Changes between Version 31 and Version 32 of CapturingGeneExpression


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Timestamp:
Oct 17, 2012, 1:35:23 PM (9 years ago)
Author:
antonialock
Comment:

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  • CapturingGeneExpression

    v31 v32  
    3434|| mRNA level|transcription|degradation|protein level|translation|degradation || during phase or process (S, phase, meiosis, sporulation, cellular response to X)|in_response_to(CHEBI chemical) ||increased|decreased|| ECO:... ||
    3535
    36  * If a gene is present in a blot under normal growth conditions I use extension during(vegetative growth of single celled organism) + qualifier=present. I then set increased/decreased during other responses
     36 * If a gene is present in a blot under normal growth conditions I use extension during(vegetative growth of single celled organism) + qualifier=present. I then set increased/decreased during other responses (veg growth may not be the optimal term to use but I've used it in the context of when growing in standard medium, rather than having elevated levels of some nonstandard compound in there, or starved of something, or any other arbitrarily abnormal condition)
     37 * if they don't show the level during vegetative growth use 'present' or 'absent' during a condition (nothing to compare to)
     38 * use 'unchanged' if level doesn't change during a condition
    3739 * I use 'in_response_to(CHEBI chemical)' if there isn't a GO 'response to' term for the chemical
    3840
     
    4749||ECO:0000106||Northern assay evidence||
    4850||ECO:0000049||reporter gene assay||
     51||ECO:0000108||RT-PCR||
    4952
    5053