wiki:ComplementationAnnotations

Version 122 (modified by antonialock, 7 years ago) (diff)

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syntax used for complementation

full complementationqualifier=full
partial complementationqualifier=partial
pombe gene X complements loss of homologous gene in other organism Yfunctionally complements organismY geneX
loss of pombe gene is complemented by gene X from another organism Yfunctionally complemented by organismY geneX
no complementation observeddoes not functionally complement organismY + geneX
no complementation observedis not functionally complemented by organismY + geneX
example string/controlled_curation="term=complementation, functionally complements S. cerevisiae GSH2; qualifier=partial; db_xref=; date="

pombe gene Complements

PMID Pombe gene/allele Organism complemented gene complemented phenotypes suppressed
PMID:1327149tmp1 S. cerevisiae cdc8-1 complements cell cycle defect Val
PMID:1922021tbp1 S. cerevisiae SPT15 complements growth defect Val
PMID:2197558tbp1 S. cerevisiae SPT15 complements growth defect Val
PMID:2227411suc1 S. cerevisiae cks1-null complements ? Val
PMID:3063400 leu1 E. coli leuB Val
PMID:3063400 leu1 S. cerevisiae leu2 Val
PMID:9559549 spt3 S. cerevisiae spt3-delta partially complements Val
PMID:9693378 mam4 S. cerevisiae Deltaste14 complements Val
PMID:10050047 hba1 S. cerevisiae Deltayrb2 rescues cold sensitivity Val
PMID:1448066 ade2 E. coli purA E. coli carrying a mutation in purA can grow in MM-adenine when transformed with pombe ade2 AL
PMID:8663290 rip1 S. cerevisiae RIP1 RIP1 deletion mutant can grow on a non-fermentable carbon source (2% glycerol) when expressing pombe rip1
PMID:8663290 rip1 S. cerevisiae RIP1 pombe rip1 restores 25-30% of ubiquinol-cytochrome c reductase activity in cerevisiae
PMID:8663290 rip1 S. cerevisiae RIP1 pombe rip1 restores 65% of succinate-cytochrome c reductase activity in mitochondrial membrane in cerevisiae
PMID:8663290 rip1 S. cerevisiae RIP1 pombe rip1 restores 70% of NADH-cytochrome c reductase activity in mitochondrial membrane in cerevisiae
PMID:1409592 ura3 S. cerevisiae URA1 pombe ura3 makes cerevisiae ura1-delta ura+ when grown in an aerobic condition. Cells cease growth however under anaerobic conditions (WT cerevisiae can grow anaerobically unlike pombe)AL
PMID:10712694 oxa101 S. cerevisiae OXA1 Cerevisiae cells transformed with pombe oxa101 can grow on glycerol which oxa1 mutants in cerevisiae normally cannot, however, growth is slower than WT. AL
PMID:10712694 oxa102 S. cerevisiae OXA1 Cerevisiae cells transformed with pombe oxa102 can grow on glycerol which oxa1 mutants in cerevisiae normally cannot, however, growth is slower than wt but better than when complementing with oxa101. AL
PMID:cyc1S. cerevisiae CYC1 + CYC7 cerevisiae double deletion for cyc1 and cyc7 cannot grow on glycerol (which is a non-fermentable carbon source in cerevisiae). S. cerevisiae transformed with Pombe cyc1 can grow on glycerol
PMID:10234787stt3S. cerevisiaeSTT3pombe stt3 suppress temperature sensitivity of cerevisiae sttr3-1 mutation when cells are grown on rich medium. It is not suppressed when grown on minimal medium. Glycosylation defect is also not supressedAL
PMID:8921872mag1E. colialkA/tagpombe mag1 partially restores resistance of an alkA/tag E. coli double mutant to alkylation by MMS/MNNG/ENNG and PNNGAL
PMID:20106903 vrs1 S. cerevisiae VAS1 pombe vrs1 rescues the cytoplasmic form (growth assay) of VAS1 knockout AL
PMID:15075270 liz1 S. cerevisiae FEN2 pombe liz1 rescues growth of fen2 knockout on low pantothenate medium AL
PMID:15075270 vht1 S. cerevisiae VHT1 pombe vht1 rescues growth of VHT1 knockout on low biotin medium AL
PMID:17559414 mpr1 S. cerevisiae Ypd1 delta-ypd1 is lethal. Pombe mpr1 rescues growth. N-terminal truncationst of mpr1 also rescues growth AL
PMID:8765231 gpm1 S. cerevisiae GPM1 cerevisiae deleted for GPM1 cannot grow in YEPD medium. Pombe gpm1 rescues growth AL
PMID:8765231 pma1 S. cerevisiae PMA1 cerevisiae deleted for PMA1 grows small colonies and lacks membrane fraction ATPase activity AL
PMID:2112089 ypt2 S. cerevisiae SEC4 a ts cerevisiae sec4- mutant that cannot grow at high temp is rescued by pombe ypt2 AL
PMID:6294096 adh1 S. cerevisiae ADR1-1, ADR2-deltapombe adh1 complements a cerevisiae strain deficient in alcohol dehydrogenase activity AL
PMID:8879272 rhp16 S. cerevisiae RAD16-deltacerevisiae rad16delta are sensitive to UV and are deficient in nontranscribed dna repair. pombe rhp16 partially rescues survival upon UV radiation and nontranscribed dna repair AL
PMID:9001235 dbr1 S. cerevisiae dbr1intron accumulation defect of a cerevisiae dbr1 mutant is complemented by pombe dbr1 AL
PMID:3912263 tpi1 S. cerevisiae TPI1growth deficiency of s. cerevisiae N582-4B in glucose MM mitigated by pombe tpi1 AL
PMID:8299169 his7 E. coli EcoCyc:EG10451 pombe his7 rescues histidine auxotrophy in an E. coli hisI mutant AL
PMID:23640107 oga1 S. cerevisiae STM1deletion of STM1 in cerevisiae makes them caffeine sensitive. pombe oga1 rescues this sensitivity AL
PMID:11095668 pus1 S. cerevisiae PUS1deletion of PUS1 and LOS1 in cerevisiae makes them cunable to grow at 37 degrees. Pombe pus1 rescues this AL
PMID:3796591 cdc2 S. cerevisiae CDC28 pombe cdc2 fully complements growth defect of cerevisiae cdc28.4 but only when the introns are removed AL
PMID:7657644 cam1 S. cerevisiae CMD1 pombe cam1 supports growth of cerevisiae cmd1 AL
PMID:8675018 anc1 S. cerevisiae ANC1 and ANC2 pombe anc1 supports growth of cerevisiae ANC1 and ANC2 delta on non-fermentable carbon source AL
PMID:9737967 myh1 E. coli MutY pombe myh1 restores mutation rate in e. coli MutY mutant almost to WT level, probably within error of assay. They do have slightly different affinities for substrates though and kinetics AL
PMID:8021171 tps1 S. cerevisiae TPS1 pombe tps1 enables cerevisiae tps1-delta to grow on glucose (growth rate a bit lower than wt) and to have trehalose-6-p synthase activity (though lower than wt) also functional in stress response AL
PMID:11284010 hut1 S. cerevisiae HUT1 pombe hut1 alleviates cerevisiae ero1ts/hut1-delta DTT sensitivity AL
PMID:11754480 SPBC428.11 S. cerevisiae MET17 pombe SPBC428.11 restores O-acetylhomoserine and O-acetylserine sulphydrolase activity in a cerevisiae met17 mutant AL

Exogenous gene Does not complement pombe gene/allele

PMID Pombe gene/allele Organism gene Val
PMID:1577774 cdc25-22 S. cerevisiae PTP2 Val
PMID:1606618 cdc25delta D. melanogaster string Val
PMID:1606618 cdc25delta D. melanogaster twine Val
PMID:1704128 cdc2-? X. laevis Eg1 Val
PMID:1717994 cdc2-? H. sapiens CDK2 Val
PMID:2120045 cdc2-? D. melanogaster Dm cdc2c Val
PMID:8246900 pol1delta H.sapiens POL1 Val
PMID:7923372 scd2-1 S. cerevisiae BEM1 does not restore conjugation nor cell shape Antonia
PMID:10471809tpr1S.cerevisiaeTRK1 and TRK2cerevisiae expressing pombe tpr1 rescues growth of TRK1 and 2 double deletion on low potassium medium. AL
PMID:8887553cdc1S.cerevisiaeHys2expression of hys2 does not rescue pombe cdc1 mutantsAL
PMID:9203579spg1S.cerevisiaeTEM1TEM1 does not rescue heat sensitivity of spg1-B8AL
PMID:8290359rad8S.cerevisiaeRAD5RAD5 does not rescue UV sensitivity of rad8-delta, there is a very minute improvement however, but I didn't think it was convincing enough to call it partialAL
PMID:8290359rad8S.cerevisiaeRAD54RAD54 does not rescue UV sensitivity of rad8-deltaAL
PMID:7657644 cam1 S. cerevisiae CMD1 pombe cam1-delta expressing cerevisiae CMD1 cannot grow AL

pombe gene does not complement gene in other organism

PMID Pombe gene Organism gene Val
PMID:2197558 cdc10 S. cerevisie CDC6 Val
PMID:20106903 vrs2 S. cerevisiae VAS1 pombe vrs1 does not rescue the mitochondrial form of VAS1 (growth on glycerol requires mitochondrial trna ligase) (the cytoplasmic form of vas1 was expressed in this experiment) AL
PMID:15075270 liz1 S. cerevisiae vht1 pombe liz1 does not rescue growth of vht1 knockout AL
PMID:2112089 ypt2 S. cerevisiae YPT1 pombe ypt2 does not complement a cerevisiae YPT1 null mutant AL
PMID:1829983 cig1 S. cerevisiae CLN1/2/3 pombe cig1 cause growth arrest in cerevisiae CLN1/2/3 triple deletion mutant, sporulated cells arrest unbudded AL
PMID:8299169 his7 E. coli EcoCyc:EG10444 pombe his7 does not rescue histidine auxotrophy in an E. coli hisA mutant AL
PMID:8299169 his7 E. coli EcoCyc:EG10445 pombe his7 does not rescue histidine auxotrophy in an E. coli hisB mutant AL
PMID:8299169 his7 E. coli EcoCyc:EG10446 pombe his7 does not rescue histidine auxotrophy in an E. coli hisC mutant AL
PMID:8299169 his7 E. coli EcoCyc:EG10447 pombe his7 does not rescue histidine auxotrophy in an E. coli hisD mutant AL
PMID:8299169 his7 E. coli EcoCyc:EG10448 pombe his7 does not rescue histidine auxotrophy in an E. coli hisF mutant AL
PMID:8299169 his7 E. coli EcoCyc:EG10449 pombe his7 does not rescue histidine auxotrophy in an E. coli hisG mutant AL
PMID:8299169 his7 E. coli EcoCyc:EG10450 pombe his7 does not rescue histidine auxotrophy in an E. coli hisH mutant AL
PMID:3796591 cdc2 S. cerevisiae CDC28 pombe cdc2 does not rescue growth defect of cdc28.4 AL
PMID:21113731 cfr1 S. cerevisiae CHS5 pombe cfr1 does not promote chitin synthesis or cell surface expression of CHS3 in cerevisiae AL

pombe gene/allele Complemented By

PMID Pombe gene/allele Organism gene phenotypes suppressed
PMID:1313771 cdc25-22 M. Musculus Cdc25M2 OEX restoring the temperature-conditional growth defect Val
PMID:1314161 dis2-11 A. thaliana PP1-At restores cs chromosome disjuction phenotype Val
PMID:1606618 cdc25-? D. melanogaster string rescues ts mutant Val
PMID:1606618 cdc25-? D. melanogaster twine rescues ts mutant Val
PMID:2120044 cdc25-22 D. melanogaster string rescues ts mutant Val
PMID:2120044 cdc25-22 D. melanogaster Dm cdc2 rescues ts mutant Val
PMID:2120045 cdc2-? D. melanogaster Dm cdc2 rescues ts mutant Val
PMID:7962194 cdc2-33 A. nidulansnimXcdc2 Val
PMID:7476862 cdc2delta D. melanogaster Dm cdc2 capable of mating and producing four viable meiotic products, responds to nutritional starvation and to incomplete DNA synthesis, produces a 'wee' phenotype, and it is largely insensitive to the action of the S. pombe wee1+ mitotic inhibitor, hypersensitive to UV irradiation Val
PMID:1662986 cdc25-? H. sapiens CDC25Hu2 full rescue of ?? Val
PMID:2195549 cdc25-? H. sapiens CDC25 rescue of cell cycle defect Val
PMID:1937013 cdc2-33 A. thaliana CDC2a rescues cell cycle phenotype Val
PMID:1937013 cdc2-33 A. thaliana CDC2b rescues cell cycle phenotype Val
PMID:2000373 cdc2-?? Medicago sativa L. cdc2Ms rescues ts cell cycle phenotype Val
PMID:1983087 ras1delta H. sapiens RAP1A suppress defects in cell morphology and sporulation, not conjugation Val
PMID:1983087 ras1delta H. sapiens RAP1B suppress defects in cell morphology and sporulation, not conjugation Val
PMID:2663184 rad13 S. cerevisiae RAD2 repair defect Val
PMID:6347688 cdc17 S. cerevisiae cdc9 DNA ligase defect Val
PMID:7565716 vps34-delta D. discoideum PIK1 complemented the temperature-sensitive growth defect Val
PMID:7589895 fbp1 R. norvegecus fbp2 complemented fructose-1,6-bisphosphatase (Fru-1,6-Pase) activity Val
PMID:7808417 wee1-50/mik1delta D. melanogaster Hel25E mitotic catastrophe Val
PMID:7808417 wee1-50/OEXcdc25 D. melanogaster Hel25E mitotic catastrophe Val
PMID:8573790 wee1-50/mik1 delta D. melanogaster Dwee1 mitotic catastrophe Val
PMID:7816619 pde1-mutant ?A. thalianaAt PP2Csterile phenotype, defective in cAMP phosphodiesterase Val
PMID:7816619 pde1-mutant ?A. thalianaAt Dr1sterile phenotype, defective in cAMP phosphodiesterase Val
PMID:7816619 pde1-mutant ?A. thalianaTBPsterile phenotype, defective in cAMP phosphodiesterase Val
PMID:7816619 pde1-mutant ?S. cerevisieTBPsterile phenotype, defective in cAMP phosphodiesterase Val
PMID:7885830 dhp1-ts ? M. musculus Dhm1 rescued the temperature-sensitivity Val
PMID:7885830 dhp1delta M. musculus Dhm1 rescued lethality Val
PMID:7962207 cap1 H. sapiens CAP elongation (CC) phenotype, propensity to sporulate Val
PMID:7962207 cap1 H. sapiens CAP2 elongation (CC) phenotype Val
PMID:8007985 rad2 H. sapiens FEN1 UV sensitivity Val
PMID:8041632 cdc2-L7 H. sapiens scr2 Val
PMID:8041632 cdc2-L7 H. sapiens scr3 Val
PMID:8041632 cdc13-117 H. sapiens scr2 Val
PMID:8041632 cdc13-117 H. sapiens scr3 Val
PMID:8045419 wee1 several X. laevis ??? Val
PMID:8089179 gtb1 H. sapiens TUBG1 growth defect/ microtubule defects Val
PMID:8106391 mutant25 ??? C. kessleri HUP1 glucose uptake deficiency Val
PMID:8552193 ??? X. laevis XORC2 rescues mitotic catastrophe Val
PMID:8573795 rad1-1 H. sapiens RACH2 rescues checkpoint defect, restores UV resistance Val
PMID:8573795 rad1-1/wee1-50 H. sapiens RACH2 rescues lethality Val
PMID:8573795 rad1-1/cdc25-22 H. sapiens RACH2 partially restores the dose-dependent delay in mitotic entry after irradiation Val
PMID:9716408 rad1 (mutant) H. sapiens RAD1 complements UV sensitivity &checkpoint control defect Val
PMID:9828139 rad1 (mutant) H. sapiens RAD1 partially complements UV sensitivity & ionizing radiation sensitivity Val
PMID:8660701 gsa1 M. musculus GSS glutathione synthetase deficiency Val
PMID:8771785 cdc3-124 A. thaliana PFN1 Val
PMID:8771785 cdc3-124 A. thaliana PFN2 Val
PMID:8771785 cdc3-124 A. thaliana PFN3 Val
PMID:8771785 cdc3-124 A. thaliana PFN4 Val
PMID:8943031 rad9-delta H. sapiensRAD9 rescue degrees of sensitivity to hydroxyurea and gamma rays, & associated checkpoint control Val
PMID:9766521 rad9-delta M. musculus Mrad9 restores hydroxyurea resistance and early S phase checkpoint control, UV resistance minimally restored, and mutant cells remain sensitive to gamma radiation Val
PMID:10208430 rad17 H. sapiens hRAD17 partially revert its hydroxyurea and ionizing radiation hypersensitivity, but not its UV hypersensitivity Val
PMID:9013334 ppe1-374 H. sapiens PP6 restores morphology defects Val
PMID:9339378 dis2-11 M. musculus Ppp1cc restores cold sensitivity Val
PMID:9370289 rae1-1 H. sapiens RAE1 partially the temperature sensitivity (by increasing export at restrictive temperature) Val
PMID:9547260 prp8-ts?? H. sapiens DBP2 partially rescued the temperature-sensitive phenotype Val
PMID:9562621 dis3-54 S. cerevisie Dis3 complements Val
PMID:9729469 gpi1 H. sapiens PIGQ rescues lethality Val
PMID:10022880 gap1 ??? D. melanogaster p120 Ras-GAP complement the sterility Val
PMID:10409438 dhm1delta M. musculus dhm1 rescued lethality Val
PMID:7923372 scd1-delta/scd1-1 S. cerevisiae CDC24 partially restores conjugation. Weakly improves cell shape AL
PMID:21449051 arc3-delta H. sapiens ARPC3 ARPC3 restores colony formation of null mutant on solid medium, in liquid cells are very slow growing. Cells are rounder, wider and shorter than normal. Septum is sometimes mispositioned. AL
PMID:11115118krp1-delta S. cerevisiae KEX2 Supports growth at low expression level (i.e. non-induced nmt1 promoter AL
PMID:11115118krp1-delta S. cerevisiae YPS1 Supports growth when overexpressed, like the krp1-ts mutant (i.e. induced nmt1 promoter AL
PMID:10219997gsa1 - allele M379/8 A. thaliana GSH2 cells can grow in presence of 2 mM cadmium. Cells produce similar quantities of GSH but slightly less phytochelatins AL
PMID:8914526gsa1 A. thaliana GSH2 can't access paper on UCL subscription, see if I have it printed at work AL
PMID:9291132 gsa1 - MN101 A. thaliana GSH2 mutant transformed with GSH2 can grow as well as wt in presence of 0.5 mM cadmium but has lower GSH synthase activity than WT: GSH synthetase activity (nmol/h per mg of protein) in WT is 4.8 and in mutant transformed with GSH2 2.5 AL
PMID:7893167 gpt2 CHO (Chinese Hamster Ovary L-G1PT spores are viable when gpt2-delta is complemented by L-G1PT as opposed to null mutant AL
PMID:7499352 tnr3 S. cerevisiase THI80 pho4 is again repressible by thiamine and cells have high TPK activity AL
PMID:2020549 can1 S. cerevisiae CAN1 a can1-1 strain is sensitive to canavanine when grown in minimal media when expressing the CAN1 gene AL
PMID:9404894 sla1 S. cerevisiae LHP1 pombe cells carrying a sla1-deletion form abnormal tRNA intermediates during tRNA maturation (though the mature form appears normal). LHP1 restores the normal intermediate forms AL
PMID:9404894 sla1 H. sapiens SSB pombe cells carrying a sla1-deletion form abnormal tRNA intermediates during tRNA maturation (though the mature form appears normal). SSB restores the normal intermediate forms AL
PMID:18662319 pgt1 S. cerevisiae HGT1 pombe pgt1-delta cells that cannot grow on on GSH as sulphur source, can grow on GSH as sulphur source when expressing cerevisiae HGT1 AL
PMID:18662319 pgt1 S. cerevisiae HGT1 when overexpressing HGT1 cells become sensitive to high doses of GSH AL
PMID:11092853 ppt1 S. cerevisiae COQ2 cells expressing COQ2 have restored coenzyme Q10 production AL
PMID:11071923 int6 human int6 pombe int6-delta grow slower and less than wt in YE and MM. Human int6 rescues growth on plates (liquid not tested) AL
PMID:9368044 crm1 human XPO1 Human XPO1 rescues growth defect at cold temp in the crm1-809 mutant AL
PMID:2112088 ryh1 human Rab6 pombe ryh1-delta doesn't grow at 36 degrees, human rab6 rescues growth defect AL
PMID:3553962 cdc2 human CDK1 pombe cdc2-delta is elongated and growth-arrested. This is rescued by CDC2 AL
PMID:1944283 cdc2 human CDK1 human CDK1 fully rescues pombe cdc2-33 AL
PMID:1435723 byr2 S. cerevisiae STE11 cerevisiae STE11 enables a ste8 mutant to mate and sporulate on sporulation medium. Mat-pm is expressed. AL
PMID:10373512 dss1 H. sapiens SHFM1 human SHFM1 complements growth arrest of pombe dss1 knockout AL
PMID:23447405 ras1 H. sapiens HRAS morphology and pheromone response, tea1 localization and mating partially restored when expressed under control of endogenous promoter AL
PMID:23447405 ras1 H. sapiens KRAS morphology and pheromone response, tea1 localization and mating partially restored when expressed under control of endogenous promoter AL
PMID:23447405 ras1 H. sapiens NRAS mating and tea1 localization partially restored but will only restore morphology and pheromone response partially when overexpressed AL
PMID:12402243 gtb1 S. japonicus gtb1 viability of pombe gtb1-delta mutant (plasmid loss experiment) maintained when japonicus gtb1 is expressed, cells grows as fast as WT AL
PMID:11729194 pnk1 Human PNKP delta-pnk1 cells have no 3'-DNA phosphatase activity. These cells expressing PNKP have 3'-DNA phosphatase activity AL
PMID:11729194 pnk1 Human PNKP delta-pnk1 cells are sensitive to gamma irradiation and camptothecin, PNKP restores normal resistance AL
PMID:22748672 pnk1 cerevisiae TPP1 delta-pnk1 cells are sensitive to gamma irradiation, camptothecin, EMS, MMS, and H2O2. TPP1 restores normal resistance AL
PMID:22692683 ura3 human DHODH ura3-delta uracil auxotrophy rescued by DHODH AL
PMID:7657644 cam1 'vertebrate' vCaM pombe cam1-delta mutant can grow when it is swapped for vertebrate calmodulin AL
PMID:21091378 txl1 h. sapiens TXNL1 pombe cut8-delta and txl1-delta mutant can grow when expressing TXNL1 AL
PMID:11030744 dpm1 t. reesei dpm1 pombe dpm1 deletion mutants do not sporulate, but can when expressing t. reesei dpm1 AL
PMID:11030744 cdc2 G. gallus cdc2 show how pombe cdc2 deletion mutant and cdc2-33 is complemented by gallus gallus cdc2. Also shows effects of mutations in chicken cdc2 on pombe AL
PMID:8021171 tps1 S. cerevisiae TPS1 cerevisiae tps1 enables pombe tps1-delta to make trehalose AL
PMID:11284010 hut1 S. cerevisiae HUT1 cerevisiae hut1 enables pombe hut1-delta to grow on DTT at 37 degrees AL
PMID:6765174 fur4 S. cerevisiae FUR4 cerevisiae fur4 enables pombe fur4 and ura4 mutant to grow on uracil AL

6765174