wiki:MidoriCuratedPapers

Version 118 (modified by mah79, 5 years ago) (diff)

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transferred from CuratorTrainingPapers

Quick fixes

Short wait for whatever's needed, so add to existing sessions

Multiple mutations, thorny extensions, etc.

Longer-term, so may require new curation sessions

21813639
mcb1-chk1 genetic interactionalleles OP-mcb1+chk1-deletion, mcb1-D2+chk1-deletion; mcb1-D22+chk1-deletion
mcb1-rad3 genetic interactionalleles OP-mcb1+rad3-deletion, mcb1-D2+rad3-deletion; mcb1-D22+rad3-deletion


20929775
hht1,hht2,hht3 all 3 T3A (triple mutant), sgo2deletiongenetic interaction synthetic lethal
hht1,hht2,hht3, hta1,hta2 all 3 H3 T3A, both H2 S121A (quintuple mutant)genetic interaction synthetic lethal
genetic interactionhrk1, swi6"asynthetic"; check for old same-pathway GO IGI
hht1,hht2,hht3all 3 T3A (triple mutant)genetic interaction "asynthetic" with hrk1deletion
Hrk1-Bir1physical interactionBIR domain of Bir1 (Pfam:PF00653)
hrk1 bub1 genetic interactionalleles hrk1 deletion; bub1-KD (kinase dead, K762R,D900N)
hrk1 sgo2 genetic interactionalleles both deletion
pds5 bub1 genetic interactionalleles pds5 deletion; bub1-KD (kinase dead, K762R,D900N)
pds5 sgo2 genetic interactionalleles both deletion
swi6 sgo2 genetic interactionalleles both deletion


PMID:6887244 long-term note
additional note3 of 13 cold-sensitive mutants curated; if someone can figure out what genes the other 10 correspond to, we can annotate a few more phenotypes


PMID:7865880
other stuffmis2, mis7, mis8, mis9 all apparently uncloned, so don't know which gene they correspond to
mis2 ?geneFYPO:786 ev: plasmid maint assayallele=mis2-87(unknown),condition=PCO:0000005,condition=PCO:0000004 | allele=mis2-197(unknown),condition=PCO:0000004
mis7 ?geneFYPO:786 ev: plasmid maint assayallele=mis7-322(unknown),condition=PCO:0000005
mis8 ?geneFYPO:786 ev: plasmid maint assayallele=mis8-341(unknown),condition=PCO:0000005,condition=PCO:0000004
mis9 ?geneFYPO:786 ev: plasmid maint assayallele=mis9-356(unknown),condition=PCO:0000005


PMID:8631307
cdc21-M68(unknown) mcm4 C-terminus overexpression [note: says overexp piece from BamHI site to end, but the only BamHI site I saw looks too close to N-term to yield fragment in fig 1 gelfypo:2061


PMID:9383050lots of mcm2 alleles done with one on chromosome, another on plasmid (cells otherwise haploid)
mcm2 allele on chromosome/allele on plasmidFYPO ID, high tempFYPO ID, normal temp (32, semi-restrictive)
cdc19-P1/mcm2+fypo:47 normal cell growthfypo:47
cdc19-P1/mcm2-M1fypo:49 inviablefypo:49, fypo:299 microcolony formation, fypo:5 abn. cell morph.
cdc19-P1/mcm2-M2fypo:49fypo:49, fypo:299, fypo:5
cdc19-P1/mcm2-M3fypo:49fypo:49, fypo:299, fypo:5
cdc19-P1/mcm2-M5fypo:49fypo:49, fypo:299, fypo:5
cdc19-P1/mcm2-M6fypo:49fypo:47
cdc19-P1/mcm2-M7fypo:47fypo:46 slow cell growth
cdc19-P1/mcm2-M8fypo:49fypo:46
cdc19-P1/mcm2-M9fypo:49fypo:49, fypo:299, fypo:5
cdc19-P1/mcm2-M10fypo:49fypo:49, fypo:299, fypo:5
cdc19-P1/cdc19-P1fypo:49n/a
cdc19-P1/nda1-KM376fypo:47n/a
cdc19-P1/mcm2-D1fypo:47fypo:47
cdc19-P1/mcm2-D2fypo:47fypo:47
cdc19-P1/mcm2-D3fypo:47fypo:47
cdc19-P1/mcm2-D4fypo:49fypo:49, fypo:299, fypo:5
cdc19-P1/mcm2-D6fypo:49fypo:46
cdc19-P1/mcm2-D7fypo:49fypo:49, fypo:299, fypo:5
cdc19-P1/mcm2-D8fypo:49fypo:46
cdc19-P1/mcm2-D9fypo:49fypo:47
cdc19-P1/mcm2-D10fypo:49fypo:46
wt or deletion setviable at 32dg? (with mcm2 allele of interest overexpressed)
chromosome/plasmidfypo ID, normal temp
mcm2+/mcm2-M1fypo:47
mcm2+/mcm2-M2fypo:47
mcm2+/mcm2-M3fypo:47
mcm2+/mcm2-M5fypo:47
mcm2+/mcm2-M6fypo:47
mcm2+/mcm2-M7fypo:47
mcm2+/mcm2-M8fypo:47
mcm2+/mcm2-M9fypo:47
mcm2+/mcm2-M10fypo:47
mcm2+/mcm2-D1fypo:47
mcm2+/mcm2-D2fypo:47
mcm2+/mcm2-D3fypo:47
mcm2+/mcm2-D4fypo:47
mcm2+/mcm2-D6fypo:47
mcm2+/mcm2-D7fypo:47
mcm2+/mcm2-D8fypo:47
mcm2+/mcm2-D9fypo:47
mcm2+/mcm2-D10fypo:47
mcm2delta/mcm2+fypo:47
mcm2delta/mcm2-M6fypo:49
mcm2delta/mcm2-M7fypo:47
mcm2delta/mcm2-M8fypo:49
mcm2delta/cdc19-P1fypo:47
mcm2delta/mcm2-D1fypo:47
mcm2delta/mcm2-D2fypo:47
mcm2delta/mcm2-D3fypo:47
gene expression complexities
mcm2-M7, D1, D2, D3 and wt alleleslevel of expression from plasmid with nmt promoter much higher with cdc19-P1 on chromosome than with mcm2+ on chr
Mcm2 protein nearly same throughout cell cycle; modest decrease in G2could use artemis gene exp syntax, but don't have a "throughout cell cycle" GO term or extension
ordinary double mutant phenotypes
mcm2 cdc19-P1, mcm4+ overexpslow growth; fypo:150; fypo:614 facsnormal temp
mcm2 cdc19-P1, mcm5+ overexpslow growth; fypo:150; fypo:614 facsnormal temp
mcm2 cdc19-P1, mcm6+ overexpslow growth; fypo:150; fypo:614 facsnormal temp
mcm2 cdc19-P1, cdc6 poldelta-ts1slow cell growth, elong cells, curved cells; normal temp 25 or 29; at 32 dg branched cells, t-shaped cells, inviable high temp (tho 32 is top of PCO "normal" range)
mcm2 cdc19-P1, cdc1-7slow cell growth, elong cells, curved cells; normal temp 25 or 29; at 32 dg branched cells, t-shaped cells, inviable high temp (tho 32 is top of PCO "normal" range)
mcm2 cdc19-P1, cdc18-K46inviable high temp (done at 32, top of PCO "normal" range)


PMID:9658174
annotated physical interactionsno change throughout cell cycle
negative resultno physical interaction between Mcms and Cdc18


PMID:9705504
mcm3-mcm5 phys int "tight"what to do about interaction strength/affinity?


PMID:9914167
mcm2 cdc19-P1FYPO:1386 change 'other' evidence to staining when in ECO
mcm*delta::his3+ (* = 2, 4, 5, or 6)deletion phenotype is that of haploid spore derived from mcm*delta::his3+/mcm*+ diploid; detected protein presumed to be residual from diploid
mcm2delta::his3+/mcm2(cdc19-P1) diploidcouldn't construct; hypothesize that ts mcm2 haploinsufficient
mcm4delta::his3+/mcm4(cdc21-M68) constructed & sporulated at 36dgmcm4delta::his3+ spores: DNA synthesis abolished (didn't enter S phase); 75% abnormal nuclear or cell division


PMID:19168988
note: SPAPB2B4.04c has pmc1 as synonym (used in the paper); documented allele synonyms
multi-way genetic interactionoverexp pmr1+ sup (SPAPB2B4.04cdelta,cta5delta fypo:98)
gene expressionpmr1+ > SPAPB2B4.04c+ > cta5+ during veg growth GO:0072690, but nothing else shown (e.g. change upon stimulus/during GO BP); one measurement


PMID:10409743
hsk1 gene expboth mRNA and protein "relatively constant throughout cell cycle"
dfp1 C-term del, exact residues not specifiedinviable; no further data
Dfp1 "hyperphosphorylated" in presence of HU


PMID:10411894
Dfp1 "hyperphosphorylated" in presence of HUrequires Cds1 kinase


PMID:10490657
lots of Mcm2 & Mcm6 in unspecified "soluble" fractionthroughout cell cycle


PMID:10588642
GFP-beta-galactosidase + SV40 NLSnucleus
GFP-beta-galactosidase + mcm2-M9 residues 5-10 (mutated NLS1)cytoplasm
GFP-beta-galactosidase + Mcm2 residues 114-118 (NLS2)cytoplasm


PMID:11606526
Cdc23 protein loc"insoluble"


PMID:2544292
identified two 5' utrs, starting at -249 and -214; in Artemis they would be:
FT   5'UTR           complement(3175099..3175313)
FT                   /systematic_id="SPBC776.02c"
FT                   /db_xref="PMID:2544292"
FT   5'UTR           complement(3175099..3175348)
FT                   /systematic_id="SPBC776.02c"
FT                   /db_xref="PMID:2544292"
PMID:2544292 cont'dheadmelting genetic interactions (1st 4 phenotypes entered)
cdc25-22 wee1-6 dis2+ overexpts (unlike cdc25 wee1 double mutant)
cdc25-22 wee1-50 dis2+ overexpts
cdc25-22 cdc2-1w dis2+ overexpts
cdc25-22 cdc2-3w dis2+ overexpts
cdc25-r22 sup3-5,r36 ade6-704ade prototroph
cdc25-r22 sup3-5,r36 ade6-704 dis2+ overexpade prototroph
wee 1-6 cdc25-r22 sup3-5,r36 ade6-704ade auxotroph
wee 1-6 cdc25-r22 sup3-5,r36 ade6-704 dis2+ overexpade auxotroph
cdc2-3w cdc25-r22 sup3-5,r36 ade6-704ade auxotroph
cdc2-3w cdc25-r22 sup3-5,r36 ade6-704 dis2+ overexpade auxotroph
relevant background from a cited paper:
sup3-5,r36 ade6-704ade auxotroph
cdc25-rr43 sup3-5,r36 ade6-704ade prototroph
sup3-5,r36 is an opal nonsense suppressor tRNA; ade6-704 has an opal nonsense mutation to suppress


PMID:11973289
mcm4,6,7 alleles all also tested in presence of corresponding ts (mcm4-M68, mcm6-268, mcm7-98) at high temp
mcm6+ with C-terminal 3xHA + mcm6-268 tsinviable if HAtagged not induced, viable if tagged overexp


PMID:12185500
cdc23-M36 mcm2 cdc19-P1inviable st temp 29
cdc23-M36 mcm6 mis5-268inviable st temp 29
cdc23-1E2 mcm2 cdc19-P1inviable st temp 27
cdc23-1E2 mcm6 mis5-268inviable st temp 27
cdc23-M36 mcm4 cdc12-K46inviable st temp 25
cdc23-M36 mcm5 nda4-108inviable st temp 25
cdc23+ overexp, mcm5 nda4-108viable temp 25
gene exp cdc23 mRNA level constant throughout cell cycle


PMID:1849107
cyr1+ overexp, git1-1(unknown)normal glucose repression (i.e. normal neg reg txn by glucose, fypo:1865)
cyr1+ overexp, git3-200(unknown)normal glucose repression
cyr1+ overexp, git5-75(unknown)normal glucose repression
cyr1+ overexp, git7-235(unknown)normal glucose repression
cyr1+ overexp, git8-60(unknown)normal glucose repression
cyr1+ overexp, git10-201(unknown)normal glucose repression
cgs1-1, cyr1-git2-1normal glucose repression
cgs1-1, pka1-git6-261fypo:44 abnormal glucose repression
cyr1/git2 intragenic complementation - groupsgit2-7, git2-110, git2-266, git2-18, git2-68, git2-216, git2-226, git2-210
compl. groupgit2-61, git2-29, git2-83


PMID:1883874
ras1-val17(G17V), gap1+ overexpnormal conjugation micr
sar1 disruption homozygous diploidfypo:279 micr
epistasisste6delta gpa1delta (aka sar1delta) (technically both disruptions) same phenotypes as gpa1delta alone
epistasisras1delta gpa11delta (technically both disruptions) same phenotypes as ras1delta alone


PMID:10655214
orc 2 allele orp2-2, rad1-1cut micr high temp
orc 2 allele orp2-2, rad3deltacut micr high temp
orc2deltafypo:614 (facs; high temp) in germinating orc2delta spores from orp2-2/orc2delta::ura4 diploid (note difference from orc2delta phenotype if diploid was orc2+/orc2delta::ura4
orc2deltacut (micr; high temp) if from from orp2-2/orc2delta::ura4 diploid as above
orc2deltaabnormal repl checkpoint fypo:173 (micr; high temp, +HU) if from from orp2-2/orc2delta::ura4 diploid as above


PMID:10077566
orc4 gene expprotein level constant throughout cell cycle


PMID:2170029
ppa1::ura4+ + ppa2::ura4+inviable
sds21::LEU2 + ppa1::ura4+normal growth normal or high temp
sds21::LEU2 + ppa2::ura4+normal growth normal or high temp
dis2::ura4+ + ppa2::ura4+slow growth (small colonies) normal or high temp
dis2cs R245Q overexp + dis2+ on chrslow growth std temp (restrictive)
dis2cs R245Q overexp + dis2::ura4+ on chrinviable high (permissive) or std temp (restr)
dis2cs R245Q overexp + sds21::LEU2 on chrslow growth std temp (restrictive)
dis2cs R245Q overexp + ppa1::ura4+ on chrslow growth std temp (restrictive)
dis2cs R245Q overexp + ppa2::ura4+ on chrslow growth std temp (restrictive)
dis2 gene expactivity constant throughout cell cycle
ppa2 gene expactivity constant throughout cell cycle


PMID:3185514
ran1/pat1-114 + byr1/ste1-(unknown)same phenotypes as pat1-114 alone - conjugation & sporulation in haploid
ran1/pat1-114 + ste2-(unknown)same phenotypes as pat1-114 alone - conjugation & sporulation in haploid
ran1/pat1-114 + byr1/ste3-(unknown)same phenotypes as pat1-114 alone - conjugation & sporulation in haploid
ran1/pat1-114 + ste4-(unknown)same phenotypes as pat1-114 alone - conjugation & sporulation in haploid
ran1/pat1-114 + ras1/ste5-(unknown)sporulation in haploid as in pat1-114 alone, but no conjugation
ran1/pat1-114 + ste6-(unknown)sporulation in haploid as in pat1-114 alone, but no conjugation
ran1/pat1-114 + ste7-(unknown)same phenotypes as pat1-114 alone - conjugation & sporulation in haploid
ran1/pat1-114 + byr2/ste8-(unknown)same phenotypes as pat1-114 alone - conjugation & sporulation in haploid
ran1/pat1-114 + srw1/ste9-(unknown)same phenotypes as pat1-114 alone - conjugation & sporulation in haploid
ran1/pat1-114 + ste11/aff1-312(unknown)sterile (and not ts as pat1-114 is); abol sporulation


PMID:3442828
win1-1 + cdc25-22 (synonym cdc25.22) + wee1-50 (synonym wee1.50)elongated, minimal med (i.e. more like cdc25-22 alone), normal temp; or rich med, std or high temp
win1-1 + cdc25-22 (synonym cdc25.22) + wee1-50 (synonym wee1.50)normal size (but also has "partially wee") glucose rich med
wee1-50 cdc25-22 win1-1viable rich med, high or std temp, or min med at std temp; inviable minimal med high temp
cf. cdc25-22 + wee1-50wee (as shown previously; presume Ryoko will curate papers)
wee1-50 + win1-1same as win1-1 alone: elongated
cdc25-22 + win1-1more elongated than either single mutant
wee1-6 + win1-1normal size (or very slightly elong) min med std or high temp
wee1-6 + win1-1 cdc25-22mid med: elong std temp; inviable high temp
wee2-3 + win1-1elong min med std or high temp
wee2-3 + win1-1 + cdc25-22elong min med std or high temp


PMID:3448096
cdr2-97 cdc10-129fypo:712 high temp
cdr1-76 cdc10-129fypo:712 high temp
cdr1-76 cdc2-33elongated (lower restrictive temp than cdc2-33 - borderline std/high temp)
cdr2-97 cdc2-33elongated (lower restrictive temp than cdc2-33 - borderline std/high temp)
cdr1-76 cdc25-22elongated, slow growth std temp
cdr2-97 cdc25-22elongated, slow growth std temp
cdr1-76 wee1-50high temp same as wee1-50; std temp larger than wee1-50 but smaller than cdr; high temp elong multiseptate
cdr2-97 wee1-50high temp same as wee1-50; std temp larger than wee1-50 but smaller than cdr; high temp elong multiseptate


PMID:3870979
ran1 pat1-114 in h90 fypo:761 glucose minimal medium std temp (followed by apparently normal sporulation)
pat1-114 in h-normal absence of conjugation minimal? (it's PMA) std temp
pat1-114 in h-normal absence of sporulation PMA std temp
pat1-114 + mei2-16 abol premeiotic dna repl fypo:new1 facs; abol spo micr; high temp
pat1-114 + mei3-17 normal premeiotic dna repl fypo:new2 facs; normal spo micr; high temp
pat1-114 + mei4-2 normal premeiotic dna repl fypo:new2 facs; abol spo micr; high temp
pat1-114 + mei2::LEU2 normal conj micr (h90); abol spo micr (diploid resulting from conj of h90)
ran1::LEU2 + mei2-22viable veg
pat1-114 + mei2-24decr cAMP level std or high temp


PMID:7588609
rep1delta::ura4+ + rep2delta::ura4+abolished sporulation, std temp, micr, high penetrance
rep2delta::ura4+ + res2+ overexpelong veg, std temp, micr; incr duration G1, std temp, facs
rep2delta::ura4+ + res2delta::ura4+normal growth low temp
rep2delta::ura4+ + res1delta::ura4+inviable
epistasisrep2+ overexp no effect on res2delta


PMID:7628434
ssp1-068 ppe1deltafypo:2110 std temp micr
ssp1-068 ppe1deltafypo:1916 high temp micr
ssp1-31 cdc25-22abolished NOTE lower penetrance than ssp1 single mutant (add pen ext to ssp1-11 fypo:1018)


PMID:12597774
hus5+ + hus5.62 overexpdecr sumo fypo:2775 western
hus5+ + hus5.17 overexpnormal sumo fypo:2776 western
hus5.17 + hus5.62less UV sensitive than hus5.62 alone


PMID:12871901
cdt2-M1 cdc25-22cell cycle arrest S phase fypo:611 facs low temp (permissive for cdc25-22; restrictive for cdt2-M1)


PMID:11030343
probably add to phaf instead of session
for X = 16, 17, 25, 37, 46, 47, 51, ts1
cdc18-X rad1-1fypo:650 (micr), fypo:1974 (facs)
cdc18-X hus1-14fypo:650 (micr), fypo:1974 (facs)


PMID:19672306
trr1delta bfr1delta (bfr1 synonym hba2)resistant to caffeine fypo:73 YES expr low
trr1delta bfr1delta caf5deltasensitive to caffeine fypo:97 YES


PMID:11486016
cdc18 overexp + orp2-T4A (orc2 allele)fypo:1425, more severe than cdc18 overexp alone
add new cdc18 annotation, plus double mutant annotation (diff expressivity)


PMID:11689699
all orc subunits - nuclear chromatin go:790 ida (ext throughout cell cycle)


PMID:12186947
orp1-4 rad1 (?allele)cell cycle not arrested as in orp1-4 single mutant; high/restr temp; micr
orp1-4 rad3 (?allele)cell cycle not arrested as in orp1-4 single mutant; high/restr temp; micr
orp1-4 rad17 (?allele)cell cycle not arrested as in orp1-4 single mutant, high/restr temp; micr
orp1-4 rad26 (?allele)cell cycle not arrested as in orp1-4 single mutant; high/restr temp; micr
orp1-4 rad26 (?allele)cut med penetr; high/restr temp; micr
orp1-4 cdc18-K46cut med penetr; high/restr temp; micr
orp1-4 cdc18-K46cut med penetr; high/restr temp; micr
orp1-4 wee1-50cut med penetr; high/restr temp; micr
orp1-4 wee1-50incr level Wee1 protein (and incr Wee1 phosphorylation); high/restr temp; western
orp1-4 chk1deltacut med penetr; high/restr temp; micr
orp1-4 cdc2-1wcut med penetr; high/restr temp; micr
orp1-4 cdc2-3wcut med penetr; high/restr temp; micr
orp1-4 cdc10-129cut med penetr; high/restr temp; micr
phosphorylation of Cdc2 by Wee1 indicates that arrest is due to checkpoint
orc1delta FYPO:0003012 penetrance low if cells exposed to UV


PMID:12181330
alleles
crp79-AB115 del aa 1-92
crp79-AB52 del aa 120-208
crp79-AB12 del aa 209-249
crp79-AB11 del aa 384-432
crp79-AB33 del aa 477-601
crp79-AB58 del aa 601-710
crp79-AB157 del aa 120-382,477-710
crp79-AB177 del aa 209-382,461-710
crp79-AB116 del aa 209-382
crp79-AB178 del aa 209-382,695-710
crp79-AB188 del aa 209-382,478-710 + C-terminal Mex67 NES
crp79-AB179 del aa 209-382,478-710 + C-terminal HIV-Rev NES
crp79-AB180 del aa 209-382,478-710 + C-terminal mutated HIV-Rev NES
multi-gene phenotypes
rae1-167 nup184-1heat sensitive fypo:82
rae1-167 nup184-1fypo:2522 micr high temp
rae1-167 nup184-1 crp79+ overexpnormal pop growth at high temp
rae1-167 nup184-1 crp79+ overexpnormal poly(A)+ mRNA export from nucleus fypo:3027
rae1-167 npp106-1 crp79+ overexpinviable veg pop
rae1-167 crp79+ overexpheat sensitive
rae1-167 nup184-1 crp79-AB3 normal pop growth at high temp
rae1-167 nup184-1 crp79-AB3normal poly(A)+ mRNA export from nucleus fypo:3027
rae1-167 nup184-1 crp79-AB12normal pop growth at high temp
rae1-167 nup184-1 crp79-AB12normal poly(A)+ mRNA export from nucleus fypo:3027
rae1-167 nup184-1 crp79-AB116normal pop growth at high temp
rae1-167 nup184-1 crp79-AB116normal poly(A)+ mRNA export from nucleus fypo:3027
rae1-167 nup184-1 crp79-AB188normal pop growth at high temp
rae1-167 nup184-1 crp79-AB188normal poly(A)+ mRNA export from nucleus fypo:3027
rae1-167 nup184-1 crp79 (crp79-AB52 | crp79-AB11 |crp79-AB33 | crp79-AB58 | crp79-AB157 | crp79-AB177 | crp79-AB178 | crp79-AB177 | crp79-AB179 | crp79-AB180)other crp79 partial deletion mutants do not rescue rae1/nup184 phenotypes, so fypo:82 & fypo:2522 as for double mutant
mex67delta crp79delta::ura4+normal veg pop growth
mex67delta crp79delta::ura4+normal poly(A)+ mRNA export from nucleus fypo:3027
rae1-167 pim1-d1 decr prot export from nucleus fypo:1920 a_u crp79 micr high temp/restr temp


PMID:9671458
note: ran1 allele pat1-114 heat sensitive
pat1-114 nrd1 del aa 2-8 oenormal pop growth high temp (i.e. nrd1 suppresses pat1-114)
pat1-114 nrd1 del aa 2-37 oenormal pop growth high temp (i.e. nrd1 suppresses pat1-114)
pat1-114 nrd1 del aa 2-123 oesensitive to heat (i.e. no suppression)
pat1-114 nrd1 del aa 158-470 oesensitive to heat (i.e. no suppression)
pat1-114 nrd1 del aa 1-301 oesensitive to heat (i.e. no suppression)
pat1-114 nrd1delta cig2+ oenormal pop growth high temp (i.e. cig2 suppresses pat1-114 without nrd1)
pat1-114 nrd1delta pka1+ oenormal pop growth high temp (i.e. pka1 suppresses pat1-114 without nrd1)
pat1-114 pka1delta nrd1+ oenormal pop growth high temp (i.e. nrd1 suppresses pat1-114 without pka1)
pat1-114 cig2delta pka1+ oenormal pop growth high temp (i.e. pka1 suppresses pat1-114 without cig2)
pat1-114 cig2delta nrd1+ oenormal pop growth high temp (i.e. nrd1 suppresses pat1-114 without cig2)
pat1-114 pka1delta cig2+ oesensitive to heat (i.e. no suppression)
wis1delta nrd1deltadecr mating eff micr low glu mm, N absent
wis1delta nrd1deltadecr rna level glu starv a_u ste11 h90 bkg; rna absent a_u ste11 h- bkg; low glu, N absent


PMID:10954610
cds1delta chk1deltanormal mRNA level during UV a_u uvi15 fypo:3035


PMID:22682245
dna2ts cds1deltasame abnormal repl fork reversal as cds1delta single mutant (epistasis); micr (also 2D gel
dna2 S220D cds1deltanormal on HU (sup cds1 single)
dna2ts dna2 E560Asens to heat (implies dna2 E560A alone inviable)
dna2ts dna2 K961Tnormal veg pop growth at high temp (implies dna2 K961T alone viable)


PMID:23260662
need seq feature with ID
mat1-Msmt-0 "deletion of 263 bp distal to mat1"; also see PMID:8431959
mat1-SS2 deletes 111-bp centromere-distal segment 1152-1262 as they've defined cassette (PMID:21423720)fypo:3091 a_u lsd1 chip
double mutants
mat1-Msmt-0 lsd1-E918abol GO:0011000 fypo:3084
lsd1-ao lsd2-aofypo:3081 expr high gel
lsd1-ao lsd2-aofypo:3085 expr med gel
lsd1-ao lsd2-aonormal on TBZ
set1delta clr4deltafypo:3082 gel
clr4delta lsd1-E918fypo:3081 expr high (same as lsd1 single mutant) gel
set1delta lsd1-E918fypo:3081 expr high (same as lsd1 single mutant) gel
set6delta lsd1-E918fypo:3081 expr high (same as lsd1 single mutant) gel
set9delta lsd1-E918fypo:3081 expr high (same as lsd1 single mutant) gel


PMID:12006658
mts4-1 sum1+ overexpelong inviable veg cells std temp expr high


PMID:12514100
swi7-H4 rad17-K118Eincr dur S phase fypo:614 facs std/semi-perm temp expr high
swi7-H4 rad17-K118Emutator fypo:256 cell gr expr low
swi7-H4 rad17-K118Enormal prot autophos fypo:3076 a_u cds1
swi7-H4 rad17-K118Edecr chr bind fypo:2577 a_u kpa1
swi7-H4 cds1-T8Aincr dur S phase fypo:614 facs std/semi-perm temp expr low
swi7-H4 cds1-T8Amutator fypo:256 cell gr expr high
swi7-H4 cds1-T8Anormal prot autophos fypo:3076 a_u cds1
swi7-H4 rad26-T12mutator fypo:256 cell gr expr high
swi7-H4 rev3deltamutator fypo:256 cell gr expr low
swi7-H4 kpa1deltamutator fypo:256 cell gr expr low
swi7-H4 kpa1delta rev3delta|mutatorg fypo:256 cell gr expr low (nearly down to wt)
swi7-H4 rad30deltamutator fypo:256 cell gr expr med
swi7-H4 rad30delta rev3deltamutator fypo:256 cell gr expr med (lower than swi1-H4+rad30delta double mutant)
swi7-H4 rad30delta kpa1deltamutator fypo:256 cell gr expr med (lower than swi1-H4+rad30delta double mutant)


PMID:12615979
double mutants
plo1+ overexp, mad2deltaarrest m phase fypo:608 micr pen low
plo1+ overexp, mad2deltasepta grouped fypo:2002 micr pen med
plo1+ overexp, mad2deltaenlarged nuc fypo:344 micr pen med
plo1-DHK625AAA overexp, mad2deltasepta grouped fypo:2002 micr pen med
plo1-K69R overexp, mad2deltaenlarged nuc fypo:344 micr pen med
plo1-K69R overexp, plo1-YQL508AAA overexpnormal veg cell morph micr


PMID:17085965
tea1delta rax2deltafypo:3314 micr expr high
tea1delta rax2deltafypo:1019 micr
cdc10-129 rax2deltafypo:3314 micr expr med


PMID:16096637
cdc10-129 pmo25-35fypo:2187 micr high temp
cdc25-22 pmo25-35fypo:3333, fypo:2021 both micr high temp
cdc10-129 nak1-125fypo:2187 micr high temp
cdc25-22 nak1-125fypo:2021 micr high temp
cdc10-129 mor2-786fypo:2187 micr high temp
cdc25-22 mor2-786fypo:2021 micr high temp


PMID:16325501
pmo25 protein levelconstant throughout cell cycle
cdc25-22 pmo25-2fypo:3333 micr high temp
orb6-25 pmo25-2fypo:2187 micr high temp


PMID:16772338
pending outcome of chado SF 327could add myp2 protein binding go:5515 ipi with chs2 (if it won't be inferred somewhere)
myo2-E1 myp2deltaabol prot loc contr ring fypo:2561 a_u chs2 high temp pen full
myo2-E1 myp2deltanormal veg pop gr high temp
chs2delta cdc15-40inviable veg pop std temp
chs2delta cdc4-8inviable veg pop std temp
chs2delta imp2deltanormal veg pop gr high temp
chs2delta imp2deltafypo:1492 micr high temp
chs2delta myo2-E1 myp2deltainviable veg pop high temp
chs2delta myp2deltafypo:1214 std temp expr high
chs2delta myp2deltafypo:1253 high temp pen high
chs2delta myp2deltafypo:1406 high temp pen med
chs2delta myp2deltafypo:2028 micr high temp pen low
chs2delta myp2deltaabn actomyosin contr ring morphology fypo:3338
chs2delta myp2deltafypo:1365 expr high
chs2delta myp2deltafypo:3014 expr high
chs2delta myp2deltadecr rate ring assembly fypo:3339 expr med
chs2delta myo2-E1incr sept index fypo:650 expr high std temp


PMID:16824200
nda3-KM311 mad2deltaabol loc to spindle midzone fypo:3350 a_u bir2; micr; low/restrictive temp
nda3-KM311 mad2deltaMTs absent fypo:2004 micr; low/restrictive temp
nda3-KM311 mad2deltaabn chr seg fypo:141; micr; low/restrictive temp


PMID:18256290
pending outcome of chado SF 327could add rho1 protein binding go:5515 ipi with pxl1 (if it won't be inferred somewhere)
double mutantsin some cases "high temp" is a fudge for higher vs. lower within PECO "standard" range
cdc42-1695 pxl1+ overexpinv pear fypo:2904 high temp
cdc42-1695 pxl1+ overexpnormal pop growth high temp fypo:1357
rga5delta pxl1deltaincr septation index fypo:650 std temp expr med (increased more than pxl1delta alone, but probably still in "medium" range)
pxl1delta rgf3+ overexpincr sept index fypo:650 std temp expr med
pxl1delta rgf3+ overexpfypo:1253 std temp expr med
pxl1delta ehs2-1 (rgf3 allele)decr veg pop growth fypo:1355 high temp expr low
pxl1delta ehs2-1 (rgf3 allele)normal on NaF fypo:3370
pxl1delta mid1deltainviable veg pop std temp; slow fypo:1234 high temp
pxl1delta rlc1deltainviable veg pop std temp; slow fypo:1234 high temp
pxl1delta myo2-E1inviable veg pop high temp; slow fypo:1234 std temp
pxl1delta cdc4-8inviable veg pop std temp
pxl1delta cdc4-8inviable branched multisept fypo:3413
pxl1delta rng2-D5inviable veg pop std temp
pxl1delta rng2-D5inviable branched multisept fypo:3413
pxl1delta cdc15-140inviable veg pop high temp
pxl1delta bgs1-cps1-N12inviable veg pop std temp
pxl1delta bgs1-cps1-12inviable veg pop std temp
pxl1delta bgs1-cps1-191inviable veg pop std temp
pxl1delta cdc12deltaincr septation index fypo:650 std temp expr med (increased more than pxl1delta alone, but probably still in "medium" range)
pxl1delta myo3deltaincr septation index fypo:650 std temp expr med (increased more than pxl1delta alone, but probably still in "medium" range)
pxl1delta mid2deltaincr septation index fypo:650 std temp expr med (increased more than pxl1delta alone, but probably still in "medium" range)
pxl1delta spn3deltaincr septation index fypo:650 std temp expr med (increased more than pxl1delta alone, but probably still in "medium" range)


PMID:19217403
if GO ever gets round to sorting out the "localized to bit of chromosome containing SO:nnn sequence feature", could add extension to GO:0000780at SO:0001796 & SO:0001798
scm3-6 cnp1+ overexpviable veg pop high temp
scm3-19 cnp1+ overexpviable veg pop high temp
scm3-6 mis6-302inviable veg pop std temp
scm3-19 mis6-302inviable veg pop std temp
scm3-6 mis16-53inviable veg pop std temp
scm3-19 mis16-53inviable veg pop std temp
scm3-19 mis18-262decr veg pop growth std temp expr med
scm3+ overexp cnp1-1sens heat; slow veg pop growth std temp
scm3+ overexp mis6-302sens heat; inviable veg pop std temp
scm3+ overexp mis12-537sens heat
scm3+ overexp mis14-271sens heat
scm3+ overexp mis15-68sens heat; inviable veg pop std temp
scm3+ overexp mis16-53sens heat; slow veg pop growth std temp
scm3+ overexp mis17-362sens heat; slow veg pop growth std temp
scm3+ overexp mis18-262sens heat; slow veg pop growth std temp


PMID:8041618
srp7-A154U srp54+ overexpfypo:2060 high temp, yes+glycerol
srp7-TS-point (G107A,G112A) srp54+ overexpfypo:2060 high temp, yes
srp7-G4C srp54+ overexpfypo:81 expr low, high temp, yes+glycerol


PMID:7969124
srp54 allele combinationswt and mutant alleles on low-copy plasmids (cells otherwise haploid)
srp54+/T248Ndecr veg gr fypo:1355 min med; expr low at low temp (growth normal at std or high temp)
srp54+/R194Ldecr veg gr fypo:1355 min med; expr low at low or std temp; expr high at high temp
srp54+/K114Rdecr veg gr fypo:1355 min med; expr low; expr high at high temp
srp54 allele combinationsdiploids, wt on one chromosome, mutant on high-copy plasmid
srp54+/T248S decr veg gr fypo:1355 min med; expr low at low temp (growth normal at std or high temp)
srp54+/T248K decr veg gr fypo:1355 min med; expr high at low temp, expr low at high temp (growth normal at std temp)
srp54+/T248R decr veg gr fypo:1355 min med; expr high at low temp, expr low at high temp (growth normal at std temp)
srp54+/T248Ndecr veg gr fypo:1355 min med; expr low at high or low temp (growth normal at std temp)
srp54+/R194Q decr veg gr fypo:1355 min med; expr high at high or low temp (growth normal at std temp)
srp54+/R194L decr veg gr fypo:1355 min med; expr high at high or low temp (growth normal at std temp)
srp54+/R194H decr veg gr fypo:1355 min med; expr low at low temp, expr high at high temp (growth normal at std temp)
srp54+/D190Ndecr veg gr fypo:1355 min med; expr low at low temp (growth normal at std or high temp)
srp54+/D138Ndecr veg gr fypo:1355 min med; expr low at low temp, expr high at high temp (growth normal at std temp)
srp54+/K114R decr veg gr fypo:1355 min med; expr high at high or low temp (growth normal at std temp)
srp54+/K114G decr veg gr fypo:1355 min med; expr low at low temp, expr high at high temp (growth normal at std temp)
srp54+/Q110G decr veg gr fypo:1355 min med; expr high at low temp, expr low at high temp (growth normal at std temp)
srp54+/Q110E decr veg gr fypo:1355 min med; expr high at high temp (growth normal at std or low temp)
srp54+/Q110R decr veg gr fypo:1355 min med; expr low at high or low temp (growth normal at std temp)
srp54+/Q110Kdecr veg gr fypo:1355 min med; expr low at high or low temp (growth normal at std temp)


PMID:10079327
srp54 allele combinationswt and mutant alleles on low-copy plasmids (cells otherwise haploid)
srp54+/R194A,H195Adecr veg gr fypo:1355 min med; expr low at low temp, expr high at high temp (growth normal at std temp)
srp54+/E277A,H278Adecr veg gr fypo:1355 min med; expr low at high temp (growth normal at std or low temp)
srp54+/K314A,K315Adecr veg gr fypo:1355 min med; expr low at high temp (growth normal at std or low temp)
srp54+/K428A,K429Adecr veg gr fypo:1355 min med; expr low at high temp (growth normal at std or low temp)
srp54 allele combinationsdiploids, wt on one chromosome, mutant on high-copy plasmid
srp54+/R194A,H195Adecr veg gr fypo:1355 min med; expr high at high or low temp (growth normal at std temp)
srp54+/E277A,H278Adecr veg gr fypo:1355 min med; expr high at high temp (growth normal at std or low temp)
srp54+/K314A,K315Adecr veg gr fypo:1355 min med; expr high at high temp (growth normal at std or low temp)
srp54+/K428A,K429Adecr veg gr fypo:1355 min med; expr high at high temp (growth normal at std or low temp)


PMID:7700230
chk1delta cdc6-ts1loss of viability at high temp fypo:1387 expr high YEA agar
chk1delta cdc6-ts2fypo:1387 expr high YEA agar
chk1delta cdc6-ts3fypo:1387 expr high YEA agar
chk1delta cdc20-M10fypo:1387 expr low YEA agar
chk1delta cdc18-K46fypo:1387 expr high YEA agar
chk1delta cdc18-K46fypo:1387 expr high YEA liquid
chk1delta cdc6-ts1mitotic catastrophe with cut, normal size cell fypo:1053 high temp
chk1delta cdc6-ts2fypo:1053 high temp
chk1delta cdc6-ts3fypo:1053 high temp
chk1delta cdc20-M10fypo:1053 high temp
chk1delta cdc18-K46fypo:1053 high temp
chk1delta cdc18-K46fypo:1053 high temp
chk1delta cdc6-ts1DNA content decreased during veg fypo:453 or add child for "less than 1C" facs high temp
chk1delta cdc6-ts2fypo:453 or add child for "less than 1C" facs high temp
chk1delta cdc6-ts3fypo:453 or add child for "less than 1C" facs high temp
chk1delta cdc20-M10fypo:453 or add child for "less than 1C" facs high temp
chk1delta cdc18-K46fypo:453 or add child for "less than 1C" facs high temp
chk1delta cdc18-K46fypo:453 or add child for "less than 1C" facs high temp


Stored additional specificity for evidence (in case we ever want to go back and "upgrade")

PMID:7865880mis5-268(unknown), PCO:0000004FYPO:217PFGE
PMID:8895665orc1 cdc30-4(unknown)FYPO:0000217PFGE
PMID:8838655mcm2FYPO:0000217PFGE
PMID:8838655mcm2FYPO:0001133PFGE
PMID:8838655mcm5FYPO:0000217PFGE
PMID:8838655mcm5FYPO:0001133PFGE
PMID:9914167mcm2 cdc19-P1FYPO:848PFGE
PMID:11606526mcm7-98FYPO:0000848PFGE
PMID:7628438ssp1 allelesFYPO: normal chr morphPFGE

Community Curation sessions I've reviewed

PMID:22768263original session c9cf7fe37e5e89fa
sec3-2, exo70deltaabolished prot loc to cell tip fypo:1585; high temp; annotation_extension=assayed_using(PomBase:SPCC622.10c),annotation_extension=assayed_using(PomBase:SPAC6F12.08c)


PMID:23677513 fdeaa8d994824f8c
double mutant sensitivities to bleomycin, phleomycin, cisplatin, oxaliplatincell growth assay; synthetic complete medium
myh1delta rad1delta FYPO:0000095, FYPO:0000102, FYPO:0002344, FYPO:0002345
myh1delta hus1delta FYPO:0000095, FYPO:0000102, FYPO:0002344, FYPO:0002345; but same as hus1delta alone
myh1delta rad9delta FYPO:0000095, FYPO:0000102, FYPO:0002344, FYPO:0002345; but same as rad9delta alone
can also add myh1 chromatin binding in_presence_of phleomycin when ChEBI adds phleomycin (https://sourceforge.net/p/chebi/curator-requests/2155/)


PMID:22683269 5bf20875f2c283f7
clr4delta cid14deltaincreased level of heterochromatin-encoded proteins (western)
swi6delta cid14deltaincreased level of heterochromatin-encoded proteins (western)
swi6delta cid14deltadecreased H3K9me2 at silent mating (ChIP)
swi6delta cid14deltanormal H3K9me2 at telomere (ChIP)


PMID:22918952
chk1delta cds1deltafypo:2535 fypo:2536
rad3delta pmk1deltafypo:2535 fypo:2536
rad3delta cdc2(cdk1-as)fypo:2534 fypo:2536 +1NM-PP1
pmk1delta cdc2(cdk1-as)fypo:2534 +1NM-PP1
rad3delta pmk1delta cdc2(cdk1-as)fypo:2534 +1NM-PP1
pmk1delta cdc2(cdk1-as)fypo:2539 +1NM-PP1
rad3delta pmk1delta cdc2(cdk1-as)fypo:2538 +1NM-PP1
rad3delta pmk1delta cdc2(cdk1-as)fypo:2542 fypo:2543 fypo:2544 fypo:2545 +1NM-PP1
all get annotation_extension=assayed_using(PomBase:SPAC1782.09c) clp1 microscopy if any of fypo:2534-2541; western if any of fypo:2542-2545


PMID:23091701
ppc1-537 mst1-L344Sinviable veg pop std temp
ppc1-537 clr6-1inviable veg pop std temp


PMID:22683458
dsk1NS kic1deltanormal cell pop growth
dsk1K110A kic1deltainviable veg cell pop


PMID:23041194
scs2delta scs22deltaabnormal ER localization fypo:2872 micr expr high
scs2delta scs22delta for3deltastubby cell; abnormal ER localization fypo:2872 micr
scs2delta scs22delta for3delta myo51delta myo52deltastubby cell; abnormal ER localization fypo:2872 micr
tts1delta rtn1delta yop1deltamisoriented septum
tts1delta rtn1delta yop1deltaabol prot loc med cortex fypo:1371 a_u mid2
tts1delta rtn1delta yop1delta scs2delta scs22deltanormal septum orientation fypo:2873
scs2delta scs22delta advanced prot loc med cortex fypo:2874 a_u mid2
scs2delta scs22delta tts1delta rtn1delta yop1deltanormal prot loc med cortex fypo:2558 a_u mid2


PMID:23089178
asf1-33 sim3 overexpnormal chromatin org fypo:2891 electroph
asf1-33 sim3 overexpnormal heterochromatin org fypo:2892 cell growth
asf1-33 sim3deltainviable veg cell pop


PMID:22496451
ppd1delta prz1 overexpsame increased txn & slow growth as prz1 overexp alone
prz1 overexp + rad24 overexpnormal veg cell pop growth
prz1 overexp + rad25 overexpnormal veg cell morph (same cond as single mutant); normal veg cell pop growth
prz1 overexp + pac1 overexpnormal veg cell morph (same cond as single mutant)
prz1 overexp + ape2 overexpspheroid fypo:21, same cond but lower expressivity (use med fypo_ext:2)
prz1 overexp + tfs1 overexpspheroid fypo:21, same cond but lower expressivity (use med fypo_ext:2)
prz1 overexp + pka1 overexpelongated branched veg cell
prz1 overexp + msn5 overexpelongated branched veg cell
rad24delta + rad25 overexpnormal on tacrolimus fypo:1470


PMID:23211746
chk1-E472D ast1 overexpnormal on MMS high temp
chk1-E472D ast1 overexpnormal prot phos during dna damage fypo:2899
chk1-E472D ast1ND overexpsens to MMS high temp (ast1ND = D158A, D160A, D179A, D181A)
chk1-D155A ast1 overexpsens to MMS std temp
chk1delta ast1deltasens to MMS std temp (same as chk1delta alone
chk1delta ast1deltasens to UV std temp (same as chk1delta alone
rad2delta exo1deltainviable elong veg cells (note: viable at 2 days, but die later)
rad2delta exo1delta chk1deltainviable elong veg cells
rad2delta exo1delta chk1deltacut
rad2delta exo1delta ast1deltacut
rad2delta exo1delta ast1deltainviable microcolony elong
exo1delta ast1deltasame MMS sens, same Chk1 phosphorylation as exo1delta alone
rad2delta ast1deltasame MMS sens, same Chk1 phosphorylation as rad2delta alone
exo1-so rad2deltainviable veg pop
exo1-so rad2delta ast1deltainviable veg pop
exo1-so rad2deltafypo:12
exo1-so rad2deltaabnormal DNA damage checkpoint during cellular response to UV fypo:2900
exo1-so rad2delta ast1deltafypo:2102
exo1-so rad2delta ast1deltafypo:141 expr low
exo1-so rad2delta chk1deltafypo:141 expr high
exo1-so rad2delta chk1-S345Afypo:141 expr med
exo1-so rad2deltaabol prot phos DNA damage fypo:2898 a_u chk1
exo1-so rad2delta ast1deltadecr prot phos DNA damage fypo:2897 a_u chk1
ast1delta rad32deltasens to MMS
ast1delta rad32delta pku70deltanormal on MMS
exo1delta rad32deltasens to MMS
exo1delta rad32delta pku70deltasens to MMS
exo1-so rad2deltafypo:2474 a_u RPA, rad51
exo1-so rad2delta ast1deltafypo:2474 a_u RPA, rad51
exo1-so rad2delta ast1deltafypo:2553 (could add decreased GO:0010792 if they want it that specific) southern blot


PMID:23427262
ndc80-NH12 mad2deltafypo:168?
update fypo:1645 extension assayed_using(ndc80, alp7)
Nuf2-Alp7C fusion + ndc80-NH12(nearly) normal veg cell pop growth; normal chromosome seg (fig 6)
ndc80-NH12 + dis1deltainviable veg cell pop
ndc80-NH12/ndc80-21 heterozygous diploidsensitive to high temp, but not as severe as either homozygous diploid (fig 7)


PMID:23576550
update fypo:1571 extension assayed_using(alp41, alp1)
tbc1-11 alp41-8sens to high temp
tbc1-11 alp41-14normal veg pop growth high temp


PMID:24081329
nab2::kanMX6 pab1::ura4+decr veg cell pop growth std temp & high temp
nab2::kanMX6 pab1::ura4+incr polyA tail length fypo:2931 gel (more severe than pab1- alone, so expr high)
nab2::kanMX6 pab1::ura4+incr pre-mRNA level fypo:1908 northern (more severe than pab1- alone, so expr high) a_u rpl3002
nab2::kanMX6 pab1::ura4+incr mRNA level fypo:2936 northern (more severe than pab1- alone, so expr high) a_u rpl3002
nab2::kanMX6 rpl3002deltainormal mRNA level fypo:2938 a_u rpl3002
nab2::kanMX6 rpl3001::kanMX6same pre-mRNA level as rpl3001 single mutant a_u rpl3002 (epistasis)
nab2::kanMX6 prp1-4less excess pre-mRNA than prp1-4 alone (incr pre-mRNA level fypo:1908 northern expr low) high temp a_u rpl3002
nab2::kanMX6 prp2-1less excess pre-mRNA than prp2-1 alone (incr pre-mRNA level fypo:1908 northern expr low) high temp a_u rpl3002
nab2::kanMX6 rrp6::ura4+same pre-mRNA & mRNA levels as rrp6- alone (epistasis) a_u rpl3002


PMID:23874188credit Jun-Song Chen where noted (MAH did growth phenotypes; J-S C did microscopy-based
cdc11-S5A cdc16-116sens to heat expr high YES
cdc11-S8A cdc16-116sens to heat expr low YES
cdc16-116 csc1::ura4sens to heat expr med YES
cdc11-S5A cdc16-116 csc1::ura4sens to heat expr high YES
cdc11-S8A cdc16-116 csc1::ura4sens to heat expr low YES
cdc11-S5A cdc16-116 csc1::ura4multiseptate expr med (40%) micr; J-S C
cdc11-S5A cdc16-116 csc1::ura4cut expr low (15%) micr; J-S C


PMID:24146635
mas5delta cdc42-G12V overexpfypo:3150 expr low
mas5delta cdc42-G12V overexpnormal monopolar cell growth fypo:3156 penetr med
mas5delta cdc42-G12V overexpintermittent fypo:3155 penetr low
mas5delta hsf1+ knockdownincr prot lev fypo:1327 a_u hsp104 std temp expr low
mas5delta hsf1+ knockdownfypo:3150 expr low
mas5delta hsf1+ knockdownnormal monopolar cell growth fypo:3156 penetr med
mas5delta hsf1+ knockdownintermittent fypo:3155 penetr low


PMID:24316795
pom1delta305N bud6deltafypo:2253, fypo:648, fypo:2258 a_u cdr2 (same as pom1 single mutant) micr emm std temp
pom1+/pom1delta diploidfypo:648 micr emm std temp
pom1+/pom1delta diploidfypo:2558 a_u cdr2 micr emm std temp
pom1+/pom1delta diploidfypo:2253 micr emm std temp
pom1+/pom1delta diploiddecr prot lev fypo:1324 a_u pom1 expr med micr emm std temp
cdr2-GFP/cdr2deltafypo:1124 micr emm std temp
pom1delta pnmt81-cdr2fypo:648 micr expr high
pom1delta pnmt81-cdr2-mEGFPfypo:648 micr expr high
pom1delta cdr2-S755Afypo:648 expr med (slightly shorter than cdr2 single mutant) micr
cdr2-S755A pom1deltafypo:339 micr
cdr2-S755A-758A pom1deltafypo:339 micr
cdr2-S755A-758A pom1deltafypo:2556 a_u cdr2
cdr2-S755A-758A + (pom1delta or pom1delta305N)same cell length as cdr2-S755A-758A alone


PMID:23394829
mad2+ overexp nak1-167premature septation fypo:3250 micr expr high
mad2+ overexp sid1-250normal septation (i.e. no premature septation)
mad2+ overexp nak1-167 sid1-250normal septation (i.e. no premature septation)
nak1-5A cdc3-124 cdc16-116abol prot phos fypo:2033 enz assay a_u nak1
cdc3-124 cdc16-116normal prot phos fypo:776 enz assay a_u nak1
nak1-5A cdc3-124 cdc16-116viable elong veg fypo:1492 micr high temp
nak1-5E fypo:705 when canto SF 345 doneupdate to a_u nak1,mob2


PMID:24210919
rrp6delta rmn1deltafypo:3231 chip
rrp6delta iss10deltafypo:3234 chip
rrp6delta nrl1deltafypo:3103 rnaseq
rrp6delta nrl1deltafypo:3231 chip
rrp6delta nrl1deltafypo:3236 rnaseq
rrp6delta cwf10-1fypo:3103 rnaseq
rrp6delta cwf10-1fypo:3231 chip
rrp6delta cwf10-1fypo:3236 rnaseq


PMID:24344203
tsc1delta sin1deltafypo:250
tsc2delta sin1deltafypo:250
tsc1delta gad8deltafypo:250
tsc2delta dag8deltafypo:250
tsc1delta tor1SE endogfypo:111 cond peco:246
tsc1delta tor2SE endogfypo:2672 cond peco:246
tsc1delta isp7+ overexpfypo:2672 cond peco:246
tsc2delta isp7+ overexpfypo:2672 cond peco:246
tsc1delta isp7deltafypo:3252
tsc2delta isp7deltafypo:3252
tsc2delta isp7deltafypo:3254
tsc2delta isp7deltafypo:35
tor2-51 isp7deltafypo:1592 expr high
tor2-51 isp7deltafypo:3258 expr med std temp
tor2-51 isp7deltafypo:new6 high/restr temp
tsc2delta isp7deltafypo:3255
tor1delta isp7deltafypo:3257 expr med
tsc2delta isp7+ overexpfypo:3255
tor2SE isp7+ overexpfypo:1838 cond +rapa a_u rps602
gtr1delta tsc2deltafypo:3252
gtr2delta tsc2deltafypo:3252
gtr1delta isp7deltafypo:243
gtr2delta isp7deltafypo:243
tsc2delta isp7+ overexpfypo:1038 a_u rps602 (same as isp7+ oe alone)
tsc2delta isp7-H276Afypo:1038 a_u rps602 (same as isp7+ oe alone)
tsc2delta isp7-H276Afypo:111


PMID:23297348
clp1delta csc1deltafypo:1253 micr expr high
cdc11-S8A sid1-239sens heat; decr veg pop gr YE std temp expr low (same as sid1-239 alone)
cdc11-S8D sid1-239decr veg pop gr YE std temp expr med
cdc11-S8A spg1-106decr veg pop gr YE std temp expr med (same as spg1-106 alone)
cdc11-S8D spg1-106decr veg pop gr YE std temp expr high
cdc11-S8A cdc7-24sens heat expr high (same as cdc7-24 alone)
cdc11-S8D cdc7-24sens heat expr high (same as cdc7-24 alone)


PMID:23348717
AspRec8c–FGFP overexp ubr11deltadecr veg pop growth expr high
AspRec8c–FGFP overexp ubr11deltaincr minichr loss


PMID:23671279
gcn2delta cpc2deltasens amitrole fypo:1097 min med (same as either single mutant)
hri2delta cpc2deltasens amitrole fypo:1097 min med (same as cpc2delta alone)
gcn2delta cpc2deltadecr prot phos fypo:1838 min med amitrole a_u tif211 expr med (same as cpc2delta alone)
hri2delta gcn2deltaabol prot phos fypo:2033 min med amitrole a_u tif211 (same as gcn2delta alone)
hri2delta cpc2deltadecr prot phos fypo:1838 min med amitrole a_u tif211 expr high (worse than cpc2delta alone)