Changes between Version 366 and Version 367 of ChromosomeSegregation


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Timestamp:
Apr 19, 2016, 9:27:46 AM (5 years ago)
Author:
vw253
Comment:

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  • ChromosomeSegregation

    v366 v367  
    209209||top3 DNA topoisomerase III ||-||
    210210||sfi1|| -||-||
     211||mfh1|| one paper ||-||
     212||mfh2|| one paper ||-||
    211213===
    212214||dis1 ||PMID:16920624 plus…XMAP215/ TOG/Stu2-family homolog, binds microtubules and localizes to kinetochores (Nakaseko et al., 2001). Mutants in dis1 lack the chromosome alignment phase in metaphase and arrest with elongated spindles and segregated but unseparated chromosome pairs (Nabe- shima et al., 1998; Ohkura et al., 1988).dis1 mutants arrest with an activated spindle checkpoint and appear to ex- perience an increase in sister chromatid cosegregation (70%) at full restrictive temperature. Such cosegregation results when mono-oriented attachment of sister kineto- chores to microtubules from the same SPB is not corrected. Since dis1 mutants lack the phase of constant spindle length when chromosomes are properly aligned ||-||
     
    214216||cut7||In the temperature-sensitive cut7 mutant, spindle formation is blocked shortly after duplication of the SPB. The mitotic spindle radiating from the opposite poles cannot interdigitate and thus remains as a V shape (Hagan and Yanagida, 1992). The cut7- specific defect generates a condition whereby the bipolar attachment of the spindle to kinetochores is not achieved. Previously, we demonstrated that this defect causes an arrest in a mad2+-dependent manner (Kim et al., 1998). At the restrictive temperature the majority of Mad2-GFP was found on condensed chromosomes as one or more speckles (Fig. 4).||
    215217||skp1||PMID:15147872||-||
    216 ||mfh1|| one paper ||-||
     218
    217219
    218220 === Notes and annotation questions on chromosome segregation ===