AntoniasCuratedPapers

AntoniasDoubleMutants2

21745468	Antonia
lkh1, dsk1	((phenotype)) slow cell growth (cell growth assay)	allele=Δdsk1Δlkh1
lkh1, dsk1	((phenotype)) slow cell growth (cell growth assay)	allele=Δdsk1,lkh1-K391A

7900424
mfm1/2/3	triple deletion, sporulation abolished	diploid
mfm1/2/3	triple deletion, normal sporulation	diploid, M-factor added
mfm1/2/3	triple deletion, normal sporulation	diploid, P-factor added
mfm1/2/3	triple deletion, decreased protein level	has regulation target(Pfactor), diploid

17369611	Antonia
rtt109	Delta rtt109/Histone H3 K56R double-mutant phenotype	asynthetic sensitivity to DNA damage

18158900	Antonia
double mutant	sim3 mutants (allele=143(G81E),allele=205(E207K)) + overexpressed cnp1 = normal phenotype
double mutant	sim3 mutants (allele=143(G81E),allele=205(E207K)) + overexpressed H4 = normal phenotype
double mutant	sim3 mutants (allele=143(G81E),allele=205(E207K)) + overexpressed H3 = reduced viability (inviable ok)

cnb1,ppb1	dbl deletion mutant same phenotype as each single mutant (branched,elongated multi-septate cells + slow growth in presence of mgcl2	worth annotating? i.e. shows may be linked perhaps? dbl mutant not more severe
cnb1,ppb1,pmp1	dbl del + overexpressed = normal cell growth
cnb1,ppb1	deletion,overexpr. slow growth in presence of mgcl2	i.e. large amounts of catalytic domain is not functional without regulatory subunit. is this interesting to capture?
cnb1,ppb1∆C(L445->STOP	deletion,overexpressed. slow growth in presence of mgcl2	constitutively active mutant is not active without regulatory subunit
cnb1,ppb1∆C(L445->STOP	overexpressed,overexpressed. phenotype growth arrest, round small bent pear-shaped cells. depolarized distr of cortical F-actin patches	normal phenotype in presence of CHEBI:61049
cnb1,ppb1∆C(L445->STOP,prz1	overexpressed,overexpressed, deleted. no growth arrest

20018864	Antonia
deletion(prz1), overexpression(ncs1)	slow growth	cell growth assay
deletion(ncs1), overexpression(prz1)	slow growth	cell growth assay
deletion(prz1), overexpression(ncs1)	inviable in_response_to(CHEBI:29108)	IMP
deletion(ncs1), overexpression(prz1)	inviable in_response_to(CHEBI:29108)	IMP
deletion(ncs1),deletion(yam8)	normal cell growth in_presence_of(CHEBI:33120	cell growth assay

19606215	Antonia
cnx1	dbl mutant phenotype: abnormal cell wall morphology	allele=lumenal_cnx1p(aa_del488-560),allele=C-termTM_Cnx1p_cmyc(aa_del1-415)
check alleles

18621924	Antonia
cyr1,cgs1 dbl deletion	nuclear export abolished	allele=deletion,annotation_extension=localizes(GeneDBSpombe:SPBC106.10)

map3/mam2

diploid strain (CL:0000415) cannot sporulate if both receptors expressed at the same time

1563351	Antonia
pat1/ras1	mat1-Pm transcribed	allele=pat1-114/deletion, at high temp
pat1/ste6	mat1-Pm transcribed	allele=pat1-114/deletion, at high temp

[BR]

8196631	Antonia
mfm1/mfm2	FYPO:0000584 decreased sporulation	allele=deletion (both)
mfm1/mfm3	FYPO:0000584 decreased sporulation	allele=deletion (both)
mfm2/mfm3	FYPO:0000584 decreased sporulation	allele=deletion (both)
mfm1/mfm2/mfm3	FYPO:0000583 sporulation abolished	allele=deletion (all 3)
mfm1/mfm2/mfm3	FYPO:0000583 sporulation abolished	allele=deletion (all 3),annotation_extension=in_presence_of(M-factor - externally added),annotation_extension=occurs_in:CL0002674
mfm1/mfm2/mfm3	FYPO:0000590 normal sporulation	allele=deletion (all 3),annotation_extension=occurs_in:CL0002674

8314086	Antonia
cyr1/sxa2	phenotype G1 arrest in presence of P-factor in rich media	allele=deletion
cyr1/sxa2	abnormal/increased shmoo formation in presence of P-factor in rich media	allele=deletion

18059475	Antonia
fkh2/fhl1	reduced zygote formation	allele=deletion
fkh2/mei4	reduced zygote formation	allele=deletion
fkh2/fhl1/mei4	reduced zygote formation	allele=deletion
cig2/fkh2	increased zygote formation	allele=deletion,happens_during(GO:0006995 - cellular response to nitrogen starvation)
cig2/fkh2/fhl1	reduced zygote formation	allele=deletion,happens_during(GO:0006995 - cellular response to nitrogen starvation)
cig2/fkh2/mei4	reduced zygote formation	allele=deletion,happens_during(GO:0006995 - cellular response to nitrogen starvation)
cig2/fkh2/fhl1/mei4	reduced zygote formation	allele=deletion,happens_during(GO:0006995 - cellular response to nitrogen starvation)

21118960
srw1 ectopically expressed | reb1 deletion	normal cell cycle arrest in response to pheromone	phenotype
reb1|wee1	phenotype: inviable high temp	allele=deletion(reb1)/wee1-50, cga, high temp
reb1|wee1	phenotype: multiseptate, multinucleate (mitosis before cytokinesis)	allele=deletion(reb1)/wee1-50
reb1|wee1	phenotype: wee and spheroid/round	allele=deletion(reb1)/wee1-50
reb1|wee1	phenotype: DNA content increased (more than 2c)	allele=deletion(reb1)/wee1-50
reb1|wee1	phenotype: normal cell growth at high temperature	in_presence_of:CHEBI:44423, allele=deletion(reb1)/wee1-50
reb1|cdc10	phenotype: inviable high temperature	allele=deletion(reb1)/cdc10-129, yes
reb1|cdc10	decreased cell pop growth std temp	allele=deletion(reb1)/cdc10-129, yes
reb1|cdc10	phenotype: abnormal? cell cycle arrest	qualifier=at_high_temperature,allele=deletion(reb1)/cdc10-129

9427748
deletion ppb1|overexpression pmp1	suppresses salt stress sensitivity
deletion pmp1|expression of const. active ppb1deltaC (C-term truncated)	suppresses salt stress sensitivity
deletion pmp1|expression of const. active ppb1deltaC (C-term truncated)	suppresses FK506 sensitivity
deletion pmp1|expression of ppb1 overexpressed	sensitive to salt stress and FK506
deletion pmp1|deletion ppb1|deletion pmk1	normal cell growth
deletion ppb1|deletionpmk1	filamentous and multiseptate	allele=deletion
deletion ppb1|overexpression pmp1	normal morphology

11461899
stm1|ras1	absence of G1 arrest in response to nitrogen starvation	allele+deletion (both) flow cytometry
stm1|ras1	slow cell growth in minimal media containing ammonia nitrogen source	allele=deletion (both) cell growth assay
stm1|gpa2	normal cell growth in minimal media containing ammonia nitrogen source	annotation_extension=condition(overexpression - stm1)allele=deletion (gpa2) cell growth assay
stm1|gpa2	normal septation	annotation_extension=condition(overexpression - stm1)allele=deletion (gpa2) cell growth assay

1448087
pyp1|pyp2	inviable germinating spore	allele=deletion (both)
pyp1 cdc25	cell cycle arrest	annotation_extension=condition(overexpression, pyp1), allele=cdc25-22, anotation_extension=condition(at_normal_temperature)
pyp2 cdc25	cell cycle arrest	annotation_extension=condition(overexpression, pyp1), allele=cdc25-22, anotation_extension=condition(at_normal_temperature)
pyp1 cdc25	cell cycle arrest	annotation_extension=condition(overexpression, pyp1), allele=cdc25-22, anotation_extension=condition(at_high_temperature)
pyp2 cdc25	cell cycle arrest	annotation_extension=condition(overexpression, pyp1), allele=cdc25-22, anotation_extension=condition(at_high_temperature)

pyp1 cdc2	normal cell length (cell division/cell cycle)	annotation_extension=condition(overexpression, pyp1), allele=cdc2-1w
pyp2 cdc2	normal cell length (cell division/cell cycle)	annotation_extension=condition(overexpression, pyp1), allele=cdc2-1w
pyp1 wee1	elongated cells	annotation_extension=condition(overexpression, pyp1), allele=wee1-50, anotation_extension=condition(at_normal_temperature)
pyp2 wee1	elongated cells	annotation_extension=condition(overexpression, pyp1), allele=wee1-50, anotation_extension=condition(at_normal_temperature)
pyp1 wee1	normal cell length (possibly could use normal cell growth or normal cell division/mitotic cell cycle	annotation_extension=condition(overexpression, pyp1), allele=wee1-50, anotation_extension=condition(at_high_temperature)
pyp2 wee1	normal cell length (possibly could use normal cell growth or normal cell division/mitotic cell cycle	annotation_extension=condition(overexpression, pyp1), allele=wee1-50, anotation_extension=condition(at_high_temperature)

pyp1 cdc25	normal cell length	allele=deletion, pyp1), allele=cdc25-22, annotation_extension=condition(at_normal_temperature)
pyp2 cdc25	elongated cells	allele=deletion, pyp1), allele=cdc25-22, annotation_extension=condition(at_normal_temperature)
pyp1 cdc25	elongated cells	allele=deletion, pyp1), allele=cdc25-22, annotation_extension=condition(at_high_temperature)

15075260
pdf1	dbl mutant normal growth in presence of sodium orthovanadate	allele=S106A(S106A),allele=H478A(H478A) (2 plasmids)
pdf1	dbl mutant normal growth in presence of sodium orthovanadate	allele=D226A(D226A),allele=H478A(H478A) (2 plasmids)
pdf1	dbl mutant normal growth in presence of 'near alkaline/high' pH (=condition?)	allele=S106A(S106A),allele=H478A(H478A) (2 plasmids)
pdf1	dbl mutant normal growth in presence of 'near alkaline/high' pH (=condition?)	allele=D226A(D226A),allele=H478A(H478A) (2 plasmids)

9017391	​http://curation.pombase.org/pombe/curs/40aa30b33b608f68
gcs1 + ade6	sensitive to cadmium	allele=apd1-1(unknown),allele=ade6-210(unknown)
gcs1 + ade6	sensitive to cadmium	allele=deletion,allele=ade6-210(unknown))
gcs1 + ade6	normal growth in presence of either ions: Ca|Na|Mg	allele=apd1-1(unknown),allele=ade6-210(unknown)
gcs1 + ade6	normal cell growth	annotation_extension=condition(in_rich_media),allele=apd1-1(unknown),allele=ade6-210(unknown)
gcs1 + ade6	resistant to MNNG (N-methyl-N'-nitro-N-nitrosoguanidine)	allele=deletion,allele=ade6-210(unknown))|
gcs1 + ade6	slow cell growth	annotation_extension=condition(in_minimal_media),allele=apd1-1(unknown),allele=ade6-210(unknown)
gcs1 + ade6	slow cell growth	annotation_extension=condition(in_minimal_media),allele=deletion,allele=ade6-210(unknown)
gsh2 + ade6	slow cell growth	annotation_extension=condition(in_minimal_media),allele=deletion,allele=ade6-210(unknown)
hmt1 + ade6	normal cell growth	annotation_extension=condition(in_minimal_media),allele=deletion,allele=ade6-210(unknown)
gcs1 + ade6	normal cell growth	annotation_extension=condition(in_minimal_media),annotation_extension=in_presence_of(CHEBI:16856),allele=apd1-1(unknown),allele=ade6-210(unknown)
gcs1 + ade6	normal cell growth	annotation_extension=condition(in_minimal_media),annotation_extension=in_presence_of(CHEBI:16856),allele=deletion,allele=ade6-210(unknown)
gsh2 + ade6	normal cell growth	annotation_extension=condition(in_minimal_media),annotation_extension=in_presence_of(CHEBI:16856),allele=deletion,allele=ade6-210(unknown)
gcs1 + ade6	sporulation abolished	allele=apd1-1(unknown),allele=ade6-210(unknown)
gcs1 + ade6	normal sporulation	annotation_extension=in_presence_of(CHEBI:16856),allele=apd1-1(unknown),allele=ade6-210(unknown)
hmt1 + ade6	red pigmentation of colonies	allele=deletion,allele=ade6-210(unknown)
gcs1 + ade6	absent/reduced red pigmentation of colonies	allele=apd1-1(unknown),allele=ade6-210(unknown)
gcs1 + ade6	absent/reduced red pigmentation of colonies	allele=deletion,allele=ade6-210(unknown)
gsh2 + ade6	reduced red pigmentation of colonies	allele=deletion,allele=ade6-210(unknown)
gcs1 + ade6	absent/reduced red pigmentation of colonies	annotation_extension=condition(in_minimal_media),annotation_extension=in_presence_of(CHEBI:16856),allele=apd1-1(unknown),allele=ade6-210(unknown)

1448066
ade2/ade6	dbl mutant sensitive to cadmium	allele=ade6-216(unknown),allele=ade2(deletion), growth assay
ade2/ade7	dbl mutant sensitive to cadmium	allele=ade7-50(unknown),allele=ade2(deletion), growth assay
ade2/ade6	dbl mutant can't accumulate HMW PC-Cd-S2- complex	allele=ade6-216(unknown),allele=ade2(deletion)
ade2/ade7	dbl mutant can't accumulate HMW PC-Cd-S2- complex	allele=ade7-50(unknown),allele=ade2(deletion)

9016341
ade2 + ade7	sensitive to cisplatin	allele=deletion (ade2) allele=ade7.50
ade2 + ade6	sensitive to cisplatin	allele=deletion (ade2) allele=ade6.216

19646873
gef1delta + orb6-25	normal morphology at high temp	microscopy
rga4 overexpressed + orb6-25	normal morphology at high temp	microscopy
Gef1 overexpression + orb6-as2	stubby PCO:0000005 at normal temp
orb6-25 + rga4delta	stubby at normal temp PCO:0000005

20396879
dak1/dak2 dbl mut	normal cell growth	allele=dak1-delta(deletion),allele=dak2-delta(deletion),condition=PCO:0000014
dak1/dak2	decreased glycerol dehydrogenase activity	allele=dak1-delta(deletion),allele=dak2-delta(deletion),condition=PCO:0000072
dak1/dak2	increased accumulation of DHA	allele=dak1-delta(deletion),allele=dak2-delta(deletion),condition=PCO:0000072
dak1/dak2	slow aerobic cell growth	allele=dak1-delta(deletion),allele=ak2-delta(deletion),condition=PCO:0000072
dak1/dak2/gld1	abolished glycerol dehydrogenase activity	allele=dak1-delta(deletion),allele=dak2-delta(deletion),allele=gld1-delta(deletion),condition=PCO:0000072
dak1/dak2	slow aerobic cell growth	allele=dak1-delta(deletion),allele=ak2-delta(deletion),condition=PCO:0000073
dak1/dak2	slow aerobic cell growth	allele=dak1-delta(deletion),allele=ak2-delta(deletion),condition=PCO:0000076,condition=PCO:0000014
tup11/tup12	increased glycerol dehydrogenase activity	allele=tup11-delta(deletion),allele=tup12-delta(deletion),condition=PCO:0000014
gld1/dak1/dak2	slow cell growth	allele=noname(overexpression),condition=PCO:0000077

9585505
atf1 + pap1	sensitive to cisplatin	allele=atf1-delta(deletion), allele=pap1-delta(deletion), condition=PCO:0000012, CGA

1448080
crm1 + pap1	normal growth at cold /or/ normal cell growth condition=low temp	allele=crm1-809(unknown),allele=pap1-delta(deletion),PCO:0000012
crm1 + pap1	sensitive to staurosporine	allele=crm1-809(unknown),allele=pap1-delta(deletion),PCO:0000012
crm1 + pap1	normal protein accumulation	allele=crm1-809(unknown),allele=pap1-delta(deletion),annotation_extension=has_regulation_target(PomBase:SPAC3C7.14c)

7642144
rpn11| pmd1	resistant to K-252a	allele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012
rpn11| pmd1	resistant to staurosporine	allele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012
rpn11| pmd1	resistant to vanadate	allele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012
rpn11| pmd1	resistant to cycloheximide	allele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012
rpn11| pmd1	resistant to actinomycin D	qualifier=NOT, allele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012
rpn11| pmd1	resistant to thiabendazole	qualifier=NOT, allele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012

21324894
cia1 | mts2	increased protein level	allele=asf1-30(F24S,​G120S,​D173G), allele=mts2-1(unknown), condition=PCO:0000004,annotation_extension=has_regulation/downstream_target(PomBase:SPCC663.05c), western
cia1 | mts2	slow growth	allele=asf1-30(F24S,​G120S,​D173G), allele=mts2-1(unknown), condition=PCO:0000005, condition=PCO:0000012, CGA
cia1 | mts2	slow growth at high temp	allele=asf1-30(F24S,​G120S,​D173G), allele=mts2-1(unknown), condition=PCO:0000012, CGA
cia1 | ubc4	slow growth	allele=asf1-30(F24S,​G120S,​D173G), allele=ubc4ts(P61S), condition=PCO:0000005, condition=PCO:0000012, CGA
cia1 | ubc4	slow growth at high temp	allele=asf1-30(F24S,​G120S,​D173G), allele=ubc4ts(P61S), condition=PCO:0000012, CGA

cia1 + mts2

FYPO:0000783, cia1 normally found in nucleus, but here found in cytoplasm

allele=asf1-30(F24S,​G120S,​D173G),allele=mts2-1(unknown), condition=PCO:0000004, specify cia in annex? has_target cia1?

cia1 | ubc4	increased protein level	allele=asf1-30(F24S,​G120S,​D173G), allele=ubc4ts(P61S), condition=PCO:0000004,annotation_extension=has_regulation/downstream_target(PomBase:SPCC663.05c), western
cia1 | SPBC2A(.04C	increased protein level	allele=asf1-30(F24S,​G120S,​D173G), allele=san1-delta(deletion), condition=PCO:0000004,annotation_extension=has_regulation/downstream_target(PomBase:SPCC663.05c), western

cia1 | hrd1	slow growth at normal temp	allele=asf1-30(F24S,​G120S,​D173G),allele=hrd1-delta(deletion), condition=PCO:0000012
cia1 | hrd1	slow growth at high temp	allele=asf1-30(F24S,​G120S,​D173G),allele=hrd1-delta(deletion), condition=PCO:0000012
cia1 | doa10	slow growth at normal temp	allele=asf1-30(F24S,​G120S,​D173G),allele=doa10-delta(deletion), condition=PCO:0000012
cia1 | doa10	slow growth at high temp	allele=asf1-30(F24S,​G120S,​D173G),allele=doa10-delta(deletion), condition=PCO:0000012

cia1 | san1	normal cell growth	allele=asf1-30(F24S,​G120S,​D173G),allele=san1-delta(deletion), condition=PCO:0000012, condition=PCO:0000006
cia1 | san1	slow growth at high temp	allele=asf1-30(F24S,​G120S,​D173G),allele=san1-delta(deletion), condition=PCO:0000012
cia1 | ubr1	normal cell growth	allele=asf1-30(F24S,​G120S,​D173G),allele=ubr1-delta(deletion), condition=PCO:0000012, condition=PCO:0000006
cia1 | ubr1	slow growth at high temp	allele=asf1-30(F24S,​G120S,​D173G),allele=ubr1-delta(deletion), condition=PCO:0000012
cia1 | ubr11	normal cell growth	allele=asf1-30(F24S,​G120S,​D173G),allele=ubr11-delta(deletion), condition=PCO:0000012, condition=PCO:0000006
cia1 | ubr11	slow growth at high temp	allele=asf1-30(F24S,​G120S,​D173G),allele=ubr11-delta(deletion), condition=PCO:0000012
SPBC2A9.04c | mis12	normal cell growth at high temp	allele=san1-delta(deletion),allele=mis12-537(unknown), condition=PCO:0000012, condition=PCO:0000012
SPBC2A9.04c | pim1	normal cell growth at high temp	allele=san1-delta(deletion),allele=pim1-46(unknown), condition=PCO:0000012, condition=PCO:0000012
SPBC2A9.04c | cnp1	normal cell growth at high temp	allele=san1-delta(deletion),allele=cnp1-1(unknown), condition=PCO:0000012, condition=PCO:0000012

9135147	​http://oliver0.sysbiol.cam.ac.uk/pombe/curs/67ba83cbaba024a7
pmk1 | spk1	sterile	allele=pmk1delta(deletion), allele=spk1delta(deletion)
pmk1 | spk1	abnormal morphology	allele=pmk1delta(deletion), allele=spk1delta(deletion), condition=PCO:0000014, condition=PCO:0000102
pmk1 | spk1	normal response to salt stress	allele=pmk1delta(deletion), allele=spk1delta(deletion), condition=PCO:0000014, condition=PCO:0000102
wis1 | pmk1	multiseptate	microscopy, allele=wi1delta(deletion), allele=pmk1delta(deletion), condition=PCO:0000014, condition=PCO:0000102

8675019	​http://oliver0.sysbiol.cam.ac.uk/pombe/curs/4b0970d0e5484486/
thi3 | bsu1	slow aerobic cell growth	allele=bsu1delta(deletion), allele=thi3-1(unknown), condition=PCO:0000014, condition=PCO:0000106
thi3 | bsu1	slow aerobic cell growth	allele=bsu1delta(deletion), allele=thi3-1(unknown), condition=PCO:0000014, condition=PCO:0000107

9560432	​http://oliver0.sysbiol.cam.ac.uk/pombe/curs/de924eb687e556b7
itr2 | itr1	normal cell growth	allele=IM49(unknown),​​ allele=itr1-noname(overexpression), condition=PCO:0000005,​​ condition=PCO:0000014,​​ condition=PCO:0000102,​ condition=PCO:0000111

22245228	​http://curation.pombase.org/pombe/curs/7b18cacb1717e352
tpx1 | srx1	normal cellular response to hydrogen peroxide	allele=srx1(overexpression), allele=tpx1(deletion)

10455235	​http://oliver0.sysbiol.cam.ac.uk/pombe/curs/32450f2b6ffd928f
gpx1 | ctt1	normal response to hydrogen peroxide	ctt1=deleted, gpx1=overexpressed, 30 degrees
transcription factor deletions	reuest terms for during h202 and normal growth	see paper annotations

8549830	​http://curation.pombase.org/pombe/curs/78253143f2002b71
hxk1/hxk2	slow growth on glucose carbon source	double-deletion

2878925	​http://curation.pombase.org/pombe/curs/462720c354cd7b67/
ade7-413|pma mutant	slow growth	adenine added, MM, liquid media

11136464	​http://curation.pombase.org/pombe/curs/0089b9465b71c321/
inv1|sut1	slow growth on sucrose	allele=deletion(both), sucrose MM
inv1|sut1	slow growth on trehalose	allele=deletion(both), trehalose MM
inv1|sut1	normal growth on maltose	allele=deletion(both), maltose MM

9813085	​http://curation.pombase.org/pombe/curs/b91556d0478e3dea
alg6|gpt1	decreased glc1man9GlcNAc level	DNJ added, chromatography, deletion of both
alg6|gpt1	increased man9GlcNAc level	DNJ addedchromatography, deletion of both
alg6|gpt1	absent glc2man9GlcNAc	DNJ added, chromatography, deletion of both
alg6|gpt1	absent glc3man9GlcNAc	DNJ added, chromatography, deletion of both
alg6|gpt1	decreased glc1man9GlcNAc level	chromatography, deletion of both
alg6|gpt1	decreased man9GlcNAc level	chromatography, deletion of both
alg6|gpt1	absent glc2man9GlcNAc	chromatography, deletion of both
alg6|gpt1	absent glc3man9GlcNAc	chromatography, deletion of both
gls2|alg6	absent glc2man9GlcNAc	chromatography, deletion of both
gls2|alg6	absent glc3man9GlcNAc	chromatography, deletion of both
gls2|alg6	increased glc1man9GlcNAc	chromatography, deletion of both
gls2|alg6	increased man9GlcNAc	chromatography, deletion of both
gls2|alg6	increased man8GlcNAc	chromatography, deletion of both
gpt1|alg6	increased RNA level	northern, annotation_extension=assayed_using(PomBase:SPAC22A12.15c)
gpt1|alg6	increased RNA level	northern, annotation_extension=assayed_using(PomBase:SPAC22A12.15c)
gpt1 | alg6	lower cell density in stationary phase	deletion, glucose minimal medium, liquid, normal temp
gpt1 | alg6	slower cell growth rate	deletion, glucose minimal medium, liquid, normal temp
gpt1 | alg6	flocculating cells, microscopy	deletion, glucose minimal medium, normal temp
gpt1 | alg6	round/swollen cells (they are not small), microscopy	deletion, glucose minimal medium, normal temp
gpt1 | alg6	slow growth at high temperature	deletion, glucose minimal medium, normal temp
gls2 | alg6	slow aerobic cell growth on glucose carbon source	normal glucose MM, high temp, agar plates
gls2 | alg6	normal aerobic cell growth on glucose carbon source	normal glucose MM, normal temp
gpt1 | alg6	slow aerobic cell growth on glucose carbon source	normal glucose MM, normal temp, agar plates
gpt1 | alg6	slow aerobic cell growth on glucose carbon source	normal glucose MM, high temp, agar plates
deletion alg6 | overexpressed gpt1	normal cell growth	plate, normal glucose MM, normal temp
deletion alg6 | overexpressed gpt1	normal cell growth	plate, normal glucose MM, high temp
gpt1|alg6	normal morphology	deletion, sorbitol added, normal glucose MM, liquid
gpt1|alg6	normal cell growth on gluocose carbon source	deletion, sorbitol added, normal glucose MM
deletion alg6 | overexpressed gpt1	normal morphology	normal glucose MM, high temp
deletion alg6| deletion gpt1|overexpression gpt1(Y1312A,D1352A)	round/swollen cells	normal temp, glucose MM
deletion alg6| deletion gpt1|overexpression gpt1(Y1312A,D1352A)	slow growth at high temp	high temp, glucose MM
gls2 | alg6	normal morphology	normal glucose MM, normal temp

11016847	​http://curation.pombase.org/pombe/curs/373f4c6a1e37adf9
yam8 | pck2	normal growth at high temp	allele=ehs1-1, allele=pck2(overexpressed),high temp, YES
yam8 | pck2	slow growth at high temp	allele=yam8delta, allele=pck2(overexpressed),high temp, YES
yam8 | pck2	normal conjugation freq	allele=ehs1-1, allele=pck2(overexpressed)
yam8 | pck2	decreased conjugation freq	allele=yam8delta, allele=pck2(overexpressed)
yam8 | pck2	normal growth on cilofungin	allele=ehs1-1, allele=pck2(overexpressed),normal temp, YES
yam8 | pck2	sensitive to cilofungin	allele=yam8delta, allele=pck2(overexpressed),normal temp, YES
yam8 | pck2	normal growth on calcofluor white	allele=ehs1-1, allele=pck2(overexpressed),normal temp, YES
yam8 | pck2	sensitive to calcofluor white	allele=yam8delta, allele=pck2(overexpressed),normal temp, YES
yam8 | pck2	slow growth	allele=pck2(overexpression),allele=ehs1-1, solid agar plates, glucose minimal medium, standard temperature
yam8 | pck2	normal cell growth	allele=pck2(overexpression),allele=ehs1-1, calcium excluded, solid agar plates, glucose minimal medium, standard temperature
yam8 | pck2	normal growth	allele=pck2(overexpression),allele=yam8delta, solid agar plates, glucose minimal medium, standard temperature
yam8 | pck2	normal growth	allele=pck2(overexpression),allele=yam8delta, calcium excluded, solid agar plates, glucose minimal medium, standard temperature
yam8 | pck2	normal level of calcium in cell	allele=ehs1-1, allele=pck2(overexpression), normal temp, glucose MM
yam8 | pck2	inviable	allele=ehs1-1, allele=pck2delta
yam8 | pck2	viable	allele=yam8delta, allele=pck2delta
yam8 | pck2	normal morphology	allele=pck2(overexpression),allele=yam8delta, glucose MM, standard temp microscopy
yam8 | pck2	lysed cells	allele=pck2(overexpression),allele=ehs1-1, glucose MM, standard temp microscopy
yam8 | pck1	sensitive to calcofluor white	allele=ehs1-1, allele=pck1(overexpressed),normal temp, YES
yam8 | pck1	sensitive to cilofungin	allele=ehs1-1, allele=pck1(overexpressed),normal temp, YES
yam8 | pck1	slow growth at high temp	allele=ehs1-1, allele=pck1(overexpressed),high temp, YES

12896976
pap1 | hba1	sensitive to caffeine	pap1 deleted, hba1 overexpressed, standard glucose MM, standard temp, agar plates
pap1 | hba1	sensitive to caffeine	pap1 deleted, hba1 deleted, standard glucose rich medium, standard temp, agar plates
sty1 | hba1	resistant to caffeine	sty1 deleted, hba1 deleted, standard glucose MM (yeah,diff from above), standard temp, agar plates

7565608	07cc7abfbaa6d1b0
rds1delta | ade6-D1	normal phenotype

18071249	​http://curation.pombase.org/pombe/curs/5bff0cacfd0d068a
lcf1 | lcf2	decreased myristoyl-ligase activity	double deletion
lcf1 | lcf2	abolished palmitoyl-ligase activity	double deletion
lcf1 | lcf2	abolished oleoyl-ligase activity	double deletion
lcf1 | lcf2	decreased viability in stationary phase	double deletion, standard glucose MM
lcf1 | lcf2	decreased viability in stationary phase	double deletion, growth to stationary phase in rich medium followed by transfer to H2O
lcf1 | lcf2	decreased viability in stationary phase	double deletion, growth to stationary phase in rich medium followed by transfer to H2O
lcf1 | lcf2	decreased cell density in stationary phase	allele= double deletion, growth to stationary phase in rich medium followed by transfer to minimal medium

21811607	​http://curation.pombase.org/pombe/curs/db6b03f9221f5e4a/
pkd2 | pmr1	normal immediate intracellular calcium spike following extracellular calcium stimulus	enzyme assay,allele=overexpression pkd2 / deletion(pmr1)
pkd2 | pmc1	normal immediate intracellular calcium spike following extracellular calcium stimulus	enzyme assay,allele=overexpression pkd2 / deletion(pmc1)
pkd2 | vcx1	normal immediate intracellular calcium spike following extracellular calcium stimulus	enzyme assay,allele=overexpression pkd2 / deletion(vcx1)
pek1 | cch1	normal calcium level in response to salt stress	pek1DD(unknown), overexpression, cch1delta, enzyme assay
pek1 | yam8	normal calcium level in response to salt stress	pek1DD(unknown), overexpression, yam8delta, enzyme assay
pek1 | yam8	normal calcium level in response to salt stress	pek1DD(unknown), overexpression, yam8delta, FK506 added, enzyme assay
1322 | ppb1	normal cellular response to salt stress	overexpression of 1322 and deletion of ppb1, cell growth assay, Na ions OR magnesium ions added
1322|prz1	decreased immediate intracellular calcium spike following extracellular calcium stimulus	1322 overexpressed, prz1delta
1322|ppb1	absent immediate intracellular calcium spike following extracellular calcium stimulus	1322 overexpressed, ppb1delta
pkd2|prz1	decreased immediate intracellular calcium spike following extracellular calcium stimulus	pkd2 overexpressed, prz1delta
pkd2|prz1	decreased immediate intracellular calcium spike following extracellular calcium stimulus	pkd2 overexpressed, ppb1delta
1322|pek1	normal cellular calcium level during salt stress	1322-delta, pek1DD(unknown)
1322|pek1	normal cellular calcium level during salt stress	1322-delta, pek1DD(unknown), tacrolimus added
trp1322 | pmr1	normal prolonged cellular calcium level	1322overexpression, pmr1delta, enzyme assay
pkd2 | pmr1	normal prolonged cellular calcium level	pkd2overexpression, pmr1delta, enzyme assay
yam8 | cch1	increased cellular calcium level in response to sodium ions	doubledeletion, enzyme assay

9573052
pom1|cdc25	monopolar actin cortical patch localization	allele=pom1delta, allele=cdc25-22, rhodamine-phalloidin added, high temp
pom1|cdc10	monopolar actin cortical patch localization to either end	allele=pom1delta, allele=cdc10-V50, rhodamine-phalloidin added, high temp
pom1|cdc25	activation of monopolar cell growth at either end	allele=pom1delta, allele=cdc25-22, rhodamine-phalloidin added, high temp
pom1|cdc10	activation of monopolar cell growth at either end	allele=pom1delta, allele=cdc10-V50, rhodamine-phalloidin added, high temp
pom1|cdc11	branched cells	pom1delta,cdc11-136, high temp, calcofluor added
pom1|act1	slow growth	pom1delta, act1-48, at high, low and standard temp
pom1|cdc3	slow growth	pom1delta, cdc3-6, low temp, standard temp
pom1|cdc8	slow growth	pom1delta, cdc8-110, low temp, standard temp
pom1|nda3	slow growth	pom1delta, nda3-KM311, standard temp
pom1|ban5	slow growth	pom1delta, ban5-3, high temp, standard temp
pom1|cdc14	slow growth	pom1delta, cdc14-118
pom1|mid1	slow growth	pom1delta, dmf1-6
pom1|tea1	viable	pom1delta, tea1delta, high temp, standard temp, low temp
pom1|tea1	branched cells	pom1delta, tea1delta, high temp, standard temp, low temp
pom1|tea1	mislocalized septum	pom1delta, tea1delta

10224084	​http://curation.pombase.org/pombe/curs/169c0f8873eee853
hmt2|sir1	normal cellular acid labile sulfide (S2-) level	hmt2JS563(E396K)|sir1delta, rich medium

10223994	​http://curation.pombase.org/pombe/curs/b0116284c3e493a0
tps1|ntp1	resistant to freeze-thawing	allele=tps1(overexpressed),ntp1(deletion), EMM, solid agar, growth to exponential phase
tps1|ntp1	resistant to dehydration	allele=tps1(overexpressed),ntp1(deletion), EMM, solid agar, growth to exponential phase
tps1|ntp1	resistant to ethanol	allele=tps1(overexpressed),ntp1(deletion), EMM, solid agar, growth to exponential phase
tps1|ntp1	increased trehalose level during veg growth	allele=tps1(overexpressed),ntp1(deletion), MM, growth to exponential phase
tps1|ntp1	increased trehalose level in response to heat shock	allele=tps1(overexpressed),ntp1(deletion), MM, heat shock 30 mins 48 degrees, growth to exponential phase
tps1|ntp1	resistance to heat shock	CGA, allele=tps1(overexpressed),ntp1(deletion), MM, heat shock 30 mins 48 degrees, liquid culture, growth to exponential phase

9532803	​http://curation.pombase.org/pombe/curs/a826f97af4e46f8f
pap1|rpn11	sensitive to brefeldin A	pap1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|rpn11	normal response to staurosporine	pap1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|rpn11	sensitive to K-252a	pap1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|rpn11	sensitive to actinomycin D	pap1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|rpn11	sensitive to cytochalasin B	pap1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|bfr1	resistant to brefeldin A	pap1delta, bfr1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|bfr1	resistant to staurosporine	pap1delta, bfr1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|bfr1	resistant to K-252a	pap1delta, bfr1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|bfr1	resistant to actinomycin D	pap1delta, bfr1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|bfr1	resistant to cytochalasin B	pap1delta, bfr1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|rpn11	sensitive to brefeldin A	bfr1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|rpn11	resistant to staurosporine	bfr1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|rpn11	sensitive to K-252a	bfr1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|rpn11	sensitive to actinomycin D	bfr1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|rpn11	sensitive to cytochalasin B	bfr1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|pap1	sensitive to brefeldin A	bfr1delta, pap1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|pap1	resistant to staurosporine	bfr1delta, pap1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|pap1	sensitive to K-252a	bfr1delta, pap1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|pap1	sensitive to actinomycin D	bfr1delta, pap1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|pap1	normal cellular response to cytochalasin B	bfr1delta, pap1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added

16262791	|​http://curation.pombase.org/pombe/curs/722def8c5161e3f1/
tsc2|rhb1	normal cellular response to canavanine	allele=tsc2delta,rhb1(D60K), CGA, agar plates, emm

18637840	​http://curation.pombase.org/pombe/curs/b719dc2cd003f460
zrt1 | zhf1	reduced cellular zinc level	liquid scintillation counter/AAS/substance quantification, both deleted, EMM, zinc added
zrt1 | zhf1	sensitive to zinc and zinc starvation	both deleted, liquid, standard temp
zrt1 | zhf1	sensitive to zinc and zinc starvation	both deleted, agar, standard temp, emm
zrt1 | zhf1	normal cellular copper level	spectroscopy, EMM, zinc added, both deleted
zrt1 | zhf1	normal cellular potassium level	spectroscopy, EMM, zinc added, both deleted
zrt1 | zhf1	normal cellular iron level	spectroscopy, EMM, zinc added, both deleted
zrt1 | zhf1	normal cellular manganese level	spectroscopy, EMM, zinc added, both deleted
zrt1 | zhf1	normal cellular magnesium level	spectroscopy, EMM, zinc added, both deleted
zrt1 | zhf1	normal cellular molybdenum level	spectroscopy, EMM, zinc added, both deleted
zrt1 | zhf1	normal cellular nickel level	spectroscopy, EMM, zinc added, both deleted
zrt1 | zhf1	normal cellular phosphorus level	spectroscopy, EMM, zinc added, both deleted
zrt1 | zhf1	normal cellular sulfur level	spectroscopy, EMM, zinc added, both deleted
zrt1 |spcc126.09	sensitive to zinc starvation	allele=deletion, emm, limited zinc added

18662319	​http://curation.pombase.org/pombe/curs/6dc480135e507182/
cys11 | pgt1	normal cell growth	both deleted, emm, plates, cysteine added
cys11 | isp4	normal cell growth	both deleted, emm, plates, cysteine added
cys11 | spcc1840.12	normal cell growth	both deleted, emm, plates, cysteine added	|
cys11 | pgt1	inviable veg cells	both deleted, emm, plates, GSH added
cys11 | isp4	normal cell growth on glucose carbon source	both deleted, emm, plates, GSH added
cys11 | spcc1840.12	normal cell growth on glucose carbon source	both deleted, emm, plates, GSH added
cys11 | pgt1	decreased cellular glutathione level	both deleted, limited sulphur added, GSH added

9852154	​http://curation.pombase.org/pombe/curs/67631dafe77debd1
cdc25-22|pom1-delta1	normal protein localization	microscopy, emm, high temp, annotation_extensino=assayed_using(PomBase:SPAP8A3.08)
cdc25-22|mid1-deltaF	abnormal protein localization	microscopy, emm, high temp, annotation_extensino=assayed_using(PomBase:SPAP8A3.08)
cdc25-22|mid1-deltaF	abnormal contractile ring localization	microscopy, emm, high temp, annotation_extensino=assayed_using(PomBase:SPAP8A3.08)

plo1-1|mid1-deltaF	decreased cell growth	CGA, YES, low temp, agar
plo1-1|mid1-deltaF	decreased cell growth	CGA, YES, standard temp, agar
plo1-1|mid1-deltaF	decreased cell growth	CGA, YES, high temp, agar

plo1-24c|mid1-deltaF	decreased cell growth	CGA, YES, low temp, agar
plo1-24c|mid1-deltaF	decreased cell growth	CGA, YES, standard temp, agar
plo1-24c|mid1-deltaF	decreased cell growth	CGA, YES, high temp, agar

pom1-1|mid1-deltaF	inviable germinating spore, no cell divisions	CGA, YES, low temp, agar
pom1-1|mid1-deltaF	inviable germinating spore, no cell divisions	CGA, YES, standard temp, agar
pom1-1|mid1-deltaF	inviable germinating spore, no cell divisions	CGA, YES, high temp, agar

pom1-1|plo1-1	decreased cell growth	CGA, YES, low temp, agar
pom1-1|plo1-1	decreased cell growth	CGA, YES, standard temp, agar
pom1-1|plo1-1	inviable	CGA, YES, high temp, agar

pom1-1|plo1-24c	normal cell growth	CGA, YES, low temp, agar
pom1-1|plo1-24c	decreased cell growth	CGA, YES, standard temp, agar
pom1-1|plo1-24c	inviable	CGA, YES, high temp, agar

22840777	​http://curation.pombase.org/pombe/curs/f94801dfb551d839
spbc530.05| pap1	decreased RNA level during cellular response to purvalanol A	deletion, quant pcr, assayed using bfr1

22840777	​http://curation.pombase.org/pombe/curs/f94801dfb551d839
prt1delta|bfr1delta	sensitive to BFA	yes, agar, standard temp
prt1delta|bfr1delta	sensitive to cycloheximide	yes, agar, standard temp
prt1delta|bfr1delta	sensitive to purA	yes, agar, standard temp
bfr1 | SPAC17C9.16c| sensitive to BFA	deletion, agar, normal temp, yes
bfr1 | SPBC36.03c| sensitive to BFA	deletion, agar, normal temp, yes
bfr1 | SPAC17C9.16c| sensitive to cycloheximide	deletion, agar, normal temp, yes
bfr1 | SPBC36.03c| sensitive to cycloheximide	deletion, agar, normal temp, yes
bfr1 | SPAC17C9.16c| sensitive to purA	deletion, agar, normal temp, yes
bfr1 | SPBC36.03c| sensitive to purA	deletion, agar, normal temp, yes
caf5 | SPAC17C9.16c	sensitive to purvalanol A	deletion, yes, agar, standard temp
caf5 | SPAC17C9.16c	sensitive to cycloheximide	deletion, yes, agar, standard temp
caf5 | SPAC17C9.16c	sensitive to BFA	deletion, yes, agar, standard temp

prt1delta| pap1delta | bfr1delta | pap1 overexpressed	resistance to BFA	CGA, EMM, agar, normal temp
prt1delta| pap1delta | bfr1delta | SPAC22A12.16 overexpressed	resistance to BFA	CGA, EMM, agar, normal temp
prt1delta| pap1delta | bfr1delta | SPAC17C9.16c overexpressed	resistance to BFA	CGA, EMM, agar, normal temp
prt1delta| pap1delta | bfr1delta | caf5 overexpressed	resistance to BFA	CGA, EMM, agar, normal temp
prt1delta| pap1delta | bfr1delta | tcg1 overexpressed	resistance to BFA	CGA, EMM, agar, normal temp
prt1delta| pap1delta | bfr1delta | glo3 overexpressed	resistance to BFA	CGA, EMM, agar, normal temp
prt1delta| pap1delta | bfr1delta | SPAC2212.06c overexpressed	resistance to BFA	CGA, EMM, agar, normal temp
prt1delta| pap1delta | bfr1delta | pmc2 overexpressed	resistance to BFA	CGA, EMM, agar, normal temp
prt1delta| pap1delta | bfr1delta | cct8 overexpressed	resistance to BFA	CGA, EMM, agar, normal temp
prt1delta| pap1delta | bfr1delta | SPAC12G12.07c overexpressed	resistance to BFA	CGA, EMM, agar, normal temp
prt1delta| pap1delta | bfr1delta | SPBC839.14c overexpressed	resistance to BFA	CGA, EMM, agar, normal temp
prt1delta| pap1delta | bfr1delta | tdh1 overexpressed	resistance to BFA	CGA, EMM, agar, normal temp
prt1delta| pap1delta | bfr1delta | cpc2 overexpressed	resistance to BFA	CGA, EMM, agar, normal temp

bfr1 | pmd1 | SPAC17C9.16c | caf5	normal cell growth	deletion, yes, standard temp, agar
bfr1 | pmd1 | SPAC17C9.16c | caf5	sensitive to brefeldin A	deletion, yes, standard temp, agar
bfr1 | pmd1| SPAC17C9.16c | caf5	sensitive to CHX	deletion, yes, standard temp, agar
bfr1 | pmd1| SPAC17C9.16c | caf5	sensitive to pur A	deletion, yes, standard temp, agar

prt1| pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	sensitive to brefeldin A	deletion, yes, standard temp, agar
prt1| pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5	sensitive to CHX	deletion, yes, standard temp, agar
prt1| pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5	sensitive to our A	deletion, yes, standard temp, agar

pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	sensitive to brefeldin A	allele=MDR-sup(deletion), yes, standard temp, agar
pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5	sensitive to CHX	MDR-sup,deletion, yes, standard temp, agar
pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5	sensitive to pur A	MDR-sup, deletion, yes, standard temp, agar
pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	sensitive to brefeldin A	allele=MDR-sup(deletion), EMM, standard temp, liquid
pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5	sensitive to CHX	MDR-sup,deletion, EMM, standard temp, liquid
pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5	sensitive to pur A	MDR-sup, deletion, EMM, standard temp, liquid
pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	sensitive to brefeldin A	allele=MDR-sup(deletion), EMM, standard temp, agar
pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5	sensitive to CHX	MDR-sup,deletion, EMM, standard temp, agar
pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5	sensitive to pur A	MDR-sup, deletion, EMM, standard temp, agar

pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	sensitive to latrunculin A (actin assembly inhibitor)	allele=MDR-sup(deletion), yes, standard temp, liquid
pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	sensitive to reversine (cell cycle kinase inhibitor)	allele=MDR-sup(deletion), yes, standard temp, liquid
pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	sensitive to hesperadin (cell cycle kinase inhibitor)	allele=MDR-sup(deletion), yes, standard temp, liquid
pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	normal cellular response to MBC (tubulin poison)	allele=MDR-sup(deletion), yes, standard temp, liquid
pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	normal cellular response to benomyl (tubulin poison)	allele=MDR-sup(deletion), yes, standard temp, liquid

prt1| pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	sensitive to cold	deletion, yes, low temp, agar
prt1| pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5	sensitive to heat	deletion, yes, high temp, agar
prt1| pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5	normal cell growth	deletion, yes, normal temp, agar

pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	normal growth	allele=MDR-sup(deletion), yes, standard temp, agar
pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	normal growth at high temp	allele=MDR-sup(deletion), yes, high temp, agar
pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	normal growth at low temp	allele=MDR-sup(deletion), yes, low temp, agar
pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	normal growth	allele=MDR-sup(deletion), yes, standard temp, liquid
pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	normal growth at high temp	allele=MDR-sup(deletion), yes, high temp, liquid
pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	normal growth at low temp	allele=MDR-sup(deletion), yes, low temp, liquid
pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	normal growth	allele=MDR-sup(deletion), EMM, standard temp, liquid

pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	normal sporulation	allele=MDR-sup(deletion), standard temp
pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	normal cell cycle progressionn	allele=MDR-sup(deletion), standard temp

bfr1 | pmd1 | SPAC17C9.16c | caf5	sensitive to brefeldin A	deletion, yes, standard temp, liquid culture
bfr1 | pmd1| SPAC17C9.16c | caf5	sensitive to CHX	deletion, yes, standard temp, liquid culture
bfr1 | pmd1| SPAC17C9.16c | caf5	sensitive to pur A	deletion, yes, standard temp, liquid culture

prt1| pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	sensitive to brefeldin A	deletion, yes, standard temp, liquid culture
prt1| pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5	sensitive to CHX	deletion, yes, standard temp, liquid culture
prt1| pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5	sensitive to our A	deletion, yes, standard temp, liquid culture

pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	sensitive to brefeldin A	MDR-sup, deletion, yes, standard temp, liquid culture
pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5	sensitive to CHX	MDR-sup, deletion, yes, standard temp, liquid culture
pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5	sensitive to our A	MDR-sup, deletion, yes, standard temp, liquid culture
caf5 | SPAC17C9.16c | pmd1 | bfr1	sensitive to to brefeldin A	deletion, yes, standard temp, agar

These requests may change depending on the SF item outcome because it is a screen against many compounds:

pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	sensitive to protease inhibitors	MDR-sup, deletion
pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	sensitive to kinase inhibitors	MDR-sup, deletion
pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	sensitive to topoisomerase inhibitors	MDR-sup, deletion
pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	sensitive to capsazepine (a vanilloid receptor antagonist) inhibitors	MDR-sup, deletion
pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	sensitive to PPT (an estrogen receptor agonist)	MDR-sup, deletion
pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	sensitive to GW7647 (a receptor agonist)	MDR-sup, deletion

pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	sensitive to velcade	MDR-sup, deletion, yes, liquid, standard temp
pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	sensitive to nocodazole (an inhibitor of microtubule polymerisation)	MDR-sup, deletion, yes, liquid, standard temp

pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	cell cycle arrest in metaphase	MDR-sup, deletion, velcade added
pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	abolished mitotic spindle assembly	MDR-sup, deletion, velcade and nocodazole added
pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5	clustered spindle pole bodies	MDR-sup, deletion, velcade and nocodazole added

22139915	​http://curation.pombase.org/pombe/curs/88220312b9269e43
rap1| rpb7	decreased RNA level	allele=deletion, allele=rpb7-G150D, hight temp, assayed_using(TERRA), northern blot

19185548	​http://curation.pombase.org/pombe/curs/025da8633ca22521
SPAC17A2.12 | rad51	sensitive to MMS	dbl-deletion, agar, yes, CGA
SPBC23E6.02 | rad51	sensitive to MMS	dbl-deletion, agar, yes, CGA
SPAC17A2.12 | rad51	sensitive to MMS	dbl-deletion, liquid, yes, CGA
SPBC23E6.02 | rad51	sensitive to MMS	dbl-deletion, liquid, yes, CGA
SPAC17A2.12 | rad51	sensitive to CPT	dbl-deletion, agar, yes, CGA
SPBC23E6.02 | rad51	sensitive to CPT	dbl-deletion, agar, yes, CGA
SPAC17A2.12 | rad51	normal cell growth on glucose carbon source	dbl-deletion, agar, yes, CGA
SPBC23E6.02 | rad51	normal cell growth on glucose carbon source	dbl-deletion, agar, yes, CGA

SPAC17A2.12 | SPBC23E6.02	normal cell growth on glucose carbon source	dbl-deletion, agar, yes, CGA
SPAC17A2.12 | SPBC23E6.02	normal cell growth on MMS	dbl-deletion, agar, yes, CGA
SPAC17A2.12 | SPBC23E6.02	normal cell growth on CPT	dbl-deletion, agar, yes, CGA

SPAC17A2.12 | sfr1	normal cell growth on glucose carbon source	dbl-deletion, agar, yes, CGA
SPBC23E6.02 | sfr1	normal cell growth on glucose carbon source	dbl-deletion, agar, yes, CGA
SPAC17A2.12 | SPBC23E6.02 | sfr1	normal cell growth on glucose carbon source	trpl-deletion, agar, yes, CGA
SPAC17A2.12 | sfr1	sensitive to CPT	dbl-deletion, agar, yes, CGA
SPBC23E6.02 | sfr1	sensitive to CPT	dbl-deletion, agar, yes, CGA
SPAC17A2.12 | SPBC23E6.02 | sfr1	normal cell growth on CPT	dbl-deletion, agar, yes, CGA, this is really what it showed

SPAC17A2.12 | rad57	normal cell growth on glucose carbon source	dbl-deletion, agar, yes, CGA
SPBC23E6.02 | rad57	normal cell growth on glucose carbon source	dbl-deletion, agar, yes, CGA
SPAC17A2.12 | SPBC23E6.02 | rad57	normal cell growth on glucose carbon source	trpl-deletion, agar, yes, CGA
SPAC17A2.12 | rad57	sensitive to MMS	dbl-deletion, agar, yes, CGA
SPBC23E6.02 | rad57	sensitive to MMS	dbl-deletion, agar, yes, CGA
SPAC17A2.12 | rad57	sensitive to MMS	dbl-deletion, liquid, yes, CGA
SPBC23E6.02 | rad57	sensitive to MMS	dbl-deletion, liquid, yes, CGA
SPAC17A2.12 | SPBC23E6.02 | rad57	sensitive to MMS	trpl-deletion, agar, yes, CGA
SPAC17A2.12 | rad57	sensitive to CPT	dbl-deletion, agar, yes, CGA
SPBC23E6.02 | rad57	sensitive to CPT	dbl-deletion, agar, yes, CGA
SPAC17A2.12 | SPBC23E6.02 | rad57	sensitive to CPT	trpl-deletion, agar, yes, CGA
SPAC17A2.12 | rad57	sensitive to HU	dbl-deletion, agar, yes, CGA
SPBC23E6.02 | rad57	sensitive to HU	dbl-deletion, agar, yes, CGA
SPAC17A2.12 | rad57	normal cellular response to HU	SPAC17A2.12: over expressed, rad57:deleted, agar, yes, CGA
SPAC17A2.12 | SPBC23E6.02 | rad57	sensitive to HU	trpl-deletion, agar, yes, CGA

sfr1 | rhp57	elongated viable vegetative cells	deletion, microscopy
sfr1 | rhp57	abnormal nuclear morphology	deletion, microscopy
SPAC17A2.12 | sfr1 | rhp57	elongated viable vegetative cells	deletion, microscopy
SPBC23E6.02 | sfr1 | rhp57	elongated viable vegetative cells	deletion, microscopy
SPAC17A2.12 | rhp57	elongated viable vegetative cells	deletion, microscopy
SPBC23E6.02 | rhp57	elongated viable vegetative cells	deletion, microscopy
SPAC17A2.12 | rhp57	abnormal nuclear morphology	deletion, microscopy
SPBC23E6.02 | rhp57	abnormal nuclear morphology	deletion, microscopy
SPAC17A2.12 | sfr1	normal cell size	deletion microscopy
SPBC23E6.02 | sfr1	normal cell size	deletion microscopy

rhp57 | SPAC17A2.12	increased DNA damage checkpoint activation during non stressed conditions	deletion, western, annotation_extension=assayed_using(PomBase:SPCC1259.13)
rhp57 | SPBC23E6.02	increased DNA damage checkpoint activation during non stressed conditions	deletion, western, annotation_extension=assayed_using(PomBase:SPCC1259.13)
SPAC17A2.12 | SPBC23E6.02	normal DNA damage checkpoint activation during non stressed conditions	deletion, western, annotation_extension=assayed_using(PomBase:SPCC1259.13)
rhp57 | SPAC17A2.12	normal DNA damage checkpoint activation during gamma irradiation	deletion, western, annotation_extension=assayed_using(PomBase:SPCC1259.13)
rhp57 | SPBC23E6.02	normal DNA damage checkpoint activation during gamma irradiation	deletion, western, annotation_extension=assayed_using(PomBase:SPCC1259.13)
SPAC17A2.12 | SPBC23E6.02	normal DNA damage checkpoint activation during gamma irradiation	deletion, western, annotation_extension=assayed_using(PomBase:SPCC1259.13)
rhp57 | SPAC17A2.12	slow cell growth rate (FYPO:0001234)	deletion, CGA
rhp57 | SPBC23E6.02	slow cell growth rate (FYPO:0001234)	deletion, CGA

15075270	​http://curation.pombase.org/pombe/curs/9a4ed1603d089abe
liz1 | pan6	inviable	deletion, CGA, pantothenate excluded, glucose MM, agar, normal temp
liz1 | pan6	inviable	deletion, CGA, limited pantothenate added, glucose MM, agar, normal temp
liz1 | pan6	normal growth	deletion, CGA, pantothenate added, glucose MM, agar, normal temp

16299000	​http://curation.pombase.org/pombe/curs/110f413a16dde4e4
sod1|phx1	increased protein level	sod 1 knockdown, phx1-OP, western, annotation_extension=assayed_using(PomBase:SPBC1105.02c)
sod1|lys4	normal growth	liquid emm, sod1 knockdown, lys4 OP
sod1|lys4	normal growth	agar emm, cysteine added, sod1 knockdown, lys4 OP
sod1|lys4	decreased growth	agar emm, lysine added, sod1 knockdown, lys4 OP

23074192	​http://curation.pombase.org/pombe/curs/4a7f9665ed7386e8/
SPAC17D4.04 - trm4a | SPAC23C4.17 - trm4b	decreased tRNA methylation	deletion, trna asp residues C48, C49, C60, C61, C62
SPAC17D4.04 - trm4a | SPAC23C4.17 - trm4b	increased tRNA methylation	deletion, trna asp residues C38
pmt1 | trm4b | trm4a	viable	CGA

12376568	​http://curation.pombase.org/pombe/curs/4d6014a904a1e84b
ned1|crm1	normal growth on glucose carbon source	ned1-1, crm1-809, agar, yes, cga
ned1|crm1	normal cell growth on staurosporine	ned1-1, crm1-809, agar, yes, cga
ned1|crm1	normal cell growth on caffeine	ned1-1, crm1-809, agar, yes, cga
ned1|crm1	normal protein level	ned1-1, crm1-809, annotation_extension=assayed_using(PomBase:SPAC3C7.14c)
ned1|pim1	inviable	ned1-1, pim1-46, agar
ned1|pim1	normal cell growth on tbz	ned1-1, pim1 OP, agar, yes
ned1|spi1	normal cell growth on tbz	ned1-1, spi1-OP, agar, yes
ned1|dis1	sensitive to cold	ned1-1, dis1 delta, agar
ned1|dis2	sensitive to cold	ned1-1, dis2-11, agar
ned1|dis3	sensitive to cold	ned1-1, dis3-54, agar
ned1|dis1	normal growth on glucose carbon source	ned1-1, dis1 delta, agar, high temp
ned1|dis2	normal growth on glucose carbon source	ned1-1, dis2-11, agar, high temp
ned1|dis3	normal growth on glucose carbon source	ned1-1, dis3-54, agar, high temp

9135147	​http://oliver0.sysbiol.cam.ac.uk/pombe/curs/67ba83cbaba024a7
pmk1 | spk1	sterile	allele=pmk1delta(deletion), allele=spk1delta(deletion)
pmk1 | spk1	abnormal morphology	allele=pmk1delta(deletion), allele=spk1delta(deletion), condition=PCO:0000014, condition=PCO:0000102
pmk1 | spk1	normal response to salt stress	allele=pmk1delta(deletion), allele=spk1delta(deletion), condition=PCO:0000014, condition=PCO:0000102
wis1 | pmk1	multiseptate	microscopy, allele=wi1delta(deletion), allele=pmk1delta(deletion), condition=PCO:0000014, condition=PCO:0000102

11071923	​http://curation.pombase.org/pombe/curs/6a65148752d79293
int6	elongated in response to caffeine	wt int6 expressed from endogenous promoter + deletion of last 50 a.a's (452-501)=Cdelta50
int6	viable in response to caffeine	wt int6 expressed from endogenous promoter + overexpression of a mutant with deletion of last 50 a.a's (452-501)=Cdelta50

14008	​http://curation.pombase.org/pombe/curs/cc0a2203f689cfc8
apt1 | hpt1	increased level of 5-Phosphoribosyl diphosphate CHEBI:17111	dap1/pur1 (unknown), substance quant
hpt1 | ade1	decreased guanine import	pur1, ade1 (unknown) substance quant
hpt1 | apt1	decreased adenine import	pur1, (unknown) substance quant
hpt1 | apt1	decreased guanine import	pur1, (unknown) substance quant

17346842	​http://curation.pombase.org/pombe/curs/b719dc2cd003f460
stm1 | ras1	decreased growth	stm OP, ras1-delta, CGA, liquid
stm1 | ste11	decreased growth	stm OP, ste11-delta, CGA, liquid
stm1 | sty1	normal growth	stm OP, sty1-delta, CGA, liquid
gpa2 | sty1	normal growth	stm OP, sty1-delta, CGA, liquid

9090050	​http://curation.pombase.org/pombe/curs/39a5a6a1083e818a
ght1 | ght5 | ght6	abolished glucose import	YGS-5(all deleted)
ght1 | ght5 | ght6	normal cell growth on gluconate carbon source	YGS-5(all deleted)
ght1 | ght5 | ght6	inviable	YGS-5(all deleted), glucose/peptone/ye medium, standard temp, agar

8000531	​http://curation.pombase.org/pombe/curs/cb152da725a506ef
ght1 | ght5 | ght6	abolished glucose import	YGS-5(all deleted)
ght1 | ght5 | ght6	abolished fructose import	YGS-5(all deleted)
ght1 | ght5 | ght6	decreased 2-deoxy-D-glucose import	YGS-5(all deleted)

10735857	​http://curation.pombase.org/pombe/curs/495144e748000da3
ght3 | ght4	inviable on gluconate carbon source	double deletion
ght1 | ght5 | ght6	increased RNA level during growth on glucose	YGS-5(all deleted), assayed_using(ght3)|assayed_using(ght4), northern

1899230	​http://curation.pombase.org/pombe/curs/b047e0c4a7554079
pap1|spk1	sensitive to staurosporine	pap1-delta, spk1-OP, CGA
pap1|sts1	sensitive to staurosporine	pap1-OP, sts1-5, CGA
spk1|sts1	sensitive to staurosporine	spk1-OP, sts1-5, CGA
pap1|sts5	normal growth on staurosporine	pap1-OP, sts5-7, CGA
spk1|sts5	normal growth on staurosporine	spk1-OP, sts5-7, CGA
pap1|pck2	normal growth on staurosporine	pap1-OP, sts6-8, CGA
spk1|pck2	normal growth on staurosporine	spk1-OP, sts6-8, CGA

1833395	​http://curation.pombase.org/pombe/curs/0b858a6efa067b13
pma1|pma2	decreased ATPase activity	pma2-op, pma1-delta, enzyme assay
pma1|pma2	normal growth on acidic medium/lowpH	pma2-op, pma1-delta, enzyme assay
pma1|pma2	normal growth on acidic medium/lowpH	pma2-op, pma1-1(unknown), enzyme assay

9693066	​http://oliver0.sysbiol.cam.ac.uk/pombe/curs/3e8456a6d6a5d6a0
fas1 | fas2	increased fatty acid synthase activity	both overexpressed, enzyme assay

14665462	​http://curation.pombase.org/pombe/curs/f45b0b374b31905c
sla1 | ras1	sterile	ras1-delta, sla1-delta, PM agar + nitrogen
sla1 | ras1	normal conjugation	ras1-delta, sla1deltaC(truncated C-term, not specified what residues)

22416758	​http://curation.pombase.org/pombe/curs/1eb406bf7346ff24
mpg1 mpg2	increased septation index	mpg1 knockdown, mpg2-delta, annotation_extension=has_penetrance(FYPO_EXT:0000001), micro
mpg1 mpg2	binucleate, monoseptate cells	mpg1 knockdown, mpg2-delta, annotation_extension=has_penetrance(FYPO_EXT:0000001), micro
mpg1 mpg2	DNA content increased	mpg1 knockdown, mpg2-delta, micro

16049679	​http://curation.pombase.org/pombe/curs/eb090de8a5e6eecf
mpg1 wee-1	multinucleate with septum betwen cells	knockdown mpg1, wee1-50, high temp, micro
mpg1 cdc25	abnormal cytokinetic cell separation	knockdown mpg1, cdc25-22, microscopy
mpg1 cdc25	multinucleate with septum betwen cells	knockdown mpg1, cdc25-22, microscopy

7769002	​http://curation.pombase.org/pombe/curs/54de4db66c7dfe2e
pap1 | rpn11	decreased RNA level during veg growth	pap1-delta, rpn11-OP, transcript expr
pap1 | rpn11	decreased protein level during veg growth	pap1-delta, rpn11-OP, western
obr1 | rpn11	decreased protein level during veg growth	obr1 deletion of AP-1 TF binding site, rpn11-OP, western
obr1 | rpn11	decreased protein level during veg growth	obr1 deletion of PLD TF binding site, rpn11-OP, western

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