Changes between Version 15 and Version 16 of AntoniasDoubleMutants


Ignore:
Timestamp:
Oct 22, 2012, 10:42:26 AM (9 years ago)
Author:
antonialock
Comment:

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  • AntoniasDoubleMutants

    v15 v16  
    491491||spbc530.05| pap1||decreased RNA level during cellular response to purvalanol A||deletion, quant pcr, assayed using bfr1||
    492492
    493 
     493[[BR]]
     494||22840777||http://curation.pombase.org/pombe/curs/f94801dfb551d839||
     495||prt1delta|bfr1delta||sensitive to BFA||yes, agar, standard temp||
     496||prt1delta|bfr1delta||sensitive to cycloheximide||yes, agar, standard temp||
     497||prt1delta|bfr1delta||sensitive to purA||yes, agar, standard temp||
     498||bfr1 | SPAC17C9.16c| sensitive to BFA||deletion, agar, normal temp, yes||
     499||bfr1 | SPBC36.03c| sensitive to BFA||deletion, agar, normal temp, yes||
     500||bfr1 | SPAC17C9.16c| sensitive to cycloheximide||deletion, agar, normal temp, yes||
     501||bfr1 | SPBC36.03c| sensitive to cycloheximide||deletion, agar, normal temp, yes||
     502||bfr1 | SPAC17C9.16c| sensitive to purA||deletion, agar, normal temp, yes||
     503||bfr1 | SPBC36.03c| sensitive to purA||deletion, agar, normal temp, yes||
     504||caf5 | SPAC17C9.16c||sensitive to purvalanol A||deletion, yes, agar, standard temp||
     505||caf5 | SPAC17C9.16c||sensitive to cycloheximide||deletion, yes, agar, standard temp||
     506||caf5 | SPAC17C9.16c||sensitive to BFA||deletion, yes, agar, standard temp||
     507
     508||prt1delta| pap1delta | bfr1delta | pap1 overexpressed || resistance to BFA||CGA, EMM, agar, normal temp||
     509||prt1delta| pap1delta | bfr1delta | SPAC22A12.16 overexpressed || resistance to BFA||CGA, EMM, agar, normal temp||
     510||prt1delta| pap1delta | bfr1delta | SPAC17C9.16c overexpressed || resistance to BFA||CGA, EMM, agar, normal temp||
     511||prt1delta| pap1delta | bfr1delta | caf5 overexpressed || resistance to BFA||CGA, EMM, agar, normal temp||
     512||prt1delta| pap1delta | bfr1delta | tcg1 overexpressed || resistance to BFA||CGA, EMM, agar, normal temp||
     513||prt1delta| pap1delta | bfr1delta | glo3 overexpressed || resistance to BFA||CGA, EMM, agar, normal temp||
     514||prt1delta| pap1delta | bfr1delta | SPAC2212.06c overexpressed || resistance to BFA||CGA, EMM, agar, normal temp||
     515||prt1delta| pap1delta | bfr1delta | pmc2 overexpressed || resistance to BFA||CGA, EMM, agar, normal temp||
     516||prt1delta| pap1delta | bfr1delta | cct8 overexpressed || resistance to BFA||CGA, EMM, agar, normal temp||
     517||prt1delta| pap1delta | bfr1delta | SPAC12G12.07c overexpressed || resistance to BFA||CGA, EMM, agar, normal temp||
     518||prt1delta| pap1delta | bfr1delta | SPBC839.14c overexpressed || resistance to BFA||CGA, EMM, agar, normal temp||
     519||prt1delta| pap1delta | bfr1delta | tdh1 overexpressed || resistance to BFA||CGA, EMM, agar, normal temp||
     520||prt1delta| pap1delta | bfr1delta | cpc2 overexpressed || resistance to BFA||CGA, EMM, agar, normal temp||
     521
     522||bfr1 | pmd1 | SPAC17C9.16c | caf5|| normal cell growth||deletion, yes, standard temp, agar||
     523||bfr1 | pmd1 | SPAC17C9.16c | caf5|| sensitive to brefeldin A||deletion, yes, standard temp, agar||
     524||bfr1 | pmd1| SPAC17C9.16c | caf5|| sensitive to CHX||deletion, yes, standard temp, agar||
     525||bfr1 | pmd1| SPAC17C9.16c | caf5|| sensitive to pur A||deletion, yes, standard temp, agar||
     526
     527||prt1| pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| sensitive to brefeldin A||deletion, yes, standard temp, agar||
     528||prt1| pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5|| sensitive to CHX||deletion, yes, standard temp, agar||
     529||prt1| pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5|| sensitive to our A||deletion, yes, standard temp, agar||
     530
     531||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| sensitive to brefeldin A||allele=MDR-sup(deletion), yes, standard temp, agar||
     532||pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5|| sensitive to CHX||MDR-sup,deletion, yes, standard temp, agar||
     533||pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5|| sensitive to pur A||MDR-sup, deletion, yes, standard temp, agar||
     534||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| sensitive to brefeldin A||allele=MDR-sup(deletion), EMM, standard temp, liquid||
     535||pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5|| sensitive to CHX||MDR-sup,deletion, EMM, standard temp, liquid||
     536||pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5|| sensitive to pur A||MDR-sup, deletion, EMM, standard temp, liquid||
     537||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| sensitive to brefeldin A||allele=MDR-sup(deletion), EMM, standard temp, agar||
     538||pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5|| sensitive to CHX||MDR-sup,deletion, EMM, standard temp, agar||
     539||pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5|| sensitive to pur A||MDR-sup, deletion, EMM, standard temp, agar||
     540
     541||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| sensitive to latrunculin A (actin assembly inhibitor)||allele=MDR-sup(deletion), yes, standard temp, liquid||
     542||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| sensitive to reversine (cell cycle kinase inhibitor) ||allele=MDR-sup(deletion), yes, standard temp, liquid||
     543||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| sensitive to hesperadin (cell cycle kinase inhibitor)||allele=MDR-sup(deletion), yes, standard temp, liquid||
     544||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| normal cellular response to MBC (tubulin poison) ||allele=MDR-sup(deletion), yes, standard temp, liquid||
     545||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| normal cellular response to benomyl (tubulin poison)||allele=MDR-sup(deletion), yes, standard temp, liquid||
     546
     547||prt1| pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| sensitive to cold||deletion, yes, low temp, agar||
     548||prt1| pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5|| sensitive to heat ||deletion, yes, high temp, agar||
     549||prt1| pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5|| normal cell growth||deletion, yes, normal temp, agar||
     550
     551||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| normal growth||allele=MDR-sup(deletion), yes, standard temp, agar||
     552||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| normal growth at high temp||allele=MDR-sup(deletion), yes, high temp, agar||
     553||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| normal growth at low temp||allele=MDR-sup(deletion), yes, low temp, agar||
     554||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| normal growth||allele=MDR-sup(deletion), yes, standard temp, liquid||
     555||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| normal growth at high temp||allele=MDR-sup(deletion), yes, high temp, liquid||
     556||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| normal growth at low temp||allele=MDR-sup(deletion), yes, low temp, liquid||
     557||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| normal growth||allele=MDR-sup(deletion), EMM, standard temp, liquid||
     558
     559||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| normal sporulation||allele=MDR-sup(deletion), standard temp||
     560||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| normal cell cycle progressionn||allele=MDR-sup(deletion), standard temp||
     561
     562||bfr1 | pmd1 | SPAC17C9.16c | caf5|| sensitive to brefeldin A||deletion, yes, standard temp, liquid culture||
     563||bfr1 | pmd1| SPAC17C9.16c | caf5|| sensitive to CHX||deletion, yes, standard temp, liquid culture||
     564||bfr1 | pmd1| SPAC17C9.16c | caf5|| sensitive to pur A||deletion, yes, standard temp, liquid culture||
     565
     566||prt1| pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| sensitive to brefeldin A||deletion, yes, standard temp, liquid culture||
     567||prt1| pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5|| sensitive to CHX||deletion, yes, standard temp, liquid culture||
     568||prt1| pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5|| sensitive to our A||deletion, yes, standard temp, liquid culture||
     569
     570||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| sensitive to brefeldin A|| MDR-sup, deletion, yes, standard temp, liquid culture||
     571||pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5|| sensitive to CHX|| MDR-sup, deletion, yes, standard temp, liquid culture||
     572||pap1 | bfr1 | pmd1| SPAC17C9.16c | caf5|| sensitive to our A|| MDR-sup, deletion, yes, standard temp, liquid culture||
     573||caf5 | SPAC17C9.16c | pmd1 | bfr1|| sensitive to to brefeldin A||deletion, yes, standard temp, agar||
     574
     575These requests may change depending on the SF item outcome because it is a screen against many compounds:
     576||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| sensitive to protease inhibitors|| MDR-sup, deletion||
     577||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| sensitive to kinase inhibitors|| MDR-sup, deletion||
     578||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| sensitive to topoisomerase inhibitors|| MDR-sup, deletion||
     579||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| sensitive to capsazepine (a vanilloid receptor antagonist) inhibitors|| MDR-sup, deletion||
     580||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| sensitive to PPT (an estrogen receptor agonist) || MDR-sup, deletion||
     581||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| sensitive to GW7647 (a receptor agonist) || MDR-sup, deletion||
     582
     583||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| sensitive to velcade || MDR-sup, deletion, yes, liquid, standard temp||
     584||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| sensitive to nocodazole (an inhibitor of microtubule polymerisation) || MDR-sup, deletion, yes, liquid, standard temp||
     585
     586||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5|| cell cycle arrest in metaphase || MDR-sup, deletion, velcade added||
     587||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5||abolished mitotic spindle assembly || MDR-sup, deletion, velcade and nocodazole added||
     588||pap1 | bfr1 | pmd1 | SPAC17C9.16c | caf5||clustered spindle pole bodies || MDR-sup, deletion, velcade and nocodazole added||
     589
     590
     591