# alleles

id: SJAG_00028:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"rga6","product":"RhoGAP for Cdc42, Rga6","uniquename":"SJAG_00028"},"genes_by_uniquename":{},"genotypes":[{"display_name":"rga4delta rga6delta","display_uniquename":"rga4delta__rga6delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04522:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_00028:allele-1","expression":"Null"}]}]},{"display_name":"rga6delta","display_uniquename":"rga6delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00028:allele-1","expression":"Null"}]}]},{"display_name":"rga6delta wee1-as8(T582G aa)","display_uniquename":"rga6delta__wee1-as8-T582G-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00028:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_04280:allele-1","expression":"Not assayed or wild type"}]}]}],"name":"rga6delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0001320","FYPO:0000002","FYPO:0001985","FYPO:0005447"],"is_obsolete":false,"name":"abolished geometry scaling","termid":"FYPO:0007783"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":2,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0000002","FYPO:0001320"],"is_obsolete":false,"name":"abolished polar cell growth","termid":"FYPO:0004105"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000005","FYPO:0000002","FYPO:0001985","FYPO:0001320","FYPO:0000004"],"is_obsolete":false,"name":"viable vegetative cell with increased cell diameter","termid":"FYPO:0006616"}],"references_by_uniquename":{},"uniquename":"SJAG_00028:allele-1"}


id: SJAG_00118:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"mid2","product":"medial ring protein, anillin Mid2","uniquename":"SJAG_00118"},"genes_by_uniquename":{},"genotypes":[{"display_name":"mid1delta mid2delta","display_uniquename":"mid1delta__mid2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01143:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_00118:allele-1","expression":"Null"}]}]},{"display_name":"mid2delta","display_uniquename":"mid2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00118:allele-1","expression":"Null"}]}]}],"name":"mid2delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":3,"interesting_parent_ids":["FYPO:0001320","FYPO:0000631","FYPO:0001985","FYPO:0000002","FYPO:0005447","FYPO:0000032"],"is_obsolete":false,"name":"multiseptate vegetative cell","termid":"FYPO:0000118"}],"references_by_uniquename":{},"uniquename":"SJAG_00118:allele-1"}


id: SJAG_00124:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"product":"NRG1-like transcriptional repressor, zinc finger C2H2-type","uniquename":"SJAG_00124"},"genes_by_uniquename":{},"genotypes":[{"display_name":"SJAG_00124delta","display_uniquename":"SJAG_00124delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00124:allele-1","expression":"Null"}]}]},{"display_name":"SJAG_00124delta sty1delta","display_uniquename":"SJAG_00124delta__sty1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00124:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_02592:allele-1","expression":"Null"}]}]}],"name":"SJAG_00124delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":6,"genotype_count":9,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"decreased hyphal length","termid":"FYPO:0007776"},{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":8,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"decreased mycelial expansion","termid":"FYPO:0007780"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"increased mycelial expansion","termid":"FYPO:0007779"}],"references_by_uniquename":{},"uniquename":"SJAG_00124:allele-1"}


id: SJAG_00266:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"atf1","product":"DNA-binding transcription factor, Atf-CREB family Atf1","uniquename":"SJAG_00266"},"genes_by_uniquename":{},"genotypes":[{"display_name":"atf1delta","display_uniquename":"atf1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00266:allele-1","expression":"Null"}]}]}],"name":"atf1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0001985","FYPO:0002060","FYPO:0002057","FYPO:0000139","FYPO:0005447","FYPO:0000003","FYPO:0002058"],"is_obsolete":false,"name":"abolished cell population growth at high temperature","termid":"FYPO:0004481"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0001985","FYPO:0000003"],"is_obsolete":false,"name":"decreased mating efficiency","termid":"FYPO:0000708"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985","FYPO:0005447"],"is_obsolete":false,"name":"decreased quorum sensing response","termid":"FYPO:0007824"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0000005","FYPO:0001985","FYPO:0001320","FYPO:0000002"],"is_obsolete":false,"name":"elongated vegetative cell","termid":"FYPO:0001122"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000002","FYPO:0007773"],"is_obsolete":false,"name":"increased hyphal branching","termid":"FYPO:0007777"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"increased hyphal length","termid":"FYPO:0007775"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"increased invasive hyphal growth","termid":"FYPO:0007774"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0005447","FYPO:0001985","FYPO:0000003","FYPO:0000139","FYPO:0002683"],"is_obsolete":false,"name":"sensitive to caffeine during vegetative growth","termid":"FYPO:0000097"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000003","FYPO:0005447","FYPO:0000139","FYPO:0001985","FYPO:0002683"],"is_obsolete":false,"name":"sensitive to latrunculin A","termid":"FYPO:0000107"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0001985","FYPO:0000003","FYPO:0000139","FYPO:0005447"],"is_obsolete":false,"name":"sensitive to oxidative stress","termid":"FYPO:0000726"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0005447","FYPO:0001985","FYPO:0000139","FYPO:0000003"],"is_obsolete":false,"name":"sensitive to salt stress","termid":"FYPO:0000271"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000003","FYPO:0005447","FYPO:0000139","FYPO:0002683","FYPO:0001985"],"is_obsolete":false,"name":"sensitive to sodium dodecyl sulfate","termid":"FYPO:0000841"}],"references_by_uniquename":{},"uniquename":"SJAG_00266:allele-1"}


id: SJAG_00364:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"rad3","product":"ATR checkpoint kinase Rad3","uniquename":"SJAG_00364"},"genes_by_uniquename":{},"genotypes":[{"display_name":"rad3delta","display_uniquename":"rad3delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00364:allele-1","expression":"Null"}]}]}],"name":"rad3delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":8,"genotype_count":8,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"abolished hyphal transition","termid":"FYPO:0007782"},{"cv_name":"fission_yeast_phenotype","gene_count":7,"genotype_count":8,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0000139","FYPO:0002683","FYPO:0000003"],"is_obsolete":false,"name":"sensitive to camptothecin","termid":"FYPO:0000085"},{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":6,"interesting_parent_ids":["FYPO:0000003","FYPO:0005447","FYPO:0001985","FYPO:0002683","FYPO:0000139"],"is_obsolete":false,"name":"sensitive to hydroxyurea","termid":"FYPO:0000088"},{"cv_name":"fission_yeast_phenotype","gene_count":9,"genotype_count":9,"interesting_parent_ids":["FYPO:0000139","FYPO:0005447","FYPO:0000003","FYPO:0002058","FYPO:0002057","FYPO:0001985","FYPO:0002060"],"is_obsolete":false,"name":"slow vegetative cell population growth","termid":"FYPO:0001234"}],"references_by_uniquename":{},"uniquename":"SJAG_00364:allele-1"}


id: SJAG_00409:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"gpd1","product":"glycerol-3-phosphate dehydrogenase Gpd1","uniquename":"SJAG_00409"},"genes_by_uniquename":{},"genotypes":[{"display_name":"gpd1delta","display_uniquename":"gpd1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00409:allele-1","expression":"Null"}]}]}],"name":"gpd1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0005568","FYPO:0001985","FYPO:0000002","FYPO:0005447","FYPO:0001320"],"is_obsolete":false,"name":"abnormal vegetative cell polarity","termid":"FYPO:0000026"},{"cv_name":"fission_yeast_phenotype","gene_count":13,"genotype_count":15,"interesting_parent_ids":["FYPO:0002058","FYPO:0005447","FYPO:0001985","FYPO:0002060","FYPO:0002057","FYPO:0000139","FYPO:0000003"],"is_obsolete":false,"name":"decreased vegetative cell population growth","termid":"FYPO:0001355"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0000004","FYPO:0001320","FYPO:0000002"],"is_obsolete":false,"name":"inviable vegetative cell","termid":"FYPO:0001489"}],"references_by_uniquename":{},"uniquename":"SJAG_00409:allele-1"}


id: SJAG_00426:allele-1
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"P363S","gene":{"name":"bbl1","product":"ER phosphatidate cytidylyltransferase Bbl1","uniquename":"SJAG_00426"},"genes_by_uniquename":{},"genotypes":[{"display_name":"bbl1-P363S(aa)","display_uniquename":"bbl1-P363S-P363S-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00426:allele-1","expression":"Not assayed or wild type"}]}]}],"name":"bbl1-P363S","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000002","FYPO:0005447","FYPO:0001985","FYPO:0001320"],"is_obsolete":false,"name":"abnormal lipid droplet morphology","termid":"FYPO:0000356"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0001320","FYPO:0000002","FYPO:0005447","FYPO:0001985"],"is_obsolete":false,"name":"abnormal tubular endoplasmic reticulum morphology","termid":"FYPO:0006433"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0001985","FYPO:0001320","FYPO:0000002"],"is_obsolete":false,"name":"increased cellular diglyceride level","termid":"FYPO:0004695"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0001320","FYPO:0001985","FYPO:0000002"],"is_obsolete":false,"name":"increased cellular triglyceride level during vegetative growth","termid":"FYPO:0002227"},{"cv_name":"fission_yeast_phenotype","gene_count":7,"genotype_count":8,"interesting_parent_ids":["FYPO:0000139","FYPO:0000003","FYPO:0000257","FYPO:0002058","FYPO:0002057","FYPO:0002060"],"is_obsolete":false,"name":"normal vegetative cell population growth","termid":"FYPO:0001357"}],"references_by_uniquename":{},"uniquename":"SJAG_00426:allele-1"}


id: SJAG_00429:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"rad26","product":"ATRIP, ATR checkpoint kinase regulatory subunit Rad26","uniquename":"SJAG_00429"},"genes_by_uniquename":{},"genotypes":[{"display_name":"rad26delta","display_uniquename":"rad26delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00429:allele-1","expression":"Null"}]}]}],"name":"rad26delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":8,"genotype_count":8,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"abolished hyphal transition","termid":"FYPO:0007782"},{"cv_name":"fission_yeast_phenotype","gene_count":7,"genotype_count":8,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0000139","FYPO:0002683","FYPO:0000003"],"is_obsolete":false,"name":"sensitive to camptothecin","termid":"FYPO:0000085"},{"cv_name":"fission_yeast_phenotype","gene_count":9,"genotype_count":9,"interesting_parent_ids":["FYPO:0000139","FYPO:0005447","FYPO:0000003","FYPO:0002058","FYPO:0002057","FYPO:0001985","FYPO:0002060"],"is_obsolete":false,"name":"slow vegetative cell population growth","termid":"FYPO:0001234"}],"references_by_uniquename":{},"uniquename":"SJAG_00429:allele-1"}


id: SJAG_00514:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"scd1","product":"RhoGEF Scd1","uniquename":"SJAG_00514"},"genes_by_uniquename":{},"genotypes":[{"display_name":"scd1delta","display_uniquename":"scd1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00514:allele-1","expression":"Null"}]}]}],"name":"scd1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":5,"interesting_parent_ids":["FYPO:0000002","FYPO:0007773","FYPO:0001985","FYPO:0005447"],"is_obsolete":false,"name":"abolished hyphal growth","termid":"FYPO:0007789"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":2,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0000002","FYPO:0001320"],"is_obsolete":false,"name":"abolished polar cell growth","termid":"FYPO:0004105"},{"cv_name":"fission_yeast_phenotype","gene_count":13,"genotype_count":15,"interesting_parent_ids":["FYPO:0002058","FYPO:0005447","FYPO:0001985","FYPO:0002060","FYPO:0002057","FYPO:0000139","FYPO:0000003"],"is_obsolete":false,"name":"decreased vegetative cell population growth","termid":"FYPO:0001355"},{"cv_name":"fission_yeast_phenotype","gene_count":9,"genotype_count":9,"interesting_parent_ids":["FYPO:0000139","FYPO:0005447","FYPO:0000003","FYPO:0002058","FYPO:0002057","FYPO:0001985","FYPO:0002060"],"is_obsolete":false,"name":"slow vegetative cell population growth","termid":"FYPO:0001234"},{"cv_name":"fission_yeast_phenotype","gene_count":10,"genotype_count":10,"interesting_parent_ids":["FYPO:0001985","FYPO:0000301","FYPO:0005447"],"is_obsolete":false,"name":"sterile","secondary_identifiers":["FYPO:0000638"],"termid":"FYPO:0000280"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0001985","FYPO:0000002","FYPO:0000005","FYPO:0001320","FYPO:0000004"],"is_obsolete":false,"name":"viable spheroid vegetative cell","termid":"FYPO:0002380"}],"references_by_uniquename":{},"uniquename":"SJAG_00514:allele-1"}


id: SJAG_00601:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"rip1","product":"Rieske iron-sulfur protein, ubiquinol-cytochrome-c reductase complex subunit 5, Rip1","uniquename":"SJAG_00601"},"genes_by_uniquename":{},"genotypes":[{"display_name":"rip1delta","display_uniquename":"rip1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00601:allele-1","expression":"Null"}]}]}],"name":"rip1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":7,"genotype_count":6,"interesting_parent_ids":["FYPO:0000257"],"is_obsolete":false,"name":"normal hyphal growth","termid":"FYPO:0007790"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000301","FYPO:0000257"],"is_obsolete":false,"name":"normal mating","termid":"FYPO:0000760"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000002","FYPO:0000257"],"is_obsolete":false,"name":"normal spore germination","termid":"FYPO:0000579"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000002","FYPO:0000257"],"is_obsolete":false,"name":"normal sporulation","termid":"FYPO:0000590"},{"cv_name":"fission_yeast_phenotype","gene_count":7,"genotype_count":8,"interesting_parent_ids":["FYPO:0000139","FYPO:0000003","FYPO:0000257","FYPO:0002058","FYPO:0002057","FYPO:0002060"],"is_obsolete":false,"name":"normal vegetative cell population growth","termid":"FYPO:0001357"}],"references_by_uniquename":{},"uniquename":"SJAG_00601:allele-1"}


id: SJAG_00610:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"pck2","product":"protein kinase C (PKC)-like Pck2","uniquename":"SJAG_00610"},"genes_by_uniquename":{},"genotypes":[{"display_name":"pck2delta","display_uniquename":"pck2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00610:allele-1","expression":"Null"}]}]}],"name":"pck2delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0002494","FYPO:0005447","FYPO:0002274","FYPO:0001985","FYPO:0000002"],"is_obsolete":false,"name":"abolished protein phosphorylation","termid":"FYPO:0002678"},{"cv_name":"fission_yeast_phenotype","gene_count":6,"genotype_count":9,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"decreased hyphal length","termid":"FYPO:0007776"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0005447","FYPO:0000002","FYPO:0002274","FYPO:0001320","FYPO:0001985","FYPO:0002494"],"is_obsolete":false,"name":"decreased protein phosphorylation during vegetative growth","termid":"FYPO:0001838"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":5,"interesting_parent_ids":["FYPO:0000139","FYPO:0005447","FYPO:0000003","FYPO:0001985","FYPO:0002682"],"is_obsolete":false,"name":"resistance to tacrolimus and magnesium chloride","termid":"FYPO:0007521"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000003","FYPO:0000139","FYPO:0002683","FYPO:0005447","FYPO:0001985"],"is_obsolete":false,"name":"sensitive to caspofungin","termid":"FYPO:0000079"}],"references_by_uniquename":{},"uniquename":"SJAG_00610:allele-1"}


id: SJAG_00871:allele-1
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"cut3-T19E","gene":{"name":"cut3","product":"condensin complex SMC subunit Smc4","uniquename":"SJAG_00871"},"genes_by_uniquename":{},"genotypes":[{"display_name":"cut3-T19E(aa) pim1-R152C(aa)","display_uniquename":"cut3-T19E-cut3-T19E-amino_acid_mutation-expression-not_assayed_or_wild_type__pim1-R152C-R152C-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00871:allele-1","expression":"Not assayed or wild type"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_04464:allele-1","expression":"Not assayed or wild type"}]}]}],"name":"cut3-T19E","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":2,"interesting_parent_ids":["FYPO:0000002","FYPO:0000631","FYPO:0001985","FYPO:0005447","FYPO:0001320"],"is_obsolete":false,"name":"abnormal cell cycle arrest in mitotic anaphase","termid":"FYPO:0000619"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0000004","FYPO:0001320","FYPO:0000002"],"is_obsolete":false,"name":"inviable vegetative cell","termid":"FYPO:0001489"}],"references_by_uniquename":{},"uniquename":"SJAG_00871:allele-1"}


id: SJAG_00993:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"byr1","product":"MAP kinase kinase Byr1","uniquename":"SJAG_00993"},"genes_by_uniquename":{},"genotypes":[{"display_name":"byr1delta","display_uniquename":"byr1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00993:allele-1","expression":"Null"}]}]}],"name":"byr1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":10,"genotype_count":10,"interesting_parent_ids":["FYPO:0001985","FYPO:0000301","FYPO:0005447"],"is_obsolete":false,"name":"sterile","secondary_identifiers":["FYPO:0000638"],"termid":"FYPO:0000280"},{"cv_name":"fission_yeast_phenotype","gene_count":23,"genotype_count":24,"interesting_parent_ids":["FYPO:0000002","FYPO:0000004"],"is_obsolete":false,"name":"viable cell","termid":"FYPO:0000124"}],"references_by_uniquename":{},"uniquename":"SJAG_00993:allele-1"}


id: SJAG_01050:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"nur1","product":"Lem2-Nur1 complex subunit Nur1","uniquename":"SJAG_01050"},"genes_by_uniquename":{},"genotypes":[{"display_name":"nur1delta","display_uniquename":"nur1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01050:allele-1","expression":"Null"}]}]},{"display_name":"nur1delta vps4delta","display_uniquename":"nur1delta__vps4delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01050:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_03892:allele-1","expression":"Null"}]}]}],"name":"nur1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":1,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985","FYPO:0001320","FYPO:0000138","FYPO:0005447"],"is_obsolete":false,"name":"abolished protein localization to heterochromatin during vegetative growth","termid":"FYPO:0001132"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0001320","FYPO:0000002","FYPO:0000138"],"is_obsolete":false,"name":"decreased protein localization to nuclear envelope during vegetative growth","termid":"FYPO:0007073"}],"references_by_uniquename":{},"uniquename":"SJAG_01050:allele-1"}


id: SJAG_01081:allele-1
data:
  {"allele_type":"nonsense_mutation","alleles_by_uniquename":{},"description":"S427>stop","gene":{"name":"ade6","product":"phosphoribosylaminoimidazole carboxylase Ade6","uniquename":"SJAG_01081"},"genes_by_uniquename":{},"genotypes":[{"display_name":"ade6-domE(S427>stop)","display_uniquename":"ade6-domE-S427>stop-nonsense_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01081:allele-1","expression":"Not assayed or wild type"}]}]}],"name":"ade6-domE","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":3,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985"],"is_obsolete":false,"name":"growth auxotrophic for adenine","termid":"FYPO:0000747"}],"references_by_uniquename":{},"uniquename":"SJAG_01081:allele-1"}


id: SJAG_01081:allele-2
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"Q262A","gene":{"name":"ade6","product":"phosphoribosylaminoimidazole carboxylase Ade6","uniquename":"SJAG_01081"},"genes_by_uniquename":{},"genotypes":[{"display_name":"ade6-domK(Q262A aa)","display_uniquename":"ade6-domK-Q262A-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01081:allele-2","expression":"Not assayed or wild type"}]}]}],"name":"ade6-domK","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":3,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985"],"is_obsolete":false,"name":"growth auxotrophic for adenine","termid":"FYPO:0000747"}],"references_by_uniquename":{},"uniquename":"SJAG_01081:allele-2"}


id: SJAG_01081:allele-3
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"ade6","product":"phosphoribosylaminoimidazole carboxylase Ade6","uniquename":"SJAG_01081"},"genes_by_uniquename":{},"genotypes":[{"display_name":"ade6delta","display_uniquename":"ade6delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01081:allele-3","expression":"Null"}]}]}],"name":"ade6delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":3,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985"],"is_obsolete":false,"name":"growth auxotrophic for adenine","termid":"FYPO:0000747"}],"references_by_uniquename":{},"uniquename":"SJAG_01081:allele-3"}


id: SJAG_01113:allele-1
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"R47K","gene":{"product":"mating-type transcription factor (Pc-like)","uniquename":"SJAG_01113"},"genes_by_uniquename":{},"genotypes":[],"name":"mat-Pc-R47K","phenotypes":[],"references_by_uniquename":{},"uniquename":"SJAG_01113:allele-1"}


id: SJAG_01143:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"mid1","product":"anillin-related medial ring protein Mid1","uniquename":"SJAG_01143"},"genes_by_uniquename":{},"genotypes":[{"display_name":"cdc15-A(L264P,P442T aa) mid1delta","display_uniquename":"cdc15-A-L264P,P442T-amino_acid_mutation-expression-not_assayed_or_wild_type__mid1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03596:allele-1","expression":"Not assayed or wild type"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_01143:allele-1","expression":"Null"}]}]},{"display_name":"mid1delta","display_uniquename":"mid1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01143:allele-1","expression":"Null"}]}]},{"display_name":"mid1delta mid2delta","display_uniquename":"mid1delta__mid2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01143:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_00118:allele-1","expression":"Null"}]}]},{"display_name":"mid1delta pom1delta","display_uniquename":"mid1delta__pom1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01143:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_02392:allele-1","expression":"Null"}]}]}],"name":"mid1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0001985","FYPO:0000032","FYPO:0000002","FYPO:0005447","FYPO:0004803","FYPO:0001320","FYPO:0000631"],"is_obsolete":false,"name":"abnormal actomyosin contractile ring myosin filament organization","termid":"FYPO:0000231"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0000138","FYPO:0005447","FYPO:0001985","FYPO:0000002","FYPO:0001320"],"is_obsolete":false,"name":"abnormally monopolar protein localization to cell tip","termid":"FYPO:0005503"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0005447","FYPO:0001320","FYPO:0001985","FYPO:0000138","FYPO:0000002"],"is_obsolete":false,"name":"abolished protein localization to medial cortex during mitotic interphase","termid":"FYPO:0007177"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000002","FYPO:0000631","FYPO:0001320","FYPO:0005447","FYPO:0000032","FYPO:0001985","FYPO:0004803"],"is_obsolete":false,"name":"delayed onset of actomyosin contractile ring assembly","termid":"FYPO:0000729"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":1,"interesting_parent_ids":["FYPO:0000138","FYPO:0000002","FYPO:0001320","FYPO:0005447","FYPO:0001985"],"is_obsolete":false,"name":"delayed onset of protein localization to cell division site","termid":"FYPO:0003946"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":3,"interesting_parent_ids":["FYPO:0001320","FYPO:0000631","FYPO:0001985","FYPO:0000002","FYPO:0005447","FYPO:0000032"],"is_obsolete":false,"name":"multiseptate vegetative cell","termid":"FYPO:0000118"}],"references_by_uniquename":{},"uniquename":"SJAG_01143:allele-1"}


id: SJAG_01143:allele-2
data:
  {"allele_type":"partial_amino_acid_deletion","alleles_by_uniquename":{},"description":"711-723","gene":{"name":"mid1","product":"anillin-related medial ring protein Mid1","uniquename":"SJAG_01143"},"genes_by_uniquename":{},"genotypes":[{"display_name":"mid1-delta(711-723 a.a.)(aa)","display_uniquename":"mid1-delta(711-723_a.a.)-711-723-partial_amino_acid_deletion-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01143:allele-2","expression":"Not assayed or wild type"}]}]}],"name":"mid1-delta(711-723 a.a.)","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000257","FYPO:0000138","FYPO:0001312","FYPO:0001320","FYPO:0000002"],"is_obsolete":false,"name":"normal protein localization during vegetative growth","termid":"FYPO:0000644"}],"references_by_uniquename":{},"uniquename":"SJAG_01143:allele-2"}


id: SJAG_01181:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"pek1","product":"MAP kinase kinase Pek1","uniquename":"SJAG_01181"},"genes_by_uniquename":{},"genotypes":[{"display_name":"pek1delta","display_uniquename":"pek1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01181:allele-1","expression":"Null"}]}]}],"name":"pek1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":23,"genotype_count":24,"interesting_parent_ids":["FYPO:0000002","FYPO:0000004"],"is_obsolete":false,"name":"viable cell","termid":"FYPO:0000124"}],"references_by_uniquename":{},"uniquename":"SJAG_01181:allele-1"}


id: SJAG_01205:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"crb2","product":"BRCT and tudor domain DNA checkpoint protein Crb2","uniquename":"SJAG_01205"},"genes_by_uniquename":{},"genotypes":[{"display_name":"chk1delta crb2delta","display_uniquename":"chk1delta__crb2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01680:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_01205:allele-1","expression":"Null"}]}]},{"display_name":"crb2delta","display_uniquename":"crb2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01205:allele-1","expression":"Null"}]}]}],"name":"crb2delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":8,"genotype_count":8,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"abolished hyphal transition","termid":"FYPO:0007782"},{"cv_name":"fission_yeast_phenotype","gene_count":7,"genotype_count":8,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0000139","FYPO:0002683","FYPO:0000003"],"is_obsolete":false,"name":"sensitive to camptothecin","termid":"FYPO:0000085"},{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":6,"interesting_parent_ids":["FYPO:0000003","FYPO:0005447","FYPO:0001985","FYPO:0002683","FYPO:0000139"],"is_obsolete":false,"name":"sensitive to hydroxyurea","termid":"FYPO:0000088"}],"references_by_uniquename":{},"uniquename":"SJAG_01205:allele-1"}


id: SJAG_01242:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"vps32","product":"ESCRT III complex subunit Vps32","uniquename":"SJAG_01242"},"genes_by_uniquename":{},"genotypes":[{"display_name":"vps32delta","display_uniquename":"vps32delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01242:allele-1","expression":"Null"}]}]}],"name":"vps32delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":5,"interesting_parent_ids":["FYPO:0001985","FYPO:0000002","FYPO:0001320","FYPO:0005447"],"is_obsolete":false,"name":"delayed onset of mitotic nuclear membrane reassembly","termid":"FYPO:0007772"},{"cv_name":"fission_yeast_phenotype","gene_count":9,"genotype_count":9,"interesting_parent_ids":["FYPO:0000139","FYPO:0005447","FYPO:0000003","FYPO:0002058","FYPO:0002057","FYPO:0001985","FYPO:0002060"],"is_obsolete":false,"name":"slow vegetative cell population growth","termid":"FYPO:0001234"}],"references_by_uniquename":{},"uniquename":"SJAG_01242:allele-1"}


id: SJAG_01330:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"mad2","product":"mitotic spindle checkpoint protein Mad2","uniquename":"SJAG_01330"},"genes_by_uniquename":{},"genotypes":[{"display_name":"mad2delta","display_uniquename":"mad2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01330:allele-1","expression":"Null"}]}]}],"name":"mad2delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000002","FYPO:0005447","FYPO:0001320","FYPO:0000631","FYPO:0001985","FYPO:0000298"],"is_obsolete":false,"name":"abolished mitotic spindle assembly checkpoint","termid":"FYPO:0004318"}],"references_by_uniquename":{},"uniquename":"SJAG_01330:allele-1"}


id: SJAG_01355:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"cox6","product":"cytochrome c oxidase subunit VI","uniquename":"SJAG_01355"},"genes_by_uniquename":{},"genotypes":[{"display_name":"cox6delta","display_uniquename":"cox6delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01355:allele-1","expression":"Null"}]}]}],"name":"cox6delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":7,"genotype_count":6,"interesting_parent_ids":["FYPO:0000257"],"is_obsolete":false,"name":"normal hyphal growth","termid":"FYPO:0007790"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000301","FYPO:0000257"],"is_obsolete":false,"name":"normal mating","termid":"FYPO:0000760"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000002","FYPO:0000257"],"is_obsolete":false,"name":"normal spore germination","termid":"FYPO:0000579"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000002","FYPO:0000257"],"is_obsolete":false,"name":"normal sporulation","termid":"FYPO:0000590"},{"cv_name":"fission_yeast_phenotype","gene_count":7,"genotype_count":8,"interesting_parent_ids":["FYPO:0000139","FYPO:0000003","FYPO:0000257","FYPO:0002058","FYPO:0002057","FYPO:0002060"],"is_obsolete":false,"name":"normal vegetative cell population growth","termid":"FYPO:0001357"},{"cv_name":"fission_yeast_phenotype","gene_count":9,"genotype_count":9,"interesting_parent_ids":["FYPO:0000139","FYPO:0005447","FYPO:0000003","FYPO:0002058","FYPO:0002057","FYPO:0001985","FYPO:0002060"],"is_obsolete":false,"name":"slow vegetative cell population growth","termid":"FYPO:0001234"}],"references_by_uniquename":{},"uniquename":"SJAG_01355:allele-1"}


id: SJAG_01374:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"nup132","product":"nucleoporin, WD repeat Nup132","uniquename":"SJAG_01374"},"genes_by_uniquename":{},"genotypes":[{"display_name":"nup132delta","display_uniquename":"nup132delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01374:allele-1","expression":"Null"}]}]}],"name":"nup132delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985","FYPO:0005447","FYPO:0001320"],"is_obsolete":false,"name":"abnormal nuclear pore localization","termid":"FYPO:0003973"}],"references_by_uniquename":{},"uniquename":"SJAG_01374:allele-1"}


id: SJAG_01410:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"rad51","product":"RecA family recombinase Rad51/Rhp51","uniquename":"SJAG_01410"},"genes_by_uniquename":{},"genotypes":[{"display_name":"rad51delta","display_uniquename":"rad51delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01410:allele-1","expression":"Null"}]}]}],"name":"rad51delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":7,"genotype_count":8,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0000139","FYPO:0002683","FYPO:0000003"],"is_obsolete":false,"name":"sensitive to camptothecin","termid":"FYPO:0000085"}],"references_by_uniquename":{},"uniquename":"SJAG_01410:allele-1"}


id: SJAG_01458:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"acb1","product":"fatty-acyl-CoA binding protein Acb1","uniquename":"SJAG_01458"},"genes_by_uniquename":{},"genotypes":[{"display_name":"acb1delta","display_uniquename":"acb1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01458:allele-1","expression":"Null"}]}]}],"name":"acb1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000654","FYPO:0000257","FYPO:0000652"],"is_obsolete":false,"name":"normal fatty acid synthase activity","termid":"FYPO:0000719"}],"references_by_uniquename":{},"uniquename":"SJAG_01458:allele-1"}


id: SJAG_01539:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"nde1","product":"external mitochondrial NADH dehydrogenase (ubiquinone) Nde1/Nde2","uniquename":"SJAG_01539"},"genes_by_uniquename":{},"genotypes":[],"name":"nde1delta","phenotypes":[],"references_by_uniquename":{},"uniquename":"SJAG_01539:allele-1"}


id: SJAG_01605:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"rad9","product":"checkpoint clamp complex protein Rad9","uniquename":"SJAG_01605"},"genes_by_uniquename":{},"genotypes":[{"display_name":"rad9delta","display_uniquename":"rad9delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01605:allele-1","expression":"Null"}]}]}],"name":"rad9delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":8,"genotype_count":8,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"abolished hyphal transition","termid":"FYPO:0007782"},{"cv_name":"fission_yeast_phenotype","gene_count":7,"genotype_count":8,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0000139","FYPO:0002683","FYPO:0000003"],"is_obsolete":false,"name":"sensitive to camptothecin","termid":"FYPO:0000085"}],"references_by_uniquename":{},"uniquename":"SJAG_01605:allele-1"}


id: SJAG_01680:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"chk1","product":"Chk1 protein kinase","uniquename":"SJAG_01680"},"genes_by_uniquename":{},"genotypes":[{"display_name":"chk1delta","display_uniquename":"chk1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01680:allele-1","expression":"Null"}]}]},{"display_name":"chk1delta crb2delta","display_uniquename":"chk1delta__crb2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01680:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_01205:allele-1","expression":"Null"}]}]}],"name":"chk1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":8,"genotype_count":8,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"abolished hyphal transition","termid":"FYPO:0007782"},{"cv_name":"fission_yeast_phenotype","gene_count":7,"genotype_count":8,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0000139","FYPO:0002683","FYPO:0000003"],"is_obsolete":false,"name":"sensitive to camptothecin","termid":"FYPO:0000085"},{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":6,"interesting_parent_ids":["FYPO:0000003","FYPO:0005447","FYPO:0001985","FYPO:0002683","FYPO:0000139"],"is_obsolete":false,"name":"sensitive to hydroxyurea","termid":"FYPO:0000088"}],"references_by_uniquename":{},"uniquename":"SJAG_01680:allele-1"}


id: SJAG_01680:allele-2
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"P470L","gene":{"name":"chk1","product":"Chk1 protein kinase","uniquename":"SJAG_01680"},"genes_by_uniquename":{},"genotypes":[{"display_name":"chk1-P470L(aa)","display_uniquename":"chk1-P470L-P470L-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01680:allele-2","expression":"Not assayed or wild type"}]}]},{"display_name":"chk1-P470L(aa) rad24delta","display_uniquename":"chk1-P470L-P470L-amino_acid_mutation-expression-not_assayed_or_wild_type__rad24delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01680:allele-2","expression":"Not assayed or wild type"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_02266:allele-1","expression":"Null"}]}]}],"name":"chk1-P470L","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"increased invasive hyphal growth","termid":"FYPO:0007774"},{"cv_name":"fission_yeast_phenotype","gene_count":7,"genotype_count":6,"interesting_parent_ids":["FYPO:0000257"],"is_obsolete":false,"name":"normal hyphal growth","termid":"FYPO:0007790"}],"references_by_uniquename":{},"synonyms":[{"name":"chk1- hyperactive (chk1-hyp)","reference":"PMID:23090706","type":"exact"},{"name":"chk1-hyperactive (chk1-hyp)","reference":"PMID:20368354","type":"exact"}],"uniquename":"SJAG_01680:allele-2"}


id: SJAG_01718:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"byr2","product":"MAP kinase kinase kinase Byr2","uniquename":"SJAG_01718"},"genes_by_uniquename":{},"genotypes":[{"display_name":"byr2delta","display_uniquename":"byr2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01718:allele-1","expression":"Null"}]}]}],"name":"byr2delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":10,"genotype_count":10,"interesting_parent_ids":["FYPO:0001985","FYPO:0000301","FYPO:0005447"],"is_obsolete":false,"name":"sterile","secondary_identifiers":["FYPO:0000638"],"termid":"FYPO:0000280"},{"cv_name":"fission_yeast_phenotype","gene_count":23,"genotype_count":24,"interesting_parent_ids":["FYPO:0000002","FYPO:0000004"],"is_obsolete":false,"name":"viable cell","termid":"FYPO:0000124"}],"references_by_uniquename":{},"uniquename":"SJAG_01718:allele-1"}


id: SJAG_01745:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"lem2","product":"LEM domain nuclear inner membrane protein Heh1/Lem2","uniquename":"SJAG_01745"},"genes_by_uniquename":{},"genotypes":[{"display_name":"lem2delta","display_uniquename":"lem2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01745:allele-1","expression":"Null"}]}]}],"name":"lem2delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":4,"interesting_parent_ids":["FYPO:0005447","FYPO:0001985","FYPO:0001320","FYPO:0000002","FYPO:0002403"],"is_obsolete":false,"name":"abnormal nuclear envelope morphology during mitosis","termid":"FYPO:0003779"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0000002","FYPO:0000138","FYPO:0001985","FYPO:0005447"],"is_obsolete":false,"name":"abolished protein localization","termid":"FYPO:0001375"},{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":5,"interesting_parent_ids":["FYPO:0001985","FYPO:0000002","FYPO:0001320","FYPO:0005447"],"is_obsolete":false,"name":"delayed onset of mitotic nuclear membrane reassembly","termid":"FYPO:0007772"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0001320","FYPO:0000002","FYPO:0001985","FYPO:0000138","FYPO:0005447"],"is_obsolete":false,"name":"protein mislocalized to endoplasmic reticulum during vegetative growth","termid":"FYPO:0003657"}],"references_by_uniquename":{},"uniquename":"SJAG_01745:allele-1"}


id: SJAG_01753:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"mdh1","product":"malate dehydrogenase Mdh1","uniquename":"SJAG_01753"},"genes_by_uniquename":{},"genotypes":[{"display_name":"mdh1delta","display_uniquename":"mdh1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01753:allele-1","expression":"Null"}]}]}],"name":"mdh1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":31,"genotype_count":36,"interesting_parent_ids":["FYPO:0000003","FYPO:0002057"],"is_obsolete":false,"name":"viable cell population","termid":"FYPO:0002058"}],"references_by_uniquename":{},"uniquename":"SJAG_01753:allele-1"}


id: SJAG_01787:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"pck1","product":"protein kinase C (PKC)-like Pck1","uniquename":"SJAG_01787"},"genes_by_uniquename":{},"genotypes":[{"display_name":"pck1delta","display_uniquename":"pck1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01787:allele-1","expression":"Null"}]}]},{"display_name":"pck1delta pmk1delta","display_uniquename":"pck1delta__pmk1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01787:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_04551:allele-1","expression":"Null"}]}]}],"name":"pck1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":8,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"decreased mycelial expansion","termid":"FYPO:0007780"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"increased hyphal length","termid":"FYPO:0007775"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"increased mycelial expansion","termid":"FYPO:0007779"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0001985","FYPO:0002494","FYPO:0001320","FYPO:0002274","FYPO:0005447","FYPO:0000002"],"is_obsolete":false,"name":"increased protein phosphorylation during vegetative growth","termid":"FYPO:0001038"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0001985","FYPO:0000003","FYPO:0005447","FYPO:0002682","FYPO:0000139"],"is_obsolete":false,"name":"resistance to caspofungin","termid":"FYPO:0005152"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000003","FYPO:0002683","FYPO:0000139","FYPO:0005447","FYPO:0001985"],"is_obsolete":false,"name":"sensitive to tacrolimus and magnesium chloride","termid":"FYPO:0007522"}],"references_by_uniquename":{},"uniquename":"SJAG_01787:allele-1"}


id: SJAG_01794:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"scd2","product":"Cdc42 GTPase complex scaffold subunit Scd2","uniquename":"SJAG_01794"},"genes_by_uniquename":{},"genotypes":[{"display_name":"scd2delta","display_uniquename":"scd2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01794:allele-1","expression":"Null"}]}]}],"name":"scd2delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":5,"interesting_parent_ids":["FYPO:0000002","FYPO:0007773","FYPO:0001985","FYPO:0005447"],"is_obsolete":false,"name":"abolished hyphal growth","termid":"FYPO:0007789"},{"cv_name":"fission_yeast_phenotype","gene_count":13,"genotype_count":15,"interesting_parent_ids":["FYPO:0002058","FYPO:0005447","FYPO:0001985","FYPO:0002060","FYPO:0002057","FYPO:0000139","FYPO:0000003"],"is_obsolete":false,"name":"decreased vegetative cell population growth","termid":"FYPO:0001355"},{"cv_name":"fission_yeast_phenotype","gene_count":10,"genotype_count":10,"interesting_parent_ids":["FYPO:0001985","FYPO:0000301","FYPO:0005447"],"is_obsolete":false,"name":"sterile","secondary_identifiers":["FYPO:0000638"],"termid":"FYPO:0000280"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0001985","FYPO:0000002","FYPO:0000005","FYPO:0001320","FYPO:0000004"],"is_obsolete":false,"name":"viable spheroid vegetative cell","termid":"FYPO:0002380"}],"references_by_uniquename":{},"uniquename":"SJAG_01794:allele-1"}


id: SJAG_01834:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"pom152","product":"nucleoporin, transmembrane ring glycoprotein subunit Pom152","uniquename":"SJAG_01834"},"genes_by_uniquename":{},"genotypes":[{"display_name":"pom152delta","display_uniquename":"pom152delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01834:allele-1","expression":"Null"}]}]}],"name":"pom152delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":4,"interesting_parent_ids":["FYPO:0001985","FYPO:0000138","FYPO:0001320","FYPO:0000002","FYPO:0005447"],"is_obsolete":false,"name":"increased telomere-nuclear envelope distance during mitosis","termid":"FYPO:0004890"}],"references_by_uniquename":{},"uniquename":"SJAG_01834:allele-1"}


id: SJAG_01906:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"nem1","product":"serine/threonine protein phosphatase (Nem1-Spo7 complex) catalytic subunit Nem1","uniquename":"SJAG_01906"},"genes_by_uniquename":{},"genotypes":[{"display_name":"nem1delta","display_uniquename":"nem1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01906:allele-1","expression":"Null"}]}]}],"name":"nem1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":5,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985","FYPO:0005447","FYPO:0001320"],"is_obsolete":false,"name":"excess endoplasmic reticulum membrane present","termid":"FYPO:0001784"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":6,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0000002","FYPO:0002403","FYPO:0001320"],"is_obsolete":false,"name":"excess nuclear envelope present","termid":"FYPO:0001556"}],"references_by_uniquename":{},"uniquename":"SJAG_01906:allele-1"}


id: SJAG_01946:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"cem1","product":"3-oxoacyl-[acyl-carrier-protein]-synthase condensing enzyme Cem1","uniquename":"SJAG_01946"},"genes_by_uniquename":{},"genotypes":[{"display_name":"apc2-361(E548K ts) cem1delta","display_uniquename":"apc2-361-E548K_ts-amino_acid_mutation-expression-not_assayed_or_wild_type__cem1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03575:allele-1","expression":"Not assayed or wild type"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_01946:allele-1","expression":"Null"}]}]},{"display_name":"cem1delta","display_uniquename":"cem1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01946:allele-1","expression":"Null"}]}]}],"name":"cem1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":5,"interesting_parent_ids":["FYPO:0000139","FYPO:0002060","FYPO:0002057","FYPO:0001985","FYPO:0002058","FYPO:0005447","FYPO:0000003"],"is_obsolete":false,"name":"decreased cell population growth at low temperature","secondary_identifiers":["FYPO:0002175"],"termid":"FYPO:0000080"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":4,"interesting_parent_ids":["FYPO:0002058","FYPO:0002057","FYPO:0000003","FYPO:0000139","FYPO:0000257","FYPO:0002060"],"is_obsolete":false,"name":"normal cell population growth at high temperature","termid":"FYPO:0000674"}],"references_by_uniquename":{},"uniquename":"SJAG_01946:allele-1"}


id: SJAG_01956:allele-1
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"E405K ts","gene":{"name":"nuc2","product":"anaphase-promoting complex TPR lobe subcomplex subunit Apc3","uniquename":"SJAG_01956"},"genes_by_uniquename":{},"genotypes":[{"display_name":"nuc2-1051(E405K ts)","display_uniquename":"nuc2-1051-E405K_ts-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01956:allele-1","expression":"Not assayed or wild type"}]}]}],"name":"nuc2-1051","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0000631","FYPO:0005447","FYPO:0000002","FYPO:0001320","FYPO:0001985"],"is_obsolete":false,"name":"increased duration of mitotic anaphase","secondary_identifiers":["FYPO:0000617"],"termid":"FYPO:0000618"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0001985","FYPO:0001320","FYPO:0000002","FYPO:0005447","FYPO:0006551"],"is_obsolete":false,"name":"increased protein level during vegetative growth","secondary_identifiers":["FYPO:0000762"],"termid":"FYPO:0001327"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0002057","FYPO:0001985","FYPO:0000003"],"is_obsolete":false,"name":"loss of viability at high temperature","termid":"FYPO:0001387"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0001985","FYPO:0000631","FYPO:0005447","FYPO:0000002","FYPO:0001320"],"is_obsolete":false,"name":"mitotic metaphase/anaphase transition delay","secondary_identifiers":["FYPO:0000393","FYPO:0004309"],"termid":"FYPO:0000324"}],"references_by_uniquename":{},"uniquename":"SJAG_01956:allele-1"}


id: SJAG_02007:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"etr1","product":"enoyl-[acyl-carrier protein] reductase","uniquename":"SJAG_02007"},"genes_by_uniquename":{},"genotypes":[],"name":"etr1delta","phenotypes":[],"references_by_uniquename":{},"uniquename":"SJAG_02007:allele-1"}


id: SJAG_02013:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"pyp1","product":"protein tyrosine phosphatase Pyp1","uniquename":"SJAG_02013"},"genes_by_uniquename":{},"genotypes":[{"display_name":"pyp1delta","display_uniquename":"pyp1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02013:allele-1","expression":"Null"}]}]}],"name":"pyp1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000002","FYPO:0007773"],"is_obsolete":false,"name":"decreased hyphal branching","termid":"FYPO:0007778"},{"cv_name":"fission_yeast_phenotype","gene_count":6,"genotype_count":9,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"decreased hyphal length","termid":"FYPO:0007776"},{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":8,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"decreased mycelial expansion","termid":"FYPO:0007780"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":4,"interesting_parent_ids":["FYPO:0001985","FYPO:0000004","FYPO:0000002","FYPO:0000005","FYPO:0001320"],"is_obsolete":false,"name":"viable small vegetative cell","termid":"FYPO:0000648"}],"references_by_uniquename":{},"uniquename":"SJAG_02013:allele-1"}


id: SJAG_02252:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"ace2","product":"DNA-binding transcription factor Ace2","uniquename":"SJAG_02252"},"genes_by_uniquename":{},"genotypes":[{"display_name":"ace2delta","display_uniquename":"ace2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02252:allele-1","expression":"Null"}]}]}],"name":"ace2delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0001985","FYPO:0000002","FYPO:0001320","FYPO:0005447"],"is_obsolete":false,"name":"abolished cell separation after cytokinesis","termid":"FYPO:0006557"}],"references_by_uniquename":{},"uniquename":"SJAG_02252:allele-1"}


id: SJAG_02266:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"rad24","product":"14-3-3 protein Rad24","uniquename":"SJAG_02266"},"genes_by_uniquename":{},"genotypes":[{"display_name":"chk1-P470L(aa) rad24delta","display_uniquename":"chk1-P470L-P470L-amino_acid_mutation-expression-not_assayed_or_wild_type__rad24delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01680:allele-2","expression":"Not assayed or wild type"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_02266:allele-1","expression":"Null"}]}]},{"display_name":"rad24delta","display_uniquename":"rad24delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02266:allele-1","expression":"Null"}]}]}],"name":"rad24delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":8,"genotype_count":8,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"abolished hyphal transition","termid":"FYPO:0007782"},{"cv_name":"fission_yeast_phenotype","gene_count":7,"genotype_count":6,"interesting_parent_ids":["FYPO:0000257"],"is_obsolete":false,"name":"normal hyphal growth","termid":"FYPO:0007790"}],"references_by_uniquename":{},"uniquename":"SJAG_02266:allele-1"}


id: SJAG_02342:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"wis1","product":"MAP kinase kinase Wis1","uniquename":"SJAG_02342"},"genes_by_uniquename":{},"genotypes":[{"display_name":"wis1delta","display_uniquename":"wis1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02342:allele-1","expression":"Null"}]}]}],"name":"wis1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":23,"genotype_count":24,"interesting_parent_ids":["FYPO:0000002","FYPO:0000004"],"is_obsolete":false,"name":"viable cell","termid":"FYPO:0000124"}],"references_by_uniquename":{},"uniquename":"SJAG_02342:allele-1"}


id: SJAG_02379:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"gap1","product":"RasGAP Gap1","uniquename":"SJAG_02379"},"genes_by_uniquename":{},"genotypes":[{"display_name":"gap1delta","display_uniquename":"gap1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02379:allele-1","expression":"Null"}]}]}],"name":"gap1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":5,"interesting_parent_ids":["FYPO:0000002","FYPO:0007773","FYPO:0001985","FYPO:0005447"],"is_obsolete":false,"name":"abolished hyphal growth","termid":"FYPO:0007789"},{"cv_name":"fission_yeast_phenotype","gene_count":10,"genotype_count":10,"interesting_parent_ids":["FYPO:0001985","FYPO:0000301","FYPO:0005447"],"is_obsolete":false,"name":"sterile","secondary_identifiers":["FYPO:0000638"],"termid":"FYPO:0000280"},{"cv_name":"fission_yeast_phenotype","gene_count":23,"genotype_count":24,"interesting_parent_ids":["FYPO:0000002","FYPO:0000004"],"is_obsolete":false,"name":"viable cell","termid":"FYPO:0000124"}],"references_by_uniquename":{},"uniquename":"SJAG_02379:allele-1"}


id: SJAG_02392:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"pom1","product":"DYRK family cell polarity protein kinase Pom1","uniquename":"SJAG_02392"},"genes_by_uniquename":{},"genotypes":[{"display_name":"mid1delta pom1delta","display_uniquename":"mid1delta__pom1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01143:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_02392:allele-1","expression":"Null"}]}]},{"display_name":"pom1delta","display_uniquename":"pom1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02392:allele-1","expression":"Null"}]}]}],"name":"pom1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":3,"interesting_parent_ids":["FYPO:0005447","FYPO:0001985","FYPO:0001320","FYPO:0000138","FYPO:0000002"],"is_obsolete":false,"name":"abnormal protein localization to medial cortex during vegetative growth","termid":"FYPO:0000731"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0000138","FYPO:0005447","FYPO:0001985","FYPO:0000002","FYPO:0001320"],"is_obsolete":false,"name":"abnormally monopolar protein localization to cell tip","termid":"FYPO:0005503"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0005447","FYPO:0001320","FYPO:0001985","FYPO:0000138","FYPO:0000002"],"is_obsolete":false,"name":"abolished protein localization to medial cortex during mitotic interphase","termid":"FYPO:0007177"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000002","FYPO:0005447","FYPO:0004803","FYPO:0000631","FYPO:0000138","FYPO:0000032","FYPO:0001320","FYPO:0001985"],"is_obsolete":false,"name":"actomyosin contractile ring displaced from midpoint","termid":"FYPO:0002026"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0001985","FYPO:0000003"],"is_obsolete":false,"name":"decreased bipolar index","termid":"FYPO:0003535"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000002","FYPO:0000631","FYPO:0001320","FYPO:0005447","FYPO:0000032","FYPO:0001985","FYPO:0004803"],"is_obsolete":false,"name":"delayed onset of actomyosin contractile ring assembly","termid":"FYPO:0000729"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985"],"is_obsolete":false,"name":"mislocalized hyphal septum","termid":"FYPO:0007801"}],"references_by_uniquename":{},"uniquename":"SJAG_02392:allele-1"}


id: SJAG_02409:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"stc1","product":"CLRC complex-Argonaute linker protein Stc1","uniquename":"SJAG_02409"},"genes_by_uniquename":{},"genotypes":[{"display_name":"stc1delta","display_uniquename":"stc1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02409:allele-1","expression":"Null"}]}]}],"name":"stc1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":9,"genotype_count":9,"interesting_parent_ids":["FYPO:0002059","FYPO:0002057","FYPO:0000003"],"is_obsolete":false,"name":"inviable vegetative cell population","termid":"FYPO:0002061"}],"references_by_uniquename":{},"uniquename":"SJAG_02409:allele-1"}


id: SJAG_02486:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"ste6","product":"RasGEF Ste6","uniquename":"SJAG_02486"},"genes_by_uniquename":{},"genotypes":[{"display_name":"ste6delta","display_uniquename":"ste6delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02486:allele-1","expression":"Null"}]}]}],"name":"ste6delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":7,"genotype_count":6,"interesting_parent_ids":["FYPO:0000257"],"is_obsolete":false,"name":"normal hyphal growth","termid":"FYPO:0007790"},{"cv_name":"fission_yeast_phenotype","gene_count":10,"genotype_count":10,"interesting_parent_ids":["FYPO:0001985","FYPO:0000301","FYPO:0005447"],"is_obsolete":false,"name":"sterile","secondary_identifiers":["FYPO:0000638"],"termid":"FYPO:0000280"},{"cv_name":"fission_yeast_phenotype","gene_count":23,"genotype_count":24,"interesting_parent_ids":["FYPO:0000002","FYPO:0000004"],"is_obsolete":false,"name":"viable cell","termid":"FYPO:0000124"}],"references_by_uniquename":{},"uniquename":"SJAG_02486:allele-1"}


id: SJAG_02527:allele-1
data:
  {"allele_type":"disruption","alleles_by_uniquename":{},"description":"RNA sequencing revealed that only the 5' end of the disrupted allele showed low RNA expression and coverage","gene":{"name":"lig4","product":"DNA repair ligase Lig4","uniquename":"SJAG_02527"},"genes_by_uniquename":{},"genotypes":[{"display_name":"lig4::KanMX6(RNA sequencing revealed that only the 5' end of the disrupted allele showed low RNA expression and coverage)[Knockdown]","display_uniquename":"lig4::KanMX6-RNA_sequencing_revealed_that_only_the_5'_end_of_the_disrupted_allele_showed_low_RNA_expression_and_coverage-disruption-expression-knockdown","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02527:allele-1","expression":"Knockdown"}]}]}],"name":"lig4::KanMX6","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":6,"genotype_count":9,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"decreased hyphal length","termid":"FYPO:0007776"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0005447","FYPO:0000003","FYPO:0001985"],"is_obsolete":false,"name":"increased flocculation during vegetative growth","termid":"FYPO:0009111"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000003","FYPO:0001985"],"is_obsolete":false,"name":"increased sporulation frequency","termid":"FYPO:0000588"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0001985","FYPO:0000003","FYPO:0002057"],"is_obsolete":false,"name":"loss of viability following cellular response to UV","termid":"FYPO:0005705"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0002057","FYPO:0001985","FYPO:0000003"],"is_obsolete":false,"name":"loss of viability in stationary phase upon glucose starvation","termid":"FYPO:0005231"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0005447","FYPO:0001985","FYPO:0000003","FYPO:0000139","FYPO:0002683"],"is_obsolete":false,"name":"sensitive to caffeine during vegetative growth","termid":"FYPO:0000097"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0002058","FYPO:0002060","FYPO:0000003","FYPO:0005447","FYPO:0001985","FYPO:0000139","FYPO:0002057"],"is_obsolete":false,"name":"sensitive to heat shock","termid":"FYPO:0001408"}],"references_by_uniquename":{},"uniquename":"SJAG_02527:allele-1"}


id: SJAG_02576:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"rad25","product":"14-3-3 protein Rad25","uniquename":"SJAG_02576"},"genes_by_uniquename":{},"genotypes":[{"display_name":"rad25delta","display_uniquename":"rad25delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02576:allele-1","expression":"Null"}]}]}],"name":"rad25delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":8,"genotype_count":8,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"abolished hyphal transition","termid":"FYPO:0007782"}],"references_by_uniquename":{},"uniquename":"SJAG_02576:allele-1"}


id: SJAG_02592:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"sty1","product":"MAP kinase Sty1","uniquename":"SJAG_02592"},"genes_by_uniquename":{},"genotypes":[{"display_name":"SJAG_00124delta sty1delta","display_uniquename":"SJAG_00124delta__sty1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00124:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_02592:allele-1","expression":"Null"}]}]},{"display_name":"sty1delta","display_uniquename":"sty1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02592:allele-1","expression":"Null"}]}]}],"name":"sty1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0005447","FYPO:0000631","FYPO:0000032","FYPO:0001985","FYPO:0000002","FYPO:0004803","FYPO:0001320"],"is_obsolete":false,"name":"abolished actomyosin contractile ring assembly, clumped medial cortical nodes","termid":"FYPO:0002998"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0001985","FYPO:0002060","FYPO:0002057","FYPO:0000139","FYPO:0005447","FYPO:0000003","FYPO:0002058"],"is_obsolete":false,"name":"abolished cell population growth at high temperature","termid":"FYPO:0004481"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985","FYPO:0005447"],"is_obsolete":false,"name":"decreased quorum sensing response","termid":"FYPO:0007824"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0000005","FYPO:0001985","FYPO:0001320","FYPO:0000002"],"is_obsolete":false,"name":"elongated vegetative cell","termid":"FYPO:0001122"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000002","FYPO:0007773"],"is_obsolete":false,"name":"increased hyphal branching","termid":"FYPO:0007777"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"increased hyphal length","termid":"FYPO:0007775"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"increased invasive hyphal growth","termid":"FYPO:0007774"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"increased mycelial expansion","termid":"FYPO:0007779"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0002494","FYPO:0002274","FYPO:0001985","FYPO:0005447","FYPO:0000002"],"is_obsolete":false,"name":"increased protein phosphorylation","termid":"FYPO:0002680"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0005447","FYPO:0001985","FYPO:0000003","FYPO:0000139","FYPO:0002683"],"is_obsolete":false,"name":"sensitive to caffeine during vegetative growth","termid":"FYPO:0000097"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000003","FYPO:0005447","FYPO:0000139","FYPO:0001985","FYPO:0002683"],"is_obsolete":false,"name":"sensitive to latrunculin A","termid":"FYPO:0000107"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0001985","FYPO:0000003","FYPO:0000139","FYPO:0005447"],"is_obsolete":false,"name":"sensitive to oxidative stress","termid":"FYPO:0000726"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0005447","FYPO:0001985","FYPO:0000139","FYPO:0000003"],"is_obsolete":false,"name":"sensitive to salt stress","termid":"FYPO:0000271"},{"cv_name":"fission_yeast_phenotype","gene_count":10,"genotype_count":10,"interesting_parent_ids":["FYPO:0001985","FYPO:0000301","FYPO:0005447"],"is_obsolete":false,"name":"sterile","secondary_identifiers":["FYPO:0000638"],"termid":"FYPO:0000280"},{"cv_name":"fission_yeast_phenotype","gene_count":23,"genotype_count":24,"interesting_parent_ids":["FYPO:0000002","FYPO:0000004"],"is_obsolete":false,"name":"viable cell","termid":"FYPO:0000124"}],"references_by_uniquename":{},"uniquename":"SJAG_02592:allele-1"}


id: SJAG_02594:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"arb1","product":"argonaute inhibitor protein 1","uniquename":"SJAG_02594"},"genes_by_uniquename":{},"genotypes":[{"display_name":"arb1delta","display_uniquename":"arb1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02594:allele-1","expression":"Null"}]}]}],"name":"arb1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":9,"genotype_count":9,"interesting_parent_ids":["FYPO:0002059","FYPO:0002057","FYPO:0000003"],"is_obsolete":false,"name":"inviable vegetative cell population","termid":"FYPO:0002061"}],"references_by_uniquename":{},"uniquename":"SJAG_02594:allele-1"}


id: SJAG_02621:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"ago1","product":"argonaute","uniquename":"SJAG_02621"},"genes_by_uniquename":{},"genotypes":[{"display_name":"ago1delta","display_uniquename":"ago1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02621:allele-1","expression":"Null"}]}]},{"display_name":"ago1delta leo1delta","display_uniquename":"ago1delta__leo1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02621:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_03118:allele-1","expression":"Null"}]}]}],"name":"ago1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0001320","FYPO:0005447","FYPO:0006551","FYPO:0000002","FYPO:0001985"],"is_obsolete":false,"name":"increased centromeric transcript level","termid":"FYPO:0004982"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":2,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985","FYPO:0001320","FYPO:0006551","FYPO:0005447"],"is_obsolete":false,"name":"increased telomeric transcript level","termid":"FYPO:0004573"},{"cv_name":"fission_yeast_phenotype","gene_count":9,"genotype_count":9,"interesting_parent_ids":["FYPO:0002059","FYPO:0002057","FYPO:0000003"],"is_obsolete":false,"name":"inviable vegetative cell population","termid":"FYPO:0002061"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0000257","FYPO:0000002","FYPO:0001320","FYPO:0001312"],"is_obsolete":false,"name":"normal histone H3-K9 dimethylation at transposable element during vegetative growth","termid":"FYPO:0006989"},{"cv_name":"fission_yeast_phenotype","gene_count":31,"genotype_count":36,"interesting_parent_ids":["FYPO:0000003","FYPO:0002057"],"is_obsolete":false,"name":"viable cell population","termid":"FYPO:0002058"}],"references_by_uniquename":{},"uniquename":"SJAG_02621:allele-1"}


id: SJAG_02668:allele-1
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"R77W","gene":{"name":"mpe1","product":"mRNA cleavage ubiquitin-protein ligase E3 Mpe1","uniquename":"SJAG_02668"},"genes_by_uniquename":{},"genotypes":[{"display_name":"dcr1delta mpe1-R77W(aa)","display_uniquename":"dcr1delta__mpe1-R77W-R77W-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03689:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_02668:allele-1","expression":"Not assayed or wild type"}]}]}],"name":"mpe1-R77W","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":31,"genotype_count":36,"interesting_parent_ids":["FYPO:0000003","FYPO:0002057"],"is_obsolete":false,"name":"viable cell population","termid":"FYPO:0002058"}],"references_by_uniquename":{},"uniquename":"SJAG_02668:allele-1"}


id: SJAG_02695:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"tip1","product":"CLIP170 family protein Tip1","uniquename":"SJAG_02695"},"genes_by_uniquename":{},"genotypes":[{"display_name":"tip1delta","display_uniquename":"tip1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02695:allele-1","expression":"Null"}]}]}],"name":"tip1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":3,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985"],"is_obsolete":false,"name":"mislocalized septum","termid":"FYPO:0004293"}],"references_by_uniquename":{},"uniquename":"SJAG_02695:allele-1"}


id: SJAG_02763:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"ned1","product":"lipin, phosphatidate phosphatase Ned1","uniquename":"SJAG_02763"},"genes_by_uniquename":{},"genotypes":[{"display_name":"ned1delta","display_uniquename":"ned1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02763:allele-1","expression":"Null"}]}]}],"name":"ned1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":5,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985","FYPO:0005447","FYPO:0001320"],"is_obsolete":false,"name":"excess endoplasmic reticulum membrane present","termid":"FYPO:0001784"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":6,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0000002","FYPO:0002403","FYPO:0001320"],"is_obsolete":false,"name":"excess nuclear envelope present","termid":"FYPO:0001556"},{"cv_name":"fission_yeast_phenotype","gene_count":9,"genotype_count":9,"interesting_parent_ids":["FYPO:0000139","FYPO:0005447","FYPO:0000003","FYPO:0002058","FYPO:0002057","FYPO:0001985","FYPO:0002060"],"is_obsolete":false,"name":"slow vegetative cell population growth","termid":"FYPO:0001234"}],"references_by_uniquename":{},"uniquename":"SJAG_02763:allele-1"}


id: SJAG_02763:allele-2
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"D422E,D424E","gene":{"name":"ned1","product":"lipin, phosphatidate phosphatase Ned1","uniquename":"SJAG_02763"},"genes_by_uniquename":{},"genotypes":[{"display_name":"ned1cat(D422E,D424E aa)","display_uniquename":"ned1cat-D422E,D424E-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02763:allele-2","expression":"Not assayed or wild type"}]}]}],"name":"ned1cat","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":6,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0000002","FYPO:0002403","FYPO:0001320"],"is_obsolete":false,"name":"excess nuclear envelope present","termid":"FYPO:0001556"}],"references_by_uniquename":{},"synonyms":[{"name":"Catalytic mutant","reference":"PMID:26774782","type":"exact"}],"uniquename":"SJAG_02763:allele-2"}


id: SJAG_02763:allele-3
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"S235A; S651","gene":{"name":"ned1","product":"lipin, phosphatidate phosphatase Ned1","uniquename":"SJAG_02763"},"genes_by_uniquename":{},"genotypes":[{"display_name":"ned1S235A/S651(S235A; S651 aa)","display_uniquename":"ned1S235A_S651-S235A__S651-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02763:allele-3","expression":"Not assayed or wild type"}]}]}],"name":"ned1S235A/S651","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":2,"interesting_parent_ids":["FYPO:0000002","FYPO:0005447","FYPO:0001985"],"is_obsolete":false,"name":"increased lipid droplet formation","termid":"FYPO:0006945"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0001320","FYPO:0000002","FYPO:0000257"],"is_obsolete":false,"name":"normal nuclear envelope morphology","termid":"FYPO:0003751"}],"references_by_uniquename":{},"synonyms":[{"name":"refractory to phosphorylation","reference":"PMID:26774782","type":"exact"}],"uniquename":"SJAG_02763:allele-3"}


id: SJAG_02763:allele-4
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"S235E; S651E","gene":{"name":"ned1","product":"lipin, phosphatidate phosphatase Ned1","uniquename":"SJAG_02763"},"genes_by_uniquename":{},"genotypes":[{"display_name":"ned1S235E/S651E(S235E; S651E aa)","display_uniquename":"ned1S235E_S651E-S235E__S651E-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02763:allele-4","expression":"Not assayed or wild type"}]}]}],"name":"ned1S235E/S651E","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":2,"interesting_parent_ids":["FYPO:0000002","FYPO:0005447","FYPO:0001985"],"is_obsolete":false,"name":"decreased lipid droplet formation","termid":"FYPO:0006054"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":5,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985","FYPO:0005447","FYPO:0001320"],"is_obsolete":false,"name":"excess endoplasmic reticulum membrane present","termid":"FYPO:0001784"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":6,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0000002","FYPO:0002403","FYPO:0001320"],"is_obsolete":false,"name":"excess nuclear envelope present","termid":"FYPO:0001556"}],"references_by_uniquename":{},"synonyms":[{"name":"phosphomimetic","reference":"PMID:26774782","type":"exact"}],"uniquename":"SJAG_02763:allele-4"}


id: SJAG_02763:allele-5
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"S499E; S538E; T620E; T638E; S651E","gene":{"name":"ned1","product":"lipin, phosphatidate phosphatase Ned1","uniquename":"SJAG_02763"},"genes_by_uniquename":{},"genotypes":[{"display_name":"ned1-5E(S499E; S538E; T620E; T638E; S651E aa)","display_uniquename":"ned1-5E-S499E__S538E__T620E__T638E__S651E-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02763:allele-5","expression":"Not assayed or wild type"}]}]}],"name":"ned1-5E","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":2,"interesting_parent_ids":["FYPO:0000002","FYPO:0005447","FYPO:0001985"],"is_obsolete":false,"name":"decreased lipid droplet formation","termid":"FYPO:0006054"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":5,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985","FYPO:0005447","FYPO:0001320"],"is_obsolete":false,"name":"excess endoplasmic reticulum membrane present","termid":"FYPO:0001784"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":6,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0000002","FYPO:0002403","FYPO:0001320"],"is_obsolete":false,"name":"excess nuclear envelope present","termid":"FYPO:0001556"}],"references_by_uniquename":{},"uniquename":"SJAG_02763:allele-5"}


id: SJAG_02763:allele-6
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"S499A; S538A; T620A; T638A; S651A","gene":{"name":"ned1","product":"lipin, phosphatidate phosphatase Ned1","uniquename":"SJAG_02763"},"genes_by_uniquename":{},"genotypes":[{"display_name":"ned1-5A(S499A; S538A; T620A; T638A; S651A aa)","display_uniquename":"ned1-5A-S499A__S538A__T620A__T638A__S651A-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02763:allele-6","expression":"Not assayed or wild type"}]}]}],"name":"ned1-5A","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":2,"interesting_parent_ids":["FYPO:0000002","FYPO:0005447","FYPO:0001985"],"is_obsolete":false,"name":"increased lipid droplet formation","termid":"FYPO:0006945"}],"references_by_uniquename":{},"synonyms":[{"name":"refractory to potential proline-directed kinase phosphorylation","reference":"PMID:26774782","type":"exact"}],"uniquename":"SJAG_02763:allele-6"}


id: SJAG_02771:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"rad1","product":"checkpoint clamp complex protein Rad1","uniquename":"SJAG_02771"},"genes_by_uniquename":{},"genotypes":[{"display_name":"rad1delta","display_uniquename":"rad1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02771:allele-1","expression":"Null"}]}]}],"name":"rad1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":8,"genotype_count":8,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"abolished hyphal transition","termid":"FYPO:0007782"}],"references_by_uniquename":{},"uniquename":"SJAG_02771:allele-1"}


id: SJAG_02796:allele-1
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"A343T","gene":{"name":"pyk1","product":"pyruvate kinase","uniquename":"SJAG_02796"},"genes_by_uniquename":{},"genotypes":[{"display_name":"pyk1-A343T(aa)","display_uniquename":"pyk1-A343T-A343T-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02796:allele-1","expression":"Not assayed or wild type"}]}]}],"name":"pyk1-A343T","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000654","FYPO:0000652","FYPO:0005447","FYPO:0000707","FYPO:0001985","FYPO:0000002","FYPO:0000661"],"is_obsolete":false,"name":"abnormal pyruvate kinase activity","termid":"FYPO:0007363"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000257","FYPO:0001320","FYPO:0000002"],"is_obsolete":false,"name":"normal glucose consumption","termid":"FYPO:0004165"}],"references_by_uniquename":{},"uniquename":"SJAG_02796:allele-1"}


id: SJAG_02819:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"efc25","product":"Ras1 GEF Efc25","uniquename":"SJAG_02819"},"genes_by_uniquename":{},"genotypes":[{"display_name":"efc25delta","display_uniquename":"efc25delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02819:allele-1","expression":"Null"}]}]}],"name":"efc25delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":5,"interesting_parent_ids":["FYPO:0000002","FYPO:0007773","FYPO:0001985","FYPO:0005447"],"is_obsolete":false,"name":"abolished hyphal growth","termid":"FYPO:0007789"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000003","FYPO:0001985"],"is_obsolete":false,"name":"decreased sporulation frequency","termid":"FYPO:0000584"},{"cv_name":"fission_yeast_phenotype","gene_count":23,"genotype_count":24,"interesting_parent_ids":["FYPO:0000002","FYPO:0000004"],"is_obsolete":false,"name":"viable cell","termid":"FYPO:0000124"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0001985","FYPO:0000002","FYPO:0000005","FYPO:0001320","FYPO:0000004"],"is_obsolete":false,"name":"viable spheroid vegetative cell","termid":"FYPO:0002380"}],"references_by_uniquename":{},"uniquename":"SJAG_02819:allele-1"}


id: SJAG_02860:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"wcs1","product":"blue light photoreceptor Wcs1","uniquename":"SJAG_02860"},"genes_by_uniquename":{},"genotypes":[{"display_name":"wcs1delta","display_uniquename":"wcs1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02860:allele-1","expression":"Null"}]}]}],"name":"wcs1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000298","FYPO:0000002"],"is_obsolete":false,"name":"abnormal blue light response","termid":"FYPO:0008031"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000003"],"is_obsolete":false,"name":"decreased sexual agglutination in response to blue light","termid":"FYPO:0008046"}],"references_by_uniquename":{},"uniquename":"SJAG_02860:allele-1"}


id: SJAG_02923:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"spk1","product":"MAP kinase Spk1","uniquename":"SJAG_02923"},"genes_by_uniquename":{},"genotypes":[{"display_name":"spk1delta","display_uniquename":"spk1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02923:allele-1","expression":"Null"}]}]}],"name":"spk1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":25,"genotype_count":31,"interesting_parent_ids":["FYPO:0000002"],"is_obsolete":false,"name":"abnormal hyphal growth","termid":"FYPO:0007773"},{"cv_name":"fission_yeast_phenotype","gene_count":10,"genotype_count":10,"interesting_parent_ids":["FYPO:0001985","FYPO:0000301","FYPO:0005447"],"is_obsolete":false,"name":"sterile","secondary_identifiers":["FYPO:0000638"],"termid":"FYPO:0000280"},{"cv_name":"fission_yeast_phenotype","gene_count":23,"genotype_count":24,"interesting_parent_ids":["FYPO:0000002","FYPO:0000004"],"is_obsolete":false,"name":"viable cell","termid":"FYPO:0000124"}],"references_by_uniquename":{},"uniquename":"SJAG_02923:allele-1"}


id: SJAG_03048:allele-1
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"G146D","gene":{"name":"cdc2","product":"cyclin-dependent protein kinase Cdk1/Cdc2","uniquename":"SJAG_03048"},"genes_by_uniquename":{},"genotypes":[{"display_name":"cdc2-1w(G146D aa)","display_uniquename":"cdc2-1w-G146D-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03048:allele-1","expression":"Not assayed or wild type"}]}]}],"name":"cdc2-1w","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":4,"interesting_parent_ids":["FYPO:0001985","FYPO:0000004","FYPO:0000002","FYPO:0000005","FYPO:0001320"],"is_obsolete":false,"name":"viable small vegetative cell","termid":"FYPO:0000648"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0000004","FYPO:0000005","FYPO:0000002","FYPO:0001985","FYPO:0001320"],"is_obsolete":false,"name":"viable thin vegetative cell","termid":"FYPO:0001877"}],"references_by_uniquename":{},"uniquename":"SJAG_03048:allele-1"}


id: SJAG_03118:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"leo1","product":"RNA polymerase II associated Paf1 complex subunit Leo1","uniquename":"SJAG_03118"},"genes_by_uniquename":{},"genotypes":[{"display_name":"ago1delta leo1delta","display_uniquename":"ago1delta__leo1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02621:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_03118:allele-1","expression":"Null"}]}]},{"display_name":"clr4delta leo1delta","display_uniquename":"clr4delta__leo1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04574:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_03118:allele-1","expression":"Null"}]}]},{"display_name":"dcr1delta leo1delta","display_uniquename":"dcr1delta__leo1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03689:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_03118:allele-1","expression":"Null"}]}]}],"name":"leo1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0001320","FYPO:0005447","FYPO:0006551","FYPO:0000002","FYPO:0001985"],"is_obsolete":false,"name":"increased centromeric transcript level","termid":"FYPO:0004982"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":2,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985","FYPO:0001320","FYPO:0006551","FYPO:0005447"],"is_obsolete":false,"name":"increased telomeric transcript level","termid":"FYPO:0004573"},{"cv_name":"fission_yeast_phenotype","gene_count":9,"genotype_count":9,"interesting_parent_ids":["FYPO:0002059","FYPO:0002057","FYPO:0000003"],"is_obsolete":false,"name":"inviable vegetative cell population","termid":"FYPO:0002061"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0000257","FYPO:0000002","FYPO:0001320","FYPO:0001312"],"is_obsolete":false,"name":"normal histone H3-K9 dimethylation at transposable element during vegetative growth","termid":"FYPO:0006989"},{"cv_name":"fission_yeast_phenotype","gene_count":31,"genotype_count":36,"interesting_parent_ids":["FYPO:0000003","FYPO:0002057"],"is_obsolete":false,"name":"viable cell population","termid":"FYPO:0002058"}],"references_by_uniquename":{},"uniquename":"SJAG_03118:allele-1"}


id: SJAG_03118:allele-2
data:
  {"allele_type":"nonsense_mutation","alleles_by_uniquename":{},"description":"aa61->stop","gene":{"name":"leo1","product":"RNA polymerase II associated Paf1 complex subunit Leo1","uniquename":"SJAG_03118"},"genes_by_uniquename":{},"genotypes":[{"display_name":"dcr1delta frameshift(aa61->stop)","display_uniquename":"dcr1delta__frameshift-aa61->stop-nonsense_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03689:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_03118:allele-2","expression":"Not assayed or wild type"}]}]}],"name":"frameshift","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":31,"genotype_count":36,"interesting_parent_ids":["FYPO:0000003","FYPO:0002057"],"is_obsolete":false,"name":"viable cell population","termid":"FYPO:0002058"}],"references_by_uniquename":{},"uniquename":"SJAG_03118:allele-2"}


id: SJAG_03143:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"sep1","product":"DNA-binding forkhead transcription factor Sep1","uniquename":"SJAG_03143"},"genes_by_uniquename":{},"genotypes":[{"display_name":"sep1delta","display_uniquename":"sep1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03143:allele-1","expression":"Null"}]}]}],"name":"sep1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0001985","FYPO:0000002","FYPO:0001320","FYPO:0005447"],"is_obsolete":false,"name":"abolished cell separation after cytokinesis","termid":"FYPO:0006557"}],"references_by_uniquename":{},"uniquename":"SJAG_03143:allele-1"}


id: SJAG_03164:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"ura4","product":"orotidine 5'-phosphate decarboxylase Ura4","uniquename":"SJAG_03164"},"genes_by_uniquename":{},"genotypes":[{"display_name":"ura4delta","display_uniquename":"ura4delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03164:allele-1","expression":"Null"}]}]}],"name":"ura4delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0001985","FYPO:0000002"],"is_obsolete":false,"name":"growth auxotrophic for uracil","termid":"FYPO:0001012"}],"references_by_uniquename":{},"uniquename":"SJAG_03164:allele-1"}


id: SJAG_03198:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"oar2","product":"3-oxoacyl-[acyl-carrier-protein] reductase Oar2","uniquename":"SJAG_03198"},"genes_by_uniquename":{},"genotypes":[{"display_name":"apc2-361(E548K ts) oar2delta","display_uniquename":"apc2-361-E548K_ts-amino_acid_mutation-expression-not_assayed_or_wild_type__oar2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03575:allele-1","expression":"Not assayed or wild type"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_03198:allele-1","expression":"Null"}]}]},{"display_name":"oar2delta","display_uniquename":"oar2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03198:allele-1","expression":"Null"}]}]}],"name":"oar2delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":5,"interesting_parent_ids":["FYPO:0000139","FYPO:0002060","FYPO:0002057","FYPO:0001985","FYPO:0002058","FYPO:0005447","FYPO:0000003"],"is_obsolete":false,"name":"decreased cell population growth at low temperature","secondary_identifiers":["FYPO:0002175"],"termid":"FYPO:0000080"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":4,"interesting_parent_ids":["FYPO:0002058","FYPO:0002057","FYPO:0000003","FYPO:0000139","FYPO:0000257","FYPO:0002060"],"is_obsolete":false,"name":"normal cell population growth at high temperature","termid":"FYPO:0000674"}],"references_by_uniquename":{},"uniquename":"SJAG_03198:allele-1"}


id: SJAG_03198:allele-2
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"Y189D cold sensitive","gene":{"name":"oar2","product":"3-oxoacyl-[acyl-carrier-protein] reductase Oar2","uniquename":"SJAG_03198"},"genes_by_uniquename":{},"genotypes":[{"display_name":"apc2-361(E548K ts) oar2-51(Y189D cold sensitive)","display_uniquename":"apc2-361-E548K_ts-amino_acid_mutation-expression-not_assayed_or_wild_type__oar2-51-Y189D_cold_sensitive-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03575:allele-1","expression":"Not assayed or wild type"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_03198:allele-2","expression":"Not assayed or wild type"}]}]},{"display_name":"oar2-51(Y189D cold sensitive)[Overexpression]","display_uniquename":"oar2-51-Y189D_cold_sensitive-amino_acid_mutation-expression-overexpression","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03198:allele-2","expression":"Overexpression"}]}]}],"name":"oar2-51","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":5,"interesting_parent_ids":["FYPO:0000139","FYPO:0002060","FYPO:0002057","FYPO:0001985","FYPO:0002058","FYPO:0005447","FYPO:0000003"],"is_obsolete":false,"name":"decreased cell population growth at low temperature","secondary_identifiers":["FYPO:0002175"],"termid":"FYPO:0000080"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":4,"interesting_parent_ids":["FYPO:0002058","FYPO:0002057","FYPO:0000003","FYPO:0000139","FYPO:0000257","FYPO:0002060"],"is_obsolete":false,"name":"normal cell population growth at high temperature","termid":"FYPO:0000674"}],"references_by_uniquename":{},"uniquename":"SJAG_03198:allele-2"}


id: SJAG_03198:allele-3
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"R72A,L75A,R106A,L109A","gene":{"name":"oar2","product":"3-oxoacyl-[acyl-carrier-protein] reductase Oar2","uniquename":"SJAG_03198"},"genes_by_uniquename":{},"genotypes":[{"display_name":"apc2-361(E548K ts) oar2-dm12(R72A,L75A,R106A,L109A aa)[Overexpression]","display_uniquename":"apc2-361-E548K_ts-amino_acid_mutation-expression-not_assayed_or_wild_type__oar2-dm12-R72A,L75A,R106A,L109A-amino_acid_mutation-expression-overexpression","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03575:allele-1","expression":"Not assayed or wild type"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_03198:allele-3","expression":"Overexpression"}]}]},{"display_name":"oar2-dm12(R72A,L75A,R106A,L109A aa)[Overexpression]","display_uniquename":"oar2-dm12-R72A,L75A,R106A,L109A-amino_acid_mutation-expression-overexpression","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03198:allele-3","expression":"Overexpression"}]}]}],"name":"oar2-dm12","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":5,"interesting_parent_ids":["FYPO:0000139","FYPO:0002060","FYPO:0002057","FYPO:0001985","FYPO:0002058","FYPO:0005447","FYPO:0000003"],"is_obsolete":false,"name":"decreased cell population growth at low temperature","secondary_identifiers":["FYPO:0002175"],"termid":"FYPO:0000080"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":4,"interesting_parent_ids":["FYPO:0002058","FYPO:0002057","FYPO:0000003","FYPO:0000139","FYPO:0000257","FYPO:0002060"],"is_obsolete":false,"name":"normal cell population growth at high temperature","termid":"FYPO:0000674"}],"references_by_uniquename":{},"synonyms":[{"name":"destruction box mutations","reference":"PMID:23786411","type":"exact"}],"uniquename":"SJAG_03198:allele-3"}


id: SJAG_03250:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"sst4","product":"sorting receptor for ubiquitinated membrane proteins, ESCRT 0 complex subunit Sst4","uniquename":"SJAG_03250"},"genes_by_uniquename":{},"genotypes":[{"display_name":"sst4delta","display_uniquename":"sst4delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03250:allele-1","expression":"Null"}]}]}],"name":"sst4delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0000138","FYPO:0000002"],"is_obsolete":false,"name":"abnormal protein complex localization","termid":"FYPO:0001440"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000002","FYPO:0001320","FYPO:0000257"],"is_obsolete":false,"name":"normal nuclear envelope morphology during mitosis","termid":"FYPO:0003780"}],"references_by_uniquename":{},"uniquename":"SJAG_03250:allele-1"}


id: SJAG_03351:allele-1
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"A186T ts","gene":{"name":"cut23","product":"anaphase-promoting complex TPR lobe subcomplex subunit Apc8","uniquename":"SJAG_03351"},"genes_by_uniquename":{},"genotypes":[{"display_name":"cut23-415(A186T ts)","display_uniquename":"cut23-415-A186T_ts-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03351:allele-1","expression":"Not assayed or wild type"}]}]}],"name":"cut23-415","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0000631","FYPO:0005447","FYPO:0000002","FYPO:0001320","FYPO:0001985"],"is_obsolete":false,"name":"increased duration of mitotic anaphase","secondary_identifiers":["FYPO:0000617"],"termid":"FYPO:0000618"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0001985","FYPO:0001320","FYPO:0000002","FYPO:0005447","FYPO:0006551"],"is_obsolete":false,"name":"increased protein level during vegetative growth","secondary_identifiers":["FYPO:0000762"],"termid":"FYPO:0001327"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0002057","FYPO:0001985","FYPO:0000003"],"is_obsolete":false,"name":"loss of viability at high temperature","termid":"FYPO:0001387"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0001985","FYPO:0000631","FYPO:0005447","FYPO:0000002","FYPO:0001320"],"is_obsolete":false,"name":"mitotic metaphase/anaphase transition delay","secondary_identifiers":["FYPO:0000393","FYPO:0004309"],"termid":"FYPO:0000324"}],"references_by_uniquename":{},"uniquename":"SJAG_03351:allele-1"}


id: SJAG_03356:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"rdp1","product":"RNA-directed RNA polymerase (RDRC) Rdp1","uniquename":"SJAG_03356"},"genes_by_uniquename":{},"genotypes":[{"display_name":"rdp1delta","display_uniquename":"rdp1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03356:allele-1","expression":"Null"}]}]}],"name":"rdp1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":9,"genotype_count":9,"interesting_parent_ids":["FYPO:0002059","FYPO:0002057","FYPO:0000003"],"is_obsolete":false,"name":"inviable vegetative cell population","termid":"FYPO:0002061"}],"references_by_uniquename":{},"uniquename":"SJAG_03356:allele-1"}


id: SJAG_03384:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"wis4","product":"MAP kinase kinase kinase Wis4","uniquename":"SJAG_03384"},"genes_by_uniquename":{},"genotypes":[{"display_name":"wis4delta","display_uniquename":"wis4delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03384:allele-1","expression":"Null"}]}]}],"name":"wis4delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":23,"genotype_count":24,"interesting_parent_ids":["FYPO:0000002","FYPO:0000004"],"is_obsolete":false,"name":"viable cell","termid":"FYPO:0000124"}],"references_by_uniquename":{},"uniquename":"SJAG_03384:allele-1"}


id: SJAG_03444:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"agn1","product":"cell wall glucan endo-1,3-alpha-glucosidase Agn1","uniquename":"SJAG_03444"},"genes_by_uniquename":{},"genotypes":[{"display_name":"agn1delta","display_uniquename":"agn1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03444:allele-1","expression":"Null"}]}]}],"name":"agn1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985","FYPO:0005447","FYPO:0001320"],"is_obsolete":false,"name":"abnormal cell separation after cytokinesis resulting in chained cells","termid":"FYPO:0001971"}],"references_by_uniquename":{},"uniquename":"SJAG_03444:allele-1"}


id: SJAG_03449:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"man1","product":"LEM domain nuclear inner membrane protein Man1, Sad1 interacting factor","uniquename":"SJAG_03449"},"genes_by_uniquename":{},"genotypes":[{"display_name":"man1delta","display_uniquename":"man1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03449:allele-1","expression":"Null"}]}]}],"name":"man1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":4,"interesting_parent_ids":["FYPO:0005447","FYPO:0001985","FYPO:0001320","FYPO:0000002","FYPO:0002403"],"is_obsolete":false,"name":"abnormal nuclear envelope morphology during mitosis","termid":"FYPO:0003779"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":3,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985","FYPO:0001320","FYPO:0000631","FYPO:0000641","FYPO:0000337","FYPO:0005447"],"is_obsolete":false,"name":"abnormal rDNA separation","termid":"FYPO:0000671"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":4,"interesting_parent_ids":["FYPO:0001985","FYPO:0000138","FYPO:0001320","FYPO:0000002","FYPO:0005447"],"is_obsolete":false,"name":"increased telomere-nuclear envelope distance during mitosis","termid":"FYPO:0004890"},{"cv_name":"fission_yeast_phenotype","gene_count":9,"genotype_count":9,"interesting_parent_ids":["FYPO:0000139","FYPO:0005447","FYPO:0000003","FYPO:0002058","FYPO:0002057","FYPO:0001985","FYPO:0002060"],"is_obsolete":false,"name":"slow vegetative cell population growth","termid":"FYPO:0001234"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0001320","FYPO:0000002"],"is_obsolete":false,"name":"unequal nucleolus inheritance","termid":"FYPO:0004683"}],"references_by_uniquename":{},"uniquename":"SJAG_03449:allele-1"}


id: SJAG_03449:allele-2
data:
  {"allele_type":"partial_amino_acid_deletion","alleles_by_uniquename":{},"description":"man1DHEH","gene":{"name":"man1","product":"LEM domain nuclear inner membrane protein Man1, Sad1 interacting factor","uniquename":"SJAG_03449"},"genes_by_uniquename":{},"genotypes":[{"display_name":"Man1DHEH(man1DHEH)","display_uniquename":"Man1DHEH-man1DHEH-partial_amino_acid_deletion-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03449:allele-2","expression":"Not assayed or wild type"}]}]}],"name":"Man1DHEH","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":4,"interesting_parent_ids":["FYPO:0005447","FYPO:0001985","FYPO:0001320","FYPO:0000002","FYPO:0002403"],"is_obsolete":false,"name":"abnormal nuclear envelope morphology during mitosis","termid":"FYPO:0003779"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":3,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985","FYPO:0001320","FYPO:0000631","FYPO:0000641","FYPO:0000337","FYPO:0005447"],"is_obsolete":false,"name":"abnormal rDNA separation","termid":"FYPO:0000671"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":4,"interesting_parent_ids":["FYPO:0001985","FYPO:0000138","FYPO:0001320","FYPO:0000002","FYPO:0005447"],"is_obsolete":false,"name":"increased telomere-nuclear envelope distance during mitosis","termid":"FYPO:0004890"}],"references_by_uniquename":{},"uniquename":"SJAG_03449:allele-2"}


id: SJAG_03449:allele-3
data:
  {"allele_type":"amino_acid_insertion_and_deletion","alleles_by_uniquename":{},"description":"man1-1-488","gene":{"name":"man1","product":"LEM domain nuclear inner membrane protein Man1, Sad1 interacting factor","uniquename":"SJAG_03449"},"genes_by_uniquename":{},"genotypes":[{"display_name":"man1-1-488(aa)","display_uniquename":"man1-1-488-man1-1-488-amino_acid_insertion_and_deletion-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03449:allele-3","expression":"Not assayed or wild type"}]}]}],"name":"man1-1-488","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":4,"interesting_parent_ids":["FYPO:0005447","FYPO:0001985","FYPO:0001320","FYPO:0000002","FYPO:0002403"],"is_obsolete":false,"name":"abnormal nuclear envelope morphology during mitosis","termid":"FYPO:0003779"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":3,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985","FYPO:0001320","FYPO:0000631","FYPO:0000641","FYPO:0000337","FYPO:0005447"],"is_obsolete":false,"name":"abnormal rDNA separation","termid":"FYPO:0000671"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":4,"interesting_parent_ids":["FYPO:0001985","FYPO:0000138","FYPO:0001320","FYPO:0000002","FYPO:0005447"],"is_obsolete":false,"name":"increased telomere-nuclear envelope distance during mitosis","termid":"FYPO:0004890"}],"references_by_uniquename":{},"uniquename":"SJAG_03449:allele-3"}


id: SJAG_03453:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"ras1","product":"GTPase Ras1","uniquename":"SJAG_03453"},"genes_by_uniquename":{},"genotypes":[{"display_name":"ras1delta","display_uniquename":"ras1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03453:allele-1","expression":"Null"}]}]}],"name":"ras1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":5,"interesting_parent_ids":["FYPO:0000002","FYPO:0007773","FYPO:0001985","FYPO:0005447"],"is_obsolete":false,"name":"abolished hyphal growth","termid":"FYPO:0007789"},{"cv_name":"fission_yeast_phenotype","gene_count":13,"genotype_count":15,"interesting_parent_ids":["FYPO:0002058","FYPO:0005447","FYPO:0001985","FYPO:0002060","FYPO:0002057","FYPO:0000139","FYPO:0000003"],"is_obsolete":false,"name":"decreased vegetative cell population growth","termid":"FYPO:0001355"},{"cv_name":"fission_yeast_phenotype","gene_count":10,"genotype_count":10,"interesting_parent_ids":["FYPO:0001985","FYPO:0000301","FYPO:0005447"],"is_obsolete":false,"name":"sterile","secondary_identifiers":["FYPO:0000638"],"termid":"FYPO:0000280"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0001985","FYPO:0000002","FYPO:0000005","FYPO:0001320","FYPO:0000004"],"is_obsolete":false,"name":"viable spheroid vegetative cell","termid":"FYPO:0002380"}],"references_by_uniquename":{},"uniquename":"SJAG_03453:allele-1"}


id: SJAG_03575:allele-1
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"E548K ts","gene":{"name":"apc2","product":"anaphase-promoting complex cullin family subunit Apc2","uniquename":"SJAG_03575"},"genes_by_uniquename":{},"genotypes":[{"display_name":"apc2-361(E548K ts)","display_uniquename":"apc2-361-E548K_ts-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03575:allele-1","expression":"Not assayed or wild type"}]}]},{"display_name":"apc2-361(E548K ts) cem1delta","display_uniquename":"apc2-361-E548K_ts-amino_acid_mutation-expression-not_assayed_or_wild_type__cem1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03575:allele-1","expression":"Not assayed or wild type"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_01946:allele-1","expression":"Null"}]}]},{"display_name":"apc2-361(E548K ts) oar2-51(Y189D cold sensitive)","display_uniquename":"apc2-361-E548K_ts-amino_acid_mutation-expression-not_assayed_or_wild_type__oar2-51-Y189D_cold_sensitive-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03575:allele-1","expression":"Not assayed or wild type"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_03198:allele-2","expression":"Not assayed or wild type"}]}]},{"display_name":"apc2-361(E548K ts) oar2-dm12(R72A,L75A,R106A,L109A aa)[Overexpression]","display_uniquename":"apc2-361-E548K_ts-amino_acid_mutation-expression-not_assayed_or_wild_type__oar2-dm12-R72A,L75A,R106A,L109A-amino_acid_mutation-expression-overexpression","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03575:allele-1","expression":"Not assayed or wild type"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_03198:allele-3","expression":"Overexpression"}]}]},{"display_name":"apc2-361(E548K ts) oar2delta","display_uniquename":"apc2-361-E548K_ts-amino_acid_mutation-expression-not_assayed_or_wild_type__oar2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03575:allele-1","expression":"Not assayed or wild type"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_03198:allele-1","expression":"Null"}]}]}],"name":"apc2-361","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0000631","FYPO:0005447","FYPO:0000002","FYPO:0001320","FYPO:0001985"],"is_obsolete":false,"name":"increased duration of mitotic anaphase","secondary_identifiers":["FYPO:0000617"],"termid":"FYPO:0000618"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0001985","FYPO:0001320","FYPO:0000002","FYPO:0005447","FYPO:0006551"],"is_obsolete":false,"name":"increased protein level during vegetative growth","secondary_identifiers":["FYPO:0000762"],"termid":"FYPO:0001327"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0002057","FYPO:0001985","FYPO:0000003"],"is_obsolete":false,"name":"loss of viability at high temperature","termid":"FYPO:0001387"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0001985","FYPO:0000631","FYPO:0005447","FYPO:0000002","FYPO:0001320"],"is_obsolete":false,"name":"mitotic metaphase/anaphase transition delay","secondary_identifiers":["FYPO:0000393","FYPO:0004309"],"termid":"FYPO:0000324"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":4,"interesting_parent_ids":["FYPO:0002058","FYPO:0002057","FYPO:0000003","FYPO:0000139","FYPO:0000257","FYPO:0002060"],"is_obsolete":false,"name":"normal cell population growth at high temperature","termid":"FYPO:0000674"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0001320","FYPO:0000002"],"is_obsolete":false,"name":"unequal nucleolus inheritance","termid":"FYPO:0004683"}],"references_by_uniquename":{},"uniquename":"SJAG_03575:allele-1"}


id: SJAG_03581:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"gef1","product":"RhoGEF for Cdc42 (Gef1)","uniquename":"SJAG_03581"},"genes_by_uniquename":{},"genotypes":[{"display_name":"gef1delta","display_uniquename":"gef1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03581:allele-1","expression":"Null"}]}]}],"name":"gef1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0000004","FYPO:0000005","FYPO:0000002","FYPO:0001985","FYPO:0001320"],"is_obsolete":false,"name":"viable thin vegetative cell","termid":"FYPO:0001877"}],"references_by_uniquename":{},"uniquename":"SJAG_03581:allele-1"}


id: SJAG_03596:allele-1
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"L264P,P442T","gene":{"name":"cdc15","product":"F-BAR domain protein Cdc15","uniquename":"SJAG_03596"},"genes_by_uniquename":{},"genotypes":[{"display_name":"cdc15-A(L264P,P442T aa)","display_uniquename":"cdc15-A-L264P,P442T-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03596:allele-1","expression":"Not assayed or wild type"}]}]},{"display_name":"cdc15-A(L264P,P442T aa) mid1delta","display_uniquename":"cdc15-A-L264P,P442T-amino_acid_mutation-expression-not_assayed_or_wild_type__mid1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03596:allele-1","expression":"Not assayed or wild type"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_01143:allele-1","expression":"Null"}]}]}],"name":"cdc15-A","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0001985","FYPO:0000032","FYPO:0000002","FYPO:0005447","FYPO:0004803","FYPO:0001320","FYPO:0000631"],"is_obsolete":false,"name":"abnormal actomyosin contractile ring myosin filament organization","termid":"FYPO:0000231"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":1,"interesting_parent_ids":["FYPO:0000138","FYPO:0000002","FYPO:0001320","FYPO:0005447","FYPO:0001985"],"is_obsolete":false,"name":"delayed onset of protein localization to cell division site","termid":"FYPO:0003946"}],"references_by_uniquename":{},"uniquename":"SJAG_03596:allele-1"}


id: SJAG_03689:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"dcr1","product":"dicer","uniquename":"SJAG_03689"},"genes_by_uniquename":{},"genotypes":[{"display_name":"dcr1delta","display_uniquename":"dcr1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03689:allele-1","expression":"Null"}]}]},{"display_name":"dcr1delta frameshift(aa61->stop)","display_uniquename":"dcr1delta__frameshift-aa61->stop-nonsense_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03689:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_03118:allele-2","expression":"Not assayed or wild type"}]}]},{"display_name":"dcr1delta leo1delta","display_uniquename":"dcr1delta__leo1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03689:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_03118:allele-1","expression":"Null"}]}]},{"display_name":"dcr1delta mpe1-R77W(aa)","display_uniquename":"dcr1delta__mpe1-R77W-R77W-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03689:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_02668:allele-1","expression":"Not assayed or wild type"}]}]}],"name":"dcr1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0001320","FYPO:0006551","FYPO:0000002","FYPO:0005447","FYPO:0001985"],"is_obsolete":false,"name":"decreased siRNA level","termid":"FYPO:0004205"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0001320","FYPO:0005447","FYPO:0006551","FYPO:0000002","FYPO:0001985"],"is_obsolete":false,"name":"increased centromeric transcript level","termid":"FYPO:0004982"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0001320","FYPO:0000002"],"is_obsolete":false,"name":"increased rate of transposition","termid":"FYPO:0000595"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0001320","FYPO:0000002","FYPO:0006551","FYPO:0001985","FYPO:0005447"],"is_obsolete":false,"name":"increased subtelomere-derived RNA level","termid":"FYPO:0004574"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":2,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985","FYPO:0001320","FYPO:0006551","FYPO:0005447"],"is_obsolete":false,"name":"increased telomeric transcript level","termid":"FYPO:0004573"},{"cv_name":"fission_yeast_phenotype","gene_count":9,"genotype_count":9,"interesting_parent_ids":["FYPO:0002059","FYPO:0002057","FYPO:0000003"],"is_obsolete":false,"name":"inviable vegetative cell population","termid":"FYPO:0002061"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0000257","FYPO:0000002","FYPO:0001320","FYPO:0001312"],"is_obsolete":false,"name":"normal histone H3-K9 dimethylation at transposable element during vegetative growth","termid":"FYPO:0006989"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0001312","FYPO:0000257","FYPO:0001320","FYPO:0000002"],"is_obsolete":false,"name":"normal transposition","termid":"FYPO:0000596"},{"cv_name":"fission_yeast_phenotype","gene_count":31,"genotype_count":36,"interesting_parent_ids":["FYPO:0000003","FYPO:0002057"],"is_obsolete":false,"name":"viable cell population","termid":"FYPO:0002058"}],"references_by_uniquename":{},"uniquename":"SJAG_03689:allele-1"}


id: SJAG_03720:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"tel1","product":"ATM checkpoint kinase","uniquename":"SJAG_03720"},"genes_by_uniquename":{},"genotypes":[{"display_name":"tel1delta","display_uniquename":"tel1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03720:allele-1","expression":"Null"}]}]}],"name":"tel1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"increased invasive hyphal growth","termid":"FYPO:0007774"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000257","FYPO:0002060","FYPO:0002057","FYPO:0002058","FYPO:0000003","FYPO:0000139"],"is_obsolete":false,"name":"normal growth on camptothecin","termid":"FYPO:0001690"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0002057","FYPO:0000003","FYPO:0000257"],"is_obsolete":false,"name":"normal viability following cellular response to hydroxyurea","termid":"FYPO:0004693"}],"references_by_uniquename":{},"uniquename":"SJAG_03720:allele-1"}


id: SJAG_03753:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"mkh1","product":"MEK kinase (MEKK) Mkh1","uniquename":"SJAG_03753"},"genes_by_uniquename":{},"genotypes":[{"display_name":"mkh1delta","display_uniquename":"mkh1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03753:allele-1","expression":"Null"}]}]}],"name":"mkh1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":23,"genotype_count":24,"interesting_parent_ids":["FYPO:0000002","FYPO:0000004"],"is_obsolete":false,"name":"viable cell","termid":"FYPO:0000124"}],"references_by_uniquename":{},"uniquename":"SJAG_03753:allele-1"}


id: SJAG_03783:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"vps24","product":"ESCRT III complex subunit Vps24","uniquename":"SJAG_03783"},"genes_by_uniquename":{},"genotypes":[{"display_name":"vps24delta","display_uniquename":"vps24delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03783:allele-1","expression":"Null"}]}]}],"name":"vps24delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":13,"genotype_count":18,"interesting_parent_ids":["FYPO:0005447","FYPO:0000138","FYPO:0001985","FYPO:0000002"],"is_obsolete":false,"name":"abnormal protein localization","termid":"FYPO:0001370"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":7,"interesting_parent_ids":["FYPO:0001320","FYPO:0005447","FYPO:0000138","FYPO:0000002","FYPO:0001985"],"is_obsolete":false,"name":"abnormally clustered protein localization to nuclear envelope","termid":"FYPO:0007771"}],"references_by_uniquename":{},"uniquename":"SJAG_03783:allele-1"}


id: SJAG_03891:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"alx1","product":"ESCRT-III regulator Alx1","uniquename":"SJAG_03891"},"genes_by_uniquename":{},"genotypes":[{"display_name":"alx1delta","display_uniquename":"alx1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03891:allele-1","expression":"Null"}]}]}],"name":"alx1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":5,"interesting_parent_ids":["FYPO:0001985","FYPO:0000002","FYPO:0001320","FYPO:0005447"],"is_obsolete":false,"name":"delayed onset of mitotic nuclear membrane reassembly","termid":"FYPO:0007772"}],"references_by_uniquename":{},"uniquename":"SJAG_03891:allele-1"}


id: SJAG_03891:allele-2
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"E308V","gene":{"name":"alx1","product":"ESCRT-III regulator Alx1","uniquename":"SJAG_03891"},"genes_by_uniquename":{},"genotypes":[],"name":"rim20E308V","phenotypes":[],"references_by_uniquename":{},"uniquename":"SJAG_03891:allele-2"}


id: SJAG_03892:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"vps4","product":"AAA family ATPase Vps4","uniquename":"SJAG_03892"},"genes_by_uniquename":{},"genotypes":[{"display_name":"nur1delta vps4delta","display_uniquename":"nur1delta__vps4delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01050:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_03892:allele-1","expression":"Null"}]}]},{"display_name":"vps4delta","display_uniquename":"vps4delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03892:allele-1","expression":"Null"}]}]}],"name":"vps4delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000138","FYPO:0001985","FYPO:0005447","FYPO:0000002"],"is_obsolete":false,"name":"abnormal protein localization to heterochromatin","termid":"FYPO:0004718"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":7,"interesting_parent_ids":["FYPO:0001320","FYPO:0005447","FYPO:0000138","FYPO:0000002","FYPO:0001985"],"is_obsolete":false,"name":"abnormally clustered protein localization to nuclear envelope","termid":"FYPO:0007771"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":1,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985","FYPO:0001320","FYPO:0000138","FYPO:0005447"],"is_obsolete":false,"name":"abolished protein localization to heterochromatin during vegetative growth","termid":"FYPO:0001132"},{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":5,"interesting_parent_ids":["FYPO:0001985","FYPO:0000002","FYPO:0001320","FYPO:0005447"],"is_obsolete":false,"name":"delayed onset of mitotic nuclear membrane reassembly","termid":"FYPO:0007772"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0005447","FYPO:0001985","FYPO:0001320","FYPO:0000138","FYPO:0000002"],"is_obsolete":false,"name":"increased telomere tethering at nuclear periphery during mitosis","termid":"FYPO:0007770"},{"cv_name":"fission_yeast_phenotype","gene_count":9,"genotype_count":9,"interesting_parent_ids":["FYPO:0000139","FYPO:0005447","FYPO:0000003","FYPO:0002058","FYPO:0002057","FYPO:0001985","FYPO:0002060"],"is_obsolete":false,"name":"slow vegetative cell population growth","termid":"FYPO:0001234"}],"references_by_uniquename":{},"uniquename":"SJAG_03892:allele-1"}


id: SJAG_03892:allele-2
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"E233Q","gene":{"name":"vps4","product":"AAA family ATPase Vps4","uniquename":"SJAG_03892"},"genes_by_uniquename":{},"genotypes":[{"display_name":"Vps4-EQ(E233Q aa)","display_uniquename":"Vps4-EQ-E233Q-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03892:allele-2","expression":"Not assayed or wild type"}]}]}],"name":"Vps4-EQ","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":7,"interesting_parent_ids":["FYPO:0001320","FYPO:0005447","FYPO:0000138","FYPO:0000002","FYPO:0001985"],"is_obsolete":false,"name":"abnormally clustered protein localization to nuclear envelope","termid":"FYPO:0007771"}],"references_by_uniquename":{},"uniquename":"SJAG_03892:allele-2"}


id: SJAG_03892:allele-3
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"I307T L327S","gene":{"name":"vps4","product":"AAA family ATPase Vps4","uniquename":"SJAG_03892"},"genes_by_uniquename":{},"genotypes":[{"display_name":"cmp7delta vps4-ts(I307T L327S aa)","display_uniquename":"cmp7delta__vps4-ts-I307T_L327S-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_05207:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_03892:allele-3","expression":"Not assayed or wild type"}]}]},{"display_name":"vps4-ts(I307T L327S aa)","display_uniquename":"vps4-ts-I307T_L327S-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03892:allele-3","expression":"Not assayed or wild type"}]}]}],"name":"vps4-ts","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":7,"interesting_parent_ids":["FYPO:0001320","FYPO:0005447","FYPO:0000138","FYPO:0000002","FYPO:0001985"],"is_obsolete":false,"name":"abnormally clustered protein localization to nuclear envelope","termid":"FYPO:0007771"}],"references_by_uniquename":{},"uniquename":"SJAG_03892:allele-3"}


id: SJAG_03916:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"chp1","product":"heterochromatin (HP1) family chromodomain protein Chp1","uniquename":"SJAG_03916"},"genes_by_uniquename":{},"genotypes":[{"display_name":"chp1delta","display_uniquename":"chp1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03916:allele-1","expression":"Null"}]}]}],"name":"chp1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":9,"genotype_count":9,"interesting_parent_ids":["FYPO:0002059","FYPO:0002057","FYPO:0000003"],"is_obsolete":false,"name":"inviable vegetative cell population","termid":"FYPO:0002061"}],"references_by_uniquename":{},"uniquename":"SJAG_03916:allele-1"}


id: SJAG_03920:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"sdh3","product":"succinate dehydrogenase (ubiquinone) cytochrome b subunit","uniquename":"SJAG_03920"},"genes_by_uniquename":{},"genotypes":[{"display_name":"sdh3delta","display_uniquename":"sdh3delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03920:allele-1","expression":"Null"}]}]}],"name":"sdh3delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":31,"genotype_count":36,"interesting_parent_ids":["FYPO:0000003","FYPO:0002057"],"is_obsolete":false,"name":"viable cell population","termid":"FYPO:0002058"}],"references_by_uniquename":{},"uniquename":"SJAG_03920:allele-1"}


id: SJAG_03940:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"nem2","product":"serine/threonine protein phosphatase (Nem1-Spo7 complex) regulatory subunit","uniquename":"SJAG_03940"},"genes_by_uniquename":{},"genotypes":[{"display_name":"nem2delta","display_uniquename":"nem2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03940:allele-1","expression":"Null"}]}]}],"name":"nem2delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0002274","FYPO:0000002","FYPO:0005447","FYPO:0001985","FYPO:0002494","FYPO:0001320"],"is_obsolete":false,"name":"decreased protein dephosphorylation during vegetative growth","termid":"FYPO:0005957"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":5,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985","FYPO:0005447","FYPO:0001320"],"is_obsolete":false,"name":"excess endoplasmic reticulum membrane present","termid":"FYPO:0001784"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":6,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0000002","FYPO:0002403","FYPO:0001320"],"is_obsolete":false,"name":"excess nuclear envelope present","termid":"FYPO:0001556"}],"references_by_uniquename":{},"uniquename":"SJAG_03940:allele-1"}


id: SJAG_04009:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"ste11","product":"DNA-binding transcription factor Ste11","uniquename":"SJAG_04009"},"genes_by_uniquename":{},"genotypes":[{"display_name":"ste11delta","display_uniquename":"ste11delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04009:allele-1","expression":"Null"}]}]}],"name":"ste11delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":10,"genotype_count":10,"interesting_parent_ids":["FYPO:0001985","FYPO:0000301","FYPO:0005447"],"is_obsolete":false,"name":"sterile","secondary_identifiers":["FYPO:0000638"],"termid":"FYPO:0000280"},{"cv_name":"fission_yeast_phenotype","gene_count":23,"genotype_count":24,"interesting_parent_ids":["FYPO:0000002","FYPO:0000004"],"is_obsolete":false,"name":"viable cell","termid":"FYPO:0000124"}],"references_by_uniquename":{},"uniquename":"SJAG_04009:allele-1"}


id: SJAG_04061:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"arb2","product":"argonaute binding protein 2","uniquename":"SJAG_04061"},"genes_by_uniquename":{},"genotypes":[{"display_name":"arb2delta","display_uniquename":"arb2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04061:allele-1","expression":"Null"}]}]}],"name":"arb2delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":9,"genotype_count":9,"interesting_parent_ids":["FYPO:0002059","FYPO:0002057","FYPO:0000003"],"is_obsolete":false,"name":"inviable vegetative cell population","termid":"FYPO:0002061"}],"references_by_uniquename":{},"uniquename":"SJAG_04061:allele-1"}


id: SJAG_04152:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"ndi1","product":"internal mitochondrial NADH dehydrogenase (ubiquinone) Ndi1","uniquename":"SJAG_04152"},"genes_by_uniquename":{},"genotypes":[],"name":"ndi1delta","phenotypes":[],"references_by_uniquename":{},"uniquename":"SJAG_04152:allele-1"}


id: SJAG_04280:allele-1
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"T582G","gene":{"name":"wee1","product":"M phase inhibitor protein kinase Wee1","uniquename":"SJAG_04280"},"genes_by_uniquename":{},"genotypes":[{"display_name":"rga4delta wee1-as8(T582G aa)","display_uniquename":"rga4delta__wee1-as8-T582G-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04522:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_04280:allele-1","expression":"Not assayed or wild type"}]}]},{"display_name":"rga6delta wee1-as8(T582G aa)","display_uniquename":"rga6delta__wee1-as8-T582G-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00028:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_04280:allele-1","expression":"Not assayed or wild type"}]}]},{"display_name":"wee1-as8(T582G aa)","display_uniquename":"wee1-as8-T582G-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04280:allele-1","expression":"Not assayed or wild type"}]}]}],"name":"wee1-as8","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0001320","FYPO:0001985","FYPO:0005568","FYPO:0004803","FYPO:0000002","FYPO:0005447"],"is_obsolete":false,"name":"abnormal establishment or maintenance of actin cytoskeleton polarity during vegetative growth","termid":"FYPO:0000198"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0000138","FYPO:0005447","FYPO:0001985","FYPO:0000002","FYPO:0001320"],"is_obsolete":false,"name":"abnormally monopolar protein localization to cell tip","termid":"FYPO:0005503"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0001320","FYPO:0000002","FYPO:0001985","FYPO:0005447"],"is_obsolete":false,"name":"abolished geometry scaling","termid":"FYPO:0007783"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0005447","FYPO:0000002","FYPO:0001320","FYPO:0001985","FYPO:0000138"],"is_obsolete":false,"name":"increased protein localization to non-growing cell tip","termid":"FYPO:0002870"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0001320","FYPO:0000631","FYPO:0005447","FYPO:0000138","FYPO:0004803","FYPO:0001985","FYPO:0000002","FYPO:0000032"],"is_obsolete":false,"name":"mislocalized actomyosin contractile ring","termid":"FYPO:0001369"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":4,"interesting_parent_ids":["FYPO:0001985","FYPO:0000004","FYPO:0000002","FYPO:0000005","FYPO:0001320"],"is_obsolete":false,"name":"viable small vegetative cell","termid":"FYPO:0000648"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0000004","FYPO:0000005","FYPO:0000002","FYPO:0001985","FYPO:0001320"],"is_obsolete":false,"name":"viable thin vegetative cell","termid":"FYPO:0001877"}],"references_by_uniquename":{},"uniquename":"SJAG_04280:allele-1"}


id: SJAG_04280:allele-2
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"G788E","gene":{"name":"wee1","product":"M phase inhibitor protein kinase Wee1","uniquename":"SJAG_04280"},"genes_by_uniquename":{},"genotypes":[{"display_name":"rga4delta wee1-G788E(aa)","display_uniquename":"rga4delta__wee1-G788E-G788E-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04522:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_04280:allele-2","expression":"Not assayed or wild type"}]}]},{"display_name":"wee1-G788E(aa)","display_uniquename":"wee1-G788E-G788E-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04280:allele-2","expression":"Not assayed or wild type"}]}]}],"name":"wee1-G788E","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":3,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985"],"is_obsolete":false,"name":"mislocalized septum","termid":"FYPO:0004293"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":4,"interesting_parent_ids":["FYPO:0001985","FYPO:0000004","FYPO:0000002","FYPO:0000005","FYPO:0001320"],"is_obsolete":false,"name":"viable small vegetative cell","termid":"FYPO:0000648"}],"references_by_uniquename":{},"uniquename":"SJAG_04280:allele-2"}


id: SJAG_04280:allele-3
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"wee1","product":"M phase inhibitor protein kinase Wee1","uniquename":"SJAG_04280"},"genes_by_uniquename":{},"genotypes":[],"name":"wee1delta","phenotypes":[],"references_by_uniquename":{},"uniquename":"SJAG_04280:allele-3"}


id: SJAG_04284:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"nup61","product":"nucleoporin Nup61","uniquename":"SJAG_04284"},"genes_by_uniquename":{},"genotypes":[{"display_name":"nup61delta","display_uniquename":"nup61delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04284:allele-1","expression":"Null"}]}]}],"name":"nup61delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":2,"interesting_parent_ids":["FYPO:0000002","FYPO:0000631","FYPO:0001985","FYPO:0005447","FYPO:0001320"],"is_obsolete":false,"name":"abnormal cell cycle arrest in mitotic anaphase","termid":"FYPO:0000619"},{"cv_name":"fission_yeast_phenotype","gene_count":11,"genotype_count":16,"interesting_parent_ids":["FYPO:0000002","FYPO:0005447","FYPO:0001320","FYPO:0001985"],"is_obsolete":false,"name":"abnormal nuclear envelope organization","termid":"FYPO:0000815"}],"references_by_uniquename":{},"uniquename":"SJAG_04284:allele-1"}


id: SJAG_04287:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"cds1","product":"DNA replication checkpoint kinase Cds1","uniquename":"SJAG_04287"},"genes_by_uniquename":{},"genotypes":[{"display_name":"cds1delta","display_uniquename":"cds1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04287:allele-1","expression":"Null"}]}]}],"name":"cds1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":7,"genotype_count":6,"interesting_parent_ids":["FYPO:0000257"],"is_obsolete":false,"name":"normal hyphal growth","termid":"FYPO:0007790"},{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":6,"interesting_parent_ids":["FYPO:0000003","FYPO:0005447","FYPO:0001985","FYPO:0002683","FYPO:0000139"],"is_obsolete":false,"name":"sensitive to hydroxyurea","termid":"FYPO:0000088"}],"references_by_uniquename":{},"uniquename":"SJAG_04287:allele-1"}


id: SJAG_04312:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"pka1","product":"cAMP-dependent protein kinase catalytic subunit Pka1","uniquename":"SJAG_04312"},"genes_by_uniquename":{},"genotypes":[{"display_name":"pka1delta","display_uniquename":"pka1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04312:allele-1","expression":"Null"}]}]}],"name":"pka1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":25,"genotype_count":31,"interesting_parent_ids":["FYPO:0000002"],"is_obsolete":false,"name":"abnormal hyphal growth","termid":"FYPO:0007773"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0000005","FYPO:0001985","FYPO:0001320","FYPO:0000002"],"is_obsolete":false,"name":"elongated vegetative cell","termid":"FYPO:0001122"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0002057","FYPO:0001985","FYPO:0000003"],"is_obsolete":false,"name":"loss of viability in stationary phase","termid":"FYPO:0000245"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0005447","FYPO:0001985","FYPO:0000003","FYPO:0000139","FYPO:0002683"],"is_obsolete":false,"name":"sensitive to caffeine during vegetative growth","termid":"FYPO:0000097"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0001985","FYPO:0002683","FYPO:0000139","FYPO:0005447","FYPO:0000003"],"is_obsolete":false,"name":"sensitive to hydrogen peroxide","termid":"FYPO:0000087"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000003","FYPO:0001985","FYPO:0002683","FYPO:0000139","FYPO:0005447"],"is_obsolete":false,"name":"sensitive to potassium chloride","termid":"FYPO:0001214"}],"references_by_uniquename":{},"uniquename":"SJAG_04312:allele-1"}


id: SJAG_04332:allele-1
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"I30T,T572P","gene":{"name":"cdc25","product":"protein tyrosine phosphatase (M phase inducer) Cdc25","uniquename":"SJAG_04332"},"genes_by_uniquename":{},"genotypes":[{"display_name":"cdc25-D9(I30T,T572P aa)","display_uniquename":"cdc25-D9-I30T,T572P-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04332:allele-1","expression":"Not assayed or wild type"}]}]}],"name":"cdc25-D9","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0001320","FYPO:0000002","FYPO:0000004","FYPO:0000631","FYPO:0001985","FYPO:0005447","FYPO:0000005"],"is_obsolete":false,"name":"inviable elongated mononucleate cell with cell cycle arrest at mitotic G2/M phase transition","termid":"FYPO:0007701"}],"references_by_uniquename":{},"uniquename":"SJAG_04332:allele-1"}


id: SJAG_04349:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"vps28","product":"ESCRT I complex subunit Vps28","uniquename":"SJAG_04349"},"genes_by_uniquename":{},"genotypes":[{"display_name":"vps28delta","display_uniquename":"vps28delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04349:allele-1","expression":"Null"}]}]}],"name":"vps28delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0000138","FYPO:0000002"],"is_obsolete":false,"name":"abnormal protein complex localization","termid":"FYPO:0001440"}],"references_by_uniquename":{},"uniquename":"SJAG_04349:allele-1"}


id: SJAG_04464:allele-1
data:
  {"allele_type":"amino_acid_mutation","alleles_by_uniquename":{},"description":"R152C","gene":{"name":"pim1","product":"RCC1 family Ran GEF","uniquename":"SJAG_04464"},"genes_by_uniquename":{},"genotypes":[{"display_name":"cut3-T19E(aa) pim1-R152C(aa)","display_uniquename":"cut3-T19E-cut3-T19E-amino_acid_mutation-expression-not_assayed_or_wild_type__pim1-R152C-R152C-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00871:allele-1","expression":"Not assayed or wild type"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_04464:allele-1","expression":"Not assayed or wild type"}]}]},{"display_name":"pim1-R152C(aa)","display_uniquename":"pim1-R152C-R152C-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04464:allele-1","expression":"Not assayed or wild type"}]}]}],"name":"pim1-R152C","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":2,"interesting_parent_ids":["FYPO:0000002","FYPO:0000631","FYPO:0001985","FYPO:0005447","FYPO:0001320"],"is_obsolete":false,"name":"abnormal cell cycle arrest in mitotic anaphase","termid":"FYPO:0000619"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0000631","FYPO:0000002"],"is_obsolete":false,"name":"abnormal mitotic cell cycle arrest with condensed chromosomes","termid":"FYPO:0003738"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0000139","FYPO:0002057","FYPO:0000003","FYPO:0002058","FYPO:0001985","FYPO:0002060","FYPO:0005447"],"is_obsolete":false,"name":"decreased cell population growth at high temperature","secondary_identifiers":["FYPO:0000247"],"termid":"FYPO:0000082"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0000004","FYPO:0001320","FYPO:0000002"],"is_obsolete":false,"name":"inviable vegetative cell","termid":"FYPO:0001489"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0005447","FYPO:0000004","FYPO:0000002","FYPO:0001985","FYPO:0001320","FYPO:0002403"],"is_obsolete":false,"name":"inviable vegetative cell with fragmented nuclear envelope during mitotic telophase","termid":"FYPO:0003976"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":4,"interesting_parent_ids":["FYPO:0002057","FYPO:0001985","FYPO:0000003"],"is_obsolete":false,"name":"loss of viability at high temperature","termid":"FYPO:0001387"}],"references_by_uniquename":{},"synonyms":[{"name":"Inferred CDK-phospho-mimic mutation","reference":"PMID:28954740","type":"exact"}],"uniquename":"SJAG_04464:allele-1"}


id: SJAG_04489:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"did4","product":"ESCRT III complex subunit Did4","uniquename":"SJAG_04489"},"genes_by_uniquename":{},"genotypes":[{"display_name":"did4delta","display_uniquename":"did4delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04489:allele-1","expression":"Null"}]}]}],"name":"did4delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":13,"genotype_count":18,"interesting_parent_ids":["FYPO:0005447","FYPO:0000138","FYPO:0001985","FYPO:0000002"],"is_obsolete":false,"name":"abnormal protein localization","termid":"FYPO:0001370"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":7,"interesting_parent_ids":["FYPO:0001320","FYPO:0005447","FYPO:0000138","FYPO:0000002","FYPO:0001985"],"is_obsolete":false,"name":"abnormally clustered protein localization to nuclear envelope","termid":"FYPO:0007771"}],"references_by_uniquename":{},"uniquename":"SJAG_04489:allele-1"}


id: SJAG_04522:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"rga4","product":"RhoGAP Rga4","uniquename":"SJAG_04522"},"genes_by_uniquename":{},"genotypes":[{"display_name":"rga4delta","display_uniquename":"rga4delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04522:allele-1","expression":"Null"}]}]},{"display_name":"rga4delta rga6delta","display_uniquename":"rga4delta__rga6delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04522:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_00028:allele-1","expression":"Null"}]}]},{"display_name":"rga4delta wee1-as8(T582G aa)","display_uniquename":"rga4delta__wee1-as8-T582G-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04522:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_04280:allele-1","expression":"Not assayed or wild type"}]}]},{"display_name":"rga4delta wee1-G788E(aa)","display_uniquename":"rga4delta__wee1-G788E-G788E-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04522:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_04280:allele-2","expression":"Not assayed or wild type"}]}]}],"name":"rga4delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0001320","FYPO:0000002","FYPO:0001985","FYPO:0005447"],"is_obsolete":false,"name":"abolished geometry scaling","termid":"FYPO:0007783"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":2,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0000002","FYPO:0001320"],"is_obsolete":false,"name":"abolished polar cell growth","termid":"FYPO:0004105"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0001320","FYPO:0000631","FYPO:0005447","FYPO:0000138","FYPO:0004803","FYPO:0001985","FYPO:0000002","FYPO:0000032"],"is_obsolete":false,"name":"mislocalized actomyosin contractile ring","termid":"FYPO:0001369"},{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":3,"interesting_parent_ids":["FYPO:0000002","FYPO:0001985"],"is_obsolete":false,"name":"mislocalized septum","termid":"FYPO:0004293"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000005","FYPO:0000002","FYPO:0001985","FYPO:0001320","FYPO:0000004"],"is_obsolete":false,"name":"viable vegetative cell with increased cell diameter","termid":"FYPO:0006616"}],"references_by_uniquename":{},"uniquename":"SJAG_04522:allele-1"}


id: SJAG_04551:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"pmk1","product":"MAP kinase Pmk1","uniquename":"SJAG_04551"},"genes_by_uniquename":{},"genotypes":[{"display_name":"pck1delta pmk1delta","display_uniquename":"pck1delta__pmk1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01787:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_04551:allele-1","expression":"Null"}]}]},{"display_name":"pmk1delta","display_uniquename":"pmk1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04551:allele-1","expression":"Null"}]}]}],"name":"pmk1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":6,"genotype_count":9,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"decreased hyphal length","termid":"FYPO:0007776"},{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":8,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"decreased mycelial expansion","termid":"FYPO:0007780"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":4,"interesting_parent_ids":["FYPO:0001320","FYPO:0000002","FYPO:0001985"],"is_obsolete":false,"name":"multiseptate cell","termid":"FYPO:0004295"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":5,"interesting_parent_ids":["FYPO:0000139","FYPO:0005447","FYPO:0000003","FYPO:0001985","FYPO:0002682"],"is_obsolete":false,"name":"resistance to tacrolimus and magnesium chloride","termid":"FYPO:0007521"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000003","FYPO:0000139","FYPO:0002683","FYPO:0005447","FYPO:0001985"],"is_obsolete":false,"name":"sensitive to caspofungin","termid":"FYPO:0000079"},{"cv_name":"fission_yeast_phenotype","gene_count":23,"genotype_count":24,"interesting_parent_ids":["FYPO:0000002","FYPO:0000004"],"is_obsolete":false,"name":"viable cell","termid":"FYPO:0000124"}],"references_by_uniquename":{},"uniquename":"SJAG_04551:allele-1"}


id: SJAG_04551:allele-2
data:
  {"allele_type":"amino_acid_insertion","alleles_by_uniquename":{},"description":"82-NITCIYDLDIIINPYNFNEVYIYEE-105","gene":{"name":"pmk1","product":"MAP kinase Pmk1","uniquename":"SJAG_04551"},"genes_by_uniquename":{},"genotypes":[{"display_name":"Pmk1Sj(ins82-105)(82-NITCIYDLDIIINPYNFNEVYIYEE-105)","display_uniquename":"Pmk1Sj(ins82-105)-82-NITCIYDLDIIINPYNFNEVYIYEE-105-amino_acid_insertion-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04551:allele-2","expression":"Not assayed or wild type"}]}]}],"name":"Pmk1Sj(ins82-105)","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":6,"genotype_count":9,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"decreased hyphal length","termid":"FYPO:0007776"},{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":8,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"decreased mycelial expansion","termid":"FYPO:0007780"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0005447","FYPO:0000002","FYPO:0002274","FYPO:0001320","FYPO:0001985","FYPO:0002494"],"is_obsolete":false,"name":"decreased protein phosphorylation during vegetative growth","termid":"FYPO:0001838"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":5,"interesting_parent_ids":["FYPO:0000139","FYPO:0005447","FYPO:0000003","FYPO:0001985","FYPO:0002682"],"is_obsolete":false,"name":"resistance to tacrolimus and magnesium chloride","termid":"FYPO:0007521"}],"references_by_uniquename":{},"uniquename":"SJAG_04551:allele-2"}


id: SJAG_04551:allele-3
data:
  {"allele_type":"other","alleles_by_uniquename":{},"description":"Pmk1 from S. pombe","gene":{"name":"pmk1","product":"MAP kinase Pmk1","uniquename":"SJAG_04551"},"genes_by_uniquename":{},"genotypes":[{"display_name":"Pmk1Sp(Pmk1 from S. pombe)","display_uniquename":"Pmk1Sp-Pmk1_from_S._pombe-other-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04551:allele-3","expression":"Not assayed or wild type"}]}]}],"name":"Pmk1Sp","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":6,"genotype_count":9,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"decreased hyphal length","termid":"FYPO:0007776"},{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":8,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"decreased mycelial expansion","termid":"FYPO:0007780"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":5,"interesting_parent_ids":["FYPO:0000139","FYPO:0005447","FYPO:0000003","FYPO:0001985","FYPO:0002682"],"is_obsolete":false,"name":"resistance to tacrolimus and magnesium chloride","termid":"FYPO:0007521"}],"references_by_uniquename":{},"uniquename":"SJAG_04551:allele-3"}


id: SJAG_04551:allele-4
data:
  {"allele_type":"partial_amino_acid_deletion","alleles_by_uniquename":{},"description":"Pmk1 from S. pombe with deleted residues 82-105","gene":{"name":"pmk1","product":"MAP kinase Pmk1","uniquename":"SJAG_04551"},"genes_by_uniquename":{},"genotypes":[{"display_name":"Pmk1Sp(delta82-105)(Pmk1 from S. pombe with deleted residues 82-105 Δaa)","display_uniquename":"Pmk1Sp(delta82-105)-Pmk1_from_S._pombe_with_deleted_residues_82-105-partial_amino_acid_deletion-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04551:allele-4","expression":"Not assayed or wild type"}]}]}],"name":"Pmk1Sp(delta82-105)","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0002494","FYPO:0005447","FYPO:0002274","FYPO:0001985","FYPO:0000002"],"is_obsolete":false,"name":"abolished protein phosphorylation","termid":"FYPO:0002678"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0005447","FYPO:0001320","FYPO:0000002","FYPO:0002274","FYPO:0002494","FYPO:0001985"],"is_obsolete":false,"name":"abolished protein phosphorylation during vegetative growth","termid":"FYPO:0002033"},{"cv_name":"fission_yeast_phenotype","gene_count":6,"genotype_count":9,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"decreased hyphal length","termid":"FYPO:0007776"},{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":8,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"decreased mycelial expansion","termid":"FYPO:0007780"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":5,"interesting_parent_ids":["FYPO:0000139","FYPO:0005447","FYPO:0000003","FYPO:0001985","FYPO:0002682"],"is_obsolete":false,"name":"resistance to tacrolimus and magnesium chloride","termid":"FYPO:0007521"}],"references_by_uniquename":{},"uniquename":"SJAG_04551:allele-4"}


id: SJAG_04574:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"clr4","product":"histone lysine H3-K9 methyltransferase (Suv39) Clr4","uniquename":"SJAG_04574"},"genes_by_uniquename":{},"genotypes":[{"display_name":"clr4delta","display_uniquename":"clr4delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04574:allele-1","expression":"Null"}]}]},{"display_name":"clr4delta leo1delta","display_uniquename":"clr4delta__leo1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04574:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_03118:allele-1","expression":"Null"}]}]}],"name":"clr4delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":9,"genotype_count":9,"interesting_parent_ids":["FYPO:0002059","FYPO:0002057","FYPO:0000003"],"is_obsolete":false,"name":"inviable vegetative cell population","termid":"FYPO:0002061"}],"references_by_uniquename":{},"uniquename":"SJAG_04574:allele-1"}


id: SJAG_04671:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"mrc1","product":"claspin, Mrc1","uniquename":"SJAG_04671"},"genes_by_uniquename":{},"genotypes":[{"display_name":"mrc1delta","display_uniquename":"mrc1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04671:allele-1","expression":"Null"}]}]}],"name":"mrc1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":7,"genotype_count":6,"interesting_parent_ids":["FYPO:0000257"],"is_obsolete":false,"name":"normal hyphal growth","termid":"FYPO:0007790"},{"cv_name":"fission_yeast_phenotype","gene_count":7,"genotype_count":8,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0000139","FYPO:0002683","FYPO:0000003"],"is_obsolete":false,"name":"sensitive to camptothecin","termid":"FYPO:0000085"},{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":6,"interesting_parent_ids":["FYPO:0000003","FYPO:0005447","FYPO:0001985","FYPO:0002683","FYPO:0000139"],"is_obsolete":false,"name":"sensitive to hydroxyurea","termid":"FYPO:0000088"}],"references_by_uniquename":{},"uniquename":"SJAG_04671:allele-1"}


id: SJAG_04697:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"gms1","product":"Golgi UDP-galactose transmembrane transporter Gms1","uniquename":"SJAG_04697"},"genes_by_uniquename":{},"genotypes":[{"display_name":"gms1delta","display_uniquename":"gms1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04697:allele-1","expression":"Null"}]}]}],"name":"gms1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000002","FYPO:0000004"],"is_obsolete":false,"name":"cell lysis during hyphal growth phase","termid":"FYPO:0008029"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0000139","FYPO:0002057","FYPO:0000003","FYPO:0002058","FYPO:0001985","FYPO:0002060","FYPO:0005447"],"is_obsolete":false,"name":"decreased cell population growth at high temperature","secondary_identifiers":["FYPO:0000247"],"termid":"FYPO:0000082"},{"cv_name":"fission_yeast_phenotype","gene_count":6,"genotype_count":9,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"decreased hyphal length","termid":"FYPO:0007776"},{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":8,"interesting_parent_ids":["FYPO:0007773","FYPO:0000002"],"is_obsolete":false,"name":"decreased mycelial expansion","termid":"FYPO:0007780"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0002494","FYPO:0000002","FYPO:0001320","FYPO:0002274","FYPO:0001985","FYPO:0005447","FYPO:0003290","FYPO:0002946"],"is_obsolete":false,"name":"galactose absent from cell wall glycoprotein glycan","termid":"FYPO:0003727"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000003","FYPO:0005447","FYPO:0002683","FYPO:0001985","FYPO:0000139"],"is_obsolete":false,"name":"sensitive to hygromycin B","termid":"FYPO:0000106"}],"references_by_uniquename":{},"uniquename":"SJAG_04697:allele-1"}


id: SJAG_04703:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"for3","product":"formin For3","uniquename":"SJAG_04703"},"genes_by_uniquename":{},"genotypes":[{"display_name":"for3delta","display_uniquename":"for3delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04703:allele-1","expression":"Null"}]}]}],"name":"for3delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000002","FYPO:0001320","FYPO:0004803","FYPO:0001985","FYPO:0005447"],"is_obsolete":false,"name":"actin cables absent from cell","termid":"FYPO:0003315"},{"cv_name":"fission_yeast_phenotype","gene_count":13,"genotype_count":15,"interesting_parent_ids":["FYPO:0002058","FYPO:0005447","FYPO:0001985","FYPO:0002060","FYPO:0002057","FYPO:0000139","FYPO:0000003"],"is_obsolete":false,"name":"decreased vegetative cell population growth","termid":"FYPO:0001355"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0001985","FYPO:0000002","FYPO:0000005"],"is_obsolete":false,"name":"spherical cell","termid":"FYPO:0001956"}],"references_by_uniquename":{},"uniquename":"SJAG_04703:allele-1"}


id: SJAG_04777:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"win1","product":"MAP kinase kinase kinase Win1","uniquename":"SJAG_04777"},"genes_by_uniquename":{},"genotypes":[{"display_name":"win1delta","display_uniquename":"win1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04777:allele-1","expression":"Null"}]}]}],"name":"win1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":23,"genotype_count":24,"interesting_parent_ids":["FYPO:0000002","FYPO:0000004"],"is_obsolete":false,"name":"viable cell","termid":"FYPO:0000124"}],"references_by_uniquename":{},"uniquename":"SJAG_04777:allele-1"}


id: SJAG_04843:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"fum1","product":"fumarate hydratase","uniquename":"SJAG_04843"},"genes_by_uniquename":{},"genotypes":[{"display_name":"fum1delta","display_uniquename":"fum1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04843:allele-1","expression":"Null"}]}]}],"name":"fum1delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":31,"genotype_count":36,"interesting_parent_ids":["FYPO:0000003","FYPO:0002057"],"is_obsolete":false,"name":"viable cell population","termid":"FYPO:0002058"}],"references_by_uniquename":{},"uniquename":"SJAG_04843:allele-1"}


id: SJAG_04883:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"ptp4","product":"phosphatidate cytidylyltransferase Ptp4/Dgk1","uniquename":"SJAG_04883"},"genes_by_uniquename":{},"genotypes":[{"display_name":"ptp4delta","display_uniquename":"ptp4delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04883:allele-1","expression":"Null"}]}]}],"name":"ptp4delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0001312","FYPO:0000002","FYPO:0000257","FYPO:0001320"],"is_obsolete":false,"name":"normal mitosis","termid":"FYPO:0001007"}],"references_by_uniquename":{},"uniquename":"SJAG_04883:allele-1"}


id: SJAG_04912:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"vps25","product":"ESCRT II complex subunit Vps25","uniquename":"SJAG_04912"},"genes_by_uniquename":{},"genotypes":[{"display_name":"vps25delta","display_uniquename":"vps25delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04912:allele-1","expression":"Null"}]}]}],"name":"vps25delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":3,"interesting_parent_ids":["FYPO:0001985","FYPO:0005447","FYPO:0000138","FYPO:0000002"],"is_obsolete":false,"name":"abnormal protein complex localization","termid":"FYPO:0001440"}],"references_by_uniquename":{},"uniquename":"SJAG_04912:allele-1"}


id: SJAG_05207:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"cmp7","product":"ESCRT III complex subunit, nuclear envelope specific subunit Cmp7","uniquename":"SJAG_05207"},"genes_by_uniquename":{},"genotypes":[{"display_name":"cmp7delta","display_uniquename":"cmp7delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_05207:allele-1","expression":"Null"}]}]},{"display_name":"cmp7delta vps4-ts(I307T L327S aa)","display_uniquename":"cmp7delta__vps4-ts-I307T_L327S-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_05207:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_03892:allele-3","expression":"Not assayed or wild type"}]}]}],"name":"cmp7delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":4,"genotype_count":7,"interesting_parent_ids":["FYPO:0001320","FYPO:0005447","FYPO:0000138","FYPO:0000002","FYPO:0001985"],"is_obsolete":false,"name":"abnormally clustered protein localization to nuclear envelope","termid":"FYPO:0007771"},{"cv_name":"fission_yeast_phenotype","gene_count":3,"genotype_count":5,"interesting_parent_ids":["FYPO:0000139","FYPO:0002060","FYPO:0002057","FYPO:0001985","FYPO:0002058","FYPO:0005447","FYPO:0000003"],"is_obsolete":false,"name":"decreased cell population growth at low temperature","secondary_identifiers":["FYPO:0002175"],"termid":"FYPO:0000080"},{"cv_name":"fission_yeast_phenotype","gene_count":5,"genotype_count":5,"interesting_parent_ids":["FYPO:0001985","FYPO:0000002","FYPO:0001320","FYPO:0005447"],"is_obsolete":false,"name":"delayed onset of mitotic nuclear membrane reassembly","termid":"FYPO:0007772"},{"cv_name":"fission_yeast_phenotype","gene_count":1,"genotype_count":1,"interesting_parent_ids":["FYPO:0000139","FYPO:0002683","FYPO:0000003","FYPO:0005447","FYPO:0001985"],"is_obsolete":false,"name":"sensitive to leptomycin B","termid":"FYPO:0000714"},{"cv_name":"fission_yeast_phenotype","gene_count":9,"genotype_count":9,"interesting_parent_ids":["FYPO:0000139","FYPO:0005447","FYPO:0000003","FYPO:0002058","FYPO:0002057","FYPO:0001985","FYPO:0002060"],"is_obsolete":false,"name":"slow vegetative cell population growth","termid":"FYPO:0001234"}],"references_by_uniquename":{},"uniquename":"SJAG_05207:allele-1"}


id: SJAG_05242:allele-1
data:
  {"allele_type":"deletion","alleles_by_uniquename":{},"description":"deletion","gene":{"name":"wcs2","product":"blue light signalling transcription factor, zf-GATA type Wcs2","uniquename":"SJAG_05242"},"genes_by_uniquename":{},"genotypes":[{"display_name":"wcs2delta","display_uniquename":"wcs2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_05242:allele-1","expression":"Null"}]}]}],"name":"wcs2delta","phenotypes":[{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000298","FYPO:0000002"],"is_obsolete":false,"name":"abnormal blue light response","termid":"FYPO:0008031"},{"cv_name":"fission_yeast_phenotype","gene_count":2,"genotype_count":2,"interesting_parent_ids":["FYPO:0000003"],"is_obsolete":false,"name":"decreased sexual agglutination in response to blue light","termid":"FYPO:0008046"}],"references_by_uniquename":{},"uniquename":"SJAG_05242:allele-1"}


# refs

id: PMID:37233261
data:
  {"abstract":"Gene targeting is a commonly used method to reveal the function of genes. Although it is an attractive tool for molecular studies, it can frequently be a challenge because its efficiency can be low and it requires the screening of a large number of transformants. Generally, these problems originate from the elevated level of ectopic integration caused by non-homologous DNA end joining (NHEJ). To eliminate this problem, NHEJ-related genes are frequently deleted or disrupted. Although these manipulations can improve gene targeting, the phenotype of the mutant strains raised the question of whether mutations have side effects. The aim of this study was to disrupt the  lig4  gene in the dimorphic fission yeast,  S. japonicus , and investigate the phenotypic changes of the mutant strain. The mutant cells have shown various phenotypic changes, such as increased sporulation on complete medium, decreased hyphal growth, faster chronological aging, and higher sensitivity to heat shock, UV light, and caffeine. In addition, higher flocculation capacity has been observed, especially at lower sugar concentrations. These changes were supported by transcriptional profiling. Many genes belonging to metabolic and transport processes, cell division, or signaling had altered mRNA levels compared to the control strain. Although the disruption improved the gene targeting, we assume that the  lig4  inactivation can cause unexpected physiological side effects, and we have to be very careful with the manipulations of the NHEJ-related genes. To reveal the exact mechanisms behind these changes, further investigations are required.","alleles_by_uniquename":{"SJAG_02527:allele-1":{"allele_type":"disruption","description":"RNA sequencing revealed that only the 5' end of the disrupted allele showed low RNA expression and coverage","gene_uniquename":"SJAG_02527","name":"lig4::KanMX6","uniquename":"SJAG_02527:allele-1"}},"annotation_curators":[{"annotation_count":7,"community_curator":true,"file_name":null,"file_type":null,"name":"Ida Miklos","orcid":"0000-0002-7074-4019"}],"annotation_details":{},"annotation_file_curators":[],"approved_date":"2023-08-08","authors":"Acs-Szabo L, Papp LA, Takacs S, Miklos I","authors_abbrev":"Acs-Szabo L et al.","canto_added_date":"2023-05-27 00:40:04","canto_annotation_status":"APPROVED","canto_approved_date":"2023-08-08 20:07:33","canto_approver_orcid":"0000-0002-8138-6851","canto_curator_name":"Ida Miklos","canto_curator_role":"community","canto_first_approved_date":"2023-08-08 20:07:33","canto_session_key":"c74bdd0a67b132f8","canto_session_submitted_date":"2023-08-08 20:07:15","canto_triage_status":"Curatable","citation":"J Fungi (Basel) 2023 May 10;9(5)","cv_annotations":{"single_locus_phenotype":[{"annotations":[115328],"is_not":false,"summary":null,"term":"FYPO:0007776"},{"annotations":[115330],"is_not":false,"summary":null,"term":"FYPO:0009111"},{"annotations":[115327],"is_not":false,"summary":null,"term":"FYPO:0000588"},{"annotations":[115333],"is_not":false,"summary":null,"term":"FYPO:0005705"},{"annotations":[115331],"is_not":false,"summary":null,"term":"FYPO:0005231"},{"annotations":[115329],"is_not":false,"summary":null,"term":"FYPO:0000097"},{"annotations":[115332],"is_not":false,"summary":null,"term":"FYPO:0001408"}]},"doi":"10.3390/jof9050550","gene_count":1,"genes_by_uniquename":{"SJAG_02527":null},"genotypes_by_uniquename":{"lig4::KanMX6-RNA_sequencing_revealed_that_only_the_5'_end_of_the_disrupted_allele_showed_low_RNA_expression_and_coverage-disruption-expression-knockdown":{"display_name":"lig4::KanMX6(RNA sequencing revealed that only the 5' end of the disrupted allele showed low RNA expression and coverage)[Knockdown]","display_uniquename":"lig4::KanMX6-RNA_sequencing_revealed_that_only_the_5'_end_of_the_disrupted_allele_showed_low_RNA_expression_and_coverage-disruption-expression-knockdown","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02527:allele-1","expression":"Knockdown"}]}]}},"ltp_gene_count":1,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2023","pubmed_entrez_date":"2023-05-26","pubmed_publication_date":"10 May 2023","terms_by_termid":{"FYECO:0000005":null,"FYECO:0000081":null,"FYECO:0000117":null,"FYECO:0000126":null,"FYECO:0000137":null,"FYECO:0000315":null,"FYPO:0000097":null,"FYPO:0000588":null,"FYPO:0001408":null,"FYPO:0005231":null,"FYPO:0005705":null,"FYPO:0007776":null,"FYPO:0009111":null},"title":"Disruption of the  Schizosaccharomyces japonicus lig4  Disturbs Several Cellular Processes and Leads to a Pleiotropic Phenotype.","uniquename":"PMID:37233261"}


id: PMID:12795405
data:
  {"abstract":"Spectrin, a component of the membrane skeleton in erythrocytes and other animal cells, has also been identified in plant and fungal cells. However, its postulated role, i.e., the maintenance of shape and elasticity of the plasma membrane, is probably not exerted in walled cells. To study spectrin in these cells, we chose yeasts because of a high morphological variability of their life cycle. The localization of spectrin in the cells and protoplasts of Saccharomyces cerevisiae and Schizosaccharomyces japonicus var. versatilis was detected by immunoblotting, indirect immunofluorescence, and immunogold electron microscopy techniques with the use of anti-chicken and anti-human erythrocyte spectrin antibodies. A protein band of 220-240 kDa and some bands of lower relative mass were detected in cell and protoplast extracts of both yeast strains. Spectrin-like proteins were revealed by fluorescence microscopy at cell surfaces and in vacuolar membranes. Immunogold-labelling showed spectrin-like proteins in the plasma membrane, endoplasmic reticulum, vacuoles, nuclei, vesicles, mitochondria, and cell walls. The topology of spectrin was not affected by actin depolymerization with Latrunculin B nor was it changed in either act1-1 or cdc42 mutants, under restrictive conditions. Under osmotic stress, both spectrin and actin were delocalized and appeared in the form of large clusters in the cytoplasm. It is concluded that a protein cross-reacting with spectrin antibodies is present in fission and budding yeasts. Generally, it is located in the proximity of the plasma membrane and other intracellular membranes, probably as a part of the membrane skeleton. No evidence of its relationship to either actin or growth zones of the cell can be provided.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Slaninová I, Holubárová A, Svoboda A","authors_abbrev":"Slaninová I et al.","canto_added_date":"2021-05-10 08:46:37","canto_curator_role":"JaponicusDB","canto_triage_status":"Method or reagent","citation":"Can J Microbiol 2003 Mar;49(3):189-96","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2003","pubmed_entrez_date":"2003-06-11","pubmed_publication_date":"Mar 2003","terms_by_termid":{},"title":"Immunodetection of spectrin-like proteins in yeasts.","uniquename":"PMID:12795405"}


id: PMID:34353908
data:
  {"abstract":"Biosynthesis of sterols, which are key constituents of canonical eukaryotic membranes, requires molecular oxygen. Anaerobic protists and deep-branching anaerobic fungi are the only eukaryotes in which a mechanism for sterol-independent growth has been elucidated. In these organisms, tetrahymanol, formed through oxygen-independent cyclization of squalene by a squalene-tetrahymanol cyclase, acts as a sterol surrogate. This study confirms an early report [C. J. E. A. Bulder,  Antonie Van Leeuwenhoek , 37, 353-358 (1971)] that  Schizosaccharomyces japonicus  is exceptional among yeasts in growing anaerobically on synthetic media lacking sterols and unsaturated fatty acids. Mass spectrometry of lipid fractions of anaerobically grown  Sch. japonicus  showed the presence of hopanoids, a class of cyclic triterpenoids not previously detected in yeasts, including hop-22(29)-ene, hop-17(21)-ene, hop-21(22)-ene, and hopan-22-ol. A putative gene in  Sch. japonicus  showed high similarity to bacterial squalene-hopene cyclase (SHC) genes and in particular to those of  Acetobacter  species. No orthologs of the putative  Sch. japonicus  SHC were found in other yeast species. Expression of the  Sch. japonicus  SHC gene ( Sjshc1 ) in  Saccharomyces cerevisiae  enabled hopanoid synthesis and stimulated anaerobic growth in sterol-free media, thus indicating that one or more of the hopanoids produced by SjShc1 could at least partially replace sterols. Use of hopanoids as sterol surrogates represents a previously unknown adaptation of eukaryotic cells to anaerobic growth. The fast anaerobic growth of  Sch. japonicus  in sterol-free media is an interesting trait for developing robust fungal cell factories for application in anaerobic industrial processes.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Bouwknegt J, Wiersma SJ, Ortiz-Merino RA, Doornenbal ESR, Buitenhuis P, Giera M, Müller C, Pronk JT","authors_abbrev":"Bouwknegt J et al.","canto_added_date":"2023-05-04 00:40:04","canto_curator_role":"JaponicusDB","canto_triage_status":"Cell composition or WT feature","citation":"Proc Natl Acad Sci U S A 2021 Aug 10;118(32)","cv_annotations":{},"doi":"10.1073/pnas.2105225118","gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2021","pubmed_entrez_date":"2021-08-06","pubmed_publication_date":"10 Aug 2021","terms_by_termid":{},"title":"A squalene-hopene cyclase in  Schizosaccharomyces japonicus  represents a eukaryotic adaptation to sterol-limited anaerobic environments.","uniquename":"PMID:34353908"}


id: GO_REF:0000070
data:
  {"abstract":"We have created a standard template for classes describing the transmembrane transporter activity a chemical entity (ChEBI) as molecular function. This includes variants for secondary active transmembrane transporter activity (GO:0015291), uptake transmembrane transporter activity (GO:0015563), and ATPase activity, coupled to transmembrane movement of substances (GO:0042626). The underlying equivalence axiom template is \"G and 'transports or maintains localization of' some X\",  where the genus G is either GO:0022857 (transmembrane transporter activity), GO:0015291, GO:0015563, or GO:0042626 depending on the variant. The variable X is a chemical entity (CHEBI:24431). The approach to combine GO and ChEBI has been described in the following publication: PMID:23895341.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"GO ontology editors","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Representation of transmembrane transporter activity as molecular function in the Gene Ontology","uniquename":"GO_REF:0000070"}


id: GO_REF:0000083
data:
  {"abstract":"We have created a standard template for classes describing the morphogenesis of a plant structure as a biological process. The underlying equivalence axiom template is \"'anatomical structure morphogenesis' and 'results in morphogenesis of' some P\", where P is a plant anatomical entity (PO:0025131).","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"GO ontology editors","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Representation of plant morphogenesis as biological process in the Gene Ontology","uniquename":"GO_REF:0000083"}


id: GO_REF:0000113
data:
  {"abstract":"The TFClass (http://tfclass.bioinf.med.uni-goettingen.de/index.jsf) database provides a comprehensive classification of mammalian DNA binding transcription factors (DbTFs) based on their DNA binding domains (DBDs) (PMID:29087517). TFClass classifies mammalian DbTFs by a five-level classification in which the four highest levels represent groups defined by structural and sequence similarities (superclass, class, family, subfamily, and genera) (more details at http://www.edgar-wingender.de/TFClass_schema.html). This classification is based on the combination of background knowledge of the molecular structural features of DBDs (PMID:9340487, PMID:23427989) and phylogenetic trees constructed via multiple sequence alignment with hierarchical clustering of manually validated DBDs and/or full-length protein sequences retrieved from UniProt (PMID:23427989, PMID:23180794, PMID:23427989). ","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Marcio Luis Acencio (1), George Georghiou (2), Sandra Orchard (2), Liv Thommensen (1), Martin Kuiper (1) and Astrid Lægreid (1). (1) Norwegian University of Science and Technology (NTNU), Trondheim, Norway; (2) European Bioinformatics Institute (EBI), Hinxton, Cambridgeshire, United Kingdom","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Gene Ontology annotation of human sequence-specific DNA binding transcription factors (DbTFs) based on the TFClass database","uniquename":"GO_REF:0000113"}


id: PMID:34198697
data:
  {"abstract":"Mitogen activated protein kinase (MAPK) signaling pathways execute essential functions in eukaryotic organisms by transducing extracellular stimuli into adaptive cellular responses. In the fission yeast model  Schizosaccharomyces pombe  the cell integrity pathway (CIP) and its core effector, MAPK Pmk1, play a key role during regulation of cell integrity, cytokinesis, and ionic homeostasis.  Schizosaccharomyces japonicus , another fission yeast species, shows remarkable differences with respect to  S. pombe , including a robust yeast to hyphae dimorphism in response to environmental changes. We show that the CIP MAPK module architecture and its upstream regulators, PKC orthologs Pck1 and Pck2, are conserved in both fission yeast species. However, some of  S. pombe 's CIP-related functions, such as cytokinetic control and response to glucose availability, are regulated differently in  S. japonicus.  Moreover, Pck1 and Pck2 antagonistically regulate  S. japonicus  hyphal differentiation through fine-tuning of Pmk1 activity. Chimeric MAPK-swapping experiments revealed that  S. japonicus  Pmk1 is fully functional in  S. pombe , whereas  S. pombe  Pmk1 shows a limited ability to execute CIP functions and promote  S. japonicus  mycelial development. Our findings also suggest that a modified N-lobe domain secondary structure within  S. japonicus  Pmk1 has a major influence on the CIP signaling features of this evolutionarily diverged fission yeast.","alleles_by_uniquename":{"SJAG_00610:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_00610","name":"pck2delta","uniquename":"SJAG_00610:allele-1"},"SJAG_01787:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_01787","name":"pck1delta","uniquename":"SJAG_01787:allele-1"},"SJAG_02592:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_02592","name":"sty1delta","uniquename":"SJAG_02592:allele-1"},"SJAG_04551:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_04551","name":"pmk1delta","uniquename":"SJAG_04551:allele-1"},"SJAG_04551:allele-2":{"allele_type":"amino_acid_insertion","description":"82-NITCIYDLDIIINPYNFNEVYIYEE-105","gene_uniquename":"SJAG_04551","name":"Pmk1Sj(ins82-105)","uniquename":"SJAG_04551:allele-2"},"SJAG_04551:allele-3":{"allele_type":"other","description":"Pmk1 from S. pombe","gene_uniquename":"SJAG_04551","name":"Pmk1Sp","uniquename":"SJAG_04551:allele-3"},"SJAG_04551:allele-4":{"allele_type":"partial_amino_acid_deletion","description":"Pmk1 from S. pombe with deleted residues 82-105","gene_uniquename":"SJAG_04551","name":"Pmk1Sp(delta82-105)","uniquename":"SJAG_04551:allele-4"}},"annotation_curators":[{"annotation_count":34,"community_curator":true,"file_name":null,"file_type":null,"name":"Elisa Gomez Gil","orcid":null}],"annotation_details":{},"annotation_file_curators":[],"approved_date":"2021-09-14","authors":"Gómez-Gil E, Franco A, Vázquez-Marín B, Prieto-Ruiz F, Pérez-Díaz A, Vicente-Soler J, Madrid M, Soto T, Cansado J","authors_abbrev":"Gómez-Gil E et al.","canto_added_date":"2021-09-14 16:45:58","canto_annotation_status":"APPROVED","canto_approved_date":"2021-09-14 21:32:21","canto_approver_orcid":"0000-0002-8138-6851","canto_curator_name":"Elisa Gomez Gil","canto_curator_role":"community","canto_first_approved_date":"2021-09-14 21:32:21","canto_session_key":"0ba747685c452996","canto_session_submitted_date":"2021-09-14 17:59:01","canto_triage_status":"Curatable","citation":"J Fungi (Basel) 2021 Jun 14;7(6)","cv_annotations":{"PSI-MOD":[{"annotations":[115215,115216,115217],"is_not":false,"summary":null,"term":"MOD:00696"}],"cellular_component":[{"annotations":[115214],"is_not":false,"summary":null,"term":"GO:0005737"},{"annotations":[115213],"is_not":false,"summary":null,"term":"GO:0005634"}],"multi_locus_phenotype":[{"annotations":[115231],"is_not":false,"summary":null,"term":"FYPO:0007780"}],"single_locus_phenotype":[{"annotations":[115229,115237],"is_not":false,"summary":null,"term":"FYPO:0002678"},{"annotations":[115236],"is_not":false,"summary":null,"term":"FYPO:0002033"},{"annotations":[115218,115230,115234,115239,115242],"is_not":false,"summary":null,"term":"FYPO:0007776"},{"annotations":[115219,115235,115240,115243],"is_not":false,"summary":null,"term":"FYPO:0007780"},{"annotations":[115228,115232],"is_not":false,"summary":null,"term":"FYPO:0001838"},{"annotations":[115223],"is_not":false,"summary":null,"term":"FYPO:0007775"},{"annotations":[115224],"is_not":false,"summary":null,"term":"FYPO:0007779"},{"annotations":[115225],"is_not":false,"summary":null,"term":"FYPO:0002680"},{"annotations":[115222],"is_not":false,"summary":null,"term":"FYPO:0001038"},{"annotations":[115210],"is_not":false,"summary":null,"term":"FYPO:0004295"},{"annotations":[115220],"is_not":false,"summary":null,"term":"FYPO:0005152"},{"annotations":[115212,115227,115233,115238,115241],"is_not":false,"summary":null,"term":"FYPO:0007521"},{"annotations":[115211,115226],"is_not":false,"summary":null,"term":"FYPO:0000079"},{"annotations":[115221],"is_not":false,"summary":null,"term":"FYPO:0007522"}]},"doi":"10.3390/jof7060482","gene_count":4,"genes_by_uniquename":{"SJAG_00610":null,"SJAG_01787":null,"SJAG_02592":null,"SJAG_04551":null},"genotypes_by_uniquename":{"Pmk1Sj(ins82-105)-82-NITCIYDLDIIINPYNFNEVYIYEE-105-amino_acid_insertion-expression-not_assayed_or_wild_type":{"display_name":"Pmk1Sj(ins82-105)(82-NITCIYDLDIIINPYNFNEVYIYEE-105)","display_uniquename":"Pmk1Sj(ins82-105)-82-NITCIYDLDIIINPYNFNEVYIYEE-105-amino_acid_insertion-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04551:allele-2","expression":"Not assayed or wild type"}]}]},"Pmk1Sp(delta82-105)-Pmk1_from_S._pombe_with_deleted_residues_82-105-partial_amino_acid_deletion-expression-not_assayed_or_wild_type":{"display_name":"Pmk1Sp(delta82-105)(Pmk1 from S. pombe with deleted residues 82-105 Δaa)","display_uniquename":"Pmk1Sp(delta82-105)-Pmk1_from_S._pombe_with_deleted_residues_82-105-partial_amino_acid_deletion-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04551:allele-4","expression":"Not assayed or wild type"}]}]},"Pmk1Sp-Pmk1_from_S._pombe-other-expression-not_assayed_or_wild_type":{"display_name":"Pmk1Sp(Pmk1 from S. pombe)","display_uniquename":"Pmk1Sp-Pmk1_from_S._pombe-other-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04551:allele-3","expression":"Not assayed or wild type"}]}]},"pck1delta":{"display_name":"pck1delta","display_uniquename":"pck1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01787:allele-1","expression":"Null"}]}]},"pck1delta__pmk1delta":{"display_name":"pck1delta pmk1delta","display_uniquename":"pck1delta__pmk1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01787:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_04551:allele-1","expression":"Null"}]}]},"pck2delta":{"display_name":"pck2delta","display_uniquename":"pck2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00610:allele-1","expression":"Null"}]}]},"pmk1delta":{"display_name":"pmk1delta","display_uniquename":"pmk1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04551:allele-1","expression":"Null"}]}]},"sty1delta":{"display_name":"sty1delta","display_uniquename":"sty1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02592:allele-1","expression":"Null"}]}]}},"ltp_gene_count":4,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2021","pubmed_entrez_date":"2021-07-02","pubmed_publication_date":"14 Jun 2021","terms_by_termid":{"FYECO:0000207":null,"FYECO:0000314":null,"FYPO:0000079":null,"FYPO:0001038":null,"FYPO:0001838":null,"FYPO:0002033":null,"FYPO:0002678":null,"FYPO:0002680":null,"FYPO:0004295":null,"FYPO:0005152":null,"FYPO:0007521":null,"FYPO:0007522":null,"FYPO:0007775":null,"FYPO:0007776":null,"FYPO:0007779":null,"FYPO:0007780":null,"FYPO_EXT:0000001":null,"FYPO_EXT:0000002":null,"GO:0005634":null,"GO:0005737":null,"GO:0036460":null,"GO:0042149":null,"GO:0071475":null,"GO:0072690":null,"MOD:00696":null},"title":"Specific Functional Features of the Cell Integrity MAP Kinase Pathway in the Dimorphic Fission Yeast  Schizosaccharomyces japonicus .","uniquename":"PMID:34198697"}


id: GO_REF:0000025
data:
  {"abstract":"Genes in prokaryotic organisms are often arranged in operons. Genes in an operon are all transcribed into one mRNA. Generally the genes in the operons code for proteins that all have related functions. For example, they may be the steps in a biochemical pathway, or they may be the subunits of a protein complex. Often the genes in operons shared between organisms are syntenic; that is, the same genes are in the same order in the operon in different species. When assessing sequence-comparison-based evidence during the process of manual annotation of a genome, it is often the case that some of the genes in the operon will have strong sequence-based evidence while others will have weak evidence. If seen alone, not in the presence of an operon, the weak evidence in question may not be sufficient to make a functional annotation. However, in the presence of an operon in which there is strong evidence for some of the genes, the very presence of the gene in the operon is a strong indication that the gene shares in the process carried out by the operon. If the putative function is one expected to exist for the process in question and particularly if that function has been observed in the same operon in another species, then the annotation should be made. This type of evidence is inferred from the context of the gene in an operon, and therefore the evidence code is IGC \"inferred from genomic context.\"","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Michelle Gwinn, TIGR curators","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Operon structure as IGC evidence","uniquename":"GO_REF:0000025"}


id: PMID:7166567
data:
  {"abstract":"The fine structure of ascospore formation in the fission yeasts Schizosaccharomyces pombe and Schizosaccharomyces japonicus var. japonicus was studied by serial thin-sectioning and electron microscopy. The morphogenetic events were almost the same in both species. Ascospore development was initiated by the formation of the forespore membrane on the cytoplasmic side of the differentiated nucleus-associated organelle (NAO) in the interval between meiosis I and II in S. pombe, or during the post-meiotic nuclear division in S. japonicus, and the process proceeded almost synchronously through the two or four nuclei in the ascus. The forespore membrane developed by fusion of the cytoplasmic vesicles and this was clearly demonstrated in S. japonicus where the behaviour of vesicles involved in the forespore membrane development could be traced as they were marked by the presence of electron-dense granules. The staining technique, by phosphotungustic acid--chromic acid (PTA-CA) after treatment with periodic acid, was used to attempt to elucidate the origin and the nature of the forespore membrane. The method specific to plasmalemma-type membranes stained both ascus and ascospore plasmalemmas; the forespore membrane was not stained at first but developed the same affinity for stain as the plasma membrane in the course of ascospore development. The results suggest that the forespore membrane did not come directly from the ascus plasma membrane, but from another membrane system such as the endoplasmic reticulum. Spore wall material was deposited in the space between the inner and outer leaflets of the forespore membrane.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Tanaka K, Hirata A","authors_abbrev":"Tanaka K et al.","canto_added_date":"2021-05-10 08:46:37","canto_curator_role":"JaponicusDB","canto_triage_status":"Cell composition or WT feature","citation":"J Cell Sci 1982 Aug;56:263-79","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"1982","pubmed_entrez_date":"1982-08-01","pubmed_publication_date":"Aug 1982","terms_by_termid":{},"title":"Ascospore development in the fission yeasts Schizosaccharomyces pombe and S. japonicus.","uniquename":"PMID:7166567"}


id: PMID:21802294
data:
  {"abstract":"Eukaryotes segregate chromosomes in \"open\" or \"closed\" mitosis, depending on whether their nuclear envelopes (NEs) break down or remain intact. Here we show that the control of the nuclear surface area may determine the choice between these two modes. The dividing nucleus does not expand its surface in the fission yeast Schizosaccharomyces japonicus, confining the mitotic spindle and causing it to buckle. The NE ruptures in anaphase, releasing the compressive stress and allowing chromosome segregation. Blocking the NE expansion in the related species Schizosaccharomyces pombe that undergoes closed mitosis induces spindle buckling and collapse in the absence of an intrinsic NE rupture mechanism. We propose that scaling considerations could have shaped the evolution of eukaryotic mitosis by necessitating either nuclear surface expansion or the NE breakdown.","alleles_by_uniquename":{"SJAG_01330:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_01330","name":"mad2delta","uniquename":"SJAG_01330:allele-1"},"SJAG_01745:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_01745","name":"lem2delta","uniquename":"SJAG_01745:allele-1"}},"annotation_curators":[{"annotation_count":16,"community_curator":false,"file_name":null,"file_type":null,"name":"Snezhana Oliferenko","orcid":"0000-0002-8138-6851"},{"annotation_count":1,"community_curator":false,"file_name":null,"file_type":null,"name":"Val Wood","orcid":"0000-0001-6330-7526"}],"annotation_details":{},"annotation_file_curators":[],"approved_date":"2021-07-05","authors":"Yam C, He Y, Zhang D, Chiam KH, Oliferenko S","authors_abbrev":"Yam C et al.","canto_added_date":"2021-05-10 02:38:30","canto_annotation_status":"APPROVED","canto_approved_date":"2021-09-01 07:44:20","canto_approver_orcid":"0000-0001-6330-7526","canto_curator_name":"Snezhana Oliferenko","canto_curator_role":"JaponicusDB","canto_first_approved_date":"2021-07-05 11:58:24","canto_session_key":"ec486c9b0898d3c4","canto_session_submitted_date":"2021-07-05 12:18:55","canto_triage_status":"Curatable","citation":"Curr Biol 2011 Aug 09;21(15):1314-9","cv_annotations":{"biological_process":[{"annotations":[115171],"is_not":false,"summary":null,"term":"GO:0101024"}],"cellular_component":[{"annotations":[115160],"is_not":false,"summary":null,"term":"GO:0000785"},{"annotations":[115164,115165],"is_not":false,"summary":null,"term":"GO:0005783"},{"annotations":[115166],"is_not":false,"summary":null,"term":"GO:0098826"},{"annotations":[115159],"is_not":false,"summary":null,"term":"GO:0000776"},{"annotations":[115156],"is_not":false,"summary":null,"term":"GO:0005874"},{"annotations":[115161,115162,115163,115167],"is_not":false,"summary":null,"term":"GO:0005643"},{"annotations":[115157,115158],"is_not":false,"summary":null,"term":"GO:0005730"}],"single_locus_phenotype":[{"annotations":[115169],"is_not":false,"summary":null,"term":"FYPO:0003779"},{"annotations":[115168],"is_not":false,"summary":null,"term":"FYPO:0004318"},{"annotations":[115170],"is_not":false,"summary":null,"term":"FYPO:0007772"}]},"doi":"10.1016/j.cub.2011.06.052","gene_count":13,"genes_by_uniquename":{"SJAG_00175":null,"SJAG_00471":null,"SJAG_00684":null,"SJAG_00943":null,"SJAG_01006":null,"SJAG_01330":null,"SJAG_01374":null,"SJAG_01460":null,"SJAG_01745":null,"SJAG_02509":null,"SJAG_02830":null,"SJAG_03299":null,"SJAG_03603":null},"genotypes_by_uniquename":{"lem2delta":{"display_name":"lem2delta","display_uniquename":"lem2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01745:allele-1","expression":"Null"}]}]},"mad2delta":{"display_name":"mad2delta","display_uniquename":"mad2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01330:allele-1","expression":"Null"}]}]}},"ltp_gene_count":13,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2011","pubmed_entrez_date":"2011-08-02","pubmed_publication_date":"09 Aug 2011","terms_by_termid":{"FYECO:0000162":null,"FYPO:0003779":null,"FYPO:0004318":null,"FYPO:0007772":null,"FYPO_EXT:0000001":null,"FYPO_EXT:0000002":null,"GO:0000776":null,"GO:0000785":null,"GO:0005643":null,"GO:0005730":null,"GO:0005783":null,"GO:0005874":null,"GO:0098826":null,"GO:0101024":null},"title":"Divergent strategies for controlling the nuclear membrane satisfy geometric constraints during nuclear division.","uniquename":"PMID:21802294"}


id: PMID:9884240
data:
  {"abstract":"Fission yeast cells grow by extension at the ends (poles) and divide by transverse fission. It has previously been reported that Schizosaccharomyces japonicus var. japonicus can switch to unipolar, filamentous growth. Here it is shown that the yeast-to-mycelium transition is a gradual process involving a changeover to unipolar growth associated with asymmetric divisions, the development of large polarly located vacuoles, the modifications of the actin and microtubular cytoskeleton and the repression of cell separation after division. High concentrations of glucose in the medium or supplementation of the medium with caffeine or cAMP support the bipolar yeast phase, inhibit the transition to the mycelial phase and induce the conversion of hyphae to yeasts. These effects suggest that cAMP may be involved in the regulation of dimorphism. Temperatures below 18 degrees C or over 35 degrees C are restrictive for the mycelial phase and provoke a return to yeast phase.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"approved_date":"2021-10-07","authors":"Sipiczki M, Takeo K, Grallert A","authors_abbrev":"Sipiczki M et al.","canto_added_date":"2021-05-10 08:46:37","canto_annotation_status":"APPROVED","canto_approved_date":"2021-10-07 12:31:51","canto_approver_orcid":"0000-0002-8138-6851","canto_curator_role":"JaponicusDB","canto_first_approved_date":"2021-10-07 12:31:51","canto_session_key":"5ac54d09f220d215","canto_session_submitted_date":"2021-09-30 09:01:47","canto_triage_status":"Curatable","citation":"Microbiology (Reading) 1998 Dec;144 ( Pt 12):3475-3485","cv_annotations":{},"doi":"10.1099/00221287-144-12-3475","gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"1998","pubmed_entrez_date":"1999-01-12","pubmed_publication_date":"Dec 1998","terms_by_termid":{},"title":"Growth polarity transitions in a dimorphic fission yeast.","uniquename":"PMID:9884240"}


id: GO_REF:0000111
data:
  {"abstract":"The Gene Ontology Consortium uses the IC (Inferred by Curator; ECO:0000305) evidence code when assignment of a GO term cannot be supported by direct experimental or sequence-based evidence, but can, based on a curator’s biological knowledge, be reasonably inferred from existing GO annotations to the same gene/gene product.  Use of the IC evidence code with GO_REF:0000111 indicates that a curator inferred the GO term based on at least one supporting annotation with an 'Inferred from Sequence Similarity' (ISS; ECO:0000250) evidence code.  Note that additional supporting annotations may be experimentally evidenced. When using GO_REF:0000111, the 'with/from' field must contain all GO identifiers used as supporting annotations.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"TBD","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference","uniquename":"GO_REF:0000111"}


id: GO_REF:0000008
data:
  {"abstract":"The sequence conservation that permits the establishment of orthology between mouse and rat or mouse and human genes is a strong predictor of the conservation of function for the gene product across these species. Therefore, in instances where a mouse gene product has not been functionally characterized, but its human or rat orthologs have, Mouse Genome Informatics (MGI) curators append the GO terms associated with the orthologous gene(s) to the mouse gene. Only those GO terms assigned by experimental determination to the ortholog of the mouse gene will be adopted by MGI. GO terms that are assigned to the ortholog of the mouse gene computationally (i.e. IEA), will not be transferred to the mouse ortholog. The evidence code represented by this citation is Inferred by Sequence Orthology (ISO).","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Mouse Genome Informatics scientific curators","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Gene Ontology annotation by the MGI curatorial staff, curated orthology","uniquename":"GO_REF:0000008"}


id: GO_REF:0000085
data:
  {"abstract":"We have created a standard template for classes describing the apoptotic process for a cell type as a biological process. The underlying equivalence axiom template is \"'apoptotic process' and 'occurs in' some C\", where C is a native cell (CL:0000003).","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"GO ontology editors","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Representation of cell apoptotic process as biological process in the Gene Ontology","uniquename":"GO_REF:0000085"}


id: PMID:30664646
data:
  {"abstract":"Cells of a specific cell type may divide within a certain size range. Yet, functionally optimal cellular organization is typically maintained across different cell sizes, a phenomenon known as scaling. The mechanisms underlying scaling and its physiological significance remain elusive. Here we approach this problem by interfering with scaling in the rod-shaped fission yeast Schizosaccharomyces japonicus that relies on cellular geometry cues to position the division site. We show that S. japonicus uses the Cdc42 polarity module to adjust its geometry to changes in the cell size. When scaling is prevented resulting in abnormal cellular length-to-width aspect ratio, cells exhibit severe division site placement defects. We further show that despite the generally accepted view, a similar scaling phenomenon can occur in the sister species, Schizosaccharomyces pombe. Our results demonstrate that scaling is required for normal cell function and delineate possible rules for cellular geometry maintenance in populations of proliferating cells.","alleles_by_uniquename":{"SJAG_00028:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_00028","name":"rga6delta","uniquename":"SJAG_00028:allele-1"},"SJAG_00514:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_00514","name":"scd1delta","uniquename":"SJAG_00514:allele-1"},"SJAG_03048:allele-1":{"allele_type":"amino_acid_mutation","description":"G146D","gene_uniquename":"SJAG_03048","name":"cdc2-1w","uniquename":"SJAG_03048:allele-1"},"SJAG_03581:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_03581","name":"gef1delta","uniquename":"SJAG_03581:allele-1"},"SJAG_04280:allele-1":{"allele_type":"amino_acid_mutation","description":"T582G","gene_uniquename":"SJAG_04280","name":"wee1-as8","uniquename":"SJAG_04280:allele-1"},"SJAG_04280:allele-2":{"allele_type":"amino_acid_mutation","description":"G788E","gene_uniquename":"SJAG_04280","name":"wee1-G788E","uniquename":"SJAG_04280:allele-2"},"SJAG_04522:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_04522","name":"rga4delta","uniquename":"SJAG_04522:allele-1"}},"annotation_curators":[{"annotation_count":3,"community_curator":false,"file_name":null,"file_type":null,"name":"Val Wood","orcid":"0000-0001-6330-7526"},{"annotation_count":22,"community_curator":true,"file_name":null,"file_type":null,"name":"Ying Gu","orcid":null}],"annotation_details":{},"annotation_file_curators":[],"approved_date":"2021-07-02","authors":"Gu Y, Oliferenko S","authors_abbrev":"Gu Y et al.","canto_added_date":"2021-05-10 02:37:31","canto_annotation_status":"APPROVED","canto_approved_date":"2021-09-01 07:53:28","canto_approver_orcid":"0000-0001-6330-7526","canto_curator_name":"Ying Gu","canto_curator_role":"community","canto_first_approved_date":"2021-07-02 12:32:27","canto_session_key":"0922e2a5229110bd","canto_session_submitted_date":"2021-06-30 22:18:13","canto_triage_status":"Curatable","citation":"Nat Commun 2019 01 21;10(1):268","cv_annotations":{"biological_process":[{"annotations":[115322],"is_not":false,"summary":null,"term":"GO:0061171"},{"annotations":[115323,115324],"is_not":false,"summary":null,"term":"GO:0008360"}],"multi_locus_phenotype":[{"annotations":[115310,115315],"is_not":false,"summary":null,"term":"FYPO:0007783"},{"annotations":[115321],"is_not":false,"summary":null,"term":"FYPO:0004105"},{"annotations":[115312],"is_not":false,"summary":null,"term":"FYPO:0001369"},{"annotations":[115314],"is_not":false,"summary":null,"term":"FYPO:0004293"}],"single_locus_phenotype":[{"annotations":[115316],"is_not":false,"summary":null,"term":"FYPO:0000198"},{"annotations":[115317,115318,115320],"is_not":false,"summary":null,"term":"FYPO:0005503"},{"annotations":[115311],"is_not":false,"summary":null,"term":"FYPO:0007783"},{"annotations":[115305],"is_not":false,"summary":null,"term":"FYPO:0004105"},{"annotations":[115319],"is_not":false,"summary":null,"term":"FYPO:0002870"},{"annotations":[115313],"is_not":false,"summary":null,"term":"FYPO:0001369"},{"annotations":[115306],"is_not":false,"summary":null,"term":"FYPO:0001234"},{"annotations":[115300,115301,115304],"is_not":false,"summary":null,"term":"FYPO:0000648"},{"annotations":[115302,115303,115309],"is_not":false,"summary":null,"term":"FYPO:0001877"},{"annotations":[115307,115308],"is_not":false,"summary":null,"term":"FYPO:0006616"}]},"doi":"10.1038/s41467-018-08218-2","gene_count":8,"genes_by_uniquename":{"SJAG_00028":null,"SJAG_00030":null,"SJAG_00514":null,"SJAG_01794":null,"SJAG_03048":null,"SJAG_03581":null,"SJAG_04280":null,"SJAG_04522":null},"genotypes_by_uniquename":{"cdc2-1w-G146D-amino_acid_mutation-expression-not_assayed_or_wild_type":{"display_name":"cdc2-1w(G146D aa)","display_uniquename":"cdc2-1w-G146D-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03048:allele-1","expression":"Not assayed or wild type"}]}]},"gef1delta":{"display_name":"gef1delta","display_uniquename":"gef1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03581:allele-1","expression":"Null"}]}]},"rga4delta":{"display_name":"rga4delta","display_uniquename":"rga4delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04522:allele-1","expression":"Null"}]}]},"rga4delta__rga6delta":{"display_name":"rga4delta rga6delta","display_uniquename":"rga4delta__rga6delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04522:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_00028:allele-1","expression":"Null"}]}]},"rga4delta__wee1-G788E-G788E-amino_acid_mutation-expression-not_assayed_or_wild_type":{"display_name":"rga4delta wee1-G788E(aa)","display_uniquename":"rga4delta__wee1-G788E-G788E-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04522:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_04280:allele-2","expression":"Not assayed or wild type"}]}]},"rga4delta__wee1-as8-T582G-amino_acid_mutation-expression-not_assayed_or_wild_type":{"display_name":"rga4delta wee1-as8(T582G aa)","display_uniquename":"rga4delta__wee1-as8-T582G-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04522:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_04280:allele-1","expression":"Not assayed or wild type"}]}]},"rga6delta":{"display_name":"rga6delta","display_uniquename":"rga6delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00028:allele-1","expression":"Null"}]}]},"rga6delta__wee1-as8-T582G-amino_acid_mutation-expression-not_assayed_or_wild_type":{"display_name":"rga6delta wee1-as8(T582G aa)","display_uniquename":"rga6delta__wee1-as8-T582G-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00028:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_04280:allele-1","expression":"Not assayed or wild type"}]}]},"scd1delta":{"display_name":"scd1delta","display_uniquename":"scd1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00514:allele-1","expression":"Null"}]}]},"wee1-G788E-G788E-amino_acid_mutation-expression-not_assayed_or_wild_type":{"display_name":"wee1-G788E(aa)","display_uniquename":"wee1-G788E-G788E-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04280:allele-2","expression":"Not assayed or wild type"}]}]},"wee1-as8-T582G-amino_acid_mutation-expression-not_assayed_or_wild_type":{"display_name":"wee1-as8(T582G aa)","display_uniquename":"wee1-as8-T582G-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04280:allele-1","expression":"Not assayed or wild type"}]}]}},"ltp_gene_count":6,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2019","pubmed_entrez_date":"2019-01-22","pubmed_publication_date":"21 01 2019","terms_by_termid":{"FYECO:0000004":null,"FYECO:0000005":null,"FYECO:0000006":null,"FYECO:0000103":null,"FYECO:0000126":null,"FYECO:0000137":null,"FYECO:0000338":null,"FYPO:0000198":null,"FYPO:0000648":null,"FYPO:0001234":null,"FYPO:0001369":null,"FYPO:0001877":null,"FYPO:0002870":null,"FYPO:0004105":null,"FYPO:0004293":null,"FYPO:0005503":null,"FYPO:0006616":null,"FYPO:0007783":null,"FYPO_EXT:0000001":null,"FYPO_EXT:0000002":null,"GO:0008360":null,"GO:0061171":null},"title":"Cellular geometry scaling ensures robust division site positioning.","uniquename":"PMID:30664646"}


id: PMID:11921097
data:
  {"abstract":"The dimorphic fission yeast Schizosaccharomyces japonicus has proved to be an excellent experimental model for the investigation of the eukaryotic cell. Here we show that it has a haplontic life cycle, in which the diploid phase is confined to the zygote. To make it amenable to genetic and molecular analysis, we generated genetic markers and cloned a genomic sequence which acts as ars when integrated into a plasmid. Diploids suitable for testing complementation and recombination between markers can be formed by protoplast fusion. The complementation tests and the recombination frequencies determined in octads of spores identified 28 non-allelic groups (genes) of mutations of the auxotrophic and mycelium-negative mutants. Two groups of linked markers were also identified. The cloned fragment, which expresses ars activity, encodes a putative amino acid sequence highly similar to a conserved domain of proteins Cut1 (Schizosaccharomyces pombe), BimB (Aspergillus nidulans) and Esp1 (Saccharomyces cerevisiae).","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Bozsik A, Szilagyi Z, Benko Z, Sipiczki M","authors_abbrev":"Bozsik A et al.","canto_added_date":"2021-05-10 08:46:37","canto_curator_role":"JaponicusDB","canto_triage_status":"Method or reagent","citation":"Yeast 2002 Apr;19(6):485-98","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2002","pubmed_entrez_date":"2002-03-29","pubmed_publication_date":"Apr 2002","terms_by_termid":{},"title":"Marker construction and cloning of a cut1-like sequence with ARS activity in the fission yeast Schizosaccharomyces japonicus.","uniquename":"PMID:11921097"}


id: PMID:33232355
data:
  {"abstract":"Coenzyme Q (CoQ, ubiquinone) is an essential component of the electron transport system in aerobic organisms. Human type CoQ10, which has 10 units of isoprene in its quinone structure, is especially valuable as a food supplement. Therefore, studying the biosynthesis of CoQ10 is important not only for increasing metabolic knowledge, but also for improving biotechnological production. Herein, we show that Schizosaccharomyces pombe utilizes p-aminobenzoate (PABA) in addition to p-hydroxybenzoate (PHB) as a precursor for CoQ10 synthesis. We explored compounds that affect the synthesis of CoQ10 and found benzoic acid (Bz) at >5 μg/mL inhibited CoQ biosynthesis without accumulation of apparent CoQ intermediates. This inhibition was counteracted by incubation with a 10-fold lower amount of PABA or PHB. Overexpression of PHB-polyprenyl transferase encoded by ppt1 (coq2) also overcame the inhibition of CoQ biosynthesis by Bz. Inhibition by Bz was efficient in S. pombe and Schizosaccharomyces japonicus, but less so in Saccharomyces cerevisiae, Aureobasidium pullulans, and Escherichia coli. Bz also inhibited a S. pombe ppt1 (coq2) deletion strain expressing human COQ2, and this strain also utilized PABA as a precursor of CoQ10. Thus, Bz is likely to inhibit prenylation reactions involving PHB or PABA catalyzed by Coq2.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Nishida I, Yanai R, Matsuo Y, Kaino T, Kawamukai M","authors_abbrev":"Nishida I et al.","canto_added_date":"2021-05-10 08:46:37","canto_curator_role":"JaponicusDB","canto_triage_status":"Cell composition or WT feature","citation":"PLoS One 2020;15(11):e0242616","cv_annotations":{},"doi":"10.1371/journal.pone.0242616","gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2020","pubmed_entrez_date":"2020-11-24","pubmed_publication_date":"2020","terms_by_termid":{},"title":"Benzoic acid inhibits Coenzyme Q biosynthesis in Schizosaccharomyces pombe.","uniquename":"PMID:33232355"}


id: GO_REF:0000012
data:
  {"abstract":"Pairwise alignments are generated by taking two sequences and aligning them so that the maximum number of amino acids in each protein match, or are similar to, each other. Tools such as BLAST work by comparing a protein-of-interest individually with every protein in a database of known protein sequences and retaining only those matches with a high probability of being significant. Basic BLAST generates local alignments between proteins for regions of high similarity. Other pairwise alignment tools attempt to generate global (full-length) protein alignments. A tool called Blast_Extend_repraze (BER, http://ber.sourceforge.net) has some benefits over basic BLAST. Input into the BER tool includes the underlying DNA sequence for each protein as well as 300 nucleotides upstream and downstream of the predicted boundaries of the protein coding sequence. This allows annotators to see the DNA sequence that underlies the query protein as part of the alignment. In addition, the BER tool is able to look for continuation of regions of similarity through frameshifts and in-frame stop codons.  If such regions are found the alignment is continued. BER searches are done in a two-step process: step one is a BLAST search against a non-redundant protein database, significant BLAST hits are stored in a mini-database for each query protein; step two is a modified Smith-Waterman alignment between the query and the proteins in its mini-database. In order to assess whether a given BER alignment is good enough to assert that the query shares the function of the match protein, one must look at a several factors. First of all, the match protein must itself be experimentally characterized in order to avoid transitive annotation errors. In addition, any residues or secondary structures known to be important for function in the match protein must be conserved in the query. The alignment should be visually inspected to look for any areas of lesser quality that might indicate the two proteins do not share the same function. Although it is impossible to set cutoff values for percent identity and length of match that will apply for every alignment, there are some guidelines. In general at least 40% identity that extends over the full lengths of both proteins is required in order to even consider functional equivalence. However, this percentage is highly dependent on the length and complexity of the proteins. 40% identity between two proteins 500 amino acids long is much more significant that 40% identity between two proteins that are only 100 amino acids long. Therefore, the annotator's experience and knowledge of what is considered significant for the organism and protein family in question is very important. Some sets of proteins are much more highly conserved than others and therefore tolerances for percent identity may have to be adjusted. Finally, the alignment must be considered in the context of what else is known about the query protein and the organism as a whole.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Michelle Gwinn, TIGR curators","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Pairwise alignment (TIGR)","uniquename":"GO_REF:0000012"}


id: GO_REF:0000109
data:
  {"abstract":"Trypanosomes are exquisitely structured cells in which protein localisation can be extremely informative for likely function. TrypTag is a project using expression of N- and C-terminal mNeonGreen fusion proteins from the endogenous loci to determine the subcellular localisation of every gene in the Trypanosoma brucei genome. GO Cellular Component terms are manually assigned by curators studying fluorescence microscope images of the resulting cells labelled with mNeonGreen fusion proteins. As trypanosomes are a pathogenic basal eukaryote, this will indicate likely function of both highly conserved eukaryote genes and parasite-specific genes. Resource URL: http://www.tryptag.org Protein subcellular localisation images can be viewed on the Tryptag website, e.g. http://www.tryptag.org?id=Tb927.8.1550","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"TrypTag","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Gene Ontology annotation based on curation of genome-wide subcellular localisation of proteins using fluorescent protein tagging in Trypanosoma brucei","uniquename":"GO_REF:0000109"}


id: PMID:23934997
data:
  {"abstract":"Both budding yeast Saccharomyces cerevisiae and fission yeast Schizosaccahromyces pombe have been very popular organisms used for biological research with eukaryotes for many decades. Judging from the fission yeast Schizosaccharomyces japonicus DNA sequence determined 2 years ago, this species is evolutionarily very much unrelated to the commonly used yeasts for research. Indicating evolutionary divergence, the S. japonicus makes 8-spored asci and mitosis occurs with a partial breakdown of nuclear membrane whereas the other yeasts make 4-spored asci and cells divide without nuclear breakdown. The commonly used yeast species exhibit a generation time between 1.5 and 2.0 hr, and their genetic cross takes a period of more than 7 working d. As described here, a generation time of only 63 min and meiotic analysis completed in just 2.5 d, the S. japonicus fission yeast is predicted to become a choice organism for future research on the biology of eukaryotes. ","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Klar AJ","authors_abbrev":"Klar AJ","canto_added_date":"2021-05-10 08:46:37","canto_curator_role":"JaponicusDB","canto_triage_status":"Review or comment","citation":"G3 (Bethesda) 2013 Oct 03;3(10):1869-73","cv_annotations":{},"doi":"10.1534/g3.113.007187","gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2013","pubmed_entrez_date":"2013-08-13","pubmed_publication_date":"03 Oct 2013","terms_by_termid":{},"title":"Schizosaccharomyces japonicus yeast poised to become a favorite experimental organism for eukaryotic research.","uniquename":"PMID:23934997"}


id: GO_REF:0000079
data:
  {"abstract":"We have created a standard template for classes describing the assembly or disassembly of a cellular component as a biological process. The underlying equivalence axiom templates are \"GO:0022607 and 'results_in_assembly_of' some C\" (assembly) and \"GO:0022411 and 'results_in_disassembly_of' some C\" (disassembly), where C is a cellular component.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"GO ontology editors","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Representation of assembly or disassembly of a cell component as biological process in the Gene Ontology","uniquename":"GO_REF:0000079"}


id: PMID:31732056
data:
  {"abstract":"Vitis davidii Föex is widely planted in South China as a potential wine making grape. In this study, yeast communities during the spontaneous fermentations of two varieties of Vitis davidii Föex in Guizhou, China were investigated. Hanseniaspora uvarum, Pichia terricola, Saccharomyces cerevisiae/S. mikatae, and Schizosaccharomyces japonicus were the four main yeast species detected by culture-dependent and high-throughput sequencing approaches. However, the composition of minor yeast species was quite different as revealed by the two approaches. Ten yeast species including Debaryomyces hansenii, Rhodotorula mucilaginosa, Sporidiobolus paraoseus, Starmerella bacillaris, Zygoascus meyerae, etc. were detected by culture-dependent approaches, whereas the other five species were found by high-throughput sequencing analysis. S. japonicus was widely found during spontaneous fermentations and its concentrations were mostly higher than that of S. cerevisiae. The difference in grape varieties and fermentation conditions contributed to yeast diversity. The results of this study provide basic information on indigenous yeast diversity from Vitis davidii Föex in Guizhou, which would help to exploit the potential of non-Saccharomyces yeast species with good oenological attributes.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Wang C, Wu C, Qiu S","authors_abbrev":"Wang C et al.","canto_added_date":"2021-05-10 08:46:37","canto_curator_role":"JaponicusDB","canto_triage_status":"Biotech","citation":"Food Res Int 2019 12;126:108582","cv_annotations":{},"doi":"10.1016/j.foodres.2019.108582","gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2019","pubmed_entrez_date":"2019-11-17","pubmed_publication_date":"12 2019","terms_by_termid":{},"title":"Yeast diversity investigation of Vitis davidii Föex during spontaneous fermentations using culture-dependent and high-throughput sequencing approaches.","uniquename":"PMID:31732056"}


id: GO_REF:0000086
data:
  {"abstract":"We have created a standard template for classes describing the differentiation process for a cell type as a biological process. The underlying equivalence axiom template is \"GO:0030154 and 'results in acquisition of features of' some C\", where C is a native cell (CL:0000003).","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"GO ontology editors","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Representation of cell differentiation as biological process in the Gene Ontology","uniquename":"GO_REF:0000086"}


id: GO_REF:0000033
data:
  {"abstract":"The Phylogenetic ANnotation using Gene Ontology (PAN-GO) method annotates evolutionary trees from the PANTHER database with GO terms describing molecular function, biological process and cellular component. The GO terms are manually selected by a curator and used to annotate ancestral genes in the phylogenetic tree using the evidence code IBA (Inferred from Biological Ancestor). All supporting annotations must be based on experimental data from the scientific literature. The PAN-GO annotations are fully traceable from the data in the 'with/from' column of the annotation, which provides the PANTHER node ID (PTN) from which the annotation is derived, as well as all descendants sequences that support the annotation of the ancestral node. ","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Marc Feuermann, Huaiyu Mi, Pascale Gaudet, Dustin Ebert, Anushya Muruganujan, Paul 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id: PMID:25318672
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id: PMID:8529460
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  {"abstract":"Meiotic prophase in Schizosaccharomyces pombe is characterized by striking nuclear movements and the formation of linear elements along chromosomes instead of tripartite synaptonemal complexes. We analysed the organization of nuclei and microtubules in cells of fission yeasts undergoing sexual differentiation. S. japonicus var. versatilis and S. pombe cells were studied in parallel, taking advantage of the better cytology in S. versatilis. During conjugation, microtubules were directed towards the mating projection. These microtubules seem to lead the haploid nuclei together in the zygote by interaction with the spindle pole bodies at the nuclear periphery. After karyogamy, arrays of microtubules emanating from the spindle pole body of the diploid nucleus extended to both cell poles. The same differentiated microtubule configuration was elaborated upon induction of azygotic meiosis in S. pombe. The cyclic movements of the elongated nuclei between the cell poles is reflected by a dynamic and coordinated shortening and lengthening of the two microtubule arrays. When the nucleus was at a cell end, one array was short while the other bridged the whole cell length. Experiments with inhibitors showed that microtubules are required for karyogamy and for the elongated shape and movement of nuclei during meiotic prophase. In both fission yeasts the SPBs and nucleoli are at the leading ends of the moving nuclei. Astral and cytoplasmic microtubules were also prominent during meiotic divisions and sporulation. We further show that in S. versatilis the linear elements formed during meiotic prophase are similar to those in S. pombe. Tripartite synaptonemal complexes were never detected. Taken together, these findings suggest that S. pombe and S. versatilis share basic characteristics in the organization of microtubules and the structure and behaviour of nuclei during their meiotic cell cycle. The prominent differentiations of microtubules and nuclei may be involved in the pairing, recombination, and segregation of meiotic chromosomes.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Svoboda A, Bähler J, Kohli J","authors_abbrev":"Svoboda A et al.","canto_added_date":"2021-05-10 08:46:37","canto_curator_role":"JaponicusDB","canto_triage_status":"Cell composition or WT feature","citation":"Chromosoma 1995 Nov;104(3):203-14","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"1995","pubmed_entrez_date":"1995-11-01","pubmed_publication_date":"Nov 1995","terms_by_termid":{},"title":"Microtubule-driven nuclear movements and linear elements as meiosis-specific characteristics of the fission yeasts Schizosaccharomyces versatilis and Schizosaccharomyces pombe.","uniquename":"PMID:8529460"}


id: GO_REF:0000004
data:
  {"abstract":"Transitive assignments using UniProtKB keywords. The UniProtKB keyword controlled vocabulary has been created and used by the UniProt Knowledgebase (UniProtKB) to supply 10 different categories of information to UniProtKB entries. Further information on the UniProtKB keyword resource can be found at http://www.uniprot.org/docs/keywlist. <br>Further information on the UniProt annotation methods is available at  https://www.uniprot.org/help/manual_curation and https://www.uniprot.org/help/automatic_annotation.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"GOA curators","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Comments","uniquename":"GO_REF:0000004"}


id: PMID:1093
data:
  {"abstract":"(11 Cell extracts and extracellular culture fluids of species of the yeast genus Schizosaccharomyces exhibited exo-beta-(1 leads to 3)- and exo-beta-(1 leads to 6)-glucanase (EC 3.2.1.-) activities. (2) Using a combination of Sephadex G-100 and DEAE-cellulose chromatography, the exo-beta-(1 leads to 3)-glucanases from the cell extracts and culture fluid of Schizosaccharomyces japonicus var. versatilis were purified extensively. The enzymes from either location exhibited similar purification and other properties. (3) The purified enzymes hydrolysed the beta-(1 leads to 6)-glucosidic linkage in addition to the beta-(1 leads to 3) linkage. Heat denaturation, inhibition and electrophoretic studies indicated that both hydrolytic activities were properties of a single protein. Laminarin and pustulan hydrolysis followed Michaelis-Menten kinetics. The Km and V for laminarin hydrolysis were 6.25 mg/ml and 350 mumol of glucose released/min/mg protein, and for pustulan they were 166 mg/ml and 52 mumol of glucose released/min/mg protein. (4) The exo-beta-glucanase was assigned a molecular weight of 43 000. (5) the purified enzyme failed to hydrolyse isolated cell walls from either baker's yeast or Schizosaccharomyces pombe or to induce protoplast formation from intact cells of S. japonicus var. versatilis or Saccharomyces cerevisiae.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Fleet GH, Phaff HJ","authors_abbrev":"Fleet GH et al.","canto_added_date":"2021-05-10 08:46:37","canto_curator_role":"JaponicusDB","canto_triage_status":"Cell composition or WT feature","citation":"Biochim Biophys Acta 1975 Dec 18;410(2):318-32","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"1975","pubmed_entrez_date":"1975-12-18","pubmed_publication_date":"18 Dec 1975","terms_by_termid":{},"title":"Glucanases in Schizosaccharomyces. Isolation and properties of an exo-beta-glucanase from the cell extracts and culture fluid of Schizosaccharomyces japonicus var. versatilis.","uniquename":"PMID:1093"}


id: GO_REF:0000068
data:
  {"abstract":"We have created a standard template for classes each describing the metabolism, catabolism, or biosynthesis of a chemical entity (ChEBI) as a process. The underlying equivalence axiom templates are \"GO:0008152 and 'has participant' some X\" (metabolism), \"GO:0009056 and 'has input' some X\" (catabolism), \"GO:0009058 and 'has output' some X\" and (biosynthesis),  where X is a chemical entity (CHEBI:24431). The approach to combine GO and ChEBI has been described in the following publication: PMID:23895341.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"GO ontology editors","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Representation of metabolic triad (metabolism, catabolism, biosynthesis) as biological process in the Gene Ontology","uniquename":"GO_REF:0000068"}


id: PMID:31380845
data:
  {"abstract":"Lipid metabolism and its regulation are of interest to both basic and applied life sciences and biotechnology. In this regard, various yeast species are used as models in lipid metabolic research or for industrial lipid production. Lipid droplets are highly dynamic storage bodies and their cellular content represents a convenient readout of the lipid metabolic state. Fluorescence microscopy is a method of choice for quantitative analysis of cellular lipid droplets, as it relies on widely available equipment and allows analysis of individual lipid droplets. Furthermore, microscopic image analysis can be automated, greatly increasing overall analysis throughput. Here, we describe an experimental and analytical workflow for automated detection and quantitative description of individual lipid droplets in three different model yeast species: the fission yeasts Schizosaccharomyces pombe and Schizosaccharomyces japonicus, and the budding yeast Saccharomyces cerevisiae. Lipid droplets are visualized with BODIPY 493/503, and cell-impermeable fluorescent dextran is added to the culture media to help identify cell boundaries. Cells are subjected to 3D epifluorescence microscopy in green and blue channels and the resulting z-stack images are processed automatically by a MATLAB pipeline. The procedure outputs rich quantitative data on cellular lipid droplet content and individual lipid droplet characteristics in a tabular format suitable for downstream analyses in major spreadsheet or statistical packages. We provide example analyses of lipid droplet content under various conditions that affect cellular lipid metabolism.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Princová J, Schätz M, Ťupa O, Převorovský M","authors_abbrev":"Princová J et al.","canto_added_date":"2021-05-10 08:46:37","canto_curator_role":"JaponicusDB","canto_triage_status":"Method or reagent","citation":"J Vis Exp 2019 07 17;(149)","cv_annotations":{},"doi":"10.3791/59889","gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2019","pubmed_entrez_date":"2019-08-06","pubmed_publication_date":"17 07 2019","terms_by_termid":{},"title":"Analysis of Lipid Droplet Content in Fission and Budding Yeasts using Automated Image Processing.","uniquename":"PMID:31380845"}


id: PMID:8590481
data:
  {"abstract":"Fission yeasts form a small but heterogeneous group of ascomycetes and it is still unclear whether they should be subdivided into three genera (Schizosaccharomyces, Octosporomyces, Hasegawaea) or remain a single genus (Schizosaccharomyces). In order to decide whether a new genus Hasegawaea should be established for the species Schizosaccharomyces japonicus and Schizosaccharomyces versatilis, we have characterized the entire rDNA cluster in Schizosaccharomyces japonicus var. versatilis and compared it with the homologous region from Schizosaccharomyces pombe and with complete rRNA gene sequences from other yeast genera. From a phage genomic library a recombinant lambda phage containing the entire rDNA repeat unit was isolated. In this paper we report the primary sequence of the 18s, 5.8s and 25s rRNA coding regions. The S. japonicus var. versatilis rRNA genes are 1823 (18s), 158 (5.8s) and 3422 (25s) nucleotides long. The two sequences of the larger rRNA genes exhibit 95.7% (18s) and 93% (25s) similarity with the homologous genes from S. pombe. The differences between the rRNA genes of S. japonicus and S. pombe, however, are much smaller than the intrageneric differences within the rDNA sequences of other yeast genera. Therefore, subdivision of fission yeasts into the genera Schizosaccharomyces and Hasegawaea does not to seem to be justified.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Naehring J, Kiefer S, Wolf K","authors_abbrev":"Naehring J et al.","canto_added_date":"2021-05-10 08:46:37","canto_curator_role":"JaponicusDB","canto_triage_status":"Sequence feature or region","citation":"Curr Genet 1995 Sep;28(4):353-9","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"1995","pubmed_entrez_date":"1995-09-01","pubmed_publication_date":"Sep 1995","terms_by_termid":{},"title":"Nucleotide sequence of the Schizosaccharomyces japonicus var. versatilis ribosomal RNA gene cluster and its phylogenetic implications.","uniquename":"PMID:8590481"}


id: PMID:25866389
data:
  {"abstract":"Strategies to position the division apparatus exhibit a bewildering diversity [1], but how these mechanisms evolve remains virtually unknown. Here, we explore the plasticity of division site positioning in fission yeasts Schizosaccharomyces pombe and Schizosaccharomyces japonicus. We demonstrate that, whereas both species divide in the middle, only S. pombe uses the anillin Mid1 as a primary nucleus-derived cue to assemble the actomyosin ring at the equatorial cortex. We trace this variance to the divergence in subcellular targeting of Mid1 and show that duplication of an ancestral anillin early in the Schizosaccharomyces lineage may have led to subfunctionalization of the Mid1 orthologs. In contrast to S. pombe, medial assembly of the actomyosin ring in mitotic S. japonicus relies on the cortical anchor protein Cdc15 regulated by the tip-localized kinase Pom1. Our data suggest that division site placement is determined by cortical positioning of the actomyosin-plasma membrane linkers and that both identity of the linker and control of its subcellular targeting are highly modular.","alleles_by_uniquename":{"SJAG_00118:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_00118","name":"mid2delta","uniquename":"SJAG_00118:allele-1"},"SJAG_01143:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_01143","name":"mid1delta","uniquename":"SJAG_01143:allele-1"},"SJAG_01143:allele-2":{"allele_type":"partial_amino_acid_deletion","description":"711-723","gene_uniquename":"SJAG_01143","name":"mid1-delta(711-723 a.a.)","uniquename":"SJAG_01143:allele-2"},"SJAG_02392:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_02392","name":"pom1delta","uniquename":"SJAG_02392:allele-1"},"SJAG_03596:allele-1":{"allele_type":"amino_acid_mutation","description":"L264P,P442T","gene_uniquename":"SJAG_03596","name":"cdc15-A","uniquename":"SJAG_03596:allele-1"},"SJAG_04332:allele-1":{"allele_type":"amino_acid_mutation","description":"I30T,T572P","gene_uniquename":"SJAG_04332","name":"cdc25-D9","uniquename":"SJAG_04332:allele-1"}},"annotation_curators":[{"annotation_count":3,"community_curator":false,"file_name":null,"file_type":null,"name":"Val Wood","orcid":"0000-0001-6330-7526"},{"annotation_count":34,"community_curator":true,"file_name":null,"file_type":null,"name":"Ying Gu","orcid":null}],"annotation_details":{},"annotation_file_curators":[],"approved_date":"2021-05-12","authors":"Gu Y, Yam C, Oliferenko S","authors_abbrev":"Gu Y et al.","canto_added_date":"2021-05-10 02:37:54","canto_annotation_status":"APPROVED","canto_approved_date":"2021-05-16 08:31:49","canto_approver_orcid":"0000-0001-6330-7526","canto_curator_name":"Ying Gu","canto_curator_role":"community","canto_first_approved_date":"2021-05-12 14:24:14","canto_session_key":"bc986e831d627df1","canto_session_submitted_date":"2021-05-14 13:43:22","canto_triage_status":"Curatable","citation":"Curr Biol 2015 May 04;25(9):1187-94","cv_annotations":{"biological_process":[{"annotations":[115408],"is_not":false,"summary":null,"term":"GO:1902408"},{"annotations":[115409],"is_not":false,"summary":null,"term":"GO:1903617"},{"annotations":[115407],"is_not":true,"summary":null,"term":"GO:1902408"}],"cellular_component":[{"annotations":[115415,115416],"is_not":false,"summary":null,"term":"GO:0051286"},{"annotations":[115411,115413,115418,115420],"is_not":false,"summary":null,"term":"GO:1990753"},{"annotations":[115412,115414,115417,115419,115421],"is_not":false,"summary":null,"term":"GO:0110085"}],"multi_locus_phenotype":[{"annotations":[115399],"is_not":false,"summary":null,"term":"FYPO:0000231"},{"annotations":[115403],"is_not":false,"summary":null,"term":"FYPO:0005503"},{"annotations":[115405],"is_not":false,"summary":null,"term":"FYPO:0007177"},{"annotations":[115404],"is_not":false,"summary":null,"term":"FYPO:0000729"},{"annotations":[115401],"is_not":false,"summary":null,"term":"FYPO:0003946"},{"annotations":[115400],"is_not":false,"summary":null,"term":"FYPO:0000118"}],"qualitative_gene_expression":[{"annotations":[115391,115410],"is_not":false,"summary":null,"term":"PomGeneEx:0000011"}],"single_locus_phenotype":[{"annotations":[115392],"is_not":false,"summary":null,"term":"FYPO:0000231"},{"annotations":[115423,115424],"is_not":false,"summary":null,"term":"FYPO:0000731"},{"annotations":[115397],"is_not":false,"summary":null,"term":"FYPO:0005503"},{"annotations":[115394,115395,115402,115406],"is_not":false,"summary":null,"term":"FYPO:0007177"},{"annotations":[115425],"is_not":false,"summary":null,"term":"FYPO:0002026"},{"annotations":[115422],"is_not":false,"summary":null,"term":"FYPO:0003535"},{"annotations":[115393],"is_not":false,"summary":null,"term":"FYPO:0000729"},{"annotations":[115396],"is_not":false,"summary":null,"term":"FYPO:0007701"},{"annotations":[115389,115390],"is_not":false,"summary":null,"term":"FYPO:0000118"},{"annotations":[115398],"is_not":false,"summary":null,"term":"FYPO:0000644"}]},"doi":"10.1016/j.cub.2015.02.056","gene_count":8,"genes_by_uniquename":{"SJAG_00118":null,"SJAG_01143":null,"SJAG_01665":null,"SJAG_02392":null,"SJAG_03596":null,"SJAG_03703":null,"SJAG_04332":null,"SJAG_04498":null},"genotypes_by_uniquename":{"cdc15-A-L264P,P442T-amino_acid_mutation-expression-not_assayed_or_wild_type":{"display_name":"cdc15-A(L264P,P442T aa)","display_uniquename":"cdc15-A-L264P,P442T-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03596:allele-1","expression":"Not assayed or wild type"}]}]},"cdc15-A-L264P,P442T-amino_acid_mutation-expression-not_assayed_or_wild_type__mid1delta":{"display_name":"cdc15-A(L264P,P442T aa) mid1delta","display_uniquename":"cdc15-A-L264P,P442T-amino_acid_mutation-expression-not_assayed_or_wild_type__mid1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03596:allele-1","expression":"Not assayed or wild type"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_01143:allele-1","expression":"Null"}]}]},"cdc25-D9-I30T,T572P-amino_acid_mutation-expression-not_assayed_or_wild_type":{"display_name":"cdc25-D9(I30T,T572P aa)","display_uniquename":"cdc25-D9-I30T,T572P-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04332:allele-1","expression":"Not assayed or wild type"}]}]},"mid1-delta(711-723_a.a.)-711-723-partial_amino_acid_deletion-expression-not_assayed_or_wild_type":{"display_name":"mid1-delta(711-723 a.a.)(aa)","display_uniquename":"mid1-delta(711-723_a.a.)-711-723-partial_amino_acid_deletion-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01143:allele-2","expression":"Not assayed or wild type"}]}]},"mid1delta":{"display_name":"mid1delta","display_uniquename":"mid1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01143:allele-1","expression":"Null"}]}]},"mid1delta__mid2delta":{"display_name":"mid1delta mid2delta","display_uniquename":"mid1delta__mid2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01143:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_00118:allele-1","expression":"Null"}]}]},"mid1delta__pom1delta":{"display_name":"mid1delta pom1delta","display_uniquename":"mid1delta__pom1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01143:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_02392:allele-1","expression":"Null"}]}]},"mid2delta":{"display_name":"mid2delta","display_uniquename":"mid2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00118:allele-1","expression":"Null"}]}]},"pom1delta":{"display_name":"pom1delta","display_uniquename":"pom1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02392:allele-1","expression":"Null"}]}]}},"ltp_gene_count":8,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2015","pubmed_entrez_date":"2015-04-14","pubmed_publication_date":"04 May 2015","terms_by_termid":{"FYECO:0000004":null,"FYECO:0000005":null,"FYECO:0000137":null,"FYECO:0000162":null,"FYPO:0000118":null,"FYPO:0000231":null,"FYPO:0000644":null,"FYPO:0000729":null,"FYPO:0000731":null,"FYPO:0002026":null,"FYPO:0003535":null,"FYPO:0003946":null,"FYPO:0005503":null,"FYPO:0007177":null,"FYPO:0007701":null,"FYPO_EXT:0000001":null,"FYPO_EXT:0000002":null,"GO:0000085":null,"GO:0000087":null,"GO:0051286":null,"GO:0051325":null,"GO:0051329":null,"GO:0110085":null,"GO:1902408":null,"GO:1903617":null,"GO:1990753":null,"PomGeneEx:0000011":null},"title":"Rewiring of cellular division site selection in evolution of fission yeasts.","uniquename":"PMID:25866389"}


id: GO_REF:0000036
data:
  {"abstract":"The Gene Ontology Consortium uses the IC (Inferred by Curator) evidence code when an annotation cannot be supported by any direct evidence, but can be inferred by GO annotations that have been annotated to the same gene/gene product identifier in conjunction with the curator's knowledge of biology (supporting GO annotations must not be IC-evidenced). In many cases an IC-evidenced annotation simply applies the same reference that was used in the supporting GO annotation.  The use of IC evidence code in an annotation with reference GO_REF:0000036 signifies a curator inferred the GO term based on evidence from multiple sources of evidence/GO annotations. The 'with/from' field in these annotations will therefore supply more than one GO identifier, obtained from the set of supporting GO annotations assigned to the same gene/gene product identifier which cite publicly-available references.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"GO Annotation working group","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Manual annotations that require more than one source of functional data to support the assignment of the associated GO term","uniquename":"GO_REF:0000036"}


id: PMID:28655757
data:
  {"abstract":"Cytokinesis in many eukaryotes involves a tension-generating actomyosin-based contractile ring. Many components of actomyosin rings turn over during contraction, although the significance of this turnover has remained enigmatic. Here, using  Schizosaccharomyces japonicus , we investigate the role of turnover of actin and myosin II in its contraction. Actomyosin ring components self-organize into ∼1-µm-spaced clusters instead of undergoing full-ring contraction in the absence of continuous actin polymerization. This effect is reversed when actin filaments are stabilized. We tested the idea that the function of turnover is to ensure actin filament homeostasis in a synthetic system, in which we abolished turnover by fixing rings in cell ghosts with formaldehyde. We found that these rings contracted fully upon exogenous addition of a vertebrate myosin. We conclude that actin turnover is required to maintain actin filament homeostasis during ring contraction and that the requirement for turnover can be bypassed if homeostasis is achieved artificially.","alleles_by_uniquename":{},"annotation_curators":[{"annotation_count":5,"community_curator":false,"file_name":null,"file_type":null,"name":"Snezhana Oliferenko","orcid":"0000-0002-8138-6851"}],"annotation_details":{},"annotation_file_curators":[],"approved_date":"2021-07-09","authors":"Chew TG, Huang J, Palani S, Sommese R, Kamnev A, Hatano T, Gu Y, Oliferenko S, Sivaramakrishnan S, Balasubramanian MK","authors_abbrev":"Chew TG et al.","canto_added_date":"2021-05-10 08:46:37","canto_annotation_status":"APPROVED","canto_approved_date":"2021-07-09 13:45:34","canto_approver_orcid":"0000-0002-8138-6851","canto_curator_name":"Snezhana Oliferenko","canto_curator_role":"JaponicusDB","canto_first_approved_date":"2021-07-09 13:45:34","canto_session_key":"5ead7a365a97d8ab","canto_session_submitted_date":"2021-07-09 13:45:28","canto_triage_status":"Curatable","citation":"J Cell Biol 2017 09 04;216(9):2657-2667","cv_annotations":{"cellular_component":[{"annotations":[115438,115439,115440,115441,115442],"is_not":false,"summary":null,"term":"GO:0005826"}]},"doi":"10.1083/jcb.201701104","gene_count":5,"genes_by_uniquename":{"SJAG_01665":null,"SJAG_02431":null,"SJAG_03596":null,"SJAG_03703":null,"SJAG_04498":null},"genotypes_by_uniquename":{},"ltp_gene_count":5,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2017","pubmed_entrez_date":"2017-06-29","pubmed_publication_date":"04 09 2017","terms_by_termid":{"GO:0005826":null},"title":"Actin turnover maintains actin filament homeostasis during cytokinetic ring contraction.","uniquename":"PMID:28655757"}


id: GO_REF:0000105
data:
  {"abstract":"Gene Ontology annotation based on predicted cytoplasmic tRNAs using tRNAscan-SE analysis (doi: 10.1093/nar/25.5.0955) of the Drosophila melanogaster genome (2002). Annotations have been reviewed by FlyBase (2015) and found to be consistent when compared with the most recent tRNAscan-SE analysis of the genome (http://gtrnadb.ucsc.edu/genomes/eukaryota/Dmela6/).","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"FlyBase","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Gene Ontology annotation of transfer RNAs based on tRNAscan-SE analysis of the Drosophila melanogaster genome (2002).","uniquename":"GO_REF:0000105"}


id: GO_REF:0000061
data:
  {"abstract":"We have created a standard template for classes describing molecular function involved in other biological processes. The underlying equivalence axiom template is \"P and 'part_of' some W\", where P is a molecular function and W is a biological processes.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"GO ontology editors","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Representation of a molecular function involved in a biological process in the Gene Ontology","uniquename":"GO_REF:0000061"}


id: GO_REF:0000118
data:
  {"abstract":"TreeGrafter is a software tool for annotating protein sequences using pre-annotated PANTHER phylogenetic trees. TreeGrafter takes an input query protein sequence, finds the best matching homologous family, and then grafts it to the best location in the tree. It then annotates the query sequence by propagating annotations from the appropriate ancestral node(s) in the reference tree, which were manually annotated using the PAN-GO method (see GOREF_0000033). This method is integrated into InterProScan, which produces annotations to millions of genes across tens of thousands of organisms.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Haiming Tang, Dustin Ebert, Matthias Blum, Robert Finn, Paul Thomas ","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"TreeGrafter-generated GO annotations","uniquename":"GO_REF:0000118"}


id: PMID:33947067
data:
  {"abstract":"The fission yeast  Schizosaccharomyces japonicus , comprising  S. japonicus  var.  japonicus  and  S. japonicus  var.  versatilis  varieties, has unique characteristics such as striking hyphal growth not seen in other  Schizosaccharomyces  species; however, information on its diversity and evolution, in particular mating and sporulation, remains limited. Here we compared the growth and mating phenotypes of 17 wild strains of  S. japonicus , including eight  S. japonicus  var.  japonicus  strains newly isolated from an insect ( Drosophila ). Unlike existing wild strains isolated from fruits/plants, the strains isolated from  Drosophila  sporulated at high frequency even under nitrogen-abundant conditions. In addition, one of the strains from  Drosophila  was stained by iodine vapor, although the type strain of  S. japonicus  var.  japonicus  is not stained. Sequence analysis further showed that the nucleotide and amino acid sequences of pheromone-related genes have diversified among the eight strains from  Drosophila , suggesting crossing between  S. japonicus  cells of different genetic backgrounds occurs frequently in this insect. Much of yeast ecology remains unclear, but our findings suggest that insects such as  Drosophila  might be a good niche for mating and sporulation, and will provide a basis for the understanding of sporulation mechanisms via signal transduction, as well as the ecology and evolution of yeast.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Seike T, Sakata N, Matsuda F, Furusawa C","authors_abbrev":"Seike T et al.","canto_added_date":"2021-05-10 08:46:37","canto_curator_role":"JaponicusDB","canto_triage_status":"Cell composition or WT feature","citation":"J Fungi (Basel) 2021 Apr 29;7(5)","cv_annotations":{},"doi":"10.3390/jof7050350","gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2021","pubmed_entrez_date":"2021-05-05","pubmed_publication_date":"29 Apr 2021","terms_by_termid":{},"title":"Elevated Sporulation Efficiency in Fission Yeast  Schizosaccharomyces japonicus  Strains Isolated from  Drosophila .","uniquename":"PMID:33947067"}


id: PMID:32109380
data:
  {"abstract":"Eukaryotic genomes are organized within the nucleus through interactions with inner nuclear membrane (INM) proteins. How chromatin tethering to the INM is controlled in interphase and how this process contributes to subsequent mitotic nuclear envelope (NE) remodeling remains unclear. We have probed these fundamental questions using the fission yeast Schizosaccharomyces japonicus, which breaks and reforms the NE during mitosis. We show that attachments between heterochromatin and the transmembrane Lem2-Nur1 complex at the INM are remodeled in interphase by the ESCRT-III/Vps4 machinery. Failure of ESCRT-III/Vps4 to release Lem2-Nur1 from heterochromatin leads to persistent association of chromosomes with the INM throughout mitosis. At mitotic exit, such trapping of Lem2-Nur1 on heterochromatin prevents it from re-establishing nucleocytoplasmic compartmentalization. Our work identifies the Lem2-Nur1 complex as a substrate for the nuclear ESCRT machinery and explains how the dynamic tethering of chromosomes to the INM is linked to the establishment of nuclear compartmentalization.","alleles_by_uniquename":{"SJAG_01050:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_01050","name":"nur1delta","uniquename":"SJAG_01050:allele-1"},"SJAG_01242:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_01242","name":"vps32delta","uniquename":"SJAG_01242:allele-1"},"SJAG_01330:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_01330","name":"mad2delta","uniquename":"SJAG_01330:allele-1"},"SJAG_01745:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_01745","name":"lem2delta","uniquename":"SJAG_01745:allele-1"},"SJAG_03250:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_03250","name":"sst4delta","uniquename":"SJAG_03250:allele-1"},"SJAG_03783:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_03783","name":"vps24delta","uniquename":"SJAG_03783:allele-1"},"SJAG_03892:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_03892","name":"vps4delta","uniquename":"SJAG_03892:allele-1"},"SJAG_03892:allele-2":{"allele_type":"amino_acid_mutation","description":"E233Q","gene_uniquename":"SJAG_03892","name":"Vps4-EQ","uniquename":"SJAG_03892:allele-2"},"SJAG_03892:allele-3":{"allele_type":"amino_acid_mutation","description":"I307T L327S","gene_uniquename":"SJAG_03892","name":"vps4-ts","uniquename":"SJAG_03892:allele-3"},"SJAG_04349:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_04349","name":"vps28delta","uniquename":"SJAG_04349:allele-1"},"SJAG_04489:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_04489","name":"did4delta","uniquename":"SJAG_04489:allele-1"},"SJAG_04912:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_04912","name":"vps25delta","uniquename":"SJAG_04912:allele-1"},"SJAG_05207:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_05207","name":"cmp7delta","uniquename":"SJAG_05207:allele-1"}},"annotation_curators":[{"annotation_count":53,"community_curator":false,"file_name":null,"file_type":null,"name":"Snezhana Oliferenko","orcid":"0000-0002-8138-6851"}],"annotation_details":{},"annotation_file_curators":[],"approved_date":"2021-06-17","authors":"Pieper GH, Sprenger S, Teis D, Oliferenko S","authors_abbrev":"Pieper GH et al.","canto_added_date":"2021-05-10 02:37:07","canto_annotation_status":"APPROVED","canto_approved_date":"2021-06-17 17:46:25","canto_approver_orcid":"0000-0002-8138-6851","canto_curator_name":"Snezhana Oliferenko","canto_curator_role":"JaponicusDB","canto_first_approved_date":"2021-06-17 17:46:25","canto_session_key":"89c611dbc0229af1","canto_session_submitted_date":"2021-06-17 17:46:16","canto_triage_status":"Curatable","citation":"Dev Cell 2020 04 06;53(1):27-41.e6","cv_annotations":{"cellular_component":[{"annotations":[115498],"is_not":false,"summary":null,"term":"GO:0000781"},{"annotations":[115496],"is_not":false,"summary":null,"term":"GO:0005783"},{"annotations":[115518],"is_not":false,"summary":null,"term":"GO:0000792"},{"annotations":[115523],"is_not":false,"summary":null,"term":"GO:1990343"},{"annotations":[115480],"is_not":false,"summary":null,"term":"GO:0000776"},{"annotations":[115500],"is_not":false,"summary":null,"term":"GO:0005771"},{"annotations":[115502],"is_not":false,"summary":null,"term":"GO:0032585"},{"annotations":[115501,115503],"is_not":false,"summary":null,"term":"GO:0031965"},{"annotations":[115524],"is_not":false,"summary":null,"term":"GO:0005643"},{"annotations":[115481,115486,115504],"is_not":false,"summary":null,"term":"GO:0005816"}],"molecular_function":[{"annotations":[115479],"is_not":false,"summary":null,"term":"GO:0043515"}],"multi_locus_phenotype":[{"annotations":[115509],"is_not":false,"summary":null,"term":"FYPO:0007771"},{"annotations":[115520],"is_not":false,"summary":null,"term":"FYPO:0001132"}],"single_locus_phenotype":[{"annotations":[115505,115506,115507],"is_not":false,"summary":null,"term":"FYPO:0001440"},{"annotations":[115516,115517],"is_not":false,"summary":null,"term":"FYPO:0001370"},{"annotations":[115519],"is_not":false,"summary":null,"term":"FYPO:0004718"},{"annotations":[115484,115485,115491,115492,115494,115495,115508,115510,115511,115512,115513,115514,115515],"is_not":false,"summary":null,"term":"FYPO:0007771"},{"annotations":[115497],"is_not":false,"summary":null,"term":"FYPO:0004318"},{"annotations":[115488],"is_not":false,"summary":null,"term":"FYPO:0007073"},{"annotations":[115482,115483,115489,115490],"is_not":false,"summary":null,"term":"FYPO:0007772"},{"annotations":[115493],"is_not":false,"summary":null,"term":"FYPO:0007770"},{"annotations":[115499],"is_not":false,"summary":null,"term":"FYPO:0003780"},{"annotations":[115487],"is_not":false,"summary":null,"term":"FYPO:0003657"},{"annotations":[115521,115522],"is_not":false,"summary":null,"term":"FYPO:0001234"}]},"doi":"10.1016/j.devcel.2020.01.028","gene_count":15,"genes_by_uniquename":{"SJAG_00684":null,"SJAG_01050":null,"SJAG_01242":null,"SJAG_01330":null,"SJAG_01601":null,"SJAG_01745":null,"SJAG_01777":null,"SJAG_03250":null,"SJAG_03299":null,"SJAG_03783":null,"SJAG_03892":null,"SJAG_04349":null,"SJAG_04489":null,"SJAG_04912":null,"SJAG_05207":null},"genotypes_by_uniquename":{"Vps4-EQ-E233Q-amino_acid_mutation-expression-not_assayed_or_wild_type":{"display_name":"Vps4-EQ(E233Q aa)","display_uniquename":"Vps4-EQ-E233Q-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03892:allele-2","expression":"Not assayed or wild type"}]}]},"cmp7delta":{"display_name":"cmp7delta","display_uniquename":"cmp7delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_05207:allele-1","expression":"Null"}]}]},"cmp7delta__vps4-ts-I307T_L327S-amino_acid_mutation-expression-not_assayed_or_wild_type":{"display_name":"cmp7delta vps4-ts(I307T L327S aa)","display_uniquename":"cmp7delta__vps4-ts-I307T_L327S-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_05207:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_03892:allele-3","expression":"Not assayed or wild type"}]}]},"did4delta":{"display_name":"did4delta","display_uniquename":"did4delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04489:allele-1","expression":"Null"}]}]},"lem2delta":{"display_name":"lem2delta","display_uniquename":"lem2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01745:allele-1","expression":"Null"}]}]},"mad2delta":{"display_name":"mad2delta","display_uniquename":"mad2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01330:allele-1","expression":"Null"}]}]},"nur1delta":{"display_name":"nur1delta","display_uniquename":"nur1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01050:allele-1","expression":"Null"}]}]},"nur1delta__vps4delta":{"display_name":"nur1delta vps4delta","display_uniquename":"nur1delta__vps4delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01050:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_03892:allele-1","expression":"Null"}]}]},"sst4delta":{"display_name":"sst4delta","display_uniquename":"sst4delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03250:allele-1","expression":"Null"}]}]},"vps24delta":{"display_name":"vps24delta","display_uniquename":"vps24delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03783:allele-1","expression":"Null"}]}]},"vps25delta":{"display_name":"vps25delta","display_uniquename":"vps25delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04912:allele-1","expression":"Null"}]}]},"vps28delta":{"display_name":"vps28delta","display_uniquename":"vps28delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04349:allele-1","expression":"Null"}]}]},"vps32delta":{"display_name":"vps32delta","display_uniquename":"vps32delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01242:allele-1","expression":"Null"}]}]},"vps4-ts-I307T_L327S-amino_acid_mutation-expression-not_assayed_or_wild_type":{"display_name":"vps4-ts(I307T L327S aa)","display_uniquename":"vps4-ts-I307T_L327S-amino_acid_mutation-expression-not_assayed_or_wild_type","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03892:allele-3","expression":"Not assayed or wild type"}]}]},"vps4delta":{"display_name":"vps4delta","display_uniquename":"vps4delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03892:allele-1","expression":"Null"}]}]}},"ltp_gene_count":15,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[{"evidence":"Reconstituted Complex","gene_uniquename":"SJAG_01242","interactor_uniquename":"SJAG_03892","reference_uniquename":"PMID:32109380","throughput":"low"},{"evidence":"Reconstituted Complex","gene_uniquename":"SJAG_04489","interactor_uniquename":"SJAG_03892","reference_uniquename":"PMID:32109380","throughput":"low"},{"evidence":"Reconstituted Complex","gene_uniquename":"SJAG_05207","interactor_uniquename":"SJAG_01242","reference_uniquename":"PMID:32109380","throughput":"low"},{"evidence":"Reconstituted Complex","gene_uniquename":"SJAG_05207","interactor_uniquename":"SJAG_01745","reference_uniquename":"PMID:32109380","throughput":"low"},{"evidence":"Reconstituted Complex","gene_uniquename":"SJAG_05207","interactor_uniquename":"SJAG_03892","reference_uniquename":"PMID:32109380","throughput":"low"}],"publication_year":"2020","pubmed_entrez_date":"2020-02-29","pubmed_publication_date":"06 04 2020","terms_by_termid":{"FYECO:0000004":null,"FYECO:0000137":null,"FYPO:0001132":null,"FYPO:0001234":null,"FYPO:0001370":null,"FYPO:0001440":null,"FYPO:0003657":null,"FYPO:0003780":null,"FYPO:0004318":null,"FYPO:0004718":null,"FYPO:0007073":null,"FYPO:0007770":null,"FYPO:0007771":null,"FYPO:0007772":null,"FYPO_EXT:0000001":null,"FYPO_EXT:0000002":null,"GO:0000093":null,"GO:0000776":null,"GO:0000781":null,"GO:0000792":null,"GO:0000815":null,"GO:0005643":null,"GO:0005771":null,"GO:0005783":null,"GO:0005816":null,"GO:0031965":null,"GO:0032585":null,"GO:0043515":null,"GO:1990343":null},"title":"ESCRT-III/Vps4 Controls Heterochromatin-Nuclear Envelope Attachments.","uniquename":"PMID:32109380"}


id: PMID:26263485
data:
  {"abstract":"Cytokinesis must be regulated in time and space in order to preserve genome integrity during cell proliferation and to allow daughter cells to adopt distinct fates and geometries during differentiation. The fission yeast Schizosaccharomyces pombe has been a popular model organism for understanding spatiotemporal regulation of cytokinesis in a symmetrically dividing cell. Recent work on another member of the same genus, Schisozaccharomyces japonicus, suggests that S. pombe may have evolved an unusual division site placement mechanism based on a recently duplicated anillin paralog. Here we discuss an extraordinary evolutionary plasticity of cytokinesis within the fission yeast clade and argue that the comparative cell biology approach may provide functional insights beyond those afforded by scrutinizing individual model species.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Gu Y, Oliferenko S","authors_abbrev":"Gu Y et al.","canto_added_date":"2023-05-04 00:40:04","canto_curator_role":"JaponicusDB","canto_triage_status":"Review or comment","citation":"Curr Opin Microbiol 2015 Dec;28:18-25","cv_annotations":{},"doi":"10.1016/j.mib.2015.07.011","gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2015","pubmed_entrez_date":"2015-08-12","pubmed_publication_date":"Dec 2015","terms_by_termid":{},"title":"Comparative biology of cell division in the fission yeast clade.","uniquename":"PMID:26263485"}


id: PMID:10379403
data:
  {"abstract":"The order Schizosaccharomycetales contains a dimorphic and two yeast species. Sch. japonicus can form both yeast cells and mycelium, depending on the substrate and the culturing conditions. Sch. pombe is a strictly unicellular organism, but it can be forced to form mycelial cell chains by inactivating members of the sep gene family. The mutations in most of the sep genes confer pleitropic phenotypes indicating functional involvement in MAP-kinase-mediated signalling pathways. Two of them were found to encode transcription factor homologues of other eukaryotes.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Sipiczki M, Grallert A, Miklós I, Zilahi E, Bozsik A, Szilágyi Z","authors_abbrev":"Sipiczki M et al.","canto_added_date":"2023-05-04 00:40:04","canto_annotation_status":"SESSION_CREATED","canto_curator_role":"JaponicusDB","canto_session_key":"eb5db4e2ead0caf4","canto_triage_status":"Cell composition or WT feature","citation":"Acta Microbiol Immunol Hung 1999;46(2-3):297-302","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"1999","pubmed_entrez_date":"1999-06-24","pubmed_publication_date":"1999","terms_by_termid":{},"title":"Genetics, physiology and cytology of yeast-mycelial dimorphism in fission yeasts.","uniquename":"PMID:10379403"}


id: PMID:23090706
data:
  {"abstract":"DNA damage response includes DNA repair, nucleotide metabolism and even a control of cell fates including differentiation, cell death pathway or some combination of these. The responses to DNA damage differ from species to species. Here we aim to delineate the checkpoint pathway in the dimorphic fission yeast Schizosaccharomyces japonicus, where DNA damage can trigger a differentiation pathway that is a switch from a bidirectional yeast growth mode to an apical hyphal growth mode, and the switching is regulated via a checkpoint kinase, Chk1. This Chk1-dependent switch to hyphal growth is activated with even low doses of agents that damage DNA; therefore, we reasoned that this switch may depend on other genes orthologous to the components of the classical Sz. pombe Chk1-dependent DNA checkpoint pathway. As an initial test of this hypothesis, we assessed the effects of mutations in Sz. japonicus orthologs of Sz. pombe checkpoint genes on this switch from bidirectional to hyphal growth. The same set of DNA checkpoint genes was confirmed in Sz. japonicus. We tested the effect of each DNA checkpoint mutants on hyphal differentiation by DNA damage. We found that the Sz. japonicus hyphal differentiation pathway was dependent on Sz. japonicus orthologs of Sz. pombe checkpoint genes-(SP)rad3, (SP)rad26, (SP)rad9, (SP)rad1, (SP)rad24, (SP)rad25, (SP)crb2, and (SP)chk1-that function in the DNA damage checkpoint pathway, but was not dependent on orthologs of two Sz. pombe genes-(SP)cds1 or (SP)mrc1-that function in the DNA replication checkpoint pathway. These findings indicated that although the role of each component of the DNA damage checkpoint and DNA replication checkpoint is mostly same between the two fission yeasts, the DNA damage checkpoint was the only pathway that governed DNA damage-dependent hyphal growth. We also examined whether DNA damage checkpoint signaling engaged in functional crosstalk with other hyphal differentiation pathways because hyphal differentiation can also be triggered by nutritional stress. Here, we discovered genetic interactions that indicated that the cAMP pathway engaged in crosstalk with Chk1-dependent signaling.","alleles_by_uniquename":{"SJAG_00364:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_00364","name":"rad3delta","uniquename":"SJAG_00364:allele-1"},"SJAG_00429:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_00429","name":"rad26delta","uniquename":"SJAG_00429:allele-1"},"SJAG_01205:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_01205","name":"crb2delta","uniquename":"SJAG_01205:allele-1"},"SJAG_01605:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_01605","name":"rad9delta","uniquename":"SJAG_01605:allele-1"},"SJAG_01680:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_01680","name":"chk1delta","uniquename":"SJAG_01680:allele-1"},"SJAG_01680:allele-2":{"allele_type":"amino_acid_mutation","description":"P470L","gene_uniquename":"SJAG_01680","name":"chk1-P470L","synonyms":[{"name":"chk1- hyperactive (chk1-hyp)","reference":"PMID:23090706","type":"exact"},{"name":"chk1-hyperactive (chk1-hyp)","reference":"PMID:20368354","type":"exact"}],"uniquename":"SJAG_01680:allele-2"},"SJAG_02266:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_02266","name":"rad24delta","uniquename":"SJAG_02266:allele-1"},"SJAG_02576:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_02576","name":"rad25delta","uniquename":"SJAG_02576:allele-1"},"SJAG_02771:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_02771","name":"rad1delta","uniquename":"SJAG_02771:allele-1"},"SJAG_03720:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_03720","name":"tel1delta","uniquename":"SJAG_03720:allele-1"},"SJAG_04287:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_04287","name":"cds1delta","uniquename":"SJAG_04287:allele-1"},"SJAG_04671:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_04671","name":"mrc1delta","uniquename":"SJAG_04671:allele-1"}},"annotation_curators":[{"annotation_count":28,"community_curator":true,"file_name":null,"file_type":null,"name":"Sho Okamoto","orcid":null}],"annotation_details":{},"annotation_file_curators":[],"approved_date":"2022-11-21","authors":"Furuya K, Niki H","authors_abbrev":"Furuya K et al.","canto_added_date":"2021-05-10 08:46:37","canto_annotation_status":"APPROVED","canto_approved_date":"2022-11-21 17:21:20","canto_approver_orcid":"0000-0002-8138-6851","canto_curator_name":"Sho Okamoto","canto_curator_role":"community","canto_first_approved_date":"2022-11-21 17:21:20","canto_session_key":"5f68f94ee65a99b7","canto_session_submitted_date":"2022-11-21 17:21:13","canto_triage_status":"Curatable","citation":"Curr Genet 2012 Dec;58(5-6):291-303","cv_annotations":{"multi_locus_phenotype":[{"annotations":[115614],"is_not":false,"summary":null,"term":"FYPO:0007790"},{"annotations":[115620],"is_not":false,"summary":null,"term":"FYPO:0000085"},{"annotations":[115617],"is_not":false,"summary":null,"term":"FYPO:0000088"}],"single_locus_phenotype":[{"annotations":[115605,115606,115607,115608,115609,115610,115612,115613],"is_not":false,"summary":null,"term":"FYPO:0007782"},{"annotations":[115631],"is_not":false,"summary":null,"term":"FYPO:0007774"},{"annotations":[115630],"is_not":false,"summary":null,"term":"FYPO:0001690"},{"annotations":[115604,115611],"is_not":false,"summary":null,"term":"FYPO:0007790"},{"annotations":[115629],"is_not":false,"summary":null,"term":"FYPO:0004693"},{"annotations":[115618,115619,115624,115625,115626],"is_not":false,"summary":null,"term":"FYPO:0000085"},{"annotations":[115615,115616,115621,115622,115623],"is_not":false,"summary":null,"term":"FYPO:0000088"},{"annotations":[115627,115628],"is_not":false,"summary":null,"term":"FYPO:0001234"}]},"doi":"10.1007/s00294-012-0384-4","gene_count":11,"genes_by_uniquename":{"SJAG_00364":null,"SJAG_00429":null,"SJAG_01205":null,"SJAG_01605":null,"SJAG_01680":null,"SJAG_02266":null,"SJAG_02576":null,"SJAG_02771":null,"SJAG_03720":null,"SJAG_04287":null,"SJAG_04671":null},"genotypes_by_uniquename":{"cds1delta":{"display_name":"cds1delta","display_uniquename":"cds1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04287:allele-1","expression":"Null"}]}]},"chk1-P470L-P470L-amino_acid_mutation-expression-not_assayed_or_wild_type__rad24delta":{"display_name":"chk1-P470L(aa) rad24delta","display_uniquename":"chk1-P470L-P470L-amino_acid_mutation-expression-not_assayed_or_wild_type__rad24delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01680:allele-2","expression":"Not assayed or wild type"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_02266:allele-1","expression":"Null"}]}]},"chk1delta":{"display_name":"chk1delta","display_uniquename":"chk1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01680:allele-1","expression":"Null"}]}]},"chk1delta__crb2delta":{"display_name":"chk1delta crb2delta","display_uniquename":"chk1delta__crb2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01680:allele-1","expression":"Null"}]},{"expressed_alleles":[{"allele_uniquename":"SJAG_01205:allele-1","expression":"Null"}]}]},"crb2delta":{"display_name":"crb2delta","display_uniquename":"crb2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01205:allele-1","expression":"Null"}]}]},"mrc1delta":{"display_name":"mrc1delta","display_uniquename":"mrc1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04671:allele-1","expression":"Null"}]}]},"rad1delta":{"display_name":"rad1delta","display_uniquename":"rad1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02771:allele-1","expression":"Null"}]}]},"rad24delta":{"display_name":"rad24delta","display_uniquename":"rad24delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02266:allele-1","expression":"Null"}]}]},"rad25delta":{"display_name":"rad25delta","display_uniquename":"rad25delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02576:allele-1","expression":"Null"}]}]},"rad26delta":{"display_name":"rad26delta","display_uniquename":"rad26delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00429:allele-1","expression":"Null"}]}]},"rad3delta":{"display_name":"rad3delta","display_uniquename":"rad3delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00364:allele-1","expression":"Null"}]}]},"rad9delta":{"display_name":"rad9delta","display_uniquename":"rad9delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01605:allele-1","expression":"Null"}]}]},"tel1delta":{"display_name":"tel1delta","display_uniquename":"tel1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03720:allele-1","expression":"Null"}]}]}},"ltp_gene_count":11,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2012","pubmed_entrez_date":"2012-10-24","pubmed_publication_date":"Dec 2012","terms_by_termid":{"FYECO:0000005":null,"FYECO:0000137":null,"FYECO:0000314":null,"FYPO:0000085":null,"FYPO:0000088":null,"FYPO:0001234":null,"FYPO:0001690":null,"FYPO:0004693":null,"FYPO:0007774":null,"FYPO:0007782":null,"FYPO:0007790":null,"FYPO_EXT:0000001":null,"FYPO_EXT:0000002":null,"FYPO_EXT:0000003":null},"title":"Hyphal differentiation induced via a DNA damage checkpoint-dependent pathway engaged in crosstalk with nutrient stress signaling in Schizosaccharomyces japonicus.","uniquename":"PMID:23090706"}


id: PMID:33791858
data:
  {"abstract":"Mitogen-activated protein kinase (MAPK) signalling pathways regulate multiple cellular functions in eukaryotic organisms in response to environmental cues, including the dynamic remodeling of the actin cytoskeleton. The fission yeast S. pombe is an optimal model to investigate the conserved regulatory mechanisms of cytokinesis, which relies in an actomyosin-based contractile ring (CAR) that prompts the physical separation of daughter cells during cellular division. Our group has recently shown that p38 MAPK ortholog Sty1, the core component of the stress-activated pathway (SAPK), negatively modulates CAR assembly and integrity in S. pombe during actin cytoskeletal damage induced with Latrunculin A and in response to environmental stress. This response involves downregulation of protein levels of the formin For3, which assembles actin filaments for cables and the CAR, likely through an ubiquitin-mediated degradation mechanism. Contrariwise, Sty1 function positively reinforces CAR assembly during stress in the close relative dimorphic fission yeast S. japonicus. The opposite effect of SAPK signaling on CAR integrity may represent an evolutionary refined adaptation to cope with the marked differences in cytokinesis onset in both fission yeast species.","alleles_by_uniquename":{"SJAG_00266:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_00266","name":"atf1delta","uniquename":"SJAG_00266:allele-1"},"SJAG_02592:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_02592","name":"sty1delta","uniquename":"SJAG_02592:allele-1"}},"annotation_curators":[{"annotation_count":4,"community_curator":true,"file_name":null,"file_type":null,"name":"Elisa Gómez Gil","orcid":null}],"annotation_details":{},"annotation_file_curators":[],"approved_date":"2021-09-14","authors":"Madrid M, Gómez-Gil E, Cansado J","authors_abbrev":"Madrid M et al.","canto_added_date":"2021-05-10 08:46:37","canto_annotation_status":"APPROVED","canto_approved_date":"2021-09-14 21:24:28","canto_approver_orcid":"0000-0002-8138-6851","canto_curator_name":"Elisa Gómez Gil","canto_curator_role":"community","canto_first_approved_date":"2021-09-14 21:24:28","canto_session_key":"84ac20b0ff20a3cd","canto_session_submitted_date":"2021-09-14 16:45:32","canto_triage_status":"Curatable","citation":"Curr Genet 2021 Oct;67(5):715-721","cv_annotations":{"PSI-MOD":[{"annotations":[115278],"is_not":false,"summary":null,"term":"MOD:00696"}],"single_locus_phenotype":[{"annotations":[115277],"is_not":false,"summary":null,"term":"FYPO:0002998"},{"annotations":[115275,115276],"is_not":false,"summary":null,"term":"FYPO:0000107"}]},"doi":"10.1007/s00294-021-01155-6","gene_count":2,"genes_by_uniquename":{"SJAG_00266":null,"SJAG_02592":null},"genotypes_by_uniquename":{"atf1delta":{"display_name":"atf1delta","display_uniquename":"atf1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_00266:allele-1","expression":"Null"}]}]},"sty1delta":{"display_name":"sty1delta","display_uniquename":"sty1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02592:allele-1","expression":"Null"}]}]}},"ltp_gene_count":2,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2021","pubmed_entrez_date":"2021-04-01","pubmed_publication_date":"Oct 2021","terms_by_termid":{"FYECO:0000248":null,"FYPO:0000107":null,"FYPO:0002998":null,"FYPO_EXT:0000001":null,"GO:0120306":null,"MOD:00696":null},"title":"Negative control of cytokinesis by stress-activated MAPK signaling.","uniquename":"PMID:33791858"}


id: PMID:33837814
data:
  {"abstract":"The capability of RNA isolation with good efficiency and high quality is essential for a downstream application such as RNA sequencing. It requires successful cell culturing and an effective RNA isolation method. Although effective methods are available, production of the homogenous mycelia and extraction of good-quality mycelial RNA from true invasive hyphae, which penetrated into the agar plates, are difficult. To overcome these problems, the aim of this study was to develop technical modifications which allow production of homogenous mycelial biomass without extra stimuli agents and improve quality of the RNA extracted from the fungal hyphae. Our alternative culture medium was suitable for production both yeast-phase cells and hyphae of the Schizosaccharomyces japonicus and other dimorphic species, such as the Candida albicans, Saccharomyces cerevisiae, and Jaminaea angkorensis. To improve quality of the mycelial RNA, we developed an isolation procedure of the hyphal tip, which eliminated the unnecessary vacuoles-containing parts of the hyphae. To increase RNA quantity, we used glass beads in the RNA extraction protocol to achieve stronger breaking of the mycelial walls. All these modifications can also be useful for researchers working with other dimorphic fungi and can contribute to the higher comparability of the transcriptional data coming from yeast-phase cells and hyphae or even from different species.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Papp LA, Ács-Szabó L, Póliska S, Miklós I","authors_abbrev":"Papp LA et al.","canto_added_date":"2021-05-10 08:46:37","canto_curator_role":"JaponicusDB","canto_triage_status":"Method or reagent","citation":"Curr Genet 2021 Oct;67(5):823-830","cv_annotations":{},"doi":"10.1007/s00294-021-01181-4","gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2021","pubmed_entrez_date":"2021-04-10","pubmed_publication_date":"Oct 2021","terms_by_termid":{},"title":"A modified culture medium and hyphae isolation method can increase quality of the RNA extracted from mycelia of a dimorphic fungal species.","uniquename":"PMID:33837814"}


id: PMID:7612397
data:
  {"abstract":"The ultrastructure of isolated cell walls of Schizosaccharomyces pombe was studied by electron microscopy after treatment with the following purified enzymes: endo-beta-(1-->3)-glucanase, endo-beta-(-->6)-glucanase, and endo-alpha-(1-->3)-glucanase produced by Bacillus circulans; exo-beta-(1-->3)-glucanase and endo-beta-(1-->3)-glucanase produced by Schizosaccharomyces japonicus var. versatilis. The exo-beta-(1-->3)-glucanase had no detectable effect on the walls, but amorphous wall material was removed by action of the endo-beta-(1-->3)- and endo-beta-(1-->6)-glucanases of B. circulans to reveal a wall component consisting of densely interwoven microfibrils. The fibrils were hydrolyzed by treatment with the Schiz. japonicus endo-beta-(1-->3)-glucanase followed by B. circulans endo-alpha-(1-->3)-glucanase--suggesting that they were composed of -beta-(1-->3)-linked glucan and alpha-(1-->3)-linked glucan. The presence of a fibrillar component in untreated walls was evident after negative staining.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Kopecká M, Fleet GH, Phaff HJ","authors_abbrev":"Kopecká M et al.","canto_added_date":"2021-05-10 08:46:37","canto_curator_role":"JaponicusDB","canto_triage_status":"Cell composition or WT feature","citation":"J Struct Biol 1995 Mar;114(2):140-52","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"1995","pubmed_entrez_date":"1995-03-01","pubmed_publication_date":"Mar 1995","terms_by_termid":{},"title":"Ultrastructure of the cell wall of Schizosaccharomyces pombe following treatment with various glucanases.","uniquename":"PMID:7612397"}


id: GO_REF:0000064
data:
  {"abstract":"We have created a standard template for classes describing cell components as part of other cell components. The underlying equivalence axiom template is \"P and 'part_of' some W\", where P and W are cell components.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"GO ontology editors","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Representation of cell components as part of other cell components in the Gene Ontology","uniquename":"GO_REF:0000064"}


id: GO_REF:0000065
data:
  {"abstract":"We have created a standard template for classes describing transport of a chemical entity (ChEBI) as a biological process. The underlying equivalence axiom template is \"GO:0006810 and 'transports or maintains localization of' some X\", where X is a chemical entity (CHEBI:24431). The approach to combine GO and ChEBI has been described in the following publication: PMID:23895341.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"GO ontology editors","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Representation of transport of a chemical entity as a biological process in the Gene Ontology","uniquename":"GO_REF:0000065"}


id: PMID:1934083
data:
  {"abstract":"By rhodamine-phalloidin fluorescence, distinct continuous F-actin rings were visualized in 18-20% of the protoplasts of Schizosaccharomyces pombe and S. japonicus var. versatilis, in addition to randomly distributed F-actin dots. Whereas the reversion of ring-lacking protoplasts coincided with the polarization of the dotted F-actin pattern, the ring-containing protoplasts became furrowed as the F-actin rings constricted. The furrowing was more conspicuous in S. japonicus var. versatilis than in S. pombe protoplasts and it was blocked when the reversion was inhibited by Novozyme 234 indicating that the cell wall formation is essential for the F-actin ring constriction.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Jochová J, Rupes I, Streiblová E","authors_abbrev":"Jochová J et al.","canto_added_date":"2021-05-10 08:46:37","canto_curator_role":"JaponicusDB","canto_triage_status":"Cell composition or WT feature","citation":"Cell Biol Int Rep 1991 Jul;15(7):607-10","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"1991","pubmed_entrez_date":"1991-07-01","pubmed_publication_date":"Jul 1991","terms_by_termid":{},"title":"F-actin contractile rings in protoplasts of the yeast Schizosaccharomyces.","uniquename":"PMID:1934083"}


id: PMID:23873862
data:
  {"abstract":"Many fungi respond to light and regulate fungal development and behavior. A blue light-activated complex has been identified in Neurospora crassa as the product of the wc-1 and wc-2 genes. Orthologs of WC-1 and WC-2 have hitherto been found only in filamentous fungi and not in yeast, with the exception of the basidiomycete pathogenic yeast Cryptococcus. Here, we report that the fission yeast Schizosaccharomyces japonicus responds to blue light depending on Wcs1 and Wcs2, orthologs of components of the WC complex. Surprisingly, those of ascomycete S. japonicus are more closely related to those of the basidiomycete. S. japonicus reversibly changes from yeast to hyphae in response to environmental stresses. After incubation at 30°C, a colony of yeast was formed, and then hyphal cells extended from the periphery of the colony. When light cycles were applied, distinct dark- and bright-colored hyphal cell stripes were formed because the growing hyphal cells had synchronously activated cytokinesis. In addition, temperature cycles of 30°C for 12 h and 35°C for 12 h or of 25°C for 12 h and 30°C for 12 h during incubation in the dark induced a response in the hyphal cells similar to that of light. The stripe formation of the temperature cycles was independent of the wcs genes. Both light and temperature, which are daily external cues, have the same effect on growing hyphal cells. A dual sensing mechanism of external cues allows organisms to adapt to daily changes of environmental alteration. ","alleles_by_uniquename":{"SJAG_02860:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_02860","name":"wcs1delta","uniquename":"SJAG_02860:allele-1"},"SJAG_05242:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_05242","name":"wcs2delta","uniquename":"SJAG_05242:allele-1"}},"annotation_curators":[{"annotation_count":7,"community_curator":true,"file_name":null,"file_type":null,"name":"Sho Okamoto","orcid":null}],"annotation_details":{},"annotation_file_curators":[],"approved_date":"2022-12-01","authors":"Okamoto S, Furuya K, Nozaki S, Aoki K, Niki H","authors_abbrev":"Okamoto S et al.","canto_added_date":"2021-05-10 08:46:37","canto_annotation_status":"APPROVED","canto_approved_date":"2022-12-01 10:29:08","canto_approver_orcid":"0000-0002-8138-6851","canto_curator_name":"Sho Okamoto","canto_curator_role":"community","canto_first_approved_date":"2022-12-01 10:29:08","canto_session_key":"0e71b7054e7d7754","canto_session_submitted_date":"2022-12-01 10:28:58","canto_triage_status":"Curatable","citation":"Eukaryot Cell 2013 Sep;12(9):1235-43","cv_annotations":{"biological_process":[{"annotations":[115592,115593],"is_not":false,"summary":null,"term":"GO:0071483"}],"molecular_function":[{"annotations":[115591],"is_not":false,"summary":null,"term":"GO:0009882"}],"single_locus_phenotype":[{"annotations":[115594,115595],"is_not":false,"summary":null,"term":"FYPO:0008031"},{"annotations":[115596,115597],"is_not":false,"summary":null,"term":"FYPO:0008046"}]},"doi":"10.1128/EC.00109-13","gene_count":2,"genes_by_uniquename":{"SJAG_02860":null,"SJAG_05242":null},"genotypes_by_uniquename":{"wcs1delta":{"display_name":"wcs1delta","display_uniquename":"wcs1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02860:allele-1","expression":"Null"}]}]},"wcs2delta":{"display_name":"wcs2delta","display_uniquename":"wcs2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_05242:allele-1","expression":"Null"}]}]}},"ltp_gene_count":2,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2013","pubmed_entrez_date":"2013-07-23","pubmed_publication_date":"Sep 2013","terms_by_termid":{"FYECO:0000005":null,"FYECO:0000126":null,"FYPO:0008031":null,"FYPO:0008046":null,"FYPO_EXT:0000001":null,"GO:0009882":null,"GO:0071483":null},"title":"Synchronous activation of cell division by light or temperature stimuli in the dimorphic yeast Schizosaccharomyces japonicus.","uniquename":"PMID:23873862"}


id: PMID:27697163
data:
  {"abstract":"The present work demonstrates that yeasts belonging to the Schizosaccharomyces genus release a high quantity of polysaccharides of cell wall origin starting from the onset of the alcoholic fermentation. By the end of the alcoholic fermentation, all of the Schizosaccharomyces yeast strains released a quantity of polysaccharides approximately 3-7 times higher than that released by a commercial Saccharomyces cerevisiae yeast strain under the same fermentative conditions of synthetic juice. A higher content of polysaccharide was found in media fermented by Schizosaccharomyces japonicus with respect to that of Schizosaccharomyces pombe. Some of the strains evaluated were also able to produce high levels of pyruvic acid, which has been shown to be an important compound for color stability of wine. The presence of strains with different malic acid consumption patterns along with high polysaccharide release would enable production of naturally modified wines with enhanced mouth feel and reduced acidity. The chemical analysis of the released polysaccharides demonstrated divergence between the two yeast species S. pombe and S. japonicus. A different mannose/galactose ratio and a different percentage of proteins was observed on the polysaccharides released by S. pombe as compared to S. japonicus. Analysis of the proteins released in the media revealed the presence of a glycoprotein with a molecular size around 32-33 kDa only for the species S. japonicus. Mass spectrometry analysis of carbohydrate moieties showed similar proportions among the N-glycan chains released in the media by both yeast species but differences between the two species were also observed. These observations suggest a possible role of rapid MALDI-TOF screening of N-glycans compositional fingerprint as a taxonomic tool for this genus. Polysaccharides release in the media, in particular galactomannoproteins in significant amounts, could make these yeasts particularly interesting also for the industrial production of exogenous polysaccharide preparations.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Domizio P, Liu Y, Bisson LF, Barile D","authors_abbrev":"Domizio P et al.","canto_added_date":"2021-05-10 08:46:37","canto_curator_role":"JaponicusDB","canto_triage_status":"Cell composition or WT feature","citation":"Food Microbiol 2017 Feb;61:136-149","cv_annotations":{},"doi":"10.1016/j.fm.2016.08.010","gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2017","pubmed_entrez_date":"2016-10-05","pubmed_publication_date":"Feb 2017","terms_by_termid":{},"title":"Cell wall polysaccharides released during the alcoholic fermentation by Schizosaccharomyces pombe and S. japonicus: quantification and characterization.","uniquename":"PMID:27697163"}


id: GO_REF:0000054
data:
  {"abstract":"LIFEdb is a database that was created to manage the experimental data produced by the German Cancer Research Institute (DKFZ) and its collaborators, from work on cDNAs contained in the German cDNA Consortium collection. <br>A novel cloning technology was used to rapidly generate N- and C-terminal green fluorescent protein fusions of cDNAs to examine the intracellular localizations of expressed fusion proteins in living cells. GO Cellular Component terms are manually assigned by curators studying fluorescence microscope images of cells labelled with GFP-fused cDNAs. Protein coding regions of novel full length cDNAs are tagged with the coding sequence of the green fluorescent protein, the fusion proteins are then expressed and analyzed for their subcellular localization. <br>Prior to February 2013, all LIFEdb annotations were referenced by PMID: 11256614 (Simpson et al. 2000 EMBO Rep. 1:287-292), a paper describing the protein subcellular localization pilot study and methodology used by LIFEdb. However, it has been decided that these annotations are more correctly described by a GO reference. <br>Resource URL: http://www.dkfz.de/en/mga/Groups/LIFEdb-Database.html <br>Protein subcellular localization images can be viewed on the LIFEdb website, http://www.dkfz.de/gpcf/lifedb.php","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"LIFEdb","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Gene Ontology annotation based on curation of intracellular localizations of expressed fusion proteins in living cells.","uniquename":"GO_REF:0000054"}


id: GO_REF:0000002
data:
  {"abstract":"Transitive assignment of GO terms based on InterPro classification. For any database entry (representing a protein or protein-coding gene) that has been annotated with one or more InterPro domains, the corresponding GO terms are obtained from a translation table of InterPro entries to GO terms (interpro2go) generated manually by the InterPro team at EBI. The mapping file is available at http://www.geneontology.org/external2go/interpro2go.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"DDB, FB, MGI, GOA, ZFIN 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id: PMID:27734801
data:
  {"abstract":"Many eukaryotes assemble a ring-shaped actomyosin network that contracts to drive cytokinesis. Unlike actomyosin in sarcomeres, which cycles through contraction and relaxation, the cytokinetic ring disassembles during contraction through an unknown mechanism. Here we find in  Schizosaccharomyces japonicus  and  Schizosaccharomyces pombe  that, during actomyosin ring contraction, actin filaments associated with actomyosin rings are expelled as micron-scale bundles containing multiple actomyosin ring proteins. Using functional isolated actomyosin rings we show that expulsion of actin bundles does not require continuous presence of cytoplasm. Strikingly, mechanical compression of actomyosin rings results in expulsion of bundles predominantly at regions of high curvature. Our work unprecedentedly reveals that the increased curvature of the ring itself promotes its disassembly. It is likely that such a curvature-induced mechanism may operate in disassembly of other contractile networks.","alleles_by_uniquename":{},"annotation_curators":[{"annotation_count":2,"community_curator":false,"file_name":null,"file_type":null,"name":"Snezhana Oliferenko","orcid":"0000-0002-8138-6851"}],"annotation_details":{},"annotation_file_curators":[],"approved_date":"2021-07-09","authors":"Huang J, Chew TG, Gu Y, Palani S, Kamnev A, Martin DS, Carter NJ, Cross RA, Oliferenko S, Balasubramanian MK","authors_abbrev":"Huang J et al.","canto_added_date":"2021-05-10 08:46:37","canto_annotation_status":"APPROVED","canto_approved_date":"2021-07-09 15:10:46","canto_approver_orcid":"0000-0002-8138-6851","canto_curator_name":"Snezhana Oliferenko","canto_curator_role":"JaponicusDB","canto_first_approved_date":"2021-07-09 15:10:46","canto_session_key":"f47401f00ac444e0","canto_session_submitted_date":"2021-07-09 15:10:39","canto_triage_status":"Curatable","citation":"Elife 2016 10 13;5","cv_annotations":{"cellular_component":[{"annotations":[115326],"is_not":false,"summary":null,"term":"GO:0032432"},{"annotations":[115325],"is_not":false,"summary":null,"term":"GO:0005826"}]},"doi":"10.7554/eLife.21383","gene_count":1,"genes_by_uniquename":{"SJAG_04887":null},"genotypes_by_uniquename":{},"ltp_gene_count":1,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2016","pubmed_entrez_date":"2016-10-14","pubmed_publication_date":"13 10 2016","terms_by_termid":{"GO:0005826":null,"GO:0032432":null},"title":"Curvature-induced expulsion of actomyosin bundles during cytokinetic ring contraction.","uniquename":"PMID:27734801"}


id: PMID:24699070
data:
  {"abstract":"The post-cytokinetic separation of cells in cell-walled organisms involves enzymic processes that degrade a specific layer of the division septum and the region of the mother cell wall that edges the septum. In the fission yeast Schizosaccharomyces pombe, the 1,3-α-glucanase Agn1p, originally identified as a mutanase-like glycoside hydrolase family 71 (GH71) enzyme, dissolves the mother cell wall around the septum edge. Our search in the genomes of completely sequenced fungi identified GH71 hydrolases in Basidiomycota, Taphrinomycotina and Pezizomycotina, but not in Saccharomycotina. The most likely Agn1p orthologues in Pezizomycotina species are not mutanases having mutanase-binding domains, but experimentally non-characterized hypothetical proteins that have no carbohydrate-binding domains. The analysis of the GH71 domains corroborated the phylogenetic relationships of the Schizosaccharomyces species determined by previous studies, but suggested a closer relationship to the Basidiomycota proteins than to the Ascomycota proteins. In the Schizosaccharomyces genus, the Agn1p proteins are structurally conserved: their GH71 domains are flanked by N-terminal secretion signals and C-terminal sequences containing the conserved block YNFNA(Y)/HTG. The inactivation of the agn1(Sj) gene in Schizosaccharomyces japonicus, the only true dimorphic member of the genus, caused a severe cell-separation defect in its yeast phase, but had no effect on the hyphal growth and yeast-to-mycelium transition. It did not affect the mycelium-to-yeast transition either, only delaying the separation of the yeast cells arising from the fragmenting hyphae. The heterologous expression of agn1(Sj) partially rescued the separation defect of the agn1Δ cells of Schizosaccharomyces pombe. The results presented indicate that the fission yeast Agn1p 1,3-α-glucanases of Schizosaccharomyces japonicus and Schizosaccharomyces pombe share conserved functions in the yeast phase. ","alleles_by_uniquename":{"SJAG_03444:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_03444","name":"agn1delta","uniquename":"SJAG_03444:allele-1"}},"annotation_curators":[{"annotation_count":1,"community_curator":false,"file_name":null,"file_type":null,"name":"Snezhana Oliferenko","orcid":"0000-0002-8138-6851"},{"annotation_count":1,"community_curator":false,"file_name":null,"file_type":null,"name":"Val Wood","orcid":"0000-0001-6330-7526"}],"annotation_details":{},"annotation_file_curators":[],"approved_date":"2021-07-09","authors":"Sipiczki M, Balazs A, Monus A, Papp L, Horvath A, Sveiczer A, Miklos I","authors_abbrev":"Sipiczki M et al.","canto_added_date":"2021-05-10 08:46:37","canto_annotation_status":"APPROVED","canto_approved_date":"2021-09-01 07:35:20","canto_approver_orcid":"0000-0001-6330-7526","canto_curator_name":"Snezhana Oliferenko","canto_curator_role":"JaponicusDB","canto_first_approved_date":"2021-07-09 13:16:43","canto_session_key":"b8bc1733e6f2c600","canto_session_submitted_date":"2021-07-09 13:16:35","canto_triage_status":"Curatable","citation":"Microbiology (Reading) 2014 Jun;160(Pt 6):1063-1074","cv_annotations":{"biological_process":[{"annotations":[115107],"is_not":false,"summary":null,"term":"GO:0030995"}],"single_locus_phenotype":[{"annotations":[115106],"is_not":false,"summary":null,"term":"FYPO:0001971"}]},"doi":"10.1099/mic.0.077511-0","gene_count":1,"genes_by_uniquename":{"SJAG_03444":null},"genotypes_by_uniquename":{"agn1delta":{"display_name":"agn1delta","display_uniquename":"agn1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03444:allele-1","expression":"Null"}]}]}},"ltp_gene_count":1,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2014","pubmed_entrez_date":"2014-04-05","pubmed_publication_date":"Jun 2014","terms_by_termid":{"FYPO:0001971":null,"GO:0030995":null},"title":"Phylogenetic and comparative functional analysis of the cell-separation α-glucanase Agn1p in Schizosaccharomyces.","uniquename":"PMID:24699070"}


id: PMID:23150598
data:
  {"abstract":"A key question in developmental biology addresses the mechanism of asymmetric cell division. Asymmetry is crucial for generating cellular diversity required for development in multicellular organisms. As one of the potential mechanisms, chromosomally borne epigenetic difference between sister cells that changes mating/cell type has been demonstrated only in the Schizosaccharomyces pombe fission yeast. For technical reasons, it is nearly impossible to determine the existence of such a mechanism operating during embryonic development of multicellular organisms. Our work addresses whether such an epigenetic mechanism causes asymmetric cell division in the recently sequenced fission yeast, S. japonicus (with 36% GC content), which is highly diverged from the well-studied S. pombe species (with 44% GC content). We find that the genomic location and DNA sequences of the mating-type loci of S. japonicus differ vastly from those of the S. pombe species. Remarkably however, similar to S. pombe, the S. japonicus cells switch cell/mating type after undergoing two consecutive cycles of asymmetric cell divisions: only one among four \"granddaughter\" cells switches. The DNA-strand-specific epigenetic imprint at the mating-type locus1 initiates the recombination event, which is required for cellular differentiation. Therefore the S. pombe and S. japonicus mating systems provide the first two examples in which the intrinsic chirality of double helical structure of DNA forms the primary determinant of asymmetric cell division. Our results show that this unique strand-specific imprinting/segregation epigenetic mechanism for asymmetric cell division is evolutionary conserved. Motivated by these findings, we speculate that DNA-strand-specific epigenetic mechanisms might have evolved to dictate asymmetric cell division in diploid, higher eukaryotes as well.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Yu C, Bonaduce MJ, Klar AJ","authors_abbrev":"Yu C et al.","canto_added_date":"2021-05-10 08:46:37","canto_curator_role":"JaponicusDB","canto_triage_status":"Cell composition or WT feature","citation":"Genetics 2013 Jan;193(1):85-94","cv_annotations":{},"doi":"10.1534/genetics.112.146233","gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2013","pubmed_entrez_date":"2012-11-15","pubmed_publication_date":"Jan 2013","terms_by_termid":{},"title":"Defining the epigenetic mechanism of asymmetric cell division of Schizosaccharomyces japonicus yeast.","uniquename":"PMID:23150598"}


id: PMID:27059155
data:
  {"abstract":"In the fission yeast Schizosaccharomyces pombe (Sp), Mid1/Dmf1 plays an important role in positioning the division site by inducing formation of the contractile ring (CR). Mid1, emanating from the nucleus located in the cell center, forms a dozen of nodes in the middle cell cortex ahead of mitosis, and actin filaments and myosin II accumulated at each node interact and assemble the CR in metaphase. Curiously, in another fission yeast S. japonicus (Sj), CR formation begins after nuclear segregation in late anaphase. Here, we investigated the role of S. japonicus Mid1 during mitosis to compare the molecular mechanisms that determine the cell division site in Schizosaccharomyces. Similar to Sp Mid1, Sj Mid1 often accumulated in the nucleus of interphase cells. Moreover, Sj Mid1 localized to cortical dots with myosin II in the future division site and formed a medial ring in mitotic cells. However, S. japonicus cells without Mid1 function still carried out symmetrical binary division. Therefore, the Mid1 dependency for positional control of the cell division site is possibly different between the two species. Meanwhile, we found that Sj Mid1 enhanced CR formation, in a manner possibly similar to that by Sp Mid1. ","alleles_by_uniquename":{"SJAG_01143:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_01143","name":"mid1delta","uniquename":"SJAG_01143:allele-1"}},"annotation_curators":[{"annotation_count":3,"community_curator":false,"file_name":null,"file_type":null,"name":"Snezhana Oliferenko","orcid":"0000-0002-8138-6851"},{"annotation_count":1,"community_curator":false,"file_name":null,"file_type":null,"name":"Val Wood","orcid":"0000-0001-6330-7526"}],"annotation_details":{},"annotation_file_curators":[],"approved_date":"2021-07-28","authors":"Yasuda T, Takaine M, Numata O, Nakano K","authors_abbrev":"Yasuda T et al.","canto_added_date":"2021-05-10 08:46:37","canto_annotation_status":"APPROVED","canto_approved_date":"2021-09-01 07:07:40","canto_approver_orcid":"0000-0001-6330-7526","canto_curator_name":"Snezhana Oliferenko","canto_curator_role":"JaponicusDB","canto_first_approved_date":"2021-07-28 11:44:06","canto_session_key":"f86427f99db0ccf7","canto_session_submitted_date":"2021-07-28 11:44:00","canto_triage_status":"Curatable","citation":"Genes Cells 2016 Jun;21(6):594-607","cv_annotations":{"biological_process":[{"annotations":[115429],"is_not":false,"summary":null,"term":"GO:1903475"}],"cellular_component":[{"annotations":[115426],"is_not":false,"summary":null,"term":"GO:0005826"},{"annotations":[115427],"is_not":false,"summary":null,"term":"GO:1990753"}],"single_locus_phenotype":[{"annotations":[115428],"is_not":false,"summary":null,"term":"FYPO:0000729"}]},"doi":"10.1111/gtc.12368","gene_count":1,"genes_by_uniquename":{"SJAG_01143":null},"genotypes_by_uniquename":{"mid1delta":{"display_name":"mid1delta","display_uniquename":"mid1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_01143:allele-1","expression":"Null"}]}]}},"ltp_gene_count":1,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2016","pubmed_entrez_date":"2016-04-10","pubmed_publication_date":"Jun 2016","terms_by_termid":{"FYPO:0000729":null,"GO:0005826":null,"GO:0051329":null,"GO:1903475":null,"GO:1990753":null},"title":"Anillin-related protein Mid1 regulates timely formation of the contractile ring in the fission yeast Schizosaccharomyces japonicus.","uniquename":"PMID:27059155"}


id: PMID:12527786
data:
  {"abstract":"The fission yeasts are members of the fungal order Schizosaccharomycetales, a candidate deep-diverging group within Ascomycota. Although a great deal of molecular information is available from Schizosaccharomyces pombe, a model eukaryote, very little is available from other members of this group. In order to better characterize mitochondrial genome evolution in this fungal lineage, the mitochondrial DNA (mtDNA) of two additional fission yeasts, Schizosaccharomyces octosporus and Schizosaccharomyces japonicus var. japonicus, was sequenced. Whereas the mtDNA of S.pombe is only 19 431 bp, the mtDNA of S.octosporus is 44 227 bp, and that of S.japonicus var. japonicus is over 80 kb. The size variation of these mtDNAs is due largely to non-coding regions. The gene content in the latter two mtDNAs is almost identical to that of the completely sequenced S.pombe mtDNA, which encodes 25 tRNA species, the large and small mitochondrial ribosomal RNAs (rnl and rns), the RNA component of mitochondrial RNaseP (rnpB), mitochondrial small subunit ribosomal protein 3 (rps3), cytochrome oxidase subunits 1, 2 and 3 (cox1, cox2 and cox3) and ATP-synthase subunits 6, 8 and 9 (atp6, atp8 and atp9). However, trnI2(cau) (C modified to lysidine) is absent in the S.octosporus mtDNA, as are corresponding ATA codons in its protein-coding genes, and rps3 and rnpB are not found in the mtDNA of S.japonicus var. japonicus. The mtDNA of S.octosporus contains five double hairpin elements, the first report of these elements in an ascomycete. This study provides further evidence in favor of the mobility of these elements, and supports their role in mitochondrial genome rearrangement. The results of our phylogenetic analysis support the monophyly of the Schizosaccharomycetales, but question their grouping within the Archiascomycota.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Bullerwell CE, Leigh J, Forget L, Lang BF","authors_abbrev":"Bullerwell CE et al.","canto_added_date":"2021-05-10 08:46:37","canto_curator_role":"JaponicusDB","canto_triage_status":"Sequence feature or region","citation":"Nucleic Acids Res 2003 Jan 15;31(2):759-68","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2003","pubmed_entrez_date":"2003-01-16","pubmed_publication_date":"15 Jan 2003","terms_by_termid":{},"title":"A comparison of three fission yeast mitochondrial genomes.","uniquename":"PMID:12527786"}


id: PMID:22641476
data:
  {"abstract":"Measuring relative genetic distances is one of the best ways to locate genetic loci. Here we report the construction of a strains set for genetic mapping in Schizosaccharomyces japonicus, which belongs to the genus Schizosaccharomyces together with the well-studied fission yeast Sz. pombe. We constructed 29 strains that bear a positive-negative selection marker at different loci. The marker was inserted every 500 kb in the genome of Sz. japonicus. Each marker thus becomes a 'scale mark' of a chromosome that behaves like a yardstick. By determining the genetic distances from the inserted markers, the relative location of a genomic mutation can be determined. We also constructed a fosmid library that covers an entire genome of Sz. japonicus. These tools together would facilitate identification and cloning of the gene.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Furuya K, Aoki K, Niki H","authors_abbrev":"Furuya K et al.","canto_added_date":"2021-05-10 08:46:37","canto_curator_role":"JaponicusDB","canto_triage_status":"Method or reagent","citation":"Yeast 2012 Jun;29(6):241-9","cv_annotations":{},"doi":"10.1002/yea.2907","gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2012","pubmed_entrez_date":"2012-05-30","pubmed_publication_date":"Jun 2012","terms_by_termid":{},"title":"Construction of an insertion marker collection of Sz. japonicus (IMACS) for genetic mapping and a fosmid library covering its genome.","uniquename":"PMID:22641476"}


id: PMID:30726171
data:
  {"abstract":"Many fungal species are dimorphic, exhibiting both unicellular yeast-like and filamentous forms. Schizosaccharomyces japonicus, a member of the fission yeast clade, is one such dimorphic fungus. Here, we first identify fruit extracts as natural, stress-free, starvation-independent inducers of filamentation, which we use to describe the properties of the dimorphic switch. During the yeast-to-hypha transition, the cell evolves from a bipolar to a unipolar system with 10-fold accelerated polarized growth but constant width, vacuoles segregated to the nongrowing half of the cell, and hyper-lengthening of the cell. We demonstrate unusual features of S. japonicus hyphae: these cells lack a Spitzenkörper, a vesicle distribution center at the hyphal tip, but display more rapid cytoskeleton-based transport than the yeast form, with actin cables being essential for the transition. S. japonicus hyphae also remain mononuclear and undergo complete cell divisions, which are highly asymmetric: one daughter cell inherits the vacuole, the other the growing tip. We show that these elongated cells scale their nuclear size, spindle length, and elongation rates, but display altered division size controls. This establishes S. japonicus as a unique system that switches between symmetric and asymmetric modes of growth and division.","alleles_by_uniquename":{"SJAG_02392:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_02392","name":"pom1delta","uniquename":"SJAG_02392:allele-1"},"SJAG_02695:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_02695","name":"tip1delta","uniquename":"SJAG_02695:allele-1"},"SJAG_04703:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_04703","name":"for3delta","uniquename":"SJAG_04703:allele-1"}},"annotation_curators":[{"annotation_count":21,"community_curator":true,"file_name":null,"file_type":null,"name":"Sophie Martin","orcid":null}],"annotation_details":{},"annotation_file_curators":[],"approved_date":"2021-06-16","authors":"Kinnaer C, Dudin O, Martin SG","authors_abbrev":"Kinnaer C et al.","canto_added_date":"2021-05-10 08:46:37","canto_annotation_status":"APPROVED","canto_approved_date":"2021-06-16 18:49:31","canto_approver_orcid":"0000-0002-8138-6851","canto_curator_name":"Sophie Martin","canto_curator_role":"community","canto_first_approved_date":"2021-06-16 18:49:31","canto_session_key":"bf05a60cb09b4cdb","canto_session_submitted_date":"2021-06-04 10:25:08","canto_triage_status":"Curatable","citation":"Mol Biol Cell 2019 04 01;30(8):975-991","cv_annotations":{"cellular_component":[{"annotations":[115297],"is_not":false,"summary":null,"term":"GO:0051286"},{"annotations":[115279,115281,115283,115285,115287,115289],"is_not":false,"summary":null,"term":"GO:0035838"},{"annotations":[115298],"is_not":false,"summary":null,"term":"GO:0085039"},{"annotations":[115280,115282,115284,115286,115288,115290],"is_not":false,"summary":null,"term":"GO:0001411"},{"annotations":[115291],"is_not":false,"summary":null,"term":"GO:0035371"},{"annotations":[115292],"is_not":false,"summary":null,"term":"GO:0030133"}],"single_locus_phenotype":[{"annotations":[115294],"is_not":false,"summary":null,"term":"FYPO:0003315"},{"annotations":[115295],"is_not":false,"summary":null,"term":"FYPO:0001355"},{"annotations":[115299],"is_not":false,"summary":null,"term":"FYPO:0007801"},{"annotations":[115296],"is_not":false,"summary":null,"term":"FYPO:0004293"},{"annotations":[115293],"is_not":false,"summary":null,"term":"FYPO:0001956"}]},"doi":"10.1091/mbc.E18-12-0774","gene_count":9,"genes_by_uniquename":{"SJAG_01196":null,"SJAG_02392":null,"SJAG_02695":null,"SJAG_03011":null,"SJAG_03625":null,"SJAG_03915":null,"SJAG_04624":null,"SJAG_04703":null,"SJAG_04960":null},"genotypes_by_uniquename":{"for3delta":{"display_name":"for3delta","display_uniquename":"for3delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04703:allele-1","expression":"Null"}]}]},"pom1delta":{"display_name":"pom1delta","display_uniquename":"pom1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02392:allele-1","expression":"Null"}]}]},"tip1delta":{"display_name":"tip1delta","display_uniquename":"tip1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02695:allele-1","expression":"Null"}]}]}},"ltp_gene_count":9,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2019","pubmed_entrez_date":"2019-02-07","pubmed_publication_date":"01 04 2019","terms_by_termid":{"FYPO:0001355":null,"FYPO:0001956":null,"FYPO:0003315":null,"FYPO:0004293":null,"FYPO:0007801":null,"GO:0001411":null,"GO:0030133":null,"GO:0035371":null,"GO:0035838":null,"GO:0051286":null,"GO:0085039":null},"title":"Yeast-to-hypha transition of Schizosaccharomyces japonicus in response to environmental stimuli.","uniquename":"PMID:30726171"}


id: PMID:36058798
data:
  {"abstract":"Schizosaccharomyces japonicus is a dimorphic yeast, transiting between unicellular and hyphal growth. The glycoproteins of fission yeast contain, in addition to mannose (Man), a large number of galactose (Gal) residues. Previously, we reported that the cell-surface O-glycans of S. japonicus comprise mainly tri-saccharides (Gal-Man-Man) as a main component, in contrast to the tetra-saccharides observed in other Schizosaccharomyces species. Here we have investigated the function of cell-surface Gal residues in S. japonicus. Because disruption of gms1 + , encoding the UDP-Gal transporter required for galactomannan synthesis, abolishes cell-surface galactosylation in Schizosaccharomyces pombe, we constructed a deletion mutant of the homologous gene in S. japonicus gms1Δ [gms1 (S.j)] and determined the N- and O-linked oligosaccharide structures present on the cell surface. Disruption of gms1 (S.j) resulted in a complete lack of Gal on the cell surface, indicating that Gms1 plays an essential role in supplying UDP-Gal from the cytoplasm to the Golgi lumen. Analytical microscopy of gms1Δ demonstrated that the lack of cell-surface Gal did not affect cell growth or morphology during vegetative growth. However, hyphal development was blocked in gms1Δ, even in the presence of the topoisomerase I inhibitor camptothecin, which is known to induce hyphal differentiation in wild-type S. japonicus. Collectively, these findings show that Gal-containing oligosaccharides are required for cell wall integrity during filamentous growth in S. japonicus.","alleles_by_uniquename":{"SJAG_04697:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_04697","name":"gms1delta","uniquename":"SJAG_04697:allele-1"}},"annotation_curators":[{"annotation_count":6,"community_curator":false,"file_name":null,"file_type":null,"name":"Snezhana Oliferenko","orcid":"0000-0002-8138-6851"}],"annotation_details":{},"annotation_file_curators":[],"approved_date":"2022-12-01","authors":"Fukunaga T, Ohashi T, Tanaka Y, Yoshimatsu T, Higuchi Y, Maekawa H, Takegawa K","authors_abbrev":"Fukunaga T et al.","canto_added_date":"2022-11-25 12:12:27","canto_annotation_status":"APPROVED","canto_approved_date":"2022-12-01 10:35:53","canto_approver_orcid":"0000-0002-8138-6851","canto_curator_name":"Snezhana Oliferenko","canto_curator_role":"JaponicusDB","canto_first_approved_date":"2022-12-01 10:35:53","canto_session_key":"465355706dc223a3","canto_session_submitted_date":"2022-11-25 12:49:07","canto_triage_status":"Curatable","citation":"J Biosci Bioeng 2022 Nov;134(5):384-392","cv_annotations":{"single_locus_phenotype":[{"annotations":[115151],"is_not":false,"summary":null,"term":"FYPO:0008029"},{"annotations":[115147],"is_not":false,"summary":null,"term":"FYPO:0000082"},{"annotations":[115150],"is_not":false,"summary":null,"term":"FYPO:0007776"},{"annotations":[115149],"is_not":false,"summary":null,"term":"FYPO:0007780"},{"annotations":[115146],"is_not":false,"summary":null,"term":"FYPO:0003727"},{"annotations":[115148],"is_not":false,"summary":null,"term":"FYPO:0000106"}]},"doi":"10.1016/j.jbiosc.2022.07.014","gene_count":1,"genes_by_uniquename":{"SJAG_04697":null},"genotypes_by_uniquename":{"gms1delta":{"display_name":"gms1delta","display_uniquename":"gms1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_04697:allele-1","expression":"Null"}]}]}},"ltp_gene_count":1,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2022","pubmed_entrez_date":"2022-09-04","pubmed_publication_date":"Nov 2022","terms_by_termid":{"FYECO:0000004":null,"FYECO:0000005":null,"FYECO:0000126":null,"FYECO:0000137":null,"FYECO:0000314":null,"FYPO:0000082":null,"FYPO:0000106":null,"FYPO:0003727":null,"FYPO:0007776":null,"FYPO:0007780":null,"FYPO:0008029":null,"FYPO_EXT:0000001":null,"FYPO_EXT:0000002":null},"title":"Galactosylation of cell-surface glycoprotein required for hyphal growth and cell wall integrity in Schizosaccharomyces japonicus.","uniquename":"PMID:36058798"}


id: GO_REF:0000047
data:
  {"abstract":"This describes a method for supplying a NOT-qualified, IKR-evidenced GO annotation to a gene product, when general sequence homology considerations would suggest a function or location, or a role in a biological process, but where a curator has determined that the absence of key sequence residues, known to be required for an expected activity or location, indicating the gene product is unlikely to be able to carry out the implied activity, involvement in a process or cellular component location. This reference should only be used used when an IKR-evidenced annotation is made based on curator judgement from manually reviewing the sequence of the gene product and where no publication can be found to support the curators conclusion. It is preferable to cite a peer-reviewed publication (such as a PubMed identifier) for IKR-evidenced annotations whenever possible. Curators will have carefully reviewed the sequence of the annotated protein, and established that the key residues known to be required for an expected activity or location are not present. Inclusion of an identifier in the 'with/from' field, that highlights to the user the lacking residues(e.g. an alignment, domain or rule identifier) is absolutely required when annotating to IKR with this GO_REF. Documentation on the GOC website provides more details on the <a href = \"http://www.geneontology.org/GO.evidence.shtml#ikr\">correct use of the IKR evidence code</a>.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"GO curators","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Gene Ontology annotation based on absence of key sequence residues.","uniquename":"GO_REF:0000047"}


id: GO_REF:0000087
data:
  {"abstract":"We have created a standard template for classes describing the protein localization and establishment of protein localization to a cellular component as a biological process. The underlying equivalence axiom templates are \"GO:0008104 and 'has_target_end_location' some C\" (protein localization) and \"GO:0045184 and 'has_target_end_location' some C\" (establishment of protein localization), where C is cellular component.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"GO ontology editors","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Representation of protein localization and establishment of protein localization as biological process in the Gene Ontology","uniquename":"GO_REF:0000087"}


id: PMID:22586155
data:
  {"abstract":"tRNA genes are interspersed throughout eukaryotic DNA, contributing to genome architecture and evolution in addition to translation of the transcriptome. Codon use correlates with tRNA gene copy number in noncomplex organisms including yeasts. Synonymous codons impact translation with various outcomes, dependent on relative tRNA abundances. Availability of whole-genome sequences allowed us to examine tRNA gene copy number variation (tgCNV) and codon use in four Schizosaccharomyces species and Saccharomyces cerevisiae. tRNA gene numbers vary from 171 to 322 in the four Schizosaccharomyces despite very high similarity in other features of their genomes. In addition, we performed whole-genome sequencing of several related laboratory strains of Schizosaccharomyces pombe and found tgCNV at a cluster of tRNA genes. We examined for the first time effects of wobble rules on correlation of tRNA gene number and codon use and showed improvement for S. cerevisiae and three of the Schizosaccharomyces species. In contrast, correlation in Schizosaccharomyces japonicus is poor due to markedly divergent tRNA gene content, and much worsened by the wobble rules. In japonicus, some tRNA iso-acceptor genes are absent and others are greatly reduced relative to the other yeasts, while genes for synonymous wobble iso-acceptors are amplified, indicating wobble use not apparent in any other eukaryote. We identified a subset of japonicus-specific wobbles that improves correlation of codon use and tRNA gene content in japonicus. We conclude that tgCNV is high among Schizo species and occurs in related laboratory strains of S. pombe (and expectedly other species), and tRNAome-codon analyses can provide insight into species-specific wobble decoding.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Iben JR, Maraia RJ","authors_abbrev":"Iben JR et al.","canto_added_date":"2021-05-10 08:46:37","canto_curator_role":"JaponicusDB","canto_triage_status":"Sequence feature or region","citation":"RNA 2012 Jul;18(7):1358-72","cv_annotations":{},"doi":"10.1261/rna.032151.111","gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2012","pubmed_entrez_date":"2012-05-16","pubmed_publication_date":"Jul 2012","terms_by_termid":{},"title":"tRNAomics: tRNA gene copy number variation and codon use provide bioinformatic evidence of a new anticodon:codon wobble pair in a eukaryote.","uniquename":"PMID:22586155"}


id: PMID:37849458
data:
  {"abstract":"We previously demonstrated that we could hijack the fungal pheromone signaling pathway to provide a living yeast biosensor where peptide biomarkers were recognized by G-protein-coupled receptors and engineered to transcribe a readout. Here, we demonstrated that the protease could be reintroduced to the biosensor to provide a simple mechanism for distinguishing single-amino-acid changes in peptide ligands that, otherwise, would likely be difficult to detect using binding-based assays. We characterized the dose-response curves for five fungal pheromone G-protein-coupled receptors, peptides, and proteases -Saccharomyces cerevisiae ,  Candida albicans ,  Schizosaccharomyces pombe ,  Schizosaccharomyces octosporus , and  Schizosaccharomyces japonicus . Alanine scanning was carried out for the most selective of these- S. cerevisiae  and  C. albicans -with and without the protease. Two peptide variants were discovered, which showed diminished cleavage by the protease (CaPep2A and CaPep2A13A). Those peptides were then distinguished by utilizing the biosensor strains with and without the protease, which selectively cleaved and altered the apparent concentration of peptide required for half-maximal activation for 2 peptides-CaPep and CaPep13A, respectively-by more than one order of magnitude. These results support the hypothesis that the living yeast biosensor with a sequence-specific protease can translate single-amino-acid changes into more than one order of magnitude apparent shift in the concentration of peptide required for half-maximal activation. With further engineering by computational modeling and directed evolution, the biosensor could likely distinguish a wide variety of peptide sequences beyond the alanine scanning carried out here. In the future, we envision incorporating proteases into our living yeast biosensor for use as a point of care diagnostic, a scalable communication language, and other applications.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Crnković T, Bokor BJ, Lockwood ME, Cornish VW","authors_abbrev":"Crnković T et al.","canto_added_date":"2023-10-19 00:40:03","canto_curator_role":"JaponicusDB","canto_triage_status":"Method or reagent","citation":"Biodes Res 2023;5:0003","cv_annotations":{},"doi":"10.34133/bdr.0003","gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2023","pubmed_entrez_date":"2023-10-18","pubmed_publication_date":"2023","terms_by_termid":{},"title":"Peptide Variant Detection by a Living Yeast Biosensor via an Epitope-Selective Protease.","uniquename":"PMID:37849458"}


id: GO_REF:0000094
data:
  {"abstract":"We have created a standard template for classes describing the development of a metazoan structure as a biological process. The underlying equivalence axiom template is \"'anatomical structure development' and 'results in development of' some E\", where E is a anatomical entity (UBERON:0001062).","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"GO ontology editors","canto_curator_role":"JaponicusDB","cv_annotations":{},"gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"terms_by_termid":{},"title":"Representation of metazoan development as biological process in the Gene Ontology","uniquename":"GO_REF:0000094"}


id: PMID:35359202
data:
  {"abstract":"The fission yeast genus Schizosaccharomyces contains important model organisms for biological research. In particular, S. pombe is a widely used model eukaryote. So far little is known about the natural and artificial habitats of species in this genus. Finding out where S. pombe and other fission yeast species occur and how they live in their habitats can promote better understanding of their biology. Here we investigate in which substrates S. pombe, S. octosporus, S. osmophilus and S. japonicus are present. To this end about 2100 samples consisting of soil, tree sap fluxes, fresh fruit, dried fruit, honey, cacao beans, molasses and other substrates were analyzed. Effective isolation methods that allow efficient isolation of the above mentioned species were developed. Based on the frequency of isolating different fission yeast species in various substrates and on extensive literature survey, conclusions are drawn on their ecology. The results suggest that the primary habitat of S. pombe and S. octosporus is honeybee honey. Both species were also frequently detected on certain dried fruit like raisins, mango or pineapple to which they could be brought by the honey bees during ripening or during drying. While S. pombe was regularly isolated from grape mash and from fermented raw cacao beans S. octosporus was never isolated from fresh fruit. The main habitat of S. osmophilus seems to be solitary bee beebread. It was rarely isolated from raisins. S. japonicus was mainly found in forest substrates although it occurs on fruit and in fruit fermentations, too.","alleles_by_uniquename":{},"annotation_curators":[],"annotation_details":{},"annotation_file_curators":[],"authors":"Brysch-Herzberg M, Jia GS, Seidel M, Assali I, Du LL","authors_abbrev":"Brysch-Herzberg M et al.","canto_added_date":"2023-05-04 00:40:04","canto_curator_role":"JaponicusDB","canto_triage_status":"Phylogeny and evolutionary studies","citation":"Antonie Van Leeuwenhoek 2022 May;115(5):661-695","cv_annotations":{},"doi":"10.1007/s10482-022-01720-0","gene_count":0,"genes_by_uniquename":{},"genotypes_by_uniquename":{},"ltp_gene_count":0,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2022","pubmed_entrez_date":"2022-04-01","pubmed_publication_date":"May 2022","terms_by_termid":{},"title":"Insights into the ecology of Schizosaccharomyces species in natural and artificial habitats.","uniquename":"PMID:35359202"}


id: PMID:22300943
data:
  {"abstract":"The fission yeasts (Schizosaccharomyces) representing a highly divergent phylogenetic branch of Fungi evolved from filamentous ancestors by gradual transition from mycelial growth to yeast morphology. For the transition, a mechanism had been developed that separates the sister cells after the completion of cytokinesis. Numerous components of the separation mechanism have been characterised in Schizosaccharomycespombe, including the zinc-finger transcription factor Ace2p and the fork-head transcription factor Sep1p. Here we show that both regulators have regions conserved within the genus. The most conserved parts contain the DNA-binding domains whose amino-acid sequences perfectly reflect the phylogenetic positions of the species. The less conserved parts of the proteins contain sequence blocks specific for the whole genus or only for the species propagating predominantly or exclusively as yeasts. Inactivation of either gene in the dimorphic species Schizosaccharomycesjaponicus abolished cell separation in the yeast phase conferring hypha-like morphology but did not change the growth pattern to unipolar and did not cause extensive polar vacuolation characteristic of the true mycelium. Neither mutation affected the mycelial phase, but both mutations hampered the hyphal fragmentation at the mycelium-to-yeast transition. Ace2p(Sj) acts downstream of Sep1p(Sj) and regulates the orthologues of the Ace2p-dependent S.pombe genes agn1(+) (1,3-alpha-glucanase) and eng1(+) (1,3-beta-glucanase) but does not regulate the orthologue of cfh4(+) (chitin synthase regulatory factor). These results and the complementation of the cell separation defects of the ace2(-) and sep1(-) mutations of S.pombe by heterologously expressed ace2(Sj) and sep1(Sj) indicate that the cell separation mechanism is conserved in the Schizosaccharomyces genus.","alleles_by_uniquename":{"SJAG_02252:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_02252","name":"ace2delta","uniquename":"SJAG_02252:allele-1"},"SJAG_03143:allele-1":{"allele_type":"deletion","description":"deletion","gene_uniquename":"SJAG_03143","name":"sep1delta","uniquename":"SJAG_03143:allele-1"}},"annotation_curators":[{"annotation_count":7,"community_curator":false,"file_name":null,"file_type":null,"name":"Snezhana Oliferenko","orcid":"0000-0002-8138-6851"}],"annotation_details":{},"annotation_file_curators":[],"approved_date":"2022-12-09","authors":"Balazs A, Batta G, Miklos I, Acs-Szabo L, Vazquez de Aldana CR, Sipiczki M","authors_abbrev":"Balazs A et al.","canto_added_date":"2022-12-09 17:19:01","canto_annotation_status":"APPROVED","canto_approved_date":"2022-12-09 17:39:12","canto_approver_orcid":"0000-0002-8138-6851","canto_curator_name":"Snezhana Oliferenko","canto_curator_role":"JaponicusDB","canto_first_approved_date":"2022-12-09 17:39:12","canto_session_key":"f106e12e6366fbad","canto_session_submitted_date":"2022-12-09 17:38:34","canto_triage_status":"Curatable","citation":"Fungal Genet Biol 2012 Mar;49(3):235-49","cv_annotations":{"single_locus_phenotype":[{"annotations":[115371,115372],"is_not":false,"summary":null,"term":"FYPO:0006557"}]},"doi":"10.1016/j.fgb.2012.01.003","gene_count":2,"genes_by_uniquename":{"SJAG_02252":null,"SJAG_03143":null},"genotypes_by_uniquename":{"ace2delta":{"display_name":"ace2delta","display_uniquename":"ace2delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_02252:allele-1","expression":"Null"}]}]},"sep1delta":{"display_name":"sep1delta","display_uniquename":"sep1delta","loci":[{"expressed_alleles":[{"allele_uniquename":"SJAG_03143:allele-1","expression":"Null"}]}]}},"ltp_gene_count":2,"ortholog_annotations":[],"paralog_annotations":[],"physical_interactions":[],"publication_year":"2012","pubmed_entrez_date":"2012-02-04","pubmed_publication_date":"Mar 2012","terms_by_termid":{"FYECO:0000137":null,"FYPO:0006557":null,"FYPO_EXT:0000001":null,"FYPO_EXT:0000002":null},"title":"Conserved regulators of the cell separation process in Schizosaccharomyces.","uniquename":"PMID:22300943"}


