Annotation Priorities

Overall annotation priorities

Think about processes which are:

• Relevant to the majority of pombe labs
• More interest to researchers from other organisms, (especially humans, and cancer), anything related to cell cycle regulation
• Have large percentage of functionally characterised genes (for example mitochondrion organization has 40 published genes/294, so is low proiority compared to cytokinesis 136/139)
• Are mature enough to be able to construct pathways/networks

phenotype screens

• see ​canto (big bang for buck, ups numbers)

Function pririties priorities Targets of gene products (for improving networks) priority papers

• Mus81-T239A disrupts the ability of Cds1 to bind and phosphorylate Mus81 and causes hyperrecombi- nation upon hydroxyurea-induced replication arrest (Kai et al., 2005) PMID: 15805465 .
• mcs6 substrate cdc2 PMID: 8557037

Process priorities

Process priorities : chromosome segregation, DNA metabolism, cytokinesis, regulation of mitotic cell cycle, ascospore formation, autophagy (quick and good GO terms), cytokinesis, establishment or maintenance of cell polarity. protein modification by small protein conjugation or removal

process chromosome segregation: main curator val genes 207 of which 183 published ​esyN
gene/complex	status	date
fta1 Mis6-Sim4 complex	annotation complete	6/1/2014
fta2 Mis6-Sim4 complex	annotation complete	6/1/2014
fta3 Mis6-Sim4 complex	annotation complete	6/1/2014
fta4 Mis6-Sim4 complex	in progress	-
fta6 Mis6-Sim4 complex	in progress	-
fta7 Mis6-Sim4 complex	in progress	-
mis6 Mis6-Sim4 complex	in progress	-
mis15 Mis6-Sim4 complex	annotation complete	6/1/2014
mal2 Mis6-Sim4 complex	PMID: 12242294 + others	-
sim4 Mis6-Sim4 complex	in progress	-
sos7 NMS complex	annotation complete	6/1/2014
spc25 NMS complex/ Ndc80 complex	to do PMID:15728720	-
spc7 NMS complex	in progress IS THIS TURNING SAC SIGNALLING OFF?	-
ndc80 NMS complex/Ndc80	to do PMID:21256022 PMID:15728720	-
mis12 NMS complex	to do PMID:7865880 PMID:10398680 PMID:10761928 PMID:10864871 PMID: 15502821 PMID: 15930132 PMID: 16079915	-
mis13 NMS complex	to do PMID:18362178	-
mis14 NMS complex	annotation complete	6/1/2014
nnf1 NMS complex	to do PMID:17035632	-
mis16 CENP-A recruiting complex	complete except PMID:22771823 (out for CC) and scm3 papers	-
mis18 CENP-A recruiting complex	complete except PMID:24789708 (out for CC)	-
mis19 CENP-A recruiting complex	complete except PMID:25375240 (out for CC)	-
mis20 CENP-A recruiting complex	in progress	-
nuf2 Ndc80 complex	in progress	-
spc24 Ndc80 complex	annotation complete (when the ndc80 done)	-
dad1 DASH complex	to do PMID:16079914 PMID:16079915	-
csm1 monopolin complex	in progress PMID: 20935472 plus…	-
mde4 monopolin complex	in progress	-
hhp1 monopolin complex	in progress	-
hhp2 monopolin complex	in progress	-
mis17	annotation complete	6/1/2014
cnp20	annotation complete	6/1/2014
dis1	PMID:16920624 plus…	-
ppe1	to do PMID: 12773390	-|
scm3	to do PMID: 19217403 PMID: 19217404 (also mis16)	-
cnp1	in progress	-
mal3	in progress	-
cnp3	PMID: 15791413 PMID: 24449889 autonomous	-

Notes and annotation questions on chromosome segregation

• read review PMID: 23512483
• is CENP-A containing nucleosome assembly part of kinetochore assembly? Although the outer repeats play an important role in sister centromere cohesion and possibly help to properly orient the multiple kinetochore microtubule attachment sites (Pidoux and Allshire, 2004
• Centromeres: getting a grip of chromosomes. Curr. Opin. Cell Biol. 12, 308–319., the central region is needed for the assembly of the kinetochore per se and the interaction with the mitotic spindle fibers

=Cytokinesis=

process cytokinesis: main curator val genes 139 of which 136 published ​esyN

gene/complex	status	date
lsk1	in progress	-

Notes and annotation questions on cytokinesis

Regulation of mitotic cell cycle

process regulation of mitotic cell cycle: l genes 258 of which 231 published ​esyN
gene/complex	status	date