wiki:MidoriCuratedPapers

Version 68 (modified by gomidori, 9 years ago) (diff)

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transferred from CuratorTrainingPapers

Quick fixes

Short wait for whatever's needed, so add to existing sessions

Multiple mutations, thorny extensions, etc.

Longer-term, so may require new curation sessions

21813639open new session to add these when supported
mcb1GO:0005737extension: throughout cell cycle (see nuclear pore/PMID:20970342 above)
mcb1GO:0005654extension: throughout cell cycle
mcb1GO:0000790extension: throughout cell cycle
mcb1-chk1 genetic interactionalleles OP-mcb1+chk1-deletion, mcb1-D2+chk1-deletion; mcb1-D22+chk1-deletionOP-mcb1+chk1-del phenotype small cells (FYPO:23), abnormal nuc morph (FYPO:62)
mcb1-rad3 genetic interactionalleles OP-mcb1+rad3-deletion, mcb1-D2+rad3-deletion; mcb1-D22+rad3-deletionOP-mcb1+rad3-del phenotype small cells (FYPO:23), abnormal nuc morph (FYPO:62)


20929775open new session to add these when supported
hht1,hht2,hht3all 3 T3A (triple mutant)phenotype decreased prot loc to cen (extension: Ark1); lagging chromosomes (qualifier=low expressivity)
hta1,hta2both S121A (triple mutant)phenotype decreased prot loc to cen (extension: Ark1); lagging chromosomes (qualifier=low expressivity)
hht1,hht2,hht3,sgo2delall 3 hht T3A + sgo2del (quadruple mutant)phenotypes abolished loc to cen (ext Ark1); lagging chromosomes (qualifier=high expressivity)
hht1,hht2,hht3, hta1,hta2all 3 H3 T3A, both H2 S121A (quintuple mutant)phenotypes abolished loc to cen (ext Ark1); lagging chromosomes (qualifier=high expressivity)
hht1,hht2,hht3all 3 T3A (triple mutant)genetic interaction synthetic lethal sgo2deletion
hht1,hht2,hht3, hta1,hta2all 3 H3 T3A, both H2 S121A (quintuple mutant)genetic interaction synthetic lethal
hht1,hht2,hht3, hta1,hta2all 3 H3 T3A, both H2 S121A (quintuple mutant) genetic interaction synthetic lethal
genetic interactionhrk1, swi6"asynthetic"; check for old same-pathway GO IGI
hht1,hht2,hht3all 3 T3A (triple mutant)genetic interaction "asynthetic" with hrk1deletion
Hrk1-Bir1physical interactionBIR domain of Bir1 (Pfam:PF00653)
hrk1 bub1 genetic interactionalleles hrk1 deletion; bub1-KD (kinase dead, K762R,D900N)phenotype slow growth
hrk1 sgo2 genetic interactionalleles both deletionphenotype slow growth
pds5 bub1 genetic interactionalleles pds5 deletion; bub1-KD (kinase dead, K762R,D900N)phenotype slow growth
pds5 sgo2 genetic interactionalleles both deletionphenotype slow growth
swi6 sgo2 genetic interactionalleles both deletionphenotype slow growth


21620704open new session to add these when supported
dip1 wsp1 genetic intalleles both deletionphenotypes: abnormal protein localization to actin cortical patch (crn1), abolished actin cortical patch internalization
how on earth to annotate anything based on figure 6?


PMID:21971174new session for these
cdt1 allele=noname(overexpression) mcm6-S1(E824G, D825N) normal ds break repair (FYPO:new)
cdc18 allele=noname(overexpression) mcm6-S1(E824G, D825N) normal ds break repair (FYPO:new)
cig2delta(deletion) sup) mcm6-S1(E824G, D825N) normal ds break repair (FYPO:new)


PMID:8895665new session
orc1/cdc30-4(unknown) + cdc18-K46(unknown) double mutantFYPO:173 ev:microscopy


PMID:6887244 long-term note (new session)
additional note3 of 13 cold-sensitive mutants curated; if someone can figure out what genes the other 10 correspond to, we can annotate a few more phenotypes


PMID:8298187new session? or not in tool?
noteadd mcm2 allele synonym nda1-376 = nda1-KM376; also nda1delta = mcm2delta, nda4delta = mcm5delta


PMID:1934126new session
cdc25-22(unknown) wee1-50(unknown) double mutantFYPO:681 same as cdc25-22(unknown) single mutant
cdc25-22(unknown) cdc2-1w(unknown) double mutantFYPO:681 same as cdc25-22(unknown) single mutant
cdc25-22(unknown) adh.nim1(overexpresssion) double mutantFYPO:681 same as cdc25-22(unknown) single mutant
cdc25-22(unknown) wee1-50(unknown) win1noname(unknown) triple mutantFYPO:681 same as cdc25-22(unknown) single mutant
cut1noname(unknown) cut2noname(unknown) double mutantFYPO:681 ev: microscopy


PMID:7865880 new session
mcm6 allele=mis5-268(unknown) + mcm4 cdc21noname(unknown)fypo:316 ev: cell growth
other stuffmis2, mis7, mis8, mis9 all apparently uncloned, so don't know which gene they correspond to
mis2 ?geneFYPO:786 ev: plasmid maint assayallele=mis2-87(unknown),condition=PCO:0000005,condition=PCO:0000004 | allele=mis2-197(unknown),condition=PCO:0000004
mis7 ?geneFYPO:786 ev: plasmid maint assayallele=mis7-322(unknown),condition=PCO:0000005
mis8 ?geneFYPO:786 ev: plasmid maint assayallele=mis8-341(unknown),condition=PCO:0000005,condition=PCO:0000004
mis9 ?geneFYPO:786 ev: plasmid maint assayallele=mis9-356(unknown),condition=PCO:0000005


PMID:8631307open new session to add these when supported
mcm4GO:0005634 extension: throughout cell cycle
cdc21-M68(unknown) cdc2-3w(unknown) double mutantFYPO:609 ev: microscopy
cdc21-M68(unknown) mcm4 C-terminus overexpression [note: overexp piece from BamHI site -- need position -- to endfypo:49
cdc21-M68(unknown) rad1noname(unknown) double mutantFYPO:23 FYPO:337 both ev: microscopy


PMID:19197239new session
top1delta tdp1deltaFYPO: sensitive to camptothecin ev. cell growth


PMID:8838655new session
mcm2GO:0005634extension: throughout cell cycle
mcm5GO:0005634extension: throughout cell cycle


PMID:12136010new session
tsc1 leu1fypo:46; ev cell growth assay; cond. limiting leu
tsc1 ade2fypo:46; ev cell growth assay; cond. limiting ade
tsc2 leu1fypo:46; ev cell growth assay; cond. limiting leu
tsc2 ade2fypo:46; ev cell growth assay; cond. limiting ade
tsc1 tsc2fypo:46; ev cell growth assay; cond. limiting ade


PMID:9383050lots of mcm2 alleles done with one on chromosome, another on plasmid (cells otherwise haploid)
mcm2 allele on chromosome/allele on plasmidFYPO ID, high tempFYPO ID, normal temp (32, semi-restrictive)
cdc19-P1/mcm2+fypo:47 normal cell growthfypo:47
cdc19-P1/mcm2-M1fypo:49 inviablefypo:49, fypo:299 microcolony formation, fypo:5 abn. cell morph.
cdc19-P1/mcm2-M2fypo:49fypo:49, fypo:299, fypo:5
cdc19-P1/mcm2-M3fypo:49fypo:49, fypo:299, fypo:5
cdc19-P1/mcm2-M5fypo:49fypo:49, fypo:299, fypo:5
cdc19-P1/mcm2-M6fypo:49fypo:47
cdc19-P1/mcm2-M7fypo:47fypo:46 slow cell growth
cdc19-P1/mcm2-M8fypo:49fypo:46
cdc19-P1/mcm2-M9fypo:49fypo:49, fypo:299, fypo:5
cdc19-P1/mcm2-M10fypo:49fypo:49, fypo:299, fypo:5
cdc19-P1/cdc19-P1fypo:49n/a
cdc19-P1/nda1-KM376fypo:47n/a
cdc19-P1/mcm2-D1fypo:47fypo:47
cdc19-P1/mcm2-D2fypo:47fypo:47
cdc19-P1/mcm2-D3fypo:47fypo:47
cdc19-P1/mcm2-D4fypo:49fypo:49, fypo:299, fypo:5
cdc19-P1/mcm2-D6fypo:49fypo:46
cdc19-P1/mcm2-D7fypo:49fypo:49, fypo:299, fypo:5
cdc19-P1/mcm2-D8fypo:49fypo:46
cdc19-P1/mcm2-D9fypo:49fypo:47
cdc19-P1/mcm2-D10fypo:49fypo:46
wt or deletion setviable at 32dg? (with mcm2 allele of interest overexpressed)
chromosome/plasmidfypo ID, normal temp
mcm2+/mcm2-M1fypo:47
mcm2+/mcm2-M2fypo:47
mcm2+/mcm2-M3fypo:47
mcm2+/mcm2-M5fypo:47
mcm2+/mcm2-M6fypo:47
mcm2+/mcm2-M7fypo:47
mcm2+/mcm2-M8fypo:47
mcm2+/mcm2-M9fypo:47
mcm2+/mcm2-M10fypo:47
mcm2+/mcm2-D1fypo:47
mcm2+/mcm2-D2fypo:47
mcm2+/mcm2-D3fypo:47
mcm2+/mcm2-D4fypo:47
mcm2+/mcm2-D6fypo:47
mcm2+/mcm2-D7fypo:47
mcm2+/mcm2-D8fypo:47
mcm2+/mcm2-D9fypo:47
mcm2+/mcm2-D10fypo:47
mcm2delta/mcm2+fypo:47
mcm2delta/mcm2-M6fypo:49
mcm2delta/mcm2-M7fypo:47
mcm2delta/mcm2-M8fypo:49
mcm2delta/cdc19-P1fypo:47
mcm2delta/mcm2-D1fypo:47
mcm2delta/mcm2-D2fypo:47
mcm2delta/mcm2-D3fypo:47
gene expression complexities
mcm2-M7, D1, D2, D3 and wt alleleslevel of expression from plasmid with nmt promoter much higher with cdc19-P1 on chromosome than with mcm2+ on chr
Mcm2 protein nearly same throughout cell cycle; modest decrease in G2could use artemis gene exp syntax, but don't have a "throughout cell cycle" GO term or extension
ordinary double mutant phenotypes
mcm2 cdc19-P1, mcm4+ overexpslow growth; fypo:150; fypo:614 facsnormal temp
mcm2 cdc19-P1, mcm5+ overexpslow growth; fypo:150; fypo:614 facsnormal temp
mcm2 cdc19-P1, mcm6+ overexpslow growth; fypo:150; fypo:614 facsnormal temp
mcm2 cdc19-P1, cdc6 poldelta-ts1slow cell growth, elong cells, curved cells; normal temp 25 or 29; at 32 dg branched cells, t-shaped cells, inviable high temp (tho 32 is top of PCO "normal" range)
mcm2 cdc19-P1, cdc1-7slow cell growth, elong cells, curved cells; normal temp 25 or 29; at 32 dg branched cells, t-shaped cells, inviable high temp (tho 32 is top of PCO "normal" range)
mcm2 cdc19-P1, cdc18-K46inviable high temp (done at 32, top of PCO "normal" range)


PMID:9658174
gene expression - Mcm2, Mcm4, Mcm5, Mcm6protein levels constant throughout cell cycle (ev: western)
annotated physical interactionsno change throughout cell cycle
negative resultno physical interaction between Mcms and Cdc18


PMID:9705504
gene expression - Mcm3protein level constant throughout cell cycle (ev: western)
GO mcm3 nucleus 5634 IDAextension: throughout cell cycle
mcm3-mcm5 phys int "tight"what to do about interaction strength/affinity?


PMID:9914167
mcm2 cdc19-P1FYPO:1386 change 'other' evidence to staining when in ECO
mcm*delta::his3+ (* = 2, 4, 5, or 6)deletion phenotype is that of haploid spore derived from mcm*delta::his3+/mcm*+ diploid; detected protein presumed to be residual from diploid
mcm2delta::his3+/mcm2(cdc19-P1) diploidcouldn't construct; hypothesize that ts mcm2 haploinsufficient
mcm4delta::his3+/mcm4(cdc21-M68) constructed & sporulated at 36dgmcm4delta::his3+ spores: DNA synthesis abolished (didn't enter S phase); 75% abnormal nuclear or cell division


PMID:10388806
many, many double and triple mutant phenotypes
rum1+ overexp, cdc18-K46fypo:1427 facs
rum1+ overexp, cdc23-M36fypo:1427 facs
rum1+ overexp, cdc6(pold-1)fypo:1427 facs
rum1+ overexp, orc1(orp1-4)fypo:1427 facs
rum1+ overexp, cdc17-K42fypo:1426 facs
rum1+ overexp, cdc24-g1fypo:1426 facs
rum1+ overexp, cdc20-M10fypo:1426 facs
rum1+ overexp, rad4-116fypo:1426 facs
rum1+ overexp, cdc1-7fypo:1428 facs
rum1+ overexp, cdc10-V50fypo:1428 facs
rum1+ overexp, pol1-1fypo:1428 facs
rum1+ overexp, mcm2(cdc19-P1)fypo:1428 facs
rum1+ overexp, mcm4(cdc21-M68)fypo:1428 facs
rum1+ overexp, cig1delta::ura4+fypo:1425 facs, enlarged nuc fypo:344 ev: micr, elong fypo:839 ev: micr
rum1+ overexp, cig2delta::ura4+fypo:1425 facs, enlarged nuc fypo:344 ev: micr, elong fypo:839 ev: micr
rum1+ overexp, cig1delta::ura4+, cig2delta::ura4+fypo:1428 facs
cdc13delta::ura4+, cdc10-V50fypo:1428 facs; elong fypo:839 ev: micr, cond high temp
cdc13delta::ura4+, mcm2(cdc19-P1)fypo:1428 facs; elong fypo:839 ev: micr, cond high temp
cdc13delta::ura4+, mcm4(cdc21-M68)fypo:1428 facs; elong fypo:839 ev: micr, cond high temp
cdc13delta::ura4+, cdc6(pold-1)fypo:1428 facs; elong fypo:839 ev: micr, cond high temp
cdc13delta::ura4+, cdc18-K46fypo:1428 facs; elong fypo:839 ev: micr, cond high temp
cdc13delta::ura4+, cdc18delta::p[nmt*.cdc18+-LEU2]fypo:1428 ev facs, fypo:1430 ev:facs
cdc13delta::ura4+, mcm2delta::his3+fypo:1428 ev facs, abn chr morph fypo:848 ev: 'other' (for PFGE)
cdc13delta::ura4+, mcm4delta::his3+fypo:1428 ev facs, abn chr morph fypo:848 ev: 'other' (for PFGE)


PMID:19286980
psd1delta,psd2deltaviable rich med, std temp; viable minimal med, std temp
psd1delta,psd3deltaviable rich med, std temp; viable minimal med, std temp
psd2delta,psd3deltaviable rich med, std temp; viable minimal med, std temp
psd1delta,psd2deltanormal cell morph rich med, std temp, limited eth pco:169|
psd1delta,psd3deltanormal cell morph rich med, std temp, limited eth pco:169
psd2delta,psd3deltanormal cell morph rich med, std temp, limited eth pco:169
psd1delta,psd2delta,psd3deltaviable rich med, std temp; inviable minimal med, std temp
psd1delta,psd2delta,psd3deltafypo:38, minimal med, std temp; rich med, std temp
psd1delta,psd2delta,psd3deltadecr PE fypo:1285 rich med, minimal, minimal + ethanolamine; all std temp
psd1delta,psd2delta,psd3deltaincr PS fypo:1505 rich med, minimal, minimal + ethanolamine; all std temp
psd1delta,psd2delta,psd3deltanormal PC fypo:1506 rich med, minimal, minimal + ethanolamine; all std temp
psd1delta,psd2delta,psd3deltanormal PI fypo:1507 rich med, minimal, minimal + ethanolamine; all std temp
psd1delta,psd2delta,psd3deltafypo:1496 minimal pco:169 std temp
psd1delta,psd2delta,psd3deltafypo:339 minimal pco:169 std temp
psd1delta,psd2delta,psd3deltafypo:20 minimal pco:169 std temp
psd1delta,psd2delta,psd3deltafypo:21 minimal pco:169 std temp
psd1delta,psd2delta,psd3deltafypo:1390 minimal pco:169 std temp
psd1delta,psd2delta,psd3deltafypo:672 rich pco:154 std temp
psd1delta,psd2delta,psd3deltafypo:673 rich pco:154 std temp
psd1delta,psd2delta,psd3deltafypo:5 ext has_penetrance(FYPO_EXT:0000003) minimal pco:154 std temp
psd1delta,psd2delta,psd3deltafypo:5 ext has_penetrance(FYPO_EXT:0000001) minimal pco:169 std temp
psd1delta,psd2delta,psd3deltafypo:161 rich pco:169 std temp
psd1delta,psd2delta,psd3deltafypo:182 rich pco:169 std temp
all evidence cell growth or microscopy as appropriate


PMID:19168988
note: SPAPB2B4.04c has pmc1 as synonym (used in the paper), so document allele synonyms
SPAPB2B4.04cdelta,cta5deltafypo:1419 rich med std temp
SPAPB2B4.04cdelta,SPCC1672.11cdeltafypo:1487 fypo:636 rich med std temp
pmr1delta,cta5deltafypo:1487 fypo:636 rich med std temp
pmr1delta,SPCC1672.11cdeltafypo:1487 fypo:636 rich med std temp
cta5delta,SPCC1672.11cdeltafypo:1487 fypo:636 rich med std temp
SPAPB2B4.04cdelta,cta5deltafypo:98 ext has_expressivity(FYPO_EXT:0000001) rich std temp
SPAPB2B4.04cdelta,cta5deltafypo:92 ext has_expressivity(FYPO_EXT:0000001) rich std temp
SPAPB2B4.04cdelta,SPCC1672.11cdeltafypo:98 ext has_expressivity(FYPO_EXT:0000001) rich std temp
SPAPB2B4.04cdelta,cta5deltafypo:286 minimal std temp
SPAPB2B4.04cdelta,SPCC1672.11cdeltafypo:286 minimal std temp
SPAPB2B4.04cdelta,cta5delta, overexp pmr1+fypo:1020 minimal std temp
SPAPB2B4.04cdelta,cta5deltafypo:1020 pco:114 minimal std temp
multi-way genetic interactionoverexp pmr1+ sup (SPAPB2B4.04cdelta,cta5delta fypo:98)
gene expressionpmr1+ > SPAPB2B4.04c+ > cta5+ during veg growth GO:0072690, but nothing else shown (e.g. change upon stimulus/during GO BP); one measurement


PMID:10409743
hsk1 gene expboth mRNA and protein "relatively constant throughout cell cycle"
dfp1 C-term del, exact residues not specifiedinviable; no further data
dfp1 him1-3A, chk1delta::ura4+cut annotation_extension=has_penetrance(FYPO_EXT:0000002), micr, HU added
cds1delta::ura4+,chk1delta::ura4+cut annotation_extension=has_penetrance(FYPO_EXT:0000001), micr, HU added
Dfp1 "hyperphosphorylated" in presence of HU


PMID:10411894
Dfp1 "hyperphosphorylated" in presence of HUrequires Cds1 kinase


PMID:10490657
orc1 GO:790throughout cell cycle
mcm6 go:5634throughout cell cycle
lots of Mcm2 & Mcm6 in unspecified "soluble" fractionthroughout cell cycle
cdc10-129, overexp cdc18+normal prot loc to chromatin fypo:1509; annotation_extension=assayed_using(PomBase:SPBC211.04c)


PMID:10588642
mcm6 nucleus go:5634throughout cell cycle
mcm2/cdc19-P1, crm1-11Rfypo:838 prot loc; high temp; annotation_extension=assayed_using(PomBase:SPBC211.04c)
mcm4/cdc21-M68, crm1-11Rfypo:838 prot loc; high temp; annotation_extension=assayed_using(PomBase:SPBC211.04c)
mcm2/cdc19-P1, mcm3-HAfypo:783 prot loc; high temp; annotation_extension=assayed_using(PomBase:SPBC4.04c)|assayed_using(PomBase:SPCC16A11.17)|assayed_using(PomBase:SPBC211.04c)
mcm2/cdc19-P1, mcm3-HAfypo:1424 prot loc; high temp; annotation_extension=assayed_using(PomBase:SPBC4.04c)|assayed_using(PomBase:SPCC16A11.17)
mcm4/cdc21-M68, mcm3-HAfypo:783, fypo:1424 all prot loc; high temp; annotation_extension=assayed_using(PomBase:SPBC4.04c)|assayed_using(PomBase:SPCC16A11.17)|assayed_using(PomBase:SPBC211.04c)
crm1-809, mcm3-HAfypo:838 prot loc; low temp; annotation_extension=assayed_using(PomBase:SPBC4.04c)|assayed_using(PomBase:SPCC16A11.17)|assayed_using(PomBase:SPBC211.04c)
GFP-beta-galactosidase + SV40 NLSnucleus
GFP-beta-galactosidase + mcm2-M9 residues 5-10 (mutated NLS1)cytoplasm
GFP-beta-galactosidase + Mcm2 residues 114-118 (NLS2)cytoplasm
mcm2/cdc19, mcm2-M9 overexpinviable
mcm2/cdc19, mcm2-M9 overexp, mcm3-HAinviable; fypo:783, fypo:1424 all prot loc; std temp; annotation_extension=assayed_using(PomBase:SPCC1682.02c)|assayed_using(PomBase:SPCC16A11.17)|assayed_using(PomBase:SPBC211.04c)
crm1-809, mcm2+ overexpfypo:783; prot loc; low temp; annotation_extension=assayed_using(PomBase:SPBC4.04c)
crm1-809, mcm2-M2fypo:783; prot loc; low temp; annotation_extension=assayed_using(PomBase:SPBC4.04c)
crm1-809, mcm2-M9fypo:783; prot loc; low temp; annotation_extension=assayed_using(PomBase:SPBC4.04c)
crm1-809, mcm2-M10fypo:783; prot loc; low temp; annotation_extension=assayed_using(PomBase:SPBC4.04c)
crm1-809, mcm2-D6fypo:783; prot loc; low temp; annotation_extension=assayed_using(PomBase:SPBC4.04c)
crm1-809, mcm2-D7fypo:783; prot loc; low temp; annotation_extension=assayed_using(PomBase:SPBC4.04c)


PMID:10747035
mcm4 nucleus go:5634throughout cell cycle
cdc18+ overexp; mcm4 cdc21-M68no rereplication; normal neg reg initiation?


PMID:11854402
pat1-114, mcm4ts-tdabnormal premeiotic DNA replic fypo:625; ev facs; cond high temp
pat1-114, mcm4ts-tdsame protein level phenotypes as mcm4t-td alone


PMID:11606526
Mcm7 expressionprotein present throughout cell cycle
Cdc23 protein loc"insoluble"


PMID:2544292
identified two 5' utrs, starting at -249 and -214; in Artemis they would be:
FT   5'UTR           complement(3175099..3175313)
FT                   /systematic_id="SPBC776.02c"
FT                   /db_xref="PMID:2544292"
FT   5'UTR           complement(3175099..3175348)
FT                   /systematic_id="SPBC776.02c"
FT                   /db_xref="PMID:2544292"
PMID:2544292 cont'dheadmelting genetic interactions
cdc25-22 wee1-6 dis2+ overexpts (unlike cdc25 wee1 double mutant)
cdc25-22 wee1-50 dis2+ overexpts
cdc25-22 cdc2-1w dis2+ overexpts
cdc25-22 cdc2-3w dis2+ overexpts
cdc25-22 wee1-50 dis2+ overexpts
cdc25-r22 sup3-5,r36 ade6-704ade prototroph
cdc25-r22 sup3-5,r36 ade6-704 dis2+ overexpade prototroph
wee 1-6 cdc25-r22 sup3-5,r36 ade6-704ade auxotroph
wee 1-6 cdc25-r22 sup3-5,r36 ade6-704 dis2+ overexpade auxotroph
cdc2-3w cdc25-r22 sup3-5,r36 ade6-704ade auxotroph
cdc2-3w cdc25-r22 sup3-5,r36 ade6-704 dis2+ overexpade auxotroph
relevant background from a cited paper:
sup3-5,r36 ade6-704ade auxotroph
cdc25-rr43 sup3-5,r36 ade6-704ade prototroph
sup3-5,r36 is an opal nonsense suppressor tRNA; ade6-704 has an opal nonsense mutation to suppress


PMID:1944266
dis3-54 dis2-11inviable std temp
dis3-54 dis1-288inviable std temp
dis3-54 dis2deltainviable std temp
dis2-11 dis1-288inviable std temp
dis3-54 sds21deltaviable std temp
cdc25-22 wee1-50 overexp dis2+ts (unlike cdc25 wee1 double mutant)
cdc25-22 wee1-50 overexp dis3+ts
cdc25-22 wee1-50 overexp sds21+ts
cdc25-22 wee1-50 overexp ppa2+not ts
cdc25-22 wee1-50 overexp sds22+not ts
cdc25-22 wee1-50 overexp sds23+not ts
in presence of dis3-54 (cs)
dis3-172A del aa 1-74slow growth low term
dis3-172B del aa 1-225rest all still cs (no growth low temp)
dis3-172C del aa 1-398
dis3-172D del aa 1-445
dis3-172E del aa 1-547
dis3-172F del aa 824-970
dis3-172G del aa 670-970
dis3-172H del aa 442-970
note: deletions on plasmid with pombe adh promoter, which is strong and apparently counts as overexp. to authors


PMID:11973289
mcm4,6,7 alleles all also tested in presence of corresponding ts (mcm4-M68, mcm6-268, mcm7-98) at high temp
mcm6+ with C-terminal 3xHA + mcm6-268 tsinviable if HAtagged not induced, viable if tagged overexp


PMID:12185500
cdc23-M36 mcm2 cdc19-P1inviable st temp 29
cdc23-M36 mcm6 mis5-268inviable st temp 29
cdc23-1E2 mcm2 cdc19-P1inviable st temp 27
cdc23-1E2 mcm6 mis5-268inviable st temp 27
cdc23-M36 mcm4 cdc12-K46inviable st temp 25
cdc23-M36 mcm5 nda4-108inviable st temp 25
cdc23+ overexp, mcm5 nda4-108viable temp 25
gene exp cdc23 mRNA level constant throughout cell cycle


PMID:1849107
cyr1+ overexp, git1-1(unknown)normal glucose repression (i.e. normal neg reg txn by glucose, fypo:1865)
cyr1+ overexp, git3-200(unknown)normal glucose repression
cyr1+ overexp, git5-75(unknown)normal glucose repression
cyr1+ overexp, git7-235(unknown)normal glucose repression
cyr1+ overexp, git8-60(unknown)normal glucose repression
cyr1+ overexp, git10-201(unknown)normal glucose repression
cgs1-1, cyr1-git2-1normal glucose repression
cgs1-1, pka1-git6-261fypo:44 abnormal glucose repression
cyr1/git2 intragenic complementation - groupsgit2-7, git2-110, git2-266, git2-18, git2-68, git2-216, git2-226, git2-210
compl. groupgit2-61, git2-29, git2-83


PMID:1883874
ras1-val17(G17V), gap1+ overexpnormal conjugation micr
sar1 disruption homozygous diploidfypo:279 micr
epistasisste6delta gpa1delta (aka sar1delta) (technically both disruptions) same phenotypes as gpa1delta alone
epistasisras1delta gpa11delta (technically both disruptions) same phenotypes as ras1delta alone


PMID:10655214
orc 2 allele orp2-2, rad1-1cut micr high temp
orc 2 allele orp2-2, rad3deltacut micr high temp
orc2deltafypo:614 (facs; high temp) in germinating orc2delta spores from orp2-2/orc2delta::ura4 diploid (note difference from orc2delta phenotype if diploid was orc2+/orc2delta::ura4
orc2deltacut (micr; high temp) if from from orp2-2/orc2delta::ura4 diploid as above
orc2deltaabnormal repl checkpoint fypo:173 (micr; high temp, +HU) if from from orp2-2/orc2delta::ura4 diploid as above


PMID:10077566
orc4 gene expprotein level constant throughout cell cycle


PMID:2170029
ppa1::ura4+ + ppa2::ura4+inviable
sds21::LEU2 + ppa1::ura4+normal growth normal or high temp
sds21::LEU2 + ppa2::ura4+normal growth normal or high temp
dis2::ura4+ + ppa2::ura4+slow growth (small colonies) normal or high temp
dis2cs R245Q overexp + dis2+ on chrslow growth std temp (restrictive)
dis2cs R245Q overexp + dis2::ura4+ on chrinviable high (permissive) or std temp (restr)
dis2cs R245Q overexp + sds21::LEU2 on chrslow growth std temp (restrictive)
dis2cs R245Q overexp + ppa1::ura4+ on chrslow growth std temp (restrictive)
dis2cs R245Q overexp + ppa2::ura4+ on chrslow growth std temp (restrictive)
dis2 gene expactivity constant throughout cell cycle
ppa2 gene expactivity constant throughout cell cycle


PMID:3185514
ran1/pat1-114 + byr1/ste1-(unknown)same phenotypes as pat1-114 alone - conjugation & sporulation in haploid
ran1/pat1-114 + ste2-(unknown)same phenotypes as pat1-114 alone - conjugation & sporulation in haploid
ran1/pat1-114 + byr1/ste3-(unknown)same phenotypes as pat1-114 alone - conjugation & sporulation in haploid
ran1/pat1-114 + ste4-(unknown)same phenotypes as pat1-114 alone - conjugation & sporulation in haploid
ran1/pat1-114 + ras1/ste5-(unknown)sporulation in haploid as in pat1-114 alone, but no conjugation
ran1/pat1-114 + ste6-(unknown)sporulation in haploid as in pat1-114 alone, but no conjugation
ran1/pat1-114 + ste7-(unknown)same phenotypes as pat1-114 alone - conjugation & sporulation in haploid
ran1/pat1-114 + byr2/ste8-(unknown)same phenotypes as pat1-114 alone - conjugation & sporulation in haploid
ran1/pat1-114 + srw1/ste9-(unknown)same phenotypes as pat1-114 alone - conjugation & sporulation in haploid
ran1/pat1-114 + ste11/aff1-312(unknown)sterile (and not ts as pat1-114 is); abol sporulation


PMID:3442828
win1-1 + cdc25-22 (synonym cdc25.22) + wee1-50 (synonym wee1.50)elongated, minimal med (i.e. more like cdc25-22 alone), normal temp; or rich med, std or high temp
win1-1 + cdc25-22 (synonym cdc25.22) + wee1-50 (synonym wee1.50)normal size (but also has "partially wee") glucose rich med
wee1-50 cdc25-22 win1-1viable rich med, high or std temp, or min med at std temp; inviable minimal med high temp
cf. cdc25-22 + wee1-50wee (as shown previously; presume Ryoko will curate papers)
wee1-50 + win1-1same as win1-1 alone: elongated
cdc25-22 + win1-1more elongated than either single mutant
wee1-6 + win1-1normal size (or very slightly elong) min med std or high temp
wee1-6 + win1-1 cdc25-22mid med: elong std temp; inviable high temp
wee2-3 + win1-1elong min med std or high temp
wee2-3 + win1-1 + cdc25-22elong min med std or high temp


PMID:3448096
cdr2-97 cdc10-129fypo:712 high temp
cdr1-76 cdc10-129fypo:712 high temp
cdr1-76 cdc2-33elongated (lower restrictive temp than cdc2-33 - borderline std/high temp)
cdr2-97 cdc2-33elongated (lower restrictive temp than cdc2-33 - borderline std/high temp)
cdr1-76 cdc25-22elongated, slow growth std temp
cdr2-97 cdc25-22elongated, slow growth std temp
cdr1-76 wee1-50high temp same as wee1-50; std temp larger than wee1-50 but smaller than cdr; high temp elong multiseptate
cdr2-97 wee1-50high temp same as wee1-50; std temp larger than wee1-50 but smaller than cdr; high temp elong multiseptate


PMID:3870979
ran1 pat1-114 in h90 fypo:761 glucose minimal medium std temp (followed by apparently normal sporulation)
pat1-114 in h-normal absence of conjugation minimal? (it's PMA) std temp
pat1-114 in h-normal absence of sporulation PMA std temp
pat1-114 + mei2-16 abol premeiotic dna repl fypo:new1 facs; abol spo micr; high temp
pat1-114 + mei3-17 normal premeiotic dna repl fypo:new2 facs; normal spo micr; high temp
pat1-114 + mei4-2 normal premeiotic dna repl fypo:new2 facs; abol spo micr; high temp
pat1-114 + mei2::LEU2 normal conj micr (h90); abol spo micr (diploid resulting from conj of h90)
ran1::LEU2 + mei2-22viable veg
pat1-114 + mei2-24decr cAMP level std or high temp


PMID:7588609
rep1delta::ura4+ + rep2delta::ura4+abolished sporulation, std temp, micr, high penetrance
rep2delta::ura4+ + res2+ overexpelong veg, std temp, micr; incr duration G1, std temp, facs
rep2delta::ura4+ + res2delta::ura4+normal growth low temp
rep2delta::ura4+ + res1delta::ura4+inviable
epistasisrep2+ overexp no effect on res2delta






Stored additional specificity for evidence (in case we ever want to go back and "upgrade")

PMID:7865880mis5-268(unknown), PCO:0000004FYPO:217PFGE
PMID:8895665orc1 cdc30-4(unknown)FYPO:0000217PFGE
PMID:8838655mcm2FYPO:0000217PFGE
PMID:8838655mcm2FYPO:0001133PFGE
PMID:8838655mcm5FYPO:0000217PFGE
PMID:8838655mcm5FYPO:0001133PFGE
PMID:9914167mcm2 cdc19-P1FYPO:848PFGE
PMID:11606526mcm7-98FYPO:0000848PFGE

Community Curation sessions I've reviewed

PMID:22768263original session c9cf7fe37e5e89fa
sec3-2, exo70deltaabolished prot loc to cell tip fypo:1585; high temp; annotation_extension=assayed_using(PomBase:SPCC622.10c),annotation_extension=assayed_using(PomBase:SPAC6F12.08c)