Changes between Version 14 and Version 15 of GOAnnotationGuidelines

Jan 27, 2015, 5:05:26 PM (6 years ago)



  • GOAnnotationGuidelines

    v14 v15  
    192192 * Also check whether any of these can be experimentally supported by data in the paper
    194 === Updating Existing/Legacy Annotations ===
    195  * Check all existing ISS, IEA, TAS and NAS annotations to see if any are no longer required, or are incorrect
    196   * Remove any TAS/NAS/ISS which are now covered by experiment
    197   * Automated mappings (IEA) will be suppressed by experimental data. Are any IEA annotations not covered by your manual annotation? It should be possible to make a manual annotation to cover all automated mappings (if no experimentally supported annotation can be made, a manually evaluated ISS should be possible)
    198   * Mappings that can't be replaced by manual ISS or experimental annotations may be incorrect and need to be removed. Report incorrect mappings on the [ GO annotation issues tracker]
    199    * Swiss-Prot keyword (SPKW, SP_KW, UniProtKB-KW) mappings: choose category "!UniProt KW2GO mapping", group "GOA", and assign to "goa-ebi"
    200    * Swiss-Prot Subcellular Location (SP_SL, UniProtKB-!SubCell) mappings: category "!UniProt subcell2GO mapping", group "GOA", assign to "goa-ebi"
    201    * !InterPro mappings: category "!InterPro mapping", group "!InterPro", assign to "interhelp"
    202    * For !UniProt keyword and subcellular location mappings, you can also go to the !UniProt entry from a !PomBase gene page and send a message to !UniProt. Ivo Pedruzzi will fix it quickly.
    203   * Some "pombe kw mappings" have NAS evidence code (you will know these are mappings because they will not be visible in the Artemis curation tool. These will need to be deleted from the mapping file.
    204   * If a mapping doesn't seem to be the problem, the ontology may need to be revised; contact GO editors via the [ ontology tracker]
    205  * Check for consistency with other annotations and other resources
    206  * Make sure all remaining ISS are made to an experimentally characterised ortholog.
    207   * If the gene has an S. cerevisiae ortholog, check the annotations to the ortholog in SGD.
    208    * If the gene in SGD is not annotated to a term, and you think it clearly should be, mail them (sgd-helpdesk at to add it so that the ISS is supported. This is frequently required when annotation gene products which are not published.
    209   * Can you make any further annotations based on what SGD has? (Note reasons why annotations cannot be transferred; 1:1 is easiest)