wiki:WildTypePhenotype

Version 8 (modified by mah79, 5 years ago) (diff)

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This table compiles observable traits of wild-type S. pombe

Nuclear:cytoplasmic volume ratiorange: 0.076 ± 0.013 to 0.089 ± 0.017PMID:17998401

Nuclear:cytoplasmic volume remains constant throughout the cell cycle, and does not depend on DNA content. (PMID:17998401)

Azygotic asci: Usually, under nitrogen starvation conditions haploid cells mate and immediately proceed to meiosis and (zygotic) sporulation. If cells are returned to rich media after conjugation but before commitment to meiosis, diploid cells can grow vegetatively. Nitrogen-starved diploid cells undergo azygotic meiosis and sporulation, resulting in the formation of asci with a characteristic shape. (PMID:18780734)

Arg/N-end rule pathway of protein degradation is present in S. pombe. Detected by different degradation rates of XaaDHFRts–HA–Mcm4 at high temperature, for different amino acid residues at Xaa. Degradation is promoted by Asp, Glu, Arg, or His strongly, and by Trp or Leu weakly. (PMID:23348717)

Mitotic DNA replication forks are organized into replication "factories" each containing several replication forks. About 76 forks are required to replicate the entire genome. (PMID:17304223)

Sporulation phenotypes

  • spore number
  • 4 spores ~80%
  • 3 spores ~ 5%
  • 0 spores ~ 2%
  • 1 spore ~2%