wiki:AntoniasCuratedPapers

Version 590 (modified by antonialock, 9 years ago) (diff)

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https://sourceforge.net/apps/trac/pombase/wiki/CuratorTrainingPapers

Papers needing ODA conversions
Papers with WT info or no annotations


21745468Antonia
lkh1, dsk1((phenotype)) slow cell growth (cell growth assay)allele=Δdsk1Δlkh1
lkh1, dsk1((phenotype)) slow cell growth (cell growth assay)allele=Δdsk1,lkh1-K391A


7900424
mfm1/2/3triple deletion, sporulation abolisheddiploid
mfm1/2/3triple deletion, normal sporulationdiploid, M-factor added
mfm1/2/3triple deletion, normal sporulationdiploid, P-factor added
mfm1/2/3triple deletion, decreased protein levelhas regulation target(Pfactor), diploid


17369611Antonia
rtt109Delta rtt109/Histone H3 K56R double-mutant phenotypeasynthetic sensitivity to DNA damage


2900761Antonia
mat2-P...or mat2-Pc/Pi?Silenced (waiting for GeneDB to update cassette info so this can be annotated)IMP mat1-
mat3-M...or mat3-Mc/Mi?Silenced (waiting for GeneDB to update cassette info so this can be annotated)IMP mat1-
mat1-MiPhenotype: can conjugate but not undergo meiosis/sporulateallele=Mifs15(del_nt43-129)
mat1-PiPhenotype: can conjugate but not undergo meiosis/sporulateallele=Piop7(del_nt19-357)
mat1-PcPhenotype: can't conjugateallele=Pcop5(del_nt17-480)
mat1-McPhenotype: can't conjugateallele=Mcop7(del_nt19-546)
mat1-Pcsevere reduction in spore formationallele=deletion
mat1-Pcprocess: required for efficient meiosisIMP, allele=deletion
mat1-Mcprocess: required for efficient meiosisIMP, allele=deletion
mat1-Pcexpression: constitutively expressed (low levels) (rich media)IMP, allele=SP272(h+/h- meiotically competent diploid)
mat1-Pcexpression: constitutively expressed (low levels) (rich media)IMP, allele=SP720(fus1 deletion...capture as WT?)
mat1-Mcexpression: constitutively expressed (low levels) (rich media)IMP, allele=SP272(h+/h- meiotically competent diploid)
mat1-Mcexpression: constitutively expressed (low levels) (rich media)IMP, allele=SP720(fus1 deletion...capture as WT?)
mat1-Piexpression: not expressed (rich media)IMP, allele=SP272(h+/h- meiotically competent diploid)
mat1-Piexpression: not expressed (rich media)IMP, allele=SP720(fus1 deletion...capture as WT?)
mat1-Miexpression: not expressed (rich media)IMP, allele=SP272(h+/h- meiotically competent diploid)
mat1-Miexpression: not expressed (rich media)IMP, allele=SP720(fus1 deletion...capture as WT?)
All 4 are upregulated in response to nitrogen starvation. In diploid; downregulation at 6h compared to 4h, in haploid; no downregulation


2328719Antonia
map1transcription phenotype, target is not upregulated in response to nitrogen starvationallele=deletion,annotation_extension=has_regulation_target(GeneDB_Spombe:mat1-PC,(strain is H90))
mat1-Pcpositive autoregulation of transcription in response to nitrogen starvationallele=mat1-Pc-161()
map1transcription phenotype, target is not expressed in response to nitrogen starvationallele=deletion,annotation_extension=has_regulation_target(GeneDB_Spombe:mat1-Pm,(strain is H90))
mat1-Pctranscription phenotype, target is not expressed in response to nitrogen starvationallele=mat1-Pc-161(),annotation_extension=has_regulation_target(GeneDB_Spombe:mat1-Pm,(strain is H90))
mat1-Pclow constitutive expression in rich mediaallele=H90(fus1 deletion)
mat1-Pmno constitutive expression in rich mediaallele=H90(fus1 deletion)
mat1-Pmexpression in response to nitrogen starvation is dependent on the presence of CL:0002674annotation_extension=occurs_in(CL:0002675)
mat1-Pcupregulation in response to nitrogen starvation is not dependent on the presence of CL:0002674annotation_extension=occurs_in(CL:0002675)
ran1process: negative regulation of transcription from RNA polymerase II promoter during mitosis (IMP evidence code)allele=pat1-114,annotation_extension=has_regulation_target(GeneDB_Spombe:mat1-Pm, mat1-Mc and Mat1-Pc


17452352
sequence feature annotate AACCCT as a GATA transcription factor binding site, seq 5'-ATCA(C/A)AACCCTAACCCT-3'do we annotate this on the genome sequence?


18077559|Antonia
ams2 hip1slow cell growthallele=dbl mutant


20230746Antonia
ams2 remove NAS and TAS from artemis
ams2protein is unstable during G2 and M phase, partially stable during G1 and stable during S
ams2protein stability: is stable during S phase, level increased
ams2protein stability: is unstable during G2 phase, level decreased OR possibly only synthesised during S phase?
ams2protein stability: is unstable during G1/M phase, level increased OR possibly only synthesised during S phase?
ams2protein stability: is degraded by the SCF ubiquitin ligase pathway


18158900Antonia
double mutantsim3 mutants (allele=143(G81E),allele=205(E207K)) + overexpressed cnp1 = normal phenotype
double mutantsim3 mutants (allele=143(G81E),allele=205(E207K)) + overexpressed H4 = normal phenotype
double mutantsim3 mutants (allele=143(G81E),allele=205(E207K)) + overexpressed H3 = reduced viability (inviable ok)


cnb1,ppb1dbl deletion mutant same phenotype as each single mutant (branched,elongated multi-septate cells + slow growth in presence of mgcl2worth annotating? i.e. shows may be linked perhaps? dbl mutant not more severe
cnb1,ppb1,pmp1dbl del + overexpressed = normal cell growth
cnb1,ppb1deletion,overexpr. slow growth in presence of mgcl2i.e. large amounts of catalytic domain is not functional without regulatory subunit. is this interesting to capture?
cnb1,ppb1∆C(L445->STOPdeletion,overexpressed. slow growth in presence of mgcl2constitutively active mutant is not active without regulatory subunit
cnb1,ppb1∆C(L445->STOPoverexpressed,overexpressed. phenotype growth arrest, round small bent pear-shaped cells. depolarized distr of cortical F-actin patchesnormal phenotype in presence of CHEBI:61049
cnb1,ppb1∆C(L445->STOP,prz1overexpressed,overexpressed, deleted. no growth arrest


20018864Antonia
deletion(prz1), overexpression(ncs1)slow growthcell growth assay
deletion(ncs1), overexpression(prz1)slow growthcell growth assay
deletion(prz1), overexpression(ncs1)inviable in_response_to(CHEBI:29108)IMP
deletion(ncs1), overexpression(prz1)inviable in_response_to(CHEBI:29108)IMP
deletion(ncs1),deletion(yam8)normal cell growth in_presence_of(CHEBI:33120cell growth assay
ncs1promoter region lies in -1-130 region caact esp important
ncs1promoter for upregulation in response to calcium lies in -101-130 region
ncs1promoter for basal expression lies in -1-130 region
prz1binds caact in promoter region of ncs1


19606215Antonia
cnx1dbl mutant phenotype: abnormal cell wall morphologyallele=lumenal_cnx1p(aa_del488-560),allele=C-termTM_Cnx1p_cmyc(aa_del1-415)
cnx1some confusing data regarding apoptosis. Notes in hardcopy paperallele=lumenal_cnx1p(aa_del488-560)
cnx1allele specifications may not be 100% correct


18621924Antonia
cyr1,cgs1 dbl deletionnuclear export abolishedallele=deletion,annotation_extension=localizes(GeneDBSpombe:SPBC106.10)


map3/mam2diploid strain (CL:0000415) cannot sporulate if both receptors expressed at the same time


1563351Antonia
MatPi?expressed in response to nitrogen starvation and M factor in PcellsIDA
ste6MatPi? not expressed during N starvation and in presence of M factor in P cellsallele=deletion
ras1MatPi? not expressed during N starvation and in presence of M factor in P cellsallele=deletion
ras1MatPi? expressed during N starvation and in presence of M factor in P cells, not constitutively expressedallele=ras1val17
pat1/ras1mat1-Pm transcribedallele=pat1-114/deletion, at high temp
pat1/ste6mat1-Pm transcribedallele=pat1-114/deletion, at high temp

[BR]

8196631Antonia
mfm1/mfm2FYPO:0000584 decreased sporulationallele=deletion (both)
mfm1/mfm3FYPO:0000584 decreased sporulationallele=deletion (both)
mfm2/mfm3FYPO:0000584 decreased sporulationallele=deletion (both)
mfm1/mfm2/mfm3FYPO:0000583 sporulation abolishedallele=deletion (all 3)
mfm1/mfm2/mfm3FYPO:0000583 sporulation abolishedallele=deletion (all 3),annotation_extension=in_presence_of(M-factor - externally added),annotation_extension=occurs_in:CL0002674
mfm1/mfm2/mfm3FYPO:0000590 normal sporulationallele=deletion (all 3),annotation_extension=occurs_in:CL0002674


7975894Antonia
byr1decreased protein level during nitrogen starvationallele=sterile-byr1, assayed_using(PomBase:SPMTR.02), reporter gene assay
ste2decreased protein level during nitrogen starvationallele=sterile-ste2, assayed_using(PomBase:SPMTR.02), reporter gene assay
ste4decreased protein level during nitrogen starvationallele=sterile-ste4, assayed_using(PomBase:SPMTR.02), reporter gene assay
ras1decreased protein level during nitrogen starvationallele=sterile-ras1, assayed_using(PomBase:SPMTR.02), reporter gene assay
ste6decreased protein level during nitrogen starvationallele=sterile-ste6, assayed_using(PomBase:SPMTR.02), reporter gene assay
ste7decreased protein level during nitrogen starvationallele=sterile-ste7, assayed_using(PomBase:SPMTR.02), reporter gene assay
byr2decreased protein level during nitrogen starvationallele=sterile-byr2, assayed_using(PomBase:SPMTR.02), reporter gene assay
ste9decreased protein level during nitrogen starvationallele=sterile-ste9, assayed_using(PomBase:SPMTR.02), reporter gene assay
gpa1decreased protein level during nitrogen starvationallele=sterile-gpa1, assayed_using(PomBase:SPMTR.02), reporter gene assay
spk1decreased protein level during nitrogen starvationallele=sterile-spk1, assayed_using(PomBase:SPMTR.02), reporter gene assay


8314086Antonia
sxa2expressed in presence of p-factor
map2expressed in H- cells
cyr1/sxa2phenotype G1 arrest in presence of P-factor in rich mediaallele=deletion
cyr1/sxa2abnormal/increased shmoo formation in presence of P-factor in rich mediaallele=deletion


18059475Antonia
fkh2/fhl1reduced zygote formationallele=deletion
fkh2/mei4reduced zygote formationallele=deletion
fkh2/fhl1/mei4reduced zygote formationallele=deletion
ste11annotate promoter proximal element FLEX1 and FLEXL1 to ste11, artemis
cig2/fkh2increased zygote formationallele=deletion,happens_during(GO:0006995 - cellular response to nitrogen starvation)
cig2/fkh2/fhl1reduced zygote formationallele=deletion,happens_during(GO:0006995 - cellular response to nitrogen starvation)
cig2/fkh2/mei4reduced zygote formationallele=deletion,happens_during(GO:0006995 - cellular response to nitrogen starvation)
cig2/fkh2/fhl1/mei4reduced zygote formationallele=deletion,happens_during(GO:0006995 - cellular response to nitrogen starvation)


21118960
srw1 ectopically expressed | reb1 deletionnormal cell cycle arrest in response to pheromonephenotype
reb1|wee1phenotype: slow growth at high temperatureallele=deletion(reb1)/wee1-50
reb1|wee1phenotype: multiseptate (0000118)allele=deletion(reb1)/wee1-50
reb1|wee1phenotype: weeallele=deletion(reb1)/wee1-50
reb1|wee1phenotype: spheroid cellsallele=deletion(reb1)/wee1-50
reb1|wee1phenotype: DNA content increasedallele=deletion(reb1)/wee1-50
reb1|wee1phenotype: normal cell growth at high temperaturein_presence_of:CHEBI:44423, allele=deletion(reb1)/wee1-50
reb1|cdc10phenotype: slow growth at high temperatureallele=deletion(reb1)/cdc10-129
reb1|cdc10phenotype: abnormal? cell cycle arrestqualifier=at_high_temperature,allele=deletion(reb1)/cdc10-129


9427748
deletion ppb1|overexpression pmp1suppresses salt stress sensitivity
deletion pmp1|expression of const. active ppb1deltaC (C-term truncated)suppresses salt stress sensitivity
deletion pmp1|expression of const. active ppb1deltaC (C-term truncated)suppresses FK506 sensitivity
deletion pmp1|expression of ppb1 overexpressedsensitive to salt stress and FK506
deletion pmp1|deletion ppb1|deletion pmk1normal cell growth
deletion ppb1|deletionpmk1filamentous and multiseptateallele=deletion
deletion ppb1|overexpression pmp1normal morphology


11461899
stm1|ras1absence of G1 arrest in response to nitrogen starvationallele+deletion (both) flow cytometry
stm1|ras1slow cell growth in minimal media containing ammonia nitrogen sourceallele=deletion (both) cell growth assay
stm1|gpa2normal cell growth in minimal media containing ammonia nitrogen sourceannotation_extension=condition(overexpression - stm1)allele=deletion (gpa2) cell growth assay
stm1|gpa2normal septationannotation_extension=condition(overexpression - stm1)allele=deletion (gpa2) cell growth assay


21960007
mag1structure PDB ID 3S61according to authors, does not appear to match however


1448087
pyp2552 bp 5' UTR
pyp1|pyp2inviable germinating sporeallele=deletion (both)
pyp1 cdc25cell cycle arrestannotation_extension=condition(overexpression, pyp1), allele=cdc25-22, anotation_extension=condition(at_normal_temperature)
pyp2 cdc25cell cycle arrestannotation_extension=condition(overexpression, pyp1), allele=cdc25-22, anotation_extension=condition(at_normal_temperature)
pyp1 cdc25cell cycle arrestannotation_extension=condition(overexpression, pyp1), allele=cdc25-22, anotation_extension=condition(at_high_temperature)
pyp2 cdc25cell cycle arrestannotation_extension=condition(overexpression, pyp1), allele=cdc25-22, anotation_extension=condition(at_high_temperature)
pyp1 cdc2normal cell length (cell division/cell cycle)annotation_extension=condition(overexpression, pyp1), allele=cdc2-1w
pyp2 cdc2normal cell length (cell division/cell cycle)annotation_extension=condition(overexpression, pyp1), allele=cdc2-1w
pyp1 wee1elongated cellsannotation_extension=condition(overexpression, pyp1), allele=wee1-50, anotation_extension=condition(at_normal_temperature)
pyp2 wee1elongated cellsannotation_extension=condition(overexpression, pyp1), allele=wee1-50, anotation_extension=condition(at_normal_temperature)
pyp1 wee1normal cell length (possibly could use normal cell growth or normal cell division/mitotic cell cycleannotation_extension=condition(overexpression, pyp1), allele=wee1-50, anotation_extension=condition(at_high_temperature)
pyp2 wee1normal cell length (possibly could use normal cell growth or normal cell division/mitotic cell cycleannotation_extension=condition(overexpression, pyp1), allele=wee1-50, anotation_extension=condition(at_high_temperature)
pyp1 cdc25normal cell lengthallele=deletion, pyp1), allele=cdc25-22, annotation_extension=condition(at_normal_temperature)
pyp2 cdc25elongated cellsallele=deletion, pyp1), allele=cdc25-22, annotation_extension=condition(at_normal_temperature)
pyp1 cdc25elongated cellsallele=deletion, pyp1), allele=cdc25-22, annotation_extension=condition(at_high_temperature)


11470243
fas2increased accumulation of see belowallele=H201(I1276T),annotation_extension=condition(at_high_temperature)
fas2increased accumulation of see belowallele=H265(Q4Y),annotation_extension=condition(at_high_temperature)
fas2increased accumulation of see belowallele=H518(I600N),annotation_extension=condition(at_high_temperature)
fas2decreased accumulation of see belowallele=H201(I1276T),annotation_extension=condition(at_high_temperature)
fas2decreased accumulation of see belowallele=H265(Q4Y),annotation_extension=condition(at_high_temperature)
fas2decreased accumulation of see belowallele=H518(I600N),annotation_extension=condition(at_high_temperature)

For the 3 mutants above:
increased accumulation of at high temp: N-monomethyldioleoyl-PE (N-monomethyl-C18:1-C18:1-PE) N,N-dimethyl-C18:1-C18:1-PE N,N-monomethyl-C18:1-C18:1-PE N,N-dimethyl-C16:1-C18:1-PE 1-melissoyl-oleolyl-sn-glycero-3-phosphoethanolamine (C30:0-C18:1-PE) 1-melissoyl-2-oleolyl-sn-glycero-3-phosphocholine (C30:0-C18:1-PC) CHEBI:16337 CHEBI:36711


decreased accumulation of at high temp: (NOT in mutant 518) 1-oleolyl-2-caproyl-sn-glycero-3-phosphocholine (C18:1-C10:0-PC) C18:0-C10:0-PC C18:0-C8:0-PC CHEBI:18303 CHEBI:17517


15075260
pdf1dbl mutant normal growth in presence of sodium orthovanadateallele=S106A(S106A),allele=H478A(H478A) (2 plasmids)
pdf1dbl mutant normal growth in presence of sodium orthovanadateallele=D226A(D226A),allele=H478A(H478A) (2 plasmids)
pdf1dbl mutant normal growth in presence of 'near alkaline/high' pH (=condition?)allele=S106A(S106A),allele=H478A(H478A) (2 plasmids)
pdf1dbl mutant normal growth in presence of 'near alkaline/high' pH (=condition?)allele=D226A(D226A),allele=H478A(H478A) (2 plasmids)


9017391http://curation.pombase.org/pombe/curs/40aa30b33b608f68
gcs1 + ade6sensitive to cadmiumallele=apd1-1(unknown),allele=ade6-210(unknown)
gcs1 + ade6sensitive to cadmiumallele=deletion,allele=ade6-210(unknown))
gcs1 + ade6normal growth in presence of either ions: Ca|Na|Mgallele=apd1-1(unknown),allele=ade6-210(unknown)
gcs1 + ade6normal cell growthannotation_extension=condition(in_rich_media),allele=apd1-1(unknown),allele=ade6-210(unknown)
gcs1 + ade6resistant to MNNG (N-methyl-N'-nitro-N-nitrosoguanidine)allele=deletion,allele=ade6-210(unknown))|
gcs1 + ade6slow cell growthannotation_extension=condition(in_minimal_media),allele=apd1-1(unknown),allele=ade6-210(unknown)
gcs1 + ade6slow cell growthannotation_extension=condition(in_minimal_media),allele=deletion,allele=ade6-210(unknown)
gsh2 + ade6slow cell growthannotation_extension=condition(in_minimal_media),allele=deletion,allele=ade6-210(unknown)
hmt1 + ade6normal cell growthannotation_extension=condition(in_minimal_media),allele=deletion,allele=ade6-210(unknown)
gcs1 + ade6normal cell growthannotation_extension=condition(in_minimal_media),annotation_extension=in_presence_of(CHEBI:16856),allele=apd1-1(unknown),allele=ade6-210(unknown)
gcs1 + ade6normal cell growthannotation_extension=condition(in_minimal_media),annotation_extension=in_presence_of(CHEBI:16856),allele=deletion,allele=ade6-210(unknown)
gsh2 + ade6normal cell growthannotation_extension=condition(in_minimal_media),annotation_extension=in_presence_of(CHEBI:16856),allele=deletion,allele=ade6-210(unknown)
gcs1 + ade6sporulation abolishedallele=apd1-1(unknown),allele=ade6-210(unknown)
gcs1 + ade6normal sporulationannotation_extension=in_presence_of(CHEBI:16856),allele=apd1-1(unknown),allele=ade6-210(unknown)
hmt1 + ade6red pigmentation of coloniesallele=deletion,allele=ade6-210(unknown)
gcs1 + ade6absent/reduced red pigmentation of coloniesallele=apd1-1(unknown),allele=ade6-210(unknown)
gcs1 + ade6absent/reduced red pigmentation of coloniesallele=deletion,allele=ade6-210(unknown)
gsh2 + ade6reduced red pigmentation of coloniesallele=deletion,allele=ade6-210(unknown)
gcs1 + ade6absent/reduced red pigmentation of coloniesannotation_extension=condition(in_minimal_media),annotation_extension=in_presence_of(CHEBI:16856),allele=apd1-1(unknown),allele=ade6-210(unknown)


1448066
ade2/ade6dbl mutant sensitive to cadmiumallele=ade6-216(unknown),allele=ade2(deletion), growth assay
ade2/ade7dbl mutant sensitive to cadmiumallele=ade7-50(unknown),allele=ade2(deletion), growth assay
-asked for a term to describe HMW complex. Link to it
ade2/ade6dbl mutant can't accumulate HMW PC-Cd-S2- complexallele=ade6-216(unknown),allele=ade2(deletion)
ade2/ade7dbl mutant can't accumulate HMW PC-Cd-S2- complexallele=ade7-50(unknown),allele=ade2(deletion)
-asked for a term to describe HMW complex. Link to it - GO:0090424


12905027
SPAC3H1.10MOD requested: cadmium containing modified residueresidue=C173|


9016341
ade2 + ade7sensitive to cisplatinallele=deletion (ade2) allele=ade7.50
ade2 + ade6sensitive to cisplatinallele=deletion (ade2) allele=ade6.216


16928959
perhaps should use +ve reg of transcr instead of calcium response for the var agentsgo over again


19646873
gef1delta + orb6-25 normal morphology at high tempmicroscopy
rga4 overexpressed + orb6-25 normal morphology at high tempmicroscopy
Gef1 overexpression + orb6-as2stubby PCO:0000005 at normal temp
orb6-25 + rga4deltastubby at normal temp PCO:0000005


19285552
rgs1decreased transcriptional response in response to pheromoneallele=noname(overexpression),​​annotation_extension=occurs_in(CL:0002674)
gpa1can probably migrate some terms to abnormal decrease in transcription/increase, see what midori says


20396879
dak1/dak2 dbl mutnormal cell growthallele=dak1-delta(deletion),allele=dak2-delta(deletion),condition=PCO:0000014
dak1/dak2decreased glycerol dehydrogenase activityallele=dak1-delta(deletion),allele=dak2-delta(deletion),condition=PCO:0000072
dak1/dak2increased accumulation of DHAallele=dak1-delta(deletion),allele=dak2-delta(deletion),condition=PCO:0000072
dak1/dak2slow aerobic cell growthallele=dak1-delta(deletion),allele=ak2-delta(deletion),condition=PCO:0000072
dak1/dak2/gld1abolished glycerol dehydrogenase activityallele=dak1-delta(deletion),allele=dak2-delta(deletion),allele=gld1-delta(deletion),condition=PCO:0000072
dak1/dak2slow aerobic cell growthallele=dak1-delta(deletion),allele=ak2-delta(deletion),condition=PCO:0000073
dak1/dak2slow aerobic cell growthallele=dak1-delta(deletion),allele=ak2-delta(deletion),condition=PCO:0000076,condition=PCO:0000014
tup11/tup12increased glycerol dehydrogenase activityallele=tup11-delta(deletion),allele=tup12-delta(deletion),condition=PCO:0000014
gld1/dak1/dak2slow cell growthallele=noname(overexpression),condition=PCO:0000077


9585505
atf1 + pap1sensitive to cisplatinallele=atf1-delta(deletion), allele=pap1-delta(deletion), condition=PCO:0000012, CGA


1448080
crm1 + pap1normal growth at cold /or/ normal cell growth condition=low tempallele=crm1-809(unknown),allele=pap1-delta(deletion),PCO:0000012
crm1 + pap1sensitive to staurosporineallele=crm1-809(unknown),allele=pap1-delta(deletion),PCO:0000012
crm1 + pap1normal protein accumulationallele=crm1-809(unknown),allele=pap1-delta(deletion),annotation_extension=has_regulation_target(PomBase:SPAC3C7.14c)


7642144
rpn11| pmd1resistant to K-252aallele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012
rpn11| pmd1resistant to staurosporineallele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012
rpn11| pmd1resistant to vanadateallele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012
rpn11| pmd1resistant to cycloheximideallele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012
rpn11| pmd1resistant to actinomycin Dqualifier=NOT, allele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012
rpn11| pmd1resistant to thiabendazolequalifier=NOT, allele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012


21324894
cia1 | mts2increased protein level allele=asf1-30(F24S,​G120S,​D173G), allele=mts2-1(unknown), condition=PCO:0000004,annotation_extension=has_regulation/downstream_target(PomBase:SPCC663.05c), western
cia1 | mts2slow growth allele=asf1-30(F24S,​G120S,​D173G), allele=mts2-1(unknown), condition=PCO:0000005, condition=PCO:0000012, CGA
cia1 | mts2slow growth at high temp allele=asf1-30(F24S,​G120S,​D173G), allele=mts2-1(unknown), condition=PCO:0000012, CGA
cia1 | ubc4slow growth allele=asf1-30(F24S,​G120S,​D173G), allele=ubc4ts(P61S), condition=PCO:0000005, condition=PCO:0000012, CGA
cia1 | ubc4slow growth at high temp allele=asf1-30(F24S,​G120S,​D173G), allele=ubc4ts(P61S), condition=PCO:0000012, CGA
cia1 + mts2FYPO:0000783, cia1 normally found in nucleus, but here found in cytoplasmallele=asf1-30(F24S,​G120S,​D173G),allele=mts2-1(unknown), condition=PCO:0000004, specify cia in annex? has_target cia1?
cia1 | ubc4increased protein level allele=asf1-30(F24S,​G120S,​D173G), allele=ubc4ts(P61S), condition=PCO:0000004,annotation_extension=has_regulation/downstream_target(PomBase:SPCC663.05c), western
cia1 | SPBC2A(.04Cincreased protein level allele=asf1-30(F24S,​G120S,​D173G), allele=san1-delta(deletion), condition=PCO:0000004,annotation_extension=has_regulation/downstream_target(PomBase:SPCC663.05c), western
cia1 | hrd1 slow growth at normal temp allele=asf1-30(F24S,​G120S,​D173G),allele=hrd1-delta(deletion), condition=PCO:0000012
cia1 | hrd1 slow growth at high temp allele=asf1-30(F24S,​G120S,​D173G),allele=hrd1-delta(deletion), condition=PCO:0000012
cia1 | doa10 slow growth at normal temp allele=asf1-30(F24S,​G120S,​D173G),allele=doa10-delta(deletion), condition=PCO:0000012
cia1 | doa10 slow growth at high temp allele=asf1-30(F24S,​G120S,​D173G),allele=doa10-delta(deletion), condition=PCO:0000012
cia1 | san1 normal cell growth allele=asf1-30(F24S,​G120S,​D173G),allele=san1-delta(deletion), condition=PCO:0000012, condition=PCO:0000006
cia1 | san1 slow growth at high temp allele=asf1-30(F24S,​G120S,​D173G),allele=san1-delta(deletion), condition=PCO:0000012
cia1 | ubr1 normal cell growth allele=asf1-30(F24S,​G120S,​D173G),allele=ubr1-delta(deletion), condition=PCO:0000012, condition=PCO:0000006
cia1 | ubr1 slow growth at high temp allele=asf1-30(F24S,​G120S,​D173G),allele=ubr1-delta(deletion), condition=PCO:0000012
cia1 | ubr11 normal cell growth allele=asf1-30(F24S,​G120S,​D173G),allele=ubr11-delta(deletion), condition=PCO:0000012, condition=PCO:0000006
cia1 | ubr11 slow growth at high temp allele=asf1-30(F24S,​G120S,​D173G),allele=ubr11-delta(deletion), condition=PCO:0000012
SPBC2A9.04c | mis12 normal cell growth at high tempallele=san1-delta(deletion),allele=mis12-537(unknown), condition=PCO:0000012, condition=PCO:0000012
SPBC2A9.04c | pim1 normal cell growth at high tempallele=san1-delta(deletion),allele=pim1-46(unknown), condition=PCO:0000012, condition=PCO:0000012
SPBC2A9.04c | cnp1 normal cell growth at high tempallele=san1-delta(deletion),allele=cnp1-1(unknown), condition=PCO:0000012, condition=PCO:0000012


9191271
mfm1dbl check annotations, allele descr for phenotype annotationhas_regulation_target(PomBase:SPMTR.02))


8321236http://oliver0.sysbiol.cam.ac.uk/pombe/curs/169dc6a662075c56/gene/1
frp1abolished ferric iron reductase activity, not req yetallele=G-100(W314->opal)
waiting reply from val on paper


7565608http://oliver0.sysbiol.cam.ac.uk/pombe/curs/07cc7abfbaa6d1b0
rds1 /controlled_curation="term=gene expression, mRNA level; annotation_extension=during(cellular response to adenine starvation); qualifier=increased; evidence=ECO:0000106; db_xref=7565608; date=20120504"


9135147http://oliver0.sysbiol.cam.ac.uk/pombe/curs/67ba83cbaba024a7
pmk1 | spk1sterileallele=pmk1delta(deletion), allele=spk1delta(deletion)
pmk1 | spk1abnormal morphologyallele=pmk1delta(deletion), allele=spk1delta(deletion), condition=PCO:0000014, condition=PCO:0000102
pmk1 | spk1normal response to salt stressallele=pmk1delta(deletion), allele=spk1delta(deletion), condition=PCO:0000014, condition=PCO:0000102
wis1 | pmk1 multiseptate microscopy, allele=wi1delta(deletion), allele=pmk1delta(deletion), condition=PCO:0000014, condition=PCO:0000102


1644306http://oliver0.sysbiol.cam.ac.uk/pombe/curs/1b8ed217ce984ab5
tnr3/thi1change FYPO terms so that extension is in term
tnr1increased RNA level, northern evidence, need to find what this gene is...allele=tnr1-18(unknown), annotation_extension=assayed_using(SPBC26H8.01),​ condition=PCO:0000014,​ annotation_extension=happens_during(GO:0071301)
tnr1cellular response to vitamin b1 - which gene is this?? IMP
thi2gene expression: expressed in response to thiamine starvation. repressed in response to thiamine....
/controlled_curation="term=gene expression, mRNA level; annotation_extension=during(GO:0036225); qualifier=increased; evidence=ECO:0000106; db_xref=1644306; date=20120509"
/controlled_curation="term=gene expression, mRNA level; annotation_extension=during(GO:0071301); qualifier=absent; evidence=ECO:0000106; db_xref=1644306; date=20120509"
/controlled_curation="term=gene expression, mRNA level; annotation_extension=in_response_to(CHEBI:16892); qualifier=increased; evidence=ECO:0000106; db_xref=1644306; date=20120509"
/controlled_curation="term=gene expression, mRNA level; annotation_extension=in_response_to(CHEBI:17957); qualifier=decreased; evidence=ECO:0000106; db_xref=1644306; date=20120509"


8675019http://oliver0.sysbiol.cam.ac.uk/pombe/curs/4b0970d0e5484486/
tnr1which gene is this? FYPO:0000825 northern evallele=tnr1-18(unknown), condition=PCO:0000014, annotation_extension=assayed_using(SPAC17A2.01)
thi3 | bsu1 slow aerobic cell growth allele=bsu1delta(deletion), allele=thi3-1(unknown), condition=PCO:0000014, condition=PCO:0000106
thi3 | bsu1 slow aerobic cell growth allele=bsu1delta(deletion), allele=thi3-1(unknown), condition=PCO:0000014, condition=PCO:0000107


2249257http://oliver0.sysbiol.cam.ac.uk/pombe/curs/40213c281e540075

/controlled_curation="term=gene expression, mRNA level; annotation_extension=during(cellular response to thiamine starvation); qualifier=increased; evidence=ECO:0000106; db_xref=2249257; date=20120510"


9560432http://oliver0.sysbiol.cam.ac.uk/pombe/curs/de924eb687e556b7
itr2 | itr1 normal cell growth allele=IM49(unknown),​​ allele=itr1-noname(overexpression), condition=PCO:0000005,​​ condition=PCO:0000014,​​ condition=PCO:0000102,​ condition=PCO:0000111


22245228http://curation.pombase.org/pombe/curs/7b18cacb1717e352
tpx1 | srx1 normal cellular response to hydrogen peroxideallele=srx1(overexpression), allele=tpx1(deletion)


10455235http://oliver0.sysbiol.cam.ac.uk/pombe/curs/32450f2b6ffd928f
gpx1 | ctt1 normal response to hydrogen peroxide ctt1=deleted, gpx1=overexpressed, 30 degrees
transcription factor deletionsreuest terms for during h202 and normal growthsee paper annotations


8549830http://curation.pombase.org/pombe/curs/78253143f2002b71
hxk1/hxk2slow growth on glucose carbon sourcedouble-deletion


2878925http://curation.pombase.org/pombe/curs/462720c354cd7b67/
pma mutantnormal ATPase activity unknown, in vitro
pma mutantNormal enzyme in vitro response to Dio-9 unknown, in vitro
pma mutantNormal enzyme in vitro response to DCCD N,N'-dicyclohexylcarbodiimideunknown, in vitro
pma mutantNormal enzyme in vitro response to Miconazoleunknown, in vitro
pma mutantNormal enzyme in vitro response to DES diethylstilbestrolunknown, in vitro
pma mutantNormal enzyme in vitro response to Suloctidil unknown, in vitro
pma mutantNormal enzyme in vitro response to p-hydroxymercuribenzoate unknown, in vitro
pma mutantNormal enzyme in vitro response to protamine sulfate unknown, in vitro
pma mutantNormal enzyme in vitro response to decamethylene diguanidineunknown, in vitro
ade7-413|pma mutant slow growth adenine added, MM, liquid media
pma mutant decreased methionine transport into cell during nitrogen starvation
pma mutant decreased cellular pH CGAYE liquid


11136464http://curation.pombase.org/pombe/curs/0089b9465b71c321/
inv1|sut1slow growth on sucroseallele=deletion(both), sucrose MM
inv1|sut1slow growth on trehaloseallele=deletion(both), trehalose MM
inv1|sut1normal growth on maltoseallele=deletion(both), maltose MM


17384198http://curation.pombase.org/pombe/curs/42b53662d27d7951
other assaysRNA protection assay or transcript quantification assay


9813085http://curation.pombase.org/pombe/curs/b91556d0478e3dea
alg6|gpt1decreased glc1man9GlcNAc levelDNJ added, chromatography, deletion of both
alg6|gpt1increased man9GlcNAc levelDNJ addedchromatography, deletion of both
alg6|gpt1absent glc2man9GlcNAcDNJ added, chromatography, deletion of both
alg6|gpt1absent glc3man9GlcNAcDNJ added, chromatography, deletion of both
alg6|gpt1decreased glc1man9GlcNAc levelchromatography, deletion of both
alg6|gpt1decreased man9GlcNAc levelchromatography, deletion of both
alg6|gpt1absent glc2man9GlcNAcchromatography, deletion of both
alg6|gpt1absent glc3man9GlcNAcchromatography, deletion of both
gls2|alg6absent glc2man9GlcNAcchromatography, deletion of both
gls2|alg6absent glc3man9GlcNAcchromatography, deletion of both
gls2|alg6increased glc1man9GlcNAcchromatography, deletion of both
gls2|alg6increased man9GlcNAcchromatography, deletion of both
gls2|alg6increased man8GlcNAcchromatography, deletion of both
gpt1|alg6increased RNA levelnorthern, annotation_extension=assayed_using(PomBase:SPAC22A12.15c)
gpt1|alg6increased RNA levelnorthern, annotation_extension=assayed_using(PomBase:SPAC22A12.15c)
alg6lower cell density in stationary phasedeletion, glucose minimal medium, liquid, normal temp
gpt1 | alg6lower cell density in stationary phasedeletion, glucose minimal medium, liquid, normal temp
gpt1 | alg6slower cell growth ratedeletion, glucose minimal medium, liquid, normal temp
gpt1 | alg6flocculating cells, microscopydeletion, glucose minimal medium, normal temp
gpt1 | alg6round/swollen cells (they are not small), microscopydeletion, glucose minimal medium, normal temp
gpt1 | alg6slow growth at high temperaturedeletion, glucose minimal medium, normal temp
gls2 | alg6slow aerobic cell growth on glucose carbon sourcenormal glucose MM, high temp, agar plates
gls2 | alg6normal aerobic cell growth on glucose carbon sourcenormal glucose MM, normal temp
gpt1 | alg6slow aerobic cell growth on glucose carbon sourcenormal glucose MM, normal temp, agar plates
gpt1 | alg6slow aerobic cell growth on glucose carbon sourcenormal glucose MM, high temp, agar plates
deletion alg6 | overexpressed gpt1normal cell growthplate, normal glucose MM, normal temp
deletion alg6 | overexpressed gpt1normal cell growthplate, normal glucose MM, high temp
gpt1|alg6normal morphologydeletion, sorbitol added, normal glucose MM, liquid
gpt1|alg6normal cell growth on gluocose carbon sourcedeletion, sorbitol added, normal glucose MM
deletion alg6 | overexpressed gpt1normal morphologynormal glucose MM, high temp
deletion alg6| deletion gpt1|overexpression gpt1(Y1312A,D1352A)round/swollen cellsnormal temp, glucose MM
deletion alg6| deletion gpt1|overexpression gpt1(Y1312A,D1352A)slow growth at high temphigh temp, glucose MM
gls2 | alg6normal morphologynormal glucose MM, normal temp


11016847http://curation.pombase.org/pombe/curs/373f4c6a1e37adf9
yam8 | pck2normal growth at high tempallele=ehs1-1, allele=pck2(overexpressed),high temp, YES
yam8 | pck2slow growth at high tempallele=yam8delta, allele=pck2(overexpressed),high temp, YES
yam8 | pck2normal conjugation freqallele=ehs1-1, allele=pck2(overexpressed)
yam8 | pck2decreased conjugation freqallele=yam8delta, allele=pck2(overexpressed)
yam8 | pck2normal growth on cilofunginallele=ehs1-1, allele=pck2(overexpressed),normal temp, YES
yam8 | pck2sensitive to cilofunginallele=yam8delta, allele=pck2(overexpressed),normal temp, YES
yam8 | pck2normal growth on calcofluor whiteallele=ehs1-1, allele=pck2(overexpressed),normal temp, YES
yam8 | pck2sensitive to calcofluor whiteallele=yam8delta, allele=pck2(overexpressed),normal temp, YES
yam8 | pck2slow growthallele=pck2(overexpression),allele=ehs1-1, solid agar plates, glucose minimal medium, standard temperature
yam8 | pck2normal cell growthallele=pck2(overexpression),allele=ehs1-1, calcium excluded, solid agar plates, glucose minimal medium, standard temperature
yam8 | pck2 normal growthallele=pck2(overexpression),allele=yam8delta, solid agar plates, glucose minimal medium, standard temperature
yam8 | pck2 normal growthallele=pck2(overexpression),allele=yam8delta, calcium excluded, solid agar plates, glucose minimal medium, standard temperature
yam8 | pck2normal level of calcium in cellallele=ehs1-1, allele=pck2(overexpression), normal temp, glucose MM
yam8 | pck2inviableallele=ehs1-1, allele=pck2delta
yam8 | pck2viableallele=yam8delta, allele=pck2delta
yam8 | pck2normal morphologyallele=pck2(overexpression),allele=yam8delta, glucose MM, standard temp microscopy
yam8 | pck2lysed cellsallele=pck2(overexpression),allele=ehs1-1, glucose MM, standard temp microscopy
yam8 | pck1sensitive to calcofluor whiteallele=ehs1-1, allele=pck1(overexpressed),normal temp, YES
yam8 | pck1sensitive to cilofunginallele=ehs1-1, allele=pck1(overexpressed),normal temp, YES
yam8 | pck1slow growth at high tempallele=ehs1-1, allele=pck1(overexpressed),high temp, YES


7898433http://curation.pombase.org/pombe/curs/5964257cca6cfa69
uvi15gene expression, change in response to L-canavanine to during the above once the term is inartemis


12896976
pap1 | hba1 sensitive to caffeine pap1 deleted, hba1 overexpressed, standard glucose MM, standard temp, agar plates
pap1 | hba1 sensitive to caffeine pap1 deleted, hba1 deleted, standard glucose rich medium, standard temp, agar plates
sty1 | hba1 resistant to caffeine sty1 deleted, hba1 deleted, standard glucose MM (yeah,diff from above), standard temp, agar plates


22017871http://curation.pombase.org/pombe/curs/4d5512a5dc8ea4bc

Phenotypes:

ubr1increased protein level during cellular response to hypoxiaallele=deletion,assayed_using(sre1 precursor)
ubr1increased protein level during cellular response to hypoxiaallele=deletion,assayed_using(PR:000028992)
ubr1increased protein level during cellular response to oxygenallele=deletion,assayed_using(PR:000028992)
rhp6increased protein level during cellular response to hypoxiaallele=deletion,assayed_using(PR:000028992)
rhp6normal protein level during cellular response to hypoxiaallele=deletion,assayed_using(sre1 precursor)
rhp6increased protein level during cellular response to oxygenallele=deletion,assayed_using(PR:000028992)
sre1 | ubr11 normal protein level during cellular response to hypoxiaallele=sre1-N(recheck, cellpress down atm), allele=ubr11-delta,assayed_using(PR:000028992)
sre1 | ubr11 normal protein level during cellular response to oxygenallele=sre1-N(recheck, cellpress down atm), allele=ubr11-delta,assayed_using(PR:000028992)
sre1 normal protein level during cellular response to hypoxiaallele=sre1-N(recheck, cellpress down atm),assayed_using(PR:000028992)
sre1 | ofd1increased protein level during cellular response to oxygenallele=ofd1delta, allele=sre1-N, assayed_using(PR:000028992)
sre1 | ofd1increased protein level during cellular response to hypoxiaallele=ofd1delta, allele=sre1-N, assayed_using(PR:000028992)
ubr1 | ofd1increased protein level during cellular response to oxygenallele=ofd1delta, allele=ubr1delta, assayed_using(PR:000028992)
ubr1 | ofd1increased protein level during cellular response to hypoxiaallele=ofd1delta, allele=ubr1delta, assayed_using(PR:000028992)
ubr1 | nro1increased protein level during cellular response to oxygenallele=nro1delta, allele=ubr1delta, assayed_using(PR:000028992)
ubr1 | nro1increased protein level during cellular response to hypoxiaallele=nro1delta, allele=ubr1delta, assayed_using(PR:000028992)
ofd1increased protein level during cellular response to oxygenallele=ofd1delta, assayed_using(PomBase:SPAC222.11), NORTHERN
nro1decreased RNA level during cellular response to hypoxiaallele=deleteion, assayed using(PomBase:SPAC222.11), northern
nro1normal RNA level during cellular response to hypoxiaallele=deleteion, assayed using(PomBase:SPBC19C2.09), northern
ofd1decreased protein binding during cellular response to hypoxiaallele=H142A, assayed_using(PomBase:SPCC4B3.07)
ofd1overexpressed ofd1 C terminal domain -> decreased binding of sre1 to hem13 (chip assay)

Processes:

general:have requested cellular response to oxygen use TG after I get this
ofd1negative regulation of sterol regulatory element DNA bindingannotation_extension=requires_sequence_feature(Pfam:13640) | annotation_extension=happens_during(cellular response to oxygen
nro1+ve reg of cellular response to hypoxiaIDA


17905925http://curation.pombase.org/pombe/curs/61b0bcb9898f64f7
pps1normal cell growthallele=deletion, standard temperature, agar plates, standard glucose rich medium, ethanolamine added
pps1-GFPdecreased growthallele=c-term gfp fusion, yes, normal temp, plates
pps1-GFPnormal growthallele=c-term gfp fusion, yes, ethanolamine added, normal temp, plates
GFP-pps1normal growthn-term gfp fusion, MM, plates, normal temp


756560807cc7abfbaa6d1b0
rds1delta | ade6-D1normal phenotype


18071249http://curation.pombase.org/pombe/curs/5bff0cacfd0d068a
lcf1 | lcf2decreased myristoyl-ligase activitydouble deletion
lcf1 | lcf2abolished palmitoyl-ligase activitydouble deletion
lcf1 | lcf2abolished oleoyl-ligase activitydouble deletion
lcf1 | lcf2decreased viability in stationary phasedouble deletion, standard glucose MM
lcf1 | lcf2decreased viability in stationary phasedouble deletion, growth to stationary phase in rich medium followed by transfer to H2O
lcf1 | lcf2decreased viability in stationary phasedouble deletion, growth to stationary phase in rich medium followed by transfer to H2O
lcf1 | lcf2decreased cell density in stationary phaseallele= double deletion, growth to stationary phase in rich medium followed by transfer to minimal medium


21811607http://curation.pombase.org/pombe/curs/db6b03f9221f5e4a/
cam1normal immediate intracellular calcium spike following extracellular calcium stimulusenzyme assay, allele=cam1-1(unknown)
ncs1normal immidiate intracellular calcium spike following extracellular calcium stimulusenzyme assay, allele=deletion
spcc633.14c(trp663)normal intracellular calcium spike following extracellular calcium stimulusenzyme assay, overexpression
spcc1322.03(trp1322)increased intracellular calcium spike following extracellular calcium stimulusenzyme assay, overexpression
1322.03increased transcription from CDRE promoter during vegetative growth, increased basal transcription from CDRE (SO:0001865) promoter/increased transcription from CRDE promoter in the absence of calciumreporter gene assay, overexpression
1322.03absent down regulation/attenuation of transcription from CDRE promoter in response to calciumreporter gene assay, overexpression, calcium added
1322.03C-terminal GFP tag renders protein non-functionalenzyme assay?
pkd2positive regulation (need to TG) of Golgi calcium ion exportIMP
pmc1golgi calcium ion exportIMP
pmc1GO:0051209 if it is extended to include golgiIMP
pkd2GO:0051209 if is extended to include the golgiIMP
pkd2 | pmr1normal immediate intracellular calcium spike following extracellular calcium stimulusenzyme assay,allele=overexpression pkd2 / deletion(pmr1)
pkd2 | pmc1normal immediate intracellular calcium spike following extracellular calcium stimulusenzyme assay,allele=overexpression pkd2 / deletion(pmc1)
pkd2 | vcx1normal immediate intracellular calcium spike following extracellular calcium stimulusenzyme assay,allele=overexpression pkd2 / deletion(vcx1)
pkd2increased prolonged cellular calcium level overexpression, enzyme assay
trp1322normal prolonged cellular calcium level overexpression, enzyme assay
trp1322 | pmr1normal prolonged cellular calcium level 1322overexpression, pmr1delta, enzyme assay
pkd2 | pmr1normal prolonged cellular calcium level pkd2overexpression, pmr1delta, enzyme assay
yam8increased cellular calcium level in response to sodium ionsdeletion, enzyme assay
cch1increased cellular calcium level in response to sodium ionsdeletion, enzyme assay
yam8 | cch1increased cellular calcium level in response to sodium ionsdoubledeletion, enzyme assay
ppb1increased cellular calcium level in response to sodium ionsdeletion, enzyme assay
yam8resistant to fk506deletion, Na ions added, enzyme assay data
cch1resistant to fk506deletion, Na ions added, enzyme assay data
ppb1resistant to fk506deletion, Na ions added, enzyme assay data
pmk1decreased cellular calcium leveldeletion, enzyme assay, sodium ions and FK506 added
mkh1decreased cellular calcium leveldeletion, enzyme assay, sodium ions and FK506 added
pek1decreased cellular calcium leveldeletion, enzyme assay, sodium ions and FK506 added
ppb1normal cellular response to fk506deletion, cga, plates, normal temp
pmk1normal cellular response to fk506deletion, cga, plates, normal temp
trp1322normal cellular response to fk506deletion, cga, plates, normal temp
ppb1normal cellular response to fk506 and sorbitoldeletion, cga, plates, normal temp
pmk1normal cellular response to fk506 and sorbitoldeletion, cga, plates, normal temp
trp1322normal cellular response to fk506 and sorbitoldeletion, cga, plates, normal temp
ppb1normal cellular response to fk506 and salt stressdeletion, cga, plates, normal temp
pmk1resistant to fk506 and salt stressdeletion, cga, plates, normal temp
trp1322resistant to fk506 and salt stressdeletion, cga, plates, normal temp
pek1increased calcium level in response to salt stresspek1DD(unknown), overexpression, enzyme assay
pek1 | cch1normal calcium level in response to salt stresspek1DD(unknown), overexpression, cch1delta, enzyme assay
pek1 | yam8normal calcium level in response to salt stresspek1DD(unknown), overexpression, yam8delta, enzyme assay
pek1 | yam8normal calcium level in response to salt stresspek1DD(unknown), overexpression, yam8delta, FK506 added, enzyme assay
pmk1normal nuclear morphologymicroscopy, deletin, FK506 and Mg ions added
pkd2increased cellular calcium level during salt stressoverexpression
1322normal cellular calcium level during salt stressoverexpression
1322decreased cellular calcium level during salt stress tacrolimus added
pkd2normal cellular calcium level during salt stress
1322increased transcription from CRDE promoter (SO:0001865) in the absence of calcium / increased basal transcription from CDREpromoter overexpression
1322GO:1901198 Label: positive regulation of calcium ion transport into cytosol involved in cellular response to calcium ionIMP
1322GO:1901200 Label: negative regulation of calcium ion transport into cytosol involved in cellular response to salt stressIMP
1322increased transcription from CDRE promoter during vegetative growth/increased transcription from CDRE promoter (SO:0001865) in the absence of salt stress / increased basal transcription from CDREpromoter overexpression, reporter gene assay
1322decreased celluilar calcium level during salt stressoverexpression, reporter gene assay
1322 | ppb1normal cellular response to salt stressoverexpression of 1322 and deletion of ppb1, cell growth assay, Na ions OR magnesium ions added
1322|prz1decreased immediate intracellular calcium spike following extracellular calcium stimulus 1322 overexpressed, prz1delta
1322|ppb1absent immediate intracellular calcium spike following extracellular calcium stimulus 1322 overexpressed, ppb1delta
pkd2|prz1decreased immediate intracellular calcium spike following extracellular calcium stimulus pkd2 overexpressed, prz1delta
pkd2|prz1decreased immediate intracellular calcium spike following extracellular calcium stimulus pkd2 overexpressed, ppb1delta
1322decreased calcium level during salt stress1322delta
1322decreased calcium level during salt stress1322delta, tacrolimus added
1322|pek1normal cellular calcium level during salt stress1322-delta, pek1DD(unknown)
1322|pek1normal cellular calcium level during salt stress1322-delta, pek1DD(unknown), tacrolimus added


9573052
pom1|cdc25monopolar actin cortical patch localization allele=pom1delta, allele=cdc25-22, rhodamine-phalloidin added, high temp
pom1|cdc10monopolar actin cortical patch localization to either end allele=pom1delta, allele=cdc10-V50, rhodamine-phalloidin added, high temp
pom1|cdc25activation of monopolar cell growth at either endallele=pom1delta, allele=cdc25-22, rhodamine-phalloidin added, high temp
pom1|cdc10activation of monopolar cell growth at either endallele=pom1delta, allele=cdc10-V50, rhodamine-phalloidin added, high temp
pom1|cdc11branched cellspom1delta,cdc11-136, high temp, calcofluor added
pom1|act1slow growthpom1delta, act1-48, at high, low and standard temp
pom1|cdc3slow growthpom1delta, cdc3-6, low temp, standard temp
pom1|cdc8slow growthpom1delta, cdc8-110, low temp, standard temp
pom1|nda3slow growthpom1delta, nda3-KM311, standard temp
pom1|ban5slow growthpom1delta, ban5-3, high temp, standard temp
pom1|cdc14slow growthpom1delta, cdc14-118
pom1|mid1slow growthpom1delta, dmf1-6
pom1|tea1viablepom1delta, tea1delta, high temp, standard temp, low temp
pom1|tea1branched cellspom1delta, tea1delta, high temp, standard temp, low temp
pom1|tea1mislocalized septumpom1delta, tea1delta


10224084http://curation.pombase.org/pombe/curs/169c0f8873eee853
hmt2sensitive to sulfide JS563, sodium sulfide added
hmt2sensitive to sulfide deletion, sodium sulfide added
hmt2|sir1normal cellular acid labile sulfide (S2-) levelhmt2JS563(E396K)|sir1delta, rich medium
2832071http://curation.pombase.org/pombe/curs/d5c757529d95fb29
pma1sensitive to protamine sulfateat high temp, CGA, pma(unknown)


10223994http://curation.pombase.org/pombe/curs/b0116284c3e493a0
tps1|ntp1resistant to freeze-thawingallele=tps1(overexpressed),ntp1(deletion), EMM, solid agar, growth to exponential phase
tps1|ntp1resistant to dehydrationallele=tps1(overexpressed),ntp1(deletion), EMM, solid agar, growth to exponential phase
tps1|ntp1resistant to ethanolallele=tps1(overexpressed),ntp1(deletion), EMM, solid agar, growth to exponential phase
tps1|ntp1increased trehalose level during veg growthallele=tps1(overexpressed),ntp1(deletion), MM, growth to exponential phase
tps1|ntp1increased trehalose level in response to heat shockallele=tps1(overexpressed),ntp1(deletion), MM, heat shock 30 mins 48 degrees, growth to exponential phase
tps1|ntp1resistance to heat shockCGA, allele=tps1(overexpressed),ntp1(deletion), MM, heat shock 30 mins 48 degrees, liquid culture, growth to exponential phase


14612233http://curation.pombase.org/pombe/curs/e73b6293989d19a9
gda1decreased guanosine-diphosphatase activityallele=deletion
gda1increased guanosine-diphosphatase activityallele=overexpression
gda1adenosine triphosphatase activity, change ATP termqualifier=NOT, IDA


9729425http://curation.pombase.org/pombe/curs/17c0501f20dc6e13/
tps1absent increase in trehalase activity following nutrient stimulusdeletion, enzyme assay, rich glycerol medium, exponential phase, glucose and asparagine added
tps1absent increase in trehalase activity following nutrient stimulusdeletion, enzyme assay, low glucose rich medium, stationary phase, glucose and asparagine added
tps1absent increase in trehalase activity in response to high temperaturedeletion, enzyme assay, high temp,
tps1normal increase in trehalase activity in response to salt stressdeletion, enzyme assay, NaCl? added
tps1normal rna level in response to heatdeletion, annotation_extension=assayed_using(PomBase:SPBC660.07)
tps1normal rna level in response to salt stressdeletion, NaCl? added, annotation_extension=assayed_using(PomBase:SPBC660.07)


9532803http://curation.pombase.org/pombe/curs/a826f97af4e46f8f
pap1|rpn11sensitive to brefeldin Apap1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|rpn11normal response to staurosporinepap1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|rpn11sensitive to K-252apap1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|rpn11sensitive to actinomycin Dpap1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|rpn11sensitive to cytochalasin Bpap1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|bfr1resistant to brefeldin Apap1delta, bfr1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|bfr1resistant to staurosporinepap1delta, bfr1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|bfr1resistant to K-252apap1delta, bfr1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|bfr1resistant to actinomycin Dpap1delta, bfr1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|bfr1resistant to cytochalasin Bpap1delta, bfr1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|rpn11sensitive to brefeldin Abfr1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|rpn11resistant to staurosporinebfr1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|rpn11sensitive to K-252abfr1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|rpn11sensitive to actinomycin Dbfr1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|rpn11sensitive to cytochalasin Bbfr1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|pap1sensitive to brefeldin Abfr1delta, pap1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|pap1resistant to staurosporinebfr1delta, pap1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|pap1sensitive to K-252abfr1delta, pap1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|pap1sensitive to actinomycin Dbfr1delta, pap1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|pap1normal cellular response to cytochalasin Bbfr1delta, pap1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added


10867006ste11 promoter


|?|
mat1-pmdecreased trehalase activity during sporulationmei1-B102(unknown),sporulation medium
mat1-pmdecreased trehalose level during sporulationmei1-B102(unknown),sporulation medium
mat1-pmnormal trehalase activity during veg growthmei1-B102(unknown),YES medium


15920625http://curation.pombase.org/pombe/curs/75dadd0ae174dd58/
ggt2increased gamma-glutamyl transpeptidase activityallele=overexpression
ggt2increased cellular GSH levelallele=overexpression
ggt2resistant to mercuryallele=overexpression, MM, agar
ggt2resistant to aluminium/aluminumallele=overexpression, MM, agar
ggt2resistant to diethylmaleateallele=overexpression, MM, agar


11318103http://curation.pombase.org/pombe/curs/f6bbb1302b15a4e8/
varin curs NTRs
vardextrose YE condition


|16262791
|http://curation.pombase.org/pombe/curs/722def8c5161e3f1/
varNTRs in session
rhb1normal GTP bindingS21G, enzyme assay data
rhb1normal GTP bindingK120R, enzyme assay data
rhb1normal GTP bindingN153T, enzyme assay data
rhb1decreased GTP bindingV17G, enzyme assay data
rhb1decreased GDP bindingV17G, enzyme assay data
rhb1decreased GDP bindingS21G, enzyme assay data
rhb1decreased GDP bindingK120R, enzyme assay data
rhb1decreased GDP bindingN153T, enzyme assay data
rhb1decreased GTPase activityS21G, enzyme assay data
rhb1decreased GTPase activityV17G, enzyme assay data
rhb1normal GTPase activityN153T, enzyme assay data
rhb1normal GTPase activityK120R, enzyme assay data
rhb1increased protein bindingK120R, annotation_extension=assayed_using(PomBase:SPBC216.07c), copurification and cosedimentation( western blot)
rhb1increased protein bindingV17G, annotation_extension=assayed_using(PomBase:SPBC216.07c), co-sedimentation(western blot)
rhb1increased protein bindingN153T, annotation_extension=assayed_using(PomBase:SPBC216.07c), co-sedimentation(western blot)
tsc1increased protein bindingN153T, annotation_extension=assayed_using(PomBase:SPBC428.16c|SPBC216.07c), co-sedimentation(western blot)
tsc2|rhb1normal cellular response to canavanineallele=tsc2delta,rhb1(D60K), CGA, agar plates, emm


18637840http://curation.pombase.org/pombe/curs/b719dc2cd003f460
zrt1high affinity zinc ion import, NTR, replace 2 bpsIMP
zrt1sensitive to zinc starvationdeletion, liquid culture, standard temp,
zrt1sensitive to zinc starvationdeletion, agar plates, standard temp, EMM
zrt1 | zhf1 sensitive to zinc and zinc starvation both deleted, liquid, standard temp
zrt1 | zhf1 sensitive to zinc and zinc starvation both deleted, agar, standard temp, emm
zrt1reduced cellular zinc levelliquid scintillation counter/AAS/substance quantification, deletion, EMM, zinc added
zhf1reduced cellular zinc levelliquid scintillation counter/AAS/substance quantification, deletion, EMM, zinc added
zrt1 | zhf1reduced cellular zinc levelliquid scintillation counter/AAS/substance quantification, both deleted, EMM, zinc added
zip2normal cellular zinc levelspectroscopy, EMM, zinc added, deletion
zip3normal cellular zinc levelspectroscopy, EMM, zinc added, deletion
zip2normal cellular copper levelspectroscopy, EMM, zinc added, deletion
zip3normal cellular copper levelspectroscopy, EMM, zinc added, deletion
zrt1normal cellular copper levelspectroscopy, EMM, zinc added, deletion
zhf1normal cellular copper levelspectroscopy, EMM, zinc added, deletion
zrt1 | zhf1normal cellular copper levelspectroscopy, EMM, zinc added, both deleted
zip2normal cellular iron levelspectroscopy, EMM, zinc added, deletion
zip3normal cellular iron levelspectroscopy, EMM, zinc added, deletion
zrt1normal cellular iron levelspectroscopy, EMM, zinc added, deletion
zhf1normal cellular iron levelspectroscopy, EMM, zinc added, deletion
zrt1 | zhf1normal cellular iron levelspectroscopy, EMM, zinc added, both deleted
zip2normal cellular potassium levelspectroscopy, EMM, zinc added, deletion
zip3normal cellular potassium levelspectroscopy, EMM, zinc added, deletion
zrt1normal cellular potassium levelspectroscopy, EMM, zinc added, deletion
zhf1normal cellular potassium levelspectroscopy, EMM, zinc added, deletion
zrt1 | zhf1normal cellular potassium levelspectroscopy, EMM, zinc added, both deleted
zip2normal cellular magnesium levelspectroscopy, EMM, zinc added, deletion
zip3normal cellular magnesium levelspectroscopy, EMM, zinc added, deletion
zrt1normal cellular magnesium levelspectroscopy, EMM, zinc added, deletion
zhf1normal cellular magnesium levelspectroscopy, EMM, zinc added, deletion
zrt1 | zhf1normal cellular magnesium levelspectroscopy, EMM, zinc added, both deleted
zip2normal cellular manganese levelspectroscopy, EMM, zinc added, deletion
zip3normal cellular manganese levelspectroscopy, EMM, zinc added, deletion
zrt1normal cellular manganese levelspectroscopy, EMM, zinc added, deletion
zhf1normal cellular manganese levelspectroscopy, EMM, zinc added, deletion
zrt1 | zhf1normal cellular manganese levelspectroscopy, EMM, zinc added, both deleted
zip2normal cellular molybdenum levelspectroscopy, zinc added, EMM, deletion
zip3normal cellular molybdenum levelspectroscopy, EMM, zinc added, deletion
zrt1normal cellular molybdenum levelspectroscopy, EMM, zinc added, deletion
zhf1normal cellular molybdenum levelspectroscopy, EMM, zinc added, deletion
zrt1 | zhf1normal cellular molybdenum levelspectroscopy, EMM, zinc added, both deleted
zip2normal cellular nickel levelspectroscopy, EMM, zinc added, deletion
zip3normal cellular nickel levelspectroscopy, EMM, zinc added, deletion
zrt1normal cellular nickel levelspectroscopy, EMM, zinc added, deletion
zhf1normal cellular nickel levelspectroscopy, EMM, zinc added, deletion
zrt1 | zhf1normal cellular nickel levelspectroscopy, EMM, zinc added, both deleted
zip2normal cellular phosphorus levelspectroscopy, EMM, zinc added, deletion
zip3normal cellular phosphorus levelspectroscopy, EMM, zinc added, deletion
zrt1normal cellular phosphorus levelspectroscopy, EMM, zinc added, deletion
zhf1normal cellular phosphorus levelspectroscopy, EMM, zinc added, deletion
zrt1 | zhf1normal cellular phosphorus levelspectroscopy, EMM, zinc added, both deleted
zip2normal cellular sulfur levelspectroscopy, EMM, zinc added, deletion
zip3normal cellular sulfur levelspectroscopy, EMM, zinc added, deletion
zrt1normal cellular sulfur levelspectroscopy, EMM, zinc added, deletion
zhf1normal cellular sulfur levelspectroscopy, EMM, zinc added, deletion
zrt1 | zhf1normal cellular sulfur levelspectroscopy, EMM, zinc added, both deleted
zhf1decreased RNA level during cellular response to zinc iondeletion, annotation_extension=assayed_using(PomBase:SPBC16D10.06) RT-PCR
zhf1increased RNA level during cellular response to zinc ion starvationdeletion, annotation_extension=assayed_using(PomBase:SPBC16D10.06) RT-PCR
zhf1increased protein level during cellular response to zinc starvationdeletion, annotation_extension=assayed_using(PomBase:SPBC16D10.06) western
zhf1abnormal protein level during cellular response to zincdeletion, annotation_extension=assayed_using(PomBase:SPBC16D10.06) western

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