Changes between Version 64 and Version 65 of CapturingGeneExpression


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Timestamp:
Jan 27, 2015, 2:49:40 PM (6 years ago)
Author:
vw253
Comment:

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  • CapturingGeneExpression

    v64 v65  
    1313||RNA level ||A northern blot (for instance) can only tell you if there is RNA there||
    1414||protein level|-|||
    15 || transcription||Requires a specific assay for transcription, not only presence of RNA, as could be decreased degradation|\
     15|| transcription||Requires a specific assay for transcription, not only presence of RNA, as could be decreased degradation||
    1616||RNA degradation|| Requiers a specific assay for degradation||
    1717||translation|| -||
    1818||protein degradation||-||
    1919
    20   * '''Expression type''' Allowed types: RNA level, protein level, transcription, RNA degradation, translation, protein degradation
    21    * A northern blot (for instance) can only tell you if there is RNA there, and if the amount changes during different conditions. Other assays may tell you specifically if it is transcription or degradation that is affected (for instance, the amount of RNA could increase due to increased transcription or decreased degradation).
    22   * '''Relation''' during(GO biological process ID) or in_presence_of(ChEBI ID), occurs_in(cell type)
     20||Allowed relations||Example || comment||
     21|| during||during(GO biological process ID) ||-||
     22||in_presence_of||In_presence_of(ChEBI ID)||-||
     23||occurs_in(cell type)||-||-||
    2324   * Unstressed conditions include during "vegetative growth of single-celled organism" (=whole cell extract) or "mitotic/meiotic cell cycle" (= they looked at different phases))
    2425   * Unstressed conditions include growth on YE and MM unless there is a known difference between the two (in which case EMM might eg be a starvation condition
     
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