wiki:AntoniasDoubleMutants

Version 15 (modified by antonialock, 9 years ago) (diff)

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https://sourceforge.net/apps/trac/pombase/wiki/AntoniasCuratedPapers


21745468Antonia
lkh1, dsk1((phenotype)) slow cell growth (cell growth assay)allele=Δdsk1Δlkh1
lkh1, dsk1((phenotype)) slow cell growth (cell growth assay)allele=Δdsk1,lkh1-K391A


7900424
mfm1/2/3triple deletion, sporulation abolisheddiploid
mfm1/2/3triple deletion, normal sporulationdiploid, M-factor added
mfm1/2/3triple deletion, normal sporulationdiploid, P-factor added
mfm1/2/3triple deletion, decreased protein levelhas regulation target(Pfactor), diploid


17369611Antonia
rtt109Delta rtt109/Histone H3 K56R double-mutant phenotypeasynthetic sensitivity to DNA damage


18158900Antonia
double mutantsim3 mutants (allele=143(G81E),allele=205(E207K)) + overexpressed cnp1 = normal phenotype
double mutantsim3 mutants (allele=143(G81E),allele=205(E207K)) + overexpressed H4 = normal phenotype
double mutantsim3 mutants (allele=143(G81E),allele=205(E207K)) + overexpressed H3 = reduced viability (inviable ok)


cnb1,ppb1dbl deletion mutant same phenotype as each single mutant (branched,elongated multi-septate cells + slow growth in presence of mgcl2worth annotating? i.e. shows may be linked perhaps? dbl mutant not more severe
cnb1,ppb1,pmp1dbl del + overexpressed = normal cell growth
cnb1,ppb1deletion,overexpr. slow growth in presence of mgcl2i.e. large amounts of catalytic domain is not functional without regulatory subunit. is this interesting to capture?
cnb1,ppb1∆C(L445->STOPdeletion,overexpressed. slow growth in presence of mgcl2constitutively active mutant is not active without regulatory subunit
cnb1,ppb1∆C(L445->STOPoverexpressed,overexpressed. phenotype growth arrest, round small bent pear-shaped cells. depolarized distr of cortical F-actin patchesnormal phenotype in presence of CHEBI:61049
cnb1,ppb1∆C(L445->STOP,prz1overexpressed,overexpressed, deleted. no growth arrest


20018864Antonia
deletion(prz1), overexpression(ncs1)slow growthcell growth assay
deletion(ncs1), overexpression(prz1)slow growthcell growth assay
deletion(prz1), overexpression(ncs1)inviable in_response_to(CHEBI:29108)IMP
deletion(ncs1), overexpression(prz1)inviable in_response_to(CHEBI:29108)IMP
deletion(ncs1),deletion(yam8)normal cell growth in_presence_of(CHEBI:33120cell growth assay


19606215Antonia
cnx1dbl mutant phenotype: abnormal cell wall morphologyallele=lumenal_cnx1p(aa_del488-560),allele=C-termTM_Cnx1p_cmyc(aa_del1-415)
check alleles


18621924Antonia
cyr1,cgs1 dbl deletionnuclear export abolishedallele=deletion,annotation_extension=localizes(GeneDBSpombe:SPBC106.10)


map3/mam2diploid strain (CL:0000415) cannot sporulate if both receptors expressed at the same time


1563351Antonia
pat1/ras1mat1-Pm transcribedallele=pat1-114/deletion, at high temp
pat1/ste6mat1-Pm transcribedallele=pat1-114/deletion, at high temp

[BR]

8196631Antonia
mfm1/mfm2FYPO:0000584 decreased sporulationallele=deletion (both)
mfm1/mfm3FYPO:0000584 decreased sporulationallele=deletion (both)
mfm2/mfm3FYPO:0000584 decreased sporulationallele=deletion (both)
mfm1/mfm2/mfm3FYPO:0000583 sporulation abolishedallele=deletion (all 3)
mfm1/mfm2/mfm3FYPO:0000583 sporulation abolishedallele=deletion (all 3),annotation_extension=in_presence_of(M-factor - externally added),annotation_extension=occurs_in:CL0002674
mfm1/mfm2/mfm3FYPO:0000590 normal sporulationallele=deletion (all 3),annotation_extension=occurs_in:CL0002674


8314086Antonia
cyr1/sxa2phenotype G1 arrest in presence of P-factor in rich mediaallele=deletion
cyr1/sxa2abnormal/increased shmoo formation in presence of P-factor in rich mediaallele=deletion


18059475Antonia
fkh2/fhl1reduced zygote formationallele=deletion
fkh2/mei4reduced zygote formationallele=deletion
fkh2/fhl1/mei4reduced zygote formationallele=deletion
cig2/fkh2increased zygote formationallele=deletion,happens_during(GO:0006995 - cellular response to nitrogen starvation)
cig2/fkh2/fhl1reduced zygote formationallele=deletion,happens_during(GO:0006995 - cellular response to nitrogen starvation)
cig2/fkh2/mei4reduced zygote formationallele=deletion,happens_during(GO:0006995 - cellular response to nitrogen starvation)
cig2/fkh2/fhl1/mei4reduced zygote formationallele=deletion,happens_during(GO:0006995 - cellular response to nitrogen starvation)


21118960
srw1 ectopically expressed | reb1 deletionnormal cell cycle arrest in response to pheromonephenotype
reb1|wee1phenotype: slow growth at high temperatureallele=deletion(reb1)/wee1-50
reb1|wee1phenotype: multiseptate (0000118)allele=deletion(reb1)/wee1-50
reb1|wee1phenotype: weeallele=deletion(reb1)/wee1-50
reb1|wee1phenotype: spheroid cellsallele=deletion(reb1)/wee1-50
reb1|wee1phenotype: DNA content increasedallele=deletion(reb1)/wee1-50
reb1|wee1phenotype: normal cell growth at high temperaturein_presence_of:CHEBI:44423, allele=deletion(reb1)/wee1-50
reb1|cdc10phenotype: slow growth at high temperatureallele=deletion(reb1)/cdc10-129
reb1|cdc10phenotype: abnormal? cell cycle arrestqualifier=at_high_temperature,allele=deletion(reb1)/cdc10-129


9427748
deletion ppb1|overexpression pmp1suppresses salt stress sensitivity
deletion pmp1|expression of const. active ppb1deltaC (C-term truncated)suppresses salt stress sensitivity
deletion pmp1|expression of const. active ppb1deltaC (C-term truncated)suppresses FK506 sensitivity
deletion pmp1|expression of ppb1 overexpressedsensitive to salt stress and FK506
deletion pmp1|deletion ppb1|deletion pmk1normal cell growth
deletion ppb1|deletionpmk1filamentous and multiseptateallele=deletion
deletion ppb1|overexpression pmp1normal morphology


11461899
stm1|ras1absence of G1 arrest in response to nitrogen starvationallele+deletion (both) flow cytometry
stm1|ras1slow cell growth in minimal media containing ammonia nitrogen sourceallele=deletion (both) cell growth assay
stm1|gpa2normal cell growth in minimal media containing ammonia nitrogen sourceannotation_extension=condition(overexpression - stm1)allele=deletion (gpa2) cell growth assay
stm1|gpa2normal septationannotation_extension=condition(overexpression - stm1)allele=deletion (gpa2) cell growth assay


1448087
pyp1|pyp2inviable germinating sporeallele=deletion (both)
pyp1 cdc25cell cycle arrestannotation_extension=condition(overexpression, pyp1), allele=cdc25-22, anotation_extension=condition(at_normal_temperature)
pyp2 cdc25cell cycle arrestannotation_extension=condition(overexpression, pyp1), allele=cdc25-22, anotation_extension=condition(at_normal_temperature)
pyp1 cdc25cell cycle arrestannotation_extension=condition(overexpression, pyp1), allele=cdc25-22, anotation_extension=condition(at_high_temperature)
pyp2 cdc25cell cycle arrestannotation_extension=condition(overexpression, pyp1), allele=cdc25-22, anotation_extension=condition(at_high_temperature)
pyp1 cdc2normal cell length (cell division/cell cycle)annotation_extension=condition(overexpression, pyp1), allele=cdc2-1w
pyp2 cdc2normal cell length (cell division/cell cycle)annotation_extension=condition(overexpression, pyp1), allele=cdc2-1w
pyp1 wee1elongated cellsannotation_extension=condition(overexpression, pyp1), allele=wee1-50, anotation_extension=condition(at_normal_temperature)
pyp2 wee1elongated cellsannotation_extension=condition(overexpression, pyp1), allele=wee1-50, anotation_extension=condition(at_normal_temperature)
pyp1 wee1normal cell length (possibly could use normal cell growth or normal cell division/mitotic cell cycleannotation_extension=condition(overexpression, pyp1), allele=wee1-50, anotation_extension=condition(at_high_temperature)
pyp2 wee1normal cell length (possibly could use normal cell growth or normal cell division/mitotic cell cycleannotation_extension=condition(overexpression, pyp1), allele=wee1-50, anotation_extension=condition(at_high_temperature)
pyp1 cdc25normal cell lengthallele=deletion, pyp1), allele=cdc25-22, annotation_extension=condition(at_normal_temperature)
pyp2 cdc25elongated cellsallele=deletion, pyp1), allele=cdc25-22, annotation_extension=condition(at_normal_temperature)
pyp1 cdc25elongated cellsallele=deletion, pyp1), allele=cdc25-22, annotation_extension=condition(at_high_temperature)


15075260
pdf1dbl mutant normal growth in presence of sodium orthovanadateallele=S106A(S106A),allele=H478A(H478A) (2 plasmids)
pdf1dbl mutant normal growth in presence of sodium orthovanadateallele=D226A(D226A),allele=H478A(H478A) (2 plasmids)
pdf1dbl mutant normal growth in presence of 'near alkaline/high' pH (=condition?)allele=S106A(S106A),allele=H478A(H478A) (2 plasmids)
pdf1dbl mutant normal growth in presence of 'near alkaline/high' pH (=condition?)allele=D226A(D226A),allele=H478A(H478A) (2 plasmids)


9017391http://curation.pombase.org/pombe/curs/40aa30b33b608f68
gcs1 + ade6sensitive to cadmiumallele=apd1-1(unknown),allele=ade6-210(unknown)
gcs1 + ade6sensitive to cadmiumallele=deletion,allele=ade6-210(unknown))
gcs1 + ade6normal growth in presence of either ions: Ca|Na|Mgallele=apd1-1(unknown),allele=ade6-210(unknown)
gcs1 + ade6normal cell growthannotation_extension=condition(in_rich_media),allele=apd1-1(unknown),allele=ade6-210(unknown)
gcs1 + ade6resistant to MNNG (N-methyl-N'-nitro-N-nitrosoguanidine)allele=deletion,allele=ade6-210(unknown))|
gcs1 + ade6slow cell growthannotation_extension=condition(in_minimal_media),allele=apd1-1(unknown),allele=ade6-210(unknown)
gcs1 + ade6slow cell growthannotation_extension=condition(in_minimal_media),allele=deletion,allele=ade6-210(unknown)
gsh2 + ade6slow cell growthannotation_extension=condition(in_minimal_media),allele=deletion,allele=ade6-210(unknown)
hmt1 + ade6normal cell growthannotation_extension=condition(in_minimal_media),allele=deletion,allele=ade6-210(unknown)
gcs1 + ade6normal cell growthannotation_extension=condition(in_minimal_media),annotation_extension=in_presence_of(CHEBI:16856),allele=apd1-1(unknown),allele=ade6-210(unknown)
gcs1 + ade6normal cell growthannotation_extension=condition(in_minimal_media),annotation_extension=in_presence_of(CHEBI:16856),allele=deletion,allele=ade6-210(unknown)
gsh2 + ade6normal cell growthannotation_extension=condition(in_minimal_media),annotation_extension=in_presence_of(CHEBI:16856),allele=deletion,allele=ade6-210(unknown)
gcs1 + ade6sporulation abolishedallele=apd1-1(unknown),allele=ade6-210(unknown)
gcs1 + ade6normal sporulationannotation_extension=in_presence_of(CHEBI:16856),allele=apd1-1(unknown),allele=ade6-210(unknown)
hmt1 + ade6red pigmentation of coloniesallele=deletion,allele=ade6-210(unknown)
gcs1 + ade6absent/reduced red pigmentation of coloniesallele=apd1-1(unknown),allele=ade6-210(unknown)
gcs1 + ade6absent/reduced red pigmentation of coloniesallele=deletion,allele=ade6-210(unknown)
gsh2 + ade6reduced red pigmentation of coloniesallele=deletion,allele=ade6-210(unknown)
gcs1 + ade6absent/reduced red pigmentation of coloniesannotation_extension=condition(in_minimal_media),annotation_extension=in_presence_of(CHEBI:16856),allele=apd1-1(unknown),allele=ade6-210(unknown)


1448066
ade2/ade6dbl mutant sensitive to cadmiumallele=ade6-216(unknown),allele=ade2(deletion), growth assay
ade2/ade7dbl mutant sensitive to cadmiumallele=ade7-50(unknown),allele=ade2(deletion), growth assay
ade2/ade6dbl mutant can't accumulate HMW PC-Cd-S2- complexallele=ade6-216(unknown),allele=ade2(deletion)
ade2/ade7dbl mutant can't accumulate HMW PC-Cd-S2- complexallele=ade7-50(unknown),allele=ade2(deletion)


9016341
ade2 + ade7sensitive to cisplatinallele=deletion (ade2) allele=ade7.50
ade2 + ade6sensitive to cisplatinallele=deletion (ade2) allele=ade6.216


19646873
gef1delta + orb6-25 normal morphology at high tempmicroscopy
rga4 overexpressed + orb6-25 normal morphology at high tempmicroscopy
Gef1 overexpression + orb6-as2stubby PCO:0000005 at normal temp
orb6-25 + rga4deltastubby at normal temp PCO:0000005


20396879
dak1/dak2 dbl mutnormal cell growthallele=dak1-delta(deletion),allele=dak2-delta(deletion),condition=PCO:0000014
dak1/dak2decreased glycerol dehydrogenase activityallele=dak1-delta(deletion),allele=dak2-delta(deletion),condition=PCO:0000072
dak1/dak2increased accumulation of DHAallele=dak1-delta(deletion),allele=dak2-delta(deletion),condition=PCO:0000072
dak1/dak2slow aerobic cell growthallele=dak1-delta(deletion),allele=ak2-delta(deletion),condition=PCO:0000072
dak1/dak2/gld1abolished glycerol dehydrogenase activityallele=dak1-delta(deletion),allele=dak2-delta(deletion),allele=gld1-delta(deletion),condition=PCO:0000072
dak1/dak2slow aerobic cell growthallele=dak1-delta(deletion),allele=ak2-delta(deletion),condition=PCO:0000073
dak1/dak2slow aerobic cell growthallele=dak1-delta(deletion),allele=ak2-delta(deletion),condition=PCO:0000076,condition=PCO:0000014
tup11/tup12increased glycerol dehydrogenase activityallele=tup11-delta(deletion),allele=tup12-delta(deletion),condition=PCO:0000014
gld1/dak1/dak2slow cell growthallele=noname(overexpression),condition=PCO:0000077


9585505
atf1 + pap1sensitive to cisplatinallele=atf1-delta(deletion), allele=pap1-delta(deletion), condition=PCO:0000012, CGA


1448080
crm1 + pap1normal growth at cold /or/ normal cell growth condition=low tempallele=crm1-809(unknown),allele=pap1-delta(deletion),PCO:0000012
crm1 + pap1sensitive to staurosporineallele=crm1-809(unknown),allele=pap1-delta(deletion),PCO:0000012
crm1 + pap1normal protein accumulationallele=crm1-809(unknown),allele=pap1-delta(deletion),annotation_extension=has_regulation_target(PomBase:SPAC3C7.14c)


7642144
rpn11| pmd1resistant to K-252aallele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012
rpn11| pmd1resistant to staurosporineallele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012
rpn11| pmd1resistant to vanadateallele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012
rpn11| pmd1resistant to cycloheximideallele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012
rpn11| pmd1resistant to actinomycin Dqualifier=NOT, allele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012
rpn11| pmd1resistant to thiabendazolequalifier=NOT, allele=pmd1-delta(deletion),allele=noname(overexpression),​condition=PCO:0000012


21324894
cia1 | mts2increased protein level allele=asf1-30(F24S,​G120S,​D173G), allele=mts2-1(unknown), condition=PCO:0000004,annotation_extension=has_regulation/downstream_target(PomBase:SPCC663.05c), western
cia1 | mts2slow growth allele=asf1-30(F24S,​G120S,​D173G), allele=mts2-1(unknown), condition=PCO:0000005, condition=PCO:0000012, CGA
cia1 | mts2slow growth at high temp allele=asf1-30(F24S,​G120S,​D173G), allele=mts2-1(unknown), condition=PCO:0000012, CGA
cia1 | ubc4slow growth allele=asf1-30(F24S,​G120S,​D173G), allele=ubc4ts(P61S), condition=PCO:0000005, condition=PCO:0000012, CGA
cia1 | ubc4slow growth at high temp allele=asf1-30(F24S,​G120S,​D173G), allele=ubc4ts(P61S), condition=PCO:0000012, CGA
cia1 + mts2FYPO:0000783, cia1 normally found in nucleus, but here found in cytoplasmallele=asf1-30(F24S,​G120S,​D173G),allele=mts2-1(unknown), condition=PCO:0000004, specify cia in annex? has_target cia1?
cia1 | ubc4increased protein level allele=asf1-30(F24S,​G120S,​D173G), allele=ubc4ts(P61S), condition=PCO:0000004,annotation_extension=has_regulation/downstream_target(PomBase:SPCC663.05c), western
cia1 | SPBC2A(.04Cincreased protein level allele=asf1-30(F24S,​G120S,​D173G), allele=san1-delta(deletion), condition=PCO:0000004,annotation_extension=has_regulation/downstream_target(PomBase:SPCC663.05c), western
cia1 | hrd1 slow growth at normal temp allele=asf1-30(F24S,​G120S,​D173G),allele=hrd1-delta(deletion), condition=PCO:0000012
cia1 | hrd1 slow growth at high temp allele=asf1-30(F24S,​G120S,​D173G),allele=hrd1-delta(deletion), condition=PCO:0000012
cia1 | doa10 slow growth at normal temp allele=asf1-30(F24S,​G120S,​D173G),allele=doa10-delta(deletion), condition=PCO:0000012
cia1 | doa10 slow growth at high temp allele=asf1-30(F24S,​G120S,​D173G),allele=doa10-delta(deletion), condition=PCO:0000012
cia1 | san1 normal cell growth allele=asf1-30(F24S,​G120S,​D173G),allele=san1-delta(deletion), condition=PCO:0000012, condition=PCO:0000006
cia1 | san1 slow growth at high temp allele=asf1-30(F24S,​G120S,​D173G),allele=san1-delta(deletion), condition=PCO:0000012
cia1 | ubr1 normal cell growth allele=asf1-30(F24S,​G120S,​D173G),allele=ubr1-delta(deletion), condition=PCO:0000012, condition=PCO:0000006
cia1 | ubr1 slow growth at high temp allele=asf1-30(F24S,​G120S,​D173G),allele=ubr1-delta(deletion), condition=PCO:0000012
cia1 | ubr11 normal cell growth allele=asf1-30(F24S,​G120S,​D173G),allele=ubr11-delta(deletion), condition=PCO:0000012, condition=PCO:0000006
cia1 | ubr11 slow growth at high temp allele=asf1-30(F24S,​G120S,​D173G),allele=ubr11-delta(deletion), condition=PCO:0000012
SPBC2A9.04c | mis12 normal cell growth at high tempallele=san1-delta(deletion),allele=mis12-537(unknown), condition=PCO:0000012, condition=PCO:0000012
SPBC2A9.04c | pim1 normal cell growth at high tempallele=san1-delta(deletion),allele=pim1-46(unknown), condition=PCO:0000012, condition=PCO:0000012
SPBC2A9.04c | cnp1 normal cell growth at high tempallele=san1-delta(deletion),allele=cnp1-1(unknown), condition=PCO:0000012, condition=PCO:0000012


9135147http://oliver0.sysbiol.cam.ac.uk/pombe/curs/67ba83cbaba024a7
pmk1 | spk1sterileallele=pmk1delta(deletion), allele=spk1delta(deletion)
pmk1 | spk1abnormal morphologyallele=pmk1delta(deletion), allele=spk1delta(deletion), condition=PCO:0000014, condition=PCO:0000102
pmk1 | spk1normal response to salt stressallele=pmk1delta(deletion), allele=spk1delta(deletion), condition=PCO:0000014, condition=PCO:0000102
wis1 | pmk1 multiseptate microscopy, allele=wi1delta(deletion), allele=pmk1delta(deletion), condition=PCO:0000014, condition=PCO:0000102


8675019http://oliver0.sysbiol.cam.ac.uk/pombe/curs/4b0970d0e5484486/
thi3 | bsu1 slow aerobic cell growth allele=bsu1delta(deletion), allele=thi3-1(unknown), condition=PCO:0000014, condition=PCO:0000106
thi3 | bsu1 slow aerobic cell growth allele=bsu1delta(deletion), allele=thi3-1(unknown), condition=PCO:0000014, condition=PCO:0000107


9560432http://oliver0.sysbiol.cam.ac.uk/pombe/curs/de924eb687e556b7
itr2 | itr1 normal cell growth allele=IM49(unknown),​​ allele=itr1-noname(overexpression), condition=PCO:0000005,​​ condition=PCO:0000014,​​ condition=PCO:0000102,​ condition=PCO:0000111


22245228http://curation.pombase.org/pombe/curs/7b18cacb1717e352
tpx1 | srx1 normal cellular response to hydrogen peroxideallele=srx1(overexpression), allele=tpx1(deletion)


10455235http://oliver0.sysbiol.cam.ac.uk/pombe/curs/32450f2b6ffd928f
gpx1 | ctt1 normal response to hydrogen peroxide ctt1=deleted, gpx1=overexpressed, 30 degrees
transcription factor deletionsreuest terms for during h202 and normal growthsee paper annotations


8549830http://curation.pombase.org/pombe/curs/78253143f2002b71
hxk1/hxk2slow growth on glucose carbon sourcedouble-deletion


2878925http://curation.pombase.org/pombe/curs/462720c354cd7b67/
ade7-413|pma mutant slow growth adenine added, MM, liquid media


11136464http://curation.pombase.org/pombe/curs/0089b9465b71c321/
inv1|sut1slow growth on sucroseallele=deletion(both), sucrose MM
inv1|sut1slow growth on trehaloseallele=deletion(both), trehalose MM
inv1|sut1normal growth on maltoseallele=deletion(both), maltose MM


9813085http://curation.pombase.org/pombe/curs/b91556d0478e3dea
alg6|gpt1decreased glc1man9GlcNAc levelDNJ added, chromatography, deletion of both
alg6|gpt1increased man9GlcNAc levelDNJ addedchromatography, deletion of both
alg6|gpt1absent glc2man9GlcNAcDNJ added, chromatography, deletion of both
alg6|gpt1absent glc3man9GlcNAcDNJ added, chromatography, deletion of both
alg6|gpt1decreased glc1man9GlcNAc levelchromatography, deletion of both
alg6|gpt1decreased man9GlcNAc levelchromatography, deletion of both
alg6|gpt1absent glc2man9GlcNAcchromatography, deletion of both
alg6|gpt1absent glc3man9GlcNAcchromatography, deletion of both
gls2|alg6absent glc2man9GlcNAcchromatography, deletion of both
gls2|alg6absent glc3man9GlcNAcchromatography, deletion of both
gls2|alg6increased glc1man9GlcNAcchromatography, deletion of both
gls2|alg6increased man9GlcNAcchromatography, deletion of both
gls2|alg6increased man8GlcNAcchromatography, deletion of both
gpt1|alg6increased RNA levelnorthern, annotation_extension=assayed_using(PomBase:SPAC22A12.15c)
gpt1|alg6increased RNA levelnorthern, annotation_extension=assayed_using(PomBase:SPAC22A12.15c)
gpt1 | alg6lower cell density in stationary phasedeletion, glucose minimal medium, liquid, normal temp
gpt1 | alg6slower cell growth ratedeletion, glucose minimal medium, liquid, normal temp
gpt1 | alg6flocculating cells, microscopydeletion, glucose minimal medium, normal temp
gpt1 | alg6round/swollen cells (they are not small), microscopydeletion, glucose minimal medium, normal temp
gpt1 | alg6slow growth at high temperaturedeletion, glucose minimal medium, normal temp
gls2 | alg6slow aerobic cell growth on glucose carbon sourcenormal glucose MM, high temp, agar plates
gls2 | alg6normal aerobic cell growth on glucose carbon sourcenormal glucose MM, normal temp
gpt1 | alg6slow aerobic cell growth on glucose carbon sourcenormal glucose MM, normal temp, agar plates
gpt1 | alg6slow aerobic cell growth on glucose carbon sourcenormal glucose MM, high temp, agar plates
deletion alg6 | overexpressed gpt1normal cell growthplate, normal glucose MM, normal temp
deletion alg6 | overexpressed gpt1normal cell growthplate, normal glucose MM, high temp
gpt1|alg6normal morphologydeletion, sorbitol added, normal glucose MM, liquid
gpt1|alg6normal cell growth on gluocose carbon sourcedeletion, sorbitol added, normal glucose MM
deletion alg6 | overexpressed gpt1normal morphologynormal glucose MM, high temp
deletion alg6| deletion gpt1|overexpression gpt1(Y1312A,D1352A)round/swollen cellsnormal temp, glucose MM
deletion alg6| deletion gpt1|overexpression gpt1(Y1312A,D1352A)slow growth at high temphigh temp, glucose MM
gls2 | alg6normal morphologynormal glucose MM, normal temp


11016847http://curation.pombase.org/pombe/curs/373f4c6a1e37adf9
yam8 | pck2normal growth at high tempallele=ehs1-1, allele=pck2(overexpressed),high temp, YES
yam8 | pck2slow growth at high tempallele=yam8delta, allele=pck2(overexpressed),high temp, YES
yam8 | pck2normal conjugation freqallele=ehs1-1, allele=pck2(overexpressed)
yam8 | pck2decreased conjugation freqallele=yam8delta, allele=pck2(overexpressed)
yam8 | pck2normal growth on cilofunginallele=ehs1-1, allele=pck2(overexpressed),normal temp, YES
yam8 | pck2sensitive to cilofunginallele=yam8delta, allele=pck2(overexpressed),normal temp, YES
yam8 | pck2normal growth on calcofluor whiteallele=ehs1-1, allele=pck2(overexpressed),normal temp, YES
yam8 | pck2sensitive to calcofluor whiteallele=yam8delta, allele=pck2(overexpressed),normal temp, YES
yam8 | pck2slow growthallele=pck2(overexpression),allele=ehs1-1, solid agar plates, glucose minimal medium, standard temperature
yam8 | pck2normal cell growthallele=pck2(overexpression),allele=ehs1-1, calcium excluded, solid agar plates, glucose minimal medium, standard temperature
yam8 | pck2 normal growthallele=pck2(overexpression),allele=yam8delta, solid agar plates, glucose minimal medium, standard temperature
yam8 | pck2 normal growthallele=pck2(overexpression),allele=yam8delta, calcium excluded, solid agar plates, glucose minimal medium, standard temperature
yam8 | pck2normal level of calcium in cellallele=ehs1-1, allele=pck2(overexpression), normal temp, glucose MM
yam8 | pck2inviableallele=ehs1-1, allele=pck2delta
yam8 | pck2viableallele=yam8delta, allele=pck2delta
yam8 | pck2normal morphologyallele=pck2(overexpression),allele=yam8delta, glucose MM, standard temp microscopy
yam8 | pck2lysed cellsallele=pck2(overexpression),allele=ehs1-1, glucose MM, standard temp microscopy
yam8 | pck1sensitive to calcofluor whiteallele=ehs1-1, allele=pck1(overexpressed),normal temp, YES
yam8 | pck1sensitive to cilofunginallele=ehs1-1, allele=pck1(overexpressed),normal temp, YES
yam8 | pck1slow growth at high tempallele=ehs1-1, allele=pck1(overexpressed),high temp, YES


12896976
pap1 | hba1 sensitive to caffeine pap1 deleted, hba1 overexpressed, standard glucose MM, standard temp, agar plates
pap1 | hba1 sensitive to caffeine pap1 deleted, hba1 deleted, standard glucose rich medium, standard temp, agar plates
sty1 | hba1 resistant to caffeine sty1 deleted, hba1 deleted, standard glucose MM (yeah,diff from above), standard temp, agar plates


756560807cc7abfbaa6d1b0
rds1delta | ade6-D1normal phenotype


18071249http://curation.pombase.org/pombe/curs/5bff0cacfd0d068a
lcf1 | lcf2decreased myristoyl-ligase activitydouble deletion
lcf1 | lcf2abolished palmitoyl-ligase activitydouble deletion
lcf1 | lcf2abolished oleoyl-ligase activitydouble deletion
lcf1 | lcf2decreased viability in stationary phasedouble deletion, standard glucose MM
lcf1 | lcf2decreased viability in stationary phasedouble deletion, growth to stationary phase in rich medium followed by transfer to H2O
lcf1 | lcf2decreased viability in stationary phasedouble deletion, growth to stationary phase in rich medium followed by transfer to H2O
lcf1 | lcf2decreased cell density in stationary phaseallele= double deletion, growth to stationary phase in rich medium followed by transfer to minimal medium


21811607http://curation.pombase.org/pombe/curs/db6b03f9221f5e4a/
pkd2 | pmr1normal immediate intracellular calcium spike following extracellular calcium stimulusenzyme assay,allele=overexpression pkd2 / deletion(pmr1)
pkd2 | pmc1normal immediate intracellular calcium spike following extracellular calcium stimulusenzyme assay,allele=overexpression pkd2 / deletion(pmc1)
pkd2 | vcx1normal immediate intracellular calcium spike following extracellular calcium stimulusenzyme assay,allele=overexpression pkd2 / deletion(vcx1)
pek1 | cch1normal calcium level in response to salt stresspek1DD(unknown), overexpression, cch1delta, enzyme assay
pek1 | yam8normal calcium level in response to salt stresspek1DD(unknown), overexpression, yam8delta, enzyme assay
pek1 | yam8normal calcium level in response to salt stresspek1DD(unknown), overexpression, yam8delta, FK506 added, enzyme assay
1322 | ppb1normal cellular response to salt stressoverexpression of 1322 and deletion of ppb1, cell growth assay, Na ions OR magnesium ions added
1322|prz1decreased immediate intracellular calcium spike following extracellular calcium stimulus 1322 overexpressed, prz1delta
1322|ppb1absent immediate intracellular calcium spike following extracellular calcium stimulus 1322 overexpressed, ppb1delta
pkd2|prz1decreased immediate intracellular calcium spike following extracellular calcium stimulus pkd2 overexpressed, prz1delta
pkd2|prz1decreased immediate intracellular calcium spike following extracellular calcium stimulus pkd2 overexpressed, ppb1delta
1322|pek1normal cellular calcium level during salt stress1322-delta, pek1DD(unknown)
1322|pek1normal cellular calcium level during salt stress1322-delta, pek1DD(unknown), tacrolimus added
trp1322 | pmr1normal prolonged cellular calcium level 1322overexpression, pmr1delta, enzyme assay
pkd2 | pmr1normal prolonged cellular calcium level pkd2overexpression, pmr1delta, enzyme assay
yam8 | cch1increased cellular calcium level in response to sodium ionsdoubledeletion, enzyme assay


9573052
pom1|cdc25monopolar actin cortical patch localization allele=pom1delta, allele=cdc25-22, rhodamine-phalloidin added, high temp
pom1|cdc10monopolar actin cortical patch localization to either end allele=pom1delta, allele=cdc10-V50, rhodamine-phalloidin added, high temp
pom1|cdc25activation of monopolar cell growth at either endallele=pom1delta, allele=cdc25-22, rhodamine-phalloidin added, high temp
pom1|cdc10activation of monopolar cell growth at either endallele=pom1delta, allele=cdc10-V50, rhodamine-phalloidin added, high temp
pom1|cdc11branched cellspom1delta,cdc11-136, high temp, calcofluor added
pom1|act1slow growthpom1delta, act1-48, at high, low and standard temp
pom1|cdc3slow growthpom1delta, cdc3-6, low temp, standard temp
pom1|cdc8slow growthpom1delta, cdc8-110, low temp, standard temp
pom1|nda3slow growthpom1delta, nda3-KM311, standard temp
pom1|ban5slow growthpom1delta, ban5-3, high temp, standard temp
pom1|cdc14slow growthpom1delta, cdc14-118
pom1|mid1slow growthpom1delta, dmf1-6
pom1|tea1viablepom1delta, tea1delta, high temp, standard temp, low temp
pom1|tea1branched cellspom1delta, tea1delta, high temp, standard temp, low temp
pom1|tea1mislocalized septumpom1delta, tea1delta


10224084http://curation.pombase.org/pombe/curs/169c0f8873eee853
hmt2|sir1normal cellular acid labile sulfide (S2-) levelhmt2JS563(E396K)|sir1delta, rich medium


10223994http://curation.pombase.org/pombe/curs/b0116284c3e493a0
tps1|ntp1resistant to freeze-thawingallele=tps1(overexpressed),ntp1(deletion), EMM, solid agar, growth to exponential phase
tps1|ntp1resistant to dehydrationallele=tps1(overexpressed),ntp1(deletion), EMM, solid agar, growth to exponential phase
tps1|ntp1resistant to ethanolallele=tps1(overexpressed),ntp1(deletion), EMM, solid agar, growth to exponential phase
tps1|ntp1increased trehalose level during veg growthallele=tps1(overexpressed),ntp1(deletion), MM, growth to exponential phase
tps1|ntp1increased trehalose level in response to heat shockallele=tps1(overexpressed),ntp1(deletion), MM, heat shock 30 mins 48 degrees, growth to exponential phase
tps1|ntp1resistance to heat shockCGA, allele=tps1(overexpressed),ntp1(deletion), MM, heat shock 30 mins 48 degrees, liquid culture, growth to exponential phase


9532803http://curation.pombase.org/pombe/curs/a826f97af4e46f8f
pap1|rpn11sensitive to brefeldin Apap1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|rpn11normal response to staurosporinepap1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|rpn11sensitive to K-252apap1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|rpn11sensitive to actinomycin Dpap1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|rpn11sensitive to cytochalasin Bpap1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|bfr1resistant to brefeldin Apap1delta, bfr1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|bfr1resistant to staurosporinepap1delta, bfr1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|bfr1resistant to K-252apap1delta, bfr1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|bfr1resistant to actinomycin Dpap1delta, bfr1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
pap1|bfr1resistant to cytochalasin Bpap1delta, bfr1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|rpn11sensitive to brefeldin Abfr1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|rpn11resistant to staurosporinebfr1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|rpn11sensitive to K-252abfr1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|rpn11sensitive to actinomycin Dbfr1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|rpn11sensitive to cytochalasin Bbfr1delta, rpn11-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|pap1sensitive to brefeldin Abfr1delta, pap1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|pap1resistant to staurosporinebfr1delta, pap1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|pap1sensitive to K-252abfr1delta, pap1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|pap1sensitive to actinomycin Dbfr1delta, pap1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added
bfr1|pap1normal cellular response to cytochalasin Bbfr1delta, pap1-overexpressed, CGA, standard temp, liquid medium, EMM, SDS added


16262791|http://curation.pombase.org/pombe/curs/722def8c5161e3f1/
tsc2|rhb1normal cellular response to canavanineallele=tsc2delta,rhb1(D60K), CGA, agar plates, emm


18637840http://curation.pombase.org/pombe/curs/b719dc2cd003f460
zrt1 | zhf1reduced cellular zinc levelliquid scintillation counter/AAS/substance quantification, both deleted, EMM, zinc added
zrt1 | zhf1 sensitive to zinc and zinc starvation both deleted, liquid, standard temp
zrt1 | zhf1 sensitive to zinc and zinc starvation both deleted, agar, standard temp, emm
zrt1 | zhf1normal cellular copper levelspectroscopy, EMM, zinc added, both deleted
zrt1 | zhf1normal cellular potassium levelspectroscopy, EMM, zinc added, both deleted
zrt1 | zhf1normal cellular iron levelspectroscopy, EMM, zinc added, both deleted
zrt1 | zhf1normal cellular manganese levelspectroscopy, EMM, zinc added, both deleted
zrt1 | zhf1normal cellular magnesium levelspectroscopy, EMM, zinc added, both deleted
zrt1 | zhf1normal cellular molybdenum levelspectroscopy, EMM, zinc added, both deleted
zrt1 | zhf1normal cellular nickel levelspectroscopy, EMM, zinc added, both deleted
zrt1 | zhf1normal cellular phosphorus levelspectroscopy, EMM, zinc added, both deleted
zrt1 | zhf1normal cellular sulfur levelspectroscopy, EMM, zinc added, both deleted
zrt1 |spcc126.09 sensitive to zinc starvationallele=deletion, emm, limited zinc added


18662319http://curation.pombase.org/pombe/curs/6dc480135e507182/
cys11 | pgt1 normal cell growth both deleted, emm, plates, cysteine added
cys11 | isp4 normal cell growth both deleted, emm, plates, cysteine added
cys11 | spcc1840.12 normal cell growth both deleted, emm, plates, cysteine added|
cys11 | pgt1 inviable veg cells both deleted, emm, plates, GSH added
cys11 | isp4 normal cell growth on glucose carbon source both deleted, emm, plates, GSH added
cys11 | spcc1840.12 normal cell growth on glucose carbon source both deleted, emm, plates, GSH added
cys11 | pgt1 decreased cellular glutathione level both deleted, limited sulphur added, GSH added


9852154http://curation.pombase.org/pombe/curs/67631dafe77debd1
cdc25-22|pom1-delta1normal protein localizationmicroscopy, emm, high temp, annotation_extensino=assayed_using(PomBase:SPAP8A3.08)
cdc25-22|mid1-deltaFabnormal protein localizationmicroscopy, emm, high temp, annotation_extensino=assayed_using(PomBase:SPAP8A3.08)
cdc25-22|mid1-deltaFabnormal contractile ring localizationmicroscopy, emm, high temp, annotation_extensino=assayed_using(PomBase:SPAP8A3.08)
plo1-1|mid1-deltaFdecreased cell growthCGA, YES, low temp, agar
plo1-1|mid1-deltaFdecreased cell growthCGA, YES, standard temp, agar
plo1-1|mid1-deltaFdecreased cell growthCGA, YES, high temp, agar
plo1-24c|mid1-deltaFdecreased cell growthCGA, YES, low temp, agar
plo1-24c|mid1-deltaFdecreased cell growthCGA, YES, standard temp, agar
plo1-24c|mid1-deltaFdecreased cell growthCGA, YES, high temp, agar
pom1-1|mid1-deltaFinviable germinating spore, no cell divisionsCGA, YES, low temp, agar
pom1-1|mid1-deltaFinviable germinating spore, no cell divisionsCGA, YES, standard temp, agar
pom1-1|mid1-deltaFinviable germinating spore, no cell divisionsCGA, YES, high temp, agar
pom1-1|plo1-1decreased cell growthCGA, YES, low temp, agar
pom1-1|plo1-1decreased cell growthCGA, YES, standard temp, agar
pom1-1|plo1-1inviableCGA, YES, high temp, agar
pom1-1|plo1-24cnormal cell growthCGA, YES, low temp, agar
pom1-1|plo1-24cdecreased cell growthCGA, YES, standard temp, agar
pom1-1|plo1-24cinviableCGA, YES, high temp, agar


22840777http://curation.pombase.org/pombe/curs/f94801dfb551d839
spbc530.05| pap1decreased RNA level during cellular response to purvalanol Adeletion, quant pcr, assayed using bfr1