Changes between Version 1 and Version 2 of AntoniasDoubleMutants


Ignore:
Timestamp:
Sep 13, 2012, 8:43:26 AM (9 years ago)
Author:
antonialock
Comment:

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  • AntoniasDoubleMutants

    v1 v2  
    1414||17369611||Antonia||
    1515||rtt109||Delta rtt109/Histone H3 K56R double-mutant phenotype||asynthetic sensitivity to DNA damage||
     16
     17[[BR]]
     18||18158900||Antonia||
     19||double mutant||sim3 mutants (allele=143(G81E),allele=205(E207K)) + overexpressed cnp1 = normal phenotype||||
     20||double mutant||sim3 mutants (allele=143(G81E),allele=205(E207K)) + overexpressed H4 = normal phenotype||||
     21||double mutant||sim3 mutants (allele=143(G81E),allele=205(E207K)) + overexpressed H3 = reduced viability (inviable ok)||||
     22
     23[[BR]]
     24
     25||cnb1,ppb1||dbl deletion mutant same phenotype as each single mutant (branched,elongated multi-septate cells + slow growth in presence of mgcl2||worth annotating? i.e. shows may be linked perhaps? dbl mutant not more severe||
     26||cnb1,ppb1,pmp1||dbl del + overexpressed = normal cell growth||||
     27||cnb1,ppb1||deletion,overexpr. slow growth in presence of mgcl2||i.e. large amounts of catalytic domain is not functional without regulatory subunit. is this interesting to capture?||
     28||cnb1,ppb1∆C(L445->STOP||deletion,overexpressed. slow growth in presence of mgcl2||constitutively active mutant is not active without regulatory subunit||
     29||cnb1,ppb1∆C(L445->STOP||overexpressed,overexpressed. phenotype growth arrest, round small bent pear-shaped cells. depolarized distr of cortical F-actin patches||normal phenotype in presence of CHEBI:61049||
     30||cnb1,ppb1∆C(L445->STOP,prz1||overexpressed,overexpressed, deleted. no growth arrest||||
     31
     32[[BR]]
     33||20018864||Antonia||
     34||deletion(prz1), overexpression(ncs1)||slow growth||cell growth assay||
     35||deletion(ncs1), overexpression(prz1)||slow growth||cell growth assay||
     36||deletion(prz1), overexpression(ncs1)||inviable in_response_to(CHEBI:29108)||IMP||
     37||deletion(ncs1), overexpression(prz1)||inviable in_response_to(CHEBI:29108)||IMP||
     38||deletion(ncs1),deletion(yam8)||normal cell growth in_presence_of(CHEBI:33120||cell growth assay||
     39
     40[[BR]]
     41||19606215||Antonia||
     42||cnx1||dbl mutant phenotype: abnormal cell wall morphology||allele=lumenal_cnx1p(aa_del488-560),allele=C-termTM_Cnx1p_cmyc(aa_del1-415)||
     43||check alleles||
     44
     45
     46[[BR]]
     47||18621924||Antonia||
     48||cyr1,cgs1 dbl deletion||nuclear export abolished||allele=deletion,annotation_extension=localizes(GeneDBSpombe:SPBC106.10)||
     49
     50
     51[[BR]]
     52||map3/mam2||diploid strain (CL:0000415) cannot sporulate if both receptors expressed at the same time||||
     53
     54[[BR]]
     55||1563351||Antonia||
     56||pat1/ras1||mat1-Pm transcribed||allele=pat1-114/deletion, at high temp||
     57||pat1/ste6||mat1-Pm transcribed||allele=pat1-114/deletion, at high temp||
     58
     59[BR]
     60||8196631||Antonia||
     61||mfm1/mfm2||FYPO:0000584 decreased sporulation||allele=deletion (both)||
     62||mfm1/mfm3||FYPO:0000584 decreased sporulation||allele=deletion (both)||
     63||mfm2/mfm3||FYPO:0000584 decreased sporulation||allele=deletion (both)||
     64||mfm1/mfm2/mfm3||FYPO:0000583 sporulation abolished||allele=deletion (all 3)||
     65||mfm1/mfm2/mfm3||FYPO:0000583 sporulation abolished||allele=deletion (all 3),annotation_extension=in_presence_of(M-factor - externally added),annotation_extension=occurs_in:CL0002674||
     66||mfm1/mfm2/mfm3||FYPO:0000590 normal sporulation||allele=deletion (all 3),annotation_extension=occurs_in:CL0002674||
     67
     68
     69[[BR]]
     70||8314086||Antonia||
     71||cyr1/sxa2||phenotype G1 arrest in presence of P-factor in rich media||allele=deletion||
     72||cyr1/sxa2||abnormal/increased shmoo formation in presence of P-factor in rich media||allele=deletion||
     73
     74[[BR]]
     75||18059475||Antonia||
     76||fkh2/fhl1||reduced zygote formation||allele=deletion||
     77||fkh2/mei4||reduced zygote formation||allele=deletion||
     78||fkh2/fhl1/mei4||reduced zygote formation||allele=deletion||
     79||cig2/fkh2||increased zygote formation||allele=deletion,happens_during(GO:0006995 - cellular response to nitrogen starvation)||
     80||cig2/fkh2/fhl1||reduced zygote formation||allele=deletion,happens_during(GO:0006995 - cellular response to nitrogen starvation)||
     81||cig2/fkh2/mei4||reduced zygote formation||allele=deletion,happens_during(GO:0006995 - cellular response to nitrogen starvation)||
     82||cig2/fkh2/fhl1/mei4||reduced zygote formation||allele=deletion,happens_during(GO:0006995 - cellular response to nitrogen starvation)||
     83
     84[[BR]]
     85||21118960||
     86||srw1 ectopically expressed | reb1 deletion||normal cell cycle arrest in response to pheromone||phenotype||
     87||reb1|wee1||phenotype: slow growth at high temperature||allele=deletion(reb1)/wee1-50||
     88||reb1|wee1||phenotype: multiseptate (0000118)||allele=deletion(reb1)/wee1-50||
     89||reb1|wee1||phenotype: wee||allele=deletion(reb1)/wee1-50||
     90||reb1|wee1||phenotype: spheroid cells||allele=deletion(reb1)/wee1-50||
     91||reb1|wee1||phenotype: DNA content increased||allele=deletion(reb1)/wee1-50||
     92||reb1|wee1||phenotype: normal cell growth at high temperature||in_presence_of:CHEBI:44423, allele=deletion(reb1)/wee1-50||     
     93||reb1|cdc10||phenotype: slow growth at high temperature||allele=deletion(reb1)/cdc10-129||
     94||reb1|cdc10||phenotype: abnormal? cell cycle arrest||qualifier=at_high_temperature,allele=deletion(reb1)/cdc10-129||
     95
     96[[BR]]
     97||9427748||
     98||deletion ppb1|overexpression pmp1||suppresses salt stress sensitivity||
     99||deletion pmp1|expression of const. active ppb1deltaC (C-term truncated)||suppresses salt stress sensitivity||
     100||deletion pmp1|expression of const. active ppb1deltaC (C-term truncated)||suppresses FK506 sensitivity||
     101||deletion pmp1|expression of ppb1 overexpressed||sensitive to salt stress and FK506||
     102||deletion pmp1|deletion ppb1|deletion pmk1||normal cell growth||
     103||deletion ppb1|deletionpmk1||filamentous and multiseptate||allele=deletion||
     104||deletion ppb1|overexpression pmp1||normal morphology||
     105
     106[[BR]]
     107||11461899||
     108||stm1|ras1||absence of G1 arrest in response to nitrogen starvation||allele+deletion (both) flow cytometry||
     109||stm1|ras1||slow cell growth in minimal media containing ammonia nitrogen source||allele=deletion (both) cell growth assay||
     110||stm1|gpa2||normal cell growth in minimal media containing ammonia nitrogen source||annotation_extension=condition(overexpression - stm1)allele=deletion (gpa2) cell growth assay||
     111||stm1|gpa2||normal septation||annotation_extension=condition(overexpression - stm1)allele=deletion (gpa2) cell growth assay||
     112
     113[[BR]]
     114||1448087||
     115||pyp1|pyp2||inviable germinating spore||allele=deletion (both)||
     116||pyp1 cdc25||cell cycle arrest||annotation_extension=condition(overexpression, pyp1), allele=cdc25-22, anotation_extension=condition(at_normal_temperature)|| 
     117||pyp2 cdc25||cell cycle arrest||annotation_extension=condition(overexpression, pyp1), allele=cdc25-22, anotation_extension=condition(at_normal_temperature)||
     118||pyp1 cdc25||cell cycle arrest||annotation_extension=condition(overexpression, pyp1), allele=cdc25-22, anotation_extension=condition(at_high_temperature)||
     119||pyp2 cdc25||cell cycle arrest||annotation_extension=condition(overexpression, pyp1), allele=cdc25-22, anotation_extension=condition(at_high_temperature)||
     120
     121||pyp1 cdc2||normal cell length (cell division/cell cycle)||annotation_extension=condition(overexpression, pyp1), allele=cdc2-1w||     
     122||pyp2 cdc2||normal cell length (cell division/cell cycle)||annotation_extension=condition(overexpression, pyp1), allele=cdc2-1w||
     123||pyp1 wee1||elongated cells||annotation_extension=condition(overexpression, pyp1), allele=wee1-50, anotation_extension=condition(at_normal_temperature)||     
     124||pyp2 wee1||elongated cells||annotation_extension=condition(overexpression, pyp1), allele=wee1-50, anotation_extension=condition(at_normal_temperature)||
     125||pyp1 wee1||normal cell length (possibly could use normal cell growth or normal cell division/mitotic cell cycle||annotation_extension=condition(overexpression, pyp1), allele=wee1-50, anotation_extension=condition(at_high_temperature)||
     126||pyp2 wee1||normal cell length (possibly could use normal cell growth or normal cell division/mitotic cell cycle||annotation_extension=condition(overexpression, pyp1), allele=wee1-50, anotation_extension=condition(at_high_temperature)||
     127
     128||pyp1 cdc25||normal cell length||allele=deletion, pyp1), allele=cdc25-22, annotation_extension=condition(at_normal_temperature)||     
     129||pyp2 cdc25||elongated cells||allele=deletion, pyp1), allele=cdc25-22, annotation_extension=condition(at_normal_temperature)||
     130||pyp1 cdc25||elongated cells||allele=deletion, pyp1), allele=cdc25-22, annotation_extension=condition(at_high_temperature)||
     131
     132[[BR]]
     133||15075260||
     134||pdf1||dbl mutant normal growth in presence of sodium orthovanadate||allele=S106A(S106A),allele=H478A(H478A) (2 plasmids)||
     135||pdf1||dbl mutant normal growth in presence of sodium orthovanadate||allele=D226A(D226A),allele=H478A(H478A) (2 plasmids)||
     136||pdf1||dbl mutant normal growth in presence of 'near alkaline/high' pH (=condition?)||allele=S106A(S106A),allele=H478A(H478A) (2 plasmids)||
     137||pdf1||dbl mutant normal growth in presence of 'near alkaline/high' pH (=condition?)||allele=D226A(D226A),allele=H478A(H478A) (2 plasmids)||
     138
     139[[BR]]
     140||9017391||http://curation.pombase.org/pombe/curs/40aa30b33b608f68||
     141||gcs1 + ade6||sensitive to cadmium||allele=apd1-1(unknown),allele=ade6-210(unknown)||
     142||gcs1 + ade6||sensitive to cadmium||allele=deletion,allele=ade6-210(unknown))||
     143||gcs1 + ade6||normal growth in presence of either ions: Ca|Na|Mg||allele=apd1-1(unknown),allele=ade6-210(unknown)||
     144||gcs1 + ade6||normal cell growth||annotation_extension=condition(in_rich_media),allele=apd1-1(unknown),allele=ade6-210(unknown)||
     145||gcs1 + ade6||resistant to MNNG (N-methyl-N'-nitro-N-nitrosoguanidine)||allele=deletion,allele=ade6-210(unknown))|
     146||gcs1 + ade6||slow cell growth||annotation_extension=condition(in_minimal_media),allele=apd1-1(unknown),allele=ade6-210(unknown)||
     147||gcs1 + ade6||slow cell growth||annotation_extension=condition(in_minimal_media),allele=deletion,allele=ade6-210(unknown)||
     148||gsh2 + ade6||slow cell growth||annotation_extension=condition(in_minimal_media),allele=deletion,allele=ade6-210(unknown)||
     149||hmt1 + ade6||normal cell growth||annotation_extension=condition(in_minimal_media),allele=deletion,allele=ade6-210(unknown)||
     150||gcs1 + ade6||normal cell growth||annotation_extension=condition(in_minimal_media),annotation_extension=in_presence_of(CHEBI:16856),allele=apd1-1(unknown),allele=ade6-210(unknown)||
     151||gcs1 + ade6||normal cell growth||annotation_extension=condition(in_minimal_media),annotation_extension=in_presence_of(CHEBI:16856),allele=deletion,allele=ade6-210(unknown)||
     152||gsh2 + ade6||normal cell growth||annotation_extension=condition(in_minimal_media),annotation_extension=in_presence_of(CHEBI:16856),allele=deletion,allele=ade6-210(unknown)||
     153||gcs1 + ade6||sporulation abolished||allele=apd1-1(unknown),allele=ade6-210(unknown)||
     154||gcs1 + ade6||normal sporulation||annotation_extension=in_presence_of(CHEBI:16856),allele=apd1-1(unknown),allele=ade6-210(unknown)||
     155||hmt1 + ade6||red pigmentation of colonies||allele=deletion,allele=ade6-210(unknown)||
     156||gcs1 + ade6||absent/reduced red pigmentation of colonies||allele=apd1-1(unknown),allele=ade6-210(unknown)||
     157||gcs1 + ade6||absent/reduced red pigmentation of colonies||allele=deletion,allele=ade6-210(unknown)||
     158||gsh2 + ade6||reduced red pigmentation of colonies||allele=deletion,allele=ade6-210(unknown)||
     159||gcs1 + ade6||absent/reduced red pigmentation of colonies||annotation_extension=condition(in_minimal_media),annotation_extension=in_presence_of(CHEBI:16856),allele=apd1-1(unknown),allele=ade6-210(unknown)||
     160
     161[[BR]]
     162||1448066||
     163||ade2/ade6||dbl mutant sensitive to cadmium||allele=ade6-216(unknown),allele=ade2(deletion), growth assay||
     164||ade2/ade7||dbl mutant sensitive to cadmium||allele=ade7-50(unknown),allele=ade2(deletion), growth assay||
     165||ade2/ade6||dbl mutant can't accumulate HMW PC-Cd-S2- complex||allele=ade6-216(unknown),allele=ade2(deletion)||
     166||ade2/ade7||dbl mutant can't accumulate HMW PC-Cd-S2- complex||allele=ade7-50(unknown),allele=ade2(deletion)||
     167
     168[[BR]]
     169||9016341||
     170||ade2 + ade7||sensitive to cisplatin||allele=deletion (ade2) allele=ade7.50||
     171||ade2 + ade6||sensitive to cisplatin||allele=deletion (ade2) allele=ade6.216||
     172
     173[[BR]]
     174||19646873||
     175||gef1delta + orb6-25|| normal morphology at high temp||microscopy||
     176||rga4 overexpressed + orb6-25|| normal morphology at high temp||microscopy||
     177||Gef1 overexpression + orb6-as2||stubby PCO:0000005 at normal temp||
     178||orb6-25 + rga4delta||stubby at normal temp PCO:0000005||
     179[[BR]]
     180||20396879||
     181||dak1/dak2 dbl mut||normal cell growth||allele=dak1-delta(deletion),allele=dak2-delta(deletion),condition=PCO:0000014||
     182||dak1/dak2||decreased glycerol dehydrogenase activity||allele=dak1-delta(deletion),allele=dak2-delta(deletion),condition=PCO:0000072||
     183||dak1/dak2||increased accumulation of DHA||allele=dak1-delta(deletion),allele=dak2-delta(deletion),condition=PCO:0000072||
     184||dak1/dak2||slow aerobic cell growth||allele=dak1-delta(deletion),allele=ak2-delta(deletion),condition=PCO:0000072||
     185||dak1/dak2/gld1||abolished glycerol dehydrogenase activity||allele=dak1-delta(deletion),allele=dak2-delta(deletion),allele=gld1-delta(deletion),condition=PCO:0000072||
     186||dak1/dak2||slow aerobic cell growth||allele=dak1-delta(deletion),allele=ak2-delta(deletion),condition=PCO:0000073||
     187||dak1/dak2||slow aerobic cell growth||allele=dak1-delta(deletion),allele=ak2-delta(deletion),condition=PCO:0000076,condition=PCO:0000014||
     188||tup11/tup12||increased glycerol dehydrogenase activity||allele=tup11-delta(deletion),allele=tup12-delta(deletion),condition=PCO:0000014||
     189||gld1/dak1/dak2||slow cell growth||allele=noname(overexpression),condition=PCO:0000077||
     190
     191
     192[[BR]]
     193||9585505||
     194||atf1 + pap1||sensitive to cisplatin||allele=atf1-delta(deletion), allele=pap1-delta(deletion), condition=PCO:0000012, CGA||
     195
     196[[BR]]
     197||1448080||
     198||crm1 + pap1||normal growth at cold /or/ normal cell growth condition=low temp||allele=crm1-809(unknown),allele=pap1-delta(deletion),PCO:0000012||
     199||crm1 + pap1||sensitive to staurosporine||allele=crm1-809(unknown),allele=pap1-delta(deletion),PCO:0000012||
     200||crm1 + pap1||normal protein accumulation||allele=crm1-809(unknown),allele=pap1-delta(deletion),annotation_extension=has_regulation_target(PomBase:SPAC3C7.14c)||
     201
     202
     203[[BR]]
     204||7642144||
     205||rpn11| pmd1||resistant to K-252a||allele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012||
     206||rpn11| pmd1||resistant to staurosporine||allele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012||
     207||rpn11| pmd1||resistant to vanadate||allele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012||
     208||rpn11| pmd1||resistant to cycloheximide||allele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012||
     209||rpn11| pmd1||resistant to actinomycin D||qualifier=NOT, allele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012||
     210||rpn11| pmd1||resistant to thiabendazole||qualifier=NOT, allele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012||
     211
     212[[BR]]
     213||21324894||
     214||cia1 | mts2||increased protein level|| allele=asf1-30(F24S,​G120S,​D173G), allele=mts2-1(unknown), condition=PCO:0000004,annotation_extension=has_regulation/downstream_target(PomBase:SPCC663.05c), western||
     215||cia1 | mts2||slow growth|| allele=asf1-30(F24S,​G120S,​D173G), allele=mts2-1(unknown), condition=PCO:0000005, condition=PCO:0000012, CGA||
     216||cia1 | mts2||slow growth at high temp|| allele=asf1-30(F24S,​G120S,​D173G), allele=mts2-1(unknown), condition=PCO:0000012, CGA||
     217||cia1 | ubc4||slow growth|| allele=asf1-30(F24S,​G120S,​D173G), allele=ubc4ts(P61S), condition=PCO:0000005, condition=PCO:0000012, CGA||
     218||cia1 | ubc4||slow growth at high temp|| allele=asf1-30(F24S,​G120S,​D173G), allele=ubc4ts(P61S), condition=PCO:0000012, CGA||
     219
     220||cia1 + mts2||FYPO:0000783, cia1 normally found in nucleus, but here found in cytoplasm||allele=asf1-30(F24S,​G120S,​D173G),allele=mts2-1(unknown), condition=PCO:0000004, specify cia in annex? has_target cia1?||
     221
     222||cia1 | ubc4||increased protein level|| allele=asf1-30(F24S,​G120S,​D173G), allele=ubc4ts(P61S), condition=PCO:0000004,annotation_extension=has_regulation/downstream_target(PomBase:SPCC663.05c), western||
     223||cia1 | SPBC2A(.04C||increased protein level|| allele=asf1-30(F24S,​G120S,​D173G), allele=san1-delta(deletion), condition=PCO:0000004,annotation_extension=has_regulation/downstream_target(PomBase:SPCC663.05c), western||
     224
     225||cia1 | hrd1 || slow growth at normal temp ||allele=asf1-30(F24S,​G120S,​D173G),allele=hrd1-delta(deletion), condition=PCO:0000012||
     226||cia1 | hrd1 || slow growth at high temp ||allele=asf1-30(F24S,​G120S,​D173G),allele=hrd1-delta(deletion), condition=PCO:0000012||
     227||cia1 | doa10 || slow growth at normal temp ||allele=asf1-30(F24S,​G120S,​D173G),allele=doa10-delta(deletion), condition=PCO:0000012||
     228||cia1 | doa10 || slow growth at high temp ||allele=asf1-30(F24S,​G120S,​D173G),allele=doa10-delta(deletion), condition=PCO:0000012||
     229
     230||cia1 | san1 || normal cell growth ||allele=asf1-30(F24S,​G120S,​D173G),allele=san1-delta(deletion), condition=PCO:0000012, condition=PCO:0000006||
     231||cia1 | san1 || slow growth at high temp ||allele=asf1-30(F24S,​G120S,​D173G),allele=san1-delta(deletion), condition=PCO:0000012||
     232||cia1 | ubr1 || normal cell growth ||allele=asf1-30(F24S,​G120S,​D173G),allele=ubr1-delta(deletion), condition=PCO:0000012, condition=PCO:0000006||
     233||cia1 | ubr1 || slow growth at high temp ||allele=asf1-30(F24S,​G120S,​D173G),allele=ubr1-delta(deletion), condition=PCO:0000012||
     234||cia1 | ubr11 || normal cell growth ||allele=asf1-30(F24S,​G120S,​D173G),allele=ubr11-delta(deletion), condition=PCO:0000012, condition=PCO:0000006||
     235||cia1 | ubr11 || slow growth at high temp ||allele=asf1-30(F24S,​G120S,​D173G),allele=ubr11-delta(deletion), condition=PCO:0000012||
     236||SPBC2A9.04c  | mis12 || normal cell growth at high temp||allele=san1-delta(deletion),allele=mis12-537(unknown), condition=PCO:0000012, condition=PCO:0000012||
     237||SPBC2A9.04c  | pim1 || normal cell growth at high temp||allele=san1-delta(deletion),allele=pim1-46(unknown), condition=PCO:0000012, condition=PCO:0000012||
     238||SPBC2A9.04c  | cnp1 || normal cell growth at high temp||allele=san1-delta(deletion),allele=cnp1-1(unknown), condition=PCO:0000012, condition=PCO:0000012||
     239
     240[[BR]]
     241||9135147||http://oliver0.sysbiol.cam.ac.uk/pombe/curs/67ba83cbaba024a7||
     242||pmk1 | spk1||sterile||allele=pmk1delta(deletion), allele=spk1delta(deletion)||
     243||pmk1 | spk1||abnormal morphology||allele=pmk1delta(deletion), allele=spk1delta(deletion), condition=PCO:0000014, condition=PCO:0000102||
     244||pmk1 | spk1||normal response to salt stress||allele=pmk1delta(deletion), allele=spk1delta(deletion), condition=PCO:0000014, condition=PCO:0000102||
     245||wis1 | pmk1 || multiseptate || microscopy, allele=wi1delta(deletion), allele=pmk1delta(deletion), condition=PCO:0000014, condition=PCO:0000102||
     246
     247[[BR]]
     248||8675019||http://oliver0.sysbiol.cam.ac.uk/pombe/curs/4b0970d0e5484486/||
     249||thi3 | bsu1 || slow aerobic cell growth || allele=bsu1delta(deletion), allele=thi3-1(unknown), condition=PCO:0000014, condition=PCO:0000106 ||
     250||thi3 | bsu1 || slow aerobic cell growth || allele=bsu1delta(deletion), allele=thi3-1(unknown), condition=PCO:0000014, condition=PCO:0000107 ||
     251
     252[[BR]]
     253||9560432||http://oliver0.sysbiol.cam.ac.uk/pombe/curs/de924eb687e556b7||
     254||itr2 | itr1 || normal cell growth || allele=IM49(unknown),​​ allele=itr1-noname(overexpression), condition=PCO:0000005,​​ condition=PCO:0000014,​​ condition=PCO:0000102,​ condition=PCO:0000111||
     255
     256[[BR]]
     257||22245228||http://curation.pombase.org/pombe/curs/7b18cacb1717e352||
     258||tpx1 | srx1 ||normal cellular response to hydrogen peroxide||allele=srx1(overexpression), allele=tpx1(deletion)||
     259
     260[[BR]]
     261||10455235||http://oliver0.sysbiol.cam.ac.uk/pombe/curs/32450f2b6ffd928f||
     262||gpx1 | ctt1|| normal response to hydrogen peroxide || ctt1=deleted, gpx1=overexpressed, 30 degrees||
     263||transcription factor deletions||reuest terms for during h202 and normal growth||see paper annotations||
     264
     265
     266[[BR]]
     267||8549830||http://curation.pombase.org/pombe/curs/78253143f2002b71||
     268||hxk1/hxk2||slow growth on glucose carbon source||double-deletion||
     269
     270[[BR]]
     271||2878925||http://curation.pombase.org/pombe/curs/462720c354cd7b67/||
     272||ade7-413|pma mutant || slow growth || adenine added, MM, liquid media||
     273
     274[[BR]]
     275||11136464||http://curation.pombase.org/pombe/curs/0089b9465b71c321/||
     276||inv1|sut1||slow growth on sucrose||allele=deletion(both), sucrose MM||
     277||inv1|sut1||slow growth on trehalose||allele=deletion(both), trehalose MM||
     278||inv1|sut1||normal growth on maltose||allele=deletion(both), maltose MM||
     279
     280[[BR]]
     281||9813085||http://curation.pombase.org/pombe/curs/b91556d0478e3dea||
     282||alg6|gpt1||decreased glc1man9GlcNAc level||DNJ added, chromatography, deletion of both||
     283||alg6|gpt1||increased man9GlcNAc level||DNJ addedchromatography, deletion of both||
     284||alg6|gpt1||absent glc2man9GlcNAc||DNJ added, chromatography, deletion of both||
     285||alg6|gpt1||absent glc3man9GlcNAc||DNJ added, chromatography, deletion of both||
     286||alg6|gpt1||decreased glc1man9GlcNAc level||chromatography, deletion of both||
     287||alg6|gpt1||decreased man9GlcNAc level||chromatography, deletion of both||
     288||alg6|gpt1||absent glc2man9GlcNAc||chromatography, deletion of both||
     289||alg6|gpt1||absent glc3man9GlcNAc||chromatography, deletion of both||
     290||gls2|alg6||absent glc2man9GlcNAc||chromatography, deletion of both||
     291||gls2|alg6||absent glc3man9GlcNAc||chromatography, deletion of both||
     292||gls2|alg6||increased glc1man9GlcNAc||chromatography, deletion of both||
     293||gls2|alg6||increased man9GlcNAc||chromatography, deletion of both||
     294||gls2|alg6||increased man8GlcNAc||chromatography, deletion of both||
     295||gpt1|alg6||increased RNA level||northern, annotation_extension=assayed_using(PomBase:SPAC22A12.15c) ||
     296||gpt1|alg6||increased RNA level||northern, annotation_extension=assayed_using(PomBase:SPAC22A12.15c) ||
     297||gpt1 | alg6||lower cell density in stationary phase||deletion, glucose minimal medium, liquid, normal temp||
     298||gpt1 | alg6||slower cell growth rate||deletion, glucose minimal medium, liquid, normal temp||
     299||gpt1 | alg6||flocculating cells, microscopy||deletion, glucose minimal medium, normal temp||
     300||gpt1 | alg6||round/swollen cells (they are not small), microscopy||deletion, glucose minimal medium, normal temp||
     301||gpt1 | alg6||slow growth at high temperature||deletion, glucose minimal medium, normal temp||
     302||gls2 | alg6||slow aerobic cell growth on glucose carbon source||normal glucose MM, high temp, agar plates||
     303||gls2 | alg6||normal aerobic cell growth on glucose carbon source||normal glucose MM, normal temp||
     304||gpt1 | alg6||slow aerobic cell growth on glucose carbon source||normal glucose MM, normal temp, agar plates||
     305||gpt1 | alg6||slow aerobic cell growth on glucose carbon source||normal glucose MM, high temp, agar plates||
     306||deletion alg6 | overexpressed gpt1||normal cell growth||plate, normal glucose MM, normal temp||
     307||deletion alg6 | overexpressed gpt1||normal cell growth||plate, normal glucose MM, high temp||
     308||gpt1|alg6||normal morphology||deletion, sorbitol added, normal glucose MM, liquid||
     309||gpt1|alg6||normal cell growth on gluocose carbon source||deletion, sorbitol added, normal glucose MM||
     310||deletion alg6 | overexpressed gpt1||normal morphology||normal glucose MM, high temp||
     311||deletion alg6| deletion gpt1|overexpression gpt1(Y1312A,D1352A)||round/swollen cells||normal temp, glucose MM||
     312||deletion alg6| deletion gpt1|overexpression gpt1(Y1312A,D1352A)||slow growth at high temp||high temp, glucose MM||
     313||gls2 | alg6||normal morphology||normal glucose MM, normal temp||
     314
     315[[BR]]
     316||11016847||http://curation.pombase.org/pombe/curs/373f4c6a1e37adf9||
     317||yam8 | pck2||normal growth at high temp||allele=ehs1-1, allele=pck2(overexpressed),high temp, YES||
     318||yam8 | pck2||slow growth at high temp||allele=yam8delta, allele=pck2(overexpressed),high temp, YES||
     319||yam8 | pck2||normal conjugation freq||allele=ehs1-1, allele=pck2(overexpressed)||
     320||yam8 | pck2||decreased conjugation freq||allele=yam8delta, allele=pck2(overexpressed)||
     321||yam8 | pck2||normal growth on cilofungin||allele=ehs1-1, allele=pck2(overexpressed),normal temp, YES||
     322||yam8 | pck2||sensitive to cilofungin||allele=yam8delta, allele=pck2(overexpressed),normal temp, YES||
     323||yam8 | pck2||normal growth on calcofluor white||allele=ehs1-1, allele=pck2(overexpressed),normal temp, YES||
     324||yam8 | pck2||sensitive to calcofluor white||allele=yam8delta, allele=pck2(overexpressed),normal temp, YES||
     325||yam8 | pck2||slow growth||allele=pck2(overexpression),allele=ehs1-1, solid agar plates, glucose minimal medium, standard temperature||
     326||yam8 | pck2||normal cell growth||allele=pck2(overexpression),allele=ehs1-1, calcium excluded, solid agar plates, glucose minimal medium, standard temperature||
     327||yam8 | pck2|| normal growth||allele=pck2(overexpression),allele=yam8delta, solid agar plates, glucose minimal medium, standard temperature||
     328||yam8 | pck2|| normal growth||allele=pck2(overexpression),allele=yam8delta, calcium excluded, solid agar plates, glucose minimal medium, standard temperature||
     329||yam8 | pck2||normal level of calcium in cell||allele=ehs1-1, allele=pck2(overexpression), normal temp, glucose MM||
     330||yam8 | pck2||inviable||allele=ehs1-1, allele=pck2delta||
     331||yam8 | pck2||viable||allele=yam8delta, allele=pck2delta||
     332||yam8 | pck2||normal morphology||allele=pck2(overexpression),allele=yam8delta, glucose MM, standard temp microscopy||
     333||yam8 | pck2||lysed cells||allele=pck2(overexpression),allele=ehs1-1, glucose MM, standard temp microscopy||
     334||yam8 | pck1||sensitive to calcofluor white||allele=ehs1-1, allele=pck1(overexpressed),normal temp, YES||
     335||yam8 | pck1||sensitive to cilofungin||allele=ehs1-1, allele=pck1(overexpressed),normal temp, YES||
     336||yam8 | pck1||slow growth at high temp||allele=ehs1-1, allele=pck1(overexpressed),high temp, YES||
     337
     338[[BR]]
     339||12896976||||
     340||pap1 | hba1 ||sensitive to caffeine ||pap1 deleted, hba1 overexpressed, standard glucose MM, standard temp, agar plates||
     341||pap1 | hba1 ||sensitive to caffeine ||pap1 deleted, hba1 deleted, standard glucose rich medium, standard temp, agar plates||
     342||sty1 | hba1 ||resistant to caffeine || sty1 deleted, hba1 deleted, standard glucose MM (yeah,diff from above), standard temp, agar plates||
     343
     344[[BR]]
     345||7565608||07cc7abfbaa6d1b0||
     346||rds1delta | ade6-D1||normal phenotype||
     347
     348[[BR]]
     349||18071249||http://curation.pombase.org/pombe/curs/5bff0cacfd0d068a||
     350||lcf1 | lcf2||decreased myristoyl-ligase activity||double deletion||
     351||lcf1 | lcf2||abolished palmitoyl-ligase activity||double deletion||
     352||lcf1 | lcf2||abolished oleoyl-ligase activity||double deletion||
     353||lcf1 | lcf2||decreased viability in stationary phase||double deletion, standard glucose MM||
     354||lcf1 | lcf2||decreased viability in stationary phase||double deletion, growth to stationary phase in rich medium followed by transfer to H2O||
     355||lcf1 | lcf2||decreased viability in stationary phase||double deletion, growth to stationary phase in rich medium followed by transfer to H2O||
     356||lcf1 | lcf2||decreased cell density in stationary phase||allele= double deletion, growth to stationary phase in rich medium followed by transfer to minimal medium||
     357
     358[[BR]]
     359||21811607||http://curation.pombase.org/pombe/curs/db6b03f9221f5e4a/||
     360||pkd2 | pmr1||normal immediate intracellular calcium spike following extracellular calcium stimulus||enzyme assay,allele=overexpression pkd2 / deletion(pmr1)||
     361||pkd2 | pmc1||normal immediate intracellular calcium spike following extracellular calcium stimulus||enzyme assay,allele=overexpression pkd2 / deletion(pmc1)||
     362||pkd2 | vcx1||normal immediate intracellular calcium spike following extracellular calcium stimulus||enzyme assay,allele=overexpression pkd2 / deletion(vcx1)||
     363||pek1 | cch1||normal calcium level in response to salt stress||pek1DD(unknown), overexpression, cch1delta, enzyme assay||
     364||pek1 | yam8||normal calcium level in response to salt stress||pek1DD(unknown), overexpression, yam8delta, enzyme assay||
     365||pek1 | yam8||normal calcium level in response to salt stress||pek1DD(unknown), overexpression, yam8delta, FK506 added, enzyme assay||
     366||1322 | ppb1||normal cellular response to salt stress||overexpression of 1322 and deletion of ppb1, cell growth assay, Na ions OR magnesium ions added||
     367||1322|prz1||decreased immediate intracellular calcium spike following extracellular calcium stimulus ||1322 overexpressed, prz1delta||
     368||1322|ppb1||absent immediate intracellular calcium spike following extracellular calcium stimulus ||1322 overexpressed, ppb1delta||
     369||pkd2|prz1||decreased immediate intracellular calcium spike following extracellular calcium stimulus ||pkd2 overexpressed, prz1delta||
     370||pkd2|prz1||decreased immediate intracellular calcium spike following extracellular calcium stimulus ||pkd2 overexpressed, ppb1delta||
     371||1322|pek1||normal cellular calcium level during salt stress||1322-delta, pek1DD(unknown)||
     372||1322|pek1||normal cellular calcium level during salt stress||1322-delta, pek1DD(unknown), tacrolimus added||
     373
     374[[BR]]
     375||9573052||
     376||pom1|cdc25||monopolar actin cortical patch localization ||allele=pom1delta, allele=cdc25-22, rhodamine-phalloidin added, high temp||
     377||pom1|cdc10||monopolar actin cortical patch localization to either end ||allele=pom1delta, allele=cdc10-V50, rhodamine-phalloidin added, high temp||
     378||pom1|cdc25||activation of monopolar cell growth at either end||allele=pom1delta, allele=cdc25-22, rhodamine-phalloidin added, high temp||
     379||pom1|cdc10||activation of monopolar cell growth at either end||allele=pom1delta, allele=cdc10-V50, rhodamine-phalloidin added, high temp||
     380||pom1|cdc11||branched cells||pom1delta,cdc11-136, high temp, calcofluor added||
     381||pom1|act1||slow growth||pom1delta, act1-48, at high, low and standard temp||
     382||pom1|cdc3||slow growth||pom1delta, cdc3-6, low temp, standard temp||
     383||pom1|cdc8||slow growth||pom1delta, cdc8-110, low temp, standard temp||
     384||pom1|nda3||slow growth||pom1delta, nda3-KM311, standard temp||
     385||pom1|ban5||slow growth||pom1delta, ban5-3, high temp, standard temp||
     386||pom1|cdc14||slow growth||pom1delta, cdc14-118||
     387||pom1|mid1||slow growth||pom1delta, dmf1-6||
     388||pom1|tea1||viable||pom1delta, tea1delta, high temp, standard temp, low temp||
     389||pom1|tea1||branched cells||pom1delta, tea1delta, high temp, standard temp, low temp||
     390||pom1|tea1||mislocalized septum||pom1delta, tea1delta||
     391
     392[[BR]]
     393||10224084||http://curation.pombase.org/pombe/curs/169c0f8873eee853||
     394||hmt2|sir1||normal cellular acid labile sulfide (S2-) level||hmt2JS563(E396K)|sir1delta, rich medium||
     395
     396[[BR]]
     397||10223994||http://curation.pombase.org/pombe/curs/b0116284c3e493a0||
     398||tps1|ntp1||resistant to freeze-thawing||allele=tps1(overexpressed),ntp1(deletion), EMM, solid agar, growth to exponential phase||
     399||tps1|ntp1||resistant to dehydration||allele=tps1(overexpressed),ntp1(deletion), EMM, solid agar, growth to exponential phase||
     400||tps1|ntp1||resistant to ethanol||allele=tps1(overexpressed),ntp1(deletion), EMM, solid agar, growth to exponential phase||
     401||tps1|ntp1||increased trehalose level during veg growth||allele=tps1(overexpressed),ntp1(deletion), MM, growth to exponential phase||
     402||tps1|ntp1||increased trehalose level in response to heat shock||allele=tps1(overexpressed),ntp1(deletion), MM, heat shock 30 mins 48 degrees, growth to exponential phase||
     403||tps1|ntp1||resistance to heat shock||CGA, allele=tps1(overexpressed),ntp1(deletion), MM, heat shock 30 mins 48 degrees, liquid culture, growth to exponential phase||
     404
     405[[BR]]
     406||9532803||http://curation.pombase.org/pombe/curs/a826f97af4e46f8f||
     407||pap1|rpn11||sensitive to brefeldin A||pap1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added||
     408||pap1|rpn11||normal response to staurosporine||pap1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added||
     409||pap1|rpn11||sensitive to K-252a||pap1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added||
     410||pap1|rpn11||sensitive to actinomycin D||pap1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added||
     411||pap1|rpn11||sensitive to cytochalasin B||pap1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added||
     412||pap1|bfr1||resistant to brefeldin A||pap1delta, bfr1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added||
     413||pap1|bfr1||resistant to staurosporine||pap1delta, bfr1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added||
     414||pap1|bfr1||resistant to K-252a||pap1delta, bfr1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added||
     415||pap1|bfr1||resistant to actinomycin D||pap1delta, bfr1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added||
     416||pap1|bfr1||resistant to cytochalasin B||pap1delta, bfr1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added||
     417||bfr1|rpn11||sensitive to brefeldin A||bfr1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added||
     418||bfr1|rpn11||resistant to staurosporine||bfr1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added||
     419||bfr1|rpn11||sensitive to K-252a||bfr1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added||
     420||bfr1|rpn11||sensitive to actinomycin D||bfr1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added||
     421||bfr1|rpn11||sensitive to cytochalasin B||bfr1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added||
     422||bfr1|pap1||sensitive to brefeldin A||bfr1delta, pap1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added||
     423||bfr1|pap1||resistant to staurosporine||bfr1delta, pap1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added||
     424||bfr1|pap1||sensitive to K-252a||bfr1delta, pap1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added||
     425||bfr1|pap1||sensitive to actinomycin D||bfr1delta, pap1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added||
     426||bfr1|pap1||normal cellular response to cytochalasin B||bfr1delta, pap1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added||
     427
     428[[BR]]
     429|16262791|||http://curation.pombase.org/pombe/curs/722def8c5161e3f1/|
     430||tsc2|rhb1||normal cellular response to canavanine||allele=tsc2delta,rhb1(D60K), CGA, agar plates, emm||
     431
     432
     433[[BR]]
     434||18637840||http://curation.pombase.org/pombe/curs/b719dc2cd003f460||
     435||zrt1 | zhf1 || sensitive to zinc and zinc starvation|| both deleted, liquid, standard temp||
     436||zrt1 | zhf1 || sensitive to zinc and zinc starvation|| both deleted, agar, standard temp, emm||
     437||zrt1 | zhf1||normal cellular copper level||spectroscopy, EMM, zinc added, both deleted||
     438||zrt1 | zhf1||normal cellular potassium level||spectroscopy, EMM, zinc added, both deleted||
     439||zrt1 | zhf1||normal cellular iron level||spectroscopy, EMM, zinc added, both deleted||
     440||zrt1 | zhf1||normal cellular manganese level||spectroscopy, EMM, zinc added, both deleted||
     441||zrt1 | zhf1||normal cellular magnesium level||spectroscopy, EMM, zinc added, both deleted||
     442||zrt1 | zhf1||normal cellular molybdenum level||spectroscopy, EMM, zinc added, both deleted||
     443||zrt1 | zhf1||normal cellular nickel level||spectroscopy, EMM, zinc added, both deleted||
     444||zrt1 | zhf1||normal cellular phosphorus level||spectroscopy, EMM, zinc added, both deleted||
     445||zrt1 | zhf1||normal cellular sulfur level||spectroscopy, EMM, zinc added, both deleted||
     446