdZgeLLDMU5O+vy38ApEkoyI fig 1D, 1E ------- COMMENT: ed8fef19dce7d13a 12 PCfvBOxnF37/BpYFJ2UuNLcJ1DY Fig 1E ------- COMMENT: ed8fef19dce7d13a 13 PCfvBOxnF37/BpYFJ2UuNLcJ1DY Fig 1E ------- COMMENT: ed8fef19dce7d13a 14 PCfvBOxnF37/BpYFJ2UuNLcJ1DY Fig 1E ------- COMMENT: ed8fef19dce7d13a 15 8K/BTJu00QCy1oOrCEePXxoH318 supplementary material Movies 2-4). ------- COMMENT: ed8fef19dce7d13a 16 8K/BTJu00QCy1oOrCEePXxoH318 supplementary material Movies 2-4). ------- COMMENT: ed8fef19dce7d13a 17 8K/BTJu00QCy1oOrCEePXxoH318 supplementary material Movies 2-4). ------- COMMENT: ed8fef19dce7d13a 18 8K/BTJu00QCy1oOrCEePXxoH318 supplementary material Movies 2-4). ------- COMMENT: ed8fef19dce7d13a 22 2Kje+X+T4+EKkSABoujyyjlabHM fig 2a (comment: this fig also has expression level for mutant alleles) ------- COMMENT: ed8fef19dce7d13a 24 WL4+RrKbFJfBnMGm2BOtMFxtgBw fig 2B, 2C ------- COMMENT: ed8fef19dce7d13a 25 WL4+RrKbFJfBnMGm2BOtMFxtgBw fig 2B, 2C ------- COMMENT: ed8fef19dce7d13a 26 WL4+RrKbFJfBnMGm2BOtMFxtgBw fig 2B, 2C ------- COMMENT: ed8fef19dce7d13a 28 Jss+zFDXX+PhmvvPzdDzJf5Ihek Fig 2D-F ------- COMMENT: ed8fef19dce7d13a 29 Jss+zFDXX+PhmvvPzdDzJf5Ihek Fig 2D-F ------- COMMENT: ed8fef19dce7d13a 30 Jss+zFDXX+PhmvvPzdDzJf5Ihek Fig 2D-F ------- COMMENT: ed8fef19dce7d13a 31 Jss+zFDXX+PhmvvPzdDzJf5Ihek Fig 2D-F ------- COMMENT: ed8fef19dce7d13a 33 Jss+zFDXX+PhmvvPzdDzJf5Ihek Fig 2D-F ------- COMMENT: ed8fef19dce7d13a 34 Jss+zFDXX+PhmvvPzdDzJf5Ihek Fig 2D-F ------- COMMENT: ed8fef19dce7d13a 35 Jss+zFDXX+PhmvvPzdDzJf5Ihek Fig 2D-F ------- COMMENT: ed8fef19dce7d13a 36 Jss+zFDXX+PhmvvPzdDzJf5Ihek Fig 2D-F ------- COMMENT: ed8fef19dce7d13a 37 Jss+zFDXX+PhmvvPzdDzJf5Ihek Fig 2D-F ------- COMMENT: ed8fef19dce7d13a 38 Jss+zFDXX+PhmvvPzdDzJf5Ihek Fig 2D-F ------- COMMENT: ed8fef19dce7d13a 39 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: ed8fef19dce7d13a 44 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: ed8fef19dce7d13a 45 RPdkqAedukJ0cWGJswoHwJ+rPAE fig3 ------- COMMENT: ed8fef19dce7d13a 54 oCiAo1ImcODqcU8FIpJD2j2nIUw Fig4a ------- COMMENT: ed8fef19dce7d13a 55 uNXSAyD4ADIvbjCxowufICyPd0k Fig4c ------- COMMENT: ed8fef19dce7d13a 56 uNXSAyD4ADIvbjCxowufICyPd0k Fig4c ------- COMMENT: ed8fef19dce7d13a 57 Ozp47kvNh5k2BQB6BeY/MCsDsaY Fig4D ------- COMMENT: ed8fef19dce7d13a 58 Ozp47kvNh5k2BQB6BeY/MCsDsaY Fig4D ------- COMMENT: ed8fef19dce7d13a 59 PCfvBOxnF37/BpYFJ2UuNLcJ1DY Fig 1E ------- COMMENT: ed8fef19dce7d13a 62 kor5ydSTLm+79wdjWh2bbBsIaGw supplementary material Fig. S1) ------- COMMENT: ed8fef19dce7d13a 63 UOM3r++xC3o2DJsbseE4OfISEDw fig 4F ------- COMMENT: ed8fef19dce7d13a 64 kor5ydSTLm+79wdjWh2bbBsIaGw supplementary material Fig. S1) ------- COMMENT: ed8fef19dce7d13a 65 UOM3r++xC3o2DJsbseE4OfISEDw fig 4F ------- COMMENT: ed8fef19dce7d13a 71 Ntwrd88Pz+HCZljUiVZgCHLnfq8 fig 1D ------- COMMENT: ed8fef19dce7d13a 72 w4NbdZgeLLDMU5O+vy38ApEkoyI fig 1D, 1E ------- COMMENT: ed8fef19dce7d13a 73 w4NbdZgeLLDMU5O+vy38ApEkoyI fig 1D, 1E ------- COMMENT: ed8fef19dce7d13a 74 SC3RvtG3vOPlHrwdqMZK8e7rcps we conclude that the function of Mto2 in MT nucleation is mediated primarily, if not exclusively, via its binding to Mto1. Moreover, the failure of Mto1-334 to immunoprecipitate the γ-TuC indicates that the Mto1-Mto2 interaction is required for an efficient association of Mto1 with the γ-TuC, as detected in cytoplasmic extracts. ------- COMMENT: ed8fef19dce7d13a 75 SC3RvtG3vOPlHrwdqMZK8e7rcps we conclude that the function of Mto2 in MT nucleation is mediated primarily, if not exclusively, via its binding to Mto1. Moreover, the failure of Mto1-334 to immunoprecipitate the γ-TuC indicates that the Mto1-Mto2 interaction is required for an efficient association of Mto1 with the γ-TuC, as detected in cytoplasmic extracts. ------- COMMENT: ed9270b453b0c024 1 sUCS79Rs9xFD1wViTRlsgeGdNFs (Fig. 6B) ------- COMMENT: ed9270b453b0c024 2 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: ed9270b453b0c024 3 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: ed9270b453b0c024 4 D16xxbzPnKrHvhzRsR0J2Y8IgQg (Fig. 4C, Fig. 6C) ------- COMMENT: ed9270b453b0c024 5 3tiRvvXHb4m6hKPecIoopNwy4s8 (Fig. 2A, Fig. 4A) ------- COMMENT: ed9270b453b0c024 6 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: ed9270b453b0c024 7 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: ed9270b453b0c024 8 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: ed9270b453b0c024 9 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: ed9270b453b0c024 10 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: ed9270b453b0c024 11 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: ed9270b453b0c024 12 0vX3QoEJE2yeLmXoOtcrRTP1Mfs (Fig. 2B) ------- COMMENT: ed9270b453b0c024 13 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: ed9270b453b0c024 14 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: ed9270b453b0c024 15 2htLY2idoP7cVXHfx1F8zpnpv6U (Fig. 2D) ------- COMMENT: ed9270b453b0c024 16 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: ed9270b453b0c024 17 GkI93EYSqP6NybAtsJjxO2fy3Ic (Fig. 4D) ------- COMMENT: ed9270b453b0c024 18 MfhXrratyk9HEpPvYNKyPqJLEoI (Fig. 4E) ------- COMMENT: ed9270b453b0c024 19 MfhXrratyk9HEpPvYNKyPqJLEoI (Fig. 4E) ------- COMMENT: ed9270b453b0c024 20 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: ed9270b453b0c024 21 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: ed9270b453b0c024 22 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: ed9270b453b0c024 23 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: ed9270b453b0c024 24 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: ed9270b453b0c024 25 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: ed9270b453b0c024 26 sUCS79Rs9xFD1wViTRlsgeGdNFs (Fig. 6B) ------- COMMENT: ed9270b453b0c024 27 sUCS79Rs9xFD1wViTRlsgeGdNFs (Fig. 6B) ------- COMMENT: ed9270b453b0c024 28 sUCS79Rs9xFD1wViTRlsgeGdNFs (Fig. 6B) ------- COMMENT: ed9270b453b0c024 29 sUCS79Rs9xFD1wViTRlsgeGdNFs (Fig. 6B) ------- COMMENT: ed9270b453b0c024 30 sUCS79Rs9xFD1wViTRlsgeGdNFs (Fig. 6B) ------- COMMENT: ed9270b453b0c024 31 wnx2gS3IAKqzPkStlyJ0uF9GveM (Fig. 6C) ------- COMMENT: ed9270b453b0c024 32 wnx2gS3IAKqzPkStlyJ0uF9GveM (Fig. 6C) ------- COMMENT: ed9270b453b0c024 33 wnx2gS3IAKqzPkStlyJ0uF9GveM (Fig. 6C) ------- COMMENT: ed9270b453b0c024 34 wnx2gS3IAKqzPkStlyJ0uF9GveM (Fig. 6C) ------- COMMENT: ed9270b453b0c024 35 wnx2gS3IAKqzPkStlyJ0uF9GveM (Fig. 6C) ------- COMMENT: ed9270b453b0c024 36 9waAVtCUtZq5Uk7br6o7D9rwUB0 We propose that the resultant chromatin organization is at least in part responsible for the altered donor choices in HULC and Set1C mutants (Fig. 7). ------- COMMENT: ed9270b453b0c024 37 9waAVtCUtZq5Uk7br6o7D9rwUB0 We propose that the resultant chromatin organization is at least in part responsible for the altered donor choices in HULC and Set1C mutants (Fig. 7). ------- COMMENT: ed9270b453b0c024 38 9waAVtCUtZq5Uk7br6o7D9rwUB0 We propose that the resultant chromatin organization is at least in part responsible for the altered donor choices in HULC and Set1C mutants (Fig. 7). ------- COMMENT: ed9270b453b0c024 39 9waAVtCUtZq5Uk7br6o7D9rwUB0 We propose that the resultant chromatin organization is at least in part responsible for the altered donor choices in HULC and Set1C mutants (Fig. 7). ------- COMMENT: ed9270b453b0c024 40 9waAVtCUtZq5Uk7br6o7D9rwUB0 We propose that the resultant chromatin organization is at least in part responsible for the altered donor choices in HULC and Set1C mutants (Fig. 7). ------- COMMENT: ed9652a888c24c94 1 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: ed9652a888c24c94 5 1R/coDzLXAp51uw0VT0Kwdfjqcg Even reduction to about 10% of the original Mad1 level, which is hardly visible by fluorescence microscopy (Fig. 2e), did not fully abolish the SAC ------- COMMENT: ed9652a888c24c94 6 Teh/6sIIDzpifGT7xBJfTVFqO1k Fig. 5a. ------- COMMENT: ed9652a888c24c94 7 FbN4Q0rmIqqEnaMI1EwExpQp/dw (comment: CHECK abundances of 40% or lower), cells lacked checkpoint activity. ------- COMMENT: ed9652a888c24c94 9 pZAHxbp9YLmjh5NWTWZt/NPnaBM (Fig. 2d) ------- COMMENT: ed9652a888c24c94 19 +3ylHQi+fMEWzwuYGkJVfM5hazc In cells with 30% Mad1, the checkpoint was markedly impaired in minimal medium, although largely functional in rich medium ------- COMMENT: ed9652a888c24c94 21 jJka65ROGh/Htbh4f/yqtcdK7+s (comment: 300% of endogenous mad1 level), Fig. S4 ------- COMMENT: ed9652a888c24c94 22 VV7cY2jpCzcqbn81jkxO7NN/tM8 (comment: 10% of endogenous mad1 level), Fig. S4 ------- COMMENT: ed9652a888c24c94 23 4kAUQl4GzAkvGpDbUsBxmN+n8cg (comment: 30% of endogenous mad1 level), Fig. S4 ------- COMMENT: ed9652a888c24c94 24 hUvlDqKhF2mppGAgjWxZ0CMj1as (comment: 10% of endogenous mad2 level), Fig. S4 ------- COMMENT: ed9652a888c24c94 25 kONp3n19ET/MBK3dljgh8FTuaXU (comment: 65% of endogenous mad2 level), Fig. S4 ------- COMMENT: ed9652a888c24c94 26 QyWDXBPqnwd5VaQYXuVynUgXlmo (comment: 30% of endogenous mad3 level), Fig. S4 ------- COMMENT: ed9652a888c24c94 27 YdJYhQytmShzCWZ7Go6m9pJexXo (comment: 40% of endogenous slp1 level), Fig. S4 ------- COMMENT: ed9652a888c24c94 28 B0R6wmA20NNAqxdO/vuRvia1pgo (comment: 500% of endogenous mad1 level), Fig. S4 ------- COMMENT: ed99f40936cc1718 1 X+f7jOdkqktZ6PIXEeEHawqZdAQ (comment: CHECK during stationary phase). Figure 6C In fact, the protein levels of Scw1 markedly decreased in ageing cells. ------- COMMENT: ed99f40936cc1718 3 vCV1RbTEi0aqCHHqDmTlfjuMKXY Figure 4B Accordingly, our smFISH experiment showed that tlh2 was de-repressed in sir2 deletion cells ------- COMMENT: ed99f40936cc1718 4 TWrhVwX9jB9KoD56bkh2VInSUwc Figure 5A Cells lacking Sir2 showed a subtle extension of chronological lifespan compared to wild-type, especially at later timepoints ------- COMMENT: ed99f40936cc1718 5 75zzvCkgOnZ9Red64IWKg5k/zyc Based on DNA breakpoint junctions in genome sequence data (Fig 5B While wild-type cells showed a substantial increase of indels in aged cells, sir2Δ cells showed only a subtle increase ------- COMMENT: ed99f40936cc1718 6 9YjGKQta6+vYEZXjp9rMxfezwP4 Figure S7C Moreover, the tlh2 overexpression strain was substantially shorter-lived than wild-type cells ------- COMMENT: ed99f40936cc1718 7 dARcGBfkLt4gDxqaib8LSAWZryI Fig. 5B Notably, in aged cells the double mutant showed an ~3-fold increase in chro- mosomal junctions on average, albeit with large variation, but no increase in indels ------- COMMENT: ed99f40936cc1718 8 rCMGlUFfrZOpYlfFJ2yCFaNG/m4 Fig 5A Given the increased lifespan of rnh1Δ rnh201Δ cells ------- COMMENT: ed99f40936cc1718 9 ZOPOpu7jWBOkZGeZ+kCwe92vvkw Fig. S7A, Fig A re-analysis of RNA-seq data from non-dividing cells [68] revealed a subtle increase in tlh2 expression during chronological ageing ------- COMMENT: ed99f40936cc1718 10 aERQJWTkD5UC56A+3wva9E5ZNDg Figure S7B The proportion of junctions down- stream of tlh2 was higher in the tlh2 overexpression strain compared to wild-type ------- COMMENT: eda37cd3f3ffb2ce 20 fpOwJWwy+bhL8MgfuRbITkUqMCU (comment: IMP evidence for part_of extension) ------- COMMENT: eda37cd3f3ffb2ce 21 fpOwJWwy+bhL8MgfuRbITkUqMCU (comment: IMP evidence for part_of extension) ------- COMMENT: edf1fcf16969393b 1 Kun5fXey03yMIVfjtIa3KYYJgnE (comment: ch16) ------- COMMENT: edf9edf5b9dffcea 37 V26Y5WzF5aOB2xBbSlfPBSOWY/U (comment: not shown direct binding but want to capture the fact that it binds the oxidised form) ------- COMMENT: edf9edf5b9dffcea 50 V26Y5WzF5aOB2xBbSlfPBSOWY/U (comment: not shown direct binding but want to capture the fact that it binds the oxidised form) ------- COMMENT: edfddf218bf755ae 25 JXL+25YGUGFYNewhab07DkrWca4 "These results indicate that Mal3 is required for the proper association of Tea2 with microtubules and sug- gest that Mal3 stabilizes the kinesin–microtubule interaction" ------- COMMENT: edfddf218bf755ae 27 n5W+Nwm2kcSiRsrVw4kdaImZGtg (comment: LOCALIZES OK, IS NOT RETAINED) ------- COMMENT: edfee9c20ff8ca78 1 gm1Px94b5UBdjxqoHSq63NZdxOQ table1 ------- COMMENT: edfee9c20ff8ca78 27 l165mgamoT9Y90wthcjZQM/bn5E table 1 ------- COMMENT: edfee9c20ff8ca78 28 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: edfee9c20ff8ca78 29 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: edfee9c20ff8ca78 30 uOitulEI8+um1PohhrtyVNqIp/o ue to their antagonistic roles in cohesion establish- ment, the lethality of eso1D can be suppressed by deletion of wpl1 (Feytout et al. 2011; Kagami et al. 2011). Whereas wpl1D did not show defects in heterochromatic silencing, the eso1D wpl1D double mutant showed derepression of mat2P::ura4+ and haploid meiosis similar to pds5D cells (Figure 6A) ------- COMMENT: edfee9c20ff8ca78 31 5Leuj0nQtQ/oH58v8XLkYXSDMog Figure 6B ------- COMMENT: edfee9c20ff8ca78 38 sFzW9qm7niOZlyN47T2ePHtAW7I fig 2B (Figure S1B and Table S2). This variegated staining pattern is a char- acteristic of mutants that are known to be defective in the main- tenance of heterochromatin and that show a reduction, but not loss, of H3K9me levels (Taneja et al. 2017). Indeed, ChIP anal- yses of H3K9 di- and trimethylation (H3K9me2/3) showed a reduction in heterochromatic H3K9 marks at or near mat2P in pds5D (Figure 2B). ------- COMMENT: edfee9c20ff8ca78 42 bUA/agW+Xd8WyyODrmcX5T7+lWA Fig 5B (comment: vw: moved pds5 to assayed target) ------- COMMENT: edfee9c20ff8ca78 45 v1uabuLlf/voX0ZycUM0lzta+CY Figure 6A) ------- COMMENT: edfee9c20ff8ca78 46 /vfA9gYsyByV1dB1PqfZChE4N1g fig1d ------- COMMENT: edfee9c20ff8ca78 48 D97mpWRHCsXfFAVVKetlM6NxwBk fig 1D, 1E ------- COMMENT: edfee9c20ff8ca78 49 D97mpWRHCsXfFAVVKetlM6NxwBk fig 1D, 1E ------- COMMENT: edfee9c20ff8ca78 50 D97mpWRHCsXfFAVVKetlM6NxwBk fig 1D, 1E ------- COMMENT: edfee9c20ff8ca78 52 nrktwcJ4c/K1ADCXY2DgLzuxHhA cells revealed that H3K9me2 was notably decreased at cen- tromeres and telomeres in pds5D (Figure 3, A and B). ------- COMMENT: edfee9c20ff8ca78 53 nrktwcJ4c/K1ADCXY2DgLzuxHhA cells revealed that H3K9me2 was notably decreased at cen- tromeres and telomeres in pds5D (Figure 3, A and B). ------- COMMENT: edfee9c20ff8ca78 54 cXozfjzPCZxP6mrMLNbbvryfPsk Figure 4A (comment: CHECK https://github.com/pombase/fypo/issues/3693) ------- COMMENT: edfee9c20ff8ca78 55 nV82mGN1Q0BU12Ze6WPy5IV09Tg Compared to the single ago1D or pds5D deletion mutants, the ago1D pds5D double mutant showed severe loss-of-silencing of Kint2::ura4+ (Figure S2C). ------- COMMENT: edfee9c20ff8ca78 61 gakABnhGtEpGG38rYO6I8hXXxds However, the localization of Pds5 to euchromatic locations was unaffected in hetero- chromatin-deficient cells (Figure 5E) ------- COMMENT: edfee9c20ff8ca78 62 gakABnhGtEpGG38rYO6I8hXXxds However, the localization of Pds5 to euchromatic locations was unaffected in hetero- chromatin-deficient cells (Figure 5E) ------- COMMENT: edfee9c20ff8ca78 63 uOitulEI8+um1PohhrtyVNqIp/o ue to their antagonistic roles in cohesion establish- ment, the lethality of eso1D can be suppressed by deletion of wpl1 (Feytout et al. 2011; Kagami et al. 2011). Whereas wpl1D did not show defects in heterochromatic silencing, the eso1D wpl1D double mutant showed derepression of mat2P::ura4+ and haploid meiosis similar to pds5D cells (Figure 6A) ------- COMMENT: edfee9c20ff8ca78 64 pXrlKP2lSdjQyoQpZWuV54pJHrI Figure S5 Moreover, when we deleted pds5 in cells lacking Eso1 and/or Wpl1, the levels of haploid meiosis displayed by double or triple mutants were comparable to that of single-mutant pds5D ------- COMMENT: edfee9c20ff8ca78 65 sRbiU/jtYRJxVkHoyWOhqsXRQaU Figure S5 ------- COMMENT: edfee9c20ff8ca78 66 5Leuj0nQtQ/oH58v8XLkYXSDMog Figure 6B ------- COMMENT: edfee9c20ff8ca78 67 5Leuj0nQtQ/oH58v8XLkYXSDMog Figure 6B ------- COMMENT: ee41f2de8b73ee9b 1 7hjEziSY661O7fW4WREktMiJo8Q Our fluorescence microscopic analysis also clearly indicated that a significant percentage of the hst4 cells in culture had fragmented DNA (see Fig. S1 in the sup- plemental material). ------- COMMENT: ee41f2de8b73ee9b 2 vjaeKgG8ohKjQJ82NCeBmKtq5NE Interestingly, hst4 cells were sensitive to MMS and CPT (Fig. 1A) ------- COMMENT: ee41f2de8b73ee9b 3 vjaeKgG8ohKjQJ82NCeBmKtq5NE Interestingly, hst4 cells were sensitive to MMS and CPT (Fig. 1A) ------- COMMENT: ee41f2de8b73ee9b 4 74mXpi56G+Y5Jih023+CNLTn3qw (comment: CHECK significantly sensitive to HU.) ------- COMMENT: ee41f2de8b73ee9b 5 wMK+ZYhb5k71ZJ+q1n4syG4CJaQ (comment: CHECK significantly sensitive to HU. less than that of wild-type cells but higher than that of checkpoint mutant rad3 cells) (Fig. 1B). ------- COMMENT: ee41f2de8b73ee9b 6 hRPhjpVez1hTvW42BmGhAp90sS4 hst4 cells behaved as wild-type cells did and were able to survive exposure to gamma irradiation (Fig. 1C). ------- COMMENT: ee41f2de8b73ee9b 7 LqZVHSBcnXDUf1LItRF0+Z8nAQE (comment: If this signal is not generated, cells mutant go through the cell cycle with damage and eventually die.) ------- COMMENT: ee41f2de8b73ee9b 8 u7tKvH9Wc1fw016Pvn169UWQivM Tetrad analysis demonstrating synthetic lethality of representatives of the DNA damage checkpoint mutant crb2 and rad3 are shown in Fig. 2A ------- COMMENT: ee41f2de8b73ee9b 9 u7tKvH9Wc1fw016Pvn169UWQivM Tetrad analysis demonstrating synthetic lethality of representatives of the DNA damage checkpoint mutant crb2 and rad3 are shown in Fig. 2A ------- COMMENT: ee41f2de8b73ee9b 10 u7tKvH9Wc1fw016Pvn169UWQivM Tetrad analysis demonstrating synthetic lethality of representatives of the DNA damage checkpoint mutant crb2 and rad3 are shown in Fig. 2A ------- COMMENT: ee41f2de8b73ee9b 11 u7tKvH9Wc1fw016Pvn169UWQivM Tetrad analysis demonstrating synthetic lethality of representatives of the DNA damage checkpoint mutant crb2 and rad3 are shown in Fig. 2A ------- COMMENT: ee41f2de8b73ee9b 12 mo4qDtbZUWT/Eh9Hx1ya071/esc In cells lacking Hst4, Chk1 was phosphorylated even in the absence of MMS exposure, and this phosphorylation did not significantly in- crease upon exposure to MMS (Fig. 2B). ------- COMMENT: ee41f2de8b73ee9b 13 j7w2jHQz482dbmhP+M51zRqEnTQ Wild-type cells showed a peak of septation approximately 80 min after release, whereas the hst4 cells showed a delayed peak of septation at 120 min (see Fig. S2 in the supplemental material). ------- COMMENT: ee41f2de8b73ee9b 14 u7tKvH9Wc1fw016Pvn169UWQivM Tetrad analysis demonstrating synthetic lethality of representatives of the DNA damage checkpoint mutant crb2 and rad3 are shown in Fig. 2A ------- COMMENT: ee41f2de8b73ee9b 15 u7tKvH9Wc1fw016Pvn169UWQivM Tetrad analysis demonstrating synthetic lethality of representatives of the DNA damage checkpoint mutant crb2 and rad3 are shown in Fig. 2A ------- COMMENT: ee41f2de8b73ee9b 16 u7tKvH9Wc1fw016Pvn169UWQivM Tetrad analysis demonstrating synthetic lethality of representatives of the DNA damage checkpoint mutant crb2 and rad3 are shown in Fig. 2A ------- COMMENT: ee41f2de8b73ee9b 17 u7tKvH9Wc1fw016Pvn169UWQivM Tetrad analysis demonstrating synthetic lethality of representatives of the DNA damage checkpoint mutant crb2 and rad3 are shown in Fig. 2A ------- COMMENT: ee41f2de8b73ee9b 19 u7tKvH9Wc1fw016Pvn169UWQivM Tetrad analysis demonstrating synthetic lethality of representatives of the DNA damage checkpoint mutant crb2 and rad3 are shown in Fig. 2A ------- COMMENT: ee41f2de8b73ee9b 20 LHQRYoUAUUVehLXdwdo72Pzc3Tk However, every single repair mutant that we tested was synthetically sick in combination with hst4 when cells were grown on medium containing MMS (Fig. 3). ------- COMMENT: ee41f2de8b73ee9b 21 LHQRYoUAUUVehLXdwdo72Pzc3Tk However, every single repair mutant that we tested was synthetically sick in combination with hst4 when cells were grown on medium containing MMS (Fig. 3). ------- COMMENT: ee41f2de8b73ee9b 22 LHQRYoUAUUVehLXdwdo72Pzc3Tk However, every single repair mutant that we tested was synthetically sick in combination with hst4 when cells were grown on medium containing MMS (Fig. 3). ------- COMMENT: ee41f2de8b73ee9b 23 LHQRYoUAUUVehLXdwdo72Pzc3Tk However, every single repair mutant that we tested was synthetically sick in combination with hst4 when cells were grown on medium containing MMS (Fig. 3). ------- COMMENT: ee41f2de8b73ee9b 24 LHQRYoUAUUVehLXdwdo72Pzc3Tk However, every single repair mutant that we tested was synthetically sick in combination with hst4 when cells were grown on medium containing MMS (Fig. 3). ------- COMMENT: ee41f2de8b73ee9b 25 0aitUhng08ADCBXGD1AtZ3NV3c4 The hst4 mutant did not show significant changes in the acetylation levels of histone H3 K9 or K14 and histone H4 K16 compared to the wild-type control (Fig. 4A). ------- COMMENT: ee41f2de8b73ee9b 26 0aitUhng08ADCBXGD1AtZ3NV3c4 The hst4 mutant did not show significant changes in the acetylation levels of histone H3 K9 or K14 and histone H4 K16 compared to the wild-type control (Fig. 4A). ------- COMMENT: ee41f2de8b73ee9b 27 0aitUhng08ADCBXGD1AtZ3NV3c4 The hst4 mutant did not show significant changes in the acetylation levels of histone H3 K9 or K14 and histone H4 K16 compared to the wild-type control (Fig. 4A). ------- COMMENT: ee41f2de8b73ee9b 28 pOU3Mr1FqkIFuXLNJzFBZxqPEDA In the absence of Sir2, we observed elevated levels of histone H3 K9 and histone H4 K16, which was con- sistent with previous reports (17, 48). ------- COMMENT: ee41f2de8b73ee9b 29 pOU3Mr1FqkIFuXLNJzFBZxqPEDA In the absence of Sir2, we observed elevated levels of histone H3 K9 and histone H4 K16, which was con- sistent with previous reports (17, 48). ------- COMMENT: ee41f2de8b73ee9b 30 s2y2Lap33qt/ibM1wlMjCMZ+n7c These results (Fig. 4C) showed that the acetylation of H3 K56 is cell cycle regulated and occurred during the S phase of the cell cycle. ------- COMMENT: ee41f2de8b73ee9b 31 vVhq3vSPt602o3SmtRenygFow08 H184Y mutation were as sensitive to MMS as the hst4 strains were, indicating that Hst4 enzymatic activity was important for cells to be resistant to MMS. ------- COMMENT: ee41f2de8b73ee9b 32 QsXejAryW1V4VIQeVmG3f20aS2Y These results collectively suggest that alterations in K56 acetylation are dependent upon the presence of Hst4 in the cell, and more importantly, they suggest that the levels of Hst4 are regulated in response to cell cycle progression and DNA damage. ------- COMMENT: ee41f2de8b73ee9b 33 c+15vA6ghf4o1KydXABc8tdBq84 Both mutant strains were viable and able to grow at 32°C. However, like the hst4 strain, the histone H3 K56R mutant strains had slight growth defects compared to the strain containing a single copy of the H3 histone (Fig. 7B, first panel). ------- COMMENT: ee41f2de8b73ee9b 34 1pQIpo8eRfoXj4TpjL5PeJqFXck Both mutant strains were viable and able to grow at 32°C. ------- COMMENT: ee41f2de8b73ee9b 35 YRmBT27D70NwqQL+zxC00woeZlg Like hst4 cells, both histone H3 mutant cells were very elongated, and a percentage of these cells exhibited abnormal DAPI staining (Fig. 7A). ------- COMMENT: ee41f2de8b73ee9b 36 YRmBT27D70NwqQL+zxC00woeZlg Like hst4 cells, both histone H3 mutant cells were very elongated, and a percentage of these cells exhibited abnormal DAPI staining (Fig. 7A). ------- COMMENT: ee41f2de8b73ee9b 37 YRmBT27D70NwqQL+zxC00woeZlg Like hst4 cells, both histone H3 mutant cells were very elongated, and a percentage of these cells exhibited abnormal DAPI staining (Fig. 7A). ------- COMMENT: ee41f2de8b73ee9b 38 xE/aECUhf7VrLN3K7ihcipoJ+ss Interestingly, similar to the hst4 mutant, both K56R and K56Q mutants were sensitive to MMS and CPT. ------- COMMENT: ee41f2de8b73ee9b 39 Ck9uPkZqkQ88C/sAAOP5lg9mlBs Interestingly, similar to the hst4 mutant, both K56R and K56Q mutants were sensitive to MMS and CPT. However, the histone H3 K56Q mutant, which mimics the constitutively acetylated state, was less sensitive to MMS and CPT than the K56R mutant, which mimics the constitutively deacetylated state. ------- COMMENT: ee55ca52710667a5 3 XSenlmud2GKYpxO0ZLf3xCzfYHo (comment: CHECK regulated by inositol) ------- COMMENT: ee55ca52710667a5 4 XSenlmud2GKYpxO0ZLf3xCzfYHo (comment: CHECK regulated by inositol) ------- COMMENT: ee55ca52710667a5 5 ezwF1k40ki07np1xdQ4EyuQTMcI (comment: CHECK GO:0008444 CDP-DG synthase and GO:0003882 PS synthase) ------- COMMENT: ee65028085190b26 1 T+luLtKkbsXkrYxU8Bo2dfFtgGU Fig. S1A ------- COMMENT: ee65028085190b26 2 T+luLtKkbsXkrYxU8Bo2dfFtgGU Fig. S1A ------- COMMENT: ee65028085190b26 3 T+luLtKkbsXkrYxU8Bo2dfFtgGU Fig. S1A ------- COMMENT: ee65028085190b26 4 T+luLtKkbsXkrYxU8Bo2dfFtgGU Fig. S1A ------- COMMENT: ee65028085190b26 5 T+luLtKkbsXkrYxU8Bo2dfFtgGU Fig. S1A ------- COMMENT: ee65028085190b26 6 T+luLtKkbsXkrYxU8Bo2dfFtgGU Fig. S1A ------- COMMENT: ee65028085190b26 7 T+luLtKkbsXkrYxU8Bo2dfFtgGU Fig. S1A ------- COMMENT: ee65028085190b26 8 T+luLtKkbsXkrYxU8Bo2dfFtgGU Fig. S1A ------- COMMENT: ee65028085190b26 9 T+luLtKkbsXkrYxU8Bo2dfFtgGU Fig. S1A ------- COMMENT: ee65028085190b26 10 T+luLtKkbsXkrYxU8Bo2dfFtgGU Fig. S1A ------- COMMENT: ee65028085190b26 11 T+luLtKkbsXkrYxU8Bo2dfFtgGU Fig. S1A ------- COMMENT: ee65028085190b26 12 T+luLtKkbsXkrYxU8Bo2dfFtgGU Fig. S1A ------- COMMENT: ee65028085190b26 13 T+luLtKkbsXkrYxU8Bo2dfFtgGU Fig. S1A ------- COMMENT: ee65028085190b26 14 T+luLtKkbsXkrYxU8Bo2dfFtgGU Fig. S1A ------- COMMENT: ee65028085190b26 15 T+luLtKkbsXkrYxU8Bo2dfFtgGU Fig. S1A ------- COMMENT: ee65028085190b26 16 T+luLtKkbsXkrYxU8Bo2dfFtgGU Fig. S1A ------- COMMENT: ee65028085190b26 41 3MGpBzazGWRUClRNf5aQRVjLth4 affected chromatin association of Swr1, distribution of H2A.Z across the genome (Supplementary Fig. 2c). ------- COMMENT: ee65028085190b26 42 UaOXAux7bYrhNcpF21Bj4buw+e8 Dpht1 causes a slight increase in silencing at the pericentromeric region, but H3K9me distribution at heterochromatic loci is not severely altered (Supplementary Fig. 4a). ------- COMMENT: ee65028085190b26 43 0AH/zW7d6K1BzQGyr0d+7XVB9m8 At euchromatic loci, H2A.Z localizes preferentially in intergenic regions (Supplementary Fig. 3b) ------- COMMENT: ee65028085190b26 44 OHBWysvPCDsaK6B+DrHCYuPzOWQ (Delta)pht1 caused a disproportionate increase in antisense transcripts at many (,5–8%) euchromatic loci (Fig. 1e–g and Supplementary Fig. 5), as confirmed by PCR with strand-specific reverse transcrip- tion (RT–PCR; Fig. 1f). ------- COMMENT: ee65028085190b26 45 ep2UHBvepVJQAKZzJI5xLKoVWUA When Dpht1 was combined with mutant alleles of clr4 or rik1—components of the Clr4-containing methyltransferase complex (ClrC)7—the resultant double mutants showed severe growth defects and a large, synergistic increase in antisense RNAs at .20% of genes (Fig. 2a and Supplementary Figs 5 and 7a, b). Consistent with ClrC directly participating in antisense suppression, Rik1 was found at the convergent loci (Supplementary Fig. 7c). ------- COMMENT: ee65028085190b26 47 ep2UHBvepVJQAKZzJI5xLKoVWUA When Dpht1 was combined with mutant alleles of clr4 or rik1—components of the Clr4-containing methyltransferase complex (ClrC)7—the resultant double mutants showed severe growth defects and a large, synergistic increase in antisense RNAs at .20% of genes (Fig. 2a and Supplementary Figs 5 and 7a, b). Consistent with ClrC directly participating in antisense suppression, Rik1 was found at the convergent loci (Supplementary Fig. 7c). ------- COMMENT: ee65028085190b26 48 ep2UHBvepVJQAKZzJI5xLKoVWUA When Dpht1 was combined with mutant alleles of clr4 or rik1—components of the Clr4-containing methyltransferase complex (ClrC)7—the resultant double mutants showed severe growth defects and a large, synergistic increase in antisense RNAs at .20% of genes (Fig. 2a and Supplementary Figs 5 and 7a, b). Consistent with ClrC directly participating in antisense suppression, Rik1 was found at the convergent loci (Supplementary Fig. 7c). ------- COMMENT: ee65028085190b26 49 ep2UHBvepVJQAKZzJI5xLKoVWUA When Dpht1 was combined with mutant alleles of clr4 or rik1—components of the Clr4-containing methyltransferase complex (ClrC)7—the resultant double mutants showed severe growth defects and a large, synergistic increase in antisense RNAs at .20% of genes (Fig. 2a and Supplementary Figs 5 and 7a, b). Consistent with ClrC directly participating in antisense suppression, Rik1 was found at the convergent loci (Supplementary Fig. 7c). ------- COMMENT: ee65028085190b26 50 ep2UHBvepVJQAKZzJI5xLKoVWUA When Dpht1 was combined with mutant alleles of clr4 or rik1—components of the Clr4-containing methyltransferase complex (ClrC)7—the resultant double mutants showed severe growth defects and a large, synergistic increase in antisense RNAs at .20% of genes (Fig. 2a and Supplementary Figs 5 and 7a, b). Consistent with ClrC directly participating in antisense suppression, Rik1 was found at the convergent loci (Supplementary Fig. 7c). ------- COMMENT: ee65028085190b26 51 ep2UHBvepVJQAKZzJI5xLKoVWUA When Dpht1 was combined with mutant alleles of clr4 or rik1—components of the Clr4-containing methyltransferase complex (ClrC)7—the resultant double mutants showed severe growth defects and a large, synergistic increase in antisense RNAs at .20% of genes (Fig. 2a and Supplementary Figs 5 and 7a, b). Consistent with ClrC directly participating in antisense suppression, Rik1 was found at the convergent loci (Supplementary Fig. 7c). ------- COMMENT: ee65028085190b26 52 MjEuI0faf6sjPtO2bsQQedOnt2E Combining Dago1 with Dpht1 resulted in synergistic upregulation of antisense transcripts, as in Dclr4 Dpht1 (Fig. 2a). When Dpht1 was combined with mutant alleles of clr4 or rik1—components of the Clr4-containing methyltransferase complex (ClrC)7—the resultant double mutants showed severe growth defects and a large, synergistic increase in antisense RNAs at .20% of genes (Fig. 2a and Supplementary Figs 5 and 7a, b). Consistent with ClrC directly participating in antisense suppression, Rik1 was found at the convergent loci (Supplementary Fig. 7c). ------- COMMENT: ee65028085190b26 53 3fqHxUGdL0yoaCXXMQQ9HrhmvLc the synergistic increase in antisense RNAs observed in the Dclr4 Dpht1 mutant was not observed in the Dswi6 Dpht1 mutant (Fig. 2a). ------- COMMENT: ee65028085190b26 54 QoiIugYWsbVit9SRsITg9o5YHPc Deletion of exosome subunit rrp6 led to an antisense profile closely resembling that of Dclr4 Dpht1, with read- through antisense RNA covering entire ORFs at convergent genes (Fig. 3b). When Dpht1 was combined with mutant alleles of clr4 or rik1—components of the Clr4-containing methyltransferase complex (ClrC)7—the resultant double mutants showed severe growth defects and a large, synergistic increase in antisense RNAs at .20% of genes (Fig. 2a and Supplementary Figs 5 and 7a, b). Consistent with ClrC directly participating in antisense suppression, Rik1 was found at the convergent loci (Supplementary Fig. 7c). ------- COMMENT: ee65028085190b26 55 UXdWDzLyo66D94+QKffzugyoTy0 However, antisense RNAs did not accumulate extensively in Dswi6 cells and the synergistic increase in antisense RNAs observed in the Dclr4 Dpht1 mutant was not observed in the Dswi6 Dpht1 mutant (Fig. 2a). Thus, ClrC and Ago1 contribute to antisense suppression by a new mechanism(s). ------- COMMENT: ee707aabec0f40b7 1 25mKmjLWZ4u4siQTDMcuHF+VB6g (comment: This was really IGI complemetnation of E-coli pyrB) ------- COMMENT: eec0954c4a56c293 1 CRnKzVDtePGvY/xVihpS/WBFuEE reproducible decrease in modification at several other sites, most notably A64 (Figure 1C, D, Sup- plementary Figure S3). ------- COMMENT: eec0954c4a56c293 2 CRnKzVDtePGvY/xVihpS/WBFuEE reproducible decrease in modification at several other sites, most notably A64 (Figure 1C, D, Sup- plementary Figure S3). ------- COMMENT: eec0954c4a56c293 3 CRnKzVDtePGvY/xVihpS/WBFuEE reproducible decrease in modification at several other sites, most notably A64 (Figure 1C, D, Sup- plementary Figure S3). ------- COMMENT: eec0954c4a56c293 4 T9jflvHDiJwFFze2OnsyOOmbrko Deletion of snoZ30 and sno530 resulted in a loss of 2′-O-methylation at A41 and A64, respectively, suggesting that sno530 is indeed the A64 U6-modifying snoRNA (Figure 1C, D, Supplementary Figure S3). ------- COMMENT: eec0954c4a56c293 5 Pq38kSYVlOOsTXfkD3eT94zsnio Still, as mean intron retention values indeed showed an in- crease upon Bmc1 deletion (Figure 5A), we chose several representative intron retention events to validate with semi- quantitative RT-PCR (one of which, intron 1 of pud1, dis- played a statistically significant increase upon Bmc1 dele- tion at 32 ̊C in our RNA Seq dataset). ------- COMMENT: eec0954c4a56c293 6 T9jflvHDiJwFFze2OnsyOOmbrko Deletion of snoZ30 and sno530 resulted in a loss of 2′-O-methylation at A41 and A64, respectively, suggesting that sno530 is indeed the A64 U6-modifying snoRNA (Figure 1C, D, Supplementary Figure S3). ------- COMMENT: eec0954c4a56c293 7 T9jflvHDiJwFFze2OnsyOOmbrko Deletion of snoZ30 and sno530 resulted in a loss of 2′-O-methylation at A41 and A64, respectively, suggesting that sno530 is indeed the A64 U6-modifying snoRNA (Figure 1C, D, Supplementary Figure S3). ------- COMMENT: eec0954c4a56c293 11 MZdQD/dBPimW2Mu3tJHFsPTeY9g we found that all three proteins are neces- sary for an interaction with U6 (Figure 1A, Supplemen- tary Figure S1B). ------- COMMENT: eec0954c4a56c293 12 MZdQD/dBPimW2Mu3tJHFsPTeY9g we found that all three proteins are neces- sary for an interaction with U6 (Figure 1A, Supplemen- tary Figure S1B). ------- COMMENT: eec0954c4a56c293 13 MZdQD/dBPimW2Mu3tJHFsPTeY9g we found that all three proteins are neces- sary for an interaction with U6 (Figure 1A, Supplemen- tary Figure S1B). ------- COMMENT: eec0954c4a56c293 14 VmE8VOz/FSM9AEDdSFYL8PIQmwk Importantly, co-migration of Pof8 with U6 was lost upon deletion of Bmc1 (Figure 1B), as well as co-migration of Bmc1 with U6 upon deletion of Pof8 (Supplementary Fig- ure S1C). ------- COMMENT: eec0954c4a56c293 15 VmE8VOz/FSM9AEDdSFYL8PIQmwk Importantly, co-migration of Pof8 with U6 was lost upon deletion of Bmc1 (Figure 1B), as well as co-migration of Bmc1 with U6 upon deletion of Pof8 (Supplementary Fig- ure S1C). ------- COMMENT: eec0954c4a56c293 16 NjHJrUgueOkbvEzkbfkWEYWI/SA we examined our Bmc1 RIP-Seq dataset (20), which revealed an interaction between Bmc1 and snoZ30, which guides 2′-O-methylation of U6 at position 41 (41) (Supplementary Figures S1A, S2A, B). ------- COMMENT: eec0954c4a56c293 17 GxfJNMbwdf7V+jy6QqjocjpSUl8 Further support- ing the idea that U6 complex formation is contingent on the presence of all three proteins, we observed a loss of snoZ30 binding to Bmc1 upon knockout of any member of the complex (Supplementary Figure S2A). ------- COMMENT: eec0954c4a56c293 18 GxfJNMbwdf7V+jy6QqjocjpSUl8 Further support- ing the idea that U6 complex formation is contingent on the presence of all three proteins, we observed a loss of snoZ30 binding to Bmc1 upon knockout of any member of the complex (Supplementary Figure S2A). ------- COMMENT: eec0954c4a56c293 19 GxfJNMbwdf7V+jy6QqjocjpSUl8 Further support- ing the idea that U6 complex formation is contingent on the presence of all three proteins, we observed a loss of snoZ30 binding to Bmc1 upon knockout of any member of the complex (Supplementary Figure S2A). ------- COMMENT: eec0954c4a56c293 21 Qr7w6KVglVvFXVeAOlCgrA/Nv44 We validated the interaction between Bmc1 and sno530 by RNP immunoprecipitation/qPCR and showed that much like snoZ30 and U6, this interaction is dependent on the presence of the assembled Bmc1-Pof8-Thc1 com- plex (Figure 1A). ------- COMMENT: eec0954c4a56c293 22 T9jflvHDiJwFFze2OnsyOOmbrko Deletion of snoZ30 and sno530 resulted in a loss of 2′-O-methylation at A41 and A64, respectively, suggesting that sno530 is indeed the A64 U6-modifying snoRNA (Figure 1C, D, Supplementary Figure S3). ------- COMMENT: eec0954c4a56c293 23 FunYKG6jbJgxjyiwqFbSeYqRCqM (comment: U6) ------- COMMENT: eec0954c4a56c293 25 FunYKG6jbJgxjyiwqFbSeYqRCqM (comment: U6) ------- COMMENT: eec0954c4a56c293 26 FunYKG6jbJgxjyiwqFbSeYqRCqM (comment: U6) ------- COMMENT: eec0954c4a56c293 27 Pq38kSYVlOOsTXfkD3eT94zsnio Still, as mean intron retention values indeed showed an in- crease upon Bmc1 deletion (Figure 5A), we chose several representative intron retention events to validate with semi- quantitative RT-PCR (one of which, intron 1 of pud1, dis- played a statistically significant increase upon Bmc1 dele- tion at 32 ̊C in our RNA Seq dataset). ------- COMMENT: eec0954c4a56c293 28 Pq38kSYVlOOsTXfkD3eT94zsnio Still, as mean intron retention values indeed showed an in- crease upon Bmc1 deletion (Figure 5A), we chose several representative intron retention events to validate with semi- quantitative RT-PCR (one of which, intron 1 of pud1, dis- played a statistically significant increase upon Bmc1 dele- tion at 32 ̊C in our RNA Seq dataset). ------- COMMENT: eec0954c4a56c293 29 6eIrURGfSYWP6JitGt2tWnqqNCs (comment: Bmc1 5 capping catalytic activity is not required for promoting 2 -O-methylation of U6) ------- COMMENT: eec560c358000716 1 PE/TLWwcBNbXDhwMSzGl0dne8EU (Fig. 1A) ------- COMMENT: eec560c358000716 2 gDQNLUUCeMoLtTtz12wPo2fecc0 (Fig. 1B) ------- COMMENT: eec560c358000716 3 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: eec560c358000716 4 p3x2dDTjQlS9jbO+IMHpHbED4nw (Fig. 1D) ------- COMMENT: eec560c358000716 5 B5JM2yBU4BK6NUhw62i3MqZFLjI (Fig. 1E) ------- COMMENT: eec560c358000716 6 SvlFFz9CaCJByQRBTLvdFnPqSYg (Fig. 1F) ------- COMMENT: eec560c358000716 7 biv5vzYpNRqMrXY+5I3wXOO1GLA (Fig. 1G) ------- COMMENT: eec560c358000716 8 xnWwtg3TqqhqbUHXva5kkxV96Hs (Fig. 1H) ------- COMMENT: eec560c358000716 9 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: eec560c358000716 10 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: eec560c358000716 11 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: eec560c358000716 12 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: eec560c358000716 13 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: eec560c358000716 14 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: eec560c358000716 15 Q59knxh8pPVXgOMltrfQPfn+cCw (Fig. 3, Fig. 6) ------- COMMENT: eec560c358000716 16 u9c4nGqbTf7MvI8SzbIpjU9a6s8 (Fig. 3B, 3C, Fig. 4, Fig. 5) ------- COMMENT: eec560c358000716 17 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: eec560c358000716 18 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: eec560c358000716 19 j6G2s1mlHnX+grtKdaKc3y1QZCw (Fig. 8B) ------- COMMENT: eec560c358000716 20 j6G2s1mlHnX+grtKdaKc3y1QZCw (Fig. 8B) ------- COMMENT: eec560c358000716 21 TQswKTQbVy4BmacYDTi4A/UGKgI (Fig. 8) ------- COMMENT: eef07d879f4207d2 3 PjiSi/0Bflr4Mo0uvf3hiAb7wwg (comment: CHECK during G2 phase of mitotic cell cycle) ------- COMMENT: eef07d879f4207d2 4 PjiSi/0Bflr4Mo0uvf3hiAb7wwg (comment: CHECK during G2 phase of mitotic cell cycle) ------- COMMENT: eef07d879f4207d2 5 PjiSi/0Bflr4Mo0uvf3hiAb7wwg (comment: CHECK during G2 phase of mitotic cell cycle) ------- COMMENT: eef07d879f4207d2 6 PjiSi/0Bflr4Mo0uvf3hiAb7wwg (comment: CHECK during G2 phase of mitotic cell cycle) ------- COMMENT: eef07d879f4207d2 7 PShmReelpXsNeaytbgztv58TNqI (comment: CHECK during cytokinesis) ------- COMMENT: ef012afa9234a01e 1 FvILjjkxji2mIPATY3dwrLiZNgs Figure 2...The uvde gene disruption made cells only mildly sensitive to UV, even after high doses. ------- COMMENT: ef012afa9234a01e 2 wukYuedkz2kkZJwZrCKBzDAxomE (comment: Cell survival assay) ------- COMMENT: ef012afa9234a01e 3 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: ef012afa9234a01e 4 wukYuedkz2kkZJwZrCKBzDAxomE (comment: Cell survival assay) ------- COMMENT: ef012afa9234a01e 5 wukYuedkz2kkZJwZrCKBzDAxomE (comment: Cell survival assay) ------- COMMENT: ef012afa9234a01e 8 VelIhjA5cHJ573UkjsVEJ+22LzU We found that the double mutant uvded rad2d was more resistant than a rad2d single mutant (Fig. 3). ------- COMMENT: ef012afa9234a01e 9 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: ef012afa9234a01e 10 8xuqiR/eRr82mfwOek6eQBtdFgw Figure 4. The rad13d uvded double mutant cells were unable to remove either type of damage, even during 3 h post-UV incubation. ------- COMMENT: ef012afa9234a01e 11 5857N8QaAgsQSEY4E3mkb7xhi4Q We found that the double mutant uvded rad2d was more resistant than a rad2d single mutant (Fig. 3). This implies that the Rad2 protein is very important for processing nicks introduced by UVDE..........These results show that the Rad2 protein is involved only in the UVDE-mediated second pathway and that there are both rad2-dependent and rad2-independent components of the UVDE-mediated repair pathway. ------- COMMENT: ef012afa9234a01e 13 i6cS1cJ6Zsqko3PDw1SMH7PWZqQ Furthermore, a rad13d uvded rad2d triple disruptant had the same UV sensitivity as the rad13d uvded double disruptant. ------- COMMENT: ef012afa9234a01e 14 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: ef4650dd0b276332 1 /TMD/VwmBPZPpiltxNWfj+RvSm4 Figure 1. ------- COMMENT: ef4650dd0b276332 2 /TMD/VwmBPZPpiltxNWfj+RvSm4 Figure 1. ------- COMMENT: ef4650dd0b276332 3 /TMD/VwmBPZPpiltxNWfj+RvSm4 Figure 1. ------- COMMENT: ef4650dd0b276332 4 /TMD/VwmBPZPpiltxNWfj+RvSm4 Figure 1. ------- COMMENT: ef4650dd0b276332 5 /TMD/VwmBPZPpiltxNWfj+RvSm4 Figure 1. ------- COMMENT: ef4650dd0b276332 6 /TMD/VwmBPZPpiltxNWfj+RvSm4 Figure 1. ------- COMMENT: ef4650dd0b276332 7 /TMD/VwmBPZPpiltxNWfj+RvSm4 Figure 1. ------- COMMENT: ef4650dd0b276332 8 /TMD/VwmBPZPpiltxNWfj+RvSm4 Figure 1. ------- COMMENT: ef4650dd0b276332 9 /TMD/VwmBPZPpiltxNWfj+RvSm4 Figure 1. ------- COMMENT: ef4650dd0b276332 10 1uEOcFf8lGNAEmweMU80cSD2nEU Figure 3. ------- COMMENT: ef4650dd0b276332 11 1uEOcFf8lGNAEmweMU80cSD2nEU Figure 3. ------- COMMENT: ef4650dd0b276332 12 1uEOcFf8lGNAEmweMU80cSD2nEU Figure 3. ------- COMMENT: ef4650dd0b276332 13 1uEOcFf8lGNAEmweMU80cSD2nEU Figure 3. ------- COMMENT: ef4650dd0b276332 14 1uEOcFf8lGNAEmweMU80cSD2nEU Figure 3. ------- COMMENT: ef4650dd0b276332 15 1uEOcFf8lGNAEmweMU80cSD2nEU Figure 3. ------- COMMENT: ef57da759b1d97c2 39 N1YVPaJw1+/fOXVvICpOsuX1DGs (comment: no hydroxyurea) ------- COMMENT: ef61ac1ac91c951e 2 N+Hgpy3jC9FMJKdAQqyW/isMp5Y cell size at separation is 16.7µm compared to 12.8µm for wild type ------- COMMENT: ef61ac1ac91c951e 3 qz5ocOavY6JV2AtWlCa3H4skFrc cell size at separation is 22.4µm compared to 12.8µm for wild type ------- COMMENT: ef61ac1ac91c951e 4 /F+BOjXnrZDEobJKr6NpCr8kjwI cell size at septation is 9.6µm ------- COMMENT: ef61ac1ac91c951e 5 bK5axyE9D8BRaJ8dnpye2X649nc cell size at septation is 10.3µm ------- COMMENT: ef61ac1ac91c951e 6 e2GwY/DIrWHhXQ71W29H9mIZ1B0 cell size at septation is 8.4µm ------- COMMENT: ef61ac1ac91c951e 7 FC1Vj8L8QZni2D/eo8+NvxHDu2g cell size at septation is 8.9µm ------- COMMENT: ef61ac1ac91c951e 8 yPMnSqBjTQmkCzrYuZWVZS2tlB4 cell size at septation is 8.5µm ------- COMMENT: ef61ac1ac91c951e 9 FC1Vj8L8QZni2D/eo8+NvxHDu2g cell size at septation is 8.9µm ------- COMMENT: ef61ac1ac91c951e 10 iDi93kceHscFx6uwGmtxjT209Pk cell size at septation is 8.7µm ------- COMMENT: ef61ac1ac91c951e 11 MW89V6RoTgpsrV6Pz+QIutXxHIg cell size at septation is 9.5µm in cdc2-1w (9.7µm in cdc2-2w) ------- COMMENT: ef61ac1ac91c951e 35 M9iK7x+RtybHYAKQkpOrYkynKlg fig1A The cdc1 gene has a execution point of ~0.65 which means its function is completed just before entry into mitosis. An asynchronous population of the cdc1-7 mutant was shifted from 25°C to the restrictive temperature of 36°C to inactivate the gene and the number of cells that went on to divide was measured ------- COMMENT: ef61ac1ac91c951e 36 LYbCplnjZOmruknqb/zVPdVVnwU (comment: ???) ------- COMMENT: ef61ac1ac91c951e 37 lonJPpWk0L0iSsiRDx88BwWulCQ fig1A The cdc1 gene has a execution point of ~0.62 which means its function is completed just before entry into mitosis. An asynchronous population of the cdc1-7 mutant was shifted from 25°C to the restrictive temperature of 36°C to inactivate the gene and the number of cells that went on to divide was measured ------- COMMENT: ef61ac1ac91c951e 38 r9PKLDEKCrHNmS1mOjT5KesSA6o (comment: TP. 0.33) ------- COMMENT: ef61ac1ac91c951e 39 gpQLb+IXHQ/A1Z1JXiCyl4X2k2s fig1A The transition point s advanced from 0.68 to 0.29 in a cdc1.7 wee1.6 mutant. An asynchronous population of a cdc1-7 wee1.6 mutant was shifted from 25°C to the restrictive temperature of 36°C to inactivate the cdc1 gene and the number of cells that went on to divide was increased compared to a cdc1-7 mutant ------- COMMENT: ef61ac1ac91c951e 40 C1pKkWmNqHn+jtPC2KYFNGvujlI fig1A (comment: EXP) The transition point for cdc1 is not advanced in a cdc1.7 cdc2-1w mutant (0.64). An asynchronous population of a cdc1-7cdc2-1w mutant was shifted from 25°C to the restrictive temperature of 36°C to inactivate the cdc1 gene and the number of cells that went on to divide was similar to a cdc1-7 mutant. The transition point for cdc1 (0.58) is not advanced in a cdc1.7 cdc2-2w mutant (comment: cdc2-2w is the same change as cdc2-1w, but also consider comment transferred from duplicate annotation: "I think this allele cdc2-2w maybe a typo or a changed annotation since this paper was published and is actually cdc2-3w"). ------- COMMENT: ef61ac1ac91c951e 42 TXnMEhNm5QVsMHqtlin3XvgJfZQ fig1A, Table 1 cdc27 transition point is 0.62 using a cdc27.K3 mutant ------- COMMENT: ef61ac1ac91c951e 43 BJ9agz6y6b/NgpAgJ67McOvQ98c fig1A The transition point for cdc27 is advanced from 0.63 to 0.22 in a cdc27-K3 wee1.6 mutant. An asynchronous population of a cdc1-7 wee1.6 mutant was shifted from 25°C to the restrictive temperature of 36°C to inactivate the cdc27 gene and the number of cells that went on to divide was increased compared to a cdc27-K3 mutant ------- COMMENT: ef61ac1ac91c951e 44 mTXP54VGk0B7EW9Fm68zFko4bHU fig1A The transition point for cdc2 is 0.65 using cdc2.33 ------- COMMENT: ef61ac1ac91c951e 45 C4/pM5oIpuMUsBzBVreemOhdtMg fig1A The transition point for cdc2 is 0.68 using cdc2.L7 ------- COMMENT: ef61ac1ac91c951e 46 5QXlDZS/4V9IuB3CtWq1Hpo/I+Y fig1A The transition point for cdc2 is 0.70 using cdc2.M63 ------- COMMENT: ef61ac1ac91c951e 47 7TAGBGw9Yo/bkjOHSQVe08uM8uw fig1A The transition point for cdc2 is 0.65 using cdc2.M26 ------- COMMENT: ef61ac1ac91c951e 48 YVX9mCgy3u7g85IK2xH68f941Gg fig1A The transition point for cdc2 is 0.66 using cdc2.M35 ------- COMMENT: ef61ac1ac91c951e 49 9704NBfy+b3HJub4+bbba4AvUzs fig1A The transition point for cdc2 is 0.65 using cdc2.M55 ------- COMMENT: ef61ac1ac91c951e 50 dlVFpTeO4jXUVYPwZOnggl3UKuA fig1A The transition point for cdc2 is advanced from 0.69 to 0.48 using a cdc2.33 wee1 [more...] ------- COMMENT: ef61ac1ac91c951e 52 C2w/r+3/aNgvG29Vb61PF6Wi4Go The transition point for cdc2 is advanced from 0.68 to 0.47 using a cdc2.L7 wee1.6 mutant ------- COMMENT: ef61ac1ac91c951e 53 YrFzNnPMC7SMfHVYja+zF77fPuw The transition point for cdc2 is advanced from 0.65 to 0.53 using a cdc2.M26 wee1.6 mutant ------- COMMENT: ef61ac1ac91c951e 54 kFxTKIIEF3aP3Rk7ShFaLMHeEWA fig1A ------- COMMENT: ef61ac1ac91c951e 55 xmSoKbQGXK/+Nbua7hqA2TWEom8 fig1A The transition point for cdc2 (0.68) is not advanced using a cdc2.M63 wee1.6 mutant ------- COMMENT: ef61ac1ac91c951e 56 W6koxfA8IuVkPoDlbk9dhG/zVvo The transition point for cdc1 is advanced in a cdc1.P13 wee1.6 mutant from 0.62 to 0.33. An asynchronous population of a cdc1-P13 wee1.6 mutant was shifted from 25°C to the restrictive temperature of 36°C to inactivate the cdc1 gene and the number of cells that went on to divide was increased compared to a cdc1-7 mutant ------- COMMENT: ef61ac1ac91c951e 57 nyJmURyrZa/UdSFebGCF+/qFNFs fig1A, Table 1 cdc13 transition point is 0.69 using a cdc13-117 mutant ------- COMMENT: ef61ac1ac91c951e 58 524YEz6tQM3MmbPMMyiT+Lnk4QU fig1A, Table 1 cdc13 transition point (0.78) is not advanced in a cdc13-117 wee1.6 mutant ------- COMMENT: ef61ac1ac91c951e 59 HcaEqgks8Q/4qWFM62YtyYfXBak fig1A The transition point for cdc2 (0.74) is not advanced using a cdc2.M35 wee1.6 mutant ------- COMMENT: ef8c7a4f76471904 1 8WhfSyeP9AEquIQKIfeoXkhITxc (comment: mat3M::ura4+ reporter silencing) ------- COMMENT: ef8c7a4f76471904 2 8WhfSyeP9AEquIQKIfeoXkhITxc (comment: mat3M::ura4+ reporter silencing) ------- COMMENT: ef8c7a4f76471904 3 X/leYqWi2rmLpfNr+NnymtL3Nj4 (comment: mat3M::ura4+ reporter silencing) |combined mutations of the hinge and one of the mutations in the N-terminus of CSD (mut3 and mut5) showed a silencing defect (Fig. 4C) ------- COMMENT: ef8c7a4f76471904 4 yyLd+C8ZuWE6GS+4rhjR5WXKwBs Chp2 mutants lacking DNA-binding activities associated with both the hinge and the N-terminus of CSD (mut 3 and mut 5) exhibited reduced heterochromatin association compared to wild-type Chp2 at representative heterochromatic regions (centromeric dg, the mating-type cenH, telomere and mat3M::ura4+), and the reduction was more severe for Chp2-mut3 compared to Chp2-mut5 (Fig. 6A). ------- COMMENT: ef8c7a4f76471904 5 8WhfSyeP9AEquIQKIfeoXkhITxc (comment: mat3M::ura4+ reporter silencing) ------- COMMENT: ef8c7a4f76471904 6 f51PanQrvcnyr1ST1yB34BcfP6o (comment: mat3M::ura4+ reporter silencing )| combined mutations of the hinge and one of the mutations in the N-terminus of CSD (mut3 and mut5) showed a silencing defect (Fig. 4C) ------- COMMENT: ef8c7a4f76471904 7 yyLd+C8ZuWE6GS+4rhjR5WXKwBs Chp2 mutants lacking DNA-binding activities associated with both the hinge and the N-terminus of CSD (mut 3 and mut 5) exhibited reduced heterochromatin association compared to wild-type Chp2 at representative heterochromatic regions (centromeric dg, the mating-type cenH, telomere and mat3M::ura4+), and the reduction was more severe for Chp2-mut3 compared to Chp2-mut5 (Fig. 6A). ------- COMMENT: ef8c7a4f76471904 8 f51PanQrvcnyr1ST1yB34BcfP6o (comment: mat3M::ura4+ reporter silencing )| combined mutations of the hinge and one of the mutations in the N-terminus of CSD (mut3 and mut5) showed a silencing defect (Fig. 4C) ------- COMMENT: ef8c7a4f76471904 9 yyLd+C8ZuWE6GS+4rhjR5WXKwBs Chp2 mutants lacking DNA-binding activities associated with both the hinge and the N-terminus of CSD (mut 3 and mut 5) exhibited reduced heterochromatin association compared to wild-type Chp2 at representative heterochromatic regions (centromeric dg, the mating-type cenH, telomere and mat3M::ura4+), and the reduction was more severe for Chp2-mut3 compared to Chp2-mut5 (Fig. 6A). ------- COMMENT: ef8c7a4f76471904 10 EW4UV5QbLs4o3HAYB9mRD4c9xls As previously reported, in wild-type cells, Swi6 was present in both the soluble (S) and chromatin-enriched pellet (P) fractions, with approximately 40% of the total Swi6 protein detected in the pellet fraction, and most of the Swi6 in the pellet fraction was redistributed to the soluble fraction in clr4 cells (Fig. 1A). ------- COMMENT: ef8c7a4f76471904 11 Bm7bOBubsd44nBJdu8vWGWtIN1Y In contrast, Chp2 was preferentially present in the chromatin-enriched pellet fraction in wild-type cells, and the Chp2 in this fraction was not altered by the clr4+ depletion (Fig. 1B). ------- COMMENT: ef8c7a4f76471904 12 Wd/druV5X1eG+gxFgUCsBTT42/o Interest- ingly, we found that Chp2 in the chromatin-enriched pellet fraction was not affected by the Mit1I11R mutation (Fig. 1B). ------- COMMENT: ef8c7a4f76471904 13 pvAO8q1myvBdedUkHGD/Ml59IFg Using purified full-length, dimerized Chp2 and Swi6, we performed EMSAs using pericentromeric DNA as a probe. Consistent with previous results, Swi6 bound DNA efficiently, and Chp2 also showed similar DNA binding activity (Fig. 2B and C). ------- COMMENT: ef8c7a4f76471904 14 pvAO8q1myvBdedUkHGD/Ml59IFg Using purified full-length, dimerized Chp2 and Swi6, we performed EMSAs using pericentromeric DNA as a probe. Consistent with previous results, Swi6 bound DNA efficiently, and Chp2 also showed similar DNA binding activity (Fig. 2B and C). ------- COMMENT: ef8c7a4f76471904 16 Pga2uAvrAHqMRTjUtWjYjUquh2s (comment: CHECK ****NEED TO FIX allele description*****.) The N-terminal disordered region of Swi6 (Swi6-N) bound weakly to DNA (Fig. 2D and L), ------- COMMENT: ef8c7a4f76471904 17 u/9qxKieZM+KO9txMJKf4UfRCxc whereas no DNA binding activity was detected for Swi6-CD or Swi6-CSD (Fig. 2E, G and L). ------- COMMENT: ef8c7a4f76471904 18 u/9qxKieZM+KO9txMJKf4UfRCxc whereas no DNA binding activity was detected for Swi6-CD or Swi6-CSD (Fig. 2E, G and L). ------- COMMENT: ef8c7a4f76471904 19 K5sLJcpxF5tb7mthoQrrlX4I15s The hinge and the N-terminal disor- dered regions of Chp2 (Chp2-H and Chp2-N) also bound DNA (Fig. 2H, J and M) ------- COMMENT: ef8c7a4f76471904 20 K5sLJcpxF5tb7mthoQrrlX4I15s The hinge and the N-terminal disor- dered regions of Chp2 (Chp2-H and Chp2-N) also bound DNA (Fig. 2H, J and M) ------- COMMENT: ef8c7a4f76471904 21 7qsmTlQ1quyB0qd3fuDmwhQpgbk no detectable DNA-binding activity was observed for Chp2-CD (Fig. 2I and M). ------- COMMENT: ef8c7a4f76471904 22 g63KmZmBiFOQmbNz/+cY//Sg5lI Inter- estingly, we found that Chp2-CSD exhibited a robust DNA binding activity (Fig. 2K and M), ------- COMMENT: ef8c7a4f76471904 23 l49xHu/HEaltskF7IEEZr1TeF4s when these were replaced by alanine (Fig. 3A), the resulting Chp2-H mutant (Chp2-H5A) no longer bound to DNA (Fig. 3B and C, and Supplementary Fig. S2A and S2D), ------- COMMENT: ef8c7a4f76471904 24 kX3QFmi6YWGZqiBMnH/2XcMUGwE exhibited only a very weak DNA binding activity compared to wild- type Chp2-CD (Chp2-CSDWT ) (Fig. 3D and E, and Supple- mentary Fig. S2B and S2E) ------- COMMENT: ef8c7a4f76471904 25 29n38IRU3PSHsUQpUXUdR5446RM Chp2-CSD (Chp2-CSD3A) no longer bound DNA (Fig. 3F and Supplementary Fig. S2B), Chp2 with H5A mutation in the hinge (Chp2-mut1) or with either CSD2A or CSD3A mutation in the CSD (Chp2-mut2 and Chp2- mut4) exhibited weaker DNA-binding activity compared to wild-type Chp2 (Chp2-WT) (Fig. 3H, I, J and L and Sup- plementary Fig. S2C and S2F) ------- COMMENT: ef8c7a4f76471904 26 q7NRLuTxz/BzunMkOsP3wK7iFOY Interestingly, when amino acid substitutions in the hinge and CSD were combined, the resulting Chp2 mutants (Chp2-mut3 and Chp3-mut5) no longer bound DNA (Fig. 3K and M, and Supplementary Fig. S2F) ------- COMMENT: ef8c7a4f76471904 27 q7NRLuTxz/BzunMkOsP3wK7iFOY Interestingly, when amino acid substitutions in the hinge and CSD were combined, the resulting Chp2 mutants (Chp2-mut3 and Chp3-mut5) no longer bound DNA (Fig. 3K and M, and Supplementary Fig. S2F) ------- COMMENT: efb40309f7d22a73 1 BH/7D5dtcLFZp6BsX5LfFjfjGtk (comment: also inferred from chromatin localization and reporter gene expression) ------- COMMENT: efb40309f7d22a73 8 EBDv7VmUtIZgPR1jFPxzdHMHiUc represses Pho7-mediated transcription activationin phosphate-replete conditions; does not regulate Pho7 DNA binding ------- COMMENT: efb40309f7d22a73 19 n6iE98an4Uy8ZjU49FtD+asfdyI (comment: CHECK at pho1+ and SPBC1271.09) ------- COMMENT: efc5a5e85ec7f642 1 ssIzB0yxzE8PTotYAmwzz9n/wpc Figure 1A, Sup Fig1 ------- COMMENT: efc5a5e85ec7f642 2 8os03f7UBAnOs6tRq2AtWrLkM7E (comment: jack suggested "up regulation of protein binding RNAs because normally bound by zfs1" I'm using this to make the sequestering GO annotation.) ------- COMMENT: efc5a5e85ec7f642 3 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: efc5a5e85ec7f642 4 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: efc5a5e85ec7f642 5 p4n0y2pMxpInJZvoqbEAhlhzApM Fig 2B, 2C, fig6a ------- COMMENT: efc5a5e85ec7f642 6 W+YSRV/nqaaGqtvxitToWAjlPhs Fig 2D, 2E, Sup Fig 2 ------- COMMENT: efc5a5e85ec7f642 7 oT/OcXiSsH6RWhVLJJEUtUifcUE Fig3A ------- COMMENT: efc5a5e85ec7f642 8 8F9feOThcAguyM35YM4T0ey4XHI Fig3A deletion of puc1 increases mating efficiency of zfs1 delta to WT ------- COMMENT: efc5a5e85ec7f642 9 8sqZabRhFIKVW/d4FFueg9EOw2k Fig3B varying the copy number of pJKpuc1+ leads to varying levels of sporulation efficiency in wild type cells increased puc1 mRNA causes reduced mating efficiency ------- COMMENT: efc5a5e85ec7f642 10 DilKHrsqJvkM9GHi5AVQ+kPdqbU (comment: greater than 4 fold enrichment IP/input) ------- COMMENT: efc5a5e85ec7f642 11 DilKHrsqJvkM9GHi5AVQ+kPdqbU (comment: greater than 4 fold enrichment IP/input) ------- COMMENT: efc5a5e85ec7f642 12 DilKHrsqJvkM9GHi5AVQ+kPdqbU (comment: greater than 4 fold enrichment IP/input) ------- COMMENT: efc5a5e85ec7f642 13 EAJZ4JkjhhnnzDDDdPQ9joFPZhI (comment: about 2 fold enrichment) ------- COMMENT: efc5a5e85ec7f642 14 hKWCsgQ9BP4CeUjS4JB+UtD3J0c Fig3C ------- COMMENT: efc5a5e85ec7f642 15 hKWCsgQ9BP4CeUjS4JB+UtD3J0c Fig3C ------- COMMENT: efc5a5e85ec7f642 16 bWAlNCfwL+wEjS/JpYynzeKo/r4 Fig3C, Fig7A shows that zfs1delta shows high mating efficiency at nitrogen levels which suppress mating in wild type cells wild type cells ------- COMMENT: efc5a5e85ec7f642 17 IfuxNtX+Bcoqc9M4a6PFyL/d5dM Fig4A, 4B Zfs is hyperphosphorylated in response to nitrogen depletion ------- COMMENT: efc5a5e85ec7f642 18 Dl5EGy4vHxlZ22hj1V6u+pz6lko Fig4D, Sup Fig3 the hyperphosphoryated zfs1 (3rd band higest). Gad8 is required to hyperphosphorylate zfs1. In Fig4C they also show that TOR inhibition by Torin stimulates hyerphosphorylation of Zfs1 ------- COMMENT: efc5a5e85ec7f642 19 fVvpes0LTGPBvf8qU3ro3uBcR3k Fig4A, 4B (comment: about 10%? of Zfs1 is phosphorylated during vegetative growth) ------- COMMENT: efc5a5e85ec7f642 20 vwK2CkRZLA/QlLo/ipNO7ecHP98 Fig 5B, construct 3 ------- COMMENT: efc5a5e85ec7f642 21 /gMLWwuOKWFJUE5KuvqhCJ+XZFs Fig5C, 5D ------- COMMENT: efc5a5e85ec7f642 22 /gMLWwuOKWFJUE5KuvqhCJ+XZFs Fig5C, 5D ------- COMMENT: efc5a5e85ec7f642 23 uvbBK+s2qhPo7lggB0RT0vX79gY Fig5E ------- COMMENT: efc5a5e85ec7f642 24 uvbBK+s2qhPo7lggB0RT0vX79gY Fig5E ------- COMMENT: efc5a5e85ec7f642 26 VN4N+vW75GMJ++O3CfyecQbICco Fig6A ------- COMMENT: efc5a5e85ec7f642 27 VN4N+vW75GMJ++O3CfyecQbICco Fig6A ------- COMMENT: efc5a5e85ec7f642 28 VN4N+vW75GMJ++O3CfyecQbICco Fig6A ------- COMMENT: efc5a5e85ec7f642 29 ROtrA44QfG8vGVjFfBul/AgxTYg Fig6A, 6B ------- COMMENT: efc5a5e85ec7f642 30 h9nnIMbNhlreJdG+kFfinwZgCWM reduced binding compared to control ------- COMMENT: efc5a5e85ec7f642 31 h9nnIMbNhlreJdG+kFfinwZgCWM reduced binding compared to control ------- COMMENT: efc5a5e85ec7f642 32 OaQhhp0NxGcxrkJYqoaN79CaVBs Fig3A deletion of cig1 does not rescue mating efficiency of zfs1 delta ------- COMMENT: efc5a5e85ec7f642 33 yhy6twDjwjGgl7y+skl3tSvl8Qs Fig3A deletion of cig2 does not rescue mating efficiency zfs1delta ------- COMMENT: efc5a5e85ec7f642 34 uvbBK+s2qhPo7lggB0RT0vX79gY Fig5E ------- COMMENT: efc5a5e85ec7f642 35 uvbBK+s2qhPo7lggB0RT0vX79gY Fig5E ------- COMMENT: efc5a5e85ec7f642 36 VN4N+vW75GMJ++O3CfyecQbICco Fig6A ------- COMMENT: efc5a5e85ec7f642 37 VN4N+vW75GMJ++O3CfyecQbICco Fig6A ------- COMMENT: efc5a5e85ec7f642 38 ROtrA44QfG8vGVjFfBul/AgxTYg Fig6A, 6B ------- COMMENT: efc5a5e85ec7f642 39 EV2xy/148dj1cXvZ2oYTohQ44QQ Fig6A, 6B ------- COMMENT: efc5a5e85ec7f642 40 ROtrA44QfG8vGVjFfBul/AgxTYg Fig6A, 6B ------- COMMENT: efc5a5e85ec7f642 41 ROtrA44QfG8vGVjFfBul/AgxTYg Fig6A, 6B ------- COMMENT: efc5a5e85ec7f642 42 ROtrA44QfG8vGVjFfBul/AgxTYg Fig6A, 6B ------- COMMENT: efc5a5e85ec7f642 43 13Y3V1zuxopLtH7FEh0/VlppAXQ Fig7A shows that puc1delta shows increased mating efficiency at nitrogen levels which suppress mating in wild type cells ------- COMMENT: efc5a5e85ec7f642 47 2ZoX6+HdbbbyPVZ7PorYIrf5RnY (comment: RNA) ------- COMMENT: eff3b387846d7c66 1 /ltlKPX9e6vWeKs5TRaUS2MhikY figure 2 ------- COMMENT: eff3b387846d7c66 2 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: eff3b387846d7c66 3 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: eff3b387846d7c66 4 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: eff3b387846d7c66 5 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: eff3b387846d7c66 6 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: eff3b387846d7c66 7 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: eff3b387846d7c66 8 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: eff3b387846d7c66 9 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: eff3b387846d7c66 10 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: eff3b387846d7c66 11 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: eff3b387846d7c66 12 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: eff3b387846d7c66 13 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: eff3b387846d7c66 14 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: eff3b387846d7c66 15 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: eff3b387846d7c66 16 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: eff3b387846d7c66 17 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: eff3b387846d7c66 18 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: eff3b387846d7c66 19 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: eff3b387846d7c66 20 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: eff3b387846d7c66 21 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: eff3b387846d7c66 22 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: eff3b387846d7c66 23 MnMN85C8dU0+03GXPaZOu85OHwA Figure 3 (comment: CHECK E3) ------- COMMENT: eff3b387846d7c66 24 2RIXP2WsyBz3/AL9pTomyXRq0mk Figure 3 (comment: CHECK E2) ------- COMMENT: eff3b387846d7c66 25 IE2xtB5sE2dS1DdUzJ73G07eSeI Figure 3 (comment: CHECK E1, activating) ------- COMMENT: f02a15cea2c40c03 16 klHyiheef2Flb6XsXRwAkbKqqM8 (comment: also uses Pol ii-RNA immunoprecipitation) ------- COMMENT: f02a15cea2c40c03 17 zWPYOtbaEgSqbWFx1vClMi6ghEY (comment: uses Pol ii-RNA immunoprecipitation) ------- COMMENT: f02a15cea2c40c03 23 klHyiheef2Flb6XsXRwAkbKqqM8 (comment: also uses Pol ii-RNA immunoprecipitation) ------- COMMENT: f02a15cea2c40c03 24 zWPYOtbaEgSqbWFx1vClMi6ghEY (comment: uses Pol ii-RNA immunoprecipitation) ------- COMMENT: f02a15cea2c40c03 25 qTYGfvw7mmvCWRd0ng2s55AqKio (comment: sequencing of Ago1-bound siRNA) ------- COMMENT: f02a15cea2c40c03 26 qTYGfvw7mmvCWRd0ng2s55AqKio (comment: sequencing of Ago1-bound siRNA) ------- COMMENT: f02a15cea2c40c03 36 qTYGfvw7mmvCWRd0ng2s55AqKio (comment: sequencing of Ago1-bound siRNA) ------- COMMENT: f02a15cea2c40c03 37 qTYGfvw7mmvCWRd0ng2s55AqKio (comment: sequencing of Ago1-bound siRNA) ------- COMMENT: f02a15cea2c40c03 38 qTYGfvw7mmvCWRd0ng2s55AqKio (comment: sequencing of Ago1-bound siRNA) ------- COMMENT: f02a15cea2c40c03 39 qTYGfvw7mmvCWRd0ng2s55AqKio (comment: sequencing of Ago1-bound siRNA) ------- COMMENT: f02a15cea2c40c03 45 oFsG6Ny4cLu8m+LWVv1V4ZSShZI (comment: uses histone H3 RNA immunoprecipitation) ------- COMMENT: f02a15cea2c40c03 49 qTYGfvw7mmvCWRd0ng2s55AqKio (comment: sequencing of Ago1-bound siRNA) ------- COMMENT: f0575bfe108d54e1 1 Mc1Dl/8ePjG+W3BQIevC1vaEvqw Figure 2D ------- COMMENT: f0575bfe108d54e1 2 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: f0575bfe108d54e1 3 8zdno63XFs3BFsVP161LRtfCMNM Figure 3D ------- COMMENT: f0575bfe108d54e1 4 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: f0575bfe108d54e1 5 HQabJHserfLRswU1bIqlCoxKeyE Figure 4B ------- COMMENT: f0575bfe108d54e1 6 wf185u4DRDkg7Sbpsn3gLe6+2NY Figure 4E ------- COMMENT: f0575bfe108d54e1 15 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: f0575bfe108d54e1 16 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: f0575bfe108d54e1 17 rq1ZnTXxSj5ScGqdjunaigzasY0 Fig. 2B In moa1Δ cells, a mi- nority population (11%) of cells underwent equational segregation at meiosis I (because of defects in mono-orientation), whereas the majority underwent reductional segregation due to the presence of chiasmata and tension exerted across homologs, as reported previously (Miyazaki et al, 2017) (Fig 2B). ------- COMMENT: f0575bfe108d54e1 18 wD4gieg3zo/7veye9eR20ZeRbNw (comment: CHECK Unequal sister chromatid segregation in meiosis II after reductional segregation in meiosis I.) Fig. 2B ------- COMMENT: f0575bfe108d54e1 19 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f0575bfe108d54e1 20 Mc1Dl/8ePjG+W3BQIevC1vaEvqw Figure 2D ------- COMMENT: f0575bfe108d54e1 21 Mc1Dl/8ePjG+W3BQIevC1vaEvqw Figure 2D ------- COMMENT: f0575bfe108d54e1 22 gGO8PhPdglxrWk/zV9TfIovMH8M Figure 2D emarkably, introducing the rec8-15A mutation into rec12Δ rec8-2A cells increased equational segrega- tion to 36% (Fig 2D), suggesting that phosphorylation at some or all 15S/T sites in Rec8 is contributing at least partly to establishing mono-orientation, most likely by promoting cohesion at the core centromeres. ------- COMMENT: f0575bfe108d54e1 23 qaR7w+504riSNowBJJS0NJe0XiM We identified 11 polo-kinase consensus N/Q/E/D-X-S/T sites and four non-consensus S/T in this domain, which were phosphorylated by Plo1 in vitro and some of them were detected also in vivo (Fig S1A–C). ------- COMMENT: f0575bfe108d54e1 24 SFckLJW3x7fQnQdREcyvezuxZjI Furthermore, we examined the in vitro phosphorylation by Plo1 using recombinant Mis4 protein and found that the N terminus of Mis4 is preferentially phosphorylated by Plo1 (Fig S2A–C). ------- COMMENT: f0575bfe108d54e1 25 A2zRcAAP3BMpPAD7yXlgRFwprjg (comment: N-terminal 245aa part of Rec8 is sufficient for this interaction) (Fig. 2F) ------- COMMENT: f0575bfe108d54e1 27 ZoC6SHZyvAcnULruGq0+yJ6gAoQ (comment: Measured in two-hybrid assay using only the N-terminal 245aa part of Rec8) (Fig. 2F) ------- COMMENT: f0575bfe108d54e1 28 5Lf82Ue1kp90xxOmhbHLTZcTHdw We examined if Psm3 is phosphorylated by Plo1 in vitro and found phosphorylation of T182 in the N terminus and S1001 in the C terminus (Fig. S3) ------- COMMENT: f0575bfe108d54e1 29 cLap5aYasXwCkz7rGPgxkWxI5KE (comment: Unequal sister chromatid segregation in meiosis II after reductional segregation in meiosis I.) Fig. 3D ------- COMMENT: f0575bfe108d54e1 30 8zdno63XFs3BFsVP161LRtfCMNM Figure 3D ------- COMMENT: f0575bfe108d54e1 31 cLap5aYasXwCkz7rGPgxkWxI5KE (comment: Unequal sister chromatid segregation in meiosis II after reductional segregation in meiosis I.) Fig. 3D ------- COMMENT: f0575bfe108d54e1 32 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: f0575bfe108d54e1 33 Q+q8eGr0OxY5nyThGr/pk9fy2LY Fig. 3E ------- COMMENT: f0575bfe108d54e1 34 tOmOPs9JendY5r39VoSXnsV78ik Fig. 3F ------- COMMENT: f0575bfe108d54e1 35 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: f0575bfe108d54e1 36 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: f0575bfe108d54e1 37 tYQpfuT6Jf2Q0i+6gfohYgCKKdk Fig. 4D ------- COMMENT: f0575bfe108d54e1 38 wf185u4DRDkg7Sbpsn3gLe6+2NY Figure 4E ------- COMMENT: f0575bfe108d54e1 39 wf185u4DRDkg7Sbpsn3gLe6+2NY Figure 4E ------- COMMENT: f0575bfe108d54e1 40 wf185u4DRDkg7Sbpsn3gLe6+2NY Figure 4E ------- COMMENT: f0575bfe108d54e1 41 wf185u4DRDkg7Sbpsn3gLe6+2NY Figure 4E ------- COMMENT: f0575bfe108d54e1 42 hBvR2p78vSNVO+O1Sy182u3naQ4 segregation defects in psm3-3A in achiasmatic meiosis I suppressed by wpl1 deletion. Figure 4E ------- COMMENT: f0575bfe108d54e1 43 DfIETTB8Q01O7/5nG1VIrV+OWdU Fig. 1B In moa1Δ cells, Rec8 cohesin localization increases at the core centromere although sister chromatid cohesion is abol- ished at this sit ------- COMMENT: f0575bfe108d54e1 44 qaR7w+504riSNowBJJS0NJe0XiM We identified 11 polo-kinase consensus N/Q/E/D-X-S/T sites and four non-consensus S/T in this domain, which were phosphorylated by Plo1 in vitro and some of them were detected also in vivo (Fig S1A–C). ------- COMMENT: f0575bfe108d54e1 45 qaR7w+504riSNowBJJS0NJe0XiM We identified 11 polo-kinase consensus N/Q/E/D-X-S/T sites and four non-consensus S/T in this domain, which were phosphorylated by Plo1 in vitro and some of them were detected also in vivo (Fig S1A–C). ------- COMMENT: f0575bfe108d54e1 46 qaR7w+504riSNowBJJS0NJe0XiM We identified 11 polo-kinase consensus N/Q/E/D-X-S/T sites and four non-consensus S/T in this domain, which were phosphorylated by Plo1 in vitro and some of them were detected also in vivo (Fig S1A–C). ------- COMMENT: f0575bfe108d54e1 47 qaR7w+504riSNowBJJS0NJe0XiM We identified 11 polo-kinase consensus N/Q/E/D-X-S/T sites and four non-consensus S/T in this domain, which were phosphorylated by Plo1 in vitro and some of them were detected also in vivo (Fig S1A–C). ------- COMMENT: f0575bfe108d54e1 48 qaR7w+504riSNowBJJS0NJe0XiM We identified 11 polo-kinase consensus N/Q/E/D-X-S/T sites and four non-consensus S/T in this domain, which were phosphorylated by Plo1 in vitro and some of them were detected also in vivo (Fig S1A–C). ------- COMMENT: f0575bfe108d54e1 49 qaR7w+504riSNowBJJS0NJe0XiM We identified 11 polo-kinase consensus N/Q/E/D-X-S/T sites and four non-consensus S/T in this domain, which were phosphorylated by Plo1 in vitro and some of them were detected also in vivo (Fig S1A–C). ------- COMMENT: f0575bfe108d54e1 50 qaR7w+504riSNowBJJS0NJe0XiM We identified 11 polo-kinase consensus N/Q/E/D-X-S/T sites and four non-consensus S/T in this domain, which were phosphorylated by Plo1 in vitro and some of them were detected also in vivo (Fig S1A–C). ------- COMMENT: f0575bfe108d54e1 51 qaR7w+504riSNowBJJS0NJe0XiM We identified 11 polo-kinase consensus N/Q/E/D-X-S/T sites and four non-consensus S/T in this domain, which were phosphorylated by Plo1 in vitro and some of them were detected also in vivo (Fig S1A–C). ------- COMMENT: f0575bfe108d54e1 52 qaR7w+504riSNowBJJS0NJe0XiM We identified 11 polo-kinase consensus N/Q/E/D-X-S/T sites and four non-consensus S/T in this domain, which were phosphorylated by Plo1 in vitro and some of them were detected also in vivo (Fig S1A–C). ------- COMMENT: f0575bfe108d54e1 53 qaR7w+504riSNowBJJS0NJe0XiM We identified 11 polo-kinase consensus N/Q/E/D-X-S/T sites and four non-consensus S/T in this domain, which were phosphorylated by Plo1 in vitro and some of them were detected also in vivo (Fig S1A–C). ------- COMMENT: f0575bfe108d54e1 54 qaR7w+504riSNowBJJS0NJe0XiM We identified 11 polo-kinase consensus N/Q/E/D-X-S/T sites and four non-consensus S/T in this domain, which were phosphorylated by Plo1 in vitro and some of them were detected also in vivo (Fig S1A–C). ------- COMMENT: f0575bfe108d54e1 55 qaR7w+504riSNowBJJS0NJe0XiM We identified 11 polo-kinase consensus N/Q/E/D-X-S/T sites and four non-consensus S/T in this domain, which were phosphorylated by Plo1 in vitro and some of them were detected also in vivo (Fig S1A–C). ------- COMMENT: f0575bfe108d54e1 56 qaR7w+504riSNowBJJS0NJe0XiM We identified 11 polo-kinase consensus N/Q/E/D-X-S/T sites and four non-consensus S/T in this domain, which were phosphorylated by Plo1 in vitro and some of them were detected also in vivo (Fig S1A–C). ------- COMMENT: f0575bfe108d54e1 57 qaR7w+504riSNowBJJS0NJe0XiM We identified 11 polo-kinase consensus N/Q/E/D-X-S/T sites and four non-consensus S/T in this domain, which were phosphorylated by Plo1 in vitro and some of them were detected also in vivo (Fig S1A–C). ------- COMMENT: f0575bfe108d54e1 58 5Lf82Ue1kp90xxOmhbHLTZcTHdw We examined if Psm3 is phosphorylated by Plo1 in vitro and found phosphorylation of T182 in the N terminus and S1001 in the C terminus (Fig. S3) ------- COMMENT: f0575bfe108d54e1 59 6baW71hArnlvN1wRy7QGVID7YC0 In moa1Δ cells, a mi- nority population (11%) of cells underwent equational segregation at meiosis I (because of defects in mono-orientation), whereas the majority underwent reductional segregation due to the presence of chiasmata and tension exerted across homologs, as reported previously (Miyazaki et al, 2017) (Fig 2B) ------- COMMENT: f0575bfe108d54e1 60 S9JTGAE+83Bo01k4jesDI81NU3Q suggesting that 40% of the reductional population underwent random segregation at meiosis II which is originated from loss of cohesion (a defect in cohesion protection) in anaphase I. ------- COMMENT: f0575bfe108d54e1 61 ia4DGMN/Q93okGGIR2EPHnW5ZIw Indeed, cells expressing Psm3-2A (alanine substitution at T182 and S1001) showed mono-orientation defects (20%) albeit mildly, compared with WT (12%) (Fig 3C), suggesting that phosphorylation of these sites might contribute to establishing cohesion at the core cen- tromeres.....We speculate that the Psm3 phosphorylation at the gate may play a role in transiently loosening Rec8-Psm3 gate to facilitate establishment of cohesion at the core centromere. ------- COMMENT: f0575bfe108d54e1 62 Rbd2NEbeZ2ElnmCklZEBvuMxzXs (Figs 3A and S3A–E) We examined if Psm3 is phosphorylated by Plo1 in vitro and found phosphorylation of T182 in the N terminus and S1001 in the C terminus, both locate in the coiled-coil region of the DNA exit gate (Figs 3A and S3A–E), These results suggest that the phosphorylation at Psm3-S110 specifically regulates Rec8 cohesin at centromeres most likely depending on Moa1-Plo1. ------- COMMENT: f0575bfe108d54e1 63 3a+PHBDvoqIhAcvcH1IkEH7Zh3g Indeed, cells expressing Psm3-2A (alanine substitution at T182 and S1001) showed mono-orientation defects (20%) albeit mildly, compared with WT (12%) (Fig 3C), suggesting that phosphorylation of these sites might contribute to establishing cohesion at the core cen- tromeres. ------- COMMENT: f0575bfe108d54e1 65 Wa704ErZbS8JnIG3wofae7P6Xaw Accordingly, rec8-15A psm3-3A double mutant showed more defects in mono-orientation than either rec8-15A or psm3-3A mutant (Fig 4E), suggesting that the phosphorylation on Rec8 and Psm3 cooperatively act to establish cohesion at the core centro- meres. ------- COMMENT: f0575bfe108d54e1 68 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: f0575bfe108d54e1 69 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f0575bfe108d54e1 70 5Lf82Ue1kp90xxOmhbHLTZcTHdw We examined if Psm3 is phosphorylated by Plo1 in vitro and found phosphorylation of T182 in the N terminus and S1001 in the C terminus (Fig. S3) ------- COMMENT: f05a121e4791199c 117 chk6laYbCSaZg3gShSu499mthbA Deletion of this Iec1 protein or the Ino80 complex subunit arp8, ies6, or ies2 causes defects in DNA damage repair, the response to replication stress, and nucleotide metabolism. ------- COMMENT: f05a121e4791199c 118 chk6laYbCSaZg3gShSu499mthbA Deletion of this Iec1 protein or the Ino80 complex subunit arp8, ies6, or ies2 causes defects in DNA damage repair, the response to replication stress, and nucleotide metabolism. ------- COMMENT: f05a121e4791199c 119 chk6laYbCSaZg3gShSu499mthbA Deletion of this Iec1 protein or the Ino80 complex subunit arp8, ies6, or ies2 causes defects in DNA damage repair, the response to replication stress, and nucleotide metabolism. ------- COMMENT: f05a121e4791199c 120 chk6laYbCSaZg3gShSu499mthbA Deletion of this Iec1 protein or the Ino80 complex subunit arp8, ies6, or ies2 causes defects in DNA damage repair, the response to replication stress, and nucleotide metabolism. ------- COMMENT: f05a121e4791199c 122 chk6laYbCSaZg3gShSu499mthbA Deletion of this Iec1 protein or the Ino80 complex subunit arp8, ies6, or ies2 causes defects in DNA damage repair, the response to replication stress, and nucleotide metabolism. ------- COMMENT: f05a121e4791199c 123 chk6laYbCSaZg3gShSu499mthbA Deletion of this Iec1 protein or the Ino80 complex subunit arp8, ies6, or ies2 causes defects in DNA damage repair, the response to replication stress, and nucleotide metabolism. ------- COMMENT: f05a121e4791199c 124 chk6laYbCSaZg3gShSu499mthbA Deletion of this Iec1 protein or the Ino80 complex subunit arp8, ies6, or ies2 causes defects in DNA damage repair, the response to replication stress, and nucleotide metabolism. ------- COMMENT: f05a121e4791199c 125 chk6laYbCSaZg3gShSu499mthbA Deletion of this Iec1 protein or the Ino80 complex subunit arp8, ies6, or ies2 causes defects in DNA damage repair, the response to replication stress, and nucleotide metabolism. ------- COMMENT: f07cbe80a4008b28 5 NhkOKYbCh4Xk5344C0hpf+HgaEU Fig. 1B ------- COMMENT: f07cbe80a4008b28 10 NWblHkZxOQKk0eGX76JHcznMP30 Fig. 2B (comment: CHECK abolished mono orientation at meiosis I) ------- COMMENT: f07cbe80a4008b28 11 WpOlg8E+AxCxrG1L8K9K33ML2tA Fig. 2B (comment: CHECK abolished kinetochore mono orientation at meiosis I) ------- COMMENT: f07cbe80a4008b28 12 0cn96O+PUHoWOuDN0dy+AYGNHtA Fig. 2C ------- COMMENT: f07cbe80a4008b28 13 0cn96O+PUHoWOuDN0dy+AYGNHtA Fig. 2C ------- COMMENT: f07cbe80a4008b28 14 0cn96O+PUHoWOuDN0dy+AYGNHtA Fig. 2C ------- COMMENT: f07cbe80a4008b28 19 StjFsf+0ikEE+/5YTvx754Kz6WY Fig 4A, 4B ------- COMMENT: f07cbe80a4008b28 21 0cn96O+PUHoWOuDN0dy+AYGNHtA Fig. 2C ------- COMMENT: f07cbe80a4008b28 22 hWHkh3R2+dqMFsUvPleqxd5itaQ Fig. 4E (comment: CHECK decreased kinetochore mono orientation at meiosis I) ------- COMMENT: f07cbe80a4008b28 23 hWHkh3R2+dqMFsUvPleqxd5itaQ Fig. 4E (comment: CHECK decreased kinetochore mono orientation at meiosis I) ------- COMMENT: f07cbe80a4008b28 24 hWHkh3R2+dqMFsUvPleqxd5itaQ Fig. 4E (comment: CHECK decreased kinetochore mono orientation at meiosis I) ------- COMMENT: f07cd572b2d0adcf 4 k1+pdIOX8Q8dCeT/v88LUpzcYnQ (comment: blotted for rps601 & rps602 simultaneously) ------- COMMENT: f07cd572b2d0adcf 5 k1+pdIOX8Q8dCeT/v88LUpzcYnQ (comment: blotted for rps601 & rps602 simultaneously) ------- COMMENT: f07cd572b2d0adcf 7 IGLLw/LhgJL71ywRg0FJw2HrWKQ (comment: both rps proteins in extension because blot is for both of them) ------- COMMENT: f07cd572b2d0adcf 8 IGLLw/LhgJL71ywRg0FJw2HrWKQ (comment: both rps proteins in extension because blot is for both of them) ------- COMMENT: f07cd572b2d0adcf 9 IGLLw/LhgJL71ywRg0FJw2HrWKQ (comment: both rps proteins in extension because blot is for both of them) ------- COMMENT: f07cd572b2d0adcf 11 095fkxqDn2wqi4DqKs0YmFJ7Kq0 In this experiment, we utilized a Psk1 mutant (Thr415Glu), a phospho-mimetic mutant of its hydrophobic motif, as the mutant exhibited higher activity than the wild-type protein. As shown in Fig. 2C, the Psk1 protein phosphorylated Rsp6 in vitro. However, the Rsp6 mutant that has two potential serine phosphorylation sites changed to alanine was not phosphorylated by Psk1. ------- COMMENT: f07cd572b2d0adcf 13 Bubqd/+aJTzoqMUqrKgtny1AVG4 (comment: in vitro assay using rps602 so I am inferring rps601) ------- COMMENT: f07cd572b2d0adcf 15 NX6v/toclXh9222PH8RUlVRwEAI not shown ------- COMMENT: f07cd572b2d0adcf 45 IGLLw/LhgJL71ywRg0FJw2HrWKQ (comment: both rps proteins in extension because blot is for both of them) ------- COMMENT: f07cd572b2d0adcf 46 IGLLw/LhgJL71ywRg0FJw2HrWKQ (comment: both rps proteins in extension because blot is for both of them) ------- COMMENT: f07cd572b2d0adcf 47 IGLLw/LhgJL71ywRg0FJw2HrWKQ (comment: both rps proteins in extension because blot is for both of them) ------- COMMENT: f07cd572b2d0adcf 48 IGLLw/LhgJL71ywRg0FJw2HrWKQ (comment: both rps proteins in extension because blot is for both of them) ------- COMMENT: f07cd572b2d0adcf 53 IGLLw/LhgJL71ywRg0FJw2HrWKQ (comment: both rps proteins in extension because blot is for both of them) ------- COMMENT: f07cd572b2d0adcf 54 IGLLw/LhgJL71ywRg0FJw2HrWKQ (comment: both rps proteins in extension because blot is for both of them) ------- COMMENT: f07cd572b2d0adcf 67 KcFjwwlafSLMeKkx4LxtQwMSBZY (comment: I guess everything in the signaling cascade that isn't the "final effector" is part of the signaling cascade?) ------- COMMENT: f07cd572b2d0adcf 68 KcFjwwlafSLMeKkx4LxtQwMSBZY (comment: I guess everything in the signaling cascade that isn't the "final effector" is part of the signaling cascade?) ------- COMMENT: f07cd572b2d0adcf 71 Bubqd/+aJTzoqMUqrKgtny1AVG4 (comment: in vitro assay using rps602 so I am inferring rps601) ------- COMMENT: f08c0f4e1a2f2ba5 19 lnCWmXnTqSyMQZeHQ22q1OHhqNw (comment: substrate: recombinant mono-nucleosomes) ------- COMMENT: f08c0f4e1a2f2ba5 36 WmA4pKbNpQBL4gRTOaH+nqJy8eQ (comment: substrate: bulk histone octamers) ------- COMMENT: f08c0f4e1a2f2ba5 37 PsbQHgoxK2pm0K5WthaPxIRipNI (comment: CHECK not increased (relative to wild type Hht3+/Clr4+) as with hht3-K9M alone) ------- COMMENT: f08c0f4e1a2f2ba5 38 PsbQHgoxK2pm0K5WthaPxIRipNI (comment: CHECK not increased (relative to wild type Hht3+/Clr4+) as with hht3-K9M alone) ------- COMMENT: f08c0f4e1a2f2ba5 73 ljEGYIQR6g6+7/3VrYBgbfwY5AE (comment: Clr4 chromodomain (aa 1-190); histone H3 aa 1-21; interaction strongest with H3-K9me3) ------- COMMENT: f08c0f4e1a2f2ba5 74 ljEGYIQR6g6+7/3VrYBgbfwY5AE (comment: Clr4 chromodomain (aa 1-190); histone H3 aa 1-21; interaction strongest with H3-K9me3) ------- COMMENT: f08c0f4e1a2f2ba5 75 ljEGYIQR6g6+7/3VrYBgbfwY5AE (comment: Clr4 chromodomain (aa 1-190); histone H3 aa 1-21; interaction strongest with H3-K9me3) ------- COMMENT: f08c0f4e1a2f2ba5 76 Kvhx6G3wZWPZ5Ozv3Tjc2yUZp1o (comment: Swi6 chromodomain (aa 1-190); histone H3 aa 1-21; interaction with H3-K9me2 or H3-K9me3) ------- COMMENT: f08c0f4e1a2f2ba5 77 Kvhx6G3wZWPZ5Ozv3Tjc2yUZp1o (comment: Swi6 chromodomain (aa 1-190); histone H3 aa 1-21; interaction with H3-K9me2 or H3-K9me3) ------- COMMENT: f08c0f4e1a2f2ba5 78 Kvhx6G3wZWPZ5Ozv3Tjc2yUZp1o (comment: Swi6 chromodomain (aa 1-190); histone H3 aa 1-21; interaction with H3-K9me2 or H3-K9me3) ------- COMMENT: f08c0f4e1a2f2ba5 79 V+oK8jzxtm/+sMGaB7txuUFQYRA (comment: Chp1 chromodomain (aa 1-190); histone H3 aa 1-21; interaction with H3-K9me2 or H3-K9me3) ------- COMMENT: f08c0f4e1a2f2ba5 80 V+oK8jzxtm/+sMGaB7txuUFQYRA (comment: Chp1 chromodomain (aa 1-190); histone H3 aa 1-21; interaction with H3-K9me2 or H3-K9me3) ------- COMMENT: f08c0f4e1a2f2ba5 81 V+oK8jzxtm/+sMGaB7txuUFQYRA (comment: Chp1 chromodomain (aa 1-190); histone H3 aa 1-21; interaction with H3-K9me2 or H3-K9me3) ------- COMMENT: f08c0f4e1a2f2ba5 82 gvMsvA2o2iu5nvkEBkmUpSYmrXA (comment: Chp2 chromodomain (aa 1-190); histone H3 aa 1-21; interaction with H3-K9me2 or H3-K9me3) ------- COMMENT: f08c0f4e1a2f2ba5 83 gvMsvA2o2iu5nvkEBkmUpSYmrXA (comment: Chp2 chromodomain (aa 1-190); histone H3 aa 1-21; interaction with H3-K9me2 or H3-K9me3) ------- COMMENT: f08c0f4e1a2f2ba5 84 gvMsvA2o2iu5nvkEBkmUpSYmrXA (comment: Chp2 chromodomain (aa 1-190); histone H3 aa 1-21; interaction with H3-K9me2 or H3-K9me3) ------- COMMENT: f0915212069f1a84 1 RDfXok9iSjwVJdisXbUVN5md/6s Fig. 5A. bqt4delta/ telomerase + cells exhibited wild-type telomeres that were stable in post-mitotic cells ------- COMMENT: f0915212069f1a84 3 iSPfivGcsuP+l01m9+7/PS07IeU In contrast to ter1􏰀 cells in which the loss of growth capacity was progressive, the growth of bqt4􏰀 ter1􏰀 cells was severely impaired (Figure 4A and Supplementary Fig- ure S2). ------- COMMENT: f0915212069f1a84 5 etqDIwWDC+v6SImSc48maCAcHrA When ter1+ gene was deleted in bqt4􏰀 cells, we observed that the combination of telomere erosion and NE dissociation provokes a massive accumula- tion of TERRA in Vg cells and this robust increase in tran- scription is even stronger after 48H in quiescence ------- COMMENT: f0915212069f1a84 7 7EOO70rdQ6svNIGyOT+45YxfIgY TERRA level was higher in bqt4􏰀 than WT in vegetative cells and this difference was substantially inten- sified after 48H in quiescence (Figure 6A), ------- COMMENT: f0915212069f1a84 8 JJ61Rr+BzZ+yYkS4ndaMB26Ouxc Fig. 2B ------- COMMENT: f0915212069f1a84 9 EqOxaTp/Y5LMKTa8uwsDiCmTsNg Figure 2D We found that telomere attrition observed in the absence of telomerase did not significantly impair telomere hyperclusterization in quiescence. However, telomere clus- terization did not reach WT level in ter1􏰀 cells after 3 days in G0. ------- COMMENT: f0915212069f1a84 10 MuER3QrMCr8hnt227rkPFb8wGPE Fig. 2D althouh also the percentage of cells that contain a unique telomeric cluster in G0 after streaks 3 and 4 (Fig- ure 2D). We found that telomere attrition observed in the absence of telomerase did not significantly impair telomere hyperclusterization in quiescence. However, telomere clus- terization did not reach WT level in ter1􏰀 cells after 3 days in G0. ------- COMMENT: f0915212069f1a84 11 V7bho4piu8qcG3CklzkbS9fg6M8 Fig. 3D We confirmed that telomere foci moved from nuclear periphery to a more central area (zone 1 to zone 2 or 3) in bqt4􏰀 Vg cells ------- COMMENT: f0915212069f1a84 14 1optHicWQQEI4+ldT9de8DaqBtg zoning of telomere foci within the nuclear envelope was severely impaired in bqt4􏰀 ter1􏰀 for vegetative and qui- escent cells (Supplementary Figure S3D and E). ------- COMMENT: f0915212069f1a84 15 1optHicWQQEI4+ldT9de8DaqBtg zoning of telomere foci within the nuclear envelope was severely impaired in bqt4􏰀 ter1􏰀 for vegetative and qui- escent cells (Supplementary Figure S3D and E). ------- COMMENT: f0915212069f1a84 16 3a4WFh+LltNeOKbgTWRbz+fXiMw STEEx were readily detected as two bands at 1500 and 900 bp, the highest one being prevalent (Figure 5A, right panel). Strikingly, we observed a massive accumulation of STEEx in quiescent bqt4􏰀 ter1􏰀 cells at early time points of quiescence (Figure 5A). ------- COMMENT: f0915212069f1a84 17 7EOO70rdQ6svNIGyOT+45YxfIgY TERRA level was higher in bqt4􏰀 than WT in vegetative cells and this difference was substantially inten- sified after 48H in quiescence (Figure 6A), ------- COMMENT: f0915212069f1a84 18 etqDIwWDC+v6SImSc48maCAcHrA When ter1+ gene was deleted in bqt4􏰀 cells, we observed that the combination of telomere erosion and NE dissociation provokes a massive accumula- tion of TERRA in Vg cells and this robust increase in tran- scription is even stronger after 48H in quiescence ------- COMMENT: f0915212069f1a84 19 CV1TqpPMQUtak9fi+sHqzUJwifg accumulation of TERRA depends on Cid14, a RNA poly adenyl-transferase, (Supplementary Figure S5) ------- COMMENT: f0915212069f1a84 20 jdQyDVkftFyNbqjxwAQlFHksnsc As previously shown, we ob- served that telomere erosion and STEEx formation in ter1􏰀 cells correlates with defects to exit properly from G0 (22) ------- COMMENT: f0915212069f1a84 21 9SeokfVLp/kZJRT9PI5+ZhM8ZgY Indeed, in bqt4􏰀 ter1􏰀 cells the percentage of cells that are unable to form a colony increased in correlation with the massive accumulation of STEEx at D1 and D3 of senes- cence ------- COMMENT: f0afc6fe7b0b439a 1 ucOFnGNOq+EKYZlrU8bcSTVC1gU (comment: Cnd3 depletion following promoter shut-off and auxin-induced degron activation) ------- COMMENT: f0afc6fe7b0b439a 2 ucOFnGNOq+EKYZlrU8bcSTVC1gU (comment: Cnd3 depletion following promoter shut-off and auxin-induced degron activation) ------- COMMENT: f0afc6fe7b0b439a 3 o7m3LtFlr4Y64Pbn2vkW39CRgN8 Supplementary Figs. 1b, 1f, 4a–c (comment: Cnd3 depletion following promoter shut-off and auxin-induced degron activation) ------- COMMENT: f0afc6fe7b0b439a 4 Nbbn5q1AfnswxTrB23rS3mzg75Y (comment: (vw made more specific) Hi-C) Supplementary Figs. 1b, 1f, 4a–c ------- COMMENT: f0afc6fe7b0b439a 5 ViZcDON1VwCMu927nyGfqQeb7gA fig 2b (comment: hi-C difference assay) ------- COMMENT: f0afc6fe7b0b439a 7 vyjx2vI93vGhv3r6XcjJ06D9U6E (comment: hi C????? ) Supplementary Figs. 1b, 1f, 4a–c ------- COMMENT: f0afc6fe7b0b439a 8 hUjQdrZmd7usVO0xCrIQ0JkMBT8 (comment: hic and) Fig. 3C (comment: pcr ) fig 2d increased mitotic intra centromere connection ------- COMMENT: f0afc6fe7b0b439a 9 F3M6ylW5DEM7qJRwjwTF8aJ9zPM fig 3 (comment: hi-C) ------- COMMENT: f0c27afd512b81c3 1 ClBMNNhJyIRijY/mgufIuHrZV2M (comment: Phenotype is greatly enhanced by mutation of the IR-R boundary element) ------- COMMENT: f0d230c2ef37468d 10 cldHXPozhk0zjWsLZJbZkSCAy40 (comment: although this was not assayed it can be deduced from the requirement of both cca1 andd 2 to add CCA) ------- COMMENT: f0d230c2ef37468d 11 cldHXPozhk0zjWsLZJbZkSCAy40 (comment: although this was not assayed it can be deduced from the requirement of both cca1 andd 2 to add CCA) ------- COMMENT: f0d230c2ef37468d 12 cldHXPozhk0zjWsLZJbZkSCAy40 (comment: although this was not assayed it can be deduced from the requirement of both cca1 andd 2 to add CCA) ------- COMMENT: f0d230c2ef37468d 13 cldHXPozhk0zjWsLZJbZkSCAy40 (comment: although this was not assayed it can be deduced from the requirement of both cca1 andd 2 to add CCA) ------- COMMENT: f0f3b1ee8e5bd5bf 3 4Q2sNTPcp11MAq+OJI/f43GjmMA (comment: CHECK H3 K9me3 https://github.com/geneontology/go-ontology/issues/16331) ------- COMMENT: f0f3b1ee8e5bd5bf 4 UuMjrR96ioImkzl/JTZhZi1+uCA (comment: CHECK H3 K9me3) ------- COMMENT: f0f3b1ee8e5bd5bf 8 4Q2sNTPcp11MAq+OJI/f43GjmMA (comment: CHECK H3 K9me3 https://github.com/geneontology/go-ontology/issues/16331) ------- COMMENT: f0f3b1ee8e5bd5bf 9 4Q2sNTPcp11MAq+OJI/f43GjmMA (comment: CHECK H3 K9me3 https://github.com/geneontology/go-ontology/issues/16331) ------- COMMENT: f134593392c70d97 27 zDiZf/+AxjxNN813Ju2MIT/9GhU (comment: Yeast two hybrid) ------- COMMENT: f14b30da9b0b94c4 80 5+pKTo1xKId5HF/vXg+uvBIhdD8 (comment: CHECK serine 2) ------- COMMENT: f15fd8abedae83c7 2 rtvZs7nfnvQ/ZpEmTvrqq1/dllg In Msp1p overexpressing cells, more than 85% of the cells had an aggregated filamentous mitochondrial network. ------- COMMENT: f15fd8abedae83c7 3 QY16tROoWgI9Dd5QL2XfX+ojAPA fragmented: By 27 h, when repression was almost complete, the mitochondrial network fragmented into clusters of small rounded mitochondria. This phenotype is reminiscent of the mitochondrial morphology defect observed in S. cerevisiae deleted for MGM1 [18]. ------- COMMENT: f15fd8abedae83c7 5 BjkmHCQlwAdtbepMAaKzMBs9HG0 while cells that expressed cytosolic Msp1pDMIS or CAT died. ------- COMMENT: f15fd8abedae83c7 6 niX/+HFbWgHBGtj3SrW4rd9Zdjs GTPase (Msp1pK276A) and coiledcoil deleted (Msp1pD25-D50) mutants did not support the function of Msp1p as they failed to complement the msp1+ gene deletion. ------- COMMENT: f15fd8abedae83c7 7 BjkmHCQlwAdtbepMAaKzMBs9HG0 while cells that expressed cytosolic Msp1pDMIS or CAT died. ------- COMMENT: f15fd8abedae83c7 8 BjkmHCQlwAdtbepMAaKzMBs9HG0 while cells that expressed cytosolic Msp1pDMIS or CAT died. ------- COMMENT: f15fd8abedae83c7 9 BjkmHCQlwAdtbepMAaKzMBs9HG0 while cells that expressed cytosolic Msp1pDMIS or CAT died. ------- COMMENT: f15fd8abedae83c7 10 z4vxNgmnXB1FERjB3OhYBq/o5Ng On the contrary, even slight overexpression of Msp1pK276A, Msp1pD50 or Msp1pD25 induced mitochondrial fragmentation; in about 60% of the cells the mitochondria appeared as small more or less clustered individual dots. ------- COMMENT: f15fd8abedae83c7 11 z4vxNgmnXB1FERjB3OhYBq/o5Ng On the contrary, even slight overexpression of Msp1pK276A, Msp1pD50 or Msp1pD25 induced mitochondrial fragmentation; in about 60% of the cells the mitochondria appeared as small more or less clustered individual dots. ------- COMMENT: f15fd8abedae83c7 12 z4vxNgmnXB1FERjB3OhYBq/o5Ng On the contrary, even slight overexpression of Msp1pK276A, Msp1pD50 or Msp1pD25 induced mitochondrial fragmentation; in about 60% of the cells the mitochondria appeared as small more or less clustered individual dots. ------- COMMENT: f15fd8abedae83c7 13 5WxwzcYavF2qHHCk/lG1e8RrLWA Time-course measurements of the doubling times of these cultures showed that at day 5 the growth rate of strains expressing Msp1pK276A, Msp1pD50 and Msp1pD25 was greatly increased... (Fig. 4A). ------- COMMENT: f15fd8abedae83c7 14 5WxwzcYavF2qHHCk/lG1e8RrLWA Time-course measurements of the doubling times of these cultures showed that at day 5 the growth rate of strains expressing Msp1pK276A, Msp1pD50 and Msp1pD25 was greatly increased... (Fig. 4A). ------- COMMENT: f15fd8abedae83c7 15 5WxwzcYavF2qHHCk/lG1e8RrLWA Time-course measurements of the doubling times of these cultures showed that at day 5 the growth rate of strains expressing Msp1pK276A, Msp1pD50 and Msp1pD25 was greatly increased... (Fig. 4A). ------- COMMENT: f15fd8abedae83c7 16 CSG75SEqb007xkRFeqojQcbLsNQ (Fig. 4B), Loss of the GTPase function of Msp1p is thus sufficient to affect the maintenance of the mitochondrial genome and the viability of S. pombe cells. ------- COMMENT: f15fd8abedae83c7 17 4fqOgi6Fvl9yo9pP1g0M9Q5HmyM Fig. 4B), ------- COMMENT: f15fd8abedae83c7 18 4fqOgi6Fvl9yo9pP1g0M9Q5HmyM Fig. 4B), ------- COMMENT: f15fd8abedae83c7 19 5ntsmwyj+Tw77omujfhfVGRm+pI the mitochondrial network appeared as highly interconnected tubules forming net-like structures (Fig. 5A). ------- COMMENT: f15fd8abedae83c7 20 Py7NwU4RChk58xBc4ODarKL4gmk In the doubledisrupted Dmsp1Ddnm1 strain, the mitochondria formed elongated tubules which resembled those seen in wild-type cells, ...... (Fig. 5E). ------- COMMENT: f15fd8abedae83c7 23 Owo8/tAVzE8LIUH056qbxHQ4bcE In the Dmsp1Ddnm1 strain, mtDNA depletion (Fig. 5F) and lethality (not shown) did not occur. ------- COMMENT: f15fd8abedae83c7 25 SlDHiMRMY2bnViVMqTNxAYs8/XM In the Dmsp1Ddnm1 strain, mtDNA depletion (Fig. 5F) and lethality (not shown) did not occur ------- COMMENT: f15fd8abedae83c7 26 fmqzo1O+yjnstjgflP9KrkcOD9w (comment: MEMBRANE) ------- COMMENT: f1732559a5f79ef5 90 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: f17c3d43d258f9f6 2 MYFFj9eR6fLcJSIB3KhUEm8jmxs Red1 localizes to cleavage bodies in mitotically dividing cells and cooperates with polyadenylation factors to hyperadenylate meiotic mRNAs. (A) Red1 dots co-localize with cleavage factor, Pcf11; the canonical poly(A) polymerase, Pla1; a nuclear exosome subunit, Rrp6; and a nuclear poly(A)-binding protein, Pab2. ------- COMMENT: f17c3d43d258f9f6 20 U5Sj2jhr3Pli+LgfGvdqhBoi7gw (comment: poly(A) tails longer in rrp6delta alone, but wild type not shown for meiotic cell cycle so can't annotate rrp6delta phenotype as normal or increased length) ------- COMMENT: f17c3d43d258f9f6 28 MYFFj9eR6fLcJSIB3KhUEm8jmxs Red1 localizes to cleavage bodies in mitotically dividing cells and cooperates with polyadenylation factors to hyperadenylate meiotic mRNAs. (A) Red1 dots co-localize with cleavage factor, Pcf11; the canonical poly(A) polymerase, Pla1; a nuclear exosome subunit, Rrp6; and a nuclear poly(A)-binding protein, Pab2. ------- COMMENT: f17c3d43d258f9f6 29 MYFFj9eR6fLcJSIB3KhUEm8jmxs Red1 localizes to cleavage bodies in mitotically dividing cells and cooperates with polyadenylation factors to hyperadenylate meiotic mRNAs. (A) Red1 dots co-localize with cleavage factor, Pcf11; the canonical poly(A) polymerase, Pla1; a nuclear exosome subunit, Rrp6; and a nuclear poly(A)-binding protein, Pab2. ------- COMMENT: f17c3d43d258f9f6 30 MYFFj9eR6fLcJSIB3KhUEm8jmxs Red1 localizes to cleavage bodies in mitotically dividing cells and cooperates with polyadenylation factors to hyperadenylate meiotic mRNAs. (A) Red1 dots co-localize with cleavage factor, Pcf11; the canonical poly(A) polymerase, Pla1; a nuclear exosome subunit, Rrp6; and a nuclear poly(A)-binding protein, Pab2. ------- COMMENT: f17c3d43d258f9f6 31 MYFFj9eR6fLcJSIB3KhUEm8jmxs Red1 localizes to cleavage bodies in mitotically dividing cells and cooperates with polyadenylation factors to hyperadenylate meiotic mRNAs. (A) Red1 dots co-localize with cleavage factor, Pcf11; the canonical poly(A) polymerase, Pla1; a nuclear exosome subunit, Rrp6; and a nuclear poly(A)-binding protein, Pab2. ------- COMMENT: f1c2dba49bdde890 1 fHaj7aQHWAOiUjUnPkEad74QHmg increase with telomere shortening and time in quiescence ------- COMMENT: f1c2dba49bdde890 14 fHaj7aQHWAOiUjUnPkEad74QHmg increase with telomere shortening and time in quiescence ------- COMMENT: f1c2dba49bdde890 15 NHmD3RWsmjrVExGtaDNSIpgdIwo limit subtelomeric DNA amplification in G0 ------- COMMENT: f1c2dba49bdde890 17 RYkV8O41qXW5eqdUqzuRilqBRXw Require for subtelomeric DNA amplification in G0 ------- COMMENT: f1c2dba49bdde890 18 s1cBJn6xpVzV/DmmsEZ7wIDRfuM require for subtelomeric DNA amplification in G0 ------- COMMENT: f1cc03a9de929ac1 1 77BX97Fy/WpQshPpHe6LX4ohkq4 (Fig. 1A,1 B) ------- COMMENT: f1cc03a9de929ac1 2 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: f1cc03a9de929ac1 3 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: f1cc03a9de929ac1 4 p3x2dDTjQlS9jbO+IMHpHbED4nw (Fig. 1D) ------- COMMENT: f1cc03a9de929ac1 5 p3x2dDTjQlS9jbO+IMHpHbED4nw (Fig. 1D) ------- COMMENT: f1cc03a9de929ac1 6 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: f1cc03a9de929ac1 7 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: f1cc03a9de929ac1 8 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: f1cc03a9de929ac1 9 XCWlyliLIVMVpRmvvapufdD+598 (Fig. 2C) ------- COMMENT: f1cc03a9de929ac1 10 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: f1cc03a9de929ac1 11 d2NdxnatvYWNnh9afhcOiSOs0XA (Fig. S1D) ------- COMMENT: f1cc03a9de929ac1 12 xLVWFjf4bQYJtIPzamDpIL6W7oM (Fig. 2E) ------- COMMENT: f1cc03a9de929ac1 13 WcU1y+t4I3/E2fLOax3AIJXZNn4 (Fig. 2F) ------- COMMENT: f1cc03a9de929ac1 14 WcU1y+t4I3/E2fLOax3AIJXZNn4 (Fig. 2F) ------- COMMENT: f1cc03a9de929ac1 15 DES5Jkiy+rZ7wehRYgobQ94L1Q8 (Fig. 2G) ------- COMMENT: f1cc03a9de929ac1 16 DpgZxM1Qv/toBJXudZJgJhXmBuA (Fig. 3A) ------- COMMENT: f1cc03a9de929ac1 17 VM5mMuN0DSUkFO7rMItwoFnEQjw (Fig. 3B) ------- COMMENT: f1cc03a9de929ac1 18 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: f1cc03a9de929ac1 19 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: f1cc03a9de929ac1 20 9gZprW9BzLRWef/9zh1LRlQEA8Y (Fig. 4C) ------- COMMENT: f1cc03a9de929ac1 21 GkI93EYSqP6NybAtsJjxO2fy3Ic (Fig. 4D) ------- COMMENT: f1cc03a9de929ac1 22 MfhXrratyk9HEpPvYNKyPqJLEoI (Fig. 4E) ------- COMMENT: f1cc03a9de929ac1 23 siBQpTiZN7gnmW8WAC4wWEDRT80 (Fig. 5D) ------- COMMENT: f1cc03a9de929ac1 24 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: f1cc03a9de929ac1 25 hPFNK9pXn9s6uyKJPTkg3DIM2XE (Fig. 6A) ------- COMMENT: f1cc03a9de929ac1 26 sUCS79Rs9xFD1wViTRlsgeGdNFs (Fig. 6B) ------- COMMENT: f1cc03a9de929ac1 27 wnx2gS3IAKqzPkStlyJ0uF9GveM (Fig. 6C) ------- COMMENT: f1cc03a9de929ac1 28 na4Qp0iKCYpqF/XnQD43esdEOlY (Fig. 7A) ------- COMMENT: f1cc03a9de929ac1 29 vGtIAlSSPNnMrmPTCV9WpCBeLHE (Fig. 7B) ------- COMMENT: f1cc03a9de929ac1 30 kb7NOBRiqlmzY3vQrPFeR5NkglI (Fig. 7C) ------- COMMENT: f20d1a8914693c8e 1 hpLB+v3pTRFj2Lnb+/rH1ObnayI This reverse genetic approach identified a strain bearing a deletion in the annotated open reading frame, SPCC1235.09, which encodes a WD repeat domain protein (Figure 1). ------- COMMENT: f20d1a8914693c8e 2 eUkwlZ3xqzhyl6ZKYaD8oxmQdho figure 2c ------- COMMENT: f20d1a8914693c8e 3 FKxUsvuuY/26e7h2qLzZwvVYlS8 Consistent with a role in chromatin modification, all three proteins localized to the nucleus (Figure 4A). ------- COMMENT: f20d1a8914693c8e 4 FKxUsvuuY/26e7h2qLzZwvVYlS8 Consistent with a role in chromatin modification, all three proteins localized to the nucleus (Figure 4A). ------- COMMENT: f20d1a8914693c8e 5 FKxUsvuuY/26e7h2qLzZwvVYlS8 Consistent with a role in chromatin modification, all three proteins localized to the nucleus (Figure 4A). ------- COMMENT: f20d1a8914693c8e 6 Nfe0L6hfzXDgxwLs4JIPfyrMH6g Taken together, these data demonstrate that Hif2p, Set3p, and Snt1p exist as part of a nuclear-localized protein complex in S. pombe. ------- COMMENT: f20d1a8914693c8e 7 Nfe0L6hfzXDgxwLs4JIPfyrMH6g Taken together, these data demonstrate that Hif2p, Set3p, and Snt1p exist as part of a nuclear-localized protein complex in S. pombe. ------- COMMENT: f20d1a8914693c8e 8 Nfe0L6hfzXDgxwLs4JIPfyrMH6g Taken together, these data demonstrate that Hif2p, Set3p, and Snt1p exist as part of a nuclear-localized protein complex in S. pombe. ------- COMMENT: f2155bc676f8eae1 1 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: f2155bc676f8eae1 2 N7pwJaOn1adI2t85iv3FFq+115g Figure 1 about 14% from new end In contrast, 14% of tea1  cells and 30% of pom1  cells failed to resume growth from a previously growing end (Figure 1). ------- COMMENT: f2155bc676f8eae1 3 AFjGN3uHrto9NeRd6qvyoG0hlRY Figure 1 about 30% from new end. In contrast, 14% of tea1  cells and 30% of pom1  cells failed to resume growth from a previously growing end (Figure 1). ------- COMMENT: f2155bc676f8eae1 4 Txqe9L3/GDcGkap7pWPn28PgluU Figure 1 (comment: about 30% from old end) ------- COMMENT: f2155bc676f8eae1 5 3nousnyA3EWXw6W/ondEdhrnCL8 Figure 1 (comment: about 60% from old end) ------- COMMENT: f2155bc676f8eae1 6 3nousnyA3EWXw6W/ondEdhrnCL8 Figure 1 (comment: about 60% from old end) ------- COMMENT: f2155bc676f8eae1 7 RhcilEm+NSMVFn+Dvj9U0T0Wo6c Figure 1 Tea3  cells resumed growth from their old end after division, indicating that the cell inheriting a growing end had no difficulty in reidentifying it as the appropriate site for growth after division (Figure 1). ------- COMMENT: f2155bc676f8eae1 8 oyF19C01jqs+FZrk1KOwtmVoSZM Figure 3 Our tea1  result differs from that of Rupes et al. (1999), who found that tea1  cells did not switch to bipolar growth after a LatA pulse. This may be due to differences in scoring or temperature between the experiments. ------- COMMENT: f2155bc676f8eae1 9 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: f2155bc676f8eae1 10 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: f2155bc676f8eae1 11 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: f2155bc676f8eae1 12 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: f2155bc676f8eae1 13 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: f2155bc676f8eae1 14 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: f2155bc676f8eae1 15 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: f2155bc676f8eae1 20 pEaY9+nVYcMF8xcnkn1VrqqPj/c Figure 4 shows that tea1  cells at high temperatures displayed microtubules bending round the cell ends, in accordance with previously published results (Mata and Nurse, 1997). ------- COMMENT: f2155bc676f8eae1 21 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: f2155bc676f8eae1 22 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: f2155bc676f8eae1 23 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: f2155bc676f8eae1 24 bXmPw2anYkWQh87P4JAKMo9p6zA figure5 ------- COMMENT: f2155bc676f8eae1 25 bXmPw2anYkWQh87P4JAKMo9p6zA figure5 ------- COMMENT: f2155bc676f8eae1 26 bXmPw2anYkWQh87P4JAKMo9p6zA figure5 ------- COMMENT: f2155bc676f8eae1 27 bXmPw2anYkWQh87P4JAKMo9p6zA figure5 ------- COMMENT: f2155bc676f8eae1 28 bXmPw2anYkWQh87P4JAKMo9p6zA figure5 ------- COMMENT: f21f4f173822b0e2 1 l8vomPCDc9KlnNw6IEHKiG0CYmg Deletion increases levels of mitotic checkpoint complex associated with the anaphase promoting complex in mitosis. ------- COMMENT: f21f4f173822b0e2 2 +HO1lglJbJ4+3QkolTPNZ13XZT4 Required for mitotic checkpoint complex binding to the anaphase promoting complex. ------- COMMENT: f21f4f173822b0e2 3 5UV6PN2bPVnGWux0VLAck3aEmWA (comment: vw I changed the genotype/) Fig 1A (comment: checkpoint assay) ------- COMMENT: f21f4f173822b0e2 4 fHxzj4CNL+5awSJvJNpKk3j8SVw (comment: vw; I changed the genotype here) ------- COMMENT: f21f4f173822b0e2 5 1HI+KYfgtl3iEaGVlqGeeix1UUM (comment: vw changed term from "reduced ubiquitin ligase activity") ------- COMMENT: f21f4f173822b0e2 7 Ax5ogOoEuSKpQnTZSpCnomWVx50 Fig 1A (comment: checkpoint assay) ------- COMMENT: f21f4f173822b0e2 8 Ax5ogOoEuSKpQnTZSpCnomWVx50 Fig 1A (comment: checkpoint assay) ------- COMMENT: f21f4f173822b0e2 9 Ax5ogOoEuSKpQnTZSpCnomWVx50 Fig 1A (comment: checkpoint assay) ------- COMMENT: f21f4f173822b0e2 10 Ax5ogOoEuSKpQnTZSpCnomWVx50 Fig 1A (comment: checkpoint assay) ------- COMMENT: f21f4f173822b0e2 11 Ax5ogOoEuSKpQnTZSpCnomWVx50 Fig 1A (comment: checkpoint assay) ------- COMMENT: f21f4f173822b0e2 12 XPnM32shLX68Eo2rR66Yrm1uKgQ fig 1c ------- COMMENT: f21f4f173822b0e2 13 XPnM32shLX68Eo2rR66Yrm1uKgQ fig 1c ------- COMMENT: f21f4f173822b0e2 14 L7aMz8iqu8tXBfnvmHVYoMAXkW4 fig S1 ------- COMMENT: f21f4f173822b0e2 15 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: f21f4f173822b0e2 16 XPnM32shLX68Eo2rR66Yrm1uKgQ fig 1c ------- COMMENT: f21f4f173822b0e2 17 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: f21f4f173822b0e2 18 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: f21f4f173822b0e2 19 yeSYRHKmUvWuWEHTxMv1A4+Pnik fig 2a ------- COMMENT: f21f4f173822b0e2 20 w0wKkAH6w7GsNsCMiOGd8acmq1c figure 2b ------- COMMENT: f21f4f173822b0e2 21 4QSyRLn416H6FtF6MafRd4eO/P4 Figures 3A, 3B ------- COMMENT: f21f4f173822b0e2 24 cmJlIXt0bCw6rkBS4+210D3Syhc (Figure 3B). ------- COMMENT: f21f4f173822b0e2 25 vCJeZfASHAUeKoi61goSkLRL2Fo (Figure 3A). ------- COMMENT: f21f4f173822b0e2 27 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: f21f4f173822b0e2 28 vCJeZfASHAUeKoi61goSkLRL2Fo (Figure 3A). ------- COMMENT: f21f4f173822b0e2 29 cmJlIXt0bCw6rkBS4+210D3Syhc (Figure 3B). ------- COMMENT: f23547b9f5114991 1 LTCc6QxUmhKkdYinRocxWnxrUac (comment: structure) ------- COMMENT: f23dc3453af59e3b 1 wcV6+xNGJ9trqzsjpslYOIBtgOI (comment: MBP substrate),(comment: CHECK activated_by(CHEBI:29035)) ------- COMMENT: f284fe43c381666f 11 s0tHQPrRLXc6Js1w/+4n9P1ESx4 (comment: also inferred from orthology to all other Orc1s in the world) ------- COMMENT: f29d798df96c977e 1 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: f29d798df96c977e 2 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: f29d798df96c977e 3 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: f29d798df96c977e 4 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: f29d798df96c977e 5 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: f29d798df96c977e 6 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: f29d798df96c977e 7 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: f29d798df96c977e 8 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: f29d798df96c977e 9 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: f29d798df96c977e 10 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: f2b0ffda72370b22 1 ioszzqrwH8fXXIWFu/dVuOHMPJ4 (comment: used endogenous tea2 gene tagged at C term with GFP). Fig1A ------- COMMENT: f2b0ffda72370b22 3 UA6bIlVmFRkbBCi//EphpSviH+4 (comment: used endogenous tea1 gene tagged at C term with YFP and tubulin CFP for live cell imaging of tea1 on microtubules) Fig1C ------- COMMENT: f2b0ffda72370b22 4 ioszzqrwH8fXXIWFu/dVuOHMPJ4 (comment: used endogenous tea2 gene tagged at C term with GFP). Fig1A ------- COMMENT: f2b0ffda72370b22 5 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: f2b0ffda72370b22 6 2PeZ4yjoUCCdoe6qRGJfho6Es8Q Fig2C (comment: OUTSTANDING Q IS IT ALONG OR ON?) ------- COMMENT: f2b0ffda72370b22 9 hKWCsgQ9BP4CeUjS4JB+UtD3J0c Fig3C ------- COMMENT: f2b0ffda72370b22 12 hKWCsgQ9BP4CeUjS4JB+UtD3J0c Fig3C ------- COMMENT: f2b0ffda72370b22 13 hKWCsgQ9BP4CeUjS4JB+UtD3J0c Fig3C ------- COMMENT: f2b0ffda72370b22 14 hKWCsgQ9BP4CeUjS4JB+UtD3J0c Fig3C ------- COMMENT: f2b0ffda72370b22 15 hKWCsgQ9BP4CeUjS4JB+UtD3J0c Fig3C ------- COMMENT: f2b0ffda72370b22 18 PXqQ8w7WZKoenOmS6L1/H8ouUb4 Fig4A ------- COMMENT: f2b0ffda72370b22 19 /XeyewV3vBbG6kQyaKYHlAFfkjw Fig4B. (comment: I know that the protein is localising to the plus end but they did not say this in this paper although they do say it is on the tips of polymerizing microtubules so it could be FYPO 0004731) ------- COMMENT: f2b0ffda72370b22 20 O5ChQshWo9SMl9ef8VwaxdF3zLk Fig4B ------- COMMENT: f2b0ffda72370b22 21 cUd1Xrq7+BFnkFtpYJEH832Zig8 Fig3C, Fig5A (comment: CHECK STILL TO ADD curved around cell end during mitotic interphase) ------- COMMENT: f2b0ffda72370b22 22 s8zxygB4j9+cKrsniSSlYhY+9P0 Fig5A ------- COMMENT: f2b0ffda72370b22 23 s8zxygB4j9+cKrsniSSlYhY+9P0 Fig5A ------- COMMENT: f2b0ffda72370b22 24 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: f2b0ffda72370b22 25 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: f2b0ffda72370b22 26 DPUOKAI+ppqkL6Eh0M85JFQAdbo Fig5B ------- COMMENT: f2b0ffda72370b22 27 3V3HaMQsZES9F/qR1JrWhOrHbFU Fig6 ------- COMMENT: f2b0ffda72370b22 28 3V3HaMQsZES9F/qR1JrWhOrHbFU Fig6 ------- COMMENT: f2b0ffda72370b22 29 3V3HaMQsZES9F/qR1JrWhOrHbFU Fig6 ------- COMMENT: f2debdcbb3120b09 1 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 2 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 3 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 4 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 5 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 6 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 7 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 8 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 9 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 10 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 11 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 12 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 13 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 14 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 15 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 16 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 17 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 18 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 19 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 20 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 21 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 22 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 23 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 24 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 25 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 26 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 27 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 28 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 29 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 30 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 31 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 32 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 33 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 34 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 35 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 36 ba518wRZJluu/jqN+SQEqboKnXY 18 proteins were localized at the centromere throughout the mitotic cell cycle (Table 2; group 1) ------- COMMENT: f2debdcbb3120b09 37 wgmnBbVuLdTa6+wQu4JSvhS7yXg In contrast, four proteins (Dam1, Dad2, Ask1, and Spc34) were localized at the centromere only at the M phase (Table 2; group 2). ------- COMMENT: f2debdcbb3120b09 38 wgmnBbVuLdTa6+wQu4JSvhS7yXg In contrast, four proteins (Dam1, Dad2, Ask1, and Spc34) were localized at the centromere only at the M phase (Table 2; group 2). ------- COMMENT: f2debdcbb3120b09 39 wgmnBbVuLdTa6+wQu4JSvhS7yXg In contrast, four proteins (Dam1, Dad2, Ask1, and Spc34) were localized at the centromere only at the M phase (Table 2; group 2). ------- COMMENT: f2debdcbb3120b09 40 wgmnBbVuLdTa6+wQu4JSvhS7yXg In contrast, four proteins (Dam1, Dad2, Ask1, and Spc34) were localized at the centromere only at the M phase (Table 2; group 2). ------- COMMENT: f2debdcbb3120b09 41 S7l8HZ4TRtb4y0vT9iHJxISTuGA These 4 proteins share homology with the S. cerevisiae DASH com- plex, DAM1, DAD2, ASK1, and SPC34 (Miranda et al., 2005), and the observation that their centromere localization is limited to the M phase has been previously reported in S. pombe (Liu et al., 2005). Thus, we assigned these four proteins to the DASH complex (Table 2) ------- COMMENT: f2debdcbb3120b09 43 S7l8HZ4TRtb4y0vT9iHJxISTuGA These 4 proteins share homology with the S. cerevisiae DASH com- plex, DAM1, DAD2, ASK1, and SPC34 (Miranda et al., 2005), and the observation that their centromere localization is limited to the M phase has been previously reported in S. pombe (Liu et al., 2005). Thus, we assigned these four proteins to the DASH complex (Table 2) ------- COMMENT: f2debdcbb3120b09 44 S7l8HZ4TRtb4y0vT9iHJxISTuGA These 4 proteins share homology with the S. cerevisiae DASH com- plex, DAM1, DAD2, ASK1, and SPC34 (Miranda et al., 2005), and the observation that their centromere localization is limited to the M phase has been previously reported in S. pombe (Liu et al., 2005). Thus, we assigned these four proteins to the DASH complex (Table 2) ------- COMMENT: f2debdcbb3120b09 45 S7l8HZ4TRtb4y0vT9iHJxISTuGA These 4 proteins share homology with the S. cerevisiae DASH com- plex, DAM1, DAD2, ASK1, and SPC34 (Miranda et al., 2005), and the observation that their centromere localization is limited to the M phase has been previously reported in S. pombe (Liu et al., 2005). Thus, we assigned these four proteins to the DASH complex (Table 2) ------- COMMENT: f2debdcbb3120b09 46 l6uxztmFnagJbq38JDGWvsJ+58A Proteins of the Mis6-like group remained at the centromere throughout meiosis (Figure 2B), whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 47 l6uxztmFnagJbq38JDGWvsJ+58A Proteins of the Mis6-like group remained at the centromere throughout meiosis (Figure 2B), whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 48 l6uxztmFnagJbq38JDGWvsJ+58A Proteins of the Mis6-like group remained at the centromere throughout meiosis (Figure 2B), whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 49 l6uxztmFnagJbq38JDGWvsJ+58A Proteins of the Mis6-like group remained at the centromere throughout meiosis (Figure 2B), whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 50 l6uxztmFnagJbq38JDGWvsJ+58A Proteins of the Mis6-like group remained at the centromere throughout meiosis (Figure 2B), whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 51 l6uxztmFnagJbq38JDGWvsJ+58A Proteins of the Mis6-like group remained at the centromere throughout meiosis (Figure 2B), whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 52 l6uxztmFnagJbq38JDGWvsJ+58A Proteins of the Mis6-like group remained at the centromere throughout meiosis (Figure 2B), whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 53 l6uxztmFnagJbq38JDGWvsJ+58A Proteins of the Mis6-like group remained at the centromere throughout meiosis (Figure 2B), whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 54 l6uxztmFnagJbq38JDGWvsJ+58A Proteins of the Mis6-like group remained at the centromere throughout meiosis (Figure 2B), whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 55 l6uxztmFnagJbq38JDGWvsJ+58A Proteins of the Mis6-like group remained at the centromere throughout meiosis (Figure 2B), whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 56 l6uxztmFnagJbq38JDGWvsJ+58A Proteins of the Mis6-like group remained at the centromere throughout meiosis (Figure 2B), whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 57 l6uxztmFnagJbq38JDGWvsJ+58A Proteins of the Mis6-like group remained at the centromere throughout meiosis (Figure 2B), whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 58 l6uxztmFnagJbq38JDGWvsJ+58A Proteins of the Mis6-like group remained at the centromere throughout meiosis (Figure 2B), whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 59 l6uxztmFnagJbq38JDGWvsJ+58A Proteins of the Mis6-like group remained at the centromere throughout meiosis (Figure 2B), whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 60 l6uxztmFnagJbq38JDGWvsJ+58A Proteins of the Mis6-like group remained at the centromere throughout meiosis (Figure 2B), whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 61 l6uxztmFnagJbq38JDGWvsJ+58A Proteins of the Mis6-like group remained at the centromere throughout meiosis (Figure 2B), whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 62 l6uxztmFnagJbq38JDGWvsJ+58A Proteins of the Mis6-like group remained at the centromere throughout meiosis (Figure 2B), whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 63 l6uxztmFnagJbq38JDGWvsJ+58A Proteins of the Mis6-like group remained at the centromere throughout meiosis (Figure 2B), whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 64 KHIQswvLr14feI2dZ3A2/ReL03o whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 65 KHIQswvLr14feI2dZ3A2/ReL03o whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 66 KHIQswvLr14feI2dZ3A2/ReL03o whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 67 KHIQswvLr14feI2dZ3A2/ReL03o whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 68 KHIQswvLr14feI2dZ3A2/ReL03o whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 69 KHIQswvLr14feI2dZ3A2/ReL03o whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 70 KHIQswvLr14feI2dZ3A2/ReL03o whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 71 KHIQswvLr14feI2dZ3A2/ReL03o whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 72 KHIQswvLr14feI2dZ3A2/ReL03o whereas those of the NMS group disappeared from the centromere or their presence was significantly reduced, dur- ing meiotic prophase (Figure 3). ------- COMMENT: f2debdcbb3120b09 73 cVzRNd52Ihg9yMRwJOyQLAKztDE In addition, their centromere localization depended on Mis6: Cnl2 and Fta7 proteins lost their centromere localization in a mis6-302 tem- perature-sensitive mutant at the restricted temperature of 36°C (Figure 1E) ------- COMMENT: f2debdcbb3120b09 74 cVzRNd52Ihg9yMRwJOyQLAKztDE In addition, their centromere localization depended on Mis6: Cnl2 and Fta7 proteins lost their centromere localization in a mis6-302 tem- perature-sensitive mutant at the restricted temperature of 36°C (Figure 1E) ------- COMMENT: f2debdcbb3120b09 75 ry1NEiwWVfBtL6SDoIaAbeizt8w The DASH complex proteins (Dam1, Spc34, Dad2, and Ask1) were not detected during meiotic prophase. They reappeared at the centromere shortly before metaphase of meiosis I (Figure 4), ------- COMMENT: f2debdcbb3120b09 76 ry1NEiwWVfBtL6SDoIaAbeizt8w The DASH complex proteins (Dam1, Spc34, Dad2, and Ask1) were not detected during meiotic prophase. They reappeared at the centromere shortly before metaphase of meiosis I (Figure 4), ------- COMMENT: f2debdcbb3120b09 77 ry1NEiwWVfBtL6SDoIaAbeizt8w The DASH complex proteins (Dam1, Spc34, Dad2, and Ask1) were not detected during meiotic prophase. They reappeared at the centromere shortly before metaphase of meiosis I (Figure 4), ------- COMMENT: f2debdcbb3120b09 78 ry1NEiwWVfBtL6SDoIaAbeizt8w The DASH complex proteins (Dam1, Spc34, Dad2, and Ask1) were not detected during meiotic prophase. They reappeared at the centromere shortly before metaphase of meiosis I (Figure 4), ------- COMMENT: f2debdcbb3120b09 79 XIaMAOVSiTrA34IqHnz7yx3fZS4 Sgo1 protein signal intensity increased in two steps (52 and 20 min before the metaphase–anaphase transition of meiosis I) in a way similar to the NMS (Ndc80- Mis12-Spc7) complex proteins (Figure 7B). ------- COMMENT: f2debdcbb3120b09 80 XIaMAOVSiTrA34IqHnz7yx3fZS4 Sgo1 protein signal intensity increased in two steps (52 and 20 min before the metaphase–anaphase transition of meiosis I) in a way similar to the NMS (Ndc80- Mis12-Spc7) complex proteins (Figure 7B). ------- COMMENT: f2dfea149f8db02b 1 b9ZXUQ86IKoRxDNcphrX1+DmJy4 (comment: *******is this mitotic/meiotic or both) ------- COMMENT: f2e1d2aee3b4e851 44 0Ho/OKO2pswMTC47L4i/cXQ9ehU (comment: CHECK SO:0001899 = dh repeat) ------- COMMENT: f30149c5fcc7f553 1 D7/yARE8aupXaAhlX1hen1+NVEw Figures 1B and S1A Dma1-mNeonGreen became enriched at SPBs prior to SPB separation. It appeared to transiently leave SPBs during anaphase B, returning before telophase and then leaving again after cell division. Dma1 SPB transient loss happens before the development of Cdc7 SPB localiztion asymmetry. Dma1 failed to return to SPBs in late anaphase in cdc7-24 cells at restrictive temperature. Dma1 could be detected on majority SPBs in cdc16-116 cells at restrictive temperature, suggesting high SIN activity promote Dma1 SPB re-accumulation at the end of anaphase. ------- COMMENT: f30149c5fcc7f553 3 BLldJPVjQyYi/PrTWA9P+jmuq8I Figure 1B and S1B Dma1-mNeonGreen forms a ring at cell division site during early mitosis. Then it leaves and returns to cell division site during mitosis. Dma1 transiently leaves cell division site before Sid2 appears at the cell division site. ------- COMMENT: f30149c5fcc7f553 4 JPPtvj2tVc3DBx3Qma7SQbqAkMc Figure 1B and S1B ------- COMMENT: f30149c5fcc7f553 5 XSGe7i0YZQzM2zre5q60iFr7DEI (DNS) Dma1-I194A constitutively localizes to SPB throughout the cell cycle. Dma1-I194A localizes more intensely at one of the two SPBs for most of mitosis ------- COMMENT: f30149c5fcc7f553 7 SJvD0R7KoFjTCbabRjs/mcP/jp0 Figure 2B and C ------- COMMENT: f30149c5fcc7f553 9 +9U0KAAWhidPxfr8K6AizO375Zg Figure S2B ------- COMMENT: f30149c5fcc7f553 11 j7SPHE46pQ0I058bTaC8oJA29bU (comment: Vw, because mutants are related to WT, I changed this to 'normal'), Dma1-I194A-mNeonGreen displays transient loss from SPB during anaphase, just like wildtype Dma1. ------- COMMENT: f30149c5fcc7f553 12 YIaWy3LlAiH4a+Zsdf4iMQAX+zU Full length Dma1 binds to phosphorylated Sid4 peptide. This binding is abolished by Dma1 auto-ubiquitination. ------- COMMENT: f30149c5fcc7f553 13 Cft/vXamKLgcRSL+/uQ477uDPLs (comment: [ dma1 unubiquitinated, sid4 phosphorylated]) When Dma1-GFP is permanently tethered to SPBs by Sid4-GBP-mCherry, cells displayed multi-nucleate and kissing nuclei indicative of SIN and cytokinesis failure. ------- COMMENT: f30149c5fcc7f553 14 n+WqNqZ1rYCnU6yWNDnKNDij2IY Figure S2D dma1-GFP sid4-GBP-mCherry cells are very sick, if not die. ppc89-DUB rescued the synthetic sick phenotype of dma1-GFP sid4-GBP-mCherry. The cells have reduced levels of multi-nucleate and kissing nuclei compared with dma1-GFP sid4-GBP-mCherry. ------- COMMENT: f30149c5fcc7f553 17 PzAZAydsnGnoAybpuv1YVlhmuRo Figure 2D (comment: CHECK in vivo) ------- COMMENT: f30149c5fcc7f553 24 eXWPNIslufPlAy5Ve5KCTnJvMXM Figure S2A ------- COMMENT: f30149c5fcc7f553 25 iP0+3o9S8aIJhn+HFTqxCbMs4DM Figure S2D ------- COMMENT: f30149c5fcc7f553 26 3WTafPUUSD6MZy5BZkGkoqOAZ04 (comment: temporal localization pattern) Figure S2B ------- COMMENT: f30149c5fcc7f553 28 Mc1Dl/8ePjG+W3BQIevC1vaEvqw Figure 2D ------- COMMENT: f30149c5fcc7f553 30 J/3f2o9zLVKsuJumLz4ntYS3kjs (comment: CHECK These data are consistent with auto-ubiquitination triggering Dma1 destruction.) ------- COMMENT: f30149c5fcc7f553 31 1C09U3SGlHvPkvhPIrOlXx1qV8w Figure 2G ------- COMMENT: f30e4e8e80944595 1 LcwRgeLBQMvlrGwq6r+tTxVNQsY The extent of biofilm formed in SPBPJ4664.02∆ decreased by 40% as compared to WT (p < 0.05), indicating that SPBPJ4664.02 is required for biofilm formation. ------- COMMENT: f33df0b48bb1431a 1 lJs+xLHW3PtOQBcpHCpVnrbcCLM (comment: solid media screen using prototroph deletion library.) ------- COMMENT: f33df0b48bb1431a 2 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 3 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 4 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 5 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 6 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 7 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 8 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 9 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 10 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 11 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 12 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 13 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 14 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 15 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 16 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 17 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 18 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 19 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 20 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 21 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 22 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 23 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 24 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 25 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 26 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 27 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 28 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 29 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 30 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 31 lmK6Flnc5rnRPwmIobG5/ABoWjw (comment: solid media screen using prototrophic deletion library) ------- COMMENT: f33df0b48bb1431a 32 dksCFKk1RB4ppmyHxXnto9xRl94 (comment: Data from screening of prototroph deletion library) ------- COMMENT: f33df0b48bb1431a 38 0sRyS9mj78tv98KqAT4rCx5qPfU (comment: Liquid media growth assay. Mutant isolated from deletion collection.) ------- COMMENT: f33df0b48bb1431a 39 0sRyS9mj78tv98KqAT4rCx5qPfU (comment: Liquid media growth assay. Mutant isolated from deletion collection.) ------- COMMENT: f33df0b48bb1431a 40 0sRyS9mj78tv98KqAT4rCx5qPfU (comment: Liquid media growth assay. Mutant isolated from deletion collection.) ------- COMMENT: f33df0b48bb1431a 41 0sRyS9mj78tv98KqAT4rCx5qPfU (comment: Liquid media growth assay. Mutant isolated from deletion collection.) ------- COMMENT: f33df0b48bb1431a 42 0sRyS9mj78tv98KqAT4rCx5qPfU (comment: Liquid media growth assay. Mutant isolated from deletion collection.) ------- COMMENT: f33df0b48bb1431a 44 WQc3Xj/UjccxJJQygPWHaCOZihE (comment: arg3-GFP fusion localisation in minimal media (EMM)) ------- COMMENT: f33df0b48bb1431a 45 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 46 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 47 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 48 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 49 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 50 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 51 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 52 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 53 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 54 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 55 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 56 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 57 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 58 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 59 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 60 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 61 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 62 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 63 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 64 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 65 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 66 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 67 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 68 IHEg1YULWtZHfRdl5OdVZw9yM00 (comment: CHECK EMM media with arginine) ------- COMMENT: f33df0b48bb1431a 69 fYctmYBPdiiH47LeIDuJdo0Fn/s genes detected in screen figure EV2 ------- COMMENT: f33df0b48bb1431a 70 fYctmYBPdiiH47LeIDuJdo0Fn/s genes detected in screen figure EV2 ------- COMMENT: f33df0b48bb1431a 71 fYctmYBPdiiH47LeIDuJdo0Fn/s genes detected in screen figure EV2 ------- COMMENT: f33df0b48bb1431a 72 fYctmYBPdiiH47LeIDuJdo0Fn/s genes detected in screen figure EV2 ------- COMMENT: f33df0b48bb1431a 73 fYctmYBPdiiH47LeIDuJdo0Fn/s genes detected in screen figure EV2 ------- COMMENT: f33df0b48bb1431a 74 fYctmYBPdiiH47LeIDuJdo0Fn/s genes detected in screen figure EV2 ------- COMMENT: f33df0b48bb1431a 75 fYctmYBPdiiH47LeIDuJdo0Fn/s genes detected in screen figure EV2 ------- COMMENT: f33df0b48bb1431a 76 fYctmYBPdiiH47LeIDuJdo0Fn/s genes detected in screen figure EV2 ------- COMMENT: f33df0b48bb1431a 77 fYctmYBPdiiH47LeIDuJdo0Fn/s genes detected in screen figure EV2 ------- COMMENT: f38f39f8014a9104 1 X2FwX52A/VmXoavSQdVzkeQZDsg Whereas the deletion of cox6 critically decreased oxygen consumption in S. pombe, we observed no such effect in S. japonicus (Fig- ure 1A). ------- COMMENT: f38f39f8014a9104 2 JATTpOvZXY6XXTN6EsqXYj2heaM (comment: CHECK Growth rate improved by addition of arginine) ------- COMMENT: f38f39f8014a9104 7 SS2ALO4Gwjqe2OFNMYKWXCuL6rw Strikingly, while the deletion of gpd1 in S. pombe did not negatively affect its growth, regardless of respiratory activity (Figures 1E and S1J), ------- COMMENT: f38f39f8014a9104 8 blBm+B5fQ/eNaR7HOAoOANumU34 Consistent with previous work,63 the higher Pyk1 activity in S. pombe increased the cellular ATP/ADP ratio, whereas the lower Pyk1 activity in S. japonicus reduced it (Fig- ure 3B). ------- COMMENT: f38f39f8014a9104 10 5kP8I8Gxzj85ePN5afwLKcjNRHU (comment: CHECK Growth rate improved by addition of either glutamate, glutamine, or arginine) ------- COMMENT: f38f39f8014a9104 13 H7q94SS7efCIcPT+lnfcaGB3dUM inferred from isotope labelling Figure 1A & We as- sessed the TCA cycle architecture in S. pombe and S. japonicus using stable isotope tracing metabolomics, quantifying the ratios of different TCA-intermediate isotopologs after feeding cells with 13C6-glucose (Figures 2A and 2B). ------- COMMENT: f38f39f8014a9104 14 hYS1sS8c73SpnjhnzK1sFBlEUbA inferred from isotope labelling Figure 1A and We as- sessed the TCA cycle architecture in S. pombe and S. japonicus using stable isotope tracing metabolomics, quantifying the ratios of different TCA-intermediate isotopologs after feeding cells with 13C6-glucose (Figures 2A and 2B). ------- COMMENT: f38f39f8014a9104 15 hYS1sS8c73SpnjhnzK1sFBlEUbA inferred from isotope labelling Figure 1A and We as- sessed the TCA cycle architecture in S. pombe and S. japonicus using stable isotope tracing metabolomics, quantifying the ratios of different TCA-intermediate isotopologs after feeding cells with 13C6-glucose (Figures 2A and 2B). ------- COMMENT: f38f39f8014a9104 16 hYS1sS8c73SpnjhnzK1sFBlEUbA inferred from isotope labelling Figure 1A and We as- sessed the TCA cycle architecture in S. pombe and S. japonicus using stable isotope tracing metabolomics, quantifying the ratios of different TCA-intermediate isotopologs after feeding cells with 13C6-glucose (Figures 2A and 2B). ------- COMMENT: f38f39f8014a9104 17 hYS1sS8c73SpnjhnzK1sFBlEUbA inferred from isotope labelling Figure 1A and We as- sessed the TCA cycle architecture in S. pombe and S. japonicus using stable isotope tracing metabolomics, quantifying the ratios of different TCA-intermediate isotopologs after feeding cells with 13C6-glucose (Figures 2A and 2B). ------- COMMENT: f38f39f8014a9104 18 hYS1sS8c73SpnjhnzK1sFBlEUbA inferred from isotope labelling Figure 1A and We as- sessed the TCA cycle architecture in S. pombe and S. japonicus using stable isotope tracing metabolomics, quantifying the ratios of different TCA-intermediate isotopologs after feeding cells with 13C6-glucose (Figures 2A and 2B). ------- COMMENT: f38f39f8014a9104 19 hYS1sS8c73SpnjhnzK1sFBlEUbA inferred from isotope labelling Figure 1A and We as- sessed the TCA cycle architecture in S. pombe and S. japonicus using stable isotope tracing metabolomics, quantifying the ratios of different TCA-intermediate isotopologs after feeding cells with 13C6-glucose (Figures 2A and 2B). ------- COMMENT: f38f39f8014a9104 20 hYS1sS8c73SpnjhnzK1sFBlEUbA inferred from isotope labelling Figure 1A and We as- sessed the TCA cycle architecture in S. pombe and S. japonicus using stable isotope tracing metabolomics, quantifying the ratios of different TCA-intermediate isotopologs after feeding cells with 13C6-glucose (Figures 2A and 2B). ------- COMMENT: f38f39f8014a9104 21 XHS6pdNvn519SyI6Z3oKvzZDjLk The non-respiring S. pombe cox6D mutant did not upregulate G3P synthesis as compared with the wild-type. S. japonicus also maintained a larger pool of G3P than S. pombe (Figure S1G) ------- COMMENT: f38f39f8014a9104 22 hVIcQ15SISLy1gH312qYM+wForI M + 2 fumarate is typically associated with the oxidative TCA cycle52,57–59 (Figure 2A). Indeed, only respiro-fermenting wild- type S. pombe showed M + 2 fumarate (Figure 2C). M + 3 fuma- rate likely originates from the reductive TCA branch52,57–59 (Fig- ure 2B). ------- COMMENT: f38f39f8014a9104 23 ClfbJoFuFVsIWyhDCzIejgqbAm4 Interestingly, both wild-type and cox6D S. pombe, and wild-type S. japonicus, showed high M + 3 fumarate fractions (Figure 2D). This indicates that not only S. japonicus and non- respiring S. pombe but also the wild-type S. pombe operate the reductive TCA branch. ------- COMMENT: f38f39f8014a9104 24 5kP8I8Gxzj85ePN5afwLKcjNRHU (comment: CHECK Growth rate improved by addition of either glutamate, glutamine, or arginine) ------- COMMENT: f38f39f8014a9104 25 5kP8I8Gxzj85ePN5afwLKcjNRHU (comment: CHECK Growth rate improved by addition of either glutamate, glutamine, or arginine) ------- COMMENT: f38f39f8014a9104 26 5kP8I8Gxzj85ePN5afwLKcjNRHU (comment: CHECK Growth rate improved by addition of either glutamate, glutamine, or arginine) ------- COMMENT: f38f39f8014a9104 27 QKgJcE6ZICBWvT36qy4QYd9i/vk Surprisingly, the growth defect of S. pombe cox6D mutants in EMM could be rescued by arginine but not glutamate or glutamine (Figure 2H). ------- COMMENT: f3a65af2138844d6 14 YGoCbImjetoumOXjnnOtOFrIl7A increased transcriptional response to nitrogen starvation ------- COMMENT: f3a65af2138844d6 16 YGoCbImjetoumOXjnnOtOFrIl7A increased transcriptional response to nitrogen starvation ------- COMMENT: f3a65af2138844d6 41 xBNMIULM14iSAtjYGCMY2UzSKsw (comment: CHECK increased transcription from TR box SO:0001858) ------- COMMENT: f3b2e78baad1483c 1 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: f3b2e78baad1483c 7 aKSuNP01YyCAxQf7IbiB9gen1ZA fig 2b ------- COMMENT: f3b2e78baad1483c 8 aKSuNP01YyCAxQf7IbiB9gen1ZA fig 2b ------- COMMENT: f3c7bd963b3b7da5 13 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: f3c7bd963b3b7da5 14 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: f3c7bd963b3b7da5 15 TUQwAY89q7R65MtiM+SwHcYaCsA fig4D ------- COMMENT: f3c7bd963b3b7da5 19 gXMLfLAgqvyuQK6t7G0cT5WGON4 fig 7: "unlike the hem13-1 mutant, the hem12 and hem14 null mutants of the heme biosynthesis pathway are insensitive to HU" ------- COMMENT: f3c7bd963b3b7da5 20 gXMLfLAgqvyuQK6t7G0cT5WGON4 fig 7: "unlike the hem13-1 mutant, the hem12 and hem14 null mutants of the heme biosynthesis pathway are insensitive to HU" ------- COMMENT: f3db412b3e0f395f 1 E3HXkGKmAvMiN8QFdhDGwf50Fnc figure 1b ------- COMMENT: f3db412b3e0f395f 2 0UGhnGrUG/kTzdjdxv9NINeD+zs figure1a ------- COMMENT: f3db412b3e0f395f 3 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: f3db412b3e0f395f 4 MVq1hSbUxiQHwPRh+dsw/ul0Uuw figure 1a ------- COMMENT: f3db412b3e0f395f 5 0UGhnGrUG/kTzdjdxv9NINeD+zs figure1a ------- COMMENT: f3db412b3e0f395f 6 0UGhnGrUG/kTzdjdxv9NINeD+zs figure1a ------- COMMENT: f3db412b3e0f395f 7 0UGhnGrUG/kTzdjdxv9NINeD+zs figure1a ------- COMMENT: f3db412b3e0f395f 8 5UBwtFapOe87NCyLHI/PsFUarqo figure 1c Interestingly, no additive effect was seen in the bub11–179 sgo2D double mutant, indicating that Sgo2 and the kinase domain of Bub1 act in the same pathway (Figure 3A). ------- COMMENT: f3db412b3e0f395f 9 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: f3db412b3e0f395f 10 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: f3db412b3e0f395f 11 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: f3db412b3e0f395f 12 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: f3db412b3e0f395f 13 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: f3db412b3e0f395f 14 lRBB1u0l/jOf62C8SFj3SuRjJEk , Sgo1 is seen as nuclear staining with punctate dots of fluores- cence along the spindle (Figure 1D and [2, 9]). This local- ization is abolished in a bub1D background but pre- served in the truncated mutants (Figure 1D), although the signal intensity was variable; in bub11–585, Sgo1 staining was as strong as in the wild-type, but it was weaker in bub11–179 and bub11–826 backgrounds. We conclude that the N terminus of Bub1 is sufficient to pro- mote correct Sgo1 localization and function during MI. ------- COMMENT: f3db412b3e0f395f 15 lRBB1u0l/jOf62C8SFj3SuRjJEk , Sgo1 is seen as nuclear staining with punctate dots of fluores- cence along the spindle (Figure 1D and [2, 9]). This local- ization is abolished in a bub1D background but pre- served in the truncated mutants (Figure 1D), although the signal intensity was variable; in bub11–585, Sgo1 staining was as strong as in the wild-type, but it was weaker in bub11–179 and bub11–826 backgrounds. We conclude that the N terminus of Bub1 is sufficient to pro- mote correct Sgo1 localization and function during MI. ------- COMMENT: f3db412b3e0f395f 16 lRBB1u0l/jOf62C8SFj3SuRjJEk , Sgo1 is seen as nuclear staining with punctate dots of fluores- cence along the spindle (Figure 1D and [2, 9]). This local- ization is abolished in a bub1D background but pre- served in the truncated mutants (Figure 1D), although the signal intensity was variable; in bub11–585, Sgo1 staining was as strong as in the wild-type, but it was weaker in bub11–179 and bub11–826 backgrounds. We conclude that the N terminus of Bub1 is sufficient to pro- mote correct Sgo1 localization and function during MI. ------- COMMENT: f3db412b3e0f395f 17 lRBB1u0l/jOf62C8SFj3SuRjJEk , Sgo1 is seen as nuclear staining with punctate dots of fluores- cence along the spindle (Figure 1D and [2, 9]). This local- ization is abolished in a bub1D background but pre- served in the truncated mutants (Figure 1D), although the signal intensity was variable; in bub11–585, Sgo1 staining was as strong as in the wild-type, but it was weaker in bub11–179 and bub11–826 backgrounds. We conclude that the N terminus of Bub1 is sufficient to pro- mote correct Sgo1 localization and function during MI. ------- COMMENT: f3db412b3e0f395f 18 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: f3db412b3e0f395f 19 wD6/8UKsN58lqrqDOeNcG32tbVc figure1c a high frequency of sis- ter-chromatid nondisjunction during MII (Figure 1C), consistent with Sgo1’s being largely nonfunctional in this mutant background. ------- COMMENT: f3db412b3e0f395f 20 WNze3qhtq0a98hpPomFUr8gAIps Similarly, we found that Sgo1 was mislocalized in a different allele (Figure 1D, K762M [6]) ------- COMMENT: f3db412b3e0f395f 21 MJs4qeQNz68xEJREzGtHlbR8U8g However, we found that Bub1K762M was properly localized in metaphase I cells and that the amount of protein at centromeres was close to wild-type levels (Figure S1). ------- COMMENT: f3db412b3e0f395f 22 CB5aDggBc/H4Ggu1u/ClNf24eGU figure 3a Interestingly, no additive effect was seen in the bub11–179 sgo2D double mutant, indicating that Sgo2 and the kinase domain of Bub1 act in the same pathway (Figure 3A). ------- COMMENT: f3db412b3e0f395f 23 FsRsBMG6d4/HYwyLFkfBR4X+aWY Interestingly, no additive effect was seen in the bub11–179 sgo2D double mutant, indicating that Sgo2 and the kinase domain of Bub1 act in the same pathway (Figure 3A). ------- COMMENT: f3db412b3e0f395f 24 HrcASN13y6oqFTlq7TszCzx05Ts As reported previously [13], expression of Rec8RDRD in wild-type cells prevented homolog segregation (no bi- nucleate cells), but homolog segregation was restored to a certain extent by the deletion of rec11 (approxi- mately 40% binucleated cells). ------- COMMENT: f3db412b3e0f395f 25 jqv7NXfhQjrSjJPzBCaZ6j0QTes but homolog segregation was restored to a certain extent by the deletion of rec11 (approxi- mately 40% binucleated cells). ------- COMMENT: f3e92e0c38be784b 1 01cZnK1Zl/UgBOPhk8pEpkrqXr0 (comment: CONDITION Ricinoleic acid, RA moieties from phospholipids) ------- COMMENT: f43a3b416d8fede7 2 jDkf3BY8QZoDiQFYRfOpz+loTcA mto2 deletion strain, which yielded viable but slightly bent cells (Fig. 3 A) ------- COMMENT: f43a3b416d8fede7 3 jDkf3BY8QZoDiQFYRfOpz+loTcA mto2 deletion strain, which yielded viable but slightly bent cells (Fig. 3 A) ------- COMMENT: f43a3b416d8fede7 4 StQxl24jcjygfv3HR0az1oAsW4E The average number of MT bundles in mto2Δ cells (n = 1.3 ± 0.7 SD; Fig. 3 E) was significantly lower than in wild-type cells (3.6 ± 0.9) ------- COMMENT: f4425b94136060a0 5 1d+JlPI6jHRaw3fFpqVR3umFcVQ (comment: assayed substrate casein) ------- COMMENT: f4425b94136060a0 6 39uInX2GSzMMKO1On2LDbUiJ8qQ (comment: assayed substrate MBP) ------- COMMENT: f4425b94136060a0 7 39uInX2GSzMMKO1On2LDbUiJ8qQ (comment: assayed substrate MBP) ------- COMMENT: f4425b94136060a0 8 39uInX2GSzMMKO1On2LDbUiJ8qQ (comment: assayed substrate MBP) ------- COMMENT: f4425b94136060a0 17 qQ419y/H+VtLoFoZW5fJh9ywaeo Fig. 1A and Table I) ------- COMMENT: f4425b94136060a0 20 39uInX2GSzMMKO1On2LDbUiJ8qQ (comment: assayed substrate MBP) ------- COMMENT: f4425b94136060a0 21 39uInX2GSzMMKO1On2LDbUiJ8qQ (comment: assayed substrate MBP) ------- COMMENT: f4425b94136060a0 22 39uInX2GSzMMKO1On2LDbUiJ8qQ (comment: assayed substrate MBP) ------- COMMENT: f4425b94136060a0 23 39uInX2GSzMMKO1On2LDbUiJ8qQ (comment: assayed substrate MBP) ------- COMMENT: f4425b94136060a0 84 B2Ukc4z6tgggzk2jFUHQ8GxzsvY (comment: CHECK negative reg of polarization/remodelling) ------- COMMENT: f44eccf92770b920 1 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: f44eccf92770b920 2 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: f44eccf92770b920 3 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: f44eccf92770b920 4 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: f44eccf92770b920 5 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: f44eccf92770b920 6 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: f44eccf92770b920 7 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: f44eccf92770b920 8 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: f44eccf92770b920 9 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: f44eccf92770b920 10 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: f44eccf92770b920 11 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: f44eccf92770b920 12 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: f44eccf92770b920 13 2uio3TuL4fC3ahN6fUzHuREDGPc Fig. S2 ------- COMMENT: f44eccf92770b920 14 hPX9iy7PYMSiUr07SyBmn/UVtW8 (comment: ChIP-seq); Fig. 1C, 1D ------- COMMENT: f44eccf92770b920 15 XkM6BIgN7eQfW4X4n5W7UXFrqEI Fig. 2A, 2B ------- COMMENT: f44eccf92770b920 16 yOh8cxX2brAi1iM3aDT9gU7I/G8 Fig. 2E, 2F ------- COMMENT: f44eccf92770b920 17 yOh8cxX2brAi1iM3aDT9gU7I/G8 Fig. 2E, 2F ------- COMMENT: f44eccf92770b920 18 yOh8cxX2brAi1iM3aDT9gU7I/G8 Fig. 2E, 2F ------- COMMENT: f45b7c9c20201a38 207 0tBPgayIHCdo4HdNFNQyrD9meWQ Figure 2, 4 These results indicated that the Gih35 helicase is part of the Gih35 and Wdr83 on one side, and as an anchoring protein that allows the binding of the Gpl1-Gih35-Wdr83 complex to the spliceosome on the other side. Gpl1- Gih35-Wdr83 complex, but to associate with the spliceosome, it requires the interaction with Gpl1. Altogether, these findings confirmed the above results of the Y2H assay and provided further support for the hypothesis that Gpl1 functions as a bridging protein for ------- COMMENT: f45b7c9c20201a38 208 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: f45b7c9c20201a38 209 1DYV2VCxnLwB/LbJ8mYtGzsWbZ8 (comment: Gpl1-Gih35-Wdr83 complex) ------- COMMENT: f45b7c9c20201a38 210 1DYV2VCxnLwB/LbJ8mYtGzsWbZ8 (comment: Gpl1-Gih35-Wdr83 complex) ------- COMMENT: f45b7c9c20201a38 211 1DYV2VCxnLwB/LbJ8mYtGzsWbZ8 (comment: Gpl1-Gih35-Wdr83 complex) ------- COMMENT: f45b7c9c20201a38 212 YnI3sHg7ttDSlmqEiY2AgChnjTc figure3 ------- COMMENT: f45b7c9c20201a38 213 YnI3sHg7ttDSlmqEiY2AgChnjTc figure3 ------- COMMENT: f45b7c9c20201a38 214 YnI3sHg7ttDSlmqEiY2AgChnjTc figure3 ------- COMMENT: f45b7c9c20201a38 215 YnI3sHg7ttDSlmqEiY2AgChnjTc figure3 ------- COMMENT: f45b7c9c20201a38 216 D4WuxjqCKpmJjpV+fd5XoyXAec0 We detected wdr83 (Figure S1) and performed the Western blot and RT-qPCR analyses. We detected no significant changes in the mRNA levels of gpl1, gih35 or wdr83 in the analyzed mu- no significant changes in the mRNA levels of gpl1, gih35 or wdr83 in the analyzed mutants tants (Figure 5a). ------- COMMENT: f46092d2d0683328 1 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: f46092d2d0683328 2 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: f46092d2d0683328 3 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: f46092d2d0683328 5 GSDoKVHrCsiUO6zYP0Vg8a8VAAQ fig 1D ------- COMMENT: f46092d2d0683328 6 QxyA7Kxt4u0SZftovXPYBddoxhU Fig 1D ------- COMMENT: f46092d2d0683328 7 QxyA7Kxt4u0SZftovXPYBddoxhU Fig 1D ------- COMMENT: f46092d2d0683328 8 EvCK9LcQYelMCHeU/9caH0xlskg Fig 1D (comment: pom1 is catalytically active but not localized to cell ends) ------- COMMENT: f46092d2d0683328 9 +BfEGlhGr32B69INxqwJVJVrajE Fig 1 ------- COMMENT: f46092d2d0683328 10 KV3JZVyKyknYPoBVnGTrcnQeKhc (comment: old end) ------- COMMENT: f46092d2d0683328 12 7aiEgOiz1UiezZrFgpNqBk1miYs Fig1SE to cell cortex of (newnon growing) cell tip from medial cortex ------- COMMENT: f46092d2d0683328 13 pZrCCBUH2wjW4S4NhoXLRB4xIPo FigS1D ------- COMMENT: f46092d2d0683328 14 YOeUEwdTmh6sb1FTcxk0N0it7gU Fig1SE to cell cortex of (new) cell tip from medial cortex ------- COMMENT: f46092d2d0683328 15 YOeUEwdTmh6sb1FTcxk0N0it7gU Fig1SE to cell cortex of (new) cell tip from medial cortex ------- COMMENT: f46092d2d0683328 16 N5iyUwdxEZ4wQQ1PrkqW4eij1Cs Fig 2C ------- COMMENT: f46092d2d0683328 17 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: f46092d2d0683328 18 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: f46092d2d0683328 20 L4VwRgjWnfQo6lfFahjfe3XN9NE (comment: CHECK GTP bound) ------- COMMENT: f46092d2d0683328 21 ZndFtOaWWWzi3RkVisV3jIo6DoA (comment: CHECK GTP bound active form) ------- COMMENT: f46092d2d0683328 22 /gNxO7aU1IFyCg5pvY74dYgywd4 (comment: CHECK active GTP bound form) ------- COMMENT: f46092d2d0683328 23 NIlypNDW5im+EGYoY3VVzYpSMXA (comment: CHECK GTP bound) fig 3C ------- COMMENT: f46092d2d0683328 24 wf185u4DRDkg7Sbpsn3gLe6+2NY Figure 4E ------- COMMENT: f46092d2d0683328 25 TOCv67VmypboNgTj2uF490e6wJ4 (comment: CHECK GTP-bound) Figure 4E ------- COMMENT: f46092d2d0683328 26 ww7Yg+6JSSSkec/v9ebBjHji5v8 (comment: CHECK GTP-bound) Figure 4E, polarization localization to both cell ends ------- COMMENT: f46092d2d0683328 30 vgtY207xllJXDikZBpU+h3x+zS0 (comment: *****OLD*****waiting for GO) ------- COMMENT: f48d48d052e6ec31 4 nfAfzGlpHM9PGFVFbHEvS2mJPtY (comment: was branched, elongated, multiseptate cell ) ------- COMMENT: f4d5b599093ab44e 1 7TvRD+Kq2oNaIDb3jvzank0j+Qk fig 1a (comment: CHECK thinner discontinuous spindles fypo/issues/3208) ------- COMMENT: f4d5b599093ab44e 3 b1eTMKRJxcIdQ5TVEYl3e/QXO84 Fig. 1c, cells 1 and 3 Furthermore, multiple Mad2 dots, which have never been seen in wild type cells ------- COMMENT: f4d5b599093ab44e 4 wKE5RGUg8tMlZ5dNLHodHLKYsb4 Fig 1d ------- COMMENT: f4d5b599093ab44e 5 Ph/zIJtd4Zv8qSekboJt3wsN8JU Fig 1e ------- COMMENT: f4d5b599093ab44e 9 wKE5RGUg8tMlZ5dNLHodHLKYsb4 Fig 1d ------- COMMENT: f4d5b599093ab44e 10 wKE5RGUg8tMlZ5dNLHodHLKYsb4 Fig 1d ------- COMMENT: f4d5b599093ab44e 11 wKE5RGUg8tMlZ5dNLHodHLKYsb4 Fig 1d ------- COMMENT: f4da3c52016e8dca 1 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: f4da3c52016e8dca 10 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: f4da3c52016e8dca 11 43nEOIFlZ9esynf/SOHRc4AY4CA fig 1A, 1B ------- COMMENT: f4da3c52016e8dca 15 lVvMgkFNoEp4aURnzoIpcy7J//0 fig 5A ------- COMMENT: f4da3c52016e8dca 17 XDYaRVbi2ve77LI3iO6QXyucMgk fig S2C ------- COMMENT: f4da3c52016e8dca 18 FkncP3MM9Jybc3z7XaxJB+ycFPE Fig 1B ------- COMMENT: f4da3c52016e8dca 19 VC9cotctNaroCl7uUTqeL/En5oc Figure 1B ------- COMMENT: f4da3c52016e8dca 20 o1GdUlMekysn+Q2mPEPtBAfEJKI (comment: CHECK i requested reduced plasma membrane PIP issues/3117 GFP-2xPH(Plc delta)) "localization The PI(4,5)P2 sensor GFP-2×PH(PLCδ) (Stefan et al., 2002) was reduced at the cell cortex and the division site in efr3Δ compared with WT (Fig. 1 D), indicating that PIP PM abundance is reduced in efr3Δ" ------- COMMENT: f4da3c52016e8dca 21 qRuiFIUfCEaRK2U9+oSKa18znxI Figure 3E ------- COMMENT: f4da3c52016e8dca 22 LQmuKZbL9ND56pzzWctDT58tWRE Figure 3C ------- COMMENT: f4da3c52016e8dca 23 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: f4da3c52016e8dca 24 8zdno63XFs3BFsVP161LRtfCMNM Figure 3D ------- COMMENT: f4da3c52016e8dca 25 CPtfNCCJL1ySb3M6MxHXDn+E2Wc Figure S1D ------- COMMENT: f4da3c52016e8dca 26 gC+OPtCC43GyDCtaG9nh6Kn4Rmc Figure S1C ------- COMMENT: f4da3c52016e8dca 27 CUuhvl0QV28Id9dVbEOUmTYYRaE Figure S2A, S2B ------- COMMENT: f4da3c52016e8dca 28 CUuhvl0QV28Id9dVbEOUmTYYRaE Figure S2A, S2B ------- COMMENT: f4da3c52016e8dca 29 rzoapsxxo5AQc2NAakt5rfXh8WQ Figure S2E ------- COMMENT: f4da3c52016e8dca 30 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: f4da3c52016e8dca 31 qS141CgI6QwEv91oajAkM7+sA9M Figure 1C ------- COMMENT: f4da3c52016e8dca 32 0MXTQLBqD6xBsayLK0zY5b1fbqs Fig. S1C, S1D) ------- COMMENT: f4da3c52016e8dca 33 0MXTQLBqD6xBsayLK0zY5b1fbqs Fig. S1C, S1D) ------- COMMENT: f4da3c52016e8dca 34 qThf9cfwXYp6YeRBqMR23j/d8cU Fig 2A-C ------- COMMENT: f4da3c52016e8dca 35 qThf9cfwXYp6YeRBqMR23j/d8cU Fig 2A-C ------- COMMENT: f4da3c52016e8dca 38 qThf9cfwXYp6YeRBqMR23j/d8cU Fig 2A-C ------- COMMENT: f4da3c52016e8dca 39 yKshcWa36JVyzon7b5A8sSAsc+Y Fig. S2D ------- COMMENT: f4da3c52016e8dca 43 G7u8tVQQ2C+cl9b+ZF4Xx3nBHhc Fig 3A (in table, data not shown) ------- COMMENT: f4da3c52016e8dca 46 f4NHKMAl7Go72p+0Yku+mCWKkz8 fig 5B CR slid- ing events no longer occurred in myo51Δ efr3Δ ------- COMMENT: f4da3c52016e8dca 47 f4NHKMAl7Go72p+0Yku+mCWKkz8 fig 5B CR slid- ing events no longer occurred in myo51Δ efr3Δ ------- COMMENT: f4da3c52016e8dca 50 rm9DpUUq199lzNzYKV18pe0sBF8 (comment: phospholipid biosynthesis?) ------- COMMENT: f4da3c52016e8dca 52 XVmNRxkD76KTg6hmsNuqoXuzdZg (comment: naintenence) ------- COMMENT: f507eb8aa144ccde 3 eHzm5a+Ik9xG3SJaIBiohDMopuU Fig. 2c and Table 1), ------- COMMENT: f507eb8aa144ccde 5 OaIxT4A2lKxv9K7F+2F1/+D6PV0 The most straightforward interpretation is that Bub1 is required to maintain sister-chromatid cohesion at anaphase I by preventing the removal of Rec8 from centromeric regions. ------- COMMENT: f507eb8aa144ccde 13 gm1Px94b5UBdjxqoHSq63NZdxOQ table1 ------- COMMENT: f507eb8aa144ccde 14 u+jdHbGb9/snsm8MZMgL7msTTQg table1 Interestingly, sister chromatids do not segregate randomly in the absence of Rec8 but rather segregate equationally10 (Table 1), implying that cohesion must be preserved between sister centromeres to give them a mitotic-like, back-to-back orientation ------- COMMENT: f507eb8aa144ccde 15 fDVeg09sF+JKl3UgkT5LeANWsrI table1 Together, these results rule out the possibility that equational segregation in the absence of Bub1 is due to loss of sister-chromatid cohesion before attachment of kinetochores to microtubules. ------- COMMENT: f507eb8aa144ccde 16 3lJXiXJOrH4lN0QHyN8Ig3vxxpQ Consistently, Rec8 localization was indistinguishable from wildtype from early meiosis and until metaphase I (Fig. 3). Rec8 first appeared in the centromeric regions of cells before conjugation (Fig. 3, G1 cells) and its distribution was further extended throughout chromatin during the horse-tail stage and until metaphase I. Therefore, Rec8 is properly localized and co-orientation still occurs in the absence of Bub1, indicating that the occurrence of equational segregation in Dbub1 cells may be due to a defect in functional fusion of sister kinetochores rather than to defective co-orientation of sister centromeres. ------- COMMENT: f507eb8aa144ccde 17 lCVkjKc/LFnJZOs8u+l8t1sRKBw In contrast, Rec8 disappeared completely in Dbub1 anaphase I cells. Rec8 was not observed in any of the >100 late-anaphase cells examined and was obviously never detected in early MII cells ------- COMMENT: f507eb8aa144ccde 18 lCVkjKc/LFnJZOs8u+l8t1sRKBw In contrast, Rec8 disappeared completely in Dbub1 anaphase I cells. Rec8 was not observed in any of the >100 late-anaphase cells examined and was obviously never detected in early MII cells ------- COMMENT: f5403fffd3ecaf81 15 oKLxbzKo0/WVGWIDanEz3pGJovg (comment: CHECK NEW TERM REQUESTED CHILD OF BOTH termination of RNA polymerase II transcription, poly(A)-independent) We showed pac1-dependent poly(A)-independent RNA polymerase II termination for 2 mRNA genes (mfs2 and SPBC530.02), 4 snRNA genes (snU1, snU2, snU4 and snU5), and 2 snoRNA genes (snU3 and snU32) . (comment: Can this be added in the annotation extension ?) ------- COMMENT: f5403fffd3ecaf81 20 icd1dUAAJDwHGUfRx+7mJ0sNpNM (comment: CHECK waiting for GO:NEW.) We show this for only two mRNA: mfs2 and SPBC530.02. (comment: Can this be added in the annotation extension ?) ------- COMMENT: f5403fffd3ecaf81 21 g+FxslB3+0eM4UbGdyIVeHs7ims Supplementary Figure S5B ------- COMMENT: f5403fffd3ecaf81 25 8KnndykYj3bctZ6PUi+9uLr3K04 Figure 3A and Supplementary Fig- ure S5A ------- COMMENT: f5403fffd3ecaf81 26 8KnndykYj3bctZ6PUi+9uLr3K04 Figure 3A and Supplementary Fig- ure S5A ------- COMMENT: f5403fffd3ecaf81 29 ViyrEX8oQ/W8/L3ac6o3yHEwvuY figure 1c ------- COMMENT: f5403fffd3ecaf81 30 o+f+DiccYhz7u8xMlwAl0Oil5h0 (comment: 5' extended precursors, C/C box (but not H/ACA box).) Pac1 nuclear exclusion specifically led to the accumulation of 5′-extended precursors of Pac1-bound C/D box snoRNAs (Supplementary Figure S3A). This ac- cumulation was confirmed by Northern blot assays on three C/D box snoRNAs (sno16, snoU14 and snr79), whereas a control H/ACA box snoRNA (sno12) was unaffected ------- COMMENT: f5403fffd3ecaf81 31 tahTaZzwZTCUtpyLlAPo20OYJWA (comment: 5' extended precursors, C/C box (but not H/ACA box)) ------- COMMENT: f5403fffd3ecaf81 32 tahTaZzwZTCUtpyLlAPo20OYJWA (comment: 5' extended precursors, C/C box (but not H/ACA box)) ------- COMMENT: f5403fffd3ecaf81 33 qXJxuqk9d01wBW5DbxIx/rYdYEs we observed a sharp decline in RNAPII occupancy inside the gene body, directly downstream of the Pac1- bound region located in the first half of the genes (Fig- ure 2B, blue profile). In contrast, Pac1 nuclear exclusion resulted in extended RNAPII occupancy throughout the entire ORFs (Figure 2B, red profile). Such differences in RNAPII profiles are suggestive of Pac1-dependent prema- ture termination. ------- COMMENT: f5403fffd3ecaf81 35 I0nQOM+ry54A3AebIbNXG+cMpNg (comment: CHECK waiting for GO:NEW.)inferred from association with prremature termination sites ------- COMMENT: f5403fffd3ecaf81 37 qXJxuqk9d01wBW5DbxIx/rYdYEs we observed a sharp decline in RNAPII occupancy inside the gene body, directly downstream of the Pac1- bound region located in the first half of the genes (Fig- ure 2B, blue profile). In contrast, Pac1 nuclear exclusion resulted in extended RNAPII occupancy throughout the entire ORFs (Figure 2B, red profile). Such differences in RNAPII profiles are suggestive of Pac1-dependent prema- ture termination. ------- COMMENT: f5403fffd3ecaf81 38 6JxPhs1uH5CA5LAMMf79941bHQo (comment: MOVE DOWN) ------- COMMENT: f5403fffd3ecaf81 39 6JxPhs1uH5CA5LAMMf79941bHQo (comment: MOVE DOWN) ------- COMMENT: f5403fffd3ecaf81 40 UZgbhgCQh63nLRanw/R/0dGOzxY Pac1 strain (Pac1-AA) that allowed rapid rapamycin-dependent nuclear exclusion of Pac1 (Figure 1B). ------- COMMENT: f54275f3a8758cf5 3 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: f54275f3a8758cf5 5 aHH98Y5slOMW7n/EjJb3XN+mOLs Figure 2 and 3 ------- COMMENT: f54275f3a8758cf5 6 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: f54275f3a8758cf5 7 aHH98Y5slOMW7n/EjJb3XN+mOLs Figure 2 and 3 ------- COMMENT: f54275f3a8758cf5 11 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: f54275f3a8758cf5 13 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: f54275f3a8758cf5 14 9QAPaSSLpSrZtj4kLjZereKyBGg Figure 2, (comment: detected by northern blot analysis) ------- COMMENT: f54275f3a8758cf5 15 HTBQHo94DfUeTArS9tyUuxU9AfU Figure 2 and S3 ------- COMMENT: f54275f3a8758cf5 22 Ocx+iB/95EBAbk5q7f3QrSYRqPw Figure 1c ------- COMMENT: f54275f3a8758cf5 23 HTBQHo94DfUeTArS9tyUuxU9AfU Figure 2 and S3 ------- COMMENT: f54275f3a8758cf5 24 HTBQHo94DfUeTArS9tyUuxU9AfU Figure 2 and S3 ------- COMMENT: f54393989e565d1a 6 9JK5yJsKAvy9BEyUGpnmaBnEq4c (comment: assayed at ars1 and ars2004, early-firing origins) ------- COMMENT: f54393989e565d1a 7 RCC1sfnm8C7rpbtcs72Q4GkB9sw (comment: assayed at ars1 and ori1-200, early-firing origins; only affects origins normally bound by Mrc1) ------- COMMENT: f54393989e565d1a 11 MMOgavRIJECoOL2ktEXmrigAWaQ (comment: assayed elongation from ars1 and ars2004, early-firing origins) ------- COMMENT: f54393989e565d1a 12 9JK5yJsKAvy9BEyUGpnmaBnEq4c (comment: assayed at ars1 and ars2004, early-firing origins) ------- COMMENT: f54393989e565d1a 13 Kb+cyHEotNkfMjWo3QZV+agJjyw (comment: predominantly at early-firing origins including ars1 and ars2004, but not AT1041; Mrc1 associates with origins later than MCM complex, but slightly earlier than Cdc45) ------- COMMENT: f54393989e565d1a 15 k2y1oYca2/KzHCQxy3capEBeB3g (comment: assayed elongation from ori1-200) ------- COMMENT: f54393989e565d1a 16 PE54oxCFTDaIs0yHXk4O83PnfP4 (comment: assayed at ars1 and ars2004) ------- COMMENT: f54393989e565d1a 17 PE54oxCFTDaIs0yHXk4O83PnfP4 (comment: assayed at ars1 and ars2004) ------- COMMENT: f54393989e565d1a 18 PE54oxCFTDaIs0yHXk4O83PnfP4 (comment: assayed at ars1 and ars2004) ------- COMMENT: f55fcc98c5e448a0 1 ENXWLada3c/w37zT0Mc42XDb0ss (comment: CHECK activated_by(CHEBI:18420)) ------- COMMENT: f592b2691e524e16 1 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: f592b2691e524e16 2 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: f592b2691e524e16 3 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: f592b2691e524e16 4 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: f5cf2ad5297d65c9 1 1SfNVkP7+OjuGvX5brsWm6tLhJ8 (comment: CHECK ksg1-208) ------- COMMENT: f5cf2ad5297d65c9 2 ROOd+m3GAS5CHo3GQ4kAMYHukIY fig2 (comment: septation index, ksg1-208) ------- COMMENT: f5cf2ad5297d65c9 3 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: f5cf2ad5297d65c9 4 z210TF0ewzOVHymKEUkQagjGZuw (Figure 2A) significantly longer than that of wild-type cells at 27◦C ------- COMMENT: f5cf2ad5297d65c9 5 Ro0BF5UBEQ+mwHHq+04EpxYDajo (Figure 2B) ...which was recovered by the overexpression of ppk21+ ------- COMMENT: f5cf2ad5297d65c9 6 LxH0zx9TKEezNO8ukZWz91gBvXI Figure 2B...which was recovered by the overexpression of ppk21+ ------- COMMENT: f5cf2ad5297d65c9 7 4ccY7uJHzPmXasBDFbEIVbzWMpU (Figure 2C) (comment: CONDITION 33 degrees) ------- COMMENT: f5cf2ad5297d65c9 9 HmD4TCtVuomJpKqZJ5xoBwRkPwU (Figures 2D, 2E) results showed that ksg1-208 􏰀ppk21 cells exhibited a longer cell length than either ksg1-208 or 􏰀ppk21 cells at both 27 and 33◦C ------- COMMENT: f5cf2ad5297d65c9 10 YqCWLYpuTullB4N83hB1jTJxnI4 (Figure 2D and Supplementary Figure 1) The results showed that the septation ring of the ksg1-208 delta-ppk21 double mutant was off-centered at 33◦C, which was more severe than that of ksg1-208 cells ------- COMMENT: f5cf2ad5297d65c9 11 q2ZCDNLMvRvLSTvgfeg70Tb8lYI (Figures 3A, 3B) overexpression of cdr2+ also reversed the defects in the cell length and the septation index of ksg1-208 cells ------- COMMENT: f5cf2ad5297d65c9 12 6nmLY/VP8e3okQnqkHVvMu1sQcQ (Figure 3C). In addition, the cdr2+ overexpressed wild-type cells showed a higher septation index than cdr2+ non-overexpressed wild-type cells at 35◦C ------- COMMENT: f5cf2ad5297d65c9 13 Fkso0bHqrEje5FBeswKlfzqN2AA It should be noted that cdr2+ overexpressed wild-type cells showed a shorter cell length at 27◦C, but a longer cell length at 35◦C than cdr2+ non-overexpressed wild-type cells (Figure 3B). ------- COMMENT: f5cf2ad5297d65c9 14 rAtB83vIwXBBPYhRmQb4Sx6xAOk (Figures 4A, 4B) we found that the protein level of Cdr2 in ksg1-208 cells was significantly lower than that in wild-type cells ------- COMMENT: f5cf2ad5297d65c9 15 AgN1w3NJhM2SbZNMIQ1neBlucWY (Figure 5A). we found that the protein level of Cdr2 in ksg1-208 cells was significantly lower than that in wild-type cells (Figures 4A, 4B), ------- COMMENT: f5cf2ad5297d65c9 16 ayUn5lJKxqYosdcbEBWBaGT9Ccs Figure 4c In contrast, a fraction of ksg1-208 cells showed septum or division site localized Cdr2-mEGFP in the dividing cells at 27◦C, indicating the cortex dissociation of Cdr2 was hindered. ------- COMMENT: f5cf2ad5297d65c9 18 Vj+Tl7/FUSKgM9PFZY3hadyX0Mk (Figure 5A) Western blot analysis showed that the level of Cdc25 protein was dramatically lower in ksg1-208 cells than that in wild-type cells, indicating that Ksg1 played a crucial role in the accumulation of Cdc25 protein ------- COMMENT: f5cf2ad5297d65c9 19 WNiswpNllDvJ8mF47IRx1UTEz/Q (Figure 5A) In addition, the Cdc25 protein level decreased in 􏰀ppk21 and 􏰀cdr2 cells as well, indicating the role of Ppk21 and Cdr2 on regulating Cdc25 protein level . ------- COMMENT: f5cf2ad5297d65c9 20 gA2WA1YhiIrn27ebhQtUHcqQBno (Figure 5A). In addition, the Cdc25 protein level decreased in 􏰀ppk21 and 􏰀cdr2 cells as well, indicating the role of Ppk21 and Cdr2 on regulating Cdc25 protein level ------- COMMENT: f5cf2ad5297d65c9 21 D1XrYprRIzKP6TRRM4m6nvHJC4k Figure 1A ------- COMMENT: f5cfe860f60d0109 1 Rk3HxlIx6MRRMiRW0gsUkI2HB+4 Fig. 5G ------- COMMENT: f5cfe860f60d0109 2 T44vR21/o+u/qtfx00leUPdjBlI Fig. 5G ------- COMMENT: f5cfe860f60d0109 3 xrnc8yKTfw/T29zPEjGa7ZRaBeM Fig. 7D ------- COMMENT: f5cfe860f60d0109 4 yKshcWa36JVyzon7b5A8sSAsc+Y Fig. S2D ------- COMMENT: f5cfe860f60d0109 5 yKshcWa36JVyzon7b5A8sSAsc+Y Fig. S2D ------- COMMENT: f5cfe860f60d0109 6 Pn3srtjJ+VcfV3crGgCziQjU7+c Fig. 1D ------- COMMENT: f5cfe860f60d0109 7 7Ub1+CI9H9KZmMOQfFysIkJm17c Fig. 1C ------- COMMENT: f5cfe860f60d0109 8 7Ub1+CI9H9KZmMOQfFysIkJm17c Fig. 1C ------- COMMENT: f5cfe860f60d0109 10 zAapkmbL7f1tFqFVVuI7L+PD6cc Fig. 1E ------- COMMENT: f5cfe860f60d0109 11 zAapkmbL7f1tFqFVVuI7L+PD6cc Fig. 1E ------- COMMENT: f5cfe860f60d0109 12 Pn3srtjJ+VcfV3crGgCziQjU7+c Fig. 1D ------- COMMENT: f5cfe860f60d0109 13 zAapkmbL7f1tFqFVVuI7L+PD6cc Fig. 1E ------- COMMENT: f5cfe860f60d0109 14 7Ub1+CI9H9KZmMOQfFysIkJm17c Fig. 1C ------- COMMENT: f5cfe860f60d0109 15 WeK7yxLE1WYcbMMFfcCh4W0c0Ns Fig. 2D ------- COMMENT: f5cfe860f60d0109 16 WeK7yxLE1WYcbMMFfcCh4W0c0Ns Fig. 2D ------- COMMENT: f5cfe860f60d0109 17 WeK7yxLE1WYcbMMFfcCh4W0c0Ns Fig. 2D ------- COMMENT: f5cfe860f60d0109 18 WeK7yxLE1WYcbMMFfcCh4W0c0Ns Fig. 2D ------- COMMENT: f5cfe860f60d0109 19 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 20 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 21 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 22 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 23 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 24 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 25 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 26 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 27 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 28 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 29 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 30 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 31 hIqFTUnTYawUIyfapMfLIZ5LU1c Fig. 5A, 5C ------- COMMENT: f5cfe860f60d0109 32 uGQjww+cwaBN/09U9xrc5IaLzoA Fig. 5A ------- COMMENT: f5cfe860f60d0109 33 uGQjww+cwaBN/09U9xrc5IaLzoA Fig. 5A ------- COMMENT: f5cfe860f60d0109 34 uGQjww+cwaBN/09U9xrc5IaLzoA Fig. 5A ------- COMMENT: f5cfe860f60d0109 35 qWwtdsJsl6MXOYCoCBvr4MsMNhg Fig. 5B ------- COMMENT: f5cfe860f60d0109 39 qWwtdsJsl6MXOYCoCBvr4MsMNhg Fig. 5B ------- COMMENT: f5cfe860f60d0109 40 /xrHxpMt5fd65zyX44wOMb4CrV4 Fig. 5C ------- COMMENT: f5cfe860f60d0109 42 T0LM+p/HPg0kKNsZPBo09vwEmwc Fig. 6B, 6C ------- COMMENT: f5cfe860f60d0109 43 T0LM+p/HPg0kKNsZPBo09vwEmwc Fig. 6B, 6C ------- COMMENT: f5cfe860f60d0109 44 T0LM+p/HPg0kKNsZPBo09vwEmwc Fig. 6B, 6C ------- COMMENT: f5cfe860f60d0109 45 T0LM+p/HPg0kKNsZPBo09vwEmwc Fig. 6B, 6C ------- COMMENT: f5cfe860f60d0109 46 T0LM+p/HPg0kKNsZPBo09vwEmwc Fig. 6B, 6C ------- COMMENT: f5cfe860f60d0109 47 T0LM+p/HPg0kKNsZPBo09vwEmwc Fig. 6B, 6C ------- COMMENT: f5cfe860f60d0109 48 T0LM+p/HPg0kKNsZPBo09vwEmwc Fig. 6B, 6C ------- COMMENT: f5cfe860f60d0109 49 T0LM+p/HPg0kKNsZPBo09vwEmwc Fig. 6B, 6C ------- COMMENT: f5cfe860f60d0109 50 T0LM+p/HPg0kKNsZPBo09vwEmwc Fig. 6B, 6C ------- COMMENT: f5cfe860f60d0109 51 u4B0DbDyyUxOuutu8/72fBd69u8 Fig 6B, 6C ------- COMMENT: f5cfe860f60d0109 52 6hjPaQX/lBlGMkGrtZ8mCR+9zhk Fig. 6D ------- COMMENT: f5cfe860f60d0109 53 lLCy9zSWYqQgmtfKWx903QJhygo Fig. 6B ------- COMMENT: f5cfe860f60d0109 54 YKgGHCHIcRX8kvBh1X+7c9lNcuI Fig. 7B ------- COMMENT: f5cfe860f60d0109 55 YKgGHCHIcRX8kvBh1X+7c9lNcuI Fig. 7B ------- COMMENT: f5cfe860f60d0109 56 YKgGHCHIcRX8kvBh1X+7c9lNcuI Fig. 7B ------- COMMENT: f5cfe860f60d0109 57 QeBnF81pF95Ye64vOEM7w+BGXU8 Fig. 7C ------- COMMENT: f5cfe860f60d0109 58 YKgGHCHIcRX8kvBh1X+7c9lNcuI Fig. 7B ------- COMMENT: f5cfe860f60d0109 59 YKgGHCHIcRX8kvBh1X+7c9lNcuI Fig. 7B ------- COMMENT: f5cfe860f60d0109 60 QeBnF81pF95Ye64vOEM7w+BGXU8 Fig. 7C ------- COMMENT: f5cfe860f60d0109 61 B1e76LGhYsJIvOO15xyFTz1PH7A Fig 7D ------- COMMENT: f5cfe860f60d0109 62 yKshcWa36JVyzon7b5A8sSAsc+Y Fig. S2D ------- COMMENT: f5cfe860f60d0109 63 aJOnN6aaIYkecem8sLcfCWlmcMc Fig S2D ------- COMMENT: f5cfe860f60d0109 64 yKshcWa36JVyzon7b5A8sSAsc+Y Fig. S2D ------- COMMENT: f5cfe860f60d0109 65 yKshcWa36JVyzon7b5A8sSAsc+Y Fig. S2D ------- COMMENT: f5cfe860f60d0109 66 yKshcWa36JVyzon7b5A8sSAsc+Y Fig. S2D ------- COMMENT: f5cfe860f60d0109 67 yKshcWa36JVyzon7b5A8sSAsc+Y Fig. S2D ------- COMMENT: f5cfe860f60d0109 68 yKshcWa36JVyzon7b5A8sSAsc+Y Fig. S2D ------- COMMENT: f5cfe860f60d0109 76 GoOZFftAiBT1J6TJdA0TpL5pB1E Fig. 5H ------- COMMENT: f5cfe860f60d0109 77 WQLyl0//8P0VIIOSRekPWcPuOE8 Fig. 2C ------- COMMENT: f5cfe860f60d0109 78 arC3552MomYHro2b8CN7moCyf+w Video S2 ------- COMMENT: f5cfe860f60d0109 79 to1m2lA4f0bWJXIuBZXZ57r8XHM (comment: binds to the consensus sequence CCCCAY) (Fig. 4) ------- COMMENT: f5cfe860f60d0109 80 tWY+eV5TPGBy9/9JblVJfkaX3h4 Fig. 5A, 5B, 5C; Fig. 7C ------- COMMENT: f5cfe860f60d0109 81 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 82 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 83 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 84 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 85 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 86 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 87 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 88 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 89 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 90 woT0r01Z7iewDm9EpEXrKDGBLe0 Fig. 3D ------- COMMENT: f5cfe860f60d0109 92 06RGRv6eMFjM1IQPXon6kF19DP8 Fig 2B ------- COMMENT: f5cfe860f60d0109 93 06RGRv6eMFjM1IQPXon6kF19DP8 Fig 2B ------- COMMENT: f5cfe860f60d0109 94 WeK7yxLE1WYcbMMFfcCh4W0c0Ns Fig. 2D ------- COMMENT: f5cfe860f60d0109 95 to1m2lA4f0bWJXIuBZXZ57r8XHM (comment: binds to the consensus sequence CCCCAY) (Fig. 4) ------- COMMENT: f5dfe6e677d045d1 1 sPZQsR2ZRTVLfWSLkYwes289FIo constant throughout cell cycle ------- COMMENT: f5dfe6e677d045d1 2 /3WLRrsHa7gj+yvN+Z/liaRhTg8 (comment: higher affinity during S phase than G2/M) ------- COMMENT: f5dfe6e677d045d1 3 j3AzYQMJSRglidcy2511+Yo2ohg (comment: CHECK hyperphosphorylated in late S phase; phosphorylated on different sites in S versus G2/M) ------- COMMENT: f5f173fa6df509a1 1 ZytaQReDC04hlCV0q2elA9MFP2A (comment: Southern blot to detect telomeric sequence) ------- COMMENT: f5f173fa6df509a1 52 md79SElPV5onKb5SdMRPsD00x/I (comment: silencing normal as long as heterochromatin assembly can take place normally) ------- COMMENT: f5f173fa6df509a1 53 Lc3H63usqGgSpqngsr1nDXGEMKk (comment: experiment introduced otr::ura4+ in either a silenced state (from wild-type cells) or a desilenced state (from clr4delta cells) into poz1delta dcr1delta cells by genetic crosses) ------- COMMENT: f5f173fa6df509a1 159 ZytaQReDC04hlCV0q2elA9MFP2A (comment: Southern blot to detect telomeric sequence) ------- COMMENT: f5f173fa6df509a1 160 ZytaQReDC04hlCV0q2elA9MFP2A (comment: Southern blot to detect telomeric sequence) ------- COMMENT: f5f173fa6df509a1 161 ZytaQReDC04hlCV0q2elA9MFP2A (comment: Southern blot to detect telomeric sequence) ------- COMMENT: f5f173fa6df509a1 162 ZytaQReDC04hlCV0q2elA9MFP2A (comment: Southern blot to detect telomeric sequence) ------- COMMENT: f5f173fa6df509a1 163 ZytaQReDC04hlCV0q2elA9MFP2A (comment: Southern blot to detect telomeric sequence) ------- COMMENT: f5f173fa6df509a1 164 ZytaQReDC04hlCV0q2elA9MFP2A (comment: Southern blot to detect telomeric sequence) ------- COMMENT: f5f87c0cb83a3c7b 7 9uGTE+4JkNBq1UWg649P6+uXsiw (comment: requires long flap (binding affinity much higher with 27-nt than 15-nt flap)) ------- COMMENT: f5f87c0cb83a3c7b 41 9uGTE+4JkNBq1UWg649P6+uXsiw (comment: requires long flap (binding affinity much higher with 27-nt than 15-nt flap)) ------- COMMENT: f601538f3543ef0e 2 aj/otl3+Td5FTF2YU2fERwZavfU As shown in Fig. 3B, GFP- Pib2 was detectable on vacuolar membranes, which can be visualized by the FM4-64 dye, ------- COMMENT: f601538f3543ef0e 3 p23VaE2MmzhdxFHAPnFMA17hdOk Fig. 2A, 2B ------- COMMENT: f601538f3543ef0e 4 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: f601538f3543ef0e 5 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: f601538f3543ef0e 7 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: f601538f3543ef0e 8 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: f601538f3543ef0e 9 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: f601538f3543ef0e 10 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: f601538f3543ef0e 11 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: f601538f3543ef0e 12 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: f601538f3543ef0e 13 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: f601538f3543ef0e 14 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: f601538f3543ef0e 15 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: f601538f3543ef0e 16 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 17 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 18 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 19 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: f601538f3543ef0e 20 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: f601538f3543ef0e 21 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: f601538f3543ef0e 22 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: f601538f3543ef0e 23 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: f601538f3543ef0e 24 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: f601538f3543ef0e 25 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: f601538f3543ef0e 26 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: f601538f3543ef0e 27 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: f601538f3543ef0e 28 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: f601538f3543ef0e 29 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: f601538f3543ef0e 30 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 31 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 32 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 33 p23VaE2MmzhdxFHAPnFMA17hdOk Fig. 2A, 2B ------- COMMENT: f601538f3543ef0e 34 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: f601538f3543ef0e 37 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 38 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 41 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 42 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 43 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 44 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 45 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 46 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 47 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 48 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 49 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 50 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 51 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 52 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 53 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 54 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 55 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 56 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 57 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: f601538f3543ef0e 58 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: f601538f3543ef0e 59 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: f601538f3543ef0e 60 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f601538f3543ef0e 61 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: f64bdfbbde0df34c 1 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 2 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 3 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 4 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 5 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 6 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 7 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 8 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 9 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 10 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 11 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 12 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 13 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 14 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 15 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 16 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 17 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 18 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 19 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 20 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 21 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 22 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 23 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 24 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: f64bdfbbde0df34c 26 y9y2n1YJEMIYHGyK6ZxHYip81Yc Finally, Cter-GFP remained more con- centrated at the new tip compared to the old tip in short cells after sister cell separation. [...] An association with the cell tips and septum region was never observed for wild-type mid1p and may either result from the truncation of mid1p or from the higher concentration of this construct. ------- COMMENT: f64bdfbbde0df34c 27 zXfmiwcsxbaRgPHQhHISudqAluM Cter-GFP was then observed in the region of septum formation (Fig. 1B, 16 to 52 min), where it was probably associated with the ingressing plasma membrane of the cleavage furrow. [...] An association with the cell tips and septum region was never observed for wild-type mid1p and may either result from the truncation of mid1p or from the higher concentration of this construct. ------- COMMENT: f64bdfbbde0df34c 28 0k1exm+o2shm0Oo+Z9Vd9qISCR0 Cter-GFP mutated in the helix (Helix Cter-GFP) was concentrated in the nucleus, even during mitosis (arrow in Fig. 2C) ------- COMMENT: f64bdfbbde0df34c 29 IpttIPjqSgo/Z6vlldujRjkyung When mutations in the helix were combined with mutations in the NLS (Helix* NLS* Cter-GFP), Cter-GFP was found in the cytoplasm. Fig. 2C ------- COMMENT: f64bdfbbde0df34c 30 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: f64bdfbbde0df34c 31 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: f64bdfbbde0df34c 32 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: f64bdfbbde0df34c 33 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: f64bdfbbde0df34c 34 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: f64bdfbbde0df34c 35 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: f64bdfbbde0df34c 36 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: f64bdfbbde0df34c 37 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: f64bdfbbde0df34c 38 gRNX+fJlwjiVqyJxI4SSQXsufyw These observations indicate that Helix NLS mid1-mRFP colocalize with myo2-GFP and cdc12-GFP in early mitosis. This suggests that in wild-type cells mid1p mediates anchorage of myo2p and cdc12p to the central cortex in early mitosis. ------- COMMENT: f64bdfbbde0df34c 39 78rlxJ10ejGraFSWF8GUgjZUtJE Mass spectrometry analysis revealed that GST-mid1p eluate con- tained the kinase plo1p, ------- COMMENT: f64bdfbbde0df34c 40 6EXqH9GVDNGPX4IGXL7mAEfiCuA Together, these data indicate that mid1p is able to form oligomers in which molecules may adopt a parallel configuration ------- COMMENT: f68bbea7e639d8fd 13 WChsce2VRhzXt3lN8bhj/izI4iY (comment: enhanced long-range double strand DNA break resectioning defect) ------- COMMENT: f68bbea7e639d8fd 14 9nUPZ/RViDHlkoqOU2hV5DUWy7c (comment: rescue of double strand DNA break resection defect) ------- COMMENT: f68bbea7e639d8fd 15 9nUPZ/RViDHlkoqOU2hV5DUWy7c (comment: rescue of double strand DNA break resection defect) ------- COMMENT: f68bbea7e639d8fd 17 mS6hQaI1vglW6DdXpf+j/DurqUE (comment: rescue of decreased localization of ssb1 (RPA) to double strand DNA break) ------- COMMENT: f6960ee6a5709c02 1 bXMTWjjXI2jR4KQdXAu1uGxmPmw A Homodimer of the Mis18 C-Terminal Domain Interacts with a Mis16-Eic1 Heterodimer ------- COMMENT: f6960ee6a5709c02 3 8eaIyfxLrFo7PLXjNG2POwflS7c The Stoichiometry of the S. pombe Mis18 Holo-Complex Is (Mis16)2:(Eic1)2:(Mis18)4 T ------- COMMENT: f6960ee6a5709c02 4 8eaIyfxLrFo7PLXjNG2POwflS7c The Stoichiometry of the S. pombe Mis18 Holo-Complex Is (Mis16)2:(Eic1)2:(Mis18)4 T ------- COMMENT: f6960ee6a5709c02 5 8eaIyfxLrFo7PLXjNG2POwflS7c The Stoichiometry of the S. pombe Mis18 Holo-Complex Is (Mis16)2:(Eic1)2:(Mis18)4 T ------- COMMENT: f6960ee6a5709c02 6 r/vsiLKudZz7K9vjSB/MGea6Vc0 FIgure 4D ------- COMMENT: f6960ee6a5709c02 9 +E0E5wiBTB5Vk0mm0jPqjvakk7Q (Figures 6A, 6B). ------- COMMENT: f6960ee6a5709c02 10 uSi1vbHqK4d//P/AJuIX8318hGw However, the interaction is significantly diminished when either L32 (Mis16D1–32), or both L32 (Mis16D1–32) and L32/W33 (Mis16D1–33), are deleted (Figures 6A and 6B). In contrast, the Mis16-H4a1 interaction was only mildly affected in the context of the Mis16D1–33 truncation. These findings strongly suggest that Mis16 L32 and W33 participate in specific interactions with Eic1 but not histone H4. To verify these observations in vivo, we performed ------- COMMENT: f6960ee6a5709c02 11 uSi1vbHqK4d//P/AJuIX8318hGw However, the interaction is significantly diminished when either L32 (Mis16D1–32), or both L32 (Mis16D1–32) and L32/W33 (Mis16D1–33), are deleted (Figures 6A and 6B). In contrast, the Mis16-H4a1 interaction was only mildly affected in the context of the Mis16D1–33 truncation. These findings strongly suggest that Mis16 L32 and W33 participate in specific interactions with Eic1 but not histone H4. To verify these observations in vivo, we performed ------- COMMENT: f6960ee6a5709c02 12 uSi1vbHqK4d//P/AJuIX8318hGw However, the interaction is significantly diminished when either L32 (Mis16D1–32), or both L32 (Mis16D1–32) and L32/W33 (Mis16D1–33), are deleted (Figures 6A and 6B). In contrast, the Mis16-H4a1 interaction was only mildly affected in the context of the Mis16D1–33 truncation. These findings strongly suggest that Mis16 L32 and W33 participate in specific interactions with Eic1 but not histone H4. To verify these observations in vivo, we performed ------- COMMENT: f6960ee6a5709c02 13 uSi1vbHqK4d//P/AJuIX8318hGw However, the interaction is significantly diminished when either L32 (Mis16D1–32), or both L32 (Mis16D1–32) and L32/W33 (Mis16D1–33), are deleted (Figures 6A and 6B). In contrast, the Mis16-H4a1 interaction was only mildly affected in the context of the Mis16D1–33 truncation. These findings strongly suggest that Mis16 L32 and W33 participate in specific interactions with Eic1 but not histone H4. To verify these observations in vivo, we performed ------- COMMENT: f6960ee6a5709c02 14 uSi1vbHqK4d//P/AJuIX8318hGw However, the interaction is significantly diminished when either L32 (Mis16D1–32), or both L32 (Mis16D1–32) and L32/W33 (Mis16D1–33), are deleted (Figures 6A and 6B). In contrast, the Mis16-H4a1 interaction was only mildly affected in the context of the Mis16D1–33 truncation. These findings strongly suggest that Mis16 L32 and W33 participate in specific interactions with Eic1 but not histone H4. To verify these observations in vivo, we performed ------- COMMENT: f6a5a7da08695182 1 5DWqo03puFeQtodWqcCbmJ6nfyM fig 1A ------- COMMENT: f6a5a7da08695182 2 5DWqo03puFeQtodWqcCbmJ6nfyM fig 1A ------- COMMENT: f6a5a7da08695182 3 5DWqo03puFeQtodWqcCbmJ6nfyM fig 1A ------- COMMENT: f6a5a7da08695182 4 5DWqo03puFeQtodWqcCbmJ6nfyM fig 1A ------- COMMENT: f6a5a7da08695182 5 5DWqo03puFeQtodWqcCbmJ6nfyM fig 1A ------- COMMENT: f6a5a7da08695182 6 5DWqo03puFeQtodWqcCbmJ6nfyM fig 1A ------- COMMENT: f6a5a7da08695182 7 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: f6a5a7da08695182 8 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: f6a5a7da08695182 9 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: f6a5a7da08695182 10 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: f6a5a7da08695182 11 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: f6a5a7da08695182 12 +htt9lF8uPXDflnToWBhsLxcNC0 fig 1B ------- COMMENT: f6a5a7da08695182 13 GSDoKVHrCsiUO6zYP0Vg8a8VAAQ fig 1D ------- COMMENT: f6a5a7da08695182 14 GSDoKVHrCsiUO6zYP0Vg8a8VAAQ fig 1D ------- COMMENT: f6a5a7da08695182 15 I0eztaUetfv+tRt+spYJhW9cbeI (Figure 2A) ------- COMMENT: f6a5a7da08695182 16 I0eztaUetfv+tRt+spYJhW9cbeI (Figure 2A) ------- COMMENT: f6a5a7da08695182 17 hVBF0JlxBq71UOcxW8PFL8HBUQ0 (Figure 2B) ------- COMMENT: f6a5a7da08695182 18 7IzPVIuZhuKqIVjB4l/cExHiwb8 fig 2D ------- COMMENT: f6a5a7da08695182 19 sBNmtNgFvy88X/4RDO33gTgRnKw fig 2E ------- COMMENT: f6a5a7da08695182 20 9eVuVr7SyYYL493JmJJ0qXoaiM0 Figure 2E (comment: how is this abnormal? i got confused here) ------- COMMENT: f6a5a7da08695182 21 Mc1Dl/8ePjG+W3BQIevC1vaEvqw Figure 2D ------- COMMENT: f6a5a7da08695182 22 cWU1gxrBhSdpW25jqWFTdTZ9qBA fig 3A ------- COMMENT: f6a5a7da08695182 23 cWU1gxrBhSdpW25jqWFTdTZ9qBA fig 3A ------- COMMENT: f6a5a7da08695182 24 cWU1gxrBhSdpW25jqWFTdTZ9qBA fig 3A ------- COMMENT: f6a5a7da08695182 25 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: f6a5a7da08695182 26 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: f6a5a7da08695182 27 e5egf7nPDJfl2mpsJq+7xlwicY8 fig 3C ------- COMMENT: f6a5a7da08695182 29 9V3MxsMdUuHD2YnzquM3ZneMUHI Figure S3E ------- COMMENT: f6a5a7da08695182 30 9V3MxsMdUuHD2YnzquM3ZneMUHI Figure S3E ------- COMMENT: f6a5a7da08695182 31 9V3MxsMdUuHD2YnzquM3ZneMUHI Figure S3E ------- COMMENT: f6a5a7da08695182 34 +tpKqK7DKZFDQiw6HBFazt9u/IA Figures 4B, S4A ------- COMMENT: f6a5a7da08695182 35 +tpKqK7DKZFDQiw6HBFazt9u/IA Figures 4B, S4A ------- COMMENT: f6a5a7da08695182 37 I6FsM7K0a9xc/Lp4C0BJo9EHWZw (Figures 4C, S4B). (comment: non kinetochore bound) ------- COMMENT: f6a5a7da08695182 38 I6FsM7K0a9xc/Lp4C0BJo9EHWZw (Figures 4C, S4B). (comment: non kinetochore bound) ------- COMMENT: f6a5a7da08695182 39 TE/bDzpsWWSu/g91UxFWYPxzyUU ((Figure S4C)). (comment: non kinetochore bound) ------- COMMENT: f6a5a7da08695182 40 nMN/t9QIUj3xvll2mD0Mi8zNeV0 Together, these data indicate that Mph1 (Mps1) kinase and Dis2 (PP1) phosphatase antagonistically regulate the interaction of Mad1 and Mad2 with Bub1 in fission yeast, most likely through phosphorylation of the conserved central motif of Bub1. ------- COMMENT: f6a5a7da08695182 41 nMN/t9QIUj3xvll2mD0Mi8zNeV0 Together, these data indicate that Mph1 (Mps1) kinase and Dis2 (PP1) phosphatase antagonistically regulate the interaction of Mad1 and Mad2 with Bub1 in fission yeast, most likely through phosphorylation of the conserved central motif of Bub1. ------- COMMENT: f6b6af4b58d157ce 1 mJ50UfnNPHLhcrbJXCZL/jyugc8 Fig1C ii ------- COMMENT: f6b6af4b58d157ce 2 VehkDxzm7Earh2/xcgG7+qWZ2Yw Fig1C iii ------- COMMENT: f6b6af4b58d157ce 3 VehkDxzm7Earh2/xcgG7+qWZ2Yw Fig1C iii ------- COMMENT: f6b6af4b58d157ce 4 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: f6b6af4b58d157ce 5 J0gRmoDzBc52fRYy+iELsL4OngE data not shown. 95% of cells re bent or T shaped but don't say the different percentages so I've left it as bent ------- COMMENT: f6b6af4b58d157ce 6 60+4zbo1VVS9zvWbd/RHvmPoavE data not shown. tea1 on multi copy plasmid -R2 suppresses the cell shape defect of tea1 delta ------- COMMENT: f6b6af4b58d157ce 7 +FdG1EhV3xb42GERCpID2Wl4dC0 Fig2B, 2C ------- COMMENT: f6b6af4b58d157ce 8 +ABQaifSS/9JzdCSNlh4clPg9Y4 Fig 2B, 2C ------- COMMENT: f6b6af4b58d157ce 9 eTkUGsRQ1d7a/fmlA3oK3ttibXY Fig 2C protein localised to both cell tips ------- COMMENT: f6b6af4b58d157ce 10 f4UW3RBCAC6PCoeeXfKoJIK8DaI Fig 2C Protein localised to both cell tips during monopolar growth ------- COMMENT: f6b6af4b58d157ce 11 8AtlTSV0VXAgz/6n4R9V088EK/0 Fig 2D Protein localised to both cell tips during monopolar growth ------- COMMENT: f6b6af4b58d157ce 12 oT/OcXiSsH6RWhVLJJEUtUifcUE Fig3A ------- COMMENT: f6b6af4b58d157ce 13 oT/OcXiSsH6RWhVLJJEUtUifcUE Fig3A ------- COMMENT: f6b6af4b58d157ce 14 73t/hLdCg2aapNrKctGyrt0oomk Fig4A ------- COMMENT: f6b6af4b58d157ce 15 73t/hLdCg2aapNrKctGyrt0oomk Fig4A ------- COMMENT: f6b6af4b58d157ce 16 73t/hLdCg2aapNrKctGyrt0oomk Fig4A ------- COMMENT: f6b6af4b58d157ce 17 73t/hLdCg2aapNrKctGyrt0oomk Fig4A ------- COMMENT: f6b6af4b58d157ce 18 Ox/O5fcFkdYZstttnXOWSkqFbRY Fig5 (comment: shown using TBZ treatment and wash out and by cold shock and relocalization) ------- COMMENT: f6b6af4b58d157ce 19 REqA86wgEckE1+po1IZQ8wC5RUg Fig5C ------- COMMENT: f6b6af4b58d157ce 20 REqA86wgEckE1+po1IZQ8wC5RUg Fig5C ------- COMMENT: f6b6af4b58d157ce 21 REqA86wgEckE1+po1IZQ8wC5RUg Fig5C ------- COMMENT: f6b6af4b58d157ce 22 ueWuePuKUArPylMdBi9/uWS/LAc Fig5C (comment: cells blocked in mitosis so have no interphase MTs) ------- COMMENT: f6b6af4b58d157ce 24 FzPW38FvaNYcNujiUEn6O/THKyM Fig 6D ------- COMMENT: f6b6af4b58d157ce 29 M9juvCneX0OJZCB2t0ZBjwYHP+4 Fig6D ------- COMMENT: f6b6af4b58d157ce 30 mJ50UfnNPHLhcrbJXCZL/jyugc8 Fig1C ii ------- COMMENT: f6b6af4b58d157ce 33 VehkDxzm7Earh2/xcgG7+qWZ2Yw Fig1C iii ------- COMMENT: f73ec44b6db23cab 1 47wNig6ukk4Tkm2g0kY6TDzesVI C11-F32S was more active in yJI1 than in yYH1, while wild-type C11 had no effect (Sector 4). ------- COMMENT: f73ec44b6db23cab 3 VC/4AHunhfZO6Tcm0K1sKCQ1YsA Rpc2-T455I was clearly more active (white) in yJI1 than in yYH1, consistent with dT(6) readthrough as expected. ------- COMMENT: f76dbfe807197d79 1 oj6Zc0bGlFedEl9nQYItwqKoc7U Ofd1 was enriched in the nucleus with little cytosolic staining consistent with previous findings (Fig. 3A)( ------- COMMENT: f76dbfe807197d79 2 oj6Zc0bGlFedEl9nQYItwqKoc7U Ofd1 was enriched in the nucleus with little cytosolic staining consistent with previous findings (Fig. 3A)( ------- COMMENT: f76dbfe807197d79 3 Svre13UbJ6OlOYRC0ApZP5fpayk In sre1N nro1Δ cells, Ofd1 showed diffuse cytosolic staining indicating that Nro1 is required for Ofd1 nuclear localization. Staining for Ofd1 was specific as ofd1Δ cells showed no signal. Together, these results show that nuclear localization of Ofd1 is linked to Nro1. ------- COMMENT: f76dbfe807197d79 5 aBSOuEUdiDAMeUpBKP89hgi1MmM (comment: CHECK with ofd1) ------- COMMENT: f76dbfe807197d79 6 oxJAxN/vOgxqx4beSaBdYA8O/7U (comment: CHECK sre1 n terminus isoform***********)In nro1Δ cells with Ofd1 retained in the cytosol due to the loss of Nro1-mediated nuclear localization, Sre1N failed to accumulate in anaerobic conditions (Fig. 4B, lanes 7–9). ------- COMMENT: f76dbfe807197d79 7 0krNpXWzpmOpKgFIZGiP12CjNAE (comment: CHECK sre1 n terminus isoform***********.) As previously reported, Sre1N accumulated in sre1N cells under anaerobic conditions (Fig. 4B, lanes 1–3)(Lee et al., 2009). ------- COMMENT: f76dbfe807197d79 8 80kwvK2l0Mop4LuYTkwOgYq2mkk (comment: CHECK inhibitor GO:0031543 peptidyl-proline dioxygenase activity) Importantly, Sre1N also failed to accumulate in NLS-ofd1 nro1Δ cells in the absence of oxygen despite the restored Ofd1 nuclear localization (Fig. 4B, lanes 10–12). Taken together, these data support previous studies and demonstrate that Nro1 functions as a direct inhibitor of Ofd1 in Sre1N ------- COMMENT: f7872927fe35893f 1 DrPaD4svTJxwSPVlGKMVXtpdV1U Figure S5A, S5B, ------- COMMENT: f7872927fe35893f 2 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: f7872927fe35893f 3 eSbqZW0i+wucWTL2opV34hVR9s0 (comment: CHECK in H2O2) ------- COMMENT: f7872927fe35893f 4 eSbqZW0i+wucWTL2opV34hVR9s0 (comment: CHECK in H2O2) ------- COMMENT: f7872927fe35893f 5 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: f7872927fe35893f 6 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: f7872927fe35893f 7 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: f7872927fe35893f 8 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: f7872927fe35893f 9 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: f7872927fe35893f 10 3KUnvJa9gS8JPddMzu/PHfQMl8s Fig S5C, S6 ------- COMMENT: f78a781e3e01fdf4 2 8jTjtkNnNoFhiY57qk4SkW6skr0 figure 1A ------- COMMENT: f78a781e3e01fdf4 4 910eACewifs4esy1p4l45ztnaBA figure 1B (comment: plus end) ------- COMMENT: f78a781e3e01fdf4 9 8jTjtkNnNoFhiY57qk4SkW6skr0 figure 1A ------- COMMENT: f78a781e3e01fdf4 11 910eACewifs4esy1p4l45ztnaBA figure 1B (comment: plus end) ------- COMMENT: f79afeafbdf2d0ec 33 FSJ2xmnr6BeMUwmuqgsBeQE4SS0 (comment: evidence: immunoblot using antibody that recognizes thymine dimers) ------- COMMENT: f7a5828208a1fb1f 1 +Uo/dVVJaQHvzbN+pjYsdyw5noE from materials and methods ------- COMMENT: f7a5828208a1fb1f 2 +Uo/dVVJaQHvzbN+pjYsdyw5noE from materials and methods ------- COMMENT: f7a5828208a1fb1f 3 +Uo/dVVJaQHvzbN+pjYsdyw5noE from materials and methods ------- COMMENT: f7a5828208a1fb1f 4 +Uo/dVVJaQHvzbN+pjYsdyw5noE from materials and methods ------- COMMENT: f7a6b2bfb508bcb4 1 CmJe9ILz7aVgXXthBLeytPSkMPg The pull-down assay revealed that GST- SpSpo13F79A still bound to the nucleotide-free form of ScSec4 but at a lower level than wild-type GST-SpSpo13 (Figure 5A). In the GEF assay, adding GST-SpSpo13F79A did not stimulate mant-GDP dissociation, indicating that the mutant protein has lost GEF activity in vitro (Figure 5B). ------- COMMENT: f7a6b2bfb508bcb4 2 4yzRjFA+ohzfZdJoFs/93Qe0sKs Spo13-mRFP was visible at the SPB, beginning with cells in meiosis I and persisted at the SPB throughout meiosis II, as reported previously (Nakase et al., 2008; Figure 6B). ------- COMMENT: f7a6b2bfb508bcb4 3 4yzRjFA+ohzfZdJoFs/93Qe0sKs Spo13-mRFP was visible at the SPB, beginning with cells in meiosis I and persisted at the SPB throughout meiosis II, as reported previously (Nakase et al., 2008; Figure 6B). ------- COMMENT: f7a6b2bfb508bcb4 4 O2YIcsF+NnMl4eOIKPl57o9Tvsg The localization of SpSpo13F79A-mRFP was indistinguishable from the wild- type protein (Figure 6C), indicating that the mutant protein is expressed and properly localized. ------- COMMENT: f7a6b2bfb508bcb4 5 GPwm/vODJU7fxlyjFOSHgDjtvoo . Grow- ing FSMs were observed adjacent to each of the SPBs in 100% of the cells expressing wild-type Spspo13 (n  20), but no FSMs were formed in cells expressing Spspo13-F79A (Fig- ure 7) (n  20). The GEF activity of SpSpo13 is therefore required for FSM assembly in S. pombe. ------- COMMENT: f7a6b2bfb508bcb4 6 A99HsT8bTWAD2NubhPhkTnJlrSk A 6XHis-tagged version of SpYpt2 was purified from E. coli, and the ability of SpSpo13 to stimulate GDP release was examined (Figure 8A). Similar to what was observed with ScSec4 as a substrate (Figure 4B), SpSpo13 stimulated GDP release, although not as efficiently as Sc- Sec2. Thus, SpSpo13 can act on SpYpt2. ------- COMMENT: f7a6b2bfb508bcb4 7 A99HsT8bTWAD2NubhPhkTnJlrSk A 6XHis-tagged version of SpYpt2 was purified from E. coli, and the ability of SpSpo13 to stimulate GDP release was examined (Figure 8A). Similar to what was observed with ScSec4 as a substrate (Figure 4B), SpSpo13 stimulated GDP release, although not as efficiently as Sc- Sec2. Thus, SpSpo13 can act on SpYpt2. ------- COMMENT: f7d320e81fb9bd5f 8 ECJ3uzVVnwO8BRRGmeK+5KEdVjo (comment: CHECK activated_by(CHEBI:63041)) ------- COMMENT: f7db522593008e9c 5 ZgKU+ttzdQmFfrySydRkNJfZcDQ decreased local concentration of the myosin-II ------- COMMENT: f7db522593008e9c 6 ZgKU+ttzdQmFfrySydRkNJfZcDQ decreased local concentration of the myosin-II ------- COMMENT: f7db522593008e9c 7 +fje97q2SosU9bUU0QZIlRVkll4 localization of the myosin-II is abolished ------- COMMENT: f7db522593008e9c 8 MqtYBDefcj35MH7chjSYsasUMGI increased local concentration of the myosin-II ------- COMMENT: f7db522593008e9c 9 +quk0yVvYVKTDKC03KNaOZa59/8 large portion of the mutant forms cytoplasmic dots ------- COMMENT: f7e3367c6996a6f9 7 xVb6K9IUUZ6lKd1LVsOHIZII9PY inferred from combination of FYPO:0005798 and FYPO:0005828 ------- COMMENT: f7e6c33889ea1fa0 6 4RQDmWSGPAAJ7P+zTNwtjZKHODE (comment: CHECK during metaphase) ------- COMMENT: f7e6c33889ea1fa0 20 jUQ0+MZVMgglZtt5ZdWbmDUKia0 Thus, we conclude that Cdc2 activity prevents precocious localization of Mde4 to the metaphase spindle. ------- COMMENT: f836ef569847294d 1 CMUcW89zrVfJjwCdPZDn2qP4jok Figure 1. Cells with leucine auxotrophy (leu1-32 strain) show weak growth on the synthetic medium EMM supplemented with 0.2 mM leucine (Fig. 1B). EMM contains 0.5% NH4Cl ------- COMMENT: f836ef569847294d 2 rkUohM+T5kzjzwgbttmhdEAYNIU data not shown, related data in Figure 2A ------- COMMENT: f836ef569847294d 3 VHIs++3GkuObvAwn0PdlEOG98nw Figure 4 In 3.0% NH4Cl medium, the growth speed of both strains became slower after exposure to the high NH4Cl condition for 14 h. ------- COMMENT: f836ef569847294d 4 dYnLbZa+A6ws+3p+wPYCksORSTk Figure 3, this phenotype was observed for cat1_delta leu1-32 double mutant. ------- COMMENT: f83f9b9d22696ff4 1 3cYe5+7Z++eRZf8lH+hberChE4U We found that malonyl-mimetic K119D and K119E mutants showed sensitivity to a microtubule depolymerizing agent (TBZ) in the same way the H2A-S121A mutant had (Fig. S3B), ------- COMMENT: f83f9b9d22696ff4 2 3cYe5+7Z++eRZf8lH+hberChE4U We found that malonyl-mimetic K119D and K119E mutants showed sensitivity to a microtubule depolymerizing agent (TBZ) in the same way the H2A-S121A mutant had (Fig. S3B), ------- COMMENT: f83f9b9d22696ff4 3 3cYe5+7Z++eRZf8lH+hberChE4U We found that malonyl-mimetic K119D and K119E mutants showed sensitivity to a microtubule depolymerizing agent (TBZ) in the same way the H2A-S121A mutant had (Fig. S3B), ------- COMMENT: f83f9b9d22696ff4 4 3cYe5+7Z++eRZf8lH+hberChE4U We found that malonyl-mimetic K119D and K119E mutants showed sensitivity to a microtubule depolymerizing agent (TBZ) in the same way the H2A-S121A mutant had (Fig. S3B), ------- COMMENT: f83f9b9d22696ff4 5 rpRwXhm1unSOchkcLvnfA3H10Ek Intriguingly, we found that both Sgo2 centromeric localization during mitosis and Sgo1 centromeric localization during meiosis I were dramatically delocalized in the K119D and K119E mutants as well as the H2A-S121A mutant (Fig. 3C–E). ------- COMMENT: f83f9b9d22696ff4 6 rpRwXhm1unSOchkcLvnfA3H10Ek Intriguingly, we found that both Sgo2 centromeric localization during mitosis and Sgo1 centromeric localization during meiosis I were dramatically delocalized in the K119D and K119E mutants as well as the H2A-S121A mutant (Fig. 3C–E). ------- COMMENT: f83f9b9d22696ff4 7 rpRwXhm1unSOchkcLvnfA3H10Ek Intriguingly, we found that both Sgo2 centromeric localization during mitosis and Sgo1 centromeric localization during meiosis I were dramatically delocalized in the K119D and K119E mutants as well as the H2A-S121A mutant (Fig. 3C–E). ------- COMMENT: f83f9b9d22696ff4 8 rpRwXhm1unSOchkcLvnfA3H10Ek Intriguingly, we found that both Sgo2 centromeric localization during mitosis and Sgo1 centromeric localization during meiosis I were dramatically delocalized in the K119D and K119E mutants as well as the H2A-S121A mutant (Fig. 3C–E). ------- COMMENT: f83f9b9d22696ff4 9 rpRwXhm1unSOchkcLvnfA3H10Ek Intriguingly, we found that both Sgo2 centromeric localization during mitosis and Sgo1 centromeric localization during meiosis I were dramatically delocalized in the K119D and K119E mutants as well as the H2A-S121A mutant (Fig. 3C–E). ------- COMMENT: f83f9b9d22696ff4 10 rpRwXhm1unSOchkcLvnfA3H10Ek Intriguingly, we found that both Sgo2 centromeric localization during mitosis and Sgo1 centromeric localization during meiosis I were dramatically delocalized in the K119D and K119E mutants as well as the H2A-S121A mutant (Fig. 3C–E). ------- COMMENT: f83f9b9d22696ff4 11 rpRwXhm1unSOchkcLvnfA3H10Ek Intriguingly, we found that both Sgo2 centromeric localization during mitosis and Sgo1 centromeric localization during meiosis I were dramatically delocalized in the K119D and K119E mutants as well as the H2A-S121A mutant (Fig. 3C–E). ------- COMMENT: f83f9b9d22696ff4 12 rpRwXhm1unSOchkcLvnfA3H10Ek Intriguingly, we found that both Sgo2 centromeric localization during mitosis and Sgo1 centromeric localization during meiosis I were dramatically delocalized in the K119D and K119E mutants as well as the H2A-S121A mutant (Fig. 3C–E). ------- COMMENT: f83f9b9d22696ff4 13 rpRwXhm1unSOchkcLvnfA3H10Ek Intriguingly, we found that both Sgo2 centromeric localization during mitosis and Sgo1 centromeric localization during meiosis I were dramatically delocalized in the K119D and K119E mutants as well as the H2A-S121A mutant (Fig. 3C–E). ------- COMMENT: f83f9b9d22696ff4 14 rpRwXhm1unSOchkcLvnfA3H10Ek Intriguingly, we found that both Sgo2 centromeric localization during mitosis and Sgo1 centromeric localization during meiosis I were dramatically delocalized in the K119D and K119E mutants as well as the H2A-S121A mutant (Fig. 3C–E). ------- COMMENT: f83f9b9d22696ff4 15 rpRwXhm1unSOchkcLvnfA3H10Ek Intriguingly, we found that both Sgo2 centromeric localization during mitosis and Sgo1 centromeric localization during meiosis I were dramatically delocalized in the K119D and K119E mutants as well as the H2A-S121A mutant (Fig. 3C–E). ------- COMMENT: f83f9b9d22696ff4 16 rpRwXhm1unSOchkcLvnfA3H10Ek Intriguingly, we found that both Sgo2 centromeric localization during mitosis and Sgo1 centromeric localization during meiosis I were dramatically delocalized in the K119D and K119E mutants as well as the H2A-S121A mutant (Fig. 3C–E). ------- COMMENT: f83f9b9d22696ff4 17 PX72wStan7jRc5ToQ/tatrjEngc Consistently, sister chromatid non-disjunction at meiosis II was signifcantly increased in the K119D and K119E mutants ------- COMMENT: f83f9b9d22696ff4 18 PX72wStan7jRc5ToQ/tatrjEngc Consistently, sister chromatid non-disjunction at meiosis II was signifcantly increased in the K119D and K119E mutants ------- COMMENT: f83f9b9d22696ff4 19 PX72wStan7jRc5ToQ/tatrjEngc Consistently, sister chromatid non-disjunction at meiosis II was signifcantly increased in the K119D and K119E mutants ------- COMMENT: f83f9b9d22696ff4 20 PX72wStan7jRc5ToQ/tatrjEngc Consistently, sister chromatid non-disjunction at meiosis II was signifcantly increased in the K119D and K119E mutants ------- COMMENT: f83f9b9d22696ff4 21 mHyTIr26+4bL1CgQULxNVP10gNk Consistently, sister chromatid non-disjunction at meiosis II was signifcantly increased in the K119D and K119E mutants as well as the H2A-S121A mutant (Fig. S3D). ------- COMMENT: f83f9b9d22696ff4 22 mHyTIr26+4bL1CgQULxNVP10gNk Consistently, sister chromatid non-disjunction at meiosis II was signifcantly increased in the K119D and K119E mutants as well as the H2A-S121A mutant (Fig. S3D). ------- COMMENT: f83f9b9d22696ff4 23 O+LfsUoLgL/GOMIXmsJYDSFLyzU Te recombinant proteins of fssion yeast H2A (SpHta1) were also purifed. An in vitro kinase assay using these recombinant proteins showed that the H2A-K119D and the H2A-K119E mutants, as well as the H2A-S121A mutant, were not signifcantly phosphorylated by Bub1, whereas H2A-K119R mutant proteins were phosphorylated by Bub1 (Fig. 4B). ------- COMMENT: f83f9b9d22696ff4 25 O+LfsUoLgL/GOMIXmsJYDSFLyzU Te recombinant proteins of fssion yeast H2A (SpHta1) were also purifed. An in vitro kinase assay using these recombinant proteins showed that the H2A-K119D and the H2A-K119E mutants, as well as the H2A-S121A mutant, were not signifcantly phosphorylated by Bub1, whereas H2A-K119R mutant proteins were phosphorylated by Bub1 (Fig. 4B). ------- COMMENT: f83f9b9d22696ff4 26 O+LfsUoLgL/GOMIXmsJYDSFLyzU Te recombinant proteins of fssion yeast H2A (SpHta1) were also purifed. An in vitro kinase assay using these recombinant proteins showed that the H2A-K119D and the H2A-K119E mutants, as well as the H2A-S121A mutant, were not signifcantly phosphorylated by Bub1, whereas H2A-K119R mutant proteins were phosphorylated by Bub1 (Fig. 4B). ------- COMMENT: f83f9b9d22696ff4 27 O+LfsUoLgL/GOMIXmsJYDSFLyzU Te recombinant proteins of fssion yeast H2A (SpHta1) were also purifed. An in vitro kinase assay using these recombinant proteins showed that the H2A-K119D and the H2A-K119E mutants, as well as the H2A-S121A mutant, were not signifcantly phosphorylated by Bub1, whereas H2A-K119R mutant proteins were phosphorylated by Bub1 (Fig. 4B). ------- COMMENT: f83f9b9d22696ff4 28 O+LfsUoLgL/GOMIXmsJYDSFLyzU Te recombinant proteins of fssion yeast H2A (SpHta1) were also purifed. An in vitro kinase assay using these recombinant proteins showed that the H2A-K119D and the H2A-K119E mutants, as well as the H2A-S121A mutant, were not signifcantly phosphorylated by Bub1, whereas H2A-K119R mutant proteins were phosphorylated by Bub1 (Fig. 4B). ------- COMMENT: f83f9b9d22696ff4 29 O+LfsUoLgL/GOMIXmsJYDSFLyzU Te recombinant proteins of fssion yeast H2A (SpHta1) were also purifed. An in vitro kinase assay using these recombinant proteins showed that the H2A-K119D and the H2A-K119E mutants, as well as the H2A-S121A mutant, were not signifcantly phosphorylated by Bub1, whereas H2A-K119R mutant proteins were phosphorylated by Bub1 (Fig. 4B). ------- COMMENT: f83f9b9d22696ff4 30 O+LfsUoLgL/GOMIXmsJYDSFLyzU Te recombinant proteins of fssion yeast H2A (SpHta1) were also purifed. An in vitro kinase assay using these recombinant proteins showed that the H2A-K119D and the H2A-K119E mutants, as well as the H2A-S121A mutant, were not signifcantly phosphorylated by Bub1, whereas H2A-K119R mutant proteins were phosphorylated by Bub1 (Fig. 4B). ------- COMMENT: f83f9b9d22696ff4 31 cpClRucaeMPbAIlhD0oOxTd+PFw An acetyl-mimetic H2A-K119Q mutation slightly inhibited Bub1-mediated H2A phosphorylation (Fig. 4B) ------- COMMENT: f83f9b9d22696ff4 32 cpClRucaeMPbAIlhD0oOxTd+PFw An acetyl-mimetic H2A-K119Q mutation slightly inhibited Bub1-mediated H2A phosphorylation (Fig. 4B) ------- COMMENT: f85ac1dcd8ab8c25 1 aZ/a63TL8DAUQ9gUQiEw9J9xGx8 (comment: I changed this to exocytosis. This is required for cell wall organization, as it is causally upstream (val)) ------- COMMENT: f85ac1dcd8ab8c25 44 BXfSUqbbbW1sdCReo5wtA21/7Dw effect on secretion is specific for cell wall enzymes; secretion of acid phosphatase is normal (comment: but assayed acid phosphatase activity in medium, so can't tell which gene(s)) ------- COMMENT: f85b178fd2fdb4a0 5 d0ZmZjtPuBYY7Gt81QTNFxL7xzY Fig. 1d (comment: vw: localized by the secretory pathway) ------- COMMENT: f85b178fd2fdb4a0 7 CFnQxPG6p9ywfLGuxKEmPsOSKeQ Fig. 1e ------- COMMENT: f85b178fd2fdb4a0 23 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: f85b178fd2fdb4a0 31 /ChKLnjfSaPsDfyP4sFkeAOTl40 Fig. 5c ------- COMMENT: f85b178fd2fdb4a0 32 2i8uVaiNJwxTe67V4m9IqGyReC8 fig 5 ------- COMMENT: f86a4c2ed458dac5 1 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: f86a4c2ed458dac5 2 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: f86a4c2ed458dac5 3 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: f86a4c2ed458dac5 4 ILpDh7CKek1WhWQUGFO27I4L52Y Fig. 1C ------- COMMENT: f86a4c2ed458dac5 5 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: f86a4c2ed458dac5 6 I2WhMrTu6OJ5lxErfVsrXKDcslA Fig. 2 ------- COMMENT: f86a4c2ed458dac5 7 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: f86a4c2ed458dac5 8 tQHdVWiM89WAqMxgh38u4AFv9JQ Nonetheless, taken together, our results are consistent with the 93–100-amino acid region of Php4 functioning as an NES in S. pombe. ------- COMMENT: f86a4c2ed458dac5 9 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: f86a4c2ed458dac5 11 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: f86a4c2ed458dac5 12 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: f86a4c2ed458dac5 13 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: f86a4c2ed458dac5 14 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: f86a4c2ed458dac5 15 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: f86a4c2ed458dac5 16 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: f86a4c2ed458dac5 17 lt2pkx5XPTUurrYIIsqW8bTRKF8 Fig. 7C ------- COMMENT: f86a4c2ed458dac5 18 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: f86a4c2ed458dac5 19 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: f86a4c2ed458dac5 20 FkWjCHPXelPQLtr1AkZdsc+Y128 Fig. 9A ------- COMMENT: f86a4c2ed458dac5 21 FkWjCHPXelPQLtr1AkZdsc+Y128 Fig. 9A ------- COMMENT: f86a4c2ed458dac5 22 FkWjCHPXelPQLtr1AkZdsc+Y128 Fig. 9A ------- COMMENT: f86a4c2ed458dac5 23 FkWjCHPXelPQLtr1AkZdsc+Y128 Fig. 9A ------- COMMENT: f86a4c2ed458dac5 24 FkWjCHPXelPQLtr1AkZdsc+Y128 Fig. 9A ------- COMMENT: f86a4c2ed458dac5 25 FkWjCHPXelPQLtr1AkZdsc+Y128 Fig. 9A ------- COMMENT: f895d3b39398c5aa 1 BJ79CCdIs9uCpO29r+29Uejo1Lo Fig. 1A ------- COMMENT: f895d3b39398c5aa 2 BJ79CCdIs9uCpO29r+29Uejo1Lo Fig. 1A ------- COMMENT: f895d3b39398c5aa 3 BJ79CCdIs9uCpO29r+29Uejo1Lo Fig. 1A ------- COMMENT: f895d3b39398c5aa 4 gC+OPtCC43GyDCtaG9nh6Kn4Rmc Figure S1C ------- COMMENT: f895d3b39398c5aa 5 gC+OPtCC43GyDCtaG9nh6Kn4Rmc Figure S1C ------- COMMENT: f895d3b39398c5aa 6 9LIBPcMjihWY9sUg9qblKWKB2aQ The FISH data revealed that telomere clustering was not affected by pku70Δ and pku80Δ, but telomere tethering to the nuclear periphery was significantly compromised by pkuΔ (P < 0.001, Mann-Whitney U test), suggesting that telomere clustering and tethering to the nuclear periphery are distinct processes (Figures S2A and S2B) ------- COMMENT: f895d3b39398c5aa 7 sxAmExRZTxm64wlYsaBQLyuLPBM (Figures S2A, S2B) ------- COMMENT: f895d3b39398c5aa 8 iUKMvEOeGeFTahN5FXz8K237UsI (Figures S2A, S2B). AND As previously shown, telomere tethering was significantly compromised in rap1Δ and bqt4Δ cells (Figure S2C; Chikashige et al., 2009). ------- COMMENT: f895d3b39398c5aa 10 mBLh+HT6rVloXbgoh68tXkUqvCs As previously shown, telomere tethering was significantly compromised in rap1Δ and bqt4Δ cells (Figure S2C; Chikashige et al., 2009). ------- COMMENT: f895d3b39398c5aa 12 mBLh+HT6rVloXbgoh68tXkUqvCs As previously shown, telomere tethering was significantly compromised in rap1Δ and bqt4Δ cells (Figure S2C; Chikashige et al., 2009). ------- COMMENT: f895d3b39398c5aa 13 JY+2tw2LHcMCgkHo3J3N4cRkVUE Interestingly, Tf clustering was impaired by pku70Δ and pku80Δ at a level similar to that observed in abp1Δ cells (Figure 2A). ------- COMMENT: f895d3b39398c5aa 14 JY+2tw2LHcMCgkHo3J3N4cRkVUE Interestingly, Tf clustering was impaired by pku70Δ and pku80Δ at a level similar to that observed in abp1Δ cells (Figure 2A). ------- COMMENT: f895d3b39398c5aa 15 JY+2tw2LHcMCgkHo3J3N4cRkVUE Interestingly, Tf clustering was impaired by pku70Δ and pku80Δ at a level similar to that observed in abp1Δ cells (Figure 2A). ------- COMMENT: f895d3b39398c5aa 17 sXTCFctEWF3tZI3o64F4BwVwf/k clustering and tethering of centromeres to the nuclear periphery were not affected in pku70Δ and pku80Δ cells, although Ku does localize at centromeres (Figures 1E, 3A, and 3B) ------- COMMENT: f895d3b39398c5aa 18 sXTCFctEWF3tZI3o64F4BwVwf/k clustering and tethering of centromeres to the nuclear periphery were not affected in pku70Δ and pku80Δ cells, although Ku does localize at centromeres (Figures 1E, 3A, and 3B) ------- COMMENT: f895d3b39398c5aa 19 GJ+YqZ9tQodEdHs9jdPU/i9MjEE (comment: NOTE IS STILL LOCALIZED TO NUCLEAR ERIPHERY) Remarkably, the association of Tf elements with centromeres was significantly compromised in pkuΔ cells (P < 0.00001, Mann-Whitney U test; Figures 3D and 3E). ------- COMMENT: f895d3b39398c5aa 20 GJ+YqZ9tQodEdHs9jdPU/i9MjEE (comment: NOTE IS STILL LOCALIZED TO NUCLEAR ERIPHERY) Remarkably, the association of Tf elements with centromeres was significantly compromised in pkuΔ cells (P < 0.00001, Mann-Whitney U test; Figures 3D and 3E). ------- COMMENT: f895d3b39398c5aa 21 KdTbcoro4NG/ev33YoPc8SGW7H8 We found that Tf clustering and the association of Tf cluster with centromeres were significantly compromised in the cut14-208 condensin mutant Figures 4A and 4B), ------- COMMENT: f895d3b39398c5aa 22 KdTbcoro4NG/ev33YoPc8SGW7H8 We found that Tf clustering and the association of Tf cluster with centromeres were significantly compromised in the cut14-208 condensin mutant Figures 4A and 4B), ------- COMMENT: f895d3b39398c5aa 23 H83PiCyOi6lx0tiVPk9e7C4J8Nc Figures 6A, 6B hst4Δ and clr6-1 HDAC mutations, but not other HDAC mutations, significantly compromised Tf clustering and the association of Tf cluster with centromeres; Figures 6A and 6B) ------- COMMENT: f895d3b39398c5aa 24 I13VGfNrwvn47i3rv5nfEAnz1ZU Figures 6A, 6B), (comment: see above) ------- COMMENT: f895d3b39398c5aa 25 zCLkjbf1TyQAiYgZjq4fLvGHjNo Figures 6A, 6B) (comment: see above) ------- COMMENT: f895d3b39398c5aa 26 zCLkjbf1TyQAiYgZjq4fLvGHjNo Figures 6A, 6B) (comment: see above) ------- COMMENT: f895d3b39398c5aa 27 uH3FaerdXQ95B6lxaNvx5vgGLVI Moreover, only the rtt109Δ HAT mutations, but not other HAT mutations, significantly promoted the association of Tf cluster with centromeres, whereas none of the HAT mutations affected Tf clustering (Figures 6C and 6D). ------- COMMENT: f895d3b39398c5aa 28 hoWo0we8nPEDZsixmvJYrn7Wf4A Figure 6E). H3K56 acetylation antagonizes Tf clustering at centromeres. binding of Ku was reduced and enhanced in hst4Δ and rtt109Δ cells, respectively, ------- COMMENT: f895d3b39398c5aa 29 mFozPyMlZkzNadv/nITb5q00cFA Figure 6E). H3K56 acetylation antagonizes Tf clustering at centromeres. binding of Ku was reduced and enhanced in hst4Δ and rtt109Δ cells, respectivelyWe examined how H3K56 acetylation antagonizes Tf clustering at centromeres. Remarkably, binding of Ku was reduced and enhanced in hst4Δ and rtt109Δ cells, respectively, suggesting that H3K56 acetylation has an inhibitory effect on Ku binding to Tf elements (Figure 6E). ------- COMMENT: f895d3b39398c5aa 30 qyP9Xk0tlaY8bgdr2nr2ClpyvPo Figure 6E). H3K56 acetylation antagonizes Tf clustering at centromeres. binding of Ku was reduced and enhanced in hst4Δ and rtt109Δ cells, respectively ------- COMMENT: f895d3b39398c5aa 31 qyP9Xk0tlaY8bgdr2nr2ClpyvPo Figure 6E). H3K56 acetylation antagonizes Tf clustering at centromeres. binding of Ku was reduced and enhanced in hst4Δ and rtt109Δ cells, respectively ------- COMMENT: f895d3b39398c5aa 32 KTrikLazvylsnF9mui5Rtc4XvPs We observed that Ku localization was diffuse after DNA damage, but this diffusion was inhibited by rtt109Δ (Figure 7D) ------- COMMENT: f895d3b39398c5aa 33 zCLkjbf1TyQAiYgZjq4fLvGHjNo Figures 6A, 6B) (comment: see above) ------- COMMENT: f8a1d3758cd90a28 1 iaXvMxlFFexmbJjfwgTKvw+wlWs (Fig. 1A). ------- COMMENT: f8a1d3758cd90a28 2 H2GwFWjxm9bKfOc4dFU3PlLSq0s (Fig. 1B). (comment: decreasing slows after 6 hours) ------- COMMENT: f8a1d3758cd90a28 3 m7pXCy51H3a3d4CbzUeE0bwQXng (Fig. 1C). ------- COMMENT: f8a1d3758cd90a28 4 m7pXCy51H3a3d4CbzUeE0bwQXng (Fig. 1C). ------- COMMENT: f8a1d3758cd90a28 5 tUW+2uF9ggOoiH7TVUEJLI86iKE (Fig. 3). ------- COMMENT: f8a1d3758cd90a28 6 tUW+2uF9ggOoiH7TVUEJLI86iKE (Fig. 3). ------- COMMENT: f8b9e68a8b938c8d 1 ULYvxVwWDPUjqgW26Dru4yxwGXY Fig. 3A, 3B ------- COMMENT: f8b9e68a8b938c8d 2 80bY1KYgxMK4hiheZZSxxJIyFiI Fig. 3E, 3F ------- COMMENT: f8b9e68a8b938c8d 3 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: f8b9e68a8b938c8d 4 56Yt8xAl+Y3H2cN6GZ8HDGrXKaU Fig. 4D, 4E ------- COMMENT: f8b9e68a8b938c8d 5 7aiK1+OjgGLivIQBmNiGUezxkF0 Fig. 6G, S7F, S7G, (comment: TUBE pull-down) ------- COMMENT: f8b9e68a8b938c8d 6 3L1oMah6s2C6QhVwXNu32MZKHfA Fig. 7D, 7E (comment: tetrad dissection) ------- COMMENT: f8b9e68a8b938c8d 8 awAVyRdHNBBg7MhDE++9SbE3c4U (comment: tetrad disection) Fig. 7D, 7E ------- COMMENT: f8b9e68a8b938c8d 9 QpQBXo8YXyn/ZTf/iXjUEqyjn84 (comment: tetrad dioscection) Fig. 7C, 7E ------- COMMENT: f8b9e68a8b938c8d 10 +Ek/y8FI1jr63JOLTj8F9c2kaEQ Fig. S2B ------- COMMENT: f8b9e68a8b938c8d 11 eetFBEUP+KamIxws+25EP+YPZTg Fig. S3A, S3B (comment: control for increased proteasome in nucleus) ------- COMMENT: f8b9e68a8b938c8d 12 pN3jpQ0ET0miulof2/5kRQZ1tkg Fig. S3A, S3C ------- COMMENT: f8b9e68a8b938c8d 13 o1gJQzXM9pAsWA4jZKNaJd54aPM Fig. S3D ------- COMMENT: f8b9e68a8b938c8d 14 Get9myoSzi8gz/slqnlkyGH2jwg Fig. S3G, S3H ------- COMMENT: f8b9e68a8b938c8d 15 1/g8RIqX7Zz8pBBsLaFmQtWR+60 Fig. S3I, S3J ------- COMMENT: f8b9e68a8b938c8d 16 AOWyoCPqQ0oDc26UV8BhNLnpUx4 Fig. S6D ------- COMMENT: f8b9e68a8b938c8d 17 mlGI9egkE/G9tcsioeQiQHp2PH0 Fig. S6L, S6M ------- COMMENT: f8b9e68a8b938c8d 18 QJS/dyIUnM5Y6BKsztj8lmuMnjs Fig. 4G, 4H ------- COMMENT: f8b9e68a8b938c8d 21 1jjB9pQxqoWOHNo6HCBT1d22vA4 Fig. 1J ------- COMMENT: f8b9e68a8b938c8d 22 yoWZP4rekW9Yi+qHoVqubx3YiOk Fig. 1I ------- COMMENT: f8b9e68a8b938c8d 23 g2QP2oj9s42e+kqxQiRnBaPnvzM Figure 2A Plo1 to SPBs persisted for more than 20 min ------- COMMENT: f8b9e68a8b938c8d 24 TJDtKyn45Ce4KcF/h9Dc2EfMaBw In contrast, there was only a very slight delay in sister chromatid separation (Figures 2A and 2B). ------- COMMENT: f8b9e68a8b938c8d 25 u05cF5Es+2U24LxZhKxbMQ/5sEo chromosomes failed to split, but Plo1 was removed from SPBs with timing similar to that in wild-type cells (Figures 2A and 2C). ------- COMMENT: f8b9e68a8b938c8d 26 u05cF5Es+2U24LxZhKxbMQ/5sEo chromosomes failed to split, but Plo1 was removed from SPBs with timing similar to that in wild-type cells (Figures 2A and 2C). ------- COMMENT: f8b9e68a8b938c8d 28 cNt0cWHqJwAMWtrWOg0RHq0sALw (Figures 3B, 3D). sister chromatid separation (which depends on securin degradation, not on cyclin B degradation) was delayed as well ------- COMMENT: f8b9e68a8b938c8d 29 MMIA5XKl5NRSFfZ8SH9te5uxILw (Figure S2F export of CDK1 from the nucleus, which depends on cyclin B degradation ,, was delayed ------- COMMENT: f8fd03e7d6da971d 1 CB51aCJc1RszTIOR7eTw2uj21zA Table 2 the wis1 gene on a multi copy plasmid pwis1 can suppress the lethal phenotype of wee1-50 cdc25-22 win1-1 ------- COMMENT: f8fd03e7d6da971d 2 DpJFSlQ0EoLMCTFeCWv0xz2X3XI the wis2 gene on a multi copy plasmid pwis2 can suppress the lethal phenotype of wee1-50 cdc25-22 win1-1 ------- COMMENT: f8fd03e7d6da971d 3 HSrLtg2yJZJb8RzddR3JPCXMfC4 the spo12 gene on a multi copy plasmid pwis3 can suppress the lethal phenotype of wee1-50 cdc25-22 win1-1 ------- COMMENT: f8fd03e7d6da971d 4 mja3b5FweRXiFEcXkIem1kkKpkM the wis4 gene on a multi copy plasmid pwis4 can suppress the lethal phenotype of wee1-50 cdc25-22 win1-1 ------- COMMENT: f8fd03e7d6da971d 5 AwJZTBmJxl+Mv4XfmPkAtC39QTQ multicopy pwis1 does not suppress cdc25-22 ts phenotype showing that wis1 does not act by by directly reversing cdc25-22 loss of function ------- COMMENT: f8fd03e7d6da971d 6 DeewI+CWCXjt2r7sHEkXIXzV2/0 multicopy pwis2 does not suppress cdc25-22 ts phenotype showing that wis2 does not act by by directly reversing cdc25-22 loss of function ------- COMMENT: f8fd03e7d6da971d 7 SbfjB9dJskpeyckNFL0glzdDiew multicopy pwis3 does not suppress cdc25-22 ts phenotype showing that spo12 does not act by by directly reversing cdc25-22 loss of function ------- COMMENT: f8fd03e7d6da971d 8 FRJRuRbT48PbHHk6oMzckcKAAFM multicopy pwis4 does not suppress cdc25-22 ts phenotype showing that wis4 does not act by by directly reversing cdc25-22 loss of function ------- COMMENT: f8fd03e7d6da971d 9 uYu5Smuc4E9qxGGewNIzAUv4JAY multicopy pwis1 does not suppress cdc2-33 ts phenotype ------- COMMENT: f8fd03e7d6da971d 10 fE1yssT2AOMt3H8hyRZ1DKqPAn0 multicopy pwis1 does not suppress cdc13-117 ts phenotype ------- COMMENT: f8fd03e7d6da971d 11 er4lNPOto7ujbVrh6jwySnh/rtE multicopy pwis1 does not suppress cdc2-1w phenotype ------- COMMENT: f8fd03e7d6da971d 12 imuwSyKXs71GP8JPC8M7O25WLk0 multicopy pwis1 does not suppress cdc2-3w phenotype ------- COMMENT: f8fd03e7d6da971d 13 OTHATvXqs7kahyf9/OGbD0m/ag0 multicopy pwis1 does not suppress wee1-50 ts phenotype ------- COMMENT: f8fd03e7d6da971d 14 A+P5kHG4MvBwKmtEhObSmZ8O428 multicopy pwis1 does not suppress cdr1-34 phenotype ------- COMMENT: f8fd03e7d6da971d 15 eshYpjwCCEPBWJtf0yX2ncsEaqI multicopy pwis1 does not suppress cdr2-69 phenotype ------- COMMENT: f8fd03e7d6da971d 16 1SsmA73fJ2UHshLzQ1ol0GccQeg multicopy pwis2 does not suppress cdc2-33 ts phenotype ------- COMMENT: f8fd03e7d6da971d 17 uVgEYN1kWKCQYMbQ14qTovY+CVQ multicopy pwis2 does not suppress cdc13-117 ts phenotype ------- COMMENT: f8fd03e7d6da971d 18 LrKyVB52FfOR0XfpuJw+DRUo+Es multicopy pwis2 does not suppress cdc2-1w phenotype ------- COMMENT: f8fd03e7d6da971d 19 M6txUVcFaoEapU3pZ8jjaGp6oeg multicopy pwis2 does not suppress cdc2-3w phenotype ------- COMMENT: f8fd03e7d6da971d 20 LMj1WIC/rOXxn/MHBIE5yIlTPSs multicopy pwis2 does not suppress wee1-50 ts phenotype ------- COMMENT: f8fd03e7d6da971d 21 BgDAcU3gYp6xxCdr0hbE3NJ6fDs multicopy pwis2 does not suppress cdr1-34 phenotype ------- COMMENT: f8fd03e7d6da971d 22 anA1pDkj38s8zGN0nGUUfHEL5q4 multicopy pwis2 does not suppress cdr2-69 phenotype ------- COMMENT: f8fd03e7d6da971d 23 5e2cJ3nLUNuty6ExPdXQb2NJy64 multicopy pwis3 does not suppress cdc2-33 ts phenotype ------- COMMENT: f8fd03e7d6da971d 24 UsG2e3/892CAF2AyiU2kYiadVuM multicopy pwis3 does not suppress cdc13-117 ts phenotype ------- COMMENT: f8fd03e7d6da971d 25 ZBgfckVMJ2vfscMv3+ERipYunBI multicopy pwis3 does not suppress cdc2-1w phenotype ------- COMMENT: f8fd03e7d6da971d 26 pqgaup5PpC9EL0o63nAP4CwHHsk multicopy pwis3 does not suppress cdc2-3w phenotype ------- COMMENT: f8fd03e7d6da971d 27 NzxKOXLO/eVAxlhMhqps64VuW5I multicopy pwis3 does not suppress wee1-50 ts phenotype ------- COMMENT: f8fd03e7d6da971d 28 8lN3GlG3L7jffpepEAu8HpRkIXU multicopy pwis3 does not suppress cdr1-34 phenotype ------- COMMENT: f8fd03e7d6da971d 29 FYbUTou6LcpCgvyc+Ov2sg9Ffq4 multicopy pwis3 does not suppress cdr2-69 phenotype ------- COMMENT: f8fd03e7d6da971d 30 JjkWqsRC1Ls/tTqZAEyX+FKMMAM multicopy pwis4 does not suppress cdc2-33 ts phenotype ------- COMMENT: f8fd03e7d6da971d 31 adDCoP0fBxxpb73/Adn+ji3dvvY multicopy pwis4 does not suppress cdc13-117 ts phenotype ------- COMMENT: f8fd03e7d6da971d 32 hILNRbPyaBxjyqb+4+meyJN3a5I multicopy pwis4 does not suppress cdc2-1w phenotype ------- COMMENT: f8fd03e7d6da971d 33 2mosRXVpI/Pr6FNSyMFslfY+S5g multicopy pwis4 does not suppress cdc2-3w phenotype ------- COMMENT: f8fd03e7d6da971d 34 ZRMV/+qObF2qUwUJOtL++je7WEE multicopy pwis4 does not suppress wee1-50 ts phenotype ------- COMMENT: f8fd03e7d6da971d 35 bRfiIk87cj7FNx6FIymHOiYyM7I multicopy pwis4 does not suppress cdr1-34 phenotype ------- COMMENT: f8fd03e7d6da971d 36 8KSFzz/Rt49Kgdn1V9OwlMlMwME multicopy pwis4 does not suppress cdr2-69 phenotype ------- COMMENT: f8fd03e7d6da971d 37 mLMO6kHvldhXVRPKSH5K2C9z6aY see Table 2 ------- COMMENT: f8fd03e7d6da971d 38 mLMO6kHvldhXVRPKSH5K2C9z6aY see Table 2 ------- COMMENT: f8fd03e7d6da971d 39 mLMO6kHvldhXVRPKSH5K2C9z6aY see Table 2 ------- COMMENT: f8fd03e7d6da971d 40 2kZqJYNA+VCIebc4ztUD2PMwajk See Table 2 multi copy pwis4 does not suppress cdc25-22 wee1-50 mcs4-13 ------- COMMENT: f8fd03e7d6da971d 41 xWZc3ZI2AdzRCz3Q5W0mN6ThtWs see Table 2 multi copy pwis4 does not suppress cdc25-22 wee1-50 mcs4-13 ------- COMMENT: f8fd03e7d6da971d 42 DS5XdLvw8OvIfUJK/gCvxeigyIQ Table 2 pwis1 does not suppress wee1-50 cdc25-22 mcs6-13 ------- COMMENT: f8fd03e7d6da971d 43 DS5XdLvw8OvIfUJK/gCvxeigyIQ Table 2 pwis1 does not suppress wee1-50 cdc25-22 mcs6-13 ------- COMMENT: f8fd03e7d6da971d 44 mLMO6kHvldhXVRPKSH5K2C9z6aY see Table 2 ------- COMMENT: f8fd03e7d6da971d 45 mLMO6kHvldhXVRPKSH5K2C9z6aY see Table 2 ------- COMMENT: f8fd03e7d6da971d 46 laosEIT0OcmyofiAfsc7LoSJwjE Table 2 pwis4 does not suppress wee1-50 cdc25-22 mcs6-13 ------- COMMENT: f8fd03e7d6da971d 47 laosEIT0OcmyofiAfsc7LoSJwjE Table 2 pwis4 does not suppress wee1-50 cdc25-22 mcs6-13 ------- COMMENT: f8fd03e7d6da971d 48 mLMO6kHvldhXVRPKSH5K2C9z6aY see Table 2 ------- COMMENT: f8fd03e7d6da971d 49 Eln+4Mq8CiNsIoZfHo5dRSSIwtQ the wis1 gene on a multi copy plasmid pwis1 can suppress the lethal phenotype of wee1-50 cdc25-22 win1-1 ------- COMMENT: f8fd03e7d6da971d 50 DpJFSlQ0EoLMCTFeCWv0xz2X3XI the wis2 gene on a multi copy plasmid pwis2 can suppress the lethal phenotype of wee1-50 cdc25-22 win1-1 ------- COMMENT: f8fd03e7d6da971d 51 HSrLtg2yJZJb8RzddR3JPCXMfC4 the spo12 gene on a multi copy plasmid pwis3 can suppress the lethal phenotype of wee1-50 cdc25-22 win1-1 ------- COMMENT: f8fd03e7d6da971d 52 mja3b5FweRXiFEcXkIem1kkKpkM the wis4 gene on a multi copy plasmid pwis4 can suppress the lethal phenotype of wee1-50 cdc25-22 win1-1 ------- COMMENT: f8fd03e7d6da971d 53 AwJZTBmJxl+Mv4XfmPkAtC39QTQ multicopy pwis1 does not suppress cdc25-22 ts phenotype showing that wis1 does not act by by directly reversing cdc25-22 loss of function ------- COMMENT: f8fd03e7d6da971d 54 SbfjB9dJskpeyckNFL0glzdDiew multicopy pwis3 does not suppress cdc25-22 ts phenotype showing that spo12 does not act by by directly reversing cdc25-22 loss of function ------- COMMENT: f8fd03e7d6da971d 55 uYu5Smuc4E9qxGGewNIzAUv4JAY multicopy pwis1 does not suppress cdc2-33 ts phenotype ------- COMMENT: f8fd03e7d6da971d 56 fE1yssT2AOMt3H8hyRZ1DKqPAn0 multicopy pwis1 does not suppress cdc13-117 ts phenotype ------- COMMENT: f8fd03e7d6da971d 57 1SsmA73fJ2UHshLzQ1ol0GccQeg multicopy pwis2 does not suppress cdc2-33 ts phenotype ------- COMMENT: f8fd03e7d6da971d 58 uVgEYN1kWKCQYMbQ14qTovY+CVQ multicopy pwis2 does not suppress cdc13-117 ts phenotype ------- COMMENT: f8fd03e7d6da971d 59 5e2cJ3nLUNuty6ExPdXQb2NJy64 multicopy pwis3 does not suppress cdc2-33 ts phenotype ------- COMMENT: f8fd03e7d6da971d 60 UsG2e3/892CAF2AyiU2kYiadVuM multicopy pwis3 does not suppress cdc13-117 ts phenotype ------- COMMENT: f8fd03e7d6da971d 61 JjkWqsRC1Ls/tTqZAEyX+FKMMAM multicopy pwis4 does not suppress cdc2-33 ts phenotype ------- COMMENT: f8fd03e7d6da971d 62 adDCoP0fBxxpb73/Adn+ji3dvvY multicopy pwis4 does not suppress cdc13-117 ts phenotype ------- COMMENT: f8fd03e7d6da971d 63 DeewI+CWCXjt2r7sHEkXIXzV2/0 multicopy pwis2 does not suppress cdc25-22 ts phenotype showing that wis2 does not act by by directly reversing cdc25-22 loss of function ------- COMMENT: f8fd03e7d6da971d 64 FRJRuRbT48PbHHk6oMzckcKAAFM multicopy pwis4 does not suppress cdc25-22 ts phenotype showing that wis4 does not act by by directly reversing cdc25-22 loss of function ------- COMMENT: f8fd03e7d6da971d 65 cITrjCnvOx2297wIqJYWSVUdvQk Table 3 cells are 30-50% longer than wild type ------- COMMENT: f8fd03e7d6da971d 66 9POs9GAhfNg389NGlIyIqQcdyYw Table 3 pwis4 surpresses the elongated cell phenotype of win1-1 ------- COMMENT: f8fd03e7d6da971d 67 1+h9OGZwOlaOfHwgYE3uupJDJNo data not shown ------- COMMENT: f8fd03e7d6da971d 68 mLMO6kHvldhXVRPKSH5K2C9z6aY see Table 2 ------- COMMENT: f8fd03e7d6da971d 69 mLMO6kHvldhXVRPKSH5K2C9z6aY see Table 2 ------- COMMENT: f8fd03e7d6da971d 70 mLMO6kHvldhXVRPKSH5K2C9z6aY see Table 2 ------- COMMENT: f8fd03e7d6da971d 71 mLMO6kHvldhXVRPKSH5K2C9z6aY see Table 2 ------- COMMENT: f90ddb2fa9bdc30c 1 +ghJ/6M5hZjM0jc4hSlaWVGwpkw (comment: CHECK lethal >34°C) (Fig. 1c). ------- COMMENT: f90ddb2fa9bdc30c 2 rE7+CNXbU1GSFqVoO4CksC5VaNc reduction in Pht1Ac (Fig. 1d), indicating that Pht1 acetylation is Mst1-dependent. ------- COMMENT: f90ddb2fa9bdc30c 3 WxKQx0g98DnB0hIONIG7k22vIj0 Cell fractionation showed that Pht1Ac is chromatin-associated, though acetylation is not required for entry to this cellular compartment (Figs. 1e–f) ------- COMMENT: f90ddb2fa9bdc30c 4 vR4QHsxCJEwQST2EeYj9K2Gc4Ts figure2b ------- COMMENT: f90ddb2fa9bdc30c 5 vR4QHsxCJEwQST2EeYj9K2Gc4Ts figure2b ------- COMMENT: f90ddb2fa9bdc30c 6 vR4QHsxCJEwQST2EeYj9K2Gc4Ts figure2b ------- COMMENT: f90ddb2fa9bdc30c 7 vR4QHsxCJEwQST2EeYj9K2Gc4Ts figure2b ------- COMMENT: f90ddb2fa9bdc30c 8 vR4QHsxCJEwQST2EeYj9K2Gc4Ts figure2b ------- COMMENT: f90ddb2fa9bdc30c 9 vR4QHsxCJEwQST2EeYj9K2Gc4Ts figure2b ------- COMMENT: f90ddb2fa9bdc30c 10 vR4QHsxCJEwQST2EeYj9K2Gc4Ts figure2b ------- COMMENT: f90ddb2fa9bdc30c 11 vR4QHsxCJEwQST2EeYj9K2Gc4Ts figure2b ------- COMMENT: f90ddb2fa9bdc30c 13 vR4QHsxCJEwQST2EeYj9K2Gc4Ts figure2b ------- COMMENT: f90ddb2fa9bdc30c 14 vR4QHsxCJEwQST2EeYj9K2Gc4Ts figure2b ------- COMMENT: f90ddb2fa9bdc30c 15 vR4QHsxCJEwQST2EeYj9K2Gc4Ts figure2b ------- COMMENT: f90ddb2fa9bdc30c 16 vR4QHsxCJEwQST2EeYj9K2Gc4Ts figure2b ------- COMMENT: f90ddb2fa9bdc30c 17 vR4QHsxCJEwQST2EeYj9K2Gc4Ts figure2b ------- COMMENT: f90ddb2fa9bdc30c 18 vR4QHsxCJEwQST2EeYj9K2Gc4Ts figure2b ------- COMMENT: f90ddb2fa9bdc30c 19 vR4QHsxCJEwQST2EeYj9K2Gc4Ts figure2b ------- COMMENT: f90ddb2fa9bdc30c 20 2bbdPWyPbQf7GRLQUdKTcP4ojG8 p SWR-C required for the efficient acetylation of the histone (Fig. 2b), most likely because of inefficient assembly of the variant into chromatin in each background (Fig. 2c). Thus a pathway first identified in Sc also operates in Sp: SWR-C inserts Pht1 into chromatin, where it is acetylated by Mst1. ------- COMMENT: f90ddb2fa9bdc30c 21 4M7v/1/Zh1Xvv4Y5eJq6EhXQ/FU Knockout or depletion of H2A.Z in Sc 15 or mammalian cells 5 leads to increased rates of chromosome loss. This phenotype was also observed if any component of the Sp Pht1Ac pathway is disrupted, including mutants in swr1 (and msc1), pht1 (pht1Δ, −4KR or −4KQ), or mst1 (Supplementary Table 2 and 16,25,26). ------- COMMENT: f90ddb2fa9bdc30c 22 4M7v/1/Zh1Xvv4Y5eJq6EhXQ/FU Knockout or depletion of H2A.Z in Sc 15 or mammalian cells 5 leads to increased rates of chromosome loss. This phenotype was also observed if any component of the Sp Pht1Ac pathway is disrupted, including mutants in swr1 (and msc1), pht1 (pht1Δ, −4KR or −4KQ), or mst1 (Supplementary Table 2 and 16,25,26). ------- COMMENT: f90ddb2fa9bdc30c 23 4M7v/1/Zh1Xvv4Y5eJq6EhXQ/FU Knockout or depletion of H2A.Z in Sc 15 or mammalian cells 5 leads to increased rates of chromosome loss. This phenotype was also observed if any component of the Sp Pht1Ac pathway is disrupted, including mutants in swr1 (and msc1), pht1 (pht1Δ, −4KR or −4KQ), or mst1 (Supplementary Table 2 and 16,25,26). ------- COMMENT: f90ddb2fa9bdc30c 24 4M7v/1/Zh1Xvv4Y5eJq6EhXQ/FU Knockout or depletion of H2A.Z in Sc 15 or mammalian cells 5 leads to increased rates of chromosome loss. This phenotype was also observed if any component of the Sp Pht1Ac pathway is disrupted, including mutants in swr1 (and msc1), pht1 (pht1Δ, −4KR or −4KQ), or mst1 (Supplementary Table 2 and 16,25,26). ------- COMMENT: f90ddb2fa9bdc30c 25 4M7v/1/Zh1Xvv4Y5eJq6EhXQ/FU Knockout or depletion of H2A.Z in Sc 15 or mammalian cells 5 leads to increased rates of chromosome loss. This phenotype was also observed if any component of the Sp Pht1Ac pathway is disrupted, including mutants in swr1 (and msc1), pht1 (pht1Δ, −4KR or −4KQ), or mst1 (Supplementary Table 2 and 16,25,26). ------- COMMENT: f90ddb2fa9bdc30c 26 4M7v/1/Zh1Xvv4Y5eJq6EhXQ/FU Knockout or depletion of H2A.Z in Sc 15 or mammalian cells 5 leads to increased rates of chromosome loss. This phenotype was also observed if any component of the Sp Pht1Ac pathway is disrupted, including mutants in swr1 (and msc1), pht1 (pht1Δ, −4KR or −4KQ), or mst1 (Supplementary Table 2 and 16,25,26). ------- COMMENT: f90ddb2fa9bdc30c 27 4M7v/1/Zh1Xvv4Y5eJq6EhXQ/FU Knockout or depletion of H2A.Z in Sc 15 or mammalian cells 5 leads to increased rates of chromosome loss. This phenotype was also observed if any component of the Sp Pht1Ac pathway is disrupted, including mutants in swr1 (and msc1), pht1 (pht1Δ, −4KR or −4KQ), or mst1 (Supplementary Table 2 and 16,25,26). ------- COMMENT: f90ddb2fa9bdc30c 28 PLPPvcD54a+J26JwMy+NSJfK0hM (iii) entanglement leading to breakage, where broken pieces of chromatin with no kinetochore lag on the spindle (Fig. 4b). ------- COMMENT: f90ddb2fa9bdc30c 29 PLPPvcD54a+J26JwMy+NSJfK0hM (iii) entanglement leading to breakage, where broken pieces of chromatin with no kinetochore lag on the spindle (Fig. 4b). ------- COMMENT: f90ddb2fa9bdc30c 30 UQYL+F4xq0xmN1FxZsx1LEEiFB0 This partial rescue was specific, as pht1Δ was synthetic with rad21-K1, a mutant in the condensin- related complex cohesin, which holds sister-chromatids together prior to anaphase onset. ------- COMMENT: f93022f938a65fbf 1 RZYWbnOR8vUftLg+MOUT87I5V0U In cells lacking Sgo1 (sgo1Δ), which protects centromeric cohesin during anaphase, no separated kinetochore signals were observed (Fig. 3A,C), although sister chromatids frequently underwent equational segregation in the absence of chiasmata (Fig. S2B). ------- COMMENT: f93022f938a65fbf 3 N0BnB994mpRKHy0hKoIunq9hyqE Non-separated signals were found to be significantly or nearly significantly wider than in wild-type cells (Fig. 3E,F;) ------- COMMENT: f93022f938a65fbf 5 IxHrwIIuZhPic1J14xQgnE50YmY In mrc1Δ cells, sister kinetochores and centromere cores separated at a low, but significant, level (Fig. 3A–D; Figs S2C and S3A,B), and non-separated signals were significantly wider in shape (Fig. 3E,F; Fig. S3C,D). ------- COMMENT: f93022f938a65fbf 9 WmCBlxRzpZgYmm0OlOSG2T1xs6g Additionally, the DNA replication checkpoint function of Mrc1 is not required for sister kinetochore association, because deletion of cds1, which encodes an effector kinase functioning downstream of Mrc1 in the DNA replication checkpoint pathway (Alcasabas et al., 2001; Murakami and Okayama, 1995; Tanaka and Russell, 2001), did not affect the kinetochore association state or sister chromatid segregation (Fig. 3C,E; Fig. S2C). ------- COMMENT: f938aca48cd3f966 3 OZ7bZ8e5d9fCKEcqEeuS5zNu6B0 Figures 2A, S1A; Movie S1 ------- COMMENT: f938aca48cd3f966 4 7Qgl6Vc4Wg8+hYCM9rRxuCeWUnI Figure S1B ------- COMMENT: f938aca48cd3f966 5 ljsiwRtyuLGxvuJ8YocDHS//SiA unlike WT cell elongation continued after actin depolymerization, (Figures 2A and 2B; Movie S1) conclusions. 1. the SAPK pathway is required for CRIB dispersal after LatA treatment. 2 the actin cytoskeleton per se is not required for stability of the Cdc42 polarity module at cell tips. 3. cell elongation can occur in the complete absence of the actin cytoskeleton. ------- COMMENT: f938aca48cd3f966 6 ljsiwRtyuLGxvuJ8YocDHS//SiA unlike WT cell elongation continued after actin depolymerization, (Figures 2A and 2B; Movie S1) conclusions. 1. the SAPK pathway is required for CRIB dispersal after LatA treatment. 2 the actin cytoskeleton per se is not required for stability of the Cdc42 polarity module at cell tips. 3. cell elongation can occur in the complete absence of the actin cytoskeleton. ------- COMMENT: f938aca48cd3f966 8 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: f938aca48cd3f966 9 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: f938aca48cd3f966 10 Mc1Dl/8ePjG+W3BQIevC1vaEvqw Figure 2D ------- COMMENT: f938aca48cd3f966 12 GC++j9yrmGrN29cDVzZsnaOCe40 Figures S1C, S1D ------- COMMENT: f938aca48cd3f966 13 GC++j9yrmGrN29cDVzZsnaOCe40 Figures S1C, S1D ------- COMMENT: f938aca48cd3f966 15 WXP0U5MC7CFt29Wl+3GG8EhgFBQ (comment: arrested) ------- COMMENT: f938aca48cd3f966 16 yp1/U4E7fyf1Tk/RwARStQ6z5KM Movie S4. Sty1 activity is critical for maintaining a non-polarized Cdc42 module in N-starved quiescent cells. ------- COMMENT: f938aca48cd3f966 17 QO8KsW+0cG803GW+Pt1xxYlzJ1c (comment: CHECK during nitrogen starvation). Although many mutants in the SAPK pathway have defects in mating and meiosis, this result may help to explain why sty1D and wis1D mutants in particular continue to elongate upon N starvation, unlike other mutants in the pathway ------- COMMENT: f938aca48cd3f966 19 yp1/U4E7fyf1Tk/RwARStQ6z5KM Movie S4. Sty1 activity is critical for maintaining a non-polarized Cdc42 module in N-starved quiescent cells. ------- COMMENT: f9396b475a851162 1 QNwbQI/XINqjpn1BoVpWF0drqT4 (comment: this isn't reall demonstrated here but it can ne imputed form this experiment and prior knowledge) ------- COMMENT: f965fe048f417771 13 nnpBlJVmHEspOKmJ6hvTYGDzB5w Supplementary Figure S1A-E ------- COMMENT: f965fe048f417771 14 LGhNZD2zbn/trDdICgwuygS1a2Q Figure 2C, Supplementary Figure S3A and C ------- COMMENT: f965fe048f417771 15 LGhNZD2zbn/trDdICgwuygS1a2Q Figure 2C, Supplementary Figure S3A and C ------- COMMENT: f965fe048f417771 16 LGhNZD2zbn/trDdICgwuygS1a2Q Figure 2C, Supplementary Figure S3A and C ------- COMMENT: f965fe048f417771 17 sJ88YmTl/m0udCFesBxfjrB58Zg (Fig- ure 2D, Supplementary Figure S3B and C ------- COMMENT: f965fe048f417771 18 sJ88YmTl/m0udCFesBxfjrB58Zg (Fig- ure 2D, Supplementary Figure S3B and C ------- COMMENT: f965fe048f417771 19 jy3YXh0Rjhk2QbjNKcboeVUFHvk Figure 2C, D, Supple- mentary Figure S3A and C ------- COMMENT: f965fe048f417771 20 jy3YXh0Rjhk2QbjNKcboeVUFHvk Figure 2C, D, Supple- mentary Figure S3A and C ------- COMMENT: f965fe048f417771 21 VnyEUbDJwyHaRXjZn1e9HwzwPVQ Supple- mentary Figure S4A) ------- COMMENT: f965fe048f417771 22 VnyEUbDJwyHaRXjZn1e9HwzwPVQ Supple- mentary Figure S4A) ------- COMMENT: f965fe048f417771 23 VnyEUbDJwyHaRXjZn1e9HwzwPVQ Supple- mentary Figure S4A) ------- COMMENT: f965fe048f417771 24 AcJ24C3K7nRzJWiGw4JCWS/DGRQ Figure 2E and Supple- mentary Figure S4B ------- COMMENT: f965fe048f417771 25 VnyEUbDJwyHaRXjZn1e9HwzwPVQ Supple- mentary Figure S4A) ------- COMMENT: f965fe048f417771 26 +3xktnLqALBU/Nss3ezLwxsaA3Y Supple- mentary Figure S5B ------- COMMENT: f965fe048f417771 27 +3xktnLqALBU/Nss3ezLwxsaA3Y Supple- mentary Figure S5B ------- COMMENT: f965fe048f417771 28 +3xktnLqALBU/Nss3ezLwxsaA3Y Supple- mentary Figure S5B ------- COMMENT: f965fe048f417771 29 +3xktnLqALBU/Nss3ezLwxsaA3Y Supple- mentary Figure S5B ------- COMMENT: f965fe048f417771 30 +3xktnLqALBU/Nss3ezLwxsaA3Y Supple- mentary Figure S5B ------- COMMENT: f965fe048f417771 31 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: f965fe048f417771 32 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: f965fe048f417771 33 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: f965fe048f417771 34 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: f965fe048f417771 35 /TBoxxfFdMg5CDIkCsPx1JqgtsU Figure 3B and Supplementary Figure S6 ------- COMMENT: f965fe048f417771 36 /TBoxxfFdMg5CDIkCsPx1JqgtsU Figure 3B and Supplementary Figure S6 ------- COMMENT: f965fe048f417771 37 /TBoxxfFdMg5CDIkCsPx1JqgtsU Figure 3B and Supplementary Figure S6 ------- COMMENT: f965fe048f417771 38 /TBoxxfFdMg5CDIkCsPx1JqgtsU Figure 3B and Supplementary Figure S6 ------- COMMENT: f965fe048f417771 39 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: f965fe048f417771 40 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: f965fe048f417771 41 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: f965fe048f417771 42 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: f965fe048f417771 45 IN8Vcaf91ImKpslUS3xFbe5FmaQ Supplementary Figure S7A-D) ------- COMMENT: f965fe048f417771 46 MjpnWi8onv5BpGsaRUHtdcr/BWc (comment: competatively with sad1) ------- COMMENT: f965fe048f417771 47 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: f965fe048f417771 48 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: f965fe048f417771 49 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: f965fe048f417771 50 mFIEe7KtQcW96VF2bcO9tTTRhrA Supplementary Figure S7E ------- COMMENT: f965fe048f417771 51 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: f965fe048f417771 52 mFIEe7KtQcW96VF2bcO9tTTRhrA Supplementary Figure S7E ------- COMMENT: f965fe048f417771 53 mFIEe7KtQcW96VF2bcO9tTTRhrA Supplementary Figure S7E ------- COMMENT: f965fe048f417771 54 q0kKUEbwBc/ao+0FQ7AQ2MkP78U Figure 4F and Supplementary Figure S7F ------- COMMENT: f965fe048f417771 55 uOP4aZj2kJ1lhV4M8TlbZH0M39s (comment: competatively with lem2) ------- COMMENT: f965fe048f417771 56 ulmKU+AWf/Ui1CCq3SfikS2WvEg Figure 6B and Supplementary Fig- ure S8B ------- COMMENT: f965fe048f417771 57 ulmKU+AWf/Ui1CCq3SfikS2WvEg Figure 6B and Supplementary Fig- ure S8B ------- COMMENT: f965fe048f417771 58 ulmKU+AWf/Ui1CCq3SfikS2WvEg Figure 6B and Supplementary Fig- ure S8B ------- COMMENT: f965fe048f417771 59 uUQo7EsSH9+ld7Gno0aes5374Ac Figure 6B and Supplementary Figure S8C) ------- COMMENT: f965fe048f417771 60 CKoJeR+s8gdKYP1ePOl4I0Em9aI Figure 6B and Supple- mentary Figure S8B ------- COMMENT: f965fe048f417771 61 CKoJeR+s8gdKYP1ePOl4I0Em9aI Figure 6B and Supple- mentary Figure S8B ------- COMMENT: f965fe048f417771 62 YYvmBmaXAL5BjpX8ar5DkLfarnI Figure 6B and Supplementary Figure S8C ------- COMMENT: f965fe048f417771 63 oMKdulln9CNfV8eu3c6Pr8a5Wfo (comment: suggested by Junko) ------- COMMENT: f96ed996276b8e88 5 opg91ZvWyHl4M1rSmzRhbnoIe0s (comment: binds H3K9me) ------- COMMENT: f96ee693f519ef9d 1 XTBtOsgRKCQTJUeMIs8hV9mWkNM Fig2A, 2B, Fig 4C, (comment: pREP5cdc2YFP integrant grown in YE+supplements (i.e. promoter OFF)) Proportion of total of Cdc2YFP in nucleus and cytoplasm varies across cell cycle. Lowest in nucleus during late mitosis (~ 10% of total), highest in nucleus in late G2 (~30-40%) of total ------- COMMENT: f96ee693f519ef9d 2 pQTd2br+1IFSEkETTj2dy2w+EEQ Fig2A (comment: pREP5cdc13-YFP integrant grown in YE+supplements (i.e. promoter OFF)) ------- COMMENT: f96ee693f519ef9d 4 +1y5nx+qQuATBYEucvo2905RZ58 Fig 2A b-c, Fig5D (comment: pREP5cdc13YFP integrant grown in YE+supplements (i.e. promoter OFF).) ------- COMMENT: f96ee693f519ef9d 5 jmVpAvMiBVyx+s5YH1twcWO651c Fig 2A b-c, Fig5 (comment: pREP5cdc2YFP integrant grown in YE+supplements (i.e. promoter OFF).) ------- COMMENT: f96ee693f519ef9d 6 9HLwnQ5fVGJSo/xBf8Dey/nqIoM Fig2A, 2B (comment: pREP5cdc2YFP integrant grown in YE+supplements (i.e. promoter OFF).) Proportion of total of Cdc2YFP in nucleus and cytoplasm varies across cell cycle. Lowest in nucleus during late mitosis (~ 10% of total), highest in nucleus in late G2 (~30-40%) of total ------- COMMENT: f96ee693f519ef9d 7 AouhcYG3lNI75BVDrZ8xKbtS24I Fig 2A, Fig 3, Fig5D (comment: pREP5cdc13YFP integrant grown in YE+supplements (i.e. promoter OFF).) ------- COMMENT: f96ee693f519ef9d 8 rWCS5Yxftygg78LZb0DIOJl9Vio Fig 2A, Fig3, Fig5D (comment: pREP5cdc2YFP integrant grown in YE+supplements (i.e. promoter OFF).) ------- COMMENT: f96ee693f519ef9d 9 oO3RsbjSQ8vO5BiFl2JEf/jvmTg Fig 2C, (comment: pREP5cdc13YFP integrant grown in YE+supplements (i.e. promoter OFF).) ------- COMMENT: f96ee693f519ef9d 10 ss0HUpOpBBdPy6WIDBpgLEkHa+Q Figure 4A ------- COMMENT: f96ee693f519ef9d 11 AoIE2/tebXNFZPwNdEs8pCgWJlE Figure 4A. (comment: Cdc13YFP expressed from integrated pREP45) ------- COMMENT: f96ee693f519ef9d 12 ofOh7R86RzAp012T4iZOHL6OAdQ Figure 4A. (comment: Cdc13YFP expressed from integrated pREP45.) ------- COMMENT: f96ee693f519ef9d 17 ItfuGyx3xdgz1QRhgvAnpWWoSNU Data not shown. (comment: Cdc2 does not go prematurely to the SPB in a cut12 mutant (this is the stf1-1 mutant)) ------- COMMENT: f96ee693f519ef9d 18 pwrLWBiiuIwi7xgnYWY5+o0bCrQ Figure 4B. (comment: Cdc13YFP expressed from integrated pREP45) ------- COMMENT: f96ee693f519ef9d 19 XAa0JugKjzty90TiroMq2GvqVKk Figure 4A. (comment: Cdc13YFP expressed from integrated pREP45.) Decreased nuclear import of cdc2YFP compared to cdc13delta cig1delta mutant ------- COMMENT: f96ee693f519ef9d 20 u1mx/x0SxHc6p/h/44S5p+bMNdA Fig 6 ------- COMMENT: f96ee693f519ef9d 21 OxvMLMhQr7G5tXj0isHQRuzZ+3M Fig 6 Cdc2YFP and non-degradable Cdc13YFP remain associated with spindle, SPB.Cdc13 degradation is abolished rather than delayed ------- COMMENT: f96ee693f519ef9d 22 ken7D5wy9fbPR94Bh7IKX4z0gJ8 Fig 6 Cdc13YFP and Cdc2YFP remain associated with spindle, SPB. Cdc13 is not recognised by defective APC ------- COMMENT: f96ee693f519ef9d 23 gz++2nRu1WWjfZcXlIuiuOUMfmU Fig 6 Cdc13YFP and Cdc2YFP remain associated with spindle, SPB. Cdc13 is not degraded by defective proteasome. Rpt1 is called Mts2 in this paper ------- COMMENT: f96ee693f519ef9d 24 hNbQp+mffGwXRT0sqc7LXtvc0Po Fig 7 (comment: do not actually say it is associated with SPB just SPB region, i.e. telomere-SPB- centromere bouquet cluster) ------- COMMENT: f96ee693f519ef9d 25 a2ABRFqZ8Kas2+zmrBbwTMfjtzc cdc2 is localised at the centromeres during horse tail movement. Fig 9 shows that cdc2YFP is associated with cen1GFP ------- COMMENT: f96ee693f519ef9d 26 steeyeTIf0tHuMWCSd7Sj4GAFSY Fig 8 ------- COMMENT: f96ee693f519ef9d 29 TBMsUvnlYcDpEmeGUAH2Kz1ylZ4 Fig 7 ------- COMMENT: f96ee693f519ef9d 30 TBMsUvnlYcDpEmeGUAH2Kz1ylZ4 Fig 7 ------- COMMENT: f96ee693f519ef9d 31 TBMsUvnlYcDpEmeGUAH2Kz1ylZ4 Fig 7 ------- COMMENT: f988eca9cbf13b9a 1 hZ1rIDr/3WlFZk/9RPhujwsRYQc Figure 4A; decreased frequency of deletions and gene conversions at direct repeat recombination reporter ------- COMMENT: f988eca9cbf13b9a 2 taHVVPvfApMZI06yi8skeWmT3T0 Figure 4C; increased frequency of deletions at direct repeat recombination reporter ------- COMMENT: f988eca9cbf13b9a 3 CeGb6RzsPHpYo5DBlDVjdgVnijg Figure 4C; decreased frequency of gene conversions at direct repeat recombination reporter ------- COMMENT: f988eca9cbf13b9a 4 IuPCDy2C5ecOeBnmoMjosnsZlec Figure 4B; decreased frequency of deletions at direct repeat recombination reporter ------- COMMENT: f988eca9cbf13b9a 5 voR3diUI5/DIf9Tb71TIs4Qd+To Figure 2; decreased frequency of gene conversions but unaltered frequency of deletions at direct repeat recombination reporter; deletions in a rad51∆ mutant depend on Rad52 ------- COMMENT: f9a2c4769a3f8ab9 1 goEO9S3UHQnaYolcF2Vye6cN6UI (comment: dependent on F-actin (assayed using Latrunculin A)) ------- COMMENT: f9b779cdcdfe781f 3 /BZjPAvUyZcRggNcLIb+lQAvvFI table2 ------- COMMENT: f9b779cdcdfe781f 4 /BZjPAvUyZcRggNcLIb+lQAvvFI table2 ------- COMMENT: f9b779cdcdfe781f 5 /BZjPAvUyZcRggNcLIb+lQAvvFI table2 ------- COMMENT: f9b779cdcdfe781f 6 /BZjPAvUyZcRggNcLIb+lQAvvFI table2 ------- COMMENT: f9b779cdcdfe781f 7 /BZjPAvUyZcRggNcLIb+lQAvvFI table2 ------- COMMENT: f9b779cdcdfe781f 8 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: f9b779cdcdfe781f 9 OBFd5QQGQsatgICaJMrqFN+R61Y figure 1B ------- COMMENT: f9b779cdcdfe781f 11 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: f9b779cdcdfe781f 14 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: f9b779cdcdfe781f 15 Pqma8SQG+WyKDfYUrrBnBS2VTlI Fig. 7B ------- COMMENT: f9b779cdcdfe781f 16 wr5TXzzUQ5a8pjtOfW1cRWvVFaY Fig. 7D ------- COMMENT: f9b779cdcdfe781f 17 wr5TXzzUQ5a8pjtOfW1cRWvVFaY Fig. 7D ------- COMMENT: f9b779cdcdfe781f 20 XFKOvIXBUTYdwC7QiiXrJfZl/Y8 (comment: checkpoint) ------- COMMENT: f9e73b547427ce13 3 +PZyH0FFfEYOEFcx9E2e/ykrDX8 loss of Mdi1 did not impact Dnm1 recruitment to mitochondria and Dnm1 foci appeared associated with the hyperfused net structures (Fig. 4 F, arrows). ------- COMMENT: f9e73b547427ce13 4 kgvnA1iZsDRRnCutwSWp+48vRgY Thus, our data indicate that Mdi1 plays a conserved role as a profission factor that is not required for Dnm1 recruitment but is required to facilitate the completion of mitochondrial division. Fig 4 ------- COMMENT: f9e73b547427ce13 5 TMK+Y4mPoZ1Aace0o8o9q5eGKYg Figure 4G ------- COMMENT: f9e73b547427ce13 6 Sm7dHaLqQObOJhvVvBFPHGSeBnE Figure 4F (comment: mitochondrial net-like morphology) ------- COMMENT: f9e73b547427ce13 7 vjORFFyA/TF+brw3p2sipv0fxqE Figure 4F (comment: mitochondrial net-like morphology) ------- COMMENT: f9e73b547427ce13 8 TMK+Y4mPoZ1Aace0o8o9q5eGKYg Figure 4G ------- COMMENT: fa139ea3d6452102 6 N6OXA01r70L/F8E/56E9tSN1MqA (comment: assayed for bulk poly(A)+ RNA) ------- COMMENT: fa139ea3d6452102 10 jPoomAWytk3P1S2mVMaxJXiWl9Y (comment: arrest point determined by H1 kinase activity peak) ------- COMMENT: fa139ea3d6452102 16 N6OXA01r70L/F8E/56E9tSN1MqA (comment: assayed for bulk poly(A)+ RNA) ------- COMMENT: fa1ed8446f4571f5 1 gyiLWCn6NxJajHiU/kX8pY66mI4 (Fig. 3C, 3D) ------- COMMENT: fa1ed8446f4571f5 2 gyiLWCn6NxJajHiU/kX8pY66mI4 (Fig. 3C, 3D) ------- COMMENT: fa1ed8446f4571f5 3 d8xLp/dA0M0C7hDu7v7MWXmIJX4 (Fig. 4C, 4D) ------- COMMENT: fa1ed8446f4571f5 4 d8xLp/dA0M0C7hDu7v7MWXmIJX4 (Fig. 4C, 4D) ------- COMMENT: fa1ed8446f4571f5 5 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: fa1ed8446f4571f5 6 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: fa1ed8446f4571f5 7 tdY+sTkcVXQXSK7tyg52iDlFg+Y (Fig. S1A) ------- COMMENT: fa1ed8446f4571f5 8 tdY+sTkcVXQXSK7tyg52iDlFg+Y (Fig. S1A) ------- COMMENT: fa1ed8446f4571f5 9 2SzzzO7fhvsfj6BW9v5jsOqwj/4 (Fig. S3) ------- COMMENT: fa1ed8446f4571f5 10 2SzzzO7fhvsfj6BW9v5jsOqwj/4 (Fig. S3) ------- COMMENT: fa1ed8446f4571f5 11 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: fa1ed8446f4571f5 12 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: fa1ed8446f4571f5 13 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: fa1ed8446f4571f5 14 682OUPbRs6swFlfqnlWx8UUZl0E (Fig. 1C) ------- COMMENT: fa1ed8446f4571f5 15 8loUy7fe0QOMk5ONEZM8D8QaT+A (Fig. 2A) ------- COMMENT: fa1ed8446f4571f5 16 tdY+sTkcVXQXSK7tyg52iDlFg+Y (Fig. S1A) ------- COMMENT: fa1ed8446f4571f5 17 tdY+sTkcVXQXSK7tyg52iDlFg+Y (Fig. S1A) ------- COMMENT: fa1ed8446f4571f5 18 tdY+sTkcVXQXSK7tyg52iDlFg+Y (Fig. S1A) ------- COMMENT: fa1ed8446f4571f5 19 tdY+sTkcVXQXSK7tyg52iDlFg+Y (Fig. S1A) ------- COMMENT: fa1ed8446f4571f5 20 xhsEwSjsS3WiGn8vmKaT8Vr1lUo (Fig. S2) ------- COMMENT: fa2bda2a002ecb74 1 YPiEtwspH4TaKLQOM9yrVgMWh1U and is already defective in actomyosin  17 compaction at the permissive temperature of 24 C (Figure S1C, video S2) and (Figures 3A, 3B, and S3A)) ------- COMMENT: fa2bda2a002ecb74 2 Vb+AwgERNmJGk9DGTG1UXIJfaUs (comment: COMPACTION I asked is this the correct genotype? Dan answered) Our quantification in FigS3C (bottom panel) shows that ring compaction is slightly faster in scs2Δscs22Δmyo2-E1(aaG345R) than wt at 24 degree, although such a difference is not significant quantitatively. Qualitatively, compaction (in terms of mobility) of individual nodes was indeed faster. So, I felt reluctant to use either “normal” or “abnormal” to describe that. ------- COMMENT: fa2bda2a002ecb74 8 kHwBAhNHkBs0Diq+qz4AVDeKiP0 Figures 3A, 3B, and S3A (no rescue) dan says "FigS3A (bottom panel) shows that ring compaction completely failed in scs2Δscs22Δmyo2-E1(aaG345R) at 36 degree. In fact, we did not directly show that these cells failed ring contraction similarly as myo2-E1(aaG345R) at high temperature (which is well known) in the paper, as we mainly focused on ring compaction process. But we have implied that in the context." ------- COMMENT: fa2bda2a002ecb74 9 BkRCIQ4Bev1kcYf0YcHb19Bhr/Y Figure 3 (comment: COMPACTION) ------- COMMENT: fa2bda2a002ecb74 12 ThxehxHc/T1PefTNH9dUkYjPjpI (comment: CHECK partial rescue) Incomplete ring compaction was still observed in myo2- E1pil1D, although such fraction was reduced as compared with myo2-E1 (Figure 3B I don't see the images - this is from the bar chart)) ------- COMMENT: fa2bda2a002ecb74 15 ZjDClv6W8Sv3laYc0UFnY7pvkHE (comment: COMPACTION )Figures 3A, 3B, and S3A (inhibiting exocytosis rescues defect of compaction) ------- COMMENT: fa2bda2a002ecb74 16 2ZNioh6u8cfAuvXkn6SkCsWw3ZQ (comment: COMPACTION) Figures 3A, 3B, and S3A ------- COMMENT: fa2bda2a002ecb74 17 H6o1YTu0tHopE6EbDvwyEzCMFu4 figure 4D ------- COMMENT: fa2bda2a002ecb74 18 H6o1YTu0tHopE6EbDvwyEzCMFu4 figure 4D ------- COMMENT: fa51a7649e995191 2 +I2RuepoM5loF5En4rSN880xuEQ (comment: CHECK interacts with unmodified Ase1 PR:000027520) ------- COMMENT: fa51a7649e995191 5 x9OWaeB0NVjOwN8+Y7nT+UxSgrE (comment: CHECK interphase, prophase, metaphase,anaphase A) ------- COMMENT: fa51a7649e995191 6 YV1BqS5rjblqk3tnHJy44gYEt3o (comment: CHECK mitotic anaphase B) ------- COMMENT: fa51a7649e995191 8 kmPbgOtJZMfA/s8ajnZ0ilNTaBs fig 1e ------- COMMENT: fa51a7649e995191 9 kmPbgOtJZMfA/s8ajnZ0ilNTaBs fig 1e ------- COMMENT: fa51a7649e995191 10 kmPbgOtJZMfA/s8ajnZ0ilNTaBs fig 1e ------- COMMENT: fa51a7649e995191 11 kmPbgOtJZMfA/s8ajnZ0ilNTaBs fig 1e ------- COMMENT: fa51a7649e995191 12 kmPbgOtJZMfA/s8ajnZ0ilNTaBs fig 1e ------- COMMENT: fa51a7649e995191 13 kmPbgOtJZMfA/s8ajnZ0ilNTaBs fig 1e ------- COMMENT: fa51a7649e995191 14 kmPbgOtJZMfA/s8ajnZ0ilNTaBs fig 1e ------- COMMENT: fa51a7649e995191 16 jZPC0hqnLugDlKWXofg60A9F2oU (comment: with monopolar spindle) ------- COMMENT: fa51a7649e995191 22 2hLZmQcbrb+8T9BpHdf7iIMZGb4 (comment: CHECK mitotic anaphase B, mitotic telophase) ------- COMMENT: fa51a7649e995191 30 ePp0YOfOTv2D+J9pccopDWRT6v0 fig 4a ------- COMMENT: fa51a7649e995191 31 ePp0YOfOTv2D+J9pccopDWRT6v0 fig 4a ------- COMMENT: fa51a7649e995191 33 TjtCAQjujdvi+gBYXjP7mJtN+Os (comment: cdc2 dependent phophorylation) (fig. 4B) ------- COMMENT: fa51a7649e995191 34 OkaFxo6Z00/qfRiDrOY2exes4jg (comment: cdc2 dependent phophorylation) (fig. 5B) ------- COMMENT: fa51a7649e995191 43 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: fa51a7649e995191 44 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: fa51a7649e995191 45 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: fa51a7649e995191 46 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: fa51a7649e995191 49 rtGcSnJlWStlCS7ulg3mIl6G1tI Figure 3D ------- COMMENT: fa51a7649e995191 50 rtGcSnJlWStlCS7ulg3mIl6G1tI Figure 3D ------- COMMENT: fa51a7649e995191 51 rtGcSnJlWStlCS7ulg3mIl6G1tI Figure 3D ------- COMMENT: fa51a7649e995191 52 rtGcSnJlWStlCS7ulg3mIl6G1tI Figure 3D ------- COMMENT: fa51a7649e995191 53 rtGcSnJlWStlCS7ulg3mIl6G1tI Figure 3D ------- COMMENT: fa51a7649e995191 55 EbIarRaVdDp8WyTCxs0s8tT+Itg (comment: CHECK interacts with unmodified Klp9 PR:000027705) ------- COMMENT: fa7f755050797623 8 JRUBkJ8h6WlN4w2QgUZJxT23hUs (comment: CONDITION non fermentable carbon source) ------- COMMENT: fa7f755050797623 9 aGW70ovB3ZfLKL41t2fXKz0/Y8w (comment: happens during mitotic M phase? term will be renames "mitochondrial membrane fission") ------- COMMENT: fa7f755050797623 10 cuIN3a7h2mTdQ8W30506mLaDq/Y (comment: CHECK during mitotic M phase?) ------- COMMENT: fa89b880e6cd4f8d 8 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 9 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 10 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 11 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 12 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 13 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 14 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 15 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 16 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 17 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 18 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 19 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 20 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 21 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 22 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 23 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 24 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 25 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 26 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 27 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 28 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 29 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 30 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 31 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 32 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 33 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 34 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 35 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 36 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 37 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 38 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 39 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 40 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 41 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 42 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 43 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 44 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 45 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 46 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 47 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 48 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 49 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 50 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 51 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 52 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 53 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 54 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 55 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 56 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 57 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 58 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 59 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 60 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 61 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 62 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 63 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 64 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 65 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 66 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 67 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 68 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 69 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 70 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 71 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 72 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 73 CZi1U9dseN1S0mMaEcB0V+2QL8Y Table 2 ------- COMMENT: fa89b880e6cd4f8d 74 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: fa89b880e6cd4f8d 75 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: fa89b880e6cd4f8d 76 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: fa89b880e6cd4f8d 77 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: fa89b880e6cd4f8d 78 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: fa89b880e6cd4f8d 79 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: fa89b880e6cd4f8d 80 7nkFkejREgDThj8O5WegYsaDowo Fig. 4G ------- COMMENT: fa89b880e6cd4f8d 81 7nkFkejREgDThj8O5WegYsaDowo Fig. 4G ------- COMMENT: fa8f1b63230268b1 1 CyrHjDTe/A7ZA7HfpkI+wznRVDE (comment: together with nse1 and nse3 in four-component Two-hybrid) ------- COMMENT: fa8f1b63230268b1 2 esFTm18IUWVrX8pLoxisjaTOAik Figure 2E (comment: tetrad analysis) ------- COMMENT: fa8f1b63230268b1 4 UHhu48pFHzCpYowcqAAVMPzG2z0 (comment: together with nse6 in three-component Two-hybrid) ------- COMMENT: fa8f1b63230268b1 5 X5gxyTfzP8gS+pAPa9xYuT2Dldw (comment: together with nse1 in three-component Two-hybrid) ------- COMMENT: fa8f1b63230268b1 6 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: fa8f1b63230268b1 9 F5Ro5Gc4KTMdMGrPF+J2Y+oo1+k (comment: together with nse4 in three-component Two-hybrid) ------- COMMENT: fa8f1b63230268b1 11 dtAdtlWTN9mWwD2hRRdToqhmqRc (comment: together with nse1-nse3-nse4 in five-component Two-hybrid) ------- COMMENT: fa8f1b63230268b1 22 Hv234uXXLApbZzmjBTK8gB4NMAg (comment: linker) ------- COMMENT: fae46ef295058751 13 oIjVYLV8yuaFNzOKOxOIjAysqd4 (comment: temperature sensitive 37°) ------- COMMENT: fb4d3db1b9346bf1 2 1aJWo8wyH2z5A0E4FFKTSZLrDzU Fig. 5E, 5F from paper for partial inhibition protein synthesis phenotype ------- COMMENT: fb4d3db1b9346bf1 5 fKLjAtmYDfpQVM8s/V3KmkUdIog hese observations are consistent with the idea that the essential TORC1 is the major complex responsi- ble for the rapid inhibition of protein synthesis and that changes in phosphorylation levels relevant to regulating protein synthesis should be detectable within 20 min and significant by 40 min of TOR inhibition. ------- COMMENT: fb4d3db1b9346bf1 6 J9tbfyYJVrnuENpPhcjhAY178jE For the Torin1-resistant mutant, the phosphorylation levels remained constant throughout the time course ------- COMMENT: fb4d3db1b9346bf1 8 RkFyhDPEb2ZGvKT7c9ypKFCNEZU This result indicates that the immediate decrease in protein synthesis rates upon TOR inhibition is not dependent upon the S6Ks or their downstream targets. ------- COMMENT: fb4d3db1b9346bf1 9 J9tbfyYJVrnuENpPhcjhAY178jE For the Torin1-resistant mutant, the phosphorylation levels remained constant throughout the time course ------- COMMENT: fb4ffc5eba4ac1e6 2 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: fb4ffc5eba4ac1e6 3 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: fb4ffc5eba4ac1e6 4 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: fb4ffc5eba4ac1e6 5 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: fb4ffc5eba4ac1e6 6 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: fb4ffc5eba4ac1e6 7 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: fb4ffc5eba4ac1e6 8 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: fb4ffc5eba4ac1e6 9 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: fb4ffc5eba4ac1e6 12 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: fb4ffc5eba4ac1e6 13 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: fb4ffc5eba4ac1e6 16 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: fb4ffc5eba4ac1e6 17 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: fb4ffc5eba4ac1e6 19 9ydzgNyB4udJzLP4/IT5/w5/jCY fig 2 ------- COMMENT: fb4ffc5eba4ac1e6 20 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: fb4ffc5eba4ac1e6 21 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: fb4ffc5eba4ac1e6 22 UJPS51Lvf6oqW/AleuSecGirPSk fig 4 (comment: CHECK 60%) ------- COMMENT: fb4ffc5eba4ac1e6 23 Es+xzvz+7BCXCX3DZ2ohHKxlkC0 fig 4 (comment: CHECK 40% act remaining) ------- COMMENT: fb81220ce61d5104 1 jaWfKYax6qbaGz39vlwtzjlIgrw (comment: CHECK This interaction depends on the phosphorylation of Crb2 on the T73 and S80 residues.) ------- COMMENT: fb81220ce61d5104 78 jaWfKYax6qbaGz39vlwtzjlIgrw (comment: CHECK This interaction depends on the phosphorylation of Crb2 on the T73 and S80 residues.) ------- COMMENT: fb81220ce61d5104 81 jaWfKYax6qbaGz39vlwtzjlIgrw (comment: CHECK This interaction depends on the phosphorylation of Crb2 on the T73 and S80 residues.) ------- COMMENT: fb815dc7d9f5a139 1 Ox0zWHmLLmJAEZoByyYPgBb0dS4 (comment: Phenotype determined with robotics-based CFU assay.) ------- COMMENT: fb815dc7d9f5a139 2 Ox0zWHmLLmJAEZoByyYPgBb0dS4 (comment: Phenotype determined with robotics-based CFU assay.) ------- COMMENT: fb815dc7d9f5a139 3 Ox0zWHmLLmJAEZoByyYPgBb0dS4 (comment: Phenotype determined with robotics-based CFU assay.) ------- COMMENT: fb815dc7d9f5a139 4 Ox0zWHmLLmJAEZoByyYPgBb0dS4 (comment: Phenotype determined with robotics-based CFU assay.) ------- COMMENT: fb815dc7d9f5a139 5 Ox0zWHmLLmJAEZoByyYPgBb0dS4 (comment: Phenotype determined with robotics-based CFU assay.) ------- COMMENT: fb815dc7d9f5a139 6 Ox0zWHmLLmJAEZoByyYPgBb0dS4 (comment: Phenotype determined with robotics-based CFU assay.) ------- COMMENT: fb815dc7d9f5a139 7 Ox0zWHmLLmJAEZoByyYPgBb0dS4 (comment: Phenotype determined with robotics-based CFU assay.) ------- COMMENT: fbade2fc32e2063d 19 COpkedeYb1yodq60e55rbGmdKbo (comment: only required when there are problems , possibly involved in repair of monoorientation) ------- COMMENT: fc07dc88b6ff742e 7 B5qr9IgGOrsCv0YScpH5KwaG3yU (comment: both partners cyr1delta) ------- COMMENT: fc448a004fc8f2f8 8 3xMoreTSf64rAIhAhXxjcZ10xqI (comment: if it is there after cytokinesis + during anaphase B, then I guess it is safe to say that it is there during cytokinesis too..) ------- COMMENT: fc448a004fc8f2f8 48 taWF0JyvqwBykt67PY61cwKksRQ (comment: seems to play a minor role - ppk11 physically interacts with pmo25. ppk11 deletion mutants have less pmo25 at the cell division site. This is not so important under optimal conditions but becomes important when cells are stressed. The phenotype of MOR mutants is excaberated by ppk11-delta.) ------- COMMENT: fc448a004fc8f2f8 49 taWF0JyvqwBykt67PY61cwKksRQ (comment: seems to play a minor role - ppk11 physically interacts with pmo25. ppk11 deletion mutants have less pmo25 at the cell division site. This is not so important under optimal conditions but becomes important when cells are stressed. The phenotype of MOR mutants is excaberated by ppk11-delta.) ------- COMMENT: fc448a004fc8f2f8 50 taWF0JyvqwBykt67PY61cwKksRQ (comment: seems to play a minor role - ppk11 physically interacts with pmo25. ppk11 deletion mutants have less pmo25 at the cell division site. This is not so important under optimal conditions but becomes important when cells are stressed. The phenotype of MOR mutants is excaberated by ppk11-delta.) ------- COMMENT: fc488fcc50f328f6 46 VO2BD/bzXx7FUdvSHJRL+WnxWrE (comment: with cut at second division) ------- COMMENT: fc488fcc50f328f6 57 mkg7qmY0l/9fn+oDUrBocTBZ+BA (comment: with cut) ------- COMMENT: fc488fcc50f328f6 63 JTDVY6lIRQABpYtkBNZGLhCQ4tY (comment: after passage through G1) ------- COMMENT: fc488fcc50f328f6 73 tnIQQ1fqjN7Fch/QF8t7mLA+vtI (comment: CHECK SAC- fypo/issues/2310) ------- COMMENT: fc76162e4e6ace33 1 gcYbNeW9ABwJzM/w9LoSIykmnFQ Table S3; spore viability similar to wild type ------- COMMENT: fc76162e4e6ace33 3 gcYbNeW9ABwJzM/w9LoSIykmnFQ Table S3; spore viability similar to wild type ------- COMMENT: fc76162e4e6ace33 4 gcYbNeW9ABwJzM/w9LoSIykmnFQ Table S3; spore viability similar to wild type ------- COMMENT: fc76162e4e6ace33 5 gcYbNeW9ABwJzM/w9LoSIykmnFQ Table S3; spore viability similar to wild type ------- COMMENT: fc76162e4e6ace33 6 gcYbNeW9ABwJzM/w9LoSIykmnFQ Table S3; spore viability similar to wild type ------- COMMENT: fc76162e4e6ace33 7 gcYbNeW9ABwJzM/w9LoSIykmnFQ Table S3; spore viability similar to wild type ------- COMMENT: fc76162e4e6ace33 8 vC/46FXyy0cPpYlsRnfgm2a7xfk Table S3; spore viability lower than wild type (~50% of wild-type viability) ------- COMMENT: fc76162e4e6ace33 9 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: fc76162e4e6ace33 11 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: fc76162e4e6ace33 12 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: fc76162e4e6ace33 13 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: fc76162e4e6ace33 14 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: fc76162e4e6ace33 15 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: fc76162e4e6ace33 16 JoPiOV/9XpK3GIgz7TH1v3e0Gzk Table S3 ------- COMMENT: fcfd9a50a6a6cb53 1 IhzJRV0JadN0Dn9rpzbsnhVjG4U (comment: CONDITION 20°) ------- COMMENT: fcfd9a50a6a6cb53 2 IhzJRV0JadN0Dn9rpzbsnhVjG4U (comment: CONDITION 20°) ------- COMMENT: fcfd9a50a6a6cb53 3 IhzJRV0JadN0Dn9rpzbsnhVjG4U (comment: CONDITION 20°) ------- COMMENT: fcfd9a50a6a6cb53 4 IhzJRV0JadN0Dn9rpzbsnhVjG4U (comment: CONDITION 20°) ------- COMMENT: fcfd9a50a6a6cb53 5 IhzJRV0JadN0Dn9rpzbsnhVjG4U (comment: CONDITION 20°) ------- COMMENT: fcfd9a50a6a6cb53 143 BNpJoNzMonpE+Z7EkhAfii50qFk Neither the N-terminal segment from aa 1–496 nor the C-terminal fragment from 578–710 was able to bind to Dis2 or Swd22 in the 2-hybrid format (Fig 11A) ------- COMMENT: fcfd9a50a6a6cb53 144 BNpJoNzMonpE+Z7EkhAfii50qFk Neither the N-terminal segment from aa 1–496 nor the C-terminal fragment from 578–710 was able to bind to Dis2 or Swd22 in the 2-hybrid format (Fig 11A) ------- COMMENT: fd0faf273f8c3391 1 ZLN/5o1WiYQjPJSR6vNQoRS/rUs the absence of MICOS subunits caused characteristic alterations in mitochondrial morphology (Figure 1A). ------- COMMENT: fd0faf273f8c3391 2 m0/TzWN6Humoq7/zfAxntmFyJPA disruption of cristae architecture through loss of the core MICOS subunit Mic60 caused a growth defect specifically on media that re- quires respiration (Figure 1D). ------- COMMENT: fd0faf273f8c3391 3 ZLN/5o1WiYQjPJSR6vNQoRS/rUs the absence of MICOS subunits caused characteristic alterations in mitochondrial morphology (Figure 1A). ------- COMMENT: fd0faf273f8c3391 4 ZLN/5o1WiYQjPJSR6vNQoRS/rUs the absence of MICOS subunits caused characteristic alterations in mitochondrial morphology (Figure 1A). ------- COMMENT: fd0faf273f8c3391 5 ZLN/5o1WiYQjPJSR6vNQoRS/rUs the absence of MICOS subunits caused characteristic alterations in mitochondrial morphology (Figure 1A). ------- COMMENT: fd0faf273f8c3391 6 HCBYmYQo7GUiBwqQqZhtFu+X9A4 Approximately half of the Dmmc1 cells had abnormal mito- chondrial morphology, and many cells contained the lamellar and ring-shaped mitochondria characteristic of cells with MICOS sub- unit deletions (Figures 2H and 2I) ------- COMMENT: fd0faf273f8c3391 7 m0/TzWN6Humoq7/zfAxntmFyJPA disruption of cristae architecture through loss of the core MICOS subunit Mic60 caused a growth defect specifically on media that re- quires respiration (Figure 1D). ------- COMMENT: fd0faf273f8c3391 8 m0/TzWN6Humoq7/zfAxntmFyJPA disruption of cristae architecture through loss of the core MICOS subunit Mic60 caused a growth defect specifically on media that re- quires respiration (Figure 1D). ------- COMMENT: fd0faf273f8c3391 9 m0/TzWN6Humoq7/zfAxntmFyJPA disruption of cristae architecture through loss of the core MICOS subunit Mic60 caused a growth defect specifically on media that re- quires respiration (Figure 1D). ------- COMMENT: fd0faf273f8c3391 13 thnSK6271oCfNzDBBdWhwD9CaMs Both Mic60 and Mic26 appeared in a semi-punctate pattern distributed throughout the mitochondrial network (Fig- ure 1E). These localization data are similar to observations in both budding yeast and human cells,26,38 suggesting that MICOS complexes also concentrate at cristae junctions in fission yeast. ------- COMMENT: fd0faf273f8c3391 14 thnSK6271oCfNzDBBdWhwD9CaMs Both Mic60 and Mic26 appeared in a semi-punctate pattern distributed throughout the mitochondrial network (Fig- ure 1E). These localization data are similar to observations in both budding yeast and human cells,26,38 suggesting that MICOS complexes also concentrate at cristae junctions in fission yeast. ------- COMMENT: fd0faf273f8c3391 15 b3mowph9+R7uIjmI1ecpNkJN+Ns As expected, all core MICOS subunits were identified among the top hits by both Mic60 and Mic26 (Figures 1F and 1G, blue). ------- COMMENT: fd0faf273f8c3391 16 b3mowph9+R7uIjmI1ecpNkJN+Ns As expected, all core MICOS subunits were identified among the top hits by both Mic60 and Mic26 (Figures 1F and 1G, blue). ------- COMMENT: fd0faf273f8c3391 17 b3mowph9+R7uIjmI1ecpNkJN+Ns As expected, all core MICOS subunits were identified among the top hits by both Mic60 and Mic26 (Figures 1F and 1G, blue). ------- COMMENT: fd0faf273f8c3391 18 b3mowph9+R7uIjmI1ecpNkJN+Ns As expected, all core MICOS subunits were identified among the top hits by both Mic60 and Mic26 (Figures 1F and 1G, blue). ------- COMMENT: fd0faf273f8c3391 19 AkbU1oVMAKd/Xkt1tacQXot269Q and correspond- ingly, both Sam50 and the SAM subunit Metaxin 1 (Mtx1) were also identified as top interactors (Figures 1F and 1G, magenta ------- COMMENT: fd0faf273f8c3391 20 AkbU1oVMAKd/Xkt1tacQXot269Q and correspond- ingly, both Sam50 and the SAM subunit Metaxin 1 (Mtx1) were also identified as top interactors (Figures 1F and 1G, magenta ------- COMMENT: fd0faf273f8c3391 21 AkbU1oVMAKd/Xkt1tacQXot269Q and correspond- ingly, both Sam50 and the SAM subunit Metaxin 1 (Mtx1) were also identified as top interactors (Figures 1F and 1G, magenta ------- COMMENT: fd0faf273f8c3391 22 AkbU1oVMAKd/Xkt1tacQXot269Q and correspond- ingly, both Sam50 and the SAM subunit Metaxin 1 (Mtx1) were also identified as top interactors (Figures 1F and 1G, magenta ------- COMMENT: fd0faf273f8c3391 23 VNAP3h7df8BS+4Kk9eTodeAqlKs Additionally, the MIB component DNAJC11,7 while not expressed in budding yeast, has a paralog in S. pombe and was also identi- fied by both proteins. ------- COMMENT: fd0faf273f8c3391 24 VNAP3h7df8BS+4Kk9eTodeAqlKs Additionally, the MIB component DNAJC11,7 while not expressed in budding yeast, has a paralog in S. pombe and was also identi- fied by both proteins. ------- COMMENT: fd0faf273f8c3391 25 WBxRkFkRwXPQYmx57x8adD3HRao including subunits of the OMM and IMM translocons (TOM and TIM complexes), prohibi- tins, porin (Por1), the i-AAA protease (Yme1), and OXPHOS proteins such as ATP synthase subunits and NADH dehydroge- nase (Nde1) ------- COMMENT: fd0faf273f8c3391 26 WBxRkFkRwXPQYmx57x8adD3HRao including subunits of the OMM and IMM translocons (TOM and TIM complexes), prohibi- tins, porin (Por1), the i-AAA protease (Yme1), and OXPHOS proteins such as ATP synthase subunits and NADH dehydroge- nase (Nde1) ------- COMMENT: fd0faf273f8c3391 27 WBxRkFkRwXPQYmx57x8adD3HRao including subunits of the OMM and IMM translocons (TOM and TIM complexes), prohibi- tins, porin (Por1), the i-AAA protease (Yme1), and OXPHOS proteins such as ATP synthase subunits and NADH dehydroge- nase (Nde1) ------- COMMENT: fd0faf273f8c3391 28 WBxRkFkRwXPQYmx57x8adD3HRao including subunits of the OMM and IMM translocons (TOM and TIM complexes), prohibi- tins, porin (Por1), the i-AAA protease (Yme1), and OXPHOS proteins such as ATP synthase subunits and NADH dehydroge- nase (Nde1) ------- COMMENT: fd0faf273f8c3391 29 WBxRkFkRwXPQYmx57x8adD3HRao including subunits of the OMM and IMM translocons (TOM and TIM complexes), prohibi- tins, porin (Por1), the i-AAA protease (Yme1), and OXPHOS proteins such as ATP synthase subunits and NADH dehydroge- nase (Nde1) ------- COMMENT: fd0faf273f8c3391 30 WBxRkFkRwXPQYmx57x8adD3HRao including subunits of the OMM and IMM translocons (TOM and TIM complexes), prohibi- tins, porin (Por1), the i-AAA protease (Yme1), and OXPHOS proteins such as ATP synthase subunits and NADH dehydroge- nase (Nde1) ------- COMMENT: fd0faf273f8c3391 31 WBxRkFkRwXPQYmx57x8adD3HRao including subunits of the OMM and IMM translocons (TOM and TIM complexes), prohibi- tins, porin (Por1), the i-AAA protease (Yme1), and OXPHOS proteins such as ATP synthase subunits and NADH dehydroge- nase (Nde1) ------- COMMENT: fd0faf273f8c3391 32 WBxRkFkRwXPQYmx57x8adD3HRao including subunits of the OMM and IMM translocons (TOM and TIM complexes), prohibi- tins, porin (Por1), the i-AAA protease (Yme1), and OXPHOS proteins such as ATP synthase subunits and NADH dehydroge- nase (Nde1) ------- COMMENT: fd0faf273f8c3391 33 WBxRkFkRwXPQYmx57x8adD3HRao including subunits of the OMM and IMM translocons (TOM and TIM complexes), prohibi- tins, porin (Por1), the i-AAA protease (Yme1), and OXPHOS proteins such as ATP synthase subunits and NADH dehydroge- nase (Nde1) ------- COMMENT: fd0faf273f8c3391 34 WBxRkFkRwXPQYmx57x8adD3HRao including subunits of the OMM and IMM translocons (TOM and TIM complexes), prohibi- tins, porin (Por1), the i-AAA protease (Yme1), and OXPHOS proteins such as ATP synthase subunits and NADH dehydroge- nase (Nde1) ------- COMMENT: fd0faf273f8c3391 35 WBxRkFkRwXPQYmx57x8adD3HRao including subunits of the OMM and IMM translocons (TOM and TIM complexes), prohibi- tins, porin (Por1), the i-AAA protease (Yme1), and OXPHOS proteins such as ATP synthase subunits and NADH dehydroge- nase (Nde1) ------- COMMENT: fd0faf273f8c3391 36 6fnyvIB8yOguYRGqZ8liDhlaxbI Interestingly, we noticed that one of the top interac- tors identified by proteomic analysis of both Mic60 and Mic26 was the poorly characterized protein Mmc1 (Figures 1F and 1G, red). ------- COMMENT: fd0faf273f8c3391 37 6fnyvIB8yOguYRGqZ8liDhlaxbI Interestingly, we noticed that one of the top interac- tors identified by proteomic analysis of both Mic60 and Mic26 was the poorly characterized protein Mmc1 (Figures 1F and 1G, red). ------- COMMENT: fd0faf273f8c3391 38 sP6Stz4l2h19owtGxR4GkZZJQrA Mmc1-FLAG was detected in the purified mitochondria by western analysis and was protected from Proteinase K digestion, suggesting the protein indeed lo- calizes to mitochondria (Figure 2B ------- COMMENT: fd0faf273f8c3391 39 ZE+/XqJWuSNU8vQgv9LujTnVCtI Instead, Mmc1-FLAG was only degraded after the combined addition of Proteinase K and the detergent Triton X-100, suggesting that the C terminus of Mmc1 localizes to the matrix. Mmc1 was found in the pellet fraction, indicating that it is an integral membrane protein (Figure 2C). ------- COMMENT: fd0faf273f8c3391 40 Hzf1egB9/zNKXjk6S7/JXV9t698 . These data, in combination with previous immuno-EM-labeling experi- ments of MICOS subunits,31,38 suggest that Mmc1 concentrates at cristae junctions, where it associates in proximity with the MICOS complex. ------- COMMENT: fd0faf273f8c3391 41 iigAwjFp+DI9XJ5+hJZTfIpHOjk . Together, these data indicate that Mmc1 is a MICOS complex-associated protein required for normal cristae morphology. ------- COMMENT: fd0faf273f8c3391 42 vr8DIplSsXeK7qqkPNn6AZ/Qo/M (comment: CHECK ******NEW TERM REQUIRED ******)In contrast, in the absence of Mic10 and Mic26, which are constituents of a second MICOS subcomplex, Mmc1 lost its semi-punctate appearance and was more uniformly localized throughout the mitochondrial network (Figures 3A and 3B). ------- COMMENT: fd0faf273f8c3391 43 vr8DIplSsXeK7qqkPNn6AZ/Qo/M (comment: CHECK ******NEW TERM REQUIRED ******)In contrast, in the absence of Mic10 and Mic26, which are constituents of a second MICOS subcomplex, Mmc1 lost its semi-punctate appearance and was more uniformly localized throughout the mitochondrial network (Figures 3A and 3B). ------- COMMENT: fd0faf273f8c3391 44 ZLN/5o1WiYQjPJSR6vNQoRS/rUs the absence of MICOS subunits caused characteristic alterations in mitochondrial morphology (Figure 1A). ------- COMMENT: fd0faf273f8c3391 45 ZLN/5o1WiYQjPJSR6vNQoRS/rUs the absence of MICOS subunits caused characteristic alterations in mitochondrial morphology (Figure 1A). ------- COMMENT: fd0faf273f8c3391 46 ETXyBtmyKhXnDwNSUfXb9PKrmfA Strikingly, we observed a severe synthetic growth defect specifically on respiration-requiring media in the absence of Mmc1 and MICOS subunits (Figure 5D). ------- COMMENT: fd0faf273f8c3391 47 /m70b/G5H9c9qCyX/D/u03kSzQg (comment: complex member) ------- COMMENT: fd0faf273f8c3391 48 /m70b/G5H9c9qCyX/D/u03kSzQg (comment: complex member) ------- COMMENT: fd0faf273f8c3391 49 /m70b/G5H9c9qCyX/D/u03kSzQg (comment: complex member) ------- COMMENT: fd35ea91d23f5b2a 2 Sk3BWLRWSFqwM6J+hpx0tRCFu50 (comment: G2 temperature shift) ------- COMMENT: fd35ea91d23f5b2a 3 Myp7TnDkgovWJ4p+wy/Po/OlGVs (comment: G1 temperature shift) ------- COMMENT: fd35ea91d23f5b2a 5 5z/fBF6Ht9xa37i3b9gDXJvryMc (comment: in arrested cells, indicating independent of cell cycle progression) ------- COMMENT: fd4f3f52f1d38106 14 QBzxQ6Okwdn08dooQ2f3cWe/nj4 ATPase domain mutant phenotype fig 5 and S6 ------- COMMENT: fd7d19b1aed14f7c 1 0wmKbzSwqS9VMdXr9EaaGOfoD0M (comment: CHECK normal oxygen level) ------- COMMENT: fd7d19b1aed14f7c 2 0wmKbzSwqS9VMdXr9EaaGOfoD0M (comment: CHECK normal oxygen level) ------- COMMENT: fd98ac563a6f67cc 1 h7ZsRbN/AWGCaJ3Adz6i7NcQxDo expressed Clostridium botulinum C3 protein to ADP-ribosylate Rho proteins including Rho1 ------- COMMENT: fdc3419730dab35f 1 6RhCzeJfWGMvdRNj8WKcAJ/9ipM (comment: CHECK (requested negative regulation of) synonym mitotic telomere dispersion during metaphase) ------- COMMENT: fdc3419730dab35f 2 qFMkwRUk+awea+IfkPWEiftfmjA (Fig. 2A, 0–3 min, arrows) ------- COMMENT: fdc3419730dab35f 3 Q0AhViLfxeG0amf4KAoGoxLYtK0 (comment: telomere disjunction) ------- COMMENT: fdc3419730dab35f 4 TB/6UPjnZJIzZZPH8++zD2fxd9g Fig. S2A, right ------- COMMENT: fdc3419730dab35f 5 i0+18r9T8ljIC0pPSZbg6on8a7M Fig 2C, 2D ------- COMMENT: fdc3419730dab35f 6 z1sja1Xcp4dzk1f2v443NaOUlEI Fig 2C, 2D (comment: decreased telomere dispersion) ------- COMMENT: fdc3419730dab35f 7 ydaU/PBxwwbn75pTND6x70f4omk Fig 2D ------- COMMENT: fdc3419730dab35f 8 4uF9ny9/6JS94LCFegwT9vyY3RY fig 4 ------- COMMENT: fdc3419730dab35f 9 XOM/1214VG5hXuhFIXA0B6olHJA fig 4 (comment: decreased telomere dispersion) ------- COMMENT: fdc3419730dab35f 10 r9ZEK1cYXudGVkY9Z1C94Gp01DI Fig. S4A ------- COMMENT: fdc3419730dab35f 11 ms4xcN1XBwRU7a2yGgC8Mpu2FGM Fig. 4 (comment: decreased telomere dispersion) ------- COMMENT: fdc3419730dab35f 12 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: fdc3419730dab35f 13 iugwOICngLmkyma0auuN7ZPWnmQ Fig 3D ------- COMMENT: fdc3419730dab35f 14 s+fI9mccOvNsI52hjjqJPzYe/QU Fig 3 ------- COMMENT: fdc3419730dab35f 17 uGQjww+cwaBN/09U9xrc5IaLzoA Fig. 5A ------- COMMENT: fdc3419730dab35f 18 1qVsO7UsmP+f3BsCXyliC8kosAo Together, these experiments suggest that Aurora-dependent re- moval of Swi6/HP1 and consequently cohesin Rad21 from telo- meres in early mitosis contributes to telomere dispersion. ------- COMMENT: fdc3419730dab35f 19 1qVsO7UsmP+f3BsCXyliC8kosAo Together, these experiments suggest that Aurora-dependent re- moval of Swi6/HP1 and consequently cohesin Rad21 from telo- meres in early mitosis contributes to telomere dispersion. ------- COMMENT: fdc3419730dab35f 20 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: fdc3419730dab35f 21 l+f1R919mLn1hkTFl9hvZaQF59A fig6 ------- COMMENT: fdc3419730dab35f 23 8G6+j/Mkk6lmbSbCeunxDRKglvY fig7 ------- COMMENT: fdc8926d3d89d0b4 1 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: fdc8926d3d89d0b4 2 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: fdc8926d3d89d0b4 3 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: fdc8926d3d89d0b4 4 loPA+oLJjtem5pnyK2AKQjoS9iY Figure 2A ------- COMMENT: fdc8926d3d89d0b4 5 AOYuh7dL/YHR7SDOO0sTQx7XCZ4 Figure S1A ------- COMMENT: fdc8926d3d89d0b4 6 AOYuh7dL/YHR7SDOO0sTQx7XCZ4 Figure S1A ------- COMMENT: fdc8926d3d89d0b4 7 rpWIlJtGnbAjy3n+TzmMlldgbBg Figure 3A, 3B . (comment: number and intensity) ------- COMMENT: fdc8926d3d89d0b4 8 S4Qe5ZqD/Pi5EFkrOZAoVMFAPoc Recombination rates were decreased by 10-fold in mto1∆ strains in both recombination substrates (Figure 4B) ------- COMMENT: fdc8926d3d89d0b4 9 S4Qe5ZqD/Pi5EFkrOZAoVMFAPoc Recombination rates were decreased by 10-fold in mto1∆ strains in both recombination substrates (Figure 4B) ------- COMMENT: fdc8926d3d89d0b4 10 L5WZvHeybuieEwzhasX7z6qcP3s Reduced Rad21 binding to chromosome arms ------- COMMENT: fdc8926d3d89d0b4 11 dhxlcnGNrD71bMJUesjb4NINOLw (comment: CHECK issues/3588 decreased) ------- COMMENT: fdc8926d3d89d0b4 12 0+ZZPVNZOmVfZAm7hP3aOQ+2eX4 fig1B–G ------- COMMENT: fdc8926d3d89d0b4 13 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: fdc8926d3d89d0b4 14 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: fdc8926d3d89d0b4 15 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: fdc8926d3d89d0b4 16 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: fdc8926d3d89d0b4 17 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: fdc8926d3d89d0b4 18 ZE8ZBrGmYoVFbJ3YFMLd5XE2NVw fig2 ------- COMMENT: fdc8926d3d89d0b4 19 8/+EdrD9R6b3GfVp6fH+l78PTJQ Figure 2C No increase in severity to mto1 delete ------- COMMENT: fdc8926d3d89d0b4 20 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: fdc8926d3d89d0b4 21 f4wGuR5kDnNBJxOKauzGIxR5BsU Figure 2C ------- COMMENT: fdc8926d3d89d0b4 24 hWO7E5Z5vPhPW+PV6unAVVQM0YM Figure 3A, 3B ------- COMMENT: fdc8926d3d89d0b4 25 l1pfcO1rkUlOVsYVHQG0Rl4s3Cc We examined whether Mto1 localizes to DNA repair factories and found that Mto1-mCherry was not detectable within the nucleus, as previously shown (Sawin et al., 2004; Venkatram et al.) ------- COMMENT: fdc8926d3d89d0b4 26 4l3BGQPQVK9g1rwct/jXaoSGaeQ (comment: CHECK issues/3588) Figure 5A–E ------- COMMENT: fdeaa8d994824f8c 23 XUosipeHUvQkzVbZCpDknh1+wDY (comment: CHECK same as hus1delta alone) ------- COMMENT: fdeaa8d994824f8c 24 cz0cE+B35dEUB6X+dhxRQVsT7gQ (comment: CHECK same as rad9delta alone) ------- COMMENT: fdeaa8d994824f8c 26 XUosipeHUvQkzVbZCpDknh1+wDY (comment: CHECK same as hus1delta alone) ------- COMMENT: fdeaa8d994824f8c 27 cz0cE+B35dEUB6X+dhxRQVsT7gQ (comment: CHECK same as rad9delta alone) ------- COMMENT: fdeaa8d994824f8c 30 XUosipeHUvQkzVbZCpDknh1+wDY (comment: CHECK same as hus1delta alone) ------- COMMENT: fdeaa8d994824f8c 31 cz0cE+B35dEUB6X+dhxRQVsT7gQ (comment: CHECK same as rad9delta alone) ------- COMMENT: fdeaa8d994824f8c 32 XUosipeHUvQkzVbZCpDknh1+wDY (comment: CHECK same as hus1delta alone) ------- COMMENT: fdeaa8d994824f8c 33 cz0cE+B35dEUB6X+dhxRQVsT7gQ (comment: CHECK same as rad9delta alone) ------- COMMENT: fdfc9c73a3820302 1 koKon0B1JS8VjN5euwC6qfZG5+Q Fig. S1. (comment: Genetic screen for mutants defective in heterochromatin propagation.) ------- COMMENT: fdfc9c73a3820302 2 cuFJ2EJMi8B9iBtRB4rNntWth0U The other two mutants showed defective silencing of the mat2P::ura4+ reporter and haploid meiosis (Fig. 4C), as well as detectable levels of the mat2P transcript (Fig. 4D). ------- COMMENT: fdfc9c73a3820302 3 UaNe90OyQi/1a2Vq6o5JnSUvZrM , mcm2-1 and mcl1-4 cells failed to maintain heterochromatin, as indicated by white colony color and loss of the H3K9me3 mark (SI Appendix, Fig. S7). Thus, both Mcl1 and Mcm2 are required for propagation of heterochromatin at an ectopic site. ------- COMMENT: fdfc9c73a3820302 5 cuFJ2EJMi8B9iBtRB4rNntWth0U The other two mutants showed defective silencing of the mat2P::ura4+ reporter and haploid meiosis (Fig. 4C), as well as detectable levels of the mat2P transcript (Fig. 4D). ------- COMMENT: fdfc9c73a3820302 6 UaNe90OyQi/1a2Vq6o5JnSUvZrM , mcm2-1 and mcl1-4 cells failed to maintain heterochromatin, as indicated by white colony color and loss of the H3K9me3 mark (SI Appendix, Fig. S7). Thus, both Mcl1 and Mcm2 are required for propagation of heterochromatin at an ectopic site. ------- COMMENT: fdfc9c73a3820302 16 PaWisCOUYU4T03ghcfzdtO/vDkk (Fig. 4E and F). ------- COMMENT: fdfc9c73a3820302 17 PaWisCOUYU4T03ghcfzdtO/vDkk (Fig. 4E and F). ------- COMMENT: fdfc9c73a3820302 18 PaWisCOUYU4T03ghcfzdtO/vDkk (Fig. 4E and F). ------- COMMENT: fdfc9c73a3820302 19 PaWisCOUYU4T03ghcfzdtO/vDkk (Fig. 4E and F). ------- COMMENT: fdfc9c73a3820302 33 Cv3bqRt6x1nhgS0qWUGaFCjXq5U Fig S1E ------- COMMENT: fdfc9c73a3820302 34 Cv3bqRt6x1nhgS0qWUGaFCjXq5U Fig S1E ------- COMMENT: fdfc9c73a3820302 35 Cv3bqRt6x1nhgS0qWUGaFCjXq5U Fig S1E ------- COMMENT: fdfc9c73a3820302 36 Cv3bqRt6x1nhgS0qWUGaFCjXq5U Fig S1E ------- COMMENT: fdfc9c73a3820302 37 Cv3bqRt6x1nhgS0qWUGaFCjXq5U Fig S1E ------- COMMENT: fdfc9c73a3820302 38 Cv3bqRt6x1nhgS0qWUGaFCjXq5U Fig S1E ------- COMMENT: fdfc9c73a3820302 39 Cv3bqRt6x1nhgS0qWUGaFCjXq5U Fig S1E ------- COMMENT: fdfc9c73a3820302 40 Cv3bqRt6x1nhgS0qWUGaFCjXq5U Fig S1E ------- COMMENT: fdfc9c73a3820302 41 Cv3bqRt6x1nhgS0qWUGaFCjXq5U Fig S1E ------- COMMENT: fdfc9c73a3820302 42 Cv3bqRt6x1nhgS0qWUGaFCjXq5U Fig S1E ------- COMMENT: fdfc9c73a3820302 43 Cv3bqRt6x1nhgS0qWUGaFCjXq5U Fig S1E ------- COMMENT: fdfc9c73a3820302 44 Cv3bqRt6x1nhgS0qWUGaFCjXq5U Fig S1E ------- COMMENT: fdfc9c73a3820302 45 Cv3bqRt6x1nhgS0qWUGaFCjXq5U Fig S1E ------- COMMENT: fdfc9c73a3820302 46 Cv3bqRt6x1nhgS0qWUGaFCjXq5U Fig S1E ------- COMMENT: fdfc9c73a3820302 47 Cv3bqRt6x1nhgS0qWUGaFCjXq5U Fig S1E ------- COMMENT: fdfc9c73a3820302 48 Cv3bqRt6x1nhgS0qWUGaFCjXq5U Fig S1E ------- COMMENT: fdfc9c73a3820302 49 Cv3bqRt6x1nhgS0qWUGaFCjXq5U Fig S1E ------- COMMENT: fdfc9c73a3820302 50 Cv3bqRt6x1nhgS0qWUGaFCjXq5U Fig S1E ------- COMMENT: fdfc9c73a3820302 51 Cv3bqRt6x1nhgS0qWUGaFCjXq5U Fig S1E ------- COMMENT: fdfc9c73a3820302 52 koKon0B1JS8VjN5euwC6qfZG5+Q Fig. S1. (comment: Genetic screen for mutants defective in heterochromatin propagation.) ------- COMMENT: fdfc9c73a3820302 53 koKon0B1JS8VjN5euwC6qfZG5+Q Fig. S1. (comment: Genetic screen for mutants defective in heterochromatin propagation.) ------- COMMENT: fdfc9c73a3820302 54 koKon0B1JS8VjN5euwC6qfZG5+Q Fig. S1. (comment: Genetic screen for mutants defective in heterochromatin propagation.) ------- COMMENT: fdfc9c73a3820302 55 koKon0B1JS8VjN5euwC6qfZG5+Q Fig. S1. (comment: Genetic screen for mutants defective in heterochromatin propagation.) ------- COMMENT: fdfc9c73a3820302 56 koKon0B1JS8VjN5euwC6qfZG5+Q Fig. S1. (comment: Genetic screen for mutants defective in heterochromatin propagation.) ------- COMMENT: fdfc9c73a3820302 57 koKon0B1JS8VjN5euwC6qfZG5+Q Fig. S1. (comment: Genetic screen for mutants defective in heterochromatin propagation.) ------- COMMENT: fdfc9c73a3820302 59 TMVb5R2fhfqwFkm6d33BVQfBAmc Mcl1 localized to heterochromatic loci in S phase, like Mcm2 (Fig. 2B). ------- COMMENT: fdfc9c73a3820302 60 TMVb5R2fhfqwFkm6d33BVQfBAmc Mcl1 localized to heterochromatic loci in S phase, like Mcm2 (Fig. 2B). ------- COMMENT: fdfc9c73a3820302 61 2FVvuIAUeYUadB6VtH3PKUTl42g (Fig. 2D and E and SI Appendix, Figs. S2A–D and S3). Mcm2 mapping was performed 60 min after release from the cdc25-22 block. A similar septation index for WT and swi6Δ cells indicated normal progression of swi6Δ cells through S phase (SI Appendix, Fig. S2B). ------- COMMENT: fdfc9c73a3820302 62 yIxXmc/JvD5VB9WtOcqQg9WvxWo Compared to WT, swi6Δ cells showed a considerable decrease in BrdU incorporation across mat, which persisted through the time course (Fig. 2D and SI Appendix, Fig. S3). Mcm2 was still detected, although with more defined peaks, likely reflecting licensed, but not activated, replication ori­ gins across the domain (Fig. 2D) ------- COMMENT: fdfc9c73a3820302 63 C5jxs56ojSt/EpUMWMWUKOKjMwU The mcm2-6 mutant showed sensitivity to hydroxyurea (HU) (SI Appendix, Fig. S5C), indicating defective replication, and was not further analyzed. ------- COMMENT: fdfc9c73a3820302 66 +c+5pwSbMW2IUEQSqTiHM3+sFc0 Notably, the addition of the WT Mcm2 HBD during the purification of the H3 and H4 histones promoted their solubili­ zation, highlighting its role as a histone chaperone (SI Appendix, Fig. S5D). ------- COMMENT: fdfc9c73a3820302 67 fn0MCtWEpC8YZiPhIkywXtLbOS8 Thus, although both mutants affect the retention of parental histones, Mcl1 plays a more critical role in the process. ------- COMMENT: fdfc9c73a3820302 70 r6vVjFOKIZSxhW0RWF8jPILl54U FACT associates with subunits of MCM (Mcm2) and GINS (Psf3) when Mcl1 is present, but these interactions are lost in cells lacking Mcl1 (Fig. 6A), ------- COMMENT: fdfc9c73a3820302 71 r6vVjFOKIZSxhW0RWF8jPILl54U FACT associates with subunits of MCM (Mcm2) and GINS (Psf3) when Mcl1 is present, but these interactions are lost in cells lacking Mcl1 (Fig. 6A), ------- COMMENT: fe0f450d0b971f5a 2 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: fe0f450d0b971f5a 3 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: fe0f450d0b971f5a 4 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: fe0f450d0b971f5a 5 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: fe0f450d0b971f5a 6 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: fe0f450d0b971f5a 7 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: fe0f450d0b971f5a 8 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: fe0f450d0b971f5a 9 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: fe0f450d0b971f5a 10 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: fe0f450d0b971f5a 11 kmgZ9PABctlh4ZQgKZknkfSX+BA Fig. 1 ------- COMMENT: fe0f450d0b971f5a 12 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: fe0f450d0b971f5a 13 Mij9SBEPt4GIt/vtUBP2T7kXS+o Fig. 3B, 3C ------- COMMENT: fe0f450d0b971f5a 14 EYJvgheVgDxo4q6sDbxkashegrQ Fig. 3 ------- COMMENT: fe0f450d0b971f5a 15 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: fe0f450d0b971f5a 16 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: fe0f450d0b971f5a 17 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: fe0f450d0b971f5a 18 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: fe0f450d0b971f5a 19 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: fe0f450d0b971f5a 20 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: fe0f450d0b971f5a 21 6Z8l4l78OPOa9U/pQCisNMwSvis Fig. 4A ------- COMMENT: fe0f450d0b971f5a 22 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: fe0f450d0b971f5a 23 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: fe0f450d0b971f5a 24 ImPdCjxoyC+fRwZDSDOQKZLDFbs Rng3p-GFP3 and Rng3p-YFP3 concentrated in contractile rings from anaphase B through constriction (Fig. 5). ------- COMMENT: fe0f450d0b971f5a 25 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: fe0f450d0b971f5a 26 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: fe0f450d0b971f5a 27 uQnDT/kh2//7DQk9Tc69KhaGQmQ Fig. 6B ------- COMMENT: fe0f450d0b971f5a 28 mTKN91KXXisZB5ma3BhRc4KFuGc Fig. 6C ------- COMMENT: fe0f450d0b971f5a 29 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: fe0f450d0b971f5a 30 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: fe0f450d0b971f5a 31 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: fe0f450d0b971f5a 32 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: fe0f450d0b971f5a 33 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: fe0f450d0b971f5a 34 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: fe0f450d0b971f5a 35 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: fe0f450d0b971f5a 36 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: fe0f450d0b971f5a 37 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: fe0f450d0b971f5a 38 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: fe0f450d0b971f5a 39 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: fe0f450d0b971f5a 40 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: fe0f450d0b971f5a 41 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: fe0f450d0b971f5a 42 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: fe0f450d0b971f5a 43 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: fe11aad740874c4e 1 bohDSRdUFOhYmkhMTmcmaQGBR7Y fig4 ------- COMMENT: fe12cd716f83c472 1 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: fe12cd716f83c472 2 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: fe12cd716f83c472 3 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: fe12cd716f83c472 4 C4tzGFvuNcZlPaiOStbp+5u5nTM Fig. 3B ------- COMMENT: fe12cd716f83c472 5 lLgIJkP89AfTDrtMImKTheeRbRw Fig. 4 ------- COMMENT: fe12cd716f83c472 6 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: fe12cd716f83c472 7 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: fe12cd716f83c472 8 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: fe12cd716f83c472 9 w66qKz2iII3JIfMsyMn4vUVrdfY Fig. 6A ------- COMMENT: fe12cd716f83c472 10 gtSghehrp0Y6rqNtUSBzAqV7igo Fig. 7 ------- COMMENT: fe12cd716f83c472 11 nSIxHD8Ksw3BcaIvdfiWytx5qiY We found that Slk1 was also localized to the SPB during metaphase II and anaphase II. Fig. 7 ------- COMMENT: fe12cd716f83c472 12 JUKiF37j64aMaKpSH0OBgfVgnXQ Fig. 8A ------- COMMENT: fe12cd716f83c472 13 Q500UYQGWDo7n4siJbO2OPeMGhw Fig. 8 ------- COMMENT: fe12cd716f83c472 14 xrje/svuSIR6RYxUhnSJS3oEZxw Fig. 9 ------- COMMENT: fe6e8e353ea78411 1 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: fe6e8e353ea78411 3 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: fe6e8e353ea78411 4 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: fe6e8e353ea78411 5 /+IaLOUfKNRmR4TjzfV+lQxmplE The same level of Myo2p co-immunoprecipitated with mutant Cdc4p as with wild-type Cdc4p (Fig. 5A). ------- COMMENT: fe6e8e353ea78411 9 PdwhseqlrW5Xuya8UXCRybLS6G0 DNS ------- COMMENT: fe724bae9eef58f0 3 q6aiDg/1LPIUHa/NMqpVymyoMyk (comment: assayed using cell extract, overexpressed protien and synthetic UB conjugate) ------- COMMENT: fe7a0f32d4e76d5d 3 qZES7TyNAgCzbCkiVHnIhigMjYg Fig. 5A ------- COMMENT: fe7a0f32d4e76d5d 6 oXrahwH/EUTq3pU54FPDXMsc++Y fig1 ------- COMMENT: fe7a0f32d4e76d5d 11 798VTMP3bU64iDGmhbgifPrhj68 Fig. S2B ------- COMMENT: fe7a0f32d4e76d5d 13 V8aY9cMSuflWPDnZXK1Ej8D/EEk fig 1b ------- COMMENT: fe7a0f32d4e76d5d 14 f1Pnp7eGl9Rl/3ivt2LqfUU0otY Fig. S3 ------- COMMENT: fe7a0f32d4e76d5d 15 YSqAwKUdlvRoS56GwBFzo+lDt+o (comment: added affected genes as extensions) fig 3A-C ------- COMMENT: fe7a0f32d4e76d5d 26 TvAwuR+dxlZpGG1EOd0Geht4eXI Fig. S2A, S1E ------- COMMENT: fe7a0f32d4e76d5d 214 lHjaiZ8kn9BowO2OAF8EIJBBbEw (comment: vw: moved down to elongated (update fypo?)) fig 1e ------- COMMENT: fe7a0f32d4e76d5d 215 jzxAgfmwHa2f4Uiad0niwB/nLxM fig 1a (comment: + others) ------- COMMENT: fe7a0f32d4e76d5d 218 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 219 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 220 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 221 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 222 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 223 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 224 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 225 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 226 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 227 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 228 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 229 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 230 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 231 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 232 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 233 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 234 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 235 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 236 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 237 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 238 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 239 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 240 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 241 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 242 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 243 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 244 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 245 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 246 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 247 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 248 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 249 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 250 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 251 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 252 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 253 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 254 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 255 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 256 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 257 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 258 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 259 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 260 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 261 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 262 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 263 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 264 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 265 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 266 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 267 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 268 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 269 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 270 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 271 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 272 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 273 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 274 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 275 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 276 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 277 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 278 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 279 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 280 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 281 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 282 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 283 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 284 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 285 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 286 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 287 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 288 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 289 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 290 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 291 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 292 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 293 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 294 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 295 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 296 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 297 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 298 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 299 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 300 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 301 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 302 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 303 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 304 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 305 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 306 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 307 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 308 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 309 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 310 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 311 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 312 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 313 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 314 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 315 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 316 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 317 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 322 e7BfQLtAPO9qG9Ox8ykS+TURKpg (comment: Ndc80-GFP) ------- COMMENT: fe7a0f32d4e76d5d 323 0w49AWkpg+7GDlxnbH6LJ69kQ+A (comment: Nuf2-GFP) ------- COMMENT: fe7a0f32d4e76d5d 326 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 328 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 329 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 330 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 331 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 332 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 333 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 334 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 335 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 336 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 337 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 338 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 339 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 340 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 341 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 342 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 343 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 344 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 345 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 346 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 347 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 348 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 349 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 350 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 351 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 352 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 353 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 354 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 355 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 356 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 357 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 358 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 359 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 360 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 361 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 362 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 363 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 364 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 365 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 366 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 367 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 368 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 369 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 370 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 371 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 372 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 373 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 374 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 375 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 376 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 377 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 378 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 379 PiOZVq0kUmzYCix9Nscux7d0iQ4 (comment: CHECK in the presence of 10 µg/ml Thiabendazole) ------- COMMENT: fe7a0f32d4e76d5d 381 LkGQQw8FQBPnHuF6zSR3Jquhnkc fig 1C ------- COMMENT: fe7a0f32d4e76d5d 382 l3ai4330ma8wv/meVtk5HDXhjHQ fig 1 ------- COMMENT: fe7a0f32d4e76d5d 383 EK3cJaIpApHaZfDj/il8fbVV8BY fig S2 ------- COMMENT: fe7a0f32d4e76d5d 384 Mj4Jap10vtVXQtC8IWxZvmTJ5Js Fig. s3C ------- COMMENT: fe7a0f32d4e76d5d 385 myfVODOjZuSlazo4cNlJgSxQodk Fig. s3E ------- COMMENT: fe7a0f32d4e76d5d 386 6pcOFxH9HzTf3s3kUiydCn0s/vo Fig. S3G, S3H ------- COMMENT: fe7a0f32d4e76d5d 387 Wfzt9oatmukS2lBPp5C5Nn3/BKo Fig. S3I (comment: additive, do we know %?) ------- COMMENT: fe7a0f32d4e76d5d 388 QAAZGJnKZpVywMOxmORJl2ftQCw (comment: added affected genes as extensions) fig 3E ------- COMMENT: fe7a0f32d4e76d5d 389 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: fe7a0f32d4e76d5d 390 +fbc2jd5eOwf9994skILYf5dKqk fig 3 ------- COMMENT: fe7a0f32d4e76d5d 391 6eCM6XdQuzQghhOr7ri+Fp6qqRY fig 3G ------- COMMENT: fe7a0f32d4e76d5d 392 J0aqpbBvwP2S79icCGoEZimYMvY fig 4a ------- COMMENT: fe7a0f32d4e76d5d 393 s7cvhfiYiveaAA2KzWLwMqq+fAs fig 4c ------- COMMENT: fe7a0f32d4e76d5d 394 vCZ5/ANd+bkBNZRJfFC3rhm5IwY fig 4e ------- COMMENT: fe7a0f32d4e76d5d 395 YtRf4lQsUpdDWmnaJF1GnnUEN20 (Fig. 4G and see the Ubiquitin pull-down section of Materials and methods). ------- COMMENT: fe7a0f32d4e76d5d 396 qZES7TyNAgCzbCkiVHnIhigMjYg Fig. 5A ------- COMMENT: fe7a0f32d4e76d5d 397 Ld/rlfzoQNBqRUODp/DP/AZJPMc fig 5b (comment: nuclear envelope) ------- COMMENT: fe7a0f32d4e76d5d 398 4yCVBSkl6bg2aTrDdCZ1ZS1USlw fig 5b ------- COMMENT: fe7a0f32d4e76d5d 399 qYSY4G7xYV3JCtBTo/UbymvpPCg fig 5D-G (comment: nuclear envelope) ------- COMMENT: fe7a0f32d4e76d5d 400 qYSY4G7xYV3JCtBTo/UbymvpPCg fig 5D-G (comment: nuclear envelope) ------- COMMENT: fe7a0f32d4e76d5d 401 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: fe7a0f32d4e76d5d 402 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: fe7a0f32d4e76d5d 403 kxbbzOkbVGSYlpIRcHR5pGkWb9Y fig 6 ------- COMMENT: fe7a0f32d4e76d5d 404 sBNmtNgFvy88X/4RDO33gTgRnKw fig 2E ------- COMMENT: fe7a0f32d4e76d5d 405 sBNmtNgFvy88X/4RDO33gTgRnKw fig 2E ------- COMMENT: fe7a0f32d4e76d5d 406 sBNmtNgFvy88X/4RDO33gTgRnKw fig 2E ------- COMMENT: fe7a0f32d4e76d5d 407 sBNmtNgFvy88X/4RDO33gTgRnKw fig 2E ------- COMMENT: fe7a0f32d4e76d5d 409 sBNmtNgFvy88X/4RDO33gTgRnKw fig 2E ------- COMMENT: fe7a0f32d4e76d5d 410 sBNmtNgFvy88X/4RDO33gTgRnKw fig 2E ------- COMMENT: fe7a0f32d4e76d5d 411 sBNmtNgFvy88X/4RDO33gTgRnKw fig 2E ------- COMMENT: fe7a0f32d4e76d5d 412 sBNmtNgFvy88X/4RDO33gTgRnKw fig 2E ------- COMMENT: fe9525dbeb7dffdb 1 LYaTlPkDz5+Y9jGgHcMbeL01qLQ Fig. 1B ------- COMMENT: fe9525dbeb7dffdb 2 QE+Djgc/CpJOdIWcdtfFvfKu1ZA Fig. 1D ------- COMMENT: fe9525dbeb7dffdb 3 gapUQWX9HWfl5CZR6q0wL70TB/0 Fig. 1E ------- COMMENT: fe9525dbeb7dffdb 4 2hmI+aLN1rvviWhZEQNEMoS1fWU Fig. 1F ------- COMMENT: fe9525dbeb7dffdb 5 CCb3rNRrnvNJW1hgcL0apQ1pVrU Fig. 1G ------- COMMENT: fe9525dbeb7dffdb 6 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: fe9525dbeb7dffdb 7 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: fe9525dbeb7dffdb 8 0RmuUsxiXAkyset+jG5tYpg84Jo Fig. 2B ------- COMMENT: fe9525dbeb7dffdb 9 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: fe9525dbeb7dffdb 10 eEnwLBfLOoSdV6NzLJkAt7INPEY Fig. 2D ------- COMMENT: fe9525dbeb7dffdb 11 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: fe9525dbeb7dffdb 12 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: fe9525dbeb7dffdb 13 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: fe9525dbeb7dffdb 14 stkIyotfxuZOGOUCbZKPLYl+haA Fig. 3A ------- COMMENT: fe9525dbeb7dffdb 15 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: fe9525dbeb7dffdb 16 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: fe9525dbeb7dffdb 17 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: fe9525dbeb7dffdb 18 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: fe9525dbeb7dffdb 19 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: fe9525dbeb7dffdb 20 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: fe9525dbeb7dffdb 21 Mij9SBEPt4GIt/vtUBP2T7kXS+o Fig. 3B, 3C ------- COMMENT: fe9525dbeb7dffdb 22 Mij9SBEPt4GIt/vtUBP2T7kXS+o Fig. 3B, 3C ------- COMMENT: fe9525dbeb7dffdb 23 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: fe9525dbeb7dffdb 24 +CTUUApxO2b3+ywDmgC+KL4mfCA Fig. 3C ------- COMMENT: fe9525dbeb7dffdb 25 Mij9SBEPt4GIt/vtUBP2T7kXS+o Fig. 3B, 3C ------- COMMENT: fe9525dbeb7dffdb 26 Mij9SBEPt4GIt/vtUBP2T7kXS+o Fig. 3B, 3C ------- COMMENT: fe9525dbeb7dffdb 27 AXZgGuqG3Hx+AYIo7T4C1AeLDRM Fig. 3D ------- COMMENT: fe9525dbeb7dffdb 28 CH7x2HqL2HW3kbQhKHOMZIM1iLM Ace2p-Myc13 was periodically produced through the cell cycle (Fig. 4A). It began to accumulate during anaphase as determined by the coincidence of binucleate formation, and it peaked in abundance concomitantly with the peak of septation. ------- COMMENT: fe9525dbeb7dffdb 29 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: fe9525dbeb7dffdb 30 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: fe9525dbeb7dffdb 31 vkT8uMpiBxgGlmYXEA8V3+LXigM Fig. 4E ------- COMMENT: fe9525dbeb7dffdb 32 9Xb+uz4ftlqagKGp7TY1gJc7WdA Fig. 4F ------- COMMENT: fe9525dbeb7dffdb 33 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: fe9525dbeb7dffdb 34 o2ALmyccfFgn6OMhFbqXt8V9YEo Fig. 5C ------- COMMENT: fea9b843c477c2cf 255 tk8SlQG/svd5Z7V1V37R0oS1hkY (comment: Trx1's involvement in tis process is to recycle mxr1 for met-O conversion to met ) ------- COMMENT: feac1fe40f21f7a9 46 gbsEZDL2WsTbMBJ8nTkh0vGNYlA fig 2A ------- COMMENT: ff16cae20b63db89 6 TSqE9v45NjCdAC8Q/awbd9XieNc loss of telomeric and subtelomeric sequences at high temperature ------- COMMENT: ff16cae20b63db89 9 MlrxTEMKetT6AeDpW2i+AG/58Ng (comment: CHECK Sensitive to HU, CPT and MMS) ------- COMMENT: ff16cae20b63db89 13 fClhqjGDpW8pbGLyXGkjZOO41Pk (comment: Exacerbated at high temperature) ------- COMMENT: ff16cae20b63db89 46 rZyN9EGitKPNUxbMtzZTOAMrejk (comment: CHECK same as stn1-226 alone) ------- COMMENT: ff16cae20b63db89 78 tV5WUYaTOanvTOHolG3ZV75IE6k (comment: CHECK slightly better growth than stn1-226 alone) ------- COMMENT: ff16db44d9ab6882 34 liwgYY40DKZHu0X3Ekrk9q9wyMo (comment: CHECK PR:000037081= tropomyosin cdc8, acetylated form (fission yeast)) ------- COMMENT: ff16db44d9ab6882 35 9+qJlngtdpctac9VwDvytiRAenY (comment: CHECK GO:0051329= mitotic interphase) ------- COMMENT: ff16db44d9ab6882 39 7clXhKqedKjhh79bq3thmb5J5fA (comment: CHECK acetylated Cdc82 so could use PR:000037081) ------- COMMENT: ff16db44d9ab6882 48 0WmX1dlLvDRgR+r2yTSRDm91+EQ (comment: vw: I used "added by naa20 which is the catalytic subunit for naa25") ------- COMMENT: ff1e67fe7824549f 1 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: ff1e67fe7824549f 2 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: ff1e67fe7824549f 3 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: ff1e67fe7824549f 4 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: ff1e67fe7824549f 5 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: ff1e67fe7824549f 6 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: ff1e67fe7824549f 7 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: ff1e67fe7824549f 11 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: ff1e67fe7824549f 12 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: ff1e67fe7824549f 13 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: ff1e67fe7824549f 14 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: ff1e67fe7824549f 15 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: ff1e67fe7824549f 16 /2SKKP/EJWyU1m3EEAEMflrJbME Figure 3A, 9C ------- COMMENT: ff1e67fe7824549f 17 9+LYdI+SE0vMxdcZa39lWc+7ACY Figure 3A ------- COMMENT: ff1e67fe7824549f 18 xeP3xnEJu0ioxk564y/4MWJd9dA Figure 3B, Figure 10 ------- COMMENT: ff1e67fe7824549f 19 PKz3h3bzqGhc0gkEj8JFDo/VMZ8 Figure 3B, Figure 10 ------- COMMENT: ff1e67fe7824549f 20 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: ff1e67fe7824549f 21 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: ff1e67fe7824549f 22 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: ff1e67fe7824549f 23 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: ff1e67fe7824549f 24 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: ff1e67fe7824549f 25 w2rvd8orCbma2VlWOn/0Y4nPFJ0 Figure 3B ------- COMMENT: ff1e67fe7824549f 26 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: ff1e67fe7824549f 27 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: ff1e67fe7824549f 28 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: ff1e67fe7824549f 29 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: ff1e67fe7824549f 30 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: ff1e67fe7824549f 31 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: ff1e67fe7824549f 32 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: ff1e67fe7824549f 33 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: ff1e67fe7824549f 34 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: ff1e67fe7824549f 35 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: ff1e67fe7824549f 36 2bHlKRtVyVwowEeX9JW3PYUB3Kg Figure 5A ------- COMMENT: ff1e67fe7824549f 37 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: ff1e67fe7824549f 38 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: ff1e67fe7824549f 39 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: ff1e67fe7824549f 40 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: ff1e67fe7824549f 41 RiIMEM/dC2nncpO7JMd8oVaZjdc Figure 5B ------- COMMENT: ff1e67fe7824549f 42 5Leuj0nQtQ/oH58v8XLkYXSDMog Figure 6B ------- COMMENT: ff1e67fe7824549f 43 5Leuj0nQtQ/oH58v8XLkYXSDMog Figure 6B ------- COMMENT: ff1e67fe7824549f 44 5Leuj0nQtQ/oH58v8XLkYXSDMog Figure 6B ------- COMMENT: ff1e67fe7824549f 45 5Leuj0nQtQ/oH58v8XLkYXSDMog Figure 6B ------- COMMENT: ff1e67fe7824549f 46 5Leuj0nQtQ/oH58v8XLkYXSDMog Figure 6B ------- COMMENT: ff1e67fe7824549f 47 bQrAvw5v3pdeGEjTasNZSNQl2dw Figure 6A ------- COMMENT: ff1e67fe7824549f 48 bQrAvw5v3pdeGEjTasNZSNQl2dw Figure 6A ------- COMMENT: ff1e67fe7824549f 49 bQrAvw5v3pdeGEjTasNZSNQl2dw Figure 6A ------- COMMENT: ff1e67fe7824549f 50 bQrAvw5v3pdeGEjTasNZSNQl2dw Figure 6A ------- COMMENT: ff1e67fe7824549f 51 bQrAvw5v3pdeGEjTasNZSNQl2dw Figure 6A ------- COMMENT: ff1e67fe7824549f 52 5Leuj0nQtQ/oH58v8XLkYXSDMog Figure 6B ------- COMMENT: ff1e67fe7824549f 53 5Leuj0nQtQ/oH58v8XLkYXSDMog Figure 6B ------- COMMENT: ff1e67fe7824549f 54 5Leuj0nQtQ/oH58v8XLkYXSDMog Figure 6B ------- COMMENT: ff1e67fe7824549f 55 5Leuj0nQtQ/oH58v8XLkYXSDMog Figure 6B ------- COMMENT: ff1e67fe7824549f 56 5Leuj0nQtQ/oH58v8XLkYXSDMog Figure 6B ------- COMMENT: ff1e67fe7824549f 57 W8U2HOh5c9FHrXYcx0iyvAr6hdQ Figure 7A ------- COMMENT: ff1e67fe7824549f 58 0DxIqxxvbMI3bt2djLmgXdfLzCA Figure 7A, pin∆ rescues the lethality of aps1∆ asp1-H397A ------- COMMENT: ff1e67fe7824549f 59 lewWnS5goUq8jT/WZM96TE1+g7Q Figure 7B ------- COMMENT: ff1e67fe7824549f 60 W8U2HOh5c9FHrXYcx0iyvAr6hdQ Figure 7A ------- COMMENT: ff1e67fe7824549f 61 W8U2HOh5c9FHrXYcx0iyvAr6hdQ Figure 7A ------- COMMENT: ff1e67fe7824549f 62 xCbKZnANhFixEzZd8f2L384Cqpk Figure 8A ------- COMMENT: ff1e67fe7824549f 63 xCbKZnANhFixEzZd8f2L384Cqpk Figure 8A ------- COMMENT: ff1e67fe7824549f 64 xCbKZnANhFixEzZd8f2L384Cqpk Figure 8A ------- COMMENT: ff1e67fe7824549f 65 xCbKZnANhFixEzZd8f2L384Cqpk Figure 8A ------- COMMENT: ff1e67fe7824549f 66 xCbKZnANhFixEzZd8f2L384Cqpk Figure 8A ------- COMMENT: ff1e67fe7824549f 67 +HVzmDDMpGztyycYN8+dVwiyKIw Figure 8B ------- COMMENT: ff1e67fe7824549f 68 +HVzmDDMpGztyycYN8+dVwiyKIw Figure 8B ------- COMMENT: ff1e67fe7824549f 69 +HVzmDDMpGztyycYN8+dVwiyKIw Figure 8B ------- COMMENT: ff1e67fe7824549f 70 +HVzmDDMpGztyycYN8+dVwiyKIw Figure 8B ------- COMMENT: ff1e67fe7824549f 71 +HVzmDDMpGztyycYN8+dVwiyKIw Figure 8B ------- COMMENT: ff1e67fe7824549f 72 +HVzmDDMpGztyycYN8+dVwiyKIw Figure 8B ------- COMMENT: ff1e67fe7824549f 73 dF1tWhz40zsyJsCNZJJ04uBXTfI Figure 7B, 8B ------- COMMENT: ff1e67fe7824549f 74 lewWnS5goUq8jT/WZM96TE1+g7Q Figure 7B ------- COMMENT: ff1e67fe7824549f 75 xWKbLOQNoVQZAbKW+4qIUyl1neI Figure 9C ------- COMMENT: ff1e67fe7824549f 76 xWKbLOQNoVQZAbKW+4qIUyl1neI Figure 9C ------- COMMENT: ff1e67fe7824549f 77 xWKbLOQNoVQZAbKW+4qIUyl1neI Figure 9C ------- COMMENT: ff1e67fe7824549f 78 xWKbLOQNoVQZAbKW+4qIUyl1neI Figure 9C ------- COMMENT: ff1e67fe7824549f 79 nsjl/AexxpV5l54QnCPuNaqTUqc Figure 9B ------- COMMENT: ff1e67fe7824549f 80 nsjl/AexxpV5l54QnCPuNaqTUqc Figure 9B ------- COMMENT: ff1e67fe7824549f 81 nsjl/AexxpV5l54QnCPuNaqTUqc Figure 9B ------- COMMENT: ff1e67fe7824549f 82 nsjl/AexxpV5l54QnCPuNaqTUqc Figure 9B ------- COMMENT: ff1e67fe7824549f 83 nsjl/AexxpV5l54QnCPuNaqTUqc Figure 9B ------- COMMENT: ff1e67fe7824549f 84 VWRY+xIXdxFg+BJJ5YcgBRZgJC4 Figure 10 ------- COMMENT: ff1e67fe7824549f 85 VWRY+xIXdxFg+BJJ5YcgBRZgJC4 Figure 10 ------- COMMENT: ff1e67fe7824549f 86 QXwpxNSZnmuCFdAqD7OOCpjwHc0 Figure 11 ------- COMMENT: ff1e67fe7824549f 87 QXwpxNSZnmuCFdAqD7OOCpjwHc0 Figure 11 ------- COMMENT: ff1e67fe7824549f 88 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: ff1e67fe7824549f 89 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: ff1e67fe7824549f 90 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: ff1e67fe7824549f 91 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: ff1e67fe7824549f 92 7iCVfcHB9m+mnL6v0sfvN4W+yeo Figure S1 ------- COMMENT: ff1e67fe7824549f 93 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: ff1e67fe7824549f 94 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: ff1e67fe7824549f 95 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: ff1e67fe7824549f 96 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: ff1e67fe7824549f 97 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: ff1e67fe7824549f 98 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: ff1e67fe7824549f 99 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: ff1e67fe7824549f 100 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: ff1e67fe7824549f 101 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: ff1e67fe7824549f 102 Q/yZ2Wn0G4QVlJkX6kV9Ryx2IMk Figure S2 ------- COMMENT: ff1e67fe7824549f 103 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: ff1e67fe7824549f 104 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: ff1e67fe7824549f 105 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: ff1e67fe7824549f 106 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: ff1e67fe7824549f 107 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: ff1e67fe7824549f 108 crdk8+5Su6ZjOoDfvYBv9bmPLs0 Figure 5C ------- COMMENT: ff1e67fe7824549f 109 W8U2HOh5c9FHrXYcx0iyvAr6hdQ Figure 7A ------- COMMENT: ff1e67fe7824549f 110 W8U2HOh5c9FHrXYcx0iyvAr6hdQ Figure 7A ------- COMMENT: ff1e67fe7824549f 111 nsjl/AexxpV5l54QnCPuNaqTUqc Figure 9B ------- COMMENT: ff1e67fe7824549f 112 nsjl/AexxpV5l54QnCPuNaqTUqc Figure 9B ------- COMMENT: ff1e67fe7824549f 113 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: ff1e67fe7824549f 114 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: ff1e67fe7824549f 115 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: ff1e67fe7824549f 116 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: ff1e67fe7824549f 117 yuNVP4Py+pFHADHg/jOTuTXWcUs Figure S3 ------- COMMENT: ff1e67fe7824549f 118 E4rYcahCrgwRxAsYYVHY/2vQNVQ Figure S7 ------- COMMENT: ff1e67fe7824549f 119 E4rYcahCrgwRxAsYYVHY/2vQNVQ Figure S7 ------- COMMENT: ff1e67fe7824549f 120 7zwUg5GRKIbiAMjaZc4jHMdcNKo Figure 1 ------- COMMENT: ff703973c23246bb 20 KtZ6rnJ7eKlWTpaJbSqGsXndZ5I fig5 ------- COMMENT: ff759b3e7d7da717 1 oeP1+2g2H11j0yINB4IqNgSouyk Fig. 1A indicating that CDK1 activity remained high ------- COMMENT: ff759b3e7d7da717 2 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: ff759b3e7d7da717 3 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: ff759b3e7d7da717 4 uX2urvOiyxYrDK5l6UPoN6tKDNI Fig. 1B, ------- COMMENT: ff759b3e7d7da717 5 gkdwXZkmzTcoN1cwix9jmiBbHkc Fig. 1A ------- COMMENT: ff759b3e7d7da717 6 qg28PmK6CVKAOdsAN0v5ZvjUrAo Fig. 1D, 1E ------- COMMENT: ff759b3e7d7da717 7 bpFttvDS4VeEVcUvQcOpfSEJjew Fig. 2A, , (comment: complex seen here in anaphase although it normally forms in prometaphase and disassembles before anaphase) ------- COMMENT: ff759b3e7d7da717 8 JDje8AanoR6DlB1CaOryw/c0f2o Fig. 2B, 2C ------- COMMENT: ff759b3e7d7da717 9 JDje8AanoR6DlB1CaOryw/c0f2o Fig. 2B, 2C ------- COMMENT: ff759b3e7d7da717 10 zwER1c+kXT2WQNhHCWn09py6af0 Fig. 3A, 3B, 3E, securin abnormally stabilized during anaphase ------- COMMENT: ff759b3e7d7da717 11 S917w+ibEUyBYQon4hH0V1JgDEA Fig. S1B, S1C ------- COMMENT: ff759b3e7d7da717 12 4cbqhematx1kQz0J39lYeJEIW88 figure S1B ------- COMMENT: ff80aaf19b5e7f04 2 +RfldMv9O+DXQL661U1MNefO9Ys uridylation prevents excessive deadenylation which in turn protects mRNA from 3’ to 5’ exonucleolitic decay. ------- COMMENT: ff80aaf19b5e7f04 4 atvymUD8/nbMNvX55OloPA4Ux4U mRNAs with shortened poly(A) tails are uridylated by Cid1, while completely deadenylated mRNAs are subjected to oligouridylation by Cid16. Cid1- mediated uridylation routes decay towards the 5’ to 3’ pathway, while Cid16-mediated oligouridylation facilitates 3’ to 5’ degradation. ------- COMMENT: ff80aaf19b5e7f04 6 SW8GuPwTQ6KV2a4oC5TKK8xi+Rs Under standard conditions, uridylation of short mRNA poly(A) tails by Cid1 helps to direct mRNA toward the 5’ to 3’ decay pathway by enhancing Lsm1–7 complex binding and protecting the 3’-end from extensive deadenylation. ------- COMMENT: ff80aaf19b5e7f04 12 BIZ1HQQ+kwYAY3pJQ6P7eJOLPO0 (comment: CHECK ******uridylation dependent 3'-5' mRNA decay*******.) (Fig. 5) ------- COMMENT: ff80aaf19b5e7f04 14 omDV8wJmdRcNaQ3tnrRz6JlhvTw (Fig. 3A and B and Fig. S4) ------- COMMENT: ff80aaf19b5e7f04 15 +RzAJhKT3hEIV/mD9vxVXP49yaw (Fig. 3A and B and Fig. S4B) ------- COMMENT: ff80aaf19b5e7f04 16 l3uUZp3hSNNsjzgve++aBByv0Wo (Fig. 3C, Fig. S4) ------- COMMENT: ff80aaf19b5e7f04 17 ZHQqVhNwVOeb/xSJmcePvWUVWFk (Fig. 3C, Fig. S4B) ------- COMMENT: ff80aaf19b5e7f04 18 0ioefkFyvW/WmkzwNc9/2j/m270 (Fig. S5B) ------- COMMENT: ff80aaf19b5e7f04 19 0ioefkFyvW/WmkzwNc9/2j/m270 (Fig. S5B) ------- COMMENT: ff80aaf19b5e7f04 20 0ioefkFyvW/WmkzwNc9/2j/m270 (Fig. S5B) ------- COMMENT: ff80aaf19b5e7f04 21 EPnEauOBelIZRHVBgX+EdXa8kW8 (Fig. 4A, 4F) ------- COMMENT: ff80aaf19b5e7f04 22 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: ff80aaf19b5e7f04 23 iJ3g3vWtuBePHc8K3s12w3Wmanw (Fig. 4B, 4F) ------- COMMENT: ff80aaf19b5e7f04 24 OLX2XbBiXXL//akQ08vXiP/Q8NI (Fig. 4B) ------- COMMENT: ff80aaf19b5e7f04 25 d8xLp/dA0M0C7hDu7v7MWXmIJX4 (Fig. 4C, 4D) ------- COMMENT: ff80aaf19b5e7f04 26 d8xLp/dA0M0C7hDu7v7MWXmIJX4 (Fig. 4C, 4D) ------- COMMENT: ff80aaf19b5e7f04 27 d8xLp/dA0M0C7hDu7v7MWXmIJX4 (Fig. 4C, 4D) ------- COMMENT: ff80aaf19b5e7f04 28 d8xLp/dA0M0C7hDu7v7MWXmIJX4 (Fig. 4C, 4D) ------- COMMENT: ff80aaf19b5e7f04 29 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: ff80aaf19b5e7f04 30 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: ff80aaf19b5e7f04 31 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: ff80aaf19b5e7f04 32 f6lHWvpmX1kCpsYm7CvZdsKf484 (Fig. 4F) ------- COMMENT: ff80aaf19b5e7f04 33 dusOI1Nd94uY08sF6eWxbIb4Is0 (Fig. 4A) ------- COMMENT: ff80aaf19b5e7f04 34 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: ff80aaf19b5e7f04 35 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: ff80aaf19b5e7f04 36 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: ff80aaf19b5e7f04 37 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: ff80aaf19b5e7f04 38 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: ff80aaf19b5e7f04 39 Gj9BcilvLffFRjioFwWFeuPsIEU (Fig. 5B) ------- COMMENT: ff80aaf19b5e7f04 40 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: ff80aaf19b5e7f04 41 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: ff80aaf19b5e7f04 42 gduoATehTPmTNhm2JRsAKq34tDE (Fig. 5C) ------- COMMENT: ff80aaf19b5e7f04 43 lzDpBhI+e1KR8hAG7s00IgdEh2I (Fig. 5A) ------- COMMENT: ff80aaf19b5e7f04 44 lzDpBhI+e1KR8hAG7s00IgdEh2I (Fig. 5A) ------- COMMENT: ff80aaf19b5e7f04 45 atvymUD8/nbMNvX55OloPA4Ux4U mRNAs with shortened poly(A) tails are uridylated by Cid1, while completely deadenylated mRNAs are subjected to oligouridylation by Cid16. Cid1- mediated uridylation routes decay towards the 5’ to 3’ pathway, while Cid16-mediated oligouridylation facilitates 3’ to 5’ degradation. ------- COMMENT: ff80aaf19b5e7f04 47 sHpDQ1mOwdbvV2SRFt9UGnHKnGI While the global tail profile did not change in the Δcid16 mutant, we noticed an increase in the fraction of short-tailed reads in Δcid1 and double deletion strains (Fig. 3e). ------- COMMENT: ff80aaf19b5e7f04 48 IgaU6ocMvzSXy6D23HwsY1MywvQ (comment: CHECK ******uridylation dependent 3'-5' mRNA decay******) (Fig. 5) ------- COMMENT: ff80aaf19b5e7f04 49 QjM/i+xEdTb8oE0abBjf1usyLug The highest number of oligo(U) tailed reads were detected for mitochondrial transcripts (Supplementary Fig. 4a), therefore, oligouridylation of mtRNA seems to be a main function of Cid16. ------- COMMENT: ff80aaf19b5e7f04 50 QjM/i+xEdTb8oE0abBjf1usyLug The highest number of oligo(U) tailed reads were detected for mitochondrial transcripts (Supplementary Fig. 4a), therefore, oligouridylation of mtRNA seems to be a main function of Cid16. ------- COMMENT: ff8c38cbaa71124d 18 ROeCx0JTXFQl/rfcZ7X2RPqZhp8 (comment: during mitotic DNA replication initiation) ------- COMMENT: ff9ebc8d76211732 1 VQWlBba+xlIfw+mAiBAm8lq5UXE In the atf1- mutant, transcripts a and b are expressed normally, whereas tran- scripts c and d are absent (Fig. 4A). ------- COMMENT: ff9ebc8d76211732 2 VQWlBba+xlIfw+mAiBAm8lq5UXE In the atf1- mutant, transcripts a and b are expressed normally, whereas tran- scripts c and d are absent (Fig. 4A). ------- COMMENT: ff9ebc8d76211732 3 VQWlBba+xlIfw+mAiBAm8lq5UXE In the atf1- mutant, transcripts a and b are expressed normally, whereas tran- scripts c and d are absent (Fig. 4A). ------- COMMENT: ff9ebc8d76211732 4 VQWlBba+xlIfw+mAiBAm8lq5UXE In the atf1- mutant, transcripts a and b are expressed normally, whereas tran- scripts c and d are absent (Fig. 4A). ------- COMMENT: ff9ebc8d76211732 5 tQVnf81VqqXMR0d1Hc3+M+S24LY In the rst22 mutant, transcripts a, b, and c are expressed normally, whereas transcript d is absent (Fig. 4A). ------- COMMENT: ff9ebc8d76211732 6 tQVnf81VqqXMR0d1Hc3+M+S24LY In the rst22 mutant, transcripts a, b, and c are expressed normally, whereas transcript d is absent (Fig. 4A). ------- COMMENT: ff9ebc8d76211732 7 tQVnf81VqqXMR0d1Hc3+M+S24LY In the rst22 mutant, transcripts a, b, and c are expressed normally, whereas transcript d is absent (Fig. 4A). ------- COMMENT: ff9ebc8d76211732 8 tQVnf81VqqXMR0d1Hc3+M+S24LY In the rst22 mutant, transcripts a, b, and c are expressed normally, whereas transcript d is absent (Fig. 4A). ------- COMMENT: ff9ebc8d76211732 9 7uCAkQ58kIvLOQtHBL6K5hptqS4 Moreover, fbp11 derepression is recovered by deleting both tup111 and tup121, indicating that Atf1 and Rst2 are dispensable for fbp11 induction in the absence of both Tup proteins ------- COMMENT: ff9ebc8d76211732 10 7uCAkQ58kIvLOQtHBL6K5hptqS4 Moreover, fbp11 derepression is recovered by deleting both tup111 and tup121, indicating that Atf1 and Rst2 are dispensable for fbp11 induction in the absence of both Tup proteins ------- COMMENT: ff9ebc8d76211732 11 x7OsApL62jnTb3mRLPW75gJ1cvg Expression of transcript c is not restored in the atf12tup112tup122 mutant, sug- gesting that Atf1 is essential to induce transcript c. ------- COMMENT: ff9ebc8d76211732 12 opUSPBWA3tzTxBlKTQUXn5j+Glo Chromatin remodelling events and RNAPII loading around the TATA box are severely impaired in an atf12 mutant, demonstrating that the progression of ncRNA initi- ation events mediated by Atf1 is essential to convert chromatin to an RNAPII accessible state (Fig. 4B). ------- COMMENT: ffbb86b907aa5e22 1 MGbYa3rq3s+BLF4xd9q9O4UVln0 We found that saf140, saf60, and saf50 are essential genes, as viability segregated 2:2 in four-spore tetrads ------- COMMENT: ffbb86b907aa5e22 2 MGbYa3rq3s+BLF4xd9q9O4UVln0 We found that saf140, saf60, and saf50 are essential genes, as viability segregated 2:2 in four-spore tetrads ------- COMMENT: ffbb86b907aa5e22 3 MGbYa3rq3s+BLF4xd9q9O4UVln0 We found that saf140, saf60, and saf50 are essential genes, as viability segregated 2:2 in four-spore tetrads ------- COMMENT: ffbb86b907aa5e22 4 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: ffbb86b907aa5e22 5 GbhWkoMdEo6hdMaWXJhEEpFM7Bw Fig. 2A ------- COMMENT: ffbb86b907aa5e22 10 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: ffbb86b907aa5e22 11 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: ffbb86b907aa5e22 12 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: ffbb86b907aa5e22 13 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: ffbb86b907aa5e22 14 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: ffbb86b907aa5e22 15 UMog3t75aPlFjEe6Xt8t5IbojjA Fig. 2C ------- COMMENT: ffbb86b907aa5e22 16 TtHKJCZRDpRLoAAOOcgCi+YaLRw In general, we found SAPHIRE distributed widely throughout the genome and dispersed at genes over all three chromosome arms. ------- COMMENT: ffbb86b907aa5e22 17 TtHKJCZRDpRLoAAOOcgCi+YaLRw In general, we found SAPHIRE distributed widely throughout the genome and dispersed at genes over all three chromosome arms. ------- COMMENT: ffbb86b907aa5e22 18 TtHKJCZRDpRLoAAOOcgCi+YaLRw In general, we found SAPHIRE distributed widely throughout the genome and dispersed at genes over all three chromosome arms. ------- COMMENT: ffbb86b907aa5e22 19 TtHKJCZRDpRLoAAOOcgCi+YaLRw In general, we found SAPHIRE distributed widely throughout the genome and dispersed at genes over all three chromosome arms. ------- COMMENT: ffbb86b907aa5e22 20 sIUT7EzYs/uU6V597FEExXMrLII we observed SAPHIRE in the region of the silent mating (MAT) locus ------- COMMENT: ffbb86b907aa5e22 21 sIUT7EzYs/uU6V597FEExXMrLII we observed SAPHIRE in the region of the silent mating (MAT) locus ------- COMMENT: ffbb86b907aa5e22 22 sIUT7EzYs/uU6V597FEExXMrLII we observed SAPHIRE in the region of the silent mating (MAT) locus ------- COMMENT: ffbb86b907aa5e22 23 sIUT7EzYs/uU6V597FEExXMrLII we observed SAPHIRE in the region of the silent mating (MAT) locus ------- COMMENT: ffbb86b907aa5e22 24 LKDZgbDx4Bv8U89LG1TBtYHS19I in peaks in the subtelomeric regions of chromosomes 1 and 2 ------- COMMENT: ffbb86b907aa5e22 25 LKDZgbDx4Bv8U89LG1TBtYHS19I in peaks in the subtelomeric regions of chromosomes 1 and 2 ------- COMMENT: ffbb86b907aa5e22 26 LKDZgbDx4Bv8U89LG1TBtYHS19I in peaks in the subtelomeric regions of chromosomes 1 and 2 ------- COMMENT: ffbb86b907aa5e22 27 LKDZgbDx4Bv8U89LG1TBtYHS19I in peaks in the subtelomeric regions of chromosomes 1 and 2 ------- COMMENT: ffbb86b907aa5e22 28 Rpe87xVLv3MJC0w2R5HQpEEFUk8 In addition, SAPHIRE occupies the junctions between the central region of the centromeres and the dg/dh repeats ------- COMMENT: ffbb86b907aa5e22 29 Rpe87xVLv3MJC0w2R5HQpEEFUk8 In addition, SAPHIRE occupies the junctions between the central region of the centromeres and the dg/dh repeats ------- COMMENT: ffbb86b907aa5e22 30 Rpe87xVLv3MJC0w2R5HQpEEFUk8 In addition, SAPHIRE occupies the junctions between the central region of the centromeres and the dg/dh repeats ------- COMMENT: ffbb86b907aa5e22 31 Rpe87xVLv3MJC0w2R5HQpEEFUk8 In addition, SAPHIRE occupies the junctions between the central region of the centromeres and the dg/dh repeats ------- COMMENT: ffbb86b907aa5e22 32 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: ffbb86b907aa5e22 33 EGAG4JDiP5hWbnaxIkU1KdI4VXY Fig. 4B ------- COMMENT: ffbb86b907aa5e22 34 KOQiSwaOCwPoHQG7M7ZRoaALTFg Fig. 4C ------- COMMENT: ffbb86b907aa5e22 35 mlGxi47yQdG9tnNW3a6SWWSQedE Fig. 5 ------- COMMENT: ffbebba84aad52e5 1 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: ffbebba84aad52e5 2 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: ffbebba84aad52e5 3 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: ffbebba84aad52e5 4 x4SP+2CtWdU2R1zFXNquFb9w754 Table 1 ------- COMMENT: ffbebba84aad52e5 5 baoh2sIwjMXpEVcxfBoBpYi4yqM Figure 1, 2 ------- COMMENT: ffbebba84aad52e5 6 baoh2sIwjMXpEVcxfBoBpYi4yqM Figure 1, 2 ------- COMMENT: ffbebba84aad52e5 7 baoh2sIwjMXpEVcxfBoBpYi4yqM Figure 1, 2 ------- COMMENT: ffbebba84aad52e5 8 baoh2sIwjMXpEVcxfBoBpYi4yqM Figure 1, 2 ------- COMMENT: ffbebba84aad52e5 9 baoh2sIwjMXpEVcxfBoBpYi4yqM Figure 1, 2 ------- COMMENT: ffbebba84aad52e5 10 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: ffbebba84aad52e5 11 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: ffbebba84aad52e5 12 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: ffbebba84aad52e5 13 yJqp00Z+jYrXnh7RRsYc8CriNM8 (comment: *****The definition of this term is not right) Figure 2 ------- COMMENT: ffbebba84aad52e5 14 BCybDti4zyAi6rL/Bqyo6Gf3pQs (comment: *****The definition of this term is not right) Figure 2 ------- COMMENT: ffbebba84aad52e5 15 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: ffbebba84aad52e5 16 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: ffbebba84aad52e5 17 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 ------- COMMENT: ffbebba84aad52e5 18 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: ffbebba84aad52e5 19 rgMuQlilkJQuV3vloq8KFjK/38c Figure 3 ------- COMMENT: ffbebba84aad52e5 20 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: ffbebba84aad52e5 21 kDWFIcxVoMhBSZ5IahpapH1+ewA Figure 4 ------- COMMENT: ffbebba84aad52e5 22 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: ffbebba84aad52e5 23 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: ffbebba84aad52e5 24 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: ffbebba84aad52e5 25 vrF5EYQw+x9c+LFoPJJXKS6bM9c Figure 5 ------- COMMENT: ffbebba84aad52e5 26 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: ffbebba84aad52e5 27 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: ffbebba84aad52e5 28 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: ffbebba84aad52e5 29 ak7utVasYg8urFMF8vmG+TQUXHE Figure 6 ------- COMMENT: ffbebba84aad52e5 30 dcdtUwg7R1lncRlCtgePm40r2vE Figure 7, 1 ------- COMMENT: ffbebba84aad52e5 31 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: ffbebba84aad52e5 32 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: ffbebba84aad52e5 33 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: ffbebba84aad52e5 34 c6ON6C7HWjUkhly5n2ndFgm/iEw (comment: ***DELAYED) Figure 7 ------- COMMENT: ffbebba84aad52e5 35 qGHqHM21xfTzMcjjVqGVCOROePs (comment: DELAYED) Figure 7 ------- COMMENT: ffbebba84aad52e5 36 imSBqq8JGyTyWfJmFsk589LKN00 Figure 7 ------- COMMENT: ffbebba84aad52e5 37 riH6PuZeW9pd2w953irT9kPPwpE Figure 8 ------- COMMENT: ffbebba84aad52e5 38 riH6PuZeW9pd2w953irT9kPPwpE Figure 8 ------- COMMENT: ffbebba84aad52e5 39 7g18yafIq/fy4Yr72wQtQZMcGG0 Figure 8 (comment: anaphase B) ------- COMMENT: ffbebba84aad52e5 40 elrXqMEoPJJy5+dR1Ki3mGwOccI Figure 2 -------