wiki:MidoriCuratedPapers

Version 127 (modified by mah79, 6 years ago) (diff)

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transferred from CuratorTrainingPapers

Quick fixes

Short wait for whatever's needed, so add to existing sessions

Multiple mutations, thorny extensions, etc.

Longer-term, so may require new curation sessions

21813639
mcb1-chk1 genetic interactionalleles OP-mcb1+chk1-deletion, mcb1-D2+chk1-deletion; mcb1-D22+chk1-deletion
mcb1-rad3 genetic interactionalleles OP-mcb1+rad3-deletion, mcb1-D2+rad3-deletion; mcb1-D22+rad3-deletion


20929775
hht1,hht2,hht3 all 3 T3A (triple mutant), sgo2deletiongenetic interaction synthetic lethal
hht1,hht2,hht3, hta1,hta2 all 3 H3 T3A, both H2 S121A (quintuple mutant)genetic interaction synthetic lethal
genetic interactionhrk1, swi6"asynthetic"; check for old same-pathway GO IGI
hht1,hht2,hht3all 3 T3A (triple mutant)genetic interaction "asynthetic" with hrk1deletion
Hrk1-Bir1physical interactionBIR domain of Bir1 (Pfam:PF00653)
hrk1 bub1 genetic interactionalleles hrk1 deletion; bub1-KD (kinase dead, K762R,D900N)
hrk1 sgo2 genetic interactionalleles both deletion
pds5 bub1 genetic interactionalleles pds5 deletion; bub1-KD (kinase dead, K762R,D900N)
pds5 sgo2 genetic interactionalleles both deletion
swi6 sgo2 genetic interactionalleles both deletion


PMID:6887244 long-term note
additional note3 of 13 cold-sensitive mutants curated; if someone can figure out what genes the other 10 correspond to, we can annotate a few more phenotypes


PMID:7865880
other stuffmis2, mis7, mis8, mis9 all apparently uncloned, so don't know which gene they correspond to
mis2 ?geneFYPO:786 ev: plasmid maint assayallele=mis2-87(unknown),condition=PCO:0000005,condition=PCO:0000004 | allele=mis2-197(unknown),condition=PCO:0000004
mis7 ?geneFYPO:786 ev: plasmid maint assayallele=mis7-322(unknown),condition=PCO:0000005
mis8 ?geneFYPO:786 ev: plasmid maint assayallele=mis8-341(unknown),condition=PCO:0000005,condition=PCO:0000004
mis9 ?geneFYPO:786 ev: plasmid maint assayallele=mis9-356(unknown),condition=PCO:0000005


PMID:8631307
cdc21-M68(unknown) mcm4 C-terminus overexpression [note: says overexp piece from BamHI site to end, but the only BamHI site I saw looks too close to N-term to yield fragment in fig 1 gelfypo:2061


PMID:9383050lots of mcm2 alleles done with one on chromosome, another on plasmid (cells otherwise haploid)
mcm2 allele on chromosome/allele on plasmidFYPO ID, high tempFYPO ID, normal temp (32, semi-restrictive)
cdc19-P1/mcm2+fypo:47 normal cell growthfypo:47
cdc19-P1/mcm2-M1fypo:49 inviablefypo:49, fypo:299 microcolony formation, fypo:5 abn. cell morph.
cdc19-P1/mcm2-M2fypo:49fypo:49, fypo:299, fypo:5
cdc19-P1/mcm2-M3fypo:49fypo:49, fypo:299, fypo:5
cdc19-P1/mcm2-M5fypo:49fypo:49, fypo:299, fypo:5
cdc19-P1/mcm2-M6fypo:49fypo:47
cdc19-P1/mcm2-M7fypo:47fypo:46 slow cell growth
cdc19-P1/mcm2-M8fypo:49fypo:46
cdc19-P1/mcm2-M9fypo:49fypo:49, fypo:299, fypo:5
cdc19-P1/mcm2-M10fypo:49fypo:49, fypo:299, fypo:5
cdc19-P1/cdc19-P1fypo:49n/a
cdc19-P1/nda1-KM376fypo:47n/a
cdc19-P1/mcm2-D1fypo:47fypo:47
cdc19-P1/mcm2-D2fypo:47fypo:47
cdc19-P1/mcm2-D3fypo:47fypo:47
cdc19-P1/mcm2-D4fypo:49fypo:49, fypo:299, fypo:5
cdc19-P1/mcm2-D6fypo:49fypo:46
cdc19-P1/mcm2-D7fypo:49fypo:49, fypo:299, fypo:5
cdc19-P1/mcm2-D8fypo:49fypo:46
cdc19-P1/mcm2-D9fypo:49fypo:47
cdc19-P1/mcm2-D10fypo:49fypo:46
wt or deletion setviable at 32dg? (with mcm2 allele of interest overexpressed)
chromosome/plasmidfypo ID, normal temp
mcm2+/mcm2-M1fypo:47
mcm2+/mcm2-M2fypo:47
mcm2+/mcm2-M3fypo:47
mcm2+/mcm2-M5fypo:47
mcm2+/mcm2-M6fypo:47
mcm2+/mcm2-M7fypo:47
mcm2+/mcm2-M8fypo:47
mcm2+/mcm2-M9fypo:47
mcm2+/mcm2-M10fypo:47
mcm2+/mcm2-D1fypo:47
mcm2+/mcm2-D2fypo:47
mcm2+/mcm2-D3fypo:47
mcm2+/mcm2-D4fypo:47
mcm2+/mcm2-D6fypo:47
mcm2+/mcm2-D7fypo:47
mcm2+/mcm2-D8fypo:47
mcm2+/mcm2-D9fypo:47
mcm2+/mcm2-D10fypo:47
mcm2delta/mcm2+fypo:47
mcm2delta/mcm2-M6fypo:49
mcm2delta/mcm2-M7fypo:47
mcm2delta/mcm2-M8fypo:49
mcm2delta/cdc19-P1fypo:47
mcm2delta/mcm2-D1fypo:47
mcm2delta/mcm2-D2fypo:47
mcm2delta/mcm2-D3fypo:47
gene expression complexities
mcm2-M7, D1, D2, D3 and wt alleleslevel of expression from plasmid with nmt promoter much higher with cdc19-P1 on chromosome than with mcm2+ on chr
Mcm2 protein nearly same throughout cell cycle; modest decrease in G2could use artemis gene exp syntax, but don't have a "throughout cell cycle" GO term or extension
ordinary double mutant phenotypes
mcm2 cdc19-P1, mcm4+ overexpslow growth; fypo:150; fypo:614 facsnormal temp
mcm2 cdc19-P1, mcm5+ overexpslow growth; fypo:150; fypo:614 facsnormal temp
mcm2 cdc19-P1, mcm6+ overexpslow growth; fypo:150; fypo:614 facsnormal temp
mcm2 cdc19-P1, cdc6 poldelta-ts1slow cell growth, elong cells, curved cells; normal temp 25 or 29; at 32 dg branched cells, t-shaped cells, inviable high temp (tho 32 is top of PCO "normal" range)
mcm2 cdc19-P1, cdc1-7slow cell growth, elong cells, curved cells; normal temp 25 or 29; at 32 dg branched cells, t-shaped cells, inviable high temp (tho 32 is top of PCO "normal" range)
mcm2 cdc19-P1, cdc18-K46inviable high temp (done at 32, top of PCO "normal" range)


PMID:9658174
annotated physical interactionsno change throughout cell cycle
negative resultno physical interaction between Mcms and Cdc18


PMID:9705504
mcm3-mcm5 phys int "tight"what to do about interaction strength/affinity?


PMID:9914167
mcm2 cdc19-P1FYPO:1386 change 'other' evidence to staining when in ECO
mcm*delta::his3+ (* = 2, 4, 5, or 6)deletion phenotype is that of haploid spore derived from mcm*delta::his3+/mcm*+ diploid; detected protein presumed to be residual from diploid
mcm2delta::his3+/mcm2(cdc19-P1) diploidcouldn't construct; hypothesize that ts mcm2 haploinsufficient
mcm4delta::his3+/mcm4(cdc21-M68) constructed & sporulated at 36dgmcm4delta::his3+ spores: DNA synthesis abolished (didn't enter S phase); 75% abnormal nuclear or cell division


PMID:19168988
note: SPAPB2B4.04c has pmc1 as synonym (used in the paper); documented allele synonyms
multi-way genetic interactionoverexp pmr1+ sup (SPAPB2B4.04cdelta,cta5delta fypo:98)
gene expressionpmr1+ > SPAPB2B4.04c+ > cta5+ during veg growth GO:0072690, but nothing else shown (e.g. change upon stimulus/during GO BP); one measurement


PMID:10409743
hsk1 gene expboth mRNA and protein "relatively constant throughout cell cycle"
dfp1 C-term del, exact residues not specifiedinviable; no further data
Dfp1 "hyperphosphorylated" in presence of HU


PMID:10411894
Dfp1 "hyperphosphorylated" in presence of HUrequires Cds1 kinase


PMID:10490657
lots of Mcm2 & Mcm6 in unspecified "soluble" fractionthroughout cell cycle


PMID:10588642
GFP-beta-galactosidase + SV40 NLSnucleus
GFP-beta-galactosidase + mcm2-M9 residues 5-10 (mutated NLS1)cytoplasm
GFP-beta-galactosidase + Mcm2 residues 114-118 (NLS2)cytoplasm


PMID:11606526
Cdc23 protein loc"insoluble"


PMID:2544292
identified two 5' utrs, starting at -249 and -214; in Artemis they would be:
FT   5'UTR           complement(3175099..3175313)
FT                   /systematic_id="SPBC776.02c"
FT                   /db_xref="PMID:2544292"
FT   5'UTR           complement(3175099..3175348)
FT                   /systematic_id="SPBC776.02c"
FT                   /db_xref="PMID:2544292"
PMID:2544292 cont'dheadmelting genetic interactions (1st 4 phenotypes entered)
cdc25-22 wee1-6 dis2+ overexpts (unlike cdc25 wee1 double mutant)
cdc25-22 wee1-50 dis2+ overexpts
cdc25-22 cdc2-1w dis2+ overexpts
cdc25-22 cdc2-3w dis2+ overexpts
cdc25-r22 sup3-5,r36 ade6-704ade prototroph
cdc25-r22 sup3-5,r36 ade6-704 dis2+ overexpade prototroph
wee 1-6 cdc25-r22 sup3-5,r36 ade6-704ade auxotroph
wee 1-6 cdc25-r22 sup3-5,r36 ade6-704 dis2+ overexpade auxotroph
cdc2-3w cdc25-r22 sup3-5,r36 ade6-704ade auxotroph
cdc2-3w cdc25-r22 sup3-5,r36 ade6-704 dis2+ overexpade auxotroph
relevant background from a cited paper:
sup3-5,r36 ade6-704ade auxotroph
cdc25-rr43 sup3-5,r36 ade6-704ade prototroph
sup3-5,r36 is an opal nonsense suppressor tRNA; ade6-704 has an opal nonsense mutation to suppress


PMID:11973289
mcm4,6,7 alleles all also tested in presence of corresponding ts (mcm4-M68, mcm6-268, mcm7-98) at high tempmight not be worth annotating, since it doesn't add new info (and there are lots, so it would be a pain)


PMID:1849107
cyr1/git2 intragenic complementation - groupsgit2-7, git2-110, git2-266, git2-18, git2-68, git2-216, git2-226, git2-210
compl. groupgit2-61, git2-29, git2-83


PMID:1883874
phenotypes entered for epistasis
epistasisste6delta gpa1delta (aka sar1delta) (technically both disruptions) same phenotypes as gpa1delta alone
epistasisras1delta gpa11delta (technically both disruptions) same phenotypes as ras1delta alone


PMID:10655214
orc2deltafypo:614 (facs; high temp) in germinating orc2delta spores from orp2-2/orc2delta::ura4 diploid (note difference from orc2delta phenotype if diploid was orc2+/orc2delta::ura4
orc2deltacut (micr; high temp) if from from orp2-2/orc2delta::ura4 diploid as above
orc2deltaabnormal repl checkpoint fypo:173 (micr; high temp, +HU) if from from orp2-2/orc2delta::ura4 diploid as above


PMID:2170029
dis2cs R245Q overexp + dis2+ on chrslow growth std temp (restrictive) still need to add this one, because wt endogenous not yet available
dis2 gene expactivity constant throughout cell cycle (have annotated constant protein level)
ppa2 gene expactivity constant throughout cell cycle (didn't look at protein)


PMID:3870979
need way to represent h* designation in genotype
ran1 pat1-114 in h90 fypo:761 glucose minimal medium std temp (followed by apparently normal sporulation)
pat1-114 in h-normal absence of conjugation minimal? (it's PMA) std temp
pat1-114 in h-normal absence of sporulation PMA std temp
pat1-114 + mei2::LEU2 in h90 normal conj micr


PMID:7588609
epistasisrep2+ overexp no effect on res2delta


PMID:23260662
need seq feature with ID
mat1-Msmt-0 "deletion of 263 bp distal to mat1"; also see PMID:8431959
mat1-SS2 deletes 111-bp centromere-distal segment 1152-1262 as they've defined cassette (PMID:21423720)fypo:3091 a_u lsd1 chip
double mutants
mat1-Msmt-0 lsd1-E918abol GO:0011000 fypo:3084


PMID:16772338
pending outcome of chado 433could add myp2 protein binding go:5515 ipi with chs2 (if it won't be inferred somewhere)


PMID:18256290
pending outcome of chado 433could add rho1 protein binding go:5515 ipi with pxl1 (if it won't be inferred somewhere)
double mutantsin some cases "high temp" is a fudge for higher vs. lower within PECO "standard" range


PMID:7969124
srp54 allele combinationsdiploids, wt on one chromosome, mutant on high-copy plasmid
srp54+/T248S decr veg gr fypo:1355 min med; expr low at low temp (growth normal at std or high temp)
srp54+/T248K decr veg gr fypo:1355 min med; expr high at low temp, expr low at high temp (growth normal at std temp)
srp54+/T248R decr veg gr fypo:1355 min med; expr high at low temp, expr low at high temp (growth normal at std temp)
srp54+/T248Ndecr veg gr fypo:1355 min med; expr low at high or low temp (growth normal at std temp)
srp54+/R194Q decr veg gr fypo:1355 min med; expr high at high or low temp (growth normal at std temp)
srp54+/R194L decr veg gr fypo:1355 min med; expr high at high or low temp (growth normal at std temp)
srp54+/R194H decr veg gr fypo:1355 min med; expr low at low temp, expr high at high temp (growth normal at std temp)
srp54+/D190Ndecr veg gr fypo:1355 min med; expr low at low temp (growth normal at std or high temp)
srp54+/D138Ndecr veg gr fypo:1355 min med; expr low at low temp, expr high at high temp (growth normal at std temp)
srp54+/K114R decr veg gr fypo:1355 min med; expr high at high or low temp (growth normal at std temp)
srp54+/K114G decr veg gr fypo:1355 min med; expr low at low temp, expr high at high temp (growth normal at std temp)
srp54+/Q110G decr veg gr fypo:1355 min med; expr high at low temp, expr low at high temp (growth normal at std temp)
srp54+/Q110E decr veg gr fypo:1355 min med; expr high at high temp (growth normal at std or low temp)
srp54+/Q110R decr veg gr fypo:1355 min med; expr low at high or low temp (growth normal at std temp)
srp54+/Q110Kdecr veg gr fypo:1355 min med; expr low at high or low temp (growth normal at std temp)


PMID:10079327
srp54 allele combinationsdiploids, wt on one chromosome, mutant on high-copy plasmid
srp54+/R194A,H195Adecr veg gr fypo:1355 min med; expr high at high or low temp (growth normal at std temp)
srp54+/E277A,H278Adecr veg gr fypo:1355 min med; expr high at high temp (growth normal at std or low temp)
srp54+/K314A,K315Adecr veg gr fypo:1355 min med; expr high at high temp (growth normal at std or low temp)
srp54+/K428A,K429Adecr veg gr fypo:1355 min med; expr high at high temp (growth normal at std or low temp)


Stored additional specificity for evidence (in case we ever want to go back and "upgrade")

PMID:7865880mis5-268(unknown), PCO:0000004FYPO:217PFGE
PMID:8895665orc1 cdc30-4(unknown)FYPO:0000217PFGE
PMID:8838655mcm2FYPO:0000217PFGE
PMID:8838655mcm2FYPO:0001133PFGE
PMID:8838655mcm5FYPO:0000217PFGE
PMID:8838655mcm5FYPO:0001133PFGE
PMID:9914167mcm2 cdc19-P1FYPO:848PFGE
PMID:11606526mcm7-98FYPO:0000848PFGE
PMID:7628438ssp1 allelesFYPO: normal chr morphPFGE

Community Curation sessions I've reviewed

PMID:23677513 fdeaa8d994824f8c
can also add myh1 chromatin binding in_presence_of phleomycin ChEBI:75044


PMID:23427262
ndc80-NH12 mad2deltafypo:168?
update fypo:1645 extension assayed_using(ndc80, alp7)
Nuf2-Alp7C fusion + ndc80-NH12(nearly) normal veg cell pop growth; normal chromosome seg (fig 6)
ndc80-NH12 + dis1deltainviable veg cell pop
ndc80-NH12/ndc80-21 heterozygous diploidsensitive to high temp, but not as severe as either homozygous diploid (fig 7)


PMID:23576550
update fypo:1571 extension assayed_using(alp41, alp1)
tbc1-11 alp41-8sens to high temp
tbc1-11 alp41-14normal veg pop growth high temp


PMID:24081329
nab2::kanMX6 pab1::ura4+decr veg cell pop growth std temp & high temp
nab2::kanMX6 pab1::ura4+incr polyA tail length fypo:2931 gel (more severe than pab1- alone, so expr high)
nab2::kanMX6 pab1::ura4+incr pre-mRNA level fypo:1908 northern (more severe than pab1- alone, so expr high) a_u rpl3002
nab2::kanMX6 pab1::ura4+incr mRNA level fypo:2936 northern (more severe than pab1- alone, so expr high) a_u rpl3002
nab2::kanMX6 rpl3002deltainormal mRNA level fypo:2938 a_u rpl3002
nab2::kanMX6 rpl3001::kanMX6same pre-mRNA level as rpl3001 single mutant a_u rpl3002 (epistasis)
nab2::kanMX6 prp1-4less excess pre-mRNA than prp1-4 alone (incr pre-mRNA level fypo:1908 northern expr low) high temp a_u rpl3002
nab2::kanMX6 prp2-1less excess pre-mRNA than prp2-1 alone (incr pre-mRNA level fypo:1908 northern expr low) high temp a_u rpl3002
nab2::kanMX6 rrp6::ura4+same pre-mRNA & mRNA levels as rrp6- alone (epistasis) a_u rpl3002


PMID:23874188credit Jun-Song Chen where noted (MAH did growth phenotypes; J-S C did microscopy-based
cdc11-S5A cdc16-116sens to heat expr high YES
cdc11-S8A cdc16-116sens to heat expr low YES
cdc16-116 csc1::ura4sens to heat expr med YES
cdc11-S5A cdc16-116 csc1::ura4sens to heat expr high YES
cdc11-S8A cdc16-116 csc1::ura4sens to heat expr low YES
cdc11-S5A cdc16-116 csc1::ura4multiseptate expr med (40%) micr; J-S C
cdc11-S5A cdc16-116 csc1::ura4cut expr low (15%) micr; J-S C


PMID:24146635
mas5delta cdc42-G12V overexpfypo:3150 expr low
mas5delta cdc42-G12V overexpnormal monopolar cell growth fypo:3156 penetr med
mas5delta cdc42-G12V overexpintermittent fypo:3155 penetr low
mas5delta hsf1+ knockdownincr prot lev fypo:1327 a_u hsp104 std temp expr low
mas5delta hsf1+ knockdownfypo:3150 expr low
mas5delta hsf1+ knockdownnormal monopolar cell growth fypo:3156 penetr med
mas5delta hsf1+ knockdownintermittent fypo:3155 penetr low


PMID:24316795
pom1delta305N bud6deltafypo:2253, fypo:648, fypo:2258 a_u cdr2 (same as pom1 single mutant) micr emm std temp
pom1+/pom1delta diploidfypo:648 micr emm std temp
pom1+/pom1delta diploidfypo:2558 a_u cdr2 micr emm std temp
pom1+/pom1delta diploidfypo:2253 micr emm std temp
pom1+/pom1delta diploiddecr prot lev fypo:1324 a_u pom1 expr med micr emm std temp
cdr2-GFP/cdr2deltafypo:1124 micr emm std temp
pom1delta pnmt81-cdr2fypo:648 micr expr high
pom1delta pnmt81-cdr2-mEGFPfypo:648 micr expr high
pom1delta cdr2-S755Afypo:648 expr med (slightly shorter than cdr2 single mutant) micr
cdr2-S755A pom1deltafypo:339 micr
cdr2-S755A-758A pom1deltafypo:339 micr
cdr2-S755A-758A pom1deltafypo:2556 a_u cdr2
cdr2-S755A-758A + (pom1delta or pom1delta305N)same cell length as cdr2-S755A-758A alone


PMID:23394829
mad2+ overexp nak1-167premature septation fypo:3250 micr expr high
mad2+ overexp sid1-250normal septation (i.e. no premature septation)
mad2+ overexp nak1-167 sid1-250normal septation (i.e. no premature septation)
nak1-5A cdc3-124 cdc16-116abol prot phos fypo:2033 enz assay a_u nak1
cdc3-124 cdc16-116normal prot phos fypo:776 enz assay a_u nak1
nak1-5A cdc3-124 cdc16-116viable elong veg fypo:1492 micr high temp
nak1-5E fypo:705 update to a_u nak1,mob2


PMID:24210919
rrp6delta rmn1deltafypo:3231 chip
rrp6delta iss10deltafypo:3234 chip
rrp6delta nrl1deltafypo:3103 rnaseq
rrp6delta nrl1deltafypo:3231 chip
rrp6delta nrl1deltafypo:3236 rnaseq
rrp6delta cwf10-1fypo:3103 rnaseq
rrp6delta cwf10-1fypo:3231 chip
rrp6delta cwf10-1fypo:3236 rnaseq


PMID:24344203
tsc1delta sin1deltafypo:250
tsc2delta sin1deltafypo:250
tsc1delta gad8deltafypo:250
tsc2delta dag8deltafypo:250
tsc1delta tor1SE endogfypo:111 cond peco:246
tsc1delta tor2SE endogfypo:2672 cond peco:246
tsc1delta isp7+ overexpfypo:2672 cond peco:246
tsc2delta isp7+ overexpfypo:2672 cond peco:246
tsc1delta isp7deltafypo:3252
tsc2delta isp7deltafypo:3252
tsc2delta isp7deltafypo:3254
tsc2delta isp7deltafypo:35
tor2-51 isp7deltafypo:1592 expr high
tor2-51 isp7deltafypo:3258 expr med std temp
tor2-51 isp7deltafypo:new6 high/restr temp
tsc2delta isp7deltafypo:3255
tor1delta isp7deltafypo:3257 expr med
tsc2delta isp7+ overexpfypo:3255
tor2SE isp7+ overexpfypo:1838 cond +rapa a_u rps602
gtr1delta tsc2deltafypo:3252
gtr2delta tsc2deltafypo:3252
gtr1delta isp7deltafypo:243
gtr2delta isp7deltafypo:243
tsc2delta isp7+ overexpfypo:1038 a_u rps602 (same as isp7+ oe alone)
tsc2delta isp7-H276Afypo:1038 a_u rps602 (same as isp7+ oe alone)
tsc2delta isp7-H276Afypo:111


PMID:23297348
clp1delta csc1deltafypo:1253 micr expr high
cdc11-S8A sid1-239sens heat; decr veg pop gr YE std temp expr low (same as sid1-239 alone)
cdc11-S8D sid1-239decr veg pop gr YE std temp expr med
cdc11-S8A spg1-106decr veg pop gr YE std temp expr med (same as spg1-106 alone)
cdc11-S8D spg1-106decr veg pop gr YE std temp expr high
cdc11-S8A cdc7-24sens heat expr high (same as cdc7-24 alone)
cdc11-S8D cdc7-24sens heat expr high (same as cdc7-24 alone)


PMID:23348717
AspRec8c–FGFP overexp ubr11deltadecr veg pop growth expr high
AspRec8c–FGFP overexp ubr11deltaincr minichr loss


PMID:23671279
gcn2delta cpc2deltasens amitrole fypo:1097 min med (same as either single mutant)
hri2delta cpc2deltasens amitrole fypo:1097 min med (same as cpc2delta alone)
gcn2delta cpc2deltadecr prot phos fypo:1838 min med amitrole a_u tif211 expr med (same as cpc2delta alone)
hri2delta gcn2deltaabol prot phos fypo:2033 min med amitrole a_u tif211 (same as gcn2delta alone)
hri2delta cpc2deltadecr prot phos fypo:1838 min med amitrole a_u tif211 expr high (worse than cpc2delta alone)