wiki:AntoniasDoubleMutants2

Version 41 (modified by antonialock, 9 years ago) (diff)

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https://sourceforge.net/apps/trac/pombase/wiki/AntoniasCuratedPapers

https://sourceforge.net/apps/trac/pombase/wiki/AntoniasDoubleMutants


2038319http://curation.pombase.org/pombe/curs/468db0c3950bdbf6
ste6 | gap1 elongated during nitrogen starvationste6-delta, gap1-delta
ral2 | gap1 elongated d


8804400http://curation.pombase.org/pombe/curs/d8d3bfcf3cf532c6
git5 | gpa1conjugation abolisheddouble deletion, cga
git5 | ras1spheroid cellsdouble deletion, EMM, cga
git5 | ras1abolished conjugationdouble deletion, EMM, cga
git5 | ras1spheroid cellsgit5-OP, ras1-delta, EMM, cga
git5 | ras1abolished conjugationgit5-OP, ras1-delta, EMM, cga
git5 | gpa2increased conjugationdouble deletion, MEA or YE, cga
git5 | gpa2increased sporulationdouble deletion, MEA or YE, cga


8887550http://curation.pombase.org/pombe/curs/0e9fdcae51e973b7
cwg2 | cdc42cell lysis cwg2-1, cdc42-OP, high temp
cwg2 | cdc42cell lysis cwg2-1, cdc42G12V-OP, high temp|
cwg2 | cdc42cell lysis cwg2-1, cdc42Q61L-OP, high temp|
cwg2 | rho1cell lysis cwg2-1, rho1-OP, high temp
cwg2 | rho1cell lysis cwg2-1, rho1G15V-OP, high temp
cwg2 | rho1cell lysis cwg2-1, rho1Q64L-OP, high temp
cwg2 | cdc42decreased (1-3)f-D-glucan synthase activity cwg2-1, cdc42-OP, high temp, EMM, sorbitol added, GTP added
cwg2 | cdc42decreased (1-3)f-D-glucan synthase activity cwg2-1, cdc42G12V-OP, high temp, EMM, sorbitol added, GTP added
cwg2 | cdc42decreased (1-3)f-D-glucan synthase activity cwg2-1, cdc42Q61L-OP, high temp, EMM, sorbitol added, GTP added
cwg2 | rho1increased (1-3)f-D-glucan synthase activity cwg2-1, rho1-OP, high temp, EMM, sorbitol added, GTP added
cwg2 | rho1increased (1-3)f-D-glucan synthase activity cwg2-1, rho1G15V-OP, high temp, EMM, sorbitol added, GTP added
cwg2 | rho1increased (1-3)f-D-glucan synthase activity cwg2-1, rho1Q64L-OP, high temp, EMM, sorbitol added, GTP added
cwg2 | rho1increased (1-3)f-D-glucan synthase activity cwg2-1, rho1G15V-OP, high temp, EMM, sorbitol added
cwg2 | rho1increased (1-3)f-D-glucan synthase activity cwg2-1, rho1Q64L-OP, high temp, EMM, sorbitol added


8887552http://curation.pombase.org/pombe/curs/f1afdd21aae916be
cdc2 | spd1 small vegetative cells (wee) cdc2-3w, spd1-delta, microscopy
cdc2 | spd1 small vegetative cells (wee) cdc2-3w, spd1-delta
cig2 | spd1 normal cell shape cig2-delta, spd1-delta, microscopy
cig2 | spd1 normal cell size cig2-delta, spd1-delta
cdc10 | spd1 normal aerobic growth on glucose cdc10-129, spd1-delta, cga, standard temp, agar
cdc10 | spd1 normal aerobic growth on glucose cdc10-129, spd1-delta, cga, standard temp, agar
cdc10 | spd1 normal aerobic growth on glucose cdc10-129, spd1-delta, cga, high temp, agar
cdc10 | spd1 normal aerobic growth on glucose cdc10-129, spd1-delta, cga, high temp, agar


8887647http://curation.pombase.org/pombe/curs/ec1e2e8292ae5f6e
mal2| nda2inviable, spores germinate but cannot dividemal2-1, nda2-KM52, CGA, high penetrance
mal2| nda2inviable, multiple cell divisionsmal2-1, nda2-KM52, CGA, low penetrance
mal2| nda3normal cell growthmal2-1, nda3-KM311, CGA, standard temp
mal2| nda3sensitive to high tempmal2-1, nda3-KM311, CGA
mal2| nda3sensitive to low tempmal2-1, nda3-KM311, CGA


8887553http://curation.pombase.org/pombe/curs/c5bcc1ff40b42c22
cdc1 | cdc6 normal growthcdc1-18, cdc6-OP, agar, thiamine added
cdc1 | cdc6 normal growthcdc1-M78, cdc6-OP, agar, thiamine added
cdc1 | cdc6 inviable following spore germination, 1-3 cell divisions and elongatedcdc1-P13(D389G), cdc6-121(unknown), standard temp
cdc1 | cdc27 normal veg cell growthcdc1-OP, cdc27-P11(unknown), agar, standard temp
cdc1 | cdc27 inviable veg cellscdc1-delta, cdc27-OP
cdc1 | cdc27 slow cell growthcdc1-P13(D389G), cdc27-OP
cdc1 | cdc27 elongated cellscdc1-P13(D389G), cdc27-OP
cdc27 | rad1 inviable vegetative cells cellscdc27-P11(unknown), radl-1, high temp
cdc27 | rad3 inviable vegetative cells cellscdc27-P11(unknown), rad3-136, high temp
cdc27 | rad9 inviable vegetative cells cellscdc27-P11(unknown), rad9-192, high temp
cdc27 | rad17 inviable vegetative cells cellscdc27-P11(unknown), radl7-W, high temp
cdc27 | hus1 inviable vegetative cells cellscdc27-P11(unknown), husl-14, high temp
cdc27 | rad27 inviable vegetative cells cellscdc27-P11(unknown), rad27-delta, high temp
cdc27 | rad1 viable vegetative cells cellscdc27-P11(unknown), radl-1, standard temp
cdc27 | rad3 viable vegetative cells cellscdc27-P11(unknown), rad3-136, standard temp
cdc27 | rad9 viable vegetative cells cellscdc27-P11(unknown), rad9-192, standard temp
cdc27 | rad17 viable vegetative cells cellscdc27-P11(unknown), radl7-W, standard temp
cdc27 | hus1 viable vegetative cells cellscdc27-P11(unknown), husl-14, standard temp
cdc27 | rad27 viable vegetative cells cellscdc27-P11(unknown), rad27-delta, standard temp
cdc1 | rad1 inviable germinating spores, 2-5 cell divisionscdc1-P13(D389G), radl-1, standard temp
cdc1 | rad3 inviable germinating spores, 2-5 cell divisionscdc1-P13(D389G), rad3-136, standard temp
cdc1 | rad9 inviable germinating spores, 2-5 cell divisionscdc1-P13(D389G), rad9-192, standard temp
cdc1 | rad17 inviable germinating spores, 2-5 cell divisionscdc1-P13(D389G), radl7-W, standard temp
cdc1 | hus1 inviable germinating spores, 2-5 cell divisionscdc1-P13(D389G), husl-14, standard temp
cdc1 | rad27 decreased cell growthcdc1-P13(D389G), rad27-delta, standard temp
cdc1 | rad27 shorter than normal cellscdc1-P13(D389G), rad27-delta, standard temp
cdc1 | rad27 inviable germinating spores, 2-5 cell divisionscdc1-P13(D389G), rad27-delta, high temp


9197411http://curation.pombase.org/pombe/curs/f1d8eeb553561925
ade6 | ura4 | leu1sensitive to high pH (abolsihed growth)CGA, EMM, liquid, ade6-216, ura4-D18, leu1-32
pub1 | ade6 | ura4 | leu1sensitive to high pH (almost abolished)CGA, EMM, liquid, pub1-1, ade6-216, ura4-D18, leu1-32
pub1 | elp3 | ade6 | ura4 | leu1sensitive to high pH (decreased growth)CGA, EMM, liquid, pub1-1, elp3-1, ade6-216, ura4-D18, leu1-32
sod1 | ade6 | ura4 | leu1sensitive to lithium CGA, EMM, liquid, sod1-delta, ade6-216, ura4-D18, leu1-32
pub1 | ade6 | ura4 | leu1 sensitive to lithium CGA, deletion, EMM, liquid, pub1-1, ade6-216, ura4-D18, leu1-32
elp3 | ade6 | ura4 | leu1 normal growth on lithiumCGA, deletion, EMM, liquid, elp3-1, ade6-216, ura4-D18, leu1-32
sod2 | pub1 | ade6 | ura4 | leu1 sensitive to lithium CGA, deletion, EMM, liquid, sod2-delta, pub1-1, ade6-216, ura4-D18, leu1-32
sod2 | elp3 | ade6 | ura4 | leu1 sensitive to lithium CGA, deletion, EMM, liquid, sod2-delta, elp3-1, ade6-216, ura4-D18, leu1-32
elp3 | pub1normal growth at low pHmicro, elp3-1, pub1-1(OP), standard temp, emm, agar
elp3 | pub1sensitive to low pHmicro, elp3-1, pub1-1, standard temp, emm, agar
elp3 | pub1elongated vegetative cellsmicro, elp3-1, pub1-1, standard temp, emm


9203579http://curation.pombase.org/pombe/curs/583bc6e2762656b4
cdc7long multinucleate inviablecdc7-24,cdc7(K38R)OP, micro
cdc7 | spg1 normal morphologycdc7-24,cdc7(K38R)OP, spg1-OP, micro
cdc7 | spg1 normal growth at high tempcdc7-OP,spg1-B8, cga, high temp
cdc7 | spg1 decreased growth on glucosecdc7-OP,spg1-delta, cga
cdc7 | spg1 viable binucleate septate cellscdc7-OP,spg1-delta, cga, emm
cdc2| spg1 increased septation index cdc2-17, spg1-OP
cdc2| spg1 septated mononucleate cells cdc2-17, spg1-OP
cdc2| spg1 (septation in the absence of nuclear division)cdc2-17, spg1-OP
cdc10| spg1 septated mononucleate cellscdc10-V50, spg1-OP
cdc15| spg1 abolished septum formationcdc15-140, spg1-OP
cdc16| spg1 multiseptatecdc16-116, spg1-OP, micro, high temp
cdc11| spg1 multiseptatecdc11-136, spg1-OP
cdc7| spg1 abolished septum formationcdc7-delta, spg1-OP
cdc16| spg1 viablecdc16-116, spg1-B8, high temp
spg1 | cdc7 decreased growth cdc7-24, spg1-B8, agar, ye, low temp
spg1 | cdc7 inviable cdc7-24, spg1-B8, agar, ye, standard temp
spg1 | cdc7 inviable at high temp cdc7-24, spg1-B8, agar, ye, high temp
spg1 | cdc7 decreased growth cdc7-A20, spg1-B8, agar, ye, low temp
spg1 | cdc7 inviable cdc7-A20, spg1-B8, agar, ye, standard temp
spg1 | cdc7 inviable at high temp cdc7-A20, spg1-B8, agar, ye, high temp
spg1 | cdc11 decreased growth cdc11-136, spg1-B8, agar, ye, low temp
spg1 | cdc11 inviable cdc11-136, spg1-B8, agar, ye, standard temp
spg1 | cdc11 inviable at high temp cdc11-136, spg1-B8, agar, ye, high temp
spg1 | cdc14 decreased growth cdc14-118, spg1-B8, agar, ye, low temp
spg1 | cdc14 inviable cdc14-118, spg1-B8, agar, ye, standard temp
spg1 | cdc14 inviable at high temp cdc14-118, spg1-B8, agar, ye, high temp
spg1 | cdc15 decreased growth cdc15-140, spg1-B8, agar, ye, low temp
spg1 | cdc15 inviable cdc15-140, spg1-B8, agar, ye, standard temp
spg1 | cdc15 inviable at high temp cdc15-140, spg1-B8, agar, ye, high temp
spg1 | cdc15 decreased growth cdc16-116, spg1-B8, agar, ye, low temp
spg1 | cdc15 decreased growth cdc16-116, spg1-B8, agar, ye, standard temp
spg1 | cdc15 decreased growth at high temp cdc16-116, spg1-B8, agar, ye, high temp


2107403http://curation.pombase.org/pombe/curs/e80c868d6c0d1ca5
ste6 | ras1 normal formation of diploid zygotesste6-delta, ras1-val17
ste6 | ras1 shmoos with long tipsste6-delta, ras1-val17
ste6 | ras1 increased conjugation frequencyste6-delta, ras1-val17
ste6 | ras1 decreased mating efficiencyste6-delta, ras1-val17


1340462http://curation.pombase.org/pombe/curs/34d8f97a921167ba
cgs1 | gpa2 increased cellular cAMP levelannotation_extension=has_expressivity(FYPO_EXT:0000003) cgs2-delta, gpa2-delta
cgs1 | gpa2 increased cellular cAMP levelannotation_extension=has_expressivity(FYPO_EXT:0000001) cgs2-delta, gpa2-R176H
cgs1 | gpa2 increased cellular cAMP levelannotation_extension=has_expressivity(FYPO_EXT:0000001) cgs2-delta, gpa2-Q202L


1563349http://curation.pombase.org/pombe/curs/69403b8ceb819841
rad1 | cdc17 inviable at high temphigh temp, cga, rad1-1, cdc17-K42
rad1 | wee1 inviable at high temphigh temp, cga, rad1-1, wee1-50
rad1 | wee1 viable vegetative cellsstandard temp, cga, rad1-1, wee1-50
rad1 | wee1 multinucleatehigh temp, cga, rad1-1, wee1-50
rad1 | wee1 misshapen nucleihigh temp, cga, rad1-1, wee1-50


1594599http://curation.pombase.org/pombe/curs/38560610372534b4
rad3elongated vegetative cellsrad3-136, rad3-OP, micro
rad3normal growth on HUrad3-136, rad3-OP, CGA


1464319http://curation.pombase.org/pombe/curs/65c76fa511461156
pyp1 | pyp2 abolished spore germinationpyp1-delta, pyp2-delta, cga
pyp1 | cdc25 small viable vegetative cellspyp1-delta, cdc25-22, standard, micro
pyp2 | wee1 elongated viable cellspyp2-delta, wee1-50-OP, standard temp, micro
pyp1 | nim1 wee cellspyp1-delta, nim1-delta, standard temp, micro
pyp1 | cdc25 mitotic catastrophepyp1-delta, cdc25-OP, standard temp, micro
pyp1 | cdc25 viablepyp1-delta, cdc25-22, high temp, CGA, agar,
pyp2 | cdc25 viablepyp2-delta, cdc25-22, high temp, CGA, agar, yes
pyp2 | cdc25 really elongated cells (30 um so high expressivity)pyp2-OP, cdc25-22, standard temp, CGA, agar, yes


1756736http://curation.pombase.org/pombe/curs/1cf6a37d7872772f
wis1 | wee1 weewis1-delta, wee1-50, high temp, micro
wis1 | cdc2 normal lengthwis1-delta, cdc2-1w, micro
wis1 | cdc2 normal lengthwis1-delta, cdc2-3w, micro
wis1 | ppa1 elongated viable cellswis1-delta, ppa1-delta, micro
wis1 | ppa2 elongated viable cellswis1-delta, ppa2-delta, micro


1435723http://curation.pombase.org/pombe/curs/b5aa547b2e76978f
byr2 | mat1pmnormal meiosisste8-mutant, matipm-OP, micro, occurs_in(diploid)
byr2 | mat1pmnormal sporulationste8-mutant, matipm-OP, micro, occurs_in(diploid)


23115244http://curation.pombase.org/pombe/curs/1f78fe7de80af20c
fep1 | fxn1 (frataxin) increased RNA level during veg growthdeletion, northern, assayed_using(fio1), yes
fep1 | fxn1 increased RNA level during veg growthdeletion, northern, assayed_using(str3), yes
fep1 | fxn1 decreased RNA level during veg growthdeletion, northern, assayed_using(pcl1), yes
fep1 | fxn1 increased RNA level during iron ion starvationdeletion, northern, assayed_using(fio1), yes
fep1 | fxn1 increased RNA level during iron ion starvationdeletion, northern, assayed_using(str3), yes
fep1 | fxn1 increased RNA level during iron ion starvationdeletion, northern, assayed_using(pcl1), yes
fep1 | fxn1 decreased hypox cell growth on glucosedeletion, cga, yes, plates
fep1 | fxn1 inviable on aerobic glucosedeletion, cga, yes, plates
php4 | fxn1 decreased growth on aerobic glucosedeletion, cga, yes, plates
php4 | fxn1 decreased growth on aerobic glucosedeletion, cga, yes, liquid
php4 | fxn1 sensitive to iron ion starvationdeletion, cga, yes, plates
php4 | fxn1 increased RNA level during veg growthdeletion, northern, assayed_using(pcl1), yes
php4 | fxn1 increased RNA level during iron ion starvdeletion, northern, assayed_using(pcl1), yes
php4 | fxn1 increased RNA level during veg growthdeletion, northern, assayed_using(isa1), yes
php4 | fxn1 increased RNA level during iron ion starvdeletion, northern, assayed_using(isa1), yes
php4 | fxn1 increased RNA level during veg growthdeletion, northern, assayed_using(fio1), yes
php4 | fxn1 increased RNA level during iron ion starvdeletion, northern, assayed_using(fio1), yes
php4 | fxn1 increased RNA level during veg growthdeletion, northern, assayed_using(str3), yes
php4 | fxn1 increased RNA level during iron ion starvdeletion, northern, assayed_using(str3), yes
grx4 | fxn1 decreased RNA level during veg growthdeletion, northern, assayed_using(pcl1), yes
grx4 | fxn1 increased RNA level during veg growthdeletion, northern, assayed_using(fio1), yes


1855255http://curation.pombase.org/pombe/curs/e113c7320f53efc5
pim1 | cdc2cell cycle arrest in mitotic G2cdc2-33, pim1-46, micro
pim1 | cdc13cell cycle arrest in mitotic G2cdc13-117, pim1-46, micro
pim1 | spi1inviable sporepim1-delta, spi1-op, cga
pim1 | spi1viablepim1-46, spi1-op, cga


1846086http://curation.pombase.org/pombe/curs/c93df2f114eaee6e
sds22 | sds23viable vegetative cellssds22-delta, sds23-OP, emm
sds22 | sds21viable vegetative cellssds22-delta, sds21-OP, emm
sds22 | sds21 | dis2inviable sporesds22-OP, sds21-delta, dis2-delta
dis2 | sds21viable vegetative cellssds21-delta, dis2-OP,
dis2 | sds21viable vegetativesds21-OP, dis2-delta,
dis2 | sds21inviablesds21-delta, dis2-delta,
dis2 | sds22viable vegetative cellssds22-OP, dis2-cs,


3042386http://curation.pombase.org/pombe/curs/a64b97197e845b5b
ras1 | mat1-Mnormal sporulationcga, ras1-delta, mat1-M-OP
ras1 | mat1-Pnormal sporulationcga, ras1-delta, mat1-P-OP
ras1 | byr1normal sporulationmicro, diploid cells, ras1-delta, byr1-OP
ras1 | byr1abolished conjugationmicro, ras1-delta, byr1-OP
ras1 | byr1abnormal morphologymicro, ras1-delta, byr1-OP
ras1 | byr1elongated conjugation tubes (shmoo)micro, ras1-G17V, byr1-OP
ras1 | byr1sterilemicro, ras1-OP, byr1-delta


3034608http://curation.pombase.org/pombe/curs/93c0ecbe5a58d0fe
mei2 | mei3viable vegetative cellsmei3-OP, mei2.16, CGA
3357510http://curation.pombase.org/pombe/curs/b2b393b33cecb3fd
mei3| ran1 sporulation in haploidboth OP CGA


6090122http://curation.pombase.org/pombe/curs/b2369c57c9545f6d
top1 | end1 decreased DNA topoisomerase activitytop1-710, end1-458 enzyme assay
top1 | end1 decreased endodeoxyribonuclease activitytop1-710, end1-458 enzyme assay
top1 | end1 viable vegetative cellstop1-710, end1-458 cga
top1 | top2 abnormal chromatin organization during vegetative growthstrain 3421 unknown microscopy high temp
top1 | top2 inviable high tempstrain 3421 unknown CGA high temp


9135083http://curation.pombase.org/pombe/curs/6ff4c9edff12167b
wee1 | atf1 normal cell cycle arrest in mitotic G1 phase during nitrogen starvationwee1-50, gad7::ura4, flow cytometry
wee1 | atf1 decreased conjugationwee1-50, gad7::ura4, CGA
wee1 | atf1 decreased RNA level during nitrogen starvationwee1-50, gad7::ura4, transcript expr, annotation_extension=assayed_using(PomBase:SPBC32C12.02)
15347659http://curation.pombase.org/pombe/curs/4395e90f8fc7e6d8
cds1 | mad2 sensitive to HUboth deletion, cga yes 30 agar
cds1 | mad2 normal growth during UV radboth deletion, cga yes 30 agar
cds1 | mad2 sensitive to MMSboth deletion, cga yes 30