How BioGRID's notation maps to what we want to display in PomBase web pages

Interactor A = subject (in chado) = "bait"

Interactor B = object (chado) = "hit" or "prey"

Also see BiogridSymmetry

BioGRID evidence	Symmetric?	Interactor A (bait, subject)	Interactor B (hit, prey, object)
Co-crystal Structure	Y	co-crystallizes with	co-crystallizes with
Co-fractionation	Y	co-fractionates with	co-fractionates with
Co-localization (not used by PomBase)	Y	n/a (would be co-localizes with)	n/a (would be co-localizes with)
Co-purification	Y	co-purifies with	co-purifies with
Reconstituted Complex	Y	forms complex with	forms complex with
Affinity Capture-Luminescence	N	affinity captures	affinity captured by
Affinity Capture-MS	N	affinity captures	affinity captured by
Affinity Capture-RNA	N	affinity captures	affinity captured by
Affinity Capture-Western	N	affinity captures	affinity captured by
Biochemical Activity (not used by PomBase)	N	n/a (would be modifies)	(would be modified by)
Far Western	N	captures	captured by
FRET	N	fluorescence resonance energy donor to	fluorescence resonance energy acceptor from
PCA	N*	interacts with	interacts with
Protein-peptide	N	binds to peptide	(peptide) binds to protein
Protein-RNA	N	binds to RNA	binds to protein
Two-hybrid	N	binds activation domain construct with	binds DNA-binding domain construct with
Negative Genetic	Y	negative genetic interaction with	negative genetic interaction with
Positive Genetic	Y	positive genetic interaction with	positive genetic interaction with
Synthetic Growth Defect	Y	synthetic growth defect with	synthetic growth defect with
Synthetic Haploinsufficiency	Y	synthetic haploinsufficient with	synthetic haploinsufficient with
Synthetic Lethality	Y	synthetic lethal with	synthetic lethal with
Synthetic Rescue	N	synthetically rescued by	synthetically rescues
Dosage Growth Defect	N	growth defect in presence of overexpressed	overexpression causes growth defect to
Dosage Lethality	N	inviable in presence of overexpressed	overexpression lethal to
Dosage Rescue	N	rescued by overexpression of	overexpression rescues
Phenotypic Enhancement	N	phenotype enhanced by	enhances phenotype of
Phenotypic Suppression	N	rescued by	rescues

• PCA is not exactly symmetric, since there will be one N-terminal and one C-terminal reporter fusion construct, but we're not going to capture that. PCA can therefore be handled the same way as the symmetric interactions in the curation tool.