wiki:ChromosomeSegregation

Version 147 (modified by vw253, 6 years ago) (diff)

--

Annotation Priorities

  • Processes relevant to the most , (especially anything related to cell cycle regulation, cancer, broadly conserved)
  • Have large percentage of functionally characterised genes (for example mitochondrion organization has 40 published genes/294, so is low priority compared to cytokinesis 136/139)
  • Are mature enough to be able to construct pathways/networks
  • New gene characterizations -These should be sent out for community curation, but followed up
  • phenotype screens see canto
  • Process priorities : chromosome segregation, DNA metabolism, cytokinesis, regulation of mitotic cell cycle, any signaling pathway, chromatin organization, , cytokinesis, establishment or maintenance of cell polarity, protein modification by small protein conjugation or removal
  • kinases, phosphatases, GTPAses, ubiquitin ligases, protein methylases (anything with direct substrate to improve networks)

HIGHEST priority genes/complexes/papers

(may be in one of the other dsc papers though....) chung paper?
mus81 PMID:15805465 Mus81-T239A disrupts the ability of Cds1 to bind and phosphorylate Mus81 and causes hyper-recombination upon hydroxyurea-induced replication arrest
cdc2PMID:8557037mcs6 substrate cdc2
cdc2PMID:6526270uniprot has this paper for allele cdc2-4w C67F (we have 'unspecified')
cdc2 Millar JB et al. (1991) -
cdc2 Moreno S et al. (1989) -
cdc2 Simanis V et al. (1986) -
cdc2 Booher RN et al. (1989) -
cdc2 Noguchi E et al. (2002 -
Clp1PMID:18378776 Clp1 in turn dephosphorylates the essential F-BAR scaffold protein Cdc15, CR assembly
plo1?????mid is a substrate
Sid2Chen et al. 2008phosphorylates the nonessential Cdc14 phosphatase Clp1 to foster Clp1’s cytoplasmic retention
Mre1 complexPMID:21008122 and papers cited within In humans and both yeasts, DSBs are initially detected and processed by the 1Xrs2 (MRN) nucleolytic protein complex in association with the Tel1ATM checkpoint kinase and the Ctp1CtIP/Sae2 DNA-end processing factor;...implies tel1 /rad50/nbs1 should be annotated to GO:0000729 The functions of ATM and ATR orthologs are intimately tied to the detection and nucleolytic processing of DSBs. ATMTel1 localizes at DSBs by interacting with (MRN) protein complex, which directly binds DNA ends (12, 20, 24, 50, 52). use these papers to annotate the missing connection between tel1 and the mre11 complex
crb2PMID:14739927 Finally, we show direct interaction between Rad3 and Crb2, which is inhibitory to Rad3 activity (direct enzyme regulator substrate)
crb2PMID:21098122 There are two pathways that lead to Crb2 localization at sites of DNA damage (10). One pathway requires two inde- pendent histone modifications: (i) phosphorylation of the C- terminal tail of histone H2A
Tel1 PMID:21098122 Tel1 phosphorylation of histone H2A at DSBs depends on its interaction with the Nbs1 subunit of MRN complex (52). (add tel1 targets as histones)
crb2 ????Find physical interaction with Rad9
All histones????These are often not annotated with their roles in cell cycle regulation and repair, and so they are excluded from the graphs (also need to have specific nucleosome terms, 'what do you call a normal nucleosome?l' and 'centromere specific'
srw PMID:9571240, PMID:9736616 PMID:10921878 has no mitotic cell cycle regulation annotation
fnx1 & 2PMID: 18503766 This wouldn't normally be a priority, but I'm putting it in becasue the exisitng transport GO annotations look a bit of a mess (stretch) ... need to get detailed phenotypes and then decide what it is possible to infer sensibly
APC (both complexes, cdc25 & fizzy like ??????-
Scf complex ?????-
scp1???Skp1 doesn't have ubiquitin ligase activity so these MF annotations should probably be on BP ???-
dsc2??? activation of sre2 TF? positive regulation of SREBP signaling pathway by transcription factor cleavage? for the Dsc subunits?

phosphatase1703321,

Process priorities

Chromosome segregation (Val, but feel free to do any genes or complexes that I haven't started)

process chromosome segregation: genes 207 of which 183 published esyN
gene/complexstatus date
fta1 Mis6-Sim4 complex annotation complete6/1/2015
fta2 Mis6-Sim4 complex annotation complete 6/1/2015
fta3 Mis6-Sim4 complex annotation complete 6/1/2015
fta4 Mis6-Sim4 complex annotation complete 15/1/2015
fta6 Mis6-Sim4 complex annotation complete 15/1/2015
fta7 Mis6-Sim4 complex annotation complete except CC PMID: 2478970820/1/2015
mis6 Mis6-Sim4 complex annotation complete except CC PMID:24789708 PMID2537524020/1/2015
mis15 Mis6-Sim4 complex annotation complete 6/1/2015
mal2 Mis6-Sim4 complex PMID: 12242294 + others -
sim4 Mis6-Sim4 complex annotation complete 20/1/2015
sos7 NMS complex annotation complete 6/1/2014
spc25 NMS complex/ Ndc80 complexannotation complete 20/1/2015
spc7 NMS complex in progress IS THIS TURNING SAC SIGNALLING OFF? PMID: 17035632-
ndc80 NMS complex/Ndc80 annotation complete except CC PMID:21256022 -
mis12 NMS complexto do PMID:15930132 check biorientation ann-
mis13 NMS complexannotation complete 20/1/2015
mis14 NMS complexannotation complete 6/1/2014
nnf1 NMS complex to do PMID:17035632 -
mis16 CENP-A recruiting complex complete except PMID:22771823 (out for CC) and scm3 papers-
mis18 CENP-A recruiting complex complete except PMID:24789708 (out for CC) -
mis19 CENP-A recruiting complex complete except PMID:25375240 (out for CC) -
mis20 CENP-A recruiting complex annotation complete 20/1/2015
nuf2 Ndc80 complex 2 plus PMID:23770679 (out for CC)-
spc24 Ndc80 complex annotation complete20/1/2015
dad1 DASH complex to do PMID:16079914 PMID:16079915 -
csm1 monopolin complex in progress PMID: 20935472 plus…-
mde4 monopolin complex in progress-
hhp1 monopolin complex in progress-
hhp2 monopolin complex in progress-
mis17 annotation complete 6/1/2015
cnp20 annotation complete 6/1/2015
dis1 PMID:16920624 plus… -
ppe1 to do PMID: 12773390 -|
scm3 to do PMID: 19217403 PMID: 19217404 (also mis16)-
cnp1 in progress-
mal3 in progress-
dad1 PMID: 17352737 -
cnp3PMID: 15791413 PMID: 24449889 autonomous-
sds22 phoshatase PMID: 1846086 PMID: 15335873 PMID: 8374168 -
cnd1 condensin complex non-SMC subunit PMID: 18362178-
cnd2 condensin complex non-SMC subunit --
cnd3 condensin complex non-SMC subunit --
hos2 17352737 plus -
pcs1 PMID:20935472 plus... -
ams12 chen et al, cenp-a loading -
nsk1 abnormal clustering of kinetochores to spindle pole bodies-

other chromosome segregation

dis2 phosphatase (PMID:14765108 - describes checkpoint termination Dis2 abrogates Chk1 phosphorylation and activation in vivo, ) PMID:2245912 PMID:8389306 PMID:8305731 PMID:7983142 PMID:7957097 PMID:9264466 PMID:9790575 PMID:10668632 PMID:11085271 PMID:16411888 PMID:17895368 PMID:19592249 PMID:21664573 (Out for CC PMID:21703453 PMID:21965289 PMID:23333317) (Iain Hagan PMID:25487150) -
dis1 XMAP215/ TOG/Stu2-family homolog, binds microtubules and localizes to kinetochores (Nakaseko et al., 2001). Mutants in dis1 lack the chromosome alignment phase in metaphase and arrest with elongated spindles and segregated but unseparated chromosome pairs (Nabe- shima et al., 1998; Ohkura et al., 1988).dis1 mutants arrest with an activated spindle checkpoint and appear to ex- perience an increase in sister chromatid cosegregation (70%) at full restrictive temperature. Such cosegregation results when mono-oriented attachment of sister kineto- chores to microtubules from the same SPB is not corrected. Since dis1 mutants lack the phase of constant spindle length when chromosomes are properly aligned

Notes and annotation questions on chromosome segregation

  • read review PMID: 23512483
  • is CENP-A containing nucleosome assembly part of kinetochore assembly? Although the outer repeats play an important role in sister centromere cohesion and possibly help to properly orient the multiple kinetochore microtubule attachment sites (Pidoux and Allshire, 2004
  • Centromeres: getting a grip of chromosomes. Curr. Opin. Cell Biol. 12, 308–319., the central region is needed for the assembly of the kinetochore per se and the interaction with the mitotic spindle fibers
  • what is the relationship between kinetochoe assembly, spindle elongation and chromosome segregation (currently disjoint)
  • reannotate ndc80 and MIND subcomplexes annotate PAS complex

Cytokinesis but feel free to do any genes or complexes that I haven't started

process cytokinesis: 139 of which 136 published esyN
gene/complexstatus date
lsk1 in progress-
mac1 annotation complete 22/1/2014
lkh1 chained 12565823 -

Notes and annotation questions on cytokinesis

  • previously considered to be linear assembly mid1- rng2- others. But if you can ectopically localize rng2 you can get all of the others (any one of the 3 will work)

check should mid1 be ring assemlby or localization only?

check mid1 NOT assembly? positioning
main target of mid1 is rng2 rng3- myosin specific chaperone add to wiki with mohan paper details

Regulation of mitotic cell cycle

process regulation of mitotic cell cycle: l genes 258 of which 231 published esyN
gene/complexstatus date

Notes and annotation questions on cell cycle regulation

  • Only include gene products here not covered in chromosome segregation, cytokinesis or DNA metabolism
  • genes which should be annotated to "signalling" based on the signalling components of the cell cycle regulation network
  • Need to do cytokinesis checkpoint, G1 cell size control checkpoint, DNA damage checkpoint (ask experts to check specific checkpoint lists)